Adm2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Adm2
Entrez Gene ID	223780
Full Name	adrenomedullin 2
Synonyms	Am2,IMD,Imdn
General protein information	Preferred Names adrenomedullin 2 Names ADM2 intermedin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15\|15 E3
Summary	This gene encodes a member of the calcitonin gene-related peptide (CGRP)/calcitonin family of hormones that play a role in the regulation of cardiovascular homeostasis, prolactin release, anti-diuresis, anti-natriuresis, and regulation of food and water intake. The encoded protein is proteolytically processed to generate one or more biologically active peptides. Intravenous injection of the active peptide was found to protect mouse lungs from ischemia/reperfusion injury. [provided by RefSeq, Aug 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_182928.5	NP_891558.1	protein ADM2 preproprotein

Pathways from BioSystems

Homologies

	ADM2	XP_001116328.1
Rattus norvegicus (Norway rat)	Adm2	NP_958829.1
Canis lupus familiaris (dog)	ADM2	XP_848492.2
Pan troglodytes (chimpanzee)	ADM2	XP_001141730.1
Homo sapiens (human)	ADM2	NP_001240774.1
Bos taurus (cattle)	ADM2	NP_001180129.1
Mus musculus (house mouse)	Adm2	NP_891558.1

Gene Ontology
Bibliography

Related articles in PubMed

Intermedin Enlarges the Vascular Lumen by Inducing the Quiescent Endothelial Cell Proliferation.
Wang LJ, Xiao F, Kong LM, Wang DN, Li HY, Wei YG, Tan C, Zhao H, Zhang T, Cao GQ, Zhang K, Wei YQ, Yang HS, Zhang W
Arteriosclerosis, thrombosis, and vascular biology(2017 Dec)

Intermedin reduces neointima formation by regulating vascular smooth muscle cell phenotype via cAMP/PKA pathway.
Zhu Q, Ni XQ, Lu WW, Zhang JS, Ren JL, Wu D, Chen Y, Zhang LS, Yu YR, Tang CS, Qi YF
Atherosclerosis266212-222(2017 Nov)

Adrenomedullin 2 Enhances Beiging in White Adipose Tissue Directly in an Adipocyte-autonomous Manner and Indirectly through Activation of M2 Macrophages.
Lv Y, Zhang SY, Liang X, Zhang H, Xu Z, Liu B, Xu MJ, Jiang C, Shang J, Wang X
The Journal of biological chemistry291(45)23390-23402(2016 Nov)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mouse and human heart valves expressed mRNAs for the CRL ligands adrenomedullin (AM), adrenomedullin-2 (AM-2) and calcitonin gene-related peptide (CGRP) and for their receptor components, i.e., CRL and receptor-activity-modifying proteins 1-3.
Title: Spatial expression of components of a calcitonin receptor-like receptor (CRL) signalling system (CRL, calcitonin gene-related peptide, adrenomedullin, adrenomedullin-2/intermedin) in mouse and human heart valves.

ADM2 enhances subcutaneous White Adipose Tissue beiging via a direct effect by activating the CRLR.RAMP1-cAMP/PKA and p38 MAPK pathways in white adipocytes and via an indirect effect by stimulating alternative M2 polarization in macrophages.
Title: Adrenomedullin 2 Enhances Beiging in White Adipose Tissue Directly in an Adipocyte-autonomous Manner and Indirectly through Activation of M2 Macrophages.

results indicate that endogenous AM2 might be involved in energy metabolism in adipocytes through the upregulation of UCP1 expression
Title: Intermedin/adrenomedullin 2 polypeptide promotes adipose tissue browning and reduces high-fat diet-induced obesity and insulin resistance in mice.

IMD reduces bone resorption by inhibiting osteoblast apoptosis, decreasing the RANKL/OPG ratio and the expression of M-CSF, and inhibiting osteoclast maturation and differentiation.
Title: Effects of intermedin on proliferation, apoptosis and the expression of OPG/RANKL/M-CSF in the MC3T3-E1 osteoblast cell line.

Adrenomedullin-2/intermedin(8-47) ameliorates early ischemia/reperfusion injury in mouse lungs by protecting the integrity of the blood-air barrier and by potently reducing leukocyte influx into the alveolar space
Title: Low-dose adrenomedullin-2/intermedin(8-47) reduces pulmonary ischemia/reperfusion injury.

The following Adm2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Adm2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu08252	NM_182928.5 Latest version!	Mus musculus adrenomedullin 2 (Adm2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Adm2 ( NM_182928.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_891558.1 Mus musculus adrenomedullin 2 (Adm2), mRNA.

Price & Availability↑

CloneID	OMu08252
Clone ID Related Accession (Same CDS sequence)	NM_182928.5
Accession Version	NM_182928.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	protein ADM2 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DV661267.1, CJ052708.1, DV649647.1 and BB705319.1. On Aug 4, 2015 this sequence version replaced NM_182928.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC096428.1, AB121035.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCAGT TGCTGATGGT CACGGTAACC CTCGGTTGCA TCAGCCTCCT CTACCTGCTC 
CCCGGCACGT TGTCTGGCAG CCTGGGCAAG GGACTGAGGC ACTCCAGACC CAGAGAGCCC 
CCAGCTAAGA TTCCTTCCAG TAACCTGCAG CCTGGACACC CTTCCCTTCA GCCTGTAGTC 
TGGAAGTCTC GTCGTCATGC CCCCCAGCCA CAGGGAAGGG GCAACAGGGC CCTTGCTATG 
GTTCATCTGC CTCAGGGTGG TGGCTCACGA CACCCTGGTC CCCAGCGTCC CACGGGATCC 
CGAAGACCCC ATGCCCAGCT CCTGCGGGTT GGCTGTGTAC TGGGTACATG CCAAGTCCAG 
AATCTTAGCC ATCGCCTGTG GCAGCTTGTC CGGCCAGCTG GCCGGCGGGA CTCAGCTCCT 
GTGGATCCCA GCAGCCCCCA CAGTTATGGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_891558.1
CDS	426..878
Translation	MAQLLMVTVTLGCISLLYLLPGTLSGSLGKGLRHSRPREPPAKIPSSNLQPGHPSLQPVVWKSRRHAPQPQGRGNRALAMVHLPQGGGSRHPGPQRPTGSRRPHAQLLRVGCVLGTCQVQNLSHRLWQLVRPAGRRDSAPVDPSSPHSYG

Target ORF information:

RefSeq Version	NM_182928.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus adrenomedullin 2 (Adm2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182928.5

ORF Insert Sequence:

ATGGCCCAGT TGCTGATGGT CACGGTAACC CTCGGTTGCA TCAGCCTCCT CTACCTGCTC 
CCCGGCACGT TGTCTGGCAG CCTGGGCAAG GGACTGAGGC ACTCCAGACC CAGAGAGCCC 
CCAGCTAAGA TTCCTTCCAG TAACCTGCAG CCTGGACACC CTTCCCTTCA GCCTGTAGTC 
TGGAAGTCTC GTCGTCATGC CCCCCAGCCA CAGGGAAGGG GCAACAGGGC CCTTGCTATG 
GTTCATCTGC CTCAGGGTGG TGGCTCACGA CACCCTGGTC CCCAGCGTCC CACGGGATCC 
CGAAGACCCC ATGCCCAGCT CCTGCGGGTT GGCTGTGTAC TGGGTACATG CCAAGTCCAG 
AATCTTAGCC ATCGCCTGTG GCAGCTTGTC CGGCCAGCTG GCCGGCGGGA CTCAGCTCCT 
GTGGATCCCA GCAGCCCCCA CAGTTATGGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Intermedin Enlarges the Vascular Lumen by Inducing the Quiescent Endothelial Cell Proliferation.
Arteriosclerosis, thrombosis, and vascular biology(2017 Dec)
Wang LJ,Xiao F,Kong LM,Wang DN,Li HY,Wei YG,Tan C,Zhao H,Zhang T,Cao GQ,Zhang K,Wei YQ,Yang HS,Zhang W

Intermedin reduces neointima formation by regulating vascular smooth muscle cell phenotype via cAMP/PKA pathway.
Atherosclerosis266212-222(2017 Nov)
Zhu Q,Ni XQ,Lu WW,Zhang JS,Ren JL,Wu D,Chen Y,Zhang LS,Yu YR,Tang CS,Qi YF

Adrenomedullin 2 Enhances Beiging in White Adipose Tissue Directly in an Adipocyte-autonomous Manner and Indirectly through Activation of M2 Macrophages.
The Journal of biological chemistry291(45)23390-23402(2016 Nov)
Lv Y,Zhang SY,Liang X,Zhang H,Xu Z,Liu B,Xu MJ,Jiang C,Shang J,Wang X

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

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Adm2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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