Vpreb2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vpreb2
Entrez Gene ID	22363
Full Name	pre-B lymphocyte gene 2
Synonyms	CD179b,VpreB1,Vpreb-2
General protein information	Preferred Names pre-B lymphocyte gene 2 Names immunoglobulin omega chain Lambda 5
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16 A3\|16 11.14 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_017316944.1	XP_017172433.1	immunoglobulin omega chain isoform X1
XM_017316945.1	XP_017172434.1	immunoglobulin omega chain isoform X1
XM_017316944.2	XP_017172433.1	immunoglobulin omega chain isoform X1
NM_016983.1	NP_058679.1	immunoglobulin omega chain precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Vpreb2	NP_001128260.1
Mus musculus (house mouse)	Vpreb2	NP_058679.1
Canis lupus familiaris (dog)	VPREB1	XP_543537.2
Mus musculus (house mouse)	Vpreb1	NP_058678.1
Rattus norvegicus (Norway rat)	Vpreb1	NP_001102315.1
Homo sapiens (human)	VPREB1	NP_009059.1
Pan troglodytes (chimpanzee)	VPREB1	XP_515010.2
	VPREB1	XP_001089831.1

Gene Ontology
Bibliography

Related articles in PubMed

Surrogate light chain is required for central and peripheral B-cell tolerance and inhibits anti-DNA antibody production by marginal zone B cells.
Ren W, Grimsholm O, Bernardi AI, H??k N, Stern A, Cavallini N, M?rtensson IL
European journal of immunology45(4)1228-37(2015 Apr)

Surrogate light chain expression beyond the pre-B cell stage promotes tolerance in a dose-dependent fashion.
Kil LP, Corneth OB, de Bruijn MJ, Asmawidjaja PS, Krause A, Lubberts E, van Loo PF, Hendriks RW
Journal of autoimmunity5730-41(2015 Feb)

The non-Ig parts of the VpreB and ?5 proteins of the surrogate light chain play opposite roles in the surface representation of the precursor B cell receptor.
Knoll M, Yanagisawa Y, Simmons S, Engels N, Wienands J, Melchers F, Ohnishi K
Journal of immunology (Baltimore, Md. : 1950)188(12)6010-7(2012 Jun)

Distinct genetic control of parasite elimination, dissemination, and disease after Leishmania major infection.
Kurey I, Kobets T, Havelkov? H, Slapnickov? M, Quan L, Trtkov? K, Grekov I, Svobodov? M, Stassen AP, Hutson A, Demant P, Lipoldov? M
Immunogenetics61(9)619-33(2009 Sep)

Only VpreB1, but not VpreB2, is expressed at levels which allow normal development of B cells.
Mundt C, Licence S, Maxwell G, Melchers F, M?rtensson IL
International immunology18(1)163-72(2006 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show no autoantibody production was detectable in surrogate light chain (SLC) components VpreB/CD179a and lambda5/CD179b transgenic mice.
Title: Surrogate light chain expression beyond the pre-B cell stage promotes tolerance in a dose-dependent fashion.

VpreB probes the fitness of newly generated VH domains of IgH chains for later pairing with IgL chains, and its non-Ig part fixes the preBCRs on the surface.
Title: The non-Ig parts of the VpreB and ?5 proteins of the surrogate light chain play opposite roles in the surface representation of the precursor B cell receptor.

Data demonstrate that the pre-B-cell receptor is essential for monoallelic silencing of VpreB and lambda5 at the large pre-BII-cell stage.
Title: The pre-B-cell receptor induces silencing of VpreB and lambda5 transcription.

Efficient binding of a soluble murine pre-B cell receptor to stroma cells requires the non-immunoglobulin-like unique tail of lambda 5.
Title: Interaction of murine precursor B cell receptor with stroma cells is controlled by the unique tail of lambda 5 and stroma cell-associated heparan sulfate.

Mice triply deficient for VpreB2 show B cell developmental defects but functional allelic exclusion of the IgH locus.
Title: VpreB1/VpreB2/lambda 5 triple-deficient mice show impaired B cell development but functional allelic exclusion of the IgH locus.

The following Vpreb2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vpreb2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu10604	NM_016983.1 Latest version!	Mus musculus pre-B lymphocyte gene 2 (Vpreb2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu79306	XM_017316944.2 Latest version!	Mus musculus pre-B lymphocyte gene 2 (Vpreb2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu79306	XM_017316945.1 Latest version!	Mus musculus pre-B lymphocyte gene 2 (Vpreb2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu79306	XM_017316944.1	Mus musculus pre-B lymphocyte gene 2 (Vpreb2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vpreb2 ( NM_016983.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_058679.1 Mus musculus pre-B lymphocyte gene 2 (Vpreb2), mRNA.

Price & Availability↑

CloneID	OMu10604
Clone ID Related Accession (Same CDS sequence)	NM_016983.1
Accession Version	NM_016983.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	immunoglobulin omega chain precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from X05563.1. On Sep 3, 2004 this sequence version replaced XM_489629.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCCTGGA CGTCTGTCCT GCTCATGCTG CTGGCCCACC TCACAGGTTG TGGCCCTCAG 
CCCATGGTGC ATCAGCCACC ATCAGCATCT TCTTCCCTTG GAGCCACCAT CCGCCTCTCC 
TGTACCCTGA GCAACGACCA TAACATTGGC ATTTACAGCA TTTACTGGTA CCAGCAGAGG 
CCGGGCCACC CTCCCAGGTT CCTGCTGAGA TACTTCTCAC ACTCAGACAA GCACCAGGGT 
CCCGATATCC CACCTCGCTT CTCCGGGTCC AAAGATACGG CCAGGAACCT GGGGTATCTG 
AGCATCTCTG AACTGCAGCC TGAGGACGAG GCTGTGTATT ACTGCGCTGT GGGGCTCCGG 
AGCCACGAAA AGAAGAGAAT GGAGAGAGAG TGGGAAGGAG AAAAGTCGTA TACAGATTTG 
GGATCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058679.1
CDS	1..429
Translation	MAWTSVLLMLLAHLTGCGPQPMVHQPPSASSSLGATIRLSCTLSNDHNIGIYSIYWYQQRPGHPPRFLLRYFSHSDKHQGPDIPPRFSGSKDTARNLGYLSISELQPEDEAVYYCAVGLRSHEKKRMEREWEGEKSYTDLGS

Target ORF information:

RefSeq Version	NM_016983.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus pre-B lymphocyte gene 2 (Vpreb2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016983.1

ORF Insert Sequence:

ATGGCCTGGA CGTCTGTCCT GCTCATGCTG CTGGCCCACC TCACAGGTTG TGGCCCTCAG 
CCCATGGTGC ATCAGCCACC ATCAGCATCT TCTTCCCTTG GAGCCACCAT CCGCCTCTCC 
TGTACCCTGA GCAACGACCA TAACATTGGC ATTTACAGCA TTTACTGGTA CCAGCAGAGG 
CCGGGCCACC CTCCCAGGTT CCTGCTGAGA TACTTCTCAC ACTCAGACAA GCACCAGGGT 
CCCGATATCC CACCTCGCTT CTCCGGGTCC AAAGATACGG CCAGGAACCT GGGGTATCTG 
AGCATCTCTG AACTGCAGCC TGAGGACGAG GCTGTGTATT ACTGCGCTGT GGGGCTCCGG 
AGCCACGAAA AGAAGAGAAT GGAGAGAGAG TGGGAAGGAG AAAAGTCGTA TACAGATTTG 
GGATCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vpreb2 ( XM_017316944.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172433.1 Mus musculus pre-B lymphocyte gene 2 (Vpreb2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu79306
Clone ID Related Accession (Same CDS sequence)	XM_017316944.1 , XM_017316945.1 , XM_017316944.2
Accession Version	XM_017316944.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	immunoglobulin omega chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTGGA CGTCTGTCCT GCTCATGCTG CTGGCCCACC TCACAGGTAA GGGAACTCTT 
GGGGTCCAGG GCTTCCTTGC TCCTCCTGTG GCCTTGCTCT GCCCCAGTGA TGGACATGCT 
TCTATCTTCT CAGGTTGTGG CCCTCAGCCC ATGGTGCATC AGCCACCATC AGCATCTTCT 
TCCCTTGGAG CCACCATCCG CCTCTCCTGT ACCCTGAGCA ACGACCATAA CATTGGCATT 
TACAGCATTT ACTGGTACCA GCAGAGGCCG GGCCACCCTC CCAGGTTCCT GCTGAGATAC 
TTCTCACACT CAGACAAGCA CCAGGGTCCC GATATCCCAC CTCGCTTCTC CGGGTCCAAA 
GATACGGCCA GGAACCTGGG GTATCTGAGC ATCTCTGAAC TGCAGCCTGA GGACGAGGCT 
GTGTATTACT GCGCTGTGGG GCTCCGGAGC CACGAAAAGA AGAGAATGGA GAGAGAGTGG 
GAAGGAGAAA AGTCGTATAC AGATTTGGGA TCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172433.1
CDS	398..913
Translation	MAWTSVLLMLLAHLTGKGTLGVQGFLAPPVALLCPSDGHASIFSGCGPQPMVHQPPSASSSLGATIRLSCTLSNDHNIGIYSIYWYQQRPGHPPRFLLRYFSHSDKHQGPDIPPRFSGSKDTARNLGYLSISELQPEDEAVYYCAVGLRSHEKKRMEREWEGEKSYTDLGS

Target ORF information:

RefSeq Version	XM_017316944.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus pre-B lymphocyte gene 2 (Vpreb2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316944.1

ORF Insert Sequence:

ATGGCCTGGA CGTCTGTCCT GCTCATGCTG CTGGCCCACC TCACAGGTAA GGGAACTCTT 
GGGGTCCAGG GCTTCCTTGC TCCTCCTGTG GCCTTGCTCT GCCCCAGTGA TGGACATGCT 
TCTATCTTCT CAGGTTGTGG CCCTCAGCCC ATGGTGCATC AGCCACCATC AGCATCTTCT 
TCCCTTGGAG CCACCATCCG CCTCTCCTGT ACCCTGAGCA ACGACCATAA CATTGGCATT 
TACAGCATTT ACTGGTACCA GCAGAGGCCG GGCCACCCTC CCAGGTTCCT GCTGAGATAC 
TTCTCACACT CAGACAAGCA CCAGGGTCCC GATATCCCAC CTCGCTTCTC CGGGTCCAAA 
GATACGGCCA GGAACCTGGG GTATCTGAGC ATCTCTGAAC TGCAGCCTGA GGACGAGGCT 
GTGTATTACT GCGCTGTGGG GCTCCGGAGC CACGAAAAGA AGAGAATGGA GAGAGAGTGG 
GAAGGAGAAA AGTCGTATAC AGATTTGGGA TCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vpreb2 ( XM_017316945.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172434.1 Mus musculus pre-B lymphocyte gene 2 (Vpreb2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu79306
Clone ID Related Accession (Same CDS sequence)	XM_017316944.1 , XM_017316945.1 , XM_017316944.2
Accession Version	XM_017316945.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	immunoglobulin omega chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTGGA CGTCTGTCCT GCTCATGCTG CTGGCCCACC TCACAGGTAA GGGAACTCTT 
GGGGTCCAGG GCTTCCTTGC TCCTCCTGTG GCCTTGCTCT GCCCCAGTGA TGGACATGCT 
TCTATCTTCT CAGGTTGTGG CCCTCAGCCC ATGGTGCATC AGCCACCATC AGCATCTTCT 
TCCCTTGGAG CCACCATCCG CCTCTCCTGT ACCCTGAGCA ACGACCATAA CATTGGCATT 
TACAGCATTT ACTGGTACCA GCAGAGGCCG GGCCACCCTC CCAGGTTCCT GCTGAGATAC 
TTCTCACACT CAGACAAGCA CCAGGGTCCC GATATCCCAC CTCGCTTCTC CGGGTCCAAA 
GATACGGCCA GGAACCTGGG GTATCTGAGC ATCTCTGAAC TGCAGCCTGA GGACGAGGCT 
GTGTATTACT GCGCTGTGGG GCTCCGGAGC CACGAAAAGA AGAGAATGGA GAGAGAGTGG 
GAAGGAGAAA AGTCGTATAC AGATTTGGGA TCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172434.1
CDS	523..1038
Translation	MAWTSVLLMLLAHLTGKGTLGVQGFLAPPVALLCPSDGHASIFSGCGPQPMVHQPPSASSSLGATIRLSCTLSNDHNIGIYSIYWYQQRPGHPPRFLLRYFSHSDKHQGPDIPPRFSGSKDTARNLGYLSISELQPEDEAVYYCAVGLRSHEKKRMEREWEGEKSYTDLGS

Target ORF information:

RefSeq Version	XM_017316945.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus pre-B lymphocyte gene 2 (Vpreb2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316945.1

ORF Insert Sequence:

ATGGCCTGGA CGTCTGTCCT GCTCATGCTG CTGGCCCACC TCACAGGTAA GGGAACTCTT 
GGGGTCCAGG GCTTCCTTGC TCCTCCTGTG GCCTTGCTCT GCCCCAGTGA TGGACATGCT 
TCTATCTTCT CAGGTTGTGG CCCTCAGCCC ATGGTGCATC AGCCACCATC AGCATCTTCT 
TCCCTTGGAG CCACCATCCG CCTCTCCTGT ACCCTGAGCA ACGACCATAA CATTGGCATT 
TACAGCATTT ACTGGTACCA GCAGAGGCCG GGCCACCCTC CCAGGTTCCT GCTGAGATAC 
TTCTCACACT CAGACAAGCA CCAGGGTCCC GATATCCCAC CTCGCTTCTC CGGGTCCAAA 
GATACGGCCA GGAACCTGGG GTATCTGAGC ATCTCTGAAC TGCAGCCTGA GGACGAGGCT 
GTGTATTACT GCGCTGTGGG GCTCCGGAGC CACGAAAAGA AGAGAATGGA GAGAGAGTGG 
GAAGGAGAAA AGTCGTATAC AGATTTGGGA TCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vpreb2 ( XM_017316944.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172433.1 Mus musculus pre-B lymphocyte gene 2 (Vpreb2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu79306
Clone ID Related Accession (Same CDS sequence)	XM_017316944.1 , XM_017316945.1 , XM_017316944.2
Accession Version	XM_017316944.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	immunoglobulin omega chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316944.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTGGA CGTCTGTCCT GCTCATGCTG CTGGCCCACC TCACAGGTAA GGGAACTCTT 
GGGGTCCAGG GCTTCCTTGC TCCTCCTGTG GCCTTGCTCT GCCCCAGTGA TGGACATGCT 
TCTATCTTCT CAGGTTGTGG CCCTCAGCCC ATGGTGCATC AGCCACCATC AGCATCTTCT 
TCCCTTGGAG CCACCATCCG CCTCTCCTGT ACCCTGAGCA ACGACCATAA CATTGGCATT 
TACAGCATTT ACTGGTACCA GCAGAGGCCG GGCCACCCTC CCAGGTTCCT GCTGAGATAC 
TTCTCACACT CAGACAAGCA CCAGGGTCCC GATATCCCAC CTCGCTTCTC CGGGTCCAAA 
GATACGGCCA GGAACCTGGG GTATCTGAGC ATCTCTGAAC TGCAGCCTGA GGACGAGGCT 
GTGTATTACT GCGCTGTGGG GCTCCGGAGC CACGAAAAGA AGAGAATGGA GAGAGAGTGG 
GAAGGAGAAA AGTCGTATAC AGATTTGGGA TCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172433.1
CDS	393..908
Translation	MAWTSVLLMLLAHLTGKGTLGVQGFLAPPVALLCPSDGHASIFSGCGPQPMVHQPPSASSSLGATIRLSCTLSNDHNIGIYSIYWYQQRPGHPPRFLLRYFSHSDKHQGPDIPPRFSGSKDTARNLGYLSISELQPEDEAVYYCAVGLRSHEKKRMEREWEGEKSYTDLGS

Target ORF information:

RefSeq Version	XM_017316944.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus pre-B lymphocyte gene 2 (Vpreb2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316944.2

ORF Insert Sequence:

ATGGCCTGGA CGTCTGTCCT GCTCATGCTG CTGGCCCACC TCACAGGTAA GGGAACTCTT 
GGGGTCCAGG GCTTCCTTGC TCCTCCTGTG GCCTTGCTCT GCCCCAGTGA TGGACATGCT 
TCTATCTTCT CAGGTTGTGG CCCTCAGCCC ATGGTGCATC AGCCACCATC AGCATCTTCT 
TCCCTTGGAG CCACCATCCG CCTCTCCTGT ACCCTGAGCA ACGACCATAA CATTGGCATT 
TACAGCATTT ACTGGTACCA GCAGAGGCCG GGCCACCCTC CCAGGTTCCT GCTGAGATAC 
TTCTCACACT CAGACAAGCA CCAGGGTCCC GATATCCCAC CTCGCTTCTC CGGGTCCAAA 
GATACGGCCA GGAACCTGGG GTATCTGAGC ATCTCTGAAC TGCAGCCTGA GGACGAGGCT 
GTGTATTACT GCGCTGTGGG GCTCCGGAGC CACGAAAAGA AGAGAATGGA GAGAGAGTGG 
GAAGGAGAAA AGTCGTATAC AGATTTGGGA TCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Surrogate light chain is required for central and peripheral B-cell tolerance and inhibits anti-DNA antibody production by marginal zone B cells.
European journal of immunology45(4)1228-37(2015 Apr)
Ren W,Grimsholm O,Bernardi AI,H??k N,Stern A,Cavallini N,M?rtensson IL

Surrogate light chain expression beyond the pre-B cell stage promotes tolerance in a dose-dependent fashion.
Journal of autoimmunity5730-41(2015 Feb)
Kil LP,Corneth OB,de Bruijn MJ,Asmawidjaja PS,Krause A,Lubberts E,van Loo PF,Hendriks RW

The non-Ig parts of the VpreB and ?5 proteins of the surrogate light chain play opposite roles in the surface representation of the precursor B cell receptor.
Journal of immunology (Baltimore, Md. : 1950)188(12)6010-7(2012 Jun)
Knoll M,Yanagisawa Y,Simmons S,Engels N,Wienands J,Melchers F,Ohnishi K

Distinct genetic control of parasite elimination, dissemination, and disease after Leishmania major infection.
Immunogenetics61(9)619-33(2009 Sep)
Kurey I,Kobets T,Havelkov? H,Slapnickov? M,Quan L,Trtkov? K,Grekov I,Svobodov? M,Stassen AP,Hutson A,Demant P,Lipoldov? M

Only VpreB1, but not VpreB2, is expressed at levels which allow normal development of B cells.
International immunology18(1)163-72(2006 Jan)
Mundt C,Licence S,Maxwell G,Melchers F,M?rtensson IL

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Vpreb2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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