Ubb cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ubb
Entrez Gene ID	22187
Full Name	ubiquitin B
Synonyms	AL033289,Rps27a,Uba52,Ubb2,Ubc
General protein information	Preferred Names ubiquitin B Names polyubiquitin-B
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 B2\|11 38.46 cM
Summary	This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of four direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Pseudogenes of this gene are located on chromosomes 3 and 14. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001313984.1	NP_001300913.1	polyubiquitin-B
NM_011664.4	NP_035794.1	polyubiquitin-B

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	UBQ14	NP_849292.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002599	XP_312337.4
Mus musculus (house mouse)	Ubb	NP_035794.1
Drosophila melanogaster (fruit fly)	CG11700	NP_572306.1
Rattus norvegicus (Norway rat)	Ubb	NP_620250.1
Gallus gallus (chicken)	LOC101747587	XP_004946719.1
Bos taurus (cattle)	UBB	NP_776558.1
Arabidopsis thaliana (thale cress)	UBQ3	NP_001031824.1
Xenopus tropicalis (tropical clawed frog)	ubc	XP_004910600.1

Gene Ontology
Bibliography

Related articles in PubMed

PLAA Mutations Cause a Lethal Infantile Epileptic Encephalopathy by Disrupting Ubiquitin-Mediated Endolysosomal Degradation of Synaptic Proteins.
Hall EA, Nahorski MS, Murray LM, Shaheen R, Perkins E, Dissanayake KN, Kristaryanto Y, Jones RA, Vogt J, Rivagorda M, Handley MT, Mali GR, Quidwai T, Soares DC, Keighren MA, McKie L, Mort RL, Gammoh N, Garcia-Munoz A, Davey T, Vermeren M, Walsh D, Budd P, Aligianis IA, Faqeih E, Quigley AJ, Jackson IJ, Kulathu Y, Jackson M, Ribchester RR, von Kriegsheim A, Alkuraya FS, Woods CG, Maher ER, Mill P
American journal of human genetics100(5)706-724(2017 May)

Comparative expression profiling of testis-enriched genes regulated during the development of spermatogonial cells.
Ahn J, Park YJ, Chen P, Lee TJ, Jeon YJ, Croce CM, Suh Y, Hwang S, Kwon WS, Pang MG, Kim CH, Lee SS, Lee K
PloS one12(4)e0175787(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that levels of repressor element-1 silencing transcription factor (REST) are elevated in polyubiquitin-B knockout (Ubb -/-) cells.
Title: Regulation of REST levels overcomes dysregulation of neural stem cell differentiation caused by disruption of polyubiquitin gene Ubb.

Ubb deficiency causes dysregulation of neural stem cell differentiation with premature gliogenesis.
Title: Disruption of polyubiquitin gene Ubb causes dysregulation of neural stem cell differentiation with premature gliogenesis.

study suggests that Ub homeostasis, or the maintenance of cellular free Ub above certain threshold levels, is essential for proper neuronal development and survival
Title: Restoration of cellular ubiquitin reverses impairments in neuronal development caused by disruption of the polyubiquitin gene Ubb.

The lysine 6 to tryptophan ubiquitin substitution results in the cataract development in newborn mice.
Title: Newborn mouse lens proteome and its alteration by lysine 6 mutant ubiquitin.

upon stress induced by disruption of Ubb, neuronal vulnerability may be determined based on the ability of neurons or neighboring cells to maintain free Ub levels for the protection of neuronal function and survival.
Title: Locus coeruleus neurons are resistant to dysfunction and degeneration by maintaining free ubiquitin levels although total ubiquitin levels decrease upon disruption of polyubiquitin gene Ubb.

The following Ubb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ubb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu08569	NM_011664.4 Latest version!	Mus musculus ubiquitin B (Ubb), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
OMu08569	NM_001313984.1 Latest version!	Mus musculus ubiquitin B (Ubb), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ubb ( NM_001313984.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001300913.1 Mus musculus ubiquitin B (Ubb), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu08569
Clone ID Related Accession (Same CDS sequence)	NM_001313984.1 , NM_011664.4
Accession Version	NM_001313984.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Mus musculus(house mouse)
Product	polyubiquitin-B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY083861.1, AK007273.1 and AI836769.1. On Sep 19, 2015 this sequence version replaced XM_006533159.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BU936829.1, BU946042.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGATCT TCGTGAAGAC CCTGACCGGC AAGACCATCA CCCTAGAGGT GGAGCCCAGT 
GACACCATCG AGAACGTGAA GGCCAAGATC CAGGATAAAG AGGGCATCCC CCCTGACCAG 
CAGAGGCTGA TCTTTGCCGG CAAGCAGCTG GAAGATGGCC GCACCCTCTC TGATTACAAC 
ATCCAGAAGG AGTCAACCCT GCACCTGGTC CTTCGCCTGA GAGGTGGCAT GCAGATCTTC 
GTGAAGACCC TGACCGGCAA GACCATCACC CTGGAGGTGG AGCCCAGTGA CACCATCGAG 
AACGTGAAGG CCAAGATCCA GGATAAAGAG GGCATCCCCC CTGACCAGCA GAGGCTGATC 
TTTGCCGGCA AGCAGCTGGA AGATGGCCGC ACCCTCTCTG ATTACAACAT CCAGAAAGAG 
TCAACCCTGC ACCTGGTCCT CCGTCTGAGG GGTGGCATGC AGATCTTCGT GAAGACCCTG 
ACTGGCAAGA CCATCACCCT GGAGGTGGAG CCCAGTGACA CCATCGAGAA CGTGAAGGCC 
AAGATCCAGG ATAAAGAGGG CATCCCCCCT GACCAGCAGA GGCTGATCTT TGCCGGCAAG 
CAGCTGGAAG ATGGCCGCAC CCTCTCTGAT TACAACATCC AGAAGGAGTC AACCCTGCAC 
CTGGTCCTTC GCCTGAGAGG TGGCATGCAG ATCTTCGTGA AGACCCTGAC CGGCAAGACC 
ATCACCTTGG AGGTGGAGCC CAGTGACACC ATCGAGAATG TGAAGGCCAA GATCCAGGAT 
AAAGAGGGCA TCCCCCCTGA CCAGCAGAGG CTGATCTTTG CCGGCAAGCA GCTAGAAGAT 
GGCCGCACTC TCTCTGATTA CAACATCCAG AAAGAGTCGA CCCTGCACCT GGTCCTCCGT 
CTGAGGGGTG GCTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300913.1
CDS	128..1045
Translation	MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGY

Target ORF information:

RefSeq Version	NM_001313984.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin B (Ubb), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313984.1

ORF Insert Sequence:

ATGCAGATCT TCGTGAAGAC CCTGACCGGC AAGACCATCA CCCTAGAGGT GGAGCCCAGT 
GACACCATCG AGAACGTGAA GGCCAAGATC CAGGATAAAG AGGGCATCCC CCCTGACCAG 
CAGAGGCTGA TCTTTGCCGG CAAGCAGCTG GAAGATGGCC GCACCCTCTC TGATTACAAC 
ATCCAGAAGG AGTCAACCCT GCACCTGGTC CTTCGCCTGA GAGGTGGCAT GCAGATCTTC 
GTGAAGACCC TGACCGGCAA GACCATCACC CTGGAGGTGG AGCCCAGTGA CACCATCGAG 
AACGTGAAGG CCAAGATCCA GGATAAAGAG GGCATCCCCC CTGACCAGCA GAGGCTGATC 
TTTGCCGGCA AGCAGCTGGA AGATGGCCGC ACCCTCTCTG ATTACAACAT CCAGAAAGAG 
TCAACCCTGC ACCTGGTCCT CCGTCTGAGG GGTGGCATGC AGATCTTCGT GAAGACCCTG 
ACTGGCAAGA CCATCACCCT GGAGGTGGAG CCCAGTGACA CCATCGAGAA CGTGAAGGCC 
AAGATCCAGG ATAAAGAGGG CATCCCCCCT GACCAGCAGA GGCTGATCTT TGCCGGCAAG 
CAGCTGGAAG ATGGCCGCAC CCTCTCTGAT TACAACATCC AGAAGGAGTC AACCCTGCAC 
CTGGTCCTTC GCCTGAGAGG TGGCATGCAG ATCTTCGTGA AGACCCTGAC CGGCAAGACC 
ATCACCTTGG AGGTGGAGCC CAGTGACACC ATCGAGAATG TGAAGGCCAA GATCCAGGAT 
AAAGAGGGCA TCCCCCCTGA CCAGCAGAGG CTGATCTTTG CCGGCAAGCA GCTAGAAGAT 
GGCCGCACTC TCTCTGATTA CAACATCCAG AAAGAGTCGA CCCTGCACCT GGTCCTCCGT 
CTGAGGGGTG GCTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ubb ( NM_011664.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035794.1 Mus musculus ubiquitin B (Ubb), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu08569
Clone ID Related Accession (Same CDS sequence)	NM_001313984.1 , NM_011664.4
Accession Version	NM_011664.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Mus musculus(house mouse)
Product	polyubiquitin-B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DV653535.1, AK007273.1 and AI836769.1. On Sep 19, 2015 this sequence version replaced NM_011664.3. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X51703.1, AK007273.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGATCT TCGTGAAGAC CCTGACCGGC AAGACCATCA CCCTAGAGGT GGAGCCCAGT 
GACACCATCG AGAACGTGAA GGCCAAGATC CAGGATAAAG AGGGCATCCC CCCTGACCAG 
CAGAGGCTGA TCTTTGCCGG CAAGCAGCTG GAAGATGGCC GCACCCTCTC TGATTACAAC 
ATCCAGAAGG AGTCAACCCT GCACCTGGTC CTTCGCCTGA GAGGTGGCAT GCAGATCTTC 
GTGAAGACCC TGACCGGCAA GACCATCACC CTGGAGGTGG AGCCCAGTGA CACCATCGAG 
AACGTGAAGG CCAAGATCCA GGATAAAGAG GGCATCCCCC CTGACCAGCA GAGGCTGATC 
TTTGCCGGCA AGCAGCTGGA AGATGGCCGC ACCCTCTCTG ATTACAACAT CCAGAAAGAG 
TCAACCCTGC ACCTGGTCCT CCGTCTGAGG GGTGGCATGC AGATCTTCGT GAAGACCCTG 
ACTGGCAAGA CCATCACCCT GGAGGTGGAG CCCAGTGACA CCATCGAGAA CGTGAAGGCC 
AAGATCCAGG ATAAAGAGGG CATCCCCCCT GACCAGCAGA GGCTGATCTT TGCCGGCAAG 
CAGCTGGAAG ATGGCCGCAC CCTCTCTGAT TACAACATCC AGAAGGAGTC AACCCTGCAC 
CTGGTCCTTC GCCTGAGAGG TGGCATGCAG ATCTTCGTGA AGACCCTGAC CGGCAAGACC 
ATCACCTTGG AGGTGGAGCC CAGTGACACC ATCGAGAATG TGAAGGCCAA GATCCAGGAT 
AAAGAGGGCA TCCCCCCTGA CCAGCAGAGG CTGATCTTTG CCGGCAAGCA GCTAGAAGAT 
GGCCGCACTC TCTCTGATTA CAACATCCAG AAAGAGTCGA CCCTGCACCT GGTCCTCCGT 
CTGAGGGGTG GCTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035794.1
CDS	432..1349
Translation	MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGY

Target ORF information:

RefSeq Version	NM_011664.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin B (Ubb), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011664.4

ORF Insert Sequence:

ATGCAGATCT TCGTGAAGAC CCTGACCGGC AAGACCATCA CCCTAGAGGT GGAGCCCAGT 
GACACCATCG AGAACGTGAA GGCCAAGATC CAGGATAAAG AGGGCATCCC CCCTGACCAG 
CAGAGGCTGA TCTTTGCCGG CAAGCAGCTG GAAGATGGCC GCACCCTCTC TGATTACAAC 
ATCCAGAAGG AGTCAACCCT GCACCTGGTC CTTCGCCTGA GAGGTGGCAT GCAGATCTTC 
GTGAAGACCC TGACCGGCAA GACCATCACC CTGGAGGTGG AGCCCAGTGA CACCATCGAG 
AACGTGAAGG CCAAGATCCA GGATAAAGAG GGCATCCCCC CTGACCAGCA GAGGCTGATC 
TTTGCCGGCA AGCAGCTGGA AGATGGCCGC ACCCTCTCTG ATTACAACAT CCAGAAAGAG 
TCAACCCTGC ACCTGGTCCT CCGTCTGAGG GGTGGCATGC AGATCTTCGT GAAGACCCTG 
ACTGGCAAGA CCATCACCCT GGAGGTGGAG CCCAGTGACA CCATCGAGAA CGTGAAGGCC 
AAGATCCAGG ATAAAGAGGG CATCCCCCCT GACCAGCAGA GGCTGATCTT TGCCGGCAAG 
CAGCTGGAAG ATGGCCGCAC CCTCTCTGAT TACAACATCC AGAAGGAGTC AACCCTGCAC 
CTGGTCCTTC GCCTGAGAGG TGGCATGCAG ATCTTCGTGA AGACCCTGAC CGGCAAGACC 
ATCACCTTGG AGGTGGAGCC CAGTGACACC ATCGAGAATG TGAAGGCCAA GATCCAGGAT 
AAAGAGGGCA TCCCCCCTGA CCAGCAGAGG CTGATCTTTG CCGGCAAGCA GCTAGAAGAT 
GGCCGCACTC TCTCTGATTA CAACATCCAG AAAGAGTCGA CCCTGCACCT GGTCCTCCGT 
CTGAGGGGTG GCTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PLAA Mutations Cause a Lethal Infantile Epileptic Encephalopathy by Disrupting Ubiquitin-Mediated Endolysosomal Degradation of Synaptic Proteins.
American journal of human genetics100(5)706-724(2017 May)
Hall EA,Nahorski MS,Murray LM,Shaheen R,Perkins E,Dissanayake KN,Kristaryanto Y,Jones RA,Vogt J,Rivagorda M,Handley MT,Mali GR,Quidwai T,Soares DC,Keighren MA,McKie L,Mort RL,Gammoh N,Garcia-Munoz A,Davey T,Vermeren M,Walsh D,Budd P,Aligianis IA,Faqeih E,Quigley AJ,Jackson IJ,Kulathu Y,Jackson M,Ribchester RR,von Kriegsheim A,Alkuraya FS,Woods CG,Maher ER,Mill P

Comparative expression profiling of testis-enriched genes regulated during the development of spermatogonial cells.
PloS one12(4)e0175787(2017)
Ahn J,Park YJ,Chen P,Lee TJ,Jeon YJ,Croce CM,Suh Y,Hwang S,Kwon WS,Pang MG,Kim CH,Lee SS,Lee K

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Ubb cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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