Trim24 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trim24
Entrez Gene ID	21848
Full Name	tripartite motif-containing 24
Synonyms	A130082H20Rik,AI447469,D430004I05Rik,TIF1,TIF1-alpha,TIF1alpha,Tif1a
General protein information	Preferred Names tripartite motif-containing 24 Names transcription intermediary factor 1-alpha E3 ubiquitin-protein ligase Trim24 RING-type E3 ubiquitin transferase TIF1-alpha transcriptional intermediary factor 1, alpha tripartite motif-containing protein 24
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6\|6 B1
Summary	The protein encoded by this gene is part of the tripartite-motif containing family (TRIM), which are typified by the RING, B-box type 1, B-box type 2, and coiled-coil region domains. This protein, which also contains a PHD/TTC finger and bromodomain important for regulating nuclear receptors and binding chromatin, has important roles in differentiation, development, and tissue homeostasis. This protein has been reported to regulate the activity of the tumor suppressor p53 and of the retinoic acid receptor. A translocation event between this gene and Braf transforming gene, which results in the fusion protein T18, has been reported in hepatocellular carcinomas. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006505895.2	XP_006505958.1	transcription intermediary factor 1-alpha isoform X1
NM_001272064.1	NP_001258993.1	transcription intermediary factor 1-alpha isoform 2
NM_001272076.1	NP_001259005.1	transcription intermediary factor 1-alpha isoform 3
XM_006505895.3	XP_006505958.1	transcription intermediary factor 1-alpha isoform X1
NM_145076.4	NP_659542.3	transcription intermediary factor 1-alpha isoform 1

Pathways from BioSystems

Homologies

	TRIM24	XP_001107279.1
Canis lupus familiaris (dog)	TRIM24	XP_003639599.2
Bos taurus (cattle)	TRIM24	XP_002687040.1
Mus musculus (house mouse)	Trim24	NP_659542.3
Xenopus tropicalis (tropical clawed frog)	trim24	NP_001121448.1
Homo sapiens (human)	TRIM24	NP_056989.2
Pan troglodytes (chimpanzee)	TRIM24	XP_519410.2
Gallus gallus (chicken)	TRIM24	XP_416340.4

Gene Ontology
Bibliography

Related articles in PubMed

Expanding the Circuitry of Pluripotency by Selective Isolation of Chromatin-Associated Proteins.
Rafiee MR, Girardot C, Sigismondo G, Krijgsveld J
Molecular cell64(3)624-635(2016 Nov)

Novel gene function revealed by mouse mutagenesis screens for models of age-related disease.
Potter PK, Bowl MR, Jeyarajan P, Wisby L, Blease A, Goldsworthy ME, Simon MM, Greenaway S, Michel V, Barnard A, Aguilar C, Agnew T, Banks G, Blake A, Chessum L, Dorning J, Falcone S, Goosey L, Harris S, Haynes A, Heise I, Hillier R, Hough T, Hoslin A, Hutchison M, King R, Kumar S, Lad HV, Law G, MacLaren RE, Morse S, Nicol T, Parker A, Pickford K, Sethi S, Starbuck B, Stelma F, Cheeseman M, Cross SH, Foster RG, Jackson IJ, Peirson SN, Thakker RV, Vincent T, Scudamore C, Wells S, El-Amraoui A, Petit C, Acevedo-Arozena A, Nolan PM, Cox R, Mallon AM, Brown SD
Nature communications712444(2016 Aug)

T-cell-intrinsic Tif1?/Trim24 regulates IL-1R expression on TH2 cells and TH2 cell-mediated airway allergy.
Perez-Lloret J, Okoye IS, Guidi R, Kannan Y, Coomes SM, Czieso S, Mengus G, Davidson I, Wilson MS
Proceedings of the National Academy of Sciences of the United States of America113(5)E568-76(2016 Feb)

Trim24-repressed VL30 retrotransposons regulate gene expression by producing noncoding RNA.
Herquel B, Ouararhni K, Martianov I, Le Gras S, Ye T, Keime C, Lerouge T, Jost B, Cammas F, Losson R, Davidson I
Nature structural & molecular biology20(3)339-46(2013 Mar)

Interaction with members of the heterochromatin protein 1 (HP1) family and histone deacetylation are differentially involved in transcriptional silencing by members of the TIF1 family.
Nielsen AL, Ortiz JA, You J, Oulad-Abdelghani M, Khechumian R, Gansmuller A, Chambon P, Losson R
The EMBO journal18(22)6385-95(1999 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Maintenance of pluripotency is regulated by a network of transcription factors coordinated by Oct4, Sox2, and Nanog; Trim24 significantly improved efficiency of cellular reprogramming, demonstrating its direct functionality in establishing pluripotency.
Title: Expanding the Circuitry of Pluripotency by Selective Isolation of Chromatin-Associated Proteins.

data identify a previously unappreciated Trim24-dependent requirement for IL-1R expression on TH2 cells and an important nonredundant role for T-cell-intrinsic Trim24 in TH2-mediated allergy and antihelminth immunity
Title: T-cell-intrinsic Tif1?/Trim24 regulates IL-1R expression on TH2 cells and TH2 cell-mediated airway allergy.

Trim24 repressed VL30-class endogenous retroviruses retrotransposons
Title: Trim24-repressed VL30 retrotransposons regulate gene expression by producing noncoding RNA.

These results identify Trim24 as a novel negative regulator of the IFN/STAT pathway and suggest that this repression through Rara inhibition may prevent liver cancer.
Title: Tripartite motif 24 (Trim24/Tif1?) tumor suppressor protein is a novel negative regulator of interferon (IFN)/signal transducers and activators of transcription (STAT) signaling pathway acting through retinoic acid receptor ? (Rar?) inhibition.

Somatic hepatocyte-specific inactivation of TRIM24, TRIM28, or TRIM33 all promote hepatocellular carcinoma in a cell-autonomous manner in mice.
Title: Transcription cofactors TRIM24, TRIM28, and TRIM33 associate to form regulatory complexes that suppress murine hepatocellular carcinoma.

The following Trim24 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trim24 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu17041	NM_001272076.1 Latest version!	Mus musculus tripartite motif-containing 24 (Trim24), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu37118	XM_006505895.3 Latest version!	Mus musculus tripartite motif-containing 24 (Trim24), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu12128	NM_145076.4 Latest version!	Mus musculus tripartite motif-containing 24 (Trim24), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$615.30 $879.00
OMu17139	NM_001272064.1 Latest version!	Mus musculus tripartite motif-containing 24 (Trim24), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
View Old Accession Versions >>
OMu37118	XM_006505895.2	Mus musculus tripartite motif-containing 24 (Trim24), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trim24 ( XM_006505895.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006505958.1 Mus musculus tripartite motif-containing 24 (Trim24), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu37118
Clone ID Related Accession (Same CDS sequence)	XM_006505895.2 , XM_006505895.3
Accession Version	XM_006505895.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	transcription intermediary factor 1-alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006505895.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAAA CAAAATATAT AAAGTATACA GGAAATCAGA TCCAAAATAG GATAATTGAA 
ATAAATCAAA ACCAAAAGCA GGTGGAACAG GATATTAAAG TTGCCATCTT CACATTGATG 
GTGGAGATAA ACAAAAAAGG GAAAGCTCTG CTGCACCAGC TTGAGAGTCT TGCAAAGGAC 
CATCGAATGA AACTCATGCA ACAACAGCAG GAAGTGGCTG GGCTTTCTAA GCAGTTAGAG 
CACGTCATGC ATTTTTCTAA ATGGGCTGTT TCCAGTGGCA GCAGCACAGC CTTGCTGTAC 
AGCAAGCGGC TGATTACATA CAGGTTACGG CACCTTCTTC GTGCAAGGTG TGATGCTTCT 
CCTGTGACCA ACACCACCAT CCAGTTTCAC TGTGATCCTA GTTTCTGGGC TCAAAATATT 
ATCAACTTGG GTTCTTTAGT AATCGAGGAT AAAGAGAGCC AGCCACAAAT GCCTAAGCAG 
AATCCTGTCG TGGAGCAGAG TTCACAGCCA CCAGGTGGTT TACCTTCCAA CCAGTTATCC 
AAGTTCCCAA CACAGATCAG CCTAGCTCAG TTACGACTCC AGCATATTCA GCAACAGGTA 
ATGGCTCAGA GGCAACAGGT GCAACGGAGG CCAGCACCTG TGGGTTTACC AAACCCTAGA 
ATGCAGGGGC CCATCCAGCA GCCTTCCATC TCTCATCAGC ATCCGCCACC ACGCTTAATA 
AACTTTCAGA ATCACAGCCC TAAGCCCAAT GGACCAGTTC TTCCTCCTTA TCCTCAGCAG 
CTGAGATATT CACCAAGCCA GAATGTACCT CGGCAGACAA CAATAAAGCC CAACCCCTTG 
CAAATGGCTT TTTTGGCTCA ACAGGCCATA AAACAGTGGC AGATCAGCAG TGTACAGGCT 
CCGCCCACAA CTGCCAGCAG CTCCTCCTCC ACGCCGTCCA GCCCCACAAT CACAAGTGCA 
GCTGGGTACG ATGGAAAAGC TTTTAGTTCA CCCATGATTG ATCTGAGTGC ACCGGTGGGA 
GGGTCTTACA ATCTTCCTTC TCTTCCAGAT ATTGATTGTT CAAGTACTAT AATGTTGGAC 
AACATTGCAA GGAAAGACAC AGGTGTAGAT CACGCCCAGC CGAGGCCTCC GTCAAACAGA 
ACGGTGCAGT CACCAAATTC ATCAGTGCCA TCTCCAGGCC TTGCAGGGCC TGTTACTATG 
ACTAGCGTCC ATCCCCCAAT ACGTTCACCT AGTGCCTCCA GTGTTGGAAG TCGAGGAAGC 
TCTGGCTCTT CCAGCAAACC AGCAGGAGCT GATTCTACTC ACAAGGTCCC AGTAGTCATG 
CTGGAGCCAA TTCGAATAAA ACAGGAAAAC AGTGGACCAC CTGAAAATTA TGATTTTCCT 
GTTGTTATAG TAAAACAAGA ATCAGATGAA GAATCTAGAC CTCAAAATAC TAACTATCCA 
AGAAGCATAC TTACCTCCCT CCTCTTAAAC AGCAGTCAGA GCTCTGCTTC TGAGGAAACC 
GTGTTACGAT CTGATGCCCC TGATAGTACA GGAGATCAGC CTGGACTCCA TCAAGAAAAT 
TCCTCAAATG GAAAGTCTGA GTGGTCGGAT GCCTCCCAGA AGTCCCCTGT GCATGTCGGA 
GAGACGAGGA AGGAGGATGA CCCCAATGAA GACTGGTGTG CTGTTTGTCA AAATGGTGGG 
GAACTCCTAT GCTGTGAGAA ATGTCCTAAA GTATTCCATC TTACTTGTCA TGTGCCCACC 
TTGACAAATT TTCCAAGTGG AGAATGGATC TGTACTTTCT GCCGAGACTT ATCTAAGCCA 
GAGGTTGACT ATGATTGTGA TGTTCCCAGT CACCACTCAG AGAAACGGAA AAGTGAAGGC 
CTTACTAAGT TAACGCCAAT AGACAAAAGG AAATGTGAAC GCCTACTTCT GTTTCTTTAC 
TGCCATGAAA TGAGCCTGGC TTTCCAAGAC CCTGTTCCTC TAACTGTGCC TGATTATTAT 
AAAATAATTA AAAACCCAAT GGACTTGTCA ACCATCAAGA AAAGACTTCA GGAGGATTAT 
TGCATGTATA CAAAGCCTGA AGACTTTGTA GCTGATTTTA GATTGATCTT TCAAAACTGT 
GCTGAATTCA ATGAGCCTGA TTCTGAAGTA GCCAATGCTG GTATAAAACT TGAAAGCTAT 
TTTGAAGAAC TTCTAAAGAA TCTTTATCCA GAAAAAAGGT TTCCTAAGGT AGAATTCAGG 
CATGAAGCAG AAGACTGTAA GTTCAGTGAC GACTCAGACG ATGACTTTGT ACAGCCCCGG 
AAGAAGCGTC TCAAGAGCAC CGAGGATCGC CAGCTGCTTA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006505958.1
CDS	1915..4239
Translation	MEKTKYIKYTGNQIQNRIIEINQNQKQVEQDIKVAIFTLMVEINKKGKALLHQLESLAKDHRMKLMQQQQEVAGLSKQLEHVMHFSKWAVSSGSSTALLYSKRLITYRLRHLLRARCDASPVTNTTIQFHCDPSFWAQNIINLGSLVIEDKESQPQMPKQNPVVEQSSQPPGGLPSNQLSKFPTQISLAQLRLQHIQQQVMAQRQQVQRRPAPVGLPNPRMQGPIQQPSISHQHPPPRLINFQNHSPKPNGPVLPPYPQQLRYSPSQNVPRQTTIKPNPLQMAFLAQQAIKQWQISSVQAPPTTASSSSSTPSSPTITSAAGYDGKAFSSPMIDLSAPVGGSYNLPSLPDIDCSSTIMLDNIARKDTGVDHAQPRPPSNRTVQSPNSSVPSPGLAGPVTMTSVHPPIRSPSASSVGSRGSSGSSSKPAGADSTHKVPVVMLEPIRIKQENSGPPENYDFPVVIVKQESDEESRPQNTNYPRSILTSLLLNSSQSSASEETVLRSDAPDSTGDQPGLHQENSSNGKSEWSDASQKSPVHVGETRKEDDPNEDWCAVCQNGGELLCCEKCPKVFHLTCHVPTLTNFPSGEWICTFCRDLSKPEVDYDCDVPSHHSEKRKSEGLTKLTPIDKRKCERLLLFLYCHEMSLAFQDPVPLTVPDYYKIIKNPMDLSTIKKRLQEDYCMYTKPEDFVADFRLIFQNCAEFNEPDSEVANAGIKLESYFEELLKNLYPEKRFPKVEFRHEAEDCKFSDDSDDDFVQPRKKRLKSTEDRQLLK

Target ORF information:

RefSeq Version	XM_006505895.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tripartite motif-containing 24 (Trim24), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006505895.2

ORF Insert Sequence:

ATGGAAAAAA CAAAATATAT AAAGTATACA GGAAATCAGA TCCAAAATAG GATAATTGAA 
ATAAATCAAA ACCAAAAGCA GGTGGAACAG GATATTAAAG TTGCCATCTT CACATTGATG 
GTGGAGATAA ACAAAAAAGG GAAAGCTCTG CTGCACCAGC TTGAGAGTCT TGCAAAGGAC 
CATCGAATGA AACTCATGCA ACAACAGCAG GAAGTGGCTG GGCTTTCTAA GCAGTTAGAG 
CACGTCATGC ATTTTTCTAA ATGGGCTGTT TCCAGTGGCA GCAGCACAGC CTTGCTGTAC 
AGCAAGCGGC TGATTACATA CAGGTTACGG CACCTTCTTC GTGCAAGGTG TGATGCTTCT 
CCTGTGACCA ACACCACCAT CCAGTTTCAC TGTGATCCTA GTTTCTGGGC TCAAAATATT 
ATCAACTTGG GTTCTTTAGT AATCGAGGAT AAAGAGAGCC AGCCACAAAT GCCTAAGCAG 
AATCCTGTCG TGGAGCAGAG TTCACAGCCA CCAGGTGGTT TACCTTCCAA CCAGTTATCC 
AAGTTCCCAA CACAGATCAG CCTAGCTCAG TTACGACTCC AGCATATTCA GCAACAGGTA 
ATGGCTCAGA GGCAACAGGT GCAACGGAGG CCAGCACCTG TGGGTTTACC AAACCCTAGA 
ATGCAGGGGC CCATCCAGCA GCCTTCCATC TCTCATCAGC ATCCGCCACC ACGCTTAATA 
AACTTTCAGA ATCACAGCCC TAAGCCCAAT GGACCAGTTC TTCCTCCTTA TCCTCAGCAG 
CTGAGATATT CACCAAGCCA GAATGTACCT CGGCAGACAA CAATAAAGCC CAACCCCTTG 
CAAATGGCTT TTTTGGCTCA ACAGGCCATA AAACAGTGGC AGATCAGCAG TGTACAGGCT 
CCGCCCACAA CTGCCAGCAG CTCCTCCTCC ACGCCGTCCA GCCCCACAAT CACAAGTGCA 
GCTGGGTACG ATGGAAAAGC TTTTAGTTCA CCCATGATTG ATCTGAGTGC ACCGGTGGGA 
GGGTCTTACA ATCTTCCTTC TCTTCCAGAT ATTGATTGTT CAAGTACTAT AATGTTGGAC 
AACATTGCAA GGAAAGACAC AGGTGTAGAT CACGCCCAGC CGAGGCCTCC GTCAAACAGA 
ACGGTGCAGT CACCAAATTC ATCAGTGCCA TCTCCAGGCC TTGCAGGGCC TGTTACTATG 
ACTAGCGTCC ATCCCCCAAT ACGTTCACCT AGTGCCTCCA GTGTTGGAAG TCGAGGAAGC 
TCTGGCTCTT CCAGCAAACC AGCAGGAGCT GATTCTACTC ACAAGGTCCC AGTAGTCATG 
CTGGAGCCAA TTCGAATAAA ACAGGAAAAC AGTGGACCAC CTGAAAATTA TGATTTTCCT 
GTTGTTATAG TAAAACAAGA ATCAGATGAA GAATCTAGAC CTCAAAATAC TAACTATCCA 
AGAAGCATAC TTACCTCCCT CCTCTTAAAC AGCAGTCAGA GCTCTGCTTC TGAGGAAACC 
GTGTTACGAT CTGATGCCCC TGATAGTACA GGAGATCAGC CTGGACTCCA TCAAGAAAAT 
TCCTCAAATG GAAAGTCTGA GTGGTCGGAT GCCTCCCAGA AGTCCCCTGT GCATGTCGGA 
GAGACGAGGA AGGAGGATGA CCCCAATGAA GACTGGTGTG CTGTTTGTCA AAATGGTGGG 
GAACTCCTAT GCTGTGAGAA ATGTCCTAAA GTATTCCATC TTACTTGTCA TGTGCCCACC 
TTGACAAATT TTCCAAGTGG AGAATGGATC TGTACTTTCT GCCGAGACTT ATCTAAGCCA 
GAGGTTGACT ATGATTGTGA TGTTCCCAGT CACCACTCAG AGAAACGGAA AAGTGAAGGC 
CTTACTAAGT TAACGCCAAT AGACAAAAGG AAATGTGAAC GCCTACTTCT GTTTCTTTAC 
TGCCATGAAA TGAGCCTGGC TTTCCAAGAC CCTGTTCCTC TAACTGTGCC TGATTATTAT 
AAAATAATTA AAAACCCAAT GGACTTGTCA ACCATCAAGA AAAGACTTCA GGAGGATTAT 
TGCATGTATA CAAAGCCTGA AGACTTTGTA GCTGATTTTA GATTGATCTT TCAAAACTGT 
GCTGAATTCA ATGAGCCTGA TTCTGAAGTA GCCAATGCTG GTATAAAACT TGAAAGCTAT 
TTTGAAGAAC TTCTAAAGAA TCTTTATCCA GAAAAAAGGT TTCCTAAGGT AGAATTCAGG 
CATGAAGCAG AAGACTGTAA GTTCAGTGAC GACTCAGACG ATGACTTTGT ACAGCCCCGG 
AAGAAGCGTC TCAAGAGCAC CGAGGATCGC CAGCTGCTTA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trim24 ( NM_001272076.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001259005.1 Mus musculus tripartite motif-containing 24 (Trim24), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu17041
Clone ID Related Accession (Same CDS sequence)	NM_001272076.1
Accession Version	NM_001272076.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	transcription intermediary factor 1-alpha isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK133144.1, S78219.1 and AC113501.7. Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region, and uses an alternate start codon, compared to variant 1. The encoded isoform (3) has a distinct and shorter N-terminus that lacks the N-terminal RING domain, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK133144.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGATA TTTTTGATTA CACTTTTATC GGTGGGTGTG GAGATGCTTG TCCCACATGC 
GCATGTGGAA GTCAGAGGAT AACTTGGAAA AGTCAGTTCT TTCCATCCTG TGTTCGTCCC 
ATGGATAAAA CTGCTGTCAC TGGTGATGAG CAAGTTGGAG TCATTCGATG CCCAGTTTGC 
AGTCAAGAGT GTGCTGAGAG ACACATCATA GACAACTTTT TTGTGAAGGA CACCACTGAA 
GTTCCTAGTA GTACAGTAGA AAAGTCTAAT CAGGTATGTA CAAGCTGTGA AGACAATGCA 
GAAGCTAATG GGTTTTGTGT AGAGTGTGTT GAATGGCTCT GCAAGACATG TATTAGAGCT 
CACCAGAGGG TGAAGTTCAC AAAAGACCAC ACAGTCAGGC AGAAAGAAGA AGTATCTCCA 
GAGGCAGTTG GGGTGACCAG TCAGCGACCA GTGTTTTGTC CCTTCCATAA AAAGGAGCAG 
TTGAAACTTT ACTGTGAAAC ATGTGATAAA CTGACCTGTC GAGACTGCCA GCTGCTAGAA 
CACAAAGAAC ACAGGTATCA ATTTATAGAA GAAGCTTTTC AGAATCAAAA AGTGATCATA 
GATACTCTAA TCACCAAACT GATGGAAAAA ACAAAATATA TAAAGTATAC AGGAAATCAG 
ATCCAAAATA GGATAATTGA AATAAATCAA AACCAAAAGC AGGTGGAACA GGATATTAAA 
GTTGCCATCT TCACATTGAT GGTGGAGATA AACAAAAAAG GGAAAGCTCT GCTGCACCAG 
CTTGAGAGTC TTGCAAAGGA CCATCGAATG AAACTCATGC AACAACAGCA GGAAGTGGCT 
GGGCTTTCTA AGCAGTTAGA GCACGTCATG CATTTTTCTA AATGGGCTGT TTCCAGTGGC 
AGCAGCACAG CCTTGCTGTA CAGCAAGCGG CTGATTACAT ACAGGTTACG GCACCTTCTT 
CGTGCAAGGT GTGATGCTTC TCCTGTGACC AACACCACCA TCCAGTTTCA CTGTGATCCT 
AGTTTCTGGG CTCAAAATAT TATCAACTTG GGTTCTTTAG TAATCGAGGA TAAAGAGAGC 
CAGCCACAAA TGCCTAAGCA GAATCCTGTC GTGGAGCAGA GTTCACAGCC ACCAGGTGGT 
TTACCTTCCA ACCAGTTATC CAAGTTCCCA ACACAGATCA GCCTAGCTCA GTTACGACTC 
CAGCATATTC AGCAACAGGT AATGGCTCAG AGGCAACAGG TGCAACGGAG GCCAGCACCT 
GTGGGTTTAC CAAACCCTAG AATGCAGGGG CCCATCCAGC AGCCTTCCAT CTCTCATCAG 
CATCCGCCAC CACGCTTAAT AAACTTTCAG AATCACAGCC CTAAGCCCAA TGGACCAGTT 
CTTCCTCCTT ATCCTCAGCA GCTGAGATAT TCACCAAGCC AGAATGTACC TCGGCAGACA 
ACAATAAAGC CCAACCCCTT GCAAATGGCT TTTTTGGCTC AACAGGCCAT AAAACAGTGG 
CAGATCAGCA GTGTACAGGC TCCGCCCACA ACTGCCAGCA GCTCCTCCTC CACGCCGTCC 
AGCCCCACAA TCACAAGTGC AGCTGGGTAC GATGGAAAAG CTTTTAGTTC ACCCATGATT 
GATCTGAGTG CACCGGTGGG AGGGTCTTAC AATCTTCCTT CTCTTCCAGA TATTGATTGT 
TCAAGTACTA TAATGTTGGA CAACATTGCA AGGAAAGACA CAGGTGTAGA TCACGCCCAG 
CCGAGGCCTC CGTCAAACAG AACGGTGCAG TCACCAAATT CATCAGTGCC ATCTCCAGGC 
CTTGCAGGGC CTGTTACTAT GACTAGCGTC CATCCCCCAA TACGTTCACC TAGTGCCTCC 
AGTGTTGGAA GTCGAGGAAG CTCTGGCTCT TCCAGCAAAC CAGCAGGAGC TGATTCTACT 
CACAAGGTCC CAGTAGTCAT GCTGGAGCCA ATTCGAATAA AACAGGAAAA CAGTGGACCA 
CCTGAAAATT ATGATTTTCC TGTTGTTATA GTAAAACAAG AATCAGATGA AGAATCTAGA 
CCTCAAAATA CTAACTATCC AAGAAGCATA CTTACCTCCC TCCTCTTAAA CAGCAGTCAG 
AGCTCTGCTT CTGAGGAAAC CGTGTTACGA TCTGATGCCC CTGATAGTAC AGGAGATCAG 
CCTGGACTCC ATCAAGAAAA TTCCTCAAAT GGAAAGTCTG AGTGGTCGGA TGCCTCCCAG 
AAGTCCCCTG TGCATGTCGG AGAGACGAGG AAGGAGGATG ACCCCAATGA AGACTGGTGT 
GCTGTTTGTC AAAATGGTGG GGAACTCCTA TGCTGTGAGA AATGTCCTAA AGTATTCCAT 
CTTACTTGTC ATGTGCCCAC CTTGACAAAT TTTCCAAGTG GAGAATGGAT CTGTACTTTC 
TGCCGAGACT TATCTAAGCC AGAGGTTGAC TATGATTGTG ATGTTCCCAG TCACCACTCA 
GAGAAACGGA AAAGTGAAGG CCTTACTAAG TTAACGCCAA TAGACAAAAG GAAATGTGAA 
CGCCTACTTC TGTTTCTTTA CTGCCATGAA ATGAGCCTGG CTTTCCAAGA CCCTGTTCCT 
CTAACTGTGC CTGATTATTA TAAAATAATT AAAAACCCAA TGGACTTGTC AACCATCAAG 
AAAAGACTTC AGGAGGATTA TTGCATGTAT ACAAAGCCTG AAGACTTTGT AGCTGATTTT 
AGATTGATCT TTCAAAACTG TGCTGAATTC AATGAGCCTG ATTCTGAAGT AGCCAATGCT 
GGTATAAAAC TTGAAAGCTA TTTTGAAGAA CTTCTAAAGA ATCTTTATCC AGAAAAAAGG 
TTTCCTAAGG TAGAATTCAG GCATGAAGCA GAAGACTGTA AGTTCAGTGA CGACTCAGAC 
GATGACTTTG TACAGCCCCG GAAGAAGCGT CTCAAGAGCA CCGAGGATCG CCAGCTGCTT 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259005.1
CDS	91..3036
Translation	MADIFDYTFIGGCGDACPTCACGSQRITWKSQFFPSCVRPMDKTAVTGDEQVGVIRCPVCSQECAERHIIDNFFVKDTTEVPSSTVEKSNQVCTSCEDNAEANGFCVECVEWLCKTCIRAHQRVKFTKDHTVRQKEEVSPEAVGVTSQRPVFCPFHKKEQLKLYCETCDKLTCRDCQLLEHKEHRYQFIEEAFQNQKVIIDTLITKLMEKTKYIKYTGNQIQNRIIEINQNQKQVEQDIKVAIFTLMVEINKKGKALLHQLESLAKDHRMKLMQQQQEVAGLSKQLEHVMHFSKWAVSSGSSTALLYSKRLITYRLRHLLRARCDASPVTNTTIQFHCDPSFWAQNIINLGSLVIEDKESQPQMPKQNPVVEQSSQPPGGLPSNQLSKFPTQISLAQLRLQHIQQQVMAQRQQVQRRPAPVGLPNPRMQGPIQQPSISHQHPPPRLINFQNHSPKPNGPVLPPYPQQLRYSPSQNVPRQTTIKPNPLQMAFLAQQAIKQWQISSVQAPPTTASSSSSTPSSPTITSAAGYDGKAFSSPMIDLSAPVGGSYNLPSLPDIDCSSTIMLDNIARKDTGVDHAQPRPPSNRTVQSPNSSVPSPGLAGPVTMTSVHPPIRSPSASSVGSRGSSGSSSKPAGADSTHKVPVVMLEPIRIKQENSGPPENYDFPVVIVKQESDEESRPQNTNYPRSILTSLLLNSSQSSASEETVLRSDAPDSTGDQPGLHQENSSNGKSEWSDASQKSPVHVGETRKEDDPNEDWCAVCQNGGELLCCEKCPKVFHLTCHVPTLTNFPSGEWICTFCRDLSKPEVDYDCDVPSHHSEKRKSEGLTKLTPIDKRKCERLLLFLYCHEMSLAFQDPVPLTVPDYYKIIKNPMDLSTIKKRLQEDYCMYTKPEDFVADFRLIFQNCAEFNEPDSEVANAGIKLESYFEELLKNLYPEKRFPKVEFRHEAEDCKFSDDSDDDFVQPRKKRLKSTEDRQLLK

Target ORF information:

RefSeq Version	NM_001272076.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tripartite motif-containing 24 (Trim24), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272076.1

ORF Insert Sequence:

ATGGCAGATA TTTTTGATTA CACTTTTATC GGTGGGTGTG GAGATGCTTG TCCCACATGC 
GCATGTGGAA GTCAGAGGAT AACTTGGAAA AGTCAGTTCT TTCCATCCTG TGTTCGTCCC 
ATGGATAAAA CTGCTGTCAC TGGTGATGAG CAAGTTGGAG TCATTCGATG CCCAGTTTGC 
AGTCAAGAGT GTGCTGAGAG ACACATCATA GACAACTTTT TTGTGAAGGA CACCACTGAA 
GTTCCTAGTA GTACAGTAGA AAAGTCTAAT CAGGTATGTA CAAGCTGTGA AGACAATGCA 
GAAGCTAATG GGTTTTGTGT AGAGTGTGTT GAATGGCTCT GCAAGACATG TATTAGAGCT 
CACCAGAGGG TGAAGTTCAC AAAAGACCAC ACAGTCAGGC AGAAAGAAGA AGTATCTCCA 
GAGGCAGTTG GGGTGACCAG TCAGCGACCA GTGTTTTGTC CCTTCCATAA AAAGGAGCAG 
TTGAAACTTT ACTGTGAAAC ATGTGATAAA CTGACCTGTC GAGACTGCCA GCTGCTAGAA 
CACAAAGAAC ACAGGTATCA ATTTATAGAA GAAGCTTTTC AGAATCAAAA AGTGATCATA 
GATACTCTAA TCACCAAACT GATGGAAAAA ACAAAATATA TAAAGTATAC AGGAAATCAG 
ATCCAAAATA GGATAATTGA AATAAATCAA AACCAAAAGC AGGTGGAACA GGATATTAAA 
GTTGCCATCT TCACATTGAT GGTGGAGATA AACAAAAAAG GGAAAGCTCT GCTGCACCAG 
CTTGAGAGTC TTGCAAAGGA CCATCGAATG AAACTCATGC AACAACAGCA GGAAGTGGCT 
GGGCTTTCTA AGCAGTTAGA GCACGTCATG CATTTTTCTA AATGGGCTGT TTCCAGTGGC 
AGCAGCACAG CCTTGCTGTA CAGCAAGCGG CTGATTACAT ACAGGTTACG GCACCTTCTT 
CGTGCAAGGT GTGATGCTTC TCCTGTGACC AACACCACCA TCCAGTTTCA CTGTGATCCT 
AGTTTCTGGG CTCAAAATAT TATCAACTTG GGTTCTTTAG TAATCGAGGA TAAAGAGAGC 
CAGCCACAAA TGCCTAAGCA GAATCCTGTC GTGGAGCAGA GTTCACAGCC ACCAGGTGGT 
TTACCTTCCA ACCAGTTATC CAAGTTCCCA ACACAGATCA GCCTAGCTCA GTTACGACTC 
CAGCATATTC AGCAACAGGT AATGGCTCAG AGGCAACAGG TGCAACGGAG GCCAGCACCT 
GTGGGTTTAC CAAACCCTAG AATGCAGGGG CCCATCCAGC AGCCTTCCAT CTCTCATCAG 
CATCCGCCAC CACGCTTAAT AAACTTTCAG AATCACAGCC CTAAGCCCAA TGGACCAGTT 
CTTCCTCCTT ATCCTCAGCA GCTGAGATAT TCACCAAGCC AGAATGTACC TCGGCAGACA 
ACAATAAAGC CCAACCCCTT GCAAATGGCT TTTTTGGCTC AACAGGCCAT AAAACAGTGG 
CAGATCAGCA GTGTACAGGC TCCGCCCACA ACTGCCAGCA GCTCCTCCTC CACGCCGTCC 
AGCCCCACAA TCACAAGTGC AGCTGGGTAC GATGGAAAAG CTTTTAGTTC ACCCATGATT 
GATCTGAGTG CACCGGTGGG AGGGTCTTAC AATCTTCCTT CTCTTCCAGA TATTGATTGT 
TCAAGTACTA TAATGTTGGA CAACATTGCA AGGAAAGACA CAGGTGTAGA TCACGCCCAG 
CCGAGGCCTC CGTCAAACAG AACGGTGCAG TCACCAAATT CATCAGTGCC ATCTCCAGGC 
CTTGCAGGGC CTGTTACTAT GACTAGCGTC CATCCCCCAA TACGTTCACC TAGTGCCTCC 
AGTGTTGGAA GTCGAGGAAG CTCTGGCTCT TCCAGCAAAC CAGCAGGAGC TGATTCTACT 
CACAAGGTCC CAGTAGTCAT GCTGGAGCCA ATTCGAATAA AACAGGAAAA CAGTGGACCA 
CCTGAAAATT ATGATTTTCC TGTTGTTATA GTAAAACAAG AATCAGATGA AGAATCTAGA 
CCTCAAAATA CTAACTATCC AAGAAGCATA CTTACCTCCC TCCTCTTAAA CAGCAGTCAG 
AGCTCTGCTT CTGAGGAAAC CGTGTTACGA TCTGATGCCC CTGATAGTAC AGGAGATCAG 
CCTGGACTCC ATCAAGAAAA TTCCTCAAAT GGAAAGTCTG AGTGGTCGGA TGCCTCCCAG 
AAGTCCCCTG TGCATGTCGG AGAGACGAGG AAGGAGGATG ACCCCAATGA AGACTGGTGT 
GCTGTTTGTC AAAATGGTGG GGAACTCCTA TGCTGTGAGA AATGTCCTAA AGTATTCCAT 
CTTACTTGTC ATGTGCCCAC CTTGACAAAT TTTCCAAGTG GAGAATGGAT CTGTACTTTC 
TGCCGAGACT TATCTAAGCC AGAGGTTGAC TATGATTGTG ATGTTCCCAG TCACCACTCA 
GAGAAACGGA AAAGTGAAGG CCTTACTAAG TTAACGCCAA TAGACAAAAG GAAATGTGAA 
CGCCTACTTC TGTTTCTTTA CTGCCATGAA ATGAGCCTGG CTTTCCAAGA CCCTGTTCCT 
CTAACTGTGC CTGATTATTA TAAAATAATT AAAAACCCAA TGGACTTGTC AACCATCAAG 
AAAAGACTTC AGGAGGATTA TTGCATGTAT ACAAAGCCTG AAGACTTTGT AGCTGATTTT 
AGATTGATCT TTCAAAACTG TGCTGAATTC AATGAGCCTG ATTCTGAAGT AGCCAATGCT 
GGTATAAAAC TTGAAAGCTA TTTTGAAGAA CTTCTAAAGA ATCTTTATCC AGAAAAAAGG 
TTTCCTAAGG TAGAATTCAG GCATGAAGCA GAAGACTGTA AGTTCAGTGA CGACTCAGAC 
GATGACTTTG TACAGCCCCG GAAGAAGCGT CTCAAGAGCA CCGAGGATCG CCAGCTGCTT 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trim24 ( XM_006505895.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006505958.1 Mus musculus tripartite motif-containing 24 (Trim24), transcript variant X1, mRNA.

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CloneID	OMu37118
Clone ID Related Accession (Same CDS sequence)	XM_006505895.2 , XM_006505895.3
Accession Version	XM_006505895.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	transcription intermediary factor 1-alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006505895.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAAA CAAAATATAT AAAGTATACA GGAAATCAGA TCCAAAATAG GATAATTGAA 
ATAAATCAAA ACCAAAAGCA GGTGGAACAG GATATTAAAG TTGCCATCTT CACATTGATG 
GTGGAGATAA ACAAAAAAGG GAAAGCTCTG CTGCACCAGC TTGAGAGTCT TGCAAAGGAC 
CATCGAATGA AACTCATGCA ACAACAGCAG GAAGTGGCTG GGCTTTCTAA GCAGTTAGAG 
CACGTCATGC ATTTTTCTAA ATGGGCTGTT TCCAGTGGCA GCAGCACAGC CTTGCTGTAC 
AGCAAGCGGC TGATTACATA CAGGTTACGG CACCTTCTTC GTGCAAGGTG TGATGCTTCT 
CCTGTGACCA ACACCACCAT CCAGTTTCAC TGTGATCCTA GTTTCTGGGC TCAAAATATT 
ATCAACTTGG GTTCTTTAGT AATCGAGGAT AAAGAGAGCC AGCCACAAAT GCCTAAGCAG 
AATCCTGTCG TGGAGCAGAG TTCACAGCCA CCAGGTGGTT TACCTTCCAA CCAGTTATCC 
AAGTTCCCAA CACAGATCAG CCTAGCTCAG TTACGACTCC AGCATATTCA GCAACAGGTA 
ATGGCTCAGA GGCAACAGGT GCAACGGAGG CCAGCACCTG TGGGTTTACC AAACCCTAGA 
ATGCAGGGGC CCATCCAGCA GCCTTCCATC TCTCATCAGC ATCCGCCACC ACGCTTAATA 
AACTTTCAGA ATCACAGCCC TAAGCCCAAT GGACCAGTTC TTCCTCCTTA TCCTCAGCAG 
CTGAGATATT CACCAAGCCA GAATGTACCT CGGCAGACAA CAATAAAGCC CAACCCCTTG 
CAAATGGCTT TTTTGGCTCA ACAGGCCATA AAACAGTGGC AGATCAGCAG TGTACAGGCT 
CCGCCCACAA CTGCCAGCAG CTCCTCCTCC ACGCCGTCCA GCCCCACAAT CACAAGTGCA 
GCTGGGTACG ATGGAAAAGC TTTTAGTTCA CCCATGATTG ATCTGAGTGC ACCGGTGGGA 
GGGTCTTACA ATCTTCCTTC TCTTCCAGAT ATTGATTGTT CAAGTACTAT AATGTTGGAC 
AACATTGCAA GGAAAGACAC AGGTGTAGAT CACGCCCAGC CGAGGCCTCC GTCAAACAGA 
ACGGTGCAGT CACCAAATTC ATCAGTGCCA TCTCCAGGCC TTGCAGGGCC TGTTACTATG 
ACTAGCGTCC ATCCCCCAAT ACGTTCACCT AGTGCCTCCA GTGTTGGAAG TCGAGGAAGC 
TCTGGCTCTT CCAGCAAACC AGCAGGAGCT GATTCTACTC ACAAGGTCCC AGTAGTCATG 
CTGGAGCCAA TTCGAATAAA ACAGGAAAAC AGTGGACCAC CTGAAAATTA TGATTTTCCT 
GTTGTTATAG TAAAACAAGA ATCAGATGAA GAATCTAGAC CTCAAAATAC TAACTATCCA 
AGAAGCATAC TTACCTCCCT CCTCTTAAAC AGCAGTCAGA GCTCTGCTTC TGAGGAAACC 
GTGTTACGAT CTGATGCCCC TGATAGTACA GGAGATCAGC CTGGACTCCA TCAAGAAAAT 
TCCTCAAATG GAAAGTCTGA GTGGTCGGAT GCCTCCCAGA AGTCCCCTGT GCATGTCGGA 
GAGACGAGGA AGGAGGATGA CCCCAATGAA GACTGGTGTG CTGTTTGTCA AAATGGTGGG 
GAACTCCTAT GCTGTGAGAA ATGTCCTAAA GTATTCCATC TTACTTGTCA TGTGCCCACC 
TTGACAAATT TTCCAAGTGG AGAATGGATC TGTACTTTCT GCCGAGACTT ATCTAAGCCA 
GAGGTTGACT ATGATTGTGA TGTTCCCAGT CACCACTCAG AGAAACGGAA AAGTGAAGGC 
CTTACTAAGT TAACGCCAAT AGACAAAAGG AAATGTGAAC GCCTACTTCT GTTTCTTTAC 
TGCCATGAAA TGAGCCTGGC TTTCCAAGAC CCTGTTCCTC TAACTGTGCC TGATTATTAT 
AAAATAATTA AAAACCCAAT GGACTTGTCA ACCATCAAGA AAAGACTTCA GGAGGATTAT 
TGCATGTATA CAAAGCCTGA AGACTTTGTA GCTGATTTTA GATTGATCTT TCAAAACTGT 
GCTGAATTCA ATGAGCCTGA TTCTGAAGTA GCCAATGCTG GTATAAAACT TGAAAGCTAT 
TTTGAAGAAC TTCTAAAGAA TCTTTATCCA GAAAAAAGGT TTCCTAAGGT AGAATTCAGG 
CATGAAGCAG AAGACTGTAA GTTCAGTGAC GACTCAGACG ATGACTTTGT ACAGCCCCGG 
AAGAAGCGTC TCAAGAGCAC CGAGGATCGC CAGCTGCTTA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006505958.1
CDS	984..3308
Translation	MEKTKYIKYTGNQIQNRIIEINQNQKQVEQDIKVAIFTLMVEINKKGKALLHQLESLAKDHRMKLMQQQQEVAGLSKQLEHVMHFSKWAVSSGSSTALLYSKRLITYRLRHLLRARCDASPVTNTTIQFHCDPSFWAQNIINLGSLVIEDKESQPQMPKQNPVVEQSSQPPGGLPSNQLSKFPTQISLAQLRLQHIQQQVMAQRQQVQRRPAPVGLPNPRMQGPIQQPSISHQHPPPRLINFQNHSPKPNGPVLPPYPQQLRYSPSQNVPRQTTIKPNPLQMAFLAQQAIKQWQISSVQAPPTTASSSSSTPSSPTITSAAGYDGKAFSSPMIDLSAPVGGSYNLPSLPDIDCSSTIMLDNIARKDTGVDHAQPRPPSNRTVQSPNSSVPSPGLAGPVTMTSVHPPIRSPSASSVGSRGSSGSSSKPAGADSTHKVPVVMLEPIRIKQENSGPPENYDFPVVIVKQESDEESRPQNTNYPRSILTSLLLNSSQSSASEETVLRSDAPDSTGDQPGLHQENSSNGKSEWSDASQKSPVHVGETRKEDDPNEDWCAVCQNGGELLCCEKCPKVFHLTCHVPTLTNFPSGEWICTFCRDLSKPEVDYDCDVPSHHSEKRKSEGLTKLTPIDKRKCERLLLFLYCHEMSLAFQDPVPLTVPDYYKIIKNPMDLSTIKKRLQEDYCMYTKPEDFVADFRLIFQNCAEFNEPDSEVANAGIKLESYFEELLKNLYPEKRFPKVEFRHEAEDCKFSDDSDDDFVQPRKKRLKSTEDRQLLK

Target ORF information:

RefSeq Version	XM_006505895.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tripartite motif-containing 24 (Trim24), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006505895.3

ORF Insert Sequence:

ATGGAAAAAA CAAAATATAT AAAGTATACA GGAAATCAGA TCCAAAATAG GATAATTGAA 
ATAAATCAAA ACCAAAAGCA GGTGGAACAG GATATTAAAG TTGCCATCTT CACATTGATG 
GTGGAGATAA ACAAAAAAGG GAAAGCTCTG CTGCACCAGC TTGAGAGTCT TGCAAAGGAC 
CATCGAATGA AACTCATGCA ACAACAGCAG GAAGTGGCTG GGCTTTCTAA GCAGTTAGAG 
CACGTCATGC ATTTTTCTAA ATGGGCTGTT TCCAGTGGCA GCAGCACAGC CTTGCTGTAC 
AGCAAGCGGC TGATTACATA CAGGTTACGG CACCTTCTTC GTGCAAGGTG TGATGCTTCT 
CCTGTGACCA ACACCACCAT CCAGTTTCAC TGTGATCCTA GTTTCTGGGC TCAAAATATT 
ATCAACTTGG GTTCTTTAGT AATCGAGGAT AAAGAGAGCC AGCCACAAAT GCCTAAGCAG 
AATCCTGTCG TGGAGCAGAG TTCACAGCCA CCAGGTGGTT TACCTTCCAA CCAGTTATCC 
AAGTTCCCAA CACAGATCAG CCTAGCTCAG TTACGACTCC AGCATATTCA GCAACAGGTA 
ATGGCTCAGA GGCAACAGGT GCAACGGAGG CCAGCACCTG TGGGTTTACC AAACCCTAGA 
ATGCAGGGGC CCATCCAGCA GCCTTCCATC TCTCATCAGC ATCCGCCACC ACGCTTAATA 
AACTTTCAGA ATCACAGCCC TAAGCCCAAT GGACCAGTTC TTCCTCCTTA TCCTCAGCAG 
CTGAGATATT CACCAAGCCA GAATGTACCT CGGCAGACAA CAATAAAGCC CAACCCCTTG 
CAAATGGCTT TTTTGGCTCA ACAGGCCATA AAACAGTGGC AGATCAGCAG TGTACAGGCT 
CCGCCCACAA CTGCCAGCAG CTCCTCCTCC ACGCCGTCCA GCCCCACAAT CACAAGTGCA 
GCTGGGTACG ATGGAAAAGC TTTTAGTTCA CCCATGATTG ATCTGAGTGC ACCGGTGGGA 
GGGTCTTACA ATCTTCCTTC TCTTCCAGAT ATTGATTGTT CAAGTACTAT AATGTTGGAC 
AACATTGCAA GGAAAGACAC AGGTGTAGAT CACGCCCAGC CGAGGCCTCC GTCAAACAGA 
ACGGTGCAGT CACCAAATTC ATCAGTGCCA TCTCCAGGCC TTGCAGGGCC TGTTACTATG 
ACTAGCGTCC ATCCCCCAAT ACGTTCACCT AGTGCCTCCA GTGTTGGAAG TCGAGGAAGC 
TCTGGCTCTT CCAGCAAACC AGCAGGAGCT GATTCTACTC ACAAGGTCCC AGTAGTCATG 
CTGGAGCCAA TTCGAATAAA ACAGGAAAAC AGTGGACCAC CTGAAAATTA TGATTTTCCT 
GTTGTTATAG TAAAACAAGA ATCAGATGAA GAATCTAGAC CTCAAAATAC TAACTATCCA 
AGAAGCATAC TTACCTCCCT CCTCTTAAAC AGCAGTCAGA GCTCTGCTTC TGAGGAAACC 
GTGTTACGAT CTGATGCCCC TGATAGTACA GGAGATCAGC CTGGACTCCA TCAAGAAAAT 
TCCTCAAATG GAAAGTCTGA GTGGTCGGAT GCCTCCCAGA AGTCCCCTGT GCATGTCGGA 
GAGACGAGGA AGGAGGATGA CCCCAATGAA GACTGGTGTG CTGTTTGTCA AAATGGTGGG 
GAACTCCTAT GCTGTGAGAA ATGTCCTAAA GTATTCCATC TTACTTGTCA TGTGCCCACC 
TTGACAAATT TTCCAAGTGG AGAATGGATC TGTACTTTCT GCCGAGACTT ATCTAAGCCA 
GAGGTTGACT ATGATTGTGA TGTTCCCAGT CACCACTCAG AGAAACGGAA AAGTGAAGGC 
CTTACTAAGT TAACGCCAAT AGACAAAAGG AAATGTGAAC GCCTACTTCT GTTTCTTTAC 
TGCCATGAAA TGAGCCTGGC TTTCCAAGAC CCTGTTCCTC TAACTGTGCC TGATTATTAT 
AAAATAATTA AAAACCCAAT GGACTTGTCA ACCATCAAGA AAAGACTTCA GGAGGATTAT 
TGCATGTATA CAAAGCCTGA AGACTTTGTA GCTGATTTTA GATTGATCTT TCAAAACTGT 
GCTGAATTCA ATGAGCCTGA TTCTGAAGTA GCCAATGCTG GTATAAAACT TGAAAGCTAT 
TTTGAAGAAC TTCTAAAGAA TCTTTATCCA GAAAAAAGGT TTCCTAAGGT AGAATTCAGG 
CATGAAGCAG AAGACTGTAA GTTCAGTGAC GACTCAGACG ATGACTTTGT ACAGCCCCGG 
AAGAAGCGTC TCAAGAGCAC CGAGGATCGC CAGCTGCTTA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trim24 ( NM_145076.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_659542.3 Mus musculus tripartite motif-containing 24 (Trim24), transcript variant 1, mRNA.

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CloneID	OMu12128
Clone ID Related Accession (Same CDS sequence)	NM_145076.4
Accession Version	NM_145076.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	transcription intermediary factor 1-alpha isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from S78221.1 and AC113501.7. On Jan 10, 2013 this sequence version replaced NM_145076.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC056959.1, AB221637.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGTGG CTGTGGAGAA GGCGGCGGCG GCAGCGGCTC CGGCCGGAGG CCCCGCAGCG 
GCGGCGCCGA GCGGGGAGAA TGAGGCCGAG AGCCGGCAGG GCCCGGACTC GGAGAGCGGC 
GGCGAGGCGT CCCGGCTCAA CCTGTTGGAC ACTTGCGCCG TGTGCCACCA GAACATCCAG 
AGCCGGGTGC CCAAGCTGCT GCCCTGCCTG CACTCGTTCT GCCAGCGCTG TTTGCCCGCG 
CCGCAGCGCT ATCTCATGCT GACGGCGCCC GCGCTGGGCT CGGCAGAGAC CCCTCCACCC 
GCTCCCGCCC CCGCCCCCGC CCCGGGCTCC CCGGCCGGTG GTCCTTCGCC ATTCGCCACC 
CAAGTTGGAG TCATTCGATG CCCAGTTTGC AGTCAAGAGT GTGCTGAGAG ACACATCATA 
GACAACTTTT TTGTGAAGGA CACCACTGAA GTTCCTAGTA GTACAGTAGA AAAGTCTAAT 
CAGGTATGTA CAAGCTGTGA AGACAATGCA GAAGCTAATG GGTTTTGTGT AGAGTGTGTT 
GAATGGCTCT GCAAGACATG TATTAGAGCT CACCAGAGGG TGAAGTTCAC AAAAGACCAC 
ACAGTCAGGC AGAAAGAAGA AGTATCTCCA GAGGCAGTTG GGGTGACCAG TCAGCGACCA 
GTGTTTTGTC CCTTCCATAA AAAGGAGCAG TTGAAACTTT ACTGTGAAAC ATGTGATAAA 
CTGACCTGTC GAGACTGCCA GCTGCTAGAA CACAAAGAAC ACAGGTATCA ATTTATAGAA 
GAAGCTTTTC AGAATCAAAA AGTGATCATA GATACTCTAA TCACCAAACT GATGGAAAAA 
ACAAAATATA TAAAGTATAC AGGAAATCAG ATCCAAAATA GGATAATTGA AATAAATCAA 
AACCAAAAGC AGGTGGAACA GGATATTAAA GTTGCCATCT TCACATTGAT GGTGGAGATA 
AACAAAAAAG GGAAAGCTCT GCTGCACCAG CTTGAGAGTC TTGCAAAGGA CCATCGAATG 
AAACTCATGC AACAACAGCA GGAAGTGGCT GGGCTTTCTA AGCAGTTAGA GCACGTCATG 
CATTTTTCTA AATGGGCTGT TTCCAGTGGC AGCAGCACAG CCTTGCTGTA CAGCAAGCGG 
CTGATTACAT ACAGGTTACG GCACCTTCTT CGTGCAAGGT GTGATGCTTC TCCTGTGACC 
AACACCACCA TCCAGTTTCA CTGTGATCCT AGTTTCTGGG CTCAAAATAT TATCAACTTG 
GGTTCTTTAG TAATCGAGGA TAAAGAGAGC CAGCCACAAA TGCCTAAGCA GAATCCTGTC 
GTGGAGCAGA GTTCACAGCC ACCAGGTGGT TTACCTTCCA ACCAGTTATC CAAGTTCCCA 
ACACAGATCA GCCTAGCTCA GTTACGACTC CAGCATATTC AGCAACAGGT AATGGCTCAG 
AGGCAACAGG TGCAACGGAG GCCAGCACCT GTGGGTTTAC CAAACCCTAG AATGCAGGGG 
CCCATCCAGC AGCCTTCCAT CTCTCATCAG CATCCGCCAC CACGCTTAAT AAACTTTCAG 
AATCACAGCC CTAAGCCCAA TGGACCAGTT CTTCCTCCTT ATCCTCAGCA GCTGAGATAT 
TCACCAAGCC AGAATGTACC TCGGCAGACA ACAATAAAGC CCAACCCCTT GCAAATGGCT 
TTTTTGGCTC AACAGGCCAT AAAACAGTGG CAGATCAGCA GTGTACAGGC TCCGCCCACA 
ACTGCCAGCA GCTCCTCCTC CACGCCGTCC AGCCCCACAA TCACAAGTGC AGCTGGGTAC 
GATGGAAAAG CTTTTAGTTC ACCCATGATT GATCTGAGTG CACCGGTGGG AGGGTCTTAC 
AATCTTCCTT CTCTTCCAGA TATTGATTGT TCAAGTACTA TAATGTTGGA CAACATTGCA 
AGGAAAGACA CAGGTGTAGA TCACGCCCAG CCGAGGCCTC CGTCAAACAG AACGGTGCAG 
TCACCAAATT CATCAGTGCC ATCTCCAGGC CTTGCAGGGC CTGTTACTAT GACTAGCGTC 
CATCCCCCAA TACGTTCACC TAGTGCCTCC AGTGTTGGAA GTCGAGGAAG CTCTGGCTCT 
TCCAGCAAAC CAGCAGGAGC TGATTCTACT CACAAGGTCC CAGTAGTCAT GCTGGAGCCA 
ATTCGAATAA AACAGGAAAA CAGTGGACCA CCTGAAAATT ATGATTTTCC TGTTGTTATA 
GTAAAACAAG AATCAGATGA AGAATCTAGA CCTCAAAATA CTAACTATCC AAGAAGCATA 
CTTACCTCCC TCCTCTTAAA CAGCAGTCAG AGCTCTGCTT CTGAGGAAAC CGTGTTACGA 
TCTGATGCCC CTGATAGTAC AGGAGATCAG CCTGGACTCC ATCAAGAAAA TTCCTCAAAT 
GGAAAGTCTG AGTGGTCGGA TGCCTCCCAG AAGTCCCCTG TGCATGTCGG AGAGACGAGG 
AAGGAGGATG ACCCCAATGA AGACTGGTGT GCTGTTTGTC AAAATGGTGG GGAACTCCTA 
TGCTGTGAGA AATGTCCTAA AGTATTCCAT CTTACTTGTC ATGTGCCCAC CTTGACAAAT 
TTTCCAAGTG GAGAATGGAT CTGTACTTTC TGCCGAGACT TATCTAAGCC AGAGGTTGAC 
TATGATTGTG ATGTTCCCAG TCACCACTCA GAGAAACGGA AAAGTGAAGG CCTTACTAAG 
TTAACGCCAA TAGACAAAAG GAAATGTGAA CGCCTACTTC TGTTTCTTTA CTGCCATGAA 
ATGAGCCTGG CTTTCCAAGA CCCTGTTCCT CTAACTGTGC CTGATTATTA TAAAATAATT 
AAAAACCCAA TGGACTTGTC AACCATCAAG AAAAGACTTC AGGAGGATTA TTGCATGTAT 
ACAAAGCCTG AAGACTTTGT AGCTGATTTT AGATTGATCT TTCAAAACTG TGCTGAATTC 
AATGAGCCTG ATTCTGAAGT AGCCAATGCT GGTATAAAAC TTGAAAGCTA TTTTGAAGAA 
CTTCTAAAGA ATCTTTATCC AGAAAAAAGG TTTCCTAAGG TAGAATTCAG GCATGAAGCA 
GAAGACTGTA AGTTCAGTGA CGACTCAGAC GATGACTTTG TACAGCCCCG GAAGAAGCGT 
CTCAAGAGCA CCGAGGATCG CCAGCTGCTT AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_659542.3
CDS	301..3456
Translation	MEVAVEKAAAAAAPAGGPAAAAPSGENEAESRQGPDSESGGEASRLNLLDTCAVCHQNIQSRVPKLLPCLHSFCQRCLPAPQRYLMLTAPALGSAETPPPAPAPAPAPGSPAGGPSPFATQVGVIRCPVCSQECAERHIIDNFFVKDTTEVPSSTVEKSNQVCTSCEDNAEANGFCVECVEWLCKTCIRAHQRVKFTKDHTVRQKEEVSPEAVGVTSQRPVFCPFHKKEQLKLYCETCDKLTCRDCQLLEHKEHRYQFIEEAFQNQKVIIDTLITKLMEKTKYIKYTGNQIQNRIIEINQNQKQVEQDIKVAIFTLMVEINKKGKALLHQLESLAKDHRMKLMQQQQEVAGLSKQLEHVMHFSKWAVSSGSSTALLYSKRLITYRLRHLLRARCDASPVTNTTIQFHCDPSFWAQNIINLGSLVIEDKESQPQMPKQNPVVEQSSQPPGGLPSNQLSKFPTQISLAQLRLQHIQQQVMAQRQQVQRRPAPVGLPNPRMQGPIQQPSISHQHPPPRLINFQNHSPKPNGPVLPPYPQQLRYSPSQNVPRQTTIKPNPLQMAFLAQQAIKQWQISSVQAPPTTASSSSSTPSSPTITSAAGYDGKAFSSPMIDLSAPVGGSYNLPSLPDIDCSSTIMLDNIARKDTGVDHAQPRPPSNRTVQSPNSSVPSPGLAGPVTMTSVHPPIRSPSASSVGSRGSSGSSSKPAGADSTHKVPVVMLEPIRIKQENSGPPENYDFPVVIVKQESDEESRPQNTNYPRSILTSLLLNSSQSSASEETVLRSDAPDSTGDQPGLHQENSSNGKSEWSDASQKSPVHVGETRKEDDPNEDWCAVCQNGGELLCCEKCPKVFHLTCHVPTLTNFPSGEWICTFCRDLSKPEVDYDCDVPSHHSEKRKSEGLTKLTPIDKRKCERLLLFLYCHEMSLAFQDPVPLTVPDYYKIIKNPMDLSTIKKRLQEDYCMYTKPEDFVADFRLIFQNCAEFNEPDSEVANAGIKLESYFEELLKNLYPEKRFPKVEFRHEAEDCKFSDDSDDDFVQPRKKRLKSTEDRQLLK

Target ORF information:

RefSeq Version	NM_145076.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus tripartite motif-containing 24 (Trim24), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145076.4

ORF Insert Sequence:

ATGGAGGTGG CTGTGGAGAA GGCGGCGGCG GCAGCGGCTC CGGCCGGAGG CCCCGCAGCG 
GCGGCGCCGA GCGGGGAGAA TGAGGCCGAG AGCCGGCAGG GCCCGGACTC GGAGAGCGGC 
GGCGAGGCGT CCCGGCTCAA CCTGTTGGAC ACTTGCGCCG TGTGCCACCA GAACATCCAG 
AGCCGGGTGC CCAAGCTGCT GCCCTGCCTG CACTCGTTCT GCCAGCGCTG TTTGCCCGCG 
CCGCAGCGCT ATCTCATGCT GACGGCGCCC GCGCTGGGCT CGGCAGAGAC CCCTCCACCC 
GCTCCCGCCC CCGCCCCCGC CCCGGGCTCC CCGGCCGGTG GTCCTTCGCC ATTCGCCACC 
CAAGTTGGAG TCATTCGATG CCCAGTTTGC AGTCAAGAGT GTGCTGAGAG ACACATCATA 
GACAACTTTT TTGTGAAGGA CACCACTGAA GTTCCTAGTA GTACAGTAGA AAAGTCTAAT 
CAGGTATGTA CAAGCTGTGA AGACAATGCA GAAGCTAATG GGTTTTGTGT AGAGTGTGTT 
GAATGGCTCT GCAAGACATG TATTAGAGCT CACCAGAGGG TGAAGTTCAC AAAAGACCAC 
ACAGTCAGGC AGAAAGAAGA AGTATCTCCA GAGGCAGTTG GGGTGACCAG TCAGCGACCA 
GTGTTTTGTC CCTTCCATAA AAAGGAGCAG TTGAAACTTT ACTGTGAAAC ATGTGATAAA 
CTGACCTGTC GAGACTGCCA GCTGCTAGAA CACAAAGAAC ACAGGTATCA ATTTATAGAA 
GAAGCTTTTC AGAATCAAAA AGTGATCATA GATACTCTAA TCACCAAACT GATGGAAAAA 
ACAAAATATA TAAAGTATAC AGGAAATCAG ATCCAAAATA GGATAATTGA AATAAATCAA 
AACCAAAAGC AGGTGGAACA GGATATTAAA GTTGCCATCT TCACATTGAT GGTGGAGATA 
AACAAAAAAG GGAAAGCTCT GCTGCACCAG CTTGAGAGTC TTGCAAAGGA CCATCGAATG 
AAACTCATGC AACAACAGCA GGAAGTGGCT GGGCTTTCTA AGCAGTTAGA GCACGTCATG 
CATTTTTCTA AATGGGCTGT TTCCAGTGGC AGCAGCACAG CCTTGCTGTA CAGCAAGCGG 
CTGATTACAT ACAGGTTACG GCACCTTCTT CGTGCAAGGT GTGATGCTTC TCCTGTGACC 
AACACCACCA TCCAGTTTCA CTGTGATCCT AGTTTCTGGG CTCAAAATAT TATCAACTTG 
GGTTCTTTAG TAATCGAGGA TAAAGAGAGC CAGCCACAAA TGCCTAAGCA GAATCCTGTC 
GTGGAGCAGA GTTCACAGCC ACCAGGTGGT TTACCTTCCA ACCAGTTATC CAAGTTCCCA 
ACACAGATCA GCCTAGCTCA GTTACGACTC CAGCATATTC AGCAACAGGT AATGGCTCAG 
AGGCAACAGG TGCAACGGAG GCCAGCACCT GTGGGTTTAC CAAACCCTAG AATGCAGGGG 
CCCATCCAGC AGCCTTCCAT CTCTCATCAG CATCCGCCAC CACGCTTAAT AAACTTTCAG 
AATCACAGCC CTAAGCCCAA TGGACCAGTT CTTCCTCCTT ATCCTCAGCA GCTGAGATAT 
TCACCAAGCC AGAATGTACC TCGGCAGACA ACAATAAAGC CCAACCCCTT GCAAATGGCT 
TTTTTGGCTC AACAGGCCAT AAAACAGTGG CAGATCAGCA GTGTACAGGC TCCGCCCACA 
ACTGCCAGCA GCTCCTCCTC CACGCCGTCC AGCCCCACAA TCACAAGTGC AGCTGGGTAC 
GATGGAAAAG CTTTTAGTTC ACCCATGATT GATCTGAGTG CACCGGTGGG AGGGTCTTAC 
AATCTTCCTT CTCTTCCAGA TATTGATTGT TCAAGTACTA TAATGTTGGA CAACATTGCA 
AGGAAAGACA CAGGTGTAGA TCACGCCCAG CCGAGGCCTC CGTCAAACAG AACGGTGCAG 
TCACCAAATT CATCAGTGCC ATCTCCAGGC CTTGCAGGGC CTGTTACTAT GACTAGCGTC 
CATCCCCCAA TACGTTCACC TAGTGCCTCC AGTGTTGGAA GTCGAGGAAG CTCTGGCTCT 
TCCAGCAAAC CAGCAGGAGC TGATTCTACT CACAAGGTCC CAGTAGTCAT GCTGGAGCCA 
ATTCGAATAA AACAGGAAAA CAGTGGACCA CCTGAAAATT ATGATTTTCC TGTTGTTATA 
GTAAAACAAG AATCAGATGA AGAATCTAGA CCTCAAAATA CTAACTATCC AAGAAGCATA 
CTTACCTCCC TCCTCTTAAA CAGCAGTCAG AGCTCTGCTT CTGAGGAAAC CGTGTTACGA 
TCTGATGCCC CTGATAGTAC AGGAGATCAG CCTGGACTCC ATCAAGAAAA TTCCTCAAAT 
GGAAAGTCTG AGTGGTCGGA TGCCTCCCAG AAGTCCCCTG TGCATGTCGG AGAGACGAGG 
AAGGAGGATG ACCCCAATGA AGACTGGTGT GCTGTTTGTC AAAATGGTGG GGAACTCCTA 
TGCTGTGAGA AATGTCCTAA AGTATTCCAT CTTACTTGTC ATGTGCCCAC CTTGACAAAT 
TTTCCAAGTG GAGAATGGAT CTGTACTTTC TGCCGAGACT TATCTAAGCC AGAGGTTGAC 
TATGATTGTG ATGTTCCCAG TCACCACTCA GAGAAACGGA AAAGTGAAGG CCTTACTAAG 
TTAACGCCAA TAGACAAAAG GAAATGTGAA CGCCTACTTC TGTTTCTTTA CTGCCATGAA 
ATGAGCCTGG CTTTCCAAGA CCCTGTTCCT CTAACTGTGC CTGATTATTA TAAAATAATT 
AAAAACCCAA TGGACTTGTC AACCATCAAG AAAAGACTTC AGGAGGATTA TTGCATGTAT 
ACAAAGCCTG AAGACTTTGT AGCTGATTTT AGATTGATCT TTCAAAACTG TGCTGAATTC 
AATGAGCCTG ATTCTGAAGT AGCCAATGCT GGTATAAAAC TTGAAAGCTA TTTTGAAGAA 
CTTCTAAAGA ATCTTTATCC AGAAAAAAGG TTTCCTAAGG TAGAATTCAG GCATGAAGCA 
GAAGACTGTA AGTTCAGTGA CGACTCAGAC GATGACTTTG TACAGCCCCG GAAGAAGCGT 
CTCAAGAGCA CCGAGGATCG CCAGCTGCTT AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trim24 ( NM_001272064.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001258993.1 Mus musculus tripartite motif-containing 24 (Trim24), transcript variant 2, mRNA.

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CloneID	OMu17139
Clone ID Related Accession (Same CDS sequence)	NM_001272064.1
Accession Version	NM_001272064.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	transcription intermediary factor 1-alpha isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from S78219.1 and AC113501.7. Transcript Variant: This variant (2) uses an alternate in-frame donor splice site in the coding region compared to variant 1. It encodes isoform 2 which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGTGG CTGTGGAGAA GGCGGCGGCG GCAGCGGCTC CGGCCGGAGG CCCCGCAGCG 
GCGGCGCCGA GCGGGGAGAA TGAGGCCGAG AGCCGGCAGG GCCCGGACTC GGAGAGCGGC 
GGCGAGGCGT CCCGGCTCAA CCTGTTGGAC ACTTGCGCCG TGTGCCACCA GAACATCCAG 
AGCCGGGTGC CCAAGCTGCT GCCCTGCCTG CACTCGTTCT GCCAGCGCTG TTTGCCCGCG 
CCGCAGCGCT ATCTCATGCT GACGGCGCCC GCGCTGGGCT CGGCAGAGAC CCCTCCACCC 
GCTCCCGCCC CCGCCCCCGC CCCGGGCTCC CCGGCCGGTG GTCCTTCGCC ATTCGCCACC 
CAAGTTGGAG TCATTCGATG CCCAGTTTGC AGTCAAGAGT GTGCTGAGAG ACACATCATA 
GACAACTTTT TTGTGAAGGA CACCACTGAA GTTCCTAGTA GTACAGTAGA AAAGTCTAAT 
CAGGTATGTA CAAGCTGTGA AGACAATGCA GAAGCTAATG GGTTTTGTGT AGAGTGTGTT 
GAATGGCTCT GCAAGACATG TATTAGAGCT CACCAGAGGG TGAAGTTCAC AAAAGACCAC 
ACAGTCAGGC AGAAAGAAGA AGTATCTCCA GAGGCAGTTG GGGTGACCAG TCAGCGACCA 
GTGTTTTGTC CCTTCCATAA AAAGGAGCAG TTGAAACTTT ACTGTGAAAC ATGTGATAAA 
CTGACCTGTC GAGACTGCCA GCTGCTAGAA CACAAAGAAC ACAGGTATCA ATTTATAGAA 
GAAGCTTTTC AGAATCAAAA AGTGATCATA GATACTCTAA TCACCAAACT GATGGAAAAA 
ACAAAATATA TAAAGTATAC AGGAAATCAG ATCCAAAATA GGATAATTGA AATAAATCAA 
AACCAAAAGC AGGTGGAACA GGATATTAAA GTTGCCATCT TCACATTGAT GGTGGAGATA 
AACAAAAAAG GGAAAGCTCT GCTGCACCAG CTTGAGAGTC TTGCAAAGGA CCATCGAATG 
AAACTCATGC AACAACAGCA GGAAGTGGCT GGGCTTTCTA AGCAGTTAGA GCACGTCATG 
CATTTTTCTA AATGGGCTGT TTCCAGTGGC AGCAGCACAG CCTTGCTGTA CAGCAAGCGG 
CTGATTACAT ACAGGTTACG GCACCTTCTT CGTGCAAGGT GTGATGCTTC TCCTGTGACC 
AACACCACCA TCCAGTTTCA CTGTGATCCT AGTTTCTGGG CTCAAAATAT TATCAACTTG 
GGTTCTTTAG TAATCGAGGA TAAAGAGAGC CAGCCACAAA TGCCTAAGCA GAATCCTGTC 
GTGGAGCAGA GTTCACAGCC ACCAGGTGGT TTACCTTCCA ACCAGTTATC CAAGTTCCCA 
ACACAGATCA GCCTAGCTCA GTTACGACTC CAGCATATTC AGCAACAGCA TCCGCCACCA 
CGCTTAATAA ACTTTCAGAA TCACAGCCCT AAGCCCAATG GACCAGTTCT TCCTCCTTAT 
CCTCAGCAGC TGAGATATTC ACCAAGCCAG AATGTACCTC GGCAGACAAC AATAAAGCCC 
AACCCCTTGC AAATGGCTTT TTTGGCTCAA CAGGCCATAA AACAGTGGCA GATCAGCAGT 
GTACAGGCTC CGCCCACAAC TGCCAGCAGC TCCTCCTCCA CGCCGTCCAG CCCCACAATC 
ACAAGTGCAG CTGGGTACGA TGGAAAAGCT TTTAGTTCAC CCATGATTGA TCTGAGTGCA 
CCGGTGGGAG GGTCTTACAA TCTTCCTTCT CTTCCAGATA TTGATTGTTC AAGTACTATA 
ATGTTGGACA ACATTGCAAG GAAAGACACA GGTGTAGATC ACGCCCAGCC GAGGCCTCCG 
TCAAACAGAA CGGTGCAGTC ACCAAATTCA TCAGTGCCAT CTCCAGGCCT TGCAGGGCCT 
GTTACTATGA CTAGCGTCCA TCCCCCAATA CGTTCACCTA GTGCCTCCAG TGTTGGAAGT 
CGAGGAAGCT CTGGCTCTTC CAGCAAACCA GCAGGAGCTG ATTCTACTCA CAAGGTCCCA 
GTAGTCATGC TGGAGCCAAT TCGAATAAAA CAGGAAAACA GTGGACCACC TGAAAATTAT 
GATTTTCCTG TTGTTATAGT AAAACAAGAA TCAGATGAAG AATCTAGACC TCAAAATACT 
AACTATCCAA GAAGCATACT TACCTCCCTC CTCTTAAACA GCAGTCAGAG CTCTGCTTCT 
GAGGAAACCG TGTTACGATC TGATGCCCCT GATAGTACAG GAGATCAGCC TGGACTCCAT 
CAAGAAAATT CCTCAAATGG AAAGTCTGAG TGGTCGGATG CCTCCCAGAA GTCCCCTGTG 
CATGTCGGAG AGACGAGGAA GGAGGATGAC CCCAATGAAG ACTGGTGTGC TGTTTGTCAA 
AATGGTGGGG AACTCCTATG CTGTGAGAAA TGTCCTAAAG TATTCCATCT TACTTGTCAT 
GTGCCCACCT TGACAAATTT TCCAAGTGGA GAATGGATCT GTACTTTCTG CCGAGACTTA 
TCTAAGCCAG AGGTTGACTA TGATTGTGAT GTTCCCAGTC ACCACTCAGA GAAACGGAAA 
AGTGAAGGCC TTACTAAGTT AACGCCAATA GACAAAAGGA AATGTGAACG CCTACTTCTG 
TTTCTTTACT GCCATGAAAT GAGCCTGGCT TTCCAAGACC CTGTTCCTCT AACTGTGCCT 
GATTATTATA AAATAATTAA AAACCCAATG GACTTGTCAA CCATCAAGAA AAGACTTCAG 
GAGGATTATT GCATGTATAC AAAGCCTGAA GACTTTGTAG CTGATTTTAG ATTGATCTTT 
CAAAACTGTG CTGAATTCAA TGAGCCTGAT TCTGAAGTAG CCAATGCTGG TATAAAACTT 
GAAAGCTATT TTGAAGAACT TCTAAAGAAT CTTTATCCAG AAAAAAGGTT TCCTAAGGTA 
GAATTCAGGC ATGAAGCAGA AGACTGTAAG TTCAGTGACG ACTCAGACGA TGACTTTGTA 
CAGCCCCGGA AGAAGCGTCT CAAGAGCACC GAGGATCGCC AGCTGCTTAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258993.1
CDS	301..3354
Translation	MEVAVEKAAAAAAPAGGPAAAAPSGENEAESRQGPDSESGGEASRLNLLDTCAVCHQNIQSRVPKLLPCLHSFCQRCLPAPQRYLMLTAPALGSAETPPPAPAPAPAPGSPAGGPSPFATQVGVIRCPVCSQECAERHIIDNFFVKDTTEVPSSTVEKSNQVCTSCEDNAEANGFCVECVEWLCKTCIRAHQRVKFTKDHTVRQKEEVSPEAVGVTSQRPVFCPFHKKEQLKLYCETCDKLTCRDCQLLEHKEHRYQFIEEAFQNQKVIIDTLITKLMEKTKYIKYTGNQIQNRIIEINQNQKQVEQDIKVAIFTLMVEINKKGKALLHQLESLAKDHRMKLMQQQQEVAGLSKQLEHVMHFSKWAVSSGSSTALLYSKRLITYRLRHLLRARCDASPVTNTTIQFHCDPSFWAQNIINLGSLVIEDKESQPQMPKQNPVVEQSSQPPGGLPSNQLSKFPTQISLAQLRLQHIQQQHPPPRLINFQNHSPKPNGPVLPPYPQQLRYSPSQNVPRQTTIKPNPLQMAFLAQQAIKQWQISSVQAPPTTASSSSSTPSSPTITSAAGYDGKAFSSPMIDLSAPVGGSYNLPSLPDIDCSSTIMLDNIARKDTGVDHAQPRPPSNRTVQSPNSSVPSPGLAGPVTMTSVHPPIRSPSASSVGSRGSSGSSSKPAGADSTHKVPVVMLEPIRIKQENSGPPENYDFPVVIVKQESDEESRPQNTNYPRSILTSLLLNSSQSSASEETVLRSDAPDSTGDQPGLHQENSSNGKSEWSDASQKSPVHVGETRKEDDPNEDWCAVCQNGGELLCCEKCPKVFHLTCHVPTLTNFPSGEWICTFCRDLSKPEVDYDCDVPSHHSEKRKSEGLTKLTPIDKRKCERLLLFLYCHEMSLAFQDPVPLTVPDYYKIIKNPMDLSTIKKRLQEDYCMYTKPEDFVADFRLIFQNCAEFNEPDSEVANAGIKLESYFEELLKNLYPEKRFPKVEFRHEAEDCKFSDDSDDDFVQPRKKRLKSTEDRQLLK

Target ORF information:

RefSeq Version	NM_001272064.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tripartite motif-containing 24 (Trim24), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272064.1

ORF Insert Sequence:

ATGGAGGTGG CTGTGGAGAA GGCGGCGGCG GCAGCGGCTC CGGCCGGAGG CCCCGCAGCG 
GCGGCGCCGA GCGGGGAGAA TGAGGCCGAG AGCCGGCAGG GCCCGGACTC GGAGAGCGGC 
GGCGAGGCGT CCCGGCTCAA CCTGTTGGAC ACTTGCGCCG TGTGCCACCA GAACATCCAG 
AGCCGGGTGC CCAAGCTGCT GCCCTGCCTG CACTCGTTCT GCCAGCGCTG TTTGCCCGCG 
CCGCAGCGCT ATCTCATGCT GACGGCGCCC GCGCTGGGCT CGGCAGAGAC CCCTCCACCC 
GCTCCCGCCC CCGCCCCCGC CCCGGGCTCC CCGGCCGGTG GTCCTTCGCC ATTCGCCACC 
CAAGTTGGAG TCATTCGATG CCCAGTTTGC AGTCAAGAGT GTGCTGAGAG ACACATCATA 
GACAACTTTT TTGTGAAGGA CACCACTGAA GTTCCTAGTA GTACAGTAGA AAAGTCTAAT 
CAGGTATGTA CAAGCTGTGA AGACAATGCA GAAGCTAATG GGTTTTGTGT AGAGTGTGTT 
GAATGGCTCT GCAAGACATG TATTAGAGCT CACCAGAGGG TGAAGTTCAC AAAAGACCAC 
ACAGTCAGGC AGAAAGAAGA AGTATCTCCA GAGGCAGTTG GGGTGACCAG TCAGCGACCA 
GTGTTTTGTC CCTTCCATAA AAAGGAGCAG TTGAAACTTT ACTGTGAAAC ATGTGATAAA 
CTGACCTGTC GAGACTGCCA GCTGCTAGAA CACAAAGAAC ACAGGTATCA ATTTATAGAA 
GAAGCTTTTC AGAATCAAAA AGTGATCATA GATACTCTAA TCACCAAACT GATGGAAAAA 
ACAAAATATA TAAAGTATAC AGGAAATCAG ATCCAAAATA GGATAATTGA AATAAATCAA 
AACCAAAAGC AGGTGGAACA GGATATTAAA GTTGCCATCT TCACATTGAT GGTGGAGATA 
AACAAAAAAG GGAAAGCTCT GCTGCACCAG CTTGAGAGTC TTGCAAAGGA CCATCGAATG 
AAACTCATGC AACAACAGCA GGAAGTGGCT GGGCTTTCTA AGCAGTTAGA GCACGTCATG 
CATTTTTCTA AATGGGCTGT TTCCAGTGGC AGCAGCACAG CCTTGCTGTA CAGCAAGCGG 
CTGATTACAT ACAGGTTACG GCACCTTCTT CGTGCAAGGT GTGATGCTTC TCCTGTGACC 
AACACCACCA TCCAGTTTCA CTGTGATCCT AGTTTCTGGG CTCAAAATAT TATCAACTTG 
GGTTCTTTAG TAATCGAGGA TAAAGAGAGC CAGCCACAAA TGCCTAAGCA GAATCCTGTC 
GTGGAGCAGA GTTCACAGCC ACCAGGTGGT TTACCTTCCA ACCAGTTATC CAAGTTCCCA 
ACACAGATCA GCCTAGCTCA GTTACGACTC CAGCATATTC AGCAACAGCA TCCGCCACCA 
CGCTTAATAA ACTTTCAGAA TCACAGCCCT AAGCCCAATG GACCAGTTCT TCCTCCTTAT 
CCTCAGCAGC TGAGATATTC ACCAAGCCAG AATGTACCTC GGCAGACAAC AATAAAGCCC 
AACCCCTTGC AAATGGCTTT TTTGGCTCAA CAGGCCATAA AACAGTGGCA GATCAGCAGT 
GTACAGGCTC CGCCCACAAC TGCCAGCAGC TCCTCCTCCA CGCCGTCCAG CCCCACAATC 
ACAAGTGCAG CTGGGTACGA TGGAAAAGCT TTTAGTTCAC CCATGATTGA TCTGAGTGCA 
CCGGTGGGAG GGTCTTACAA TCTTCCTTCT CTTCCAGATA TTGATTGTTC AAGTACTATA 
ATGTTGGACA ACATTGCAAG GAAAGACACA GGTGTAGATC ACGCCCAGCC GAGGCCTCCG 
TCAAACAGAA CGGTGCAGTC ACCAAATTCA TCAGTGCCAT CTCCAGGCCT TGCAGGGCCT 
GTTACTATGA CTAGCGTCCA TCCCCCAATA CGTTCACCTA GTGCCTCCAG TGTTGGAAGT 
CGAGGAAGCT CTGGCTCTTC CAGCAAACCA GCAGGAGCTG ATTCTACTCA CAAGGTCCCA 
GTAGTCATGC TGGAGCCAAT TCGAATAAAA CAGGAAAACA GTGGACCACC TGAAAATTAT 
GATTTTCCTG TTGTTATAGT AAAACAAGAA TCAGATGAAG AATCTAGACC TCAAAATACT 
AACTATCCAA GAAGCATACT TACCTCCCTC CTCTTAAACA GCAGTCAGAG CTCTGCTTCT 
GAGGAAACCG TGTTACGATC TGATGCCCCT GATAGTACAG GAGATCAGCC TGGACTCCAT 
CAAGAAAATT CCTCAAATGG AAAGTCTGAG TGGTCGGATG CCTCCCAGAA GTCCCCTGTG 
CATGTCGGAG AGACGAGGAA GGAGGATGAC CCCAATGAAG ACTGGTGTGC TGTTTGTCAA 
AATGGTGGGG AACTCCTATG CTGTGAGAAA TGTCCTAAAG TATTCCATCT TACTTGTCAT 
GTGCCCACCT TGACAAATTT TCCAAGTGGA GAATGGATCT GTACTTTCTG CCGAGACTTA 
TCTAAGCCAG AGGTTGACTA TGATTGTGAT GTTCCCAGTC ACCACTCAGA GAAACGGAAA 
AGTGAAGGCC TTACTAAGTT AACGCCAATA GACAAAAGGA AATGTGAACG CCTACTTCTG 
TTTCTTTACT GCCATGAAAT GAGCCTGGCT TTCCAAGACC CTGTTCCTCT AACTGTGCCT 
GATTATTATA AAATAATTAA AAACCCAATG GACTTGTCAA CCATCAAGAA AAGACTTCAG 
GAGGATTATT GCATGTATAC AAAGCCTGAA GACTTTGTAG CTGATTTTAG ATTGATCTTT 
CAAAACTGTG CTGAATTCAA TGAGCCTGAT TCTGAAGTAG CCAATGCTGG TATAAAACTT 
GAAAGCTATT TTGAAGAACT TCTAAAGAAT CTTTATCCAG AAAAAAGGTT TCCTAAGGTA 
GAATTCAGGC ATGAAGCAGA AGACTGTAAG TTCAGTGACG ACTCAGACGA TGACTTTGTA 
CAGCCCCGGA AGAAGCGTCT CAAGAGCACC GAGGATCGCC AGCTGCTTAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expanding the Circuitry of Pluripotency by Selective Isolation of Chromatin-Associated Proteins.
Molecular cell64(3)624-635(2016 Nov)
Rafiee MR,Girardot C,Sigismondo G,Krijgsveld J

Novel gene function revealed by mouse mutagenesis screens for models of age-related disease.
Nature communications712444(2016 Aug)
Potter PK,Bowl MR,Jeyarajan P,Wisby L,Blease A,Goldsworthy ME,Simon MM,Greenaway S,Michel V,Barnard A,Aguilar C,Agnew T,Banks G,Blake A,Chessum L,Dorning J,Falcone S,Goosey L,Harris S,Haynes A,Heise I,Hillier R,Hough T,Hoslin A,Hutchison M,King R,Kumar S,Lad HV,Law G,MacLaren RE,Morse S,Nicol T,Parker A,Pickford K,Sethi S,Starbuck B,Stelma F,Cheeseman M,Cross SH,Foster RG,Jackson IJ,Peirson SN,Thakker RV,Vincent T,Scudamore C,Wells S,El-Amraoui A,Petit C,Acevedo-Arozena A,Nolan PM,Cox R,Mallon AM,Brown SD

T-cell-intrinsic Tif1?/Trim24 regulates IL-1R expression on TH2 cells and TH2 cell-mediated airway allergy.
Proceedings of the National Academy of Sciences of the United States of America113(5)E568-76(2016 Feb)
Perez-Lloret J,Okoye IS,Guidi R,Kannan Y,Coomes SM,Czieso S,Mengus G,Davidson I,Wilson MS

Trim24-repressed VL30 retrotransposons regulate gene expression by producing noncoding RNA.
Nature structural & molecular biology20(3)339-46(2013 Mar)
Herquel B,Ouararhni K,Martianov I,Le Gras S,Ye T,Keime C,Lerouge T,Jost B,Cammas F,Losson R,Davidson I

Interaction with members of the heterochromatin protein 1 (HP1) family and histone deacetylation are differentially involved in transcriptional silencing by members of the TIF1 family.
The EMBO journal18(22)6385-95(1999 Nov)
Nielsen AL,Ortiz JA,You J,Oulad-Abdelghani M,Khechumian R,Gansmuller A,Chambon P,Losson R

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Trim24 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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