Nek9 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nek9
Entrez Gene ID	217718
Full Name	NIMA (never in mitosis gene a)-related expressed kinase 9
Synonyms	C130021H08Rik
General protein information	Preferred Names NIMA (never in mitosis gene a)-related expressed kinase 9 Names serine/threonine-protein kinase Nek9 nercc1 kinase never in mitosis A-related kinase 9 nimA-related protein kinase 9
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 D1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006515708.4	XP_006515771.1	serine/threonine-protein kinase Nek9 isoform X1
NM_145138.2	NP_660120.2	serine/threonine-protein kinase Nek9
XM_006515708.3	XP_006515771.1	serine/threonine-protein kinase Nek9 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Nek9	NP_001100217.1
Gallus gallus (chicken)	NEK9	XP_001235085.3
Homo sapiens (human)	NEK9	NP_149107.4
	NEK9	XP_001097546.1
Canis lupus familiaris (dog)	NEK9	XP_547912.3
Bos taurus (cattle)	NEK9	NP_001192989.1
Mus musculus (house mouse)	Nek9	NP_660120.2
Pan troglodytes (chimpanzee)	NEK9	XP_003314506.1
Danio rerio (zebrafish)	LOC100334361	XP_002665318.3
Xenopus tropicalis (tropical clawed frog)	nek9	NP_001135671.1

Gene Ontology
Bibliography

Related articles in PubMed

A gemcitabine sensitivity screen identifies a role for NEK9 in the replication stress response.
Smith SC, Petrova AV, Madden MZ, Wang H, Pan Y, Warren MD, Hardy CW, Liang D, Liu EA, Robinson MH, Rudra S, Wang J, Ehdaivand S, Torres MA, Wang Y, Yu DS
Nucleic acids research42(18)11517-27(2014 Oct)

Nek9 regulates spindle organization and cell cycle progression during mouse oocyte meiosis and its location in early embryo mitosis.
Yang SW, Gao C, Chen L, Song YL, Zhu JL, Qi ST, Jiang ZZ, Wang ZW, Lin F, Huang H, Xing FQ, Sun QY
Cell cycle (Georgetown, Tex.)11(23)4366-77(2012 Dec)

Adenovirus E1A proteins direct subcellular redistribution of Nek9, a NimA-related kinase.
Pelka P, Scim? A, Mandalfino C, Joch M, Abdulla P, Whyte P
Journal of cellular physiology212(1)13-25(2007 Jul)

Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
Okazaki N, F-Kikuno R, Ohara R, Inamoto S, Koseki H, Hiraoka S, Saga Y, Seino S, Nishimura M, Kaisho T, Hoshino K, Kitamura H, Nagase T, Ohara O, Koga H
DNA research : an international journal for rapid publication of reports on genes and genomes11(3)205-18(2004 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NEK9 is a critical component of the replication stress response that promotes CHK1 activity, maintaining genome integrity following challenges to DNA replication.
Title: A gemcitabine sensitivity screen identifies a role for NEK9 in the replication stress response.

Knockdown of Nek9 also impaired the spindle-pole localization of gamma-tubulin and resulted in retention of the spindle assembly checkpoint protein Bub3 at the kinetochores.
Title: Nek9 regulates spindle organization and cell cycle progression during mouse oocyte meiosis and its location in early embryo mitosis.

The following Nek9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nek9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu11335	NM_145138.2 Latest version!	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 9 (Nek9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$580.30 $829.00
OMu47735	XM_006515708.4 Latest version!	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 9 (Nek9), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu47735	XM_006515708.3	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 9 (Nek9), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nek9 ( XM_006515708.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515771.1 Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 9 (Nek9), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu47735
Clone ID Related Accession (Same CDS sequence)	XM_006515708.4 , XM_006515708.3
Accession Version	XM_006515708.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase Nek9 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515708.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGTGG GCACTCCATA TTACATGTCT CCAGAGCTCT GCCAAGGAGT AAAATACAAT 
TTCAAGTCTG ATATATGGGC AGTTGGCTGT GTCATTTTTG AACTGCTTAC TCTGAAAAGA 
ACATTTGATG CTACAAACCC ACTCAACCTA TGTGTGAAGA TCGTGCAGGG AATCCGGGCC 
ATGGAAGTTG ACTCGAGTCA GTATTCTTTG GAACTGATCC AGTTGGTACA CGCATGCCTC 
GACCAGGATC CTGAGCAGAG GCCCGCTGCA GATGCACTCC TGGATCTCCC CCTTCTCAGG 
ACCCGCAGGA GAGAGATGGA GGAAAAAGTC ACTCTGCTCA ATGCACCTAC AAAGAGACCA 
AGGTCAAGCA CTGTGACGGA AGCACCCATT GCTGTGGTAA CATCACGAAC CAGTGAAGTC 
TATGTTTGGG GTGGTGGAAA ATCCACACCT CAGAAATTGG ACGTCATCAA GAGTGGCTGT 
AGTGCTCGAC AGGTGTGTGC GGGGAACACC CACTTTGCTG TGGTCACCGT GGAGAAGGAA 
CTATATACCT GGGTGAACAT GCAAGGGGGC ACTAAACTTC ACGGTCAGCT AGGACATGGA 
GACAAAGCTT CCTACCGACA GCCAAAGCAT GTAGAAAAGT TACAAGGGAA GGCTATCCAC 
CAGGTGTCGT GTGGGGATGA TTTCACTGTC TGTGTCACAG ATGAGGGTCA GCTCTACGCC 
TTTGGATCAG ATTATTATGG CTGCATGGGA GTGGACAAGG TTTCTGGCCC TGAAGTGCTA 
GAACCCATGC AGCTGAACTT CTTCCTCAGC AATCCTGTGG AGCAGGTCTC CTGTGGGGAT 
AATCATGTGG TAGTTCTGAC ACGAAACAAA GAAGTCTACT CTTGGGGCTG TGGTGAATAT 
GGACGGCTGG GTTTGGATTC AGAAGAGGAT TACTATACAC CACAGAGGGT GGATGTTCCA 
AAGGCCTTAA TCATTGTTGC AGTTCAATGT GGCTGTGATG GGACCTTTCT GCTCACTCAG 
TCAGGCAAAG TGCTGGCCTG TGGACTCAAT GAGTTCAACA AGCTGGGGCT GAATCAGTGC 
ATGTCTGGAA TCATCAACCA TGAAGCATAC CATGAAGTTC CCTACACAAC CTCCTTTACC 
TTGGCCAAAC AGTTGTCCTT TTACAAAATC CGTACCATTG CCCCAGGCAA GACTCACACA 
GCTGCTATTG ATGAACGAGG CCGGTTGCTG ACTTTTGGCT GTAATAAGTG TGGGCAGCTG 
GGTGTTGGGA ATTATAAGAA ACGCCTGGGA ATCAACCTGT TGGGGGGACC CCTTGGCGGG 
AAGCAAGTGA TTAGGGTCTC GTGTGGCGAT GAGTTCACCA TTGCTGCCAC CGACGATAAT 
CACATCTTTG CCTGGGGTAA TGGTGGTAAT GGCCGCCTGG CAATGACTCC CACAGAGAGA 
CCACATGGCT CTGATATCTG TACCTCATGG CCTCGGCCTA TTTTTGGATC CCTGCATCAT 
GTTCCAGACC TTTCTTGCCG TGGCTGGCAC ACCATTCTTA TTGTTGAGAA AGTGTTGAAT 
TCCAAGACCA TCCGTTCCAA TAGCAGTGGC CTGTCCATCG GAACTGTGGT TCAGAGCTCC 
AGCCCAGGAG GTGGCATTGG TGGTGGTGGT GGTGGTGGCG GCGGCGGCGG GGGTGAAGAG 
GAAGACAGTC AACAGGAATC TGAAACTCCA GATCCAAGCG GAGGCTTCCG AGGAACAATG 
GAAGCAGACC GTGGGATGGA AGGCTTAATC AGTCCCACGG AGGCCGTGGG AAACAGCTGT 
GGGGCTAGCA GCTCCTGTCC TGGCTGGCTT CGGAAGGAGC TGGAAAACGC AGAGTTCATT 
CCCATGCCTG ACAGCCCCGC TCCCTTAAGT GCGGCGTTTT CACAGTCTGA GAAGGACACG 
CTGCCCTATG AAGAGCTGCA GGGACTCAAG GTCGCATCTG AAGTTCCTCC GGAGCCCCAG 
CGTGCAGCAG GAGCCTGGCC CCCTCGGCTG GATCCTGCAG TGCCGTGTGT TGGGAAAGCC 
CTGACCTCTG CTGCTTGTGC GTGCAGTGCT CTGCAGGTGG AGGTTGACAG GTTGCAGGCT 
CTGGTGTTAA AGTGTCTGGA AGAACAGCAG AAGCTCCAGC AAGAAAACCT CCAGATGTTT 
ACCCAACTAC AAAAGCTCAA CAAGAAATTG GAAGGAGGCC AGCAGGTGGG GATGCACTCC 
AGAGGAACCC AGACAGCAAA GGAAGAAATG GAAATGGATC CAAAGCCTGA CTTAGACTCG 
GAGTCCTGGT GCCTCCTCGG AACAGACTCT TGTCGACCCA GCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515771.1
CDS	490..2817
Translation	MLVGTPYYMSPELCQGVKYNFKSDIWAVGCVIFELLTLKRTFDATNPLNLCVKIVQGIRAMEVDSSQYSLELIQLVHACLDQDPEQRPAADALLDLPLLRTRRREMEEKVTLLNAPTKRPRSSTVTEAPIAVVTSRTSEVYVWGGGKSTPQKLDVIKSGCSARQVCAGNTHFAVVTVEKELYTWVNMQGGTKLHGQLGHGDKASYRQPKHVEKLQGKAIHQVSCGDDFTVCVTDEGQLYAFGSDYYGCMGVDKVSGPEVLEPMQLNFFLSNPVEQVSCGDNHVVVLTRNKEVYSWGCGEYGRLGLDSEEDYYTPQRVDVPKALIIVAVQCGCDGTFLLTQSGKVLACGLNEFNKLGLNQCMSGIINHEAYHEVPYTTSFTLAKQLSFYKIRTIAPGKTHTAAIDERGRLLTFGCNKCGQLGVGNYKKRLGINLLGGPLGGKQVIRVSCGDEFTIAATDDNHIFAWGNGGNGRLAMTPTERPHGSDICTSWPRPIFGSLHHVPDLSCRGWHTILIVEKVLNSKTIRSNSSGLSIGTVVQSSSPGGGIGGGGGGGGGGGGEEEDSQQESETPDPSGGFRGTMEADRGMEGLISPTEAVGNSCGASSSCPGWLRKELENAEFIPMPDSPAPLSAAFSQSEKDTLPYEELQGLKVASEVPPEPQRAAGAWPPRLDPAVPCVGKALTSAACACSALQVEVDRLQALVLKCLEEQQKLQQENLQMFTQLQKLNKKLEGGQQVGMHSRGTQTAKEEMEMDPKPDLDSESWCLLGTDSCRPSL

Target ORF information:

RefSeq Version	XM_006515708.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 9 (Nek9), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515708.3

ORF Insert Sequence:

ATGCTTGTGG GCACTCCATA TTACATGTCT CCAGAGCTCT GCCAAGGAGT AAAATACAAT 
TTCAAGTCTG ATATATGGGC AGTTGGCTGT GTCATTTTTG AACTGCTTAC TCTGAAAAGA 
ACATTTGATG CTACAAACCC ACTCAACCTA TGTGTGAAGA TCGTGCAGGG AATCCGGGCC 
ATGGAAGTTG ACTCGAGTCA GTATTCTTTG GAACTGATCC AGTTGGTACA CGCATGCCTC 
GACCAGGATC CTGAGCAGAG GCCCGCTGCA GATGCACTCC TGGATCTCCC CCTTCTCAGG 
ACCCGCAGGA GAGAGATGGA GGAAAAAGTC ACTCTGCTCA ATGCACCTAC AAAGAGACCA 
AGGTCAAGCA CTGTGACGGA AGCACCCATT GCTGTGGTAA CATCACGAAC CAGTGAAGTC 
TATGTTTGGG GTGGTGGAAA ATCCACACCT CAGAAATTGG ACGTCATCAA GAGTGGCTGT 
AGTGCTCGAC AGGTGTGTGC GGGGAACACC CACTTTGCTG TGGTCACCGT GGAGAAGGAA 
CTATATACCT GGGTGAACAT GCAAGGGGGC ACTAAACTTC ACGGTCAGCT AGGACATGGA 
GACAAAGCTT CCTACCGACA GCCAAAGCAT GTAGAAAAGT TACAAGGGAA GGCTATCCAC 
CAGGTGTCGT GTGGGGATGA TTTCACTGTC TGTGTCACAG ATGAGGGTCA GCTCTACGCC 
TTTGGATCAG ATTATTATGG CTGCATGGGA GTGGACAAGG TTTCTGGCCC TGAAGTGCTA 
GAACCCATGC AGCTGAACTT CTTCCTCAGC AATCCTGTGG AGCAGGTCTC CTGTGGGGAT 
AATCATGTGG TAGTTCTGAC ACGAAACAAA GAAGTCTACT CTTGGGGCTG TGGTGAATAT 
GGACGGCTGG GTTTGGATTC AGAAGAGGAT TACTATACAC CACAGAGGGT GGATGTTCCA 
AAGGCCTTAA TCATTGTTGC AGTTCAATGT GGCTGTGATG GGACCTTTCT GCTCACTCAG 
TCAGGCAAAG TGCTGGCCTG TGGACTCAAT GAGTTCAACA AGCTGGGGCT GAATCAGTGC 
ATGTCTGGAA TCATCAACCA TGAAGCATAC CATGAAGTTC CCTACACAAC CTCCTTTACC 
TTGGCCAAAC AGTTGTCCTT TTACAAAATC CGTACCATTG CCCCAGGCAA GACTCACACA 
GCTGCTATTG ATGAACGAGG CCGGTTGCTG ACTTTTGGCT GTAATAAGTG TGGGCAGCTG 
GGTGTTGGGA ATTATAAGAA ACGCCTGGGA ATCAACCTGT TGGGGGGACC CCTTGGCGGG 
AAGCAAGTGA TTAGGGTCTC GTGTGGCGAT GAGTTCACCA TTGCTGCCAC CGACGATAAT 
CACATCTTTG CCTGGGGTAA TGGTGGTAAT GGCCGCCTGG CAATGACTCC CACAGAGAGA 
CCACATGGCT CTGATATCTG TACCTCATGG CCTCGGCCTA TTTTTGGATC CCTGCATCAT 
GTTCCAGACC TTTCTTGCCG TGGCTGGCAC ACCATTCTTA TTGTTGAGAA AGTGTTGAAT 
TCCAAGACCA TCCGTTCCAA TAGCAGTGGC CTGTCCATCG GAACTGTGGT TCAGAGCTCC 
AGCCCAGGAG GTGGCATTGG TGGTGGTGGT GGTGGTGGCG GCGGCGGCGG GGGTGAAGAG 
GAAGACAGTC AACAGGAATC TGAAACTCCA GATCCAAGCG GAGGCTTCCG AGGAACAATG 
GAAGCAGACC GTGGGATGGA AGGCTTAATC AGTCCCACGG AGGCCGTGGG AAACAGCTGT 
GGGGCTAGCA GCTCCTGTCC TGGCTGGCTT CGGAAGGAGC TGGAAAACGC AGAGTTCATT 
CCCATGCCTG ACAGCCCCGC TCCCTTAAGT GCGGCGTTTT CACAGTCTGA GAAGGACACG 
CTGCCCTATG AAGAGCTGCA GGGACTCAAG GTCGCATCTG AAGTTCCTCC GGAGCCCCAG 
CGTGCAGCAG GAGCCTGGCC CCCTCGGCTG GATCCTGCAG TGCCGTGTGT TGGGAAAGCC 
CTGACCTCTG CTGCTTGTGC GTGCAGTGCT CTGCAGGTGG AGGTTGACAG GTTGCAGGCT 
CTGGTGTTAA AGTGTCTGGA AGAACAGCAG AAGCTCCAGC AAGAAAACCT CCAGATGTTT 
ACCCAACTAC AAAAGCTCAA CAAGAAATTG GAAGGAGGCC AGCAGGTGGG GATGCACTCC 
AGAGGAACCC AGACAGCAAA GGAAGAAATG GAAATGGATC CAAAGCCTGA CTTAGACTCG 
GAGTCCTGGT GCCTCCTCGG AACAGACTCT TGTCGACCCA GCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nek9 ( NM_145138.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_660120.2 Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 9 (Nek9), mRNA.

Price & Availability↑

CloneID	OMu11335
Clone ID Related Accession (Same CDS sequence)	NM_145138.2
Accession Version	NM_145138.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2955bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase Nek9
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY343854.1, AK173323.1, BQ964444.1 and CD775651.1. On Oct 24, 2007 this sequence version replaced NM_145138.1. ##Evidence-Data-START## Transcript exon combination :: AJ489828.1, AK173323.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGTGC TGGGCGAGTA CGAGCGACAC TGCGATTCCA TCAACTCGGA CTTCGGAAGC 
GAGTCCGGGG GTGGCGGGGA CTCGGGCCCG GGCCCCAGCG CTGTCCCAGG GCCGCGAGCC 
GGCGGCGGGG CGGCGGAGCA GGAGGAGCTG CACTACATCC CCATCCGCGT TCTGGGCCGC 
GGTGCCTTTG GGGAGGCCAC GCTGTACCGC CGCACCGAGG ACGACTCACT GGTGGTGTGG 
AAGGAAGTTG ACTTGACCCG GCTATCTGAG AAGGAACGTC GAGATGCCCT GAATGAGATT 
GTCATCCTGG CACTGCTACA GCACGACAAC ATCATTGCCT ACTACAACCA CTTCATGGAC 
AACACCACCC TGCTGATTGA GCTGGAGTAC TGTAACGGAG GGAACCTCTA TGACAAAATC 
CTTCGTCAGA AGGACAAGTT GTTCGAAGAA GAGATGGTGG TGTGGTACCT TTTTCAGATT 
GTTTCAGCAG TGAGCTGTAT TCATAAAGCT GGAATCCTCC ATAGAGATAT AAAGACATTA 
AATATTTTTC TGACCAAGGC AAATCTGATA AAGCTTGGAG ACTATGGCCT AGCAAAGAAA 
CTTAATTCTG AGTACTCTAT GGCCGAGACA CTTGTGGGCA CTCCATATTA CATGTCTCCA 
GAGCTCTGCC AAGGAGTAAA ATACAATTTC AAGTCTGATA TATGGGCAGT TGGCTGTGTC 
ATTTTTGAAC TGCTTACTCT GAAAAGAACA TTTGATGCTA CAAACCCACT CAACCTATGT 
GTGAAGATCG TGCAGGGAAT CCGGGCCATG GAAGTTGACT CGAGTCAGTA TTCTTTGGAA 
CTGATCCAGT TGGTACACGC ATGCCTCGAC CAGGATCCTG AGCAGAGGCC CGCTGCAGAT 
GCACTCCTGG ATCTCCCCCT TCTCAGGACC CGCAGGAGAG AGATGGAGGA AAAAGTCACT 
CTGCTCAATG CACCTACAAA GAGACCAAGG TCAAGCACTG TGACGGAAGC ACCCATTGCT 
GTGGTAACAT CACGAACCAG TGAAGTCTAT GTTTGGGGTG GTGGAAAATC CACACCTCAG 
AAATTGGACG TCATCAAGAG TGGCTGTAGT GCTCGACAGG TGTGTGCGGG GAACACCCAC 
TTTGCTGTGG TCACCGTGGA GAAGGAACTA TATACCTGGG TGAACATGCA AGGGGGCACT 
AAACTTCACG GTCAGCTAGG ACATGGAGAC AAAGCTTCCT ACCGACAGCC AAAGCATGTA 
GAAAAGTTAC AAGGGAAGGC TATCCACCAG GTGTCGTGTG GGGATGATTT CACTGTCTGT 
GTCACAGATG AGGGTCAGCT CTACGCCTTT GGATCAGATT ATTATGGCTG CATGGGAGTG 
GACAAGGTTT CTGGCCCTGA AGTGCTAGAA CCCATGCAGC TGAACTTCTT CCTCAGCAAT 
CCTGTGGAGC AGGTCTCCTG TGGGGATAAT CATGTGGTAG TTCTGACACG AAACAAAGAA 
GTCTACTCTT GGGGCTGTGG TGAATATGGA CGGCTGGGTT TGGATTCAGA AGAGGATTAC 
TATACACCAC AGAGGGTGGA TGTTCCAAAG GCCTTAATCA TTGTTGCAGT TCAATGTGGC 
TGTGATGGGA CCTTTCTGCT CACTCAGTCA GGCAAAGTGC TGGCCTGTGG ACTCAATGAG 
TTCAACAAGC TGGGGCTGAA TCAGTGCATG TCTGGAATCA TCAACCATGA AGCATACCAT 
GAAGTTCCCT ACACAACCTC CTTTACCTTG GCCAAACAGT TGTCCTTTTA CAAAATCCGT 
ACCATTGCCC CAGGCAAGAC TCACACAGCT GCTATTGATG AACGAGGCCG GTTGCTGACT 
TTTGGCTGTA ATAAGTGTGG GCAGCTGGGT GTTGGGAATT ATAAGAAACG CCTGGGAATC 
AACCTGTTGG GGGGACCCCT TGGCGGGAAG CAAGTGATTA GGGTCTCGTG TGGCGATGAG 
TTCACCATTG CTGCCACCGA CGATAATCAC ATCTTTGCCT GGGGTAATGG TGGTAATGGC 
CGCCTGGCAA TGACTCCCAC AGAGAGACCA CATGGCTCTG ATATCTGTAC CTCATGGCCT 
CGGCCTATTT TTGGATCCCT GCATCATGTT CCAGACCTTT CTTGCCGTGG CTGGCACACC 
ATTCTTATTG TTGAGAAAGT GTTGAATTCC AAGACCATCC GTTCCAATAG CAGTGGCCTG 
TCCATCGGAA CTGTGGTTCA GAGCTCCAGC CCAGGAGGTG GCATTGGTGG TGGTGGTGGT 
GGTGGCGGCG GCGGCGGGGG TGAAGAGGAA GACAGTCAAC AGGAATCTGA AACTCCAGAT 
CCAAGCGGAG GCTTCCGAGG AACAATGGAA GCAGACCGTG GGATGGAAGG CTTAATCAGT 
CCCACGGAGG CCGTGGGAAA CAGCTGTGGG GCTAGCAGCT CCTGTCCTGG CTGGCTTCGG 
AAGGAGCTGG AAAACGCAGA GTTCATTCCC ATGCCTGACA GCCCCGCTCC CTTAAGTGCG 
GCGTTTTCAC AGTCTGAGAA GGACACGCTG CCCTATGAAG AGCTGCAGGG ACTCAAGGTC 
GCATCTGAAG TTCCTCCGGA GCCCCAGCGT GCAGCAGGAG CCTGGCCCCC TCGGCTGGAT 
CCTGCAGTGC CGTGTGTTGG GAAAGCCCTG ACCTCTGCTG CTTGTGCGTG CAGTGCTCTG 
CAGGTGGAGG TTGACAGGTT GCAGGCTCTG GTGTTAAAGT GTCTGGAAGA ACAGCAGAAG 
CTCCAGCAAG AAAACCTCCA GATGTTTACC CAACTACAAA AGCTCAACAA GAAATTGGAA 
GGAGGCCAGC AGGTGGGGAT GCACTCCAGA GGAACCCAGA CAGCAAAGGA AGAAATGGAA 
ATGGATCCAA AGCCTGACTT AGACTCGGAG TCCTGGTGCC TCCTCGGAAC AGACTCTTGT 
CGACCCAGCC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_660120.2
CDS	142..3096
Translation	MSVLGEYERHCDSINSDFGSESGGGGDSGPGPSAVPGPRAGGGAAEQEELHYIPIRVLGRGAFGEATLYRRTEDDSLVVWKEVDLTRLSEKERRDALNEIVILALLQHDNIIAYYNHFMDNTTLLIELEYCNGGNLYDKILRQKDKLFEEEMVVWYLFQIVSAVSCIHKAGILHRDIKTLNIFLTKANLIKLGDYGLAKKLNSEYSMAETLVGTPYYMSPELCQGVKYNFKSDIWAVGCVIFELLTLKRTFDATNPLNLCVKIVQGIRAMEVDSSQYSLELIQLVHACLDQDPEQRPAADALLDLPLLRTRRREMEEKVTLLNAPTKRPRSSTVTEAPIAVVTSRTSEVYVWGGGKSTPQKLDVIKSGCSARQVCAGNTHFAVVTVEKELYTWVNMQGGTKLHGQLGHGDKASYRQPKHVEKLQGKAIHQVSCGDDFTVCVTDEGQLYAFGSDYYGCMGVDKVSGPEVLEPMQLNFFLSNPVEQVSCGDNHVVVLTRNKEVYSWGCGEYGRLGLDSEEDYYTPQRVDVPKALIIVAVQCGCDGTFLLTQSGKVLACGLNEFNKLGLNQCMSGIINHEAYHEVPYTTSFTLAKQLSFYKIRTIAPGKTHTAAIDERGRLLTFGCNKCGQLGVGNYKKRLGINLLGGPLGGKQVIRVSCGDEFTIAATDDNHIFAWGNGGNGRLAMTPTERPHGSDICTSWPRPIFGSLHHVPDLSCRGWHTILIVEKVLNSKTIRSNSSGLSIGTVVQSSSPGGGIGGGGGGGGGGGGEEEDSQQESETPDPSGGFRGTMEADRGMEGLISPTEAVGNSCGASSSCPGWLRKELENAEFIPMPDSPAPLSAAFSQSEKDTLPYEELQGLKVASEVPPEPQRAAGAWPPRLDPAVPCVGKALTSAACACSALQVEVDRLQALVLKCLEEQQKLQQENLQMFTQLQKLNKKLEGGQQVGMHSRGTQTAKEEMEMDPKPDLDSESWCLLGTDSCRPSL

Target ORF information:

RefSeq Version	NM_145138.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 9 (Nek9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145138.2

ORF Insert Sequence:

ATGTCGGTGC TGGGCGAGTA CGAGCGACAC TGCGATTCCA TCAACTCGGA CTTCGGAAGC 
GAGTCCGGGG GTGGCGGGGA CTCGGGCCCG GGCCCCAGCG CTGTCCCAGG GCCGCGAGCC 
GGCGGCGGGG CGGCGGAGCA GGAGGAGCTG CACTACATCC CCATCCGCGT TCTGGGCCGC 
GGTGCCTTTG GGGAGGCCAC GCTGTACCGC CGCACCGAGG ACGACTCACT GGTGGTGTGG 
AAGGAAGTTG ACTTGACCCG GCTATCTGAG AAGGAACGTC GAGATGCCCT GAATGAGATT 
GTCATCCTGG CACTGCTACA GCACGACAAC ATCATTGCCT ACTACAACCA CTTCATGGAC 
AACACCACCC TGCTGATTGA GCTGGAGTAC TGTAACGGAG GGAACCTCTA TGACAAAATC 
CTTCGTCAGA AGGACAAGTT GTTCGAAGAA GAGATGGTGG TGTGGTACCT TTTTCAGATT 
GTTTCAGCAG TGAGCTGTAT TCATAAAGCT GGAATCCTCC ATAGAGATAT AAAGACATTA 
AATATTTTTC TGACCAAGGC AAATCTGATA AAGCTTGGAG ACTATGGCCT AGCAAAGAAA 
CTTAATTCTG AGTACTCTAT GGCCGAGACA CTTGTGGGCA CTCCATATTA CATGTCTCCA 
GAGCTCTGCC AAGGAGTAAA ATACAATTTC AAGTCTGATA TATGGGCAGT TGGCTGTGTC 
ATTTTTGAAC TGCTTACTCT GAAAAGAACA TTTGATGCTA CAAACCCACT CAACCTATGT 
GTGAAGATCG TGCAGGGAAT CCGGGCCATG GAAGTTGACT CGAGTCAGTA TTCTTTGGAA 
CTGATCCAGT TGGTACACGC ATGCCTCGAC CAGGATCCTG AGCAGAGGCC CGCTGCAGAT 
GCACTCCTGG ATCTCCCCCT TCTCAGGACC CGCAGGAGAG AGATGGAGGA AAAAGTCACT 
CTGCTCAATG CACCTACAAA GAGACCAAGG TCAAGCACTG TGACGGAAGC ACCCATTGCT 
GTGGTAACAT CACGAACCAG TGAAGTCTAT GTTTGGGGTG GTGGAAAATC CACACCTCAG 
AAATTGGACG TCATCAAGAG TGGCTGTAGT GCTCGACAGG TGTGTGCGGG GAACACCCAC 
TTTGCTGTGG TCACCGTGGA GAAGGAACTA TATACCTGGG TGAACATGCA AGGGGGCACT 
AAACTTCACG GTCAGCTAGG ACATGGAGAC AAAGCTTCCT ACCGACAGCC AAAGCATGTA 
GAAAAGTTAC AAGGGAAGGC TATCCACCAG GTGTCGTGTG GGGATGATTT CACTGTCTGT 
GTCACAGATG AGGGTCAGCT CTACGCCTTT GGATCAGATT ATTATGGCTG CATGGGAGTG 
GACAAGGTTT CTGGCCCTGA AGTGCTAGAA CCCATGCAGC TGAACTTCTT CCTCAGCAAT 
CCTGTGGAGC AGGTCTCCTG TGGGGATAAT CATGTGGTAG TTCTGACACG AAACAAAGAA 
GTCTACTCTT GGGGCTGTGG TGAATATGGA CGGCTGGGTT TGGATTCAGA AGAGGATTAC 
TATACACCAC AGAGGGTGGA TGTTCCAAAG GCCTTAATCA TTGTTGCAGT TCAATGTGGC 
TGTGATGGGA CCTTTCTGCT CACTCAGTCA GGCAAAGTGC TGGCCTGTGG ACTCAATGAG 
TTCAACAAGC TGGGGCTGAA TCAGTGCATG TCTGGAATCA TCAACCATGA AGCATACCAT 
GAAGTTCCCT ACACAACCTC CTTTACCTTG GCCAAACAGT TGTCCTTTTA CAAAATCCGT 
ACCATTGCCC CAGGCAAGAC TCACACAGCT GCTATTGATG AACGAGGCCG GTTGCTGACT 
TTTGGCTGTA ATAAGTGTGG GCAGCTGGGT GTTGGGAATT ATAAGAAACG CCTGGGAATC 
AACCTGTTGG GGGGACCCCT TGGCGGGAAG CAAGTGATTA GGGTCTCGTG TGGCGATGAG 
TTCACCATTG CTGCCACCGA CGATAATCAC ATCTTTGCCT GGGGTAATGG TGGTAATGGC 
CGCCTGGCAA TGACTCCCAC AGAGAGACCA CATGGCTCTG ATATCTGTAC CTCATGGCCT 
CGGCCTATTT TTGGATCCCT GCATCATGTT CCAGACCTTT CTTGCCGTGG CTGGCACACC 
ATTCTTATTG TTGAGAAAGT GTTGAATTCC AAGACCATCC GTTCCAATAG CAGTGGCCTG 
TCCATCGGAA CTGTGGTTCA GAGCTCCAGC CCAGGAGGTG GCATTGGTGG TGGTGGTGGT 
GGTGGCGGCG GCGGCGGGGG TGAAGAGGAA GACAGTCAAC AGGAATCTGA AACTCCAGAT 
CCAAGCGGAG GCTTCCGAGG AACAATGGAA GCAGACCGTG GGATGGAAGG CTTAATCAGT 
CCCACGGAGG CCGTGGGAAA CAGCTGTGGG GCTAGCAGCT CCTGTCCTGG CTGGCTTCGG 
AAGGAGCTGG AAAACGCAGA GTTCATTCCC ATGCCTGACA GCCCCGCTCC CTTAAGTGCG 
GCGTTTTCAC AGTCTGAGAA GGACACGCTG CCCTATGAAG AGCTGCAGGG ACTCAAGGTC 
GCATCTGAAG TTCCTCCGGA GCCCCAGCGT GCAGCAGGAG CCTGGCCCCC TCGGCTGGAT 
CCTGCAGTGC CGTGTGTTGG GAAAGCCCTG ACCTCTGCTG CTTGTGCGTG CAGTGCTCTG 
CAGGTGGAGG TTGACAGGTT GCAGGCTCTG GTGTTAAAGT GTCTGGAAGA ACAGCAGAAG 
CTCCAGCAAG AAAACCTCCA GATGTTTACC CAACTACAAA AGCTCAACAA GAAATTGGAA 
GGAGGCCAGC AGGTGGGGAT GCACTCCAGA GGAACCCAGA CAGCAAAGGA AGAAATGGAA 
ATGGATCCAA AGCCTGACTT AGACTCGGAG TCCTGGTGCC TCCTCGGAAC AGACTCTTGT 
CGACCCAGCC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nek9 ( XM_006515708.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515771.1 Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 9 (Nek9), transcript variant X1, mRNA.

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CloneID	OMu47735
Clone ID Related Accession (Same CDS sequence)	XM_006515708.4 , XM_006515708.3
Accession Version	XM_006515708.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase Nek9 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515708.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGTGG GCACTCCATA TTACATGTCT CCAGAGCTCT GCCAAGGAGT AAAATACAAT 
TTCAAGTCTG ATATATGGGC AGTTGGCTGT GTCATTTTTG AACTGCTTAC TCTGAAAAGA 
ACATTTGATG CTACAAACCC ACTCAACCTA TGTGTGAAGA TCGTGCAGGG AATCCGGGCC 
ATGGAAGTTG ACTCGAGTCA GTATTCTTTG GAACTGATCC AGTTGGTACA CGCATGCCTC 
GACCAGGATC CTGAGCAGAG GCCCGCTGCA GATGCACTCC TGGATCTCCC CCTTCTCAGG 
ACCCGCAGGA GAGAGATGGA GGAAAAAGTC ACTCTGCTCA ATGCACCTAC AAAGAGACCA 
AGGTCAAGCA CTGTGACGGA AGCACCCATT GCTGTGGTAA CATCACGAAC CAGTGAAGTC 
TATGTTTGGG GTGGTGGAAA ATCCACACCT CAGAAATTGG ACGTCATCAA GAGTGGCTGT 
AGTGCTCGAC AGGTGTGTGC GGGGAACACC CACTTTGCTG TGGTCACCGT GGAGAAGGAA 
CTATATACCT GGGTGAACAT GCAAGGGGGC ACTAAACTTC ACGGTCAGCT AGGACATGGA 
GACAAAGCTT CCTACCGACA GCCAAAGCAT GTAGAAAAGT TACAAGGGAA GGCTATCCAC 
CAGGTGTCGT GTGGGGATGA TTTCACTGTC TGTGTCACAG ATGAGGGTCA GCTCTACGCC 
TTTGGATCAG ATTATTATGG CTGCATGGGA GTGGACAAGG TTTCTGGCCC TGAAGTGCTA 
GAACCCATGC AGCTGAACTT CTTCCTCAGC AATCCTGTGG AGCAGGTCTC CTGTGGGGAT 
AATCATGTGG TAGTTCTGAC ACGAAACAAA GAAGTCTACT CTTGGGGCTG TGGTGAATAT 
GGACGGCTGG GTTTGGATTC AGAAGAGGAT TACTATACAC CACAGAGGGT GGATGTTCCA 
AAGGCCTTAA TCATTGTTGC AGTTCAATGT GGCTGTGATG GGACCTTTCT GCTCACTCAG 
TCAGGCAAAG TGCTGGCCTG TGGACTCAAT GAGTTCAACA AGCTGGGGCT GAATCAGTGC 
ATGTCTGGAA TCATCAACCA TGAAGCATAC CATGAAGTTC CCTACACAAC CTCCTTTACC 
TTGGCCAAAC AGTTGTCCTT TTACAAAATC CGTACCATTG CCCCAGGCAA GACTCACACA 
GCTGCTATTG ATGAACGAGG CCGGTTGCTG ACTTTTGGCT GTAATAAGTG TGGGCAGCTG 
GGTGTTGGGA ATTATAAGAA ACGCCTGGGA ATCAACCTGT TGGGGGGACC CCTTGGCGGG 
AAGCAAGTGA TTAGGGTCTC GTGTGGCGAT GAGTTCACCA TTGCTGCCAC CGACGATAAT 
CACATCTTTG CCTGGGGTAA TGGTGGTAAT GGCCGCCTGG CAATGACTCC CACAGAGAGA 
CCACATGGCT CTGATATCTG TACCTCATGG CCTCGGCCTA TTTTTGGATC CCTGCATCAT 
GTTCCAGACC TTTCTTGCCG TGGCTGGCAC ACCATTCTTA TTGTTGAGAA AGTGTTGAAT 
TCCAAGACCA TCCGTTCCAA TAGCAGTGGC CTGTCCATCG GAACTGTGGT TCAGAGCTCC 
AGCCCAGGAG GTGGCATTGG TGGTGGTGGT GGTGGTGGCG GCGGCGGCGG GGGTGAAGAG 
GAAGACAGTC AACAGGAATC TGAAACTCCA GATCCAAGCG GAGGCTTCCG AGGAACAATG 
GAAGCAGACC GTGGGATGGA AGGCTTAATC AGTCCCACGG AGGCCGTGGG AAACAGCTGT 
GGGGCTAGCA GCTCCTGTCC TGGCTGGCTT CGGAAGGAGC TGGAAAACGC AGAGTTCATT 
CCCATGCCTG ACAGCCCCGC TCCCTTAAGT GCGGCGTTTT CACAGTCTGA GAAGGACACG 
CTGCCCTATG AAGAGCTGCA GGGACTCAAG GTCGCATCTG AAGTTCCTCC GGAGCCCCAG 
CGTGCAGCAG GAGCCTGGCC CCCTCGGCTG GATCCTGCAG TGCCGTGTGT TGGGAAAGCC 
CTGACCTCTG CTGCTTGTGC GTGCAGTGCT CTGCAGGTGG AGGTTGACAG GTTGCAGGCT 
CTGGTGTTAA AGTGTCTGGA AGAACAGCAG AAGCTCCAGC AAGAAAACCT CCAGATGTTT 
ACCCAACTAC AAAAGCTCAA CAAGAAATTG GAAGGAGGCC AGCAGGTGGG GATGCACTCC 
AGAGGAACCC AGACAGCAAA GGAAGAAATG GAAATGGATC CAAAGCCTGA CTTAGACTCG 
GAGTCCTGGT GCCTCCTCGG AACAGACTCT TGTCGACCCA GCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515771.1
CDS	495..2822
Translation	MLVGTPYYMSPELCQGVKYNFKSDIWAVGCVIFELLTLKRTFDATNPLNLCVKIVQGIRAMEVDSSQYSLELIQLVHACLDQDPEQRPAADALLDLPLLRTRRREMEEKVTLLNAPTKRPRSSTVTEAPIAVVTSRTSEVYVWGGGKSTPQKLDVIKSGCSARQVCAGNTHFAVVTVEKELYTWVNMQGGTKLHGQLGHGDKASYRQPKHVEKLQGKAIHQVSCGDDFTVCVTDEGQLYAFGSDYYGCMGVDKVSGPEVLEPMQLNFFLSNPVEQVSCGDNHVVVLTRNKEVYSWGCGEYGRLGLDSEEDYYTPQRVDVPKALIIVAVQCGCDGTFLLTQSGKVLACGLNEFNKLGLNQCMSGIINHEAYHEVPYTTSFTLAKQLSFYKIRTIAPGKTHTAAIDERGRLLTFGCNKCGQLGVGNYKKRLGINLLGGPLGGKQVIRVSCGDEFTIAATDDNHIFAWGNGGNGRLAMTPTERPHGSDICTSWPRPIFGSLHHVPDLSCRGWHTILIVEKVLNSKTIRSNSSGLSIGTVVQSSSPGGGIGGGGGGGGGGGGEEEDSQQESETPDPSGGFRGTMEADRGMEGLISPTEAVGNSCGASSSCPGWLRKELENAEFIPMPDSPAPLSAAFSQSEKDTLPYEELQGLKVASEVPPEPQRAAGAWPPRLDPAVPCVGKALTSAACACSALQVEVDRLQALVLKCLEEQQKLQQENLQMFTQLQKLNKKLEGGQQVGMHSRGTQTAKEEMEMDPKPDLDSESWCLLGTDSCRPSL

Target ORF information:

RefSeq Version	XM_006515708.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 9 (Nek9), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515708.4

ORF Insert Sequence:

ATGCTTGTGG GCACTCCATA TTACATGTCT CCAGAGCTCT GCCAAGGAGT AAAATACAAT 
TTCAAGTCTG ATATATGGGC AGTTGGCTGT GTCATTTTTG AACTGCTTAC TCTGAAAAGA 
ACATTTGATG CTACAAACCC ACTCAACCTA TGTGTGAAGA TCGTGCAGGG AATCCGGGCC 
ATGGAAGTTG ACTCGAGTCA GTATTCTTTG GAACTGATCC AGTTGGTACA CGCATGCCTC 
GACCAGGATC CTGAGCAGAG GCCCGCTGCA GATGCACTCC TGGATCTCCC CCTTCTCAGG 
ACCCGCAGGA GAGAGATGGA GGAAAAAGTC ACTCTGCTCA ATGCACCTAC AAAGAGACCA 
AGGTCAAGCA CTGTGACGGA AGCACCCATT GCTGTGGTAA CATCACGAAC CAGTGAAGTC 
TATGTTTGGG GTGGTGGAAA ATCCACACCT CAGAAATTGG ACGTCATCAA GAGTGGCTGT 
AGTGCTCGAC AGGTGTGTGC GGGGAACACC CACTTTGCTG TGGTCACCGT GGAGAAGGAA 
CTATATACCT GGGTGAACAT GCAAGGGGGC ACTAAACTTC ACGGTCAGCT AGGACATGGA 
GACAAAGCTT CCTACCGACA GCCAAAGCAT GTAGAAAAGT TACAAGGGAA GGCTATCCAC 
CAGGTGTCGT GTGGGGATGA TTTCACTGTC TGTGTCACAG ATGAGGGTCA GCTCTACGCC 
TTTGGATCAG ATTATTATGG CTGCATGGGA GTGGACAAGG TTTCTGGCCC TGAAGTGCTA 
GAACCCATGC AGCTGAACTT CTTCCTCAGC AATCCTGTGG AGCAGGTCTC CTGTGGGGAT 
AATCATGTGG TAGTTCTGAC ACGAAACAAA GAAGTCTACT CTTGGGGCTG TGGTGAATAT 
GGACGGCTGG GTTTGGATTC AGAAGAGGAT TACTATACAC CACAGAGGGT GGATGTTCCA 
AAGGCCTTAA TCATTGTTGC AGTTCAATGT GGCTGTGATG GGACCTTTCT GCTCACTCAG 
TCAGGCAAAG TGCTGGCCTG TGGACTCAAT GAGTTCAACA AGCTGGGGCT GAATCAGTGC 
ATGTCTGGAA TCATCAACCA TGAAGCATAC CATGAAGTTC CCTACACAAC CTCCTTTACC 
TTGGCCAAAC AGTTGTCCTT TTACAAAATC CGTACCATTG CCCCAGGCAA GACTCACACA 
GCTGCTATTG ATGAACGAGG CCGGTTGCTG ACTTTTGGCT GTAATAAGTG TGGGCAGCTG 
GGTGTTGGGA ATTATAAGAA ACGCCTGGGA ATCAACCTGT TGGGGGGACC CCTTGGCGGG 
AAGCAAGTGA TTAGGGTCTC GTGTGGCGAT GAGTTCACCA TTGCTGCCAC CGACGATAAT 
CACATCTTTG CCTGGGGTAA TGGTGGTAAT GGCCGCCTGG CAATGACTCC CACAGAGAGA 
CCACATGGCT CTGATATCTG TACCTCATGG CCTCGGCCTA TTTTTGGATC CCTGCATCAT 
GTTCCAGACC TTTCTTGCCG TGGCTGGCAC ACCATTCTTA TTGTTGAGAA AGTGTTGAAT 
TCCAAGACCA TCCGTTCCAA TAGCAGTGGC CTGTCCATCG GAACTGTGGT TCAGAGCTCC 
AGCCCAGGAG GTGGCATTGG TGGTGGTGGT GGTGGTGGCG GCGGCGGCGG GGGTGAAGAG 
GAAGACAGTC AACAGGAATC TGAAACTCCA GATCCAAGCG GAGGCTTCCG AGGAACAATG 
GAAGCAGACC GTGGGATGGA AGGCTTAATC AGTCCCACGG AGGCCGTGGG AAACAGCTGT 
GGGGCTAGCA GCTCCTGTCC TGGCTGGCTT CGGAAGGAGC TGGAAAACGC AGAGTTCATT 
CCCATGCCTG ACAGCCCCGC TCCCTTAAGT GCGGCGTTTT CACAGTCTGA GAAGGACACG 
CTGCCCTATG AAGAGCTGCA GGGACTCAAG GTCGCATCTG AAGTTCCTCC GGAGCCCCAG 
CGTGCAGCAG GAGCCTGGCC CCCTCGGCTG GATCCTGCAG TGCCGTGTGT TGGGAAAGCC 
CTGACCTCTG CTGCTTGTGC GTGCAGTGCT CTGCAGGTGG AGGTTGACAG GTTGCAGGCT 
CTGGTGTTAA AGTGTCTGGA AGAACAGCAG AAGCTCCAGC AAGAAAACCT CCAGATGTTT 
ACCCAACTAC AAAAGCTCAA CAAGAAATTG GAAGGAGGCC AGCAGGTGGG GATGCACTCC 
AGAGGAACCC AGACAGCAAA GGAAGAAATG GAAATGGATC CAAAGCCTGA CTTAGACTCG 
GAGTCCTGGT GCCTCCTCGG AACAGACTCT TGTCGACCCA GCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A gemcitabine sensitivity screen identifies a role for NEK9 in the replication stress response.
Nucleic acids research42(18)11517-27(2014 Oct)
Smith SC,Petrova AV,Madden MZ,Wang H,Pan Y,Warren MD,Hardy CW,Liang D,Liu EA,Robinson MH,Rudra S,Wang J,Ehdaivand S,Torres MA,Wang Y,Yu DS

Nek9 regulates spindle organization and cell cycle progression during mouse oocyte meiosis and its location in early embryo mitosis.
Cell cycle (Georgetown, Tex.)11(23)4366-77(2012 Dec)
Yang SW,Gao C,Chen L,Song YL,Zhu JL,Qi ST,Jiang ZZ,Wang ZW,Lin F,Huang H,Xing FQ,Sun QY

Adenovirus E1A proteins direct subcellular redistribution of Nek9, a NimA-related kinase.
Journal of cellular physiology212(1)13-25(2007 Jul)
Pelka P,Scim? A,Mandalfino C,Joch M,Abdulla P,Whyte P

Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
DNA research : an international journal for rapid publication of reports on genes and genomes11(3)205-18(2004 Jun)
Okazaki N,F-Kikuno R,Ohara R,Inamoto S,Koseki H,Hiraoka S,Saga Y,Seino S,Nishimura M,Kaisho T,Hoshino K,Kitamura H,Nagase T,Ohara O,Koga H

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Nek9 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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