Gpr142 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gpr142
Entrez Gene ID	217302
Full Name	G protein-coupled receptor 142
Synonyms	PGR2
General protein information	Preferred Names G protein-coupled receptor 142 Names probable G-protein coupled receptor 142 G protein-coupled receptor PGR2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001346772.1	NP_001333701.1	probable G-protein coupled receptor 142 isoform 2
XM_006533066.2	XP_006533129.1	probable G-protein coupled receptor 142 isoform X1
NM_181749.1	NP_861414.1	probable G-protein coupled receptor 142 isoform 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GPR142	XP_540406.1
Bos taurus (cattle)	GPR142	XP_002696265.2
Mus musculus (house mouse)	Gpr142	NP_861414.1
Danio rerio (zebrafish)	LOC100000501	XP_001340684.2
Homo sapiens (human)	GPR142	NP_861455.1
Pan troglodytes (chimpanzee)	GPR142	XP_523704.2
Rattus norvegicus (Norway rat)	Gpr142	XP_006247763.1
Gallus gallus (chicken)	GPR142	XP_004946307.1
Xenopus tropicalis (tropical clawed frog)	gpr142	XP_002937638.2

Gene Ontology
Bibliography

Related articles in PubMed

GPR142 Controls Tryptophan-Induced Insulin and Incretin Hormone Secretion to Improve Glucose Metabolism.
Lin HV, Efanov AM, Fang X, Beavers LS, Wang X, Wang J, Gonzalez Valcarcel IC, Ma T
PloS one11(6)e0157298(2016)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Diabetic modifier QTLs identified in F2 intercrosses between Akita and A/J mice.
Takeshita S, Moritani M, Kunika K, Inoue H, Itakura M
Mammalian genome : official journal of the International Mammalian Genome Society17(9)927-40(2006 Sep)

Characterisation and differential expression of two very closely related G-protein-coupled receptors, GPR139 and GPR142, in mouse tissue and during mouse development.
S?sens U, Hermans-Borgmeyer I, Urny J, Schaller HC
Neuropharmacology50(4)512-20(2006 Mar)

Seven evolutionarily conserved human rhodopsin G protein-coupled receptors lacking close relatives.
Fredriksson R, H?glund PJ, Gloriam DE, Lagerstr?m MC, Schi?th HB
FEBS letters554(3)381-8(2003 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

these findings demonstrate GPR142 is a Tryptophan receptor critically required for insulin and incretin hormone regulation and suggest GPR142 agonists may be effective therapies that leverage amino acid sensing pathways for the treatment of type 2 diabetes.
Title: GPR142 Controls Tryptophan-Induced Insulin and Incretin Hormone Secretion to Improve Glucose Metabolism.

GPR142 mRNA showed a ubiquitous expression both in the brain and in various peripheral glands and organs in pre- and postnatal development.
Title: Characterisation and differential expression of two very closely related G-protein-coupled receptors, GPR139 and GPR142, in mouse tissue and during mouse development.

The following Gpr142 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gpr142 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu46149	NM_001346772.1 Latest version!	Mus musculus G protein-coupled receptor 142 (Gpr142), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu58096	NM_181749.1 Latest version!	Mus musculus G protein-coupled receptor 142 (Gpr142), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu46148	XM_006533066.2 Latest version!	Mus musculus G protein-coupled receptor 142 (Gpr142), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gpr142 ( NM_001346772.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001333701.1 Mus musculus G protein-coupled receptor 142 (Gpr142), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu46149
Clone ID Related Accession (Same CDS sequence)	NM_001346772.1
Accession Version	NM_001346772.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Mus musculus(house mouse)
Product	probable G-protein coupled receptor 142 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL663079.9 and AL669969.14. On Oct 30, 2016 this sequence version replaced XM_006533067.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AY957501.1 [ECO:0000332] ##Evidence-Data-END##

ATGCACCTGA ATTCAAATCC CAACAGCTAC ATCTGTGACG CCTACCAACA TGCTGATCTT 
CTATGGTCCT TGTCTCCTCA TGTGTTAACA AAAGCTGTGC AGCCACAAGT GACCCTGCTG 
CCTACAGTCA ATGGTAGTAA CCCAAGATAC GATGGTGTGG ATGGCCACTG GCCAGAGAGC 
CCAGAGAGGT CACCCTGTGT GGCTGGTATC ATACCTGTCA TCTATTACAG CGTCCTGCTG 
AGCCTGGGGC TGCCTGTCAA CGTCCTGACT TCAGTGGCAC TGGCCCGTCT TGCTGCCAGG 
ACCAGGAAAC CCTCCTATCA CTATCTCCTG GCACTCACGG CTTCAGATAT CGTCACCCAA 
GTGATCATCG TCTTCGTAGG CTTCCTCCTG CAGGGAGCTG TGCTGGCCCG CCAGGTGCCC 
CAGGCCGTGG TGCGCACAGC TAACATCCTG GAGTTTGCTG CCAATCACGC CTCAGTCTGG 
ATTGCAGTCT TGTTCACGGT GGATCGCTAC AATGCCCTGT GCCGCCCTCT ACGCCATCGG 
GCCACCTCGT CCCCAGGCCG GACCCACCGC GCTATTGCTG CTGTCATTGG CGTTACCCTC 
CTGACCGGCA TCCCATTCTA CTGGTGGCTG GATGTGTGGA GGGATGCAGA CCCTCCTAGC 
ACTATGGACA AACTCCTCAA GTGGGCTCAC TGCCTCATCG TCTACTTCAT TCCCTGCAAT 
GTTTTCCTGG TCACCAACTC TGCCATCATC CTTCGGCTAC GGAAGAGAGG CCAGAGAGGG 
CTGCGGCCAT TGGTGAGCAA GAGCACAGCC ATCCTCCTGG GTGTCACCTC CCTCTTCGCC 
CTCCTCTGGG CACCACGTAT TATTGTCATG CTGTACCATC TGTATGTGGC CCCTGTTCAC 
CGGGATTGGA GGGTCCACCT GGCCTTGGAC ATAGCCAACA TGTTGGCCAT GCTCAACACA 
GAGGTCAACT TTGGCCTCTA TTGCTTCATC AGCAAGACTT TCAGAGCCAC TGTCCGACAG 
GTCATCTGTG ATGTCCATAT GGCTTGCGCC TTAAAGTCAC AGCCAAAGCA AACGGTGGTG 
GAACTCATGC TGAAGTCCGT AGGGACAGAG CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333701.1
CDS	1..1116
Translation	MHLNSNPNSYICDAYQHADLLWSLSPHVLTKAVQPQVTLLPTVNGSNPRYDGVDGHWPESPERSPCVAGIIPVIYYSVLLSLGLPVNVLTSVALARLAARTRKPSYHYLLALTASDIVTQVIIVFVGFLLQGAVLARQVPQAVVRTANILEFAANHASVWIAVLFTVDRYNALCRPLRHRATSSPGRTHRAIAAVIGVTLLTGIPFYWWLDVWRDADPPSTMDKLLKWAHCLIVYFIPCNVFLVTNSAIILRLRKRGQRGLRPLVSKSTAILLGVTSLFALLWAPRIIVMLYHLYVAPVHRDWRVHLALDIANMLAMLNTEVNFGLYCFISKTFRATVRQVICDVHMACALKSQPKQTVVELMLKSVGTEL

Target ORF information:

RefSeq Version	NM_001346772.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus G protein-coupled receptor 142 (Gpr142), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346772.1

ORF Insert Sequence:

ATGCACCTGA ATTCAAATCC CAACAGCTAC ATCTGTGACG CCTACCAACA TGCTGATCTT 
CTATGGTCCT TGTCTCCTCA TGTGTTAACA AAAGCTGTGC AGCCACAAGT GACCCTGCTG 
CCTACAGTCA ATGGTAGTAA CCCAAGATAC GATGGTGTGG ATGGCCACTG GCCAGAGAGC 
CCAGAGAGGT CACCCTGTGT GGCTGGTATC ATACCTGTCA TCTATTACAG CGTCCTGCTG 
AGCCTGGGGC TGCCTGTCAA CGTCCTGACT TCAGTGGCAC TGGCCCGTCT TGCTGCCAGG 
ACCAGGAAAC CCTCCTATCA CTATCTCCTG GCACTCACGG CTTCAGATAT CGTCACCCAA 
GTGATCATCG TCTTCGTAGG CTTCCTCCTG CAGGGAGCTG TGCTGGCCCG CCAGGTGCCC 
CAGGCCGTGG TGCGCACAGC TAACATCCTG GAGTTTGCTG CCAATCACGC CTCAGTCTGG 
ATTGCAGTCT TGTTCACGGT GGATCGCTAC AATGCCCTGT GCCGCCCTCT ACGCCATCGG 
GCCACCTCGT CCCCAGGCCG GACCCACCGC GCTATTGCTG CTGTCATTGG CGTTACCCTC 
CTGACCGGCA TCCCATTCTA CTGGTGGCTG GATGTGTGGA GGGATGCAGA CCCTCCTAGC 
ACTATGGACA AACTCCTCAA GTGGGCTCAC TGCCTCATCG TCTACTTCAT TCCCTGCAAT 
GTTTTCCTGG TCACCAACTC TGCCATCATC CTTCGGCTAC GGAAGAGAGG CCAGAGAGGG 
CTGCGGCCAT TGGTGAGCAA GAGCACAGCC ATCCTCCTGG GTGTCACCTC CCTCTTCGCC 
CTCCTCTGGG CACCACGTAT TATTGTCATG CTGTACCATC TGTATGTGGC CCCTGTTCAC 
CGGGATTGGA GGGTCCACCT GGCCTTGGAC ATAGCCAACA TGTTGGCCAT GCTCAACACA 
GAGGTCAACT TTGGCCTCTA TTGCTTCATC AGCAAGACTT TCAGAGCCAC TGTCCGACAG 
GTCATCTGTG ATGTCCATAT GGCTTGCGCC TTAAAGTCAC AGCCAAAGCA AACGGTGGTG 
GAACTCATGC TGAAGTCCGT AGGGACAGAG CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpr142 ( NM_181749.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_861414.1 Mus musculus G protein-coupled receptor 142 (Gpr142), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu58096
Clone ID Related Accession (Same CDS sequence)	NM_181749.1
Accession Version	NM_181749.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Mus musculus(house mouse)
Product	probable G-protein coupled receptor 142 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY288428.1. ##Evidence-Data-START## Transcript exon combination :: AY288428.1 [ECO:0000332] ##Evidence-Data-END##

ATGCACCTGA ATTCAAATCC CAACAGCTAC ATCTGTGACG CCTACCAACA TGCTGATCTT 
CTATGGTCCT TGTCTCCTCA TGTGTTAACA AAAGCTGTGC AGCCACAAGT GACCCTGCTG 
CCTACAGTCA ATGGTAGTAA CCCAAGATAC GATGGTGTGG ATGGCCACTG GCCAGAGAGC 
CCAGAGAGGT CACCCTGTGT GGCTGGTATC ATACCTGTCA TCTATTACAG CGTCCTGCTG 
AGCCTGGGGC TGCCTGTGGC ACTGGCCCGT CTTGCTGCCA GGACCAGGAA ACCCTCCTAT 
CACTATCTCC TGGCACTCAC GGCTTCAGAT ATCGTCACCC AAGTGATCAT CGTCTTCGTA 
GGCTTCCTCC TGCAGGGAGC TGTGCTGGCC CGCCAGGTGC CCCAGGCCGT GGTGCGCACA 
GCTAACATCC TGGAGTTTGC TGCCAATCAC GCCTCAGTCT GGATTGCAGT CTTGTTCACG 
GTGGATCGCT ACAATGCCCT GTGCCGCCCT CTACGCCATC GGGCCACCTC GTCCCCAGGC 
CGGACCCACC GCGCTATTGC TGCTGTCATT GGCGTTACCC TCCTGACCGG CATCCCATTC 
TACTGGTGGC TGGATGTGTG GAGGGATGCA GACCCTCCTA GCACTATGGA CAAACTCCTC 
AAGTGGGCTC ACTGCCTCAT CGTCTACTTC ATTCCCTGCA ATGTTTTCCT GGTCACCAAC 
TCTGCCATCA TCCTTCGGCT ACGGAAGAGA GGCCAGAGAG GGCTGCGGCC ATTGGTGAGC 
AAGAGCACAG CCATCCTCCT GGGTGTCACC TCCCTCTTCG CCCTCCTCTG GGCACCACGT 
ATTATTGTCA TGCTGTACCA TCTGTATGTG GCCCCTGTTC ACCGGGATTG GAGGGTCCAC 
CTGGCCTTGG ACATAGCCAA CATGTTGGCC ATGCTCAACA CAGAGGTCAA CTTTGGCCTC 
TATTGCTTCA TCAGCAAGAC TTTCAGAGCC ACTGTCCGAC AGGTCATCTG TGATGTCCAT 
ATGGCTTGCG CCTTAAAGTC ACAGCCAAAG CAAACGGTGG TGGAACTCAT GCTGAAGTCC 
GTAGGGACAG AGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_861414.1
CDS	1..1098
Translation	MHLNSNPNSYICDAYQHADLLWSLSPHVLTKAVQPQVTLLPTVNGSNPRYDGVDGHWPESPERSPCVAGIIPVIYYSVLLSLGLPVALARLAARTRKPSYHYLLALTASDIVTQVIIVFVGFLLQGAVLARQVPQAVVRTANILEFAANHASVWIAVLFTVDRYNALCRPLRHRATSSPGRTHRAIAAVIGVTLLTGIPFYWWLDVWRDADPPSTMDKLLKWAHCLIVYFIPCNVFLVTNSAIILRLRKRGQRGLRPLVSKSTAILLGVTSLFALLWAPRIIVMLYHLYVAPVHRDWRVHLALDIANMLAMLNTEVNFGLYCFISKTFRATVRQVICDVHMACALKSQPKQTVVELMLKSVGTEL

Target ORF information:

RefSeq Version	NM_181749.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus G protein-coupled receptor 142 (Gpr142), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181749.1

ORF Insert Sequence:

ATGCACCTGA ATTCAAATCC CAACAGCTAC ATCTGTGACG CCTACCAACA TGCTGATCTT 
CTATGGTCCT TGTCTCCTCA TGTGTTAACA AAAGCTGTGC AGCCACAAGT GACCCTGCTG 
CCTACAGTCA ATGGTAGTAA CCCAAGATAC GATGGTGTGG ATGGCCACTG GCCAGAGAGC 
CCAGAGAGGT CACCCTGTGT GGCTGGTATC ATACCTGTCA TCTATTACAG CGTCCTGCTG 
AGCCTGGGGC TGCCTGTGGC ACTGGCCCGT CTTGCTGCCA GGACCAGGAA ACCCTCCTAT 
CACTATCTCC TGGCACTCAC GGCTTCAGAT ATCGTCACCC AAGTGATCAT CGTCTTCGTA 
GGCTTCCTCC TGCAGGGAGC TGTGCTGGCC CGCCAGGTGC CCCAGGCCGT GGTGCGCACA 
GCTAACATCC TGGAGTTTGC TGCCAATCAC GCCTCAGTCT GGATTGCAGT CTTGTTCACG 
GTGGATCGCT ACAATGCCCT GTGCCGCCCT CTACGCCATC GGGCCACCTC GTCCCCAGGC 
CGGACCCACC GCGCTATTGC TGCTGTCATT GGCGTTACCC TCCTGACCGG CATCCCATTC 
TACTGGTGGC TGGATGTGTG GAGGGATGCA GACCCTCCTA GCACTATGGA CAAACTCCTC 
AAGTGGGCTC ACTGCCTCAT CGTCTACTTC ATTCCCTGCA ATGTTTTCCT GGTCACCAAC 
TCTGCCATCA TCCTTCGGCT ACGGAAGAGA GGCCAGAGAG GGCTGCGGCC ATTGGTGAGC 
AAGAGCACAG CCATCCTCCT GGGTGTCACC TCCCTCTTCG CCCTCCTCTG GGCACCACGT 
ATTATTGTCA TGCTGTACCA TCTGTATGTG GCCCCTGTTC ACCGGGATTG GAGGGTCCAC 
CTGGCCTTGG ACATAGCCAA CATGTTGGCC ATGCTCAACA CAGAGGTCAA CTTTGGCCTC 
TATTGCTTCA TCAGCAAGAC TTTCAGAGCC ACTGTCCGAC AGGTCATCTG TGATGTCCAT 
ATGGCTTGCG CCTTAAAGTC ACAGCCAAAG CAAACGGTGG TGGAACTCAT GCTGAAGTCC 
GTAGGGACAG AGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpr142 ( XM_006533066.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533129.1 Mus musculus G protein-coupled receptor 142 (Gpr142), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu46148
Clone ID Related Accession (Same CDS sequence)	XM_006533066.2
Accession Version	XM_006533066.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1047bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	probable G-protein coupled receptor 142 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006533066.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACTTG AGGGAGCAGA GACAGCTGTG CAGCCACAAG TGACCCTGCT GCCTACAGTC 
AATGGTAGTA ACCCAAGATA CGATGGTGTG GATGGCCACT GGCCAGAGAG CCCAGAGAGG 
TCACCCTGTG TGGCTGGTAT CATACCTGTC ATCTATTACA GCGTCCTGCT GAGCCTGGGG 
CTGCCTGTCA ACGTCCTGAC TTCAGTGGCA CTGGCCCGTC TTGCTGCCAG GACCAGGAAA 
CCCTCCTATC ACTATCTCCT GGCACTCACG GCTTCAGATA TCGTCACCCA AGTGATCATC 
GTCTTCGTAG GCTTCCTCCT GCAGGGAGCT GTGCTGGCCC GCCAGGTGCC CCAGGCCGTG 
GTGCGCACAG CTAACATCCT GGAGTTTGCT GCCAATCACG CCTCAGTCTG GATTGCAGTC 
TTGTTCACGG TGGATCGCTA CAATGCCCTG TGCCGCCCTC TACGCCATCG GGCCACCTCG 
TCCCCAGGCC GGACCCACCG CGCTATTGCT GCTGTCATTG GCGTTACCCT CCTGACCGGC 
ATCCCATTCT ACTGGTGGCT GGATGTGTGG AGGGATGCAG ACCCTCCTAG CACTATGGAC 
AAACTCCTCA AGTGGGCTCA CTGCCTCATC GTCTACTTCA TTCCCTGCAA TGTTTTCCTG 
GTCACCAACT CTGCCATCAT CCTTCGGCTA CGGAAGAGAG GCCAGAGAGG GCTGCGGCCA 
TTGGTGAGCA AGAGCACAGC CATCCTCCTG GGTGTCACCT CCCTCTTCGC CCTCCTCTGG 
GCACCACGTA TTATTGTCAT GCTGTACCAT CTGTATGTGG CCCCTGTTCA CCGGGATTGG 
AGGGTCCACC TGGCCTTGGA CATAGCCAAC ATGTTGGCCA TGCTCAACAC AGAGGTCAAC 
TTTGGCCTCT ATTGCTTCAT CAGCAAGACT TTCAGAGCCA CTGTCCGACA GGTCATCTGT 
GATGTCCATA TGGCTTGCGC CTTAAAGTCA CAGCCAAAGC AAACGGTGGT GGAACTCATG 
CTGAAGTCCG TAGGGACAGA GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533129.1
CDS	136..1182
Translation	MGLEGAETAVQPQVTLLPTVNGSNPRYDGVDGHWPESPERSPCVAGIIPVIYYSVLLSLGLPVNVLTSVALARLAARTRKPSYHYLLALTASDIVTQVIIVFVGFLLQGAVLARQVPQAVVRTANILEFAANHASVWIAVLFTVDRYNALCRPLRHRATSSPGRTHRAIAAVIGVTLLTGIPFYWWLDVWRDADPPSTMDKLLKWAHCLIVYFIPCNVFLVTNSAIILRLRKRGQRGLRPLVSKSTAILLGVTSLFALLWAPRIIVMLYHLYVAPVHRDWRVHLALDIANMLAMLNTEVNFGLYCFISKTFRATVRQVICDVHMACALKSQPKQTVVELMLKSVGTEL

Target ORF information:

RefSeq Version	XM_006533066.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus G protein-coupled receptor 142 (Gpr142), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533066.2

ORF Insert Sequence:

ATGGGACTTG AGGGAGCAGA GACAGCTGTG CAGCCACAAG TGACCCTGCT GCCTACAGTC 
AATGGTAGTA ACCCAAGATA CGATGGTGTG GATGGCCACT GGCCAGAGAG CCCAGAGAGG 
TCACCCTGTG TGGCTGGTAT CATACCTGTC ATCTATTACA GCGTCCTGCT GAGCCTGGGG 
CTGCCTGTCA ACGTCCTGAC TTCAGTGGCA CTGGCCCGTC TTGCTGCCAG GACCAGGAAA 
CCCTCCTATC ACTATCTCCT GGCACTCACG GCTTCAGATA TCGTCACCCA AGTGATCATC 
GTCTTCGTAG GCTTCCTCCT GCAGGGAGCT GTGCTGGCCC GCCAGGTGCC CCAGGCCGTG 
GTGCGCACAG CTAACATCCT GGAGTTTGCT GCCAATCACG CCTCAGTCTG GATTGCAGTC 
TTGTTCACGG TGGATCGCTA CAATGCCCTG TGCCGCCCTC TACGCCATCG GGCCACCTCG 
TCCCCAGGCC GGACCCACCG CGCTATTGCT GCTGTCATTG GCGTTACCCT CCTGACCGGC 
ATCCCATTCT ACTGGTGGCT GGATGTGTGG AGGGATGCAG ACCCTCCTAG CACTATGGAC 
AAACTCCTCA AGTGGGCTCA CTGCCTCATC GTCTACTTCA TTCCCTGCAA TGTTTTCCTG 
GTCACCAACT CTGCCATCAT CCTTCGGCTA CGGAAGAGAG GCCAGAGAGG GCTGCGGCCA 
TTGGTGAGCA AGAGCACAGC CATCCTCCTG GGTGTCACCT CCCTCTTCGC CCTCCTCTGG 
GCACCACGTA TTATTGTCAT GCTGTACCAT CTGTATGTGG CCCCTGTTCA CCGGGATTGG 
AGGGTCCACC TGGCCTTGGA CATAGCCAAC ATGTTGGCCA TGCTCAACAC AGAGGTCAAC 
TTTGGCCTCT ATTGCTTCAT CAGCAAGACT TTCAGAGCCA CTGTCCGACA GGTCATCTGT 
GATGTCCATA TGGCTTGCGC CTTAAAGTCA CAGCCAAAGC AAACGGTGGT GGAACTCATG 
CTGAAGTCCG TAGGGACAGA GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

GPR142 Controls Tryptophan-Induced Insulin and Incretin Hormone Secretion to Improve Glucose Metabolism.
PloS one11(6)e0157298(2016)
Lin HV,Efanov AM,Fang X,Beavers LS,Wang X,Wang J,Gonzalez Valcarcel IC,Ma T

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Diabetic modifier QTLs identified in F2 intercrosses between Akita and A/J mice.
Mammalian genome : official journal of the International Mammalian Genome Society17(9)927-40(2006 Sep)
Takeshita S,Moritani M,Kunika K,Inoue H,Itakura M

Characterisation and differential expression of two very closely related G-protein-coupled receptors, GPR139 and GPR142, in mouse tissue and during mouse development.
Neuropharmacology50(4)512-20(2006 Mar)
S?sens U,Hermans-Borgmeyer I,Urny J,Schaller HC

Seven evolutionarily conserved human rhodopsin G protein-coupled receptors lacking close relatives.
FEBS letters554(3)381-8(2003 Nov)
Fredriksson R,H?glund PJ,Gloriam DE,Lagerstr?m MC,Schi?th HB