Trim25 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trim25
Entrez Gene ID	217069
Full Name	tripartite motif-containing 25
Synonyms	AA960166,AL022677,EFP,Zfp147
General protein information	Preferred Names tripartite motif-containing 25 Names E3 ubiquitin/ISG15 ligase TRIM25 RING-type E3 ubiquitin transferase RING-type E3 ubiquitin transferase TRIM25 estrogen-responsive finger protein tripartite motif protein 25 tripartite motif-containing protein 25 ubiquitin/ISG15-conjugating enzyme TRIM25 zinc finger protein 147
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 C

mRNA and Protein(s)

mRNA	Protein	Name
XM_017314476.2	XP_017169965.1	E3 ubiquitin/ISG15 ligase TRIM25 isoform X1
XM_017314476.1	XP_017169965.1	E3 ubiquitin/ISG15 ligase TRIM25 isoform X1
NM_009546.2	NP_033572.2	E3 ubiquitin/ISG15 ligase TRIM25

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	trim25	NP_956469.1
Homo sapiens (human)	TRIM25	NP_005073.2
	LOC712588	XP_001101634.1
Rattus norvegicus (Norway rat)	Trim25	NP_001009536.1
Gallus gallus (chicken)	TRIM25	XP_415653.4
Canis lupus familiaris (dog)	TRIM25	XP_548223.3
Bos taurus (cattle)	TRIM25	NP_001093806.1
Mus musculus (house mouse)	Trim25	NP_033572.2
Pan troglodytes (chimpanzee)	TRIM25	XP_511899.3

Gene Ontology
Bibliography

Related articles in PubMed

An Integrated Systems Biology Approach Identifies TRIM25 as a Key Determinant of Breast Cancer Metastasis.
Walsh LA, Alvarez MJ, Sabio EY, Reyngold M, Makarov V, Mukherjee S, Lee KW, Desrichard A, Turcan ?, Dalin MG, Rajasekhar VK, Chen S, Vahdat LT, Califano A, Chan TA
Cell reports20(7)1623-1640(2017 Aug)

Cyclophilin A-regulated ubiquitination is critical for RIG-I-mediated antiviral immune responses.
Liu W, Li J, Zheng W, Shang Y, Zhao Z, Wang S, Bi Y, Zhang S, Xu C, Duan Z, Zhang L, Wang YL, Jiang Z, Liu W, Sun L
eLife6(2017 Jun)

USP15 regulates type I interferon response and is required for pathogenesis of neuroinflammation.
Torre S, Polyak MJ, Langlais D, Fodil N, Kennedy JM, Radovanovic I, Berghout J, Leiva-Torres GA, Krawczyk CM, Ilangumaran S, Mossman K, Liang C, Knobeloch KP, Healy LM, Antel J, Arbour N, Prat A, Majewski J, Lathrop M, Vidal SM, Gros P
Nature immunology18(1)54-63(2017 Jan)

The role of Trim25 in development, disease and RNA metabolism.
Heikel G, Choudhury NR, Michlewski G
Biochemical Society transactions44(4)1045-50(2016 08)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Studies indicated that mice with disrupted Trim25 were viable and fertile, uterine response to oestrogen was greatly attenuated and the uteri were underdeveloped.
Title: The role of Trim25 in development, disease and RNA metabolism.

Two key residues (Asp(488) and Trp(621)) in the TRIM25 B30.2 domain are critical for binding to the RIG-I CARDs.
Title: Crystal structure of the TRIM25 B30.2 (PRYSPRY) domain: a key component of antiviral signalling.

Two well-known E3 ubiquitin ligases, Trim25 (also called Efp) and Trim71 (also called Lin41), are validated as RNA-binding proteins, revealing a potential link between RNA biology and protein-modification pathways.
Title: The RNA-binding protein repertoire of embryonic stem cells.

The following Trim25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trim25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu22068	NM_009546.2 Latest version!	Mus musculus tripartite motif-containing 25 (Trim25), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$370.30 $529.00
OMu77861	XM_017314476.2 Latest version!	Mus musculus tripartite motif-containing 25 (Trim25), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu77861	XM_017314476.1	Mus musculus tripartite motif-containing 25 (Trim25), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trim25 ( NM_009546.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033572.2 Mus musculus tripartite motif-containing 25 (Trim25), mRNA.

Price & Availability↑

CloneID	OMu22068
Clone ID Related Accession (Same CDS sequence)	NM_009546.2
Accession Version	NM_009546.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-25
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin/ISG15 ligase TRIM25
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK169562.1, AL646096.8 and BC028430.1. On Apr 13, 2007 this sequence version replaced NM_009546.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK154533.1, AK169562.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGAGC TGAATCCTCT GGCCGAGGAG CTGTCCTGCT CCGTCTGCCT GGAGCTCTTC 
AAGGAACCTG TCACCACTCC GTGCGGCCAC AATTTCTGCA TGTCATGCCT GGATGAGACG 
TGGGTCGTCC AGGGCCCGCC GTACCGATGC CCGCAATGTC GCAAAGTGTA CCAGGTGCGC 
CCGCAGCTGC AAAAGAACAC GGTAATGTGC GCGGTGGTGG AGCAGTTCCT GCAGGCCGAG 
CAGGCGCGGA CCCCGGTGGA CGACTGGACG CCTCCTGCCC GCTTCTCCGC CTCCAGCGCA 
GCCACCCAGG TGGCCTGCGA CCACTGCCTG ACGGAGATCG CAGTGAAGAC GTGCCTCGTG 
TGCATGGCCT CTTTCTGCCA GGAGCACTTG AGGCCTCACT TCGACAGTCC GGCCTTCCAG 
GATCACCCCC TACAGTCGCC CATTCGTGAC CTTCTGCGCC GCAAATGTAC CCAGCACAAC 
CGCCTTCGGG AATTGTTTTG TCCCGAGCAT GGCGAGTGCA TCTGCCATAT CTGCTTGGTA 
GAGCACAAGA CCTGCTCCCC TACGACCCTA AGTCAAGCCA GCGCCGACCT GGAGTACAAA 
CTGAGGAATA AACTCACTAT CATGCACAGC CACATCAATG GGGCAACCAA AGCATTGGAG 
GATGTGAGAT CCAAGCAACA GTGTGTGCAG GATTCTATGA AGAGGAAGAT GGAACAGCTG 
AGACAGGAGT ATATGGAAAT GAAGGCTGTC ATCGACGCAG CAGAGACCAG CTCCTTGCGC 
AAGTTAAAGG AGGAGGAGAA GAGAGTCTAC GGAAAATTCG ACACCATCTA CCAGGTTCTC 
GTCAAGAAGA AGAGTGAGAT GCAGAAACTG AAGGCAGAGG TTGAGCTCAT TATGGACAAA 
GGGGATGAGT TTGAGTTTCT GGAGAAAGCT GCAAAATTGC AAGGAGAGTC AACAAAACCA 
GTCTACATCC CTAAGATAGA TCTGGATCAT GATTTGATAA TGGGAATCTA CCAAGGGGCG 
GCGGACCTCA AGAGTGAACT GAAGCACTCC ATTAAGAAGC TACAGAAGAA GTCTGAGGAG 
CACAATGGCT CAGGTAACAA GGGAGACCAG ACACAGTCCA CTTTTAAACC CGTACAACCT 
TCGAAGAAGA CCATTCAAGA AAAGAAGACC AAGAAAACCC CTGTTGCTCC CGGCCCTCCC 
TCCCACTTCT CACCTAACAA GTTGCCCACC TTTGGAGCTC CTGGCCAATC ACTGGATTCA 
AAAGCAACTT CCCCTGATGC CGCACCCAAA GCCAGTGCTG CACAGCCAGA CTCTGTAGGA 
GTCAAGGCTA AGGTGCTAGA AAACTTCTTA ACCAAGTCCA GAACGGAGCT TCTGGAGTAT 
TTCGTGAAAG TCATCTTCGA CTACAATACC GCCCACAACA AAGTGTCTCT GTCAAACAAG 
TACACCACCG CCTCTGTGTC CGATGGGCTC CAGCACTACC GGTCCCATCC CCAGAGGTTC 
ACCTACTGCT CTCAGGTCCT GGGGCTGCAC TGCTACAAGA ACGGCATTCA CTACTGGGAG 
GTGGAACTAC AGAAGAACAA CTTCTGCGGC GTAGGCATCT GCTACGGCAG CATGGAACGG 
CAGGGCCCCG AGAGCCGGCT GGGCCGCAAC CCCAACTCAT GGTGTGTGGA GTGGTTCAAT 
AACAAGATCT CTGCCTGGCA CAACAACGTG GAGAAGACTC TGCCCTCCAC CAAGGCCACT 
CGAGTCGGCG TGCTGCTCAA CTGTGACCAC GGCTTCGTCA TTTTCTTTGC GGTCACCGAA 
AAGGTGCACC TGATGTACAA GTTCAAGGTG GACTTCACCG AAGCTCTGTA CCCAGCCTTC 
TGGGTGTTCT CGGCCGGTAC CACCCTCTCT ATCTGCTCCA AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033572.2
CDS	87..1991
Translation	MAELNPLAEELSCSVCLELFKEPVTTPCGHNFCMSCLDETWVVQGPPYRCPQCRKVYQVRPQLQKNTVMCAVVEQFLQAEQARTPVDDWTPPARFSASSAATQVACDHCLTEIAVKTCLVCMASFCQEHLRPHFDSPAFQDHPLQSPIRDLLRRKCTQHNRLRELFCPEHGECICHICLVEHKTCSPTTLSQASADLEYKLRNKLTIMHSHINGATKALEDVRSKQQCVQDSMKRKMEQLRQEYMEMKAVIDAAETSSLRKLKEEEKRVYGKFDTIYQVLVKKKSEMQKLKAEVELIMDKGDEFEFLEKAAKLQGESTKPVYIPKIDLDHDLIMGIYQGAADLKSELKHSIKKLQKKSEEHNGSGNKGDQTQSTFKPVQPSKKTIQEKKTKKTPVAPGPPSHFSPNKLPTFGAPGQSLDSKATSPDAAPKASAAQPDSVGVKAKVLENFLTKSRTELLEYFVKVIFDYNTAHNKVSLSNKYTTASVSDGLQHYRSHPQRFTYCSQVLGLHCYKNGIHYWEVELQKNNFCGVGICYGSMERQGPESRLGRNPNSWCVEWFNNKISAWHNNVEKTLPSTKATRVGVLLNCDHGFVIFFAVTEKVHLMYKFKVDFTEALYPAFWVFSAGTTLSICSK

Target ORF information:

RefSeq Version	NM_009546.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tripartite motif-containing 25 (Trim25), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009546.2

ORF Insert Sequence:

ATGGCGGAGC TGAATCCTCT GGCCGAGGAG CTGTCCTGCT CCGTCTGCCT GGAGCTCTTC 
AAGGAACCTG TCACCACTCC GTGCGGCCAC AATTTCTGCA TGTCATGCCT GGATGAGACG 
TGGGTCGTCC AGGGCCCGCC GTACCGATGC CCGCAATGTC GCAAAGTGTA CCAGGTGCGC 
CCGCAGCTGC AAAAGAACAC GGTAATGTGC GCGGTGGTGG AGCAGTTCCT GCAGGCCGAG 
CAGGCGCGGA CCCCGGTGGA CGACTGGACG CCTCCTGCCC GCTTCTCCGC CTCCAGCGCA 
GCCACCCAGG TGGCCTGCGA CCACTGCCTG ACGGAGATCG CAGTGAAGAC GTGCCTCGTG 
TGCATGGCCT CTTTCTGCCA GGAGCACTTG AGGCCTCACT TCGACAGTCC GGCCTTCCAG 
GATCACCCCC TACAGTCGCC CATTCGTGAC CTTCTGCGCC GCAAATGTAC CCAGCACAAC 
CGCCTTCGGG AATTGTTTTG TCCCGAGCAT GGCGAGTGCA TCTGCCATAT CTGCTTGGTA 
GAGCACAAGA CCTGCTCCCC TACGACCCTA AGTCAAGCCA GCGCCGACCT GGAGTACAAA 
CTGAGGAATA AACTCACTAT CATGCACAGC CACATCAATG GGGCAACCAA AGCATTGGAG 
GATGTGAGAT CCAAGCAACA GTGTGTGCAG GATTCTATGA AGAGGAAGAT GGAACAGCTG 
AGACAGGAGT ATATGGAAAT GAAGGCTGTC ATCGACGCAG CAGAGACCAG CTCCTTGCGC 
AAGTTAAAGG AGGAGGAGAA GAGAGTCTAC GGAAAATTCG ACACCATCTA CCAGGTTCTC 
GTCAAGAAGA AGAGTGAGAT GCAGAAACTG AAGGCAGAGG TTGAGCTCAT TATGGACAAA 
GGGGATGAGT TTGAGTTTCT GGAGAAAGCT GCAAAATTGC AAGGAGAGTC AACAAAACCA 
GTCTACATCC CTAAGATAGA TCTGGATCAT GATTTGATAA TGGGAATCTA CCAAGGGGCG 
GCGGACCTCA AGAGTGAACT GAAGCACTCC ATTAAGAAGC TACAGAAGAA GTCTGAGGAG 
CACAATGGCT CAGGTAACAA GGGAGACCAG ACACAGTCCA CTTTTAAACC CGTACAACCT 
TCGAAGAAGA CCATTCAAGA AAAGAAGACC AAGAAAACCC CTGTTGCTCC CGGCCCTCCC 
TCCCACTTCT CACCTAACAA GTTGCCCACC TTTGGAGCTC CTGGCCAATC ACTGGATTCA 
AAAGCAACTT CCCCTGATGC CGCACCCAAA GCCAGTGCTG CACAGCCAGA CTCTGTAGGA 
GTCAAGGCTA AGGTGCTAGA AAACTTCTTA ACCAAGTCCA GAACGGAGCT TCTGGAGTAT 
TTCGTGAAAG TCATCTTCGA CTACAATACC GCCCACAACA AAGTGTCTCT GTCAAACAAG 
TACACCACCG CCTCTGTGTC CGATGGGCTC CAGCACTACC GGTCCCATCC CCAGAGGTTC 
ACCTACTGCT CTCAGGTCCT GGGGCTGCAC TGCTACAAGA ACGGCATTCA CTACTGGGAG 
GTGGAACTAC AGAAGAACAA CTTCTGCGGC GTAGGCATCT GCTACGGCAG CATGGAACGG 
CAGGGCCCCG AGAGCCGGCT GGGCCGCAAC CCCAACTCAT GGTGTGTGGA GTGGTTCAAT 
AACAAGATCT CTGCCTGGCA CAACAACGTG GAGAAGACTC TGCCCTCCAC CAAGGCCACT 
CGAGTCGGCG TGCTGCTCAA CTGTGACCAC GGCTTCGTCA TTTTCTTTGC GGTCACCGAA 
AAGGTGCACC TGATGTACAA GTTCAAGGTG GACTTCACCG AAGCTCTGTA CCCAGCCTTC 
TGGGTGTTCT CGGCCGGTAC CACCCTCTCT ATCTGCTCCA AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trim25 ( XM_017314476.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169965.1 Mus musculus tripartite motif-containing 25 (Trim25), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu77861
Clone ID Related Accession (Same CDS sequence)	XM_017314476.2 , XM_017314476.1
Accession Version	XM_017314476.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1806bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin/ISG15 ligase TRIM25 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314476.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGC TGAATCCTCT GGCCGAGGAG CTGTCCTGCT CCGTCTGCCT GGAGCTCTTC 
AAGGAACCTG TCACCACTCC GTGCGGCCAC AATTTCTGCA TGTCATGCCT GGATGAGACG 
TGGGTCGTCC AGGGCCCGCC GTACCGATGC CCGCAATGTC GCAAAGTGTA CCAGGTGCGC 
CCGCAGCTGC AAAAGAACAC GGTAATGTGC GCGGTGGTGG AGCAGTTCCT GCAGGCCGAG 
CAGGCGCGGA CCCCGGTGGA CGACTGGACG CCTCCTGCCC GCTTCTCCGC CTCCAGCGCA 
GCCACCCAGG TGGCCTGCGA CCACTGCCTG ACGGAGATCG CAGTGAAGAC GTGCCTCGTG 
TGCATGGCCT CTTTCTGCCA GGAGCACTTG AGGCCTCACT TCGACAGTCC GGCCTTCCAG 
GATCACCCCC TACAGTCGCC CATTCGTGAC CTTCTGCGCC GCAAATGTAC CCAGCACAAC 
CGCCTTCGGG AATTGTTTTG TCCCGAGCAT GGCGAGTGCA TCTGCCATAT CTGCTTGGTA 
GAGCACAAGA CCTGCTCCCC TACGACCCTA AGTCAAGCCA GCGCCGACCT GGAGTACAAA 
CTGAGGAATA AACTCACTAT CATGCACAGC CACATCAATG GGGCAACCAA AGCATTGGAG 
GATGTGAGAT CCAAGCAACA GTGTGTGCAG GATTCTATGA AGAGGAAGAT GGAACAGCTG 
AGACAGGAGT ATATGGAAAT GAAGGCTGTC ATCGACGCAG CAGAGACCAG CTCCTTGCGC 
AAGTTAAAGG AGGAGGAGAA GAGAGTCTAC GGAAAATTCG ACACCATCTA CCAGGTTCTC 
GTCAAGAAGA AGAGTGAGAT GCAGAAACTG AAGGCAGAGG TTGAGCTCAT TATGGACAAA 
GGGGATGAGT TTGAGTTTCT GGAGAAAGCT GCAAAATTGC AAGGAGAGTC AACAAAACCA 
GTCTACATCC CTAAGATAGA TCTGGATCAT GATTTGATAA TGGGAATCTA CCAAGGGGCG 
GCGGACCTCA AGAGTGAACT GAAGCACTCC ATTAAGAAGC TACAGAAGAA GTCTGAGGAG 
CACAATGGCT CAGGTAACAA GGGAGACCAG ACACAGTCCA CTTTTAAACC CGTACAACCT 
TCGAAGAAGA CCATTCAAGA AAAGAAGACC AAGAAAACCC CCGCACCCAA AGCCAGTGCT 
GCACAGCCAG ACTCTGTAGG AGTCAAGGCT AAGGTGCTAG AAAACTTCTT AACCAAGTCC 
AGAACGGAGC TTCTGGAGTA TTTCGTGAAA GTCATCTTCG ACTACAATAC CGCCCACAAC 
AAAGTGTCTC TGTCAAACAA GTACACCACC GCCTCTGTGT CCGATGGGCT CCAGCACTAC 
CGGTCCCATC CCCAGAGGTT CACCTACTGC TCTCAGGTCC TGGGGCTGCA CTGCTACAAG 
AACGGCATTC ACTACTGGGA GGTGGAACTA CAGAAGAACA ACTTCTGCGG CGTAGGCATC 
TGCTACGGCA GCATGGAACG GCAGGGCCCC GAGAGCCGGC TGGGCCGCAA CCCCAACTCA 
TGGTGTGTGG AGTGGTTCAA TAACAAGATC TCTGCCTGGC ACAACAACGT GGAGAAGACT 
CTGCCCTCCA CCAAGGCCAC TCGAGTCGGC GTGCTGCTCA ACTGTGACCA CGGCTTCGTC 
ATTTTCTTTG CGGTCACCGA AAAGGTGCAC CTGATGTACA AGTTCAAGGT GGACTTCACC 
GAAGCTCTGT ACCCAGCCTT CTGGGTGTTC TCGGCCGGTA CCACCCTCTC TATCTGCTCC 
AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169965.1
CDS	87..1892
Translation	MAELNPLAEELSCSVCLELFKEPVTTPCGHNFCMSCLDETWVVQGPPYRCPQCRKVYQVRPQLQKNTVMCAVVEQFLQAEQARTPVDDWTPPARFSASSAATQVACDHCLTEIAVKTCLVCMASFCQEHLRPHFDSPAFQDHPLQSPIRDLLRRKCTQHNRLRELFCPEHGECICHICLVEHKTCSPTTLSQASADLEYKLRNKLTIMHSHINGATKALEDVRSKQQCVQDSMKRKMEQLRQEYMEMKAVIDAAETSSLRKLKEEEKRVYGKFDTIYQVLVKKKSEMQKLKAEVELIMDKGDEFEFLEKAAKLQGESTKPVYIPKIDLDHDLIMGIYQGAADLKSELKHSIKKLQKKSEEHNGSGNKGDQTQSTFKPVQPSKKTIQEKKTKKTPAPKASAAQPDSVGVKAKVLENFLTKSRTELLEYFVKVIFDYNTAHNKVSLSNKYTTASVSDGLQHYRSHPQRFTYCSQVLGLHCYKNGIHYWEVELQKNNFCGVGICYGSMERQGPESRLGRNPNSWCVEWFNNKISAWHNNVEKTLPSTKATRVGVLLNCDHGFVIFFAVTEKVHLMYKFKVDFTEALYPAFWVFSAGTTLSICSK

Target ORF information:

RefSeq Version	XM_017314476.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tripartite motif-containing 25 (Trim25), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314476.2

ORF Insert Sequence:

ATGGCGGAGC TGAATCCTCT GGCCGAGGAG CTGTCCTGCT CCGTCTGCCT GGAGCTCTTC 
AAGGAACCTG TCACCACTCC GTGCGGCCAC AATTTCTGCA TGTCATGCCT GGATGAGACG 
TGGGTCGTCC AGGGCCCGCC GTACCGATGC CCGCAATGTC GCAAAGTGTA CCAGGTGCGC 
CCGCAGCTGC AAAAGAACAC GGTAATGTGC GCGGTGGTGG AGCAGTTCCT GCAGGCCGAG 
CAGGCGCGGA CCCCGGTGGA CGACTGGACG CCTCCTGCCC GCTTCTCCGC CTCCAGCGCA 
GCCACCCAGG TGGCCTGCGA CCACTGCCTG ACGGAGATCG CAGTGAAGAC GTGCCTCGTG 
TGCATGGCCT CTTTCTGCCA GGAGCACTTG AGGCCTCACT TCGACAGTCC GGCCTTCCAG 
GATCACCCCC TACAGTCGCC CATTCGTGAC CTTCTGCGCC GCAAATGTAC CCAGCACAAC 
CGCCTTCGGG AATTGTTTTG TCCCGAGCAT GGCGAGTGCA TCTGCCATAT CTGCTTGGTA 
GAGCACAAGA CCTGCTCCCC TACGACCCTA AGTCAAGCCA GCGCCGACCT GGAGTACAAA 
CTGAGGAATA AACTCACTAT CATGCACAGC CACATCAATG GGGCAACCAA AGCATTGGAG 
GATGTGAGAT CCAAGCAACA GTGTGTGCAG GATTCTATGA AGAGGAAGAT GGAACAGCTG 
AGACAGGAGT ATATGGAAAT GAAGGCTGTC ATCGACGCAG CAGAGACCAG CTCCTTGCGC 
AAGTTAAAGG AGGAGGAGAA GAGAGTCTAC GGAAAATTCG ACACCATCTA CCAGGTTCTC 
GTCAAGAAGA AGAGTGAGAT GCAGAAACTG AAGGCAGAGG TTGAGCTCAT TATGGACAAA 
GGGGATGAGT TTGAGTTTCT GGAGAAAGCT GCAAAATTGC AAGGAGAGTC AACAAAACCA 
GTCTACATCC CTAAGATAGA TCTGGATCAT GATTTGATAA TGGGAATCTA CCAAGGGGCG 
GCGGACCTCA AGAGTGAACT GAAGCACTCC ATTAAGAAGC TACAGAAGAA GTCTGAGGAG 
CACAATGGCT CAGGTAACAA GGGAGACCAG ACACAGTCCA CTTTTAAACC CGTACAACCT 
TCGAAGAAGA CCATTCAAGA AAAGAAGACC AAGAAAACCC CCGCACCCAA AGCCAGTGCT 
GCACAGCCAG ACTCTGTAGG AGTCAAGGCT AAGGTGCTAG AAAACTTCTT AACCAAGTCC 
AGAACGGAGC TTCTGGAGTA TTTCGTGAAA GTCATCTTCG ACTACAATAC CGCCCACAAC 
AAAGTGTCTC TGTCAAACAA GTACACCACC GCCTCTGTGT CCGATGGGCT CCAGCACTAC 
CGGTCCCATC CCCAGAGGTT CACCTACTGC TCTCAGGTCC TGGGGCTGCA CTGCTACAAG 
AACGGCATTC ACTACTGGGA GGTGGAACTA CAGAAGAACA ACTTCTGCGG CGTAGGCATC 
TGCTACGGCA GCATGGAACG GCAGGGCCCC GAGAGCCGGC TGGGCCGCAA CCCCAACTCA 
TGGTGTGTGG AGTGGTTCAA TAACAAGATC TCTGCCTGGC ACAACAACGT GGAGAAGACT 
CTGCCCTCCA CCAAGGCCAC TCGAGTCGGC GTGCTGCTCA ACTGTGACCA CGGCTTCGTC 
ATTTTCTTTG CGGTCACCGA AAAGGTGCAC CTGATGTACA AGTTCAAGGT GGACTTCACC 
GAAGCTCTGT ACCCAGCCTT CTGGGTGTTC TCGGCCGGTA CCACCCTCTC TATCTGCTCC 
AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trim25 ( XM_017314476.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169965.1 Mus musculus tripartite motif-containing 25 (Trim25), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu77861
Clone ID Related Accession (Same CDS sequence)	XM_017314476.2 , XM_017314476.1
Accession Version	XM_017314476.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1806bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin/ISG15 ligase TRIM25 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGC TGAATCCTCT GGCCGAGGAG CTGTCCTGCT CCGTCTGCCT GGAGCTCTTC 
AAGGAACCTG TCACCACTCC GTGCGGCCAC AATTTCTGCA TGTCATGCCT GGATGAGACG 
TGGGTCGTCC AGGGCCCGCC GTACCGATGC CCGCAATGTC GCAAAGTGTA CCAGGTGCGC 
CCGCAGCTGC AAAAGAACAC GGTAATGTGC GCGGTGGTGG AGCAGTTCCT GCAGGCCGAG 
CAGGCGCGGA CCCCGGTGGA CGACTGGACG CCTCCTGCCC GCTTCTCCGC CTCCAGCGCA 
GCCACCCAGG TGGCCTGCGA CCACTGCCTG ACGGAGATCG CAGTGAAGAC GTGCCTCGTG 
TGCATGGCCT CTTTCTGCCA GGAGCACTTG AGGCCTCACT TCGACAGTCC GGCCTTCCAG 
GATCACCCCC TACAGTCGCC CATTCGTGAC CTTCTGCGCC GCAAATGTAC CCAGCACAAC 
CGCCTTCGGG AATTGTTTTG TCCCGAGCAT GGCGAGTGCA TCTGCCATAT CTGCTTGGTA 
GAGCACAAGA CCTGCTCCCC TACGACCCTA AGTCAAGCCA GCGCCGACCT GGAGTACAAA 
CTGAGGAATA AACTCACTAT CATGCACAGC CACATCAATG GGGCAACCAA AGCATTGGAG 
GATGTGAGAT CCAAGCAACA GTGTGTGCAG GATTCTATGA AGAGGAAGAT GGAACAGCTG 
AGACAGGAGT ATATGGAAAT GAAGGCTGTC ATCGACGCAG CAGAGACCAG CTCCTTGCGC 
AAGTTAAAGG AGGAGGAGAA GAGAGTCTAC GGAAAATTCG ACACCATCTA CCAGGTTCTC 
GTCAAGAAGA AGAGTGAGAT GCAGAAACTG AAGGCAGAGG TTGAGCTCAT TATGGACAAA 
GGGGATGAGT TTGAGTTTCT GGAGAAAGCT GCAAAATTGC AAGGAGAGTC AACAAAACCA 
GTCTACATCC CTAAGATAGA TCTGGATCAT GATTTGATAA TGGGAATCTA CCAAGGGGCG 
GCGGACCTCA AGAGTGAACT GAAGCACTCC ATTAAGAAGC TACAGAAGAA GTCTGAGGAG 
CACAATGGCT CAGGTAACAA GGGAGACCAG ACACAGTCCA CTTTTAAACC CGTACAACCT 
TCGAAGAAGA CCATTCAAGA AAAGAAGACC AAGAAAACCC CCGCACCCAA AGCCAGTGCT 
GCACAGCCAG ACTCTGTAGG AGTCAAGGCT AAGGTGCTAG AAAACTTCTT AACCAAGTCC 
AGAACGGAGC TTCTGGAGTA TTTCGTGAAA GTCATCTTCG ACTACAATAC CGCCCACAAC 
AAAGTGTCTC TGTCAAACAA GTACACCACC GCCTCTGTGT CCGATGGGCT CCAGCACTAC 
CGGTCCCATC CCCAGAGGTT CACCTACTGC TCTCAGGTCC TGGGGCTGCA CTGCTACAAG 
AACGGCATTC ACTACTGGGA GGTGGAACTA CAGAAGAACA ACTTCTGCGG CGTAGGCATC 
TGCTACGGCA GCATGGAACG GCAGGGCCCC GAGAGCCGGC TGGGCCGCAA CCCCAACTCA 
TGGTGTGTGG AGTGGTTCAA TAACAAGATC TCTGCCTGGC ACAACAACGT GGAGAAGACT 
CTGCCCTCCA CCAAGGCCAC TCGAGTCGGC GTGCTGCTCA ACTGTGACCA CGGCTTCGTC 
ATTTTCTTTG CGGTCACCGA AAAGGTGCAC CTGATGTACA AGTTCAAGGT GGACTTCACC 
GAAGCTCTGT ACCCAGCCTT CTGGGTGTTC TCGGCCGGTA CCACCCTCTC TATCTGCTCC 
AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169965.1
CDS	92..1897
Translation	MAELNPLAEELSCSVCLELFKEPVTTPCGHNFCMSCLDETWVVQGPPYRCPQCRKVYQVRPQLQKNTVMCAVVEQFLQAEQARTPVDDWTPPARFSASSAATQVACDHCLTEIAVKTCLVCMASFCQEHLRPHFDSPAFQDHPLQSPIRDLLRRKCTQHNRLRELFCPEHGECICHICLVEHKTCSPTTLSQASADLEYKLRNKLTIMHSHINGATKALEDVRSKQQCVQDSMKRKMEQLRQEYMEMKAVIDAAETSSLRKLKEEEKRVYGKFDTIYQVLVKKKSEMQKLKAEVELIMDKGDEFEFLEKAAKLQGESTKPVYIPKIDLDHDLIMGIYQGAADLKSELKHSIKKLQKKSEEHNGSGNKGDQTQSTFKPVQPSKKTIQEKKTKKTPAPKASAAQPDSVGVKAKVLENFLTKSRTELLEYFVKVIFDYNTAHNKVSLSNKYTTASVSDGLQHYRSHPQRFTYCSQVLGLHCYKNGIHYWEVELQKNNFCGVGICYGSMERQGPESRLGRNPNSWCVEWFNNKISAWHNNVEKTLPSTKATRVGVLLNCDHGFVIFFAVTEKVHLMYKFKVDFTEALYPAFWVFSAGTTLSICSK

Target ORF information:

RefSeq Version	XM_017314476.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tripartite motif-containing 25 (Trim25), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314476.1

ORF Insert Sequence:

ATGGCGGAGC TGAATCCTCT GGCCGAGGAG CTGTCCTGCT CCGTCTGCCT GGAGCTCTTC 
AAGGAACCTG TCACCACTCC GTGCGGCCAC AATTTCTGCA TGTCATGCCT GGATGAGACG 
TGGGTCGTCC AGGGCCCGCC GTACCGATGC CCGCAATGTC GCAAAGTGTA CCAGGTGCGC 
CCGCAGCTGC AAAAGAACAC GGTAATGTGC GCGGTGGTGG AGCAGTTCCT GCAGGCCGAG 
CAGGCGCGGA CCCCGGTGGA CGACTGGACG CCTCCTGCCC GCTTCTCCGC CTCCAGCGCA 
GCCACCCAGG TGGCCTGCGA CCACTGCCTG ACGGAGATCG CAGTGAAGAC GTGCCTCGTG 
TGCATGGCCT CTTTCTGCCA GGAGCACTTG AGGCCTCACT TCGACAGTCC GGCCTTCCAG 
GATCACCCCC TACAGTCGCC CATTCGTGAC CTTCTGCGCC GCAAATGTAC CCAGCACAAC 
CGCCTTCGGG AATTGTTTTG TCCCGAGCAT GGCGAGTGCA TCTGCCATAT CTGCTTGGTA 
GAGCACAAGA CCTGCTCCCC TACGACCCTA AGTCAAGCCA GCGCCGACCT GGAGTACAAA 
CTGAGGAATA AACTCACTAT CATGCACAGC CACATCAATG GGGCAACCAA AGCATTGGAG 
GATGTGAGAT CCAAGCAACA GTGTGTGCAG GATTCTATGA AGAGGAAGAT GGAACAGCTG 
AGACAGGAGT ATATGGAAAT GAAGGCTGTC ATCGACGCAG CAGAGACCAG CTCCTTGCGC 
AAGTTAAAGG AGGAGGAGAA GAGAGTCTAC GGAAAATTCG ACACCATCTA CCAGGTTCTC 
GTCAAGAAGA AGAGTGAGAT GCAGAAACTG AAGGCAGAGG TTGAGCTCAT TATGGACAAA 
GGGGATGAGT TTGAGTTTCT GGAGAAAGCT GCAAAATTGC AAGGAGAGTC AACAAAACCA 
GTCTACATCC CTAAGATAGA TCTGGATCAT GATTTGATAA TGGGAATCTA CCAAGGGGCG 
GCGGACCTCA AGAGTGAACT GAAGCACTCC ATTAAGAAGC TACAGAAGAA GTCTGAGGAG 
CACAATGGCT CAGGTAACAA GGGAGACCAG ACACAGTCCA CTTTTAAACC CGTACAACCT 
TCGAAGAAGA CCATTCAAGA AAAGAAGACC AAGAAAACCC CCGCACCCAA AGCCAGTGCT 
GCACAGCCAG ACTCTGTAGG AGTCAAGGCT AAGGTGCTAG AAAACTTCTT AACCAAGTCC 
AGAACGGAGC TTCTGGAGTA TTTCGTGAAA GTCATCTTCG ACTACAATAC CGCCCACAAC 
AAAGTGTCTC TGTCAAACAA GTACACCACC GCCTCTGTGT CCGATGGGCT CCAGCACTAC 
CGGTCCCATC CCCAGAGGTT CACCTACTGC TCTCAGGTCC TGGGGCTGCA CTGCTACAAG 
AACGGCATTC ACTACTGGGA GGTGGAACTA CAGAAGAACA ACTTCTGCGG CGTAGGCATC 
TGCTACGGCA GCATGGAACG GCAGGGCCCC GAGAGCCGGC TGGGCCGCAA CCCCAACTCA 
TGGTGTGTGG AGTGGTTCAA TAACAAGATC TCTGCCTGGC ACAACAACGT GGAGAAGACT 
CTGCCCTCCA CCAAGGCCAC TCGAGTCGGC GTGCTGCTCA ACTGTGACCA CGGCTTCGTC 
ATTTTCTTTG CGGTCACCGA AAAGGTGCAC CTGATGTACA AGTTCAAGGT GGACTTCACC 
GAAGCTCTGT ACCCAGCCTT CTGGGTGTTC TCGGCCGGTA CCACCCTCTC TATCTGCTCC 
AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An Integrated Systems Biology Approach Identifies TRIM25 as a Key Determinant of Breast Cancer Metastasis.
Cell reports20(7)1623-1640(2017 Aug)
Walsh LA,Alvarez MJ,Sabio EY,Reyngold M,Makarov V,Mukherjee S,Lee KW,Desrichard A,Turcan ?,Dalin MG,Rajasekhar VK,Chen S,Vahdat LT,Califano A,Chan TA

Cyclophilin A-regulated ubiquitination is critical for RIG-I-mediated antiviral immune responses.
eLife6(2017 Jun)
Liu W,Li J,Zheng W,Shang Y,Zhao Z,Wang S,Bi Y,Zhang S,Xu C,Duan Z,Zhang L,Wang YL,Jiang Z,Liu W,Sun L

USP15 regulates type I interferon response and is required for pathogenesis of neuroinflammation.
Nature immunology18(1)54-63(2017 Jan)
Torre S,Polyak MJ,Langlais D,Fodil N,Kennedy JM,Radovanovic I,Berghout J,Leiva-Torres GA,Krawczyk CM,Ilangumaran S,Mossman K,Liang C,Knobeloch KP,Healy LM,Antel J,Arbour N,Prat A,Majewski J,Lathrop M,Vidal SM,Gros P

The role of Trim25 in development, disease and RNA metabolism.
Biochemical Society transactions44(4)1045-50(2016 08)
Heikel G,Choudhury NR,Michlewski G

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Trim25 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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