Slfn1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slfn1
Entrez Gene ID	20555
Full Name	schlafen 1
Synonyms	AV316259
General protein information	Preferred Names schlafen 1 Names schlafen 1 OTTMUSCASG00059412 OTTMUSPWKG00059398 OTTMUSWSBG00059304 Schlafen family member 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 C\|11 50.3 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_011407.1	NP_035537.1	schlafen 1
NM_011407.2	NP_035537.1	schlafen 1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Slfn1	XP_001068751.2
Mus musculus (house mouse)	Slfn1	NP_035537.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Deletion of yes-associated protein (YAP) specifically in cardiac and vascular smooth muscle cells reveals a crucial role for YAP in mouse cardiovascular development.
Wang Y, Hu G, Liu F, Wang X, Wu M, Schwarz JJ, Zhou J
Circulation research114(6)957-65(2014 Mar)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Expression and regulatory effects of murine Schlafen (Slfn) genes in malignant melanoma and renal cell carcinoma.
Mavrommatis E, Arslan AD, Sassano A, Hua Y, Kroczynska B, Platanias LC
The Journal of biological chemistry288(46)33006-15(2013 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

several mouse Slfn genes are up-regulated in response to IFN treatment of mouse melanoma and renal cell carcinoma cells, including Slfn1, Slfn2, Slfn4, Slfn5, and Slfn8.
Title: Expression and regulatory effects of murine Schlafen (Slfn) genes in malignant melanoma and renal cell carcinoma.

Schlafen1 and Schlafen2 failed to inhibit cell proliferation, cause G1 cell cycle arrest, or affect Cyclin D1 level in murine fibroblasts.
Title: Lack of reproducible growth inhibition by Schlafen1 and Schlafen2 in vitro.

DnaJB6 is necessary for translocation of Slfn1 into the nucleus, where Slfn1 down-regulates cyclin D1, induces cell-cycle arrest and programmes a quiescent state of T-cells
Title: The Hsp40 family chaperone protein DnaJB6 enhances Schlafen1 nuclear localization which is critical for promotion of cell-cycle arrest in T-cells.

Results suggest that Schlafen-1 causes a cell cycle arrest during G1 by inhibiting induction of cyclin D1 by mitogens.
Title: Schlafen-1 causes a cell cycle arrest by inhibiting induction of cyclin D1.

The following Slfn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slfn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu08117	NM_011407.2 Latest version!	Mus musculus schlafen 1 (Slfn1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
View Old Accession Versions >>
OMu08117	NM_011407.1	Mus musculus schlafen 1 (Slfn1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slfn1 ( NM_011407.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035537.1 Mus musculus schlafen 1 (Slfn1), mRNA.

Price & Availability↑

CloneID	OMu08117
Clone ID Related Accession (Same CDS sequence)	NM_011407.1 , NM_011407.2
Accession Version	NM_011407.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-19
Organism	Mus musculus(house mouse)
Product	schlafen 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF099972.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF099972.1, BC052869.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGAACATCA CCGATGAAGG GACCCCTGTG CTGATTCTAA ATGCAGGAGG GATCACACTT 
GGGACAGAAA GTAGGAAAAC CATGGAGAAT CATGACAGGG TAGAAGAGAA CAGGAACATT 
ACAAAAGCTC TGTGTGCTCT GATTAATTCT GGAGAGGGAA AGGTCAAGGC TCACATTAAA 
AATCCAGACT ACATTTTGTC TAAACATGGA ATAGGAGAAG ATTTGGAAAC CTCTTTCAAG 
AACATTTTGC CATCTAGGCC CCTAGACTTT AAGCAATATC AATCCTACTT TTTCATCTGT 
GTAGAAAAAT CACAGAGTCC AGATGTCTCT GTTGGGAAGC CTGCCACCAT TGCAACCAAT 
TTGTACATGA GAAATGGGGC ATCATCAGTT GAAATGAACT TGGATGCTGC TCAGAAGTTC 
TTAGACAATA TCAAGGTTGC AGGAGGAAGG TCGCCTTCAG CAAGGCCTTC AGATAGGCCA 
GGCGATGATA CGCAAGAGGA AGGCCACATT CAGGAGTTGG CTGCTGCTTT TTTTAAACAG 
TCAAAACTTA CAAAGAAGGA AAATTTCTTA TTTAGTGAAT CCAAAAATGT TGAATATAAA 
TCCTTTGAAA CAAAAAAGTT GCTACAACGA GTTAAAGAGA TTCTCCCTCG AACTGTTTCT 
GCCTTTGCCA ACACTGACGG GGGATATTTG TTTATTGGTT TAGATGAAAA AAAACAGGAA 
ATAGTTGGTT TTGAAGCAAA GAATTGCCAG CCTAAGTGCT TAGAGAGTGA AATAGAAAAG 
TGCATCCAAC AGCTGCCTGT CACTCACTTC TGTGAGGAGA GGGAGAAGAT CAAGTACAAG 
TGCAAATTCA TCGAAGTCCA CGATTCCGGA GTTGTGTGTA AATATGTGTG TGCGCTCAGA 
GTGGAGAGGT TCTGCTGCGC AGTGTTTGCT GCAGAGCCCG AGTCCTGGCA CATGAAAGAT 
GGTGGTGTTA AAAGGTTTAC CATAGAGGAA TGGATCAAGC TCCTCATGTC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035537.1
CDS	258..1271
Translation	MNITDEGTPVLILNAGGITLGTESRKTMENHDRVEENRNITKALCALINSGEGKVKAHIKNPDYILSKHGIGEDLETSFKNILPSRPLDFKQYQSYFFICVEKSQSPDVSVGKPATIATNLYMRNGASSVEMNLDAAQKFLDNIKVAGGRSPSARPSDRPGDDTQEEGHIQELAAAFFKQSKLTKKENFLFSESKNVEYKSFETKKLLQRVKEILPRTVSAFANTDGGYLFIGLDEKKQEIVGFEAKNCQPKCLESEIEKCIQQLPVTHFCEEREKIKYKCKFIEVHDSGVVCKYVCALRVERFCCAVFAAEPESWHMKDGGVKRFTIEEWIKLLMS

Target ORF information:

RefSeq Version	NM_011407.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus schlafen 1 (Slfn1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011407.1

ORF Insert Sequence:

ATGAACATCA CCGATGAAGG GACCCCTGTG CTGATTCTAA ATGCAGGAGG GATCACACTT 
GGGACAGAAA GTAGGAAAAC CATGGAGAAT CATGACAGGG TAGAAGAGAA CAGGAACATT 
ACAAAAGCTC TGTGTGCTCT GATTAATTCT GGAGAGGGAA AGGTCAAGGC TCACATTAAA 
AATCCAGACT ACATTTTGTC TAAACATGGA ATAGGAGAAG ATTTGGAAAC CTCTTTCAAG 
AACATTTTGC CATCTAGGCC CCTAGACTTT AAGCAATATC AATCCTACTT TTTCATCTGT 
GTAGAAAAAT CACAGAGTCC AGATGTCTCT GTTGGGAAGC CTGCCACCAT TGCAACCAAT 
TTGTACATGA GAAATGGGGC ATCATCAGTT GAAATGAACT TGGATGCTGC TCAGAAGTTC 
TTAGACAATA TCAAGGTTGC AGGAGGAAGG TCGCCTTCAG CAAGGCCTTC AGATAGGCCA 
GGCGATGATA CGCAAGAGGA AGGCCACATT CAGGAGTTGG CTGCTGCTTT TTTTAAACAG 
TCAAAACTTA CAAAGAAGGA AAATTTCTTA TTTAGTGAAT CCAAAAATGT TGAATATAAA 
TCCTTTGAAA CAAAAAAGTT GCTACAACGA GTTAAAGAGA TTCTCCCTCG AACTGTTTCT 
GCCTTTGCCA ACACTGACGG GGGATATTTG TTTATTGGTT TAGATGAAAA AAAACAGGAA 
ATAGTTGGTT TTGAAGCAAA GAATTGCCAG CCTAAGTGCT TAGAGAGTGA AATAGAAAAG 
TGCATCCAAC AGCTGCCTGT CACTCACTTC TGTGAGGAGA GGGAGAAGAT CAAGTACAAG 
TGCAAATTCA TCGAAGTCCA CGATTCCGGA GTTGTGTGTA AATATGTGTG TGCGCTCAGA 
GTGGAGAGGT TCTGCTGCGC AGTGTTTGCT GCAGAGCCCG AGTCCTGGCA CATGAAAGAT 
GGTGGTGTTA AAAGGTTTAC CATAGAGGAA TGGATCAAGC TCCTCATGTC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slfn1 ( NM_011407.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035537.1 Mus musculus schlafen 1 (Slfn1), mRNA.

Price & Availability↑

CloneID	OMu08117
Clone ID Related Accession (Same CDS sequence)	NM_011407.1 , NM_011407.2
Accession Version	NM_011407.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-05
Organism	Mus musculus(house mouse)
Product	schlafen 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ056716.1, AF099972.1 and BX518009.1. On Feb 24, 2018 this sequence version replaced NM_011407.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC052869.1, AF099972.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACATCA CCGATGAAGG GACCCCTGTG CTGATTCTAA ATGCAGGAGG GATCACACTT 
GGGACAGAAA GTAGGAAAAC CATGGAGAAT CATGACAGGG TAGAAGAGAA CAGGAACATT 
ACAAAAGCTC TGTGTGCTCT GATTAATTCT GGAGAGGGAA AGGTCAAGGC TCACATTAAA 
AATCCAGACT ACATTTTGTC TAAACATGGA ATAGGAGAAG ATTTGGAAAC CTCTTTCAAG 
AACATTTTGC CATCTAGGCC CCTAGACTTT AAGCAATATC AATCCTACTT TTTCATCTGT 
GTAGAAAAAT CACAGAGTCC AGATGTCTCT GTTGGGAAGC CTGCCACCAT TGCAACCAAT 
TTGTACATGA GAAATGGGGC ATCATCAGTT GAAATGAACT TGGATGCTGC TCAGAAGTTC 
TTAGACAATA TCAAGGTTGC AGGAGGAAGG TCGCCTTCAG CAAGGCCTTC AGATAGGCCA 
GGCGATGATA CGCAAGAGGA AGGCCACATT CAGGAGTTGG CTGCTGCTTT TTTTAAACAG 
TCAAAACTTA CAAAGAAGGA AAATTTCTTA TTTAGTGAAT CCAAAAATGT TGAATATAAA 
TCCTTTGAAA CAAAAAAGTT GCTACAACGA GTTAAAGAGA TTCTCCCTCG AACTGTTTCT 
GCCTTTGCCA ACACTGACGG GGGATATTTG TTTATTGGTT TAGATGAAAA AAAACAGGAA 
ATAGTTGGTT TTGAAGCAAA GAATTGCCAG CCTAAGTGCT TAGAGAGTGA AATAGAAAAG 
TGCATCCAAC AGCTGCCTGT CACTCACTTC TGTGAGGAGA GGGAGAAGAT CAAGTACAAG 
TGCAAATTCA TCGAAGTCCA CGATTCCGGA GTTGTGTGTA AATATGTGTG TGCGCTCAGA 
GTGGAGAGGT TCTGCTGCGC AGTGTTTGCT GCAGAGCCCG AGTCCTGGCA CATGAAAGAT 
GGTGGTGTTA AAAGGTTTAC CATAGAGGAA TGGATCAAGC TCCTCATGTC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035537.1
CDS	283..1296
Translation	MNITDEGTPVLILNAGGITLGTESRKTMENHDRVEENRNITKALCALINSGEGKVKAHIKNPDYILSKHGIGEDLETSFKNILPSRPLDFKQYQSYFFICVEKSQSPDVSVGKPATIATNLYMRNGASSVEMNLDAAQKFLDNIKVAGGRSPSARPSDRPGDDTQEEGHIQELAAAFFKQSKLTKKENFLFSESKNVEYKSFETKKLLQRVKEILPRTVSAFANTDGGYLFIGLDEKKQEIVGFEAKNCQPKCLESEIEKCIQQLPVTHFCEEREKIKYKCKFIEVHDSGVVCKYVCALRVERFCCAVFAAEPESWHMKDGGVKRFTIEEWIKLLMS

Target ORF information:

RefSeq Version	NM_011407.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus schlafen 1 (Slfn1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011407.2

ORF Insert Sequence:

ATGAACATCA CCGATGAAGG GACCCCTGTG CTGATTCTAA ATGCAGGAGG GATCACACTT 
GGGACAGAAA GTAGGAAAAC CATGGAGAAT CATGACAGGG TAGAAGAGAA CAGGAACATT 
ACAAAAGCTC TGTGTGCTCT GATTAATTCT GGAGAGGGAA AGGTCAAGGC TCACATTAAA 
AATCCAGACT ACATTTTGTC TAAACATGGA ATAGGAGAAG ATTTGGAAAC CTCTTTCAAG 
AACATTTTGC CATCTAGGCC CCTAGACTTT AAGCAATATC AATCCTACTT TTTCATCTGT 
GTAGAAAAAT CACAGAGTCC AGATGTCTCT GTTGGGAAGC CTGCCACCAT TGCAACCAAT 
TTGTACATGA GAAATGGGGC ATCATCAGTT GAAATGAACT TGGATGCTGC TCAGAAGTTC 
TTAGACAATA TCAAGGTTGC AGGAGGAAGG TCGCCTTCAG CAAGGCCTTC AGATAGGCCA 
GGCGATGATA CGCAAGAGGA AGGCCACATT CAGGAGTTGG CTGCTGCTTT TTTTAAACAG 
TCAAAACTTA CAAAGAAGGA AAATTTCTTA TTTAGTGAAT CCAAAAATGT TGAATATAAA 
TCCTTTGAAA CAAAAAAGTT GCTACAACGA GTTAAAGAGA TTCTCCCTCG AACTGTTTCT 
GCCTTTGCCA ACACTGACGG GGGATATTTG TTTATTGGTT TAGATGAAAA AAAACAGGAA 
ATAGTTGGTT TTGAAGCAAA GAATTGCCAG CCTAAGTGCT TAGAGAGTGA AATAGAAAAG 
TGCATCCAAC AGCTGCCTGT CACTCACTTC TGTGAGGAGA GGGAGAAGAT CAAGTACAAG 
TGCAAATTCA TCGAAGTCCA CGATTCCGGA GTTGTGTGTA AATATGTGTG TGCGCTCAGA 
GTGGAGAGGT TCTGCTGCGC AGTGTTTGCT GCAGAGCCCG AGTCCTGGCA CATGAAAGAT 
GGTGGTGTTA AAAGGTTTAC CATAGAGGAA TGGATCAAGC TCCTCATGTC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Deletion of yes-associated protein (YAP) specifically in cardiac and vascular smooth muscle cells reveals a crucial role for YAP in mouse cardiovascular development.
Circulation research114(6)957-65(2014 Mar)
Wang Y,Hu G,Liu F,Wang X,Wu M,Schwarz JJ,Zhou J

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Expression and regulatory effects of murine Schlafen (Slfn) genes in malignant melanoma and renal cell carcinoma.
The Journal of biological chemistry288(46)33006-15(2013 Nov)
Mavrommatis E,Arslan AD,Sassano A,Hua Y,Kroczynska B,Platanias LC

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Slfn1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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