Sema4b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sema4b
Entrez Gene ID	20352
Full Name	sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B
Synonyms	SemC,Semac,mKIAA1745
General protein information	Preferred Names sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B Names semaphorin-4B sema C semaphorin-C
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 D2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001360135.1	NP_001347064.1	semaphorin-4B precursor
XM_030242259.1	XP_030098119.1	semaphorin-4B isoform X1
XM_011250830.2	XP_011249132.1	semaphorin-4B isoform X1
NM_013659.4	NP_038687.2	semaphorin-4B precursor
NM_001360135.2	NP_001347064.1	semaphorin-4B precursor
NM_013659.5	NP_038687.2	semaphorin-4B precursor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	SEMA4B	XP_004944013.1
Homo sapiens (human)	SEMA4B	NP_945119.1
Bos taurus (cattle)	SEMA4B	XP_005221915.1
	SEMA4B	XP_001096618.1
Rattus norvegicus (Norway rat)	Sema4b	NP_001163933.1
Canis lupus familiaris (dog)	SEMA4B	XP_545859.3
Mus musculus (house mouse)	Sema4b	NP_038687.2
Xenopus tropicalis (tropical clawed frog)	sema4b	XP_002932285.1
Pan troglodytes (chimpanzee)	SEMA4B	XP_510590.4

Gene Ontology
Bibliography

Related articles in PubMed

Astrogliosis Induced by Brain Injury Is Regulated by Sema4B Phosphorylation(123).
Ben-Gigi L, Sweetat S, Besser E, Fellig Y, Wiederhold T, Polakiewicz RD, Behar O
eNeuro2(3)(2015 May-Jun)

Semaphorin-Plexin Signaling Controls Mitotic Spindle Orientation during Epithelial Morphogenesis and Repair.
Xia J, Swiercz JM, Ba??n-Rodr?guez I, Matkovi? I, Federico G, Sun T, Franz T, Brakebusch CH, Kumanogoh A, Friedel RH, Mart?n-Belmonte F, Gr?ne HJ, Offermanns S, Worzfeld T
Developmental cell33(3)299-313(2015 May)

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Thompson CL, Ng L, Menon V, Martinez S, Lee CK, Glattfelder K, Sunkin SM, Henry A, Lau C, Dang C, Garcia-Lopez R, Martinez-Ferre A, Pombero A, Rubenstein JLR, Wakeman WB, Hohmann J, Dee N, Sodt AJ, Young R, Smith K, Nguyen TN, Kidney J, Kuan L, Jeromin A, Kaykas A, Miller J, Page D, Orta G, Bernard A, Riley Z, Smith S, Wohnoutka P, Hawrylycz MJ, Puelles L, Jones AR
Neuron83(2)309-323(2014 Jul)

An antagonistic interaction between PlexinB2 and Rnd3 controls RhoA activity and cortical neuron migration.
Azzarelli R, Pacary E, Garg R, Garcez P, van den Berg D, Riou P, Ridley AJ, Friedel RH, Parsons M, Guillemot F
Nature communications53405(2014 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Sema4B negatively regulates basophil-mediated T helper type (Th)2 cell and humoral memory responses.
Title: Identification of semaphorin 4B as a negative regulator of basophil-mediated immune responses.

Sema4B co-localizes with PSD-95 at synaptic contacts between cultured hippocampal neurons.
Title: Semaphorin 4B interacts with the post-synaptic density protein PSD-95/SAP90 and is recruited to synapses through a C-terminal PDZ-binding motif.

The following Sema4b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sema4b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu09748	XM_011250830.2 Latest version!	Mus musculus sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (Sema4b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu09748	NM_001360135.2 Latest version!	Mus musculus sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (Sema4b), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu09748	NM_013659.5 Latest version!	Mus musculus sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (Sema4b), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu89399	XM_030242259.1 Latest version!	Mus musculus sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (Sema4b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OMu09748	NM_001360135.1	Mus musculus sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (Sema4b), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu09748	NM_013659.4	Mus musculus sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (Sema4b), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sema4b ( NM_001360135.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001347064.1 Mus musculus sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (Sema4b), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu09748
Clone ID Related Accession (Same CDS sequence)	NM_001360135.1 , XM_011250830.2 , NM_013659.4 , NM_001360135.2 , NM_013659.5
Accession Version	NM_001360135.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Mus musculus(house mouse)
Product	semaphorin-4B precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC109232.17. On Jan 28, 2018 this sequence version replaced XM_011250830.2. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK134973.1, AK154969.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCCGGG CGAGCCGCTC CGCTGTCCTG CGGCGCGCGC TGCTGCTGCT GCTGCTACTG 
CTGCTGCTGC GGACGACGAC TACGCGGGCG CTCGGCCCCC GGATCAGTGT GCCGCTGGGC 
TCTGAAGAAA GGCTGATTAG AAAATTTGAA GCTGAAAACA TCTCCAACTA CACGGCCCTT 
CTGCTGAGCC AGGATGGCAA GACGCTGTAT GTGGGGGCCC GAGAGGCCCT CTTTGCACTT 
AACAGCAACC TCAGCTTCTT GCCAGGCGGG GAGTACCAAG AGCTGCTGTG GAGTGCAGAT 
GCTGACAGGA AGCAGCAGTG CAGCTTCAAG GGCAAGGACC CAAAGCGTGA CTGTCAAAAC 
TACATCAAGA TCCTCCTGCC ACTCAACAGC AGCCACCTGC TCACCTGTGG CACGGCCGCC 
TTCAGCCCCC TGTGTGCTTA CATTCACATA GCGAGCTTTA CTTTAGCCCA AGATGAGGCC 
GGCAATGTCA TTCTGGAGGA TGGCAAGGGT CGTTGTCCCT TTGACCCCAA CTTCAAGTCC 
ACGGCTCTGG TGGTTGATGG TGAGCTGTAC ACTGGAACAG TCAGTAGCTT CCAGGGAAAC 
GACCCAGCCA TTTCCCGGAG CCAGAGTTCC CGCCCCACCA AGACTGAGAG CTCCCTCAAC 
TGGCTACAAG ACCCTGCCTT TGTGGCCTCG GCCTACGTCC CCGAGAGCCT GGGCAGCCCC 
ATAGGTGATG ATGATAAGAT CTACTTCTTC TTCAGCGAGA CGGGCCAGGA GTTTGAGTTC 
TTTGAGAACA CCATCGTGTC CCGAGTTGCC CGAGTCTGTA AGGGCGATGA GGGTGGAGAG 
CGGGTGTTGC AGCAACGCTG GACCTCCTTT CTCAAGGCTC AGCTCCTGTG CTCCCGGCCT 
GATGATGGCT TTCCCTTTAA CGTGCTACAA GATGTCTTCA CCCTGAACCC CAACCCTCAG 
GATTGGCGCA AGACCCTTTT CTATGGGGTC TTTACCTCCC AGTGGCACAG AGGGACCACA 
GAAGGCTCTG CCATCTGCGT CTTCACCATG AATGATGTGC AGAAGGCCTT TGACGGCCTG 
TACAAGAAAG TAAACAGAGA GACACAGCAG TGGTATACCG AGACCCACCA GGTGCCCACA 
CCGCGGCCGG GAGCGTGCAT TACCAACAGT GCCCGGGAAC GGAAGATCAA CTCGTCCCTG 
CAGCTCCCAG ACCGAGTGCT GAACTTCCTC AAGGATCACT TCTTGATGGA TGGGCAGGTC 
CGCAGTCGCC TGCTGCTGCT GCAGCCCAGA GCCCGCTACC AGCGTGTGGC TGTGCACCGT 
GTGCCTGGCC TGCACAGCAC TTATGATGTC CTATTTCTGG GCACTGGTGA TGGCCGCCTG 
CACAAAGCAG TGACCCTGAG CTCCAGAGTC CACATCATTG AGGAGCTGCA GATCTTCCCT 
CAAGGACAGC CTGTGCAGAA CCTGCTCTTG GACAGCCATG GGGGACTGTT GTATGCCTCC 
TCCCATTCCG GGGTGGTGCA AGTGCCCGTA GCCAACTGCA GCCTGTACCC AACCTGTGGA 
GACTGCCTCC TGGCTCGAGA CCCCTACTGC GCCTGGACTG GCTCTGCCTG CAGGCTCGCT 
AGCCTCTACC AGCCTGATCT GGCCTCCAGG CCATGGACCC AGGACATTGA GGGTGCCAGT 
GTCAAGGAAC TCTGCAAGAA TTCCTCATAC AAGGCCCGGT TTCTTGTGCC AGGTAAGCCA 
TGTAAACAAG TCCAGATCCA ACCAAACACA GTGAACACCC TGGCCTGCCC ACTCCTCTCA 
AACCTGGCCA CTCGGCTCTG GGTGCACAAT GGAGCCCCAG TCAATGCCTC TGCCTCCTGC 
CGCGTGTTAC CCACCGGGGA CCTGCTGCTG GTGGGCAGCC AGCAGGGTTT GGGGGTGTTC 
CAGTGTTGGT CGATAGAAGA AGGATTCCAG CAGCTTGTGG CCAGCTACTG CCCAGAGGTG 
ATGGAGGAGG GGGTAATGGA CCAAAAGAAC CAGCGTGATG GTACCCCAGT CATTATCAAC 
ACATCACGAG TGAGTGCACC GGCTGGTGGC AGGGCCAGCT GGGGTGCGGA CAAGTCCTAC 
TGGAATGAAT TCCTGGTGAT GTGTACTCTG TTTGTGTTTG CTATGGTGCT TTTGTTTCTG 
TTCTTTCTCT ACCGACATCG GGATGGCATG AAACTCTTCC TAAAGCAGGG CGAATGTGCC 
AGTGTGCACC CCAAGACTCG CCCTATAGTG CTACCACCTG AGACCCGACC GCTGAATGGT 
GTCGGCCCTC CTAGCACCCC ACTTGACCAC CGAGGCTACC AGGCTCTGTC GGATAGCTCC 
CCAGGGCCCA GAGTCTTCAC TGAATCAGAG AAGAGGCCAC TGAGCATCCA GGACAGCTTT 
GTAGAGGTGT CTCCCGTGTG TCCCCGGCCC CGAGTTCGAC TGGGCTCTGA GATCCGAGAC 
TCTGTGGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001347064.1
CDS	213..2684
Translation	MGRASRSAVLRRALLLLLLLLLLRTTTTRALGPRISVPLGSEERLIRKFEAENISNYTALLLSQDGKTLYVGAREALFALNSNLSFLPGGEYQELLWSADADRKQQCSFKGKDPKRDCQNYIKILLPLNSSHLLTCGTAAFSPLCAYIHIASFTLAQDEAGNVILEDGKGRCPFDPNFKSTALVVDGELYTGTVSSFQGNDPAISRSQSSRPTKTESSLNWLQDPAFVASAYVPESLGSPIGDDDKIYFFFSETGQEFEFFENTIVSRVARVCKGDEGGERVLQQRWTSFLKAQLLCSRPDDGFPFNVLQDVFTLNPNPQDWRKTLFYGVFTSQWHRGTTEGSAICVFTMNDVQKAFDGLYKKVNRETQQWYTETHQVPTPRPGACITNSARERKINSSLQLPDRVLNFLKDHFLMDGQVRSRLLLLQPRARYQRVAVHRVPGLHSTYDVLFLGTGDGRLHKAVTLSSRVHIIEELQIFPQGQPVQNLLLDSHGGLLYASSHSGVVQVPVANCSLYPTCGDCLLARDPYCAWTGSACRLASLYQPDLASRPWTQDIEGASVKELCKNSSYKARFLVPGKPCKQVQIQPNTVNTLACPLLSNLATRLWVHNGAPVNASASCRVLPTGDLLLVGSQQGLGVFQCWSIEEGFQQLVASYCPEVMEEGVMDQKNQRDGTPVIINTSRVSAPAGGRASWGADKSYWNEFLVMCTLFVFAMVLLFLFFLYRHRDGMKLFLKQGECASVHPKTRPIVLPPETRPLNGVGPPSTPLDHRGYQALSDSSPGPRVFTESEKRPLSIQDSFVEVSPVCPRPRVRLGSEIRDSVV

Target ORF information:

RefSeq Version	NM_001360135.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (Sema4b), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001360135.1

ORF Insert Sequence:

ATGGGCCGGG CGAGCCGCTC CGCTGTCCTG CGGCGCGCGC TGCTGCTGCT GCTGCTACTG 
CTGCTGCTGC GGACGACGAC TACGCGGGCG CTCGGCCCCC GGATCAGTGT GCCGCTGGGC 
TCTGAAGAAA GGCTGATTAG AAAATTTGAA GCTGAAAACA TCTCCAACTA CACGGCCCTT 
CTGCTGAGCC AGGATGGCAA GACGCTGTAT GTGGGGGCCC GAGAGGCCCT CTTTGCACTT 
AACAGCAACC TCAGCTTCTT GCCAGGCGGG GAGTACCAAG AGCTGCTGTG GAGTGCAGAT 
GCTGACAGGA AGCAGCAGTG CAGCTTCAAG GGCAAGGACC CAAAGCGTGA CTGTCAAAAC 
TACATCAAGA TCCTCCTGCC ACTCAACAGC AGCCACCTGC TCACCTGTGG CACGGCCGCC 
TTCAGCCCCC TGTGTGCTTA CATTCACATA GCGAGCTTTA CTTTAGCCCA AGATGAGGCC 
GGCAATGTCA TTCTGGAGGA TGGCAAGGGT CGTTGTCCCT TTGACCCCAA CTTCAAGTCC 
ACGGCTCTGG TGGTTGATGG TGAGCTGTAC ACTGGAACAG TCAGTAGCTT CCAGGGAAAC 
GACCCAGCCA TTTCCCGGAG CCAGAGTTCC CGCCCCACCA AGACTGAGAG CTCCCTCAAC 
TGGCTACAAG ACCCTGCCTT TGTGGCCTCG GCCTACGTCC CCGAGAGCCT GGGCAGCCCC 
ATAGGTGATG ATGATAAGAT CTACTTCTTC TTCAGCGAGA CGGGCCAGGA GTTTGAGTTC 
TTTGAGAACA CCATCGTGTC CCGAGTTGCC CGAGTCTGTA AGGGCGATGA GGGTGGAGAG 
CGGGTGTTGC AGCAACGCTG GACCTCCTTT CTCAAGGCTC AGCTCCTGTG CTCCCGGCCT 
GATGATGGCT TTCCCTTTAA CGTGCTACAA GATGTCTTCA CCCTGAACCC CAACCCTCAG 
GATTGGCGCA AGACCCTTTT CTATGGGGTC TTTACCTCCC AGTGGCACAG AGGGACCACA 
GAAGGCTCTG CCATCTGCGT CTTCACCATG AATGATGTGC AGAAGGCCTT TGACGGCCTG 
TACAAGAAAG TAAACAGAGA GACACAGCAG TGGTATACCG AGACCCACCA GGTGCCCACA 
CCGCGGCCGG GAGCGTGCAT TACCAACAGT GCCCGGGAAC GGAAGATCAA CTCGTCCCTG 
CAGCTCCCAG ACCGAGTGCT GAACTTCCTC AAGGATCACT TCTTGATGGA TGGGCAGGTC 
CGCAGTCGCC TGCTGCTGCT GCAGCCCAGA GCCCGCTACC AGCGTGTGGC TGTGCACCGT 
GTGCCTGGCC TGCACAGCAC TTATGATGTC CTATTTCTGG GCACTGGTGA TGGCCGCCTG 
CACAAAGCAG TGACCCTGAG CTCCAGAGTC CACATCATTG AGGAGCTGCA GATCTTCCCT 
CAAGGACAGC CTGTGCAGAA CCTGCTCTTG GACAGCCATG GGGGACTGTT GTATGCCTCC 
TCCCATTCCG GGGTGGTGCA AGTGCCCGTA GCCAACTGCA GCCTGTACCC AACCTGTGGA 
GACTGCCTCC TGGCTCGAGA CCCCTACTGC GCCTGGACTG GCTCTGCCTG CAGGCTCGCT 
AGCCTCTACC AGCCTGATCT GGCCTCCAGG CCATGGACCC AGGACATTGA GGGTGCCAGT 
GTCAAGGAAC TCTGCAAGAA TTCCTCATAC AAGGCCCGGT TTCTTGTGCC AGGTAAGCCA 
TGTAAACAAG TCCAGATCCA ACCAAACACA GTGAACACCC TGGCCTGCCC ACTCCTCTCA 
AACCTGGCCA CTCGGCTCTG GGTGCACAAT GGAGCCCCAG TCAATGCCTC TGCCTCCTGC 
CGCGTGTTAC CCACCGGGGA CCTGCTGCTG GTGGGCAGCC AGCAGGGTTT GGGGGTGTTC 
CAGTGTTGGT CGATAGAAGA AGGATTCCAG CAGCTTGTGG CCAGCTACTG CCCAGAGGTG 
ATGGAGGAGG GGGTAATGGA CCAAAAGAAC CAGCGTGATG GTACCCCAGT CATTATCAAC 
ACATCACGAG TGAGTGCACC GGCTGGTGGC AGGGCCAGCT GGGGTGCGGA CAAGTCCTAC 
TGGAATGAAT TCCTGGTGAT GTGTACTCTG TTTGTGTTTG CTATGGTGCT TTTGTTTCTG 
TTCTTTCTCT ACCGACATCG GGATGGCATG AAACTCTTCC TAAAGCAGGG CGAATGTGCC 
AGTGTGCACC CCAAGACTCG CCCTATAGTG CTACCACCTG AGACCCGACC GCTGAATGGT 
GTCGGCCCTC CTAGCACCCC ACTTGACCAC CGAGGCTACC AGGCTCTGTC GGATAGCTCC 
CCAGGGCCCA GAGTCTTCAC TGAATCAGAG AAGAGGCCAC TGAGCATCCA GGACAGCTTT 
GTAGAGGTGT CTCCCGTGTG TCCCCGGCCC CGAGTTCGAC TGGGCTCTGA GATCCGAGAC 
TCTGTGGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema4b ( NM_013659.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038687.2 Mus musculus sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (Sema4b), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu09748
Clone ID Related Accession (Same CDS sequence)	NM_001360135.1 , XM_011250830.2 , NM_013659.4 , NM_001360135.2 , NM_013659.5
Accession Version	NM_013659.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Mus musculus(house mouse)
Product	semaphorin-4B precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK145570.1 and AC109232.17. On Jul 28, 2009 this sequence version replaced NM_013659.3. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK145570.1, AK030331.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCCGGG CGAGCCGCTC CGCTGTCCTG CGGCGCGCGC TGCTGCTGCT GCTGCTACTG 
CTGCTGCTGC GGACGACGAC TACGCGGGCG CTCGGCCCCC GGATCAGTGT GCCGCTGGGC 
TCTGAAGAAA GGCTGATTAG AAAATTTGAA GCTGAAAACA TCTCCAACTA CACGGCCCTT 
CTGCTGAGCC AGGATGGCAA GACGCTGTAT GTGGGGGCCC GAGAGGCCCT CTTTGCACTT 
AACAGCAACC TCAGCTTCTT GCCAGGCGGG GAGTACCAAG AGCTGCTGTG GAGTGCAGAT 
GCTGACAGGA AGCAGCAGTG CAGCTTCAAG GGCAAGGACC CAAAGCGTGA CTGTCAAAAC 
TACATCAAGA TCCTCCTGCC ACTCAACAGC AGCCACCTGC TCACCTGTGG CACGGCCGCC 
TTCAGCCCCC TGTGTGCTTA CATTCACATA GCGAGCTTTA CTTTAGCCCA AGATGAGGCC 
GGCAATGTCA TTCTGGAGGA TGGCAAGGGT CGTTGTCCCT TTGACCCCAA CTTCAAGTCC 
ACGGCTCTGG TGGTTGATGG TGAGCTGTAC ACTGGAACAG TCAGTAGCTT CCAGGGAAAC 
GACCCAGCCA TTTCCCGGAG CCAGAGTTCC CGCCCCACCA AGACTGAGAG CTCCCTCAAC 
TGGCTACAAG ACCCTGCCTT TGTGGCCTCG GCCTACGTCC CCGAGAGCCT GGGCAGCCCC 
ATAGGTGATG ATGATAAGAT CTACTTCTTC TTCAGCGAGA CGGGCCAGGA GTTTGAGTTC 
TTTGAGAACA CCATCGTGTC CCGAGTTGCC CGAGTCTGTA AGGGCGATGA GGGTGGAGAG 
CGGGTGTTGC AGCAACGCTG GACCTCCTTT CTCAAGGCTC AGCTCCTGTG CTCCCGGCCT 
GATGATGGCT TTCCCTTTAA CGTGCTACAA GATGTCTTCA CCCTGAACCC CAACCCTCAG 
GATTGGCGCA AGACCCTTTT CTATGGGGTC TTTACCTCCC AGTGGCACAG AGGGACCACA 
GAAGGCTCTG CCATCTGCGT CTTCACCATG AATGATGTGC AGAAGGCCTT TGACGGCCTG 
TACAAGAAAG TAAACAGAGA GACACAGCAG TGGTATACCG AGACCCACCA GGTGCCCACA 
CCGCGGCCGG GAGCGTGCAT TACCAACAGT GCCCGGGAAC GGAAGATCAA CTCGTCCCTG 
CAGCTCCCAG ACCGAGTGCT GAACTTCCTC AAGGATCACT TCTTGATGGA TGGGCAGGTC 
CGCAGTCGCC TGCTGCTGCT GCAGCCCAGA GCCCGCTACC AGCGTGTGGC TGTGCACCGT 
GTGCCTGGCC TGCACAGCAC TTATGATGTC CTATTTCTGG GCACTGGTGA TGGCCGCCTG 
CACAAAGCAG TGACCCTGAG CTCCAGAGTC CACATCATTG AGGAGCTGCA GATCTTCCCT 
CAAGGACAGC CTGTGCAGAA CCTGCTCTTG GACAGCCATG GGGGACTGTT GTATGCCTCC 
TCCCATTCCG GGGTGGTGCA AGTGCCCGTA GCCAACTGCA GCCTGTACCC AACCTGTGGA 
GACTGCCTCC TGGCTCGAGA CCCCTACTGC GCCTGGACTG GCTCTGCCTG CAGGCTCGCT 
AGCCTCTACC AGCCTGATCT GGCCTCCAGG CCATGGACCC AGGACATTGA GGGTGCCAGT 
GTCAAGGAAC TCTGCAAGAA TTCCTCATAC AAGGCCCGGT TTCTTGTGCC AGGTAAGCCA 
TGTAAACAAG TCCAGATCCA ACCAAACACA GTGAACACCC TGGCCTGCCC ACTCCTCTCA 
AACCTGGCCA CTCGGCTCTG GGTGCACAAT GGAGCCCCAG TCAATGCCTC TGCCTCCTGC 
CGCGTGTTAC CCACCGGGGA CCTGCTGCTG GTGGGCAGCC AGCAGGGTTT GGGGGTGTTC 
CAGTGTTGGT CGATAGAAGA AGGATTCCAG CAGCTTGTGG CCAGCTACTG CCCAGAGGTG 
ATGGAGGAGG GGGTAATGGA CCAAAAGAAC CAGCGTGATG GTACCCCAGT CATTATCAAC 
ACATCACGAG TGAGTGCACC GGCTGGTGGC AGGGCCAGCT GGGGTGCGGA CAAGTCCTAC 
TGGAATGAAT TCCTGGTGAT GTGTACTCTG TTTGTGTTTG CTATGGTGCT TTTGTTTCTG 
TTCTTTCTCT ACCGACATCG GGATGGCATG AAACTCTTCC TAAAGCAGGG CGAATGTGCC 
AGTGTGCACC CCAAGACTCG CCCTATAGTG CTACCACCTG AGACCCGACC GCTGAATGGT 
GTCGGCCCTC CTAGCACCCC ACTTGACCAC CGAGGCTACC AGGCTCTGTC GGATAGCTCC 
CCAGGGCCCA GAGTCTTCAC TGAATCAGAG AAGAGGCCAC TGAGCATCCA GGACAGCTTT 
GTAGAGGTGT CTCCCGTGTG TCCCCGGCCC CGAGTTCGAC TGGGCTCTGA GATCCGAGAC 
TCTGTGGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038687.2
CDS	286..2757
Translation	MGRASRSAVLRRALLLLLLLLLLRTTTTRALGPRISVPLGSEERLIRKFEAENISNYTALLLSQDGKTLYVGAREALFALNSNLSFLPGGEYQELLWSADADRKQQCSFKGKDPKRDCQNYIKILLPLNSSHLLTCGTAAFSPLCAYIHIASFTLAQDEAGNVILEDGKGRCPFDPNFKSTALVVDGELYTGTVSSFQGNDPAISRSQSSRPTKTESSLNWLQDPAFVASAYVPESLGSPIGDDDKIYFFFSETGQEFEFFENTIVSRVARVCKGDEGGERVLQQRWTSFLKAQLLCSRPDDGFPFNVLQDVFTLNPNPQDWRKTLFYGVFTSQWHRGTTEGSAICVFTMNDVQKAFDGLYKKVNRETQQWYTETHQVPTPRPGACITNSARERKINSSLQLPDRVLNFLKDHFLMDGQVRSRLLLLQPRARYQRVAVHRVPGLHSTYDVLFLGTGDGRLHKAVTLSSRVHIIEELQIFPQGQPVQNLLLDSHGGLLYASSHSGVVQVPVANCSLYPTCGDCLLARDPYCAWTGSACRLASLYQPDLASRPWTQDIEGASVKELCKNSSYKARFLVPGKPCKQVQIQPNTVNTLACPLLSNLATRLWVHNGAPVNASASCRVLPTGDLLLVGSQQGLGVFQCWSIEEGFQQLVASYCPEVMEEGVMDQKNQRDGTPVIINTSRVSAPAGGRASWGADKSYWNEFLVMCTLFVFAMVLLFLFFLYRHRDGMKLFLKQGECASVHPKTRPIVLPPETRPLNGVGPPSTPLDHRGYQALSDSSPGPRVFTESEKRPLSIQDSFVEVSPVCPRPRVRLGSEIRDSVV

Target ORF information:

RefSeq Version	NM_013659.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (Sema4b), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013659.4

ORF Insert Sequence:

ATGGGCCGGG CGAGCCGCTC CGCTGTCCTG CGGCGCGCGC TGCTGCTGCT GCTGCTACTG 
CTGCTGCTGC GGACGACGAC TACGCGGGCG CTCGGCCCCC GGATCAGTGT GCCGCTGGGC 
TCTGAAGAAA GGCTGATTAG AAAATTTGAA GCTGAAAACA TCTCCAACTA CACGGCCCTT 
CTGCTGAGCC AGGATGGCAA GACGCTGTAT GTGGGGGCCC GAGAGGCCCT CTTTGCACTT 
AACAGCAACC TCAGCTTCTT GCCAGGCGGG GAGTACCAAG AGCTGCTGTG GAGTGCAGAT 
GCTGACAGGA AGCAGCAGTG CAGCTTCAAG GGCAAGGACC CAAAGCGTGA CTGTCAAAAC 
TACATCAAGA TCCTCCTGCC ACTCAACAGC AGCCACCTGC TCACCTGTGG CACGGCCGCC 
TTCAGCCCCC TGTGTGCTTA CATTCACATA GCGAGCTTTA CTTTAGCCCA AGATGAGGCC 
GGCAATGTCA TTCTGGAGGA TGGCAAGGGT CGTTGTCCCT TTGACCCCAA CTTCAAGTCC 
ACGGCTCTGG TGGTTGATGG TGAGCTGTAC ACTGGAACAG TCAGTAGCTT CCAGGGAAAC 
GACCCAGCCA TTTCCCGGAG CCAGAGTTCC CGCCCCACCA AGACTGAGAG CTCCCTCAAC 
TGGCTACAAG ACCCTGCCTT TGTGGCCTCG GCCTACGTCC CCGAGAGCCT GGGCAGCCCC 
ATAGGTGATG ATGATAAGAT CTACTTCTTC TTCAGCGAGA CGGGCCAGGA GTTTGAGTTC 
TTTGAGAACA CCATCGTGTC CCGAGTTGCC CGAGTCTGTA AGGGCGATGA GGGTGGAGAG 
CGGGTGTTGC AGCAACGCTG GACCTCCTTT CTCAAGGCTC AGCTCCTGTG CTCCCGGCCT 
GATGATGGCT TTCCCTTTAA CGTGCTACAA GATGTCTTCA CCCTGAACCC CAACCCTCAG 
GATTGGCGCA AGACCCTTTT CTATGGGGTC TTTACCTCCC AGTGGCACAG AGGGACCACA 
GAAGGCTCTG CCATCTGCGT CTTCACCATG AATGATGTGC AGAAGGCCTT TGACGGCCTG 
TACAAGAAAG TAAACAGAGA GACACAGCAG TGGTATACCG AGACCCACCA GGTGCCCACA 
CCGCGGCCGG GAGCGTGCAT TACCAACAGT GCCCGGGAAC GGAAGATCAA CTCGTCCCTG 
CAGCTCCCAG ACCGAGTGCT GAACTTCCTC AAGGATCACT TCTTGATGGA TGGGCAGGTC 
CGCAGTCGCC TGCTGCTGCT GCAGCCCAGA GCCCGCTACC AGCGTGTGGC TGTGCACCGT 
GTGCCTGGCC TGCACAGCAC TTATGATGTC CTATTTCTGG GCACTGGTGA TGGCCGCCTG 
CACAAAGCAG TGACCCTGAG CTCCAGAGTC CACATCATTG AGGAGCTGCA GATCTTCCCT 
CAAGGACAGC CTGTGCAGAA CCTGCTCTTG GACAGCCATG GGGGACTGTT GTATGCCTCC 
TCCCATTCCG GGGTGGTGCA AGTGCCCGTA GCCAACTGCA GCCTGTACCC AACCTGTGGA 
GACTGCCTCC TGGCTCGAGA CCCCTACTGC GCCTGGACTG GCTCTGCCTG CAGGCTCGCT 
AGCCTCTACC AGCCTGATCT GGCCTCCAGG CCATGGACCC AGGACATTGA GGGTGCCAGT 
GTCAAGGAAC TCTGCAAGAA TTCCTCATAC AAGGCCCGGT TTCTTGTGCC AGGTAAGCCA 
TGTAAACAAG TCCAGATCCA ACCAAACACA GTGAACACCC TGGCCTGCCC ACTCCTCTCA 
AACCTGGCCA CTCGGCTCTG GGTGCACAAT GGAGCCCCAG TCAATGCCTC TGCCTCCTGC 
CGCGTGTTAC CCACCGGGGA CCTGCTGCTG GTGGGCAGCC AGCAGGGTTT GGGGGTGTTC 
CAGTGTTGGT CGATAGAAGA AGGATTCCAG CAGCTTGTGG CCAGCTACTG CCCAGAGGTG 
ATGGAGGAGG GGGTAATGGA CCAAAAGAAC CAGCGTGATG GTACCCCAGT CATTATCAAC 
ACATCACGAG TGAGTGCACC GGCTGGTGGC AGGGCCAGCT GGGGTGCGGA CAAGTCCTAC 
TGGAATGAAT TCCTGGTGAT GTGTACTCTG TTTGTGTTTG CTATGGTGCT TTTGTTTCTG 
TTCTTTCTCT ACCGACATCG GGATGGCATG AAACTCTTCC TAAAGCAGGG CGAATGTGCC 
AGTGTGCACC CCAAGACTCG CCCTATAGTG CTACCACCTG AGACCCGACC GCTGAATGGT 
GTCGGCCCTC CTAGCACCCC ACTTGACCAC CGAGGCTACC AGGCTCTGTC GGATAGCTCC 
CCAGGGCCCA GAGTCTTCAC TGAATCAGAG AAGAGGCCAC TGAGCATCCA GGACAGCTTT 
GTAGAGGTGT CTCCCGTGTG TCCCCGGCCC CGAGTTCGAC TGGGCTCTGA GATCCGAGAC 
TCTGTGGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema4b ( XM_011250830.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011249132.1 Mus musculus sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (Sema4b), transcript variant X1, mRNA.

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CloneID	OMu09748
Clone ID Related Accession (Same CDS sequence)	NM_001360135.1 , XM_011250830.2 , NM_013659.4 , NM_001360135.2 , NM_013659.5
Accession Version	XM_011250830.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	semaphorin-4B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011250830.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCGGG CGAGCCGCTC CGCTGTCCTG CGGCGCGCGC TGCTGCTGCT GCTGCTACTG 
CTGCTGCTGC GGACGACGAC TACGCGGGCG CTCGGCCCCC GGATCAGTGT GCCGCTGGGC 
TCTGAAGAAA GGCTGATTAG AAAATTTGAA GCTGAAAACA TCTCCAACTA CACGGCCCTT 
CTGCTGAGCC AGGATGGCAA GACGCTGTAT GTGGGGGCCC GAGAGGCCCT CTTTGCACTT 
AACAGCAACC TCAGCTTCTT GCCAGGCGGG GAGTACCAAG AGCTGCTGTG GAGTGCAGAT 
GCTGACAGGA AGCAGCAGTG CAGCTTCAAG GGCAAGGACC CAAAGCGTGA CTGTCAAAAC 
TACATCAAGA TCCTCCTGCC ACTCAACAGC AGCCACCTGC TCACCTGTGG CACGGCCGCC 
TTCAGCCCCC TGTGTGCTTA CATTCACATA GCGAGCTTTA CTTTAGCCCA AGATGAGGCC 
GGCAATGTCA TTCTGGAGGA TGGCAAGGGT CGTTGTCCCT TTGACCCCAA CTTCAAGTCC 
ACGGCTCTGG TGGTTGATGG TGAGCTGTAC ACTGGAACAG TCAGTAGCTT CCAGGGAAAC 
GACCCAGCCA TTTCCCGGAG CCAGAGTTCC CGCCCCACCA AGACTGAGAG CTCCCTCAAC 
TGGCTACAAG ACCCTGCCTT TGTGGCCTCG GCCTACGTCC CCGAGAGCCT GGGCAGCCCC 
ATAGGTGATG ATGATAAGAT CTACTTCTTC TTCAGCGAGA CGGGCCAGGA GTTTGAGTTC 
TTTGAGAACA CCATCGTGTC CCGAGTTGCC CGAGTCTGTA AGGGCGATGA GGGTGGAGAG 
CGGGTGTTGC AGCAACGCTG GACCTCCTTT CTCAAGGCTC AGCTCCTGTG CTCCCGGCCT 
GATGATGGCT TTCCCTTTAA CGTGCTACAA GATGTCTTCA CCCTGAACCC CAACCCTCAG 
GATTGGCGCA AGACCCTTTT CTATGGGGTC TTTACCTCCC AGTGGCACAG AGGGACCACA 
GAAGGCTCTG CCATCTGCGT CTTCACCATG AATGATGTGC AGAAGGCCTT TGACGGCCTG 
TACAAGAAAG TAAACAGAGA GACACAGCAG TGGTATACCG AGACCCACCA GGTGCCCACA 
CCGCGGCCGG GAGCGTGCAT TACCAACAGT GCCCGGGAAC GGAAGATCAA CTCGTCCCTG 
CAGCTCCCAG ACCGAGTGCT GAACTTCCTC AAGGATCACT TCTTGATGGA TGGGCAGGTC 
CGCAGTCGCC TGCTGCTGCT GCAGCCCAGA GCCCGCTACC AGCGTGTGGC TGTGCACCGT 
GTGCCTGGCC TGCACAGCAC TTATGATGTC CTATTTCTGG GCACTGGTGA TGGCCGCCTG 
CACAAAGCAG TGACCCTGAG CTCCAGAGTC CACATCATTG AGGAGCTGCA GATCTTCCCT 
CAAGGACAGC CTGTGCAGAA CCTGCTCTTG GACAGCCATG GGGGACTGTT GTATGCCTCC 
TCCCATTCCG GGGTGGTGCA AGTGCCCGTA GCCAACTGCA GCCTGTACCC AACCTGTGGA 
GACTGCCTCC TGGCTCGAGA CCCCTACTGC GCCTGGACTG GCTCTGCCTG CAGGCTCGCT 
AGCCTCTACC AGCCTGATCT GGCCTCCAGG CCATGGACCC AGGACATTGA GGGTGCCAGT 
GTCAAGGAAC TCTGCAAGAA TTCCTCATAC AAGGCCCGGT TTCTTGTGCC AGGTAAGCCA 
TGTAAACAAG TCCAGATCCA ACCAAACACA GTGAACACCC TGGCCTGCCC ACTCCTCTCA 
AACCTGGCCA CTCGGCTCTG GGTGCACAAT GGAGCCCCAG TCAATGCCTC TGCCTCCTGC 
CGCGTGTTAC CCACCGGGGA CCTGCTGCTG GTGGGCAGCC AGCAGGGTTT GGGGGTGTTC 
CAGTGTTGGT CGATAGAAGA AGGATTCCAG CAGCTTGTGG CCAGCTACTG CCCAGAGGTG 
ATGGAGGAGG GGGTAATGGA CCAAAAGAAC CAGCGTGATG GTACCCCAGT CATTATCAAC 
ACATCACGAG TGAGTGCACC GGCTGGTGGC AGGGCCAGCT GGGGTGCGGA CAAGTCCTAC 
TGGAATGAAT TCCTGGTGAT GTGTACTCTG TTTGTGTTTG CTATGGTGCT TTTGTTTCTG 
TTCTTTCTCT ACCGACATCG GGATGGCATG AAACTCTTCC TAAAGCAGGG CGAATGTGCC 
AGTGTGCACC CCAAGACTCG CCCTATAGTG CTACCACCTG AGACCCGACC GCTGAATGGT 
GTCGGCCCTC CTAGCACCCC ACTTGACCAC CGAGGCTACC AGGCTCTGTC GGATAGCTCC 
CCAGGGCCCA GAGTCTTCAC TGAATCAGAG AAGAGGCCAC TGAGCATCCA GGACAGCTTT 
GTAGAGGTGT CTCCCGTGTG TCCCCGGCCC CGAGTTCGAC TGGGCTCTGA GATCCGAGAC 
TCTGTGGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011249132.1
CDS	315..2786
Translation	MGRASRSAVLRRALLLLLLLLLLRTTTTRALGPRISVPLGSEERLIRKFEAENISNYTALLLSQDGKTLYVGAREALFALNSNLSFLPGGEYQELLWSADADRKQQCSFKGKDPKRDCQNYIKILLPLNSSHLLTCGTAAFSPLCAYIHIASFTLAQDEAGNVILEDGKGRCPFDPNFKSTALVVDGELYTGTVSSFQGNDPAISRSQSSRPTKTESSLNWLQDPAFVASAYVPESLGSPIGDDDKIYFFFSETGQEFEFFENTIVSRVARVCKGDEGGERVLQQRWTSFLKAQLLCSRPDDGFPFNVLQDVFTLNPNPQDWRKTLFYGVFTSQWHRGTTEGSAICVFTMNDVQKAFDGLYKKVNRETQQWYTETHQVPTPRPGACITNSARERKINSSLQLPDRVLNFLKDHFLMDGQVRSRLLLLQPRARYQRVAVHRVPGLHSTYDVLFLGTGDGRLHKAVTLSSRVHIIEELQIFPQGQPVQNLLLDSHGGLLYASSHSGVVQVPVANCSLYPTCGDCLLARDPYCAWTGSACRLASLYQPDLASRPWTQDIEGASVKELCKNSSYKARFLVPGKPCKQVQIQPNTVNTLACPLLSNLATRLWVHNGAPVNASASCRVLPTGDLLLVGSQQGLGVFQCWSIEEGFQQLVASYCPEVMEEGVMDQKNQRDGTPVIINTSRVSAPAGGRASWGADKSYWNEFLVMCTLFVFAMVLLFLFFLYRHRDGMKLFLKQGECASVHPKTRPIVLPPETRPLNGVGPPSTPLDHRGYQALSDSSPGPRVFTESEKRPLSIQDSFVEVSPVCPRPRVRLGSEIRDSVV

Target ORF information:

RefSeq Version	XM_011250830.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (Sema4b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250830.2

ORF Insert Sequence:

ATGGGCCGGG CGAGCCGCTC CGCTGTCCTG CGGCGCGCGC TGCTGCTGCT GCTGCTACTG 
CTGCTGCTGC GGACGACGAC TACGCGGGCG CTCGGCCCCC GGATCAGTGT GCCGCTGGGC 
TCTGAAGAAA GGCTGATTAG AAAATTTGAA GCTGAAAACA TCTCCAACTA CACGGCCCTT 
CTGCTGAGCC AGGATGGCAA GACGCTGTAT GTGGGGGCCC GAGAGGCCCT CTTTGCACTT 
AACAGCAACC TCAGCTTCTT GCCAGGCGGG GAGTACCAAG AGCTGCTGTG GAGTGCAGAT 
GCTGACAGGA AGCAGCAGTG CAGCTTCAAG GGCAAGGACC CAAAGCGTGA CTGTCAAAAC 
TACATCAAGA TCCTCCTGCC ACTCAACAGC AGCCACCTGC TCACCTGTGG CACGGCCGCC 
TTCAGCCCCC TGTGTGCTTA CATTCACATA GCGAGCTTTA CTTTAGCCCA AGATGAGGCC 
GGCAATGTCA TTCTGGAGGA TGGCAAGGGT CGTTGTCCCT TTGACCCCAA CTTCAAGTCC 
ACGGCTCTGG TGGTTGATGG TGAGCTGTAC ACTGGAACAG TCAGTAGCTT CCAGGGAAAC 
GACCCAGCCA TTTCCCGGAG CCAGAGTTCC CGCCCCACCA AGACTGAGAG CTCCCTCAAC 
TGGCTACAAG ACCCTGCCTT TGTGGCCTCG GCCTACGTCC CCGAGAGCCT GGGCAGCCCC 
ATAGGTGATG ATGATAAGAT CTACTTCTTC TTCAGCGAGA CGGGCCAGGA GTTTGAGTTC 
TTTGAGAACA CCATCGTGTC CCGAGTTGCC CGAGTCTGTA AGGGCGATGA GGGTGGAGAG 
CGGGTGTTGC AGCAACGCTG GACCTCCTTT CTCAAGGCTC AGCTCCTGTG CTCCCGGCCT 
GATGATGGCT TTCCCTTTAA CGTGCTACAA GATGTCTTCA CCCTGAACCC CAACCCTCAG 
GATTGGCGCA AGACCCTTTT CTATGGGGTC TTTACCTCCC AGTGGCACAG AGGGACCACA 
GAAGGCTCTG CCATCTGCGT CTTCACCATG AATGATGTGC AGAAGGCCTT TGACGGCCTG 
TACAAGAAAG TAAACAGAGA GACACAGCAG TGGTATACCG AGACCCACCA GGTGCCCACA 
CCGCGGCCGG GAGCGTGCAT TACCAACAGT GCCCGGGAAC GGAAGATCAA CTCGTCCCTG 
CAGCTCCCAG ACCGAGTGCT GAACTTCCTC AAGGATCACT TCTTGATGGA TGGGCAGGTC 
CGCAGTCGCC TGCTGCTGCT GCAGCCCAGA GCCCGCTACC AGCGTGTGGC TGTGCACCGT 
GTGCCTGGCC TGCACAGCAC TTATGATGTC CTATTTCTGG GCACTGGTGA TGGCCGCCTG 
CACAAAGCAG TGACCCTGAG CTCCAGAGTC CACATCATTG AGGAGCTGCA GATCTTCCCT 
CAAGGACAGC CTGTGCAGAA CCTGCTCTTG GACAGCCATG GGGGACTGTT GTATGCCTCC 
TCCCATTCCG GGGTGGTGCA AGTGCCCGTA GCCAACTGCA GCCTGTACCC AACCTGTGGA 
GACTGCCTCC TGGCTCGAGA CCCCTACTGC GCCTGGACTG GCTCTGCCTG CAGGCTCGCT 
AGCCTCTACC AGCCTGATCT GGCCTCCAGG CCATGGACCC AGGACATTGA GGGTGCCAGT 
GTCAAGGAAC TCTGCAAGAA TTCCTCATAC AAGGCCCGGT TTCTTGTGCC AGGTAAGCCA 
TGTAAACAAG TCCAGATCCA ACCAAACACA GTGAACACCC TGGCCTGCCC ACTCCTCTCA 
AACCTGGCCA CTCGGCTCTG GGTGCACAAT GGAGCCCCAG TCAATGCCTC TGCCTCCTGC 
CGCGTGTTAC CCACCGGGGA CCTGCTGCTG GTGGGCAGCC AGCAGGGTTT GGGGGTGTTC 
CAGTGTTGGT CGATAGAAGA AGGATTCCAG CAGCTTGTGG CCAGCTACTG CCCAGAGGTG 
ATGGAGGAGG GGGTAATGGA CCAAAAGAAC CAGCGTGATG GTACCCCAGT CATTATCAAC 
ACATCACGAG TGAGTGCACC GGCTGGTGGC AGGGCCAGCT GGGGTGCGGA CAAGTCCTAC 
TGGAATGAAT TCCTGGTGAT GTGTACTCTG TTTGTGTTTG CTATGGTGCT TTTGTTTCTG 
TTCTTTCTCT ACCGACATCG GGATGGCATG AAACTCTTCC TAAAGCAGGG CGAATGTGCC 
AGTGTGCACC CCAAGACTCG CCCTATAGTG CTACCACCTG AGACCCGACC GCTGAATGGT 
GTCGGCCCTC CTAGCACCCC ACTTGACCAC CGAGGCTACC AGGCTCTGTC GGATAGCTCC 
CCAGGGCCCA GAGTCTTCAC TGAATCAGAG AAGAGGCCAC TGAGCATCCA GGACAGCTTT 
GTAGAGGTGT CTCCCGTGTG TCCCCGGCCC CGAGTTCGAC TGGGCTCTGA GATCCGAGAC 
TCTGTGGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema4b ( NM_001360135.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001347064.1 Mus musculus sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (Sema4b), transcript variant 2, mRNA.

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CloneID	OMu09748
Clone ID Related Accession (Same CDS sequence)	NM_001360135.1 , XM_011250830.2 , NM_013659.4 , NM_001360135.2 , NM_013659.5
Accession Version	NM_001360135.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-04
Organism	Mus musculus(house mouse)
Product	semaphorin-4B precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC109232.17. On Dec 5, 2019 this sequence version replaced NM_001360135.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK154969.1, AK134973.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCCGGG CGAGCCGCTC CGCTGTCCTG CGGCGCGCGC TGCTGCTGCT GCTGCTACTG 
CTGCTGCTGC GGACGACGAC TACGCGGGCG CTCGGCCCCC GGATCAGTGT GCCGCTGGGC 
TCTGAAGAAA GGCTGATTAG AAAATTTGAA GCTGAAAACA TCTCCAACTA CACGGCCCTT 
CTGCTGAGCC AGGATGGCAA GACGCTGTAT GTGGGGGCCC GAGAGGCCCT CTTTGCACTT 
AACAGCAACC TCAGCTTCTT GCCAGGCGGG GAGTACCAAG AGCTGCTGTG GAGTGCAGAT 
GCTGACAGGA AGCAGCAGTG CAGCTTCAAG GGCAAGGACC CAAAGCGTGA CTGTCAAAAC 
TACATCAAGA TCCTCCTGCC ACTCAACAGC AGCCACCTGC TCACCTGTGG CACGGCCGCC 
TTCAGCCCCC TGTGTGCTTA CATTCACATA GCGAGCTTTA CTTTAGCCCA AGATGAGGCC 
GGCAATGTCA TTCTGGAGGA TGGCAAGGGT CGTTGTCCCT TTGACCCCAA CTTCAAGTCC 
ACGGCTCTGG TGGTTGATGG TGAGCTGTAC ACTGGAACAG TCAGTAGCTT CCAGGGAAAC 
GACCCAGCCA TTTCCCGGAG CCAGAGTTCC CGCCCCACCA AGACTGAGAG CTCCCTCAAC 
TGGCTACAAG ACCCTGCCTT TGTGGCCTCG GCCTACGTCC CCGAGAGCCT GGGCAGCCCC 
ATAGGTGATG ATGATAAGAT CTACTTCTTC TTCAGCGAGA CGGGCCAGGA GTTTGAGTTC 
TTTGAGAACA CCATCGTGTC CCGAGTTGCC CGAGTCTGTA AGGGCGATGA GGGTGGAGAG 
CGGGTGTTGC AGCAACGCTG GACCTCCTTT CTCAAGGCTC AGCTCCTGTG CTCCCGGCCT 
GATGATGGCT TTCCCTTTAA CGTGCTACAA GATGTCTTCA CCCTGAACCC CAACCCTCAG 
GATTGGCGCA AGACCCTTTT CTATGGGGTC TTTACCTCCC AGTGGCACAG AGGGACCACA 
GAAGGCTCTG CCATCTGCGT CTTCACCATG AATGATGTGC AGAAGGCCTT TGACGGCCTG 
TACAAGAAAG TAAACAGAGA GACACAGCAG TGGTATACCG AGACCCACCA GGTGCCCACA 
CCGCGGCCGG GAGCGTGCAT TACCAACAGT GCCCGGGAAC GGAAGATCAA CTCGTCCCTG 
CAGCTCCCAG ACCGAGTGCT GAACTTCCTC AAGGATCACT TCTTGATGGA TGGGCAGGTC 
CGCAGTCGCC TGCTGCTGCT GCAGCCCAGA GCCCGCTACC AGCGTGTGGC TGTGCACCGT 
GTGCCTGGCC TGCACAGCAC TTATGATGTC CTATTTCTGG GCACTGGTGA TGGCCGCCTG 
CACAAAGCAG TGACCCTGAG CTCCAGAGTC CACATCATTG AGGAGCTGCA GATCTTCCCT 
CAAGGACAGC CTGTGCAGAA CCTGCTCTTG GACAGCCATG GGGGACTGTT GTATGCCTCC 
TCCCATTCCG GGGTGGTGCA AGTGCCCGTA GCCAACTGCA GCCTGTACCC AACCTGTGGA 
GACTGCCTCC TGGCTCGAGA CCCCTACTGC GCCTGGACTG GCTCTGCCTG CAGGCTCGCT 
AGCCTCTACC AGCCTGATCT GGCCTCCAGG CCATGGACCC AGGACATTGA GGGTGCCAGT 
GTCAAGGAAC TCTGCAAGAA TTCCTCATAC AAGGCCCGGT TTCTTGTGCC AGGTAAGCCA 
TGTAAACAAG TCCAGATCCA ACCAAACACA GTGAACACCC TGGCCTGCCC ACTCCTCTCA 
AACCTGGCCA CTCGGCTCTG GGTGCACAAT GGAGCCCCAG TCAATGCCTC TGCCTCCTGC 
CGCGTGTTAC CCACCGGGGA CCTGCTGCTG GTGGGCAGCC AGCAGGGTTT GGGGGTGTTC 
CAGTGTTGGT CGATAGAAGA AGGATTCCAG CAGCTTGTGG CCAGCTACTG CCCAGAGGTG 
ATGGAGGAGG GGGTAATGGA CCAAAAGAAC CAGCGTGATG GTACCCCAGT CATTATCAAC 
ACATCACGAG TGAGTGCACC GGCTGGTGGC AGGGCCAGCT GGGGTGCGGA CAAGTCCTAC 
TGGAATGAAT TCCTGGTGAT GTGTACTCTG TTTGTGTTTG CTATGGTGCT TTTGTTTCTG 
TTCTTTCTCT ACCGACATCG GGATGGCATG AAACTCTTCC TAAAGCAGGG CGAATGTGCC 
AGTGTGCACC CCAAGACTCG CCCTATAGTG CTACCACCTG AGACCCGACC GCTGAATGGT 
GTCGGCCCTC CTAGCACCCC ACTTGACCAC CGAGGCTACC AGGCTCTGTC GGATAGCTCC 
CCAGGGCCCA GAGTCTTCAC TGAATCAGAG AAGAGGCCAC TGAGCATCCA GGACAGCTTT 
GTAGAGGTGT CTCCCGTGTG TCCCCGGCCC CGAGTTCGAC TGGGCTCTGA GATCCGAGAC 
TCTGTGGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001347064.1
CDS	66..2537
Translation	MGRASRSAVLRRALLLLLLLLLLRTTTTRALGPRISVPLGSEERLIRKFEAENISNYTALLLSQDGKTLYVGAREALFALNSNLSFLPGGEYQELLWSADADRKQQCSFKGKDPKRDCQNYIKILLPLNSSHLLTCGTAAFSPLCAYIHIASFTLAQDEAGNVILEDGKGRCPFDPNFKSTALVVDGELYTGTVSSFQGNDPAISRSQSSRPTKTESSLNWLQDPAFVASAYVPESLGSPIGDDDKIYFFFSETGQEFEFFENTIVSRVARVCKGDEGGERVLQQRWTSFLKAQLLCSRPDDGFPFNVLQDVFTLNPNPQDWRKTLFYGVFTSQWHRGTTEGSAICVFTMNDVQKAFDGLYKKVNRETQQWYTETHQVPTPRPGACITNSARERKINSSLQLPDRVLNFLKDHFLMDGQVRSRLLLLQPRARYQRVAVHRVPGLHSTYDVLFLGTGDGRLHKAVTLSSRVHIIEELQIFPQGQPVQNLLLDSHGGLLYASSHSGVVQVPVANCSLYPTCGDCLLARDPYCAWTGSACRLASLYQPDLASRPWTQDIEGASVKELCKNSSYKARFLVPGKPCKQVQIQPNTVNTLACPLLSNLATRLWVHNGAPVNASASCRVLPTGDLLLVGSQQGLGVFQCWSIEEGFQQLVASYCPEVMEEGVMDQKNQRDGTPVIINTSRVSAPAGGRASWGADKSYWNEFLVMCTLFVFAMVLLFLFFLYRHRDGMKLFLKQGECASVHPKTRPIVLPPETRPLNGVGPPSTPLDHRGYQALSDSSPGPRVFTESEKRPLSIQDSFVEVSPVCPRPRVRLGSEIRDSVV

Target ORF information:

RefSeq Version	NM_001360135.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (Sema4b), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001360135.2

ORF Insert Sequence:

ATGGGCCGGG CGAGCCGCTC CGCTGTCCTG CGGCGCGCGC TGCTGCTGCT GCTGCTACTG 
CTGCTGCTGC GGACGACGAC TACGCGGGCG CTCGGCCCCC GGATCAGTGT GCCGCTGGGC 
TCTGAAGAAA GGCTGATTAG AAAATTTGAA GCTGAAAACA TCTCCAACTA CACGGCCCTT 
CTGCTGAGCC AGGATGGCAA GACGCTGTAT GTGGGGGCCC GAGAGGCCCT CTTTGCACTT 
AACAGCAACC TCAGCTTCTT GCCAGGCGGG GAGTACCAAG AGCTGCTGTG GAGTGCAGAT 
GCTGACAGGA AGCAGCAGTG CAGCTTCAAG GGCAAGGACC CAAAGCGTGA CTGTCAAAAC 
TACATCAAGA TCCTCCTGCC ACTCAACAGC AGCCACCTGC TCACCTGTGG CACGGCCGCC 
TTCAGCCCCC TGTGTGCTTA CATTCACATA GCGAGCTTTA CTTTAGCCCA AGATGAGGCC 
GGCAATGTCA TTCTGGAGGA TGGCAAGGGT CGTTGTCCCT TTGACCCCAA CTTCAAGTCC 
ACGGCTCTGG TGGTTGATGG TGAGCTGTAC ACTGGAACAG TCAGTAGCTT CCAGGGAAAC 
GACCCAGCCA TTTCCCGGAG CCAGAGTTCC CGCCCCACCA AGACTGAGAG CTCCCTCAAC 
TGGCTACAAG ACCCTGCCTT TGTGGCCTCG GCCTACGTCC CCGAGAGCCT GGGCAGCCCC 
ATAGGTGATG ATGATAAGAT CTACTTCTTC TTCAGCGAGA CGGGCCAGGA GTTTGAGTTC 
TTTGAGAACA CCATCGTGTC CCGAGTTGCC CGAGTCTGTA AGGGCGATGA GGGTGGAGAG 
CGGGTGTTGC AGCAACGCTG GACCTCCTTT CTCAAGGCTC AGCTCCTGTG CTCCCGGCCT 
GATGATGGCT TTCCCTTTAA CGTGCTACAA GATGTCTTCA CCCTGAACCC CAACCCTCAG 
GATTGGCGCA AGACCCTTTT CTATGGGGTC TTTACCTCCC AGTGGCACAG AGGGACCACA 
GAAGGCTCTG CCATCTGCGT CTTCACCATG AATGATGTGC AGAAGGCCTT TGACGGCCTG 
TACAAGAAAG TAAACAGAGA GACACAGCAG TGGTATACCG AGACCCACCA GGTGCCCACA 
CCGCGGCCGG GAGCGTGCAT TACCAACAGT GCCCGGGAAC GGAAGATCAA CTCGTCCCTG 
CAGCTCCCAG ACCGAGTGCT GAACTTCCTC AAGGATCACT TCTTGATGGA TGGGCAGGTC 
CGCAGTCGCC TGCTGCTGCT GCAGCCCAGA GCCCGCTACC AGCGTGTGGC TGTGCACCGT 
GTGCCTGGCC TGCACAGCAC TTATGATGTC CTATTTCTGG GCACTGGTGA TGGCCGCCTG 
CACAAAGCAG TGACCCTGAG CTCCAGAGTC CACATCATTG AGGAGCTGCA GATCTTCCCT 
CAAGGACAGC CTGTGCAGAA CCTGCTCTTG GACAGCCATG GGGGACTGTT GTATGCCTCC 
TCCCATTCCG GGGTGGTGCA AGTGCCCGTA GCCAACTGCA GCCTGTACCC AACCTGTGGA 
GACTGCCTCC TGGCTCGAGA CCCCTACTGC GCCTGGACTG GCTCTGCCTG CAGGCTCGCT 
AGCCTCTACC AGCCTGATCT GGCCTCCAGG CCATGGACCC AGGACATTGA GGGTGCCAGT 
GTCAAGGAAC TCTGCAAGAA TTCCTCATAC AAGGCCCGGT TTCTTGTGCC AGGTAAGCCA 
TGTAAACAAG TCCAGATCCA ACCAAACACA GTGAACACCC TGGCCTGCCC ACTCCTCTCA 
AACCTGGCCA CTCGGCTCTG GGTGCACAAT GGAGCCCCAG TCAATGCCTC TGCCTCCTGC 
CGCGTGTTAC CCACCGGGGA CCTGCTGCTG GTGGGCAGCC AGCAGGGTTT GGGGGTGTTC 
CAGTGTTGGT CGATAGAAGA AGGATTCCAG CAGCTTGTGG CCAGCTACTG CCCAGAGGTG 
ATGGAGGAGG GGGTAATGGA CCAAAAGAAC CAGCGTGATG GTACCCCAGT CATTATCAAC 
ACATCACGAG TGAGTGCACC GGCTGGTGGC AGGGCCAGCT GGGGTGCGGA CAAGTCCTAC 
TGGAATGAAT TCCTGGTGAT GTGTACTCTG TTTGTGTTTG CTATGGTGCT TTTGTTTCTG 
TTCTTTCTCT ACCGACATCG GGATGGCATG AAACTCTTCC TAAAGCAGGG CGAATGTGCC 
AGTGTGCACC CCAAGACTCG CCCTATAGTG CTACCACCTG AGACCCGACC GCTGAATGGT 
GTCGGCCCTC CTAGCACCCC ACTTGACCAC CGAGGCTACC AGGCTCTGTC GGATAGCTCC 
CCAGGGCCCA GAGTCTTCAC TGAATCAGAG AAGAGGCCAC TGAGCATCCA GGACAGCTTT 
GTAGAGGTGT CTCCCGTGTG TCCCCGGCCC CGAGTTCGAC TGGGCTCTGA GATCCGAGAC 
TCTGTGGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema4b ( NM_013659.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038687.2 Mus musculus sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (Sema4b), transcript variant 1, mRNA.

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CloneID	OMu09748
Clone ID Related Accession (Same CDS sequence)	NM_001360135.1 , XM_011250830.2 , NM_013659.4 , NM_001360135.2 , NM_013659.5
Accession Version	NM_013659.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-04
Organism	Mus musculus(house mouse)
Product	semaphorin-4B precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC109232.17. On Dec 5, 2019 this sequence version replaced NM_013659.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK173249.1, AK145570.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCCGGG CGAGCCGCTC CGCTGTCCTG CGGCGCGCGC TGCTGCTGCT GCTGCTACTG 
CTGCTGCTGC GGACGACGAC TACGCGGGCG CTCGGCCCCC GGATCAGTGT GCCGCTGGGC 
TCTGAAGAAA GGCTGATTAG AAAATTTGAA GCTGAAAACA TCTCCAACTA CACGGCCCTT 
CTGCTGAGCC AGGATGGCAA GACGCTGTAT GTGGGGGCCC GAGAGGCCCT CTTTGCACTT 
AACAGCAACC TCAGCTTCTT GCCAGGCGGG GAGTACCAAG AGCTGCTGTG GAGTGCAGAT 
GCTGACAGGA AGCAGCAGTG CAGCTTCAAG GGCAAGGACC CAAAGCGTGA CTGTCAAAAC 
TACATCAAGA TCCTCCTGCC ACTCAACAGC AGCCACCTGC TCACCTGTGG CACGGCCGCC 
TTCAGCCCCC TGTGTGCTTA CATTCACATA GCGAGCTTTA CTTTAGCCCA AGATGAGGCC 
GGCAATGTCA TTCTGGAGGA TGGCAAGGGT CGTTGTCCCT TTGACCCCAA CTTCAAGTCC 
ACGGCTCTGG TGGTTGATGG TGAGCTGTAC ACTGGAACAG TCAGTAGCTT CCAGGGAAAC 
GACCCAGCCA TTTCCCGGAG CCAGAGTTCC CGCCCCACCA AGACTGAGAG CTCCCTCAAC 
TGGCTACAAG ACCCTGCCTT TGTGGCCTCG GCCTACGTCC CCGAGAGCCT GGGCAGCCCC 
ATAGGTGATG ATGATAAGAT CTACTTCTTC TTCAGCGAGA CGGGCCAGGA GTTTGAGTTC 
TTTGAGAACA CCATCGTGTC CCGAGTTGCC CGAGTCTGTA AGGGCGATGA GGGTGGAGAG 
CGGGTGTTGC AGCAACGCTG GACCTCCTTT CTCAAGGCTC AGCTCCTGTG CTCCCGGCCT 
GATGATGGCT TTCCCTTTAA CGTGCTACAA GATGTCTTCA CCCTGAACCC CAACCCTCAG 
GATTGGCGCA AGACCCTTTT CTATGGGGTC TTTACCTCCC AGTGGCACAG AGGGACCACA 
GAAGGCTCTG CCATCTGCGT CTTCACCATG AATGATGTGC AGAAGGCCTT TGACGGCCTG 
TACAAGAAAG TAAACAGAGA GACACAGCAG TGGTATACCG AGACCCACCA GGTGCCCACA 
CCGCGGCCGG GAGCGTGCAT TACCAACAGT GCCCGGGAAC GGAAGATCAA CTCGTCCCTG 
CAGCTCCCAG ACCGAGTGCT GAACTTCCTC AAGGATCACT TCTTGATGGA TGGGCAGGTC 
CGCAGTCGCC TGCTGCTGCT GCAGCCCAGA GCCCGCTACC AGCGTGTGGC TGTGCACCGT 
GTGCCTGGCC TGCACAGCAC TTATGATGTC CTATTTCTGG GCACTGGTGA TGGCCGCCTG 
CACAAAGCAG TGACCCTGAG CTCCAGAGTC CACATCATTG AGGAGCTGCA GATCTTCCCT 
CAAGGACAGC CTGTGCAGAA CCTGCTCTTG GACAGCCATG GGGGACTGTT GTATGCCTCC 
TCCCATTCCG GGGTGGTGCA AGTGCCCGTA GCCAACTGCA GCCTGTACCC AACCTGTGGA 
GACTGCCTCC TGGCTCGAGA CCCCTACTGC GCCTGGACTG GCTCTGCCTG CAGGCTCGCT 
AGCCTCTACC AGCCTGATCT GGCCTCCAGG CCATGGACCC AGGACATTGA GGGTGCCAGT 
GTCAAGGAAC TCTGCAAGAA TTCCTCATAC AAGGCCCGGT TTCTTGTGCC AGGTAAGCCA 
TGTAAACAAG TCCAGATCCA ACCAAACACA GTGAACACCC TGGCCTGCCC ACTCCTCTCA 
AACCTGGCCA CTCGGCTCTG GGTGCACAAT GGAGCCCCAG TCAATGCCTC TGCCTCCTGC 
CGCGTGTTAC CCACCGGGGA CCTGCTGCTG GTGGGCAGCC AGCAGGGTTT GGGGGTGTTC 
CAGTGTTGGT CGATAGAAGA AGGATTCCAG CAGCTTGTGG CCAGCTACTG CCCAGAGGTG 
ATGGAGGAGG GGGTAATGGA CCAAAAGAAC CAGCGTGATG GTACCCCAGT CATTATCAAC 
ACATCACGAG TGAGTGCACC GGCTGGTGGC AGGGCCAGCT GGGGTGCGGA CAAGTCCTAC 
TGGAATGAAT TCCTGGTGAT GTGTACTCTG TTTGTGTTTG CTATGGTGCT TTTGTTTCTG 
TTCTTTCTCT ACCGACATCG GGATGGCATG AAACTCTTCC TAAAGCAGGG CGAATGTGCC 
AGTGTGCACC CCAAGACTCG CCCTATAGTG CTACCACCTG AGACCCGACC GCTGAATGGT 
GTCGGCCCTC CTAGCACCCC ACTTGACCAC CGAGGCTACC AGGCTCTGTC GGATAGCTCC 
CCAGGGCCCA GAGTCTTCAC TGAATCAGAG AAGAGGCCAC TGAGCATCCA GGACAGCTTT 
GTAGAGGTGT CTCCCGTGTG TCCCCGGCCC CGAGTTCGAC TGGGCTCTGA GATCCGAGAC 
TCTGTGGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038687.2
CDS	257..2728
Translation	MGRASRSAVLRRALLLLLLLLLLRTTTTRALGPRISVPLGSEERLIRKFEAENISNYTALLLSQDGKTLYVGAREALFALNSNLSFLPGGEYQELLWSADADRKQQCSFKGKDPKRDCQNYIKILLPLNSSHLLTCGTAAFSPLCAYIHIASFTLAQDEAGNVILEDGKGRCPFDPNFKSTALVVDGELYTGTVSSFQGNDPAISRSQSSRPTKTESSLNWLQDPAFVASAYVPESLGSPIGDDDKIYFFFSETGQEFEFFENTIVSRVARVCKGDEGGERVLQQRWTSFLKAQLLCSRPDDGFPFNVLQDVFTLNPNPQDWRKTLFYGVFTSQWHRGTTEGSAICVFTMNDVQKAFDGLYKKVNRETQQWYTETHQVPTPRPGACITNSARERKINSSLQLPDRVLNFLKDHFLMDGQVRSRLLLLQPRARYQRVAVHRVPGLHSTYDVLFLGTGDGRLHKAVTLSSRVHIIEELQIFPQGQPVQNLLLDSHGGLLYASSHSGVVQVPVANCSLYPTCGDCLLARDPYCAWTGSACRLASLYQPDLASRPWTQDIEGASVKELCKNSSYKARFLVPGKPCKQVQIQPNTVNTLACPLLSNLATRLWVHNGAPVNASASCRVLPTGDLLLVGSQQGLGVFQCWSIEEGFQQLVASYCPEVMEEGVMDQKNQRDGTPVIINTSRVSAPAGGRASWGADKSYWNEFLVMCTLFVFAMVLLFLFFLYRHRDGMKLFLKQGECASVHPKTRPIVLPPETRPLNGVGPPSTPLDHRGYQALSDSSPGPRVFTESEKRPLSIQDSFVEVSPVCPRPRVRLGSEIRDSVV

Target ORF information:

RefSeq Version	NM_013659.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (Sema4b), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013659.5

ORF Insert Sequence:

ATGGGCCGGG CGAGCCGCTC CGCTGTCCTG CGGCGCGCGC TGCTGCTGCT GCTGCTACTG 
CTGCTGCTGC GGACGACGAC TACGCGGGCG CTCGGCCCCC GGATCAGTGT GCCGCTGGGC 
TCTGAAGAAA GGCTGATTAG AAAATTTGAA GCTGAAAACA TCTCCAACTA CACGGCCCTT 
CTGCTGAGCC AGGATGGCAA GACGCTGTAT GTGGGGGCCC GAGAGGCCCT CTTTGCACTT 
AACAGCAACC TCAGCTTCTT GCCAGGCGGG GAGTACCAAG AGCTGCTGTG GAGTGCAGAT 
GCTGACAGGA AGCAGCAGTG CAGCTTCAAG GGCAAGGACC CAAAGCGTGA CTGTCAAAAC 
TACATCAAGA TCCTCCTGCC ACTCAACAGC AGCCACCTGC TCACCTGTGG CACGGCCGCC 
TTCAGCCCCC TGTGTGCTTA CATTCACATA GCGAGCTTTA CTTTAGCCCA AGATGAGGCC 
GGCAATGTCA TTCTGGAGGA TGGCAAGGGT CGTTGTCCCT TTGACCCCAA CTTCAAGTCC 
ACGGCTCTGG TGGTTGATGG TGAGCTGTAC ACTGGAACAG TCAGTAGCTT CCAGGGAAAC 
GACCCAGCCA TTTCCCGGAG CCAGAGTTCC CGCCCCACCA AGACTGAGAG CTCCCTCAAC 
TGGCTACAAG ACCCTGCCTT TGTGGCCTCG GCCTACGTCC CCGAGAGCCT GGGCAGCCCC 
ATAGGTGATG ATGATAAGAT CTACTTCTTC TTCAGCGAGA CGGGCCAGGA GTTTGAGTTC 
TTTGAGAACA CCATCGTGTC CCGAGTTGCC CGAGTCTGTA AGGGCGATGA GGGTGGAGAG 
CGGGTGTTGC AGCAACGCTG GACCTCCTTT CTCAAGGCTC AGCTCCTGTG CTCCCGGCCT 
GATGATGGCT TTCCCTTTAA CGTGCTACAA GATGTCTTCA CCCTGAACCC CAACCCTCAG 
GATTGGCGCA AGACCCTTTT CTATGGGGTC TTTACCTCCC AGTGGCACAG AGGGACCACA 
GAAGGCTCTG CCATCTGCGT CTTCACCATG AATGATGTGC AGAAGGCCTT TGACGGCCTG 
TACAAGAAAG TAAACAGAGA GACACAGCAG TGGTATACCG AGACCCACCA GGTGCCCACA 
CCGCGGCCGG GAGCGTGCAT TACCAACAGT GCCCGGGAAC GGAAGATCAA CTCGTCCCTG 
CAGCTCCCAG ACCGAGTGCT GAACTTCCTC AAGGATCACT TCTTGATGGA TGGGCAGGTC 
CGCAGTCGCC TGCTGCTGCT GCAGCCCAGA GCCCGCTACC AGCGTGTGGC TGTGCACCGT 
GTGCCTGGCC TGCACAGCAC TTATGATGTC CTATTTCTGG GCACTGGTGA TGGCCGCCTG 
CACAAAGCAG TGACCCTGAG CTCCAGAGTC CACATCATTG AGGAGCTGCA GATCTTCCCT 
CAAGGACAGC CTGTGCAGAA CCTGCTCTTG GACAGCCATG GGGGACTGTT GTATGCCTCC 
TCCCATTCCG GGGTGGTGCA AGTGCCCGTA GCCAACTGCA GCCTGTACCC AACCTGTGGA 
GACTGCCTCC TGGCTCGAGA CCCCTACTGC GCCTGGACTG GCTCTGCCTG CAGGCTCGCT 
AGCCTCTACC AGCCTGATCT GGCCTCCAGG CCATGGACCC AGGACATTGA GGGTGCCAGT 
GTCAAGGAAC TCTGCAAGAA TTCCTCATAC AAGGCCCGGT TTCTTGTGCC AGGTAAGCCA 
TGTAAACAAG TCCAGATCCA ACCAAACACA GTGAACACCC TGGCCTGCCC ACTCCTCTCA 
AACCTGGCCA CTCGGCTCTG GGTGCACAAT GGAGCCCCAG TCAATGCCTC TGCCTCCTGC 
CGCGTGTTAC CCACCGGGGA CCTGCTGCTG GTGGGCAGCC AGCAGGGTTT GGGGGTGTTC 
CAGTGTTGGT CGATAGAAGA AGGATTCCAG CAGCTTGTGG CCAGCTACTG CCCAGAGGTG 
ATGGAGGAGG GGGTAATGGA CCAAAAGAAC CAGCGTGATG GTACCCCAGT CATTATCAAC 
ACATCACGAG TGAGTGCACC GGCTGGTGGC AGGGCCAGCT GGGGTGCGGA CAAGTCCTAC 
TGGAATGAAT TCCTGGTGAT GTGTACTCTG TTTGTGTTTG CTATGGTGCT TTTGTTTCTG 
TTCTTTCTCT ACCGACATCG GGATGGCATG AAACTCTTCC TAAAGCAGGG CGAATGTGCC 
AGTGTGCACC CCAAGACTCG CCCTATAGTG CTACCACCTG AGACCCGACC GCTGAATGGT 
GTCGGCCCTC CTAGCACCCC ACTTGACCAC CGAGGCTACC AGGCTCTGTC GGATAGCTCC 
CCAGGGCCCA GAGTCTTCAC TGAATCAGAG AAGAGGCCAC TGAGCATCCA GGACAGCTTT 
GTAGAGGTGT CTCCCGTGTG TCCCCGGCCC CGAGTTCGAC TGGGCTCTGA GATCCGAGAC 
TCTGTGGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema4b ( XM_030242259.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030098119.1 Mus musculus sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (Sema4b), transcript variant X1, mRNA.

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CloneID	OMu89399
Clone ID Related Accession (Same CDS sequence)	XM_030242259.1
Accession Version	XM_030242259.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2691bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-4B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATAGAC CTCTCGCGGA AGTAAGACTG GAAAGCTACC AGTCTCCGTC TCAGAGTCTG 
CACAGCTCCC AGAGCGGGGG TGGGGGTCCA GAGGCGGAGG CCGAGGGAAG GACTCCGGGC 
CGAACCGCGG GGCGGGGCTC CAGCCTGCGA TTCCACTTGA GCAACCTGAG CCGGAGGAGG 
ACGCCGCTGC CTGCTGCGGT CCGAGCTCCA CGCGCCGCGA TGGGCCGGGC GAGCCGCTCC 
GCTGTCCTGC GGCGCGCGCT GCTGCTGCTG CTGCTACTGC TGCTGCTGCG GACGACGACT 
ACGCGGGCGC TCGGCCCCCG GATCAGTGTG CCGCTGGGCT CTGAAGAAAG GCTGATTAGA 
AAATTTGAAG CTGAAAACAT CTCCAACTAC ACGGCCCTTC TGCTGAGCCA GGATGGCAAG 
ACGCTGTATG TGGGGGCCCG AGAGGCCCTC TTTGCACTTA ACAGCAACCT CAGCTTCTTG 
CCAGGCGGGG AGTACCAAGA GCTGCTGTGG AGTGCAGATG CTGACAGGAA GCAGCAGTGC 
AGCTTCAAGG GCAAGGACCC AAAGCGTGAC TGTCAAAACT ACATCAAGAT CCTCCTGCCA 
CTCAACAGCA GCCACCTGCT CACCTGTGGC ACGGCCGCCT TCAGCCCCCT GTGTGCTTAC 
ATTCACATAG CGAGCTTTAC TTTAGCCCAA GATGAGGCCG GCAATGTCAT TCTGGAGGAT 
GGCAAGGGTC GTTGTCCCTT TGACCCCAAC TTCAAGTCCA CGGCTCTGGT GGTTGATGGT 
GAGCTGTACA CTGGAACAGT CAGTAGCTTC CAGGGAAACG ACCCAGCCAT TTCCCGGAGC 
CAGAGTTCCC GCCCCACCAA GACTGAGAGC TCCCTCAACT GGCTACAAGA CCCTGCCTTT 
GTGGCCTCGG CCTACGTCCC CGAGAGCCTG GGCAGCCCCA TAGGTGATGA TGATAAGATC 
TACTTCTTCT TCAGCGAGAC GGGCCAGGAG TTTGAGTTCT TTGAGAACAC CATCGTGTCC 
CGAGTTGCCC GAGTCTGTAA GGGCGATGAG GGTGGAGAGC GGGTGTTGCA GCAACGCTGG 
ACCTCCTTTC TCAAGGCTCA GCTCCTGTGC TCCCGGCCTG ATGATGGCTT TCCCTTTAAC 
GTGCTACAAG ATGTCTTCAC CCTGAACCCC AACCCTCAGG ATTGGCGCAA GACCCTTTTC 
TATGGGGTCT TTACCTCCCA GTGGCACAGA GGGACCACAG AAGGCTCTGC CATCTGCGTC 
TTCACCATGA ATGATGTGCA GAAGGCCTTT GACGGCCTGT ACAAGAAAGT AAACAGAGAG 
ACACAGCAGT GGTATACCGA GACCCACCAG GTGCCCACAC CGCGGCCGGG AGCGTGCATT 
ACCAACAGTG CCCGGGAACG GAAGATCAAC TCGTCCCTGC AGCTCCCAGA CCGAGTGCTG 
AACTTCCTCA AGGATCACTT CTTGATGGAT GGGCAGGTCC GCAGTCGCCT GCTGCTGCTG 
CAGCCCAGAG CCCGCTACCA GCGTGTGGCT GTGCACCGTG TGCCTGGCCT GCACAGCACT 
TATGATGTCC TATTTCTGGG CACTGGTGAT GGCCGCCTGC ACAAAGCAGT GACCCTGAGC 
TCCAGAGTCC ACATCATTGA GGAGCTGCAG ATCTTCCCTC AAGGACAGCC TGTGCAGAAC 
CTGCTCTTGG ACAGCCATGG GGGACTGTTG TATGCCTCCT CCCATTCCGG GGTGGTGCAA 
GTGCCCGTAG CCAACTGCAG CCTGTACCCA ACCTGTGGAG ACTGCCTCCT GGCTCGAGAC 
CCCTACTGCG CCTGGACTGG CTCTGCCTGC AGGCTCGCTA GCCTCTACCA GCCTGATCTG 
GCCTCCAGGC CATGGACCCA GGACATTGAG GGTGCCAGTG TCAAGGAACT CTGCAAGAAT 
TCCTCATACA AGGCCCGGTT TCTTGTGCCA GGTAAGCCAT GTAAACAAGT CCAGATCCAA 
CCAAACACAG TGAACACCCT GGCCTGCCCA CTCCTCTCAA ACCTGGCCAC TCGGCTCTGG 
GTGCACAATG GAGCCCCAGT CAATGCCTCT GCCTCCTGCC GCGTGTTACC CACCGGGGAC 
CTGCTGCTGG TGGGCAGCCA GCAGGGTTTG GGGGTGTTCC AGTGTTGGTC GATAGAAGAA 
GGATTCCAGC AGCTTGTGGC CAGCTACTGC CCAGAGGTGA TGGAGGAGGG GGTAATGGAC 
CAAAAGAACC AGCGTGATGG TACCCCAGTC ATTATCAACA CATCACGAGT GAGTGCACCG 
GCTGGTGGCA GGGCCAGCTG GGGTGCGGAC AAGTCCTACT GGAATGAATT CCTGGTGATG 
TGTACTCTGT TTGTGTTTGC TATGGTGCTT TTGTTTCTGT TCTTTCTCTA CCGACATCGG 
GATGGCATGA AACTCTTCCT AAAGCAGGGC GAATGTGCCA GTGTGCACCC CAAGACTCGC 
CCTATAGTGC TACCACCTGA GACCCGACCG CTGAATGGTG TCGGCCCTCC TAGCACCCCA 
CTTGACCACC GAGGCTACCA GGCTCTGTCG GATAGCTCCC CAGGGCCCAG AGTCTTCACT 
GAATCAGAGA AGAGGCCACT GAGCATCCAG GACAGCTTTG TAGAGGTGTC TCCCGTGTGT 
CCCCGGCCCC GAGTTCGACT GGGCTCTGAG ATCCGAGACT CTGTGGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030098119.1
CDS	31..2721
Translation	MHRPLAEVRLESYQSPSQSLHSSQSGGGGPEAEAEGRTPGRTAGRGSSLRFHLSNLSRRRTPLPAAVRAPRAAMGRASRSAVLRRALLLLLLLLLLRTTTTRALGPRISVPLGSEERLIRKFEAENISNYTALLLSQDGKTLYVGAREALFALNSNLSFLPGGEYQELLWSADADRKQQCSFKGKDPKRDCQNYIKILLPLNSSHLLTCGTAAFSPLCAYIHIASFTLAQDEAGNVILEDGKGRCPFDPNFKSTALVVDGELYTGTVSSFQGNDPAISRSQSSRPTKTESSLNWLQDPAFVASAYVPESLGSPIGDDDKIYFFFSETGQEFEFFENTIVSRVARVCKGDEGGERVLQQRWTSFLKAQLLCSRPDDGFPFNVLQDVFTLNPNPQDWRKTLFYGVFTSQWHRGTTEGSAICVFTMNDVQKAFDGLYKKVNRETQQWYTETHQVPTPRPGACITNSARERKINSSLQLPDRVLNFLKDHFLMDGQVRSRLLLLQPRARYQRVAVHRVPGLHSTYDVLFLGTGDGRLHKAVTLSSRVHIIEELQIFPQGQPVQNLLLDSHGGLLYASSHSGVVQVPVANCSLYPTCGDCLLARDPYCAWTGSACRLASLYQPDLASRPWTQDIEGASVKELCKNSSYKARFLVPGKPCKQVQIQPNTVNTLACPLLSNLATRLWVHNGAPVNASASCRVLPTGDLLLVGSQQGLGVFQCWSIEEGFQQLVASYCPEVMEEGVMDQKNQRDGTPVIINTSRVSAPAGGRASWGADKSYWNEFLVMCTLFVFAMVLLFLFFLYRHRDGMKLFLKQGECASVHPKTRPIVLPPETRPLNGVGPPSTPLDHRGYQALSDSSPGPRVFTESEKRPLSIQDSFVEVSPVCPRPRVRLGSEIRDSVV

Target ORF information:

RefSeq Version	XM_030242259.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (Sema4b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030242259.1

ORF Insert Sequence:

ATGCATAGAC CTCTCGCGGA AGTAAGACTG GAAAGCTACC AGTCTCCGTC TCAGAGTCTG 
CACAGCTCCC AGAGCGGGGG TGGGGGTCCA GAGGCGGAGG CCGAGGGAAG GACTCCGGGC 
CGAACCGCGG GGCGGGGCTC CAGCCTGCGA TTCCACTTGA GCAACCTGAG CCGGAGGAGG 
ACGCCGCTGC CTGCTGCGGT CCGAGCTCCA CGCGCCGCGA TGGGCCGGGC GAGCCGCTCC 
GCTGTCCTGC GGCGCGCGCT GCTGCTGCTG CTGCTACTGC TGCTGCTGCG GACGACGACT 
ACGCGGGCGC TCGGCCCCCG GATCAGTGTG CCGCTGGGCT CTGAAGAAAG GCTGATTAGA 
AAATTTGAAG CTGAAAACAT CTCCAACTAC ACGGCCCTTC TGCTGAGCCA GGATGGCAAG 
ACGCTGTATG TGGGGGCCCG AGAGGCCCTC TTTGCACTTA ACAGCAACCT CAGCTTCTTG 
CCAGGCGGGG AGTACCAAGA GCTGCTGTGG AGTGCAGATG CTGACAGGAA GCAGCAGTGC 
AGCTTCAAGG GCAAGGACCC AAAGCGTGAC TGTCAAAACT ACATCAAGAT CCTCCTGCCA 
CTCAACAGCA GCCACCTGCT CACCTGTGGC ACGGCCGCCT TCAGCCCCCT GTGTGCTTAC 
ATTCACATAG CGAGCTTTAC TTTAGCCCAA GATGAGGCCG GCAATGTCAT TCTGGAGGAT 
GGCAAGGGTC GTTGTCCCTT TGACCCCAAC TTCAAGTCCA CGGCTCTGGT GGTTGATGGT 
GAGCTGTACA CTGGAACAGT CAGTAGCTTC CAGGGAAACG ACCCAGCCAT TTCCCGGAGC 
CAGAGTTCCC GCCCCACCAA GACTGAGAGC TCCCTCAACT GGCTACAAGA CCCTGCCTTT 
GTGGCCTCGG CCTACGTCCC CGAGAGCCTG GGCAGCCCCA TAGGTGATGA TGATAAGATC 
TACTTCTTCT TCAGCGAGAC GGGCCAGGAG TTTGAGTTCT TTGAGAACAC CATCGTGTCC 
CGAGTTGCCC GAGTCTGTAA GGGCGATGAG GGTGGAGAGC GGGTGTTGCA GCAACGCTGG 
ACCTCCTTTC TCAAGGCTCA GCTCCTGTGC TCCCGGCCTG ATGATGGCTT TCCCTTTAAC 
GTGCTACAAG ATGTCTTCAC CCTGAACCCC AACCCTCAGG ATTGGCGCAA GACCCTTTTC 
TATGGGGTCT TTACCTCCCA GTGGCACAGA GGGACCACAG AAGGCTCTGC CATCTGCGTC 
TTCACCATGA ATGATGTGCA GAAGGCCTTT GACGGCCTGT ACAAGAAAGT AAACAGAGAG 
ACACAGCAGT GGTATACCGA GACCCACCAG GTGCCCACAC CGCGGCCGGG AGCGTGCATT 
ACCAACAGTG CCCGGGAACG GAAGATCAAC TCGTCCCTGC AGCTCCCAGA CCGAGTGCTG 
AACTTCCTCA AGGATCACTT CTTGATGGAT GGGCAGGTCC GCAGTCGCCT GCTGCTGCTG 
CAGCCCAGAG CCCGCTACCA GCGTGTGGCT GTGCACCGTG TGCCTGGCCT GCACAGCACT 
TATGATGTCC TATTTCTGGG CACTGGTGAT GGCCGCCTGC ACAAAGCAGT GACCCTGAGC 
TCCAGAGTCC ACATCATTGA GGAGCTGCAG ATCTTCCCTC AAGGACAGCC TGTGCAGAAC 
CTGCTCTTGG ACAGCCATGG GGGACTGTTG TATGCCTCCT CCCATTCCGG GGTGGTGCAA 
GTGCCCGTAG CCAACTGCAG CCTGTACCCA ACCTGTGGAG ACTGCCTCCT GGCTCGAGAC 
CCCTACTGCG CCTGGACTGG CTCTGCCTGC AGGCTCGCTA GCCTCTACCA GCCTGATCTG 
GCCTCCAGGC CATGGACCCA GGACATTGAG GGTGCCAGTG TCAAGGAACT CTGCAAGAAT 
TCCTCATACA AGGCCCGGTT TCTTGTGCCA GGTAAGCCAT GTAAACAAGT CCAGATCCAA 
CCAAACACAG TGAACACCCT GGCCTGCCCA CTCCTCTCAA ACCTGGCCAC TCGGCTCTGG 
GTGCACAATG GAGCCCCAGT CAATGCCTCT GCCTCCTGCC GCGTGTTACC CACCGGGGAC 
CTGCTGCTGG TGGGCAGCCA GCAGGGTTTG GGGGTGTTCC AGTGTTGGTC GATAGAAGAA 
GGATTCCAGC AGCTTGTGGC CAGCTACTGC CCAGAGGTGA TGGAGGAGGG GGTAATGGAC 
CAAAAGAACC AGCGTGATGG TACCCCAGTC ATTATCAACA CATCACGAGT GAGTGCACCG 
GCTGGTGGCA GGGCCAGCTG GGGTGCGGAC AAGTCCTACT GGAATGAATT CCTGGTGATG 
TGTACTCTGT TTGTGTTTGC TATGGTGCTT TTGTTTCTGT TCTTTCTCTA CCGACATCGG 
GATGGCATGA AACTCTTCCT AAAGCAGGGC GAATGTGCCA GTGTGCACCC CAAGACTCGC 
CCTATAGTGC TACCACCTGA GACCCGACCG CTGAATGGTG TCGGCCCTCC TAGCACCCCA 
CTTGACCACC GAGGCTACCA GGCTCTGTCG GATAGCTCCC CAGGGCCCAG AGTCTTCACT 
GAATCAGAGA AGAGGCCACT GAGCATCCAG GACAGCTTTG TAGAGGTGTC TCCCGTGTGT 
CCCCGGCCCC GAGTTCGACT GGGCTCTGAG ATCCGAGACT CTGTGGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Astrogliosis Induced by Brain Injury Is Regulated by Sema4B Phosphorylation(123).
eNeuro2(3)(2015 May-Jun)
Ben-Gigi L,Sweetat S,Besser E,Fellig Y,Wiederhold T,Polakiewicz RD,Behar O

Semaphorin-Plexin Signaling Controls Mitotic Spindle Orientation during Epithelial Morphogenesis and Repair.
Developmental cell33(3)299-313(2015 May)
Xia J,Swiercz JM,Ba??n-Rodr?guez I,Matkovi? I,Federico G,Sun T,Franz T,Brakebusch CH,Kumanogoh A,Friedel RH,Mart?n-Belmonte F,Gr?ne HJ,Offermanns S,Worzfeld T

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Neuron83(2)309-323(2014 Jul)
Thompson CL,Ng L,Menon V,Martinez S,Lee CK,Glattfelder K,Sunkin SM,Henry A,Lau C,Dang C,Garcia-Lopez R,Martinez-Ferre A,Pombero A,Rubenstein JLR,Wakeman WB,Hohmann J,Dee N,Sodt AJ,Young R,Smith K,Nguyen TN,Kidney J,Kuan L,Jeromin A,Kaykas A,Miller J,Page D,Orta G,Bernard A,Riley Z,Smith S,Wohnoutka P,Hawrylycz MJ,Puelles L,Jones AR

An antagonistic interaction between PlexinB2 and Rnd3 controls RhoA activity and cortical neuron migration.
Nature communications53405(2014 Feb)
Azzarelli R,Pacary E,Garg R,Garcez P,van den Berg D,Riou P,Ridley AJ,Friedel RH,Parsons M,Guillemot F

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Sema4b cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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