Rnaseh1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rnaseh1
Entrez Gene ID	19819
Full Name	ribonuclease H1
General protein information	Preferred Names ribonuclease H1 Names ribonuclease H1 RNase H1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 A2
Summary	This gene encodes an endonuclease that specifically degrades the RNA of RNA-DNA hybrids and is necessary for DNA replication and repair. This enzyme is present in both mitochondria and nuclei, which are resulted from translation of a single mRNA with two in-frame initiation start codons. The use of the first start codon produces the mitochondrial isoform and the use of the second start codon produces the nuclear isoform. The production of the mitochondrial isoform is modulated by an upstream open reading frame (uORF) which encodes 7aa in mouse. An alternately spliced transcript variant has been found which is a candidate for nonsense-mediated mRNA decay (NMD). [provided by RefSeq, Nov 2013].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001286865.1	NP_001273794.1	ribonuclease H1 isoform 2
NM_011275.3	NP_035405.2	ribonuclease H1 isoform 1 precursor

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	rnh-1.0	NP_001040786.1
Danio rerio (zebrafish)	rnaseh1	NP_001002659.1
Eremothecium gossypii	AGOS_ABL017C	NP_982930.2
Bos taurus (cattle)	RNASEH1	NP_001039970.1
Mus musculus (house mouse)	Rnaseh1	NP_035405.2
Gallus gallus (chicken)	RNASEH1	NP_990329.1
Drosophila melanogaster (fruit fly)	rnh1	NP_995777.1
Homo sapiens (human)	RNASEH1	NP_002927.2
Pan troglodytes (chimpanzee)	RNASEH1	XP_001150506.1
	RNASEH1	XP_001097079.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008088	XP_317369.4
Xenopus tropicalis (tropical clawed frog)	rnaseh1	NP_001096299.1
Canis lupus familiaris (dog)	RNASEH1	XP_532858.2
Rattus norvegicus (Norway rat)	Rnaseh1	NP_001013115.1

Gene Ontology
Bibliography

Related articles in PubMed

Viable RNaseH1 knockout mice show RNaseH1 is essential for R loop processing, mitochondrial and liver function.
Lima WF, Murray HM, Damle SS, Hart CE, Hung G, De Hoyos CL, Liang XH, Crooke ST
Nucleic acids research44(11)5299-312(2016 06)

Primer retention owing to the absence of RNase H1 is catastrophic for mitochondrial DNA replication.
Holmes JB, Akman G, Wood SR, Sakhuja K, Cerritelli SM, Moss C, Bowmaker MR, Jacobs HT, Crouch RJ, Holt IJ
Proceedings of the National Academy of Sciences of the United States of America112(30)9334-9(2015 Jul)

An upstream open reading frame and the context of the two AUG codons affect the abundance of mitochondrial and nuclear RNase H1.
Suzuki Y, Holmes JB, Cerritelli SM, Sakhuja K, Minczuk M, Holt IJ, Crouch RJ
Molecular and cellular biology30(21)5123-34(2010 Nov)

Ribonuclease H: the enzymes in eukaryotes.
Cerritelli SM, Crouch RJ
The FEBS journal276(6)1494-505(2009 Mar)

Cracking the egg: molecular dynamics and evolutionary aspects of the transition from the fully grown oocyte to embryo.
Evsikov AV, Graber JH, Brockman JM, Hampl A, Holbrook AE, Singh P, Eppig JJ, Solter D, Knowles BB
Genes & development20(19)2713-27(2006 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RNase H1 is necessary for the activity of DNA-like ASOs. During liver regeneration, a clone of hepatocytes that expressed RNase H1 developed and partially restored mitochondrial and liver function.
Title: Viable RNaseH1 knockout mice show RNaseH1 is essential for R loop processing, mitochondrial and liver function.

the essential role of RNase H1 in mitochondrial DNA replication is the removal of primers at the origin of replication
Title: Primer retention owing to the absence of RNase H1 is catastrophic for mitochondrial DNA replication.

translational organization of RNase H1 allows tight control of expression of RNase H1 in mitochondria, where its excess or absence can lead to cell death, without affecting the expression of the nuclear RNase H1
Title: An upstream open reading frame and the context of the two AUG codons affect the abundance of mitochondrial and nuclear RNase H1.

RNase H1 is involved in generation of mitochondrial DNA
Title: Failure to produce mitochondrial DNA results in embryonic lethality in Rnaseh1 null mice.

The following Rnaseh1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rnaseh1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu13993	NM_001286865.1 Latest version!	Mus musculus ribonuclease H1 (Rnaseh1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu14110	NM_011275.3 Latest version!	Mus musculus ribonuclease H1 (Rnaseh1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rnaseh1 ( NM_001286865.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001273794.1 Mus musculus ribonuclease H1 (Rnaseh1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu13993
Clone ID Related Accession (Same CDS sequence)	NM_001286865.1
Accession Version	NM_001286865.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	ribonuclease H1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY274268.1, CA554498.1, BM943446.1, DV662103.1 and AI390696.1. Transcript Variant: This variant (1) encodes two isoforms due to the use of alternative translation initiation codons. The longer isoform (1) is derived from the upstream AUG start codon, while the shorter isoform (2) is derived from the downstream AUG start codon. This RefSeq represents the shorter isoform (2), which is a nuclear protein (see details in PMID: 20823270). ##Evidence-Data-START## Transcript exon combination :: AF048993.1, BI330591.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:20823270) gene product(s) localized to mito. :: PMID: 20823270; reported by MitoCarta regulatory uORF :: PMID: 20823270 ##RefSeq-Attributes-END##

ATGTTCTATG CGGTGAGGAG AGGCCGCAGG ACCGGAGTCT TCCTGAGTTG GAGTGAGTGC 
AAAGCCCAGG TGGACCGGTT TCCTGCTGCC AGGTTTAAGA AATTTGCCAC AGAAGATGAG 
GCCTGGGCCT TTGTCAGGAG CTCTTCAAGC CCGGATGGTT CAAAAGGGCA GGAAAGTGCA 
CATGAGCAGA AGTCACAGGC GAAGACCAGC AAGCGGCCTC GGGAGCCCCT GGGTGAAGGG 
GAAGAACTTC CAGAGCCAGG GCCGAAGCAC ACACGACAGG ACACCGAGCC CTCGGCTGTA 
GTGAGCAAGG ACGCATTTTC TTATATGGGA GAGTCAGTCG TTGTCTACAC GGATGGCTGT 
TGCTCCAGTA ATGGACGGAA GCGGGCACGA GCAGGAATTG GCGTTTACTG GGGCCCAGGC 
CACCCCTTAA ATGTAGGTAT AAGGCTTCCT GGGCGACAGA CAAACCAGAG GGCCGAGATC 
CATGCAGCCT GCAAGGCCAT CATGCAAGCC AAGGCTCAGA ACATCAGCAA GCTGGTTCTG 
TACACAGACA GCATGTTCAC CATCAATGGG ATAACTAACT GGGTTCAGGG CTGGAAGAAG 
AATGGCTGGA GAACAAGTAC AGGGAAAGAT GTGATCAACA AGGAGGACTT CATGGAGCTG 
GACGAGCTCA CTCAGGGCAT GGACATCCAG TGGATGCACA TTCCTGGTCA CTCAGGATTT 
GTGGGCAATG AAGAGGCCGA CAGACTGGCA CGGGAAGGAG CGAAGCAGTC TGAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273794.1
CDS	166..945
Translation	MFYAVRRGRRTGVFLSWSECKAQVDRFPAARFKKFATEDEAWAFVRSSSSPDGSKGQESAHEQKSQAKTSKRPREPLGEGEELPEPGPKHTRQDTEPSAVVSKDAFSYMGESVVVYTDGCCSSNGRKRARAGIGVYWGPGHPLNVGIRLPGRQTNQRAEIHAACKAIMQAKAQNISKLVLYTDSMFTINGITNWVQGWKKNGWRTSTGKDVINKEDFMELDELTQGMDIQWMHIPGHSGFVGNEEADRLAREGAKQSED

Target ORF information:

RefSeq Version	NM_001286865.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ribonuclease H1 (Rnaseh1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286865.1

ORF Insert Sequence:

ATGTTCTATG CGGTGAGGAG AGGCCGCAGG ACCGGAGTCT TCCTGAGTTG GAGTGAGTGC 
AAAGCCCAGG TGGACCGGTT TCCTGCTGCC AGGTTTAAGA AATTTGCCAC AGAAGATGAG 
GCCTGGGCCT TTGTCAGGAG CTCTTCAAGC CCGGATGGTT CAAAAGGGCA GGAAAGTGCA 
CATGAGCAGA AGTCACAGGC GAAGACCAGC AAGCGGCCTC GGGAGCCCCT GGGTGAAGGG 
GAAGAACTTC CAGAGCCAGG GCCGAAGCAC ACACGACAGG ACACCGAGCC CTCGGCTGTA 
GTGAGCAAGG ACGCATTTTC TTATATGGGA GAGTCAGTCG TTGTCTACAC GGATGGCTGT 
TGCTCCAGTA ATGGACGGAA GCGGGCACGA GCAGGAATTG GCGTTTACTG GGGCCCAGGC 
CACCCCTTAA ATGTAGGTAT AAGGCTTCCT GGGCGACAGA CAAACCAGAG GGCCGAGATC 
CATGCAGCCT GCAAGGCCAT CATGCAAGCC AAGGCTCAGA ACATCAGCAA GCTGGTTCTG 
TACACAGACA GCATGTTCAC CATCAATGGG ATAACTAACT GGGTTCAGGG CTGGAAGAAG 
AATGGCTGGA GAACAAGTAC AGGGAAAGAT GTGATCAACA AGGAGGACTT CATGGAGCTG 
GACGAGCTCA CTCAGGGCAT GGACATCCAG TGGATGCACA TTCCTGGTCA CTCAGGATTT 
GTGGGCAATG AAGAGGCCGA CAGACTGGCA CGGGAAGGAG CGAAGCAGTC TGAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rnaseh1 ( NM_011275.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035405.2 Mus musculus ribonuclease H1 (Rnaseh1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu14110
Clone ID Related Accession (Same CDS sequence)	NM_011275.3
Accession Version	NM_011275.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	ribonuclease H1 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY274268.1, CA554498.1, BM943446.1, DV662103.1 and AI390696.1. On Nov 19, 2013 this sequence version replaced NM_011275.2. Transcript Variant: This variant (1) encodes two isoforms due to the use of alternative translation initiation codons. The longer isoform (1) is derived from the upstream AUG start codon, while the shorter isoform (2) is derived from the downstream AUG start codon. This RefSeq represents the longer isoform (1), which is a mitochondrial protein (see details in PMID: 20823270). ##Evidence-Data-START## Transcript exon combination :: AF048993.1, BI330591.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 20823270; reported by MitoCarta regulatory uORF :: PMID: 20823270 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGCTGGC TGCTGCCGCT GTCCCGCACA GTGACACTGG CCGTCGTACG CCTGAGGCGA 
GGCATTTGCG GGCTCGGGAT GTTCTATGCG GTGAGGAGAG GCCGCAGGAC CGGAGTCTTC 
CTGAGTTGGA GTGAGTGCAA AGCCCAGGTG GACCGGTTTC CTGCTGCCAG GTTTAAGAAA 
TTTGCCACAG AAGATGAGGC CTGGGCCTTT GTCAGGAGCT CTTCAAGCCC GGATGGTTCA 
AAAGGGCAGG AAAGTGCACA TGAGCAGAAG TCACAGGCGA AGACCAGCAA GCGGCCTCGG 
GAGCCCCTGG GTGAAGGGGA AGAACTTCCA GAGCCAGGGC CGAAGCACAC ACGACAGGAC 
ACCGAGCCCT CGGCTGTAGT GAGCAAGGAC GCATTTTCTT ATATGGGAGA GTCAGTCGTT 
GTCTACACGG ATGGCTGTTG CTCCAGTAAT GGACGGAAGC GGGCACGAGC AGGAATTGGC 
GTTTACTGGG GCCCAGGCCA CCCCTTAAAT GTAGGTATAA GGCTTCCTGG GCGACAGACA 
AACCAGAGGG CCGAGATCCA TGCAGCCTGC AAGGCCATCA TGCAAGCCAA GGCTCAGAAC 
ATCAGCAAGC TGGTTCTGTA CACAGACAGC ATGTTCACCA TCAATGGGAT AACTAACTGG 
GTTCAGGGCT GGAAGAAGAA TGGCTGGAGA ACAAGTACAG GGAAAGATGT GATCAACAAG 
GAGGACTTCA TGGAGCTGGA CGAGCTCACT CAGGGCATGG ACATCCAGTG GATGCACATT 
CCTGGTCACT CAGGATTTGT GGGCAATGAA GAGGCCGACA GACTGGCACG GGAAGGAGCG 
AAGCAGTCTG AGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035405.2
CDS	88..945
Translation	MRWLLPLSRTVTLAVVRLRRGICGLGMFYAVRRGRRTGVFLSWSECKAQVDRFPAARFKKFATEDEAWAFVRSSSSPDGSKGQESAHEQKSQAKTSKRPREPLGEGEELPEPGPKHTRQDTEPSAVVSKDAFSYMGESVVVYTDGCCSSNGRKRARAGIGVYWGPGHPLNVGIRLPGRQTNQRAEIHAACKAIMQAKAQNISKLVLYTDSMFTINGITNWVQGWKKNGWRTSTGKDVINKEDFMELDELTQGMDIQWMHIPGHSGFVGNEEADRLAREGAKQSED

Target ORF information:

RefSeq Version	NM_011275.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ribonuclease H1 (Rnaseh1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011275.3

ORF Insert Sequence:

ATGCGCTGGC TGCTGCCGCT GTCCCGCACA GTGACACTGG CCGTCGTACG CCTGAGGCGA 
GGCATTTGCG GGCTCGGGAT GTTCTATGCG GTGAGGAGAG GCCGCAGGAC CGGAGTCTTC 
CTGAGTTGGA GTGAGTGCAA AGCCCAGGTG GACCGGTTTC CTGCTGCCAG GTTTAAGAAA 
TTTGCCACAG AAGATGAGGC CTGGGCCTTT GTCAGGAGCT CTTCAAGCCC GGATGGTTCA 
AAAGGGCAGG AAAGTGCACA TGAGCAGAAG TCACAGGCGA AGACCAGCAA GCGGCCTCGG 
GAGCCCCTGG GTGAAGGGGA AGAACTTCCA GAGCCAGGGC CGAAGCACAC ACGACAGGAC 
ACCGAGCCCT CGGCTGTAGT GAGCAAGGAC GCATTTTCTT ATATGGGAGA GTCAGTCGTT 
GTCTACACGG ATGGCTGTTG CTCCAGTAAT GGACGGAAGC GGGCACGAGC AGGAATTGGC 
GTTTACTGGG GCCCAGGCCA CCCCTTAAAT GTAGGTATAA GGCTTCCTGG GCGACAGACA 
AACCAGAGGG CCGAGATCCA TGCAGCCTGC AAGGCCATCA TGCAAGCCAA GGCTCAGAAC 
ATCAGCAAGC TGGTTCTGTA CACAGACAGC ATGTTCACCA TCAATGGGAT AACTAACTGG 
GTTCAGGGCT GGAAGAAGAA TGGCTGGAGA ACAAGTACAG GGAAAGATGT GATCAACAAG 
GAGGACTTCA TGGAGCTGGA CGAGCTCACT CAGGGCATGG ACATCCAGTG GATGCACATT 
CCTGGTCACT CAGGATTTGT GGGCAATGAA GAGGCCGACA GACTGGCACG GGAAGGAGCG 
AAGCAGTCTG AGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Viable RNaseH1 knockout mice show RNaseH1 is essential for R loop processing, mitochondrial and liver function.
Nucleic acids research44(11)5299-312(2016 06)
Lima WF,Murray HM,Damle SS,Hart CE,Hung G,De Hoyos CL,Liang XH,Crooke ST

Primer retention owing to the absence of RNase H1 is catastrophic for mitochondrial DNA replication.
Proceedings of the National Academy of Sciences of the United States of America112(30)9334-9(2015 Jul)
Holmes JB,Akman G,Wood SR,Sakhuja K,Cerritelli SM,Moss C,Bowmaker MR,Jacobs HT,Crouch RJ,Holt IJ

An upstream open reading frame and the context of the two AUG codons affect the abundance of mitochondrial and nuclear RNase H1.
Molecular and cellular biology30(21)5123-34(2010 Nov)
Suzuki Y,Holmes JB,Cerritelli SM,Sakhuja K,Minczuk M,Holt IJ,Crouch RJ

Ribonuclease H: the enzymes in eukaryotes.
The FEBS journal276(6)1494-505(2009 Mar)
Cerritelli SM,Crouch RJ

Cracking the egg: molecular dynamics and evolutionary aspects of the transition from the fully grown oocyte to embryo.
Genes & development20(19)2713-27(2006 Oct)
Evsikov AV,Graber JH,Brockman JM,Hampl A,Holbrook AE,Singh P,Eppig JJ,Solter D,Knowles BB

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Rnaseh1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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