RNASEH1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RNASEH1
Entrez Gene ID	246243
Full Name	ribonuclease H1
Synonyms	H1RNA,PEOB2,RNH1
General protein information	Preferred Names ribonuclease H1 Names ribonuclease H1 ribonuclease H type II
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p25.3
Summary	This gene encodes an endonuclease that specifically degrades the RNA of RNA-DNA hybrids and plays a key role in DNA replication and repair. Alternate in-frame start codon initiation results in the production of alternate isoforms that are directed to the mitochondria or to the nucleus. The production of the mitochondrial isoform is modulated by an upstream open reading frame (uORF). Mutations in this gene have been found in individuals with progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal recessive 2. Alternative splicing results in additional coding and non-coding transcript variants. Pseudogenes of this gene have been defined on chromosomes 2 and 17. [provided by RefSeq, Jul 2017].
Disorder MIM:	604123
Disorder Html:	Progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal recessive 2, 616479 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001286837.1	NP_001273766.1	ribonuclease H1 isoform 3
NM_001286834.1	NP_001273763.1	ribonuclease H1 isoform 2
NM_002936.4	NP_002927.2	ribonuclease H1 isoform 1 precursor
NM_001286837.2	NP_001273766.1	ribonuclease H1 isoform 3
NM_001286837.3	NP_001273766.1	ribonuclease H1 isoform 3
NM_001286834.2	NP_001273763.1	ribonuclease H1 isoform 2
NM_002936.5	NP_002927.2	ribonuclease H1 isoform 1 precursor

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	rnh-1.0	NP_001040786.1
Danio rerio (zebrafish)	rnaseh1	NP_001002659.1
Eremothecium gossypii	AGOS_ABL017C	NP_982930.2
Bos taurus (cattle)	RNASEH1	NP_001039970.1
Mus musculus (house mouse)	Rnaseh1	NP_035405.2
Gallus gallus (chicken)	RNASEH1	NP_990329.1
Drosophila melanogaster (fruit fly)	rnh1	NP_995777.1
Homo sapiens (human)	RNASEH1	NP_002927.2
Pan troglodytes (chimpanzee)	RNASEH1	XP_001150506.1
	RNASEH1	XP_001097079.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008088	XP_317369.4
Xenopus tropicalis (tropical clawed frog)	rnaseh1	NP_001096299.1
Canis lupus familiaris (dog)	RNASEH1	XP_532858.2
Rattus norvegicus (Norway rat)	Rnaseh1	NP_001013115.1

Gene Ontology
Bibliography

Related articles in PubMed

RNASEH1 gene variants are associated with autoimmune type 1 diabetes in Colombia.
Pineda-Trujillo N, Rodr?guez-Acevedo A, Rodr?guez A, Ru?z-Linares A, Bedoya G, Rivera A, Alfaro JM
Journal of endocrinological investigation(2017 Dec)

RNase H sequence preferences influence antisense oligonucleotide efficiency.
Kielpinski LJ, Hagedorn PH, Lindow M, Vinther J
Nucleic acids research45(22)12932-12944(2017 Dec)

Human ribonuclease H1 resolves R-loops and thereby enables progression of the DNA replication fork.
Parajuli S, Teasley DC, Murali B, Jackson J, Vindigni A, Stewart SA
The Journal of biological chemistry292(37)15216-15224(2017 09)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that the catalytic domains of E. coli and human RNase H have nearly identical sequence preferences, which correlate with the efficiency of RNase H-recruiting antisense oligonucleotides.
Title: RNase H sequence preferences influence antisense oligonucleotide efficiency.

Data suggest that ribonuclease H1 (RNASEH1) plays important role in replication fork movement by resolving R-loops (RNA-DNA hybrids); RNASEH1 depletion results in accumulation of RNA-DNA hybrids, slowing of replication forks, and increased DNA damage; RNASEH1 appears to contribute to genome stability and preserves telomere integrity.
Title: Human ribonuclease H1 resolves R-loops and thereby enables progression of the DNA replication fork.

RPA is a sensor of R loops and a regulator of RNaseH1, extending the versatile role of RPA in suppression of genomic instability.
Title: Functions of Replication Protein A as a Sensor of R Loops and a Regulator of RNaseH1.

found that the 3' fragments of target pre-mRNA generated by ASO were almost completely degraded from their 5' ends by nuclear XRN2 after RNase H1-mediated cleavage
Title: XRN2 is required for the degradation of target RNAs by RNase H1-dependent antisense oligonucleotides.

Altered RNaseH1 has a reduced capability to remove the RNA from RNA-DNA hybrids leading to impaired mtDNA replication and adult-onset mitochondrial encephalomyopathy.
Title: RNASEH1 Mutations Impair mtDNA Replication and Cause Adult-Onset Mitochondrial Encephalomyopathy.

The following RNASEH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RNASEH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu20534	NM_002936.5 Latest version!	Homo sapiens ribonuclease H1 (RNASEH1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu21231	NM_001286837.3 Latest version!	Homo sapiens ribonuclease H1 (RNASEH1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu21389	NM_001286834.2 Latest version!	Homo sapiens ribonuclease H1 (RNASEH1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
View Old Accession Versions >>
OHu21231	NM_001286837.1	Homo sapiens ribonuclease H1 (RNASEH1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu21231	NM_001286837.2	Homo sapiens ribonuclease H1 (RNASEH1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu21389	NM_001286834.1	Homo sapiens ribonuclease H1 (RNASEH1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu20534	NM_002936.4	Homo sapiens ribonuclease H1 (RNASEH1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RNASEH1 ( NM_002936.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002927.2 Homo sapiens ribonuclease H1 (RNASEH1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20534
Clone ID Related Accession (Same CDS sequence)	NM_002936.4 , NM_002936.5
Accession Version	NM_002936.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-06
Organism	Homo sapiens(Human)
Product	ribonuclease H1 isoform 1 precursor
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA532761.1, BC002973.1, AK075490.1 and DA612116.1. This sequence is a reference standard in the RefSeqGene project. On Nov 19, 2013 this sequence version replaced NM_002936.3. Transcript Variant: This variant (1) encodes two isoforms due to the use of alternative translation initiation codons. The longer isoform (1) is derived from the upstream AUG start codon, while the shorter isoform (2) is derived from the downstream AUG start codon. This RefSeq represents the longer isoform (1), which is a mitochondrial protein (see details in PMID: 20823270). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK075490.1, BC002973.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 20823270; reported by MitoCarta regulatory uORF :: PMID: 20823270 ##RefSeq-Attributes-END##

ATGAGCTGGC TTCTGTTCCT GGCCCACAGA GTCGCCTTGG CCGCCTTGCC CTGCCGCCGC 
GGCTCTCGCG GGTTCGGGAT GTTCTATGCC GTGAGGAGGG GCCGCAAGAC CGGGGTCTTT 
CTGACCTGGA ATGAGTGCAG AGCACAGGTG GACCGGTTTC CTGCTGCCAG ATTTAAGAAG 
TTTGCCACAG AGGATGAGGC CTGGGCCTTT GTCAGGAAAT CTGCAAGCCC GGAAGTTTCA 
GAAGGGCATG AAAATCAACA TGGACAAGAA TCGGAGGCGA AAGCCAGCAA GCGACTCCGT 
GAGCCACTGG ATGGAGATGG ACATGAAAGC GCAGAGCCGT ATGCAAAGCA CATGAAGCCG 
AGCGTGGAGC CGGCGCCTCC AGTTAGCAGA GACACGTTTT CCTACATGGG AGACTTCGTC 
GTCGTCTACA CTGATGGCTG CTGCTCCAGT AATGGGCGTA GAAGGCCGCG AGCAGGAATC 
GGCGTTTACT GGGGGCCAGG CCATCCTTTA AATGTAGGCA TTAGACTTCC TGGGCGGCAG 
ACAAACCAAA GAGCGGAAAT TCATGCAGCC TGCAAAGCCA TTGAACAAGC AAAGACTCAA 
AACATCAATA AACTGGTTCT GTATACAGAC AGTATGTTTA CGATAAATGG TATAACTAAC 
TGGGTTCAAG GTTGGAAGAA AAATGGGTGG AAGACAAGTG CAGGGAAAGA GGTGATCAAC 
AAAGAGGACT TTGTGGCACT GGAGAGGCTT ACCCAGGGGA TGGACATTCA GTGGATGCAT 
GTTCCTGGTC ATTCGGGATT TATAGGCAAT GAAGAAGCTG ACAGATTAGC CAGAGAAGGA 
GCTAAACAAT CGGAAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002927.2
CDS	112..972
Translation	MSWLLFLAHRVALAALPCRRGSRGFGMFYAVRRGRKTGVFLTWNECRAQVDRFPAARFKKFATEDEAWAFVRKSASPEVSEGHENQHGQESEAKASKRLREPLDGDGHESAEPYAKHMKPSVEPAPPVSRDTFSYMGDFVVVYTDGCCSSNGRRRPRAGIGVYWGPGHPLNVGIRLPGRQTNQRAEIHAACKAIEQAKTQNINKLVLYTDSMFTINGITNWVQGWKKNGWKTSAGKEVINKEDFVALERLTQGMDIQWMHVPGHSGFIGNEEADRLAREGAKQSED

Target ORF information:

RefSeq Version	NM_002936.4
Organism	Homo sapiens(Human)
Definition	Homo sapiens ribonuclease H1 (RNASEH1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002936.4

ORF Insert Sequence:

ATGAGCTGGC TTCTGTTCCT GGCCCACAGA GTCGCCTTGG CCGCCTTGCC CTGCCGCCGC 
GGCTCTCGCG GGTTCGGGAT GTTCTATGCC GTGAGGAGGG GCCGCAAGAC CGGGGTCTTT 
CTGACCTGGA ATGAGTGCAG AGCACAGGTG GACCGGTTTC CTGCTGCCAG ATTTAAGAAG 
TTTGCCACAG AGGATGAGGC CTGGGCCTTT GTCAGGAAAT CTGCAAGCCC GGAAGTTTCA 
GAAGGGCATG AAAATCAACA TGGACAAGAA TCGGAGGCGA AAGCCAGCAA GCGACTCCGT 
GAGCCACTGG ATGGAGATGG ACATGAAAGC GCAGAGCCGT ATGCAAAGCA CATGAAGCCG 
AGCGTGGAGC CGGCGCCTCC AGTTAGCAGA GACACGTTTT CCTACATGGG AGACTTCGTC 
GTCGTCTACA CTGATGGCTG CTGCTCCAGT AATGGGCGTA GAAGGCCGCG AGCAGGAATC 
GGCGTTTACT GGGGGCCAGG CCATCCTTTA AATGTAGGCA TTAGACTTCC TGGGCGGCAG 
ACAAACCAAA GAGCGGAAAT TCATGCAGCC TGCAAAGCCA TTGAACAAGC AAAGACTCAA 
AACATCAATA AACTGGTTCT GTATACAGAC AGTATGTTTA CGATAAATGG TATAACTAAC 
TGGGTTCAAG GTTGGAAGAA AAATGGGTGG AAGACAAGTG CAGGGAAAGA GGTGATCAAC 
AAAGAGGACT TTGTGGCACT GGAGAGGCTT ACCCAGGGGA TGGACATTCA GTGGATGCAT 
GTTCCTGGTC ATTCGGGATT TATAGGCAAT GAAGAAGCTG ACAGATTAGC CAGAGAAGGA 
GCTAAACAAT CGGAAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNASEH1 ( NM_002936.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_002927.2 Homo sapiens ribonuclease H1 (RNASEH1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20534
Clone ID Related Accession (Same CDS sequence)	NM_002936.4 , NM_002936.5
Accession Version	NM_002936.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	ribonuclease H1 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA532761.1, BC002973.1, AK075490.1, DA612116.1 and AC108488.4. This sequence is a reference standard in the RefSeqGene project. On Jul 22, 2017 this sequence version replaced NM_002936.4. Transcript Variant: This variant (1) encodes two isoforms due to the use of alternative translation initiation codons. The longer isoform (1) is derived from the upstream AUG start codon, while the shorter isoform (2) is derived from the downstream AUG start codon. This RefSeq represents the longer isoform (1), which is a mitochondrial protein (see details in PMID: 20823270). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.44896.1, SRR1660807.157882.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 20823270; reported by MitoCarta RefSeq Select criteria :: based on conservation, expression, longest protein regulatory uORF :: PMID: 20823270 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGCTGGC TTCTGTTCCT GGCCCACAGA GTCGCCTTGG CCGCCTTGCC CTGCCGCCGC 
GGCTCTCGCG GGTTCGGGAT GTTCTATGCC GTGAGGAGGG GCCGCAAGAC CGGGGTCTTT 
CTGACCTGGA ATGAGTGCAG AGCACAGGTG GACCGGTTTC CTGCTGCCAG ATTTAAGAAG 
TTTGCCACAG AGGATGAGGC CTGGGCCTTT GTCAGGAAAT CTGCAAGCCC GGAAGTTTCA 
GAAGGGCATG AAAATCAACA TGGACAAGAA TCGGAGGCGA AAGCCAGCAA GCGACTCCGT 
GAGCCACTGG ATGGAGATGG ACATGAAAGC GCAGAGCCGT ATGCAAAGCA CATGAAGCCG 
AGCGTGGAGC CGGCGCCTCC AGTTAGCAGA GACACGTTTT CCTACATGGG AGACTTCGTC 
GTCGTCTACA CTGATGGCTG CTGCTCCAGT AATGGGCGTA GAAGGCCGCG AGCAGGAATC 
GGCGTTTACT GGGGGCCAGG CCATCCTTTA AATGTAGGCA TTAGACTTCC TGGGCGGCAG 
ACAAACCAAA GAGCGGAAAT TCATGCAGCC TGCAAAGCCA TTGAACAAGC AAAGACTCAA 
AACATCAATA AACTGGTTCT GTATACAGAC AGTATGTTTA CGATAAATGG TATAACTAAC 
TGGGTTCAAG GTTGGAAGAA AAATGGGTGG AAGACAAGTG CAGGGAAAGA GGTGATCAAC 
AAAGAGGACT TTGTGGCACT GGAGAGGCTT ACCCAGGGGA TGGACATTCA GTGGATGCAT 
GTTCCTGGTC ATTCGGGATT TATAGGCAAT GAAGAAGCTG ACAGATTAGC CAGAGAAGGA 
GCTAAACAAT CGGAAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002927.2
CDS	112..972
Translation	MSWLLFLAHRVALAALPCRRGSRGFGMFYAVRRGRKTGVFLTWNECRAQVDRFPAARFKKFATEDEAWAFVRKSASPEVSEGHENQHGQESEAKASKRLREPLDGDGHESAEPYAKHMKPSVEPAPPVSRDTFSYMGDFVVVYTDGCCSSNGRRRPRAGIGVYWGPGHPLNVGIRLPGRQTNQRAEIHAACKAIEQAKTQNINKLVLYTDSMFTINGITNWVQGWKKNGWKTSAGKEVINKEDFVALERLTQGMDIQWMHVPGHSGFIGNEEADRLAREGAKQSED

Target ORF information:

RefSeq Version	NM_002936.5
Organism	Homo sapiens(human)
Definition	Homo sapiens ribonuclease H1 (RNASEH1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002936.5

ORF Insert Sequence:

ATGAGCTGGC TTCTGTTCCT GGCCCACAGA GTCGCCTTGG CCGCCTTGCC CTGCCGCCGC 
GGCTCTCGCG GGTTCGGGAT GTTCTATGCC GTGAGGAGGG GCCGCAAGAC CGGGGTCTTT 
CTGACCTGGA ATGAGTGCAG AGCACAGGTG GACCGGTTTC CTGCTGCCAG ATTTAAGAAG 
TTTGCCACAG AGGATGAGGC CTGGGCCTTT GTCAGGAAAT CTGCAAGCCC GGAAGTTTCA 
GAAGGGCATG AAAATCAACA TGGACAAGAA TCGGAGGCGA AAGCCAGCAA GCGACTCCGT 
GAGCCACTGG ATGGAGATGG ACATGAAAGC GCAGAGCCGT ATGCAAAGCA CATGAAGCCG 
AGCGTGGAGC CGGCGCCTCC AGTTAGCAGA GACACGTTTT CCTACATGGG AGACTTCGTC 
GTCGTCTACA CTGATGGCTG CTGCTCCAGT AATGGGCGTA GAAGGCCGCG AGCAGGAATC 
GGCGTTTACT GGGGGCCAGG CCATCCTTTA AATGTAGGCA TTAGACTTCC TGGGCGGCAG 
ACAAACCAAA GAGCGGAAAT TCATGCAGCC TGCAAAGCCA TTGAACAAGC AAAGACTCAA 
AACATCAATA AACTGGTTCT GTATACAGAC AGTATGTTTA CGATAAATGG TATAACTAAC 
TGGGTTCAAG GTTGGAAGAA AAATGGGTGG AAGACAAGTG CAGGGAAAGA GGTGATCAAC 
AAAGAGGACT TTGTGGCACT GGAGAGGCTT ACCCAGGGGA TGGACATTCA GTGGATGCAT 
GTTCCTGGTC ATTCGGGATT TATAGGCAAT GAAGAAGCTG ACAGATTAGC CAGAGAAGGA 
GCTAAACAAT CGGAAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNASEH1 ( NM_001286837.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273766.1 Homo sapiens ribonuclease H1 (RNASEH1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu21231
Clone ID Related Accession (Same CDS sequence)	NM_001286837.1 , NM_001286837.2 , NM_001286837.3
Accession Version	NM_001286837.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-06
Organism	Homo sapiens(Human)
Product	ribonuclease H1 isoform 3
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA532761.1, AK096913.1, BC002973.1, AK075490.1 and DA612116.1. On Nov 19, 2013 this sequence version replaced XM_005263850.1. Transcript Variant: This variant (2) has an alternate splice site in the 5' region, which results in translation initiation at a downstream start codon, compared to variant 1. The resulting isoform (3) has a shorter N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK096913.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 20823270; reported by MitoCarta ##RefSeq-Attributes-END##

ATGAAGCCGA GCGTGGAGCC GGCGCCTCCA GTTAGCAGAG ACACGTTTTC CTACATGGGA 
GACTTCGTCG TCGTCTACAC TGATGGCTGC TGCTCCAGTA ATGGGCGTAG AAGGCCGCGA 
GCAGGAATCG GCGTTTACTG GGGGCCAGGC CATCCTTTAA ATGTAGGCAT TAGACTTCCT 
GGGCGGCAGA CAAACCAAAG AGCGGAAATT CATGCAGCCT GCAAAGCCAT TGAACAAGCA 
AAGACTCAAA ACATCAATAA ACTGGTTCTG TATACAGACA GTATGTTTAC GATAAATGGT 
ATAACTAACT GGGTTCAAGG TTGGAAGAAA AATGGGTGGA AGACAAGTGC AGGGAAAGAG 
GTGATCAACA AAGAGGACTT TGTGGCACTG GAGAGGCTTA CCCAGGGGAT GGACATTCAG 
TGGATGCATG TTCCTGGTCA TTCGGGATTT ATAGGCAATG AAGAAGCTGA CAGATTAGCC 
AGAGAAGGAG CTAAACAATC GGAAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273766.1
CDS	701..1210
Translation	MKPSVEPAPPVSRDTFSYMGDFVVVYTDGCCSSNGRRRPRAGIGVYWGPGHPLNVGIRLPGRQTNQRAEIHAACKAIEQAKTQNINKLVLYTDSMFTINGITNWVQGWKKNGWKTSAGKEVINKEDFVALERLTQGMDIQWMHVPGHSGFIGNEEADRLAREGAKQSED

Target ORF information:

RefSeq Version	NM_001286837.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens ribonuclease H1 (RNASEH1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286837.1

ORF Insert Sequence:

ATGAAGCCGA GCGTGGAGCC GGCGCCTCCA GTTAGCAGAG ACACGTTTTC CTACATGGGA 
GACTTCGTCG TCGTCTACAC TGATGGCTGC TGCTCCAGTA ATGGGCGTAG AAGGCCGCGA 
GCAGGAATCG GCGTTTACTG GGGGCCAGGC CATCCTTTAA ATGTAGGCAT TAGACTTCCT 
GGGCGGCAGA CAAACCAAAG AGCGGAAATT CATGCAGCCT GCAAAGCCAT TGAACAAGCA 
AAGACTCAAA ACATCAATAA ACTGGTTCTG TATACAGACA GTATGTTTAC GATAAATGGT 
ATAACTAACT GGGTTCAAGG TTGGAAGAAA AATGGGTGGA AGACAAGTGC AGGGAAAGAG 
GTGATCAACA AAGAGGACTT TGTGGCACTG GAGAGGCTTA CCCAGGGGAT GGACATTCAG 
TGGATGCATG TTCCTGGTCA TTCGGGATTT ATAGGCAATG AAGAAGCTGA CAGATTAGCC 
AGAGAAGGAG CTAAACAATC GGAAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNASEH1 ( NM_001286837.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273766.1 Homo sapiens ribonuclease H1 (RNASEH1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu21231
Clone ID Related Accession (Same CDS sequence)	NM_001286837.1 , NM_001286837.2 , NM_001286837.3
Accession Version	NM_001286837.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-21
Organism	Homo sapiens(human)
Product	ribonuclease H1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA532761.1, AK096913.1, BC002973.1, AK075490.1, DA612116.1 and AC108488.4. On Jul 22, 2017 this sequence version replaced NM_001286837.1. Transcript Variant: This variant (2) has an alternate splice site in the 5' region, which results in translation initiation at a downstream start codon, compared to variant 1. The resulting isoform (3) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.29080.1, AK096913.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 20823270; reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAAGCCGA GCGTGGAGCC GGCGCCTCCA GTTAGCAGAG ACACGTTTTC CTACATGGGA 
GACTTCGTCG TCGTCTACAC TGATGGCTGC TGCTCCAGTA ATGGGCGTAG AAGGCCGCGA 
GCAGGAATCG GCGTTTACTG GGGGCCAGGC CATCCTTTAA ATGTAGGCAT TAGACTTCCT 
GGGCGGCAGA CAAACCAAAG AGCGGAAATT CATGCAGCCT GCAAAGCCAT TGAACAAGCA 
AAGACTCAAA ACATCAATAA ACTGGTTCTG TATACAGACA GTATGTTTAC GATAAATGGT 
ATAACTAACT GGGTTCAAGG TTGGAAGAAA AATGGGTGGA AGACAAGTGC AGGGAAAGAG 
GTGATCAACA AAGAGGACTT TGTGGCACTG GAGAGGCTTA CCCAGGGGAT GGACATTCAG 
TGGATGCATG TTCCTGGTCA TTCGGGATTT ATAGGCAATG AAGAAGCTGA CAGATTAGCC 
AGAGAAGGAG CTAAACAATC GGAAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273766.1
CDS	701..1210
Translation	MKPSVEPAPPVSRDTFSYMGDFVVVYTDGCCSSNGRRRPRAGIGVYWGPGHPLNVGIRLPGRQTNQRAEIHAACKAIEQAKTQNINKLVLYTDSMFTINGITNWVQGWKKNGWKTSAGKEVINKEDFVALERLTQGMDIQWMHVPGHSGFIGNEEADRLAREGAKQSED

Target ORF information:

RefSeq Version	NM_001286837.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ribonuclease H1 (RNASEH1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286837.2

ORF Insert Sequence:

ATGAAGCCGA GCGTGGAGCC GGCGCCTCCA GTTAGCAGAG ACACGTTTTC CTACATGGGA 
GACTTCGTCG TCGTCTACAC TGATGGCTGC TGCTCCAGTA ATGGGCGTAG AAGGCCGCGA 
GCAGGAATCG GCGTTTACTG GGGGCCAGGC CATCCTTTAA ATGTAGGCAT TAGACTTCCT 
GGGCGGCAGA CAAACCAAAG AGCGGAAATT CATGCAGCCT GCAAAGCCAT TGAACAAGCA 
AAGACTCAAA ACATCAATAA ACTGGTTCTG TATACAGACA GTATGTTTAC GATAAATGGT 
ATAACTAACT GGGTTCAAGG TTGGAAGAAA AATGGGTGGA AGACAAGTGC AGGGAAAGAG 
GTGATCAACA AAGAGGACTT TGTGGCACTG GAGAGGCTTA CCCAGGGGAT GGACATTCAG 
TGGATGCATG TTCCTGGTCA TTCGGGATTT ATAGGCAATG AAGAAGCTGA CAGATTAGCC 
AGAGAAGGAG CTAAACAATC GGAAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNASEH1 ( NM_001286837.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273766.1 Homo sapiens ribonuclease H1 (RNASEH1), transcript variant 2, mRNA.

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CloneID	OHu21231
Clone ID Related Accession (Same CDS sequence)	NM_001286837.1 , NM_001286837.2 , NM_001286837.3
Accession Version	NM_001286837.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	ribonuclease H1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA532761.1, AK096913.1, BC002973.1, AK075490.1, DA612116.1 and AC108488.4. On Jun 2, 2019 this sequence version replaced NM_001286837.2. Transcript Variant: This variant (2) has an alternate splice site in the 5' region, which results in translation initiation at a downstream start codon, compared to variant 1. The resulting isoform (3) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.29080.1, AK096913.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 20823270; reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAAGCCGA GCGTGGAGCC GGCGCCTCCA GTTAGCAGAG ACACGTTTTC CTACATGGGA 
GACTTCGTCG TCGTCTACAC TGATGGCTGC TGCTCCAGTA ATGGGCGTAG AAGGCCGCGA 
GCAGGAATCG GCGTTTACTG GGGGCCAGGC CATCCTTTAA ATGTAGGCAT TAGACTTCCT 
GGGCGGCAGA CAAACCAAAG AGCGGAAATT CATGCAGCCT GCAAAGCCAT TGAACAAGCA 
AAGACTCAAA ACATCAATAA ACTGGTTCTG TATACAGACA GTATGTTTAC GATAAATGGT 
ATAACTAACT GGGTTCAAGG TTGGAAGAAA AATGGGTGGA AGACAAGTGC AGGGAAAGAG 
GTGATCAACA AAGAGGACTT TGTGGCACTG GAGAGGCTTA CCCAGGGGAT GGACATTCAG 
TGGATGCATG TTCCTGGTCA TTCGGGATTT ATAGGCAATG AAGAAGCTGA CAGATTAGCC 
AGAGAAGGAG CTAAACAATC GGAAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273766.1
CDS	663..1172
Translation	MKPSVEPAPPVSRDTFSYMGDFVVVYTDGCCSSNGRRRPRAGIGVYWGPGHPLNVGIRLPGRQTNQRAEIHAACKAIEQAKTQNINKLVLYTDSMFTINGITNWVQGWKKNGWKTSAGKEVINKEDFVALERLTQGMDIQWMHVPGHSGFIGNEEADRLAREGAKQSED

Target ORF information:

RefSeq Version	NM_001286837.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ribonuclease H1 (RNASEH1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286837.3

ORF Insert Sequence:

ATGAAGCCGA GCGTGGAGCC GGCGCCTCCA GTTAGCAGAG ACACGTTTTC CTACATGGGA 
GACTTCGTCG TCGTCTACAC TGATGGCTGC TGCTCCAGTA ATGGGCGTAG AAGGCCGCGA 
GCAGGAATCG GCGTTTACTG GGGGCCAGGC CATCCTTTAA ATGTAGGCAT TAGACTTCCT 
GGGCGGCAGA CAAACCAAAG AGCGGAAATT CATGCAGCCT GCAAAGCCAT TGAACAAGCA 
AAGACTCAAA ACATCAATAA ACTGGTTCTG TATACAGACA GTATGTTTAC GATAAATGGT 
ATAACTAACT GGGTTCAAGG TTGGAAGAAA AATGGGTGGA AGACAAGTGC AGGGAAAGAG 
GTGATCAACA AAGAGGACTT TGTGGCACTG GAGAGGCTTA CCCAGGGGAT GGACATTCAG 
TGGATGCATG TTCCTGGTCA TTCGGGATTT ATAGGCAATG AAGAAGCTGA CAGATTAGCC 
AGAGAAGGAG CTAAACAATC GGAAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNASEH1 ( NM_001286834.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273763.1 Homo sapiens ribonuclease H1 (RNASEH1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21389
Clone ID Related Accession (Same CDS sequence)	NM_001286834.1 , NM_001286834.2
Accession Version	NM_001286834.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-06
Organism	Homo sapiens(Human)
Product	ribonuclease H1 isoform 2
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA532761.1, BC002973.1, AK075490.1 and DA612116.1. Transcript Variant: This variant (1) encodes two isoforms due to the use of alternative translation initiation codons. The longer isoform (1) is derived from the upstream AUG start codon, while the shorter isoform (2) is derived from the downstream AUG start codon. This RefSeq represents the shorter isoform (2), which is a nuclear protein (see details in PMID: 20823270). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK075490.1, BC002973.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:20823270) gene product(s) localized to mito. :: PMID: 20823270; reported by MitoCarta regulatory uORF :: PMID: 20823270 ##RefSeq-Attributes-END##

ATGTTCTATG CCGTGAGGAG GGGCCGCAAG ACCGGGGTCT TTCTGACCTG GAATGAGTGC 
AGAGCACAGG TGGACCGGTT TCCTGCTGCC AGATTTAAGA AGTTTGCCAC AGAGGATGAG 
GCCTGGGCCT TTGTCAGGAA ATCTGCAAGC CCGGAAGTTT CAGAAGGGCA TGAAAATCAA 
CATGGACAAG AATCGGAGGC GAAAGCCAGC AAGCGACTCC GTGAGCCACT GGATGGAGAT 
GGACATGAAA GCGCAGAGCC GTATGCAAAG CACATGAAGC CGAGCGTGGA GCCGGCGCCT 
CCAGTTAGCA GAGACACGTT TTCCTACATG GGAGACTTCG TCGTCGTCTA CACTGATGGC 
TGCTGCTCCA GTAATGGGCG TAGAAGGCCG CGAGCAGGAA TCGGCGTTTA CTGGGGGCCA 
GGCCATCCTT TAAATGTAGG CATTAGACTT CCTGGGCGGC AGACAAACCA AAGAGCGGAA 
ATTCATGCAG CCTGCAAAGC CATTGAACAA GCAAAGACTC AAAACATCAA TAAACTGGTT 
CTGTATACAG ACAGTATGTT TACGATAAAT GGTATAACTA ACTGGGTTCA AGGTTGGAAG 
AAAAATGGGT GGAAGACAAG TGCAGGGAAA GAGGTGATCA ACAAAGAGGA CTTTGTGGCA 
CTGGAGAGGC TTACCCAGGG GATGGACATT CAGTGGATGC ATGTTCCTGG TCATTCGGGA 
TTTATAGGCA ATGAAGAAGC TGACAGATTA GCCAGAGAAG GAGCTAAACA ATCGGAAGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273763.1
CDS	190..972
Translation	MFYAVRRGRKTGVFLTWNECRAQVDRFPAARFKKFATEDEAWAFVRKSASPEVSEGHENQHGQESEAKASKRLREPLDGDGHESAEPYAKHMKPSVEPAPPVSRDTFSYMGDFVVVYTDGCCSSNGRRRPRAGIGVYWGPGHPLNVGIRLPGRQTNQRAEIHAACKAIEQAKTQNINKLVLYTDSMFTINGITNWVQGWKKNGWKTSAGKEVINKEDFVALERLTQGMDIQWMHVPGHSGFIGNEEADRLAREGAKQSED

Target ORF information:

RefSeq Version	NM_001286834.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens ribonuclease H1 (RNASEH1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286834.1

ORF Insert Sequence:

ATGTTCTATG CCGTGAGGAG GGGCCGCAAG ACCGGGGTCT TTCTGACCTG GAATGAGTGC 
AGAGCACAGG TGGACCGGTT TCCTGCTGCC AGATTTAAGA AGTTTGCCAC AGAGGATGAG 
GCCTGGGCCT TTGTCAGGAA ATCTGCAAGC CCGGAAGTTT CAGAAGGGCA TGAAAATCAA 
CATGGACAAG AATCGGAGGC GAAAGCCAGC AAGCGACTCC GTGAGCCACT GGATGGAGAT 
GGACATGAAA GCGCAGAGCC GTATGCAAAG CACATGAAGC CGAGCGTGGA GCCGGCGCCT 
CCAGTTAGCA GAGACACGTT TTCCTACATG GGAGACTTCG TCGTCGTCTA CACTGATGGC 
TGCTGCTCCA GTAATGGGCG TAGAAGGCCG CGAGCAGGAA TCGGCGTTTA CTGGGGGCCA 
GGCCATCCTT TAAATGTAGG CATTAGACTT CCTGGGCGGC AGACAAACCA AAGAGCGGAA 
ATTCATGCAG CCTGCAAAGC CATTGAACAA GCAAAGACTC AAAACATCAA TAAACTGGTT 
CTGTATACAG ACAGTATGTT TACGATAAAT GGTATAACTA ACTGGGTTCA AGGTTGGAAG 
AAAAATGGGT GGAAGACAAG TGCAGGGAAA GAGGTGATCA ACAAAGAGGA CTTTGTGGCA 
CTGGAGAGGC TTACCCAGGG GATGGACATT CAGTGGATGC ATGTTCCTGG TCATTCGGGA 
TTTATAGGCA ATGAAGAAGC TGACAGATTA GCCAGAGAAG GAGCTAAACA ATCGGAAGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNASEH1 ( NM_001286834.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273763.1 Homo sapiens ribonuclease H1 (RNASEH1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21389
Clone ID Related Accession (Same CDS sequence)	NM_001286834.1 , NM_001286834.2
Accession Version	NM_001286834.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Homo sapiens(human)
Product	ribonuclease H1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA532761.1, BC002973.1, AK075490.1, DA612116.1 and AC108488.4. On Jul 22, 2017 this sequence version replaced NM_001286834.1. Transcript Variant: This variant (1) encodes two isoforms due to the use of alternative translation initiation codons. The longer isoform (1) is derived from the upstream AUG start codon, while the shorter isoform (2) is derived from the downstream AUG start codon. This RefSeq represents the shorter isoform (2), which is a nuclear protein (see details in PMID: 20823270). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.44896.1, SRR1660807.157882.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:20823270) gene product(s) localized to mito. :: PMID: 20823270; reported by MitoCarta regulatory uORF :: PMID: 20823270 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTCTATG CCGTGAGGAG GGGCCGCAAG ACCGGGGTCT TTCTGACCTG GAATGAGTGC 
AGAGCACAGG TGGACCGGTT TCCTGCTGCC AGATTTAAGA AGTTTGCCAC AGAGGATGAG 
GCCTGGGCCT TTGTCAGGAA ATCTGCAAGC CCGGAAGTTT CAGAAGGGCA TGAAAATCAA 
CATGGACAAG AATCGGAGGC GAAAGCCAGC AAGCGACTCC GTGAGCCACT GGATGGAGAT 
GGACATGAAA GCGCAGAGCC GTATGCAAAG CACATGAAGC CGAGCGTGGA GCCGGCGCCT 
CCAGTTAGCA GAGACACGTT TTCCTACATG GGAGACTTCG TCGTCGTCTA CACTGATGGC 
TGCTGCTCCA GTAATGGGCG TAGAAGGCCG CGAGCAGGAA TCGGCGTTTA CTGGGGGCCA 
GGCCATCCTT TAAATGTAGG CATTAGACTT CCTGGGCGGC AGACAAACCA AAGAGCGGAA 
ATTCATGCAG CCTGCAAAGC CATTGAACAA GCAAAGACTC AAAACATCAA TAAACTGGTT 
CTGTATACAG ACAGTATGTT TACGATAAAT GGTATAACTA ACTGGGTTCA AGGTTGGAAG 
AAAAATGGGT GGAAGACAAG TGCAGGGAAA GAGGTGATCA ACAAAGAGGA CTTTGTGGCA 
CTGGAGAGGC TTACCCAGGG GATGGACATT CAGTGGATGC ATGTTCCTGG TCATTCGGGA 
TTTATAGGCA ATGAAGAAGC TGACAGATTA GCCAGAGAAG GAGCTAAACA ATCGGAAGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273763.1
CDS	190..972
Translation	MFYAVRRGRKTGVFLTWNECRAQVDRFPAARFKKFATEDEAWAFVRKSASPEVSEGHENQHGQESEAKASKRLREPLDGDGHESAEPYAKHMKPSVEPAPPVSRDTFSYMGDFVVVYTDGCCSSNGRRRPRAGIGVYWGPGHPLNVGIRLPGRQTNQRAEIHAACKAIEQAKTQNINKLVLYTDSMFTINGITNWVQGWKKNGWKTSAGKEVINKEDFVALERLTQGMDIQWMHVPGHSGFIGNEEADRLAREGAKQSED

Target ORF information:

RefSeq Version	NM_001286834.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ribonuclease H1 (RNASEH1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286834.2

ORF Insert Sequence:

ATGTTCTATG CCGTGAGGAG GGGCCGCAAG ACCGGGGTCT TTCTGACCTG GAATGAGTGC 
AGAGCACAGG TGGACCGGTT TCCTGCTGCC AGATTTAAGA AGTTTGCCAC AGAGGATGAG 
GCCTGGGCCT TTGTCAGGAA ATCTGCAAGC CCGGAAGTTT CAGAAGGGCA TGAAAATCAA 
CATGGACAAG AATCGGAGGC GAAAGCCAGC AAGCGACTCC GTGAGCCACT GGATGGAGAT 
GGACATGAAA GCGCAGAGCC GTATGCAAAG CACATGAAGC CGAGCGTGGA GCCGGCGCCT 
CCAGTTAGCA GAGACACGTT TTCCTACATG GGAGACTTCG TCGTCGTCTA CACTGATGGC 
TGCTGCTCCA GTAATGGGCG TAGAAGGCCG CGAGCAGGAA TCGGCGTTTA CTGGGGGCCA 
GGCCATCCTT TAAATGTAGG CATTAGACTT CCTGGGCGGC AGACAAACCA AAGAGCGGAA 
ATTCATGCAG CCTGCAAAGC CATTGAACAA GCAAAGACTC AAAACATCAA TAAACTGGTT 
CTGTATACAG ACAGTATGTT TACGATAAAT GGTATAACTA ACTGGGTTCA AGGTTGGAAG 
AAAAATGGGT GGAAGACAAG TGCAGGGAAA GAGGTGATCA ACAAAGAGGA CTTTGTGGCA 
CTGGAGAGGC TTACCCAGGG GATGGACATT CAGTGGATGC ATGTTCCTGG TCATTCGGGA 
TTTATAGGCA ATGAAGAAGC TGACAGATTA GCCAGAGAAG GAGCTAAACA ATCGGAAGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RNASEH1 gene variants are associated with autoimmune type 1 diabetes in Colombia.
Journal of endocrinological investigation(2017 Dec)
Pineda-Trujillo N,Rodr?guez-Acevedo A,Rodr?guez A,Ru?z-Linares A,Bedoya G,Rivera A,Alfaro JM

RNase H sequence preferences influence antisense oligonucleotide efficiency.
Nucleic acids research45(22)12932-12944(2017 Dec)
Kielpinski LJ,Hagedorn PH,Lindow M,Vinther J

Human ribonuclease H1 resolves R-loops and thereby enables progression of the DNA replication fork.
The Journal of biological chemistry292(37)15216-15224(2017 09)
Parajuli S,Teasley DC,Murali B,Jackson J,Vindigni A,Stewart SA

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RNASEH1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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