Arhgdia cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Arhgdia
Entrez Gene ID	192662
Full Name	Rho GDP dissociation inhibitor (GDI) alpha
Synonyms	5330430M07Rik,Gdi-1,Rho-GDI,RhoDGI,RhoGDI-1
General protein information	Preferred Names Rho GDP dissociation inhibitor (GDI) alpha Names rho GDP-dissociation inhibitor 1 Rho GDI alpha rho GDI 1 rho-GDI alpha
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 E2

mRNA and Protein(s)

mRNA	Protein	Name
XM_006532549.3	XP_006532612.1	rho GDP-dissociation inhibitor 1 isoform X1
NM_133796.7	NP_598557.3	rho GDP-dissociation inhibitor 1
NM_133796.8	NP_598557.3	rho GDP-dissociation inhibitor 1
XM_006532549.2	XP_006532612.1	rho GDP-dissociation inhibitor 1 isoform X1
NM_001363425.1	NP_001350354.1	rho GDP-dissociation inhibitor 1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	ARHGDIA	NP_001074340.1
Kluyveromyces lactis	KLLA0F05313g	XP_455321.1
Homo sapiens (human)	ARHGDIA	NP_001172006.1
Pan troglodytes (chimpanzee)	ARHGDIA	NP_001233363.1
Rattus norvegicus (Norway rat)	Arhgdia	NP_001007006.1
Caenorhabditis elegans (roundworm)	rhi-1	NP_508774.1
Schizosaccharomyces pombe (fission yeast)	SPAC6F12.06	NP_593292.1
Arabidopsis thaliana (thale cress)	SCN1	NP_187445.1
Oryza sativa (rice)	Os06g0318300	NP_001057502.1
Bos taurus (cattle)	ARHGDIA	NP_788823.1
Mus musculus (house mouse)	Arhgdia	NP_598557.3
Drosophila melanogaster (fruit fly)	RhoGDI	NP_001262080.1
Saccharomyces cerevisiae (baker's yeast)	RDI1	NP_010146.1
Eremothecium gossypii	AGOS_ADR098C	NP_984194.1
Neurospora crassa	NCU06561	XP_962268.2
Magnaporthe oryzae (rice blast fungus)	MGG_01689	XP_003714679.1
Oryza sativa (rice)	Os02g0719000	NP_001047942.1
	ARHGDIA	XP_001112147.1
Canis lupus familiaris (dog)	ARHGDIA	XP_849933.1
Danio rerio (zebrafish)	arhgdia	NP_998626.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012168	XP_320365.2
Xenopus tropicalis (tropical clawed frog)	arhgdia	NP_001004847.1

Gene Ontology
Bibliography

Related articles in PubMed

Impaired ?GDI Function in the X-Linked Intellectual Disability: The Impact on Astroglia Vesicle Dynamics.
Potokar M, Jorga?evski J, Lacovich V, Kreft M, Vardjan N, Bianchi V, D'Adamo P, Zorec R
Molecular neurobiology54(4)2458-2468(2017 05)

Disease-causing mutations of RhoGDI? induce Rac1 hyperactivation in podocytes.
Auguste D, Maier M, Baldwin C, Aoudjit L, Robins R, Gupta IR, Takano T
Small GTPases7(2)107-21(2016 Apr)

Protein tyrosine phosphatase-PEST and ?8 integrin regulate spatiotemporal patterns of RhoGDI1 activation in migrating cells.
Lee HS, Cheerathodi M, Chaki SP, Reyes SB, Zheng Y, Lu Z, Paidassi H, DerMardirossian C, Lacy-Hulbert A, Rivera GM, McCarty JH
Molecular and cellular biology35(8)1401-13(2015 Apr)

Role of Rho GDP dissociation inhibitor ? in control of epithelial sodium channel (ENaC)-mediated sodium reabsorption.
Pavlov TS, Levchenko V, Staruschenko A
The Journal of biological chemistry289(41)28651-9(2014 Oct)

Coronin-1C and RCC2 guide mesenchymal migration by trafficking Rac1 and controlling GEF exposure.
Williamson RC, Cowell CA, Hammond CL, Bergen DJ, Roper JA, Feng Y, Rendall TC, Race PR, Bass MD
Journal of cell science127(Pt 19)4292-307(2014 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The siRNA-mediated suppression of expression of alphaGDI especially affected vesicle dynamics.
Title: Impaired ?GDI Function in the X-Linked Intellectual Disability: The Impact on Astroglia Vesicle Dynamics.

GDIalpha has a critical role in suppressing Rac1 activity in mature podocytes
Title: Disease-causing mutations of RhoGDI? induce Rac1 hyperactivation in podocytes.

Integrin beta 8 and PTP-PEST form protein complexes at the leading edge of migrating cells and balance patterns of Rac1 and Cdc42 signaling by controlling the subcellular localization and phosphorylation status of Rho GDP dissociation inhibitor 1.
Title: Protein tyrosine phosphatase-PEST and ?8 integrin regulate spatiotemporal patterns of RhoGDI1 activation in migrating cells.

RhoGDalpha controls ENaC expression and activity via Rac1.
Title: Role of Rho GDP dissociation inhibitor ? in control of epithelial sodium channel (ENaC)-mediated sodium reabsorption.

Results identify a critical role for 14-3-3tau in promoting breast cancer metastasis, in part through binding to and inhibition of RhoGDIalpha, a negative regulator of Rho GTPases and a metastasis suppressor.
Title: 14-3-3? promotes breast cancer invasion and metastasis by inhibiting RhoGDI?.

The following Arhgdia gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Arhgdia cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu16256	NM_133796.8 Latest version!	Mus musculus Rho GDP dissociation inhibitor (GDI) alpha (Arhgdia), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu16256	NM_001363425.1 Latest version!	Mus musculus Rho GDP dissociation inhibitor (GDI) alpha (Arhgdia), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu16256	XM_006532549.3 Latest version!	Mus musculus Rho GDP dissociation inhibitor (GDI) alpha (Arhgdia), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
View Old Accession Versions >>
OMu16256	NM_133796.7	Mus musculus Rho GDP dissociation inhibitor (GDI) alpha (Arhgdia), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu16256	XM_006532549.2	Mus musculus Rho GDP dissociation inhibitor (GDI) alpha (Arhgdia), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Arhgdia ( NM_133796.7 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598557.3 Mus musculus Rho GDP dissociation inhibitor (GDI) alpha (Arhgdia), mRNA.

Price & Availability↑

CloneID	OMu16256
Clone ID Related Accession (Same CDS sequence)	XM_006532549.3 , NM_133796.7 , NM_133796.8 , XM_006532549.2 , NM_001363425.1
Accession Version	NM_133796.7	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Mus musculus(house mouse)
Product	rho GDP-dissociation inhibitor 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ091996.1, AK165241.1 and AL663030.12. On Dec 17, 2009 this sequence version replaced NM_133796.6. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK170718.1, AK165241.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGAAC AGGAACCCAC TGCTGAGCAG CTTGCGCAGA TAGCTGCAGA GAATGAGGAA 
GATGAGCACT CTGTGAACTA CAAGCCTCCA GCCCAGAAGA GCATCCAGGA GATCCAGGAA 
CTGGACAAGG ACGATGAAAG CCTCCGAAAG TACAAGGAGG CCCTGCTGGG CCGAGTAGCT 
GTCTCTGCAG ACCCCAACGT CCCCAATGTC ATTGTGACTC GACTGACCTT GGTATGCAGC 
ACTGCCCCAG GCCCTCTGGA ACTGGACCTG ACAGGTGATC TGGAGAGCTT CAAGAAACAG 
TCGTTTGTCT TGAAGGAAGG TGTGGAGTAC CGGATAAAAA TCTCTTTCCG GGTGAACAGA 
GAGATCGTGT CCGGCATGAA GTACATCCAA CACACATACA GGAAAGGGGT CAAAATTGAC 
AAGACTGACT ACATGGTCGG GAGCTATGGG CCGAGGGCCG AGGAGTATGA GTTCCTGACA 
CCCATGGAGG AGGCTCCCAA AGGCATGCTT GCTCGGGGCA GTTACAACAT CAAGTCGCGC 
TTCACAGATG ACGACAAGAC TGACCACCTG TCCTGGGAGT GGAATCTCAC CATCAAAAAG 
GAGTGGAAGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598557.3
CDS	122..736
Translation	MAEQEPTAEQLAQIAAENEEDEHSVNYKPPAQKSIQEIQELDKDDESLRKYKEALLGRVAVSADPNVPNVIVTRLTLVCSTAPGPLELDLTGDLESFKKQSFVLKEGVEYRIKISFRVNREIVSGMKYIQHTYRKGVKIDKTDYMVGSYGPRAEEYEFLTPMEEAPKGMLARGSYNIKSRFTDDDKTDHLSWEWNLTIKKEWKD

Target ORF information:

RefSeq Version	NM_133796.7
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GDP dissociation inhibitor (GDI) alpha (Arhgdia), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133796.7

ORF Insert Sequence:

ATGGCAGAAC AGGAACCCAC TGCTGAGCAG CTTGCGCAGA TAGCTGCAGA GAATGAGGAA 
GATGAGCACT CTGTGAACTA CAAGCCTCCA GCCCAGAAGA GCATCCAGGA GATCCAGGAA 
CTGGACAAGG ACGATGAAAG CCTCCGAAAG TACAAGGAGG CCCTGCTGGG CCGAGTAGCT 
GTCTCTGCAG ACCCCAACGT CCCCAATGTC ATTGTGACTC GACTGACCTT GGTATGCAGC 
ACTGCCCCAG GCCCTCTGGA ACTGGACCTG ACAGGTGATC TGGAGAGCTT CAAGAAACAG 
TCGTTTGTCT TGAAGGAAGG TGTGGAGTAC CGGATAAAAA TCTCTTTCCG GGTGAACAGA 
GAGATCGTGT CCGGCATGAA GTACATCCAA CACACATACA GGAAAGGGGT CAAAATTGAC 
AAGACTGACT ACATGGTCGG GAGCTATGGG CCGAGGGCCG AGGAGTATGA GTTCCTGACA 
CCCATGGAGG AGGCTCCCAA AGGCATGCTT GCTCGGGGCA GTTACAACAT CAAGTCGCGC 
TTCACAGATG ACGACAAGAC TGACCACCTG TCCTGGGAGT GGAATCTCAC CATCAAAAAG 
GAGTGGAAGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgdia ( NM_133796.8 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598557.3 Mus musculus Rho GDP dissociation inhibitor (GDI) alpha (Arhgdia), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu16256
Clone ID Related Accession (Same CDS sequence)	XM_006532549.3 , NM_133796.7 , NM_133796.8 , XM_006532549.2 , NM_001363425.1
Accession Version	NM_133796.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	rho GDP-dissociation inhibitor 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL663030.12, CJ091996.1 and AK165241.1. On May 18, 2018 this sequence version replaced NM_133796.7. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK170718.1, AK165241.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGAAC AGGAACCCAC TGCTGAGCAG CTTGCGCAGA TAGCTGCAGA GAATGAGGAA 
GATGAGCACT CTGTGAACTA CAAGCCTCCA GCCCAGAAGA GCATCCAGGA GATCCAGGAA 
CTGGACAAGG ACGATGAAAG CCTCCGAAAG TACAAGGAGG CCCTGCTGGG CCGAGTAGCT 
GTCTCTGCAG ACCCCAACGT CCCCAATGTC ATTGTGACTC GACTGACCTT GGTATGCAGC 
ACTGCCCCAG GCCCTCTGGA ACTGGACCTG ACAGGTGATC TGGAGAGCTT CAAGAAACAG 
TCGTTTGTCT TGAAGGAAGG TGTGGAGTAC CGGATAAAAA TCTCTTTCCG GGTGAACAGA 
GAGATCGTGT CCGGCATGAA GTACATCCAA CACACATACA GGAAAGGGGT CAAAATTGAC 
AAGACTGACT ACATGGTCGG GAGCTATGGG CCGAGGGCCG AGGAGTATGA GTTCCTGACA 
CCCATGGAGG AGGCTCCCAA AGGCATGCTT GCTCGGGGCA GTTACAACAT CAAGTCGCGC 
TTCACAGATG ACGACAAGAC TGACCACCTG TCCTGGGAGT GGAATCTCAC CATCAAAAAG 
GAGTGGAAGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598557.3
CDS	126..740
Translation	MAEQEPTAEQLAQIAAENEEDEHSVNYKPPAQKSIQEIQELDKDDESLRKYKEALLGRVAVSADPNVPNVIVTRLTLVCSTAPGPLELDLTGDLESFKKQSFVLKEGVEYRIKISFRVNREIVSGMKYIQHTYRKGVKIDKTDYMVGSYGPRAEEYEFLTPMEEAPKGMLARGSYNIKSRFTDDDKTDHLSWEWNLTIKKEWKD

Target ORF information:

RefSeq Version	NM_133796.8
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GDP dissociation inhibitor (GDI) alpha (Arhgdia), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133796.8

ORF Insert Sequence:

ATGGCAGAAC AGGAACCCAC TGCTGAGCAG CTTGCGCAGA TAGCTGCAGA GAATGAGGAA 
GATGAGCACT CTGTGAACTA CAAGCCTCCA GCCCAGAAGA GCATCCAGGA GATCCAGGAA 
CTGGACAAGG ACGATGAAAG CCTCCGAAAG TACAAGGAGG CCCTGCTGGG CCGAGTAGCT 
GTCTCTGCAG ACCCCAACGT CCCCAATGTC ATTGTGACTC GACTGACCTT GGTATGCAGC 
ACTGCCCCAG GCCCTCTGGA ACTGGACCTG ACAGGTGATC TGGAGAGCTT CAAGAAACAG 
TCGTTTGTCT TGAAGGAAGG TGTGGAGTAC CGGATAAAAA TCTCTTTCCG GGTGAACAGA 
GAGATCGTGT CCGGCATGAA GTACATCCAA CACACATACA GGAAAGGGGT CAAAATTGAC 
AAGACTGACT ACATGGTCGG GAGCTATGGG CCGAGGGCCG AGGAGTATGA GTTCCTGACA 
CCCATGGAGG AGGCTCCCAA AGGCATGCTT GCTCGGGGCA GTTACAACAT CAAGTCGCGC 
TTCACAGATG ACGACAAGAC TGACCACCTG TCCTGGGAGT GGAATCTCAC CATCAAAAAG 
GAGTGGAAGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgdia ( NM_001363425.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001350354.1 Mus musculus Rho GDP dissociation inhibitor (GDI) alpha (Arhgdia), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu16256
Clone ID Related Accession (Same CDS sequence)	XM_006532549.3 , NM_133796.7 , NM_133796.8 , XM_006532549.2 , NM_001363425.1
Accession Version	NM_001363425.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-23
Organism	Mus musculus(house mouse)
Product	rho GDP-dissociation inhibitor 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK077343.1 and AL663030.12. On May 18, 2018 this sequence version replaced XM_006532549.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK077343.1, SRR1660819.117791.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGAAC AGGAACCCAC TGCTGAGCAG CTTGCGCAGA TAGCTGCAGA GAATGAGGAA 
GATGAGCACT CTGTGAACTA CAAGCCTCCA GCCCAGAAGA GCATCCAGGA GATCCAGGAA 
CTGGACAAGG ACGATGAAAG CCTCCGAAAG TACAAGGAGG CCCTGCTGGG CCGAGTAGCT 
GTCTCTGCAG ACCCCAACGT CCCCAATGTC ATTGTGACTC GACTGACCTT GGTATGCAGC 
ACTGCCCCAG GCCCTCTGGA ACTGGACCTG ACAGGTGATC TGGAGAGCTT CAAGAAACAG 
TCGTTTGTCT TGAAGGAAGG TGTGGAGTAC CGGATAAAAA TCTCTTTCCG GGTGAACAGA 
GAGATCGTGT CCGGCATGAA GTACATCCAA CACACATACA GGAAAGGGGT CAAAATTGAC 
AAGACTGACT ACATGGTCGG GAGCTATGGG CCGAGGGCCG AGGAGTATGA GTTCCTGACA 
CCCATGGAGG AGGCTCCCAA AGGCATGCTT GCTCGGGGCA GTTACAACAT CAAGTCGCGC 
TTCACAGATG ACGACAAGAC TGACCACCTG TCCTGGGAGT GGAATCTCAC CATCAAAAAG 
GAGTGGAAGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350354.1
CDS	281..895
Translation	MAEQEPTAEQLAQIAAENEEDEHSVNYKPPAQKSIQEIQELDKDDESLRKYKEALLGRVAVSADPNVPNVIVTRLTLVCSTAPGPLELDLTGDLESFKKQSFVLKEGVEYRIKISFRVNREIVSGMKYIQHTYRKGVKIDKTDYMVGSYGPRAEEYEFLTPMEEAPKGMLARGSYNIKSRFTDDDKTDHLSWEWNLTIKKEWKD

Target ORF information:

RefSeq Version	NM_001363425.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GDP dissociation inhibitor (GDI) alpha (Arhgdia), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363425.1

ORF Insert Sequence:

ATGGCAGAAC AGGAACCCAC TGCTGAGCAG CTTGCGCAGA TAGCTGCAGA GAATGAGGAA 
GATGAGCACT CTGTGAACTA CAAGCCTCCA GCCCAGAAGA GCATCCAGGA GATCCAGGAA 
CTGGACAAGG ACGATGAAAG CCTCCGAAAG TACAAGGAGG CCCTGCTGGG CCGAGTAGCT 
GTCTCTGCAG ACCCCAACGT CCCCAATGTC ATTGTGACTC GACTGACCTT GGTATGCAGC 
ACTGCCCCAG GCCCTCTGGA ACTGGACCTG ACAGGTGATC TGGAGAGCTT CAAGAAACAG 
TCGTTTGTCT TGAAGGAAGG TGTGGAGTAC CGGATAAAAA TCTCTTTCCG GGTGAACAGA 
GAGATCGTGT CCGGCATGAA GTACATCCAA CACACATACA GGAAAGGGGT CAAAATTGAC 
AAGACTGACT ACATGGTCGG GAGCTATGGG CCGAGGGCCG AGGAGTATGA GTTCCTGACA 
CCCATGGAGG AGGCTCCCAA AGGCATGCTT GCTCGGGGCA GTTACAACAT CAAGTCGCGC 
TTCACAGATG ACGACAAGAC TGACCACCTG TCCTGGGAGT GGAATCTCAC CATCAAAAAG 
GAGTGGAAGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgdia ( XM_006532549.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532612.1 Mus musculus Rho GDP dissociation inhibitor (GDI) alpha (Arhgdia), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu16256
Clone ID Related Accession (Same CDS sequence)	XM_006532549.3 , NM_133796.7 , NM_133796.8 , XM_006532549.2 , NM_001363425.1
Accession Version	XM_006532549.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rho GDP-dissociation inhibitor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532549.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAC AGGAACCCAC TGCTGAGCAG CTTGCGCAGA TAGCTGCAGA GAATGAGGAA 
GATGAGCACT CTGTGAACTA CAAGCCTCCA GCCCAGAAGA GCATCCAGGA GATCCAGGAA 
CTGGACAAGG ACGATGAAAG CCTCCGAAAG TACAAGGAGG CCCTGCTGGG CCGAGTAGCT 
GTCTCTGCAG ACCCCAACGT CCCCAATGTC ATTGTGACTC GACTGACCTT GGTATGCAGC 
ACTGCCCCAG GCCCTCTGGA ACTGGACCTG ACAGGTGATC TGGAGAGCTT CAAGAAACAG 
TCGTTTGTCT TGAAGGAAGG TGTGGAGTAC CGGATAAAAA TCTCTTTCCG GGTGAACAGA 
GAGATCGTGT CCGGCATGAA GTACATCCAA CACACATACA GGAAAGGGGT CAAAATTGAC 
AAGACTGACT ACATGGTCGG GAGCTATGGG CCGAGGGCCG AGGAGTATGA GTTCCTGACA 
CCCATGGAGG AGGCTCCCAA AGGCATGCTT GCTCGGGGCA GTTACAACAT CAAGTCGCGC 
TTCACAGATG ACGACAAGAC TGACCACCTG TCCTGGGAGT GGAATCTCAC CATCAAAAAG 
GAGTGGAAGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532612.1
CDS	276..890
Translation	MAEQEPTAEQLAQIAAENEEDEHSVNYKPPAQKSIQEIQELDKDDESLRKYKEALLGRVAVSADPNVPNVIVTRLTLVCSTAPGPLELDLTGDLESFKKQSFVLKEGVEYRIKISFRVNREIVSGMKYIQHTYRKGVKIDKTDYMVGSYGPRAEEYEFLTPMEEAPKGMLARGSYNIKSRFTDDDKTDHLSWEWNLTIKKEWKD

Target ORF information:

RefSeq Version	XM_006532549.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GDP dissociation inhibitor (GDI) alpha (Arhgdia), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532549.3

ORF Insert Sequence:

ATGGCAGAAC AGGAACCCAC TGCTGAGCAG CTTGCGCAGA TAGCTGCAGA GAATGAGGAA 
GATGAGCACT CTGTGAACTA CAAGCCTCCA GCCCAGAAGA GCATCCAGGA GATCCAGGAA 
CTGGACAAGG ACGATGAAAG CCTCCGAAAG TACAAGGAGG CCCTGCTGGG CCGAGTAGCT 
GTCTCTGCAG ACCCCAACGT CCCCAATGTC ATTGTGACTC GACTGACCTT GGTATGCAGC 
ACTGCCCCAG GCCCTCTGGA ACTGGACCTG ACAGGTGATC TGGAGAGCTT CAAGAAACAG 
TCGTTTGTCT TGAAGGAAGG TGTGGAGTAC CGGATAAAAA TCTCTTTCCG GGTGAACAGA 
GAGATCGTGT CCGGCATGAA GTACATCCAA CACACATACA GGAAAGGGGT CAAAATTGAC 
AAGACTGACT ACATGGTCGG GAGCTATGGG CCGAGGGCCG AGGAGTATGA GTTCCTGACA 
CCCATGGAGG AGGCTCCCAA AGGCATGCTT GCTCGGGGCA GTTACAACAT CAAGTCGCGC 
TTCACAGATG ACGACAAGAC TGACCACCTG TCCTGGGAGT GGAATCTCAC CATCAAAAAG 
GAGTGGAAGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgdia ( XM_006532549.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532612.1 Mus musculus Rho GDP dissociation inhibitor (GDI) alpha (Arhgdia), transcript variant X1, mRNA.

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CloneID	OMu16256
Clone ID Related Accession (Same CDS sequence)	XM_006532549.3 , NM_133796.7 , NM_133796.8 , XM_006532549.2 , NM_001363425.1
Accession Version	XM_006532549.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	rho GDP-dissociation inhibitor 1 isoform X1
Comment

ATGGCAGAAC AGGAACCCAC TGCTGAGCAG CTTGCGCAGA TAGCTGCAGA GAATGAGGAA 
GATGAGCACT CTGTGAACTA CAAGCCTCCA GCCCAGAAGA GCATCCAGGA GATCCAGGAA 
CTGGACAAGG ACGATGAAAG CCTCCGAAAG TACAAGGAGG CCCTGCTGGG CCGAGTAGCT 
GTCTCTGCAG ACCCCAACGT CCCCAATGTC ATTGTGACTC GACTGACCTT GGTATGCAGC 
ACTGCCCCAG GCCCTCTGGA ACTGGACCTG ACAGGTGATC TGGAGAGCTT CAAGAAACAG 
TCGTTTGTCT TGAAGGAAGG TGTGGAGTAC CGGATAAAAA TCTCTTTCCG GGTGAACAGA 
GAGATCGTGT CCGGCATGAA GTACATCCAA CACACATACA GGAAAGGGGT CAAAATTGAC 
AAGACTGACT ACATGGTCGG GAGCTATGGG CCGAGGGCCG AGGAGTATGA GTTCCTGACA 
CCCATGGAGG AGGCTCCCAA AGGCATGCTT GCTCGGGGCA GTTACAACAT CAAGTCGCGC 
TTCACAGATG ACGACAAGAC TGACCACCTG TCCTGGGAGT GGAATCTCAC CATCAAAAAG 
GAGTGGAAGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532612.1
Translation	MAEQEPTAEQLAQIAAENEEDEHSVNYKPPAQKSIQEIQELDKDDESLRKYKEALLGRVAVSADPNVPNVIVTRLTLVCSTAPGPLELDLTGDLESFKKQSFVLKEGVEYRIKISFRVNREIVSGMKYIQHTYRKGVKIDKTDYMVGSYGPRAEEYEFLTPMEEAPKGMLARGSYNIKSRFTDDDKTDHLSWEWNLTIKKEWKD

Target ORF information:

RefSeq Version	XM_006532549.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus Rho GDP dissociation inhibitor (GDI) alpha (Arhgdia), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532549.2

ORF Insert Sequence:

ATGGCAGAAC AGGAACCCAC TGCTGAGCAG CTTGCGCAGA TAGCTGCAGA GAATGAGGAA 
GATGAGCACT CTGTGAACTA CAAGCCTCCA GCCCAGAAGA GCATCCAGGA GATCCAGGAA 
CTGGACAAGG ACGATGAAAG CCTCCGAAAG TACAAGGAGG CCCTGCTGGG CCGAGTAGCT 
GTCTCTGCAG ACCCCAACGT CCCCAATGTC ATTGTGACTC GACTGACCTT GGTATGCAGC 
ACTGCCCCAG GCCCTCTGGA ACTGGACCTG ACAGGTGATC TGGAGAGCTT CAAGAAACAG 
TCGTTTGTCT TGAAGGAAGG TGTGGAGTAC CGGATAAAAA TCTCTTTCCG GGTGAACAGA 
GAGATCGTGT CCGGCATGAA GTACATCCAA CACACATACA GGAAAGGGGT CAAAATTGAC 
AAGACTGACT ACATGGTCGG GAGCTATGGG CCGAGGGCCG AGGAGTATGA GTTCCTGACA 
CCCATGGAGG AGGCTCCCAA AGGCATGCTT GCTCGGGGCA GTTACAACAT CAAGTCGCGC 
TTCACAGATG ACGACAAGAC TGACCACCTG TCCTGGGAGT GGAATCTCAC CATCAAAAAG 
GAGTGGAAGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Impaired ?GDI Function in the X-Linked Intellectual Disability: The Impact on Astroglia Vesicle Dynamics.
Molecular neurobiology54(4)2458-2468(2017 05)
Potokar M,Jorga?evski J,Lacovich V,Kreft M,Vardjan N,Bianchi V,D'Adamo P,Zorec R

Disease-causing mutations of RhoGDI? induce Rac1 hyperactivation in podocytes.
Small GTPases7(2)107-21(2016 Apr)
Auguste D,Maier M,Baldwin C,Aoudjit L,Robins R,Gupta IR,Takano T

Protein tyrosine phosphatase-PEST and ?8 integrin regulate spatiotemporal patterns of RhoGDI1 activation in migrating cells.
Molecular and cellular biology35(8)1401-13(2015 Apr)
Lee HS,Cheerathodi M,Chaki SP,Reyes SB,Zheng Y,Lu Z,Paidassi H,DerMardirossian C,Lacy-Hulbert A,Rivera GM,McCarty JH

Role of Rho GDP dissociation inhibitor ? in control of epithelial sodium channel (ENaC)-mediated sodium reabsorption.
The Journal of biological chemistry289(41)28651-9(2014 Oct)
Pavlov TS,Levchenko V,Staruschenko A

Coronin-1C and RCC2 guide mesenchymal migration by trafficking Rac1 and controlling GEF exposure.
Journal of cell science127(Pt 19)4292-307(2014 Oct)
Williamson RC,Cowell CA,Hammond CL,Bergen DJ,Roper JA,Feng Y,Rendall TC,Race PR,Bass MD

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Arhgdia cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム