Prm1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prm1
Entrez Gene ID	19118
Full Name	protamine 1
Synonyms	Prm-1
General protein information	Preferred Names protamine 1 Names sperm protamine P1 cysteine-rich protamine
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16 A1\|16 5.87 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_013637.4	NP_038665.1	sperm protamine P1
NM_013637.5	NP_038665.1	sperm protamine P1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The Antagonistic Gene Paralogs Upf3a and Upf3b Govern Nonsense-Mediated RNA Decay.
Shum EY, Jones SH, Shao A, Dumdie J, Krause MD, Chan WK, Lou CH, Espinoza JL, Song HW, Phan MH, Ramaiah M, Huang L, McCarrey JR, Peterson KJ, De Rooij DG, Cook-Andersen H, Wilkinson MF
Cell165(2)382-95(2016 Apr)

JMJD1C Exhibits Multiple Functions in Epigenetic Regulation during Spermatogenesis.
Nakajima R, Okano H, Noce T
PloS one11(9)e0163466(2016)

FANCB is essential in the male germline and regulates H3K9 methylation on the sex chromosomes during meiosis.
Kato Y, Alavattam KG, Sin HS, Meetei AR, Pang Q, Andreassen PR, Namekawa SH
Human molecular genetics24(18)5234-49(2015 Sep)

The RNA helicase MOV10L1 binds piRNA precursors to initiate piRNA processing.
Vourekas A, Zheng K, Fu Q, Maragkakis M, Alexiou P, Ma J, Pillai RS, Mourelatos Z, Wang PJ
Genes & development29(6)617-29(2015 Mar)

Controlling fertilization and cAMP signaling in sperm by optogenetics.
Jansen V, Alvarez L, Balbach M, Str?nker T, Hegemann P, Kaupp UB, Wachten D
eLife4(2015 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that male mice from lines showing moderate transgene expression were fully fertile whereas strong overexpression of the Prm1-EGFP fusion protein resulted in complete and dominant male sterility.
Title: Genetic vasectomy-overexpression of Prm1-EGFP fusion protein in elongating spermatids causes dominant male sterility in mice.

Study shows that protamine 1 and 2 mRNAs, which are specific to the round spermatid stage, are repressed at the two-pronuclei and two-cell stages postfertilization, respectively, in embryos created by round spermatid injection, by distinct mechanisms.
Title: Transcriptional and epigenetic status of protamine 1 and 2 genes following round spermatids injection into mouse oocytes.

JHDM2A directly binds to and controls the expression of transition nuclear protein 1 (Tnp1) and protamine 1 (Prm1) genes, the products of which are required for packaging and condensation of sperm chromatin
Title: Histone demethylase JHDM2A is critical for Tnp1 and Prm1 transcription and spermatogenesis.

Germ cell nuclear factor acts as a repressor of basal transcription on both the Protamine 1 and Protamine 2 promoters.
Title: Reciprocal regulation of the mouse protamine genes by the orphan nuclear receptor germ cell nuclear factor and CREMtau.

docking of protamine 1 to the nuclear envelope is an important intermediate step in spermiogenesis
Title: Temporal association of protamine 1 with the inner nuclear membrane protein lamin B receptor during spermiogenesis.

The following Prm1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prm1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu09724	NM_013637.5 Latest version!	Mus musculus protamine 1 (Prm1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
View Old Accession Versions >>
OMu09724	NM_013637.4	Mus musculus protamine 1 (Prm1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prm1 ( NM_013637.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038665.1 Mus musculus protamine 1 (Prm1), mRNA.

Price & Availability↑

CloneID	OMu09724
Clone ID Related Accession (Same CDS sequence)	NM_013637.4 , NM_013637.5
Accession Version	NM_013637.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-28
Organism	Mus musculus(house mouse)
Product	sperm protamine P1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK007068.1. On Jul 27, 2004 this sequence version replaced NM_013637.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X14003.1, AK007068.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121

ATGGCCAGAT ACCGATGCTG CCGCAGCAAA AGCAGGAGCA GATGCCGCCG TCGCAGGCGA 
AGATGTCGCA GACGGAGGAG GCGATGCTGC CGGCGGAGGA GGCGAAGATG CTGCCGTCGC 
CGCCGCTCAT ACACCATAAG GTGTAAAAAA TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038665.1
CDS	94..249
Translation	MARYRCCRSKSRSRCRRRRRRCRRRRRRCCRRRRRRCCRRRRSYTIRCKKY

Target ORF information:

RefSeq Version	NM_013637.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus protamine 1 (Prm1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013637.4

ORF Insert Sequence:

1
61
121

ATGGCCAGAT ACCGATGCTG CCGCAGCAAA AGCAGGAGCA GATGCCGCCG TCGCAGGCGA 
AGATGTCGCA GACGGAGGAG GCGATGCTGC CGGCGGAGGA GGCGAAGATG CTGCCGTCGC 
CGCCGCTCAT ACACCATAAG GTGTAAAAAA TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prm1 ( NM_013637.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038665.1 Mus musculus protamine 1 (Prm1), mRNA.

Price & Availability↑

CloneID	OMu09724
Clone ID Related Accession (Same CDS sequence)	NM_013637.4 , NM_013637.5
Accession Version	NM_013637.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-23
Organism	Mus musculus(house mouse)
Product	sperm protamine P1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT010583.8. On Aug 14, 2018 this sequence version replaced NM_013637.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CN842143.1, BU937421.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121

ATGGCCAGAT ACCGATGCTG CCGCAGCAAA AGCAGGAGCA GATGCCGCCG TCGCAGGCGA 
AGATGTCGCA GACGGAGGAG GCGATGCTGC CGGCGGAGGA GGCGAAGATG CTGCCGTCGC 
CGCCGCTCAT ACACCATAAG GTGTAAAAAA TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038665.1
CDS	93..248
Translation	MARYRCCRSKSRSRCRRRRRRCRRRRRRCCRRRRRRCCRRRRSYTIRCKKY

Target ORF information:

RefSeq Version	NM_013637.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus protamine 1 (Prm1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013637.5

ORF Insert Sequence:

1
61
121

ATGGCCAGAT ACCGATGCTG CCGCAGCAAA AGCAGGAGCA GATGCCGCCG TCGCAGGCGA 
AGATGTCGCA GACGGAGGAG GCGATGCTGC CGGCGGAGGA GGCGAAGATG CTGCCGTCGC 
CGCCGCTCAT ACACCATAAG GTGTAAAAAA TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Antagonistic Gene Paralogs Upf3a and Upf3b Govern Nonsense-Mediated RNA Decay.
Cell165(2)382-95(2016 Apr)
Shum EY,Jones SH,Shao A,Dumdie J,Krause MD,Chan WK,Lou CH,Espinoza JL,Song HW,Phan MH,Ramaiah M,Huang L,McCarrey JR,Peterson KJ,De Rooij DG,Cook-Andersen H,Wilkinson MF

JMJD1C Exhibits Multiple Functions in Epigenetic Regulation during Spermatogenesis.
PloS one11(9)e0163466(2016)
Nakajima R,Okano H,Noce T

FANCB is essential in the male germline and regulates H3K9 methylation on the sex chromosomes during meiosis.
Human molecular genetics24(18)5234-49(2015 Sep)
Kato Y,Alavattam KG,Sin HS,Meetei AR,Pang Q,Andreassen PR,Namekawa SH

The RNA helicase MOV10L1 binds piRNA precursors to initiate piRNA processing.
Genes & development29(6)617-29(2015 Mar)
Vourekas A,Zheng K,Fu Q,Maragkakis M,Alexiou P,Ma J,Pillai RS,Mourelatos Z,Wang PJ

Controlling fertilization and cAMP signaling in sperm by optogenetics.
eLife4(2015 Jan)
Jansen V,Alvarez L,Balbach M,Str?nker T,Hegemann P,Kaupp UB,Wachten D

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Prm1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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