Plcd1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Plcd1
Entrez Gene ID	18799
Full Name	phospholipase C, delta 1
Synonyms	AW212592,C79986
General protein information	Preferred Names phospholipase C, delta 1 Names 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 PLC-delta 1 phosphoinositide phospholipase C-delta-1 phospholipase C delta 1 Lf
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 F3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006511984.3	XP_006512047.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 isoform X1
XM_006511984.2	XP_006512047.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 isoform X1
NM_019676.3	NP_062650.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 isoform 2
NM_001293648.1	NP_001280577.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 isoform 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Plcd1	NP_062650.1
Homo sapiens (human)	PLCD1	NP_001124436.1
	PLCD1	XP_001089399.1
Oryza sativa (rice)	Os05g0127200	NP_001054531.1
Pan troglodytes (chimpanzee)	PLCD1	XP_003309769.2
Rattus norvegicus (Norway rat)	Plcd1	NP_058731.1
Canis lupus familiaris (dog)	PLCD1	XP_859460.2
Xenopus tropicalis (tropical clawed frog)	plcd1	XP_004915652.1
Bos taurus (cattle)	PLCD1	NP_001075045.1
Gallus gallus (chicken)	PLCD1	XP_418522.2

Gene Ontology
Bibliography

Related articles in PubMed

Actomyosin polarisation through PLC-PKC triggers symmetry breaking of the mouse embryo.
Zhu M, Leung CY, Shahbazi MN, Zernicka-Goetz M
Nature communications8(1)921(2017 Oct)

Phospholipase C?1 regulates p38 MAPK activity and skin barrier integrity.
Kanemaru K, Nakamura Y, Totoki K, Fukuyama T, Shoji M, Kaneko H, Shiratori K, Yoneda A, Inoue T, Iwakura Y, Kabashima K, Fukami K
Cell death and differentiation24(6)1079-1090(2017 Jun)

Karyopherin Alpha 1 Regulates Satellite Cell Proliferation and Survival by Modulating Nuclear Import.
Choo HJ, Cutler A, Rother F, Bader M, Pavlath GK
Stem cells (Dayton, Ohio)34(11)2784-2797(2016 Nov)

[Phospholipase C ?1 (PLCD1) inhibits the proliferation, invasion and migration of CAPAN-1 and BXPC-3 pancreatic cancer cells].
Hu D, Jiang Z
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology32(6)739-45(2016 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that phospholipase C delta1 (PLCD1) transfection can induce the apoptosis of CAPAN-1 and BXPC-3 pancreatic cancer cells, arrest the cell cycle in G0 /G1 phase.
Title: [Phospholipase C ?1 (PLCD1) inhibits the proliferation, invasion and migration of CAPAN-1 and BXPC-3 pancreatic cancer cells].

PLCd1 negatively regulates lipopolysaccharide-induced production of IL-1b and Fc gamma receptor-mediated phagocytosis in macrophages.
Title: Phospholipase C ?1 in macrophages negatively regulates TLR4-induced proinflammatory cytokine production and Fc? receptor-mediated phagocytosis.

the Ca(2+) dependence of the interaction between PRIP-C2 and Syt1-C2A was attributed to Ca(2+) binding with Syt1-C2A, but not PRIP-C2, using a series of mutants prepared from both C2 domains.
Title: Hetero-oligomerization of C2 domains of phospholipase C-related but catalytically inactive protein and synaptotagmin-1.

Results indicate that PLCdelta1 and PLCdelta3 are required for cardiomyocyte survival and normal cardiac function.
Title: Simultaneous loss of phospholipase C?1 and phospholipase C?3 causes cardiomyocyte apoptosis and cardiomyopathy.

Expression of PLCdelta1 is increased in ALS mouse spinal cord and in neurons from ALS mice. Genetic ablation of this protein in ALS mice significantly increases survival
Title: Genetic ablation of phospholipase C delta 1 increases survival in SOD1(G93A) mice.

The following Plcd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Plcd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu23448	NM_019676.3 Latest version!	Mus musculus phospholipase C, delta 1 (Plcd1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu23450	NM_001293648.1 Latest version!	Mus musculus phospholipase C, delta 1 (Plcd1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu43330	XM_006511984.3 Latest version!	Mus musculus phospholipase C, delta 1 (Plcd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu43330	XM_006511984.2	Mus musculus phospholipase C, delta 1 (Plcd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Plcd1 ( NM_019676.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062650.1 Mus musculus phospholipase C, delta 1 (Plcd1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu23448
Clone ID Related Accession (Same CDS sequence)	NM_019676.3
Accession Version	NM_019676.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK028749.1 and BY383308.1. On Jun 12, 2014 this sequence version replaced NM_019676.2. Transcript Variant: This variant (2) differs in the 5' UTR and initiates translation at an alternate start codon, compared to variant 1. The encoded protein (isoform 2) has a distinct and shorter N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U85711.1, AK028749.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

ATGGACTCCG GTCGGGACTT CCTGACCTTG CACGGGCTGC AGGATGACCC GGACCTTCAG 
GCCCTCCTGA AGGGCAGCCA GCTTCTGAAG GTGAAGTCCA GCTCGTGGCG TAGAGAACGC 
TTCTACAAGC TACAGGAGGA CTGCAAGACC ATCTGGCAGG AATCTCGCAA GGTCATGAGG 
TCCCCGGAGT CGCAGCTGTT CTCCATCGAG GACATTCAGG AGGTGCGGAT GGGACACCGC 
ACAGAAGGCC TGGAGAAATT TGCCAGAGAC ATACCCGAGG ACCGATGCTT CTCCATCGTC 
TTCAAGGACC AGCGCAATAC CCTAGACCTC ATCGCCCCGT CTCCAGCTGA CGTCCAGCAT 
TGGGTGCAGG GCCTGCGCAA GATCATCGAC CGCTCGGGCT CCATGGACCA GCGGCAGAAG 
CTGCAGCACT GGATTCACTC CTGCTTGCGA AAGGCTGATA AAAACAAGGA TAACAAGATG 
AACTTCAAGG AGGTGAAGGA CTTCCTGAAG GAGCTCAACG TACAGGTGGA TGACAGCTAC 
GCCAGGAAGA TCTTCAGGGA ATGTGACCAC TCCCAGACAG ACTCGCTGGA GGATGAGGAG 
ATTGAGACCT TCTACAGGAT GCTGACCCAG CGGGCAGAGA TCGACCGTGC CTTTGCAGAG 
GCCGCAGGTT CTGCGGAGAC CCTGTCGGTG GAGAAGCTAG TGACGTTCCT GCAGCATCAG 
CAGCGGGAGG AGGAGGCGGG GCCAGCCTTG GCCCTCTCTC TCATTGAGCG CTACGAGCCC 
AGTGAGACTG CCAAAGCCCA GCGGCAGATG ACCAAGGATG GCTTCCTCAT GTACCTGCTG 
TCTGCTGACG GTAACGCCTT CAGCCTGGCG CACCGCCGTG TCTACCAGGA CATGAACCAG 
CCGCTCAGCC ACTACTTAGT GTCTTCTTCC CACAACACCT ACCTACTGGA GGACCAGCTC 
ACAGGGCCCA GCAGCACGGA GGCCTACATC CGGGCTCTGT GCAAAGGCTG CCGGTGTCTG 
GAGCTGGACT GCTGGGACGG CCCCAACCAG GAACCCATCA TCTACCATGG CTACACTTTT 
ACCTCTAAGA TACTTTTCTG CGACGTGCTC AGGGCCATCC GGGACTACGC CTTCAAGGCA 
TCCCCCTACC CAGTTATCCT GTCCCTGGAG AACCACTGCA GCCTGGAGCA ACAACGAGTC 
ATGGCTCATC ACCTGAGGGC CATCCTGGGG CCCATGCTGT TGGACCAGCC GCTGGATGGG 
GTTACCACGA GCCTGCCTTC GCCTGAGCAA CTGAAGGAAA AGATTCTGTT GAAAGGGAAG 
AAACTGGGCG GGCTGCTACC TGCTGGAGGG GAAAACGGGC CTGAAGCCAC CGATGTGTCT 
GACGAAGATG AGGCTGCTGA AATGGAAGAT GAGGCCGTGC GCAGCCAAGT GCAACACAAG 
CCCAAGGAGG ATAAGCTAAA GCTGGTGCCG GAGCTCTCCG ACATGGTCAT TTACTGCAAG 
AGTGTCCACT TTGGTGGCTT CTCCAGTCCT AGCACCTCTG GGCAGGCATT CTATGAGATG 
GCTTCCTTCT CCGAGAGCCG TGCCCTGCGG CTGCTGCAAG AATCAGGAAA TAGTTTTGTC 
CGCCATAACG TGGGCCATCT GAGCAGGATC TACCCAGCTG GGTGGAGGAC AGATTCCTCC 
AACTACAGTC CCGTGGAGAT GTGGAATGGA GGCTGCCAGA TTGTGGCTCT GAACTTCCAA 
ACCCCTGGGC CAGAAATGGA TGTTTACCTT GGCTGCTTCC AGGACAATGG AGGTTGTGGG 
TATGTGCTGA AACCGGCCTT CCTGAGAGAC CCTGACACCA CCTTCAACTC ACGGGCCCTG 
ACTCAGGGGC CCTGGTGGGC CCCGAAGAAG CTCCGAGTCT GGATCATCTC TGGACAGCAG 
CTGCCGAAGG TCAATAAGAA TAAGAATTCT ATCGTGGACC CCAAGGTGAT AGTGGAGATC 
CACGGTGTGG GCCAGGATGT GGCCAGCCGT CAGACAGCAG TTATTACCAA TAATGGTTTC 
AACCCACGGT GGGACACGGA GTTTGAGTTT GTGGTAGCAG TGCCTGACCT TGCCCTTGTA 
CGTTTCATGG TAGAGGATTA TGACTCCTCC TCTAAGAACG ACTTTATCGG CCAGAGTACT 
ATCCCCTGGA ACAGCCTCAA GCAGGGGTAC CGCCATGTCC ACCTCTTGTC TAAGAATGGA 
GACCTGCATC CATCAGCCAC TCTCTTTGTG AAGATCTCCA TCCAGGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062650.1
CDS	112..2382
Translation	MDSGRDFLTLHGLQDDPDLQALLKGSQLLKVKSSSWRRERFYKLQEDCKTIWQESRKVMRSPESQLFSIEDIQEVRMGHRTEGLEKFARDIPEDRCFSIVFKDQRNTLDLIAPSPADVQHWVQGLRKIIDRSGSMDQRQKLQHWIHSCLRKADKNKDNKMNFKEVKDFLKELNVQVDDSYARKIFRECDHSQTDSLEDEEIETFYRMLTQRAEIDRAFAEAAGSAETLSVEKLVTFLQHQQREEEAGPALALSLIERYEPSETAKAQRQMTKDGFLMYLLSADGNAFSLAHRRVYQDMNQPLSHYLVSSSHNTYLLEDQLTGPSSTEAYIRALCKGCRCLELDCWDGPNQEPIIYHGYTFTSKILFCDVLRAIRDYAFKASPYPVILSLENHCSLEQQRVMAHHLRAILGPMLLDQPLDGVTTSLPSPEQLKEKILLKGKKLGGLLPAGGENGPEATDVSDEDEAAEMEDEAVRSQVQHKPKEDKLKLVPELSDMVIYCKSVHFGGFSSPSTSGQAFYEMASFSESRALRLLQESGNSFVRHNVGHLSRIYPAGWRTDSSNYSPVEMWNGGCQIVALNFQTPGPEMDVYLGCFQDNGGCGYVLKPAFLRDPDTTFNSRALTQGPWWAPKKLRVWIISGQQLPKVNKNKNSIVDPKVIVEIHGVGQDVASRQTAVITNNGFNPRWDTEFEFVVAVPDLALVRFMVEDYDSSSKNDFIGQSTIPWNSLKQGYRHVHLLSKNGDLHPSATLFVKISIQD

Target ORF information:

RefSeq Version	NM_019676.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, delta 1 (Plcd1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019676.3

ORF Insert Sequence:

ATGGACTCCG GTCGGGACTT CCTGACCTTG CACGGGCTGC AGGATGACCC GGACCTTCAG 
GCCCTCCTGA AGGGCAGCCA GCTTCTGAAG GTGAAGTCCA GCTCGTGGCG TAGAGAACGC 
TTCTACAAGC TACAGGAGGA CTGCAAGACC ATCTGGCAGG AATCTCGCAA GGTCATGAGG 
TCCCCGGAGT CGCAGCTGTT CTCCATCGAG GACATTCAGG AGGTGCGGAT GGGACACCGC 
ACAGAAGGCC TGGAGAAATT TGCCAGAGAC ATACCCGAGG ACCGATGCTT CTCCATCGTC 
TTCAAGGACC AGCGCAATAC CCTAGACCTC ATCGCCCCGT CTCCAGCTGA CGTCCAGCAT 
TGGGTGCAGG GCCTGCGCAA GATCATCGAC CGCTCGGGCT CCATGGACCA GCGGCAGAAG 
CTGCAGCACT GGATTCACTC CTGCTTGCGA AAGGCTGATA AAAACAAGGA TAACAAGATG 
AACTTCAAGG AGGTGAAGGA CTTCCTGAAG GAGCTCAACG TACAGGTGGA TGACAGCTAC 
GCCAGGAAGA TCTTCAGGGA ATGTGACCAC TCCCAGACAG ACTCGCTGGA GGATGAGGAG 
ATTGAGACCT TCTACAGGAT GCTGACCCAG CGGGCAGAGA TCGACCGTGC CTTTGCAGAG 
GCCGCAGGTT CTGCGGAGAC CCTGTCGGTG GAGAAGCTAG TGACGTTCCT GCAGCATCAG 
CAGCGGGAGG AGGAGGCGGG GCCAGCCTTG GCCCTCTCTC TCATTGAGCG CTACGAGCCC 
AGTGAGACTG CCAAAGCCCA GCGGCAGATG ACCAAGGATG GCTTCCTCAT GTACCTGCTG 
TCTGCTGACG GTAACGCCTT CAGCCTGGCG CACCGCCGTG TCTACCAGGA CATGAACCAG 
CCGCTCAGCC ACTACTTAGT GTCTTCTTCC CACAACACCT ACCTACTGGA GGACCAGCTC 
ACAGGGCCCA GCAGCACGGA GGCCTACATC CGGGCTCTGT GCAAAGGCTG CCGGTGTCTG 
GAGCTGGACT GCTGGGACGG CCCCAACCAG GAACCCATCA TCTACCATGG CTACACTTTT 
ACCTCTAAGA TACTTTTCTG CGACGTGCTC AGGGCCATCC GGGACTACGC CTTCAAGGCA 
TCCCCCTACC CAGTTATCCT GTCCCTGGAG AACCACTGCA GCCTGGAGCA ACAACGAGTC 
ATGGCTCATC ACCTGAGGGC CATCCTGGGG CCCATGCTGT TGGACCAGCC GCTGGATGGG 
GTTACCACGA GCCTGCCTTC GCCTGAGCAA CTGAAGGAAA AGATTCTGTT GAAAGGGAAG 
AAACTGGGCG GGCTGCTACC TGCTGGAGGG GAAAACGGGC CTGAAGCCAC CGATGTGTCT 
GACGAAGATG AGGCTGCTGA AATGGAAGAT GAGGCCGTGC GCAGCCAAGT GCAACACAAG 
CCCAAGGAGG ATAAGCTAAA GCTGGTGCCG GAGCTCTCCG ACATGGTCAT TTACTGCAAG 
AGTGTCCACT TTGGTGGCTT CTCCAGTCCT AGCACCTCTG GGCAGGCATT CTATGAGATG 
GCTTCCTTCT CCGAGAGCCG TGCCCTGCGG CTGCTGCAAG AATCAGGAAA TAGTTTTGTC 
CGCCATAACG TGGGCCATCT GAGCAGGATC TACCCAGCTG GGTGGAGGAC AGATTCCTCC 
AACTACAGTC CCGTGGAGAT GTGGAATGGA GGCTGCCAGA TTGTGGCTCT GAACTTCCAA 
ACCCCTGGGC CAGAAATGGA TGTTTACCTT GGCTGCTTCC AGGACAATGG AGGTTGTGGG 
TATGTGCTGA AACCGGCCTT CCTGAGAGAC CCTGACACCA CCTTCAACTC ACGGGCCCTG 
ACTCAGGGGC CCTGGTGGGC CCCGAAGAAG CTCCGAGTCT GGATCATCTC TGGACAGCAG 
CTGCCGAAGG TCAATAAGAA TAAGAATTCT ATCGTGGACC CCAAGGTGAT AGTGGAGATC 
CACGGTGTGG GCCAGGATGT GGCCAGCCGT CAGACAGCAG TTATTACCAA TAATGGTTTC 
AACCCACGGT GGGACACGGA GTTTGAGTTT GTGGTAGCAG TGCCTGACCT TGCCCTTGTA 
CGTTTCATGG TAGAGGATTA TGACTCCTCC TCTAAGAACG ACTTTATCGG CCAGAGTACT 
ATCCCCTGGA ACAGCCTCAA GCAGGGGTAC CGCCATGTCC ACCTCTTGTC TAAGAATGGA 
GACCTGCATC CATCAGCCAC TCTCTTTGTG AAGATCTCCA TCCAGGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plcd1 ( NM_001293648.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001280577.1 Mus musculus phospholipase C, delta 1 (Plcd1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu23450
Clone ID Related Accession (Same CDS sequence)	NM_001293648.1
Accession Version	NM_001293648.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC055818.9, BB839358.1, AK028749.1 and BY383308.1. On Jun 12, 2014 this sequence version replaced XM_006511983.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: JN635596.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

ATGCAGTGTC TAGGGGTCCG GAGTTGCAGA CGGGGCAGCG TGAACCGCTC CCGGGAGCTC 
TACCTGCAGG AGCAGAGTCG CAAAGTAGCG GCGCTCAATG GACAGAGACT GGGGCTGCAG 
GATGACCCGG ACCTTCAGGC CCTCCTGAAG GGCAGCCAGC TTCTGAAGGT GAAGTCCAGC 
TCGTGGCGTA GAGAACGCTT CTACAAGCTA CAGGAGGACT GCAAGACCAT CTGGCAGGAA 
TCTCGCAAGG TCATGAGGTC CCCGGAGTCG CAGCTGTTCT CCATCGAGGA CATTCAGGAG 
GTGCGGATGG GACACCGCAC AGAAGGCCTG GAGAAATTTG CCAGAGACAT ACCCGAGGAC 
CGATGCTTCT CCATCGTCTT CAAGGACCAG CGCAATACCC TAGACCTCAT CGCCCCGTCT 
CCAGCTGACG TCCAGCATTG GGTGCAGGGC CTGCGCAAGA TCATCGACCG CTCGGGCTCC 
ATGGACCAGC GGCAGAAGCT GCAGCACTGG ATTCACTCCT GCTTGCGAAA GGCTGATAAA 
AACAAGGATA ACAAGATGAA CTTCAAGGAG GTGAAGGACT TCCTGAAGGA GCTCAACGTA 
CAGGTGGATG ACAGCTACGC CAGGAAGATC TTCAGGGAAT GTGACCACTC CCAGACAGAC 
TCGCTGGAGG ATGAGGAGAT TGAGACCTTC TACAGGATGC TGACCCAGCG GGCAGAGATC 
GACCGTGCCT TTGCAGAGGC CGCAGGTTCT GCGGAGACCC TGTCGGTGGA GAAGCTAGTG 
ACGTTCCTGC AGCATCAGCA GCGGGAGGAG GAGGCGGGGC CAGCCTTGGC CCTCTCTCTC 
ATTGAGCGCT ACGAGCCCAG TGAGACTGCC AAAGCCCAGC GGCAGATGAC CAAGGATGGC 
TTCCTCATGT ACCTGCTGTC TGCTGACGGT AACGCCTTCA GCCTGGCGCA CCGCCGTGTC 
TACCAGGACA TGAACCAGCC GCTCAGCCAC TACTTAGTGT CTTCTTCCCA CAACACCTAC 
CTACTGGAGG ACCAGCTCAC AGGGCCCAGC AGCACGGAGG CCTACATCCG GGCTCTGTGC 
AAAGGCTGCC GGTGTCTGGA GCTGGACTGC TGGGACGGCC CCAACCAGGA ACCCATCATC 
TACCATGGCT ACACTTTTAC CTCTAAGATA CTTTTCTGCG ACGTGCTCAG GGCCATCCGG 
GACTACGCCT TCAAGGCATC CCCCTACCCA GTTATCCTGT CCCTGGAGAA CCACTGCAGC 
CTGGAGCAAC AACGAGTCAT GGCTCATCAC CTGAGGGCCA TCCTGGGGCC CATGCTGTTG 
GACCAGCCGC TGGATGGGGT TACCACGAGC CTGCCTTCGC CTGAGCAACT GAAGGAAAAG 
ATTCTGTTGA AAGGGAAGAA ACTGGGCGGG CTGCTACCTG CTGGAGGGGA AAACGGGCCT 
GAAGCCACCG ATGTGTCTGA CGAAGATGAG GCTGCTGAAA TGGAAGATGA GGCCGTGCGC 
AGCCAAGTGC AACACAAGCC CAAGGAGGAT AAGCTAAAGC TGGTGCCGGA GCTCTCCGAC 
ATGGTCATTT ACTGCAAGAG TGTCCACTTT GGTGGCTTCT CCAGTCCTAG CACCTCTGGG 
CAGGCATTCT ATGAGATGGC TTCCTTCTCC GAGAGCCGTG CCCTGCGGCT GCTGCAAGAA 
TCAGGAAATA GTTTTGTCCG CCATAACGTG GGCCATCTGA GCAGGATCTA CCCAGCTGGG 
TGGAGGACAG ATTCCTCCAA CTACAGTCCC GTGGAGATGT GGAATGGAGG CTGCCAGATT 
GTGGCTCTGA ACTTCCAAAC CCCTGGGCCA GAAATGGATG TTTACCTTGG CTGCTTCCAG 
GACAATGGAG GTTGTGGGTA TGTGCTGAAA CCGGCCTTCC TGAGAGACCC TGACACCACC 
TTCAACTCAC GGGCCCTGAC TCAGGGGCCC TGGTGGGCCC CGAAGAAGCT CCGAGTCTGG 
ATCATCTCTG GACAGCAGCT GCCGAAGGTC AATAAGAATA AGAATTCTAT CGTGGACCCC 
AAGGTGATAG TGGAGATCCA CGGTGTGGGC CAGGATGTGG CCAGCCGTCA GACAGCAGTT 
ATTACCAATA ATGGTTTCAA CCCACGGTGG GACACGGAGT TTGAGTTTGT GGTAGCAGTG 
CCTGACCTTG CCCTTGTACG TTTCATGGTA GAGGATTATG ACTCCTCCTC TAAGAACGAC 
TTTATCGGCC AGAGTACTAT CCCCTGGAAC AGCCTCAAGC AGGGGTACCG CCATGTCCAC 
CTCTTGTCTA AGAATGGAGA CCTGCATCCA TCAGCCACTC TCTTTGTGAA GATCTCCATC 
CAGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001280577.1
CDS	83..2431
Translation	MQCLGVRSCRRGSVNRSRELYLQEQSRKVAALNGQRLGLQDDPDLQALLKGSQLLKVKSSSWRRERFYKLQEDCKTIWQESRKVMRSPESQLFSIEDIQEVRMGHRTEGLEKFARDIPEDRCFSIVFKDQRNTLDLIAPSPADVQHWVQGLRKIIDRSGSMDQRQKLQHWIHSCLRKADKNKDNKMNFKEVKDFLKELNVQVDDSYARKIFRECDHSQTDSLEDEEIETFYRMLTQRAEIDRAFAEAAGSAETLSVEKLVTFLQHQQREEEAGPALALSLIERYEPSETAKAQRQMTKDGFLMYLLSADGNAFSLAHRRVYQDMNQPLSHYLVSSSHNTYLLEDQLTGPSSTEAYIRALCKGCRCLELDCWDGPNQEPIIYHGYTFTSKILFCDVLRAIRDYAFKASPYPVILSLENHCSLEQQRVMAHHLRAILGPMLLDQPLDGVTTSLPSPEQLKEKILLKGKKLGGLLPAGGENGPEATDVSDEDEAAEMEDEAVRSQVQHKPKEDKLKLVPELSDMVIYCKSVHFGGFSSPSTSGQAFYEMASFSESRALRLLQESGNSFVRHNVGHLSRIYPAGWRTDSSNYSPVEMWNGGCQIVALNFQTPGPEMDVYLGCFQDNGGCGYVLKPAFLRDPDTTFNSRALTQGPWWAPKKLRVWIISGQQLPKVNKNKNSIVDPKVIVEIHGVGQDVASRQTAVITNNGFNPRWDTEFEFVVAVPDLALVRFMVEDYDSSSKNDFIGQSTIPWNSLKQGYRHVHLLSKNGDLHPSATLFVKISIQD

Target ORF information:

RefSeq Version	NM_001293648.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, delta 1 (Plcd1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001293648.1

ORF Insert Sequence:

ATGCAGTGTC TAGGGGTCCG GAGTTGCAGA CGGGGCAGCG TGAACCGCTC CCGGGAGCTC 
TACCTGCAGG AGCAGAGTCG CAAAGTAGCG GCGCTCAATG GACAGAGACT GGGGCTGCAG 
GATGACCCGG ACCTTCAGGC CCTCCTGAAG GGCAGCCAGC TTCTGAAGGT GAAGTCCAGC 
TCGTGGCGTA GAGAACGCTT CTACAAGCTA CAGGAGGACT GCAAGACCAT CTGGCAGGAA 
TCTCGCAAGG TCATGAGGTC CCCGGAGTCG CAGCTGTTCT CCATCGAGGA CATTCAGGAG 
GTGCGGATGG GACACCGCAC AGAAGGCCTG GAGAAATTTG CCAGAGACAT ACCCGAGGAC 
CGATGCTTCT CCATCGTCTT CAAGGACCAG CGCAATACCC TAGACCTCAT CGCCCCGTCT 
CCAGCTGACG TCCAGCATTG GGTGCAGGGC CTGCGCAAGA TCATCGACCG CTCGGGCTCC 
ATGGACCAGC GGCAGAAGCT GCAGCACTGG ATTCACTCCT GCTTGCGAAA GGCTGATAAA 
AACAAGGATA ACAAGATGAA CTTCAAGGAG GTGAAGGACT TCCTGAAGGA GCTCAACGTA 
CAGGTGGATG ACAGCTACGC CAGGAAGATC TTCAGGGAAT GTGACCACTC CCAGACAGAC 
TCGCTGGAGG ATGAGGAGAT TGAGACCTTC TACAGGATGC TGACCCAGCG GGCAGAGATC 
GACCGTGCCT TTGCAGAGGC CGCAGGTTCT GCGGAGACCC TGTCGGTGGA GAAGCTAGTG 
ACGTTCCTGC AGCATCAGCA GCGGGAGGAG GAGGCGGGGC CAGCCTTGGC CCTCTCTCTC 
ATTGAGCGCT ACGAGCCCAG TGAGACTGCC AAAGCCCAGC GGCAGATGAC CAAGGATGGC 
TTCCTCATGT ACCTGCTGTC TGCTGACGGT AACGCCTTCA GCCTGGCGCA CCGCCGTGTC 
TACCAGGACA TGAACCAGCC GCTCAGCCAC TACTTAGTGT CTTCTTCCCA CAACACCTAC 
CTACTGGAGG ACCAGCTCAC AGGGCCCAGC AGCACGGAGG CCTACATCCG GGCTCTGTGC 
AAAGGCTGCC GGTGTCTGGA GCTGGACTGC TGGGACGGCC CCAACCAGGA ACCCATCATC 
TACCATGGCT ACACTTTTAC CTCTAAGATA CTTTTCTGCG ACGTGCTCAG GGCCATCCGG 
GACTACGCCT TCAAGGCATC CCCCTACCCA GTTATCCTGT CCCTGGAGAA CCACTGCAGC 
CTGGAGCAAC AACGAGTCAT GGCTCATCAC CTGAGGGCCA TCCTGGGGCC CATGCTGTTG 
GACCAGCCGC TGGATGGGGT TACCACGAGC CTGCCTTCGC CTGAGCAACT GAAGGAAAAG 
ATTCTGTTGA AAGGGAAGAA ACTGGGCGGG CTGCTACCTG CTGGAGGGGA AAACGGGCCT 
GAAGCCACCG ATGTGTCTGA CGAAGATGAG GCTGCTGAAA TGGAAGATGA GGCCGTGCGC 
AGCCAAGTGC AACACAAGCC CAAGGAGGAT AAGCTAAAGC TGGTGCCGGA GCTCTCCGAC 
ATGGTCATTT ACTGCAAGAG TGTCCACTTT GGTGGCTTCT CCAGTCCTAG CACCTCTGGG 
CAGGCATTCT ATGAGATGGC TTCCTTCTCC GAGAGCCGTG CCCTGCGGCT GCTGCAAGAA 
TCAGGAAATA GTTTTGTCCG CCATAACGTG GGCCATCTGA GCAGGATCTA CCCAGCTGGG 
TGGAGGACAG ATTCCTCCAA CTACAGTCCC GTGGAGATGT GGAATGGAGG CTGCCAGATT 
GTGGCTCTGA ACTTCCAAAC CCCTGGGCCA GAAATGGATG TTTACCTTGG CTGCTTCCAG 
GACAATGGAG GTTGTGGGTA TGTGCTGAAA CCGGCCTTCC TGAGAGACCC TGACACCACC 
TTCAACTCAC GGGCCCTGAC TCAGGGGCCC TGGTGGGCCC CGAAGAAGCT CCGAGTCTGG 
ATCATCTCTG GACAGCAGCT GCCGAAGGTC AATAAGAATA AGAATTCTAT CGTGGACCCC 
AAGGTGATAG TGGAGATCCA CGGTGTGGGC CAGGATGTGG CCAGCCGTCA GACAGCAGTT 
ATTACCAATA ATGGTTTCAA CCCACGGTGG GACACGGAGT TTGAGTTTGT GGTAGCAGTG 
CCTGACCTTG CCCTTGTACG TTTCATGGTA GAGGATTATG ACTCCTCCTC TAAGAACGAC 
TTTATCGGCC AGAGTACTAT CCCCTGGAAC AGCCTCAAGC AGGGGTACCG CCATGTCCAC 
CTCTTGTCTA AGAATGGAGA CCTGCATCCA TCAGCCACTC TCTTTGTGAA GATCTCCATC 
CAGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plcd1 ( XM_006511984.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512047.1 Mus musculus phospholipase C, delta 1 (Plcd1), transcript variant X1, mRNA.

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CloneID	OMu43330
Clone ID Related Accession (Same CDS sequence)	XM_006511984.3 , XM_006511984.2
Accession Version	XM_006511984.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006511984.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTCCC CGGAGTCGCA GCTGTTCTCC ATCGAGGACA TTCAGGAGGT GCGGATGGGA 
CACCGCACAG AAGGCCTGGA GAAATTTGCC AGAGACATAC CCGAGGACCG ATGCTTCTCC 
ATCGTCTTCA AGGACCAGCG CAATACCCTA GACCTCATCG CCCCGTCTCC AGCTGACGTC 
CAGCATTGGG TGCAGGGCCT GCGCAAGATC ATCGACCGCT CGGGCTCCAT GGACCAGCGG 
CAGAAGCTGC AGCACTGGAT TCACTCCTGC TTGCGAAAGG CTGATAAAAA CAAGGATAAC 
AAGATGAACT TCAAGGAGGT GAAGGACTTC CTGAAGGAGC TCAACGTACA GGTGGATGAC 
AGCTACGCCA GGAAGATCTT CAGGGAATGT GACCACTCCC AGACAGACTC GCTGGAGGAT 
GAGGAGATTG AGACCTTCTA CAGGATGCTG ACCCAGCGGG CAGAGATCGA CCGTGCCTTT 
GCAGAGGCCG CAGGTTCTGC GGAGACCCTG TCGGTGGAGA AGCTAGTGAC GTTCCTGCAG 
CATCAGCAGC GGGAGGAGGA GGCGGGGCCA GCCTTGGCCC TCTCTCTCAT TGAGCGCTAC 
GAGCCCAGTG AGACTGCCAA AGCCCAGCGG CAGATGACCA AGGATGGCTT CCTCATGTAC 
CTGCTGTCTG CTGACGGTAA CGCCTTCAGC CTGGCGCACC GCCGTGTCTA CCAGGACATG 
AACCAGCCGC TCAGCCACTA CTTAGTGTCT TCTTCCCACA ACACCTACCT ACTGGAGGAC 
CAGCTCACAG GGCCCAGCAG CACGGAGGCC TACATCCGGG CTCTGTGCAA AGGCTGCCGG 
TGTCTGGAGC TGGACTGCTG GGACGGCCCC AACCAGGAAC CCATCATCTA CCATGGCTAC 
ACTTTTACCT CTAAGATACT TTTCTGCGAC GTGCTCAGGG CCATCCGGGA CTACGCCTTC 
AAGGCATCCC CCTACCCAGT TATCCTGTCC CTGGAGAACC ACTGCAGCCT GGAGCAACAA 
CGAGTCATGG CTCATCACCT GAGGGCCATC CTGGGGCCCA TGCTGTTGGA CCAGCCGCTG 
GATGGGGTTA CCACGAGCCT GCCTTCGCCT GAGCAACTGA AGGAAAAGAT TCTGTTGAAA 
GGGAAGAAAC TGGGCGGGCT GCTACCTGCT GGAGGGGAAA ACGGGCCTGA AGCCACCGAT 
GTGTCTGACG AAGATGAGGC TGCTGAAATG GAAGATGAGG CCGTGCGCAG CCAAGTGCAA 
CACAAGCCCA AGGAGGATAA GCTAAAGCTG GTGCCGGAGC TCTCCGACAT GGTCATTTAC 
TGCAAGAGTG TCCACTTTGG TGGCTTCTCC AGTCCTAGCA CCTCTGGGCA GGCATTCTAT 
GAGATGGCTT CCTTCTCCGA GAGCCGTGCC CTGCGGCTGC TGCAAGAATC AGGAAATAGT 
TTTGTCCGCC ATAACGTGGG CCATCTGAGC AGGATCTACC CAGCTGGGTG GAGGACAGAT 
TCCTCCAACT ACAGTCCCGT GGAGATGTGG AATGGAGGCT GCCAGATTGT GGCTCTGAAC 
TTCCAAACCC CTGGGCCAGA AATGGATGTT TACCTTGGCT GCTTCCAGGA CAATGGAGGT 
TGTGGGTATG TGCTGAAACC GGCCTTCCTG AGAGACCCTG ACACCACCTT CAACTCACGG 
GCCCTGACTC AGGGGCCCTG GTGGGCCCCG AAGAAGCTCC GAGTCTGGAT CATCTCTGGA 
CAGCAGCTGC CGAAGGTCAA TAAGAATAAG AATTCTATCG TGGACCCCAA GGTGATAGTG 
GAGATCCACG GTGTGGGCCA GGATGTGGCC AGCCGTCAGA CAGCAGTTAT TACCAATAAT 
GGTTTCAACC CACGGTGGGA CACGGAGTTT GAGTTTGTGG TAGCAGTGCC TGACCTTGCC 
CTTGTACGTT TCATGGTAGA GGATTATGAC TCCTCCTCTA AGAACGACTT TATCGGCCAG 
AGTACTATCC CCTGGAACAG CCTCAAGCAG GGGTACCGCC ATGTCCACCT CTTGTCTAAG 
AATGGAGACC TGCATCCATC AGCCACTCTC TTTGTGAAGA TCTCCATCCA GGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512047.1
CDS	373..2469
Translation	MRSPESQLFSIEDIQEVRMGHRTEGLEKFARDIPEDRCFSIVFKDQRNTLDLIAPSPADVQHWVQGLRKIIDRSGSMDQRQKLQHWIHSCLRKADKNKDNKMNFKEVKDFLKELNVQVDDSYARKIFRECDHSQTDSLEDEEIETFYRMLTQRAEIDRAFAEAAGSAETLSVEKLVTFLQHQQREEEAGPALALSLIERYEPSETAKAQRQMTKDGFLMYLLSADGNAFSLAHRRVYQDMNQPLSHYLVSSSHNTYLLEDQLTGPSSTEAYIRALCKGCRCLELDCWDGPNQEPIIYHGYTFTSKILFCDVLRAIRDYAFKASPYPVILSLENHCSLEQQRVMAHHLRAILGPMLLDQPLDGVTTSLPSPEQLKEKILLKGKKLGGLLPAGGENGPEATDVSDEDEAAEMEDEAVRSQVQHKPKEDKLKLVPELSDMVIYCKSVHFGGFSSPSTSGQAFYEMASFSESRALRLLQESGNSFVRHNVGHLSRIYPAGWRTDSSNYSPVEMWNGGCQIVALNFQTPGPEMDVYLGCFQDNGGCGYVLKPAFLRDPDTTFNSRALTQGPWWAPKKLRVWIISGQQLPKVNKNKNSIVDPKVIVEIHGVGQDVASRQTAVITNNGFNPRWDTEFEFVVAVPDLALVRFMVEDYDSSSKNDFIGQSTIPWNSLKQGYRHVHLLSKNGDLHPSATLFVKISIQD

Target ORF information:

RefSeq Version	XM_006511984.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, delta 1 (Plcd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511984.2

ORF Insert Sequence:

ATGAGGTCCC CGGAGTCGCA GCTGTTCTCC ATCGAGGACA TTCAGGAGGT GCGGATGGGA 
CACCGCACAG AAGGCCTGGA GAAATTTGCC AGAGACATAC CCGAGGACCG ATGCTTCTCC 
ATCGTCTTCA AGGACCAGCG CAATACCCTA GACCTCATCG CCCCGTCTCC AGCTGACGTC 
CAGCATTGGG TGCAGGGCCT GCGCAAGATC ATCGACCGCT CGGGCTCCAT GGACCAGCGG 
CAGAAGCTGC AGCACTGGAT TCACTCCTGC TTGCGAAAGG CTGATAAAAA CAAGGATAAC 
AAGATGAACT TCAAGGAGGT GAAGGACTTC CTGAAGGAGC TCAACGTACA GGTGGATGAC 
AGCTACGCCA GGAAGATCTT CAGGGAATGT GACCACTCCC AGACAGACTC GCTGGAGGAT 
GAGGAGATTG AGACCTTCTA CAGGATGCTG ACCCAGCGGG CAGAGATCGA CCGTGCCTTT 
GCAGAGGCCG CAGGTTCTGC GGAGACCCTG TCGGTGGAGA AGCTAGTGAC GTTCCTGCAG 
CATCAGCAGC GGGAGGAGGA GGCGGGGCCA GCCTTGGCCC TCTCTCTCAT TGAGCGCTAC 
GAGCCCAGTG AGACTGCCAA AGCCCAGCGG CAGATGACCA AGGATGGCTT CCTCATGTAC 
CTGCTGTCTG CTGACGGTAA CGCCTTCAGC CTGGCGCACC GCCGTGTCTA CCAGGACATG 
AACCAGCCGC TCAGCCACTA CTTAGTGTCT TCTTCCCACA ACACCTACCT ACTGGAGGAC 
CAGCTCACAG GGCCCAGCAG CACGGAGGCC TACATCCGGG CTCTGTGCAA AGGCTGCCGG 
TGTCTGGAGC TGGACTGCTG GGACGGCCCC AACCAGGAAC CCATCATCTA CCATGGCTAC 
ACTTTTACCT CTAAGATACT TTTCTGCGAC GTGCTCAGGG CCATCCGGGA CTACGCCTTC 
AAGGCATCCC CCTACCCAGT TATCCTGTCC CTGGAGAACC ACTGCAGCCT GGAGCAACAA 
CGAGTCATGG CTCATCACCT GAGGGCCATC CTGGGGCCCA TGCTGTTGGA CCAGCCGCTG 
GATGGGGTTA CCACGAGCCT GCCTTCGCCT GAGCAACTGA AGGAAAAGAT TCTGTTGAAA 
GGGAAGAAAC TGGGCGGGCT GCTACCTGCT GGAGGGGAAA ACGGGCCTGA AGCCACCGAT 
GTGTCTGACG AAGATGAGGC TGCTGAAATG GAAGATGAGG CCGTGCGCAG CCAAGTGCAA 
CACAAGCCCA AGGAGGATAA GCTAAAGCTG GTGCCGGAGC TCTCCGACAT GGTCATTTAC 
TGCAAGAGTG TCCACTTTGG TGGCTTCTCC AGTCCTAGCA CCTCTGGGCA GGCATTCTAT 
GAGATGGCTT CCTTCTCCGA GAGCCGTGCC CTGCGGCTGC TGCAAGAATC AGGAAATAGT 
TTTGTCCGCC ATAACGTGGG CCATCTGAGC AGGATCTACC CAGCTGGGTG GAGGACAGAT 
TCCTCCAACT ACAGTCCCGT GGAGATGTGG AATGGAGGCT GCCAGATTGT GGCTCTGAAC 
TTCCAAACCC CTGGGCCAGA AATGGATGTT TACCTTGGCT GCTTCCAGGA CAATGGAGGT 
TGTGGGTATG TGCTGAAACC GGCCTTCCTG AGAGACCCTG ACACCACCTT CAACTCACGG 
GCCCTGACTC AGGGGCCCTG GTGGGCCCCG AAGAAGCTCC GAGTCTGGAT CATCTCTGGA 
CAGCAGCTGC CGAAGGTCAA TAAGAATAAG AATTCTATCG TGGACCCCAA GGTGATAGTG 
GAGATCCACG GTGTGGGCCA GGATGTGGCC AGCCGTCAGA CAGCAGTTAT TACCAATAAT 
GGTTTCAACC CACGGTGGGA CACGGAGTTT GAGTTTGTGG TAGCAGTGCC TGACCTTGCC 
CTTGTACGTT TCATGGTAGA GGATTATGAC TCCTCCTCTA AGAACGACTT TATCGGCCAG 
AGTACTATCC CCTGGAACAG CCTCAAGCAG GGGTACCGCC ATGTCCACCT CTTGTCTAAG 
AATGGAGACC TGCATCCATC AGCCACTCTC TTTGTGAAGA TCTCCATCCA GGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plcd1 ( XM_006511984.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512047.1 Mus musculus phospholipase C, delta 1 (Plcd1), transcript variant X1, mRNA.

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CloneID	OMu43330
Clone ID Related Accession (Same CDS sequence)	XM_006511984.3 , XM_006511984.2
Accession Version	XM_006511984.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006511984.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTCCC CGGAGTCGCA GCTGTTCTCC ATCGAGGACA TTCAGGAGGT GCGGATGGGA 
CACCGCACAG AAGGCCTGGA GAAATTTGCC AGAGACATAC CCGAGGACCG ATGCTTCTCC 
ATCGTCTTCA AGGACCAGCG CAATACCCTA GACCTCATCG CCCCGTCTCC AGCTGACGTC 
CAGCATTGGG TGCAGGGCCT GCGCAAGATC ATCGACCGCT CGGGCTCCAT GGACCAGCGG 
CAGAAGCTGC AGCACTGGAT TCACTCCTGC TTGCGAAAGG CTGATAAAAA CAAGGATAAC 
AAGATGAACT TCAAGGAGGT GAAGGACTTC CTGAAGGAGC TCAACGTACA GGTGGATGAC 
AGCTACGCCA GGAAGATCTT CAGGGAATGT GACCACTCCC AGACAGACTC GCTGGAGGAT 
GAGGAGATTG AGACCTTCTA CAGGATGCTG ACCCAGCGGG CAGAGATCGA CCGTGCCTTT 
GCAGAGGCCG CAGGTTCTGC GGAGACCCTG TCGGTGGAGA AGCTAGTGAC GTTCCTGCAG 
CATCAGCAGC GGGAGGAGGA GGCGGGGCCA GCCTTGGCCC TCTCTCTCAT TGAGCGCTAC 
GAGCCCAGTG AGACTGCCAA AGCCCAGCGG CAGATGACCA AGGATGGCTT CCTCATGTAC 
CTGCTGTCTG CTGACGGTAA CGCCTTCAGC CTGGCGCACC GCCGTGTCTA CCAGGACATG 
AACCAGCCGC TCAGCCACTA CTTAGTGTCT TCTTCCCACA ACACCTACCT ACTGGAGGAC 
CAGCTCACAG GGCCCAGCAG CACGGAGGCC TACATCCGGG CTCTGTGCAA AGGCTGCCGG 
TGTCTGGAGC TGGACTGCTG GGACGGCCCC AACCAGGAAC CCATCATCTA CCATGGCTAC 
ACTTTTACCT CTAAGATACT TTTCTGCGAC GTGCTCAGGG CCATCCGGGA CTACGCCTTC 
AAGGCATCCC CCTACCCAGT TATCCTGTCC CTGGAGAACC ACTGCAGCCT GGAGCAACAA 
CGAGTCATGG CTCATCACCT GAGGGCCATC CTGGGGCCCA TGCTGTTGGA CCAGCCGCTG 
GATGGGGTTA CCACGAGCCT GCCTTCGCCT GAGCAACTGA AGGAAAAGAT TCTGTTGAAA 
GGGAAGAAAC TGGGCGGGCT GCTACCTGCT GGAGGGGAAA ACGGGCCTGA AGCCACCGAT 
GTGTCTGACG AAGATGAGGC TGCTGAAATG GAAGATGAGG CCGTGCGCAG CCAAGTGCAA 
CACAAGCCCA AGGAGGATAA GCTAAAGCTG GTGCCGGAGC TCTCCGACAT GGTCATTTAC 
TGCAAGAGTG TCCACTTTGG TGGCTTCTCC AGTCCTAGCA CCTCTGGGCA GGCATTCTAT 
GAGATGGCTT CCTTCTCCGA GAGCCGTGCC CTGCGGCTGC TGCAAGAATC AGGAAATAGT 
TTTGTCCGCC ATAACGTGGG CCATCTGAGC AGGATCTACC CAGCTGGGTG GAGGACAGAT 
TCCTCCAACT ACAGTCCCGT GGAGATGTGG AATGGAGGCT GCCAGATTGT GGCTCTGAAC 
TTCCAAACCC CTGGGCCAGA AATGGATGTT TACCTTGGCT GCTTCCAGGA CAATGGAGGT 
TGTGGGTATG TGCTGAAACC GGCCTTCCTG AGAGACCCTG ACACCACCTT CAACTCACGG 
GCCCTGACTC AGGGGCCCTG GTGGGCCCCG AAGAAGCTCC GAGTCTGGAT CATCTCTGGA 
CAGCAGCTGC CGAAGGTCAA TAAGAATAAG AATTCTATCG TGGACCCCAA GGTGATAGTG 
GAGATCCACG GTGTGGGCCA GGATGTGGCC AGCCGTCAGA CAGCAGTTAT TACCAATAAT 
GGTTTCAACC CACGGTGGGA CACGGAGTTT GAGTTTGTGG TAGCAGTGCC TGACCTTGCC 
CTTGTACGTT TCATGGTAGA GGATTATGAC TCCTCCTCTA AGAACGACTT TATCGGCCAG 
AGTACTATCC CCTGGAACAG CCTCAAGCAG GGGTACCGCC ATGTCCACCT CTTGTCTAAG 
AATGGAGACC TGCATCCATC AGCCACTCTC TTTGTGAAGA TCTCCATCCA GGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512047.1
CDS	366..2462
Translation	MRSPESQLFSIEDIQEVRMGHRTEGLEKFARDIPEDRCFSIVFKDQRNTLDLIAPSPADVQHWVQGLRKIIDRSGSMDQRQKLQHWIHSCLRKADKNKDNKMNFKEVKDFLKELNVQVDDSYARKIFRECDHSQTDSLEDEEIETFYRMLTQRAEIDRAFAEAAGSAETLSVEKLVTFLQHQQREEEAGPALALSLIERYEPSETAKAQRQMTKDGFLMYLLSADGNAFSLAHRRVYQDMNQPLSHYLVSSSHNTYLLEDQLTGPSSTEAYIRALCKGCRCLELDCWDGPNQEPIIYHGYTFTSKILFCDVLRAIRDYAFKASPYPVILSLENHCSLEQQRVMAHHLRAILGPMLLDQPLDGVTTSLPSPEQLKEKILLKGKKLGGLLPAGGENGPEATDVSDEDEAAEMEDEAVRSQVQHKPKEDKLKLVPELSDMVIYCKSVHFGGFSSPSTSGQAFYEMASFSESRALRLLQESGNSFVRHNVGHLSRIYPAGWRTDSSNYSPVEMWNGGCQIVALNFQTPGPEMDVYLGCFQDNGGCGYVLKPAFLRDPDTTFNSRALTQGPWWAPKKLRVWIISGQQLPKVNKNKNSIVDPKVIVEIHGVGQDVASRQTAVITNNGFNPRWDTEFEFVVAVPDLALVRFMVEDYDSSSKNDFIGQSTIPWNSLKQGYRHVHLLSKNGDLHPSATLFVKISIQD

Target ORF information:

RefSeq Version	XM_006511984.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, delta 1 (Plcd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511984.3

ORF Insert Sequence:

ATGAGGTCCC CGGAGTCGCA GCTGTTCTCC ATCGAGGACA TTCAGGAGGT GCGGATGGGA 
CACCGCACAG AAGGCCTGGA GAAATTTGCC AGAGACATAC CCGAGGACCG ATGCTTCTCC 
ATCGTCTTCA AGGACCAGCG CAATACCCTA GACCTCATCG CCCCGTCTCC AGCTGACGTC 
CAGCATTGGG TGCAGGGCCT GCGCAAGATC ATCGACCGCT CGGGCTCCAT GGACCAGCGG 
CAGAAGCTGC AGCACTGGAT TCACTCCTGC TTGCGAAAGG CTGATAAAAA CAAGGATAAC 
AAGATGAACT TCAAGGAGGT GAAGGACTTC CTGAAGGAGC TCAACGTACA GGTGGATGAC 
AGCTACGCCA GGAAGATCTT CAGGGAATGT GACCACTCCC AGACAGACTC GCTGGAGGAT 
GAGGAGATTG AGACCTTCTA CAGGATGCTG ACCCAGCGGG CAGAGATCGA CCGTGCCTTT 
GCAGAGGCCG CAGGTTCTGC GGAGACCCTG TCGGTGGAGA AGCTAGTGAC GTTCCTGCAG 
CATCAGCAGC GGGAGGAGGA GGCGGGGCCA GCCTTGGCCC TCTCTCTCAT TGAGCGCTAC 
GAGCCCAGTG AGACTGCCAA AGCCCAGCGG CAGATGACCA AGGATGGCTT CCTCATGTAC 
CTGCTGTCTG CTGACGGTAA CGCCTTCAGC CTGGCGCACC GCCGTGTCTA CCAGGACATG 
AACCAGCCGC TCAGCCACTA CTTAGTGTCT TCTTCCCACA ACACCTACCT ACTGGAGGAC 
CAGCTCACAG GGCCCAGCAG CACGGAGGCC TACATCCGGG CTCTGTGCAA AGGCTGCCGG 
TGTCTGGAGC TGGACTGCTG GGACGGCCCC AACCAGGAAC CCATCATCTA CCATGGCTAC 
ACTTTTACCT CTAAGATACT TTTCTGCGAC GTGCTCAGGG CCATCCGGGA CTACGCCTTC 
AAGGCATCCC CCTACCCAGT TATCCTGTCC CTGGAGAACC ACTGCAGCCT GGAGCAACAA 
CGAGTCATGG CTCATCACCT GAGGGCCATC CTGGGGCCCA TGCTGTTGGA CCAGCCGCTG 
GATGGGGTTA CCACGAGCCT GCCTTCGCCT GAGCAACTGA AGGAAAAGAT TCTGTTGAAA 
GGGAAGAAAC TGGGCGGGCT GCTACCTGCT GGAGGGGAAA ACGGGCCTGA AGCCACCGAT 
GTGTCTGACG AAGATGAGGC TGCTGAAATG GAAGATGAGG CCGTGCGCAG CCAAGTGCAA 
CACAAGCCCA AGGAGGATAA GCTAAAGCTG GTGCCGGAGC TCTCCGACAT GGTCATTTAC 
TGCAAGAGTG TCCACTTTGG TGGCTTCTCC AGTCCTAGCA CCTCTGGGCA GGCATTCTAT 
GAGATGGCTT CCTTCTCCGA GAGCCGTGCC CTGCGGCTGC TGCAAGAATC AGGAAATAGT 
TTTGTCCGCC ATAACGTGGG CCATCTGAGC AGGATCTACC CAGCTGGGTG GAGGACAGAT 
TCCTCCAACT ACAGTCCCGT GGAGATGTGG AATGGAGGCT GCCAGATTGT GGCTCTGAAC 
TTCCAAACCC CTGGGCCAGA AATGGATGTT TACCTTGGCT GCTTCCAGGA CAATGGAGGT 
TGTGGGTATG TGCTGAAACC GGCCTTCCTG AGAGACCCTG ACACCACCTT CAACTCACGG 
GCCCTGACTC AGGGGCCCTG GTGGGCCCCG AAGAAGCTCC GAGTCTGGAT CATCTCTGGA 
CAGCAGCTGC CGAAGGTCAA TAAGAATAAG AATTCTATCG TGGACCCCAA GGTGATAGTG 
GAGATCCACG GTGTGGGCCA GGATGTGGCC AGCCGTCAGA CAGCAGTTAT TACCAATAAT 
GGTTTCAACC CACGGTGGGA CACGGAGTTT GAGTTTGTGG TAGCAGTGCC TGACCTTGCC 
CTTGTACGTT TCATGGTAGA GGATTATGAC TCCTCCTCTA AGAACGACTT TATCGGCCAG 
AGTACTATCC CCTGGAACAG CCTCAAGCAG GGGTACCGCC ATGTCCACCT CTTGTCTAAG 
AATGGAGACC TGCATCCATC AGCCACTCTC TTTGTGAAGA TCTCCATCCA GGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Actomyosin polarisation through PLC-PKC triggers symmetry breaking of the mouse embryo.
Nature communications8(1)921(2017 Oct)
Zhu M,Leung CY,Shahbazi MN,Zernicka-Goetz M

Phospholipase C?1 regulates p38 MAPK activity and skin barrier integrity.
Cell death and differentiation24(6)1079-1090(2017 Jun)
Kanemaru K,Nakamura Y,Totoki K,Fukuyama T,Shoji M,Kaneko H,Shiratori K,Yoneda A,Inoue T,Iwakura Y,Kabashima K,Fukami K

Karyopherin Alpha 1 Regulates Satellite Cell Proliferation and Survival by Modulating Nuclear Import.
Stem cells (Dayton, Ohio)34(11)2784-2797(2016 Nov)
Choo HJ,Cutler A,Rother F,Bader M,Pavlath GK

[Phospholipase C ?1 (PLCD1) inhibits the proliferation, invasion and migration of CAPAN-1 and BXPC-3 pancreatic cancer cells].
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology32(6)739-45(2016 Jun)
Hu D,Jiang Z

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Plcd1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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