PLCD1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLCD1
Entrez Gene ID	5333
Full Name	phospholipase C delta 1
Synonyms	NDNC3,PLC-III
General protein information	Preferred Names phospholipase C delta 1 Names 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 PLC-delta-1 phosphoinositide phospholipase C-delta-1 phospholipase C-III
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p22.2
Summary	This gene encodes a member of the phospholipase C family. Phospholipase C isozymes play critical roles in intracellular signal transduction by catalyzing the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) into the second messengers diacylglycerol (DAG) and inositol triphosphate (IP3). The encoded protein functions as a tumor suppressor in several types of cancer, and mutations in this gene are a cause of hereditary leukonychia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011].
Disorder MIM:	602142
Disorder Html:	Nail disorder, nonsyndromic congenital, 3, (leukonychia), 151600 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001130964.2	NP_001124436.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 isoform 1
XM_017006622.1	XP_016862111.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 isoform X1
NM_001130964.1	NP_001124436.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 isoform 1
NM_006225.3	NP_006216.2	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 isoform 2
NM_006225.4	NP_006216.2	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 isoform 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Plcd1	NP_062650.1
Homo sapiens (human)	PLCD1	NP_001124436.1
	PLCD1	XP_001089399.1
Oryza sativa (rice)	Os05g0127200	NP_001054531.1
Pan troglodytes (chimpanzee)	PLCD1	XP_003309769.2
Rattus norvegicus (Norway rat)	Plcd1	NP_058731.1
Canis lupus familiaris (dog)	PLCD1	XP_859460.2
Xenopus tropicalis (tropical clawed frog)	plcd1	XP_004915652.1
Bos taurus (cattle)	PLCD1	NP_001075045.1
Gallus gallus (chicken)	PLCD1	XP_418522.2

Gene Ontology
Bibliography

Related articles in PubMed

Mutations of candidate tumor suppressor genes at chromosome 3p in intrahepatic cholangiocarcinoma.
You HL, Huang WT, Liu TT, Weng SW, Eng HL
Experimental and molecular pathology103(3)249-254(2017 12)

Phospholipase C ?1 negatively regulates autophagy in colorectal cancer cells.
Shimozawa M, Anzai S, Satow R, Fukami K
Biochemical and biophysical research communications488(4)578-583(2017 07)

Phospholipase C?1 regulates p38 MAPK activity and skin barrier integrity.
Kanemaru K, Nakamura Y, Totoki K, Fukuyama T, Shoji M, Kaneko H, Shiratori K, Yoneda A, Inoue T, Iwakura Y, Kabashima K, Fukami K
Cell death and differentiation24(6)1079-1090(2017 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nine of the 32 (28.1%) iCCA patients had gene mutations at chromosome 3p, totaling 11 mutations across five genes. Those included five (15.6%) BAP1 mutations, two each (6.3%) of CACNA2D3 and RASSF1 mutations, and one each (3.1%) of ATG7 and PLCD1 mutations. Six (18.8%) cases had concurrent loss of chromosome 3p and gene mutations.
Title: Mutations of candidate tumor suppressor genes at chromosome 3p in intrahepatic cholangiocarcinoma.

Phospholipase C delta 1 is both a KLF15-regulated gene and a novel repressor of airway smooth muscle hypertrophy.
Title: Glucocorticoid Receptor ChIP-Seq Identifies PLCD1 as a KLF15 Target that Represses Airway Smooth Muscle Hypertrophy.

The PLCdelta1 negatively regulates autophagy, and PLCdelta1 suppression contributes to the tolerance of CRC cells harboring KRAS mutations to nutrient deprivation and anti-cancer drug treatment.
Title: Phospholipase C ?1 negatively regulates autophagy in colorectal cancer cells.

High PLC delta expression is associated with breast cancer.
Title: Expression of phospholipase C isozymes in human breast cancer and their clinical significance.

slow phase of Gi/o-mediated TRPC4 activation was diminished by inhibiting RhoA or enhancing PLCdelta function
Title: Critical roles of Gi/o proteins and phospholipase C-?1 in the activation of receptor-operated TRPC4 channels.

The following PLCD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLCD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26150	NM_006225.4 Latest version!	Homo sapiens phospholipase C delta 1 (PLCD1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu26435	NM_001130964.2 Latest version!	Homo sapiens phospholipase C delta 1 (PLCD1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$389.50-$545.30 $779.00
OHu94289	XM_017006622.1 Latest version!	Homo sapiens phospholipase C delta 1 (PLCD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$391.30 $559.00
View Old Accession Versions >>
OHu26435	NM_001130964.1	Homo sapiens phospholipase C delta 1 (PLCD1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$389.50-$545.30 $779.00
OHu26150	NM_006225.3	Homo sapiens phospholipase C delta 1 (PLCD1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLCD1 ( NM_006225.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006216.2 Homo sapiens phospholipase C delta 1 (PLCD1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu26150
Clone ID Related Accession (Same CDS sequence)	NM_006225.3 , NM_006225.4
Accession Version	NM_006225.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK292324.1 and BC050382.1. This sequence is a reference standard in the RefSeqGene project. On Aug 9, 2008 this sequence version replaced NM_006225.2. Transcript Variant: This variant (2) contains an alternate 5' terminal exon compared to transcript variant 1, and initiates translation from an in-frame upstream AUG, resulting in a shorter isoform (2) with a different N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292324.1, SRR1803611.71732.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACTCGG GCCGGGACTT CCTGACCCTG CACGGCCTAC AGGATGATGA GGATCTACAG 
GCGCTGCTGA AGGGCAGCCA GCTCCTGAAG GTGAAGTCCA GCTCATGGAG GAGAGAGCGC 
TTCTACAAGT TGCAGGAGGA CTGCAAGACC ATCTGGCAGG AGTCCCGCAA GGTCATGCGG 
ACCCCGGAGT CCCAGCTGTT CTCCATCGAG GACATTCAGG AGGTGCGAAT GGGGCACCGC 
ACGGAGGGTC TGGAGAAGTT CGCCCGTGAT GTGCCCGAGG ACCGCTGCTT CTCCATTGTC 
TTCAAGGACC AGCGCAATAC ACTAGACCTC ATCGCCCCAT CGCCAGCTGA TGCCCAGCAC 
TGGGTGCTGG GGCTGCACAA GATCATCCAC CACTCAGGCT CCATGGACCA GCGTCAGAAG 
CTACAGCACT GGATTCACTC CTGCTTGCGA AAAGCTGACA AAAACAAGGA CAACAAGATG 
AGCTTCAAGG AGCTGCAGAA CTTCCTGAAG GAGCTCAACA TCCAGGTGGA CGACAGCTAT 
GCCCGGAAGA TCTTCAGGGA GTGTGACCAC TCCCAGACAG ACTCCCTGGA GGACGAGGAG 
ATTGAGGCCT TCTACAAGAT GCTGACCCAG CGGGTGGAGA TCGACCGCAC CTTCGCCGAG 
GCCGCGGGCT CAGGGGAGAC TCTGTCGGTG GATCAGTTAG TGACGTTCCT GCAGCACCAG 
CAGCGGGAGG AGGCGGCAGG GCCTGCGCTG GCCCTCTCCC TCATTGAGCG CTACGAGCCC 
AGCGAGACTG CCAAGGCGCA GCGGCAGATG ACCAAGGACG GCTTCCTCAT GTACTTACTG 
TCGGCTGACG GCAGCGCCTT CAGCCTGGCA CACCGCCGTG TCTACCAGGA CATGGGCCAG 
CCACTTAGCC ACTACCTGGT GTCCTCTTCA CACAACACCT ACCTGCTGGA GGACCAGCTA 
GCCGGGCCCA GCAGCACTGA AGCCTACATC CGGGCACTGT GCAAAGGCTG CCGATGCCTG 
GAGCTTGACT GCTGGGACGG GCCCAACCAG GAACCAATCA TCTACCACGG CTATACTTTC 
ACTTCCAAGA TCCTCTTCTG CGATGTGCTC AGGGCCATCC GGGACTATGC CTTCAAGGCG 
TCCCCCTACC CTGTCATCCT ATCCCTGGAG AACCACTGCA CACTGGAGCA GCAGCGCGTG 
ATGGCGCGGC ACCTGCATGC CATCCTGGGC CCCATGCTGT TGAACCGACC ACTGGATGGG 
GTCACCAACA GCCTGCCCTC CCCTGAGCAA CTGAAGGGGA AGATCCTGCT GAAGGGGAAG 
AAGCTCGGGG GGCTCCTGCC CCCTGGAGGG GAGGGTGGCC CTGAGGCCAC TGTGGTGTCA 
GACGAAGACG AGGCTGCTGA GATGGAGGAT GAGGCAGTGA GGAGCCGTGT GCAGCACAAG 
CCCAAGGAGG ACAAGCTCAG GCTAGCACAG GAGCTCTCTG ACATGGTCAT TTACTGCAAG 
AGTGTCCACT TTGGGGGCTT CTCCAGTCCT GGCACCCCTG GACAGGCCTT CTACGAGATG 
GCGTCCTTCT CTGAGAACCG TGCCCTTCGA CTGCTCCAAG AATCAGGAAA CGGCTTTGTC 
CGCCACAACG TGGGGCACCT GAGCAGAATC TACCCGGCTG GATGGAGAAC AGACTCCTCC 
AACTACAGCC CCGTGGAGAT GTGGAATGGG GGCTGCCAGA TCGTGGCCCT GAATTTCCAG 
ACACCTGGGC CAGAGATGGA CGTGTACCAG GGCCGCTTCC AGGACAACGG GGCCTGTGGG 
TACGTGCTGA AGCCCGCCTT CCTGCGAGAC CCCAACGGCA CCTTTAACCC CCGCGCCCTG 
GCTCAGGGGC CCTGGTGGGC ACGGAAGCGG CTCAACATCA GGGTCATTTC GGGGCAGCAG 
CTGCCAAAAG TCAACAAGAA TAAGAATTCA ATTGTGGACC CCAAAGTGAC AGTGGAGATC 
CATGGCGTGA GCCGGGACGT GGCCAGCCGC CAGACTGCTG TCATCACCAA CAATGGTTTC 
AACCCATGGT GGGACACGGA GTTTGCGTTT GAGGTAGTTG TGCCTGACCT TGCCCTCATC 
CGCTTCTTGG TGGAAGATTA TGATGCCTCC TCCAAGAATG ACTTCATTGG CCAGAGTACC 
ATCCCCTTGA ACAGCCTCAA GCAAGGATAC CGCCATGTCC ACCTCATGTC TAAGAACGGG 
GACCAGCATC CATCAGCCAC CCTCTTTGTG AAGATCTCCC TCCAGGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006216.2
CDS	125..2395
Translation	MDSGRDFLTLHGLQDDEDLQALLKGSQLLKVKSSSWRRERFYKLQEDCKTIWQESRKVMRTPESQLFSIEDIQEVRMGHRTEGLEKFARDVPEDRCFSIVFKDQRNTLDLIAPSPADAQHWVLGLHKIIHHSGSMDQRQKLQHWIHSCLRKADKNKDNKMSFKELQNFLKELNIQVDDSYARKIFRECDHSQTDSLEDEEIEAFYKMLTQRVEIDRTFAEAAGSGETLSVDQLVTFLQHQQREEAAGPALALSLIERYEPSETAKAQRQMTKDGFLMYLLSADGSAFSLAHRRVYQDMGQPLSHYLVSSSHNTYLLEDQLAGPSSTEAYIRALCKGCRCLELDCWDGPNQEPIIYHGYTFTSKILFCDVLRAIRDYAFKASPYPVILSLENHCTLEQQRVMARHLHAILGPMLLNRPLDGVTNSLPSPEQLKGKILLKGKKLGGLLPPGGEGGPEATVVSDEDEAAEMEDEAVRSRVQHKPKEDKLRLAQELSDMVIYCKSVHFGGFSSPGTPGQAFYEMASFSENRALRLLQESGNGFVRHNVGHLSRIYPAGWRTDSSNYSPVEMWNGGCQIVALNFQTPGPEMDVYQGRFQDNGACGYVLKPAFLRDPNGTFNPRALAQGPWWARKRLNIRVISGQQLPKVNKNKNSIVDPKVTVEIHGVSRDVASRQTAVITNNGFNPWWDTEFAFEVVVPDLALIRFLVEDYDASSKNDFIGQSTIPLNSLKQGYRHVHLMSKNGDQHPSATLFVKISLQD

Target ORF information:

RefSeq Version	NM_006225.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C delta 1 (PLCD1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006225.3

ORF Insert Sequence:

ATGGACTCGG GCCGGGACTT CCTGACCCTG CACGGCCTAC AGGATGATGA GGATCTACAG 
GCGCTGCTGA AGGGCAGCCA GCTCCTGAAG GTGAAGTCCA GCTCATGGAG GAGAGAGCGC 
TTCTACAAGT TGCAGGAGGA CTGCAAGACC ATCTGGCAGG AGTCCCGCAA GGTCATGCGG 
ACCCCGGAGT CCCAGCTGTT CTCCATCGAG GACATTCAGG AGGTGCGAAT GGGGCACCGC 
ACGGAGGGTC TGGAGAAGTT CGCCCGTGAT GTGCCCGAGG ACCGCTGCTT CTCCATTGTC 
TTCAAGGACC AGCGCAATAC ACTAGACCTC ATCGCCCCAT CGCCAGCTGA TGCCCAGCAC 
TGGGTGCTGG GGCTGCACAA GATCATCCAC CACTCAGGCT CCATGGACCA GCGTCAGAAG 
CTACAGCACT GGATTCACTC CTGCTTGCGA AAAGCTGACA AAAACAAGGA CAACAAGATG 
AGCTTCAAGG AGCTGCAGAA CTTCCTGAAG GAGCTCAACA TCCAGGTGGA CGACAGCTAT 
GCCCGGAAGA TCTTCAGGGA GTGTGACCAC TCCCAGACAG ACTCCCTGGA GGACGAGGAG 
ATTGAGGCCT TCTACAAGAT GCTGACCCAG CGGGTGGAGA TCGACCGCAC CTTCGCCGAG 
GCCGCGGGCT CAGGGGAGAC TCTGTCGGTG GATCAGTTAG TGACGTTCCT GCAGCACCAG 
CAGCGGGAGG AGGCGGCAGG GCCTGCGCTG GCCCTCTCCC TCATTGAGCG CTACGAGCCC 
AGCGAGACTG CCAAGGCGCA GCGGCAGATG ACCAAGGACG GCTTCCTCAT GTACTTACTG 
TCGGCTGACG GCAGCGCCTT CAGCCTGGCA CACCGCCGTG TCTACCAGGA CATGGGCCAG 
CCACTTAGCC ACTACCTGGT GTCCTCTTCA CACAACACCT ACCTGCTGGA GGACCAGCTA 
GCCGGGCCCA GCAGCACTGA AGCCTACATC CGGGCACTGT GCAAAGGCTG CCGATGCCTG 
GAGCTTGACT GCTGGGACGG GCCCAACCAG GAACCAATCA TCTACCACGG CTATACTTTC 
ACTTCCAAGA TCCTCTTCTG CGATGTGCTC AGGGCCATCC GGGACTATGC CTTCAAGGCG 
TCCCCCTACC CTGTCATCCT ATCCCTGGAG AACCACTGCA CACTGGAGCA GCAGCGCGTG 
ATGGCGCGGC ACCTGCATGC CATCCTGGGC CCCATGCTGT TGAACCGACC ACTGGATGGG 
GTCACCAACA GCCTGCCCTC CCCTGAGCAA CTGAAGGGGA AGATCCTGCT GAAGGGGAAG 
AAGCTCGGGG GGCTCCTGCC CCCTGGAGGG GAGGGTGGCC CTGAGGCCAC TGTGGTGTCA 
GACGAAGACG AGGCTGCTGA GATGGAGGAT GAGGCAGTGA GGAGCCGTGT GCAGCACAAG 
CCCAAGGAGG ACAAGCTCAG GCTAGCACAG GAGCTCTCTG ACATGGTCAT TTACTGCAAG 
AGTGTCCACT TTGGGGGCTT CTCCAGTCCT GGCACCCCTG GACAGGCCTT CTACGAGATG 
GCGTCCTTCT CTGAGAACCG TGCCCTTCGA CTGCTCCAAG AATCAGGAAA CGGCTTTGTC 
CGCCACAACG TGGGGCACCT GAGCAGAATC TACCCGGCTG GATGGAGAAC AGACTCCTCC 
AACTACAGCC CCGTGGAGAT GTGGAATGGG GGCTGCCAGA TCGTGGCCCT GAATTTCCAG 
ACACCTGGGC CAGAGATGGA CGTGTACCAG GGCCGCTTCC AGGACAACGG GGCCTGTGGG 
TACGTGCTGA AGCCCGCCTT CCTGCGAGAC CCCAACGGCA CCTTTAACCC CCGCGCCCTG 
GCTCAGGGGC CCTGGTGGGC ACGGAAGCGG CTCAACATCA GGGTCATTTC GGGGCAGCAG 
CTGCCAAAAG TCAACAAGAA TAAGAATTCA ATTGTGGACC CCAAAGTGAC AGTGGAGATC 
CATGGCGTGA GCCGGGACGT GGCCAGCCGC CAGACTGCTG TCATCACCAA CAATGGTTTC 
AACCCATGGT GGGACACGGA GTTTGCGTTT GAGGTAGTTG TGCCTGACCT TGCCCTCATC 
CGCTTCTTGG TGGAAGATTA TGATGCCTCC TCCAAGAATG ACTTCATTGG CCAGAGTACC 
ATCCCCTTGA ACAGCCTCAA GCAAGGATAC CGCCATGTCC ACCTCATGTC TAAGAACGGG 
GACCAGCATC CATCAGCCAC CCTCTTTGTG AAGATCTCCC TCCAGGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCD1 ( NM_006225.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006216.2 Homo sapiens phospholipase C delta 1 (PLCD1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu26150
Clone ID Related Accession (Same CDS sequence)	NM_006225.3 , NM_006225.4
Accession Version	NM_006225.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK292324.1 and BC050382.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_006225.3. Transcript Variant: This variant (2) contains an alternate 5' terminal exon compared to transcript variant 1, and initiates translation from an in-frame upstream AUG, resulting in a shorter isoform (2) with a different N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GQ900974.1, BC050382.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGACTCGG GCCGGGACTT CCTGACCCTG CACGGCCTAC AGGATGATGA GGATCTACAG 
GCGCTGCTGA AGGGCAGCCA GCTCCTGAAG GTGAAGTCCA GCTCATGGAG GAGAGAGCGC 
TTCTACAAGT TGCAGGAGGA CTGCAAGACC ATCTGGCAGG AGTCCCGCAA GGTCATGCGG 
ACCCCGGAGT CCCAGCTGTT CTCCATCGAG GACATTCAGG AGGTGCGAAT GGGGCACCGC 
ACGGAGGGTC TGGAGAAGTT CGCCCGTGAT GTGCCCGAGG ACCGCTGCTT CTCCATTGTC 
TTCAAGGACC AGCGCAATAC ACTAGACCTC ATCGCCCCAT CGCCAGCTGA TGCCCAGCAC 
TGGGTGCTGG GGCTGCACAA GATCATCCAC CACTCAGGCT CCATGGACCA GCGTCAGAAG 
CTACAGCACT GGATTCACTC CTGCTTGCGA AAAGCTGACA AAAACAAGGA CAACAAGATG 
AGCTTCAAGG AGCTGCAGAA CTTCCTGAAG GAGCTCAACA TCCAGGTGGA CGACAGCTAT 
GCCCGGAAGA TCTTCAGGGA GTGTGACCAC TCCCAGACAG ACTCCCTGGA GGACGAGGAG 
ATTGAGGCCT TCTACAAGAT GCTGACCCAG CGGGTGGAGA TCGACCGCAC CTTCGCCGAG 
GCCGCGGGCT CAGGGGAGAC TCTGTCGGTG GATCAGTTAG TGACGTTCCT GCAGCACCAG 
CAGCGGGAGG AGGCGGCAGG GCCTGCGCTG GCCCTCTCCC TCATTGAGCG CTACGAGCCC 
AGCGAGACTG CCAAGGCGCA GCGGCAGATG ACCAAGGACG GCTTCCTCAT GTACTTACTG 
TCGGCTGACG GCAGCGCCTT CAGCCTGGCA CACCGCCGTG TCTACCAGGA CATGGGCCAG 
CCACTTAGCC ACTACCTGGT GTCCTCTTCA CACAACACCT ACCTGCTGGA GGACCAGCTA 
GCCGGGCCCA GCAGCACTGA AGCCTACATC CGGGCACTGT GCAAAGGCTG CCGATGCCTG 
GAGCTTGACT GCTGGGACGG GCCCAACCAG GAACCAATCA TCTACCACGG CTATACTTTC 
ACTTCCAAGA TCCTCTTCTG CGATGTGCTC AGGGCCATCC GGGACTATGC CTTCAAGGCG 
TCCCCCTACC CTGTCATCCT ATCCCTGGAG AACCACTGCA CACTGGAGCA GCAGCGCGTG 
ATGGCGCGGC ACCTGCATGC CATCCTGGGC CCCATGCTGT TGAACCGACC ACTGGATGGG 
GTCACCAACA GCCTGCCCTC CCCTGAGCAA CTGAAGGGGA AGATCCTGCT GAAGGGGAAG 
AAGCTCGGGG GGCTCCTGCC CCCTGGAGGG GAGGGTGGCC CTGAGGCCAC TGTGGTGTCA 
GACGAAGACG AGGCTGCTGA GATGGAGGAT GAGGCAGTGA GGAGCCGTGT GCAGCACAAG 
CCCAAGGAGG ACAAGCTCAG GCTAGCACAG GAGCTCTCTG ACATGGTCAT TTACTGCAAG 
AGTGTCCACT TTGGGGGCTT CTCCAGTCCT GGCACCCCTG GACAGGCCTT CTACGAGATG 
GCGTCCTTCT CTGAGAACCG TGCCCTTCGA CTGCTCCAAG AATCAGGAAA CGGCTTTGTC 
CGCCACAACG TGGGGCACCT GAGCAGAATC TACCCGGCTG GATGGAGAAC AGACTCCTCC 
AACTACAGCC CCGTGGAGAT GTGGAATGGG GGCTGCCAGA TCGTGGCCCT GAATTTCCAG 
ACACCTGGGC CAGAGATGGA CGTGTACCAG GGCCGCTTCC AGGACAACGG GGCCTGTGGG 
TACGTGCTGA AGCCCGCCTT CCTGCGAGAC CCCAACGGCA CCTTTAACCC CCGCGCCCTG 
GCTCAGGGGC CCTGGTGGGC ACGGAAGCGG CTCAACATCA GGGTCATTTC GGGGCAGCAG 
CTGCCAAAAG TCAACAAGAA TAAGAATTCA ATTGTGGACC CCAAAGTGAC AGTGGAGATC 
CATGGCGTGA GCCGGGACGT GGCCAGCCGC CAGACTGCTG TCATCACCAA CAATGGTTTC 
AACCCATGGT GGGACACGGA GTTTGCGTTT GAGGTAGTTG TGCCTGACCT TGCCCTCATC 
CGCTTCTTGG TGGAAGATTA TGATGCCTCC TCCAAGAATG ACTTCATTGG CCAGAGTACC 
ATCCCCTTGA ACAGCCTCAA GCAAGGATAC CGCCATGTCC ACCTCATGTC TAAGAACGGG 
GACCAGCATC CATCAGCCAC CCTCTTTGTG AAGATCTCCC TCCAGGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006216.2
CDS	104..2374
Translation	MDSGRDFLTLHGLQDDEDLQALLKGSQLLKVKSSSWRRERFYKLQEDCKTIWQESRKVMRTPESQLFSIEDIQEVRMGHRTEGLEKFARDVPEDRCFSIVFKDQRNTLDLIAPSPADAQHWVLGLHKIIHHSGSMDQRQKLQHWIHSCLRKADKNKDNKMSFKELQNFLKELNIQVDDSYARKIFRECDHSQTDSLEDEEIEAFYKMLTQRVEIDRTFAEAAGSGETLSVDQLVTFLQHQQREEAAGPALALSLIERYEPSETAKAQRQMTKDGFLMYLLSADGSAFSLAHRRVYQDMGQPLSHYLVSSSHNTYLLEDQLAGPSSTEAYIRALCKGCRCLELDCWDGPNQEPIIYHGYTFTSKILFCDVLRAIRDYAFKASPYPVILSLENHCTLEQQRVMARHLHAILGPMLLNRPLDGVTNSLPSPEQLKGKILLKGKKLGGLLPPGGEGGPEATVVSDEDEAAEMEDEAVRSRVQHKPKEDKLRLAQELSDMVIYCKSVHFGGFSSPGTPGQAFYEMASFSENRALRLLQESGNGFVRHNVGHLSRIYPAGWRTDSSNYSPVEMWNGGCQIVALNFQTPGPEMDVYQGRFQDNGACGYVLKPAFLRDPNGTFNPRALAQGPWWARKRLNIRVISGQQLPKVNKNKNSIVDPKVTVEIHGVSRDVASRQTAVITNNGFNPWWDTEFAFEVVVPDLALIRFLVEDYDASSKNDFIGQSTIPLNSLKQGYRHVHLMSKNGDQHPSATLFVKISLQD

Target ORF information:

RefSeq Version	NM_006225.4
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C delta 1 (PLCD1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006225.4

ORF Insert Sequence:

ATGGACTCGG GCCGGGACTT CCTGACCCTG CACGGCCTAC AGGATGATGA GGATCTACAG 
GCGCTGCTGA AGGGCAGCCA GCTCCTGAAG GTGAAGTCCA GCTCATGGAG GAGAGAGCGC 
TTCTACAAGT TGCAGGAGGA CTGCAAGACC ATCTGGCAGG AGTCCCGCAA GGTCATGCGG 
ACCCCGGAGT CCCAGCTGTT CTCCATCGAG GACATTCAGG AGGTGCGAAT GGGGCACCGC 
ACGGAGGGTC TGGAGAAGTT CGCCCGTGAT GTGCCCGAGG ACCGCTGCTT CTCCATTGTC 
TTCAAGGACC AGCGCAATAC ACTAGACCTC ATCGCCCCAT CGCCAGCTGA TGCCCAGCAC 
TGGGTGCTGG GGCTGCACAA GATCATCCAC CACTCAGGCT CCATGGACCA GCGTCAGAAG 
CTACAGCACT GGATTCACTC CTGCTTGCGA AAAGCTGACA AAAACAAGGA CAACAAGATG 
AGCTTCAAGG AGCTGCAGAA CTTCCTGAAG GAGCTCAACA TCCAGGTGGA CGACAGCTAT 
GCCCGGAAGA TCTTCAGGGA GTGTGACCAC TCCCAGACAG ACTCCCTGGA GGACGAGGAG 
ATTGAGGCCT TCTACAAGAT GCTGACCCAG CGGGTGGAGA TCGACCGCAC CTTCGCCGAG 
GCCGCGGGCT CAGGGGAGAC TCTGTCGGTG GATCAGTTAG TGACGTTCCT GCAGCACCAG 
CAGCGGGAGG AGGCGGCAGG GCCTGCGCTG GCCCTCTCCC TCATTGAGCG CTACGAGCCC 
AGCGAGACTG CCAAGGCGCA GCGGCAGATG ACCAAGGACG GCTTCCTCAT GTACTTACTG 
TCGGCTGACG GCAGCGCCTT CAGCCTGGCA CACCGCCGTG TCTACCAGGA CATGGGCCAG 
CCACTTAGCC ACTACCTGGT GTCCTCTTCA CACAACACCT ACCTGCTGGA GGACCAGCTA 
GCCGGGCCCA GCAGCACTGA AGCCTACATC CGGGCACTGT GCAAAGGCTG CCGATGCCTG 
GAGCTTGACT GCTGGGACGG GCCCAACCAG GAACCAATCA TCTACCACGG CTATACTTTC 
ACTTCCAAGA TCCTCTTCTG CGATGTGCTC AGGGCCATCC GGGACTATGC CTTCAAGGCG 
TCCCCCTACC CTGTCATCCT ATCCCTGGAG AACCACTGCA CACTGGAGCA GCAGCGCGTG 
ATGGCGCGGC ACCTGCATGC CATCCTGGGC CCCATGCTGT TGAACCGACC ACTGGATGGG 
GTCACCAACA GCCTGCCCTC CCCTGAGCAA CTGAAGGGGA AGATCCTGCT GAAGGGGAAG 
AAGCTCGGGG GGCTCCTGCC CCCTGGAGGG GAGGGTGGCC CTGAGGCCAC TGTGGTGTCA 
GACGAAGACG AGGCTGCTGA GATGGAGGAT GAGGCAGTGA GGAGCCGTGT GCAGCACAAG 
CCCAAGGAGG ACAAGCTCAG GCTAGCACAG GAGCTCTCTG ACATGGTCAT TTACTGCAAG 
AGTGTCCACT TTGGGGGCTT CTCCAGTCCT GGCACCCCTG GACAGGCCTT CTACGAGATG 
GCGTCCTTCT CTGAGAACCG TGCCCTTCGA CTGCTCCAAG AATCAGGAAA CGGCTTTGTC 
CGCCACAACG TGGGGCACCT GAGCAGAATC TACCCGGCTG GATGGAGAAC AGACTCCTCC 
AACTACAGCC CCGTGGAGAT GTGGAATGGG GGCTGCCAGA TCGTGGCCCT GAATTTCCAG 
ACACCTGGGC CAGAGATGGA CGTGTACCAG GGCCGCTTCC AGGACAACGG GGCCTGTGGG 
TACGTGCTGA AGCCCGCCTT CCTGCGAGAC CCCAACGGCA CCTTTAACCC CCGCGCCCTG 
GCTCAGGGGC CCTGGTGGGC ACGGAAGCGG CTCAACATCA GGGTCATTTC GGGGCAGCAG 
CTGCCAAAAG TCAACAAGAA TAAGAATTCA ATTGTGGACC CCAAAGTGAC AGTGGAGATC 
CATGGCGTGA GCCGGGACGT GGCCAGCCGC CAGACTGCTG TCATCACCAA CAATGGTTTC 
AACCCATGGT GGGACACGGA GTTTGCGTTT GAGGTAGTTG TGCCTGACCT TGCCCTCATC 
CGCTTCTTGG TGGAAGATTA TGATGCCTCC TCCAAGAATG ACTTCATTGG CCAGAGTACC 
ATCCCCTTGA ACAGCCTCAA GCAAGGATAC CGCCATGTCC ACCTCATGTC TAAGAACGGG 
GACCAGCATC CATCAGCCAC CCTCTTTGTG AAGATCTCCC TCCAGGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCD1 ( NM_001130964.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124436.1 Homo sapiens phospholipase C delta 1 (PLCD1), transcript variant 1, mRNA.

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CloneID	OHu26435
Clone ID Related Accession (Same CDS sequence)	NM_001130964.2 , NM_001130964.1
Accession Version	NM_001130964.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA083758.1, AK090774.1 and BC050382.1. On Jun 2, 2019 this sequence version replaced NM_001130964.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.973607.1, SRR7346977.968505.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGTGCC TGGGGATCCG GAGCCGGAGC CGCTCCAGGG AGCTCTACCT GCAGGAGCGG 
AGCCTTAAGG TGGCGGCGCT CAATGGACGG AGGCTGGGCC TACAGGATGA TGAGGATCTA 
CAGGCGCTGC TGAAGGGCAG CCAGCTCCTG AAGGTGAAGT CCAGCTCATG GAGGAGAGAG 
CGCTTCTACA AGTTGCAGGA GGACTGCAAG ACCATCTGGC AGGAGTCCCG CAAGGTCATG 
CGGACCCCGG AGTCCCAGCT GTTCTCCATC GAGGACATTC AGGAGGTGCG AATGGGGCAC 
CGCACGGAGG GTCTGGAGAA GTTCGCCCGT GATGTGCCCG AGGACCGCTG CTTCTCCATT 
GTCTTCAAGG ACCAGCGCAA TACACTAGAC CTCATCGCCC CATCGCCAGC TGATGCCCAG 
CACTGGGTGC TGGGGCTGCA CAAGATCATC CACCACTCAG GCTCCATGGA CCAGCGTCAG 
AAGCTACAGC ACTGGATTCA CTCCTGCTTG CGAAAAGCTG ACAAAAACAA GGACAACAAG 
ATGAGCTTCA AGGAGCTGCA GAACTTCCTG AAGGAGCTCA ACATCCAGGT GGACGACAGC 
TATGCCCGGA AGATCTTCAG GGAGTGTGAC CACTCCCAGA CAGACTCCCT GGAGGACGAG 
GAGATTGAGG CCTTCTACAA GATGCTGACC CAGCGGGTGG AGATCGACCG CACCTTCGCC 
GAGGCCGCGG GCTCAGGGGA GACTCTGTCG GTGGATCAGT TAGTGACGTT CCTGCAGCAC 
CAGCAGCGGG AGGAGGCGGC AGGGCCTGCG CTGGCCCTCT CCCTCATTGA GCGCTACGAG 
CCCAGCGAGA CTGCCAAGGC GCAGCGGCAG ATGACCAAGG ACGGCTTCCT CATGTACTTA 
CTGTCGGCTG ACGGCAGCGC CTTCAGCCTG GCACACCGCC GTGTCTACCA GGACATGGGC 
CAGCCACTTA GCCACTACCT GGTGTCCTCT TCACACAACA CCTACCTGCT GGAGGACCAG 
CTAGCCGGGC CCAGCAGCAC TGAAGCCTAC ATCCGGGCAC TGTGCAAAGG CTGCCGATGC 
CTGGAGCTTG ACTGCTGGGA CGGGCCCAAC CAGGAACCAA TCATCTACCA CGGCTATACT 
TTCACTTCCA AGATCCTCTT CTGCGATGTG CTCAGGGCCA TCCGGGACTA TGCCTTCAAG 
GCGTCCCCCT ACCCTGTCAT CCTATCCCTG GAGAACCACT GCACACTGGA GCAGCAGCGC 
GTGATGGCGC GGCACCTGCA TGCCATCCTG GGCCCCATGC TGTTGAACCG ACCACTGGAT 
GGGGTCACCA ACAGCCTGCC CTCCCCTGAG CAACTGAAGG GGAAGATCCT GCTGAAGGGG 
AAGAAGCTCG GGGGGCTCCT GCCCCCTGGA GGGGAGGGTG GCCCTGAGGC CACTGTGGTG 
TCAGACGAAG ACGAGGCTGC TGAGATGGAG GATGAGGCAG TGAGGAGCCG TGTGCAGCAC 
AAGCCCAAGG AGGACAAGCT CAGGCTAGCA CAGGAGCTCT CTGACATGGT CATTTACTGC 
AAGAGTGTCC ACTTTGGGGG CTTCTCCAGT CCTGGCACCC CTGGACAGGC CTTCTACGAG 
ATGGCGTCCT TCTCTGAGAA CCGTGCCCTT CGACTGCTCC AAGAATCAGG AAACGGCTTT 
GTCCGCCACA ACGTGGGGCA CCTGAGCAGA ATCTACCCGG CTGGATGGAG AACAGACTCC 
TCCAACTACA GCCCCGTGGA GATGTGGAAT GGGGGCTGCC AGATCGTGGC CCTGAATTTC 
CAGACACCTG GGCCAGAGAT GGACGTGTAC CAGGGCCGCT TCCAGGACAA CGGGGCCTGT 
GGGTACGTGC TGAAGCCCGC CTTCCTGCGA GACCCCAACG GCACCTTTAA CCCCCGCGCC 
CTGGCTCAGG GGCCCTGGTG GGCACGGAAG CGGCTCAACA TCAGGGTCAT TTCGGGGCAG 
CAGCTGCCAA AAGTCAACAA GAATAAGAAT TCAATTGTGG ACCCCAAAGT GACAGTGGAG 
ATCCATGGCG TGAGCCGGGA CGTGGCCAGC CGCCAGACTG CTGTCATCAC CAACAATGGT 
TTCAACCCAT GGTGGGACAC GGAGTTTGCG TTTGAGGTAG TTGTGCCTGA CCTTGCCCTC 
ATCCGCTTCT TGGTGGAAGA TTATGATGCC TCCTCCAAGA ATGACTTCAT TGGCCAGAGT 
ACCATCCCCT TGAACAGCCT CAAGCAAGGA TACCGCCATG TCCACCTCAT GTCTAAGAAC 
GGGGACCAGC ATCCATCAGC CACCCTCTTT GTGAAGATCT CCCTCCAGGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124436.1
CDS	88..2421
Translation	MQCLGIRSRSRSRELYLQERSLKVAALNGRRLGLQDDEDLQALLKGSQLLKVKSSSWRRERFYKLQEDCKTIWQESRKVMRTPESQLFSIEDIQEVRMGHRTEGLEKFARDVPEDRCFSIVFKDQRNTLDLIAPSPADAQHWVLGLHKIIHHSGSMDQRQKLQHWIHSCLRKADKNKDNKMSFKELQNFLKELNIQVDDSYARKIFRECDHSQTDSLEDEEIEAFYKMLTQRVEIDRTFAEAAGSGETLSVDQLVTFLQHQQREEAAGPALALSLIERYEPSETAKAQRQMTKDGFLMYLLSADGSAFSLAHRRVYQDMGQPLSHYLVSSSHNTYLLEDQLAGPSSTEAYIRALCKGCRCLELDCWDGPNQEPIIYHGYTFTSKILFCDVLRAIRDYAFKASPYPVILSLENHCTLEQQRVMARHLHAILGPMLLNRPLDGVTNSLPSPEQLKGKILLKGKKLGGLLPPGGEGGPEATVVSDEDEAAEMEDEAVRSRVQHKPKEDKLRLAQELSDMVIYCKSVHFGGFSSPGTPGQAFYEMASFSENRALRLLQESGNGFVRHNVGHLSRIYPAGWRTDSSNYSPVEMWNGGCQIVALNFQTPGPEMDVYQGRFQDNGACGYVLKPAFLRDPNGTFNPRALAQGPWWARKRLNIRVISGQQLPKVNKNKNSIVDPKVTVEIHGVSRDVASRQTAVITNNGFNPWWDTEFAFEVVVPDLALIRFLVEDYDASSKNDFIGQSTIPLNSLKQGYRHVHLMSKNGDQHPSATLFVKISLQD

Target ORF information:

RefSeq Version	NM_001130964.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C delta 1 (PLCD1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130964.2

ORF Insert Sequence:

ATGCAGTGCC TGGGGATCCG GAGCCGGAGC CGCTCCAGGG AGCTCTACCT GCAGGAGCGG 
AGCCTTAAGG TGGCGGCGCT CAATGGACGG AGGCTGGGCC TACAGGATGA TGAGGATCTA 
CAGGCGCTGC TGAAGGGCAG CCAGCTCCTG AAGGTGAAGT CCAGCTCATG GAGGAGAGAG 
CGCTTCTACA AGTTGCAGGA GGACTGCAAG ACCATCTGGC AGGAGTCCCG CAAGGTCATG 
CGGACCCCGG AGTCCCAGCT GTTCTCCATC GAGGACATTC AGGAGGTGCG AATGGGGCAC 
CGCACGGAGG GTCTGGAGAA GTTCGCCCGT GATGTGCCCG AGGACCGCTG CTTCTCCATT 
GTCTTCAAGG ACCAGCGCAA TACACTAGAC CTCATCGCCC CATCGCCAGC TGATGCCCAG 
CACTGGGTGC TGGGGCTGCA CAAGATCATC CACCACTCAG GCTCCATGGA CCAGCGTCAG 
AAGCTACAGC ACTGGATTCA CTCCTGCTTG CGAAAAGCTG ACAAAAACAA GGACAACAAG 
ATGAGCTTCA AGGAGCTGCA GAACTTCCTG AAGGAGCTCA ACATCCAGGT GGACGACAGC 
TATGCCCGGA AGATCTTCAG GGAGTGTGAC CACTCCCAGA CAGACTCCCT GGAGGACGAG 
GAGATTGAGG CCTTCTACAA GATGCTGACC CAGCGGGTGG AGATCGACCG CACCTTCGCC 
GAGGCCGCGG GCTCAGGGGA GACTCTGTCG GTGGATCAGT TAGTGACGTT CCTGCAGCAC 
CAGCAGCGGG AGGAGGCGGC AGGGCCTGCG CTGGCCCTCT CCCTCATTGA GCGCTACGAG 
CCCAGCGAGA CTGCCAAGGC GCAGCGGCAG ATGACCAAGG ACGGCTTCCT CATGTACTTA 
CTGTCGGCTG ACGGCAGCGC CTTCAGCCTG GCACACCGCC GTGTCTACCA GGACATGGGC 
CAGCCACTTA GCCACTACCT GGTGTCCTCT TCACACAACA CCTACCTGCT GGAGGACCAG 
CTAGCCGGGC CCAGCAGCAC TGAAGCCTAC ATCCGGGCAC TGTGCAAAGG CTGCCGATGC 
CTGGAGCTTG ACTGCTGGGA CGGGCCCAAC CAGGAACCAA TCATCTACCA CGGCTATACT 
TTCACTTCCA AGATCCTCTT CTGCGATGTG CTCAGGGCCA TCCGGGACTA TGCCTTCAAG 
GCGTCCCCCT ACCCTGTCAT CCTATCCCTG GAGAACCACT GCACACTGGA GCAGCAGCGC 
GTGATGGCGC GGCACCTGCA TGCCATCCTG GGCCCCATGC TGTTGAACCG ACCACTGGAT 
GGGGTCACCA ACAGCCTGCC CTCCCCTGAG CAACTGAAGG GGAAGATCCT GCTGAAGGGG 
AAGAAGCTCG GGGGGCTCCT GCCCCCTGGA GGGGAGGGTG GCCCTGAGGC CACTGTGGTG 
TCAGACGAAG ACGAGGCTGC TGAGATGGAG GATGAGGCAG TGAGGAGCCG TGTGCAGCAC 
AAGCCCAAGG AGGACAAGCT CAGGCTAGCA CAGGAGCTCT CTGACATGGT CATTTACTGC 
AAGAGTGTCC ACTTTGGGGG CTTCTCCAGT CCTGGCACCC CTGGACAGGC CTTCTACGAG 
ATGGCGTCCT TCTCTGAGAA CCGTGCCCTT CGACTGCTCC AAGAATCAGG AAACGGCTTT 
GTCCGCCACA ACGTGGGGCA CCTGAGCAGA ATCTACCCGG CTGGATGGAG AACAGACTCC 
TCCAACTACA GCCCCGTGGA GATGTGGAAT GGGGGCTGCC AGATCGTGGC CCTGAATTTC 
CAGACACCTG GGCCAGAGAT GGACGTGTAC CAGGGCCGCT TCCAGGACAA CGGGGCCTGT 
GGGTACGTGC TGAAGCCCGC CTTCCTGCGA GACCCCAACG GCACCTTTAA CCCCCGCGCC 
CTGGCTCAGG GGCCCTGGTG GGCACGGAAG CGGCTCAACA TCAGGGTCAT TTCGGGGCAG 
CAGCTGCCAA AAGTCAACAA GAATAAGAAT TCAATTGTGG ACCCCAAAGT GACAGTGGAG 
ATCCATGGCG TGAGCCGGGA CGTGGCCAGC CGCCAGACTG CTGTCATCAC CAACAATGGT 
TTCAACCCAT GGTGGGACAC GGAGTTTGCG TTTGAGGTAG TTGTGCCTGA CCTTGCCCTC 
ATCCGCTTCT TGGTGGAAGA TTATGATGCC TCCTCCAAGA ATGACTTCAT TGGCCAGAGT 
ACCATCCCCT TGAACAGCCT CAAGCAAGGA TACCGCCATG TCCACCTCAT GTCTAAGAAC 
GGGGACCAGC ATCCATCAGC CACCCTCTTT GTGAAGATCT CCCTCCAGGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCD1 ( NM_001130964.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124436.1 Homo sapiens phospholipase C delta 1 (PLCD1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26435
Clone ID Related Accession (Same CDS sequence)	NM_001130964.2 , NM_001130964.1
Accession Version	NM_001130964.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-08
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA083758.1, AK090774.1 and BC050382.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.128852.1, SRR1803612.6058.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGTGCC TGGGGATCCG GAGCCGGAGC CGCTCCAGGG AGCTCTACCT GCAGGAGCGG 
AGCCTTAAGG TGGCGGCGCT CAATGGACGG AGGCTGGGCC TACAGGATGA TGAGGATCTA 
CAGGCGCTGC TGAAGGGCAG CCAGCTCCTG AAGGTGAAGT CCAGCTCATG GAGGAGAGAG 
CGCTTCTACA AGTTGCAGGA GGACTGCAAG ACCATCTGGC AGGAGTCCCG CAAGGTCATG 
CGGACCCCGG AGTCCCAGCT GTTCTCCATC GAGGACATTC AGGAGGTGCG AATGGGGCAC 
CGCACGGAGG GTCTGGAGAA GTTCGCCCGT GATGTGCCCG AGGACCGCTG CTTCTCCATT 
GTCTTCAAGG ACCAGCGCAA TACACTAGAC CTCATCGCCC CATCGCCAGC TGATGCCCAG 
CACTGGGTGC TGGGGCTGCA CAAGATCATC CACCACTCAG GCTCCATGGA CCAGCGTCAG 
AAGCTACAGC ACTGGATTCA CTCCTGCTTG CGAAAAGCTG ACAAAAACAA GGACAACAAG 
ATGAGCTTCA AGGAGCTGCA GAACTTCCTG AAGGAGCTCA ACATCCAGGT GGACGACAGC 
TATGCCCGGA AGATCTTCAG GGAGTGTGAC CACTCCCAGA CAGACTCCCT GGAGGACGAG 
GAGATTGAGG CCTTCTACAA GATGCTGACC CAGCGGGTGG AGATCGACCG CACCTTCGCC 
GAGGCCGCGG GCTCAGGGGA GACTCTGTCG GTGGATCAGT TAGTGACGTT CCTGCAGCAC 
CAGCAGCGGG AGGAGGCGGC AGGGCCTGCG CTGGCCCTCT CCCTCATTGA GCGCTACGAG 
CCCAGCGAGA CTGCCAAGGC GCAGCGGCAG ATGACCAAGG ACGGCTTCCT CATGTACTTA 
CTGTCGGCTG ACGGCAGCGC CTTCAGCCTG GCACACCGCC GTGTCTACCA GGACATGGGC 
CAGCCACTTA GCCACTACCT GGTGTCCTCT TCACACAACA CCTACCTGCT GGAGGACCAG 
CTAGCCGGGC CCAGCAGCAC TGAAGCCTAC ATCCGGGCAC TGTGCAAAGG CTGCCGATGC 
CTGGAGCTTG ACTGCTGGGA CGGGCCCAAC CAGGAACCAA TCATCTACCA CGGCTATACT 
TTCACTTCCA AGATCCTCTT CTGCGATGTG CTCAGGGCCA TCCGGGACTA TGCCTTCAAG 
GCGTCCCCCT ACCCTGTCAT CCTATCCCTG GAGAACCACT GCACACTGGA GCAGCAGCGC 
GTGATGGCGC GGCACCTGCA TGCCATCCTG GGCCCCATGC TGTTGAACCG ACCACTGGAT 
GGGGTCACCA ACAGCCTGCC CTCCCCTGAG CAACTGAAGG GGAAGATCCT GCTGAAGGGG 
AAGAAGCTCG GGGGGCTCCT GCCCCCTGGA GGGGAGGGTG GCCCTGAGGC CACTGTGGTG 
TCAGACGAAG ACGAGGCTGC TGAGATGGAG GATGAGGCAG TGAGGAGCCG TGTGCAGCAC 
AAGCCCAAGG AGGACAAGCT CAGGCTAGCA CAGGAGCTCT CTGACATGGT CATTTACTGC 
AAGAGTGTCC ACTTTGGGGG CTTCTCCAGT CCTGGCACCC CTGGACAGGC CTTCTACGAG 
ATGGCGTCCT TCTCTGAGAA CCGTGCCCTT CGACTGCTCC AAGAATCAGG AAACGGCTTT 
GTCCGCCACA ACGTGGGGCA CCTGAGCAGA ATCTACCCGG CTGGATGGAG AACAGACTCC 
TCCAACTACA GCCCCGTGGA GATGTGGAAT GGGGGCTGCC AGATCGTGGC CCTGAATTTC 
CAGACACCTG GGCCAGAGAT GGACGTGTAC CAGGGCCGCT TCCAGGACAA CGGGGCCTGT 
GGGTACGTGC TGAAGCCCGC CTTCCTGCGA GACCCCAACG GCACCTTTAA CCCCCGCGCC 
CTGGCTCAGG GGCCCTGGTG GGCACGGAAG CGGCTCAACA TCAGGGTCAT TTCGGGGCAG 
CAGCTGCCAA AAGTCAACAA GAATAAGAAT TCAATTGTGG ACCCCAAAGT GACAGTGGAG 
ATCCATGGCG TGAGCCGGGA CGTGGCCAGC CGCCAGACTG CTGTCATCAC CAACAATGGT 
TTCAACCCAT GGTGGGACAC GGAGTTTGCG TTTGAGGTAG TTGTGCCTGA CCTTGCCCTC 
ATCCGCTTCT TGGTGGAAGA TTATGATGCC TCCTCCAAGA ATGACTTCAT TGGCCAGAGT 
ACCATCCCCT TGAACAGCCT CAAGCAAGGA TACCGCCATG TCCACCTCAT GTCTAAGAAC 
GGGGACCAGC ATCCATCAGC CACCCTCTTT GTGAAGATCT CCCTCCAGGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124436.1
CDS	355..2688
Translation	MQCLGIRSRSRSRELYLQERSLKVAALNGRRLGLQDDEDLQALLKGSQLLKVKSSSWRRERFYKLQEDCKTIWQESRKVMRTPESQLFSIEDIQEVRMGHRTEGLEKFARDVPEDRCFSIVFKDQRNTLDLIAPSPADAQHWVLGLHKIIHHSGSMDQRQKLQHWIHSCLRKADKNKDNKMSFKELQNFLKELNIQVDDSYARKIFRECDHSQTDSLEDEEIEAFYKMLTQRVEIDRTFAEAAGSGETLSVDQLVTFLQHQQREEAAGPALALSLIERYEPSETAKAQRQMTKDGFLMYLLSADGSAFSLAHRRVYQDMGQPLSHYLVSSSHNTYLLEDQLAGPSSTEAYIRALCKGCRCLELDCWDGPNQEPIIYHGYTFTSKILFCDVLRAIRDYAFKASPYPVILSLENHCTLEQQRVMARHLHAILGPMLLNRPLDGVTNSLPSPEQLKGKILLKGKKLGGLLPPGGEGGPEATVVSDEDEAAEMEDEAVRSRVQHKPKEDKLRLAQELSDMVIYCKSVHFGGFSSPGTPGQAFYEMASFSENRALRLLQESGNGFVRHNVGHLSRIYPAGWRTDSSNYSPVEMWNGGCQIVALNFQTPGPEMDVYQGRFQDNGACGYVLKPAFLRDPNGTFNPRALAQGPWWARKRLNIRVISGQQLPKVNKNKNSIVDPKVTVEIHGVSRDVASRQTAVITNNGFNPWWDTEFAFEVVVPDLALIRFLVEDYDASSKNDFIGQSTIPLNSLKQGYRHVHLMSKNGDQHPSATLFVKISLQD

Target ORF information:

RefSeq Version	NM_001130964.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C delta 1 (PLCD1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130964.1

ORF Insert Sequence:

ATGCAGTGCC TGGGGATCCG GAGCCGGAGC CGCTCCAGGG AGCTCTACCT GCAGGAGCGG 
AGCCTTAAGG TGGCGGCGCT CAATGGACGG AGGCTGGGCC TACAGGATGA TGAGGATCTA 
CAGGCGCTGC TGAAGGGCAG CCAGCTCCTG AAGGTGAAGT CCAGCTCATG GAGGAGAGAG 
CGCTTCTACA AGTTGCAGGA GGACTGCAAG ACCATCTGGC AGGAGTCCCG CAAGGTCATG 
CGGACCCCGG AGTCCCAGCT GTTCTCCATC GAGGACATTC AGGAGGTGCG AATGGGGCAC 
CGCACGGAGG GTCTGGAGAA GTTCGCCCGT GATGTGCCCG AGGACCGCTG CTTCTCCATT 
GTCTTCAAGG ACCAGCGCAA TACACTAGAC CTCATCGCCC CATCGCCAGC TGATGCCCAG 
CACTGGGTGC TGGGGCTGCA CAAGATCATC CACCACTCAG GCTCCATGGA CCAGCGTCAG 
AAGCTACAGC ACTGGATTCA CTCCTGCTTG CGAAAAGCTG ACAAAAACAA GGACAACAAG 
ATGAGCTTCA AGGAGCTGCA GAACTTCCTG AAGGAGCTCA ACATCCAGGT GGACGACAGC 
TATGCCCGGA AGATCTTCAG GGAGTGTGAC CACTCCCAGA CAGACTCCCT GGAGGACGAG 
GAGATTGAGG CCTTCTACAA GATGCTGACC CAGCGGGTGG AGATCGACCG CACCTTCGCC 
GAGGCCGCGG GCTCAGGGGA GACTCTGTCG GTGGATCAGT TAGTGACGTT CCTGCAGCAC 
CAGCAGCGGG AGGAGGCGGC AGGGCCTGCG CTGGCCCTCT CCCTCATTGA GCGCTACGAG 
CCCAGCGAGA CTGCCAAGGC GCAGCGGCAG ATGACCAAGG ACGGCTTCCT CATGTACTTA 
CTGTCGGCTG ACGGCAGCGC CTTCAGCCTG GCACACCGCC GTGTCTACCA GGACATGGGC 
CAGCCACTTA GCCACTACCT GGTGTCCTCT TCACACAACA CCTACCTGCT GGAGGACCAG 
CTAGCCGGGC CCAGCAGCAC TGAAGCCTAC ATCCGGGCAC TGTGCAAAGG CTGCCGATGC 
CTGGAGCTTG ACTGCTGGGA CGGGCCCAAC CAGGAACCAA TCATCTACCA CGGCTATACT 
TTCACTTCCA AGATCCTCTT CTGCGATGTG CTCAGGGCCA TCCGGGACTA TGCCTTCAAG 
GCGTCCCCCT ACCCTGTCAT CCTATCCCTG GAGAACCACT GCACACTGGA GCAGCAGCGC 
GTGATGGCGC GGCACCTGCA TGCCATCCTG GGCCCCATGC TGTTGAACCG ACCACTGGAT 
GGGGTCACCA ACAGCCTGCC CTCCCCTGAG CAACTGAAGG GGAAGATCCT GCTGAAGGGG 
AAGAAGCTCG GGGGGCTCCT GCCCCCTGGA GGGGAGGGTG GCCCTGAGGC CACTGTGGTG 
TCAGACGAAG ACGAGGCTGC TGAGATGGAG GATGAGGCAG TGAGGAGCCG TGTGCAGCAC 
AAGCCCAAGG AGGACAAGCT CAGGCTAGCA CAGGAGCTCT CTGACATGGT CATTTACTGC 
AAGAGTGTCC ACTTTGGGGG CTTCTCCAGT CCTGGCACCC CTGGACAGGC CTTCTACGAG 
ATGGCGTCCT TCTCTGAGAA CCGTGCCCTT CGACTGCTCC AAGAATCAGG AAACGGCTTT 
GTCCGCCACA ACGTGGGGCA CCTGAGCAGA ATCTACCCGG CTGGATGGAG AACAGACTCC 
TCCAACTACA GCCCCGTGGA GATGTGGAAT GGGGGCTGCC AGATCGTGGC CCTGAATTTC 
CAGACACCTG GGCCAGAGAT GGACGTGTAC CAGGGCCGCT TCCAGGACAA CGGGGCCTGT 
GGGTACGTGC TGAAGCCCGC CTTCCTGCGA GACCCCAACG GCACCTTTAA CCCCCGCGCC 
CTGGCTCAGG GGCCCTGGTG GGCACGGAAG CGGCTCAACA TCAGGGTCAT TTCGGGGCAG 
CAGCTGCCAA AAGTCAACAA GAATAAGAAT TCAATTGTGG ACCCCAAAGT GACAGTGGAG 
ATCCATGGCG TGAGCCGGGA CGTGGCCAGC CGCCAGACTG CTGTCATCAC CAACAATGGT 
TTCAACCCAT GGTGGGACAC GGAGTTTGCG TTTGAGGTAG TTGTGCCTGA CCTTGCCCTC 
ATCCGCTTCT TGGTGGAAGA TTATGATGCC TCCTCCAAGA ATGACTTCAT TGGCCAGAGT 
ACCATCCCCT TGAACAGCCT CAAGCAAGGA TACCGCCATG TCCACCTCAT GTCTAAGAAC 
GGGGACCAGC ATCCATCAGC CACCCTCTTT GTGAAGATCT CCCTCCAGGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCD1 ( XM_017006622.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862111.1 Homo sapiens phospholipase C delta 1 (PLCD1), transcript variant X1, mRNA.

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CloneID	OHu94289
Clone ID Related Accession (Same CDS sequence)	XM_017006622.1
Accession Version	XM_017006622.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGACCC CGGAGTCCCA GCTGTTCTCC ATCGAGGACA TTCAGGAGGT GCGAATGGGG 
CACCGCACGG AGGGTCTGGA GAAGTTCGCC CGTGATGTGC CCGAGGACCG CTGCTTCTCC 
ATTGTCTTCA AGGACCAGCG CAATACACTA GACCTCATCG CCCCATCGCC AGCTGATGCC 
CAGCACTGGG TGCTGGGGCT GCACAAGATC ATCCACCACT CAGGCTCCAT GGACCAGCGT 
CAGAAGCTAC AGCACTGGAT TCACTCCTGC TTGCGAAAAG CTGACAAAAA CAAGGACAAC 
AAGATGAGCT TCAAGGAGCT GCAGAACTTC CTGAAGGAGC TCAACATCCA GGTGGACGAC 
AGCTATGCCC GGAAGATCTT CAGGGAGTGT GACCACTCCC AGACAGACTC CCTGGAGGAC 
GAGGAGATTG AGGCCTTCTA CAAGATGCTG ACCCAGCGGG TGGAGATCGA CCGCACCTTC 
GCCGAGGCCG CGGGCTCAGG GGAGACTCTG TCGGTGGATC AGTTAGTGAC GTTCCTGCAG 
CACCAGCAGC GGGAGGAGGC GGCAGGGCCT GCGCTGGCCC TCTCCCTCAT TGAGCGCTAC 
GAGCCCAGCG AGACTGCCAA GGCGCAGCGG CAGATGACCA AGGACGGCTT CCTCATGTAC 
TTACTGTCGG CTGACGGCAG CGCCTTCAGC CTGGCACACC GCCGTGTCTA CCAGGACATG 
GGCCAGCCAC TTAGCCACTA CCTGGTGTCC TCTTCACACA ACACCTACCT GCTGGAGGAC 
CAGCTAGCCG GGCCCAGCAG CACTGAAGCC TACATCCGGG CACTGTGCAA AGGCTGCCGA 
TGCCTGGAGC TTGACTGCTG GGACGGGCCC AACCAGGAAC CAATCATCTA CCACGGCTAT 
ACTTTCACTT CCAAGATCCT CTTCTGCGAT GTGCTCAGGG CCATCCGGGA CTATGCCTTC 
AAGGCGTCCC CCTACCCTGT CATCCTATCC CTGGAGAACC ACTGCACACT GGAGCAGCAG 
CGCGTGATGG CGCGGCACCT GCATGCCATC CTGGGCCCCA TGCTGTTGAA CCGACCACTG 
GATGGGGTCA CCAACAGCCT GCCCTCCCCT GAGCAACTGA AGGGGAAGAT CCTGCTGAAG 
GGGAAGAAGC TCGGGGGGCT CCTGCCCCCT GGAGGGGAGG GTGGCCCTGA GGCCACTGTG 
GTGTCAGACG AAGACGAGGC TGCTGAGATG GAGGATGAGG CAGTGAGGAG CCGTGTGCAG 
CACAAGCCCA AGGAGGACAA GCTCAGGCTA GCACAGGAGC TCTCTGACAT GGTCATTTAC 
TGCAAGAGTG TCCACTTTGG GGGCTTCTCC AGTCCTGGCA CCCCTGGACA GGCCTTCTAC 
GAGATGGCGT CCTTCTCTGA GAACCGTGCC CTTCGACTGC TCCAAGAATC AGGAAACGGC 
TTTGTCCGCC ACAACGTGGG GCACCTGAGC AGAATCTACC CGGCTGGATG GAGAACAGAC 
TCCTCCAACT ACAGCCCCGT GGAGATGTGG AATGGGGGCT GCCAGATCGT GGCCCTGAAT 
TTCCAGACAC CTGGGCCAGA GATGGACGTG TACCAGGGCC GCTTCCAGGA CAACGGGGCC 
TGTGGGTACG TGCTGAAGCC CGCCTTCCTG CGAGACCCCA ACGGCACCTT TAACCCCCGC 
GCCCTGGCTC AGGGGCCCTG GTGGGCACGG AAGCGGCTCA ACATCAGGGT CATTTCGGGG 
CAGCAGCTGC CAAAAGTCAA CAAGAATAAG AATTCAATTG TGGACCCCAA AGTGACAGTG 
GAGATCCATG GCGTGAGCCG GGACGTGGCC AGCCGCCAGA CTGCTGTCAT CACCAACAAT 
GGTTTCAACC CATGGTGGGA CACGGAGTTT GCGTTTGAGG TAGTTGTGCC TGACCTTGCC 
CTCATCCGCT TCTTGGTGGA AGATTATGAT GCCTCCTCCA AGAATGACTT CATTGGCCAG 
AGTACCATCC CCTTGAACAG CCTCAAGCAA GGATACCGCC ATGTCCACCT CATGTCTAAG 
AACGGGGACC AGCATCCATC AGCCACCCTC TTTGTGAAGA TCTCCCTCCA GGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862111.1
CDS	562..2658
Translation	MRTPESQLFSIEDIQEVRMGHRTEGLEKFARDVPEDRCFSIVFKDQRNTLDLIAPSPADAQHWVLGLHKIIHHSGSMDQRQKLQHWIHSCLRKADKNKDNKMSFKELQNFLKELNIQVDDSYARKIFRECDHSQTDSLEDEEIEAFYKMLTQRVEIDRTFAEAAGSGETLSVDQLVTFLQHQQREEAAGPALALSLIERYEPSETAKAQRQMTKDGFLMYLLSADGSAFSLAHRRVYQDMGQPLSHYLVSSSHNTYLLEDQLAGPSSTEAYIRALCKGCRCLELDCWDGPNQEPIIYHGYTFTSKILFCDVLRAIRDYAFKASPYPVILSLENHCTLEQQRVMARHLHAILGPMLLNRPLDGVTNSLPSPEQLKGKILLKGKKLGGLLPPGGEGGPEATVVSDEDEAAEMEDEAVRSRVQHKPKEDKLRLAQELSDMVIYCKSVHFGGFSSPGTPGQAFYEMASFSENRALRLLQESGNGFVRHNVGHLSRIYPAGWRTDSSNYSPVEMWNGGCQIVALNFQTPGPEMDVYQGRFQDNGACGYVLKPAFLRDPNGTFNPRALAQGPWWARKRLNIRVISGQQLPKVNKNKNSIVDPKVTVEIHGVSRDVASRQTAVITNNGFNPWWDTEFAFEVVVPDLALIRFLVEDYDASSKNDFIGQSTIPLNSLKQGYRHVHLMSKNGDQHPSATLFVKISLQD

Target ORF information:

RefSeq Version	XM_017006622.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C delta 1 (PLCD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017006622.1

ORF Insert Sequence:

ATGCGGACCC CGGAGTCCCA GCTGTTCTCC ATCGAGGACA TTCAGGAGGT GCGAATGGGG 
CACCGCACGG AGGGTCTGGA GAAGTTCGCC CGTGATGTGC CCGAGGACCG CTGCTTCTCC 
ATTGTCTTCA AGGACCAGCG CAATACACTA GACCTCATCG CCCCATCGCC AGCTGATGCC 
CAGCACTGGG TGCTGGGGCT GCACAAGATC ATCCACCACT CAGGCTCCAT GGACCAGCGT 
CAGAAGCTAC AGCACTGGAT TCACTCCTGC TTGCGAAAAG CTGACAAAAA CAAGGACAAC 
AAGATGAGCT TCAAGGAGCT GCAGAACTTC CTGAAGGAGC TCAACATCCA GGTGGACGAC 
AGCTATGCCC GGAAGATCTT CAGGGAGTGT GACCACTCCC AGACAGACTC CCTGGAGGAC 
GAGGAGATTG AGGCCTTCTA CAAGATGCTG ACCCAGCGGG TGGAGATCGA CCGCACCTTC 
GCCGAGGCCG CGGGCTCAGG GGAGACTCTG TCGGTGGATC AGTTAGTGAC GTTCCTGCAG 
CACCAGCAGC GGGAGGAGGC GGCAGGGCCT GCGCTGGCCC TCTCCCTCAT TGAGCGCTAC 
GAGCCCAGCG AGACTGCCAA GGCGCAGCGG CAGATGACCA AGGACGGCTT CCTCATGTAC 
TTACTGTCGG CTGACGGCAG CGCCTTCAGC CTGGCACACC GCCGTGTCTA CCAGGACATG 
GGCCAGCCAC TTAGCCACTA CCTGGTGTCC TCTTCACACA ACACCTACCT GCTGGAGGAC 
CAGCTAGCCG GGCCCAGCAG CACTGAAGCC TACATCCGGG CACTGTGCAA AGGCTGCCGA 
TGCCTGGAGC TTGACTGCTG GGACGGGCCC AACCAGGAAC CAATCATCTA CCACGGCTAT 
ACTTTCACTT CCAAGATCCT CTTCTGCGAT GTGCTCAGGG CCATCCGGGA CTATGCCTTC 
AAGGCGTCCC CCTACCCTGT CATCCTATCC CTGGAGAACC ACTGCACACT GGAGCAGCAG 
CGCGTGATGG CGCGGCACCT GCATGCCATC CTGGGCCCCA TGCTGTTGAA CCGACCACTG 
GATGGGGTCA CCAACAGCCT GCCCTCCCCT GAGCAACTGA AGGGGAAGAT CCTGCTGAAG 
GGGAAGAAGC TCGGGGGGCT CCTGCCCCCT GGAGGGGAGG GTGGCCCTGA GGCCACTGTG 
GTGTCAGACG AAGACGAGGC TGCTGAGATG GAGGATGAGG CAGTGAGGAG CCGTGTGCAG 
CACAAGCCCA AGGAGGACAA GCTCAGGCTA GCACAGGAGC TCTCTGACAT GGTCATTTAC 
TGCAAGAGTG TCCACTTTGG GGGCTTCTCC AGTCCTGGCA CCCCTGGACA GGCCTTCTAC 
GAGATGGCGT CCTTCTCTGA GAACCGTGCC CTTCGACTGC TCCAAGAATC AGGAAACGGC 
TTTGTCCGCC ACAACGTGGG GCACCTGAGC AGAATCTACC CGGCTGGATG GAGAACAGAC 
TCCTCCAACT ACAGCCCCGT GGAGATGTGG AATGGGGGCT GCCAGATCGT GGCCCTGAAT 
TTCCAGACAC CTGGGCCAGA GATGGACGTG TACCAGGGCC GCTTCCAGGA CAACGGGGCC 
TGTGGGTACG TGCTGAAGCC CGCCTTCCTG CGAGACCCCA ACGGCACCTT TAACCCCCGC 
GCCCTGGCTC AGGGGCCCTG GTGGGCACGG AAGCGGCTCA ACATCAGGGT CATTTCGGGG 
CAGCAGCTGC CAAAAGTCAA CAAGAATAAG AATTCAATTG TGGACCCCAA AGTGACAGTG 
GAGATCCATG GCGTGAGCCG GGACGTGGCC AGCCGCCAGA CTGCTGTCAT CACCAACAAT 
GGTTTCAACC CATGGTGGGA CACGGAGTTT GCGTTTGAGG TAGTTGTGCC TGACCTTGCC 
CTCATCCGCT TCTTGGTGGA AGATTATGAT GCCTCCTCCA AGAATGACTT CATTGGCCAG 
AGTACCATCC CCTTGAACAG CCTCAAGCAA GGATACCGCC ATGTCCACCT CATGTCTAAG 
AACGGGGACC AGCATCCATC AGCCACCCTC TTTGTGAAGA TCTCCCTCCA GGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mutations of candidate tumor suppressor genes at chromosome 3p in intrahepatic cholangiocarcinoma.
Experimental and molecular pathology103(3)249-254(2017 12)
You HL,Huang WT,Liu TT,Weng SW,Eng HL

Phospholipase C ?1 negatively regulates autophagy in colorectal cancer cells.
Biochemical and biophysical research communications488(4)578-583(2017 07)
Shimozawa M,Anzai S,Satow R,Fukami K

Phospholipase C?1 regulates p38 MAPK activity and skin barrier integrity.
Cell death and differentiation24(6)1079-1090(2017 Jun)
Kanemaru K,Nakamura Y,Totoki K,Fukuyama T,Shoji M,Kaneko H,Shiratori K,Yoneda A,Inoue T,Iwakura Y,Kabashima K,Fukami K

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PLCD1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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