Pitpnm1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pitpnm1
Entrez Gene ID	18739
Full Name	phosphatidylinositol transfer protein, membrane-associated 1
Synonyms	DRES9,Mpt-1,Nir-2,PITPnm 1,Pitpnm,R75447,Rd9,RdgB,RdgB1
General protein information	Preferred Names phosphatidylinositol transfer protein, membrane-associated 1 Names membrane-associated phosphatidylinositol transfer protein 1 drosophila retinal degeneration B homolog 1 pyk2 N-terminal domain-interacting receptor 2 retinal degeneration 9
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19 A\|19 3.81 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_017318081.1	XP_017173570.1	membrane-associated phosphatidylinositol transfer protein 1 isoform X1
XM_006531680.3	XP_006531743.1	membrane-associated phosphatidylinositol transfer protein 1 isoform X2
XM_006531680.4	XP_006531743.1	membrane-associated phosphatidylinositol transfer protein 1 isoform X1
NM_008851.4	NP_032877.1	membrane-associated phosphatidylinositol transfer protein 1
XM_030250773.1	XP_030106633.1	membrane-associated phosphatidylinositol transfer protein 1 isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Pitpnm1	NP_032877.1
Rattus norvegicus (Norway rat)	Pitpnm1	NP_001008370.1
Homo sapiens (human)	PITPNM1	NP_004901.2
Pan troglodytes (chimpanzee)	PITPNM1	XP_003313225.1
	PITPNM1	XP_001117918.1
Gallus gallus (chicken)	PITPNM1	XP_001233221.3
Canis lupus familiaris (dog)	PITPNM1	XP_851829.1
Bos taurus (cattle)	PITPNM1	XP_002699412.1

Gene Ontology
Bibliography

Related articles in PubMed

The lipid-transfer protein Nir2 enhances epithelial-mesenchymal transition and facilitates breast cancer metastasis.
Keinan O, Kedan A, Gavert N, Selitrennik M, Kim S, Karn T, Becker S, Lev S
Journal of cell science127(Pt 21)4740-9(2014 Nov)

Pitpnm1 is expressed in hair cells during development but is not required for hearing.
Carlisle FA, Pearson S, Steel KP, Lewis MA
Neuroscience248620-5(2013 Sep)

Ezh2, the histone methyltransferase of PRC2, regulates the balance between self-renewal and differentiation in the cerebral cortex.
Pereira JD, Sansom SN, Smith J, Dobenecker MW, Tarakhovsky A, Livesey FJ
Proceedings of the National Academy of Sciences of the United States of America107(36)15957-62(2010 Sep)

Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations.
Munton RP, Tweedie-Cullen R, Livingstone-Zatchej M, Weinandy F, Waidelich M, Longo D, Gehrig P, Potthast F, Rutishauser D, Gerrits B, Panse C, Schlapbach R, Mansuy IM
Molecular & cellular proteomics : MCP6(2)283-93(2007 Feb)

Substrate specificity of RdgB protein, a deoxyribonucleoside triphosphate pyrophosphohydrolase.
Burgis NE, Cunningham RP
The Journal of biological chemistry282(6)3531-8(2007 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results suggest that Nir2 not only enhances EMT in vitro and breast cancer metastasis in animal models, but also contributes to breast cancer progression in human patients.
Title: The lipid-transfer protein Nir2 enhances epithelial-mesenchymal transition and facilitates breast cancer metastasis.

Pitpnm1 null mice showed no hearing defects.
Title: Pitpnm1 is expressed in hair cells during development but is not required for hearing.

RdgB homologs play a preemptive role in excluding endogenous and exogenous modified purine deoxyribonucleoside triphosphates (dTNPs) from incorporation into DNA.
Title: Substrate specificity of RdgB protein, a deoxyribonucleoside triphosphate pyrophosphohydrolase.

The following Pitpnm1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pitpnm1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu55028	XM_006531680.4 Latest version!	Mus musculus phosphatidylinositol transfer protein, membrane-associated 1 (Pitpnm1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu87495	XM_030250773.1 Latest version!	Mus musculus phosphatidylinositol transfer protein, membrane-associated 1 (Pitpnm1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu18332	NM_008851.4 Latest version!	Mus musculus phosphatidylinositol transfer protein, membrane-associated 1 (Pitpnm1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$727.30 $1039.00
OMu79901	XM_017318081.1 Latest version!	Mus musculus phosphatidylinositol transfer protein, membrane-associated 1 (Pitpnm1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OMu55028	XM_006531680.3	Mus musculus phosphatidylinositol transfer protein, membrane-associated 1 (Pitpnm1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pitpnm1 ( XM_006531680.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531743.1 Mus musculus phosphatidylinositol transfer protein, membrane-associated 1 (Pitpnm1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu55028
Clone ID Related Accession (Same CDS sequence)	XM_006531680.3 , XM_006531680.4
Accession Version	XM_006531680.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	membrane-associated phosphatidylinositol transfer protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006531680.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTATCA AGGAGTACCA CATCCTGCTG CCCATGAGCC TGGACGAGTA TCAAGTGGCC 
CAGCTCTACA TGATCCAGAA AAAGAGCCGT GAGGAGTCTA GTGGTGAGGG CAGCGGCGTG 
GAGATCCTGG CCAACCGGCC CTACACAGAT GGGCCTGGAG GCAACGGGCA GTATACACAC 
AAGGTGTACC ACGTGGGCTC CCACATCCCA GGCTGGTTCC GGGCACTGTT ACCCAAGGCT 
GCTCTGCAGG TAGAAGAGGA ATCTTGGAAC GCTTACCCAT ATACCCGGAC ACGGTACACC 
TGCCCCTTTG TGGAGAAGTT CTCCATTGAG ATAGAGACCT ACTACTTGCC TGATGGGGGG 
CAGCAACCTA ACGTCTTCAA CCTGAGTGGG GCTGAGAGAA GACAGAGAAT CGTGGATACC 
ATCGACATCG TGCGGGATGC AGTGGCCCCA GGAGAATACA AAGCGGAAGA GGACCCTCGG 
CTGTACCGCT CAGCCAAGAC AGGCCGAGGG CCGCTGGCTG ATGACTGGGC ACGGACAGCG 
GCCCAGACAG GACCTCTCAT GTGTGCCTAT AAGCTGTGCA AGGTTGAATT CCGCTACTGG 
GGCATGCAGG CCAAGATTGA GCAGTTCATC CATGACGTAG GTCTGCGCAG GGTGATGCTT 
CGCGCCCATC GCCAGGCCTG GTGTTGGCAA GATGAGTGGA TAGAACTGAG CATGGCTGAC 
ATCCGGGCAC TGGAGGAGGA GACTGCACGC ATGCTAGCGC AGCGTATGGC TAAGTGCAAC 
ACTGGCAGTG AGGGACCAGA GGCTCAGACC CCTGGGAAAT CCAGCACTGA GGCCCGACCT 
GGGACCAGCA CTGCTGGCAC CCCTGATGGA CCTGAGGCCC CTCCCGGCCC CGACGCCTCC 
CCAGATGCCA GCTTTGGGAA GCAGTGGTCC TCATCCTCCC GTTCCTCCTA CTCATCCCAA 
CATGGAGGCG GCGTGTCTCC ACAGAGCTTG TCTGAGTGGC GCATGCAGAA CATTGCCCGA 
GACTCTGAGA ACAGCTCCGA GGAAGAATTC TTTGATGCCC ATGAAGGTTT CTCGGACAGT 
GATGAGGTCT TCCCCAAGGA GATGACCAAG TGGAATTCCA ATGATTTTAT CGATGCCTTT 
GCCTCCCCAA CCGAGGTGGA GGGGGTACCA GATCCTACAG TCATGGCTAC CAAAGGCATT 
GAAGATGGGG CCCGAGCTCC CAGGGACTCA GAGGGCCTAG ATGGAGCGGG GGATCTAGTG 
GTCGAGGCGT GCTCTGTGCA TGCCCTCTTC CTCATCCTGC ACAGCGGCAG CATCCTGGAC 
TCTGGCCCTG GAGACACCAA CTCCAAGCAG GCCGACGTGC AAACGCTGAG CACAGCCTTT 
GAGGCCGTCA CCCGAGTCCA TTTCCCCGAG GCCCTGGGTC ACGTGGCACT GCGGCTGGTG 
CCCTGTCCAC CCATTTGCGC GGCTGCCTAT GCTCTTGTCT CCAACCTGAG CCCCTACAGC 
CATGATGGGG ATAGCCTGTC CCGCTCCCAA GACCACATTC CGCTGGCTGC CCTGCCGCTC 
CTGGCCACCT CATCCTCTCG CTACCAGGGT GCCGTGGCCA CTGTCATCGC TCGCACCAAC 
CAGGCCTATG CAGCCTTCCT GCGCTCATCG GAGGGCACAG GTTTCTGCGG GCAGGTGGTG 
CTGATCGGAG ATGGTGTTGG TGGCATCCTG GGCTTTGATG CGCTCTGCCA CAGTGCCAGT 
GCAGGCCCAG GGAGTCGGGG CAGCAGCCGC CGTGGGAGCA TGAACAATGA GATGCTCTCC 
CCTGAAGTTG GTCCAGTACG GGACCCGCTG GCAGATGGGG TGGAGGTACT GGGTCGTGCT 
AGCCCAGAAC CCTCAGCCCT ACCTGCTCAG CGTACCTTCA GTGACATGGC CAATCCTGAT 
CCTGATGGCT CTCAGAACAG CCTCCAGGTA GCCTCCACAG CTACCTCCTC TGGGGAGCCC 
CGCCGTGCAA GCACAGCCTC CTGTCCACCT GCCAGTTCTG AGGCTCCTGA CGGCCCCACG 
AACGCTGCTC GCCTGGACTT CAAGGTCTCA GGCTTCTTCC TCTTTGGCTC CCCACTGGGC 
CTGGTGCTGG CTCTTCGCAA AACCGTGATG CCCGCCTTGG AGGTGGCTCA GCTGCGTCCA 
GCCTGCGAGC AGATCTACAA TCTCTTCCAT GCCGCTGACC CCTGTGCCTC CCGCCTGGAA 
CCCCTGCTGG CCCCCAAGTT CCAGGCCATC GCCCCCCTGG CTGTACCTCG TTACCAGAAG 
TTTCCCCTAG GAGACGGCTC GTCCTTGCTG CTGGCTGACA CTTTGCAAAC CCATTCGAGT 
CTCTTCCTGG AAGAGCTGGA GATGATGGTG CCCTCCACAC CCACCTCGGC CAGTGGTGCC 
TTCTGGAAGG GCAGTGAACT AGGCAATGAG CCAGCATCCC AGACAGCAGC CCCCAGCACC 
ACCAGCGAGG TTGTTAAGAG TAGATGGAAA GGAGGGAGGG AAGACACCCC CCGCCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531743.1
CDS	245..2764
Translation	MLIKEYHILLPMSLDEYQVAQLYMIQKKSREESSGEGSGVEILANRPYTDGPGGNGQYTHKVYHVGSHIPGWFRALLPKAALQVEEESWNAYPYTRTRYTCPFVEKFSIEIETYYLPDGGQQPNVFNLSGAERRQRIVDTIDIVRDAVAPGEYKAEEDPRLYRSAKTGRGPLADDWARTAAQTGPLMCAYKLCKVEFRYWGMQAKIEQFIHDVGLRRVMLRAHRQAWCWQDEWIELSMADIRALEEETARMLAQRMAKCNTGSEGPEAQTPGKSSTEARPGTSTAGTPDGPEAPPGPDASPDASFGKQWSSSSRSSYSSQHGGGVSPQSLSEWRMQNIARDSENSSEEEFFDAHEGFSDSDEVFPKEMTKWNSNDFIDAFASPTEVEGVPDPTVMATKGIEDGARAPRDSEGLDGAGDLVVEACSVHALFLILHSGSILDSGPGDTNSKQADVQTLSTAFEAVTRVHFPEALGHVALRLVPCPPICAAAYALVSNLSPYSHDGDSLSRSQDHIPLAALPLLATSSSRYQGAVATVIARTNQAYAAFLRSSEGTGFCGQVVLIGDGVGGILGFDALCHSASAGPGSRGSSRRGSMNNEMLSPEVGPVRDPLADGVEVLGRASPEPSALPAQRTFSDMANPDPDGSQNSLQVASTATSSGEPRRASTASCPPASSEAPDGPTNAARLDFKVSGFFLFGSPLGLVLALRKTVMPALEVAQLRPACEQIYNLFHAADPCASRLEPLLAPKFQAIAPLAVPRYQKFPLGDGSSLLLADTLQTHSSLFLEELEMMVPSTPTSASGAFWKGSELGNEPASQTAAPSTTSEVVKSRWKGGREDTPRP

Target ORF information:

RefSeq Version	XM_006531680.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphatidylinositol transfer protein, membrane-associated 1 (Pitpnm1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531680.3

ORF Insert Sequence:

ATGCTTATCA AGGAGTACCA CATCCTGCTG CCCATGAGCC TGGACGAGTA TCAAGTGGCC 
CAGCTCTACA TGATCCAGAA AAAGAGCCGT GAGGAGTCTA GTGGTGAGGG CAGCGGCGTG 
GAGATCCTGG CCAACCGGCC CTACACAGAT GGGCCTGGAG GCAACGGGCA GTATACACAC 
AAGGTGTACC ACGTGGGCTC CCACATCCCA GGCTGGTTCC GGGCACTGTT ACCCAAGGCT 
GCTCTGCAGG TAGAAGAGGA ATCTTGGAAC GCTTACCCAT ATACCCGGAC ACGGTACACC 
TGCCCCTTTG TGGAGAAGTT CTCCATTGAG ATAGAGACCT ACTACTTGCC TGATGGGGGG 
CAGCAACCTA ACGTCTTCAA CCTGAGTGGG GCTGAGAGAA GACAGAGAAT CGTGGATACC 
ATCGACATCG TGCGGGATGC AGTGGCCCCA GGAGAATACA AAGCGGAAGA GGACCCTCGG 
CTGTACCGCT CAGCCAAGAC AGGCCGAGGG CCGCTGGCTG ATGACTGGGC ACGGACAGCG 
GCCCAGACAG GACCTCTCAT GTGTGCCTAT AAGCTGTGCA AGGTTGAATT CCGCTACTGG 
GGCATGCAGG CCAAGATTGA GCAGTTCATC CATGACGTAG GTCTGCGCAG GGTGATGCTT 
CGCGCCCATC GCCAGGCCTG GTGTTGGCAA GATGAGTGGA TAGAACTGAG CATGGCTGAC 
ATCCGGGCAC TGGAGGAGGA GACTGCACGC ATGCTAGCGC AGCGTATGGC TAAGTGCAAC 
ACTGGCAGTG AGGGACCAGA GGCTCAGACC CCTGGGAAAT CCAGCACTGA GGCCCGACCT 
GGGACCAGCA CTGCTGGCAC CCCTGATGGA CCTGAGGCCC CTCCCGGCCC CGACGCCTCC 
CCAGATGCCA GCTTTGGGAA GCAGTGGTCC TCATCCTCCC GTTCCTCCTA CTCATCCCAA 
CATGGAGGCG GCGTGTCTCC ACAGAGCTTG TCTGAGTGGC GCATGCAGAA CATTGCCCGA 
GACTCTGAGA ACAGCTCCGA GGAAGAATTC TTTGATGCCC ATGAAGGTTT CTCGGACAGT 
GATGAGGTCT TCCCCAAGGA GATGACCAAG TGGAATTCCA ATGATTTTAT CGATGCCTTT 
GCCTCCCCAA CCGAGGTGGA GGGGGTACCA GATCCTACAG TCATGGCTAC CAAAGGCATT 
GAAGATGGGG CCCGAGCTCC CAGGGACTCA GAGGGCCTAG ATGGAGCGGG GGATCTAGTG 
GTCGAGGCGT GCTCTGTGCA TGCCCTCTTC CTCATCCTGC ACAGCGGCAG CATCCTGGAC 
TCTGGCCCTG GAGACACCAA CTCCAAGCAG GCCGACGTGC AAACGCTGAG CACAGCCTTT 
GAGGCCGTCA CCCGAGTCCA TTTCCCCGAG GCCCTGGGTC ACGTGGCACT GCGGCTGGTG 
CCCTGTCCAC CCATTTGCGC GGCTGCCTAT GCTCTTGTCT CCAACCTGAG CCCCTACAGC 
CATGATGGGG ATAGCCTGTC CCGCTCCCAA GACCACATTC CGCTGGCTGC CCTGCCGCTC 
CTGGCCACCT CATCCTCTCG CTACCAGGGT GCCGTGGCCA CTGTCATCGC TCGCACCAAC 
CAGGCCTATG CAGCCTTCCT GCGCTCATCG GAGGGCACAG GTTTCTGCGG GCAGGTGGTG 
CTGATCGGAG ATGGTGTTGG TGGCATCCTG GGCTTTGATG CGCTCTGCCA CAGTGCCAGT 
GCAGGCCCAG GGAGTCGGGG CAGCAGCCGC CGTGGGAGCA TGAACAATGA GATGCTCTCC 
CCTGAAGTTG GTCCAGTACG GGACCCGCTG GCAGATGGGG TGGAGGTACT GGGTCGTGCT 
AGCCCAGAAC CCTCAGCCCT ACCTGCTCAG CGTACCTTCA GTGACATGGC CAATCCTGAT 
CCTGATGGCT CTCAGAACAG CCTCCAGGTA GCCTCCACAG CTACCTCCTC TGGGGAGCCC 
CGCCGTGCAA GCACAGCCTC CTGTCCACCT GCCAGTTCTG AGGCTCCTGA CGGCCCCACG 
AACGCTGCTC GCCTGGACTT CAAGGTCTCA GGCTTCTTCC TCTTTGGCTC CCCACTGGGC 
CTGGTGCTGG CTCTTCGCAA AACCGTGATG CCCGCCTTGG AGGTGGCTCA GCTGCGTCCA 
GCCTGCGAGC AGATCTACAA TCTCTTCCAT GCCGCTGACC CCTGTGCCTC CCGCCTGGAA 
CCCCTGCTGG CCCCCAAGTT CCAGGCCATC GCCCCCCTGG CTGTACCTCG TTACCAGAAG 
TTTCCCCTAG GAGACGGCTC GTCCTTGCTG CTGGCTGACA CTTTGCAAAC CCATTCGAGT 
CTCTTCCTGG AAGAGCTGGA GATGATGGTG CCCTCCACAC CCACCTCGGC CAGTGGTGCC 
TTCTGGAAGG GCAGTGAACT AGGCAATGAG CCAGCATCCC AGACAGCAGC CCCCAGCACC 
ACCAGCGAGG TTGTTAAGAG TAGATGGAAA GGAGGGAGGG AAGACACCCC CCGCCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pitpnm1 ( XM_006531680.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531743.1 Mus musculus phosphatidylinositol transfer protein, membrane-associated 1 (Pitpnm1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu55028
Clone ID Related Accession (Same CDS sequence)	XM_006531680.3 , XM_006531680.4
Accession Version	XM_006531680.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	membrane-associated phosphatidylinositol transfer protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006531680.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTATCA AGGAGTACCA CATCCTGCTG CCCATGAGCC TGGACGAGTA TCAAGTGGCC 
CAGCTCTACA TGATCCAGAA AAAGAGCCGT GAGGAGTCTA GTGGTGAGGG CAGCGGCGTG 
GAGATCCTGG CCAACCGGCC CTACACAGAT GGGCCTGGAG GCAACGGGCA GTATACACAC 
AAGGTGTACC ACGTGGGCTC CCACATCCCA GGCTGGTTCC GGGCACTGTT ACCCAAGGCT 
GCTCTGCAGG TAGAAGAGGA ATCTTGGAAC GCTTACCCAT ATACCCGGAC ACGGTACACC 
TGCCCCTTTG TGGAGAAGTT CTCCATTGAG ATAGAGACCT ACTACTTGCC TGATGGGGGG 
CAGCAACCTA ACGTCTTCAA CCTGAGTGGG GCTGAGAGAA GACAGAGAAT CGTGGATACC 
ATCGACATCG TGCGGGATGC AGTGGCCCCA GGAGAATACA AAGCGGAAGA GGACCCTCGG 
CTGTACCGCT CAGCCAAGAC AGGCCGAGGG CCGCTGGCTG ATGACTGGGC ACGGACAGCG 
GCCCAGACAG GACCTCTCAT GTGTGCCTAT AAGCTGTGCA AGGTTGAATT CCGCTACTGG 
GGCATGCAGG CCAAGATTGA GCAGTTCATC CATGACGTAG GTCTGCGCAG GGTGATGCTT 
CGCGCCCATC GCCAGGCCTG GTGTTGGCAA GATGAGTGGA TAGAACTGAG CATGGCTGAC 
ATCCGGGCAC TGGAGGAGGA GACTGCACGC ATGCTAGCGC AGCGTATGGC TAAGTGCAAC 
ACTGGCAGTG AGGGACCAGA GGCTCAGACC CCTGGGAAAT CCAGCACTGA GGCCCGACCT 
GGGACCAGCA CTGCTGGCAC CCCTGATGGA CCTGAGGCCC CTCCCGGCCC CGACGCCTCC 
CCAGATGCCA GCTTTGGGAA GCAGTGGTCC TCATCCTCCC GTTCCTCCTA CTCATCCCAA 
CATGGAGGCG GCGTGTCTCC ACAGAGCTTG TCTGAGTGGC GCATGCAGAA CATTGCCCGA 
GACTCTGAGA ACAGCTCCGA GGAAGAATTC TTTGATGCCC ATGAAGGTTT CTCGGACAGT 
GATGAGGTCT TCCCCAAGGA GATGACCAAG TGGAATTCCA ATGATTTTAT CGATGCCTTT 
GCCTCCCCAA CCGAGGTGGA GGGGGTACCA GATCCTACAG TCATGGCTAC CAAAGGCATT 
GAAGATGGGG CCCGAGCTCC CAGGGACTCA GAGGGCCTAG ATGGAGCGGG GGATCTAGTG 
GTCGAGGCGT GCTCTGTGCA TGCCCTCTTC CTCATCCTGC ACAGCGGCAG CATCCTGGAC 
TCTGGCCCTG GAGACACCAA CTCCAAGCAG GCCGACGTGC AAACGCTGAG CACAGCCTTT 
GAGGCCGTCA CCCGAGTCCA TTTCCCCGAG GCCCTGGGTC ACGTGGCACT GCGGCTGGTG 
CCCTGTCCAC CCATTTGCGC GGCTGCCTAT GCTCTTGTCT CCAACCTGAG CCCCTACAGC 
CATGATGGGG ATAGCCTGTC CCGCTCCCAA GACCACATTC CGCTGGCTGC CCTGCCGCTC 
CTGGCCACCT CATCCTCTCG CTACCAGGGT GCCGTGGCCA CTGTCATCGC TCGCACCAAC 
CAGGCCTATG CAGCCTTCCT GCGCTCATCG GAGGGCACAG GTTTCTGCGG GCAGGTGGTG 
CTGATCGGAG ATGGTGTTGG TGGCATCCTG GGCTTTGATG CGCTCTGCCA CAGTGCCAGT 
GCAGGCCCAG GGAGTCGGGG CAGCAGCCGC CGTGGGAGCA TGAACAATGA GATGCTCTCC 
CCTGAAGTTG GTCCAGTACG GGACCCGCTG GCAGATGGGG TGGAGGTACT GGGTCGTGCT 
AGCCCAGAAC CCTCAGCCCT ACCTGCTCAG CGTACCTTCA GTGACATGGC CAATCCTGAT 
CCTGATGGCT CTCAGAACAG CCTCCAGGTA GCCTCCACAG CTACCTCCTC TGGGGAGCCC 
CGCCGTGCAA GCACAGCCTC CTGTCCACCT GCCAGTTCTG AGGCTCCTGA CGGCCCCACG 
AACGCTGCTC GCCTGGACTT CAAGGTCTCA GGCTTCTTCC TCTTTGGCTC CCCACTGGGC 
CTGGTGCTGG CTCTTCGCAA AACCGTGATG CCCGCCTTGG AGGTGGCTCA GCTGCGTCCA 
GCCTGCGAGC AGATCTACAA TCTCTTCCAT GCCGCTGACC CCTGTGCCTC CCGCCTGGAA 
CCCCTGCTGG CCCCCAAGTT CCAGGCCATC GCCCCCCTGG CTGTACCTCG TTACCAGAAG 
TTTCCCCTAG GAGACGGCTC GTCCTTGCTG CTGGCTGACA CTTTGCAAAC CCATTCGAGT 
CTCTTCCTGG AAGAGCTGGA GATGATGGTG CCCTCCACAC CCACCTCGGC CAGTGGTGCC 
TTCTGGAAGG GCAGTGAACT AGGCAATGAG CCAGCATCCC AGACAGCAGC CCCCAGCACC 
ACCAGCGAGG TTGTTAAGAG TAGATGGAAA GGAGGGAGGG AAGACACCCC CCGCCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531743.1
CDS	377..2896
Translation	MLIKEYHILLPMSLDEYQVAQLYMIQKKSREESSGEGSGVEILANRPYTDGPGGNGQYTHKVYHVGSHIPGWFRALLPKAALQVEEESWNAYPYTRTRYTCPFVEKFSIEIETYYLPDGGQQPNVFNLSGAERRQRIVDTIDIVRDAVAPGEYKAEEDPRLYRSAKTGRGPLADDWARTAAQTGPLMCAYKLCKVEFRYWGMQAKIEQFIHDVGLRRVMLRAHRQAWCWQDEWIELSMADIRALEEETARMLAQRMAKCNTGSEGPEAQTPGKSSTEARPGTSTAGTPDGPEAPPGPDASPDASFGKQWSSSSRSSYSSQHGGGVSPQSLSEWRMQNIARDSENSSEEEFFDAHEGFSDSDEVFPKEMTKWNSNDFIDAFASPTEVEGVPDPTVMATKGIEDGARAPRDSEGLDGAGDLVVEACSVHALFLILHSGSILDSGPGDTNSKQADVQTLSTAFEAVTRVHFPEALGHVALRLVPCPPICAAAYALVSNLSPYSHDGDSLSRSQDHIPLAALPLLATSSSRYQGAVATVIARTNQAYAAFLRSSEGTGFCGQVVLIGDGVGGILGFDALCHSASAGPGSRGSSRRGSMNNEMLSPEVGPVRDPLADGVEVLGRASPEPSALPAQRTFSDMANPDPDGSQNSLQVASTATSSGEPRRASTASCPPASSEAPDGPTNAARLDFKVSGFFLFGSPLGLVLALRKTVMPALEVAQLRPACEQIYNLFHAADPCASRLEPLLAPKFQAIAPLAVPRYQKFPLGDGSSLLLADTLQTHSSLFLEELEMMVPSTPTSASGAFWKGSELGNEPASQTAAPSTTSEVVKSRWKGGREDTPRP

Target ORF information:

RefSeq Version	XM_006531680.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphatidylinositol transfer protein, membrane-associated 1 (Pitpnm1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531680.4

ORF Insert Sequence:

ATGCTTATCA AGGAGTACCA CATCCTGCTG CCCATGAGCC TGGACGAGTA TCAAGTGGCC 
CAGCTCTACA TGATCCAGAA AAAGAGCCGT GAGGAGTCTA GTGGTGAGGG CAGCGGCGTG 
GAGATCCTGG CCAACCGGCC CTACACAGAT GGGCCTGGAG GCAACGGGCA GTATACACAC 
AAGGTGTACC ACGTGGGCTC CCACATCCCA GGCTGGTTCC GGGCACTGTT ACCCAAGGCT 
GCTCTGCAGG TAGAAGAGGA ATCTTGGAAC GCTTACCCAT ATACCCGGAC ACGGTACACC 
TGCCCCTTTG TGGAGAAGTT CTCCATTGAG ATAGAGACCT ACTACTTGCC TGATGGGGGG 
CAGCAACCTA ACGTCTTCAA CCTGAGTGGG GCTGAGAGAA GACAGAGAAT CGTGGATACC 
ATCGACATCG TGCGGGATGC AGTGGCCCCA GGAGAATACA AAGCGGAAGA GGACCCTCGG 
CTGTACCGCT CAGCCAAGAC AGGCCGAGGG CCGCTGGCTG ATGACTGGGC ACGGACAGCG 
GCCCAGACAG GACCTCTCAT GTGTGCCTAT AAGCTGTGCA AGGTTGAATT CCGCTACTGG 
GGCATGCAGG CCAAGATTGA GCAGTTCATC CATGACGTAG GTCTGCGCAG GGTGATGCTT 
CGCGCCCATC GCCAGGCCTG GTGTTGGCAA GATGAGTGGA TAGAACTGAG CATGGCTGAC 
ATCCGGGCAC TGGAGGAGGA GACTGCACGC ATGCTAGCGC AGCGTATGGC TAAGTGCAAC 
ACTGGCAGTG AGGGACCAGA GGCTCAGACC CCTGGGAAAT CCAGCACTGA GGCCCGACCT 
GGGACCAGCA CTGCTGGCAC CCCTGATGGA CCTGAGGCCC CTCCCGGCCC CGACGCCTCC 
CCAGATGCCA GCTTTGGGAA GCAGTGGTCC TCATCCTCCC GTTCCTCCTA CTCATCCCAA 
CATGGAGGCG GCGTGTCTCC ACAGAGCTTG TCTGAGTGGC GCATGCAGAA CATTGCCCGA 
GACTCTGAGA ACAGCTCCGA GGAAGAATTC TTTGATGCCC ATGAAGGTTT CTCGGACAGT 
GATGAGGTCT TCCCCAAGGA GATGACCAAG TGGAATTCCA ATGATTTTAT CGATGCCTTT 
GCCTCCCCAA CCGAGGTGGA GGGGGTACCA GATCCTACAG TCATGGCTAC CAAAGGCATT 
GAAGATGGGG CCCGAGCTCC CAGGGACTCA GAGGGCCTAG ATGGAGCGGG GGATCTAGTG 
GTCGAGGCGT GCTCTGTGCA TGCCCTCTTC CTCATCCTGC ACAGCGGCAG CATCCTGGAC 
TCTGGCCCTG GAGACACCAA CTCCAAGCAG GCCGACGTGC AAACGCTGAG CACAGCCTTT 
GAGGCCGTCA CCCGAGTCCA TTTCCCCGAG GCCCTGGGTC ACGTGGCACT GCGGCTGGTG 
CCCTGTCCAC CCATTTGCGC GGCTGCCTAT GCTCTTGTCT CCAACCTGAG CCCCTACAGC 
CATGATGGGG ATAGCCTGTC CCGCTCCCAA GACCACATTC CGCTGGCTGC CCTGCCGCTC 
CTGGCCACCT CATCCTCTCG CTACCAGGGT GCCGTGGCCA CTGTCATCGC TCGCACCAAC 
CAGGCCTATG CAGCCTTCCT GCGCTCATCG GAGGGCACAG GTTTCTGCGG GCAGGTGGTG 
CTGATCGGAG ATGGTGTTGG TGGCATCCTG GGCTTTGATG CGCTCTGCCA CAGTGCCAGT 
GCAGGCCCAG GGAGTCGGGG CAGCAGCCGC CGTGGGAGCA TGAACAATGA GATGCTCTCC 
CCTGAAGTTG GTCCAGTACG GGACCCGCTG GCAGATGGGG TGGAGGTACT GGGTCGTGCT 
AGCCCAGAAC CCTCAGCCCT ACCTGCTCAG CGTACCTTCA GTGACATGGC CAATCCTGAT 
CCTGATGGCT CTCAGAACAG CCTCCAGGTA GCCTCCACAG CTACCTCCTC TGGGGAGCCC 
CGCCGTGCAA GCACAGCCTC CTGTCCACCT GCCAGTTCTG AGGCTCCTGA CGGCCCCACG 
AACGCTGCTC GCCTGGACTT CAAGGTCTCA GGCTTCTTCC TCTTTGGCTC CCCACTGGGC 
CTGGTGCTGG CTCTTCGCAA AACCGTGATG CCCGCCTTGG AGGTGGCTCA GCTGCGTCCA 
GCCTGCGAGC AGATCTACAA TCTCTTCCAT GCCGCTGACC CCTGTGCCTC CCGCCTGGAA 
CCCCTGCTGG CCCCCAAGTT CCAGGCCATC GCCCCCCTGG CTGTACCTCG TTACCAGAAG 
TTTCCCCTAG GAGACGGCTC GTCCTTGCTG CTGGCTGACA CTTTGCAAAC CCATTCGAGT 
CTCTTCCTGG AAGAGCTGGA GATGATGGTG CCCTCCACAC CCACCTCGGC CAGTGGTGCC 
TTCTGGAAGG GCAGTGAACT AGGCAATGAG CCAGCATCCC AGACAGCAGC CCCCAGCACC 
ACCAGCGAGG TTGTTAAGAG TAGATGGAAA GGAGGGAGGG AAGACACCCC CCGCCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pitpnm1 ( XM_030250773.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106633.1 Mus musculus phosphatidylinositol transfer protein, membrane-associated 1 (Pitpnm1), transcript variant X6, mRNA.

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CloneID	OMu87495
Clone ID Related Accession (Same CDS sequence)	XM_030250773.1
Accession Version	XM_030250773.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	membrane-associated phosphatidylinositol transfer protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTCCC CTGAAGTTGG TCCAGTACGG GACCCGCTGG CAGATGGGGT GGAGGTACTG 
GGTCGTGCTA GCCCAGAACC CTCAGCCCTA CCTGCTCAGC GTACCTTCAG TGACATGGCC 
AATCCTGATC CTGATGGCTC TCAGAACAGC CTCCAGGTAG CCTCCACAGC TACCTCCTCT 
GGGGAGCCCC GCCGTGCAAG CACAGCCTCC TGTCCACCTG CCAGTTCTGA GGCTCCTGAC 
GGCCCCACGA ACGCTGCTCG CCTGGACTTC AAGGTCTCAG GCTTCTTCCT CTTTGGCTCC 
CCACTGGGCC TGGTGCTGGC TCTTCGCAAA ACCGTGATGC CCGCCTTGGA GGTGGCTCAG 
CTGCGTCCAG CCTGCGAGCA GATCTACAAT CTCTTCCATG CCGCTGACCC CTGTGCCTCC 
CGCCTGGAAC CCCTGCTGGC CCCCAAGTTC CAGGCCATCG CCCCCCTGGC TGTACCTCGT 
TACCAGAAGT TTCCCCTAGG AGACGGCTCG TCCTTGCTGC TGGCTGACAC TTTGCAAACC 
CATTCGAGTC TCTTCCTGGA AGAGCTGGAG ATGATGGTGC CCTCCACACC CACCTCGGCC 
AGTGGTGCCT TCTGGAAGGG CAGTGAACTA GGCAATGAGC CAGCATCCCA GACAGCAGCC 
CCCAGCACCA CCAGCGAGGT TGTTAAGATC CTGGACCGCT GGTGGGGAAA CAAGCGGATC 
GACTATTCAC TGTACTGCCC CGAGGCACTC ACGGCCTTCC CCACGGTCAC GCTGCCCCAC 
CTCTTCCACG CCAGCTACTG GGAATCAGCG GATGTAGTGG CCTTCATTCT GCGCCAGGTC 
ATTGAGAAGG AGCGGCCACA GCTGACAGAG TGTGAGGAGC CATCCATCTA TAGCCCCGCC 
TTCCCCAGGG AGAAGTGGCA GCGCAAACGC ACACAGGTCA AGATCCGGAA CGTCACTTCC 
AACCACCGAG CGAGTGACAC CGTGGTATGT GAGGGCCGTC CCCAGGTGCT GAATGGGCGC 
TTCATGTATG GACCATTGGA TGTGGTCACG CTCACTGGAG AGAAGGTGGA TGTCTACGTC 
ATGACACAGC CACTGTCAGG CAAGTGGATC CACTTTGGCA CAGAGGTCAC TAACAGCTCA 
GGCCGTCTCA CCTTCCCAGT GCCCTCAGAA CGTGCACTGG GCATTGGTGT CTACCCTGTG 
CGCATGGTGG TCAGGGGAGA CCACACCTAT GCCGAGTGCT GTCTGACTGT GGTGTCCCGA 
GGCACAGAAG CTGTGGTCTT CAGCATCGAT GGTTCTTTCA CTGCCAGTGT CTCTATCATG 
GGCAGCGACC CCAAGGTGCG CGCTGGCGCA GTGGACGTGG TCAGGCACTG GCAGGACTCT 
GGCTACTTGA TTGTGTATGT AACTGGCCGG CCTGACATGC AAAAGCACCG TGTAGTGGCC 
TGGCTGTCGC AACACAACTT CCCCCATGGT GTTGTCTCCT TCTGTGATGG CCTCACCCAC 
GACCCGCTGC GGCAGAAAGC CATGTTTCTG CAGAGCCTGG TGCAAGAGGT AGAACTGAAC 
ATCGTGGCTG GATATGGGTC ACCAAAAGAT GTGGCAGTGT ATGCAGCACT GGGGCTCTCT 
CCGAGCCAGA CCTACATTGT TGGCCGTGCT GTGCGCAAAC TGCAGGCACA GTGTCAGTTC 
CTGTCAGATG GCTATGTGGC CCACTTGGGC CAGCTGGAGG CAGGCTCCCA CTCCCATGCT 
CCCTCAGGAC CTCCGAGAGC CGCTCTGGCC AAGAGTAGCT ATGCTGTGGC TGCCCCTGTG 
GACTTCCTCC GGAAGCAGAG CCAGCTGCTT CGATCCAGAG GCCCTAGCCA GGTGGACCGT 
GAGGGTCCAG GAACACCTCC CACCACCCTG GCCAGGGGCA AGACTCGCAG CATCAGCCTC 
AAGTTGGACA GTGAAGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106633.1
CDS	2873..4813
Translation	MLSPEVGPVRDPLADGVEVLGRASPEPSALPAQRTFSDMANPDPDGSQNSLQVASTATSSGEPRRASTASCPPASSEAPDGPTNAARLDFKVSGFFLFGSPLGLVLALRKTVMPALEVAQLRPACEQIYNLFHAADPCASRLEPLLAPKFQAIAPLAVPRYQKFPLGDGSSLLLADTLQTHSSLFLEELEMMVPSTPTSASGAFWKGSELGNEPASQTAAPSTTSEVVKILDRWWGNKRIDYSLYCPEALTAFPTVTLPHLFHASYWESADVVAFILRQVIEKERPQLTECEEPSIYSPAFPREKWQRKRTQVKIRNVTSNHRASDTVVCEGRPQVLNGRFMYGPLDVVTLTGEKVDVYVMTQPLSGKWIHFGTEVTNSSGRLTFPVPSERALGIGVYPVRMVVRGDHTYAECCLTVVSRGTEAVVFSIDGSFTASVSIMGSDPKVRAGAVDVVRHWQDSGYLIVYVTGRPDMQKHRVVAWLSQHNFPHGVVSFCDGLTHDPLRQKAMFLQSLVQEVELNIVAGYGSPKDVAVYAALGLSPSQTYIVGRAVRKLQAQCQFLSDGYVAHLGQLEAGSHSHAPSGPPRAALAKSSYAVAAPVDFLRKQSQLLRSRGPSQVDREGPGTPPTTLARGKTRSISLKLDSEE

Target ORF information:

RefSeq Version	XM_030250773.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphatidylinositol transfer protein, membrane-associated 1 (Pitpnm1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250773.1

ORF Insert Sequence:

ATGCTCTCCC CTGAAGTTGG TCCAGTACGG GACCCGCTGG CAGATGGGGT GGAGGTACTG 
GGTCGTGCTA GCCCAGAACC CTCAGCCCTA CCTGCTCAGC GTACCTTCAG TGACATGGCC 
AATCCTGATC CTGATGGCTC TCAGAACAGC CTCCAGGTAG CCTCCACAGC TACCTCCTCT 
GGGGAGCCCC GCCGTGCAAG CACAGCCTCC TGTCCACCTG CCAGTTCTGA GGCTCCTGAC 
GGCCCCACGA ACGCTGCTCG CCTGGACTTC AAGGTCTCAG GCTTCTTCCT CTTTGGCTCC 
CCACTGGGCC TGGTGCTGGC TCTTCGCAAA ACCGTGATGC CCGCCTTGGA GGTGGCTCAG 
CTGCGTCCAG CCTGCGAGCA GATCTACAAT CTCTTCCATG CCGCTGACCC CTGTGCCTCC 
CGCCTGGAAC CCCTGCTGGC CCCCAAGTTC CAGGCCATCG CCCCCCTGGC TGTACCTCGT 
TACCAGAAGT TTCCCCTAGG AGACGGCTCG TCCTTGCTGC TGGCTGACAC TTTGCAAACC 
CATTCGAGTC TCTTCCTGGA AGAGCTGGAG ATGATGGTGC CCTCCACACC CACCTCGGCC 
AGTGGTGCCT TCTGGAAGGG CAGTGAACTA GGCAATGAGC CAGCATCCCA GACAGCAGCC 
CCCAGCACCA CCAGCGAGGT TGTTAAGATC CTGGACCGCT GGTGGGGAAA CAAGCGGATC 
GACTATTCAC TGTACTGCCC CGAGGCACTC ACGGCCTTCC CCACGGTCAC GCTGCCCCAC 
CTCTTCCACG CCAGCTACTG GGAATCAGCG GATGTAGTGG CCTTCATTCT GCGCCAGGTC 
ATTGAGAAGG AGCGGCCACA GCTGACAGAG TGTGAGGAGC CATCCATCTA TAGCCCCGCC 
TTCCCCAGGG AGAAGTGGCA GCGCAAACGC ACACAGGTCA AGATCCGGAA CGTCACTTCC 
AACCACCGAG CGAGTGACAC CGTGGTATGT GAGGGCCGTC CCCAGGTGCT GAATGGGCGC 
TTCATGTATG GACCATTGGA TGTGGTCACG CTCACTGGAG AGAAGGTGGA TGTCTACGTC 
ATGACACAGC CACTGTCAGG CAAGTGGATC CACTTTGGCA CAGAGGTCAC TAACAGCTCA 
GGCCGTCTCA CCTTCCCAGT GCCCTCAGAA CGTGCACTGG GCATTGGTGT CTACCCTGTG 
CGCATGGTGG TCAGGGGAGA CCACACCTAT GCCGAGTGCT GTCTGACTGT GGTGTCCCGA 
GGCACAGAAG CTGTGGTCTT CAGCATCGAT GGTTCTTTCA CTGCCAGTGT CTCTATCATG 
GGCAGCGACC CCAAGGTGCG CGCTGGCGCA GTGGACGTGG TCAGGCACTG GCAGGACTCT 
GGCTACTTGA TTGTGTATGT AACTGGCCGG CCTGACATGC AAAAGCACCG TGTAGTGGCC 
TGGCTGTCGC AACACAACTT CCCCCATGGT GTTGTCTCCT TCTGTGATGG CCTCACCCAC 
GACCCGCTGC GGCAGAAAGC CATGTTTCTG CAGAGCCTGG TGCAAGAGGT AGAACTGAAC 
ATCGTGGCTG GATATGGGTC ACCAAAAGAT GTGGCAGTGT ATGCAGCACT GGGGCTCTCT 
CCGAGCCAGA CCTACATTGT TGGCCGTGCT GTGCGCAAAC TGCAGGCACA GTGTCAGTTC 
CTGTCAGATG GCTATGTGGC CCACTTGGGC CAGCTGGAGG CAGGCTCCCA CTCCCATGCT 
CCCTCAGGAC CTCCGAGAGC CGCTCTGGCC AAGAGTAGCT ATGCTGTGGC TGCCCCTGTG 
GACTTCCTCC GGAAGCAGAG CCAGCTGCTT CGATCCAGAG GCCCTAGCCA GGTGGACCGT 
GAGGGTCCAG GAACACCTCC CACCACCCTG GCCAGGGGCA AGACTCGCAG CATCAGCCTC 
AAGTTGGACA GTGAAGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pitpnm1 ( NM_008851.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032877.1 Mus musculus phosphatidylinositol transfer protein, membrane-associated 1 (Pitpnm1), transcript variant 1, mRNA.

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CloneID	OMu18332
Clone ID Related Accession (Same CDS sequence)	NM_008851.4
Accession Version	NM_008851.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	membrane-associated phosphatidylinositol transfer protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC109138.10, CJ097166.1, BY261372.1 and BC048150.1. On Jan 31, 2013 this sequence version replaced NM_008851.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the supported protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y08922.1, SRR1660815.123960.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTTATCA AGGAGTACCA CATCCTGCTG CCCATGAGCC TGGACGAGTA TCAAGTGGCC 
CAGCTCTACA TGATCCAGAA AAAGAGCCGT GAGGAGTCTA GTGGTGAGGG CAGCGGCGTG 
GAGATCCTGG CCAACCGGCC CTACACAGAT GGGCCTGGAG GCAACGGGCA GTATACACAC 
AAGGTGTACC ACGTGGGCTC CCACATCCCA GGCTGGTTCC GGGCACTGTT ACCCAAGGCT 
GCTCTGCAGG TAGAAGAGGA ATCTTGGAAC GCTTACCCAT ATACCCGGAC ACGGTACACC 
TGCCCCTTTG TGGAGAAGTT CTCCATTGAG ATAGAGACCT ACTACTTGCC TGATGGGGGG 
CAGCAACCTA ACGTCTTCAA CCTGAGTGGG GCTGAGAGAA GACAGAGAAT CGTGGATACC 
ATCGACATCG TGCGGGATGC AGTGGCCCCA GGAGAATACA AAGCGGAAGA GGACCCTCGG 
CTGTACCGCT CAGCCAAGAC AGGCCGAGGG CCGCTGGCTG ATGACTGGGC ACGGACAGCG 
GCCCAGACAG GACCTCTCAT GTGTGCCTAT AAGCTGTGCA AGGTTGAATT CCGCTACTGG 
GGCATGCAGG CCAAGATTGA GCAGTTCATC CATGACGTAG GTCTGCGCAG GGTGATGCTT 
CGCGCCCATC GCCAGGCCTG GTGTTGGCAA GATGAGTGGA TAGAACTGAG CATGGCTGAC 
ATCCGGGCAC TGGAGGAGGA GACTGCACGC ATGCTAGCGC AGCGTATGGC TAAGTGCAAC 
ACTGGCAGTG AGGGACCAGA GGCTCAGACC CCTGGGAAAT CCAGCACTGA GGCCCGACCT 
GGGACCAGCA CTGCTGGCAC CCCTGATGGA CCTGAGGCCC CTCCCGGCCC CGACGCCTCC 
CCAGATGCCA GCTTTGGGAA GCAGTGGTCC TCATCCTCCC GTTCCTCCTA CTCATCCCAA 
CATGGAGGCG GCGTGTCTCC ACAGAGCTTG TCTGAGTGGC GCATGCAGAA CATTGCCCGA 
GACTCTGAGA ACAGCTCCGA GGAAGAATTC TTTGATGCCC ATGAAGGTTT CTCGGACAGT 
GATGAGGTCT TCCCCAAGGA GATGACCAAG TGGAATTCCA ATGATTTTAT CGATGCCTTT 
GCCTCCCCAA CCGAGGTGGA GGGGGTACCA GATCCTACAG TCATGGCTAC CAAAGGCATT 
GAAGATGGGG CCCGAGCTCC CAGGGACTCA GAGGGCCTAG ATGGAGCGGG GGATCTAGTG 
GTCGAGGCGT GCTCTGTGCA TGCCCTCTTC CTCATCCTGC ACAGCGGCAG CATCCTGGAC 
TCTGGCCCTG GAGACACCAA CTCCAAGCAG GCCGACGTGC AAACGCTGAG CACAGCCTTT 
GAGGCCGTCA CCCGAGTCCA TTTCCCCGAG GCCCTGGGTC ACGTGGCACT GCGGCTGGTG 
CCCTGTCCAC CCATTTGCGC GGCTGCCTAT GCTCTTGTCT CCAACCTGAG CCCCTACAGC 
CATGATGGGG ATAGCCTGTC CCGCTCCCAA GACCACATTC CGCTGGCTGC CCTGCCGCTC 
CTGGCCACCT CATCCTCTCG CTACCAGGGT GCCGTGGCCA CTGTCATCGC TCGCACCAAC 
CAGGCCTATG CAGCCTTCCT GCGCTCATCG GAGGGCACAG GTTTCTGCGG GCAGGTGGTG 
CTGATCGGAG ATGGTGTTGG TGGCATCCTG GGCTTTGATG CGCTCTGCCA CAGTGCCAGT 
GCAGGCCCAG GGAGTCGGGG CAGCAGCCGC CGTGGGAGCA TGAACAATGA GATGCTCTCC 
CCTGAAGTTG GTCCAGTACG GGACCCGCTG GCAGATGGGG TGGAGGTACT GGGTCGTGCT 
AGCCCAGAAC CCTCAGCCCT ACCTGCTCAG CGTACCTTCA GTGACATGGC CAATCCTGAT 
CCTGATGGCT CTCAGAACAG CCTCCAGGTA GCCTCCACAG CTACCTCCTC TGGGGAGCCC 
CGCCGTGCAA GCACAGCCTC CTGTCCACCT GCCAGTTCTG AGGCTCCTGA CGGCCCCACG 
AACGCTGCTC GCCTGGACTT CAAGGTCTCA GGCTTCTTCC TCTTTGGCTC CCCACTGGGC 
CTGGTGCTGG CTCTTCGCAA AACCGTGATG CCCGCCTTGG AGGTGGCTCA GCTGCGTCCA 
GCCTGCGAGC AGATCTACAA TCTCTTCCAT GCCGCTGACC CCTGTGCCTC CCGCCTGGAA 
CCCCTGCTGG CCCCCAAGTT CCAGGCCATC GCCCCCCTGG CTGTACCTCG TTACCAGAAG 
TTTCCCCTAG GAGACGGCTC GTCCTTGCTG CTGGCTGACA CTTTGCAAAC CCATTCGAGT 
CTCTTCCTGG AAGAGCTGGA GATGATGGTG CCCTCCACAC CCACCTCGGC CAGTGGTGCC 
TTCTGGAAGG GCAGTGAACT AGGCAATGAG CCAGCATCCC AGACAGCAGC CCCCAGCACC 
ACCAGCGAGG TTGTTAAGAT CCTGGACCGC TGGTGGGGAA ACAAGCGGAT CGACTATTCA 
CTGTACTGCC CCGAGGCACT CACGGCCTTC CCCACGGTCA CGCTGCCCCA CCTCTTCCAC 
GCCAGCTACT GGGAATCAGC GGATGTAGTG GCCTTCATTC TGCGCCAGGT CATTGAGAAG 
GAGCGGCCAC AGCTGACAGA GTGTGAGGAG CCATCCATCT ATAGCCCCGC CTTCCCCAGG 
GAGAAGTGGC AGCGCAAACG CACACAGGTC AAGATCCGGA ACGTCACTTC CAACCACCGA 
GCGAGTGACA CCGTGGTATG TGAGGGCCGT CCCCAGGTGC TGAATGGGCG CTTCATGTAT 
GGACCATTGG ATGTGGTCAC GCTCACTGGA GAGAAGGTGG ATGTCTACGT CATGACACAG 
CCACTGTCAG GCAAGTGGAT CCACTTTGGC ACAGAGGTCA CTAACAGCTC AGGCCGTCTC 
ACCTTCCCAG TGCCCTCAGA ACGTGCACTG GGCATTGGTG TCTACCCTGT GCGCATGGTG 
GTCAGGGGAG ACCACACCTA TGCCGAGTGC TGTCTGACTG TGGTGTCCCG AGGCACAGAA 
GCTGTGGTCT TCAGCATCGA TGGTTCTTTC ACTGCCAGTG TCTCTATCAT GGGCAGCGAC 
CCCAAGGTGC GCGCTGGCGC AGTGGACGTG GTCAGGCACT GGCAGGACTC TGGCTACTTG 
ATTGTGTATG TAACTGGCCG GCCTGACATG CAAAAGCACC GTGTAGTGGC CTGGCTGTCG 
CAACACAACT TCCCCCATGG TGTTGTCTCC TTCTGTGATG GCCTCACCCA CGACCCGCTG 
CGGCAGAAAG CCATGTTTCT GCAGAGCCTG GTGCAAGAGG TAGAACTGAA CATCGTGGCT 
GGATATGGGT CACCAAAAGA TGTGGCAGTG TATGCAGCAC TGGGGCTCTC TCCGAGCCAG 
ACCTACATTG TTGGCCGTGC TGTGCGCAAA CTGCAGGCAC AGTGTCAGTT CCTGTCAGAT 
GGCTATGTGG CCCACTTGGG CCAGCTGGAG GCAGGCTCCC ACTCCCATGC TCCCTCAGGA 
CCTCCGAGAG CCGCTCTGGC CAAGAGTAGC TATGCTGTGG CTGCCCCTGT GGACTTCCTC 
CGGAAGCAGA GCCAGCTGCT TCGATCCAGA GGCCCTAGCC AGGTGGACCG TGAGGGTCCA 
GGAACACCTC CCACCACCCT GGCCAGGGGC AAGACTCGCA GCATCAGCCT CAAGTTGGAC 
AGTGAAGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032877.1
CDS	564..4295
Translation	MLIKEYHILLPMSLDEYQVAQLYMIQKKSREESSGEGSGVEILANRPYTDGPGGNGQYTHKVYHVGSHIPGWFRALLPKAALQVEEESWNAYPYTRTRYTCPFVEKFSIEIETYYLPDGGQQPNVFNLSGAERRQRIVDTIDIVRDAVAPGEYKAEEDPRLYRSAKTGRGPLADDWARTAAQTGPLMCAYKLCKVEFRYWGMQAKIEQFIHDVGLRRVMLRAHRQAWCWQDEWIELSMADIRALEEETARMLAQRMAKCNTGSEGPEAQTPGKSSTEARPGTSTAGTPDGPEAPPGPDASPDASFGKQWSSSSRSSYSSQHGGGVSPQSLSEWRMQNIARDSENSSEEEFFDAHEGFSDSDEVFPKEMTKWNSNDFIDAFASPTEVEGVPDPTVMATKGIEDGARAPRDSEGLDGAGDLVVEACSVHALFLILHSGSILDSGPGDTNSKQADVQTLSTAFEAVTRVHFPEALGHVALRLVPCPPICAAAYALVSNLSPYSHDGDSLSRSQDHIPLAALPLLATSSSRYQGAVATVIARTNQAYAAFLRSSEGTGFCGQVVLIGDGVGGILGFDALCHSASAGPGSRGSSRRGSMNNEMLSPEVGPVRDPLADGVEVLGRASPEPSALPAQRTFSDMANPDPDGSQNSLQVASTATSSGEPRRASTASCPPASSEAPDGPTNAARLDFKVSGFFLFGSPLGLVLALRKTVMPALEVAQLRPACEQIYNLFHAADPCASRLEPLLAPKFQAIAPLAVPRYQKFPLGDGSSLLLADTLQTHSSLFLEELEMMVPSTPTSASGAFWKGSELGNEPASQTAAPSTTSEVVKILDRWWGNKRIDYSLYCPEALTAFPTVTLPHLFHASYWESADVVAFILRQVIEKERPQLTECEEPSIYSPAFPREKWQRKRTQVKIRNVTSNHRASDTVVCEGRPQVLNGRFMYGPLDVVTLTGEKVDVYVMTQPLSGKWIHFGTEVTNSSGRLTFPVPSERALGIGVYPVRMVVRGDHTYAECCLTVVSRGTEAVVFSIDGSFTASVSIMGSDPKVRAGAVDVVRHWQDSGYLIVYVTGRPDMQKHRVVAWLSQHNFPHGVVSFCDGLTHDPLRQKAMFLQSLVQEVELNIVAGYGSPKDVAVYAALGLSPSQTYIVGRAVRKLQAQCQFLSDGYVAHLGQLEAGSHSHAPSGPPRAALAKSSYAVAAPVDFLRKQSQLLRSRGPSQVDREGPGTPPTTLARGKTRSISLKLDSEE

Target ORF information:

RefSeq Version	NM_008851.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphatidylinositol transfer protein, membrane-associated 1 (Pitpnm1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008851.4

ORF Insert Sequence:

ATGCTTATCA AGGAGTACCA CATCCTGCTG CCCATGAGCC TGGACGAGTA TCAAGTGGCC 
CAGCTCTACA TGATCCAGAA AAAGAGCCGT GAGGAGTCTA GTGGTGAGGG CAGCGGCGTG 
GAGATCCTGG CCAACCGGCC CTACACAGAT GGGCCTGGAG GCAACGGGCA GTATACACAC 
AAGGTGTACC ACGTGGGCTC CCACATCCCA GGCTGGTTCC GGGCACTGTT ACCCAAGGCT 
GCTCTGCAGG TAGAAGAGGA ATCTTGGAAC GCTTACCCAT ATACCCGGAC ACGGTACACC 
TGCCCCTTTG TGGAGAAGTT CTCCATTGAG ATAGAGACCT ACTACTTGCC TGATGGGGGG 
CAGCAACCTA ACGTCTTCAA CCTGAGTGGG GCTGAGAGAA GACAGAGAAT CGTGGATACC 
ATCGACATCG TGCGGGATGC AGTGGCCCCA GGAGAATACA AAGCGGAAGA GGACCCTCGG 
CTGTACCGCT CAGCCAAGAC AGGCCGAGGG CCGCTGGCTG ATGACTGGGC ACGGACAGCG 
GCCCAGACAG GACCTCTCAT GTGTGCCTAT AAGCTGTGCA AGGTTGAATT CCGCTACTGG 
GGCATGCAGG CCAAGATTGA GCAGTTCATC CATGACGTAG GTCTGCGCAG GGTGATGCTT 
CGCGCCCATC GCCAGGCCTG GTGTTGGCAA GATGAGTGGA TAGAACTGAG CATGGCTGAC 
ATCCGGGCAC TGGAGGAGGA GACTGCACGC ATGCTAGCGC AGCGTATGGC TAAGTGCAAC 
ACTGGCAGTG AGGGACCAGA GGCTCAGACC CCTGGGAAAT CCAGCACTGA GGCCCGACCT 
GGGACCAGCA CTGCTGGCAC CCCTGATGGA CCTGAGGCCC CTCCCGGCCC CGACGCCTCC 
CCAGATGCCA GCTTTGGGAA GCAGTGGTCC TCATCCTCCC GTTCCTCCTA CTCATCCCAA 
CATGGAGGCG GCGTGTCTCC ACAGAGCTTG TCTGAGTGGC GCATGCAGAA CATTGCCCGA 
GACTCTGAGA ACAGCTCCGA GGAAGAATTC TTTGATGCCC ATGAAGGTTT CTCGGACAGT 
GATGAGGTCT TCCCCAAGGA GATGACCAAG TGGAATTCCA ATGATTTTAT CGATGCCTTT 
GCCTCCCCAA CCGAGGTGGA GGGGGTACCA GATCCTACAG TCATGGCTAC CAAAGGCATT 
GAAGATGGGG CCCGAGCTCC CAGGGACTCA GAGGGCCTAG ATGGAGCGGG GGATCTAGTG 
GTCGAGGCGT GCTCTGTGCA TGCCCTCTTC CTCATCCTGC ACAGCGGCAG CATCCTGGAC 
TCTGGCCCTG GAGACACCAA CTCCAAGCAG GCCGACGTGC AAACGCTGAG CACAGCCTTT 
GAGGCCGTCA CCCGAGTCCA TTTCCCCGAG GCCCTGGGTC ACGTGGCACT GCGGCTGGTG 
CCCTGTCCAC CCATTTGCGC GGCTGCCTAT GCTCTTGTCT CCAACCTGAG CCCCTACAGC 
CATGATGGGG ATAGCCTGTC CCGCTCCCAA GACCACATTC CGCTGGCTGC CCTGCCGCTC 
CTGGCCACCT CATCCTCTCG CTACCAGGGT GCCGTGGCCA CTGTCATCGC TCGCACCAAC 
CAGGCCTATG CAGCCTTCCT GCGCTCATCG GAGGGCACAG GTTTCTGCGG GCAGGTGGTG 
CTGATCGGAG ATGGTGTTGG TGGCATCCTG GGCTTTGATG CGCTCTGCCA CAGTGCCAGT 
GCAGGCCCAG GGAGTCGGGG CAGCAGCCGC CGTGGGAGCA TGAACAATGA GATGCTCTCC 
CCTGAAGTTG GTCCAGTACG GGACCCGCTG GCAGATGGGG TGGAGGTACT GGGTCGTGCT 
AGCCCAGAAC CCTCAGCCCT ACCTGCTCAG CGTACCTTCA GTGACATGGC CAATCCTGAT 
CCTGATGGCT CTCAGAACAG CCTCCAGGTA GCCTCCACAG CTACCTCCTC TGGGGAGCCC 
CGCCGTGCAA GCACAGCCTC CTGTCCACCT GCCAGTTCTG AGGCTCCTGA CGGCCCCACG 
AACGCTGCTC GCCTGGACTT CAAGGTCTCA GGCTTCTTCC TCTTTGGCTC CCCACTGGGC 
CTGGTGCTGG CTCTTCGCAA AACCGTGATG CCCGCCTTGG AGGTGGCTCA GCTGCGTCCA 
GCCTGCGAGC AGATCTACAA TCTCTTCCAT GCCGCTGACC CCTGTGCCTC CCGCCTGGAA 
CCCCTGCTGG CCCCCAAGTT CCAGGCCATC GCCCCCCTGG CTGTACCTCG TTACCAGAAG 
TTTCCCCTAG GAGACGGCTC GTCCTTGCTG CTGGCTGACA CTTTGCAAAC CCATTCGAGT 
CTCTTCCTGG AAGAGCTGGA GATGATGGTG CCCTCCACAC CCACCTCGGC CAGTGGTGCC 
TTCTGGAAGG GCAGTGAACT AGGCAATGAG CCAGCATCCC AGACAGCAGC CCCCAGCACC 
ACCAGCGAGG TTGTTAAGAT CCTGGACCGC TGGTGGGGAA ACAAGCGGAT CGACTATTCA 
CTGTACTGCC CCGAGGCACT CACGGCCTTC CCCACGGTCA CGCTGCCCCA CCTCTTCCAC 
GCCAGCTACT GGGAATCAGC GGATGTAGTG GCCTTCATTC TGCGCCAGGT CATTGAGAAG 
GAGCGGCCAC AGCTGACAGA GTGTGAGGAG CCATCCATCT ATAGCCCCGC CTTCCCCAGG 
GAGAAGTGGC AGCGCAAACG CACACAGGTC AAGATCCGGA ACGTCACTTC CAACCACCGA 
GCGAGTGACA CCGTGGTATG TGAGGGCCGT CCCCAGGTGC TGAATGGGCG CTTCATGTAT 
GGACCATTGG ATGTGGTCAC GCTCACTGGA GAGAAGGTGG ATGTCTACGT CATGACACAG 
CCACTGTCAG GCAAGTGGAT CCACTTTGGC ACAGAGGTCA CTAACAGCTC AGGCCGTCTC 
ACCTTCCCAG TGCCCTCAGA ACGTGCACTG GGCATTGGTG TCTACCCTGT GCGCATGGTG 
GTCAGGGGAG ACCACACCTA TGCCGAGTGC TGTCTGACTG TGGTGTCCCG AGGCACAGAA 
GCTGTGGTCT TCAGCATCGA TGGTTCTTTC ACTGCCAGTG TCTCTATCAT GGGCAGCGAC 
CCCAAGGTGC GCGCTGGCGC AGTGGACGTG GTCAGGCACT GGCAGGACTC TGGCTACTTG 
ATTGTGTATG TAACTGGCCG GCCTGACATG CAAAAGCACC GTGTAGTGGC CTGGCTGTCG 
CAACACAACT TCCCCCATGG TGTTGTCTCC TTCTGTGATG GCCTCACCCA CGACCCGCTG 
CGGCAGAAAG CCATGTTTCT GCAGAGCCTG GTGCAAGAGG TAGAACTGAA CATCGTGGCT 
GGATATGGGT CACCAAAAGA TGTGGCAGTG TATGCAGCAC TGGGGCTCTC TCCGAGCCAG 
ACCTACATTG TTGGCCGTGC TGTGCGCAAA CTGCAGGCAC AGTGTCAGTT CCTGTCAGAT 
GGCTATGTGG CCCACTTGGG CCAGCTGGAG GCAGGCTCCC ACTCCCATGC TCCCTCAGGA 
CCTCCGAGAG CCGCTCTGGC CAAGAGTAGC TATGCTGTGG CTGCCCCTGT GGACTTCCTC 
CGGAAGCAGA GCCAGCTGCT TCGATCCAGA GGCCCTAGCC AGGTGGACCG TGAGGGTCCA 
GGAACACCTC CCACCACCCT GGCCAGGGGC AAGACTCGCA GCATCAGCCT CAAGTTGGAC 
AGTGAAGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pitpnm1 ( XM_017318081.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173570.1 Mus musculus phosphatidylinositol transfer protein, membrane-associated 1 (Pitpnm1), transcript variant X1, mRNA.

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CloneID	OMu79901
Clone ID Related Accession (Same CDS sequence)	XM_017318081.1
Accession Version	XM_017318081.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	membrane-associated phosphatidylinositol transfer protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTATCA AGGAGTACCA CATCCTGCTG CCCATGAGCC TGGACGAGTA TCAAGTGGCC 
CAGCTCTACA TGATCCAGAA AAAGAGCCGT GAGGAGTCTA GTGGTGAGGG CAGCGGCGTG 
GAGATCCTGG CCAACCGGCC CTACACAGAT GGGCCTGGAG GCAACGGGCA GTATACACAC 
AAGGTGTACC ACGTGGGCTC CCACATCCCA GGCTGGTTCC GGGCACTGTT ACCCAAGGCT 
GCTCTGCAGG TAGAAGAGGA ATCTTGGAAC GCTTACCCAT ATACCCGGAC ACGGTACACC 
TGCCCCTTTG TGGAGAAGTT CTCCATTGAG ATAGAGACCT ACTACTTGCC TGATGGGGGG 
CAGCAACCTA ACGTCTTCAA CCTGAGTGGG GCTGAGAGAA GACAGAGAAT CGTGGATACC 
ATCGACATCG TGCGGGATGC AGTGGCCCCA GGAGAATACA AAGCGGAAGA GGACCCTCGG 
CTGTACCGCT CAGCCAAGAC AGGCCGAGGG CCGCTGGCTG ATGACTGGGC ACGGACAGCG 
GCCCAGACAG GACCTCTCAT GTGTGCCTAT AAGCTGTGCA AGGTTGAATT CCGCTACTGG 
GGCATGCAGG CCAAGATTGA GCAGTTCATC CATGACGTAG GTCTGCGCAG GGTGATGCTT 
CGCGCCCATC GCCAGGCCTG GTGTTGGCAA GATGAGTGGA TAGAACTGAG CATGGCTGAC 
ATCCGGGCAC TGGAGGAGGA GACTGCACGC ATGCTAGCGC AGCGTATGGC TAAGTGCAAC 
ACTGGCAGTG AGGGACCAGA GGCTCAGACC CCTGGGAAAT CCAGCACTGA GGCCCGACCT 
GGGACCAGCA CTGCTGGCAC CCCTGATGGA CCTGAGGCCC CTCCCGGCCC CGACGCCTCC 
CCAGATGCCA GCTTTGGGAA GCAGTGGTCC TCATCCTCCC GTTCCTCCTA CTCATCCCAA 
CATGGAGGCG GCGTGTCTCC ACAGAGCTTG TCTGAGTGGC GCATGCAGAA CATTGCCCGA 
GACTCTGAGA ACAGCTCCGA GGAAGAATTC TTTGATGCCC ATGAAGGTTT CTCGGACAGT 
GATGAGGTCT TCCCCAAGGA GATGACCAAG TGGAATTCCA ATGATTTTAT CGATGCCTTT 
GCCTCCCCAA CCGAGGTGGA GGGGGTACCA GATCCTACAG TCATGGCTAC CAAAGGCATT 
GAAGATGGGG CCCGAGCTCC CAGGGACTCA GAGGGCCTAG ATGGAGCGGG GGATCTAGTG 
GTCGAGGCGT GCTCTGTGCA TGCCCTCTTC CTCATCCTGC ACAGCGGCAG CATCCTGGAC 
TCTGGCCCTG GAGACACCAA CTCCAAGCAG GCCGACGTGC AAACGCTGAG CACAGCCTTT 
GAGGCCGTCA CCCGAGTCCA TTTCCCCGAG GCCCTGGGTC ACGTGGCACT GCGGCTGGTG 
CCCTGTCCAC CCATTTGCGC GGCTGCCTAT GCTCTTGTCT CCAACCTGAG CCCCTACAGC 
CATGATGGGG ATAGCCTGTC CCGCTCCCAA GACCACATTC CGCTGGCTGC CCTGCCGCTC 
CTGGCCACCT CATCCTCTCG CTACCAGGGT GCCGTGGCCA CTGTCATCGC TCGCACCAAC 
CAGGCCTATG CAGCCTTCCT GCGCTCATCG GAGGGCACAG GTTTCTGCGG GCAGGTGGTG 
CTGATCGGAG ATGGTGTTGG TGGCATCCTG GGCTTTGATG CGCTCTGCCA CAGTGCCAGT 
GCAGGCCCAG GGAGTCGGGG CAGCAGCCGC CGTGGGAGCA TGAACAATGA GATGCTCTCC 
CCTGAAGTTG GTCCAGTACG GGACCCGCTG GCAGATGGGG TGGAGGTACT GGGTCGTGCT 
AGCCCAGAAC CCTCAGCCCT ACCTGCTCAG CGTACCTTCA GTGACATGGC CAATCCTGAT 
CCTGATGGCT CTCAGAACAG CCTCCAGGTA GCCTCCACAG CTACCTCCTC TGGGGAGCCC 
CGCCGTGCAA GCACAGCCTC CTGTCCACCT GCCAGTTCTG AGGCTCCTGA CGGCCCCACG 
AACGCTGCTC GCCTGGACTT CAAGGTCTCA GGCTTCTTCC TCTTTGGCTC CCCACTGGGC 
CTGGTGCTGG CTCTTCGCAA AACCGTGATG CCCGCCTTGG AGGTGGCTCA GCTGCGTCCA 
GCCTGCGAGC AGATCTACAA TCTCTTCCAT GCCGCTGACC CCTGTGCCTC CCGCCTGGAA 
CCCCTGCTGG CCCCCAAGTT CCAGGCCATC GCCCCCCTGG CTGTACCTCG TTACCAGAAG 
TTTCCCCTAG GAGACGGCTC GTCCTTGCTG CTGGCTGACA CTTTGCAAAC CCATTCGAGT 
CTCTTCCTGG AAGAGCTGGA GATGATGGTG CCCTCCACAC CCACCTCGGC CAGTGGTGCC 
TTCTGGAAGG GCAGTGAACT AGGCAATGAG CCAGCATCCC AGACAGCAGC CCCCAGCACC 
ACCAGCGAGG TTGTTAAGAT CCTGGACCGC TGGTGGGGAA ACAAGCGGAT CGACTATTCA 
CTGTACTGCC CCGAGGCACT CACGGCCTTC CCCACGGTCA CGCTGCCCCA CCTCTTCCAC 
GCCAGCTACT GGGAATCAGC GGATGTAGTG GCCTTCATTC TGCGCCAGGT CATTGAGAAG 
GAGCGGCCAC AGCTGACAGA GTGTGAGGAG CCATCCATCT ATAGCCCCGC CTTCCCCAGG 
GAGAAGTGGC AGCGCAAACG CACACAGGTC AAGATCCGGA ACGTCACTTC CAACCACCGA 
GCGAGTGACA CCGTGGTATG TGAGGGCCGT CCCCAGGTGC TGAATGGGCG CTTCATGTAT 
GGACCATTGG ATGTGGTCAC GCTCACTGGA GAGAAGGTGG ATGTCTACGT CATGACACAG 
CCACTGTCAG GCAAGTGGAT CCACTTTGGC ACAGAGGTCA CTAACAGCTC AGGCCGTCTC 
ACCTTCCCAG TGCCCTCAGA ACGTGCACTG GGCATTGGTG TCTACCCTGT GCGCATGGTG 
GTCAGGGGAG ACCACACCTA TGCCGAGTGC TGTCTGACTG TGGTGTCCCG AGGCACAGAA 
GCTGTGGTCT TCAGCATCGA TGGTTCTTTC ACTGCCAGTG TCTCTATCAT GGGCAGCGAC 
CCCAAGGTGC GCGCTGGCGC AGTGGACGTG GTCAGGCACT GGCAGGACTC TGGCTACTTG 
ATTGTGTATG TAACTGGCCG GCCTGACATG CAAAAGCACC GTGTAGTGGC CTGGCTGTCG 
CAACACAACT TCCCCCATGG TGTTGTCTCC TTCTGTGATG GCCTCACCCA CGACCCGCTG 
CGGCAGAAAG CCATGTTTCT GCAGAGCCTG GTGCAAGAGG GATACCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173570.1
CDS	245..3595
Translation	MLIKEYHILLPMSLDEYQVAQLYMIQKKSREESSGEGSGVEILANRPYTDGPGGNGQYTHKVYHVGSHIPGWFRALLPKAALQVEEESWNAYPYTRTRYTCPFVEKFSIEIETYYLPDGGQQPNVFNLSGAERRQRIVDTIDIVRDAVAPGEYKAEEDPRLYRSAKTGRGPLADDWARTAAQTGPLMCAYKLCKVEFRYWGMQAKIEQFIHDVGLRRVMLRAHRQAWCWQDEWIELSMADIRALEEETARMLAQRMAKCNTGSEGPEAQTPGKSSTEARPGTSTAGTPDGPEAPPGPDASPDASFGKQWSSSSRSSYSSQHGGGVSPQSLSEWRMQNIARDSENSSEEEFFDAHEGFSDSDEVFPKEMTKWNSNDFIDAFASPTEVEGVPDPTVMATKGIEDGARAPRDSEGLDGAGDLVVEACSVHALFLILHSGSILDSGPGDTNSKQADVQTLSTAFEAVTRVHFPEALGHVALRLVPCPPICAAAYALVSNLSPYSHDGDSLSRSQDHIPLAALPLLATSSSRYQGAVATVIARTNQAYAAFLRSSEGTGFCGQVVLIGDGVGGILGFDALCHSASAGPGSRGSSRRGSMNNEMLSPEVGPVRDPLADGVEVLGRASPEPSALPAQRTFSDMANPDPDGSQNSLQVASTATSSGEPRRASTASCPPASSEAPDGPTNAARLDFKVSGFFLFGSPLGLVLALRKTVMPALEVAQLRPACEQIYNLFHAADPCASRLEPLLAPKFQAIAPLAVPRYQKFPLGDGSSLLLADTLQTHSSLFLEELEMMVPSTPTSASGAFWKGSELGNEPASQTAAPSTTSEVVKILDRWWGNKRIDYSLYCPEALTAFPTVTLPHLFHASYWESADVVAFILRQVIEKERPQLTECEEPSIYSPAFPREKWQRKRTQVKIRNVTSNHRASDTVVCEGRPQVLNGRFMYGPLDVVTLTGEKVDVYVMTQPLSGKWIHFGTEVTNSSGRLTFPVPSERALGIGVYPVRMVVRGDHTYAECCLTVVSRGTEAVVFSIDGSFTASVSIMGSDPKVRAGAVDVVRHWQDSGYLIVYVTGRPDMQKHRVVAWLSQHNFPHGVVSFCDGLTHDPLRQKAMFLQSLVQEGYP

Target ORF information:

RefSeq Version	XM_017318081.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphatidylinositol transfer protein, membrane-associated 1 (Pitpnm1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318081.1

ORF Insert Sequence:

ATGCTTATCA AGGAGTACCA CATCCTGCTG CCCATGAGCC TGGACGAGTA TCAAGTGGCC 
CAGCTCTACA TGATCCAGAA AAAGAGCCGT GAGGAGTCTA GTGGTGAGGG CAGCGGCGTG 
GAGATCCTGG CCAACCGGCC CTACACAGAT GGGCCTGGAG GCAACGGGCA GTATACACAC 
AAGGTGTACC ACGTGGGCTC CCACATCCCA GGCTGGTTCC GGGCACTGTT ACCCAAGGCT 
GCTCTGCAGG TAGAAGAGGA ATCTTGGAAC GCTTACCCAT ATACCCGGAC ACGGTACACC 
TGCCCCTTTG TGGAGAAGTT CTCCATTGAG ATAGAGACCT ACTACTTGCC TGATGGGGGG 
CAGCAACCTA ACGTCTTCAA CCTGAGTGGG GCTGAGAGAA GACAGAGAAT CGTGGATACC 
ATCGACATCG TGCGGGATGC AGTGGCCCCA GGAGAATACA AAGCGGAAGA GGACCCTCGG 
CTGTACCGCT CAGCCAAGAC AGGCCGAGGG CCGCTGGCTG ATGACTGGGC ACGGACAGCG 
GCCCAGACAG GACCTCTCAT GTGTGCCTAT AAGCTGTGCA AGGTTGAATT CCGCTACTGG 
GGCATGCAGG CCAAGATTGA GCAGTTCATC CATGACGTAG GTCTGCGCAG GGTGATGCTT 
CGCGCCCATC GCCAGGCCTG GTGTTGGCAA GATGAGTGGA TAGAACTGAG CATGGCTGAC 
ATCCGGGCAC TGGAGGAGGA GACTGCACGC ATGCTAGCGC AGCGTATGGC TAAGTGCAAC 
ACTGGCAGTG AGGGACCAGA GGCTCAGACC CCTGGGAAAT CCAGCACTGA GGCCCGACCT 
GGGACCAGCA CTGCTGGCAC CCCTGATGGA CCTGAGGCCC CTCCCGGCCC CGACGCCTCC 
CCAGATGCCA GCTTTGGGAA GCAGTGGTCC TCATCCTCCC GTTCCTCCTA CTCATCCCAA 
CATGGAGGCG GCGTGTCTCC ACAGAGCTTG TCTGAGTGGC GCATGCAGAA CATTGCCCGA 
GACTCTGAGA ACAGCTCCGA GGAAGAATTC TTTGATGCCC ATGAAGGTTT CTCGGACAGT 
GATGAGGTCT TCCCCAAGGA GATGACCAAG TGGAATTCCA ATGATTTTAT CGATGCCTTT 
GCCTCCCCAA CCGAGGTGGA GGGGGTACCA GATCCTACAG TCATGGCTAC CAAAGGCATT 
GAAGATGGGG CCCGAGCTCC CAGGGACTCA GAGGGCCTAG ATGGAGCGGG GGATCTAGTG 
GTCGAGGCGT GCTCTGTGCA TGCCCTCTTC CTCATCCTGC ACAGCGGCAG CATCCTGGAC 
TCTGGCCCTG GAGACACCAA CTCCAAGCAG GCCGACGTGC AAACGCTGAG CACAGCCTTT 
GAGGCCGTCA CCCGAGTCCA TTTCCCCGAG GCCCTGGGTC ACGTGGCACT GCGGCTGGTG 
CCCTGTCCAC CCATTTGCGC GGCTGCCTAT GCTCTTGTCT CCAACCTGAG CCCCTACAGC 
CATGATGGGG ATAGCCTGTC CCGCTCCCAA GACCACATTC CGCTGGCTGC CCTGCCGCTC 
CTGGCCACCT CATCCTCTCG CTACCAGGGT GCCGTGGCCA CTGTCATCGC TCGCACCAAC 
CAGGCCTATG CAGCCTTCCT GCGCTCATCG GAGGGCACAG GTTTCTGCGG GCAGGTGGTG 
CTGATCGGAG ATGGTGTTGG TGGCATCCTG GGCTTTGATG CGCTCTGCCA CAGTGCCAGT 
GCAGGCCCAG GGAGTCGGGG CAGCAGCCGC CGTGGGAGCA TGAACAATGA GATGCTCTCC 
CCTGAAGTTG GTCCAGTACG GGACCCGCTG GCAGATGGGG TGGAGGTACT GGGTCGTGCT 
AGCCCAGAAC CCTCAGCCCT ACCTGCTCAG CGTACCTTCA GTGACATGGC CAATCCTGAT 
CCTGATGGCT CTCAGAACAG CCTCCAGGTA GCCTCCACAG CTACCTCCTC TGGGGAGCCC 
CGCCGTGCAA GCACAGCCTC CTGTCCACCT GCCAGTTCTG AGGCTCCTGA CGGCCCCACG 
AACGCTGCTC GCCTGGACTT CAAGGTCTCA GGCTTCTTCC TCTTTGGCTC CCCACTGGGC 
CTGGTGCTGG CTCTTCGCAA AACCGTGATG CCCGCCTTGG AGGTGGCTCA GCTGCGTCCA 
GCCTGCGAGC AGATCTACAA TCTCTTCCAT GCCGCTGACC CCTGTGCCTC CCGCCTGGAA 
CCCCTGCTGG CCCCCAAGTT CCAGGCCATC GCCCCCCTGG CTGTACCTCG TTACCAGAAG 
TTTCCCCTAG GAGACGGCTC GTCCTTGCTG CTGGCTGACA CTTTGCAAAC CCATTCGAGT 
CTCTTCCTGG AAGAGCTGGA GATGATGGTG CCCTCCACAC CCACCTCGGC CAGTGGTGCC 
TTCTGGAAGG GCAGTGAACT AGGCAATGAG CCAGCATCCC AGACAGCAGC CCCCAGCACC 
ACCAGCGAGG TTGTTAAGAT CCTGGACCGC TGGTGGGGAA ACAAGCGGAT CGACTATTCA 
CTGTACTGCC CCGAGGCACT CACGGCCTTC CCCACGGTCA CGCTGCCCCA CCTCTTCCAC 
GCCAGCTACT GGGAATCAGC GGATGTAGTG GCCTTCATTC TGCGCCAGGT CATTGAGAAG 
GAGCGGCCAC AGCTGACAGA GTGTGAGGAG CCATCCATCT ATAGCCCCGC CTTCCCCAGG 
GAGAAGTGGC AGCGCAAACG CACACAGGTC AAGATCCGGA ACGTCACTTC CAACCACCGA 
GCGAGTGACA CCGTGGTATG TGAGGGCCGT CCCCAGGTGC TGAATGGGCG CTTCATGTAT 
GGACCATTGG ATGTGGTCAC GCTCACTGGA GAGAAGGTGG ATGTCTACGT CATGACACAG 
CCACTGTCAG GCAAGTGGAT CCACTTTGGC ACAGAGGTCA CTAACAGCTC AGGCCGTCTC 
ACCTTCCCAG TGCCCTCAGA ACGTGCACTG GGCATTGGTG TCTACCCTGT GCGCATGGTG 
GTCAGGGGAG ACCACACCTA TGCCGAGTGC TGTCTGACTG TGGTGTCCCG AGGCACAGAA 
GCTGTGGTCT TCAGCATCGA TGGTTCTTTC ACTGCCAGTG TCTCTATCAT GGGCAGCGAC 
CCCAAGGTGC GCGCTGGCGC AGTGGACGTG GTCAGGCACT GGCAGGACTC TGGCTACTTG 
ATTGTGTATG TAACTGGCCG GCCTGACATG CAAAAGCACC GTGTAGTGGC CTGGCTGTCG 
CAACACAACT TCCCCCATGG TGTTGTCTCC TTCTGTGATG GCCTCACCCA CGACCCGCTG 
CGGCAGAAAG CCATGTTTCT GCAGAGCCTG GTGCAAGAGG GATACCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The lipid-transfer protein Nir2 enhances epithelial-mesenchymal transition and facilitates breast cancer metastasis.
Journal of cell science127(Pt 21)4740-9(2014 Nov)
Keinan O,Kedan A,Gavert N,Selitrennik M,Kim S,Karn T,Becker S,Lev S

Pitpnm1 is expressed in hair cells during development but is not required for hearing.
Neuroscience248620-5(2013 Sep)
Carlisle FA,Pearson S,Steel KP,Lewis MA

Ezh2, the histone methyltransferase of PRC2, regulates the balance between self-renewal and differentiation in the cerebral cortex.
Proceedings of the National Academy of Sciences of the United States of America107(36)15957-62(2010 Sep)
Pereira JD,Sansom SN,Smith J,Dobenecker MW,Tarakhovsky A,Livesey FJ

Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations.
Molecular & cellular proteomics : MCP6(2)283-93(2007 Feb)
Munton RP,Tweedie-Cullen R,Livingstone-Zatchej M,Weinandy F,Waidelich M,Longo D,Gehrig P,Potthast F,Rutishauser D,Gerrits B,Panse C,Schlapbach R,Mansuy IM

Substrate specificity of RdgB protein, a deoxyribonucleoside triphosphate pyrophosphohydrolase.
The Journal of biological chemistry282(6)3531-8(2007 Feb)
Burgis NE,Cunningham RP

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Pitpnm1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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