Ndufv1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ndufv1
Entrez Gene ID	17995
Full Name	NADH dehydrogenase (ubiquinone) flavoprotein 1
Synonyms	CI-51kD
General protein information	Preferred Names NADH dehydrogenase (ubiquinone) flavoprotein 1 Names NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial NADH dehydrogenase flavoprotein 1; 24 kDa (FP) NADH-ubiquinone oxidoreductase 51 kDa subunit complex I-51kD
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19 3.72 cM\|19 A
Summary	This gene encodes a subunit of the NADH-ubiquinone oxidoreductase (complex I) enzyme, which is a large, multimeric protein. It is the first enzyme complex in the mitochondrial electron transport chain and catalyzes the transfer of electrons from NADH to the electron acceptor ubiquinone. The proton gradient created by electron transfer drives the conversion of ADP to ATP. This gene is a core subunit and is conserved in prokaryotes and eukaryotes. The human ortholog of this protein has been characterized. It has consensus motifs for NADH, flavin mononucleotide, and iron-sulfur binding sites and participates in the oxidation of NADH as part of the dehydrogenase module of complex I. In humans, deficiencies in complex I are associated with myopathies, encephalomyopathies, and neurodegenerative disorders. [provided by RefSeq, Jun 2013].

mRNA and Protein(s)

mRNA	Protein	Name
NM_133666.3	NP_598427.1	NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	NDUFV1	NP_009034.2
Pan troglodytes (chimpanzee)	NDUFV1	NP_001065293.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010039	XP_319184.4
Caenorhabditis elegans (roundworm)	nuo-1	NP_496376.1
Danio rerio (zebrafish)	ndufv1	NP_001003747.1
Magnaporthe oryzae (rice blast fungus)	MGG_00647	XP_003718370.1
Oryza sativa (rice)	Os07g0645400	NP_001060455.1
Canis lupus familiaris (dog)	NDUFV1	XP_851761.2
Bos taurus (cattle)	NDUFV1	NP_777233.1
Rattus norvegicus (Norway rat)	Ndufv1	NP_001006973.1
Drosophila melanogaster (fruit fly)	CG9140	NP_608987.1
Arabidopsis thaliana (thale cress)	CI51	NP_196470.1
Mus musculus (house mouse)	Ndufv1	NP_598427.1
Neurospora crassa	NCU04044	XP_957659.1
Gallus gallus (chicken)	NDUFV1	NP_001026754.1
Xenopus tropicalis (tropical clawed frog)	ndufv1	NP_001011491.1

Gene Ontology
Bibliography

Related articles in PubMed

Lmx1a and Lmx1b regulate mitochondrial functions and survival of adult midbrain dopaminergic neurons.
Doucet-Beaupr? H, Gilbert C, Profes MS, Chabrat A, Pacelli C, Gigu?re N, Rioux V, Charest J, Deng Q, Laguna A, Ericson J, Perlmann T, Ang SL, Cicchetti F, Parent M, Trudeau LE, L?vesque M
Proceedings of the National Academy of Sciences of the United States of America113(30)E4387-96(2016 07)

Chronic Activation of ?2 AMPK Induces Obesity and Reduces ? Cell Function.
Yavari A, Stocker CJ, Ghaffari S, Wargent ET, Steeples V, Czibik G, Pinter K, Bellahcene M, Woods A, Mart?nez de Morentin PB, Cansell C, Lam BY, Chuster A, Petkevicius K, Nguyen-Tu MS, Martinez-Sanchez A, Pullen TJ, Oliver PL, Stockenhuber A, Nguyen C, Lazdam M, O'Dowd JF, Harikumar P, T?th M, Beall C, Kyriakou T, Parnis J, Sarma D, Katritsis G, Wortmann DD, Harper AR, Brown LA, Willows R, Gandra S, Poncio V, de Oliveira Figueiredo MJ, Qi NR, Peirson SN, McCrimmon RJ, Gereben B, Tretter L, Fekete C, Redwood C, Yeo GS, Heisler LK, Rutter GA, Smith MA, Withers DJ, Carling D, Sternick EB, Arch JR, Cawthorne MA, Watkins H, Ashrafian H
Cell metabolism23(5)821-36(2016 May)

Mitochondrial complex I deficiency enhances skeletal myogenesis but impairs insulin signaling through SIRT1 inactivation.
Hong J, Kim BW, Choo HJ, Park JJ, Yi JS, Yu DM, Lee H, Yoon GS, Lee JS, Ko YG
The Journal of biological chemistry289(29)20012-25(2014 Jul)

MCJ/DnaJC15, an endogenous mitochondrial repressor of the respiratory chain that controls metabolic alterations.
Hatle KM, Gummadidala P, Navasa N, Bernardo E, Dodge J, Silverstrim B, Fortner K, Burg E, Suratt BT, Hammer J, Radermacher M, Taatjes DJ, Thornton T, Anguita J, Rincon M
Molecular and cellular biology33(11)2302-14(2013 Jun)

Subunit-specific incorporation efficiency and kinetics in mitochondrial complex I homeostasis.
Dieteren CE, Koopman WJ, Swarts HG, Peters JG, Maczuga P, van Gemst JJ, Masereeuw R, Smeitink JA, Nijtmans LG, Willems PH
The Journal of biological chemistry287(50)41851-60(2012 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NDUFV1 knockdown-induced blockage of insulin signaling was released by protein-tyrosine phosphatase 1B knockdown in C2C12 myotubes, and NDUFV1 or SIRT1 knockdown did not affect mitochondria biogenesis during C2C12 myogenesis. myogenesis.
Title: Mitochondrial complex I deficiency enhances skeletal myogenesis but impairs insulin signaling through SIRT1 inactivation.

The authors use green fluorescent-tagged complex I subunits to determine the kinetics of assembly. Tagged human NDUFV1 can replace endogenous protein in the holo-complex I protein and was also found in complex I subassemblies, suggesting that NDUFV1 may directly incorporate into the holo-enzyme and may also assemble into smaller complexes or in a monomer pool.
Title: Subunit-specific incorporation efficiency and kinetics in mitochondrial complex I homeostasis.

The authors provide an overview of the function and assembly of human complex I, also known as NADH dehydrogenase (ubiquinone) 1 alpha subcomplex. They discuss its role in oxidative phosphorylation and the implications of complex I deficiency in disease.
Title: Molecular base of biochemical complex I deficiency.

The authors clone and characterize the human NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial protein, also known as NDUFV1. They identify transcription factor binding sites as well as consensus motifs for NADH, flavin mononucleotide, and iron-sulfur binding sites. The human ortholog has high homology to the cow NDUFV1 protein.
Title: Cloning of the human mitochondrial 51 kDa subunit (NDUFV1) reveals a 100% antisense homology of its 3'UTR with the 5'UTR of the gamma-interferon inducible protein (IP-30) precursor: is this a link between mitochondrial myopathy and inflammation?

The following Ndufv1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ndufv1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu16457	NM_133666.3 Latest version!	Mus musculus NADH:ubiquinone oxidoreductase core subunit V1 (Ndufv1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ndufv1 ( NM_133666.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598427.1 Mus musculus NADH:ubiquinone oxidoreductase core subunit V1 (Ndufv1), mRNA.

Price & Availability↑

CloneID	OMu16457
Clone ID Related Accession (Same CDS sequence)	NM_133666.3
Accession Version	NM_133666.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Mus musculus(house mouse)
Product	NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE863504.1, AK075692.1, AA223049.1 and AW113957.1. On Jun 4, 2013 this sequence version replaced NM_133666.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC014818.1, AK005021.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 23038253; reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTAGCGG CACGGCATTT TCTCGGCGGG TTGGTTCCCG TGCGGGTATC TGTGCGTTTC 
AGCAGCGGCA CGACAGCACC CAAGAAAACC TCATTTGGCT CACTGAAGGA TGAAGACCGG 
ATCTTTACCA ACCTGTATGG GCGCCATGAC TGGAGGCTGA AAGGTGCCCT GAGACGGGGT 
GACTGGTACA AGACAAAGGA GATCCTGCTG AAGGGGCCTG ACTGGATCTT GGGTGAGATG 
AAGACATCAG GTTTACGGGG CCGTGGTGGT GCTGGCTTCC CCACTGGCCT CAAGTGGAGC 
TTCATGAATA AGCCCTCAGA TGGCAGGCCC AAGTATCTGG TGGTGAATGC TGACGAGGGA 
GAGCCAGGCA CCTGTAAGGA CCGAGAGATC ATGCGTCATG ACCCTCACAA GCTGGTGGAA 
GGCTGCCTTG TGGGAGGCCG GGCCATGGGA GCCCGGGCCG CCTATATCTA CATCCGAGGG 
GAATTCTACA ACGAGGCCTC CAATTTGCAG GTAGCTATCC GAGAGGCCTA TGAAGCAGGT 
CTGATTGGCA AGAATGCTTG TGGCTCCGAC TATGATTTTG ATGTGTTTGT GGTGCGTGGG 
GCTGGGGCCT ACATCTGTGG AGAAGAGACG GCACTTATTG AATCCATTGA AGGCAAGCAG 
GGAAAGCCAC GCCTGAAGCC GCCCTTTCCA GCAGATGTGG GAGTGTTTGG ATGCCCCACA 
ACTGTGGCCA ATGTGGAGAC AGTGGCTGTG TCCCCCACCA TTTGCCGTCG TGGGGGCACC 
TGGTTTGCTG GCTTTGGCCG AGAACGCAAT TCAGGTACCA AATTGTTTAA CATCTCTGGC 
CATGTCAACC ACCCCTGCAC TGTGGAGGAA GAGATGTCTG TGCCACTGAA AGAGCTGATT 
GAGAAACATG CTGGTGGTGT CACAGGTGGC TGGGACAACC TCCTTGCTGT GATTCCTGGT 
GGCTCATCTA CTCCACTGAT TCCCAAATCT GTGTGTGAGA CCGTGCTAAT GGACTTCGAT 
GCACTGGTGC AGGCTCAGAC AGGCCTGGGC ACGGCTGCAG TTATTGTTAT GGATCGCTCG 
ACAGACATTG TGAAAGCCAT CGCTCGTCTC ATTGAGTTCT ACAAGCATGA GAGCTGTGGC 
CAGTGTACCC CGTGCCGTGA GGGCGTTGAC TGGATGAACA AGGTGATGGC CCGATTTGTG 
AAGGGAGATG CCCGGCCAGC TGAGATTGAC TCCCTGTGGG AGATCAGCAA GCAGATAGAA 
GGCCACACCA TTTGTGCTCT GGGTGATGGG GCTGCCTGGC CAGTACAGGG TCTGATCCGA 
CATTTCAGGC CAGAGCTTGA GGATCGGATG CAACGATTTG CCCAGCAGCA CCGGGCCTGG 
CAGGCAGCCT CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598427.1
CDS	134..1528
Translation	MLAARHFLGGLVPVRVSVRFSSGTTAPKKTSFGSLKDEDRIFTNLYGRHDWRLKGALRRGDWYKTKEILLKGPDWILGEMKTSGLRGRGGAGFPTGLKWSFMNKPSDGRPKYLVVNADEGEPGTCKDREIMRHDPHKLVEGCLVGGRAMGARAAYIYIRGEFYNEASNLQVAIREAYEAGLIGKNACGSDYDFDVFVVRGAGAYICGEETALIESIEGKQGKPRLKPPFPADVGVFGCPTTVANVETVAVSPTICRRGGTWFAGFGRERNSGTKLFNISGHVNHPCTVEEEMSVPLKELIEKHAGGVTGGWDNLLAVIPGGSSTPLIPKSVCETVLMDFDALVQAQTGLGTAAVIVMDRSTDIVKAIARLIEFYKHESCGQCTPCREGVDWMNKVMARFVKGDARPAEIDSLWEISKQIEGHTICALGDGAAWPVQGLIRHFRPELEDRMQRFAQQHRAWQAAS

Target ORF information:

RefSeq Version	NM_133666.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NADH:ubiquinone oxidoreductase core subunit V1 (Ndufv1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133666.3

ORF Insert Sequence:

ATGCTAGCGG CACGGCATTT TCTCGGCGGG TTGGTTCCCG TGCGGGTATC TGTGCGTTTC 
AGCAGCGGCA CGACAGCACC CAAGAAAACC TCATTTGGCT CACTGAAGGA TGAAGACCGG 
ATCTTTACCA ACCTGTATGG GCGCCATGAC TGGAGGCTGA AAGGTGCCCT GAGACGGGGT 
GACTGGTACA AGACAAAGGA GATCCTGCTG AAGGGGCCTG ACTGGATCTT GGGTGAGATG 
AAGACATCAG GTTTACGGGG CCGTGGTGGT GCTGGCTTCC CCACTGGCCT CAAGTGGAGC 
TTCATGAATA AGCCCTCAGA TGGCAGGCCC AAGTATCTGG TGGTGAATGC TGACGAGGGA 
GAGCCAGGCA CCTGTAAGGA CCGAGAGATC ATGCGTCATG ACCCTCACAA GCTGGTGGAA 
GGCTGCCTTG TGGGAGGCCG GGCCATGGGA GCCCGGGCCG CCTATATCTA CATCCGAGGG 
GAATTCTACA ACGAGGCCTC CAATTTGCAG GTAGCTATCC GAGAGGCCTA TGAAGCAGGT 
CTGATTGGCA AGAATGCTTG TGGCTCCGAC TATGATTTTG ATGTGTTTGT GGTGCGTGGG 
GCTGGGGCCT ACATCTGTGG AGAAGAGACG GCACTTATTG AATCCATTGA AGGCAAGCAG 
GGAAAGCCAC GCCTGAAGCC GCCCTTTCCA GCAGATGTGG GAGTGTTTGG ATGCCCCACA 
ACTGTGGCCA ATGTGGAGAC AGTGGCTGTG TCCCCCACCA TTTGCCGTCG TGGGGGCACC 
TGGTTTGCTG GCTTTGGCCG AGAACGCAAT TCAGGTACCA AATTGTTTAA CATCTCTGGC 
CATGTCAACC ACCCCTGCAC TGTGGAGGAA GAGATGTCTG TGCCACTGAA AGAGCTGATT 
GAGAAACATG CTGGTGGTGT CACAGGTGGC TGGGACAACC TCCTTGCTGT GATTCCTGGT 
GGCTCATCTA CTCCACTGAT TCCCAAATCT GTGTGTGAGA CCGTGCTAAT GGACTTCGAT 
GCACTGGTGC AGGCTCAGAC AGGCCTGGGC ACGGCTGCAG TTATTGTTAT GGATCGCTCG 
ACAGACATTG TGAAAGCCAT CGCTCGTCTC ATTGAGTTCT ACAAGCATGA GAGCTGTGGC 
CAGTGTACCC CGTGCCGTGA GGGCGTTGAC TGGATGAACA AGGTGATGGC CCGATTTGTG 
AAGGGAGATG CCCGGCCAGC TGAGATTGAC TCCCTGTGGG AGATCAGCAA GCAGATAGAA 
GGCCACACCA TTTGTGCTCT GGGTGATGGG GCTGCCTGGC CAGTACAGGG TCTGATCCGA 
CATTTCAGGC CAGAGCTTGA GGATCGGATG CAACGATTTG CCCAGCAGCA CCGGGCCTGG 
CAGGCAGCCT CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Lmx1a and Lmx1b regulate mitochondrial functions and survival of adult midbrain dopaminergic neurons.
Proceedings of the National Academy of Sciences of the United States of America113(30)E4387-96(2016 07)
Doucet-Beaupr? H,Gilbert C,Profes MS,Chabrat A,Pacelli C,Gigu?re N,Rioux V,Charest J,Deng Q,Laguna A,Ericson J,Perlmann T,Ang SL,Cicchetti F,Parent M,Trudeau LE,L?vesque M

Chronic Activation of ?2 AMPK Induces Obesity and Reduces ? Cell Function.
Cell metabolism23(5)821-36(2016 May)
Yavari A,Stocker CJ,Ghaffari S,Wargent ET,Steeples V,Czibik G,Pinter K,Bellahcene M,Woods A,Mart?nez de Morentin PB,Cansell C,Lam BY,Chuster A,Petkevicius K,Nguyen-Tu MS,Martinez-Sanchez A,Pullen TJ,Oliver PL,Stockenhuber A,Nguyen C,Lazdam M,O'Dowd JF,Harikumar P,T?th M,Beall C,Kyriakou T,Parnis J,Sarma D,Katritsis G,Wortmann DD,Harper AR,Brown LA,Willows R,Gandra S,Poncio V,de Oliveira Figueiredo MJ,Qi NR,Peirson SN,McCrimmon RJ,Gereben B,Tretter L,Fekete C,Redwood C,Yeo GS,Heisler LK,Rutter GA,Smith MA,Withers DJ,Carling D,Sternick EB,Arch JR,Cawthorne MA,Watkins H,Ashrafian H

Mitochondrial complex I deficiency enhances skeletal myogenesis but impairs insulin signaling through SIRT1 inactivation.
The Journal of biological chemistry289(29)20012-25(2014 Jul)
Hong J,Kim BW,Choo HJ,Park JJ,Yi JS,Yu DM,Lee H,Yoon GS,Lee JS,Ko YG

MCJ/DnaJC15, an endogenous mitochondrial repressor of the respiratory chain that controls metabolic alterations.
Molecular and cellular biology33(11)2302-14(2013 Jun)
Hatle KM,Gummadidala P,Navasa N,Bernardo E,Dodge J,Silverstrim B,Fortner K,Burg E,Suratt BT,Hammer J,Radermacher M,Taatjes DJ,Thornton T,Anguita J,Rincon M

Subunit-specific incorporation efficiency and kinetics in mitochondrial complex I homeostasis.
The Journal of biological chemistry287(50)41851-60(2012 Dec)
Dieteren CE,Koopman WJ,Swarts HG,Peters JG,Maczuga P,van Gemst JJ,Masereeuw R,Smeitink JA,Nijtmans LG,Willems PH

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Ndufv1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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