Mdk cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mdk
Entrez Gene ID	17242
Full Name	midkine
Synonyms	MK,Mek
General protein information	Preferred Names midkine Names midkine retinoic acid-induced differentiation factor retinoic acid-responsive protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 E1\|2 50.63 cM
Summary	This gene encodes a secreted growth factor that belongs to the pleiotrophin/midkine heparin-binding protein family and functions in a variety of biological processes. The encoded cytokine promotes the growth, differentiation, survival and migration of several target cells including leucocytes involved in inflammation. This protein plays a role in the formation of scar tissue and intraperitoneal adhesions, and promotes neurite outgrowth and neuron survival. The protein encoded by this gene is associated with obesity and inhibition of insulin signaling in fat cells. A pseudogene of this gene is present on chromosome 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001291482.1	NP_001278411.1	midkine isoform b
NM_001012336.2	NP_001012336.1	midkine isoform a precursor
NM_001291481.1	NP_001278410.1	midkine isoform a precursor
NM_001291483.1	NP_001278412.1	midkine isoform c precursor
XM_011239335.2	XP_011237637.1	midkine isoform X1
NM_010784.5	NP_034914.1	midkine isoform a precursor
NM_001012335.2	NP_001012335.1	midkine isoform a precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	MDK	NP_002382.1
Danio rerio (zebrafish)	mdka	NP_571145.1
Danio rerio (zebrafish)	mdkb	NP_571791.1
Rattus norvegicus (Norway rat)	Mdk	NP_110486.1
	MDK	XP_002799613.1
Mus musculus (house mouse)	Mdk	NP_034914.1
Gallus gallus (chicken)	MDK	NP_001106760.1
Bos taurus (cattle)	MDK	NP_776360.2
Xenopus tropicalis (tropical clawed frog)	mdk	NP_989074.1

Gene Ontology
Bibliography

Related articles in PubMed

Midkine, a heparin-binding cytokine, plays key roles in intraperitoneal adhesions.
Inoh K, Muramatsu H, Ochiai K, Torii S, Muramatsu T
Biochemical and biophysical research communications317(1)108-13(2004 Apr)

cDNA cloning and sequencing of a new gene intensely expressed in early differentiation stages of embryonal carcinoma cells and in mid-gestation period of mouse embryogenesis.
Kadomatsu K, Tomomura M, Muramatsu T
Biochemical and biophysical research communications151(3)1312-8(1988 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MDK functions in the ventral tegmental area to limit ethanol consumption and levels of CCL2
Title: Midkine in the mouse ventral tegmental area limits ethanol intake and Ccl2 gene expression.

It may be involved in the regeneration of acinar cells in acute pancreatitis.
Title: Midkine is overexpressed in acute pancreatitis and promotes the pancreatic recovery in L-arginine-induced acute pancreatitis in mice.

Analyses of the melanoma secretome and validation in clinical specimens showed that the heparin-binding factor midkine is a systemic inducer of neo-lymphangiogenesis that defines patient prognosis. This role of midkine was linked to a paracrine activation of the mTOR pathway in lymphatic endothelial cells.
Title: Whole-body imaging of lymphovascular niches identifies pre-metastatic roles of midkine.

MK serves an indispensable role in the NFAT-regulated activation of CD4(+) T cells and Th1 cell differentiation.
Title: Growth Factor Midkine Promotes T-Cell Activation through Nuclear Factor of Activated T Cells Signaling and Th1 Cell Differentiation in Lupus Nephritis.

MK limits amphetamine-induced striatal neuroinflammation
Title: Midkine Is a Novel Regulator of Amphetamine-Induced Striatal Gliosis and Cognitive Impairment: Evidence for a Stimulus-Dependent Regulation of Neuroinflammation by Midkine.

The following Mdk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mdk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu20442	NM_001291482.1 Latest version!	Mus musculus midkine (Mdk), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu20442	XM_011239335.2 Latest version!	Mus musculus midkine (Mdk), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu20330	NM_001291483.1 Latest version!	Mus musculus midkine (Mdk), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu20530	NM_001012335.2 Latest version!	Mus musculus midkine (Mdk), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu20530	NM_001012336.2 Latest version!	Mus musculus midkine (Mdk), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu20530	NM_001291481.1 Latest version!	Mus musculus midkine (Mdk), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu20530	NM_010784.5 Latest version!	Mus musculus midkine (Mdk), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mdk ( NM_001291482.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001278411.1 Mus musculus midkine (Mdk), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OMu20442
Clone ID Related Accession (Same CDS sequence)	NM_001291482.1 , XM_011239335.2
Accession Version	NM_001291482.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	midkine isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY092625.1, AL731772.9, CJ174578.1 and CF728526.1. On Apr 18, 2014 this sequence version replaced XM_006498859.1. Transcript Variant: This variant (5) differs in the 5' UTR, lacks a portion of the 5' coding region and uses a downstream start codon compared to variant 1. The encoded isoform (b) has a shorter N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CJ174578.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164142 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCTTCC GCGAGGGTAC CTGTGGGGCC CAGACCCAGC GCGTCCATTG CAAGGTGCCC 
TGCAACTGGA AGAAGGAATT TGGAGCCGAC TGCAAATACA AGTTTGAGAG CTGGGGGGCG 
TGTGATGGGA GCACTGGCAC CAAAGCCCGC CAAGGGACCC TGAAGAAGGC GCGGTACAAT 
GCCCAGTGCC AGGAGACCAT CCGCGTGACT AAGCCCTGCA CCTCCAAGAC CAAGTCAAAG 
ACCAAAGCCA AGAAAGGAAA AGGAAAGGAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278411.1
CDS	241..513
Translation	MGFREGTCGAQTQRVHCKVPCNWKKEFGADCKYKFESWGACDGSTGTKARQGTLKKARYNAQCQETIRVTKPCTSKTKSKTKAKKGKGKD

Target ORF information:

RefSeq Version	NM_001291482.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus midkine (Mdk), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001291482.1

ORF Insert Sequence:

ATGGGCTTCC GCGAGGGTAC CTGTGGGGCC CAGACCCAGC GCGTCCATTG CAAGGTGCCC 
TGCAACTGGA AGAAGGAATT TGGAGCCGAC TGCAAATACA AGTTTGAGAG CTGGGGGGCG 
TGTGATGGGA GCACTGGCAC CAAAGCCCGC CAAGGGACCC TGAAGAAGGC GCGGTACAAT 
GCCCAGTGCC AGGAGACCAT CCGCGTGACT AAGCCCTGCA CCTCCAAGAC CAAGTCAAAG 
ACCAAAGCCA AGAAAGGAAA AGGAAAGGAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mdk ( XM_011239335.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237637.1 Mus musculus midkine (Mdk), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu20442
Clone ID Related Accession (Same CDS sequence)	NM_001291482.1 , XM_011239335.2
Accession Version	XM_011239335.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	midkine isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011239335.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTTCC GCGAGGGTAC CTGTGGGGCC CAGACCCAGC GCGTCCATTG CAAGGTGCCC 
TGCAACTGGA AGAAGGAATT TGGAGCCGAC TGCAAATACA AGTTTGAGAG CTGGGGGGCG 
TGTGATGGGA GCACTGGCAC CAAAGCCCGC CAAGGGACCC TGAAGAAGGC GCGGTACAAT 
GCCCAGTGCC AGGAGACCAT CCGCGTGACT AAGCCCTGCA CCTCCAAGAC CAAGTCAAAG 
ACCAAAGCCA AGAAAGGAAA AGGAAAGGAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237637.1
CDS	236..508
Translation	MGFREGTCGAQTQRVHCKVPCNWKKEFGADCKYKFESWGACDGSTGTKARQGTLKKARYNAQCQETIRVTKPCTSKTKSKTKAKKGKGKD

Target ORF information:

RefSeq Version	XM_011239335.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus midkine (Mdk), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011239335.2

ORF Insert Sequence:

ATGGGCTTCC GCGAGGGTAC CTGTGGGGCC CAGACCCAGC GCGTCCATTG CAAGGTGCCC 
TGCAACTGGA AGAAGGAATT TGGAGCCGAC TGCAAATACA AGTTTGAGAG CTGGGGGGCG 
TGTGATGGGA GCACTGGCAC CAAAGCCCGC CAAGGGACCC TGAAGAAGGC GCGGTACAAT 
GCCCAGTGCC AGGAGACCAT CCGCGTGACT AAGCCCTGCA CCTCCAAGAC CAAGTCAAAG 
ACCAAAGCCA AGAAAGGAAA AGGAAAGGAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mdk ( NM_001291483.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001278412.1 Mus musculus midkine (Mdk), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OMu20330
Clone ID Related Accession (Same CDS sequence)	NM_001291483.1
Accession Version	NM_001291483.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	midkine isoform c precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL731772.9. Transcript Variant: This variant (6) lacks an alternate in-frame exon in the central coding region compared to variant 1. The encoded isoform (c) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ323887.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164137 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGCACC GAGGCTTCTT CCTTCTCGCC CTTCTTGCCC TCTTGGTGGT CACGTCCGCG 
GTGGCCAAAA AAAAAGAGAA GGTGAAGAAG GGCAGCGAGT GTTCGGAGTG GACCTGGGGG 
CCCTGCACCC CCAGCAGCAA GGACTGCGGC ATGGGCTTCC GCGAGGGTAC CTGTGGGGCC 
CAGACCCAGC GCGTCCATTG CAAGGTGCCC TGCAACTGGA AGAAGGAATT TGGAGCCAAG 
AAAGGAAAAG GAAAGGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278412.1
CDS	370..630
Translation	MQHRGFFLLALLALLVVTSAVAKKKEKVKKGSECSEWTWGPCTPSSKDCGMGFREGTCGAQTQRVHCKVPCNWKKEFGAKKGKGKD

Target ORF information:

RefSeq Version	NM_001291483.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus midkine (Mdk), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001291483.1

ORF Insert Sequence:

ATGCAGCACC GAGGCTTCTT CCTTCTCGCC CTTCTTGCCC TCTTGGTGGT CACGTCCGCG 
GTGGCCAAAA AAAAAGAGAA GGTGAAGAAG GGCAGCGAGT GTTCGGAGTG GACCTGGGGG 
CCCTGCACCC CCAGCAGCAA GGACTGCGGC ATGGGCTTCC GCGAGGGTAC CTGTGGGGCC 
CAGACCCAGC GCGTCCATTG CAAGGTGCCC TGCAACTGGA AGAAGGAATT TGGAGCCAAG 
AAAGGAAAAG GAAAGGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mdk ( NM_001012336.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001012336.1 Mus musculus midkine (Mdk), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu20530
Clone ID Related Accession (Same CDS sequence)	NM_001012336.2 , NM_001291481.1 , NM_010784.5 , NM_001012335.2
Accession Version	NM_001012336.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	midkine isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY092625.1, AL731772.9, M34327.1 and CF728526.1. On Apr 18, 2014 this sequence version replaced NM_001012336.1. Transcript Variant: This variant (3, also known as MK2; PMID 2345177) differs in the 5' UTR compared to variant 1. Variants 1 through 4 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CB202501.1, CB204300.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN01164137 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCAGCACC GAGGCTTCTT CCTTCTCGCC CTTCTTGCCC TCTTGGTGGT CACGTCCGCG 
GTGGCCAAAA AAAAAGAGAA GGTGAAGAAG GGCAGCGAGT GTTCGGAGTG GACCTGGGGG 
CCCTGCACCC CCAGCAGCAA GGACTGCGGC ATGGGCTTCC GCGAGGGTAC CTGTGGGGCC 
CAGACCCAGC GCGTCCATTG CAAGGTGCCC TGCAACTGGA AGAAGGAATT TGGAGCCGAC 
TGCAAATACA AGTTTGAGAG CTGGGGGGCG TGTGATGGGA GCACTGGCAC CAAAGCCCGC 
CAAGGGACCC TGAAGAAGGC GCGGTACAAT GCCCAGTGCC AGGAGACCAT CCGCGTGACT 
AAGCCCTGCA CCTCCAAGAC CAAGTCAAAG ACCAAAGCCA AGAAAGGAAA AGGAAAGGAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001012336.1
CDS	168..590
Translation	MQHRGFFLLALLALLVVTSAVAKKKEKVKKGSECSEWTWGPCTPSSKDCGMGFREGTCGAQTQRVHCKVPCNWKKEFGADCKYKFESWGACDGSTGTKARQGTLKKARYNAQCQETIRVTKPCTSKTKSKTKAKKGKGKD

Target ORF information:

RefSeq Version	NM_001012336.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus midkine (Mdk), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001012336.2

ORF Insert Sequence:

ATGCAGCACC GAGGCTTCTT CCTTCTCGCC CTTCTTGCCC TCTTGGTGGT CACGTCCGCG 
GTGGCCAAAA AAAAAGAGAA GGTGAAGAAG GGCAGCGAGT GTTCGGAGTG GACCTGGGGG 
CCCTGCACCC CCAGCAGCAA GGACTGCGGC ATGGGCTTCC GCGAGGGTAC CTGTGGGGCC 
CAGACCCAGC GCGTCCATTG CAAGGTGCCC TGCAACTGGA AGAAGGAATT TGGAGCCGAC 
TGCAAATACA AGTTTGAGAG CTGGGGGGCG TGTGATGGGA GCACTGGCAC CAAAGCCCGC 
CAAGGGACCC TGAAGAAGGC GCGGTACAAT GCCCAGTGCC AGGAGACCAT CCGCGTGACT 
AAGCCCTGCA CCTCCAAGAC CAAGTCAAAG ACCAAAGCCA AGAAAGGAAA AGGAAAGGAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mdk ( NM_001291481.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001278410.1 Mus musculus midkine (Mdk), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu20530
Clone ID Related Accession (Same CDS sequence)	NM_001012336.2 , NM_001291481.1 , NM_010784.5 , NM_001012335.2
Accession Version	NM_001291481.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	midkine isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CJ174056.1 and CF728526.1. On Apr 18, 2014 this sequence version replaced XM_006498858.1. Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1 through 4 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CF728526.1, AV570726.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164137, SAMN01164138 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGCACC GAGGCTTCTT CCTTCTCGCC CTTCTTGCCC TCTTGGTGGT CACGTCCGCG 
GTGGCCAAAA AAAAAGAGAA GGTGAAGAAG GGCAGCGAGT GTTCGGAGTG GACCTGGGGG 
CCCTGCACCC CCAGCAGCAA GGACTGCGGC ATGGGCTTCC GCGAGGGTAC CTGTGGGGCC 
CAGACCCAGC GCGTCCATTG CAAGGTGCCC TGCAACTGGA AGAAGGAATT TGGAGCCGAC 
TGCAAATACA AGTTTGAGAG CTGGGGGGCG TGTGATGGGA GCACTGGCAC CAAAGCCCGC 
CAAGGGACCC TGAAGAAGGC GCGGTACAAT GCCCAGTGCC AGGAGACCAT CCGCGTGACT 
AAGCCCTGCA CCTCCAAGAC CAAGTCAAAG ACCAAAGCCA AGAAAGGAAA AGGAAAGGAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278410.1
CDS	78..500
Translation	MQHRGFFLLALLALLVVTSAVAKKKEKVKKGSECSEWTWGPCTPSSKDCGMGFREGTCGAQTQRVHCKVPCNWKKEFGADCKYKFESWGACDGSTGTKARQGTLKKARYNAQCQETIRVTKPCTSKTKSKTKAKKGKGKD

Target ORF information:

RefSeq Version	NM_001291481.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus midkine (Mdk), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001291481.1

ORF Insert Sequence:

ATGCAGCACC GAGGCTTCTT CCTTCTCGCC CTTCTTGCCC TCTTGGTGGT CACGTCCGCG 
GTGGCCAAAA AAAAAGAGAA GGTGAAGAAG GGCAGCGAGT GTTCGGAGTG GACCTGGGGG 
CCCTGCACCC CCAGCAGCAA GGACTGCGGC ATGGGCTTCC GCGAGGGTAC CTGTGGGGCC 
CAGACCCAGC GCGTCCATTG CAAGGTGCCC TGCAACTGGA AGAAGGAATT TGGAGCCGAC 
TGCAAATACA AGTTTGAGAG CTGGGGGGCG TGTGATGGGA GCACTGGCAC CAAAGCCCGC 
CAAGGGACCC TGAAGAAGGC GCGGTACAAT GCCCAGTGCC AGGAGACCAT CCGCGTGACT 
AAGCCCTGCA CCTCCAAGAC CAAGTCAAAG ACCAAAGCCA AGAAAGGAAA AGGAAAGGAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mdk ( NM_010784.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034914.1 Mus musculus midkine (Mdk), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu20530
Clone ID Related Accession (Same CDS sequence)	NM_001012336.2 , NM_001291481.1 , NM_010784.5 , NM_001012335.2
Accession Version	NM_010784.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	midkine isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL731772.9, M34328.1 and CF728526.1. On Apr 18, 2014 this sequence version replaced NM_010784.4. Transcript Variant: This variant (1, also known as MK3; PMID 2345177) represents the longest transcript and encodes the longest isoform (a). Variants 1 through 4 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BU515905.1, M34328.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN01164137 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGCACC GAGGCTTCTT CCTTCTCGCC CTTCTTGCCC TCTTGGTGGT CACGTCCGCG 
GTGGCCAAAA AAAAAGAGAA GGTGAAGAAG GGCAGCGAGT GTTCGGAGTG GACCTGGGGG 
CCCTGCACCC CCAGCAGCAA GGACTGCGGC ATGGGCTTCC GCGAGGGTAC CTGTGGGGCC 
CAGACCCAGC GCGTCCATTG CAAGGTGCCC TGCAACTGGA AGAAGGAATT TGGAGCCGAC 
TGCAAATACA AGTTTGAGAG CTGGGGGGCG TGTGATGGGA GCACTGGCAC CAAAGCCCGC 
CAAGGGACCC TGAAGAAGGC GCGGTACAAT GCCCAGTGCC AGGAGACCAT CCGCGTGACT 
AAGCCCTGCA CCTCCAAGAC CAAGTCAAAG ACCAAAGCCA AGAAAGGAAA AGGAAAGGAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034914.1
CDS	370..792
Translation	MQHRGFFLLALLALLVVTSAVAKKKEKVKKGSECSEWTWGPCTPSSKDCGMGFREGTCGAQTQRVHCKVPCNWKKEFGADCKYKFESWGACDGSTGTKARQGTLKKARYNAQCQETIRVTKPCTSKTKSKTKAKKGKGKD

Target ORF information:

RefSeq Version	NM_010784.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus midkine (Mdk), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010784.5

ORF Insert Sequence:

ATGCAGCACC GAGGCTTCTT CCTTCTCGCC CTTCTTGCCC TCTTGGTGGT CACGTCCGCG 
GTGGCCAAAA AAAAAGAGAA GGTGAAGAAG GGCAGCGAGT GTTCGGAGTG GACCTGGGGG 
CCCTGCACCC CCAGCAGCAA GGACTGCGGC ATGGGCTTCC GCGAGGGTAC CTGTGGGGCC 
CAGACCCAGC GCGTCCATTG CAAGGTGCCC TGCAACTGGA AGAAGGAATT TGGAGCCGAC 
TGCAAATACA AGTTTGAGAG CTGGGGGGCG TGTGATGGGA GCACTGGCAC CAAAGCCCGC 
CAAGGGACCC TGAAGAAGGC GCGGTACAAT GCCCAGTGCC AGGAGACCAT CCGCGTGACT 
AAGCCCTGCA CCTCCAAGAC CAAGTCAAAG ACCAAAGCCA AGAAAGGAAA AGGAAAGGAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mdk ( NM_001012335.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001012335.1 Mus musculus midkine (Mdk), transcript variant 2, mRNA.

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CloneID	OMu20530
Clone ID Related Accession (Same CDS sequence)	NM_001012336.2 , NM_001291481.1 , NM_010784.5 , NM_001012335.2
Accession Version	NM_001012335.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	midkine isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL731772.9, CF738400.1 and CF728526.1. On Apr 18, 2014 this sequence version replaced NM_001012335.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 through 4 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CF738400.1, AA222497.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164137, SAMN01164138 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGCACC GAGGCTTCTT CCTTCTCGCC CTTCTTGCCC TCTTGGTGGT CACGTCCGCG 
GTGGCCAAAA AAAAAGAGAA GGTGAAGAAG GGCAGCGAGT GTTCGGAGTG GACCTGGGGG 
CCCTGCACCC CCAGCAGCAA GGACTGCGGC ATGGGCTTCC GCGAGGGTAC CTGTGGGGCC 
CAGACCCAGC GCGTCCATTG CAAGGTGCCC TGCAACTGGA AGAAGGAATT TGGAGCCGAC 
TGCAAATACA AGTTTGAGAG CTGGGGGGCG TGTGATGGGA GCACTGGCAC CAAAGCCCGC 
CAAGGGACCC TGAAGAAGGC GCGGTACAAT GCCCAGTGCC AGGAGACCAT CCGCGTGACT 
AAGCCCTGCA CCTCCAAGAC CAAGTCAAAG ACCAAAGCCA AGAAAGGAAA AGGAAAGGAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001012335.1
CDS	171..593
Translation	MQHRGFFLLALLALLVVTSAVAKKKEKVKKGSECSEWTWGPCTPSSKDCGMGFREGTCGAQTQRVHCKVPCNWKKEFGADCKYKFESWGACDGSTGTKARQGTLKKARYNAQCQETIRVTKPCTSKTKSKTKAKKGKGKD

Target ORF information:

RefSeq Version	NM_001012335.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus midkine (Mdk), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001012335.2

ORF Insert Sequence:

ATGCAGCACC GAGGCTTCTT CCTTCTCGCC CTTCTTGCCC TCTTGGTGGT CACGTCCGCG 
GTGGCCAAAA AAAAAGAGAA GGTGAAGAAG GGCAGCGAGT GTTCGGAGTG GACCTGGGGG 
CCCTGCACCC CCAGCAGCAA GGACTGCGGC ATGGGCTTCC GCGAGGGTAC CTGTGGGGCC 
CAGACCCAGC GCGTCCATTG CAAGGTGCCC TGCAACTGGA AGAAGGAATT TGGAGCCGAC 
TGCAAATACA AGTTTGAGAG CTGGGGGGCG TGTGATGGGA GCACTGGCAC CAAAGCCCGC 
CAAGGGACCC TGAAGAAGGC GCGGTACAAT GCCCAGTGCC AGGAGACCAT CCGCGTGACT 
AAGCCCTGCA CCTCCAAGAC CAAGTCAAAG ACCAAAGCCA AGAAAGGAAA AGGAAAGGAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Midkine, a heparin-binding cytokine, plays key roles in intraperitoneal adhesions.
Biochemical and biophysical research communications317(1)108-13(2004 Apr)
Inoh K,Muramatsu H,Ochiai K,Torii S,Muramatsu T

cDNA cloning and sequencing of a new gene intensely expressed in early differentiation stages of embryonal carcinoma cells and in mid-gestation period of mouse embryogenesis.
Biochemical and biophysical research communications151(3)1312-8(1988 Mar)
Kadomatsu K,Tomomura M,Muramatsu T

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Mdk cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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