H1f8 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	H1f8
Entrez Gene ID	171506
Full Name	H1 histone family, member O, oocyte-specific
Synonyms	C86609,H1.8,H1fo,H1oo
General protein information	Preferred Names H1 histone family, member O, oocyte-specific Names histone H1oo oocyte-specific histone H1 oocyte-specific linker histone H1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6\|6 E3
Summary	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. The protein encoded is a replication-independent histone that is a member of the histone H1 family. This gene contains introns, unlike most histone genes and the encoded protein is expressed only in oocytes. [provided by RefSeq, Oct 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001346702.1	NP_001333631.1	histone H1oo isoform 2
NM_138311.3	NP_612184.1	histone H1oo isoform 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	H1foo	NP_612184.1
	LOC702823	XP_001094128.1
Pan troglodytes (chimpanzee)	H1FOO	XP_001144407.1
Rattus norvegicus (Norway rat)	H1foo	NP_001102821.1
Homo sapiens (human)	H1FOO	NP_722575.1
Canis lupus familiaris (dog)	H1FOO	XP_852262.2
Bos taurus (cattle)	H1FOO	NP_001030449.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

H1foo Has a Pivotal Role in Qualifying Induced Pluripotent Stem Cells.
Kunitomi A, Yuasa S, Sugiyama F, Saito Y, Seki T, Kusumoto D, Kashimura S, Takei M, Tohyama S, Hashimoto H, Egashira T, Tanimoto Y, Mizuno S, Tanaka S, Okuno H, Yamazawa K, Watanabe H, Oda M, Kaneda R, Matsuzaki Y, Nagai T, Okano H, Yagami K, Tanaka M, Fukuda K
Stem cell reports6(6)825-33(2016 06)

Role of H1 linker histones in mammalian development and stem cell differentiation.
Pan C, Fan Y
Biochimica et biophysica acta1859(3)496-509(2016 Mar)

Histone variants: key players of chromatin.
Biterge B, Schneider R
Cell and tissue research356(3)457-66(2014 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

H1foo contributes to the generation of higher-quality induced pluripotent stem cells.
Title: H1foo Has a Pivotal Role in Qualifying Induced Pluripotent Stem Cells.

H1foo has an impact on the genome-wide, locus-specific epigenetic status.
Title: Oocyte-specific linker histone H1foo is an epigenomic modulator that decondenses chromatin and impairs pluripotency.

Promoter activities of three mouse maternal genes (H1oo, Npm2, and Zar1) in oocytes and pre-implantation embryos, and examined the promoters and to obttain the best promoter for oocytte gene expression.
Title: Cis-acting elements (E-box and NBE) in the promoter region of three maternal genes (Histone H1oo, Nucleoplasmin 2, and Zygote Arrest 1) are required for oocyte-specific gene expression in the mouse.

The unmethylated status was continuously observed throughout oogenesis, in mature oocytes, and after fertilization, in E3.5 blastocysts. There is a continuously unmethylated circuit at the H1foo locus in the female germline.
Title: DNA hypomethylation circuit of the mouse oocyte-specific histone H1foo gene in female germ cell lineage.

that H1foo is essential for maturation of germinal vesicle-stage oocytes
Title: H1foo is indispensable for meiotic maturation of the mouse oocyte.

The following H1f8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the H1f8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu21803	NM_138311.3 Latest version!	Mus musculus H1.8 linker histone (H1f8), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu36968	NM_001346702.1 Latest version!	Mus musculus H1.8 linker histone (H1f8), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

H1f8 ( NM_138311.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_612184.1 Mus musculus H1.8 linker histone (H1f8), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu21803
Clone ID Related Accession (Same CDS sequence)	NM_138311.3
Accession Version	NM_138311.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	histone H1oo isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CJ081178.1, AK135944.1 and AV357657.1. On Jun 8, 2015 this sequence version replaced NM_138311.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK135944.1, AY007195.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## replication-independent histone :: PMID: 24781148 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTCCTG GGAGTGTCTC CAGTGTTTCT TCCTCCTCTT TTCCCTCCAG GGACACATCC 
CCTTCTGGAT CATGTGGGCT CCCTGGAGCT GACAAGCCAG GTCCAAGTTG CCGCAGAATC 
CAAGCAGGCC AAAGGAACCC AACAATGCTG CACATGGTGC TAGAGGCTTT GAAGGCCCGG 
GAGGCACGCC AGGGCACATC AGTTGTAGCC ATCAAGGTCT ACATCCAACA CAAGTACCCG 
ACAGTGGACA CCACCCGTTT CAAGTACCTG TTGAAGCAAG CTCTGGAAAC TGGCGTTCGT 
CGAGGCCTCC TCACCAGGCC TGCTCACTCC AAGGCCAAGG GTGCCACTGG CAGCTTCAAA 
CTAGTTCCAA AGCCCAAGAC AAAGAAAGCC TGTGCCCCCA AAGCCGGCAG GGGAGCTGCA 
GGTGCCAAGG AGACAGGCTC CAAGAAATCT GGATTGCTGA AGAAAGACCA AGTTGGCAAG 
GCCACGATGG AGAAAGGGCA GAAGAGGAGG GCTTACCCTT GCAAGGCAGC CACACTGGAG 
ATGGCACCTA AGAAAGCCAA GGCGAAACCG AAAGAGGTCA GAAAGGCTCC CCTAAAACAA 
GACAAAGCAG CAGGGGCCCC TCTGACTGCC AATGGAGGCC AGAAGGTCAA ACGCAGTGGG 
AGCAGGCAAG AAGCAAATGC CCATGGGAAA ACCAAAGGTG AGAAATCGAA GCCCTTGGCC 
AGCAAGGTCC AGAATAGCGT TGCTTCCCTC GCCAAAAGGA AGATGGCAGA CATGGCCCAC 
ACTGTGACAG TTGTTCAGGG GGCTGAGACA GTACAGGAGA CCAAAGTGCC CACTCCTTCC 
CAGGACATAG GACACAAAGT ACAACCCATA CCTAGGGTCA GGAAGGCAAA GACCCCTGAG 
AACACTCAGG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_612184.1
CDS	53..967
Translation	MAPGSVSSVSSSSFPSRDTSPSGSCGLPGADKPGPSCRRIQAGQRNPTMLHMVLEALKAREARQGTSVVAIKVYIQHKYPTVDTTRFKYLLKQALETGVRRGLLTRPAHSKAKGATGSFKLVPKPKTKKACAPKAGRGAAGAKETGSKKSGLLKKDQVGKATMEKGQKRRAYPCKAATLEMAPKKAKAKPKEVRKAPLKQDKAAGAPLTANGGQKVKRSGSRQEANAHGKTKGEKSKPLASKVQNSVASLAKRKMADMAHTVTVVQGAETVQETKVPTPSQDIGHKVQPIPRVRKAKTPENTQA

Target ORF information:

RefSeq Version	NM_138311.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus H1.8 linker histone (H1f8), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138311.3

ORF Insert Sequence:

ATGGCTCCTG GGAGTGTCTC CAGTGTTTCT TCCTCCTCTT TTCCCTCCAG GGACACATCC 
CCTTCTGGAT CATGTGGGCT CCCTGGAGCT GACAAGCCAG GTCCAAGTTG CCGCAGAATC 
CAAGCAGGCC AAAGGAACCC AACAATGCTG CACATGGTGC TAGAGGCTTT GAAGGCCCGG 
GAGGCACGCC AGGGCACATC AGTTGTAGCC ATCAAGGTCT ACATCCAACA CAAGTACCCG 
ACAGTGGACA CCACCCGTTT CAAGTACCTG TTGAAGCAAG CTCTGGAAAC TGGCGTTCGT 
CGAGGCCTCC TCACCAGGCC TGCTCACTCC AAGGCCAAGG GTGCCACTGG CAGCTTCAAA 
CTAGTTCCAA AGCCCAAGAC AAAGAAAGCC TGTGCCCCCA AAGCCGGCAG GGGAGCTGCA 
GGTGCCAAGG AGACAGGCTC CAAGAAATCT GGATTGCTGA AGAAAGACCA AGTTGGCAAG 
GCCACGATGG AGAAAGGGCA GAAGAGGAGG GCTTACCCTT GCAAGGCAGC CACACTGGAG 
ATGGCACCTA AGAAAGCCAA GGCGAAACCG AAAGAGGTCA GAAAGGCTCC CCTAAAACAA 
GACAAAGCAG CAGGGGCCCC TCTGACTGCC AATGGAGGCC AGAAGGTCAA ACGCAGTGGG 
AGCAGGCAAG AAGCAAATGC CCATGGGAAA ACCAAAGGTG AGAAATCGAA GCCCTTGGCC 
AGCAAGGTCC AGAATAGCGT TGCTTCCCTC GCCAAAAGGA AGATGGCAGA CATGGCCCAC 
ACTGTGACAG TTGTTCAGGG GGCTGAGACA GTACAGGAGA CCAAAGTGCC CACTCCTTCC 
CAGGACATAG GACACAAAGT ACAACCCATA CCTAGGGTCA GGAAGGCAAA GACCCCTGAG 
AACACTCAGG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

H1f8 ( NM_001346702.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001333631.1 Mus musculus H1.8 linker histone (H1f8), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu36968
Clone ID Related Accession (Same CDS sequence)	NM_001346702.1
Accession Version	NM_001346702.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	histone H1oo isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DV655349.1, AK162154.1 and AC142099.3. On Oct 26, 2016 this sequence version replaced XM_006505678.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK162154.1, BC145046.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## replication-independent histone :: PMID: 24781148 ##RefSeq-Attributes-END##

ATGGCTCCTG GGAGTGTCTC CAGTGTTTCT TCCTCCTCTT TTCCCTCCAG GGACACATCC 
CCTTCTGGAT CATGTGGGCT CCCTGGAGCT GACAAGCCAG GTCCAAGTTG CCGCAGAATC 
CAAGCAGGCC AAAGGAACCC AACAATGCTG CACATGGTGC TAGAGGCTTT GAAGGCCCGG 
GAGGCACGCC AGGGCACATC AGTTGTAGCC ATCAAGGTCT ACATCCAACA CAAGTACCCG 
ACAGTGGACA CCACCCGTTT CAAGTACCTG TTGAAGCAAG CTCTGGAAAC TGGCGTTCGT 
CGAGGCCTCC TCACCAGGCC TGCTCACTCC AAGGCCAAGG GTGCCACTGG CAGCTTCAAA 
CTAGTTCCAA AGCCCAAGAC AAAGAAAGCC TGTGCCCCCA AAGCCGGCAG GGGAGCTGCA 
GGTGCCAAGG AGACAGGCTC CAAGAAATCT GGATTGCTGA AGAAAGACCA AGTTGGCAAG 
GCCACGATGG AGAAAGGGCA GAAGAGGAGG GCTTACCCTT GCAAGGCAGC CACACTGGAG 
ATGGCACCTA AGAAAGCCAA GGCGAAACCG AAAGAGGTCA GAAAGGCTCC CCTAAAACAA 
GACAAAGCAG CAGGGGCCCC TCTGACTGCC AATGGAGGCC AGAAGGTCAA ACGCAGTGGG 
AGCAGGCAAG AAGCAAATGC CCATGGGAAA ACCAAAGGTG AGAAATCGAA GCCCTTGGCC 
AGCAAGGTAG GTCCAGAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333631.1
CDS	53..793
Translation	MAPGSVSSVSSSSFPSRDTSPSGSCGLPGADKPGPSCRRIQAGQRNPTMLHMVLEALKAREARQGTSVVAIKVYIQHKYPTVDTTRFKYLLKQALETGVRRGLLTRPAHSKAKGATGSFKLVPKPKTKKACAPKAGRGAAGAKETGSKKSGLLKKDQVGKATMEKGQKRRAYPCKAATLEMAPKKAKAKPKEVRKAPLKQDKAAGAPLTANGGQKVKRSGSRQEANAHGKTKGEKSKPLASKVGPE

Target ORF information:

RefSeq Version	NM_001346702.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus H1.8 linker histone (H1f8), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346702.1

ORF Insert Sequence:

ATGGCTCCTG GGAGTGTCTC CAGTGTTTCT TCCTCCTCTT TTCCCTCCAG GGACACATCC 
CCTTCTGGAT CATGTGGGCT CCCTGGAGCT GACAAGCCAG GTCCAAGTTG CCGCAGAATC 
CAAGCAGGCC AAAGGAACCC AACAATGCTG CACATGGTGC TAGAGGCTTT GAAGGCCCGG 
GAGGCACGCC AGGGCACATC AGTTGTAGCC ATCAAGGTCT ACATCCAACA CAAGTACCCG 
ACAGTGGACA CCACCCGTTT CAAGTACCTG TTGAAGCAAG CTCTGGAAAC TGGCGTTCGT 
CGAGGCCTCC TCACCAGGCC TGCTCACTCC AAGGCCAAGG GTGCCACTGG CAGCTTCAAA 
CTAGTTCCAA AGCCCAAGAC AAAGAAAGCC TGTGCCCCCA AAGCCGGCAG GGGAGCTGCA 
GGTGCCAAGG AGACAGGCTC CAAGAAATCT GGATTGCTGA AGAAAGACCA AGTTGGCAAG 
GCCACGATGG AGAAAGGGCA GAAGAGGAGG GCTTACCCTT GCAAGGCAGC CACACTGGAG 
ATGGCACCTA AGAAAGCCAA GGCGAAACCG AAAGAGGTCA GAAAGGCTCC CCTAAAACAA 
GACAAAGCAG CAGGGGCCCC TCTGACTGCC AATGGAGGCC AGAAGGTCAA ACGCAGTGGG 
AGCAGGCAAG AAGCAAATGC CCATGGGAAA ACCAAAGGTG AGAAATCGAA GCCCTTGGCC 
AGCAAGGTAG GTCCAGAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

H1foo Has a Pivotal Role in Qualifying Induced Pluripotent Stem Cells.
Stem cell reports6(6)825-33(2016 06)
Kunitomi A,Yuasa S,Sugiyama F,Saito Y,Seki T,Kusumoto D,Kashimura S,Takei M,Tohyama S,Hashimoto H,Egashira T,Tanimoto Y,Mizuno S,Tanaka S,Okuno H,Yamazawa K,Watanabe H,Oda M,Kaneda R,Matsuzaki Y,Nagai T,Okano H,Yagami K,Tanaka M,Fukuda K

Role of H1 linker histones in mammalian development and stem cell differentiation.
Biochimica et biophysica acta1859(3)496-509(2016 Mar)
Pan C,Fan Y

Histone variants: key players of chromatin.
Cell and tissue research356(3)457-66(2014 Jun)
Biterge B,Schneider R

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H1f8 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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