P3h3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	P3h3
Entrez Gene ID	14789
Full Name	prolyl 3-hydroxylase 3
Synonyms	BC016431,Grcb,Leprel2
General protein information	Preferred Names prolyl 3-hydroxylase 3 Names prolyl 3-hydroxylase 3 gene rich cluster, B leprecan-like 2 leprecan-like protein 2 protein B
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6 F2\|6 59.17 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_017321394.1	XP_017176883.1	prolyl 3-hydroxylase 3 isoform X1
XM_030255169.1	XP_030111029.1	prolyl 3-hydroxylase 3 isoform X1
NM_013534.4	NP_038562.2	prolyl 3-hydroxylase 3 precursor
NM_013534.5	NP_038562.2	prolyl 3-hydroxylase 3 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LEPREL2	XP_003313512.2
Canis lupus familiaris (dog)	LEPREL2	XP_003433588.2
Mus musculus (house mouse)	Leprel2	NP_038562.2
Gallus gallus (chicken)	LEPREL2	XP_416512.4
Bos taurus (cattle)	LEPREL2	XP_003586189.2
Homo sapiens (human)	LEPREL2	NP_055077.2
	LOC713865	XP_001110328.2
Rattus norvegicus (Norway rat)	Leprel2	NP_001100090.2
Danio rerio (zebrafish)	leprel2	XP_696266.3

Gene Ontology
Bibliography

Related articles in PubMed

Cellular heterogeneity in the ureteric progenitor niche and distinct profiles of branching morphogenesis in organ development.
Rutledge EA, Benazet JD, McMahon AP
Development (Cambridge, England)144(17)3177-3188(2017 09)

P3h3-null and Sc65-null Mice Phenocopy the Collagen Lysine Under-hydroxylation and Cross-linking Abnormality of Ehlers-Danlos Syndrome Type VIA.
Hudson DM, Weis M, Rai J, Joeng KS, Dimori M, Lee BH, Morello R, Eyre DR
The Journal of biological chemistry292(9)3877-3887(2017 03)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Sc65-Null Mice Provide Evidence for a Novel Endoplasmic Reticulum Complex Regulating Collagen Lysyl Hydroxylation.
Heard ME, Besio R, Weis M, Rai J, Hudson DM, Dimori M, Zimmerman SM, Kamykowski JA, Hogue WR, Swain FL, Burdine MS, Mackintosh SG, Tackett AJ, Suva LJ, Eyre DR, Morello R
PLoS genetics12(4)e1006002(2016 Apr)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

both P3h3- and Sc65-null mice displayed a similar pattern of severe under-hydroxylation of lysines, particularly at cross-linking sites in type I collagen from bone, skin, dentin, cornea, and aorta, revealing a complex evolution and interplay between the proteins forming the machinery of collagen prolyl 3-hydroxylation and lysyl hydroxylation in the ER
Title: P3h3-null and Sc65-null Mice Phenocopy the Collagen Lysine Under-hydroxylation and Cross-linking Abnormality of Ehlers-Danlos Syndrome Type VIA.

This report characterizes expression of the P3H1, P3H2 and P3H3 genes in embryonic and adult mice.
Title: Expression of prolyl 3-hydroxylase genes in embryonic and adult mouse tissues.

Study found that all 3 mouse Leprecan genes Lepre1, leprel1 and leprel2 are expressed in the notochord; additionally, they are expressed in dorsal root ganglia, limb buds, branchial arches, and developing kidneys.
Title: Conservation of notochord gene expression across chordates: insights from the Leprecan gene family.

The following P3h3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the P3h3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu81644	XM_017321394.1 Latest version!	Mus musculus prolyl 3-hydroxylase 3 (P3h3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu89129	XM_030255169.1 Latest version!	Mus musculus prolyl 3-hydroxylase 3 (P3h3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu09556	NM_013534.5 Latest version!	Mus musculus prolyl 3-hydroxylase 3 (P3h3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$433.30 $619.00
View Old Accession Versions >>
OMu09556	NM_013534.4	Mus musculus prolyl 3-hydroxylase 3 (P3h3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$433.30 $619.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

P3h3 ( XM_017321394.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176883.1 Mus musculus prolyl 3-hydroxylase 3 (P3h3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu81644
Clone ID Related Accession (Same CDS sequence)	XM_017321394.1
Accession Version	XM_017321394.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1218bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	prolyl 3-hydroxylase 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGG CCATGGAAAA TGTCCTGAGT GTCCTGCTCT TCTACCCAGA GGATGAGGCT 
GCCAAAAAGG CTCTGAACCA GTACCAAACT CAGTTGGGAG AGCCAAGACC TGACCTGGGA 
CCACGAGAGG ACATCCAGCG GTTCATCCTT CGCTCCCTCG GAGAGAAGAG ACAGTTATAT 
TACGCCATGG AGCATCTGGG CACTAGCTTC AAGGACCCGG ATTCTTGGAC CCCGGAGGCT 
CTCATCCCTA AGGCACTGAG AGAGAGGCTC AGAGAGGATC AAGAGAAGAA GCCCTGGGAC 
CATCAGCCTC CACAGCAGAA GCCCTTGGCT CACTGGAAGG ATGCCCTTCT GATGGAGGGT 
GTGACCCTCA CGCAGGACGC CCAGCAGCTG AATGGGTCGG AGCGAGCTGT CTTAGATGGG 
CTGCTAACTT CAGCCGAGTG TGGGGTTCTG CTGCAGCTGG CCAAGGATGC AGCACAGGCT 
GGAGCCAGGT CAGGCTACCG TGGCCGCCGC TCTCCTCATA GTCCTCATGA ACGCTTTGAG 
GGGCTCACGG TGCTCAAGGC TGCTCAGCTG GCCCGGGCAG GGACTGTGGG CAGGCCAGGC 
GCTAAGCTGC TTCTGGAGGT AAGTGAGCGA GTGCGGACTT TGACCCAGGC CTACTTCTCC 
CCGGAACGGC CACTGCATCT TTCCTTCACC CACCTGGTGT GCCGAAGTGC CATAGAAGGT 
GAACAAGAGC AGCGTATGGA CCTGAGTCAC CCGGTTCATG CAGACAACTG TGTCCTGGAC 
CCCGACACTG GCGAATGCTG GCGGGAGCCC CCAGCCTACA CCTATCGAGA CTATAGTGGA 
CTCCTCTACC TCAATGATGA CTTCAAGGGA GGCGACCTGT TCTTCACGCA GCCCAACGCC 
CTCACTGTCA CGGCTCAGGT CCGTCCTCGG TGTGGGCGCC TTGTGGCCTT CAGCTCTGGT 
GGTGAGAATC CCCATGGTGT ATGGGCTGTG ACTCGGGGAC GGCGCTGTGC CCTAGCACTG 
TGGCACACGT GGGCACCTGA GCACAGTGAA CAGGAGTGGA CAGAAGCCAA AGAGCTGCTG 
CAGGAGGAAG AGGAGGAAGA AGAGGAGGAA GACATTCTCA GCAGAGACCC TTCCCCAGAA 
CCCCCAAGTC ACAAGCTTCA GCGAGTCCAG GAGAAAGCTG GGAAGCCCCG CCGGGTCCGG 
GTCCGAGAGG AACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176883.1
CDS	756..1973
Translation	MSQAMENVLSVLLFYPEDEAAKKALNQYQTQLGEPRPDLGPREDIQRFILRSLGEKRQLYYAMEHLGTSFKDPDSWTPEALIPKALRERLREDQEKKPWDHQPPQQKPLAHWKDALLMEGVTLTQDAQQLNGSERAVLDGLLTSAECGVLLQLAKDAAQAGARSGYRGRRSPHSPHERFEGLTVLKAAQLARAGTVGRPGAKLLLEVSERVRTLTQAYFSPERPLHLSFTHLVCRSAIEGEQEQRMDLSHPVHADNCVLDPDTGECWREPPAYTYRDYSGLLYLNDDFKGGDLFFTQPNALTVTAQVRPRCGRLVAFSSGGENPHGVWAVTRGRRCALALWHTWAPEHSEQEWTEAKELLQEEEEEEEEEDILSRDPSPEPPSHKLQRVQEKAGKPRRVRVREEL

Target ORF information:

RefSeq Version	XM_017321394.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus prolyl 3-hydroxylase 3 (P3h3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321394.1

ORF Insert Sequence:

ATGTCTCAGG CCATGGAAAA TGTCCTGAGT GTCCTGCTCT TCTACCCAGA GGATGAGGCT 
GCCAAAAAGG CTCTGAACCA GTACCAAACT CAGTTGGGAG AGCCAAGACC TGACCTGGGA 
CCACGAGAGG ACATCCAGCG GTTCATCCTT CGCTCCCTCG GAGAGAAGAG ACAGTTATAT 
TACGCCATGG AGCATCTGGG CACTAGCTTC AAGGACCCGG ATTCTTGGAC CCCGGAGGCT 
CTCATCCCTA AGGCACTGAG AGAGAGGCTC AGAGAGGATC AAGAGAAGAA GCCCTGGGAC 
CATCAGCCTC CACAGCAGAA GCCCTTGGCT CACTGGAAGG ATGCCCTTCT GATGGAGGGT 
GTGACCCTCA CGCAGGACGC CCAGCAGCTG AATGGGTCGG AGCGAGCTGT CTTAGATGGG 
CTGCTAACTT CAGCCGAGTG TGGGGTTCTG CTGCAGCTGG CCAAGGATGC AGCACAGGCT 
GGAGCCAGGT CAGGCTACCG TGGCCGCCGC TCTCCTCATA GTCCTCATGA ACGCTTTGAG 
GGGCTCACGG TGCTCAAGGC TGCTCAGCTG GCCCGGGCAG GGACTGTGGG CAGGCCAGGC 
GCTAAGCTGC TTCTGGAGGT AAGTGAGCGA GTGCGGACTT TGACCCAGGC CTACTTCTCC 
CCGGAACGGC CACTGCATCT TTCCTTCACC CACCTGGTGT GCCGAAGTGC CATAGAAGGT 
GAACAAGAGC AGCGTATGGA CCTGAGTCAC CCGGTTCATG CAGACAACTG TGTCCTGGAC 
CCCGACACTG GCGAATGCTG GCGGGAGCCC CCAGCCTACA CCTATCGAGA CTATAGTGGA 
CTCCTCTACC TCAATGATGA CTTCAAGGGA GGCGACCTGT TCTTCACGCA GCCCAACGCC 
CTCACTGTCA CGGCTCAGGT CCGTCCTCGG TGTGGGCGCC TTGTGGCCTT CAGCTCTGGT 
GGTGAGAATC CCCATGGTGT ATGGGCTGTG ACTCGGGGAC GGCGCTGTGC CCTAGCACTG 
TGGCACACGT GGGCACCTGA GCACAGTGAA CAGGAGTGGA CAGAAGCCAA AGAGCTGCTG 
CAGGAGGAAG AGGAGGAAGA AGAGGAGGAA GACATTCTCA GCAGAGACCC TTCCCCAGAA 
CCCCCAAGTC ACAAGCTTCA GCGAGTCCAG GAGAAAGCTG GGAAGCCCCG CCGGGTCCGG 
GTCCGAGAGG AACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P3h3 ( XM_030255169.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030111029.1 Mus musculus prolyl 3-hydroxylase 3 (P3h3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu89129
Clone ID Related Accession (Same CDS sequence)	XM_030255169.1
Accession Version	XM_030255169.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	prolyl 3-hydroxylase 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCGGC TCCTGCGGTT GTTGCTTTTG CTGCTGCTGC CTCCCCCGGG GTCCCCCGAG 
CCTCCCGAGC CCCCGGGCCT GGCTCAGTTG TCACCGGGGT CGCCTCCCCA GGCCCCCGAC 
TTGCTCTACG CGGACGGGCT GCGAGCCTAC TCGGCCGGGG CTTGGGCGCC CGCAGTGGCG 
CTGCTGCGAG AGGCGCTGCG GAGCCGGGCG GCGCTGGGCC GCGCGCGCCA GGAGTGCGGG 
GCGAGCTGCG CGGCCGAGCC GGGCGCCGCG CTCCCCTCCC AGCTCCTCGG AGCCCCACAT 
CCTGTCTCCG GGCCCGGGGT CTGGGAACCG CTGCTCCTGC GCGCCACGCT CCGCCGCGCC 
GAGTGCCTGA CCCAGTGCGC GGTGCGGAGG CTGGGCCCGG GGGGCGCGGC GCGGCTCCGC 
GTGGGGAGCG CGCTGCGGGA TGCCTTCCGC CGGCGGGAGC CTTACAACTA CCTGCAGAGG 
GCCTACTACC AGTTGAAGAA GCTGGACCTG GCAGCTTCTG CAGCACACAC CTTCTTTGTG 
GCAAACCCAA CACACCTGCA GATGCGGGAA GACATGGCTA AGTACAGAAG GATGTCTGCA 
ATCCGACCCC AGAGCTTCCG GGACCTGGTG ACGCCCCTAT ACTGGGCAGC TTATGACACT 
GGCCTGGAGC TTCTGGAGCA ACGGGAGGCA GCACTGGCTC TACCCCAACT AGAGGAGGCC 
CTGCAGGGGA GCCTGGCCCA CATGGAGAGC TGCCGTGCTG CCTGTGAGGG GCCTGAGGAG 
CACCAAGGGG CTGAAGAAGA GGGAGAAGGG AGCCAGGGAG GCCTGTATGA AGCCATTGCA 
GGACACTGGA TACGGGTTCT GCAGTGCAGG CAGCACTGTG TGGCAGACAC GGCCACCCGT 
CCTGGTCGCA GCTTCCCTGT CCAGGACTTC CTCCTCAGCC AGCTGAGACG GCTGCACGAG 
GCGTATGCTC AGGTGGGGAA CATGTCTCAG GCCATGGAAA ATGTCCTGAG TGTCCTGCTC 
TTCTACCCAG AGGATGAGGC TGCCAAAAAG GCTCTGAACC AGTACCAAAC TCAGTTGGGA 
GAGCCAAGAC CTGACCTGGG ACCACGAGAG GACATCCAGC GGTTCATCCT TCGCTCCCTC 
GGAGAGAAGA GACAGTTATA TTACGCCATG GAGCATCTGG GCACTAGCTT CAAGGACCCG 
GATTCTTGGA CCCCGGAGGC TCTCATCCCT AAGGCACTGA GAGAGAGGCT CAGAGAGGAT 
CAAGAGAAGA AGCCCTGGGA CCATCAGCCT CCACAGCAGA AGCCCTTGGC TCACTGGAAG 
GATGCCCTTC TGATGGAGGG TGTGACCCTC ACGCAGGACG CCCAGCAGCT GAATGGGTCG 
GAGCGAGCTG TCTTAGATGG GCTGCTAACT TCAGCCGAGT GTGGGGTTCT GCTGCAGCTG 
GCCAAGGATG CAGCACAGGC TGGAGCCAGG TCAGGCTACC GTGGCCGCCG CTCTCCTCAT 
AGTCCTCATG AACGCTTTGA GGGGCTCACG GTGCTCAAGG CTGCTCAGCT GGCCCGGGCA 
GGGACTGTGG GCAGGCCAGG CGCTAAGCTG CTTCTGGAGG TAAGTGAGCG AGTGCGGACT 
TTGACCCAGG CCTACTTCTC CCCGGAACGG CCACTGCATC TTTCCTTCAC CCACCTGGTG 
TGCCGAAGTG CCATAGAAGG TAACCACCAG AGAACCCCCC TCGCGCCACC CATCTTCACT 
GCAGAAAGTC CCCCGTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030111029.1
CDS	60..1820
Translation	MLRLLRLLLLLLLPPPGSPEPPEPPGLAQLSPGSPPQAPDLLYADGLRAYSAGAWAPAVALLREALRSRAALGRARQECGASCAAEPGAALPSQLLGAPHPVSGPGVWEPLLLRATLRRAECLTQCAVRRLGPGGAARLRVGSALRDAFRRREPYNYLQRAYYQLKKLDLAASAAHTFFVANPTHLQMREDMAKYRRMSAIRPQSFRDLVTPLYWAAYDTGLELLEQREAALALPQLEEALQGSLAHMESCRAACEGPEEHQGAEEEGEGSQGGLYEAIAGHWIRVLQCRQHCVADTATRPGRSFPVQDFLLSQLRRLHEAYAQVGNMSQAMENVLSVLLFYPEDEAAKKALNQYQTQLGEPRPDLGPREDIQRFILRSLGEKRQLYYAMEHLGTSFKDPDSWTPEALIPKALRERLREDQEKKPWDHQPPQQKPLAHWKDALLMEGVTLTQDAQQLNGSERAVLDGLLTSAECGVLLQLAKDAAQAGARSGYRGRRSPHSPHERFEGLTVLKAAQLARAGTVGRPGAKLLLEVSERVRTLTQAYFSPERPLHLSFTHLVCRSAIEGNHQRTPLAPPIFTAESPPW

Target ORF information:

RefSeq Version	XM_030255169.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus prolyl 3-hydroxylase 3 (P3h3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030255169.1

ORF Insert Sequence:

ATGCTCCGGC TCCTGCGGTT GTTGCTTTTG CTGCTGCTGC CTCCCCCGGG GTCCCCCGAG 
CCTCCCGAGC CCCCGGGCCT GGCTCAGTTG TCACCGGGGT CGCCTCCCCA GGCCCCCGAC 
TTGCTCTACG CGGACGGGCT GCGAGCCTAC TCGGCCGGGG CTTGGGCGCC CGCAGTGGCG 
CTGCTGCGAG AGGCGCTGCG GAGCCGGGCG GCGCTGGGCC GCGCGCGCCA GGAGTGCGGG 
GCGAGCTGCG CGGCCGAGCC GGGCGCCGCG CTCCCCTCCC AGCTCCTCGG AGCCCCACAT 
CCTGTCTCCG GGCCCGGGGT CTGGGAACCG CTGCTCCTGC GCGCCACGCT CCGCCGCGCC 
GAGTGCCTGA CCCAGTGCGC GGTGCGGAGG CTGGGCCCGG GGGGCGCGGC GCGGCTCCGC 
GTGGGGAGCG CGCTGCGGGA TGCCTTCCGC CGGCGGGAGC CTTACAACTA CCTGCAGAGG 
GCCTACTACC AGTTGAAGAA GCTGGACCTG GCAGCTTCTG CAGCACACAC CTTCTTTGTG 
GCAAACCCAA CACACCTGCA GATGCGGGAA GACATGGCTA AGTACAGAAG GATGTCTGCA 
ATCCGACCCC AGAGCTTCCG GGACCTGGTG ACGCCCCTAT ACTGGGCAGC TTATGACACT 
GGCCTGGAGC TTCTGGAGCA ACGGGAGGCA GCACTGGCTC TACCCCAACT AGAGGAGGCC 
CTGCAGGGGA GCCTGGCCCA CATGGAGAGC TGCCGTGCTG CCTGTGAGGG GCCTGAGGAG 
CACCAAGGGG CTGAAGAAGA GGGAGAAGGG AGCCAGGGAG GCCTGTATGA AGCCATTGCA 
GGACACTGGA TACGGGTTCT GCAGTGCAGG CAGCACTGTG TGGCAGACAC GGCCACCCGT 
CCTGGTCGCA GCTTCCCTGT CCAGGACTTC CTCCTCAGCC AGCTGAGACG GCTGCACGAG 
GCGTATGCTC AGGTGGGGAA CATGTCTCAG GCCATGGAAA ATGTCCTGAG TGTCCTGCTC 
TTCTACCCAG AGGATGAGGC TGCCAAAAAG GCTCTGAACC AGTACCAAAC TCAGTTGGGA 
GAGCCAAGAC CTGACCTGGG ACCACGAGAG GACATCCAGC GGTTCATCCT TCGCTCCCTC 
GGAGAGAAGA GACAGTTATA TTACGCCATG GAGCATCTGG GCACTAGCTT CAAGGACCCG 
GATTCTTGGA CCCCGGAGGC TCTCATCCCT AAGGCACTGA GAGAGAGGCT CAGAGAGGAT 
CAAGAGAAGA AGCCCTGGGA CCATCAGCCT CCACAGCAGA AGCCCTTGGC TCACTGGAAG 
GATGCCCTTC TGATGGAGGG TGTGACCCTC ACGCAGGACG CCCAGCAGCT GAATGGGTCG 
GAGCGAGCTG TCTTAGATGG GCTGCTAACT TCAGCCGAGT GTGGGGTTCT GCTGCAGCTG 
GCCAAGGATG CAGCACAGGC TGGAGCCAGG TCAGGCTACC GTGGCCGCCG CTCTCCTCAT 
AGTCCTCATG AACGCTTTGA GGGGCTCACG GTGCTCAAGG CTGCTCAGCT GGCCCGGGCA 
GGGACTGTGG GCAGGCCAGG CGCTAAGCTG CTTCTGGAGG TAAGTGAGCG AGTGCGGACT 
TTGACCCAGG CCTACTTCTC CCCGGAACGG CCACTGCATC TTTCCTTCAC CCACCTGGTG 
TGCCGAAGTG CCATAGAAGG TAACCACCAG AGAACCCCCC TCGCGCCACC CATCTTCACT 
GCAGAAAGTC CCCCGTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P3h3 ( NM_013534.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038562.2 Mus musculus prolyl 3-hydroxylase 3 (P3h3), mRNA.

Price & Availability↑

CloneID	OMu09556
Clone ID Related Accession (Same CDS sequence)	NM_013534.4 , NM_013534.5
Accession Version	NM_013534.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-27
Organism	Mus musculus(house mouse)
Product	prolyl 3-hydroxylase 3 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC142254.4. On Nov 17, 2006 this sequence version replaced NM_013534.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AJ441086.1, BC003726.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGCTCCGGC TCCTGCGGTT GTTGCTTTTG CTGCTGCTGC CTCCCCCGGG GTCCCCCGAG 
CCTCCCGAGC CCCCGGGCCT GGCTCAGTTG TCACCGGGGT CGCCTCCCCA GGCCCCCGAC 
TTGCTCTACG CGGACGGGCT GCGAGCCTAC TCGGCCGGGG CTTGGGCGCC CGCAGTGGCG 
CTGCTGCGAG AGGCGCTGCG GAGCCGGGCG GCGCTGGGCC GCGCGCGCCA GGAGTGCGGG 
GCGAGCTGCG CGGCCGAGCC GGGCGCCGCG CTCCCCTCCC AGCTCCTCGG AGCCCCACAT 
CCTGTCTCCG GGCCCGGGGT CTGGGAACCG CTGCTCCTGC GCGCCACGCT CCGCCGCGCC 
GAGTGCCTGA CCCAGTGCGC GGTGCGGAGG CTGGGCCCGG GGGGCGCGGC GCGGCTCCGC 
GTGGGGAGCG CGCTGCGGGA TGCCTTCCGC CGGCGGGAGC CTTACAACTA CCTGCAGAGG 
GCCTACTACC AGTTGAAGAA GCTGGACCTG GCAGCTTCTG CAGCACACAC CTTCTTTGTG 
GCAAACCCAA CACACCTGCA GATGCGGGAA GACATGGCTA AGTACAGAAG GATGTCTGCA 
ATCCGACCCC AGAGCTTCCG GGACCTGGTG ACGCCCCTAT ACTGGGCAGC TTATGACACT 
GGCCTGGAGC TTCTGGAGCA ACGGGAGGCA GCACTGGCTC TACCCCAACT AGAGGAGGCC 
CTGCAGGGGA GCCTGGCCCA CATGGAGAGC TGCCGTGCTG CCTGTGAGGG GCCTGAGGAG 
CACCAAGGGG CTGAAGAAGA GGGAGAAGGG AGCCAGGGAG GCCTGTATGA AGCCATTGCA 
GGACACTGGA TACGGGTTCT GCAGTGCAGG CAGCACTGTG TGGCAGACAC GGCCACCCGT 
CCTGGTCGCA GCTTCCCTGT CCAGGACTTC CTCCTCAGCC AGCTGAGACG GCTGCACGAG 
GCGTATGCTC AGGTGGGGAA CATGTCTCAG GCCATGGAAA ATGTCCTGAG TGTCCTGCTC 
TTCTACCCAG AGGATGAGGC TGCCAAAAAG GCTCTGAACC AGTACCAAAC TCAGTTGGGA 
GAGCCAAGAC CTGACCTGGG ACCACGAGAG GACATCCAGC GGTTCATCCT TCGCTCCCTC 
GGAGAGAAGA GACAGTTATA TTACGCCATG GAGCATCTGG GCACTAGCTT CAAGGACCCG 
GATTCTTGGA CCCCGGAGGC TCTCATCCCT AAGGCACTGA GAGAGAGGCT CAGAGAGGAT 
CAAGAGAAGA AGCCCTGGGA CCATCAGCCT CCACAGCAGA AGCCCTTGGC TCACTGGAAG 
GATGCCCTTC TGATGGAGGG TGTGACCCTC ACGCAGGACG CCCAGCAGCT GAATGGGTCG 
GAGCGAGCTG TCTTAGATGG GCTGCTAACT TCAGCCGAGT GTGGGGTTCT GCTGCAGCTG 
GCCAAGGATG CAGCACAGGC TGGAGCCAGG TCAGGCTACC GTGGCCGCCG CTCTCCTCAT 
AGTCCTCATG AACGCTTTGA GGGGCTCACG GTGCTCAAGG CTGCTCAGCT GGCCCGGGCA 
GGGACTGTGG GCAGGCCAGG CGCTAAGCTG CTTCTGGAGG TAAGTGAGCG AGTGCGGACT 
TTGACCCAGG CCTACTTCTC CCCGGAACGG CCACTGCATC TTTCCTTCAC CCACCTGGTG 
TGCCGAAGTG CCATAGAAGG TGAACAAGAG CAGCGTATGG ACCTGAGTCA CCCGGTTCAT 
GCAGACAACT GTGTCCTGGA CCCCGACACT GGCGAATGCT GGCGGGAGCC CCCAGCCTAC 
ACCTATCGAG ACTATAGTGG ACTCCTCTAC CTCAATGATG ACTTCAAGGG AGGCGACCTG 
TTCTTCACGC AGCCCAACGC CCTCACTGTC ACGGCTCAGG TCCGTCCTCG GTGTGGGCGC 
CTTGTGGCCT TCAGCTCTGG TGGTGAGAAT CCCCATGGTG TATGGGCTGT GACTCGGGGA 
CGGCGCTGTG CCCTAGCACT GTGGCACACG TGGGCACCTG AGCACAGTGA ACAGGAGTGG 
ACAGAAGCCA AAGAGCTGCT GCAGGAGGAA GAGGAGGAAG AAGAGGAGGA AGACATTCTC 
AGCAGAGACC CTTCCCCAGA ACCCCCAAGT CACAAGCTTC AGCGAGTCCA GGAGAAAGCT 
GGGAAGCCCC GCCGGGTCCG GGTCCGAGAG GAACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038562.2
CDS	1..2199
Translation	MLRLLRLLLLLLLPPPGSPEPPEPPGLAQLSPGSPPQAPDLLYADGLRAYSAGAWAPAVALLREALRSRAALGRARQECGASCAAEPGAALPSQLLGAPHPVSGPGVWEPLLLRATLRRAECLTQCAVRRLGPGGAARLRVGSALRDAFRRREPYNYLQRAYYQLKKLDLAASAAHTFFVANPTHLQMREDMAKYRRMSAIRPQSFRDLVTPLYWAAYDTGLELLEQREAALALPQLEEALQGSLAHMESCRAACEGPEEHQGAEEEGEGSQGGLYEAIAGHWIRVLQCRQHCVADTATRPGRSFPVQDFLLSQLRRLHEAYAQVGNMSQAMENVLSVLLFYPEDEAAKKALNQYQTQLGEPRPDLGPREDIQRFILRSLGEKRQLYYAMEHLGTSFKDPDSWTPEALIPKALRERLREDQEKKPWDHQPPQQKPLAHWKDALLMEGVTLTQDAQQLNGSERAVLDGLLTSAECGVLLQLAKDAAQAGARSGYRGRRSPHSPHERFEGLTVLKAAQLARAGTVGRPGAKLLLEVSERVRTLTQAYFSPERPLHLSFTHLVCRSAIEGEQEQRMDLSHPVHADNCVLDPDTGECWREPPAYTYRDYSGLLYLNDDFKGGDLFFTQPNALTVTAQVRPRCGRLVAFSSGGENPHGVWAVTRGRRCALALWHTWAPEHSEQEWTEAKELLQEEEEEEEEEDILSRDPSPEPPSHKLQRVQEKAGKPRRVRVREEL

Target ORF information:

RefSeq Version	NM_013534.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus prolyl 3-hydroxylase 3 (P3h3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013534.4

ORF Insert Sequence:

ATGCTCCGGC TCCTGCGGTT GTTGCTTTTG CTGCTGCTGC CTCCCCCGGG GTCCCCCGAG 
CCTCCCGAGC CCCCGGGCCT GGCTCAGTTG TCACCGGGGT CGCCTCCCCA GGCCCCCGAC 
TTGCTCTACG CGGACGGGCT GCGAGCCTAC TCGGCCGGGG CTTGGGCGCC CGCAGTGGCG 
CTGCTGCGAG AGGCGCTGCG GAGCCGGGCG GCGCTGGGCC GCGCGCGCCA GGAGTGCGGG 
GCGAGCTGCG CGGCCGAGCC GGGCGCCGCG CTCCCCTCCC AGCTCCTCGG AGCCCCACAT 
CCTGTCTCCG GGCCCGGGGT CTGGGAACCG CTGCTCCTGC GCGCCACGCT CCGCCGCGCC 
GAGTGCCTGA CCCAGTGCGC GGTGCGGAGG CTGGGCCCGG GGGGCGCGGC GCGGCTCCGC 
GTGGGGAGCG CGCTGCGGGA TGCCTTCCGC CGGCGGGAGC CTTACAACTA CCTGCAGAGG 
GCCTACTACC AGTTGAAGAA GCTGGACCTG GCAGCTTCTG CAGCACACAC CTTCTTTGTG 
GCAAACCCAA CACACCTGCA GATGCGGGAA GACATGGCTA AGTACAGAAG GATGTCTGCA 
ATCCGACCCC AGAGCTTCCG GGACCTGGTG ACGCCCCTAT ACTGGGCAGC TTATGACACT 
GGCCTGGAGC TTCTGGAGCA ACGGGAGGCA GCACTGGCTC TACCCCAACT AGAGGAGGCC 
CTGCAGGGGA GCCTGGCCCA CATGGAGAGC TGCCGTGCTG CCTGTGAGGG GCCTGAGGAG 
CACCAAGGGG CTGAAGAAGA GGGAGAAGGG AGCCAGGGAG GCCTGTATGA AGCCATTGCA 
GGACACTGGA TACGGGTTCT GCAGTGCAGG CAGCACTGTG TGGCAGACAC GGCCACCCGT 
CCTGGTCGCA GCTTCCCTGT CCAGGACTTC CTCCTCAGCC AGCTGAGACG GCTGCACGAG 
GCGTATGCTC AGGTGGGGAA CATGTCTCAG GCCATGGAAA ATGTCCTGAG TGTCCTGCTC 
TTCTACCCAG AGGATGAGGC TGCCAAAAAG GCTCTGAACC AGTACCAAAC TCAGTTGGGA 
GAGCCAAGAC CTGACCTGGG ACCACGAGAG GACATCCAGC GGTTCATCCT TCGCTCCCTC 
GGAGAGAAGA GACAGTTATA TTACGCCATG GAGCATCTGG GCACTAGCTT CAAGGACCCG 
GATTCTTGGA CCCCGGAGGC TCTCATCCCT AAGGCACTGA GAGAGAGGCT CAGAGAGGAT 
CAAGAGAAGA AGCCCTGGGA CCATCAGCCT CCACAGCAGA AGCCCTTGGC TCACTGGAAG 
GATGCCCTTC TGATGGAGGG TGTGACCCTC ACGCAGGACG CCCAGCAGCT GAATGGGTCG 
GAGCGAGCTG TCTTAGATGG GCTGCTAACT TCAGCCGAGT GTGGGGTTCT GCTGCAGCTG 
GCCAAGGATG CAGCACAGGC TGGAGCCAGG TCAGGCTACC GTGGCCGCCG CTCTCCTCAT 
AGTCCTCATG AACGCTTTGA GGGGCTCACG GTGCTCAAGG CTGCTCAGCT GGCCCGGGCA 
GGGACTGTGG GCAGGCCAGG CGCTAAGCTG CTTCTGGAGG TAAGTGAGCG AGTGCGGACT 
TTGACCCAGG CCTACTTCTC CCCGGAACGG CCACTGCATC TTTCCTTCAC CCACCTGGTG 
TGCCGAAGTG CCATAGAAGG TGAACAAGAG CAGCGTATGG ACCTGAGTCA CCCGGTTCAT 
GCAGACAACT GTGTCCTGGA CCCCGACACT GGCGAATGCT GGCGGGAGCC CCCAGCCTAC 
ACCTATCGAG ACTATAGTGG ACTCCTCTAC CTCAATGATG ACTTCAAGGG AGGCGACCTG 
TTCTTCACGC AGCCCAACGC CCTCACTGTC ACGGCTCAGG TCCGTCCTCG GTGTGGGCGC 
CTTGTGGCCT TCAGCTCTGG TGGTGAGAAT CCCCATGGTG TATGGGCTGT GACTCGGGGA 
CGGCGCTGTG CCCTAGCACT GTGGCACACG TGGGCACCTG AGCACAGTGA ACAGGAGTGG 
ACAGAAGCCA AAGAGCTGCT GCAGGAGGAA GAGGAGGAAG AAGAGGAGGA AGACATTCTC 
AGCAGAGACC CTTCCCCAGA ACCCCCAAGT CACAAGCTTC AGCGAGTCCA GGAGAAAGCT 
GGGAAGCCCC GCCGGGTCCG GGTCCGAGAG GAACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P3h3 ( NM_013534.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038562.2 Mus musculus prolyl 3-hydroxylase 3 (P3h3), transcript variant 1, mRNA.

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CloneID	OMu09556
Clone ID Related Accession (Same CDS sequence)	NM_013534.4 , NM_013534.5
Accession Version	NM_013534.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	prolyl 3-hydroxylase 3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC142254.4. On May 29, 2018 this sequence version replaced NM_013534.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AJ441086.1, BC003726.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTCCGGC TCCTGCGGTT GTTGCTTTTG CTGCTGCTGC CTCCCCCGGG GTCCCCCGAG 
CCTCCCGAGC CCCCGGGCCT GGCTCAGTTG TCACCGGGGT CGCCTCCCCA GGCCCCCGAC 
TTGCTCTACG CGGACGGGCT GCGAGCCTAC TCGGCCGGGG CTTGGGCGCC CGCAGTGGCG 
CTGCTGCGAG AGGCGCTGCG GAGCCGGGCG GCGCTGGGCC GCGCGCGCCA GGAGTGCGGG 
GCGAGCTGCG CGGCCGAGCC GGGCGCCGCG CTCCCCTCCC AGCTCCTCGG AGCCCCACAT 
CCTGTCTCCG GGCCCGGGGT CTGGGAACCG CTGCTCCTGC GCGCCACGCT CCGCCGCGCC 
GAGTGCCTGA CCCAGTGCGC GGTGCGGAGG CTGGGCCCGG GGGGCGCGGC GCGGCTCCGC 
GTGGGGAGCG CGCTGCGGGA TGCCTTCCGC CGGCGGGAGC CTTACAACTA CCTGCAGAGG 
GCCTACTACC AGTTGAAGAA GCTGGACCTG GCAGCTTCTG CAGCACACAC CTTCTTTGTG 
GCAAACCCAA CACACCTGCA GATGCGGGAA GACATGGCTA AGTACAGAAG GATGTCTGCA 
ATCCGACCCC AGAGCTTCCG GGACCTGGTG ACGCCCCTAT ACTGGGCAGC TTATGACACT 
GGCCTGGAGC TTCTGGAGCA ACGGGAGGCA GCACTGGCTC TACCCCAACT AGAGGAGGCC 
CTGCAGGGGA GCCTGGCCCA CATGGAGAGC TGCCGTGCTG CCTGTGAGGG GCCTGAGGAG 
CACCAAGGGG CTGAAGAAGA GGGAGAAGGG AGCCAGGGAG GCCTGTATGA AGCCATTGCA 
GGACACTGGA TACGGGTTCT GCAGTGCAGG CAGCACTGTG TGGCAGACAC GGCCACCCGT 
CCTGGTCGCA GCTTCCCTGT CCAGGACTTC CTCCTCAGCC AGCTGAGACG GCTGCACGAG 
GCGTATGCTC AGGTGGGGAA CATGTCTCAG GCCATGGAAA ATGTCCTGAG TGTCCTGCTC 
TTCTACCCAG AGGATGAGGC TGCCAAAAAG GCTCTGAACC AGTACCAAAC TCAGTTGGGA 
GAGCCAAGAC CTGACCTGGG ACCACGAGAG GACATCCAGC GGTTCATCCT TCGCTCCCTC 
GGAGAGAAGA GACAGTTATA TTACGCCATG GAGCATCTGG GCACTAGCTT CAAGGACCCG 
GATTCTTGGA CCCCGGAGGC TCTCATCCCT AAGGCACTGA GAGAGAGGCT CAGAGAGGAT 
CAAGAGAAGA AGCCCTGGGA CCATCAGCCT CCACAGCAGA AGCCCTTGGC TCACTGGAAG 
GATGCCCTTC TGATGGAGGG TGTGACCCTC ACGCAGGACG CCCAGCAGCT GAATGGGTCG 
GAGCGAGCTG TCTTAGATGG GCTGCTAACT TCAGCCGAGT GTGGGGTTCT GCTGCAGCTG 
GCCAAGGATG CAGCACAGGC TGGAGCCAGG TCAGGCTACC GTGGCCGCCG CTCTCCTCAT 
AGTCCTCATG AACGCTTTGA GGGGCTCACG GTGCTCAAGG CTGCTCAGCT GGCCCGGGCA 
GGGACTGTGG GCAGGCCAGG CGCTAAGCTG CTTCTGGAGG TAAGTGAGCG AGTGCGGACT 
TTGACCCAGG CCTACTTCTC CCCGGAACGG CCACTGCATC TTTCCTTCAC CCACCTGGTG 
TGCCGAAGTG CCATAGAAGG TGAACAAGAG CAGCGTATGG ACCTGAGTCA CCCGGTTCAT 
GCAGACAACT GTGTCCTGGA CCCCGACACT GGCGAATGCT GGCGGGAGCC CCCAGCCTAC 
ACCTATCGAG ACTATAGTGG ACTCCTCTAC CTCAATGATG ACTTCAAGGG AGGCGACCTG 
TTCTTCACGC AGCCCAACGC CCTCACTGTC ACGGCTCAGG TCCGTCCTCG GTGTGGGCGC 
CTTGTGGCCT TCAGCTCTGG TGGTGAGAAT CCCCATGGTG TATGGGCTGT GACTCGGGGA 
CGGCGCTGTG CCCTAGCACT GTGGCACACG TGGGCACCTG AGCACAGTGA ACAGGAGTGG 
ACAGAAGCCA AAGAGCTGCT GCAGGAGGAA GAGGAGGAAG AAGAGGAGGA AGACATTCTC 
AGCAGAGACC CTTCCCCAGA ACCCCCAAGT CACAAGCTTC AGCGAGTCCA GGAGAAAGCT 
GGGAAGCCCC GCCGGGTCCG GGTCCGAGAG GAACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038562.2
CDS	31..2229
Translation	MLRLLRLLLLLLLPPPGSPEPPEPPGLAQLSPGSPPQAPDLLYADGLRAYSAGAWAPAVALLREALRSRAALGRARQECGASCAAEPGAALPSQLLGAPHPVSGPGVWEPLLLRATLRRAECLTQCAVRRLGPGGAARLRVGSALRDAFRRREPYNYLQRAYYQLKKLDLAASAAHTFFVANPTHLQMREDMAKYRRMSAIRPQSFRDLVTPLYWAAYDTGLELLEQREAALALPQLEEALQGSLAHMESCRAACEGPEEHQGAEEEGEGSQGGLYEAIAGHWIRVLQCRQHCVADTATRPGRSFPVQDFLLSQLRRLHEAYAQVGNMSQAMENVLSVLLFYPEDEAAKKALNQYQTQLGEPRPDLGPREDIQRFILRSLGEKRQLYYAMEHLGTSFKDPDSWTPEALIPKALRERLREDQEKKPWDHQPPQQKPLAHWKDALLMEGVTLTQDAQQLNGSERAVLDGLLTSAECGVLLQLAKDAAQAGARSGYRGRRSPHSPHERFEGLTVLKAAQLARAGTVGRPGAKLLLEVSERVRTLTQAYFSPERPLHLSFTHLVCRSAIEGEQEQRMDLSHPVHADNCVLDPDTGECWREPPAYTYRDYSGLLYLNDDFKGGDLFFTQPNALTVTAQVRPRCGRLVAFSSGGENPHGVWAVTRGRRCALALWHTWAPEHSEQEWTEAKELLQEEEEEEEEEDILSRDPSPEPPSHKLQRVQEKAGKPRRVRVREEL

Target ORF information:

RefSeq Version	NM_013534.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus prolyl 3-hydroxylase 3 (P3h3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013534.5

ORF Insert Sequence:

ATGCTCCGGC TCCTGCGGTT GTTGCTTTTG CTGCTGCTGC CTCCCCCGGG GTCCCCCGAG 
CCTCCCGAGC CCCCGGGCCT GGCTCAGTTG TCACCGGGGT CGCCTCCCCA GGCCCCCGAC 
TTGCTCTACG CGGACGGGCT GCGAGCCTAC TCGGCCGGGG CTTGGGCGCC CGCAGTGGCG 
CTGCTGCGAG AGGCGCTGCG GAGCCGGGCG GCGCTGGGCC GCGCGCGCCA GGAGTGCGGG 
GCGAGCTGCG CGGCCGAGCC GGGCGCCGCG CTCCCCTCCC AGCTCCTCGG AGCCCCACAT 
CCTGTCTCCG GGCCCGGGGT CTGGGAACCG CTGCTCCTGC GCGCCACGCT CCGCCGCGCC 
GAGTGCCTGA CCCAGTGCGC GGTGCGGAGG CTGGGCCCGG GGGGCGCGGC GCGGCTCCGC 
GTGGGGAGCG CGCTGCGGGA TGCCTTCCGC CGGCGGGAGC CTTACAACTA CCTGCAGAGG 
GCCTACTACC AGTTGAAGAA GCTGGACCTG GCAGCTTCTG CAGCACACAC CTTCTTTGTG 
GCAAACCCAA CACACCTGCA GATGCGGGAA GACATGGCTA AGTACAGAAG GATGTCTGCA 
ATCCGACCCC AGAGCTTCCG GGACCTGGTG ACGCCCCTAT ACTGGGCAGC TTATGACACT 
GGCCTGGAGC TTCTGGAGCA ACGGGAGGCA GCACTGGCTC TACCCCAACT AGAGGAGGCC 
CTGCAGGGGA GCCTGGCCCA CATGGAGAGC TGCCGTGCTG CCTGTGAGGG GCCTGAGGAG 
CACCAAGGGG CTGAAGAAGA GGGAGAAGGG AGCCAGGGAG GCCTGTATGA AGCCATTGCA 
GGACACTGGA TACGGGTTCT GCAGTGCAGG CAGCACTGTG TGGCAGACAC GGCCACCCGT 
CCTGGTCGCA GCTTCCCTGT CCAGGACTTC CTCCTCAGCC AGCTGAGACG GCTGCACGAG 
GCGTATGCTC AGGTGGGGAA CATGTCTCAG GCCATGGAAA ATGTCCTGAG TGTCCTGCTC 
TTCTACCCAG AGGATGAGGC TGCCAAAAAG GCTCTGAACC AGTACCAAAC TCAGTTGGGA 
GAGCCAAGAC CTGACCTGGG ACCACGAGAG GACATCCAGC GGTTCATCCT TCGCTCCCTC 
GGAGAGAAGA GACAGTTATA TTACGCCATG GAGCATCTGG GCACTAGCTT CAAGGACCCG 
GATTCTTGGA CCCCGGAGGC TCTCATCCCT AAGGCACTGA GAGAGAGGCT CAGAGAGGAT 
CAAGAGAAGA AGCCCTGGGA CCATCAGCCT CCACAGCAGA AGCCCTTGGC TCACTGGAAG 
GATGCCCTTC TGATGGAGGG TGTGACCCTC ACGCAGGACG CCCAGCAGCT GAATGGGTCG 
GAGCGAGCTG TCTTAGATGG GCTGCTAACT TCAGCCGAGT GTGGGGTTCT GCTGCAGCTG 
GCCAAGGATG CAGCACAGGC TGGAGCCAGG TCAGGCTACC GTGGCCGCCG CTCTCCTCAT 
AGTCCTCATG AACGCTTTGA GGGGCTCACG GTGCTCAAGG CTGCTCAGCT GGCCCGGGCA 
GGGACTGTGG GCAGGCCAGG CGCTAAGCTG CTTCTGGAGG TAAGTGAGCG AGTGCGGACT 
TTGACCCAGG CCTACTTCTC CCCGGAACGG CCACTGCATC TTTCCTTCAC CCACCTGGTG 
TGCCGAAGTG CCATAGAAGG TGAACAAGAG CAGCGTATGG ACCTGAGTCA CCCGGTTCAT 
GCAGACAACT GTGTCCTGGA CCCCGACACT GGCGAATGCT GGCGGGAGCC CCCAGCCTAC 
ACCTATCGAG ACTATAGTGG ACTCCTCTAC CTCAATGATG ACTTCAAGGG AGGCGACCTG 
TTCTTCACGC AGCCCAACGC CCTCACTGTC ACGGCTCAGG TCCGTCCTCG GTGTGGGCGC 
CTTGTGGCCT TCAGCTCTGG TGGTGAGAAT CCCCATGGTG TATGGGCTGT GACTCGGGGA 
CGGCGCTGTG CCCTAGCACT GTGGCACACG TGGGCACCTG AGCACAGTGA ACAGGAGTGG 
ACAGAAGCCA AAGAGCTGCT GCAGGAGGAA GAGGAGGAAG AAGAGGAGGA AGACATTCTC 
AGCAGAGACC CTTCCCCAGA ACCCCCAAGT CACAAGCTTC AGCGAGTCCA GGAGAAAGCT 
GGGAAGCCCC GCCGGGTCCG GGTCCGAGAG GAACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cellular heterogeneity in the ureteric progenitor niche and distinct profiles of branching morphogenesis in organ development.
Development (Cambridge, England)144(17)3177-3188(2017 09)
Rutledge EA,Benazet JD,McMahon AP

P3h3-null and Sc65-null Mice Phenocopy the Collagen Lysine Under-hydroxylation and Cross-linking Abnormality of Ehlers-Danlos Syndrome Type VIA.
The Journal of biological chemistry292(9)3877-3887(2017 03)
Hudson DM,Weis M,Rai J,Joeng KS,Dimori M,Lee BH,Morello R,Eyre DR

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Sc65-Null Mice Provide Evidence for a Novel Endoplasmic Reticulum Complex Regulating Collagen Lysyl Hydroxylation.
PLoS genetics12(4)e1006002(2016 Apr)
Heard ME,Besio R,Weis M,Rai J,Hudson DM,Dimori M,Zimmerman SM,Kamykowski JA,Hogue WR,Swain FL,Burdine MS,Mackintosh SG,Tackett AJ,Suva LJ,Eyre DR,Morello R

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

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P3h3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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