Gpr65 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gpr65
Entrez Gene ID	14744
Full Name	G-protein coupled receptor 65
Synonyms	Dig1,Gpcr25,TDAG8
General protein information	Preferred Names G-protein coupled receptor 65 Names psychosine receptor G-protein coupled receptor 25 T-cell death-associated gene 8 protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 E

mRNA and Protein(s)

mRNA	Protein	Name
NM_008152.3	NP_032178.1	psychosine receptor

Pathways from BioSystems

Homologies

Homo sapiens (human)	GPR65	NP_003599.2
Canis lupus familiaris (dog)	GPR65	XP_005623778.1
Rattus norvegicus (Norway rat)	Gpr65	NP_001100221.1
Gallus gallus (chicken)	GPR65	XP_004941908.1
	GPR65	XP_001082670.1
Xenopus tropicalis (tropical clawed frog)	gpr65	XP_002933239.1
Bos taurus (cattle)	GPR65	NP_001094773.1
Pan troglodytes (chimpanzee)	GPR65	XP_523005.1
Mus musculus (house mouse)	Gpr65	NP_032178.1

Gene Ontology
Bibliography

Related articles in PubMed

TDAG8, TRPV1, and ASIC3 involved in establishing hyperalgesic priming in experimental rheumatoid arthritis.
Hsieh WS, Kung CC, Huang SL, Lin SC, Sun WH
Scientific reports7(1)8870(2017 Aug)

The proton-sensing G protein-coupled receptor T-cell death-associated gene 8 (TDAG8) shows cardioprotective effects against myocardial infarction.
Nagasaka A, Mogi C, Ono H, Nishi T, Horii Y, Ohba Y, Sato K, Nakaya M, Okajima F, Kurose H
Scientific reports7(1)7812(2017 Aug)

TDAG8 involved in initiating inflammatory hyperalgesia and establishing hyperalgesic priming in mice.
Dai SP, Huang YH, Chang CJ, Huang YF, Hsieh WS, Tabata Y, Ishii S, Sun WH
Scientific reports741415(2017 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

data identify TDAG8-dependent microglial acid sensing as a unique chemosensor for detecting and translating hypercapnia to fear-associated behavioral and physiological responses.
Title: Microglial Acid Sensing Regulates Carbon Dioxide-Evoked Fear.

TDAG8 is a negative regulator for lung neutrophilic inflammation and injury, in part, through the inhibition of chemokine production.
Title: Protective Role of Proton-Sensing TDAG8 in Lipopolysaccharide-Induced Acute Lung Injury.

TDAG8(-/-) mice showed significantly higher consumption of sucrose compared to wild type mice although sucrose preference was not significantly different between genotypes.
Title: A potential role for the acid-sensing T cell death associated gene-8 (TDAG8) receptor in depression-like behavior.

The results of this study indicated that the proton-sensing G protein-coupled receptor GPR65 may be involved in a mechanism that supports survival of photoreceptors in the degenerating retina.
Title: Increased expression of the proton-sensing G protein-coupled receptor Gpr65 during retinal degeneration.

This study demonstrates differential regulation of eosinophils and mast cells in inflammatory tissue, with mast cell viability and accumulation being independent of GPR65.
Title: Differential eosinophil and mast cell regulation: mast cell viability and accumulation in inflammatory tissue are independent of proton-sensing receptor GPR65.

The following Gpr65 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gpr65 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu15977	NM_008152.3 Latest version!	Mus musculus G-protein coupled receptor 65 (Gpr65), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gpr65 ( NM_008152.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032178.1 Mus musculus G-protein coupled receptor 65 (Gpr65), mRNA.

Price & Availability↑

CloneID	OMu15977
Clone ID Related Accession (Same CDS sequence)	NM_008152.3
Accession Version	NM_008152.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-03
Organism	Mus musculus(house mouse)
Product	psychosine receptor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK134113.1, AK142853.1 and BF453845.1. On Nov 3, 2012 this sequence version replaced NM_008152.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK134113.1, U39827.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGGCGATGA ACAGCATGTG CATTGAAGAG CAGCGCCACC TCGAACACTA TTTGTTCCCG 
GTGGTCTACA TAATTGTGTT TATAGTCAGC GTCCCAGCCA ACATCGGATC TTTATGCGTA 
TCCTTTCTGC AAGCGAAGAA GGAAAATGAG CTAGGGATTT ACCTCTTCAG TCTGTCCCTG 
TCAGACCTGC TGTATGCGCT GACTCTGCCC CTCTGGATCA ATTACACTTG GAATAAAGAC 
AACTGGACTT TCTCTCCCAC CTTGTGCAAA GGAAGCGTTT TCTTCACCTA CATGAACTTT 
TACAGCAGCA CGGCGTTCCT CACTTGCATT GCCCTGGACC GCTATTTAGC AGTCGTCTAC 
CCTCTGAAGT TTTCCTTCCT AAGAACGAGA AGATTCGCGT TTATTACCAG CCTCTCCATC 
TGGATATTAG AGTCCTTCTT TAACTCTATG CTTCTGTGGA AAGATGAAAC GAGTGTTGAA 
TATTGTGACT CGGACAAATC TAATTTCACT CTCTGCTATG ACAAATACCC TCTGGAGAAA 
TGGCAGATAA ACCTCAACCT GTTTCGGACG TGCATGGGCT ACGCAATACC CTTGATCACC 
ATCATGATCT GCAACCATAA AGTCTACCGA GCTGTGCGGC ACAACCAAGC CACGGAAAAC 
AGCGAGAAGA GAAGGATCAT AAAGTTGCTT GCTAGCATCA CGTTGACTTT CGTCCTATGC 
TTTACCCCCT TCCACGTGAT GGTGCTCATC CGCTGCGTTT TAGAGCGCGA CATGAACGTC 
AATGACAAGT CTGGATGGCA GACGTTTACG GTGTACAGAG TCACAGTAGC CCTGACGAGT 
CTAAACTGTG TTGCCGATCC CATTCTGTAC TGCTTTGTGA CTGAGACGGG GAGAGCTGAT 
ATGTGGAACA TATTAAAATT GTGTACTAGG AAACACAATA GACACCAAGG GAAAAAAAGG 
GACATACTTT CTGTGTCCAC AAGAGATGCT GTAGAATTAG AGATTATAGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032178.1
CDS	515..1528
Translation	MAMNSMCIEEQRHLEHYLFPVVYIIVFIVSVPANIGSLCVSFLQAKKENELGIYLFSLSLSDLLYALTLPLWINYTWNKDNWTFSPTLCKGSVFFTYMNFYSSTAFLTCIALDRYLAVVYPLKFSFLRTRRFAFITSLSIWILESFFNSMLLWKDETSVEYCDSDKSNFTLCYDKYPLEKWQINLNLFRTCMGYAIPLITIMICNHKVYRAVRHNQATENSEKRRIIKLLASITLTFVLCFTPFHVMVLIRCVLERDMNVNDKSGWQTFTVYRVTVALTSLNCVADPILYCFVTETGRADMWNILKLCTRKHNRHQGKKRDILSVSTRDAVELEIID

Target ORF information:

RefSeq Version	NM_008152.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus G-protein coupled receptor 65 (Gpr65), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008152.3

ORF Insert Sequence:

ATGGCGATGA ACAGCATGTG CATTGAAGAG CAGCGCCACC TCGAACACTA TTTGTTCCCG 
GTGGTCTACA TAATTGTGTT TATAGTCAGC GTCCCAGCCA ACATCGGATC TTTATGCGTA 
TCCTTTCTGC AAGCGAAGAA GGAAAATGAG CTAGGGATTT ACCTCTTCAG TCTGTCCCTG 
TCAGACCTGC TGTATGCGCT GACTCTGCCC CTCTGGATCA ATTACACTTG GAATAAAGAC 
AACTGGACTT TCTCTCCCAC CTTGTGCAAA GGAAGCGTTT TCTTCACCTA CATGAACTTT 
TACAGCAGCA CGGCGTTCCT CACTTGCATT GCCCTGGACC GCTATTTAGC AGTCGTCTAC 
CCTCTGAAGT TTTCCTTCCT AAGAACGAGA AGATTCGCGT TTATTACCAG CCTCTCCATC 
TGGATATTAG AGTCCTTCTT TAACTCTATG CTTCTGTGGA AAGATGAAAC GAGTGTTGAA 
TATTGTGACT CGGACAAATC TAATTTCACT CTCTGCTATG ACAAATACCC TCTGGAGAAA 
TGGCAGATAA ACCTCAACCT GTTTCGGACG TGCATGGGCT ACGCAATACC CTTGATCACC 
ATCATGATCT GCAACCATAA AGTCTACCGA GCTGTGCGGC ACAACCAAGC CACGGAAAAC 
AGCGAGAAGA GAAGGATCAT AAAGTTGCTT GCTAGCATCA CGTTGACTTT CGTCCTATGC 
TTTACCCCCT TCCACGTGAT GGTGCTCATC CGCTGCGTTT TAGAGCGCGA CATGAACGTC 
AATGACAAGT CTGGATGGCA GACGTTTACG GTGTACAGAG TCACAGTAGC CCTGACGAGT 
CTAAACTGTG TTGCCGATCC CATTCTGTAC TGCTTTGTGA CTGAGACGGG GAGAGCTGAT 
ATGTGGAACA TATTAAAATT GTGTACTAGG AAACACAATA GACACCAAGG GAAAAAAAGG 
GACATACTTT CTGTGTCCAC AAGAGATGCT GTAGAATTAG AGATTATAGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TDAG8, TRPV1, and ASIC3 involved in establishing hyperalgesic priming in experimental rheumatoid arthritis.
Scientific reports7(1)8870(2017 Aug)
Hsieh WS,Kung CC,Huang SL,Lin SC,Sun WH

The proton-sensing G protein-coupled receptor T-cell death-associated gene 8 (TDAG8) shows cardioprotective effects against myocardial infarction.
Scientific reports7(1)7812(2017 Aug)
Nagasaka A,Mogi C,Ono H,Nishi T,Horii Y,Ohba Y,Sato K,Nakaya M,Okajima F,Kurose H

TDAG8 involved in initiating inflammatory hyperalgesia and establishing hyperalgesic priming in mice.
Scientific reports741415(2017 Feb)
Dai SP,Huang YH,Chang CJ,Huang YF,Hsieh WS,Tabata Y,Ishii S,Sun WH

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Gpr65 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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