Gja3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gja3
Entrez Gene ID	14611
Full Name	gap junction protein, alpha 3
Synonyms	Cnx46,Cx43,Cx46,Gja-3
General protein information	Preferred Names gap junction protein, alpha 3 Names gap junction alpha-3 protein alpha 3 connexin connexin-46 gap junction membrane channel protein alpha 3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14 C3\|14 29.82 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_011244959.1	XP_011243261.1	gap junction alpha-3 protein isoform X1
NM_016975.3	NP_058671.2	gap junction alpha-3 protein precursor
NM_001271623.1	NP_001258552.1	gap junction alpha-3 protein precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	GJA3	NP_068773.2
Gallus gallus (chicken)	GJA3	NP_001035734.1
Canis lupus familiaris (dog)	GJA3	XP_543178.2
Bos taurus (cattle)	GJA3	NP_001159768.1
Rattus norvegicus (Norway rat)	Gja3	NP_077352.1
Danio rerio (zebrafish)	gja3	NP_997525.1
Xenopus tropicalis (tropical clawed frog)	gja3	NP_001107721.1
Pan troglodytes (chimpanzee)	GJA3	XP_522616.2
	GJA3	XP_001087205.1
Mus musculus (house mouse)	Gja3	NP_001258552.1

Gene Ontology
Bibliography

Related articles in PubMed

Physiological and Optical Alterations Precede the Appearance of Cataracts in Cx46fs380 Mice.
Minogue PJ, Gao J, Zoltoski RK, Novak LA, Mathias RT, Beyer EC, Berthoud VM
Investigative ophthalmology & visual science58(10)4366?4374(2017 08)

Connexin 50 Regulates Surface Ball-and-Socket Structures and Fiber Cell Organization.
Wang E, Geng A, Maniar AM, Mui BW, Gong X
Investigative ophthalmology & visual science57(7)3039-46(2016 06)

Complementary expression and phosphorylation of Cx46 and Cx50 during development and following gene deletion in mouse and in normal and orchitic mink testes.
Pelletier RM, Akpovi CD, Chen L, Kumar NM, Vitale ML
American journal of physiology. Regulatory, integrative and comparative physiology309(3)R255-76(2015 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

that expression and phosphorylation of connexin 46 and connexin 45 are complementary in seminiferous tubules
Title: Complementary expression and phosphorylation of Cx46 and Cx50 during development and following gene deletion in mouse and in normal and orchitic mink testes.

The localization and sizes of Cx50 gap junction plaques in DKO fibers were unaffected, suggesting that Cx46 and Cx50 form homomeric channels
Title: Lens ion homeostasis relies on the assembly and/or stability of large connexin 46 gap junction plaques on the broad sides of differentiating fiber cells.

Data indicate that connexin 46 (cx46) is essential for the maintenance of adequate levels of glutathione (GSH) in the lens nucleus.
Title: Connexin 46 (cx46) gap junctions provide a pathway for the delivery of glutathione to the lens nucleus.

Cx46 was severely reduced in both heterozygous and homozygous Cx46fs380 lenses at 1 month of age, whereas immunoreactive connexin50 (Cx50) was moderately decreased.
Title: Connexin46fs380 causes progressive cataracts.

Results suggest that Cx46 hemichannels may contribute to the sodium leak conductance in lens fiber cells.
Title: Cx46 hemichannels contribute to the sodium leak conductance in lens fiber cells.

The following Gja3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gja3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu19319	NM_016975.3 Latest version!	Mus musculus gap junction protein, alpha 3 (Gja3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu19319	NM_001271623.1 Latest version!	Mus musculus gap junction protein, alpha 3 (Gja3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu19319	XM_011244959.1 Latest version!	Mus musculus gap junction protein, alpha 3 (Gja3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gja3 ( NM_016975.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_058671.2 Mus musculus gap junction protein, alpha 3 (Gja3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu19319
Clone ID Related Accession (Same CDS sequence)	XM_011244959.1 , NM_016975.3 , NM_001271623.1
Accession Version	NM_016975.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-16
Organism	Mus musculus(house mouse)
Product	gap junction alpha-3 protein precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC138492.1, AK136383.1 and BM125285.1. On Oct 27, 2012 this sequence version replaced NM_016975.2. Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC138492.1, AY390398.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849383 [ECO:0000348] ##Evidence-Data-END##

ATGGGCGACT GGAGCTTCCT GGGGCGGCTG CTGGAGAACG CACAGGAGCA CTCTACAGTC 
ATCGGCAAAG TGTGGCTGAC CGTGCTGTTC ATCTTCCGCA TTCTGGTGTT AGGGGCGGCA 
GCCGAGGAGG TGTGGGGCGA CGAGCAATCG GACTTCACCT GCAACACACA GCAGCCAGGC 
TGTGAGAACG TCTGCTACGA CCGCGCTTTC CCCATTTCGC ACATCCGCTT CTGGGCGCTG 
CAAATCATCT TCGTGTCTAC GCCCACCCTC ATCTATCTGG GCCACGTGCT ACACATCGTG 
CGCATGGAGG AGAAGAAGAA AGAGCGGGAG GAAGAGCTGC TGAGGAGAGA CAACCCTCAG 
CACGGCCGTG GTCGCGAGCC AATGCGTACA GGGAGCCCGC GGGACCCTCC ACTACGCGAT 
GACCGTGGCA AGGTGCGCAT CGCAGGTGCG CTGCTGCGGA CCTACGTCTT CAACATCATC 
TTCAAGACAC TCTTCGAAGT GGGGTTCATC GCGGGCCAGT ACTTTCTATA CGGCTTCCAG 
CTGCAGCCAC TTTACCGCTG CGACCGCTGG CCCTGCCCCA ACACTGTGGA CTGTTTCATC 
TCCAGGCCCA CAGAGAAGAC CATCTTTGTC ATCTTCATGC TGGCTGTGGC CTGTGCGTCA 
CTGGTACTCA ACATGCTGGA GATTTACCAC CTGGGCTGGA AGAAGCTCAA GCAGGGAGTT 
ACTAACCACT TCAACCCAGA TGCCTCAGAA GCCAGGCACA AGCCCTTGGA CCCCCTACCC 
ACGGCCACCA GCTCTGGCCC GCCCAGCGTC TCCATCGGGT TCCCACCTTA TTACACACAC 
CCTGCCTGTC CCACAGTACA GGCAAAGGCC ATAGGGTTTC CTGGGGCCCC ACTATCACCA 
GCAGACTTCA CAGTGGTGAC TCTAAACGAT GCTCAAGGCA GAAACCACCC AGTCAAACAC 
TGCAATGGCC ACCACCTGAC GACAGAGCAG AACTGGACCA GGCAAGTGGC AGAGCAGCAG 
ACTCCAGCCA GCAAGCCCTC TTCAGCAGCA TCCAGCCCTG ATGGCCGCAA GGGGCTCATT 
GACAGCAGTG GCAGCAGCTT ACAGGAGAGT GCCTTGGTAG TGACGCCAGA GGAGGGGGAA 
CAGGCTTTGG CCACCACAGT GGAGATGCAC TCGCCACCGT TGGTCCTCCT GGACCCAGGA 
AGGTCCAGCA AGTCCAGCAA CGGACGTGCC AGACCAGGTG ACTTGGCCAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058671.2
CDS	243..1496
Translation	MGDWSFLGRLLENAQEHSTVIGKVWLTVLFIFRILVLGAAAEEVWGDEQSDFTCNTQQPGCENVCYDRAFPISHIRFWALQIIFVSTPTLIYLGHVLHIVRMEEKKKEREEELLRRDNPQHGRGREPMRTGSPRDPPLRDDRGKVRIAGALLRTYVFNIIFKTLFEVGFIAGQYFLYGFQLQPLYRCDRWPCPNTVDCFISRPTEKTIFVIFMLAVACASLVLNMLEIYHLGWKKLKQGVTNHFNPDASEARHKPLDPLPTATSSGPPSVSIGFPPYYTHPACPTVQAKAIGFPGAPLSPADFTVVTLNDAQGRNHPVKHCNGHHLTTEQNWTRQVAEQQTPASKPSSAASSPDGRKGLIDSSGSSLQESALVVTPEEGEQALATTVEMHSPPLVLLDPGRSSKSSNGRARPGDLAI

Target ORF information:

RefSeq Version	NM_016975.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus gap junction protein, alpha 3 (Gja3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016975.3

ORF Insert Sequence:

ATGGGCGACT GGAGCTTCCT GGGGCGGCTG CTGGAGAACG CACAGGAGCA CTCTACAGTC 
ATCGGCAAAG TGTGGCTGAC CGTGCTGTTC ATCTTCCGCA TTCTGGTGTT AGGGGCGGCA 
GCCGAGGAGG TGTGGGGCGA CGAGCAATCG GACTTCACCT GCAACACACA GCAGCCAGGC 
TGTGAGAACG TCTGCTACGA CCGCGCTTTC CCCATTTCGC ACATCCGCTT CTGGGCGCTG 
CAAATCATCT TCGTGTCTAC GCCCACCCTC ATCTATCTGG GCCACGTGCT ACACATCGTG 
CGCATGGAGG AGAAGAAGAA AGAGCGGGAG GAAGAGCTGC TGAGGAGAGA CAACCCTCAG 
CACGGCCGTG GTCGCGAGCC AATGCGTACA GGGAGCCCGC GGGACCCTCC ACTACGCGAT 
GACCGTGGCA AGGTGCGCAT CGCAGGTGCG CTGCTGCGGA CCTACGTCTT CAACATCATC 
TTCAAGACAC TCTTCGAAGT GGGGTTCATC GCGGGCCAGT ACTTTCTATA CGGCTTCCAG 
CTGCAGCCAC TTTACCGCTG CGACCGCTGG CCCTGCCCCA ACACTGTGGA CTGTTTCATC 
TCCAGGCCCA CAGAGAAGAC CATCTTTGTC ATCTTCATGC TGGCTGTGGC CTGTGCGTCA 
CTGGTACTCA ACATGCTGGA GATTTACCAC CTGGGCTGGA AGAAGCTCAA GCAGGGAGTT 
ACTAACCACT TCAACCCAGA TGCCTCAGAA GCCAGGCACA AGCCCTTGGA CCCCCTACCC 
ACGGCCACCA GCTCTGGCCC GCCCAGCGTC TCCATCGGGT TCCCACCTTA TTACACACAC 
CCTGCCTGTC CCACAGTACA GGCAAAGGCC ATAGGGTTTC CTGGGGCCCC ACTATCACCA 
GCAGACTTCA CAGTGGTGAC TCTAAACGAT GCTCAAGGCA GAAACCACCC AGTCAAACAC 
TGCAATGGCC ACCACCTGAC GACAGAGCAG AACTGGACCA GGCAAGTGGC AGAGCAGCAG 
ACTCCAGCCA GCAAGCCCTC TTCAGCAGCA TCCAGCCCTG ATGGCCGCAA GGGGCTCATT 
GACAGCAGTG GCAGCAGCTT ACAGGAGAGT GCCTTGGTAG TGACGCCAGA GGAGGGGGAA 
CAGGCTTTGG CCACCACAGT GGAGATGCAC TCGCCACCGT TGGTCCTCCT GGACCCAGGA 
AGGTCCAGCA AGTCCAGCAA CGGACGTGCC AGACCAGGTG ACTTGGCCAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gja3 ( NM_001271623.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001258552.1 Mus musculus gap junction protein, alpha 3 (Gja3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu19319
Clone ID Related Accession (Same CDS sequence)	XM_011244959.1 , NM_016975.3 , NM_001271623.1
Accession Version	NM_001271623.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-16
Organism	Mus musculus(house mouse)
Product	gap junction alpha-3 protein precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC119952.12, BQ554641.1, BC138492.1, AK136383.1 and BM125285.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY390399.1, FO719742.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383, SAMN00849385 [ECO:0000348] ##Evidence-Data-END##

ATGGGCGACT GGAGCTTCCT GGGGCGGCTG CTGGAGAACG CACAGGAGCA CTCTACAGTC 
ATCGGCAAAG TGTGGCTGAC CGTGCTGTTC ATCTTCCGCA TTCTGGTGTT AGGGGCGGCA 
GCCGAGGAGG TGTGGGGCGA CGAGCAATCG GACTTCACCT GCAACACACA GCAGCCAGGC 
TGTGAGAACG TCTGCTACGA CCGCGCTTTC CCCATTTCGC ACATCCGCTT CTGGGCGCTG 
CAAATCATCT TCGTGTCTAC GCCCACCCTC ATCTATCTGG GCCACGTGCT ACACATCGTG 
CGCATGGAGG AGAAGAAGAA AGAGCGGGAG GAAGAGCTGC TGAGGAGAGA CAACCCTCAG 
CACGGCCGTG GTCGCGAGCC AATGCGTACA GGGAGCCCGC GGGACCCTCC ACTACGCGAT 
GACCGTGGCA AGGTGCGCAT CGCAGGTGCG CTGCTGCGGA CCTACGTCTT CAACATCATC 
TTCAAGACAC TCTTCGAAGT GGGGTTCATC GCGGGCCAGT ACTTTCTATA CGGCTTCCAG 
CTGCAGCCAC TTTACCGCTG CGACCGCTGG CCCTGCCCCA ACACTGTGGA CTGTTTCATC 
TCCAGGCCCA CAGAGAAGAC CATCTTTGTC ATCTTCATGC TGGCTGTGGC CTGTGCGTCA 
CTGGTACTCA ACATGCTGGA GATTTACCAC CTGGGCTGGA AGAAGCTCAA GCAGGGAGTT 
ACTAACCACT TCAACCCAGA TGCCTCAGAA GCCAGGCACA AGCCCTTGGA CCCCCTACCC 
ACGGCCACCA GCTCTGGCCC GCCCAGCGTC TCCATCGGGT TCCCACCTTA TTACACACAC 
CCTGCCTGTC CCACAGTACA GGCAAAGGCC ATAGGGTTTC CTGGGGCCCC ACTATCACCA 
GCAGACTTCA CAGTGGTGAC TCTAAACGAT GCTCAAGGCA GAAACCACCC AGTCAAACAC 
TGCAATGGCC ACCACCTGAC GACAGAGCAG AACTGGACCA GGCAAGTGGC AGAGCAGCAG 
ACTCCAGCCA GCAAGCCCTC TTCAGCAGCA TCCAGCCCTG ATGGCCGCAA GGGGCTCATT 
GACAGCAGTG GCAGCAGCTT ACAGGAGAGT GCCTTGGTAG TGACGCCAGA GGAGGGGGAA 
CAGGCTTTGG CCACCACAGT GGAGATGCAC TCGCCACCGT TGGTCCTCCT GGACCCAGGA 
AGGTCCAGCA AGTCCAGCAA CGGACGTGCC AGACCAGGTG ACTTGGCCAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258552.1
CDS	215..1468
Translation	MGDWSFLGRLLENAQEHSTVIGKVWLTVLFIFRILVLGAAAEEVWGDEQSDFTCNTQQPGCENVCYDRAFPISHIRFWALQIIFVSTPTLIYLGHVLHIVRMEEKKKEREEELLRRDNPQHGRGREPMRTGSPRDPPLRDDRGKVRIAGALLRTYVFNIIFKTLFEVGFIAGQYFLYGFQLQPLYRCDRWPCPNTVDCFISRPTEKTIFVIFMLAVACASLVLNMLEIYHLGWKKLKQGVTNHFNPDASEARHKPLDPLPTATSSGPPSVSIGFPPYYTHPACPTVQAKAIGFPGAPLSPADFTVVTLNDAQGRNHPVKHCNGHHLTTEQNWTRQVAEQQTPASKPSSAASSPDGRKGLIDSSGSSLQESALVVTPEEGEQALATTVEMHSPPLVLLDPGRSSKSSNGRARPGDLAI

Target ORF information:

RefSeq Version	NM_001271623.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus gap junction protein, alpha 3 (Gja3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271623.1

ORF Insert Sequence:

ATGGGCGACT GGAGCTTCCT GGGGCGGCTG CTGGAGAACG CACAGGAGCA CTCTACAGTC 
ATCGGCAAAG TGTGGCTGAC CGTGCTGTTC ATCTTCCGCA TTCTGGTGTT AGGGGCGGCA 
GCCGAGGAGG TGTGGGGCGA CGAGCAATCG GACTTCACCT GCAACACACA GCAGCCAGGC 
TGTGAGAACG TCTGCTACGA CCGCGCTTTC CCCATTTCGC ACATCCGCTT CTGGGCGCTG 
CAAATCATCT TCGTGTCTAC GCCCACCCTC ATCTATCTGG GCCACGTGCT ACACATCGTG 
CGCATGGAGG AGAAGAAGAA AGAGCGGGAG GAAGAGCTGC TGAGGAGAGA CAACCCTCAG 
CACGGCCGTG GTCGCGAGCC AATGCGTACA GGGAGCCCGC GGGACCCTCC ACTACGCGAT 
GACCGTGGCA AGGTGCGCAT CGCAGGTGCG CTGCTGCGGA CCTACGTCTT CAACATCATC 
TTCAAGACAC TCTTCGAAGT GGGGTTCATC GCGGGCCAGT ACTTTCTATA CGGCTTCCAG 
CTGCAGCCAC TTTACCGCTG CGACCGCTGG CCCTGCCCCA ACACTGTGGA CTGTTTCATC 
TCCAGGCCCA CAGAGAAGAC CATCTTTGTC ATCTTCATGC TGGCTGTGGC CTGTGCGTCA 
CTGGTACTCA ACATGCTGGA GATTTACCAC CTGGGCTGGA AGAAGCTCAA GCAGGGAGTT 
ACTAACCACT TCAACCCAGA TGCCTCAGAA GCCAGGCACA AGCCCTTGGA CCCCCTACCC 
ACGGCCACCA GCTCTGGCCC GCCCAGCGTC TCCATCGGGT TCCCACCTTA TTACACACAC 
CCTGCCTGTC CCACAGTACA GGCAAAGGCC ATAGGGTTTC CTGGGGCCCC ACTATCACCA 
GCAGACTTCA CAGTGGTGAC TCTAAACGAT GCTCAAGGCA GAAACCACCC AGTCAAACAC 
TGCAATGGCC ACCACCTGAC GACAGAGCAG AACTGGACCA GGCAAGTGGC AGAGCAGCAG 
ACTCCAGCCA GCAAGCCCTC TTCAGCAGCA TCCAGCCCTG ATGGCCGCAA GGGGCTCATT 
GACAGCAGTG GCAGCAGCTT ACAGGAGAGT GCCTTGGTAG TGACGCCAGA GGAGGGGGAA 
CAGGCTTTGG CCACCACAGT GGAGATGCAC TCGCCACCGT TGGTCCTCCT GGACCCAGGA 
AGGTCCAGCA AGTCCAGCAA CGGACGTGCC AGACCAGGTG ACTTGGCCAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gja3 ( XM_011244959.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011243261.1 Mus musculus gap junction protein, alpha 3 (Gja3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu19319
Clone ID Related Accession (Same CDS sequence)	XM_011244959.1 , NM_016975.3 , NM_001271623.1
Accession Version	XM_011244959.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	gap junction alpha-3 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACT GGAGCTTCCT GGGGCGGCTG CTGGAGAACG CACAGGAGCA CTCTACAGTC 
ATCGGCAAAG TGTGGCTGAC CGTGCTGTTC ATCTTCCGCA TTCTGGTGTT AGGGGCGGCA 
GCCGAGGAGG TGTGGGGCGA CGAGCAATCG GACTTCACCT GCAACACACA GCAGCCAGGC 
TGTGAGAACG TCTGCTACGA CCGCGCTTTC CCCATTTCGC ACATCCGCTT CTGGGCGCTG 
CAAATCATCT TCGTGTCTAC GCCCACCCTC ATCTATCTGG GCCACGTGCT ACACATCGTG 
CGCATGGAGG AGAAGAAGAA AGAGCGGGAG GAAGAGCTGC TGAGGAGAGA CAACCCTCAG 
CACGGCCGTG GTCGCGAGCC AATGCGTACA GGGAGCCCGC GGGACCCTCC ACTACGCGAT 
GACCGTGGCA AGGTGCGCAT CGCAGGTGCG CTGCTGCGGA CCTACGTCTT CAACATCATC 
TTCAAGACAC TCTTCGAAGT GGGGTTCATC GCGGGCCAGT ACTTTCTATA CGGCTTCCAG 
CTGCAGCCAC TTTACCGCTG CGACCGCTGG CCCTGCCCCA ACACTGTGGA CTGTTTCATC 
TCCAGGCCCA CAGAGAAGAC CATCTTTGTC ATCTTCATGC TGGCTGTGGC CTGTGCGTCA 
CTGGTACTCA ACATGCTGGA GATTTACCAC CTGGGCTGGA AGAAGCTCAA GCAGGGAGTT 
ACTAACCACT TCAACCCAGA TGCCTCAGAA GCCAGGCACA AGCCCTTGGA CCCCCTACCC 
ACGGCCACCA GCTCTGGCCC GCCCAGCGTC TCCATCGGGT TCCCACCTTA TTACACACAC 
CCTGCCTGTC CCACAGTACA GGCAAAGGCC ATAGGGTTTC CTGGGGCCCC ACTATCACCA 
GCAGACTTCA CAGTGGTGAC TCTAAACGAT GCTCAAGGCA GAAACCACCC AGTCAAACAC 
TGCAATGGCC ACCACCTGAC GACAGAGCAG AACTGGACCA GGCAAGTGGC AGAGCAGCAG 
ACTCCAGCCA GCAAGCCCTC TTCAGCAGCA TCCAGCCCTG ATGGCCGCAA GGGGCTCATT 
GACAGCAGTG GCAGCAGCTT ACAGGAGAGT GCCTTGGTAG TGACGCCAGA GGAGGGGGAA 
CAGGCTTTGG CCACCACAGT GGAGATGCAC TCGCCACCGT TGGTCCTCCT GGACCCAGGA 
AGGTCCAGCA AGTCCAGCAA CGGACGTGCC AGACCAGGTG ACTTGGCCAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011243261.1
CDS	263..1516
Translation	MGDWSFLGRLLENAQEHSTVIGKVWLTVLFIFRILVLGAAAEEVWGDEQSDFTCNTQQPGCENVCYDRAFPISHIRFWALQIIFVSTPTLIYLGHVLHIVRMEEKKKEREEELLRRDNPQHGRGREPMRTGSPRDPPLRDDRGKVRIAGALLRTYVFNIIFKTLFEVGFIAGQYFLYGFQLQPLYRCDRWPCPNTVDCFISRPTEKTIFVIFMLAVACASLVLNMLEIYHLGWKKLKQGVTNHFNPDASEARHKPLDPLPTATSSGPPSVSIGFPPYYTHPACPTVQAKAIGFPGAPLSPADFTVVTLNDAQGRNHPVKHCNGHHLTTEQNWTRQVAEQQTPASKPSSAASSPDGRKGLIDSSGSSLQESALVVTPEEGEQALATTVEMHSPPLVLLDPGRSSKSSNGRARPGDLAI

Target ORF information:

RefSeq Version	XM_011244959.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus gap junction protein, alpha 3 (Gja3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011244959.1

ORF Insert Sequence:

ATGGGCGACT GGAGCTTCCT GGGGCGGCTG CTGGAGAACG CACAGGAGCA CTCTACAGTC 
ATCGGCAAAG TGTGGCTGAC CGTGCTGTTC ATCTTCCGCA TTCTGGTGTT AGGGGCGGCA 
GCCGAGGAGG TGTGGGGCGA CGAGCAATCG GACTTCACCT GCAACACACA GCAGCCAGGC 
TGTGAGAACG TCTGCTACGA CCGCGCTTTC CCCATTTCGC ACATCCGCTT CTGGGCGCTG 
CAAATCATCT TCGTGTCTAC GCCCACCCTC ATCTATCTGG GCCACGTGCT ACACATCGTG 
CGCATGGAGG AGAAGAAGAA AGAGCGGGAG GAAGAGCTGC TGAGGAGAGA CAACCCTCAG 
CACGGCCGTG GTCGCGAGCC AATGCGTACA GGGAGCCCGC GGGACCCTCC ACTACGCGAT 
GACCGTGGCA AGGTGCGCAT CGCAGGTGCG CTGCTGCGGA CCTACGTCTT CAACATCATC 
TTCAAGACAC TCTTCGAAGT GGGGTTCATC GCGGGCCAGT ACTTTCTATA CGGCTTCCAG 
CTGCAGCCAC TTTACCGCTG CGACCGCTGG CCCTGCCCCA ACACTGTGGA CTGTTTCATC 
TCCAGGCCCA CAGAGAAGAC CATCTTTGTC ATCTTCATGC TGGCTGTGGC CTGTGCGTCA 
CTGGTACTCA ACATGCTGGA GATTTACCAC CTGGGCTGGA AGAAGCTCAA GCAGGGAGTT 
ACTAACCACT TCAACCCAGA TGCCTCAGAA GCCAGGCACA AGCCCTTGGA CCCCCTACCC 
ACGGCCACCA GCTCTGGCCC GCCCAGCGTC TCCATCGGGT TCCCACCTTA TTACACACAC 
CCTGCCTGTC CCACAGTACA GGCAAAGGCC ATAGGGTTTC CTGGGGCCCC ACTATCACCA 
GCAGACTTCA CAGTGGTGAC TCTAAACGAT GCTCAAGGCA GAAACCACCC AGTCAAACAC 
TGCAATGGCC ACCACCTGAC GACAGAGCAG AACTGGACCA GGCAAGTGGC AGAGCAGCAG 
ACTCCAGCCA GCAAGCCCTC TTCAGCAGCA TCCAGCCCTG ATGGCCGCAA GGGGCTCATT 
GACAGCAGTG GCAGCAGCTT ACAGGAGAGT GCCTTGGTAG TGACGCCAGA GGAGGGGGAA 
CAGGCTTTGG CCACCACAGT GGAGATGCAC TCGCCACCGT TGGTCCTCCT GGACCCAGGA 
AGGTCCAGCA AGTCCAGCAA CGGACGTGCC AGACCAGGTG ACTTGGCCAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Physiological and Optical Alterations Precede the Appearance of Cataracts in Cx46fs380 Mice.
Investigative ophthalmology & visual science58(10)4366?4374(2017 08)
Minogue PJ,Gao J,Zoltoski RK,Novak LA,Mathias RT,Beyer EC,Berthoud VM

Connexin 50 Regulates Surface Ball-and-Socket Structures and Fiber Cell Organization.
Investigative ophthalmology & visual science57(7)3039-46(2016 06)
Wang E,Geng A,Maniar AM,Mui BW,Gong X

Complementary expression and phosphorylation of Cx46 and Cx50 during development and following gene deletion in mouse and in normal and orchitic mink testes.
American journal of physiology. Regulatory, integrative and comparative physiology309(3)R255-76(2015 Aug)
Pelletier RM,Akpovi CD,Chen L,Kumar NM,Vitale ML

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Gja3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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