Fut1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fut1
Entrez Gene ID	14343
Full Name	fucosyltransferase 1
General protein information	Preferred Names fucosyltransferase 1 Names galactoside 2-alpha-L-fucosyltransferase 1 GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase 1 H transferase alpha(1,2)FT 1 beta-galactoside alpha-1,2-fucosyltransferase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7 B3\|7 29.39 cM
Summary	This gene is one of three genes in mouse which encode a galactoside 2-L-fucosyltransferase. These genes differ in their developmental- and tissue-specific expression. The encoded type II membrane protein is anchored in the Golgi apparatus and controls the final step in the creation of alpha (1,2) fucosylated carbhohydrates by the addition of a terminal fucose in an alpha (1,2) linkage. This enzyme is required for the synthesis of the Lewis antigen as well as the H-antigen, a precursor of the A and B antigens of the ABH histo-blood group. The biological function of the fucosylated carbhohydrate products is thought to involve cell-adhesion and interactions with microorganisms. Disruption of this gene impairs development of the olfactory nerve and maturation of the glomerular layer of the main olfactory bulb. Alternative splicing results in multiple transcript variants which encode distinct isoforms. [provided by RefSeq, Dec 2012].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006540616.3	XP_006540679.1	galactoside 2-alpha-L-fucosyltransferase 1 isoform X1
NM_001271981.1	NP_001258910.1	galactoside 2-alpha-L-fucosyltransferase 1 isoform 2
NM_008051.6	NP_032077.2	galactoside 2-alpha-L-fucosyltransferase 1 isoform 1

Pathways from BioSystems

Homologies

	FUT1	NP_001040619.1
Canis lupus familiaris (dog)	FUT1	XP_005616392.1
Rattus norvegicus (Norway rat)	Fut1	NP_112515.1
Xenopus tropicalis (tropical clawed frog)	fut1	NP_001004772.1
Homo sapiens (human)	FUT1	NP_000139.1
Pan troglodytes (chimpanzee)	FUT1	NP_001009121.1
Bos taurus (cattle)	FUT1	NP_803465.2
Mus musculus (house mouse)	Fut1	NP_032077.2

Gene Ontology
Bibliography

Related articles in PubMed

miR-339-5p downregulation contributes to Taxol resistance in small-cell lung cancer by targeting ?1,2-fucosyltransferase 1.
Gan CZ, Li G, Luo QS, Li HM
IUBMB life69(11)841-849(2017 Nov)

A key role for Fut1-regulated angiogenesis and ICAM-1 expression in K/BxN arthritis.
Amin MA, Campbell PL, Ruth JH, Isozaki T, Rabquer BJ, Alex Stinson W, O'Brien M, Edhayan G, Ohara RA, Vargo J, Domino SE, Koch AE
Annals of the rheumatic diseases74(7)1459-66(2015 Jul)

Different effects of androgen on the expression of Fut1, Fut2, Fut4 and Fut9 in male mouse reproductive tract.
Wang CM, Hu SG, Ru YF, Yao GX, Ma WB, Gu YH, Chu C, Wang SL, Zhou ZM, Liu Q, Zhou YC, Zhang YL
International journal of molecular sciences14(11)23188-202(2013 Nov)

A phenotype survey of 36 mutant mouse strains with gene-targeted defects in glycosyltransferases or glycan-binding proteins.
Orr SL, Le D, Long JM, Sobieszczuk P, Ma B, Tian H, Fang X, Paulson JC, Marth JD, Varki N
Glycobiology23(3)363-80(2013 Mar)

Distinct fucosylation of M cells and epithelial cells by Fut1 and Fut2, respectively, in response to intestinal environmental stress.
Terahara K, Nochi T, Yoshida M, Takahashi Y, Goto Y, Hatai H, Kurokawa S, Jang MH, Kweon MN, Domino SE, Hiroi T, Yuki Y, Tsunetsugu-Yokota Y, Kobayashi K, Kiyono H
Biochemical and biophysical research communications404(3)822-8(2011 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Fut1 plays an important role in regulating angiogenesis and ICAM-1 expression in inflammatory arthritis.
Title: A key role for Fut1-regulated angiogenesis and ICAM-1 expression in K/BxN arthritis.

We found that Fut1 mRNA and Fut4 mRNA were upregulated, while Fut2 mRNA and Fut9 mRNA were downregulated by androgen in the caput epididymis.
Title: Different effects of androgen on the expression of Fut1, Fut2, Fut4 and Fut9 in male mouse reproductive tract.

fucosyltransferase (Fut)-deficient (Fut1, Fut2 and Fut4) mice which lack LeX or LeY antigen are still fertile.
Title: Biosynthesis and distribution of Lewis X- and Lewis Y-containing glycoproteins in the murine male reproductive system.

These results indicate that Fut2-mediated fucosylated epithelial cells (F-ECs) share M cell-related fucosylated molecules but maintain distinctive EC characteristics, Fut1 is, therefore, a reliable marker for M cells.
Title: Distinct fucosylation of M cells and epithelial cells by Fut1 and Fut2, respectively, in response to intestinal environmental stress.

Vasculogenesis was the most clearly affected by FUT1 expression, suggesting that tumor angiomorphogenesis may depend on E-selectin-mediated interaction between HCC and endothelial cells, the inhibition of which remarkably retards tumor growth.
Title: Introducing alpha(1,2)-linked fucose into hepatocarcinoma cells inhibits vasculogenesis and tumor growth.

The following Fut1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fut1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu07428	NM_001271981.1 Latest version!	Mus musculus fucosyltransferase 1 (Fut1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu01285	NM_008051.6 Latest version!	Mus musculus fucosyltransferase 1 (Fut1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu01285	XM_006540616.3 Latest version!	Mus musculus fucosyltransferase 1 (Fut1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fut1 ( NM_001271981.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001258910.1 Mus musculus fucosyltransferase 1 (Fut1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu07428
Clone ID Related Accession (Same CDS sequence)	NM_001271981.1
Accession Version	NM_001271981.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	galactoside 2-alpha-L-fucosyltransferase 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK137638.1, BC109145.1 and AC149057.7. Transcript Variant: This variant (2) uses an alternate splice site at the 5' end of the coding exon and uses an in-frame downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC109145.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849383 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTATGCC TACCTCATCC ATTGCAGACA TCTAATGGCT CCCCTTCCTG TCCTGAGCAG 
TCCTCCTCAC TCTCTGGGAC TTGGACAATC ACCCCAGGAG GCAGGTTTGG TAACCAGATG 
GGGCAGTATG CTACATTGCT GGCCCTAGCC CAGCTCAATG GTCGCCAAGC CTTCATCCAA 
CCTGAGATGC ATGCCGCCCT GGCCCCCGTG TTCCGAATCT CCCTGCCAGT GCTGGACCCT 
GAGGTGGACA GCCTCACACC TTGGCAGCAC TTAGTCCTAC ATGACTGGAT GTCAGAGGAG 
TACTCCCATC TGGAGGACCC ATTTCTCAAG CTGTCTGGTT TCCCCTGCTC TTGGACCTTT 
TTCCATCATC TTCGGGAACA GATTCGTAGG GAATTCACTC TGCATAACCA TCTACGGGAA 
GGTGCCCAGT ACCTGTTGAG CGGGCTCCGT ATAGGCCCGG CGGGCATCCG CCCTCATACC 
TTTGTGGGTG TCCATGTGCG TCGTGGAGAC TATCTGGAGG TGATGCCCAA TCGCTGGAAG 
GGTGTGGTGG GTGACCGAGC TTACCTCCAG CAAGCCATGG ACTGGTTCCG GGCCCGACAC 
AAAGACCCCA TCTTTGTGGT CACCAGCAAT GGCATGAAAT GGTGTTTGGA GAACATTGAC 
ACATCCCATG GTGATGTGGT CTTCGCTGGC AATGGACAGG AGGGTACACC GGGGAAGGAC 
TTTGCACTTC TCACACAGTG TAACCACACC ATCATGACTA TTGGCACCTT TGGCTTCTGG 
GCTGCCTACT TAGCTGGTGG AGACACGGTC TACCTTGCAA ACTTCACCCT GCCAGATTCG 
GAGTTTCTGA AGATCTTCAG GCCTGAGGCT GCCTTCCTGC CTGAGTGGGT GGGCATCAAT 
GCAGACTTGT CCCCGCTGCA GGCTCAATTT GACCCCTGGA AGCCAGACAG TCTTTTTAGA 
TTGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258910.1
CDS	172..1140
Translation	MVCLPHPLQTSNGSPSCPEQSSSLSGTWTITPGGRFGNQMGQYATLLALAQLNGRQAFIQPEMHAALAPVFRISLPVLDPEVDSLTPWQHLVLHDWMSEEYSHLEDPFLKLSGFPCSWTFFHHLREQIRREFTLHNHLREGAQYLLSGLRIGPAGIRPHTFVGVHVRRGDYLEVMPNRWKGVVGDRAYLQQAMDWFRARHKDPIFVVTSNGMKWCLENIDTSHGDVVFAGNGQEGTPGKDFALLTQCNHTIMTIGTFGFWAAYLAGGDTVYLANFTLPDSEFLKIFRPEAAFLPEWVGINADLSPLQAQFDPWKPDSLFRLV

Target ORF information:

RefSeq Version	NM_001271981.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus fucosyltransferase 1 (Fut1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271981.1

ORF Insert Sequence:

ATGGTATGCC TACCTCATCC ATTGCAGACA TCTAATGGCT CCCCTTCCTG TCCTGAGCAG 
TCCTCCTCAC TCTCTGGGAC TTGGACAATC ACCCCAGGAG GCAGGTTTGG TAACCAGATG 
GGGCAGTATG CTACATTGCT GGCCCTAGCC CAGCTCAATG GTCGCCAAGC CTTCATCCAA 
CCTGAGATGC ATGCCGCCCT GGCCCCCGTG TTCCGAATCT CCCTGCCAGT GCTGGACCCT 
GAGGTGGACA GCCTCACACC TTGGCAGCAC TTAGTCCTAC ATGACTGGAT GTCAGAGGAG 
TACTCCCATC TGGAGGACCC ATTTCTCAAG CTGTCTGGTT TCCCCTGCTC TTGGACCTTT 
TTCCATCATC TTCGGGAACA GATTCGTAGG GAATTCACTC TGCATAACCA TCTACGGGAA 
GGTGCCCAGT ACCTGTTGAG CGGGCTCCGT ATAGGCCCGG CGGGCATCCG CCCTCATACC 
TTTGTGGGTG TCCATGTGCG TCGTGGAGAC TATCTGGAGG TGATGCCCAA TCGCTGGAAG 
GGTGTGGTGG GTGACCGAGC TTACCTCCAG CAAGCCATGG ACTGGTTCCG GGCCCGACAC 
AAAGACCCCA TCTTTGTGGT CACCAGCAAT GGCATGAAAT GGTGTTTGGA GAACATTGAC 
ACATCCCATG GTGATGTGGT CTTCGCTGGC AATGGACAGG AGGGTACACC GGGGAAGGAC 
TTTGCACTTC TCACACAGTG TAACCACACC ATCATGACTA TTGGCACCTT TGGCTTCTGG 
GCTGCCTACT TAGCTGGTGG AGACACGGTC TACCTTGCAA ACTTCACCCT GCCAGATTCG 
GAGTTTCTGA AGATCTTCAG GCCTGAGGCT GCCTTCCTGC CTGAGTGGGT GGGCATCAAT 
GCAGACTTGT CCCCGCTGCA GGCTCAATTT GACCCCTGGA AGCCAGACAG TCTTTTTAGA 
TTGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fut1 ( NM_008051.6 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032077.2 Mus musculus fucosyltransferase 1 (Fut1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu01285
Clone ID Related Accession (Same CDS sequence)	XM_006540616.3 , NM_008051.6
Accession Version	NM_008051.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	galactoside 2-alpha-L-fucosyltransferase 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK137638.1 and AC149057.7. On Dec 20, 2012 this sequence version replaced NM_008051.5. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note:This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK137638.1, CJ056234.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGACTC CCAGCCGGAG GCAGCTCTGC CTGGCATTTC TGTTGGTCTG TGTGCTCTCT 
GCCGGCTCCT TCTTTTTCCA CCTGAATGGA GGAAACTTCT TTCGAAATGG TCTAACTCTC 
TCTGTCCTGT GTTCAGACTA TCACCTGCTG AAGTCCCCAG TGGCCATGGT ATGCCTACCT 
CATCCATTGC AGACATCTAA TGGCTCCCCT TCCTGTCCTG AGCAGTCCTC CTCACTCTCT 
GGGACTTGGA CAATCACCCC AGGAGGCAGG TTTGGTAACC AGATGGGGCA GTATGCTACA 
TTGCTGGCCC TAGCCCAGCT CAATGGTCGC CAAGCCTTCA TCCAACCTGA GATGCATGCC 
GCCCTGGCCC CCGTGTTCCG AATCTCCCTG CCAGTGCTGG ACCCTGAGGT GGACAGCCTC 
ACACCTTGGC AGCACTTAGT CCTACATGAC TGGATGTCAG AGGAGTACTC CCATCTGGAG 
GACCCATTTC TCAAGCTGTC TGGTTTCCCC TGCTCTTGGA CCTTTTTCCA TCATCTTCGG 
GAACAGATTC GTAGGGAATT CACTCTGCAT AACCATCTAC GGGAAGGTGC CCAGTACCTG 
TTGAGCGGGC TCCGTATAGG CCCGGCGGGC ATCCGCCCTC ATACCTTTGT GGGTGTCCAT 
GTGCGTCGTG GAGACTATCT GGAGGTGATG CCCAATCGCT GGAAGGGTGT GGTGGGTGAC 
CGAGCTTACC TCCAGCAAGC CATGGACTGG TTCCGGGCCC GACACAAAGA CCCCATCTTT 
GTGGTCACCA GCAATGGCAT GAAATGGTGT TTGGAGAACA TTGACACATC CCATGGTGAT 
GTGGTCTTCG CTGGCAATGG ACAGGAGGGT ACACCGGGGA AGGACTTTGC ACTTCTCACA 
CAGTGTAACC ACACCATCAT GACTATTGGC ACCTTTGGCT TCTGGGCTGC CTACTTAGCT 
GGTGGAGACA CGGTCTACCT TGCAAACTTC ACCCTGCCAG ATTCGGAGTT TCTGAAGATC 
TTCAGGCCTG AGGCTGCCTT CCTGCCTGAG TGGGTGGGCA TCAATGCAGA CTTGTCCCCG 
CTGCAGGCTC AATTTGACCC CTGGAAGCCA GACAGTCTTT TTAGATTGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032077.2
CDS	145..1278
Translation	MWTPSRRQLCLAFLLVCVLSAGSFFFHLNGGNFFRNGLTLSVLCSDYHLLKSPVAMVCLPHPLQTSNGSPSCPEQSSSLSGTWTITPGGRFGNQMGQYATLLALAQLNGRQAFIQPEMHAALAPVFRISLPVLDPEVDSLTPWQHLVLHDWMSEEYSHLEDPFLKLSGFPCSWTFFHHLREQIRREFTLHNHLREGAQYLLSGLRIGPAGIRPHTFVGVHVRRGDYLEVMPNRWKGVVGDRAYLQQAMDWFRARHKDPIFVVTSNGMKWCLENIDTSHGDVVFAGNGQEGTPGKDFALLTQCNHTIMTIGTFGFWAAYLAGGDTVYLANFTLPDSEFLKIFRPEAAFLPEWVGINADLSPLQAQFDPWKPDSLFRLV

Target ORF information:

RefSeq Version	NM_008051.6
Organism	Mus musculus(house mouse)
Definition	Mus musculus fucosyltransferase 1 (Fut1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008051.6

ORF Insert Sequence:

ATGTGGACTC CCAGCCGGAG GCAGCTCTGC CTGGCATTTC TGTTGGTCTG TGTGCTCTCT 
GCCGGCTCCT TCTTTTTCCA CCTGAATGGA GGAAACTTCT TTCGAAATGG TCTAACTCTC 
TCTGTCCTGT GTTCAGACTA TCACCTGCTG AAGTCCCCAG TGGCCATGGT ATGCCTACCT 
CATCCATTGC AGACATCTAA TGGCTCCCCT TCCTGTCCTG AGCAGTCCTC CTCACTCTCT 
GGGACTTGGA CAATCACCCC AGGAGGCAGG TTTGGTAACC AGATGGGGCA GTATGCTACA 
TTGCTGGCCC TAGCCCAGCT CAATGGTCGC CAAGCCTTCA TCCAACCTGA GATGCATGCC 
GCCCTGGCCC CCGTGTTCCG AATCTCCCTG CCAGTGCTGG ACCCTGAGGT GGACAGCCTC 
ACACCTTGGC AGCACTTAGT CCTACATGAC TGGATGTCAG AGGAGTACTC CCATCTGGAG 
GACCCATTTC TCAAGCTGTC TGGTTTCCCC TGCTCTTGGA CCTTTTTCCA TCATCTTCGG 
GAACAGATTC GTAGGGAATT CACTCTGCAT AACCATCTAC GGGAAGGTGC CCAGTACCTG 
TTGAGCGGGC TCCGTATAGG CCCGGCGGGC ATCCGCCCTC ATACCTTTGT GGGTGTCCAT 
GTGCGTCGTG GAGACTATCT GGAGGTGATG CCCAATCGCT GGAAGGGTGT GGTGGGTGAC 
CGAGCTTACC TCCAGCAAGC CATGGACTGG TTCCGGGCCC GACACAAAGA CCCCATCTTT 
GTGGTCACCA GCAATGGCAT GAAATGGTGT TTGGAGAACA TTGACACATC CCATGGTGAT 
GTGGTCTTCG CTGGCAATGG ACAGGAGGGT ACACCGGGGA AGGACTTTGC ACTTCTCACA 
CAGTGTAACC ACACCATCAT GACTATTGGC ACCTTTGGCT TCTGGGCTGC CTACTTAGCT 
GGTGGAGACA CGGTCTACCT TGCAAACTTC ACCCTGCCAG ATTCGGAGTT TCTGAAGATC 
TTCAGGCCTG AGGCTGCCTT CCTGCCTGAG TGGGTGGGCA TCAATGCAGA CTTGTCCCCG 
CTGCAGGCTC AATTTGACCC CTGGAAGCCA GACAGTCTTT TTAGATTGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fut1 ( XM_006540616.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540679.1 Mus musculus fucosyltransferase 1 (Fut1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu01285
Clone ID Related Accession (Same CDS sequence)	XM_006540616.3 , NM_008051.6
Accession Version	XM_006540616.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	galactoside 2-alpha-L-fucosyltransferase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006540616.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGACTC CCAGCCGGAG GCAGCTCTGC CTGGCATTTC TGTTGGTCTG TGTGCTCTCT 
GCCGGCTCCT TCTTTTTCCA CCTGAATGGA GGAAACTTCT TTCGAAATGG TCTAACTCTC 
TCTGTCCTGT GTTCAGACTA TCACCTGCTG AAGTCCCCAG TGGCCATGGT ATGCCTACCT 
CATCCATTGC AGACATCTAA TGGCTCCCCT TCCTGTCCTG AGCAGTCCTC CTCACTCTCT 
GGGACTTGGA CAATCACCCC AGGAGGCAGG TTTGGTAACC AGATGGGGCA GTATGCTACA 
TTGCTGGCCC TAGCCCAGCT CAATGGTCGC CAAGCCTTCA TCCAACCTGA GATGCATGCC 
GCCCTGGCCC CCGTGTTCCG AATCTCCCTG CCAGTGCTGG ACCCTGAGGT GGACAGCCTC 
ACACCTTGGC AGCACTTAGT CCTACATGAC TGGATGTCAG AGGAGTACTC CCATCTGGAG 
GACCCATTTC TCAAGCTGTC TGGTTTCCCC TGCTCTTGGA CCTTTTTCCA TCATCTTCGG 
GAACAGATTC GTAGGGAATT CACTCTGCAT AACCATCTAC GGGAAGGTGC CCAGTACCTG 
TTGAGCGGGC TCCGTATAGG CCCGGCGGGC ATCCGCCCTC ATACCTTTGT GGGTGTCCAT 
GTGCGTCGTG GAGACTATCT GGAGGTGATG CCCAATCGCT GGAAGGGTGT GGTGGGTGAC 
CGAGCTTACC TCCAGCAAGC CATGGACTGG TTCCGGGCCC GACACAAAGA CCCCATCTTT 
GTGGTCACCA GCAATGGCAT GAAATGGTGT TTGGAGAACA TTGACACATC CCATGGTGAT 
GTGGTCTTCG CTGGCAATGG ACAGGAGGGT ACACCGGGGA AGGACTTTGC ACTTCTCACA 
CAGTGTAACC ACACCATCAT GACTATTGGC ACCTTTGGCT TCTGGGCTGC CTACTTAGCT 
GGTGGAGACA CGGTCTACCT TGCAAACTTC ACCCTGCCAG ATTCGGAGTT TCTGAAGATC 
TTCAGGCCTG AGGCTGCCTT CCTGCCTGAG TGGGTGGGCA TCAATGCAGA CTTGTCCCCG 
CTGCAGGCTC AATTTGACCC CTGGAAGCCA GACAGTCTTT TTAGATTGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540679.1
CDS	232..1365
Translation	MWTPSRRQLCLAFLLVCVLSAGSFFFHLNGGNFFRNGLTLSVLCSDYHLLKSPVAMVCLPHPLQTSNGSPSCPEQSSSLSGTWTITPGGRFGNQMGQYATLLALAQLNGRQAFIQPEMHAALAPVFRISLPVLDPEVDSLTPWQHLVLHDWMSEEYSHLEDPFLKLSGFPCSWTFFHHLREQIRREFTLHNHLREGAQYLLSGLRIGPAGIRPHTFVGVHVRRGDYLEVMPNRWKGVVGDRAYLQQAMDWFRARHKDPIFVVTSNGMKWCLENIDTSHGDVVFAGNGQEGTPGKDFALLTQCNHTIMTIGTFGFWAAYLAGGDTVYLANFTLPDSEFLKIFRPEAAFLPEWVGINADLSPLQAQFDPWKPDSLFRLV

Target ORF information:

RefSeq Version	XM_006540616.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus fucosyltransferase 1 (Fut1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540616.3

ORF Insert Sequence:

ATGTGGACTC CCAGCCGGAG GCAGCTCTGC CTGGCATTTC TGTTGGTCTG TGTGCTCTCT 
GCCGGCTCCT TCTTTTTCCA CCTGAATGGA GGAAACTTCT TTCGAAATGG TCTAACTCTC 
TCTGTCCTGT GTTCAGACTA TCACCTGCTG AAGTCCCCAG TGGCCATGGT ATGCCTACCT 
CATCCATTGC AGACATCTAA TGGCTCCCCT TCCTGTCCTG AGCAGTCCTC CTCACTCTCT 
GGGACTTGGA CAATCACCCC AGGAGGCAGG TTTGGTAACC AGATGGGGCA GTATGCTACA 
TTGCTGGCCC TAGCCCAGCT CAATGGTCGC CAAGCCTTCA TCCAACCTGA GATGCATGCC 
GCCCTGGCCC CCGTGTTCCG AATCTCCCTG CCAGTGCTGG ACCCTGAGGT GGACAGCCTC 
ACACCTTGGC AGCACTTAGT CCTACATGAC TGGATGTCAG AGGAGTACTC CCATCTGGAG 
GACCCATTTC TCAAGCTGTC TGGTTTCCCC TGCTCTTGGA CCTTTTTCCA TCATCTTCGG 
GAACAGATTC GTAGGGAATT CACTCTGCAT AACCATCTAC GGGAAGGTGC CCAGTACCTG 
TTGAGCGGGC TCCGTATAGG CCCGGCGGGC ATCCGCCCTC ATACCTTTGT GGGTGTCCAT 
GTGCGTCGTG GAGACTATCT GGAGGTGATG CCCAATCGCT GGAAGGGTGT GGTGGGTGAC 
CGAGCTTACC TCCAGCAAGC CATGGACTGG TTCCGGGCCC GACACAAAGA CCCCATCTTT 
GTGGTCACCA GCAATGGCAT GAAATGGTGT TTGGAGAACA TTGACACATC CCATGGTGAT 
GTGGTCTTCG CTGGCAATGG ACAGGAGGGT ACACCGGGGA AGGACTTTGC ACTTCTCACA 
CAGTGTAACC ACACCATCAT GACTATTGGC ACCTTTGGCT TCTGGGCTGC CTACTTAGCT 
GGTGGAGACA CGGTCTACCT TGCAAACTTC ACCCTGCCAG ATTCGGAGTT TCTGAAGATC 
TTCAGGCCTG AGGCTGCCTT CCTGCCTGAG TGGGTGGGCA TCAATGCAGA CTTGTCCCCG 
CTGCAGGCTC AATTTGACCC CTGGAAGCCA GACAGTCTTT TTAGATTGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

miR-339-5p downregulation contributes to Taxol resistance in small-cell lung cancer by targeting ?1,2-fucosyltransferase 1.
IUBMB life69(11)841-849(2017 Nov)
Gan CZ,Li G,Luo QS,Li HM

A key role for Fut1-regulated angiogenesis and ICAM-1 expression in K/BxN arthritis.
Annals of the rheumatic diseases74(7)1459-66(2015 Jul)
Amin MA,Campbell PL,Ruth JH,Isozaki T,Rabquer BJ,Alex Stinson W,O'Brien M,Edhayan G,Ohara RA,Vargo J,Domino SE,Koch AE

Different effects of androgen on the expression of Fut1, Fut2, Fut4 and Fut9 in male mouse reproductive tract.
International journal of molecular sciences14(11)23188-202(2013 Nov)
Wang CM,Hu SG,Ru YF,Yao GX,Ma WB,Gu YH,Chu C,Wang SL,Zhou ZM,Liu Q,Zhou YC,Zhang YL

A phenotype survey of 36 mutant mouse strains with gene-targeted defects in glycosyltransferases or glycan-binding proteins.
Glycobiology23(3)363-80(2013 Mar)
Orr SL,Le D,Long JM,Sobieszczuk P,Ma B,Tian H,Fang X,Paulson JC,Marth JD,Varki N

Distinct fucosylation of M cells and epithelial cells by Fut1 and Fut2, respectively, in response to intestinal environmental stress.
Biochemical and biophysical research communications404(3)822-8(2011 Jan)
Terahara K,Nochi T,Yoshida M,Takahashi Y,Goto Y,Hatai H,Kurokawa S,Jang MH,Kweon MN,Domino SE,Hiroi T,Yuki Y,Tsunetsugu-Yokota Y,Kobayashi K,Kiyono H

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Fut1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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