Fst cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fst
Entrez Gene ID	14313
Full Name	follistatin
Synonyms	AL033346,FS
General protein information	Preferred Names follistatin Names follistatin activin-binding protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13\|13 D2.2
Summary	The protein encoded by this gene binds to and negatively regulates activin, as well as other members of the transforming growth factor beta family, and acts to prevent uncontrolled cellular proliferation. This protein also contains a heparin-binding sequence. It is expressed in many of the tissues in which activin is synthesized and is thought to clear activin from the circulation by attachment to the cell surface. Alternative splicing results in multiple transcript variants that encode multiple protein isoforms, including FST315 and FST288, that differ at their C-terminus. Another isoform, FST303 is thought to be produced by proteolytic cleavage of FST315. These isoforms differ in their localization and in their ability to bind heparin. While FST315 is a circulating protein, FST288 is tissue-bound, and FST303 is gonad-specific. While deletion of all isoforms results in embryonic lethality, expression of just FST288 is sufficient for embryonic development, but the resultant mice have fertility defects. [provided by RefSeq, Aug 2014].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001301375.1	NP_001288304.1	follistatin FST288 precursor
NM_008046.3	NP_032072.1	follistatin isoform 2 precursor
XM_030247150.1	XP_030103010.1	follistatin isoform X1
NM_001301373.1	NP_001288302.1	follistatin FST315 precursor

Pathways from BioSystems

Homologies

	FST	XP_001095829.2
Mus musculus (house mouse)	Fst	NP_032072.1
Xenopus tropicalis (tropical clawed frog)	fst	NP_001008057.1
Canis lupus familiaris (dog)	FST	XP_005619514.1
Bos taurus (cattle)	FST	NP_786995.2
Rattus norvegicus (Norway rat)	Fst	NP_036693.1
Gallus gallus (chicken)	FST	NP_990531.1
Pan troglodytes (chimpanzee)	FST	XP_003310757.1
Danio rerio (zebrafish)	fstb	NP_001034720.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006282	XP_316346.4
Homo sapiens (human)	FST	NP_037541.1
Danio rerio (zebrafish)	fsta	NP_571112.3

Gene Ontology
Bibliography

Related articles in PubMed

The follistatin-288 isoform alone is sufficient for survival but not for normal fertility in mice.
Kimura F, Sidis Y, Bonomi L, Xia Y, Schneyer A
Endocrinology151(3)1310-9(2010 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Hypergravity affects follistatin levels in muscle through the vestibular system in mice. Follistatin may play some roles in the interactions between muscle and bone metabolism in response to gravity change.
Title: Role of follistatin in muscle and bone alterations induced by gravity change in mice.

Fst promotes brown adipocyte characteristics in both white adipose tissue and brown adipose tissue depots in vivo through distinct mechanisms.
Title: Follistatin Targets Distinct Pathways To Promote Brown Adipocyte Characteristics in Brown and White Adipose Tissues.

Results showed activin-C and follistatin are differentially expressed during prostate development and suggested that the antagonistic property of follistatin is secondary to the action of activin-C. Study provides evidence to support a role of activin-C in prostate development and provides new insights in the spatiotemporal localization of activins and their antagonists during mouse prostate development.
Title: Regional localization of activin-?

OVOL1-regulated Fst and SFRP1 affect hair inductive potency of neonatal dermal cells.
Title: Follistatin and secreted frizzled-related protein 1, OVO homolog-like 1-regulated genes, are important for hair follicle neogenesis.

This study assesses the regulation of activin A and follistatin in a murine myocardial ischaemia-reperfusion model.
Title: Regulation and actions of activin A and follistatin in myocardial ischaemia-reperfusion injury.

The following Fst gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fst cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu67770	NM_001301373.1 Latest version!	Mus musculus follistatin (Fst), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OMu22326	NM_008046.3 Latest version!	Mus musculus follistatin (Fst), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu67771	NM_001301375.1 Latest version!	Mus musculus follistatin (Fst), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu86219	XM_030247150.1 Latest version!	Mus musculus follistatin (Fst), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fst ( NM_001301373.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001288302.1 Mus musculus follistatin (Fst), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu67770
Clone ID Related Accession (Same CDS sequence)	NM_001301373.1
Accession Version	NM_001301373.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	follistatin FST315 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK079916.1, BC144926.1, Z29532.1, AC110817.6, AW987053.1 and BX514305.1. On Aug 16, 2014 this sequence version replaced XM_006517527.1. Transcript Variant: This variant (1) encodes the longest isoform, FST315 (PMID:17932109). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC144926.1, X83377.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTCTGCG CCAGGCACCA GCCCGGCGGG CTCTGCCTCC TGCTGCTGCT ACTCTGCCAG 
TTCATGGAGG ACCGCAGCGC CCAGGCTGGG AATTGCTGGC TCCGCCAAGC AAAGAACGGC 
CGCTGCCAGG TCCTGTATAA GACAGAACTG AGCAAGGAAG AGTGTTGCAG CACCGGCCGG 
CTGAGCACCT CATGGACCGA GGAGGATGTG AACGACAATA CTCTCTTCAA GTGGATGATT 
TTCAACGGGG GCGCCCCCAA CTGCATCCCT TGTAAAGAAA CGTGTGAGAA CGTGGACTGC 
GGACCTGGGA AAAAATGTCG AATGAACAAG AAGAATAAAC CCCGCTGCGT CTGTGCCCCA 
GACTGTTCCA ACATCACCTG GAAGGGCCCA GTGTGTGGGC TGGATGGGAA AACCTACCGC 
AACGAATGTG CACTCCTCAA GGCCAGATGC AAAGAGCAGC CGGAACTAGA AGTACAGTAC 
CAGGGCAAAT GTAAAAAGAC TTGTAGGGAT GTTTTTTGTC CAGGCAGCTC CACTTGTGTG 
GTGGATCAGA CCAATAATGC CTACTGTGTG ACCTGTAATC GGATTTGCCC AGAGCCCTCC 
TCTTCTGAAC AGTACCTTTG TGGAAATGAT GGAGTGACTT ACTCCAGCGC CTGCCACCTG 
AGAAAGGCCA CCTGCTTGCT GGGCAGATCC ATTGGATTAG CCTATGAGGG AAAGTGTATC 
AAAGCAAAGT CCTGTGAAGA TATCCAGTGT GGCGGCGGGA AAAAATGCCT ATGGGATTCC 
AAGGTTGGCA GAGGTCGCTG CTCTCTCTGC GATGAGCTGT GTCCTGACAG TAAGTCGGAT 
GAGCCGGTCT GTGCCAGTGA CAATGCCACA TACGCCAGCG AGTGTGCCAT GAAGGAAGCT 
GCCTGCTCTT CTGGCGTGCT TCTTGAAGTG AAGCATTCTG GATCTTGCAA CTCCATCTCG 
GAAGAAACGG AGGAAGAGGA GGAGGAGGAA GACCAGGACT ACAGCTTTCC TATCTCTTCC 
ATTCTAGAGT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288302.1
CDS	366..1400
Translation	MVCARHQPGGLCLLLLLLCQFMEDRSAQAGNCWLRQAKNGRCQVLYKTELSKEECCSTGRLSTSWTEEDVNDNTLFKWMIFNGGAPNCIPCKETCENVDCGPGKKCRMNKKNKPRCVCAPDCSNITWKGPVCGLDGKTYRNECALLKARCKEQPELEVQYQGKCKKTCRDVFCPGSSTCVVDQTNNAYCVTCNRICPEPSSSEQYLCGNDGVTYSSACHLRKATCLLGRSIGLAYEGKCIKAKSCEDIQCGGGKKCLWDSKVGRGRCSLCDELCPDSKSDEPVCASDNATYASECAMKEAACSSGVLLEVKHSGSCNSISEETEEEEEEEDQDYSFPISSILEW

Target ORF information:

RefSeq Version	NM_001301373.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus follistatin (Fst), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301373.1

ORF Insert Sequence:

ATGGTCTGCG CCAGGCACCA GCCCGGCGGG CTCTGCCTCC TGCTGCTGCT ACTCTGCCAG 
TTCATGGAGG ACCGCAGCGC CCAGGCTGGG AATTGCTGGC TCCGCCAAGC AAAGAACGGC 
CGCTGCCAGG TCCTGTATAA GACAGAACTG AGCAAGGAAG AGTGTTGCAG CACCGGCCGG 
CTGAGCACCT CATGGACCGA GGAGGATGTG AACGACAATA CTCTCTTCAA GTGGATGATT 
TTCAACGGGG GCGCCCCCAA CTGCATCCCT TGTAAAGAAA CGTGTGAGAA CGTGGACTGC 
GGACCTGGGA AAAAATGTCG AATGAACAAG AAGAATAAAC CCCGCTGCGT CTGTGCCCCA 
GACTGTTCCA ACATCACCTG GAAGGGCCCA GTGTGTGGGC TGGATGGGAA AACCTACCGC 
AACGAATGTG CACTCCTCAA GGCCAGATGC AAAGAGCAGC CGGAACTAGA AGTACAGTAC 
CAGGGCAAAT GTAAAAAGAC TTGTAGGGAT GTTTTTTGTC CAGGCAGCTC CACTTGTGTG 
GTGGATCAGA CCAATAATGC CTACTGTGTG ACCTGTAATC GGATTTGCCC AGAGCCCTCC 
TCTTCTGAAC AGTACCTTTG TGGAAATGAT GGAGTGACTT ACTCCAGCGC CTGCCACCTG 
AGAAAGGCCA CCTGCTTGCT GGGCAGATCC ATTGGATTAG CCTATGAGGG AAAGTGTATC 
AAAGCAAAGT CCTGTGAAGA TATCCAGTGT GGCGGCGGGA AAAAATGCCT ATGGGATTCC 
AAGGTTGGCA GAGGTCGCTG CTCTCTCTGC GATGAGCTGT GTCCTGACAG TAAGTCGGAT 
GAGCCGGTCT GTGCCAGTGA CAATGCCACA TACGCCAGCG AGTGTGCCAT GAAGGAAGCT 
GCCTGCTCTT CTGGCGTGCT TCTTGAAGTG AAGCATTCTG GATCTTGCAA CTCCATCTCG 
GAAGAAACGG AGGAAGAGGA GGAGGAGGAA GACCAGGACT ACAGCTTTCC TATCTCTTCC 
ATTCTAGAGT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fst ( NM_008046.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032072.1 Mus musculus follistatin (Fst), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu22326
Clone ID Related Accession (Same CDS sequence)	NM_008046.3
Accession Version	NM_008046.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	follistatin isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK079916.1, BC144926.1, Z29532.1, AC110817.6, AW987053.1 and BX514305.1. On Aug 16, 2014 this sequence version replaced NM_008046.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site compared to variant 1. It encodes isoform 2 which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Z29532.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTCTGCG CCAGGCACCA GCCCGGCGGG CTCTGCCTCC TGCTGCTGCT ACTCTGCCAG 
TTCATGGAGG ACCGCAGCGC CCAGGCTGGG AATTGCTGGC TCCGCCAAGC AAAGAACGGC 
CGCTGCCAGG TCCTGTATAA GACAGAACTG AGCAAGGAAG AGTGTTGCAG CACCGGCCGG 
CTGAGCACCT CATGGACCGA GGAGGATGTG AACGACAATA CTCTCTTCAA GTGGATGATT 
TTCAACGGGG GCGCCCCCAA CTGCATCCCT TGTAAAGAAA CGTGTGAGAA CGTGGACTGC 
GGACCTGGGA AAAAATGTCG AATGAACAAG AAGAATAAAC CCCGCTGCGT CTGTGCCCCA 
GACTGTTCCA ACATCACCTG GAAGGGCCCA GTGTGTGGGC TGGATGGGAA AACCTACCGC 
AACGAATGTG CACTCCTCAA GGCCAGATGC AAAGAGCAGC CGGAACTAGA AGTACAGTAC 
CAGGGCAAAT GTAAAAAGAC TTGTAGGGAT GTTTTTTGTC CAGGCAGCTC CACTTGTGTG 
GTGGATCAGA CCAATAATGC CTACTGTGTG ACCTGTAATC GGATTTGCCC AGAGCCCTCC 
TCTTCTGAAC AGTACCTTTG TGGAAATGAT GGAGTGACTT ACTCCAGCGC CTGCCACCTG 
AGAAAGGCCA CCTGCTTGCT GGGCAGATCC ATTGGATTAG CCTATGAGGG AAAGTGTATC 
ACAAAGTCCT GTGAAGATAT CCAGTGTGGC GGCGGGAAAA AATGCCTATG GGATTCCAAG 
GTTGGCAGAG GTCGCTGCTC TCTCTGCGAT GAGCTGTGTC CTGACAGTAA GTCGGATGAG 
CCGGTCTGTG CCAGTGACAA TGCCACATAC GCCAGCGAGT GTGCCATGAA GGAAGCTGCC 
TGCTCTTCTG GCGTGCTTCT TGAAGTGAAG CATTCTGGAT CTTGCAACTC CATCTCGGAA 
GAAACGGAGG AAGAGGAGGA GGAGGAAGAC CAGGACTACA GCTTTCCTAT CTCTTCCATT 
CTAGAGTGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032072.1
CDS	366..1397
Translation	MVCARHQPGGLCLLLLLLCQFMEDRSAQAGNCWLRQAKNGRCQVLYKTELSKEECCSTGRLSTSWTEEDVNDNTLFKWMIFNGGAPNCIPCKETCENVDCGPGKKCRMNKKNKPRCVCAPDCSNITWKGPVCGLDGKTYRNECALLKARCKEQPELEVQYQGKCKKTCRDVFCPGSSTCVVDQTNNAYCVTCNRICPEPSSSEQYLCGNDGVTYSSACHLRKATCLLGRSIGLAYEGKCITKSCEDIQCGGGKKCLWDSKVGRGRCSLCDELCPDSKSDEPVCASDNATYASECAMKEAACSSGVLLEVKHSGSCNSISEETEEEEEEEDQDYSFPISSILEW

Target ORF information:

RefSeq Version	NM_008046.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus follistatin (Fst), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008046.3

ORF Insert Sequence:

ATGGTCTGCG CCAGGCACCA GCCCGGCGGG CTCTGCCTCC TGCTGCTGCT ACTCTGCCAG 
TTCATGGAGG ACCGCAGCGC CCAGGCTGGG AATTGCTGGC TCCGCCAAGC AAAGAACGGC 
CGCTGCCAGG TCCTGTATAA GACAGAACTG AGCAAGGAAG AGTGTTGCAG CACCGGCCGG 
CTGAGCACCT CATGGACCGA GGAGGATGTG AACGACAATA CTCTCTTCAA GTGGATGATT 
TTCAACGGGG GCGCCCCCAA CTGCATCCCT TGTAAAGAAA CGTGTGAGAA CGTGGACTGC 
GGACCTGGGA AAAAATGTCG AATGAACAAG AAGAATAAAC CCCGCTGCGT CTGTGCCCCA 
GACTGTTCCA ACATCACCTG GAAGGGCCCA GTGTGTGGGC TGGATGGGAA AACCTACCGC 
AACGAATGTG CACTCCTCAA GGCCAGATGC AAAGAGCAGC CGGAACTAGA AGTACAGTAC 
CAGGGCAAAT GTAAAAAGAC TTGTAGGGAT GTTTTTTGTC CAGGCAGCTC CACTTGTGTG 
GTGGATCAGA CCAATAATGC CTACTGTGTG ACCTGTAATC GGATTTGCCC AGAGCCCTCC 
TCTTCTGAAC AGTACCTTTG TGGAAATGAT GGAGTGACTT ACTCCAGCGC CTGCCACCTG 
AGAAAGGCCA CCTGCTTGCT GGGCAGATCC ATTGGATTAG CCTATGAGGG AAAGTGTATC 
ACAAAGTCCT GTGAAGATAT CCAGTGTGGC GGCGGGAAAA AATGCCTATG GGATTCCAAG 
GTTGGCAGAG GTCGCTGCTC TCTCTGCGAT GAGCTGTGTC CTGACAGTAA GTCGGATGAG 
CCGGTCTGTG CCAGTGACAA TGCCACATAC GCCAGCGAGT GTGCCATGAA GGAAGCTGCC 
TGCTCTTCTG GCGTGCTTCT TGAAGTGAAG CATTCTGGAT CTTGCAACTC CATCTCGGAA 
GAAACGGAGG AAGAGGAGGA GGAGGAAGAC CAGGACTACA GCTTTCCTAT CTCTTCCATT 
CTAGAGTGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fst ( NM_001301375.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001288304.1 Mus musculus follistatin (Fst), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu67771
Clone ID Related Accession (Same CDS sequence)	NM_001301375.1
Accession Version	NM_001301375.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	follistatin FST288 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK079916.1, BC144926.1, AK083556.1, AC110817.6, Z29532.1, AW987053.1 and BX514305.1. On Aug 16, 2014 this sequence version replaced XM_006517528.1. Transcript Variant: This variant (3) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. It encodes FST288 (PMID:20032047), which has a shorter C-terminus, compared to FST315. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK083556.1, AK079916.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTCTGCG CCAGGCACCA GCCCGGCGGG CTCTGCCTCC TGCTGCTGCT ACTCTGCCAG 
TTCATGGAGG ACCGCAGCGC CCAGGCTGGG AATTGCTGGC TCCGCCAAGC AAAGAACGGC 
CGCTGCCAGG TCCTGTATAA GACAGAACTG AGCAAGGAAG AGTGTTGCAG CACCGGCCGG 
CTGAGCACCT CATGGACCGA GGAGGATGTG AACGACAATA CTCTCTTCAA GTGGATGATT 
TTCAACGGGG GCGCCCCCAA CTGCATCCCT TGTAAAGAAA CGTGTGAGAA CGTGGACTGC 
GGACCTGGGA AAAAATGTCG AATGAACAAG AAGAATAAAC CCCGCTGCGT CTGTGCCCCA 
GACTGTTCCA ACATCACCTG GAAGGGCCCA GTGTGTGGGC TGGATGGGAA AACCTACCGC 
AACGAATGTG CACTCCTCAA GGCCAGATGC AAAGAGCAGC CGGAACTAGA AGTACAGTAC 
CAGGGCAAAT GTAAAAAGAC TTGTAGGGAT GTTTTTTGTC CAGGCAGCTC CACTTGTGTG 
GTGGATCAGA CCAATAATGC CTACTGTGTG ACCTGTAATC GGATTTGCCC AGAGCCCTCC 
TCTTCTGAAC AGTACCTTTG TGGAAATGAT GGAGTGACTT ACTCCAGCGC CTGCCACCTG 
AGAAAGGCCA CCTGCTTGCT GGGCAGATCC ATTGGATTAG CCTATGAGGG AAAGTGTATC 
AAAGCAAAGT CCTGTGAAGA TATCCAGTGT GGCGGCGGGA AAAAATGCCT ATGGGATTCC 
AAGGTTGGCA GAGGTCGCTG CTCTCTCTGC GATGAGCTGT GTCCTGACAG TAAGTCGGAT 
GAGCCGGTCT GTGCCAGTGA CAATGCCACA TACGCCAGCG AGTGTGCCAT GAAGGAAGCT 
GCCTGCTCTT CTGGCGTGCT TCTTGAAGTG AAGCATTCTG GATCTTGCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288304.1
CDS	366..1319
Translation	MVCARHQPGGLCLLLLLLCQFMEDRSAQAGNCWLRQAKNGRCQVLYKTELSKEECCSTGRLSTSWTEEDVNDNTLFKWMIFNGGAPNCIPCKETCENVDCGPGKKCRMNKKNKPRCVCAPDCSNITWKGPVCGLDGKTYRNECALLKARCKEQPELEVQYQGKCKKTCRDVFCPGSSTCVVDQTNNAYCVTCNRICPEPSSSEQYLCGNDGVTYSSACHLRKATCLLGRSIGLAYEGKCIKAKSCEDIQCGGGKKCLWDSKVGRGRCSLCDELCPDSKSDEPVCASDNATYASECAMKEAACSSGVLLEVKHSGSCN

Target ORF information:

RefSeq Version	NM_001301375.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus follistatin (Fst), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301375.1

ORF Insert Sequence:

ATGGTCTGCG CCAGGCACCA GCCCGGCGGG CTCTGCCTCC TGCTGCTGCT ACTCTGCCAG 
TTCATGGAGG ACCGCAGCGC CCAGGCTGGG AATTGCTGGC TCCGCCAAGC AAAGAACGGC 
CGCTGCCAGG TCCTGTATAA GACAGAACTG AGCAAGGAAG AGTGTTGCAG CACCGGCCGG 
CTGAGCACCT CATGGACCGA GGAGGATGTG AACGACAATA CTCTCTTCAA GTGGATGATT 
TTCAACGGGG GCGCCCCCAA CTGCATCCCT TGTAAAGAAA CGTGTGAGAA CGTGGACTGC 
GGACCTGGGA AAAAATGTCG AATGAACAAG AAGAATAAAC CCCGCTGCGT CTGTGCCCCA 
GACTGTTCCA ACATCACCTG GAAGGGCCCA GTGTGTGGGC TGGATGGGAA AACCTACCGC 
AACGAATGTG CACTCCTCAA GGCCAGATGC AAAGAGCAGC CGGAACTAGA AGTACAGTAC 
CAGGGCAAAT GTAAAAAGAC TTGTAGGGAT GTTTTTTGTC CAGGCAGCTC CACTTGTGTG 
GTGGATCAGA CCAATAATGC CTACTGTGTG ACCTGTAATC GGATTTGCCC AGAGCCCTCC 
TCTTCTGAAC AGTACCTTTG TGGAAATGAT GGAGTGACTT ACTCCAGCGC CTGCCACCTG 
AGAAAGGCCA CCTGCTTGCT GGGCAGATCC ATTGGATTAG CCTATGAGGG AAAGTGTATC 
AAAGCAAAGT CCTGTGAAGA TATCCAGTGT GGCGGCGGGA AAAAATGCCT ATGGGATTCC 
AAGGTTGGCA GAGGTCGCTG CTCTCTCTGC GATGAGCTGT GTCCTGACAG TAAGTCGGAT 
GAGCCGGTCT GTGCCAGTGA CAATGCCACA TACGCCAGCG AGTGTGCCAT GAAGGAAGCT 
GCCTGCTCTT CTGGCGTGCT TCTTGAAGTG AAGCATTCTG GATCTTGCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fst ( XM_030247150.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030103010.1 Mus musculus follistatin (Fst), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu86219
Clone ID Related Accession (Same CDS sequence)	XM_030247150.1
Accession Version	XM_030247150.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	follistatin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTGCG CCAGGCACCA GCCCGGCGGG CTCTGCCTCC TGCTGCTGCT ACTCTGCCAG 
TTCATGGAGG ACCGCAGCGC CCAGGCTGGG AATTGCTGGC TCCGCCAAGC AAAGAACGGC 
CGCTGCCAGG TCCTGTATAA GACAGAACTG AGCAAGGAAG AGTGTTGCAG CACCGGCCGG 
CTGAGCACCT CATGGACCGA GGAGGATGTG AACGACAATA CTCTCTTCAA GTGGATGATT 
TTCAACGGGG GCGCCCCCAA CTGCATCCCT TGTAAAGAAA CGTGTGAGAA CGTGGACTGC 
GGACCTGGGA AAAAATGTCG AATGAACAAG AAGAATAAAC CCCGCTGCGT CTGTGCCCCA 
GACTGTTCCA ACATCACCTG GAAGGGCCCA GTGTGTGGGC TGGATGGGAA AACCTACCGC 
AACGAATGTG CACTCCTCAA GGCCAGATGC AAAGAGCAGC CGGAACTAGA AGTACAGTAC 
CAGGGCAAAT GTAAAAAGAC TTGTAGGGAT GTTTTTTGTC CAGGCAGCTC CACTTGTGTG 
GTGGATCAGA CCAATAATGC CTACTGTGTG ACCTGTAATC GGATTTGCCC AGAGCCCTCC 
TCTTCTGAAC AGTACCTTTG TGGAAATGAT GGAGTGACTT ACTCCAGCGC CTGCCACCTG 
AGAAAGGCCA CCTGCTTGCT GGGCAGATCC ATTGGATTAG CCTATGAGGG AAAGTGTATC 
ACAAAGTCCT GTGAAGATAT CCAGTGTGGC GGCGGGAAAA AATGCCTATG GGATTCCAAG 
GTTGGCAGAG GTCGCTGCTC TCTCTGCGAT GAGCTGTGTC CTGACAGTAA GTCGGATGAG 
CCGGTCTGTG CCAGTGACAA TGCCACATAC GCCAGCGAGT GTGCCATGAA GGAAGCTGCC 
TGCTCTTCTG GCGTGCTTCT TGAAGTGAAG CATTCTGGAT CTTGCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030103010.1
CDS	404..1354
Translation	MVCARHQPGGLCLLLLLLCQFMEDRSAQAGNCWLRQAKNGRCQVLYKTELSKEECCSTGRLSTSWTEEDVNDNTLFKWMIFNGGAPNCIPCKETCENVDCGPGKKCRMNKKNKPRCVCAPDCSNITWKGPVCGLDGKTYRNECALLKARCKEQPELEVQYQGKCKKTCRDVFCPGSSTCVVDQTNNAYCVTCNRICPEPSSSEQYLCGNDGVTYSSACHLRKATCLLGRSIGLAYEGKCITKSCEDIQCGGGKKCLWDSKVGRGRCSLCDELCPDSKSDEPVCASDNATYASECAMKEAACSSGVLLEVKHSGSCN

Target ORF information:

RefSeq Version	XM_030247150.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus follistatin (Fst), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030247150.1

ORF Insert Sequence:

ATGGTCTGCG CCAGGCACCA GCCCGGCGGG CTCTGCCTCC TGCTGCTGCT ACTCTGCCAG 
TTCATGGAGG ACCGCAGCGC CCAGGCTGGG AATTGCTGGC TCCGCCAAGC AAAGAACGGC 
CGCTGCCAGG TCCTGTATAA GACAGAACTG AGCAAGGAAG AGTGTTGCAG CACCGGCCGG 
CTGAGCACCT CATGGACCGA GGAGGATGTG AACGACAATA CTCTCTTCAA GTGGATGATT 
TTCAACGGGG GCGCCCCCAA CTGCATCCCT TGTAAAGAAA CGTGTGAGAA CGTGGACTGC 
GGACCTGGGA AAAAATGTCG AATGAACAAG AAGAATAAAC CCCGCTGCGT CTGTGCCCCA 
GACTGTTCCA ACATCACCTG GAAGGGCCCA GTGTGTGGGC TGGATGGGAA AACCTACCGC 
AACGAATGTG CACTCCTCAA GGCCAGATGC AAAGAGCAGC CGGAACTAGA AGTACAGTAC 
CAGGGCAAAT GTAAAAAGAC TTGTAGGGAT GTTTTTTGTC CAGGCAGCTC CACTTGTGTG 
GTGGATCAGA CCAATAATGC CTACTGTGTG ACCTGTAATC GGATTTGCCC AGAGCCCTCC 
TCTTCTGAAC AGTACCTTTG TGGAAATGAT GGAGTGACTT ACTCCAGCGC CTGCCACCTG 
AGAAAGGCCA CCTGCTTGCT GGGCAGATCC ATTGGATTAG CCTATGAGGG AAAGTGTATC 
ACAAAGTCCT GTGAAGATAT CCAGTGTGGC GGCGGGAAAA AATGCCTATG GGATTCCAAG 
GTTGGCAGAG GTCGCTGCTC TCTCTGCGAT GAGCTGTGTC CTGACAGTAA GTCGGATGAG 
CCGGTCTGTG CCAGTGACAA TGCCACATAC GCCAGCGAGT GTGCCATGAA GGAAGCTGCC 
TGCTCTTCTG GCGTGCTTCT TGAAGTGAAG CATTCTGGAT CTTGCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The follistatin-288 isoform alone is sufficient for survival but not for normal fertility in mice.
Endocrinology151(3)1310-9(2010 Mar)
Kimura F,Sidis Y,Bonomi L,Xia Y,Schneyer A

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Fst cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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