Smc1b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Smc1b
Entrez Gene ID	140557
Full Name	structural maintenance of chromosomes 1B
Synonyms	SMC-1B,SMC1beta,Smc1l2
General protein information	Preferred Names structural maintenance of chromosomes 1B Names structural maintenance of chromosomes protein 1B SMC protein 1B SMC-1-beta structural maintenace of chromosomes 1B
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15\|15 E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_080470.1	NP_536718.1	structural maintenance of chromosomes protein 1B

Pathways from BioSystems

Homologies

	SMC1B	XP_001109647.1
Mus musculus (house mouse)	Smc1b	NP_536718.1
Xenopus tropicalis (tropical clawed frog)	smc1b	NP_001265278.1
Homo sapiens (human)	SMC1B	NP_683515.3
Pan troglodytes (chimpanzee)	SMC1B	XP_515195.2
Canis lupus familiaris (dog)	SMC1B	XP_538328.2
Gallus gallus (chicken)	SMC1B	XP_416467.2
Danio rerio (zebrafish)	smc1b	XP_001334257.2
Bos taurus (cattle)	SMC1B	XP_002688026.1
Rattus norvegicus (Norway rat)	Smc1b	NP_001123970.1

Gene Ontology
Bibliography

Related articles in PubMed

SMC1? Substitutes for Many Meiotic Functions of SMC1? but Cannot Protect Telomeres from Damage.
Biswas U, Stevense M, Jessberger R
Current biology : CB(2018 Jan)

Smc1? is required for activation of SAC during mouse oocyte meiosis.
Miao Y, Zhou C, Cui Z, Dai X, Zhang M, Lu Y, Xiong B
Cell cycle (Georgetown, Tex.)16(6)536-544(2017 Mar)

BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis.
Liu W, Wang F, Xu Q, Shi J, Zhang X, Lu X, Zhao ZA, Gao Z, Ma H, Duan E, Gao F, Gao S, Yi Z, Li L
Nature communications814182(2017 Jan)

Distinct Roles of Meiosis-Specific Cohesin Complexes in Mammalian Spermatogenesis.
Biswas U, Hempel K, Llano E, Pendas A, Jessberger R
PLoS genetics12(10)e1006389(2016 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a novel function of Smc1beta as a spindle assembly checkpoint participant beyond its role in chromosome cohesion during mouse oocyte meiosis, identified.
Title: Smc1? is required for activation of SAC during mouse oocyte meiosis.

super resolution microscopy revealed synapsis-associated SYCP1 aberrantly deposited between sister chromatids and on single chromatids in Smc1b-/-Rad21L-/- cells.
Title: Distinct Roles of Meiosis-Specific Cohesin Complexes in Mammalian Spermatogenesis.

Data show that structural maintenance of chromosomes protein 1B (Smc1b) is expressed in somatic cells.
Title: SMC1B is present in mammalian somatic cells and interacts with mitotic cohesin proteins.

Together with previous knowledge, three main functions of SMC1b have emerged, which have multiple consequences for spermatocyte biology.
Title: Meiotic cohesin SMC1? provides prophase I centromeric cohesion and is required for multiple synapsis-associated functions.

We report here evidence from the mouse that partial loss of gene function for either Smc1b or Rec8 causes perturbations in the formation of the synaptonemal complex (SC) and affects both synapsis and recombination between homologs during meiotic prophase
Title: Altered cohesin gene dosage affects Mammalian meiotic chromosome structure and behavior.

The following Smc1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Smc1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu19146	NM_080470.1 Latest version!	Mus musculus structural maintenance of chromosomes 1B (Smc1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$734.30 $1049.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Smc1b ( NM_080470.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_536718.1 Mus musculus structural maintenance of chromosomes 1B (Smc1b), mRNA.

Price & Availability↑

CloneID	OMu19146
Clone ID Related Accession (Same CDS sequence)	NM_080470.1
Accession Version	NM_080470.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-20
Organism	Mus musculus(house mouse)
Product	structural maintenance of chromosomes protein 1B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF303827.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF303827.1, BC129800.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849383 [ECO:0000350] ##Evidence-Data-END##

ATGGGGCACC TTGAGCTGCT GCTCGTGGAG AATTTCAAGT CGTGGCGAGG CCGCCAGGTC 
ATCGGCCCTT TCAAGAGGTT CACCTGCATC ATTGGCCCCA ACGGCTCCGG AAAATCTAAT 
GTAATGGATG CACTTAGTTT TGTAATGGGA GAAAAGACAA CTAATTTAAG AGTAAAAAAC 
ATTCAAGAAC TTATTCATGG AGCACATACT GGAAAACCTG TTTCTTCTTC TGCAAGTGTG 
ACAATTATAT ACATAGAGGA CAGTGGAGAA GAGAAAACAT TCACAAGGAT TATCCGAGGG 
GGGTGCTCAG AATATCATTT TGGGGATAAA CCCGTGAGTC GTTCTGTGTA TGTAGCCCAG 
TTGGAAAACA TAGGCATAAT AGTCAAAGCA CAGAACTGTC TAGTTTTTCA GGGAACTGTA 
GAGTCAATAT CCATGAAAAA GCCCAAAGAG AGAACCCAGT TTTTTGAAGA AATCAGTACT 
TCAGGAGAAT TTATAGGAGA ATATGAGGCA AAGAAAAAGA AATTACAAAA AGCTGAAGAA 
GATGCACAAT TTCATTTTAA TGTGAAAAAA AATGTAGCTG CAGAACGGAA GCATGCAAAA 
ATAGAGAAAG AAGAGGCAGA ACATTACCAA AATCTCCTTG AAGAATTGAA AATAAACAAG 
ATACAACTGA TGCTTTTCCA ACTATATTAT AATGAGGAAA AAATCAATGT TTTAAACACT 
GAATTAGAGC AAATGGACGG GAATTTAAGT GTTGTAAAAG ACACTCTTTC TCACCATGAA 
AACATATTTA AAGCTAAGAA AAAGGATTAT GGAATGTTAA CTAGACAACT ACAGCAAACA 
GCAAAAGAAC TGAAATCTGT TGAAGCAATT TTAAATCAGA AGAGGCCTCA GTACATTAAG 
GCTAAAGAAA ACACTTCTCA TCATCTAAAA AAATTAGATT TGTCTAAGAA ATTGATAACA 
GACAATGAAA AACAATGTTC TAAGCAGGAA GATGGCATAC GAGCCTTAGT GGCAGAACTG 
GCTGATTTGG ATAGAGCATG GAAAAGTTTT GAAAAGCAGA TGGAAGAGAA AATCTTACAA 
AAAGGGCGAG ATATTGAATT GGAAAATAGC CAGCTGGATC GTTACAAACT GCTTAAGGAG 
CAAGTCAGAC GGAAGGTTGG TATAATGACA CAACAACTGG AAAAACTGCA GTGGGAACAG 
AAGGCAGAAA AAGAAAGACT TGCATTTGAA AAGAGGAGGC ATGGAGACAC TCAGGGAAAT 
CTAAAACAGA TAAAAGAACA AATAGAAGAG CATAAAAAAC GAATAGAGAA GTTGGAGGAA 
TATACAAAGA CGTGCATGGA TTGCTTGGAA GATAAAAAAC AGCAAGAAGA GGCCCTGAAA 
AAAGAAATTG AAAATACGAA ATCAAGAATG TCTGAAGTTA ATGAAGAATT GAGTCTTATT 
AGAAATGAAT TGCAGAATGC TGGAATTGAT AACCATGAGG GAAAACGTCA GCAGAAAAGA 
GCAGAAGTTC TGGAACACCT TAAAAGACTT TACCCAGATT CTGTGTTTGG AAGACTGCTT 
GATCTGTGTC ATCCTATTCA TAAGAAGTAC CAGCTGGCTG TGACTAAGCT TTTTGGCCGG 
TACATGGTTG CCATTGTTGT AGCCTCAGAA AAGATAGCGA AAGATTGTAT TCGATTTCTG 
AAGGCAGAAA GAGCTGAACC TGAGACATTC CTTGCTCTAG ATTACCTTGA TATCAAGCCA 
ATCAATGAAC GACTAAGGGA AATTAAAGGC TGTAAGATGA TGATCGATGT TATAAAGACC 
CAGTTTCCTC AGCTGAAGAA AGTGATTCAG TTTGTTTGTG GAAATGGCCT TGTCTGTGAG 
ACTGTGGAAG AAGCAAGACA TATTGCATTC GGTGGACCTG AAAGACGGAA GGCAGTAGCA 
CTTGATGGAA CACTGTTTTT GAAATCTGGA GTGATTTCTG GAGGGTCAAG TGACTTAAAG 
CACAAAGCTC TGTGCTGGGA TGAGAAAGAG TTACACAATC TAAGAGACAA AAGAAGCCAA 
CTAGTCCAAG AGCTAAAGGA GTTAATGAAG ACACTCCGCA AGGAAACAGA TCTGAAGCAA 
ATACAGACTT TAGTACAAGG AACCAATACA CGACTCAAAT ATTCACAAAA TGAACTAGAG 
ATGATTAAAA AGAAGCACCT TGCTACATTT TACCGGGAAC AATCTCAGCT ACAAAGTGAA 
TTACTGAATA TTGATTCTCA ATGTACTATG TTGAGTGAAG GAATCAACAA ACAGCAACAA 
AAAATTGAAG AATTTCAAGA TAAGATAGAT GAGGTAGAAG ATGACATATT CCAAGACTTC 
TGTGAAGAAA TTGGTGTGGA AAATATCCGT GAATTTGAGA ATAAACATGT TAAACAGCAG 
CAAGAAAATG ATCAAAAAAG ATTAGAGTTT GAAAAACAAA AAACTCGGCT TAATATTCAG 
CTCGAATATA GTCGAAATCA GCTTAAGAAG AAACTGAATA ATATCGACAC ATTAAAAACC 
ACAATCCAGA AGGGCAAAGA AGATATTGAT AACCTAAAAA AGACCGAAGA AGAATGTCTG 
AAAATTGTTG AGGAACTCAT GGTGAAGCAA GAGCAAATTA AGGAAGTGCT TGCCACACAG 
AGTTCCAACA TTGAAAAAAT TCACATACAA ATTGAAGAGG AACGCAAGAA GGTTTTGGCT 
GTTGATAGGG AAGTTGGAAA ATTACAGAAG GAAGTTGTAA TCATTCAGGG TTCTTTGGAA 
CAGAAACTGC TAGAGAAACA TAACTTGCTG CTAGATTGCA AAGTTCAAGA CATTGACATA 
AGTCTTGTGC TGGGGTCATT GGAGGACATC ATTGAAATGG AGCTAACTGA AACAGAAAGC 
ACCCAGGCAA CAGCTGATAT CTATGAGAAA GAAGCATCCA TCCAAATAGA CTACAGCCCT 
CTAAGGGAGG ATTTAAAGGC TCTACAATCA GATAAGGAGG TGGAGGCCCA CCTTACACTC 
CTTCTACAGC AAGTAGCATC CCAAGAAAAC ACTCTACTGA AGACTACAGC TCCAAACTTG 
CGAGCACAGG AGAACTTAAA GACTGTCAGA GACAAGTTTC AAGAATCTGC AGATGTTTTT 
GAGGCCAGCA GAAAGGAAGC CAGAATATGT AGGCAAGAGT TTGAACAGGT GAAAAGACGG 
AGGTACGATG CTTTCAGTCA ATGTTTTGAA CACATCTCAG TCTCAATTGA TCAAATCTAC 
AAGAAGCTCT GCAGGAACAA CAGTGCCCAG GCATTTCTTA GCCCAGAGAA CCCTGAAGAA 
CCTTACTTAG ATGGAATTAG CTACAACTGT GTGGCTCCAG GCAAACGGTT CATGCCCATG 
GACAACCTGT CAGGGGGAGA AAAGTGTGTG GCTGCTCTGG CTCTTCTGTT TGCTGTACAC 
AGTTTTCGGC CTGCTCCATT CTTTGTATTA GATGAAGTAG ATGCAGCCCT GGACAATACT 
AACATTGGCA AAGTCTCAAG TTACATCAAA GAGCAGAGTC AGGAACAGTT TCAGATGATA 
ATCATTTCCC TGAAAGAGGA GTTCTACTCC AAAGCTGATG CACTGATAGG CGTCTACCCA 
GAGCACAATG AGTGCATGTT CAGCCATGTG TTGACTCTGG ACCTTTCCAA GTATCCAGAC 
ACCGAAGACC AAGAAGGCAG CAGGAGCCAC CGGAAGCCCA GAGTACCACG AGTATCAATG 
TCTCCAAAGT CTCCCCAGTC TCGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_536718.1
CDS	21..3767
Translation	MGHLELLLVENFKSWRGRQVIGPFKRFTCIIGPNGSGKSNVMDALSFVMGEKTTNLRVKNIQELIHGAHTGKPVSSSASVTIIYIEDSGEEKTFTRIIRGGCSEYHFGDKPVSRSVYVAQLENIGIIVKAQNCLVFQGTVESISMKKPKERTQFFEEISTSGEFIGEYEAKKKKLQKAEEDAQFHFNVKKNVAAERKHAKIEKEEAEHYQNLLEELKINKIQLMLFQLYYNEEKINVLNTELEQMDGNLSVVKDTLSHHENIFKAKKKDYGMLTRQLQQTAKELKSVEAILNQKRPQYIKAKENTSHHLKKLDLSKKLITDNEKQCSKQEDGIRALVAELADLDRAWKSFEKQMEEKILQKGRDIELENSQLDRYKLLKEQVRRKVGIMTQQLEKLQWEQKAEKERLAFEKRRHGDTQGNLKQIKEQIEEHKKRIEKLEEYTKTCMDCLEDKKQQEEALKKEIENTKSRMSEVNEELSLIRNELQNAGIDNHEGKRQQKRAEVLEHLKRLYPDSVFGRLLDLCHPIHKKYQLAVTKLFGRYMVAIVVASEKIAKDCIRFLKAERAEPETFLALDYLDIKPINERLREIKGCKMMIDVIKTQFPQLKKVIQFVCGNGLVCETVEEARHIAFGGPERRKAVALDGTLFLKSGVISGGSSDLKHKALCWDEKELHNLRDKRSQLVQELKELMKTLRKETDLKQIQTLVQGTNTRLKYSQNELEMIKKKHLATFYREQSQLQSELLNIDSQCTMLSEGINKQQQKIEEFQDKIDEVEDDIFQDFCEEIGVENIREFENKHVKQQQENDQKRLEFEKQKTRLNIQLEYSRNQLKKKLNNIDTLKTTIQKGKEDIDNLKKTEEECLKIVEELMVKQEQIKEVLATQSSNIEKIHIQIEEERKKVLAVDREVGKLQKEVVIIQGSLEQKLLEKHNLLLDCKVQDIDISLVLGSLEDIIEMELTETESTQATADIYEKEASIQIDYSPLREDLKALQSDKEVEAHLTLLLQQVASQENTLLKTTAPNLRAQENLKTVRDKFQESADVFEASRKEARICRQEFEQVKRRRYDAFSQCFEHISVSIDQIYKKLCRNNSAQAFLSPENPEEPYLDGISYNCVAPGKRFMPMDNLSGGEKCVAALALLFAVHSFRPAPFFVLDEVDAALDNTNIGKVSSYIKEQSQEQFQMIIISLKEEFYSKADALIGVYPEHNECMFSHVLTLDLSKYPDTEDQEGSRSHRKPRVPRVSMSPKSPQSR

Target ORF information:

RefSeq Version	NM_080470.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus structural maintenance of chromosomes 1B (Smc1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080470.1

ORF Insert Sequence:

ATGGGGCACC TTGAGCTGCT GCTCGTGGAG AATTTCAAGT CGTGGCGAGG CCGCCAGGTC 
ATCGGCCCTT TCAAGAGGTT CACCTGCATC ATTGGCCCCA ACGGCTCCGG AAAATCTAAT 
GTAATGGATG CACTTAGTTT TGTAATGGGA GAAAAGACAA CTAATTTAAG AGTAAAAAAC 
ATTCAAGAAC TTATTCATGG AGCACATACT GGAAAACCTG TTTCTTCTTC TGCAAGTGTG 
ACAATTATAT ACATAGAGGA CAGTGGAGAA GAGAAAACAT TCACAAGGAT TATCCGAGGG 
GGGTGCTCAG AATATCATTT TGGGGATAAA CCCGTGAGTC GTTCTGTGTA TGTAGCCCAG 
TTGGAAAACA TAGGCATAAT AGTCAAAGCA CAGAACTGTC TAGTTTTTCA GGGAACTGTA 
GAGTCAATAT CCATGAAAAA GCCCAAAGAG AGAACCCAGT TTTTTGAAGA AATCAGTACT 
TCAGGAGAAT TTATAGGAGA ATATGAGGCA AAGAAAAAGA AATTACAAAA AGCTGAAGAA 
GATGCACAAT TTCATTTTAA TGTGAAAAAA AATGTAGCTG CAGAACGGAA GCATGCAAAA 
ATAGAGAAAG AAGAGGCAGA ACATTACCAA AATCTCCTTG AAGAATTGAA AATAAACAAG 
ATACAACTGA TGCTTTTCCA ACTATATTAT AATGAGGAAA AAATCAATGT TTTAAACACT 
GAATTAGAGC AAATGGACGG GAATTTAAGT GTTGTAAAAG ACACTCTTTC TCACCATGAA 
AACATATTTA AAGCTAAGAA AAAGGATTAT GGAATGTTAA CTAGACAACT ACAGCAAACA 
GCAAAAGAAC TGAAATCTGT TGAAGCAATT TTAAATCAGA AGAGGCCTCA GTACATTAAG 
GCTAAAGAAA ACACTTCTCA TCATCTAAAA AAATTAGATT TGTCTAAGAA ATTGATAACA 
GACAATGAAA AACAATGTTC TAAGCAGGAA GATGGCATAC GAGCCTTAGT GGCAGAACTG 
GCTGATTTGG ATAGAGCATG GAAAAGTTTT GAAAAGCAGA TGGAAGAGAA AATCTTACAA 
AAAGGGCGAG ATATTGAATT GGAAAATAGC CAGCTGGATC GTTACAAACT GCTTAAGGAG 
CAAGTCAGAC GGAAGGTTGG TATAATGACA CAACAACTGG AAAAACTGCA GTGGGAACAG 
AAGGCAGAAA AAGAAAGACT TGCATTTGAA AAGAGGAGGC ATGGAGACAC TCAGGGAAAT 
CTAAAACAGA TAAAAGAACA AATAGAAGAG CATAAAAAAC GAATAGAGAA GTTGGAGGAA 
TATACAAAGA CGTGCATGGA TTGCTTGGAA GATAAAAAAC AGCAAGAAGA GGCCCTGAAA 
AAAGAAATTG AAAATACGAA ATCAAGAATG TCTGAAGTTA ATGAAGAATT GAGTCTTATT 
AGAAATGAAT TGCAGAATGC TGGAATTGAT AACCATGAGG GAAAACGTCA GCAGAAAAGA 
GCAGAAGTTC TGGAACACCT TAAAAGACTT TACCCAGATT CTGTGTTTGG AAGACTGCTT 
GATCTGTGTC ATCCTATTCA TAAGAAGTAC CAGCTGGCTG TGACTAAGCT TTTTGGCCGG 
TACATGGTTG CCATTGTTGT AGCCTCAGAA AAGATAGCGA AAGATTGTAT TCGATTTCTG 
AAGGCAGAAA GAGCTGAACC TGAGACATTC CTTGCTCTAG ATTACCTTGA TATCAAGCCA 
ATCAATGAAC GACTAAGGGA AATTAAAGGC TGTAAGATGA TGATCGATGT TATAAAGACC 
CAGTTTCCTC AGCTGAAGAA AGTGATTCAG TTTGTTTGTG GAAATGGCCT TGTCTGTGAG 
ACTGTGGAAG AAGCAAGACA TATTGCATTC GGTGGACCTG AAAGACGGAA GGCAGTAGCA 
CTTGATGGAA CACTGTTTTT GAAATCTGGA GTGATTTCTG GAGGGTCAAG TGACTTAAAG 
CACAAAGCTC TGTGCTGGGA TGAGAAAGAG TTACACAATC TAAGAGACAA AAGAAGCCAA 
CTAGTCCAAG AGCTAAAGGA GTTAATGAAG ACACTCCGCA AGGAAACAGA TCTGAAGCAA 
ATACAGACTT TAGTACAAGG AACCAATACA CGACTCAAAT ATTCACAAAA TGAACTAGAG 
ATGATTAAAA AGAAGCACCT TGCTACATTT TACCGGGAAC AATCTCAGCT ACAAAGTGAA 
TTACTGAATA TTGATTCTCA ATGTACTATG TTGAGTGAAG GAATCAACAA ACAGCAACAA 
AAAATTGAAG AATTTCAAGA TAAGATAGAT GAGGTAGAAG ATGACATATT CCAAGACTTC 
TGTGAAGAAA TTGGTGTGGA AAATATCCGT GAATTTGAGA ATAAACATGT TAAACAGCAG 
CAAGAAAATG ATCAAAAAAG ATTAGAGTTT GAAAAACAAA AAACTCGGCT TAATATTCAG 
CTCGAATATA GTCGAAATCA GCTTAAGAAG AAACTGAATA ATATCGACAC ATTAAAAACC 
ACAATCCAGA AGGGCAAAGA AGATATTGAT AACCTAAAAA AGACCGAAGA AGAATGTCTG 
AAAATTGTTG AGGAACTCAT GGTGAAGCAA GAGCAAATTA AGGAAGTGCT TGCCACACAG 
AGTTCCAACA TTGAAAAAAT TCACATACAA ATTGAAGAGG AACGCAAGAA GGTTTTGGCT 
GTTGATAGGG AAGTTGGAAA ATTACAGAAG GAAGTTGTAA TCATTCAGGG TTCTTTGGAA 
CAGAAACTGC TAGAGAAACA TAACTTGCTG CTAGATTGCA AAGTTCAAGA CATTGACATA 
AGTCTTGTGC TGGGGTCATT GGAGGACATC ATTGAAATGG AGCTAACTGA AACAGAAAGC 
ACCCAGGCAA CAGCTGATAT CTATGAGAAA GAAGCATCCA TCCAAATAGA CTACAGCCCT 
CTAAGGGAGG ATTTAAAGGC TCTACAATCA GATAAGGAGG TGGAGGCCCA CCTTACACTC 
CTTCTACAGC AAGTAGCATC CCAAGAAAAC ACTCTACTGA AGACTACAGC TCCAAACTTG 
CGAGCACAGG AGAACTTAAA GACTGTCAGA GACAAGTTTC AAGAATCTGC AGATGTTTTT 
GAGGCCAGCA GAAAGGAAGC CAGAATATGT AGGCAAGAGT TTGAACAGGT GAAAAGACGG 
AGGTACGATG CTTTCAGTCA ATGTTTTGAA CACATCTCAG TCTCAATTGA TCAAATCTAC 
AAGAAGCTCT GCAGGAACAA CAGTGCCCAG GCATTTCTTA GCCCAGAGAA CCCTGAAGAA 
CCTTACTTAG ATGGAATTAG CTACAACTGT GTGGCTCCAG GCAAACGGTT CATGCCCATG 
GACAACCTGT CAGGGGGAGA AAAGTGTGTG GCTGCTCTGG CTCTTCTGTT TGCTGTACAC 
AGTTTTCGGC CTGCTCCATT CTTTGTATTA GATGAAGTAG ATGCAGCCCT GGACAATACT 
AACATTGGCA AAGTCTCAAG TTACATCAAA GAGCAGAGTC AGGAACAGTT TCAGATGATA 
ATCATTTCCC TGAAAGAGGA GTTCTACTCC AAAGCTGATG CACTGATAGG CGTCTACCCA 
GAGCACAATG AGTGCATGTT CAGCCATGTG TTGACTCTGG ACCTTTCCAA GTATCCAGAC 
ACCGAAGACC AAGAAGGCAG CAGGAGCCAC CGGAAGCCCA GAGTACCACG AGTATCAATG 
TCTCCAAAGT CTCCCCAGTC TCGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SMC1? Substitutes for Many Meiotic Functions of SMC1? but Cannot Protect Telomeres from Damage.
Current biology : CB(2018 Jan)
Biswas U,Stevense M,Jessberger R

Smc1? is required for activation of SAC during mouse oocyte meiosis.
Cell cycle (Georgetown, Tex.)16(6)536-544(2017 Mar)
Miao Y,Zhou C,Cui Z,Dai X,Zhang M,Lu Y,Xiong B

BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis.
Nature communications814182(2017 Jan)
Liu W,Wang F,Xu Q,Shi J,Zhang X,Lu X,Zhao ZA,Gao Z,Ma H,Duan E,Gao F,Gao S,Yi Z,Li L

Distinct Roles of Meiosis-Specific Cohesin Complexes in Mammalian Spermatogenesis.
PLoS genetics12(10)e1006389(2016 Oct)
Biswas U,Hempel K,Llano E,Pendas A,Jessberger R

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Smc1b cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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