SMC1B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SMC1B
Entrez Gene ID	27127
Full Name	structural maintenance of chromosomes 1B
Synonyms	SMC1BETA,SMC1L2
General protein information	Preferred Names structural maintenance of chromosomes 1B Names structural maintenance of chromosomes protein 1B SMC protein 1B SMC1 (structural maintenance of chromosomes 1, yeast)-like 1 SMC1 structural maintenance of chromosomes 1-like 2 mitosis-specific chromosome segregation protein like protein beta
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q13.31
Summary	SMC1L2 belongs to a family of proteins required for chromatid cohesion and DNA recombination during meiosis and mitosis (3:Revenkova et al., 2001 [PubMed 11564881]).[supplied by OMIM, Mar 2008].
Disorder MIM:	608685

mRNA and Protein(s)

mRNA	Protein	Name
NM_148674.4	NP_683515.4	structural maintenance of chromosomes protein 1B isoform 1
XM_011530145.2	XP_011528447.1	structural maintenance of chromosomes protein 1B isoform X2
NM_148674.5	NP_683515.4	structural maintenance of chromosomes protein 1B isoform 1
NM_001291501.1	NP_001278430.1	structural maintenance of chromosomes protein 1B isoform 2
NM_001291501.2	NP_001278430.1	structural maintenance of chromosomes protein 1B isoform 2
XM_011530144.2	XP_011528446.1	structural maintenance of chromosomes protein 1B isoform X1

Pathways from BioSystems

Homologies

	SMC1B	XP_001109647.1
Mus musculus (house mouse)	Smc1b	NP_536718.1
Xenopus tropicalis (tropical clawed frog)	smc1b	NP_001265278.1
Homo sapiens (human)	SMC1B	NP_683515.3
Pan troglodytes (chimpanzee)	SMC1B	XP_515195.2
Canis lupus familiaris (dog)	SMC1B	XP_538328.2
Gallus gallus (chicken)	SMC1B	XP_416467.2
Danio rerio (zebrafish)	smc1b	XP_001334257.2
Bos taurus (cattle)	SMC1B	XP_002688026.1
Rattus norvegicus (Norway rat)	Smc1b	NP_001123970.1

Gene Ontology
Bibliography

Related articles in PubMed

RNA immunoprecipitation identifies novel targets of DAZL in human foetal ovary.
Rosario R, Smith RW, Adams IR, Anderson RA
Molecular human reproduction23(3)177-186(2017 Mar)

SMC1B is present in mammalian somatic cells and interacts with mitotic cohesin proteins.
Mannini L, Cucco F, Quarantotti V, Amato C, Tinti M, Tana L, Frattini A, Delia D, Krantz ID, Jessberger R, Musio A
Scientific reports518472(2015 Dec)

Protein deep sequencing applied to biobank samples from patients with pancreatic cancer.
Ansari D, Andersson R, Bauden MP, Andersson B, Connolly JB, Welinder C, Sasor A, Marko-Varga G
Journal of cancer research and clinical oncology141(2)369-80(2015 Feb)

RAD21L, a novel cohesin subunit implicated in linking homologous chromosomes in mammalian meiosis.
Lee J, Hirano T
The Journal of cell biology192(2)263-76(2011 Jan)

The evolution of SMC proteins: phylogenetic analysis and structural implications.
Cobbe N, Heck MM
Molecular biology and evolution21(2)332-47(2004 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Through the translational regulation of novel RNA targets SMC1B and TEX11, DAZL may have a key role in regulating chromosome cohesion and DNA recombination; two processes fundamental in determining oocyte quality and whose establishment in foetal life may support lifelong fertility.
Title: RNA immunoprecipitation identifies novel targets of DAZL in human foetal ovary.

Data show that structural maintenance of chromosomes protein 1B (SMC1B) is expressed in primary fibroblasts.
Title: SMC1B is present in mammalian somatic cells and interacts with mitotic cohesin proteins.

High SMC1B expression is associated with pancreatic cancer.
Title: Protein deep sequencing applied to biobank samples from patients with pancreatic cancer.

Observational study of gene-disease association and gene-gene interaction. (HuGE Navigator)
Title: MicroRNA-related genetic variations as predictors for risk of second primary tumor and/or recurrence in patients with early-stage head and neck cancer.

The following SMC1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMC1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu16114	NM_148674.5 Latest version!	Homo sapiens structural maintenance of chromosomes 1B (SMC1B), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$874.30 $1249.00
OHu16115	NM_001291501.2 Latest version!	Homo sapiens structural maintenance of chromosomes 1B (SMC1B), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu67073	XM_011530144.2 Latest version!	Homo sapiens structural maintenance of chromosomes 1B (SMC1B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu67074	XM_011530145.2 Latest version!	Homo sapiens structural maintenance of chromosomes 1B (SMC1B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OHu16114	NM_148674.4	Homo sapiens structural maintenance of chromosomes 1B (SMC1B), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$874.30 $1249.00
OHu16115	NM_001291501.1	Homo sapiens structural maintenance of chromosomes 1B (SMC1B), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SMC1B ( NM_148674.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_683515.4 Homo sapiens structural maintenance of chromosomes 1B (SMC1B), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu16114
Clone ID Related Accession (Same CDS sequence)	NM_148674.4 , NM_148674.5
Accession Version	NM_148674.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Homo sapiens(human)
Product	structural maintenance of chromosomes protein 1B isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AJ504806.2, BC126208.1, AK125736.1 and BG194018.1. On Apr 18, 2014 this sequence version replaced NM_148674.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCCACC TGGAGCTGCT GCTTGTGGAA AATTTCAAGT CGTGGCGGGG CCGCCAGGTC 
ATTGGCCCCT TCCGGAGGTT CACCTGCATC ATCGGCCCCA ACGGCTCTGG AAAATCTAAT 
GTAATGGATG CACTTAGTTT TGTAATGGGA GAGAAAATAG CTAATTTAAG AGTGAAAAAT 
ATTCAAGAAC TCATTCATGG AGCACATATT GGAAAACCTA TTTCTTCTTC TGCAAGTGTA 
AAAATTATAT ATGTGGAGGA AAGTGGCGAA GAGAAAACAT TTGCAAGGAT TATCCGAGGG 
GGATGCTCAG AATTTCGCTT TAATGATAAT CTTGTGAGTC GTTCTGTTTA CATTGCAGAG 
TTGGAAAAGA TAGGCATAAT AGTCAAAGCA CAAAATTGTT TGGTTTTTCA GGGAACTGTA 
GAGTCAATTT CAGTGAAGAA ACCCAAAGAA AGGACCCAGT TTTTTGAGGA AATCAGCACT 
TCAGGAGAGC TTATAGGAGA ATATGAAGAA AAGAAAAGAA AGTTACAAAA AGCCGAAGAG 
GATGCACAGT TTAACTTTAA TAAGAAAAAA AATATAGCGG CAGAGCGCAG ACAAGCAAAA 
TTAGAGAAGG AAGAGGCAGA ACGTTACCAG AGTCTCCTTG AAGAACTGAA AATGAACAAG 
ATACAACTGC AGCTTTTTCA ACTATACCAT AATGAGAAAA AGATTCATCT CCTGAACACC 
AAGTTAGAGC ATGTGAATAG GGATTTGAGT GTCAAAAGAG AGTCTTTGTC TCATCATGAA 
AACATAGTTA AAGCCAGGAA AAAGGAACAT GGAATGCTAA CTAGACAACT ACAACAAACA 
GAAAAAGAAT TAAAATCGGT TGAAACCCTT TTAAATCAGA AGAGGCCTCA GTACATTAAA 
GCCAAAGAAA ACACTTCTCA CCACCTTAAG AAATTAGATG TGGCTAAGAA ATCAATAAAG 
GACAGCGAAA AACAATGTTC TAAACAGGAA GATGATATAA AAGCCCTGGA GACAGAGCTG 
GCTGATTTAG ATGCTGCATG GAGAAGTTTT GAAAAGCAGA TTGAGGAAGA AATTTTACAT 
AAAAAGCGAG ACATTGAACT GGAAGCCAGT CAGCTGGATC GTTATAAAGA ACTTAAGGAA 
CAAGTAAGAA AGAAAGTAGC TACAATGACT CAACAACTGG AAAAACTGCA GTGGGAACAG 
AAGACAGATG AAGAAAGACT GGCATTTGAA AAGAGGAGGC ATGGAGAAGT TCAGGGAAAT 
CTAAAACAAA TAAAAGAACA AATAGAAGAT CATAAAAAAC GAATAGAGAA GTTAGAGGAG 
TATACAAAGA CATGCATGGA TTGCTTGAAA GAGAAAAAAC AGCAAGAGGA AACCCTAGTG 
GATGAAATTG AAAAAACAAA ATCAAGAATG TCTGAAGTTA ATGAAGAATT GAATCTTATT 
AGAAGTGAAT TGCAGAATGC TGGGATTGAT ACCCATGAGG GAAAACGTCA GCAAAAGAGA 
GCAGAGGTTC TGGAACACCT TAAAAGACTG TACCCAGATT CTGTGTTTGG AAGACTATTT 
GACCTGTGTC ATCCTATTCA TAAGAAATAC CAGCTGGCTG TTACTAAGGT TTTTGGCCGG 
TTCATCACTG CCATTGTTGT AGCCTCTGAA AAGGTAGCAA AAGATTGTAT TCGATTTCTG 
AAGGAGGAAA GAGCTGAACC TGAGACATTC CTCGCTCTAG ATTACCTTGA TATCAAGCCA 
ATCAATGAAA GACTAAGGGA GCTTAAAGGC TGTAAAATGG TGATTGATGT CATAAAGACT 
CAGTTTCCTC AGCTGAAGAA AGTGATTCAG TTTGTGTGTG GAAATGGTCT TGTTTGTGAG 
ACTATGGAAG AAGCAAGGCA TATTGCACTC AGTGGACCTG AAAGACAGAA AACAGTAGCT 
CTTGATGGAA CATTATTTTT AAAATCTGGA GTGATCTCTG GAGGGTCAAG TGACTTAAAA 
TACAAGGCTA GATGCTGGGA TGAGAAAGAG TTAAAGAATC TAAGAGACAG ACGAAGCCAG 
AAAATCCAAG AGCTAAAGGG TTTAATGAAG ACACTCCGCA AAGAAACAGA TTTGAAACAA 
ATACAGACCC TGATACAGGG AACTCAAACA CGACTCAAAT ATTCACAAAA TGAACTAGAG 
ATGATTAAGA AGAAGCACCT TGTTGCTTTT TACCAGGAAC AATCTCAGTT ACAAAGTGAA 
CTACTAAATA TTGAGTCTCA ATGTATTATG TTGAGTGAAG GAATCAAGGA ACGACAACGA 
AGAATTAAAG AATTTCAAGA AAAGATAGAT AAGGTAGAAG ACGATATCTT CCAACACTTC 
TGTGAAGAAA TTGGCGTGGA AAATATTCGT GAATTTGAGA ACAAACATGT TAAACGGCAA 
CAAGAAATTG ATCAAAAAAG ATTAGAATTT GAAAAACAAA AAACTCGGCT TAATGTTCAA 
CTTGAGTATA GTCGCAGTCA CCTTAAGAAG AAACTGAATA AGATCAACAC ATTAAAAGAA 
ACTATCCAGA AAGGTAGTGA AGATATTGAT CACCTAAAGA AGGCTGAAGA AAACTGTCTG 
CAGACAGTGA ATGAACTCAT GGCAAAGCAG CAGCAACTTA AGGACATACG TGTCACTCAG 
AACTCCAGTG CCGAGAAAGT TCAAACTCAA ATTGAAGAGG AACGGAAGAA GTTTCTGGCT 
GTTGATAGGG AAGTGGGGAA ATTGCAAAAA GAAGTTGTAA GTATTCAAAC TTCTCTGGAA 
CAGAAACGAT TAGAGAAGCA TAACTTGCTG CTTGATTGCA AAGTGCAAGA CATTGAGATA 
ATCCTTTTGT CGGGGTCACT GGATGACATC ATTGAAGTGG AGATGGGAAC TGAAGCAGAA 
AGTACCCAGG CAACAATTGA TATCTATGAA AAAGAAGAAG CCTTTGAAAT AGACTACAGC 
TCTCTAAAAG AGGATTTGAA GGCTCTACAG TCTGATCAAG AAATCGAGGC CCACCTTAGG 
CTCTTATTGC AGCAAGTAGC ATCCCAGGAA GATATCTTAC TGAAAACAGC AGCCCCAAAC 
CTACGAGCAC TGGAGAACTT AAAGACTGTC AGAGACAAGT TTCAAGAGTC CACAGATGCT 
TTTGAGGCCA GCAGAAAGGA AGCCAGACTG TGTAGGCAAG AGTTCGAGCA AGTGAAAAAA 
AGGAGATACG ATCTTTTCAC CCAGTGTTTT GAGCATGTCT CAATCTCAAT TGATCAAATC 
TACAAGAAGC TCTGCAGAAA CAACAGCGCC CAAGCATTTC TTAGCCCAGA GAACCCTGAA 
GAACCTTACT TGGAGGGAAT TAGCTATAAC TGTGTGGCCC CAGGCAAACG GTTTATGCCA 
ATGGACAATT TGTCAGGGGG AGAAAAGTGT GTGGCAGCCT TGGCTCTCCT GTTTGCTGTG 
CACAGTTTTC GTCCTGCCCC ATTCTTTGTT TTAGATGAAG TGGATGCAGC CCTAGACAAT 
ACTAACATAG GCAAAGTGTC AAGTTACATC AAAGAGCAAA CTCAAGACCA GTTTCAGATG 
ATAGTCATCT CCCTAAAAGA AGAGTTCTAT TCCAGAGCCG ACGCGCTGAT CGGCATCTAT 
CCTGAGTACG ATGACTGCAT GTTCAGCCGA GTTTTGACCC TAGATCTTTC TCAGTATCCA 
GACACTGAAG GCCAAGAAAG CAGCAAGAGA CACGGAGAGT CCCGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_683515.4
CDS	53..3760
Translation	MAHLELLLVENFKSWRGRQVIGPFRRFTCIIGPNGSGKSNVMDALSFVMGEKIANLRVKNIQELIHGAHIGKPISSSASVKIIYVEESGEEKTFARIIRGGCSEFRFNDNLVSRSVYIAELEKIGIIVKAQNCLVFQGTVESISVKKPKERTQFFEEISTSGELIGEYEEKKRKLQKAEEDAQFNFNKKKNIAAERRQAKLEKEEAERYQSLLEELKMNKIQLQLFQLYHNEKKIHLLNTKLEHVNRDLSVKRESLSHHENIVKARKKEHGMLTRQLQQTEKELKSVETLLNQKRPQYIKAKENTSHHLKKLDVAKKSIKDSEKQCSKQEDDIKALETELADLDAAWRSFEKQIEEEILHKKRDIELEASQLDRYKELKEQVRKKVATMTQQLEKLQWEQKTDEERLAFEKRRHGEVQGNLKQIKEQIEDHKKRIEKLEEYTKTCMDCLKEKKQQEETLVDEIEKTKSRMSEVNEELNLIRSELQNAGIDTHEGKRQQKRAEVLEHLKRLYPDSVFGRLFDLCHPIHKKYQLAVTKVFGRFITAIVVASEKVAKDCIRFLKEERAEPETFLALDYLDIKPINERLRELKGCKMVIDVIKTQFPQLKKVIQFVCGNGLVCETMEEARHIALSGPERQKTVALDGTLFLKSGVISGGSSDLKYKARCWDEKELKNLRDRRSQKIQELKGLMKTLRKETDLKQIQTLIQGTQTRLKYSQNELEMIKKKHLVAFYQEQSQLQSELLNIESQCIMLSEGIKERQRRIKEFQEKIDKVEDDIFQHFCEEIGVENIREFENKHVKRQQEIDQKRLEFEKQKTRLNVQLEYSRSHLKKKLNKINTLKETIQKGSEDIDHLKKAEENCLQTVNELMAKQQQLKDIRVTQNSSAEKVQTQIEEERKKFLAVDREVGKLQKEVVSIQTSLEQKRLEKHNLLLDCKVQDIEIILLSGSLDDIIEVEMGTEAESTQATIDIYEKEEAFEIDYSSLKEDLKALQSDQEIEAHLRLLLQQVASQEDILLKTAAPNLRALENLKTVRDKFQESTDAFEASRKEARLCRQEFEQVKKRRYDLFTQCFEHVSISIDQIYKKLCRNNSAQAFLSPENPEEPYLEGISYNCVAPGKRFMPMDNLSGGEKCVAALALLFAVHSFRPAPFFVLDEVDAALDNTNIGKVSSYIKEQTQDQFQMIVISLKEEFYSRADALIGIYPEYDDCMFSRVLTLDLSQYPDTEGQESSKRHGESR

Target ORF information:

RefSeq Version	NM_148674.4
Organism	Homo sapiens(human)
Definition	Homo sapiens structural maintenance of chromosomes 1B (SMC1B), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_148674.4

ORF Insert Sequence:

ATGGCCCACC TGGAGCTGCT GCTTGTGGAA AATTTCAAGT CGTGGCGGGG CCGCCAGGTC 
ATTGGCCCCT TCCGGAGGTT CACCTGCATC ATCGGCCCCA ACGGCTCTGG AAAATCTAAT 
GTAATGGATG CACTTAGTTT TGTAATGGGA GAGAAAATAG CTAATTTAAG AGTGAAAAAT 
ATTCAAGAAC TCATTCATGG AGCACATATT GGAAAACCTA TTTCTTCTTC TGCAAGTGTA 
AAAATTATAT ATGTGGAGGA AAGTGGCGAA GAGAAAACAT TTGCAAGGAT TATCCGAGGG 
GGATGCTCAG AATTTCGCTT TAATGATAAT CTTGTGAGTC GTTCTGTTTA CATTGCAGAG 
TTGGAAAAGA TAGGCATAAT AGTCAAAGCA CAAAATTGTT TGGTTTTTCA GGGAACTGTA 
GAGTCAATTT CAGTGAAGAA ACCCAAAGAA AGGACCCAGT TTTTTGAGGA AATCAGCACT 
TCAGGAGAGC TTATAGGAGA ATATGAAGAA AAGAAAAGAA AGTTACAAAA AGCCGAAGAG 
GATGCACAGT TTAACTTTAA TAAGAAAAAA AATATAGCGG CAGAGCGCAG ACAAGCAAAA 
TTAGAGAAGG AAGAGGCAGA ACGTTACCAG AGTCTCCTTG AAGAACTGAA AATGAACAAG 
ATACAACTGC AGCTTTTTCA ACTATACCAT AATGAGAAAA AGATTCATCT CCTGAACACC 
AAGTTAGAGC ATGTGAATAG GGATTTGAGT GTCAAAAGAG AGTCTTTGTC TCATCATGAA 
AACATAGTTA AAGCCAGGAA AAAGGAACAT GGAATGCTAA CTAGACAACT ACAACAAACA 
GAAAAAGAAT TAAAATCGGT TGAAACCCTT TTAAATCAGA AGAGGCCTCA GTACATTAAA 
GCCAAAGAAA ACACTTCTCA CCACCTTAAG AAATTAGATG TGGCTAAGAA ATCAATAAAG 
GACAGCGAAA AACAATGTTC TAAACAGGAA GATGATATAA AAGCCCTGGA GACAGAGCTG 
GCTGATTTAG ATGCTGCATG GAGAAGTTTT GAAAAGCAGA TTGAGGAAGA AATTTTACAT 
AAAAAGCGAG ACATTGAACT GGAAGCCAGT CAGCTGGATC GTTATAAAGA ACTTAAGGAA 
CAAGTAAGAA AGAAAGTAGC TACAATGACT CAACAACTGG AAAAACTGCA GTGGGAACAG 
AAGACAGATG AAGAAAGACT GGCATTTGAA AAGAGGAGGC ATGGAGAAGT TCAGGGAAAT 
CTAAAACAAA TAAAAGAACA AATAGAAGAT CATAAAAAAC GAATAGAGAA GTTAGAGGAG 
TATACAAAGA CATGCATGGA TTGCTTGAAA GAGAAAAAAC AGCAAGAGGA AACCCTAGTG 
GATGAAATTG AAAAAACAAA ATCAAGAATG TCTGAAGTTA ATGAAGAATT GAATCTTATT 
AGAAGTGAAT TGCAGAATGC TGGGATTGAT ACCCATGAGG GAAAACGTCA GCAAAAGAGA 
GCAGAGGTTC TGGAACACCT TAAAAGACTG TACCCAGATT CTGTGTTTGG AAGACTATTT 
GACCTGTGTC ATCCTATTCA TAAGAAATAC CAGCTGGCTG TTACTAAGGT TTTTGGCCGG 
TTCATCACTG CCATTGTTGT AGCCTCTGAA AAGGTAGCAA AAGATTGTAT TCGATTTCTG 
AAGGAGGAAA GAGCTGAACC TGAGACATTC CTCGCTCTAG ATTACCTTGA TATCAAGCCA 
ATCAATGAAA GACTAAGGGA GCTTAAAGGC TGTAAAATGG TGATTGATGT CATAAAGACT 
CAGTTTCCTC AGCTGAAGAA AGTGATTCAG TTTGTGTGTG GAAATGGTCT TGTTTGTGAG 
ACTATGGAAG AAGCAAGGCA TATTGCACTC AGTGGACCTG AAAGACAGAA AACAGTAGCT 
CTTGATGGAA CATTATTTTT AAAATCTGGA GTGATCTCTG GAGGGTCAAG TGACTTAAAA 
TACAAGGCTA GATGCTGGGA TGAGAAAGAG TTAAAGAATC TAAGAGACAG ACGAAGCCAG 
AAAATCCAAG AGCTAAAGGG TTTAATGAAG ACACTCCGCA AAGAAACAGA TTTGAAACAA 
ATACAGACCC TGATACAGGG AACTCAAACA CGACTCAAAT ATTCACAAAA TGAACTAGAG 
ATGATTAAGA AGAAGCACCT TGTTGCTTTT TACCAGGAAC AATCTCAGTT ACAAAGTGAA 
CTACTAAATA TTGAGTCTCA ATGTATTATG TTGAGTGAAG GAATCAAGGA ACGACAACGA 
AGAATTAAAG AATTTCAAGA AAAGATAGAT AAGGTAGAAG ACGATATCTT CCAACACTTC 
TGTGAAGAAA TTGGCGTGGA AAATATTCGT GAATTTGAGA ACAAACATGT TAAACGGCAA 
CAAGAAATTG ATCAAAAAAG ATTAGAATTT GAAAAACAAA AAACTCGGCT TAATGTTCAA 
CTTGAGTATA GTCGCAGTCA CCTTAAGAAG AAACTGAATA AGATCAACAC ATTAAAAGAA 
ACTATCCAGA AAGGTAGTGA AGATATTGAT CACCTAAAGA AGGCTGAAGA AAACTGTCTG 
CAGACAGTGA ATGAACTCAT GGCAAAGCAG CAGCAACTTA AGGACATACG TGTCACTCAG 
AACTCCAGTG CCGAGAAAGT TCAAACTCAA ATTGAAGAGG AACGGAAGAA GTTTCTGGCT 
GTTGATAGGG AAGTGGGGAA ATTGCAAAAA GAAGTTGTAA GTATTCAAAC TTCTCTGGAA 
CAGAAACGAT TAGAGAAGCA TAACTTGCTG CTTGATTGCA AAGTGCAAGA CATTGAGATA 
ATCCTTTTGT CGGGGTCACT GGATGACATC ATTGAAGTGG AGATGGGAAC TGAAGCAGAA 
AGTACCCAGG CAACAATTGA TATCTATGAA AAAGAAGAAG CCTTTGAAAT AGACTACAGC 
TCTCTAAAAG AGGATTTGAA GGCTCTACAG TCTGATCAAG AAATCGAGGC CCACCTTAGG 
CTCTTATTGC AGCAAGTAGC ATCCCAGGAA GATATCTTAC TGAAAACAGC AGCCCCAAAC 
CTACGAGCAC TGGAGAACTT AAAGACTGTC AGAGACAAGT TTCAAGAGTC CACAGATGCT 
TTTGAGGCCA GCAGAAAGGA AGCCAGACTG TGTAGGCAAG AGTTCGAGCA AGTGAAAAAA 
AGGAGATACG ATCTTTTCAC CCAGTGTTTT GAGCATGTCT CAATCTCAAT TGATCAAATC 
TACAAGAAGC TCTGCAGAAA CAACAGCGCC CAAGCATTTC TTAGCCCAGA GAACCCTGAA 
GAACCTTACT TGGAGGGAAT TAGCTATAAC TGTGTGGCCC CAGGCAAACG GTTTATGCCA 
ATGGACAATT TGTCAGGGGG AGAAAAGTGT GTGGCAGCCT TGGCTCTCCT GTTTGCTGTG 
CACAGTTTTC GTCCTGCCCC ATTCTTTGTT TTAGATGAAG TGGATGCAGC CCTAGACAAT 
ACTAACATAG GCAAAGTGTC AAGTTACATC AAAGAGCAAA CTCAAGACCA GTTTCAGATG 
ATAGTCATCT CCCTAAAAGA AGAGTTCTAT TCCAGAGCCG ACGCGCTGAT CGGCATCTAT 
CCTGAGTACG ATGACTGCAT GTTCAGCCGA GTTTTGACCC TAGATCTTTC TCAGTATCCA 
GACACTGAAG GCCAAGAAAG CAGCAAGAGA CACGGAGAGT CCCGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMC1B ( NM_148674.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_683515.4 Homo sapiens structural maintenance of chromosomes 1B (SMC1B), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu16114
Clone ID Related Accession (Same CDS sequence)	NM_148674.4 , NM_148674.5
Accession Version	NM_148674.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Homo sapiens(human)
Product	structural maintenance of chromosomes protein 1B isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC126208.1, AK125736.1, AJ504806.2 and BG194018.1. On Nov 23, 2018 this sequence version replaced NM_148674.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology MANE Ensembl match :: ENST00000357450.9/ ENSP00000350036.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCCACC TGGAGCTGCT GCTTGTGGAA AATTTCAAGT CGTGGCGGGG CCGCCAGGTC 
ATTGGCCCCT TCCGGAGGTT CACCTGCATC ATCGGCCCCA ACGGCTCTGG AAAATCTAAT 
GTAATGGATG CACTTAGTTT TGTAATGGGA GAGAAAATAG CTAATTTAAG AGTGAAAAAT 
ATTCAAGAAC TCATTCATGG AGCACATATT GGAAAACCTA TTTCTTCTTC TGCAAGTGTA 
AAAATTATAT ATGTGGAGGA AAGTGGCGAA GAGAAAACAT TTGCAAGGAT TATCCGAGGG 
GGATGCTCAG AATTTCGCTT TAATGATAAT CTTGTGAGTC GTTCTGTTTA CATTGCAGAG 
TTGGAAAAGA TAGGCATAAT AGTCAAAGCA CAAAATTGTT TGGTTTTTCA GGGAACTGTA 
GAGTCAATTT CAGTGAAGAA ACCCAAAGAA AGGACCCAGT TTTTTGAGGA AATCAGCACT 
TCAGGAGAGC TTATAGGAGA ATATGAAGAA AAGAAAAGAA AGTTACAAAA AGCCGAAGAG 
GATGCACAGT TTAACTTTAA TAAGAAAAAA AATATAGCGG CAGAGCGCAG ACAAGCAAAA 
TTAGAGAAGG AAGAGGCAGA ACGTTACCAG AGTCTCCTTG AAGAACTGAA AATGAACAAG 
ATACAACTGC AGCTTTTTCA ACTATACCAT AATGAGAAAA AGATTCATCT CCTGAACACC 
AAGTTAGAGC ATGTGAATAG GGATTTGAGT GTCAAAAGAG AGTCTTTGTC TCATCATGAA 
AACATAGTTA AAGCCAGGAA AAAGGAACAT GGAATGCTAA CTAGACAACT ACAACAAACA 
GAAAAAGAAT TAAAATCGGT TGAAACCCTT TTAAATCAGA AGAGGCCTCA GTACATTAAA 
GCCAAAGAAA ACACTTCTCA CCACCTTAAG AAATTAGATG TGGCTAAGAA ATCAATAAAG 
GACAGCGAAA AACAATGTTC TAAACAGGAA GATGATATAA AAGCCCTGGA GACAGAGCTG 
GCTGATTTAG ATGCTGCATG GAGAAGTTTT GAAAAGCAGA TTGAGGAAGA AATTTTACAT 
AAAAAGCGAG ACATTGAACT GGAAGCCAGT CAGCTGGATC GTTATAAAGA ACTTAAGGAA 
CAAGTAAGAA AGAAAGTAGC TACAATGACT CAACAACTGG AAAAACTGCA GTGGGAACAG 
AAGACAGATG AAGAAAGACT GGCATTTGAA AAGAGGAGGC ATGGAGAAGT TCAGGGAAAT 
CTAAAACAAA TAAAAGAACA AATAGAAGAT CATAAAAAAC GAATAGAGAA GTTAGAGGAG 
TATACAAAGA CATGCATGGA TTGCTTGAAA GAGAAAAAAC AGCAAGAGGA AACCCTAGTG 
GATGAAATTG AAAAAACAAA ATCAAGAATG TCTGAAGTTA ATGAAGAATT GAATCTTATT 
AGAAGTGAAT TGCAGAATGC TGGGATTGAT ACCCATGAGG GAAAACGTCA GCAAAAGAGA 
GCAGAGGTTC TGGAACACCT TAAAAGACTG TACCCAGATT CTGTGTTTGG AAGACTATTT 
GACCTGTGTC ATCCTATTCA TAAGAAATAC CAGCTGGCTG TTACTAAGGT TTTTGGCCGG 
TTCATCACTG CCATTGTTGT AGCCTCTGAA AAGGTAGCAA AAGATTGTAT TCGATTTCTG 
AAGGAGGAAA GAGCTGAACC TGAGACATTC CTCGCTCTAG ATTACCTTGA TATCAAGCCA 
ATCAATGAAA GACTAAGGGA GCTTAAAGGC TGTAAAATGG TGATTGATGT CATAAAGACT 
CAGTTTCCTC AGCTGAAGAA AGTGATTCAG TTTGTGTGTG GAAATGGTCT TGTTTGTGAG 
ACTATGGAAG AAGCAAGGCA TATTGCACTC AGTGGACCTG AAAGACAGAA AACAGTAGCT 
CTTGATGGAA CATTATTTTT AAAATCTGGA GTGATCTCTG GAGGGTCAAG TGACTTAAAA 
TACAAGGCTA GATGCTGGGA TGAGAAAGAG TTAAAGAATC TAAGAGACAG ACGAAGCCAG 
AAAATCCAAG AGCTAAAGGG TTTAATGAAG ACACTCCGCA AAGAAACAGA TTTGAAACAA 
ATACAGACCC TGATACAGGG AACTCAAACA CGACTCAAAT ATTCACAAAA TGAACTAGAG 
ATGATTAAGA AGAAGCACCT TGTTGCTTTT TACCAGGAAC AATCTCAGTT ACAAAGTGAA 
CTACTAAATA TTGAGTCTCA ATGTATTATG TTGAGTGAAG GAATCAAGGA ACGACAACGA 
AGAATTAAAG AATTTCAAGA AAAGATAGAT AAGGTAGAAG ACGATATCTT CCAACACTTC 
TGTGAAGAAA TTGGCGTGGA AAATATTCGT GAATTTGAGA ACAAACATGT TAAACGGCAA 
CAAGAAATTG ATCAAAAAAG ATTAGAATTT GAAAAACAAA AAACTCGGCT TAATGTTCAA 
CTTGAGTATA GTCGCAGTCA CCTTAAGAAG AAACTGAATA AGATCAACAC ATTAAAAGAA 
ACTATCCAGA AAGGTAGTGA AGATATTGAT CACCTAAAGA AGGCTGAAGA AAACTGTCTG 
CAGACAGTGA ATGAACTCAT GGCAAAGCAG CAGCAACTTA AGGACATACG TGTCACTCAG 
AACTCCAGTG CCGAGAAAGT TCAAACTCAA ATTGAAGAGG AACGGAAGAA GTTTCTGGCT 
GTTGATAGGG AAGTGGGGAA ATTGCAAAAA GAAGTTGTAA GTATTCAAAC TTCTCTGGAA 
CAGAAACGAT TAGAGAAGCA TAACTTGCTG CTTGATTGCA AAGTGCAAGA CATTGAGATA 
ATCCTTTTGT CGGGGTCACT GGATGACATC ATTGAAGTGG AGATGGGAAC TGAAGCAGAA 
AGTACCCAGG CAACAATTGA TATCTATGAA AAAGAAGAAG CCTTTGAAAT AGACTACAGC 
TCTCTAAAAG AGGATTTGAA GGCTCTACAG TCTGATCAAG AAATCGAGGC CCACCTTAGG 
CTCTTATTGC AGCAAGTAGC ATCCCAGGAA GATATCTTAC TGAAAACAGC AGCCCCAAAC 
CTACGAGCAC TGGAGAACTT AAAGACTGTC AGAGACAAGT TTCAAGAGTC CACAGATGCT 
TTTGAGGCCA GCAGAAAGGA AGCCAGACTG TGTAGGCAAG AGTTCGAGCA AGTGAAAAAA 
AGGAGATACG ATCTTTTCAC CCAGTGTTTT GAGCATGTCT CAATCTCAAT TGATCAAATC 
TACAAGAAGC TCTGCAGAAA CAACAGCGCC CAAGCATTTC TTAGCCCAGA GAACCCTGAA 
GAACCTTACT TGGAGGGAAT TAGCTATAAC TGTGTGGCCC CAGGCAAACG GTTTATGCCA 
ATGGACAATT TGTCAGGGGG AGAAAAGTGT GTGGCAGCCT TGGCTCTCCT GTTTGCTGTG 
CACAGTTTTC GTCCTGCCCC ATTCTTTGTT TTAGATGAAG TGGATGCAGC CCTAGACAAT 
ACTAACATAG GCAAAGTGTC AAGTTACATC AAAGAGCAAA CTCAAGACCA GTTTCAGATG 
ATAGTCATCT CCCTAAAAGA AGAGTTCTAT TCCAGAGCCG ACGCGCTGAT CGGCATCTAT 
CCTGAGTACG ATGACTGCAT GTTCAGCCGA GTTTTGACCC TAGATCTTTC TCAGTATCCA 
GACACTGAAG GCCAAGAAAG CAGCAAGAGA CACGGAGAGT CCCGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_683515.4
CDS	33..3740
Translation	MAHLELLLVENFKSWRGRQVIGPFRRFTCIIGPNGSGKSNVMDALSFVMGEKIANLRVKNIQELIHGAHIGKPISSSASVKIIYVEESGEEKTFARIIRGGCSEFRFNDNLVSRSVYIAELEKIGIIVKAQNCLVFQGTVESISVKKPKERTQFFEEISTSGELIGEYEEKKRKLQKAEEDAQFNFNKKKNIAAERRQAKLEKEEAERYQSLLEELKMNKIQLQLFQLYHNEKKIHLLNTKLEHVNRDLSVKRESLSHHENIVKARKKEHGMLTRQLQQTEKELKSVETLLNQKRPQYIKAKENTSHHLKKLDVAKKSIKDSEKQCSKQEDDIKALETELADLDAAWRSFEKQIEEEILHKKRDIELEASQLDRYKELKEQVRKKVATMTQQLEKLQWEQKTDEERLAFEKRRHGEVQGNLKQIKEQIEDHKKRIEKLEEYTKTCMDCLKEKKQQEETLVDEIEKTKSRMSEVNEELNLIRSELQNAGIDTHEGKRQQKRAEVLEHLKRLYPDSVFGRLFDLCHPIHKKYQLAVTKVFGRFITAIVVASEKVAKDCIRFLKEERAEPETFLALDYLDIKPINERLRELKGCKMVIDVIKTQFPQLKKVIQFVCGNGLVCETMEEARHIALSGPERQKTVALDGTLFLKSGVISGGSSDLKYKARCWDEKELKNLRDRRSQKIQELKGLMKTLRKETDLKQIQTLIQGTQTRLKYSQNELEMIKKKHLVAFYQEQSQLQSELLNIESQCIMLSEGIKERQRRIKEFQEKIDKVEDDIFQHFCEEIGVENIREFENKHVKRQQEIDQKRLEFEKQKTRLNVQLEYSRSHLKKKLNKINTLKETIQKGSEDIDHLKKAEENCLQTVNELMAKQQQLKDIRVTQNSSAEKVQTQIEEERKKFLAVDREVGKLQKEVVSIQTSLEQKRLEKHNLLLDCKVQDIEIILLSGSLDDIIEVEMGTEAESTQATIDIYEKEEAFEIDYSSLKEDLKALQSDQEIEAHLRLLLQQVASQEDILLKTAAPNLRALENLKTVRDKFQESTDAFEASRKEARLCRQEFEQVKKRRYDLFTQCFEHVSISIDQIYKKLCRNNSAQAFLSPENPEEPYLEGISYNCVAPGKRFMPMDNLSGGEKCVAALALLFAVHSFRPAPFFVLDEVDAALDNTNIGKVSSYIKEQTQDQFQMIVISLKEEFYSRADALIGIYPEYDDCMFSRVLTLDLSQYPDTEGQESSKRHGESR

Target ORF information:

RefSeq Version	NM_148674.5
Organism	Homo sapiens(human)
Definition	Homo sapiens structural maintenance of chromosomes 1B (SMC1B), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_148674.5

ORF Insert Sequence:

ATGGCCCACC TGGAGCTGCT GCTTGTGGAA AATTTCAAGT CGTGGCGGGG CCGCCAGGTC 
ATTGGCCCCT TCCGGAGGTT CACCTGCATC ATCGGCCCCA ACGGCTCTGG AAAATCTAAT 
GTAATGGATG CACTTAGTTT TGTAATGGGA GAGAAAATAG CTAATTTAAG AGTGAAAAAT 
ATTCAAGAAC TCATTCATGG AGCACATATT GGAAAACCTA TTTCTTCTTC TGCAAGTGTA 
AAAATTATAT ATGTGGAGGA AAGTGGCGAA GAGAAAACAT TTGCAAGGAT TATCCGAGGG 
GGATGCTCAG AATTTCGCTT TAATGATAAT CTTGTGAGTC GTTCTGTTTA CATTGCAGAG 
TTGGAAAAGA TAGGCATAAT AGTCAAAGCA CAAAATTGTT TGGTTTTTCA GGGAACTGTA 
GAGTCAATTT CAGTGAAGAA ACCCAAAGAA AGGACCCAGT TTTTTGAGGA AATCAGCACT 
TCAGGAGAGC TTATAGGAGA ATATGAAGAA AAGAAAAGAA AGTTACAAAA AGCCGAAGAG 
GATGCACAGT TTAACTTTAA TAAGAAAAAA AATATAGCGG CAGAGCGCAG ACAAGCAAAA 
TTAGAGAAGG AAGAGGCAGA ACGTTACCAG AGTCTCCTTG AAGAACTGAA AATGAACAAG 
ATACAACTGC AGCTTTTTCA ACTATACCAT AATGAGAAAA AGATTCATCT CCTGAACACC 
AAGTTAGAGC ATGTGAATAG GGATTTGAGT GTCAAAAGAG AGTCTTTGTC TCATCATGAA 
AACATAGTTA AAGCCAGGAA AAAGGAACAT GGAATGCTAA CTAGACAACT ACAACAAACA 
GAAAAAGAAT TAAAATCGGT TGAAACCCTT TTAAATCAGA AGAGGCCTCA GTACATTAAA 
GCCAAAGAAA ACACTTCTCA CCACCTTAAG AAATTAGATG TGGCTAAGAA ATCAATAAAG 
GACAGCGAAA AACAATGTTC TAAACAGGAA GATGATATAA AAGCCCTGGA GACAGAGCTG 
GCTGATTTAG ATGCTGCATG GAGAAGTTTT GAAAAGCAGA TTGAGGAAGA AATTTTACAT 
AAAAAGCGAG ACATTGAACT GGAAGCCAGT CAGCTGGATC GTTATAAAGA ACTTAAGGAA 
CAAGTAAGAA AGAAAGTAGC TACAATGACT CAACAACTGG AAAAACTGCA GTGGGAACAG 
AAGACAGATG AAGAAAGACT GGCATTTGAA AAGAGGAGGC ATGGAGAAGT TCAGGGAAAT 
CTAAAACAAA TAAAAGAACA AATAGAAGAT CATAAAAAAC GAATAGAGAA GTTAGAGGAG 
TATACAAAGA CATGCATGGA TTGCTTGAAA GAGAAAAAAC AGCAAGAGGA AACCCTAGTG 
GATGAAATTG AAAAAACAAA ATCAAGAATG TCTGAAGTTA ATGAAGAATT GAATCTTATT 
AGAAGTGAAT TGCAGAATGC TGGGATTGAT ACCCATGAGG GAAAACGTCA GCAAAAGAGA 
GCAGAGGTTC TGGAACACCT TAAAAGACTG TACCCAGATT CTGTGTTTGG AAGACTATTT 
GACCTGTGTC ATCCTATTCA TAAGAAATAC CAGCTGGCTG TTACTAAGGT TTTTGGCCGG 
TTCATCACTG CCATTGTTGT AGCCTCTGAA AAGGTAGCAA AAGATTGTAT TCGATTTCTG 
AAGGAGGAAA GAGCTGAACC TGAGACATTC CTCGCTCTAG ATTACCTTGA TATCAAGCCA 
ATCAATGAAA GACTAAGGGA GCTTAAAGGC TGTAAAATGG TGATTGATGT CATAAAGACT 
CAGTTTCCTC AGCTGAAGAA AGTGATTCAG TTTGTGTGTG GAAATGGTCT TGTTTGTGAG 
ACTATGGAAG AAGCAAGGCA TATTGCACTC AGTGGACCTG AAAGACAGAA AACAGTAGCT 
CTTGATGGAA CATTATTTTT AAAATCTGGA GTGATCTCTG GAGGGTCAAG TGACTTAAAA 
TACAAGGCTA GATGCTGGGA TGAGAAAGAG TTAAAGAATC TAAGAGACAG ACGAAGCCAG 
AAAATCCAAG AGCTAAAGGG TTTAATGAAG ACACTCCGCA AAGAAACAGA TTTGAAACAA 
ATACAGACCC TGATACAGGG AACTCAAACA CGACTCAAAT ATTCACAAAA TGAACTAGAG 
ATGATTAAGA AGAAGCACCT TGTTGCTTTT TACCAGGAAC AATCTCAGTT ACAAAGTGAA 
CTACTAAATA TTGAGTCTCA ATGTATTATG TTGAGTGAAG GAATCAAGGA ACGACAACGA 
AGAATTAAAG AATTTCAAGA AAAGATAGAT AAGGTAGAAG ACGATATCTT CCAACACTTC 
TGTGAAGAAA TTGGCGTGGA AAATATTCGT GAATTTGAGA ACAAACATGT TAAACGGCAA 
CAAGAAATTG ATCAAAAAAG ATTAGAATTT GAAAAACAAA AAACTCGGCT TAATGTTCAA 
CTTGAGTATA GTCGCAGTCA CCTTAAGAAG AAACTGAATA AGATCAACAC ATTAAAAGAA 
ACTATCCAGA AAGGTAGTGA AGATATTGAT CACCTAAAGA AGGCTGAAGA AAACTGTCTG 
CAGACAGTGA ATGAACTCAT GGCAAAGCAG CAGCAACTTA AGGACATACG TGTCACTCAG 
AACTCCAGTG CCGAGAAAGT TCAAACTCAA ATTGAAGAGG AACGGAAGAA GTTTCTGGCT 
GTTGATAGGG AAGTGGGGAA ATTGCAAAAA GAAGTTGTAA GTATTCAAAC TTCTCTGGAA 
CAGAAACGAT TAGAGAAGCA TAACTTGCTG CTTGATTGCA AAGTGCAAGA CATTGAGATA 
ATCCTTTTGT CGGGGTCACT GGATGACATC ATTGAAGTGG AGATGGGAAC TGAAGCAGAA 
AGTACCCAGG CAACAATTGA TATCTATGAA AAAGAAGAAG CCTTTGAAAT AGACTACAGC 
TCTCTAAAAG AGGATTTGAA GGCTCTACAG TCTGATCAAG AAATCGAGGC CCACCTTAGG 
CTCTTATTGC AGCAAGTAGC ATCCCAGGAA GATATCTTAC TGAAAACAGC AGCCCCAAAC 
CTACGAGCAC TGGAGAACTT AAAGACTGTC AGAGACAAGT TTCAAGAGTC CACAGATGCT 
TTTGAGGCCA GCAGAAAGGA AGCCAGACTG TGTAGGCAAG AGTTCGAGCA AGTGAAAAAA 
AGGAGATACG ATCTTTTCAC CCAGTGTTTT GAGCATGTCT CAATCTCAAT TGATCAAATC 
TACAAGAAGC TCTGCAGAAA CAACAGCGCC CAAGCATTTC TTAGCCCAGA GAACCCTGAA 
GAACCTTACT TGGAGGGAAT TAGCTATAAC TGTGTGGCCC CAGGCAAACG GTTTATGCCA 
ATGGACAATT TGTCAGGGGG AGAAAAGTGT GTGGCAGCCT TGGCTCTCCT GTTTGCTGTG 
CACAGTTTTC GTCCTGCCCC ATTCTTTGTT TTAGATGAAG TGGATGCAGC CCTAGACAAT 
ACTAACATAG GCAAAGTGTC AAGTTACATC AAAGAGCAAA CTCAAGACCA GTTTCAGATG 
ATAGTCATCT CCCTAAAAGA AGAGTTCTAT TCCAGAGCCG ACGCGCTGAT CGGCATCTAT 
CCTGAGTACG ATGACTGCAT GTTCAGCCGA GTTTTGACCC TAGATCTTTC TCAGTATCCA 
GACACTGAAG GCCAAGAAAG CAGCAAGAGA CACGGAGAGT CCCGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMC1B ( NM_001291501.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001278430.1 Homo sapiens structural maintenance of chromosomes 1B (SMC1B), transcript variant 2, mRNA.

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CloneID	OHu16115
Clone ID Related Accession (Same CDS sequence)	NM_001291501.1 , NM_001291501.2
Accession Version	NM_001291501.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-23
Organism	Homo sapiens(human)
Product	structural maintenance of chromosomes protein 1B isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AJ504806.2, BC126208.1 and BG194018.1. On Apr 18, 2014 this sequence version replaced XM_005261566.2. Transcript Variant: This variant (2) lacks two alternate in-frame exons in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC126208.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCACC TGGAGCTGCT GCTTGTGGAA AATTTCAAGT CGTGGCGGGG CCGCCAGGTC 
ATTGGCCCCT TCCGGAGGTT CACCTGCATC ATCGGCCCCA ACGGCTCTGG AAAATCTAAT 
GTAATGGATG CACTTAGTTT TGTAATGGGA GAGAAAATAG CTAATTTAAG AGTGAAAAAT 
ATTCAAGAAC TCATTCATGG AGCACATATT GGAAAACCTA TTTCTTCTTC TGCAAGTGTA 
AAAATTATAT ATGTGGAGGA AAGTGGCGAA GAGAAAACAT TTGCAAGGAT TATCCGAGGG 
GGATGCTCAG AATTTCGCTT TAATGATAAT CTTGTGAGTC GTTCTGTTTA CATTGCAGAG 
TTGGAAAAGA TAGGCATAAT AGTCAAAGCA CAAAATTGTT TGGTTTTTCA GGGAACTGTA 
GAGTCAATTT CAGTGAAGAA ACCCAAAGAA AGGACCCAGT TTTTTGAGGA AATCAGCACT 
TCAGGAGAGC TTATAGGAGA ATATGAAGAA AAGAAAAGAA AGTTACAAAA AGCCGAAGAG 
GATGCACAGT TTAACTTTAA TAAGAAAAAA AATATAGCGG CAGAGCGCAG ACAAGCAAAA 
TTAGAGAAGG AAGAGGCAGA ACGTTACCAG AGTCTCCTTG AAGAACTGAA AATGAACAAG 
ATACAACTGC AGCTTTTTCA ACTATACCAT AATGAGAAAA AGATTCATCT CCTGAACACC 
AAGTTAGAGC ATGTGAATAG GGATTTGAGT GTCAAAAGAG AGTCTTTGTC TCATCATGAA 
AACATAGTTA AAGCCAGGAA AAAGGAACAT GGAATGCTAA CTAGACAACT ACAACAAACA 
GAAAAAGAAT TAAAATCGGT TGAAACCCTT TTAAATCAGA AGAGGCCTCA GTACATTAAA 
GCCAAAGAAA ACACTTCTCA CCACCTTAAG AAATTAGATG TGGCTAAGAA ATCAATAAAG 
GACAGCGAAA AACAATGTTC TAAACAGGAA GATGATATAA AAGCCCTGGA GACAGAGCTG 
GCTGATTTAG ATGCTGCATG GAGAAGTTTT GAAAAGCAGA TTGAGGAAGA AATTTTACAT 
AAAAAGCGAG ACATTGAACT GGAAGCCAGT CAGCTGGATC GTTATAAAGA ACTTAAGGAA 
CAAGTAAGAA AGAAAGTAGC TACAATGACT CAACAACTGG AAAAACTGCA GTGGGAACAG 
AAGACAGATG AAGAAAGACT GGCATTTGAA AAGAGGAGGC ATGGAGAAGT TCAGGGAAAT 
CTAAAACAAA TAAAAGAACA AATAGAAGAT CATAAAAAAC GAATAGAGAA GTTAGAGGAG 
TATACAAAGA CATGCATGGA TTGCTTGAAA GAGAAAAAAC AGCAAGAGGA AACCCTAGTG 
GATGAAATTG AAAAAACAAA ATCAAGAATG TCTGAAGTTA ATGAAGAATT GAATCTTATT 
AGAAGTGAAT TGCAGAATGC TGGGATTGAT ACCCATGAGG GAAAACGTCA GCAAAAGAGA 
GCAGAGGTTC TGGAACACCT TAAAAGACTG TACCCAGATT CTGTGTTTGG AAGACTATTT 
GACCTGTGTC ATCCTATTCA TAAGAAATAC CAGCTGGCTG TTACTAAGGT TTTTGGCCGG 
TTCATCACTG CCATTGTTGT AGCCTCTGAA AAGGTAGCAA AAGATTGTAT TCGATTTCTG 
AAGGAGGAAA GAGCTGAACC TGAGACATTC CTCGCTCTAG ATTACCTTGA TATCAAGCCA 
ATCAATGAAA GACTAAGGGA GCTTAAAGGC TGTAAAATGG TGATTGATGT CATAAAGACT 
CAGTTTCCTC AGCTGAAGAA AGTGATTCAG TTTGTGTGTG GAAATGGTCT TGTTTGTGAG 
ACTATGGAAG AAGCAAGGCA TATTGCACTC AGTGGACCTG AAAGACAGAA AACAGTAGCT 
CTTGATGGAA CATTATTTTT AAAATCTGGA GTGATCTCTG GAGGGTCAAG TGACTTAAAA 
TACAAGGCTA GATGCTGGGA TGAGAAAGAG TTAAAGAATC TAAGAGACAG ACGAAGCCAG 
AAAATCCAAG AGCTAAAGGG TTTAATGAAG ACACTCCGCA AAGAAACAGA TTTGAAACAA 
ATACAGACCC TGATACAGGG AACTCAAACA CGACTCAAAT ATTCACAAAA TGAACTAGAG 
ATGATTAAGA AGAAGCACCT TGTTGCTTTT TACCAGGAAC AATCTCAGTT ACAAAGTGAA 
CTACTAAATA TTGAGTCTCA ATGTATTATG TTGAGTGAAG GAATCAAGGA ACGACAACGA 
AGAATTAAAG AATTTCAAGA AAAGATAGAT AAGGTAGAAG ACGATATCTT CCAACACTTC 
TGTGAAGAAA TTGGCGTGGA AAATATTCGT GAATTTGAGA ACAAACATGT TAAACGGCAA 
CAAGAAATTG ATCAAAAAAG ATTAGAATTT GAAAAACAAA AAACTCGGCT TAATGTTCAA 
CTTGAGTATA GTCGCAGTCA CCTTAAGAAG AAACTGAATA AGATCAACAC ATTAAAAGAA 
ACTATCCAGA AAGGTAGTGA AGATATTGAT CACCTAAAGA AGGCTGAAGA AAACTGTCTG 
CAGACAGTGA ATGAACTCAT GGCAAAGCAG CAGCAACTTA AGGACATACG TGTCACTCAG 
AACTCCAGTG CCGAGAAAGT TCAAACTCAA ATTGAAGAGG AACGGAAGAA GTTTCTGGCT 
GTTGATAGGG AAGTGGGGAA ATTGCAAAAA GAAGTTGTAA GTATTCAAAC TTCTCTGGAA 
CAGAAACGAT TAGAGAAGCA TAACTTGCTG CTTGATTGCA AAGTGCAAGA CATTGAGATA 
ATCCTTTTGT CGGGGTCACT GGATGACATC ATTGAAGTGG AGATGGGAAC TGAAGCAGAA 
AGTACCCAGG CAACAATTGA TATCTATGAA AAAGAAGAAG CCTTTGAAAT AGACTACAGC 
TCTCTAAAAG AGGATTTGAA GGCTCTACAG TCTGATCAAG AAATCGAGGC CCACCTTAGG 
CTCTTATTGC AGCAAGTAGC ATCCCAGGAA GATATCTTAC TGAAAACAGC AGCCCCAAAC 
CTACGAGCAC TGGAGAACTT AAAGACTGTC AGAGACAAGT TTCAAGAGTC CACAGATGCT 
TTTGAGGCCA GCAGAAAGGA AGCCAGACTG TGTAGGCAAG AGTTCGAGCA AGTGAAAAAA 
AGGAGATACG ATCTTTTCAC CCAGTGTTTT GAGCATGTCT CAATCTCAAT TGATCAAATC 
TACAAGAAGC TCTGCAGAAA CAACAGCGCC CAAGTGTCAA GTTACATCAA AGAGCAAACT 
CAAGACCAGT TTCAGATGAT AGTCATCTCC CTAAAAGAAG AGTTCTATTC CAGAGCCGAC 
GCGCTGATCG GCATCTATCC TGAGTACGAT GACTGCATGT TCAGCCGAGT TTTGACCCTA 
GATCTTTCTC AGTATCCAGA CACTGAAGGC CAAGAAAGCA GCAAGAGACA CGGAGAGTCC 
CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278430.1
CDS	53..3538
Translation	MAHLELLLVENFKSWRGRQVIGPFRRFTCIIGPNGSGKSNVMDALSFVMGEKIANLRVKNIQELIHGAHIGKPISSSASVKIIYVEESGEEKTFARIIRGGCSEFRFNDNLVSRSVYIAELEKIGIIVKAQNCLVFQGTVESISVKKPKERTQFFEEISTSGELIGEYEEKKRKLQKAEEDAQFNFNKKKNIAAERRQAKLEKEEAERYQSLLEELKMNKIQLQLFQLYHNEKKIHLLNTKLEHVNRDLSVKRESLSHHENIVKARKKEHGMLTRQLQQTEKELKSVETLLNQKRPQYIKAKENTSHHLKKLDVAKKSIKDSEKQCSKQEDDIKALETELADLDAAWRSFEKQIEEEILHKKRDIELEASQLDRYKELKEQVRKKVATMTQQLEKLQWEQKTDEERLAFEKRRHGEVQGNLKQIKEQIEDHKKRIEKLEEYTKTCMDCLKEKKQQEETLVDEIEKTKSRMSEVNEELNLIRSELQNAGIDTHEGKRQQKRAEVLEHLKRLYPDSVFGRLFDLCHPIHKKYQLAVTKVFGRFITAIVVASEKVAKDCIRFLKEERAEPETFLALDYLDIKPINERLRELKGCKMVIDVIKTQFPQLKKVIQFVCGNGLVCETMEEARHIALSGPERQKTVALDGTLFLKSGVISGGSSDLKYKARCWDEKELKNLRDRRSQKIQELKGLMKTLRKETDLKQIQTLIQGTQTRLKYSQNELEMIKKKHLVAFYQEQSQLQSELLNIESQCIMLSEGIKERQRRIKEFQEKIDKVEDDIFQHFCEEIGVENIREFENKHVKRQQEIDQKRLEFEKQKTRLNVQLEYSRSHLKKKLNKINTLKETIQKGSEDIDHLKKAEENCLQTVNELMAKQQQLKDIRVTQNSSAEKVQTQIEEERKKFLAVDREVGKLQKEVVSIQTSLEQKRLEKHNLLLDCKVQDIEIILLSGSLDDIIEVEMGTEAESTQATIDIYEKEEAFEIDYSSLKEDLKALQSDQEIEAHLRLLLQQVASQEDILLKTAAPNLRALENLKTVRDKFQESTDAFEASRKEARLCRQEFEQVKKRRYDLFTQCFEHVSISIDQIYKKLCRNNSAQVSSYIKEQTQDQFQMIVISLKEEFYSRADALIGIYPEYDDCMFSRVLTLDLSQYPDTEGQESSKRHGESR

Target ORF information:

RefSeq Version	NM_001291501.1
Organism	Homo sapiens(human)
Definition	Homo sapiens structural maintenance of chromosomes 1B (SMC1B), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001291501.1

ORF Insert Sequence:

ATGGCCCACC TGGAGCTGCT GCTTGTGGAA AATTTCAAGT CGTGGCGGGG CCGCCAGGTC 
ATTGGCCCCT TCCGGAGGTT CACCTGCATC ATCGGCCCCA ACGGCTCTGG AAAATCTAAT 
GTAATGGATG CACTTAGTTT TGTAATGGGA GAGAAAATAG CTAATTTAAG AGTGAAAAAT 
ATTCAAGAAC TCATTCATGG AGCACATATT GGAAAACCTA TTTCTTCTTC TGCAAGTGTA 
AAAATTATAT ATGTGGAGGA AAGTGGCGAA GAGAAAACAT TTGCAAGGAT TATCCGAGGG 
GGATGCTCAG AATTTCGCTT TAATGATAAT CTTGTGAGTC GTTCTGTTTA CATTGCAGAG 
TTGGAAAAGA TAGGCATAAT AGTCAAAGCA CAAAATTGTT TGGTTTTTCA GGGAACTGTA 
GAGTCAATTT CAGTGAAGAA ACCCAAAGAA AGGACCCAGT TTTTTGAGGA AATCAGCACT 
TCAGGAGAGC TTATAGGAGA ATATGAAGAA AAGAAAAGAA AGTTACAAAA AGCCGAAGAG 
GATGCACAGT TTAACTTTAA TAAGAAAAAA AATATAGCGG CAGAGCGCAG ACAAGCAAAA 
TTAGAGAAGG AAGAGGCAGA ACGTTACCAG AGTCTCCTTG AAGAACTGAA AATGAACAAG 
ATACAACTGC AGCTTTTTCA ACTATACCAT AATGAGAAAA AGATTCATCT CCTGAACACC 
AAGTTAGAGC ATGTGAATAG GGATTTGAGT GTCAAAAGAG AGTCTTTGTC TCATCATGAA 
AACATAGTTA AAGCCAGGAA AAAGGAACAT GGAATGCTAA CTAGACAACT ACAACAAACA 
GAAAAAGAAT TAAAATCGGT TGAAACCCTT TTAAATCAGA AGAGGCCTCA GTACATTAAA 
GCCAAAGAAA ACACTTCTCA CCACCTTAAG AAATTAGATG TGGCTAAGAA ATCAATAAAG 
GACAGCGAAA AACAATGTTC TAAACAGGAA GATGATATAA AAGCCCTGGA GACAGAGCTG 
GCTGATTTAG ATGCTGCATG GAGAAGTTTT GAAAAGCAGA TTGAGGAAGA AATTTTACAT 
AAAAAGCGAG ACATTGAACT GGAAGCCAGT CAGCTGGATC GTTATAAAGA ACTTAAGGAA 
CAAGTAAGAA AGAAAGTAGC TACAATGACT CAACAACTGG AAAAACTGCA GTGGGAACAG 
AAGACAGATG AAGAAAGACT GGCATTTGAA AAGAGGAGGC ATGGAGAAGT TCAGGGAAAT 
CTAAAACAAA TAAAAGAACA AATAGAAGAT CATAAAAAAC GAATAGAGAA GTTAGAGGAG 
TATACAAAGA CATGCATGGA TTGCTTGAAA GAGAAAAAAC AGCAAGAGGA AACCCTAGTG 
GATGAAATTG AAAAAACAAA ATCAAGAATG TCTGAAGTTA ATGAAGAATT GAATCTTATT 
AGAAGTGAAT TGCAGAATGC TGGGATTGAT ACCCATGAGG GAAAACGTCA GCAAAAGAGA 
GCAGAGGTTC TGGAACACCT TAAAAGACTG TACCCAGATT CTGTGTTTGG AAGACTATTT 
GACCTGTGTC ATCCTATTCA TAAGAAATAC CAGCTGGCTG TTACTAAGGT TTTTGGCCGG 
TTCATCACTG CCATTGTTGT AGCCTCTGAA AAGGTAGCAA AAGATTGTAT TCGATTTCTG 
AAGGAGGAAA GAGCTGAACC TGAGACATTC CTCGCTCTAG ATTACCTTGA TATCAAGCCA 
ATCAATGAAA GACTAAGGGA GCTTAAAGGC TGTAAAATGG TGATTGATGT CATAAAGACT 
CAGTTTCCTC AGCTGAAGAA AGTGATTCAG TTTGTGTGTG GAAATGGTCT TGTTTGTGAG 
ACTATGGAAG AAGCAAGGCA TATTGCACTC AGTGGACCTG AAAGACAGAA AACAGTAGCT 
CTTGATGGAA CATTATTTTT AAAATCTGGA GTGATCTCTG GAGGGTCAAG TGACTTAAAA 
TACAAGGCTA GATGCTGGGA TGAGAAAGAG TTAAAGAATC TAAGAGACAG ACGAAGCCAG 
AAAATCCAAG AGCTAAAGGG TTTAATGAAG ACACTCCGCA AAGAAACAGA TTTGAAACAA 
ATACAGACCC TGATACAGGG AACTCAAACA CGACTCAAAT ATTCACAAAA TGAACTAGAG 
ATGATTAAGA AGAAGCACCT TGTTGCTTTT TACCAGGAAC AATCTCAGTT ACAAAGTGAA 
CTACTAAATA TTGAGTCTCA ATGTATTATG TTGAGTGAAG GAATCAAGGA ACGACAACGA 
AGAATTAAAG AATTTCAAGA AAAGATAGAT AAGGTAGAAG ACGATATCTT CCAACACTTC 
TGTGAAGAAA TTGGCGTGGA AAATATTCGT GAATTTGAGA ACAAACATGT TAAACGGCAA 
CAAGAAATTG ATCAAAAAAG ATTAGAATTT GAAAAACAAA AAACTCGGCT TAATGTTCAA 
CTTGAGTATA GTCGCAGTCA CCTTAAGAAG AAACTGAATA AGATCAACAC ATTAAAAGAA 
ACTATCCAGA AAGGTAGTGA AGATATTGAT CACCTAAAGA AGGCTGAAGA AAACTGTCTG 
CAGACAGTGA ATGAACTCAT GGCAAAGCAG CAGCAACTTA AGGACATACG TGTCACTCAG 
AACTCCAGTG CCGAGAAAGT TCAAACTCAA ATTGAAGAGG AACGGAAGAA GTTTCTGGCT 
GTTGATAGGG AAGTGGGGAA ATTGCAAAAA GAAGTTGTAA GTATTCAAAC TTCTCTGGAA 
CAGAAACGAT TAGAGAAGCA TAACTTGCTG CTTGATTGCA AAGTGCAAGA CATTGAGATA 
ATCCTTTTGT CGGGGTCACT GGATGACATC ATTGAAGTGG AGATGGGAAC TGAAGCAGAA 
AGTACCCAGG CAACAATTGA TATCTATGAA AAAGAAGAAG CCTTTGAAAT AGACTACAGC 
TCTCTAAAAG AGGATTTGAA GGCTCTACAG TCTGATCAAG AAATCGAGGC CCACCTTAGG 
CTCTTATTGC AGCAAGTAGC ATCCCAGGAA GATATCTTAC TGAAAACAGC AGCCCCAAAC 
CTACGAGCAC TGGAGAACTT AAAGACTGTC AGAGACAAGT TTCAAGAGTC CACAGATGCT 
TTTGAGGCCA GCAGAAAGGA AGCCAGACTG TGTAGGCAAG AGTTCGAGCA AGTGAAAAAA 
AGGAGATACG ATCTTTTCAC CCAGTGTTTT GAGCATGTCT CAATCTCAAT TGATCAAATC 
TACAAGAAGC TCTGCAGAAA CAACAGCGCC CAAGTGTCAA GTTACATCAA AGAGCAAACT 
CAAGACCAGT TTCAGATGAT AGTCATCTCC CTAAAAGAAG AGTTCTATTC CAGAGCCGAC 
GCGCTGATCG GCATCTATCC TGAGTACGAT GACTGCATGT TCAGCCGAGT TTTGACCCTA 
GATCTTTCTC AGTATCCAGA CACTGAAGGC CAAGAAAGCA GCAAGAGACA CGGAGAGTCC 
CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMC1B ( NM_001291501.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001278430.1 Homo sapiens structural maintenance of chromosomes 1B (SMC1B), transcript variant 2, mRNA.

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CloneID	OHu16115
Clone ID Related Accession (Same CDS sequence)	NM_001291501.1 , NM_001291501.2
Accession Version	NM_001291501.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	structural maintenance of chromosomes protein 1B isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC126208.1, AJ504806.2 and BG194018.1. On May 31, 2019 this sequence version replaced NM_001291501.1. Transcript Variant: This variant (2) lacks two alternate in-frame exons in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC126208.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCACC TGGAGCTGCT GCTTGTGGAA AATTTCAAGT CGTGGCGGGG CCGCCAGGTC 
ATTGGCCCCT TCCGGAGGTT CACCTGCATC ATCGGCCCCA ACGGCTCTGG AAAATCTAAT 
GTAATGGATG CACTTAGTTT TGTAATGGGA GAGAAAATAG CTAATTTAAG AGTGAAAAAT 
ATTCAAGAAC TCATTCATGG AGCACATATT GGAAAACCTA TTTCTTCTTC TGCAAGTGTA 
AAAATTATAT ATGTGGAGGA AAGTGGCGAA GAGAAAACAT TTGCAAGGAT TATCCGAGGG 
GGATGCTCAG AATTTCGCTT TAATGATAAT CTTGTGAGTC GTTCTGTTTA CATTGCAGAG 
TTGGAAAAGA TAGGCATAAT AGTCAAAGCA CAAAATTGTT TGGTTTTTCA GGGAACTGTA 
GAGTCAATTT CAGTGAAGAA ACCCAAAGAA AGGACCCAGT TTTTTGAGGA AATCAGCACT 
TCAGGAGAGC TTATAGGAGA ATATGAAGAA AAGAAAAGAA AGTTACAAAA AGCCGAAGAG 
GATGCACAGT TTAACTTTAA TAAGAAAAAA AATATAGCGG CAGAGCGCAG ACAAGCAAAA 
TTAGAGAAGG AAGAGGCAGA ACGTTACCAG AGTCTCCTTG AAGAACTGAA AATGAACAAG 
ATACAACTGC AGCTTTTTCA ACTATACCAT AATGAGAAAA AGATTCATCT CCTGAACACC 
AAGTTAGAGC ATGTGAATAG GGATTTGAGT GTCAAAAGAG AGTCTTTGTC TCATCATGAA 
AACATAGTTA AAGCCAGGAA AAAGGAACAT GGAATGCTAA CTAGACAACT ACAACAAACA 
GAAAAAGAAT TAAAATCGGT TGAAACCCTT TTAAATCAGA AGAGGCCTCA GTACATTAAA 
GCCAAAGAAA ACACTTCTCA CCACCTTAAG AAATTAGATG TGGCTAAGAA ATCAATAAAG 
GACAGCGAAA AACAATGTTC TAAACAGGAA GATGATATAA AAGCCCTGGA GACAGAGCTG 
GCTGATTTAG ATGCTGCATG GAGAAGTTTT GAAAAGCAGA TTGAGGAAGA AATTTTACAT 
AAAAAGCGAG ACATTGAACT GGAAGCCAGT CAGCTGGATC GTTATAAAGA ACTTAAGGAA 
CAAGTAAGAA AGAAAGTAGC TACAATGACT CAACAACTGG AAAAACTGCA GTGGGAACAG 
AAGACAGATG AAGAAAGACT GGCATTTGAA AAGAGGAGGC ATGGAGAAGT TCAGGGAAAT 
CTAAAACAAA TAAAAGAACA AATAGAAGAT CATAAAAAAC GAATAGAGAA GTTAGAGGAG 
TATACAAAGA CATGCATGGA TTGCTTGAAA GAGAAAAAAC AGCAAGAGGA AACCCTAGTG 
GATGAAATTG AAAAAACAAA ATCAAGAATG TCTGAAGTTA ATGAAGAATT GAATCTTATT 
AGAAGTGAAT TGCAGAATGC TGGGATTGAT ACCCATGAGG GAAAACGTCA GCAAAAGAGA 
GCAGAGGTTC TGGAACACCT TAAAAGACTG TACCCAGATT CTGTGTTTGG AAGACTATTT 
GACCTGTGTC ATCCTATTCA TAAGAAATAC CAGCTGGCTG TTACTAAGGT TTTTGGCCGG 
TTCATCACTG CCATTGTTGT AGCCTCTGAA AAGGTAGCAA AAGATTGTAT TCGATTTCTG 
AAGGAGGAAA GAGCTGAACC TGAGACATTC CTCGCTCTAG ATTACCTTGA TATCAAGCCA 
ATCAATGAAA GACTAAGGGA GCTTAAAGGC TGTAAAATGG TGATTGATGT CATAAAGACT 
CAGTTTCCTC AGCTGAAGAA AGTGATTCAG TTTGTGTGTG GAAATGGTCT TGTTTGTGAG 
ACTATGGAAG AAGCAAGGCA TATTGCACTC AGTGGACCTG AAAGACAGAA AACAGTAGCT 
CTTGATGGAA CATTATTTTT AAAATCTGGA GTGATCTCTG GAGGGTCAAG TGACTTAAAA 
TACAAGGCTA GATGCTGGGA TGAGAAAGAG TTAAAGAATC TAAGAGACAG ACGAAGCCAG 
AAAATCCAAG AGCTAAAGGG TTTAATGAAG ACACTCCGCA AAGAAACAGA TTTGAAACAA 
ATACAGACCC TGATACAGGG AACTCAAACA CGACTCAAAT ATTCACAAAA TGAACTAGAG 
ATGATTAAGA AGAAGCACCT TGTTGCTTTT TACCAGGAAC AATCTCAGTT ACAAAGTGAA 
CTACTAAATA TTGAGTCTCA ATGTATTATG TTGAGTGAAG GAATCAAGGA ACGACAACGA 
AGAATTAAAG AATTTCAAGA AAAGATAGAT AAGGTAGAAG ACGATATCTT CCAACACTTC 
TGTGAAGAAA TTGGCGTGGA AAATATTCGT GAATTTGAGA ACAAACATGT TAAACGGCAA 
CAAGAAATTG ATCAAAAAAG ATTAGAATTT GAAAAACAAA AAACTCGGCT TAATGTTCAA 
CTTGAGTATA GTCGCAGTCA CCTTAAGAAG AAACTGAATA AGATCAACAC ATTAAAAGAA 
ACTATCCAGA AAGGTAGTGA AGATATTGAT CACCTAAAGA AGGCTGAAGA AAACTGTCTG 
CAGACAGTGA ATGAACTCAT GGCAAAGCAG CAGCAACTTA AGGACATACG TGTCACTCAG 
AACTCCAGTG CCGAGAAAGT TCAAACTCAA ATTGAAGAGG AACGGAAGAA GTTTCTGGCT 
GTTGATAGGG AAGTGGGGAA ATTGCAAAAA GAAGTTGTAA GTATTCAAAC TTCTCTGGAA 
CAGAAACGAT TAGAGAAGCA TAACTTGCTG CTTGATTGCA AAGTGCAAGA CATTGAGATA 
ATCCTTTTGT CGGGGTCACT GGATGACATC ATTGAAGTGG AGATGGGAAC TGAAGCAGAA 
AGTACCCAGG CAACAATTGA TATCTATGAA AAAGAAGAAG CCTTTGAAAT AGACTACAGC 
TCTCTAAAAG AGGATTTGAA GGCTCTACAG TCTGATCAAG AAATCGAGGC CCACCTTAGG 
CTCTTATTGC AGCAAGTAGC ATCCCAGGAA GATATCTTAC TGAAAACAGC AGCCCCAAAC 
CTACGAGCAC TGGAGAACTT AAAGACTGTC AGAGACAAGT TTCAAGAGTC CACAGATGCT 
TTTGAGGCCA GCAGAAAGGA AGCCAGACTG TGTAGGCAAG AGTTCGAGCA AGTGAAAAAA 
AGGAGATACG ATCTTTTCAC CCAGTGTTTT GAGCATGTCT CAATCTCAAT TGATCAAATC 
TACAAGAAGC TCTGCAGAAA CAACAGCGCC CAAGTGTCAA GTTACATCAA AGAGCAAACT 
CAAGACCAGT TTCAGATGAT AGTCATCTCC CTAAAAGAAG AGTTCTATTC CAGAGCCGAC 
GCGCTGATCG GCATCTATCC TGAGTACGAT GACTGCATGT TCAGCCGAGT TTTGACCCTA 
GATCTTTCTC AGTATCCAGA CACTGAAGGC CAAGAAAGCA GCAAGAGACA CGGAGAGTCC 
CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278430.1
CDS	33..3518
Translation	MAHLELLLVENFKSWRGRQVIGPFRRFTCIIGPNGSGKSNVMDALSFVMGEKIANLRVKNIQELIHGAHIGKPISSSASVKIIYVEESGEEKTFARIIRGGCSEFRFNDNLVSRSVYIAELEKIGIIVKAQNCLVFQGTVESISVKKPKERTQFFEEISTSGELIGEYEEKKRKLQKAEEDAQFNFNKKKNIAAERRQAKLEKEEAERYQSLLEELKMNKIQLQLFQLYHNEKKIHLLNTKLEHVNRDLSVKRESLSHHENIVKARKKEHGMLTRQLQQTEKELKSVETLLNQKRPQYIKAKENTSHHLKKLDVAKKSIKDSEKQCSKQEDDIKALETELADLDAAWRSFEKQIEEEILHKKRDIELEASQLDRYKELKEQVRKKVATMTQQLEKLQWEQKTDEERLAFEKRRHGEVQGNLKQIKEQIEDHKKRIEKLEEYTKTCMDCLKEKKQQEETLVDEIEKTKSRMSEVNEELNLIRSELQNAGIDTHEGKRQQKRAEVLEHLKRLYPDSVFGRLFDLCHPIHKKYQLAVTKVFGRFITAIVVASEKVAKDCIRFLKEERAEPETFLALDYLDIKPINERLRELKGCKMVIDVIKTQFPQLKKVIQFVCGNGLVCETMEEARHIALSGPERQKTVALDGTLFLKSGVISGGSSDLKYKARCWDEKELKNLRDRRSQKIQELKGLMKTLRKETDLKQIQTLIQGTQTRLKYSQNELEMIKKKHLVAFYQEQSQLQSELLNIESQCIMLSEGIKERQRRIKEFQEKIDKVEDDIFQHFCEEIGVENIREFENKHVKRQQEIDQKRLEFEKQKTRLNVQLEYSRSHLKKKLNKINTLKETIQKGSEDIDHLKKAEENCLQTVNELMAKQQQLKDIRVTQNSSAEKVQTQIEEERKKFLAVDREVGKLQKEVVSIQTSLEQKRLEKHNLLLDCKVQDIEIILLSGSLDDIIEVEMGTEAESTQATIDIYEKEEAFEIDYSSLKEDLKALQSDQEIEAHLRLLLQQVASQEDILLKTAAPNLRALENLKTVRDKFQESTDAFEASRKEARLCRQEFEQVKKRRYDLFTQCFEHVSISIDQIYKKLCRNNSAQVSSYIKEQTQDQFQMIVISLKEEFYSRADALIGIYPEYDDCMFSRVLTLDLSQYPDTEGQESSKRHGESR

Target ORF information:

RefSeq Version	NM_001291501.2
Organism	Homo sapiens(human)
Definition	Homo sapiens structural maintenance of chromosomes 1B (SMC1B), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001291501.2

ORF Insert Sequence:

ATGGCCCACC TGGAGCTGCT GCTTGTGGAA AATTTCAAGT CGTGGCGGGG CCGCCAGGTC 
ATTGGCCCCT TCCGGAGGTT CACCTGCATC ATCGGCCCCA ACGGCTCTGG AAAATCTAAT 
GTAATGGATG CACTTAGTTT TGTAATGGGA GAGAAAATAG CTAATTTAAG AGTGAAAAAT 
ATTCAAGAAC TCATTCATGG AGCACATATT GGAAAACCTA TTTCTTCTTC TGCAAGTGTA 
AAAATTATAT ATGTGGAGGA AAGTGGCGAA GAGAAAACAT TTGCAAGGAT TATCCGAGGG 
GGATGCTCAG AATTTCGCTT TAATGATAAT CTTGTGAGTC GTTCTGTTTA CATTGCAGAG 
TTGGAAAAGA TAGGCATAAT AGTCAAAGCA CAAAATTGTT TGGTTTTTCA GGGAACTGTA 
GAGTCAATTT CAGTGAAGAA ACCCAAAGAA AGGACCCAGT TTTTTGAGGA AATCAGCACT 
TCAGGAGAGC TTATAGGAGA ATATGAAGAA AAGAAAAGAA AGTTACAAAA AGCCGAAGAG 
GATGCACAGT TTAACTTTAA TAAGAAAAAA AATATAGCGG CAGAGCGCAG ACAAGCAAAA 
TTAGAGAAGG AAGAGGCAGA ACGTTACCAG AGTCTCCTTG AAGAACTGAA AATGAACAAG 
ATACAACTGC AGCTTTTTCA ACTATACCAT AATGAGAAAA AGATTCATCT CCTGAACACC 
AAGTTAGAGC ATGTGAATAG GGATTTGAGT GTCAAAAGAG AGTCTTTGTC TCATCATGAA 
AACATAGTTA AAGCCAGGAA AAAGGAACAT GGAATGCTAA CTAGACAACT ACAACAAACA 
GAAAAAGAAT TAAAATCGGT TGAAACCCTT TTAAATCAGA AGAGGCCTCA GTACATTAAA 
GCCAAAGAAA ACACTTCTCA CCACCTTAAG AAATTAGATG TGGCTAAGAA ATCAATAAAG 
GACAGCGAAA AACAATGTTC TAAACAGGAA GATGATATAA AAGCCCTGGA GACAGAGCTG 
GCTGATTTAG ATGCTGCATG GAGAAGTTTT GAAAAGCAGA TTGAGGAAGA AATTTTACAT 
AAAAAGCGAG ACATTGAACT GGAAGCCAGT CAGCTGGATC GTTATAAAGA ACTTAAGGAA 
CAAGTAAGAA AGAAAGTAGC TACAATGACT CAACAACTGG AAAAACTGCA GTGGGAACAG 
AAGACAGATG AAGAAAGACT GGCATTTGAA AAGAGGAGGC ATGGAGAAGT TCAGGGAAAT 
CTAAAACAAA TAAAAGAACA AATAGAAGAT CATAAAAAAC GAATAGAGAA GTTAGAGGAG 
TATACAAAGA CATGCATGGA TTGCTTGAAA GAGAAAAAAC AGCAAGAGGA AACCCTAGTG 
GATGAAATTG AAAAAACAAA ATCAAGAATG TCTGAAGTTA ATGAAGAATT GAATCTTATT 
AGAAGTGAAT TGCAGAATGC TGGGATTGAT ACCCATGAGG GAAAACGTCA GCAAAAGAGA 
GCAGAGGTTC TGGAACACCT TAAAAGACTG TACCCAGATT CTGTGTTTGG AAGACTATTT 
GACCTGTGTC ATCCTATTCA TAAGAAATAC CAGCTGGCTG TTACTAAGGT TTTTGGCCGG 
TTCATCACTG CCATTGTTGT AGCCTCTGAA AAGGTAGCAA AAGATTGTAT TCGATTTCTG 
AAGGAGGAAA GAGCTGAACC TGAGACATTC CTCGCTCTAG ATTACCTTGA TATCAAGCCA 
ATCAATGAAA GACTAAGGGA GCTTAAAGGC TGTAAAATGG TGATTGATGT CATAAAGACT 
CAGTTTCCTC AGCTGAAGAA AGTGATTCAG TTTGTGTGTG GAAATGGTCT TGTTTGTGAG 
ACTATGGAAG AAGCAAGGCA TATTGCACTC AGTGGACCTG AAAGACAGAA AACAGTAGCT 
CTTGATGGAA CATTATTTTT AAAATCTGGA GTGATCTCTG GAGGGTCAAG TGACTTAAAA 
TACAAGGCTA GATGCTGGGA TGAGAAAGAG TTAAAGAATC TAAGAGACAG ACGAAGCCAG 
AAAATCCAAG AGCTAAAGGG TTTAATGAAG ACACTCCGCA AAGAAACAGA TTTGAAACAA 
ATACAGACCC TGATACAGGG AACTCAAACA CGACTCAAAT ATTCACAAAA TGAACTAGAG 
ATGATTAAGA AGAAGCACCT TGTTGCTTTT TACCAGGAAC AATCTCAGTT ACAAAGTGAA 
CTACTAAATA TTGAGTCTCA ATGTATTATG TTGAGTGAAG GAATCAAGGA ACGACAACGA 
AGAATTAAAG AATTTCAAGA AAAGATAGAT AAGGTAGAAG ACGATATCTT CCAACACTTC 
TGTGAAGAAA TTGGCGTGGA AAATATTCGT GAATTTGAGA ACAAACATGT TAAACGGCAA 
CAAGAAATTG ATCAAAAAAG ATTAGAATTT GAAAAACAAA AAACTCGGCT TAATGTTCAA 
CTTGAGTATA GTCGCAGTCA CCTTAAGAAG AAACTGAATA AGATCAACAC ATTAAAAGAA 
ACTATCCAGA AAGGTAGTGA AGATATTGAT CACCTAAAGA AGGCTGAAGA AAACTGTCTG 
CAGACAGTGA ATGAACTCAT GGCAAAGCAG CAGCAACTTA AGGACATACG TGTCACTCAG 
AACTCCAGTG CCGAGAAAGT TCAAACTCAA ATTGAAGAGG AACGGAAGAA GTTTCTGGCT 
GTTGATAGGG AAGTGGGGAA ATTGCAAAAA GAAGTTGTAA GTATTCAAAC TTCTCTGGAA 
CAGAAACGAT TAGAGAAGCA TAACTTGCTG CTTGATTGCA AAGTGCAAGA CATTGAGATA 
ATCCTTTTGT CGGGGTCACT GGATGACATC ATTGAAGTGG AGATGGGAAC TGAAGCAGAA 
AGTACCCAGG CAACAATTGA TATCTATGAA AAAGAAGAAG CCTTTGAAAT AGACTACAGC 
TCTCTAAAAG AGGATTTGAA GGCTCTACAG TCTGATCAAG AAATCGAGGC CCACCTTAGG 
CTCTTATTGC AGCAAGTAGC ATCCCAGGAA GATATCTTAC TGAAAACAGC AGCCCCAAAC 
CTACGAGCAC TGGAGAACTT AAAGACTGTC AGAGACAAGT TTCAAGAGTC CACAGATGCT 
TTTGAGGCCA GCAGAAAGGA AGCCAGACTG TGTAGGCAAG AGTTCGAGCA AGTGAAAAAA 
AGGAGATACG ATCTTTTCAC CCAGTGTTTT GAGCATGTCT CAATCTCAAT TGATCAAATC 
TACAAGAAGC TCTGCAGAAA CAACAGCGCC CAAGTGTCAA GTTACATCAA AGAGCAAACT 
CAAGACCAGT TTCAGATGAT AGTCATCTCC CTAAAAGAAG AGTTCTATTC CAGAGCCGAC 
GCGCTGATCG GCATCTATCC TGAGTACGAT GACTGCATGT TCAGCCGAGT TTTGACCCTA 
GATCTTTCTC AGTATCCAGA CACTGAAGGC CAAGAAAGCA GCAAGAGACA CGGAGAGTCC 
CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMC1B ( XM_011530144.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011528446.1 Homo sapiens structural maintenance of chromosomes 1B (SMC1B), transcript variant X1, mRNA.

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CloneID	OHu67073
Clone ID Related Accession (Same CDS sequence)	XM_011530144.2
Accession Version	XM_011530144.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	structural maintenance of chromosomes protein 1B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530144.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCAC TTAGTTTTGT AATGGGAGAG AAAATAGCTA ATTTAAGAGT GAAAAATATT 
CAAGAACTCA TTCATGGAGC ACATATTGGA AAACCTATTT CTTCTTCTGC AAGTGTAAAA 
ATTATATATG TGGAGGAAAG TGGCGAAGAG AAAACATTTG CAAGGATTAT CCGAGGGGGA 
TGCTCAGAAT TTCGCTTTAA TGATAATCTT GTGAGTCGTT CTGTTTACAT TGCAGAGTTG 
GAAAAGATAG GCATAATAGT CAAAGCACAA AATTGTTTGG TTTTTCAGGG AACTGTAGAG 
TCAATTTCAG TGAAGAAACC CAAAGAAAGG ACCCAGTTTT TTGAGGAAAT CAGCACTTCA 
GGAGAGCTTA TAGGAGAATA TGAAGAAAAG AAAAGAAAGT TACAAAAAGC CGAAGAGGAT 
GCACAGTTTA ACTTTAATAA GAAAAAAAAT ATAGCGGCAG AGCGCAGACA AGCAAAATTA 
GAGAAGGAAG AGGCAGAACG TTACCAGAGT CTCCTTGAAG AACTGAAAAT GAACAAGATA 
CAACTGCAGC TTTTTCAACT ATACCATAAT GAGAAAAAGA TTCATCTCCT GAACACCAAG 
TTAGAGCATG TGAATAGGGA TTTGAGTGTC AAAAGAGAGT CTTTGTCTCA TCATGAAAAC 
ATAGTTAAAG CCAGGAAAAA GGAACATGGA ATGCTAACTA GACAACTACA ACAAACAGAA 
AAAGAATTAA AATCGGTTGA AACCCTTTTA AATCAGAAGA GGCCTCAGTA CATTAAAGCC 
AAAGAAAACA CTTCTCACCA CCTTAAGAAA TTAGATGTGG CTAAGAAATC AATAAAGGAC 
AGCGAAAAAC AATGTTCTAA ACAGGAAGAT GATATAAAAG CCCTGGAGAC AGAGCTGGCT 
GATTTAGATG CTGCATGGAG AAGTTTTGAA AAGCAGATTG AGGAAGAAAT TTTACATAAA 
AAGCGAGACA TTGAACTGGA AGCCAGTCAG CTGGATCGTT ATAAAGAACT TAAGGAACAA 
GTAAGAAAGA AAGTAGCTAC AATGACTCAA CAACTGGAAA AACTGCAGTG GGAACAGAAG 
ACAGATGAAG AAAGACTGGC ATTTGAAAAG AGGAGGCATG GAGAAGTTCA GGGAAATCTA 
AAACAAATAA AAGAACAAAT AGAAGATCAT AAAAAACGAA TAGAGAAGTT AGAGGAGTAT 
ACAAAGACAT GCATGGATTG CTTGAAAGAG AAAAAACAGC AAGAGGAAAC CCTAGTGGAT 
GAAATTGAAA AAACAAAATC AAGAATGTCT GAAGTTAATG AAGAATTGAA TCTTATTAGA 
AGTGAATTGC AGAATGCTGG GATTGATACC CATGAGGGAA AACGTCAGCA AAAGAGAGCA 
GAGGTTCTGG AACACCTTAA AAGACTGTAC CCAGATTCTG TGTTTGGAAG ACTATTTGAC 
CTGTGTCATC CTATTCATAA GAAATACCAG CTGGCTGTTA CTAAGGTTTT TGGCCGGTTC 
ATCACTGCCA TTGTTGTAGC CTCTGAAAAG GTAGCAAAAG ATTGTATTCG ATTTCTGAAG 
GAGGAAAGAG CTGAACCTGA GACATTCCTC GCTCTAGATT ACCTTGATAT CAAGCCAATC 
AATGAAAGAC TAAGGGAGCT TAAAGGCTGT AAAATGGTGA TTGATGTCAT AAAGACTCAG 
TTTCCTCAGC TGAAGAAAGT GATTCAGTTT GTGTGTGGAA ATGGTCTTGT TTGTGAGACT 
ATGGAAGAAG CAAGGCATAT TGCACTCAGT GGACCTGAAA GACAGAAAAC AGTAGCTCTT 
GATGGAACAT TATTTTTAAA ATCTGGAGTG ATCTCTGGAG GGTCAAGTGA CTTAAAATAC 
AAGGCTAGAT GCTGGGATGA GAAAGAGTTA AAGAATCTAA GAGACAGACG AAGCCAGAAA 
ATCCAAGAGC TAAAGGGTTT AATGAAGACA CTCCGCAAAG AAACAGATTT GAAACAAATA 
CAGACCCTGA TACAGGGAAC TCAAACACGA CTCAAATATT CACAAAATGA ACTAGAGATG 
ATTAAGAAGA AGCACCTTGT TGCTTTTTAC CAGGAACAAT CTCAGTTACA AAGTGAACTA 
CTAAATATTG AGTCTCAATG TATTATGTTG AGTGAAGGAA TCAAGGAACG ACAACGAAGA 
ATTAAAGAAT TTCAAGAAAA GATAGATAAG GTAGAAGACG ATATCTTCCA ACACTTCTGT 
GAAGAAATTG GCGTGGAAAA TATTCGTGAA TTTGAGAACA AACATGTTAA ACGGCAACAA 
GAAATTGATC AAAAAAGATT AGAATTTGAA AAACAAAAAA CTCGGCTTAA TGTTCAACTT 
GAGTATAGTC GCAGTCACCT TAAGAAGAAA CTGAATAAGA TCAACACATT AAAAGAAACT 
ATCCAGAAAG GTAGTGAAGA TATTGATCAC CTAAAGAAGG CTGAAGAAAA CTGTCTGCAG 
ACAGTGAATG AACTCATGGC AAAGCAGCAG CAACTTAAGG ACATACGTGT CACTCAGAAC 
TCCAGTGCCG AGAAAGTTCA AACTCAAATT GAAGAGGAAC GGAAGAAGTT TCTGGCTGTT 
GATAGGGAAG TGGGGAAATT GCAAAAAGAA GTTGTAAGTA TTCAAACTTC TCTGGAACAG 
AAACGATTAG AGAAGCATAA CTTGCTGCTT GATTGCAAAG TGCAAGACAT TGAGATAATC 
CTTTTGTCGG GGTCACTGGA TGACATCATT GAAGTGGAGA TGGGAACTGA AGCAGAAAGT 
ACCCAGGCAA CAATTGATAT CTATGAAAAA GAAGAAGCCT TTGAAATAGA CTACAGCTCT 
CTAAAAGAGG ATTTGAAGGC TCTACAGTCT GATCAAGAAA TCGAGGCCCA CCTTAGGCTC 
TTATTGCAGC AAGTAGCATC CCAGGAAGAT ATCTTACTGA AAACAGCAGC CCCAAACCTA 
CGAGCACTGG AGAACTTAAA GACTGTCAGA GACAAGTTTC AAGAGTCCAC AGATGCTTTT 
GAGGCCAGCA GAAAGGAAGC CAGACTGTGT AGGCAAGAGT TCGAGCAAGT GAAAAAAAGG 
AGATACGATC TTTTCACCCA GTGTTTTGAG CATGTCTCAA TCTCAATTGA TCAAATCTAC 
AAGAAGCTCT GCAGAAACAA CAGCGCCCAA GCATTTCTTA GCCCAGAGAA CCCTGAAGAA 
CCTTACTTGG AGGGAATTAG CTATAACTGT GTGGCCCCAG GCAAACGGTT TATGCCAATG 
GACAATTTGT CAGGGGGAGA AAAGTGTGTG GCAGCCTTGG CTCTCCTGTT TGCTGTGCAC 
AGTTTTCGTC CTGCCCCATT CTTTGTTTTA GATGAAGTGG ATGCAGCCCT AGACAATACT 
AACATAGGCA AAGTGTCAAG TTACATCAAA GAGCAAACTC AAGACCAGTT TCAGATGATA 
GTCATCTCCC TAAAAGAAGA GTTCTATTCC AGAGCCGACG CGCTGATCGG CATCTATCCT 
GAGTACGATG ACTGCATGTT CAGCCGAGTT TTGACCCTAG ATCTTTCTCA GTATCCAGAC 
ACTGAAGGCC AAGAAAGCAG CAAGAGACAC GGAGAGTCCC GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011528446.1
CDS	37..3621
Translation	MDALSFVMGEKIANLRVKNIQELIHGAHIGKPISSSASVKIIYVEESGEEKTFARIIRGGCSEFRFNDNLVSRSVYIAELEKIGIIVKAQNCLVFQGTVESISVKKPKERTQFFEEISTSGELIGEYEEKKRKLQKAEEDAQFNFNKKKNIAAERRQAKLEKEEAERYQSLLEELKMNKIQLQLFQLYHNEKKIHLLNTKLEHVNRDLSVKRESLSHHENIVKARKKEHGMLTRQLQQTEKELKSVETLLNQKRPQYIKAKENTSHHLKKLDVAKKSIKDSEKQCSKQEDDIKALETELADLDAAWRSFEKQIEEEILHKKRDIELEASQLDRYKELKEQVRKKVATMTQQLEKLQWEQKTDEERLAFEKRRHGEVQGNLKQIKEQIEDHKKRIEKLEEYTKTCMDCLKEKKQQEETLVDEIEKTKSRMSEVNEELNLIRSELQNAGIDTHEGKRQQKRAEVLEHLKRLYPDSVFGRLFDLCHPIHKKYQLAVTKVFGRFITAIVVASEKVAKDCIRFLKEERAEPETFLALDYLDIKPINERLRELKGCKMVIDVIKTQFPQLKKVIQFVCGNGLVCETMEEARHIALSGPERQKTVALDGTLFLKSGVISGGSSDLKYKARCWDEKELKNLRDRRSQKIQELKGLMKTLRKETDLKQIQTLIQGTQTRLKYSQNELEMIKKKHLVAFYQEQSQLQSELLNIESQCIMLSEGIKERQRRIKEFQEKIDKVEDDIFQHFCEEIGVENIREFENKHVKRQQEIDQKRLEFEKQKTRLNVQLEYSRSHLKKKLNKINTLKETIQKGSEDIDHLKKAEENCLQTVNELMAKQQQLKDIRVTQNSSAEKVQTQIEEERKKFLAVDREVGKLQKEVVSIQTSLEQKRLEKHNLLLDCKVQDIEIILLSGSLDDIIEVEMGTEAESTQATIDIYEKEEAFEIDYSSLKEDLKALQSDQEIEAHLRLLLQQVASQEDILLKTAAPNLRALENLKTVRDKFQESTDAFEASRKEARLCRQEFEQVKKRRYDLFTQCFEHVSISIDQIYKKLCRNNSAQAFLSPENPEEPYLEGISYNCVAPGKRFMPMDNLSGGEKCVAALALLFAVHSFRPAPFFVLDEVDAALDNTNIGKVSSYIKEQTQDQFQMIVISLKEEFYSRADALIGIYPEYDDCMFSRVLTLDLSQYPDTEGQESSKRHGESR

Target ORF information:

RefSeq Version	XM_011530144.2
Organism	Homo sapiens(human)
Definition	Homo sapiens structural maintenance of chromosomes 1B (SMC1B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011530144.2

ORF Insert Sequence:

ATGGATGCAC TTAGTTTTGT AATGGGAGAG AAAATAGCTA ATTTAAGAGT GAAAAATATT 
CAAGAACTCA TTCATGGAGC ACATATTGGA AAACCTATTT CTTCTTCTGC AAGTGTAAAA 
ATTATATATG TGGAGGAAAG TGGCGAAGAG AAAACATTTG CAAGGATTAT CCGAGGGGGA 
TGCTCAGAAT TTCGCTTTAA TGATAATCTT GTGAGTCGTT CTGTTTACAT TGCAGAGTTG 
GAAAAGATAG GCATAATAGT CAAAGCACAA AATTGTTTGG TTTTTCAGGG AACTGTAGAG 
TCAATTTCAG TGAAGAAACC CAAAGAAAGG ACCCAGTTTT TTGAGGAAAT CAGCACTTCA 
GGAGAGCTTA TAGGAGAATA TGAAGAAAAG AAAAGAAAGT TACAAAAAGC CGAAGAGGAT 
GCACAGTTTA ACTTTAATAA GAAAAAAAAT ATAGCGGCAG AGCGCAGACA AGCAAAATTA 
GAGAAGGAAG AGGCAGAACG TTACCAGAGT CTCCTTGAAG AACTGAAAAT GAACAAGATA 
CAACTGCAGC TTTTTCAACT ATACCATAAT GAGAAAAAGA TTCATCTCCT GAACACCAAG 
TTAGAGCATG TGAATAGGGA TTTGAGTGTC AAAAGAGAGT CTTTGTCTCA TCATGAAAAC 
ATAGTTAAAG CCAGGAAAAA GGAACATGGA ATGCTAACTA GACAACTACA ACAAACAGAA 
AAAGAATTAA AATCGGTTGA AACCCTTTTA AATCAGAAGA GGCCTCAGTA CATTAAAGCC 
AAAGAAAACA CTTCTCACCA CCTTAAGAAA TTAGATGTGG CTAAGAAATC AATAAAGGAC 
AGCGAAAAAC AATGTTCTAA ACAGGAAGAT GATATAAAAG CCCTGGAGAC AGAGCTGGCT 
GATTTAGATG CTGCATGGAG AAGTTTTGAA AAGCAGATTG AGGAAGAAAT TTTACATAAA 
AAGCGAGACA TTGAACTGGA AGCCAGTCAG CTGGATCGTT ATAAAGAACT TAAGGAACAA 
GTAAGAAAGA AAGTAGCTAC AATGACTCAA CAACTGGAAA AACTGCAGTG GGAACAGAAG 
ACAGATGAAG AAAGACTGGC ATTTGAAAAG AGGAGGCATG GAGAAGTTCA GGGAAATCTA 
AAACAAATAA AAGAACAAAT AGAAGATCAT AAAAAACGAA TAGAGAAGTT AGAGGAGTAT 
ACAAAGACAT GCATGGATTG CTTGAAAGAG AAAAAACAGC AAGAGGAAAC CCTAGTGGAT 
GAAATTGAAA AAACAAAATC AAGAATGTCT GAAGTTAATG AAGAATTGAA TCTTATTAGA 
AGTGAATTGC AGAATGCTGG GATTGATACC CATGAGGGAA AACGTCAGCA AAAGAGAGCA 
GAGGTTCTGG AACACCTTAA AAGACTGTAC CCAGATTCTG TGTTTGGAAG ACTATTTGAC 
CTGTGTCATC CTATTCATAA GAAATACCAG CTGGCTGTTA CTAAGGTTTT TGGCCGGTTC 
ATCACTGCCA TTGTTGTAGC CTCTGAAAAG GTAGCAAAAG ATTGTATTCG ATTTCTGAAG 
GAGGAAAGAG CTGAACCTGA GACATTCCTC GCTCTAGATT ACCTTGATAT CAAGCCAATC 
AATGAAAGAC TAAGGGAGCT TAAAGGCTGT AAAATGGTGA TTGATGTCAT AAAGACTCAG 
TTTCCTCAGC TGAAGAAAGT GATTCAGTTT GTGTGTGGAA ATGGTCTTGT TTGTGAGACT 
ATGGAAGAAG CAAGGCATAT TGCACTCAGT GGACCTGAAA GACAGAAAAC AGTAGCTCTT 
GATGGAACAT TATTTTTAAA ATCTGGAGTG ATCTCTGGAG GGTCAAGTGA CTTAAAATAC 
AAGGCTAGAT GCTGGGATGA GAAAGAGTTA AAGAATCTAA GAGACAGACG AAGCCAGAAA 
ATCCAAGAGC TAAAGGGTTT AATGAAGACA CTCCGCAAAG AAACAGATTT GAAACAAATA 
CAGACCCTGA TACAGGGAAC TCAAACACGA CTCAAATATT CACAAAATGA ACTAGAGATG 
ATTAAGAAGA AGCACCTTGT TGCTTTTTAC CAGGAACAAT CTCAGTTACA AAGTGAACTA 
CTAAATATTG AGTCTCAATG TATTATGTTG AGTGAAGGAA TCAAGGAACG ACAACGAAGA 
ATTAAAGAAT TTCAAGAAAA GATAGATAAG GTAGAAGACG ATATCTTCCA ACACTTCTGT 
GAAGAAATTG GCGTGGAAAA TATTCGTGAA TTTGAGAACA AACATGTTAA ACGGCAACAA 
GAAATTGATC AAAAAAGATT AGAATTTGAA AAACAAAAAA CTCGGCTTAA TGTTCAACTT 
GAGTATAGTC GCAGTCACCT TAAGAAGAAA CTGAATAAGA TCAACACATT AAAAGAAACT 
ATCCAGAAAG GTAGTGAAGA TATTGATCAC CTAAAGAAGG CTGAAGAAAA CTGTCTGCAG 
ACAGTGAATG AACTCATGGC AAAGCAGCAG CAACTTAAGG ACATACGTGT CACTCAGAAC 
TCCAGTGCCG AGAAAGTTCA AACTCAAATT GAAGAGGAAC GGAAGAAGTT TCTGGCTGTT 
GATAGGGAAG TGGGGAAATT GCAAAAAGAA GTTGTAAGTA TTCAAACTTC TCTGGAACAG 
AAACGATTAG AGAAGCATAA CTTGCTGCTT GATTGCAAAG TGCAAGACAT TGAGATAATC 
CTTTTGTCGG GGTCACTGGA TGACATCATT GAAGTGGAGA TGGGAACTGA AGCAGAAAGT 
ACCCAGGCAA CAATTGATAT CTATGAAAAA GAAGAAGCCT TTGAAATAGA CTACAGCTCT 
CTAAAAGAGG ATTTGAAGGC TCTACAGTCT GATCAAGAAA TCGAGGCCCA CCTTAGGCTC 
TTATTGCAGC AAGTAGCATC CCAGGAAGAT ATCTTACTGA AAACAGCAGC CCCAAACCTA 
CGAGCACTGG AGAACTTAAA GACTGTCAGA GACAAGTTTC AAGAGTCCAC AGATGCTTTT 
GAGGCCAGCA GAAAGGAAGC CAGACTGTGT AGGCAAGAGT TCGAGCAAGT GAAAAAAAGG 
AGATACGATC TTTTCACCCA GTGTTTTGAG CATGTCTCAA TCTCAATTGA TCAAATCTAC 
AAGAAGCTCT GCAGAAACAA CAGCGCCCAA GCATTTCTTA GCCCAGAGAA CCCTGAAGAA 
CCTTACTTGG AGGGAATTAG CTATAACTGT GTGGCCCCAG GCAAACGGTT TATGCCAATG 
GACAATTTGT CAGGGGGAGA AAAGTGTGTG GCAGCCTTGG CTCTCCTGTT TGCTGTGCAC 
AGTTTTCGTC CTGCCCCATT CTTTGTTTTA GATGAAGTGG ATGCAGCCCT AGACAATACT 
AACATAGGCA AAGTGTCAAG TTACATCAAA GAGCAAACTC AAGACCAGTT TCAGATGATA 
GTCATCTCCC TAAAAGAAGA GTTCTATTCC AGAGCCGACG CGCTGATCGG CATCTATCCT 
GAGTACGATG ACTGCATGTT CAGCCGAGTT TTGACCCTAG ATCTTTCTCA GTATCCAGAC 
ACTGAAGGCC AAGAAAGCAG CAAGAGACAC GGAGAGTCCC GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMC1B ( XM_011530145.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011528447.1 Homo sapiens structural maintenance of chromosomes 1B (SMC1B), transcript variant X3, mRNA.

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CloneID	OHu67074
Clone ID Related Accession (Same CDS sequence)	XM_011530145.2
Accession Version	XM_011530145.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	structural maintenance of chromosomes protein 1B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530145.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCACC TGGAGCTGCT GCTTGTGGAA AATTTCAAGT CGTGGCGGGG CCGCCAGGTC 
ATTGGCCCCT TCCGGAGGTT CACCTGCATC ATCGGCCCCA ACGGCTCTGG AAAATCTAAT 
GTAATGGATG CACTTAGTTT TGTAATGGGA GAGAAAATAG CTAATTTAAG AGTGAAAAAT 
ATTCAAGAAC TCATTCATGG AGCACATATT GGAAAACCTA TTTCTTCTTC TGCAAGTGTA 
AAAATTATAT ATGTGGAGGA AAGTGGCGAA GAGAAAACAT TTGCAAGGAT TATCCGAGGG 
GGATGCTCAG AATTTCGCTT TAATGATAAT CTTGTGAGTC GTTCTGTTTA CATTGCAGAG 
TTGGAAAAGA TAGGCATAAT AGTCAAAGCA CAAAATTGTT TGGTTTTTCA GGGAACTGTA 
GAGTCAATTT CAGTGAAGAA ACCCAAAGAA AGGACCCAGT TTTTTGAGGA AATCAGCACT 
TCAGGAGAGC TTATAGGAGA ATATGAAGAA AAGAAAAGAA AGTTACAAAA AGCCGAAGAG 
GATGCACAGT TTAACTTTAA TAAGAAAAAA AATATAGCGG CAGAGCGCAG ACAAGCAAAA 
TTAGAGAAGG AAGAGGCAGA ACGTTACCAG AGTCTCCTTG AAGAACTGAA AATGAACAAG 
ATACAACTGC AGCTTTTTCA ACTATACCAT AATGAGAAAA AGATTCATCT CCTGAACACC 
AAGTTAGAGC ATGTGAATAG GGATTTGAGT GTCAAAAGAG AGTCTTTGTC TCATCATGAA 
AACATAGTTA AAGCCAGGAA AAAGGAACAT GGAATGCTAA CTAGACAACT ACAACAAACA 
GAAAAAGAAT TAAAATCGGT TGAAACCCTT TTAAATCAGA AGAGGCCTCA GTACATTAAA 
GCCAAAGAAA ACACTTCTCA CCACCTTAAG AAATTAGATG TGGCTAAGAA ATCAATAAAG 
GACAGCGAAA AACAATGTTC TAAACAGGAA GATGATATAA AAGCCCTGGA GACAGAGCTG 
GCTGATTTAG ATGCTGCATG GAGAAGTTTT GAAAAGCAGA TTGAGGAAGA AATTTTACAT 
AAAAAGCGAG ACATTGAACT GGAAGCCAGT CAGCTGGATC GTTATAAAGA ACTTAAGGAA 
CAAGTAAGAA AGAAAGTAGC TACAATGACT CAACAACTGG AAAAACTGCA GTGGGAACAG 
AAGACAGATG AAGAAAGACT GGCATTTGAA AAGAGGAGGC ATGGAGAAGT TCAGGGAAAT 
CTAAAACAAA TAAAAGAACA AATAGAAGAT CATAAAAAAC GAATAGAGAA GTTAGAGGAG 
TATACAAAGA CATGCATGGA TTGCTTGAAA GAGAAAAAAC AGCAAGAGGA AACCCTAGTG 
GATGAAATTG AAAAAACAAA ATCAAGAATG TCTGAAGTTA ATGAAGAATT GAATCTTATT 
AGAAGTGAAT TGCAGAATGC TGGGATTGAT ACCCATGAGG GAAAACGTCA GCAAAAGAGA 
GCAGAGGTTC TGGAACACCT TAAAAGACTG TACCCAGATT CTGTGTTTGG AAGACTATTT 
GACCTGTGTC ATCCTATTCA TAAGAAATAC CAGCTGGCTG TTACTAAGGT TTTTGGCCGG 
TTCATCACTG CCATTGTTGT AGCCTCTGAA AAGGTAGCAA AAGATTGTAT TCGATTTCTG 
AAGGAGGAAA GAGCTGAACC TGAGACATTC CTCGCTCTAG ATTACCTTGA TATCAAGCCA 
ATCAATGAAA GACTAAGGGA GCTTAAAGGC TGTAAAATGG TGATTGATGT CATAAAGACT 
CAGTTTCCTC AGCTGAAGAA AGTGATTCAG TTTGTGTGTG GAAATGGTCT TGTTTGTGAG 
ACTATGGAAG AAGCAAGGCA TATTGCACTC AGTGGACCTG AAAGACAGAA AACAGTAGCT 
CTTGATGGAA CATTATTTTT AAAATCTGGA GTGATCTCTG GAGGGTCAAG TGACTTAAAA 
TACAAGGCTA GATGCTGGGA TGAGAAAGAG TTAAAGAATC TAAGAGACAG ACGAAGCCAG 
AAAATCCAAG AGCTAAAGGG TTTAATGAAG ACACTCCGCA AAGAAACAGA TTTGAAACAA 
ATACAGACCC TGATACAGGG AACTCAAACA CGACTCAAAT ATTCACAAAA TGAACTAGAG 
ATGATTAAGA AGAAGCACCT TGTTGCTTTT TACCAGGAAC AATCTCAGTT ACAAAGTGAA 
CTACTAAATA TTGAGTCTCA ATGTATTATG TTGAGTGAAG GAATCAAGGA ACGACAACGA 
AGAATTAAAG AATTTCAAGA AAAGATAGAT AAGGTAGAAG ACGATATCTT CCAACACTTC 
TGTGAAGAAA TTGGCGTGGA AAATATTCGT GAATTTGAGA ACAAACATGT TAAACGGCAA 
CAAGAAATTG ATCAAAAAAG ATTAGAATTT GAAAAACAAA AAACTCGGCT TAATGTTCAA 
CTTGAGTATA GTCGCAGTCA CCTTAAGAAG AAACTGAATA AGATCAACAC ATTAAAAGAA 
ACTATCCAGA AAGGTAGTGA AGATATTGAT CACCTAAAGA AGGCTGAAGA AAACTGTCTG 
CAGACAGTGA ATGAACTCAT GGCAAAGCAG CAGCAACTTA AGGACATACG TGTCACTCAG 
AACTCCAGTG CCGAGAAAGT TCAAACTCAA ATTGAAGAGG AACGGAAGAA GTTTCTGGCT 
GTTGATAGGG AAGTGGGGAA ATTGCAAAAA GAAGTTGTAA GTATTCAAAC TTCTCTGGAA 
CAGAAACGAT TAGAGAAGCA TAACTTGCTG CTTGATTGCA AAGTGCAAGA CATTGAGATA 
ATCCTTTTGT CGGGGTCACT GGATGACATC ATTGAAGTGG AGATGGGAAC TGAAGCAGAA 
AGTACCCAGG CAACAATTGA TATCTATGAA AAAGAAGAAG CCTTTGAAAT AGACTACAGC 
TCTCTAAAAG AGGATTTGAA GGCTCTACAG TCTGATCAAG AAATCGAGGC CCACCTTAGG 
CTCTTATTGC AGCAAGTAGC ATCCCAGGAA GATATCTTAC TGAAAACAGC AGCCCCAAAC 
CTACGAGCAC TGGAGAACTT AAAGACTGTC AGAGACAAGT TTCAAGAGTC CACAGATGCT 
TTTGAGGCCA GCAGAAAGGA AGCCAGACTG TGTAGGCAAG AGTTCGAGCA AGTGAAAAAA 
AGGAGATACG ATCTTTTCAC CCAGTGTTTT GAGCATGTCT CAATCTCAAT TGATCAAATC 
TACAAGAAGC TCTGCAGAAA CAACAGCGCC CAATTTTCGT CCTGCCCCAT TCTTTGTTTT 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011528447.1
CDS	105..3410
Translation	MAHLELLLVENFKSWRGRQVIGPFRRFTCIIGPNGSGKSNVMDALSFVMGEKIANLRVKNIQELIHGAHIGKPISSSASVKIIYVEESGEEKTFARIIRGGCSEFRFNDNLVSRSVYIAELEKIGIIVKAQNCLVFQGTVESISVKKPKERTQFFEEISTSGELIGEYEEKKRKLQKAEEDAQFNFNKKKNIAAERRQAKLEKEEAERYQSLLEELKMNKIQLQLFQLYHNEKKIHLLNTKLEHVNRDLSVKRESLSHHENIVKARKKEHGMLTRQLQQTEKELKSVETLLNQKRPQYIKAKENTSHHLKKLDVAKKSIKDSEKQCSKQEDDIKALETELADLDAAWRSFEKQIEEEILHKKRDIELEASQLDRYKELKEQVRKKVATMTQQLEKLQWEQKTDEERLAFEKRRHGEVQGNLKQIKEQIEDHKKRIEKLEEYTKTCMDCLKEKKQQEETLVDEIEKTKSRMSEVNEELNLIRSELQNAGIDTHEGKRQQKRAEVLEHLKRLYPDSVFGRLFDLCHPIHKKYQLAVTKVFGRFITAIVVASEKVAKDCIRFLKEERAEPETFLALDYLDIKPINERLRELKGCKMVIDVIKTQFPQLKKVIQFVCGNGLVCETMEEARHIALSGPERQKTVALDGTLFLKSGVISGGSSDLKYKARCWDEKELKNLRDRRSQKIQELKGLMKTLRKETDLKQIQTLIQGTQTRLKYSQNELEMIKKKHLVAFYQEQSQLQSELLNIESQCIMLSEGIKERQRRIKEFQEKIDKVEDDIFQHFCEEIGVENIREFENKHVKRQQEIDQKRLEFEKQKTRLNVQLEYSRSHLKKKLNKINTLKETIQKGSEDIDHLKKAEENCLQTVNELMAKQQQLKDIRVTQNSSAEKVQTQIEEERKKFLAVDREVGKLQKEVVSIQTSLEQKRLEKHNLLLDCKVQDIEIILLSGSLDDIIEVEMGTEAESTQATIDIYEKEEAFEIDYSSLKEDLKALQSDQEIEAHLRLLLQQVASQEDILLKTAAPNLRALENLKTVRDKFQESTDAFEASRKEARLCRQEFEQVKKRRYDLFTQCFEHVSISIDQIYKKLCRNNSAQFSSCPILCFR

Target ORF information:

RefSeq Version	XM_011530145.2
Organism	Homo sapiens(human)
Definition	Homo sapiens structural maintenance of chromosomes 1B (SMC1B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011530145.2

ORF Insert Sequence:

ATGGCCCACC TGGAGCTGCT GCTTGTGGAA AATTTCAAGT CGTGGCGGGG CCGCCAGGTC 
ATTGGCCCCT TCCGGAGGTT CACCTGCATC ATCGGCCCCA ACGGCTCTGG AAAATCTAAT 
GTAATGGATG CACTTAGTTT TGTAATGGGA GAGAAAATAG CTAATTTAAG AGTGAAAAAT 
ATTCAAGAAC TCATTCATGG AGCACATATT GGAAAACCTA TTTCTTCTTC TGCAAGTGTA 
AAAATTATAT ATGTGGAGGA AAGTGGCGAA GAGAAAACAT TTGCAAGGAT TATCCGAGGG 
GGATGCTCAG AATTTCGCTT TAATGATAAT CTTGTGAGTC GTTCTGTTTA CATTGCAGAG 
TTGGAAAAGA TAGGCATAAT AGTCAAAGCA CAAAATTGTT TGGTTTTTCA GGGAACTGTA 
GAGTCAATTT CAGTGAAGAA ACCCAAAGAA AGGACCCAGT TTTTTGAGGA AATCAGCACT 
TCAGGAGAGC TTATAGGAGA ATATGAAGAA AAGAAAAGAA AGTTACAAAA AGCCGAAGAG 
GATGCACAGT TTAACTTTAA TAAGAAAAAA AATATAGCGG CAGAGCGCAG ACAAGCAAAA 
TTAGAGAAGG AAGAGGCAGA ACGTTACCAG AGTCTCCTTG AAGAACTGAA AATGAACAAG 
ATACAACTGC AGCTTTTTCA ACTATACCAT AATGAGAAAA AGATTCATCT CCTGAACACC 
AAGTTAGAGC ATGTGAATAG GGATTTGAGT GTCAAAAGAG AGTCTTTGTC TCATCATGAA 
AACATAGTTA AAGCCAGGAA AAAGGAACAT GGAATGCTAA CTAGACAACT ACAACAAACA 
GAAAAAGAAT TAAAATCGGT TGAAACCCTT TTAAATCAGA AGAGGCCTCA GTACATTAAA 
GCCAAAGAAA ACACTTCTCA CCACCTTAAG AAATTAGATG TGGCTAAGAA ATCAATAAAG 
GACAGCGAAA AACAATGTTC TAAACAGGAA GATGATATAA AAGCCCTGGA GACAGAGCTG 
GCTGATTTAG ATGCTGCATG GAGAAGTTTT GAAAAGCAGA TTGAGGAAGA AATTTTACAT 
AAAAAGCGAG ACATTGAACT GGAAGCCAGT CAGCTGGATC GTTATAAAGA ACTTAAGGAA 
CAAGTAAGAA AGAAAGTAGC TACAATGACT CAACAACTGG AAAAACTGCA GTGGGAACAG 
AAGACAGATG AAGAAAGACT GGCATTTGAA AAGAGGAGGC ATGGAGAAGT TCAGGGAAAT 
CTAAAACAAA TAAAAGAACA AATAGAAGAT CATAAAAAAC GAATAGAGAA GTTAGAGGAG 
TATACAAAGA CATGCATGGA TTGCTTGAAA GAGAAAAAAC AGCAAGAGGA AACCCTAGTG 
GATGAAATTG AAAAAACAAA ATCAAGAATG TCTGAAGTTA ATGAAGAATT GAATCTTATT 
AGAAGTGAAT TGCAGAATGC TGGGATTGAT ACCCATGAGG GAAAACGTCA GCAAAAGAGA 
GCAGAGGTTC TGGAACACCT TAAAAGACTG TACCCAGATT CTGTGTTTGG AAGACTATTT 
GACCTGTGTC ATCCTATTCA TAAGAAATAC CAGCTGGCTG TTACTAAGGT TTTTGGCCGG 
TTCATCACTG CCATTGTTGT AGCCTCTGAA AAGGTAGCAA AAGATTGTAT TCGATTTCTG 
AAGGAGGAAA GAGCTGAACC TGAGACATTC CTCGCTCTAG ATTACCTTGA TATCAAGCCA 
ATCAATGAAA GACTAAGGGA GCTTAAAGGC TGTAAAATGG TGATTGATGT CATAAAGACT 
CAGTTTCCTC AGCTGAAGAA AGTGATTCAG TTTGTGTGTG GAAATGGTCT TGTTTGTGAG 
ACTATGGAAG AAGCAAGGCA TATTGCACTC AGTGGACCTG AAAGACAGAA AACAGTAGCT 
CTTGATGGAA CATTATTTTT AAAATCTGGA GTGATCTCTG GAGGGTCAAG TGACTTAAAA 
TACAAGGCTA GATGCTGGGA TGAGAAAGAG TTAAAGAATC TAAGAGACAG ACGAAGCCAG 
AAAATCCAAG AGCTAAAGGG TTTAATGAAG ACACTCCGCA AAGAAACAGA TTTGAAACAA 
ATACAGACCC TGATACAGGG AACTCAAACA CGACTCAAAT ATTCACAAAA TGAACTAGAG 
ATGATTAAGA AGAAGCACCT TGTTGCTTTT TACCAGGAAC AATCTCAGTT ACAAAGTGAA 
CTACTAAATA TTGAGTCTCA ATGTATTATG TTGAGTGAAG GAATCAAGGA ACGACAACGA 
AGAATTAAAG AATTTCAAGA AAAGATAGAT AAGGTAGAAG ACGATATCTT CCAACACTTC 
TGTGAAGAAA TTGGCGTGGA AAATATTCGT GAATTTGAGA ACAAACATGT TAAACGGCAA 
CAAGAAATTG ATCAAAAAAG ATTAGAATTT GAAAAACAAA AAACTCGGCT TAATGTTCAA 
CTTGAGTATA GTCGCAGTCA CCTTAAGAAG AAACTGAATA AGATCAACAC ATTAAAAGAA 
ACTATCCAGA AAGGTAGTGA AGATATTGAT CACCTAAAGA AGGCTGAAGA AAACTGTCTG 
CAGACAGTGA ATGAACTCAT GGCAAAGCAG CAGCAACTTA AGGACATACG TGTCACTCAG 
AACTCCAGTG CCGAGAAAGT TCAAACTCAA ATTGAAGAGG AACGGAAGAA GTTTCTGGCT 
GTTGATAGGG AAGTGGGGAA ATTGCAAAAA GAAGTTGTAA GTATTCAAAC TTCTCTGGAA 
CAGAAACGAT TAGAGAAGCA TAACTTGCTG CTTGATTGCA AAGTGCAAGA CATTGAGATA 
ATCCTTTTGT CGGGGTCACT GGATGACATC ATTGAAGTGG AGATGGGAAC TGAAGCAGAA 
AGTACCCAGG CAACAATTGA TATCTATGAA AAAGAAGAAG CCTTTGAAAT AGACTACAGC 
TCTCTAAAAG AGGATTTGAA GGCTCTACAG TCTGATCAAG AAATCGAGGC CCACCTTAGG 
CTCTTATTGC AGCAAGTAGC ATCCCAGGAA GATATCTTAC TGAAAACAGC AGCCCCAAAC 
CTACGAGCAC TGGAGAACTT AAAGACTGTC AGAGACAAGT TTCAAGAGTC CACAGATGCT 
TTTGAGGCCA GCAGAAAGGA AGCCAGACTG TGTAGGCAAG AGTTCGAGCA AGTGAAAAAA 
AGGAGATACG ATCTTTTCAC CCAGTGTTTT GAGCATGTCT CAATCTCAAT TGATCAAATC 
TACAAGAAGC TCTGCAGAAA CAACAGCGCC CAATTTTCGT CCTGCCCCAT TCTTTGTTTT 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RNA immunoprecipitation identifies novel targets of DAZL in human foetal ovary.
Molecular human reproduction23(3)177-186(2017 Mar)
Rosario R,Smith RW,Adams IR,Anderson RA

SMC1B is present in mammalian somatic cells and interacts with mitotic cohesin proteins.
Scientific reports518472(2015 Dec)
Mannini L,Cucco F,Quarantotti V,Amato C,Tinti M,Tana L,Frattini A,Delia D,Krantz ID,Jessberger R,Musio A

Protein deep sequencing applied to biobank samples from patients with pancreatic cancer.
Journal of cancer research and clinical oncology141(2)369-80(2015 Feb)
Ansari D,Andersson R,Bauden MP,Andersson B,Connolly JB,Welinder C,Sasor A,Marko-Varga G

RAD21L, a novel cohesin subunit implicated in linking homologous chromosomes in mammalian meiosis.
The Journal of cell biology192(2)263-76(2011 Jan)
Lee J,Hirano T

The evolution of SMC proteins: phylogenetic analysis and structural implications.
Molecular biology and evolution21(2)332-47(2004 Feb)
Cobbe N,Heck MM

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SMC1B cDNA ORF clone, Homo sapiens(human)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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