Ackr1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ackr1
Entrez Gene ID	13349
Full Name	atypical chemokine receptor 1 (Duffy blood group)
Synonyms	AA162249,CCBP1,CD234,Darc,Dfy,ESTM35,FY,GPD
General protein information	Preferred Names atypical chemokine receptor 1 (Duffy blood group) Names atypical chemokine receptor 1 Duffy antigen receptor for chemokines Duffy blood group, chemokine receptor duffy antigen/chemokine receptor glycoprotein D
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 H3\|1 80.33 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_010045.2	NP_034175.2	atypical chemokine receptor 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	DARC	XP_005641007.1
Mus musculus (house mouse)	Darc	NP_034175.2
Rattus norvegicus (Norway rat)	Darc	XP_002728079.3
Bos taurus (cattle)	DARC	NP_001015634.1
Pan troglodytes (chimpanzee)	DARC	XP_001170629.2
Homo sapiens (human)	DARC	NP_002027.2
	DARC	XP_001117200.1

Gene Ontology
Bibliography

Related articles in PubMed

Deletion of the Duffy antigen receptor for chemokines (DARC) promotes insulin resistance and adipose tissue inflammation during high fat feeding.
Benson TW, Weintraub DS, Crowe M, Yiew NKH, Popoola O, Pillai A, Joseph J, Archer K, Greenway C, Chatterjee TK, Mintz J, Stepp DW, Stansfield BK, Chen W, Brittain J, Bogdanov VY, Gao Y, Wilson JG, Tang Y, Kim HW, Weintraub NL
Molecular and cellular endocrinology(2018 Jan)

Atypical chemokine receptor 1 on nucleated erythroid cells regulates hematopoiesis.
Duchene J, Novitzky-Basso I, Thiriot A, Casanova-Acebes M, Bianchini M, Etheridge SL, Hub E, Nitz K, Artinger K, Eller K, Caama?o J, R?licke T, Moss P, Megens RTA, von Andrian UH, Hidalgo A, Weber C, Rot A
Nature immunology18(7)753-761(2017 Jul)

Differential DARC/ACKR1 expression distinguishes venular from non-venular endothelial cells in murine tissues.
Thiriot A, Perdomo C, Cheng G, Novitzky-Basso I, McArdle S, Kishimoto JK, Barreiro O, Mazo I, Triboulet R, Ley K, Rot A, von Andrian UH
BMC biology15(1)45(2017 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nucleated erythroid cells had high expression of ACKR1, which facilitated their direct contact with hematopoietic stem cells. The absence of erythroid ACKR1 altered mouse hematopoiesis including stem and progenitor cells, which ultimately gave rise to phenotypically distinct neutrophils that readily left the circulation, causing neutropenia.
Title: Atypical chemokine receptor 1 on nucleated erythroid cells regulates hematopoiesis.

DARC regulates recruitment of osteoclast precursors at the inflammation site
Title: Potential Role of DARC-Chemokine Interaction in the Recruitment of Osteoclast Precursors in Response to Bacterial Lipopolysaccharide Challenge.

The DARC/CD234 is expressed on macrophages and stabilizes CD82 on long-term repopulating hematopoietic stem cells, promoting their quiescence.
Title: CD82/KAI1 Maintains the Dormancy of Long-Term Hematopoietic Stem Cells through Interaction with DARC-Expressing Macrophages.

Ackr1 deficiency appears to be protective in the ApoE knockout model of atherogenesis, but it is associated with only modest changes in cytokine and chemokine expression as well as T-cell subset frequency and inflammatory macrophage content.
Title: Atypical chemokine receptor 1 deficiency reduces atherogenesis in ApoE-knockout mice.

the results suggest that normal motor function and behavior may partly depend on negative regulation of Purkinje cell activity by Ackr1.
Title: Regulation of motor function and behavior by atypical chemokine receptor 1.

The following Ackr1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ackr1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu23608	NM_010045.2 Latest version!	Mus musculus atypical chemokine receptor 1 (Duffy blood group) (Ackr1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ackr1 ( NM_010045.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034175.2 Mus musculus atypical chemokine receptor 1 (Duffy blood group) (Ackr1), mRNA.

Price & Availability↑

CloneID	OMu23608
Clone ID Related Accession (Same CDS sequence)	NM_010045.2
Accession Version	NM_010045.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	atypical chemokine receptor 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC087781.17. On Mar 14, 2007 this sequence version replaced NM_010045.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF109159.1, BC005583.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGAACT GTCTGTATCC GGTGGAAAAC CTTTCACTAG ACAAGAATGG AACTCAGTTT 
ACTTTCGACA GCTGGAATTA TTCGTTTGAA GATAACTACT CCTATGAACT CTCGAGTGAC 
TACAGCCTGA CACCAGCTGC TCCCTGCTAC TCCTGTAATC TGCTTGGCAG GTCTTCCCTT 
CCCTTCTTCA TGCTCACCAG TGTCCTGGGC ATGCTGGCAA GTGGCGGCAT CCTCTTCGCG 
ATTCTCAGAC CTTTCTTCCA CTGGCAGATT TGCCCCAGCT GGCCCATTCT GGCAGAGTTA 
GCAGTGGGCA GTGCCCTGTT CAGCATTGCA GTGCCCATCC TGGCACCAGG CTTACACAGC 
GCCCACAGCA CAGCCCTATG CAACCTGGGC TACTGGGTAT GGTATACTTC TGCTTTTGCC 
CAAGCTCTGT TGATAGGATG CTATGCTTGC CTGAACCCCA GACTGAATAT TGGTCAACTC 
CGTGGCTTCA CCTTGGGACT CAGTGTGGGA CTTTGGGGAG CAGCTGCCCT CTTAGGGCTG 
CCAGTCGCCC TGGCCAGTGA TGCTTACAAT GGCTTCTGCG CCTTTCCATC CTCCAGAGAC 
ATGGAAGCTT TGAAGTACAT GCATTATGCC ATCTGTTTTA CCATCTTCAC TGTATTGCCA 
CCGACTCTTT TGGCAGCCAA GGGGCTGAAG ATAGCACTGA GCAAGGGGCC TGGCCCCTGG 
GTTAGTGTCT TGTGGATCTG GTTCATTTTC TGGTGGCCTC ATGGGATGGT TCTCATATTT 
GATGCTTTGG TGAGGTCCAA AATTGTTCTT CTGTATACAT GCCAATCCCA GAAGATTCTT 
GATGCGATGC TGAATGTGAC AGAAGCCCTG AGTATGCTGC ACTGTGTGGC TACCCCACTG 
CTCCTGGCCC TGTTCTGCCA TCAGACCACC CGTAGATCCT TGTCCTCACT CTCCCTCCCT 
ACAAGACAGG CTTCTCAAAT GGATGCCCTT GCAGGCAAGT CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034175.2
CDS	94..1098
Translation	MGNCLYPVENLSLDKNGTQFTFDSWNYSFEDNYSYELSSDYSLTPAAPCYSCNLLGRSSLPFFMLTSVLGMLASGGILFAILRPFFHWQICPSWPILAELAVGSALFSIAVPILAPGLHSAHSTALCNLGYWVWYTSAFAQALLIGCYACLNPRLNIGQLRGFTLGLSVGLWGAAALLGLPVALASDAYNGFCAFPSSRDMEALKYMHYAICFTIFTVLPPTLLAAKGLKIALSKGPGPWVSVLWIWFIFWWPHGMVLIFDALVRSKIVLLYTCQSQKILDAMLNVTEALSMLHCVATPLLLALFCHQTTRRSLSSLSLPTRQASQMDALAGKS

Target ORF information:

RefSeq Version	NM_010045.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus atypical chemokine receptor 1 (Duffy blood group) (Ackr1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010045.2

ORF Insert Sequence:

ATGGGGAACT GTCTGTATCC GGTGGAAAAC CTTTCACTAG ACAAGAATGG AACTCAGTTT 
ACTTTCGACA GCTGGAATTA TTCGTTTGAA GATAACTACT CCTATGAACT CTCGAGTGAC 
TACAGCCTGA CACCAGCTGC TCCCTGCTAC TCCTGTAATC TGCTTGGCAG GTCTTCCCTT 
CCCTTCTTCA TGCTCACCAG TGTCCTGGGC ATGCTGGCAA GTGGCGGCAT CCTCTTCGCG 
ATTCTCAGAC CTTTCTTCCA CTGGCAGATT TGCCCCAGCT GGCCCATTCT GGCAGAGTTA 
GCAGTGGGCA GTGCCCTGTT CAGCATTGCA GTGCCCATCC TGGCACCAGG CTTACACAGC 
GCCCACAGCA CAGCCCTATG CAACCTGGGC TACTGGGTAT GGTATACTTC TGCTTTTGCC 
CAAGCTCTGT TGATAGGATG CTATGCTTGC CTGAACCCCA GACTGAATAT TGGTCAACTC 
CGTGGCTTCA CCTTGGGACT CAGTGTGGGA CTTTGGGGAG CAGCTGCCCT CTTAGGGCTG 
CCAGTCGCCC TGGCCAGTGA TGCTTACAAT GGCTTCTGCG CCTTTCCATC CTCCAGAGAC 
ATGGAAGCTT TGAAGTACAT GCATTATGCC ATCTGTTTTA CCATCTTCAC TGTATTGCCA 
CCGACTCTTT TGGCAGCCAA GGGGCTGAAG ATAGCACTGA GCAAGGGGCC TGGCCCCTGG 
GTTAGTGTCT TGTGGATCTG GTTCATTTTC TGGTGGCCTC ATGGGATGGT TCTCATATTT 
GATGCTTTGG TGAGGTCCAA AATTGTTCTT CTGTATACAT GCCAATCCCA GAAGATTCTT 
GATGCGATGC TGAATGTGAC AGAAGCCCTG AGTATGCTGC ACTGTGTGGC TACCCCACTG 
CTCCTGGCCC TGTTCTGCCA TCAGACCACC CGTAGATCCT TGTCCTCACT CTCCCTCCCT 
ACAAGACAGG CTTCTCAAAT GGATGCCCTT GCAGGCAAGT CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Deletion of the Duffy antigen receptor for chemokines (DARC) promotes insulin resistance and adipose tissue inflammation during high fat feeding.
Molecular and cellular endocrinology(2018 Jan)
Benson TW,Weintraub DS,Crowe M,Yiew NKH,Popoola O,Pillai A,Joseph J,Archer K,Greenway C,Chatterjee TK,Mintz J,Stepp DW,Stansfield BK,Chen W,Brittain J,Bogdanov VY,Gao Y,Wilson JG,Tang Y,Kim HW,Weintraub NL

Atypical chemokine receptor 1 on nucleated erythroid cells regulates hematopoiesis.
Nature immunology18(7)753-761(2017 Jul)
Duchene J,Novitzky-Basso I,Thiriot A,Casanova-Acebes M,Bianchini M,Etheridge SL,Hub E,Nitz K,Artinger K,Eller K,Caama?o J,R?licke T,Moss P,Megens RTA,von Andrian UH,Hidalgo A,Weber C,Rot A

Differential DARC/ACKR1 expression distinguishes venular from non-venular endothelial cells in murine tissues.
BMC biology15(1)45(2017 May)
Thiriot A,Perdomo C,Cheng G,Novitzky-Basso I,McArdle S,Kishimoto JK,Barreiro O,Mazo I,Triboulet R,Ley K,Rot A,von Andrian UH

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Ackr1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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