Cst3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cst3
Entrez Gene ID	13010
Full Name	cystatin C
Synonyms	CysC
General protein information	Preferred Names cystatin C Names cystatin-C cystatin-3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 G3\|2 73.6 cM
Summary	The protein encoded by this gene is a cysteine protease inhibitor involved in neurodegenerative and cardiovascular processes. The encoded protein inhibits aggregation of beta-amyloid protein, a hallmark of Alzheimer's disease, so it may be useful as a therapeutic. This protein also may be a biomarker for atherosclerosis. [provided by RefSeq, Aug 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_009976.4	NP_034106.2	cystatin-C precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LOC607874	XP_003639869.1
Pan troglodytes (chimpanzee)	CST3	XP_001146702.2
	CST3	NP_001028096.1
Gallus gallus (chicken)	CST3	NP_990831.2
Homo sapiens (human)	CST3	NP_000090.1
Xenopus tropicalis (tropical clawed frog)	LOC100485774	XP_002934915.1
Bos taurus (cattle)	CST3	NP_776454.1
Mus musculus (house mouse)	Cst3	NP_034106.2
Rattus norvegicus (Norway rat)	Cst3	NP_036969.1

Gene Ontology
Bibliography

Related articles in PubMed

Analogous mechanism regulating formation of neocortical basal radial glia and cerebellar Bergmann glia.
Heng X, Guo Q, Leung AW, Li JY
eLife6(2017 May)

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Naba A, Clauser KR, Mani DR, Carr SA, Hynes RO
Scientific reports740495(2017 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CysC plays an important regulatory role in combating cell death via the autophagic pathway in atherosclerosis.
Title: Autophagy dysfunction and regulatory cystatin C in macrophage death of atherosclerosis.

Data (including data from studies in mutant mice) suggest that up-regulation of Cst3, as observed in plasma of mice with type 2 diabetes, down-regulates insulin signaling and promotes endoplasmic reticulum stress in hepatocytes but not in myotubes.
Title: Cystatin C attenuates insulin signaling transduction by promoting endoplasmic reticulum stress in hepatocytes.

The neuroprotective activity of CysC against Amyotrophic lateral sclerosis-linked mutant Cu/Zn-superoxide dismutase (SOD1)-mediated toxicity.
Title: Cystatin C protects neuronal cells against mutant copper-zinc superoxide dismutase-mediated toxicity.

an important role for macrophages, DC, and ROS in diseases associated with the protease inhibitory activity or amyloidogenic properties of cystatin C.
Title: Developmental regulation of synthesis and dimerization of the amyloidogenic protease inhibitor cystatin C in the hematopoietic system.

Cystatin C is a potential pathogenic signal triggering neurodegeneration in multiple system atrophy.
Title: Cystatin C triggers neuronal degeneration in a model of multiple system atrophy.

The following Cst3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cst3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu05257	NM_009976.4 Latest version!	Mus musculus cystatin C (Cst3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cst3 ( NM_009976.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034106.2 Mus musculus cystatin C (Cst3), mRNA.

Price & Availability↑

CloneID	OMu05257
Clone ID Related Accession (Same CDS sequence)	NM_009976.4
Accession Version	NM_009976.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Mus musculus(house mouse)
Product	cystatin-C precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CK621009.1, AK002438.1 and AI835804.1. On Aug 20, 2015 this sequence version replaced NM_009976.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CA457140.1, CB205647.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAGCC CGCTGCGCTC CTTGCTGTTC CTGCTGGCCG TCCTGGCCGT GGCCTGGGCG 
GCGACCCCAA AACAAGGCCC GCGAATGTTG GGAGCCCCGG AGGAGGCAGA TGCCAATGAG 
GAAGGCGTGC GGCGAGCGTT GGACTTCGCT GTGAGCGAGT ACAACAAGGG CAGCAACGAT 
GCGTACCACA GCCGCGCCAT ACAGGTGGTG AGAGCTCGTA AGCAGCTCGT GGCTGGAGTG 
AACTATTTTT TGGATGTGGA GATGGGCCGA ACTACATGTA CCAAGTCCCA GACAAATTTG 
ACTGACTGTC CTTTCCATGA CCAGCCCCAT CTGATGAGGA AGGCACTCTG CTCCTTCCAG 
ATCTACAGCG TGCCCTGGAA AGGCACACAC TCCCTGACAA AATTCAGCTG CAAAAATGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034106.2
CDS	136..558
Translation	MASPLRSLLFLLAVLAVAWAATPKQGPRMLGAPEEADANEEGVRRALDFAVSEYNKGSNDAYHSRAIQVVRARKQLVAGVNYFLDVEMGRTTCTKSQTNLTDCPFHDQPHLMRKALCSFQIYSVPWKGTHSLTKFSCKNA

Target ORF information:

RefSeq Version	NM_009976.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus cystatin C (Cst3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009976.4

ORF Insert Sequence:

ATGGCCAGCC CGCTGCGCTC CTTGCTGTTC CTGCTGGCCG TCCTGGCCGT GGCCTGGGCG 
GCGACCCCAA AACAAGGCCC GCGAATGTTG GGAGCCCCGG AGGAGGCAGA TGCCAATGAG 
GAAGGCGTGC GGCGAGCGTT GGACTTCGCT GTGAGCGAGT ACAACAAGGG CAGCAACGAT 
GCGTACCACA GCCGCGCCAT ACAGGTGGTG AGAGCTCGTA AGCAGCTCGT GGCTGGAGTG 
AACTATTTTT TGGATGTGGA GATGGGCCGA ACTACATGTA CCAAGTCCCA GACAAATTTG 
ACTGACTGTC CTTTCCATGA CCAGCCCCAT CTGATGAGGA AGGCACTCTG CTCCTTCCAG 
ATCTACAGCG TGCCCTGGAA AGGCACACAC TCCCTGACAA AATTCAGCTG CAAAAATGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Analogous mechanism regulating formation of neocortical basal radial glia and cerebellar Bergmann glia.
eLife6(2017 May)
Heng X,Guo Q,Leung AW,Li JY

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Scientific reports740495(2017 Jan)
Naba A,Clauser KR,Mani DR,Carr SA,Hynes RO

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Cst3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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