Cenpb cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cenpb
Entrez Gene ID	12616
Full Name	centromere protein B
Synonyms	CENP-B
General protein information	Preferred Names centromere protein B Names major centromere autoantigen B centromere autoantigen B
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 F1\|2 63.29 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_007682.3	NP_031708.2	major centromere autoantigen B

Pathways from BioSystems

Homologies

Homo sapiens (human)	CENPB	NP_001801.1
Mus musculus (house mouse)	Cenpb	NP_031708.2
Canis lupus familiaris (dog)	CENPB	XP_851406.2

Gene Ontology
Bibliography

Related articles in PubMed

Alteration/Deficiency in Activation 3 (ADA3) Protein, a Cell Cycle Regulator, Associates with the Centromere through CENP-B and Regulates Chromosome Segregation.
Mohibi S, Srivastava S, Wang-France J, Mirza S, Zhao X, Band H, Band V
The Journal of biological chemistry290(47)28299-310(2015 Nov)

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Thompson CL, Ng L, Menon V, Martinez S, Lee CK, Glattfelder K, Sunkin SM, Henry A, Lau C, Dang C, Garcia-Lopez R, Martinez-Ferre A, Pombero A, Rubenstein JLR, Wakeman WB, Hohmann J, Dee N, Sodt AJ, Young R, Smith K, Nguyen TN, Kidney J, Kuan L, Jeromin A, Kaykas A, Miller J, Page D, Orta G, Bernard A, Riley Z, Smith S, Wohnoutka P, Hawrylycz MJ, Puelles L, Jones AR
Neuron83(2)309-323(2014 Jul)

CENP-B controls centromere formation depending on the chromatin context.
Okada T, Ohzeki J, Nakano M, Yoda K, Brinkley WR, Larionov V, Masumoto H
Cell131(7)1287-300(2007 Dec)

Centromeric protein CENP-B proteasomal degradation induced by the viral protein ICP0.
Lomonte P, Morency E
FEBS letters581(4)658-62(2007 Feb)

Fine mapping of a QTL region with large effects on growth and fatness on mouse chromosome 2.
Jerez-Timaure NC, Eisen EJ, Pomp D
Physiological genomics21(3)411-22(2005 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

demonstrate a novel ADA3 interaction with CENP-B-centromere that may account for its previously known function in mitosis
Title: Alteration/Deficiency in Activation 3 (ADA3) Protein, a Cell Cycle Regulator, Associates with the Centromere through CENP-B and Regulates Chromosome Segregation.

Propose that CENP-B plays a dual role in centromere formation, ensuring de novo formation on DNA lacking a functional centromere but preventing the formation of excess centromeres on chromosomes.
Title: CENP-B controls centromere formation depending on the chromatin context.

This study shows that ICP0 induces the proteasomal-dependent degradation of the centromeric protein CENP-B in infected as well as ICP0-expressing cells.
Title: Centromeric protein CENP-B proteasomal degradation induced by the viral protein ICP0.

Cenpa, Cenpb, and Bub3, but not Cenpc, interacted with PARP-1
Title: Centromere proteins Cenpa, Cenpb, and Bub3 interact with poly(ADP-ribose) polymerase-1 protein and are poly(ADP-ribosyl)ated.

The following Cenpb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cenpb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu83494	NM_007682.3 Latest version!	Mus musculus centromere protein B (Cenpb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cenpb ( NM_007682.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031708.2 Mus musculus centromere protein B (Cenpb), mRNA.

Price & Availability↑

CloneID	OMu83494
Clone ID Related Accession (Same CDS sequence)	NM_007682.3
Accession Version	NM_007682.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	major centromere autoantigen B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC071269.1. On Nov 17, 2015 this sequence version replaced NM_007682.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: BC071269.1 [ECO:0000345] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCCCCA AGCGGCGGCA GCTGACGTTC CGGGAGAAGT CGCGGATCAT CCAGGAGGTG 
GAGGAGAACC CGGACCTACG CAAGGGCGAG ATCGCGCGGC GCTTCAACAT CCCGCCGTCC 
ACGCTGAGCA CCATCCTGAA GAACAAGCGC GCCATCCTGG CGTCGGAGCG CAAGTACGGA 
GTGGCCTCCA CCTGCCGCAA GACCAACAAG CTGTCCCCGT ACGACAAGCT GGAGGGGCTT 
CTCATCGCTT GGTTCCAGCA GATCCGCGCC GCCGGGCTGC CGGTCAAGGG CATCATCCTG 
AAAGAGAAGG CGCTGCGGAT AGCGGAGGAG CTGGGCATGG ACGACTTCAC TGCTTCCAAC 
GGCTGGCTGG ATCGCTTCCG CAGGCGCCAC GGTGTAGTGG CCTGCAGCGG CGTGACCCGT 
TCTCGGGCGC GAAGCTCTGC CCCTCGGGCC CCAGCGGCAC CTGCCGGCCC AGCCACGGTG 
CCCTCCGAGG GCAGCGGTGG GAGCACACCC GGCTGGCACA CGAGGGAGGA GCAGCCGCCG 
TCGGTAGCTG AGGGCTACGC CTCGCAGGAC GTGTTCAGCG CCACCGAGAC CAGCCTGTGG 
TACGACTTTC TGTCGGACCA GGCCTCAGGG CTCTGGGGAG GTGATGGACC GGCGCGCCAG 
GCCACCCAGC GCCTTAGCGT TTTGCTGTGC GCCAACGCCG ATGGCAGCGA GAAGCTTCCC 
CCACTGGTCG CAGGCAAATC TGCCAAGCCC CGTGCAGGGC AAGGTGGTCT GCCCTGCGAC 
TACACTGCCA ACTCGAAGGG TGGAGTCACC ACCCAGGCTC TGGCTAAGTA CTTGAAAGCT 
CTGGACACTC GAATGGCAGC AGAATCTCGT CGGGTCCTTC TGCTGGCCGG CCGTCTGGCT 
GCCCAGTCCT TGGACACCTC GGGCCTGCGG CACGTGCAGC TGGCCTTCTT CCCTCCTGGC 
ACCGTGCATC CTTTGGAGCG AGGAGTGGTC CAACAGGTGA AGGGCCACTA CCGCCAGGCG 
ATGTTGCTCA AGGCCATGGC AGCACTCGAG GGCCAGGATC CCTCAGGCTT GCAACTGGGC 
CTTGTGGAGG CCCTGCACTT TGTGGCTGCA GCCTGGCAGG CAGTGGAGCC CTCGGACATA 
GCAACTTGCT TTCGTGAGGC GGGTTTTGGA GGTGGCCTTA ATGCCACTAT CACCACTTCC 
TTCAAGAGTG AGGGAGAGGA AGAGGAGGAA GAGGAGGAGG AAGAAGAGGA GGAGGAGGAA 
GAGGAGGGTG AAGGGGAAGA GGAGGAGGAA GAGGAAGAAG AAGGGGAGGA GGAAGGAGGG 
GAAGGAGAGG AGGAAGGAGA GGAAGAGGTA GAAGAGGAAG GTGAGGTTGA TGATAGTGAT 
GAAGAGGAGG AAGAAAGTTC CTCTGAGGGT TTAGAGGCTG AAGACTGGGC CCAGGGAGTA 
GTGGAAGCCA GTGGTGGTTT TGGGGGTTAT AGTGTCCAGG AAGAGGCCCA GTTCCCCACT 
CTCCATTTCC TGGAAGGAGG AGAGGACTCT GACTCAGACA GTGATGAAGA GGAGGACGAT 
GAAGAGGAGG ATGAGGAGGA TGAAGATGAG GAGGATGATG AGGATGGTGA TGAGGTCCCT 
GTGCCCAGCT TTGGGGAGGC CATGGCTTAC TTTGCCATGG TCAAGAGGTA CCTGACCTCC 
TTCCCCATCG ATGACCGAGT GCAGAGTCAC ATCCTTCATT TGGAACACGA TCTGGTCCAC 
GTGACTAGGA AGAACCATGC CAGGCAGGCG GGAGTTCGGG GTCTTGGACA TCAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031708.2
CDS	192..1991
Translation	MGPKRRQLTFREKSRIIQEVEENPDLRKGEIARRFNIPPSTLSTILKNKRAILASERKYGVASTCRKTNKLSPYDKLEGLLIAWFQQIRAAGLPVKGIILKEKALRIAEELGMDDFTASNGWLDRFRRRHGVVACSGVTRSRARSSAPRAPAAPAGPATVPSEGSGGSTPGWHTREEQPPSVAEGYASQDVFSATETSLWYDFLSDQASGLWGGDGPARQATQRLSVLLCANADGSEKLPPLVAGKSAKPRAGQGGLPCDYTANSKGGVTTQALAKYLKALDTRMAAESRRVLLLAGRLAAQSLDTSGLRHVQLAFFPPGTVHPLERGVVQQVKGHYRQAMLLKAMAALEGQDPSGLQLGLVEALHFVAAAWQAVEPSDIATCFREAGFGGGLNATITTSFKSEGEEEEEEEEEEEEEEEEEGEGEEEEEEEEEGEEEGGEGEEEGEEEVEEEGEVDDSDEEEEESSSEGLEAEDWAQGVVEASGGFGGYSVQEEAQFPTLHFLEGGEDSDSDSDEEEDDEEEDEEDEDEEDDEDGDEVPVPSFGEAMAYFAMVKRYLTSFPIDDRVQSHILHLEHDLVHVTRKNHARQAGVRGLGHQS

Target ORF information:

RefSeq Version	NM_007682.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus centromere protein B (Cenpb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007682.3

ORF Insert Sequence:

ATGGGCCCCA AGCGGCGGCA GCTGACGTTC CGGGAGAAGT CGCGGATCAT CCAGGAGGTG 
GAGGAGAACC CGGACCTACG CAAGGGCGAG ATCGCGCGGC GCTTCAACAT CCCGCCGTCC 
ACGCTGAGCA CCATCCTGAA GAACAAGCGC GCCATCCTGG CGTCGGAGCG CAAGTACGGA 
GTGGCCTCCA CCTGCCGCAA GACCAACAAG CTGTCCCCGT ACGACAAGCT GGAGGGGCTT 
CTCATCGCTT GGTTCCAGCA GATCCGCGCC GCCGGGCTGC CGGTCAAGGG CATCATCCTG 
AAAGAGAAGG CGCTGCGGAT AGCGGAGGAG CTGGGCATGG ACGACTTCAC TGCTTCCAAC 
GGCTGGCTGG ATCGCTTCCG CAGGCGCCAC GGTGTAGTGG CCTGCAGCGG CGTGACCCGT 
TCTCGGGCGC GAAGCTCTGC CCCTCGGGCC CCAGCGGCAC CTGCCGGCCC AGCCACGGTG 
CCCTCCGAGG GCAGCGGTGG GAGCACACCC GGCTGGCACA CGAGGGAGGA GCAGCCGCCG 
TCGGTAGCTG AGGGCTACGC CTCGCAGGAC GTGTTCAGCG CCACCGAGAC CAGCCTGTGG 
TACGACTTTC TGTCGGACCA GGCCTCAGGG CTCTGGGGAG GTGATGGACC GGCGCGCCAG 
GCCACCCAGC GCCTTAGCGT TTTGCTGTGC GCCAACGCCG ATGGCAGCGA GAAGCTTCCC 
CCACTGGTCG CAGGCAAATC TGCCAAGCCC CGTGCAGGGC AAGGTGGTCT GCCCTGCGAC 
TACACTGCCA ACTCGAAGGG TGGAGTCACC ACCCAGGCTC TGGCTAAGTA CTTGAAAGCT 
CTGGACACTC GAATGGCAGC AGAATCTCGT CGGGTCCTTC TGCTGGCCGG CCGTCTGGCT 
GCCCAGTCCT TGGACACCTC GGGCCTGCGG CACGTGCAGC TGGCCTTCTT CCCTCCTGGC 
ACCGTGCATC CTTTGGAGCG AGGAGTGGTC CAACAGGTGA AGGGCCACTA CCGCCAGGCG 
ATGTTGCTCA AGGCCATGGC AGCACTCGAG GGCCAGGATC CCTCAGGCTT GCAACTGGGC 
CTTGTGGAGG CCCTGCACTT TGTGGCTGCA GCCTGGCAGG CAGTGGAGCC CTCGGACATA 
GCAACTTGCT TTCGTGAGGC GGGTTTTGGA GGTGGCCTTA ATGCCACTAT CACCACTTCC 
TTCAAGAGTG AGGGAGAGGA AGAGGAGGAA GAGGAGGAGG AAGAAGAGGA GGAGGAGGAA 
GAGGAGGGTG AAGGGGAAGA GGAGGAGGAA GAGGAAGAAG AAGGGGAGGA GGAAGGAGGG 
GAAGGAGAGG AGGAAGGAGA GGAAGAGGTA GAAGAGGAAG GTGAGGTTGA TGATAGTGAT 
GAAGAGGAGG AAGAAAGTTC CTCTGAGGGT TTAGAGGCTG AAGACTGGGC CCAGGGAGTA 
GTGGAAGCCA GTGGTGGTTT TGGGGGTTAT AGTGTCCAGG AAGAGGCCCA GTTCCCCACT 
CTCCATTTCC TGGAAGGAGG AGAGGACTCT GACTCAGACA GTGATGAAGA GGAGGACGAT 
GAAGAGGAGG ATGAGGAGGA TGAAGATGAG GAGGATGATG AGGATGGTGA TGAGGTCCCT 
GTGCCCAGCT TTGGGGAGGC CATGGCTTAC TTTGCCATGG TCAAGAGGTA CCTGACCTCC 
TTCCCCATCG ATGACCGAGT GCAGAGTCAC ATCCTTCATT TGGAACACGA TCTGGTCCAC 
GTGACTAGGA AGAACCATGC CAGGCAGGCG GGAGTTCGGG GTCTTGGACA TCAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Alteration/Deficiency in Activation 3 (ADA3) Protein, a Cell Cycle Regulator, Associates with the Centromere through CENP-B and Regulates Chromosome Segregation.
The Journal of biological chemistry290(47)28299-310(2015 Nov)
Mohibi S,Srivastava S,Wang-France J,Mirza S,Zhao X,Band H,Band V

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Neuron83(2)309-323(2014 Jul)
Thompson CL,Ng L,Menon V,Martinez S,Lee CK,Glattfelder K,Sunkin SM,Henry A,Lau C,Dang C,Garcia-Lopez R,Martinez-Ferre A,Pombero A,Rubenstein JLR,Wakeman WB,Hohmann J,Dee N,Sodt AJ,Young R,Smith K,Nguyen TN,Kidney J,Kuan L,Jeromin A,Kaykas A,Miller J,Page D,Orta G,Bernard A,Riley Z,Smith S,Wohnoutka P,Hawrylycz MJ,Puelles L,Jones AR

CENP-B controls centromere formation depending on the chromatin context.
Cell131(7)1287-300(2007 Dec)
Okada T,Ohzeki J,Nakano M,Yoda K,Brinkley WR,Larionov V,Masumoto H

Centromeric protein CENP-B proteasomal degradation induced by the viral protein ICP0.
FEBS letters581(4)658-62(2007 Feb)
Lomonte P,Morency E

Fine mapping of a QTL region with large effects on growth and fatness on mouse chromosome 2.
Physiological genomics21(3)411-22(2005 May)
Jerez-Timaure NC,Eisen EJ,Pomp D