CENPB cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CENPB
Entrez Gene ID	1059
Full Name	centromere protein B
General protein information	Preferred Names centromere protein B Names major centromere autoantigen B CENP-B centromere autoantigen B centromere protein B, 80kDa
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 20 Map Location: 20p13
Summary	This gene product is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies. [provided by RefSeq, Jul 2008].
Disorder MIM:	117140

mRNA and Protein(s)

mRNA	Protein	Name
NM_001810.6	NP_001801.1	major centromere autoantigen B
NM_001810.5	NP_001801.1	major centromere autoantigen B

Pathways from BioSystems

Homologies

Homo sapiens (human)	CENPB	NP_001801.1
Mus musculus (house mouse)	Cenpb	NP_031708.2
Canis lupus familiaris (dog)	CENPB	XP_851406.2

Gene Ontology
Bibliography

Related articles in PubMed

CENP-B protects centromere chromatin integrity by facilitating histone deposition via the H3.3-specific chaperone Daxx.
Morozov VM, Giovinazzi S, Ishov AM
Epigenetics & chromatin10(1)63(2017 Dec)

Mutational spectrum of CENP-B box and ?-satellite DNA on chromosome 21 in Down syndrome children.
Chen Q, Tan B, He JL, Liu XQ, Chen XM, Gao RF, Zhu J, Wang YX, Qi HB
Molecular medicine reports15(4)2313-2317(2017 Apr)

Enrichment of Scleroderma Vascular Disease-Associated Autoantigens in Endothelial Lineage Cells.
McMahan ZH, Cottrell TR, Wigley FM, Antiochos B, Zambidis ET, Park TS, Halushka MK, Gutierrez-Alamillo L, Cimbro R, Rosen A, Casciola-Rosen L
Arthritis & rheumatology (Hoboken, N.J.)68(10)2540-9(2016 10)

CENPT bridges adjacent CENPA nucleosomes on young human ?-satellite dimers.
Thakur J, Henikoff S
Genome research26(9)1178-87(2016 Sep)

DNA Sequence-Specific Binding of CENP-B Enhances the Fidelity of Human Centromere Function.
Fachinetti D, Han JS, McMahon MA, Ly P, Abdullah A, Wong AJ, Cleveland DW
Developmental cell33(3)314-27(2015 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results indicated that CENPB boxes are highly conserved in Down syndrome (DS) patients and may not be responsible for Chr21 nondisjunction events. However, alphasatDNA in Chr21 is variable and deoxynucleotide deletions, mutations and polymorphisms may act as potential molecular diagnostic markers of DS.
Title: Mutational spectrum of CENP-B box and ?-satellite DNA on chromosome 21 in Down syndrome children.

Findings indicate that expression of the scleroderma autoantigens IFI-16 and CENPs, which are associated with severe vascular disease, is increased in vascular progenitors and mature endothelial cells. High level, lineage-enriched expression of autoantigens may explain the striking association between clinical phenotypes and the immune targeting of specific autoantigens.
Title: Enrichment of Scleroderma Vascular Disease-Associated Autoantigens in Endothelial Lineage Cells.

Upon cross-linking, the entire CENPA/CENPB/CENPC/CENPT complex is nuclease-protected over an alpha-satellite dimer that comprises the fundamental unit of centromeric chromatin. We conclude that CENPA/CENPC and CENPT pathways for kinetochore assembly are physically integrated over young alpha-satellite dimers.
Title: CENPT bridges adjacent CENPA nucleosomes on young human ?-satellite dimers.

The results revealed that CENP-B binding in the vicinity of the CENP-A nucleosome substantially stabilizes the CENP-A nucleosome on alphoid DNA in human cells.
Title: Stable complex formation of CENP-B with the CENP-A nucleosome.

CENP-B directly binds both CENP-A's amino-terminal tail and CENP-C, a key nucleator of kinetochore assembly
Title: DNA Sequence-Specific Binding of CENP-B Enhances the Fidelity of Human Centromere Function.

The following CENPB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CENPB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu15809	NM_001810.6 Latest version!	Homo sapiens centromere protein B (CENPB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu15809	NM_001810.5	Homo sapiens centromere protein B (CENPB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CENPB ( NM_001810.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_001801.1 Homo sapiens centromere protein B (CENPB), mRNA.

Price & Availability↑

CloneID	OHu15809
Clone ID Related Accession (Same CDS sequence)	NM_001810.6 , NM_001810.5
Accession Version	NM_001810.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	major centromere autoantigen B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Hugh Robertson. The reference sequence was derived from AL109804.41, BC053847.1 and BC003649.2. On Nov 23, 2018 this sequence version replaced NM_001810.5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: BC053847.1, X05299.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000379751.5/ ENSP00000369075.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGCCCCA AGAGGCGACA GCTGACGTTC CGGGAGAAGT CACGGATCAT CCAGGAGGTG 
GAGGAGAATC CGGACCTGCG CAAGGGCGAG ATCGCGCGGC GCTTCAACAT CCCGCCGTCC 
ACGCTGAGCA CGATCCTGAA GAACAAGCGC GCCATCCTGG CGTCGGAGCG CAAGTACGGG 
GTGGCCTCCA CCTGCCGCAA GACCAACAAG CTGTCTCCCT ACGACAAGCT CGAGGGCTTG 
CTCATCGCCT GGTTCCAGCA GATCCGCGCC GCCGGCCTGC CGGTCAAGGG CATCATCCTC 
AAGGAGAAGG CGCTGCGCAT AGCCGAGGAG CTGGGCATGG ACGACTTCAC CGCCTCCAAC 
GGCTGGCTGG ACCGCTTCCG CCGGCGCCAC GGCGTGGTGT CCTGCAGCGG CGTGGCCCGC 
GCCCGCGCGC GAAACGCTGC CCCCCGCACC CCGGCGGCGC CTGCCAGTCC GGCCGCGGTG 
CCCTCGGAGG GCAGTGGCGG GAGCACTACT GGTTGGCGCG CTCGGGAGGA GCAGCCGCCG 
TCGGTGGCCG AGGGCTACGC CTCGCAGGAC GTGTTCAGCG CCACCGAGAC CAGTCTATGG 
TACGACTTCC TGCCCGACCA GGCCGCGGGG CTGTGCGGAG GCGACGGACG GCCGCGTCAA 
GCCACCCAGC GCCTGAGCGT CCTGCTATGC GCCAATGCCG ACGGCAGCGA GAAGCTGCCC 
CCGCTGGTGG CCGGCAAGTC GGCCAAGCCC CGCGCAGGCC AAGCCGGCCT GCCCTGCGAC 
TACACCGCCA ACTCCAAGGG TGGTGTCACC ACCCAGGCCC TGGCCAAGTA CTTGAAGGCC 
TTGGACACCC GAATGGCTGC AGAGTCTCGC CGGGTCCTGC TGTTGGCCGG CCGCTTGGCT 
GCCCAGTCCT TGGACACCTC GGGCCTGCGG CATGTGCAGC TGGCCTTCTT CCCTCCCGGC 
ACCGTGCATC CGCTGGAGAG GGGAGTGGTC CAGCAGGTGA AGGGCCACTA CCGCCAGGCC 
ATGCTGCTCA AGGCCATGGC CGCGCTAGAG GGCCAGGATC CCTCAGGCCT GCAGCTGGGT 
CTCACGGAGG CCCTGCACTT TGTGGCTGCC GCCTGGCAGG CAGTGGAGCC TTCGGACATA 
GCCGCCTGCT TTCGTGAGGC TGGCTTTGGG GGTGGCCCTA ATGCCACCAT CACCACTTCC 
CTCAAGAGTG AGGGAGAGGA AGAGGAGGAG GAGGAGGAAG AAGAGGAGGA GGAAGAGGGT 
GAAGGAGAGG AAGAGGAGGA GGAAGGGGAG GAGGAGGAGG AGGAAGGGGG GGAAGGAGAG 
GAATTGGGGG AGGAAGAGGA GGTGGAGGAG GAGGGTGATG TTGATAGTGA TGAAGAAGAG 
GAGGAAGATG AGGAGAGCTC CTCGGAGGGC TTGGAGGCTG AGGACTGGGC CCAGGGAGTA 
GTGGAGGCCG GTGGCAGCTT CGGGGCTTAT GGTGCCCAGG AGGAAGCCCA GTGCCCTACT 
CTGCATTTCC TGGAAGGTGG GGAGGACTCT GATTCAGACA GTGAGGAAGA GGACGATGAG 
GAAGAGGATG ATGAAGATGA AGACGACGAT GATGATGAGG AGGATGGTGA TGAGGTGCCT 
GTACCCAGCT TTGGGGAGGC CATGGCTTAC TTTGCCATGG TCAAGAGGTA CCTGACCTCC 
TTCCCCATTG ATGACCGCGT GCAGAGCCAC ATCCTCCACT TGGAACACGA TCTGGTTCAT 
GTGACCAGGA AGAACCACGC CAGGCAGGCG GGAGTTCGAG GTCTTGGACA TCAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001801.1
CDS	258..2057
Translation	MGPKRRQLTFREKSRIIQEVEENPDLRKGEIARRFNIPPSTLSTILKNKRAILASERKYGVASTCRKTNKLSPYDKLEGLLIAWFQQIRAAGLPVKGIILKEKALRIAEELGMDDFTASNGWLDRFRRRHGVVSCSGVARARARNAAPRTPAAPASPAAVPSEGSGGSTTGWRAREEQPPSVAEGYASQDVFSATETSLWYDFLPDQAAGLCGGDGRPRQATQRLSVLLCANADGSEKLPPLVAGKSAKPRAGQAGLPCDYTANSKGGVTTQALAKYLKALDTRMAAESRRVLLLAGRLAAQSLDTSGLRHVQLAFFPPGTVHPLERGVVQQVKGHYRQAMLLKAMAALEGQDPSGLQLGLTEALHFVAAAWQAVEPSDIAACFREAGFGGGPNATITTSLKSEGEEEEEEEEEEEEEEGEGEEEEEEGEEEEEEGGEGEELGEEEEVEEEGDVDSDEEEEEDEESSSEGLEAEDWAQGVVEAGGSFGAYGAQEEAQCPTLHFLEGGEDSDSDSEEEDDEEEDDEDEDDDDDEEDGDEVPVPSFGEAMAYFAMVKRYLTSFPIDDRVQSHILHLEHDLVHVTRKNHARQAGVRGLGHQS

Target ORF information:

RefSeq Version	NM_001810.6
Organism	Homo sapiens(human)
Definition	Homo sapiens centromere protein B (CENPB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001810.6

ORF Insert Sequence:

ATGGGCCCCA AGAGGCGACA GCTGACGTTC CGGGAGAAGT CACGGATCAT CCAGGAGGTG 
GAGGAGAATC CGGACCTGCG CAAGGGCGAG ATCGCGCGGC GCTTCAACAT CCCGCCGTCC 
ACGCTGAGCA CGATCCTGAA GAACAAGCGC GCCATCCTGG CGTCGGAGCG CAAGTACGGG 
GTGGCCTCCA CCTGCCGCAA GACCAACAAG CTGTCTCCCT ACGACAAGCT CGAGGGCTTG 
CTCATCGCCT GGTTCCAGCA GATCCGCGCC GCCGGCCTGC CGGTCAAGGG CATCATCCTC 
AAGGAGAAGG CGCTGCGCAT AGCCGAGGAG CTGGGCATGG ACGACTTCAC CGCCTCCAAC 
GGCTGGCTGG ACCGCTTCCG CCGGCGCCAC GGCGTGGTGT CCTGCAGCGG CGTGGCCCGC 
GCCCGCGCGC GAAACGCTGC CCCCCGCACC CCGGCGGCGC CTGCCAGTCC GGCCGCGGTG 
CCCTCGGAGG GCAGTGGCGG GAGCACTACT GGTTGGCGCG CTCGGGAGGA GCAGCCGCCG 
TCGGTGGCCG AGGGCTACGC CTCGCAGGAC GTGTTCAGCG CCACCGAGAC CAGTCTATGG 
TACGACTTCC TGCCCGACCA GGCCGCGGGG CTGTGCGGAG GCGACGGACG GCCGCGTCAA 
GCCACCCAGC GCCTGAGCGT CCTGCTATGC GCCAATGCCG ACGGCAGCGA GAAGCTGCCC 
CCGCTGGTGG CCGGCAAGTC GGCCAAGCCC CGCGCAGGCC AAGCCGGCCT GCCCTGCGAC 
TACACCGCCA ACTCCAAGGG TGGTGTCACC ACCCAGGCCC TGGCCAAGTA CTTGAAGGCC 
TTGGACACCC GAATGGCTGC AGAGTCTCGC CGGGTCCTGC TGTTGGCCGG CCGCTTGGCT 
GCCCAGTCCT TGGACACCTC GGGCCTGCGG CATGTGCAGC TGGCCTTCTT CCCTCCCGGC 
ACCGTGCATC CGCTGGAGAG GGGAGTGGTC CAGCAGGTGA AGGGCCACTA CCGCCAGGCC 
ATGCTGCTCA AGGCCATGGC CGCGCTAGAG GGCCAGGATC CCTCAGGCCT GCAGCTGGGT 
CTCACGGAGG CCCTGCACTT TGTGGCTGCC GCCTGGCAGG CAGTGGAGCC TTCGGACATA 
GCCGCCTGCT TTCGTGAGGC TGGCTTTGGG GGTGGCCCTA ATGCCACCAT CACCACTTCC 
CTCAAGAGTG AGGGAGAGGA AGAGGAGGAG GAGGAGGAAG AAGAGGAGGA GGAAGAGGGT 
GAAGGAGAGG AAGAGGAGGA GGAAGGGGAG GAGGAGGAGG AGGAAGGGGG GGAAGGAGAG 
GAATTGGGGG AGGAAGAGGA GGTGGAGGAG GAGGGTGATG TTGATAGTGA TGAAGAAGAG 
GAGGAAGATG AGGAGAGCTC CTCGGAGGGC TTGGAGGCTG AGGACTGGGC CCAGGGAGTA 
GTGGAGGCCG GTGGCAGCTT CGGGGCTTAT GGTGCCCAGG AGGAAGCCCA GTGCCCTACT 
CTGCATTTCC TGGAAGGTGG GGAGGACTCT GATTCAGACA GTGAGGAAGA GGACGATGAG 
GAAGAGGATG ATGAAGATGA AGACGACGAT GATGATGAGG AGGATGGTGA TGAGGTGCCT 
GTACCCAGCT TTGGGGAGGC CATGGCTTAC TTTGCCATGG TCAAGAGGTA CCTGACCTCC 
TTCCCCATTG ATGACCGCGT GCAGAGCCAC ATCCTCCACT TGGAACACGA TCTGGTTCAT 
GTGACCAGGA AGAACCACGC CAGGCAGGCG GGAGTTCGAG GTCTTGGACA TCAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CENPB ( NM_001810.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001801.1 Homo sapiens centromere protein B (CENPB), mRNA.

Price & Availability↑

CloneID	OHu15809
Clone ID Related Accession (Same CDS sequence)	NM_001810.6 , NM_001810.5
Accession Version	NM_001810.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-28
Organism	Homo sapiens(human)
Product	major centromere autoantigen B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Hugh Robertson. The reference sequence was derived from BC053847.1 and BC003649.2. On Sep 8, 2004 this sequence version replaced NM_001810.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: BC053847.1 [ECO:0000345] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCCCCA AGAGGCGACA GCTGACGTTC CGGGAGAAGT CACGGATCAT CCAGGAGGTG 
GAGGAGAATC CGGACCTGCG CAAGGGCGAG ATCGCGCGGC GCTTCAACAT CCCGCCGTCC 
ACGCTGAGCA CGATCCTGAA GAACAAGCGC GCCATCCTGG CGTCGGAGCG CAAGTACGGG 
GTGGCCTCCA CCTGCCGCAA GACCAACAAG CTGTCTCCCT ACGACAAGCT CGAGGGCTTG 
CTCATCGCCT GGTTCCAGCA GATCCGCGCC GCCGGCCTGC CGGTCAAGGG CATCATCCTC 
AAGGAGAAGG CGCTGCGCAT AGCCGAGGAG CTGGGCATGG ACGACTTCAC CGCCTCCAAC 
GGCTGGCTGG ACCGCTTCCG CCGGCGCCAC GGCGTGGTGT CCTGCAGCGG CGTGGCCCGC 
GCCCGCGCGC GAAACGCTGC CCCCCGCACC CCGGCGGCGC CTGCCAGTCC GGCCGCGGTG 
CCCTCGGAGG GCAGTGGCGG GAGCACTACT GGTTGGCGCG CTCGGGAGGA GCAGCCGCCG 
TCGGTGGCCG AGGGCTACGC CTCGCAGGAC GTGTTCAGCG CCACCGAGAC CAGTCTATGG 
TACGACTTCC TGCCCGACCA GGCCGCGGGG CTGTGCGGAG GCGACGGACG GCCGCGTCAA 
GCCACCCAGC GCCTGAGCGT CCTGCTATGC GCCAATGCCG ACGGCAGCGA GAAGCTGCCC 
CCGCTGGTGG CCGGCAAGTC GGCCAAGCCC CGCGCAGGCC AAGCCGGCCT GCCCTGCGAC 
TACACCGCCA ACTCCAAGGG TGGTGTCACC ACCCAGGCCC TGGCCAAGTA CTTGAAGGCC 
TTGGACACCC GAATGGCTGC AGAGTCTCGC CGGGTCCTGC TGTTGGCCGG CCGCTTGGCT 
GCCCAGTCCT TGGACACCTC GGGCCTGCGG CATGTGCAGC TGGCCTTCTT CCCTCCCGGC 
ACCGTGCATC CGCTGGAGAG GGGAGTGGTC CAGCAGGTGA AGGGCCACTA CCGCCAGGCC 
ATGCTGCTCA AGGCCATGGC CGCGCTAGAG GGCCAGGATC CCTCAGGCCT GCAGCTGGGT 
CTCACGGAGG CCCTGCACTT TGTGGCTGCC GCCTGGCAGG CAGTGGAGCC TTCGGACATA 
GCCGCCTGCT TTCGTGAGGC TGGCTTTGGG GGTGGCCCTA ATGCCACCAT CACCACTTCC 
CTCAAGAGTG AGGGAGAGGA AGAGGAGGAG GAGGAGGAAG AAGAGGAGGA GGAAGAGGGT 
GAAGGAGAGG AAGAGGAGGA GGAAGGGGAG GAGGAGGAGG AGGAAGGGGG GGAAGGAGAG 
GAATTGGGGG AGGAAGAGGA GGTGGAGGAG GAGGGTGATG TTGATAGTGA TGAAGAAGAG 
GAGGAAGATG AGGAGAGCTC CTCGGAGGGC TTGGAGGCTG AGGACTGGGC CCAGGGAGTA 
GTGGAGGCCG GTGGCAGCTT CGGGGCTTAT GGTGCCCAGG AGGAAGCCCA GTGCCCTACT 
CTGCATTTCC TGGAAGGTGG GGAGGACTCT GATTCAGACA GTGAGGAAGA GGACGATGAG 
GAAGAGGATG ATGAAGATGA AGACGACGAT GATGATGAGG AGGATGGTGA TGAGGTGCCT 
GTACCCAGCT TTGGGGAGGC CATGGCTTAC TTTGCCATGG TCAAGAGGTA CCTGACCTCC 
TTCCCCATTG ATGACCGCGT GCAGAGCCAC ATCCTCCACT TGGAACACGA TCTGGTTCAT 
GTGACCAGGA AGAACCACGC CAGGCAGGCG GGAGTTCGAG GTCTTGGACA TCAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001801.1
CDS	208..2007
Translation	MGPKRRQLTFREKSRIIQEVEENPDLRKGEIARRFNIPPSTLSTILKNKRAILASERKYGVASTCRKTNKLSPYDKLEGLLIAWFQQIRAAGLPVKGIILKEKALRIAEELGMDDFTASNGWLDRFRRRHGVVSCSGVARARARNAAPRTPAAPASPAAVPSEGSGGSTTGWRAREEQPPSVAEGYASQDVFSATETSLWYDFLPDQAAGLCGGDGRPRQATQRLSVLLCANADGSEKLPPLVAGKSAKPRAGQAGLPCDYTANSKGGVTTQALAKYLKALDTRMAAESRRVLLLAGRLAAQSLDTSGLRHVQLAFFPPGTVHPLERGVVQQVKGHYRQAMLLKAMAALEGQDPSGLQLGLTEALHFVAAAWQAVEPSDIAACFREAGFGGGPNATITTSLKSEGEEEEEEEEEEEEEEGEGEEEEEEGEEEEEEGGEGEELGEEEEVEEEGDVDSDEEEEEDEESSSEGLEAEDWAQGVVEAGGSFGAYGAQEEAQCPTLHFLEGGEDSDSDSEEEDDEEEDDEDEDDDDDEEDGDEVPVPSFGEAMAYFAMVKRYLTSFPIDDRVQSHILHLEHDLVHVTRKNHARQAGVRGLGHQS

Target ORF information:

RefSeq Version	NM_001810.5
Organism	Homo sapiens(human)
Definition	Homo sapiens centromere protein B (CENPB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001810.5

ORF Insert Sequence:

ATGGGCCCCA AGAGGCGACA GCTGACGTTC CGGGAGAAGT CACGGATCAT CCAGGAGGTG 
GAGGAGAATC CGGACCTGCG CAAGGGCGAG ATCGCGCGGC GCTTCAACAT CCCGCCGTCC 
ACGCTGAGCA CGATCCTGAA GAACAAGCGC GCCATCCTGG CGTCGGAGCG CAAGTACGGG 
GTGGCCTCCA CCTGCCGCAA GACCAACAAG CTGTCTCCCT ACGACAAGCT CGAGGGCTTG 
CTCATCGCCT GGTTCCAGCA GATCCGCGCC GCCGGCCTGC CGGTCAAGGG CATCATCCTC 
AAGGAGAAGG CGCTGCGCAT AGCCGAGGAG CTGGGCATGG ACGACTTCAC CGCCTCCAAC 
GGCTGGCTGG ACCGCTTCCG CCGGCGCCAC GGCGTGGTGT CCTGCAGCGG CGTGGCCCGC 
GCCCGCGCGC GAAACGCTGC CCCCCGCACC CCGGCGGCGC CTGCCAGTCC GGCCGCGGTG 
CCCTCGGAGG GCAGTGGCGG GAGCACTACT GGTTGGCGCG CTCGGGAGGA GCAGCCGCCG 
TCGGTGGCCG AGGGCTACGC CTCGCAGGAC GTGTTCAGCG CCACCGAGAC CAGTCTATGG 
TACGACTTCC TGCCCGACCA GGCCGCGGGG CTGTGCGGAG GCGACGGACG GCCGCGTCAA 
GCCACCCAGC GCCTGAGCGT CCTGCTATGC GCCAATGCCG ACGGCAGCGA GAAGCTGCCC 
CCGCTGGTGG CCGGCAAGTC GGCCAAGCCC CGCGCAGGCC AAGCCGGCCT GCCCTGCGAC 
TACACCGCCA ACTCCAAGGG TGGTGTCACC ACCCAGGCCC TGGCCAAGTA CTTGAAGGCC 
TTGGACACCC GAATGGCTGC AGAGTCTCGC CGGGTCCTGC TGTTGGCCGG CCGCTTGGCT 
GCCCAGTCCT TGGACACCTC GGGCCTGCGG CATGTGCAGC TGGCCTTCTT CCCTCCCGGC 
ACCGTGCATC CGCTGGAGAG GGGAGTGGTC CAGCAGGTGA AGGGCCACTA CCGCCAGGCC 
ATGCTGCTCA AGGCCATGGC CGCGCTAGAG GGCCAGGATC CCTCAGGCCT GCAGCTGGGT 
CTCACGGAGG CCCTGCACTT TGTGGCTGCC GCCTGGCAGG CAGTGGAGCC TTCGGACATA 
GCCGCCTGCT TTCGTGAGGC TGGCTTTGGG GGTGGCCCTA ATGCCACCAT CACCACTTCC 
CTCAAGAGTG AGGGAGAGGA AGAGGAGGAG GAGGAGGAAG AAGAGGAGGA GGAAGAGGGT 
GAAGGAGAGG AAGAGGAGGA GGAAGGGGAG GAGGAGGAGG AGGAAGGGGG GGAAGGAGAG 
GAATTGGGGG AGGAAGAGGA GGTGGAGGAG GAGGGTGATG TTGATAGTGA TGAAGAAGAG 
GAGGAAGATG AGGAGAGCTC CTCGGAGGGC TTGGAGGCTG AGGACTGGGC CCAGGGAGTA 
GTGGAGGCCG GTGGCAGCTT CGGGGCTTAT GGTGCCCAGG AGGAAGCCCA GTGCCCTACT 
CTGCATTTCC TGGAAGGTGG GGAGGACTCT GATTCAGACA GTGAGGAAGA GGACGATGAG 
GAAGAGGATG ATGAAGATGA AGACGACGAT GATGATGAGG AGGATGGTGA TGAGGTGCCT 
GTACCCAGCT TTGGGGAGGC CATGGCTTAC TTTGCCATGG TCAAGAGGTA CCTGACCTCC 
TTCCCCATTG ATGACCGCGT GCAGAGCCAC ATCCTCCACT TGGAACACGA TCTGGTTCAT 
GTGACCAGGA AGAACCACGC CAGGCAGGCG GGAGTTCGAG GTCTTGGACA TCAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CENP-B protects centromere chromatin integrity by facilitating histone deposition via the H3.3-specific chaperone Daxx.
Epigenetics & chromatin10(1)63(2017 Dec)
Morozov VM,Giovinazzi S,Ishov AM

Mutational spectrum of CENP-B box and ?-satellite DNA on chromosome 21 in Down syndrome children.
Molecular medicine reports15(4)2313-2317(2017 Apr)
Chen Q,Tan B,He JL,Liu XQ,Chen XM,Gao RF,Zhu J,Wang YX,Qi HB

Enrichment of Scleroderma Vascular Disease-Associated Autoantigens in Endothelial Lineage Cells.
Arthritis & rheumatology (Hoboken, N.J.)68(10)2540-9(2016 10)
McMahan ZH,Cottrell TR,Wigley FM,Antiochos B,Zambidis ET,Park TS,Halushka MK,Gutierrez-Alamillo L,Cimbro R,Rosen A,Casciola-Rosen L

CENPT bridges adjacent CENPA nucleosomes on young human ?-satellite dimers.
Genome research26(9)1178-87(2016 Sep)
Thakur J,Henikoff S

DNA Sequence-Specific Binding of CENP-B Enhances the Fidelity of Human Centromere Function.
Developmental cell33(3)314-27(2015 May)
Fachinetti D,Han JS,McMahon MA,Ly P,Abdullah A,Wong AJ,Cleveland DW

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CENPB cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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