Krt82 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Krt82
Entrez Gene ID	114566
Full Name	keratin 82
Synonyms	Krt2-20
General protein information	Preferred Names keratin 82 Names keratin, type II cuticular Hb2 K82 keratin complex 2, basic, gene 20 type II hair keratin Hb2 type-II keratin Kb22
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15 F2\|15 56.9 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_053249.3	NP_444479.2	keratin, type II cuticular Hb2

Pathways from BioSystems

Homologies

Homo sapiens (human)	KRT82	NP_149022.3
Canis lupus familiaris (dog)	KRT82	XP_543651.2
Bos taurus (cattle)	KRT82	NP_001076085.1
Pan troglodytes (chimpanzee)	KRT82	XP_522390.2
	KRT82	XP_001093789.1
Rattus norvegicus (Norway rat)	Krt82	NP_001008803.1
Mus musculus (house mouse)	Krt82	NP_444479.2

Gene Ontology
Bibliography

Related articles in PubMed

Deletion of the epidermis derived laminin ?1 chain leads to defects in the regulation of late hair morphogenesis.
Fleger-Weckmann A, ?st?n Y, Kloepper J, Paus R, Bloch W, Chen ZL, Wegner J, Sorokin L, Langbein L, Eckes B, Zigrino P, Krieg T, Nischt R
Matrix biology : journal of the International Society for Matrix Biology5642-56(2016 Dec)

BRCA1 deficiency in skin epidermis leads to selective loss of hair follicle stem cells and their progeny.
Sotiropoulou PA, Karambelas AE, Debaugnies M, Candi A, Bouwman P, Moers V, Revenco T, Rocha AS, Sekiguchi K, Jonkers J, Blanpain C
Genes & development27(1)39-51(2013 Jan)

Sostdc1 defines the size and number of skin appendage placodes.
N?rhi K, Tummers M, Ahtiainen L, Itoh N, Thesleff I, Mikkola ML
Developmental biology364(2)149-61(2012 Apr)

The disruption of Sox21-mediated hair shaft cuticle differentiation causes cyclic alopecia in mice.
Kiso M, Tanaka S, Saba R, Matsuda S, Shimizu A, Ohyama M, Okano HJ, Shiroishi T, Okano H, Saga Y
Proceedings of the National Academy of Sciences of the United States of America106(23)9292-7(2009 Jun)

Activation of beta-catenin signaling programs embryonic epidermis to hair follicle fate.
Zhang Y, Andl T, Yang SH, Teta M, Liu F, Seykora JT, Tobias JW, Piccolo S, Schmidt-Ullrich R, Nagy A, Taketo MM, Dlugosz AA, Millar SE
Development (Cambridge, England)135(12)2161-72(2008 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Krt82 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Krt82 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu58490	NM_053249.3 Latest version!	Mus musculus keratin 82 (Krt82), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$300.30 $429.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Krt82 ( NM_053249.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_444479.2 Mus musculus keratin 82 (Krt82), mRNA.

Price & Availability↑

CloneID	OMu58490
Clone ID Related Accession (Same CDS sequence)	NM_053249.3
Accession Version	NM_053249.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	keratin, type II cuticular Hb2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC108971.2 and AA607752.1. On Dec 30, 2009 this sequence version replaced NM_053249.2. ##Evidence-Data-START## Transcript exon combination :: BC108971.2, AY028606.1 [ECO:0000332] ##Evidence-Data-END##

ATGTCATGCC GAAATTTCCA GTTGAGCCCC AGATGTGGCA ACCGGAGTTT CAGCTCGTGC 
TCAGCCATCA TGCCCCGGAT GGTCACCCAC TATGAAGTGA GCAAGGCGCC CTGTCGGTCC 
GGGGGTGGCG GGGGCCTCCG AGCCCTGGGT TGCCTTGGCT CTCGGAGCCT GTGCAACGTG 
GGTTTCGGGA GGCCCCGTGT GGCCTCCAGA TGCGGCATGC CAGGCTTCGG GTACCGAGCG 
GGAGCCACCT GTGGATCTTC TGCCTGCATC ACCCCTGTCA CCATCAATGA GAGTCTGCTG 
GTCCCCCTGG AGCTGGAGAT TGACCCGACC GTGCAAAGGG TGAAGAGAGA TGAAAAGGAG 
CAGATTAAGT GCCTGAATAA CCGATTCGCA TCCTTCATCA ACAAGGTGCG GTTCCTGGAG 
CAGAAAAACA AGCTTCTGGA GACCAAGTGG AACTTCATGC AGCAGCAGAG AAGTTGCCAG 
AGCAACATGG AGCCGCTCTT CGAGGGCTAC ATCTGTGCCC TGCGGCGACA GCTGGACTGT 
GTGTCCGGGG ACCACGGCAG ACTAGAGGCC GAGCTCTGCA GTCTCCAGGA GGCGCTGGAG 
GGTTATAAGA AAAAGTACGA AGAGGAGCTG TCTCTGCGTC CCTGTGCTGA GAATGAGTTT 
GTCACCCTGA AGAAGGACGT GGACACAGCC TTCCTGGTGA AGGCTGACCT GGAAACCAAC 
TTGGAGGCTC TAGAACATGA GATTGAGTTC CTGAAAGCCC TGTTTGAGGA GGAGATCGGC 
CTGCTGCAGT CTCAGATCTC AGAGACTTCA GTCATCGTGA AGATGGACAA CAGCCGGGAA 
CTGGATGTGG ACGGCATCGT AGCCGAGATC AAGGCCCAGT ATGACGACAT CGCCAGCCGC 
AGCAAAGCAG AAGCAGAGGC CTGGTACCAG TGCCGGTATG AGGAGCTGAG GTTAACGGCT 
GGGAACCACT GTGACAACCT ACGCAACCGC AAAAATGAGA TCCTGGAAAT GAACAAGCTA 
ATCCAGCGGC TGCAGCAGGA CATTGAGGCA GTCAAAGGCC AGCGATGCAA GCTCGAGGGG 
GCCATTGCCC AGGCAGAGCA GCAGGGAGAG GCCGCCCTCA CTGATGCCAA GTGCAAGCTG 
GCAGGGCTGG AGGAGGCCTT GCAGAAGGCC AAGCAGGACA TGGCCTGCCT GCTCAAGCAG 
TACCAAGAGG TGATGAACTG CAAGCTGGGG CTGGACATCG AGATCGCCAC CTACAGGCGC 
CTGCTGGAGG GCGAGGAGCA CAGGTTGTGT GAAGGCATCG GGCCTGTGAA TATCTCTGTC 
AGCAGCTCCA AAGGTGCCGT ACTCTACGAG CCCTGTGTGA TGGGCACGCC CATGCTGAGG 
ACCGAATACT GCACCGGGAC CACGGGTGTC CTGAGGAACA GTGGGGGCTG CAACGTGGTG 
GGCACCGGTG AACTCTACAT CCCCTGCGAG CCCCAAGGGC TCCTGGGCTG TGGAAATGGG 
CGGAGCTCCA GCATGAAGGT GGGGGTGGGT AGCAATTCGT GCAGCCGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_444479.2
CDS	57..1607
Translation	MSCRNFQLSPRCGNRSFSSCSAIMPRMVTHYEVSKAPCRSGGGGGLRALGCLGSRSLCNVGFGRPRVASRCGMPGFGYRAGATCGSSACITPVTINESLLVPLELEIDPTVQRVKRDEKEQIKCLNNRFASFINKVRFLEQKNKLLETKWNFMQQQRSCQSNMEPLFEGYICALRRQLDCVSGDHGRLEAELCSLQEALEGYKKKYEEELSLRPCAENEFVTLKKDVDTAFLVKADLETNLEALEHEIEFLKALFEEEIGLLQSQISETSVIVKMDNSRELDVDGIVAEIKAQYDDIASRSKAEAEAWYQCRYEELRLTAGNHCDNLRNRKNEILEMNKLIQRLQQDIEAVKGQRCKLEGAIAQAEQQGEAALTDAKCKLAGLEEALQKAKQDMACLLKQYQEVMNCKLGLDIEIATYRRLLEGEEHRLCEGIGPVNISVSSSKGAVLYEPCVMGTPMLRTEYCTGTTGVLRNSGGCNVVGTGELYIPCEPQGLLGCGNGRSSSMKVGVGSNSCSR

Target ORF information:

RefSeq Version	NM_053249.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus keratin 82 (Krt82), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053249.3

ORF Insert Sequence:

ATGTCATGCC GAAATTTCCA GTTGAGCCCC AGATGTGGCA ACCGGAGTTT CAGCTCGTGC 
TCAGCCATCA TGCCCCGGAT GGTCACCCAC TATGAAGTGA GCAAGGCGCC CTGTCGGTCC 
GGGGGTGGCG GGGGCCTCCG AGCCCTGGGT TGCCTTGGCT CTCGGAGCCT GTGCAACGTG 
GGTTTCGGGA GGCCCCGTGT GGCCTCCAGA TGCGGCATGC CAGGCTTCGG GTACCGAGCG 
GGAGCCACCT GTGGATCTTC TGCCTGCATC ACCCCTGTCA CCATCAATGA GAGTCTGCTG 
GTCCCCCTGG AGCTGGAGAT TGACCCGACC GTGCAAAGGG TGAAGAGAGA TGAAAAGGAG 
CAGATTAAGT GCCTGAATAA CCGATTCGCA TCCTTCATCA ACAAGGTGCG GTTCCTGGAG 
CAGAAAAACA AGCTTCTGGA GACCAAGTGG AACTTCATGC AGCAGCAGAG AAGTTGCCAG 
AGCAACATGG AGCCGCTCTT CGAGGGCTAC ATCTGTGCCC TGCGGCGACA GCTGGACTGT 
GTGTCCGGGG ACCACGGCAG ACTAGAGGCC GAGCTCTGCA GTCTCCAGGA GGCGCTGGAG 
GGTTATAAGA AAAAGTACGA AGAGGAGCTG TCTCTGCGTC CCTGTGCTGA GAATGAGTTT 
GTCACCCTGA AGAAGGACGT GGACACAGCC TTCCTGGTGA AGGCTGACCT GGAAACCAAC 
TTGGAGGCTC TAGAACATGA GATTGAGTTC CTGAAAGCCC TGTTTGAGGA GGAGATCGGC 
CTGCTGCAGT CTCAGATCTC AGAGACTTCA GTCATCGTGA AGATGGACAA CAGCCGGGAA 
CTGGATGTGG ACGGCATCGT AGCCGAGATC AAGGCCCAGT ATGACGACAT CGCCAGCCGC 
AGCAAAGCAG AAGCAGAGGC CTGGTACCAG TGCCGGTATG AGGAGCTGAG GTTAACGGCT 
GGGAACCACT GTGACAACCT ACGCAACCGC AAAAATGAGA TCCTGGAAAT GAACAAGCTA 
ATCCAGCGGC TGCAGCAGGA CATTGAGGCA GTCAAAGGCC AGCGATGCAA GCTCGAGGGG 
GCCATTGCCC AGGCAGAGCA GCAGGGAGAG GCCGCCCTCA CTGATGCCAA GTGCAAGCTG 
GCAGGGCTGG AGGAGGCCTT GCAGAAGGCC AAGCAGGACA TGGCCTGCCT GCTCAAGCAG 
TACCAAGAGG TGATGAACTG CAAGCTGGGG CTGGACATCG AGATCGCCAC CTACAGGCGC 
CTGCTGGAGG GCGAGGAGCA CAGGTTGTGT GAAGGCATCG GGCCTGTGAA TATCTCTGTC 
AGCAGCTCCA AAGGTGCCGT ACTCTACGAG CCCTGTGTGA TGGGCACGCC CATGCTGAGG 
ACCGAATACT GCACCGGGAC CACGGGTGTC CTGAGGAACA GTGGGGGCTG CAACGTGGTG 
GGCACCGGTG AACTCTACAT CCCCTGCGAG CCCCAAGGGC TCCTGGGCTG TGGAAATGGG 
CGGAGCTCCA GCATGAAGGT GGGGGTGGGT AGCAATTCGT GCAGCCGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Deletion of the epidermis derived laminin ?1 chain leads to defects in the regulation of late hair morphogenesis.
Matrix biology : journal of the International Society for Matrix Biology5642-56(2016 Dec)
Fleger-Weckmann A,?st?n Y,Kloepper J,Paus R,Bloch W,Chen ZL,Wegner J,Sorokin L,Langbein L,Eckes B,Zigrino P,Krieg T,Nischt R

BRCA1 deficiency in skin epidermis leads to selective loss of hair follicle stem cells and their progeny.
Genes & development27(1)39-51(2013 Jan)
Sotiropoulou PA,Karambelas AE,Debaugnies M,Candi A,Bouwman P,Moers V,Revenco T,Rocha AS,Sekiguchi K,Jonkers J,Blanpain C

Sostdc1 defines the size and number of skin appendage placodes.
Developmental biology364(2)149-61(2012 Apr)
N?rhi K,Tummers M,Ahtiainen L,Itoh N,Thesleff I,Mikkola ML

The disruption of Sox21-mediated hair shaft cuticle differentiation causes cyclic alopecia in mice.
Proceedings of the National Academy of Sciences of the United States of America106(23)9292-7(2009 Jun)
Kiso M,Tanaka S,Saba R,Matsuda S,Shimizu A,Ohyama M,Okano HJ,Shiroishi T,Okano H,Saga Y

Activation of beta-catenin signaling programs embryonic epidermis to hair follicle fate.
Development (Cambridge, England)135(12)2161-72(2008 Jun)
Zhang Y,Andl T,Yang SH,Teta M,Liu F,Seykora JT,Tobias JW,Piccolo S,Schmidt-Ullrich R,Nagy A,Taketo MM,Dlugosz AA,Millar SE

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Krt82 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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