Npffr2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Npffr2
Entrez Gene ID	104443
Full Name	neuropeptide FF receptor 2
Synonyms	Gpr74,HG31,NPFF2
General protein information	Preferred Names neuropeptide FF receptor 2 Names neuropeptide FF receptor 2 G-protein coupled receptor 74 neuropeptide NPFF receptor
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 E1

mRNA and Protein(s)

mRNA	Protein	Name
XM_017320589.1	XP_017176078.1	neuropeptide FF receptor 2 isoform X1
XM_017320589.2	XP_017176078.1	neuropeptide FF receptor 2 isoform X1
NM_133192.3	NP_573455.2	neuropeptide FF receptor 2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Npffr2	NP_076470.1
Anopheles gambiae (African malaria mosquito)	GPRNNA7	XP_314231.4
Homo sapiens (human)	NPFFR2	NP_004876.2
Pan troglodytes (chimpanzee)	NPFFR2	XP_001158763.1
Canis lupus familiaris (dog)	NPFFR2	XP_003639501.1
Bos taurus (cattle)	NPFFR2	XP_005208183.1
Mus musculus (house mouse)	Npffr2	NP_573455.2
Gallus gallus (chicken)	NPFFR2	NP_001029997.2
Xenopus tropicalis (tropical clawed frog)	npffr2	XP_002940397.1
Drosophila melanogaster (fruit fly)	SIFR	NP_001163674.1
Danio rerio (zebrafish)	npffr2.1	NP_001098579.1
Danio rerio (zebrafish)	LOC561565	XP_690069.5

Gene Ontology
Bibliography

Related articles in PubMed

NPFFR2 Activates the HPA Axis and Induces Anxiogenic Effects in Rodents.
Lin YT, Yu YL, Hong WC, Yeh TS, Chen TC, Chen JC
International journal of molecular sciences18(8)(2017 Aug)

Neuropeptide FF increases M2 activation and self-renewal of adipose tissue macrophages.
Waqas SFH, Hoang AC, Lin YT, Ampem G, Azegrouz H, Balogh L, Thur?czy J, Chen JC, Gerling IC, Nam S, Lim JS, Martinez-Iba?ez J, Real JT, Paschke S, Quillet R, Ayachi S, Simonin F, Schneider EM, Brinkman JA, Lamming DW, Seroogy CM, R?szer T
The Journal of clinical investigation127(7)2842-2854(2017 Jun)

Activation of NPFFR2 leads to hyperalgesia through the spinal inflammatory mediator CGRP in mice.
Lin YT, Liu HL, Day YJ, Chang CC, Hsu PH, Chen JC
Experimental neurology29162-73(2017 May)

Pharmacological characterization of the mouse NPFF2 receptor.
Talmont F, Moul?dous L, Piedra-Garcia L, Schmitt M, Bihel F, Bourguignon JJ, Zajac JM, Mollereau C
Peptides31(2)215-20(2010 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results of this study demonstrated that NPFFR2 activation modulates pain transmission by up-regulating the pain mediator CGRP, leading to hyperalgesia.
Title: Activation of NPFFR2 leads to hyperalgesia through the spinal inflammatory mediator CGRP in mice.

Neuro 2A cells endogenously expressed NPFF2 receptor, and the NPFF2 receptor agonist dNPA inhibited cyclic adenosine monophosphate (cAMP) production stimulated by forskolin in Neuro 2A cells.
Title: Activation of NPFF

Results indicate that NPFF signaling through NPFFR2 contributes to diabetic corneal nerve injury recovery and epithelial wound healing. Neuropeptide FF is a potential neuroregenerative factor for diabetic sensory nerve injury.
Title: Neuropeptide FF Promotes Recovery of Corneal Nerve Injury Associated With Hyperglycemia.

kisspeptin can act partly through neuropeptide FF receptors to modulate neuronal activity independent of GPR54 in the mouse brain.
Title: Kisspeptin regulation of arcuate neuron excitability in kisspeptin receptor knockout mice.

mRNA levels of NPFF2 receptor were up-regulated in macrophages when treated with Lipopolysaccharide for 24 to 72 h.
Title: NPFF2 receptor is involved in the modulatory effects of neuropeptide FF for macrophage cell line.

The following Npffr2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Npffr2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu09875	NM_133192.3 Latest version!	Mus musculus neuropeptide FF receptor 2 (Npffr2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OMu09875	XM_017320589.2 Latest version!	Mus musculus neuropeptide FF receptor 2 (Npffr2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu09875	XM_017320589.1	Mus musculus neuropeptide FF receptor 2 (Npffr2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Npffr2 ( NM_133192.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_573455.2 Mus musculus neuropeptide FF receptor 2 (Npffr2), mRNA.

Price & Availability↑

CloneID	OMu09875
Clone ID Related Accession (Same CDS sequence)	XM_017320589.1 , XM_017320589.2 , NM_133192.3
Accession Version	NM_133192.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	neuropeptide FF receptor 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK050939.1. On Apr 6, 2007 this sequence version replaced NM_133192.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK050939.1, BB655171.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164138 [ECO:0000348] ##Evidence-Data-END##

ATGAGCGAGA AATGGGACTC AAACTCTTCA GAAAGCTGGA ATCACATCTG GAGTGGCAAT 
GATACACAGC ATCACTGGTA TTCAGATATC AACATTACCT ATGTGAACTA CTATCTCCAC 
CAGCCCCAAG TGGCAGCTGT CTTCATCAGC TCCTACCTCC TGATCTTTGT CTTGTGCATG 
GTGGGAAATA CTGTCGTTTG CTTTATTGTG ATAAGGAATA GACACATGCA CACAGTCACT 
AATTTCTTCA TCTTAAACCT TGCCATAAGT GATTTACTGG TTGGAATATT CTGTATGCCT 
ATCACATTGC TGGACAACAT CATAGCAGGA TGGCCATTCG GAAGCAGCAT GTGCAAGATC 
AGTGGGCTGG TGCAAGGGAT ATCAGTTGCG GCTTCCGTCT TCACCTTGGT TGCAATAGCT 
GTGGACAGAT TCCGCTGTGT GGTCTACCCC TTTAAGCCAA AGCTCACTGT CAAGACAGCC 
TTTGTCACGA TTGTGATCAT CTGGGGCCTG GCCATCGCCA TTATGACTCC ATCTGCAATA 
ATGTTACATG TACAAGAAGA AAAATACTAC CGTGTGAGAC TCAGCTCCCA CAATAAAACC 
AGCACAGTCT ACTGGTGTCG GGAGGACTGG CCAAGACACG AAATGAGGAG GATCTATACC 
ACGGTGCTAT TTGCCACCAT CTATCTTGCT CCTCTCTCAC TCATTGTTAT CATGTATGCA 
AGGATTGGGG CTTCCCTCTT CAAGACGGCA GCACACTGCA CAGGCAAGCA GCGTCCAGTG 
CAGTGGCATG TATCCAAGAA GAAACAGAAG GTCATCAAGA TGCTGCTGAC TGTGGCCCTC 
CTTTTCATCC TTTCCTGGCT TCCCCTGTGG ACCCTGATGA TGCTCTCAGA CTATACTGAC 
CTGTCTCCTA ACAAACTGCG TATCATCAAC ATCTACATCT ACCCTTTCGC CCACTGGCTC 
GCCTTCTGCA ACAGCAGTGT CAACCCTATT ATTTATGGAT TCTTTAATGA AAATTTTCGC 
AATGGTTTCC AAGATGCTTT CCAGATCTGC CAAAAGAAAG CCAAGCCCCA GGAAGCCTAT 
TCCCTGAGAG CGAAACGCAA CATAGTCATA AACACATCGG GCCTGCTGGT GCAGGAACCG 
GTGTCTCAAA ACCCAGGTGG GGAAAATTTG GGATGTGGAA AAAGTGCAGA CAATCCCACA 
CAGGAATCCT TGATAGAGGA AATGGGAGAA GCTACTAACA GTACTGTGGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_573455.2
CDS	116..1369
Translation	MSEKWDSNSSESWNHIWSGNDTQHHWYSDINITYVNYYLHQPQVAAVFISSYLLIFVLCMVGNTVVCFIVIRNRHMHTVTNFFILNLAISDLLVGIFCMPITLLDNIIAGWPFGSSMCKISGLVQGISVAASVFTLVAIAVDRFRCVVYPFKPKLTVKTAFVTIVIIWGLAIAIMTPSAIMLHVQEEKYYRVRLSSHNKTSTVYWCREDWPRHEMRRIYTTVLFATIYLAPLSLIVIMYARIGASLFKTAAHCTGKQRPVQWHVSKKKQKVIKMLLTVALLFILSWLPLWTLMMLSDYTDLSPNKLRIINIYIYPFAHWLAFCNSSVNPIIYGFFNENFRNGFQDAFQICQKKAKPQEAYSLRAKRNIVINTSGLLVQEPVSQNPGGENLGCGKSADNPTQESLIEEMGEATNSTVA

Target ORF information:

RefSeq Version	NM_133192.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus neuropeptide FF receptor 2 (Npffr2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133192.3

ORF Insert Sequence:

ATGAGCGAGA AATGGGACTC AAACTCTTCA GAAAGCTGGA ATCACATCTG GAGTGGCAAT 
GATACACAGC ATCACTGGTA TTCAGATATC AACATTACCT ATGTGAACTA CTATCTCCAC 
CAGCCCCAAG TGGCAGCTGT CTTCATCAGC TCCTACCTCC TGATCTTTGT CTTGTGCATG 
GTGGGAAATA CTGTCGTTTG CTTTATTGTG ATAAGGAATA GACACATGCA CACAGTCACT 
AATTTCTTCA TCTTAAACCT TGCCATAAGT GATTTACTGG TTGGAATATT CTGTATGCCT 
ATCACATTGC TGGACAACAT CATAGCAGGA TGGCCATTCG GAAGCAGCAT GTGCAAGATC 
AGTGGGCTGG TGCAAGGGAT ATCAGTTGCG GCTTCCGTCT TCACCTTGGT TGCAATAGCT 
GTGGACAGAT TCCGCTGTGT GGTCTACCCC TTTAAGCCAA AGCTCACTGT CAAGACAGCC 
TTTGTCACGA TTGTGATCAT CTGGGGCCTG GCCATCGCCA TTATGACTCC ATCTGCAATA 
ATGTTACATG TACAAGAAGA AAAATACTAC CGTGTGAGAC TCAGCTCCCA CAATAAAACC 
AGCACAGTCT ACTGGTGTCG GGAGGACTGG CCAAGACACG AAATGAGGAG GATCTATACC 
ACGGTGCTAT TTGCCACCAT CTATCTTGCT CCTCTCTCAC TCATTGTTAT CATGTATGCA 
AGGATTGGGG CTTCCCTCTT CAAGACGGCA GCACACTGCA CAGGCAAGCA GCGTCCAGTG 
CAGTGGCATG TATCCAAGAA GAAACAGAAG GTCATCAAGA TGCTGCTGAC TGTGGCCCTC 
CTTTTCATCC TTTCCTGGCT TCCCCTGTGG ACCCTGATGA TGCTCTCAGA CTATACTGAC 
CTGTCTCCTA ACAAACTGCG TATCATCAAC ATCTACATCT ACCCTTTCGC CCACTGGCTC 
GCCTTCTGCA ACAGCAGTGT CAACCCTATT ATTTATGGAT TCTTTAATGA AAATTTTCGC 
AATGGTTTCC AAGATGCTTT CCAGATCTGC CAAAAGAAAG CCAAGCCCCA GGAAGCCTAT 
TCCCTGAGAG CGAAACGCAA CATAGTCATA AACACATCGG GCCTGCTGGT GCAGGAACCG 
GTGTCTCAAA ACCCAGGTGG GGAAAATTTG GGATGTGGAA AAAGTGCAGA CAATCCCACA 
CAGGAATCCT TGATAGAGGA AATGGGAGAA GCTACTAACA GTACTGTGGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Npffr2 ( XM_017320589.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176078.1 Mus musculus neuropeptide FF receptor 2 (Npffr2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu09875
Clone ID Related Accession (Same CDS sequence)	XM_017320589.1 , XM_017320589.2 , NM_133192.3
Accession Version	XM_017320589.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neuropeptide FF receptor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGA AATGGGACTC AAACTCTTCA GAAAGCTGGA ATCACATCTG GAGTGGCAAT 
GATACACAGC ATCACTGGTA TTCAGATATC AACATTACCT ATGTGAACTA CTATCTCCAC 
CAGCCCCAAG TGGCAGCTGT CTTCATCAGC TCCTACCTCC TGATCTTTGT CTTGTGCATG 
GTGGGAAATA CTGTCGTTTG CTTTATTGTG ATAAGGAATA GACACATGCA CACAGTCACT 
AATTTCTTCA TCTTAAACCT TGCCATAAGT GATTTACTGG TTGGAATATT CTGTATGCCT 
ATCACATTGC TGGACAACAT CATAGCAGGA TGGCCATTCG GAAGCAGCAT GTGCAAGATC 
AGTGGGCTGG TGCAAGGGAT ATCAGTTGCG GCTTCCGTCT TCACCTTGGT TGCAATAGCT 
GTGGACAGAT TCCGCTGTGT GGTCTACCCC TTTAAGCCAA AGCTCACTGT CAAGACAGCC 
TTTGTCACGA TTGTGATCAT CTGGGGCCTG GCCATCGCCA TTATGACTCC ATCTGCAATA 
ATGTTACATG TACAAGAAGA AAAATACTAC CGTGTGAGAC TCAGCTCCCA CAATAAAACC 
AGCACAGTCT ACTGGTGTCG GGAGGACTGG CCAAGACACG AAATGAGGAG GATCTATACC 
ACGGTGCTAT TTGCCACCAT CTATCTTGCT CCTCTCTCAC TCATTGTTAT CATGTATGCA 
AGGATTGGGG CTTCCCTCTT CAAGACGGCA GCACACTGCA CAGGCAAGCA GCGTCCAGTG 
CAGTGGCATG TATCCAAGAA GAAACAGAAG GTCATCAAGA TGCTGCTGAC TGTGGCCCTC 
CTTTTCATCC TTTCCTGGCT TCCCCTGTGG ACCCTGATGA TGCTCTCAGA CTATACTGAC 
CTGTCTCCTA ACAAACTGCG TATCATCAAC ATCTACATCT ACCCTTTCGC CCACTGGCTC 
GCCTTCTGCA ACAGCAGTGT CAACCCTATT ATTTATGGAT TCTTTAATGA AAATTTTCGC 
AATGGTTTCC AAGATGCTTT CCAGATCTGC CAAAAGAAAG CCAAGCCCCA GGAAGCCTAT 
TCCCTGAGAG CGAAACGCAA CATAGTCATA AACACATCGG GCCTGCTGGT GCAGGAACCG 
GTGTCTCAAA ACCCAGGTGG GGAAAATTTG GGATGTGGAA AAAGTGCAGA CAATCCCACA 
CAGGAATCCT TGATAGAGGA AATGGGAGAA GCTACTAACA GTACTGTGGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176078.1
CDS	213..1466
Translation	MSEKWDSNSSESWNHIWSGNDTQHHWYSDINITYVNYYLHQPQVAAVFISSYLLIFVLCMVGNTVVCFIVIRNRHMHTVTNFFILNLAISDLLVGIFCMPITLLDNIIAGWPFGSSMCKISGLVQGISVAASVFTLVAIAVDRFRCVVYPFKPKLTVKTAFVTIVIIWGLAIAIMTPSAIMLHVQEEKYYRVRLSSHNKTSTVYWCREDWPRHEMRRIYTTVLFATIYLAPLSLIVIMYARIGASLFKTAAHCTGKQRPVQWHVSKKKQKVIKMLLTVALLFILSWLPLWTLMMLSDYTDLSPNKLRIINIYIYPFAHWLAFCNSSVNPIIYGFFNENFRNGFQDAFQICQKKAKPQEAYSLRAKRNIVINTSGLLVQEPVSQNPGGENLGCGKSADNPTQESLIEEMGEATNSTVA

Target ORF information:

RefSeq Version	XM_017320589.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neuropeptide FF receptor 2 (Npffr2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320589.1

ORF Insert Sequence:

ATGAGCGAGA AATGGGACTC AAACTCTTCA GAAAGCTGGA ATCACATCTG GAGTGGCAAT 
GATACACAGC ATCACTGGTA TTCAGATATC AACATTACCT ATGTGAACTA CTATCTCCAC 
CAGCCCCAAG TGGCAGCTGT CTTCATCAGC TCCTACCTCC TGATCTTTGT CTTGTGCATG 
GTGGGAAATA CTGTCGTTTG CTTTATTGTG ATAAGGAATA GACACATGCA CACAGTCACT 
AATTTCTTCA TCTTAAACCT TGCCATAAGT GATTTACTGG TTGGAATATT CTGTATGCCT 
ATCACATTGC TGGACAACAT CATAGCAGGA TGGCCATTCG GAAGCAGCAT GTGCAAGATC 
AGTGGGCTGG TGCAAGGGAT ATCAGTTGCG GCTTCCGTCT TCACCTTGGT TGCAATAGCT 
GTGGACAGAT TCCGCTGTGT GGTCTACCCC TTTAAGCCAA AGCTCACTGT CAAGACAGCC 
TTTGTCACGA TTGTGATCAT CTGGGGCCTG GCCATCGCCA TTATGACTCC ATCTGCAATA 
ATGTTACATG TACAAGAAGA AAAATACTAC CGTGTGAGAC TCAGCTCCCA CAATAAAACC 
AGCACAGTCT ACTGGTGTCG GGAGGACTGG CCAAGACACG AAATGAGGAG GATCTATACC 
ACGGTGCTAT TTGCCACCAT CTATCTTGCT CCTCTCTCAC TCATTGTTAT CATGTATGCA 
AGGATTGGGG CTTCCCTCTT CAAGACGGCA GCACACTGCA CAGGCAAGCA GCGTCCAGTG 
CAGTGGCATG TATCCAAGAA GAAACAGAAG GTCATCAAGA TGCTGCTGAC TGTGGCCCTC 
CTTTTCATCC TTTCCTGGCT TCCCCTGTGG ACCCTGATGA TGCTCTCAGA CTATACTGAC 
CTGTCTCCTA ACAAACTGCG TATCATCAAC ATCTACATCT ACCCTTTCGC CCACTGGCTC 
GCCTTCTGCA ACAGCAGTGT CAACCCTATT ATTTATGGAT TCTTTAATGA AAATTTTCGC 
AATGGTTTCC AAGATGCTTT CCAGATCTGC CAAAAGAAAG CCAAGCCCCA GGAAGCCTAT 
TCCCTGAGAG CGAAACGCAA CATAGTCATA AACACATCGG GCCTGCTGGT GCAGGAACCG 
GTGTCTCAAA ACCCAGGTGG GGAAAATTTG GGATGTGGAA AAAGTGCAGA CAATCCCACA 
CAGGAATCCT TGATAGAGGA AATGGGAGAA GCTACTAACA GTACTGTGGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Npffr2 ( XM_017320589.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176078.1 Mus musculus neuropeptide FF receptor 2 (Npffr2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu09875
Clone ID Related Accession (Same CDS sequence)	XM_017320589.1 , XM_017320589.2 , NM_133192.3
Accession Version	XM_017320589.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neuropeptide FF receptor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017320589.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGA AATGGGACTC AAACTCTTCA GAAAGCTGGA ATCACATCTG GAGTGGCAAT 
GATACACAGC ATCACTGGTA TTCAGATATC AACATTACCT ATGTGAACTA CTATCTCCAC 
CAGCCCCAAG TGGCAGCTGT CTTCATCAGC TCCTACCTCC TGATCTTTGT CTTGTGCATG 
GTGGGAAATA CTGTCGTTTG CTTTATTGTG ATAAGGAATA GACACATGCA CACAGTCACT 
AATTTCTTCA TCTTAAACCT TGCCATAAGT GATTTACTGG TTGGAATATT CTGTATGCCT 
ATCACATTGC TGGACAACAT CATAGCAGGA TGGCCATTCG GAAGCAGCAT GTGCAAGATC 
AGTGGGCTGG TGCAAGGGAT ATCAGTTGCG GCTTCCGTCT TCACCTTGGT TGCAATAGCT 
GTGGACAGAT TCCGCTGTGT GGTCTACCCC TTTAAGCCAA AGCTCACTGT CAAGACAGCC 
TTTGTCACGA TTGTGATCAT CTGGGGCCTG GCCATCGCCA TTATGACTCC ATCTGCAATA 
ATGTTACATG TACAAGAAGA AAAATACTAC CGTGTGAGAC TCAGCTCCCA CAATAAAACC 
AGCACAGTCT ACTGGTGTCG GGAGGACTGG CCAAGACACG AAATGAGGAG GATCTATACC 
ACGGTGCTAT TTGCCACCAT CTATCTTGCT CCTCTCTCAC TCATTGTTAT CATGTATGCA 
AGGATTGGGG CTTCCCTCTT CAAGACGGCA GCACACTGCA CAGGCAAGCA GCGTCCAGTG 
CAGTGGCATG TATCCAAGAA GAAACAGAAG GTCATCAAGA TGCTGCTGAC TGTGGCCCTC 
CTTTTCATCC TTTCCTGGCT TCCCCTGTGG ACCCTGATGA TGCTCTCAGA CTATACTGAC 
CTGTCTCCTA ACAAACTGCG TATCATCAAC ATCTACATCT ACCCTTTCGC CCACTGGCTC 
GCCTTCTGCA ACAGCAGTGT CAACCCTATT ATTTATGGAT TCTTTAATGA AAATTTTCGC 
AATGGTTTCC AAGATGCTTT CCAGATCTGC CAAAAGAAAG CCAAGCCCCA GGAAGCCTAT 
TCCCTGAGAG CGAAACGCAA CATAGTCATA AACACATCGG GCCTGCTGGT GCAGGAACCG 
GTGTCTCAAA ACCCAGGTGG GGAAAATTTG GGATGTGGAA AAAGTGCAGA CAATCCCACA 
CAGGAATCCT TGATAGAGGA AATGGGAGAA GCTACTAACA GTACTGTGGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176078.1
CDS	353..1606
Translation	MSEKWDSNSSESWNHIWSGNDTQHHWYSDINITYVNYYLHQPQVAAVFISSYLLIFVLCMVGNTVVCFIVIRNRHMHTVTNFFILNLAISDLLVGIFCMPITLLDNIIAGWPFGSSMCKISGLVQGISVAASVFTLVAIAVDRFRCVVYPFKPKLTVKTAFVTIVIIWGLAIAIMTPSAIMLHVQEEKYYRVRLSSHNKTSTVYWCREDWPRHEMRRIYTTVLFATIYLAPLSLIVIMYARIGASLFKTAAHCTGKQRPVQWHVSKKKQKVIKMLLTVALLFILSWLPLWTLMMLSDYTDLSPNKLRIINIYIYPFAHWLAFCNSSVNPIIYGFFNENFRNGFQDAFQICQKKAKPQEAYSLRAKRNIVINTSGLLVQEPVSQNPGGENLGCGKSADNPTQESLIEEMGEATNSTVA

Target ORF information:

RefSeq Version	XM_017320589.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus neuropeptide FF receptor 2 (Npffr2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320589.2

ORF Insert Sequence:

ATGAGCGAGA AATGGGACTC AAACTCTTCA GAAAGCTGGA ATCACATCTG GAGTGGCAAT 
GATACACAGC ATCACTGGTA TTCAGATATC AACATTACCT ATGTGAACTA CTATCTCCAC 
CAGCCCCAAG TGGCAGCTGT CTTCATCAGC TCCTACCTCC TGATCTTTGT CTTGTGCATG 
GTGGGAAATA CTGTCGTTTG CTTTATTGTG ATAAGGAATA GACACATGCA CACAGTCACT 
AATTTCTTCA TCTTAAACCT TGCCATAAGT GATTTACTGG TTGGAATATT CTGTATGCCT 
ATCACATTGC TGGACAACAT CATAGCAGGA TGGCCATTCG GAAGCAGCAT GTGCAAGATC 
AGTGGGCTGG TGCAAGGGAT ATCAGTTGCG GCTTCCGTCT TCACCTTGGT TGCAATAGCT 
GTGGACAGAT TCCGCTGTGT GGTCTACCCC TTTAAGCCAA AGCTCACTGT CAAGACAGCC 
TTTGTCACGA TTGTGATCAT CTGGGGCCTG GCCATCGCCA TTATGACTCC ATCTGCAATA 
ATGTTACATG TACAAGAAGA AAAATACTAC CGTGTGAGAC TCAGCTCCCA CAATAAAACC 
AGCACAGTCT ACTGGTGTCG GGAGGACTGG CCAAGACACG AAATGAGGAG GATCTATACC 
ACGGTGCTAT TTGCCACCAT CTATCTTGCT CCTCTCTCAC TCATTGTTAT CATGTATGCA 
AGGATTGGGG CTTCCCTCTT CAAGACGGCA GCACACTGCA CAGGCAAGCA GCGTCCAGTG 
CAGTGGCATG TATCCAAGAA GAAACAGAAG GTCATCAAGA TGCTGCTGAC TGTGGCCCTC 
CTTTTCATCC TTTCCTGGCT TCCCCTGTGG ACCCTGATGA TGCTCTCAGA CTATACTGAC 
CTGTCTCCTA ACAAACTGCG TATCATCAAC ATCTACATCT ACCCTTTCGC CCACTGGCTC 
GCCTTCTGCA ACAGCAGTGT CAACCCTATT ATTTATGGAT TCTTTAATGA AAATTTTCGC 
AATGGTTTCC AAGATGCTTT CCAGATCTGC CAAAAGAAAG CCAAGCCCCA GGAAGCCTAT 
TCCCTGAGAG CGAAACGCAA CATAGTCATA AACACATCGG GCCTGCTGGT GCAGGAACCG 
GTGTCTCAAA ACCCAGGTGG GGAAAATTTG GGATGTGGAA AAAGTGCAGA CAATCCCACA 
CAGGAATCCT TGATAGAGGA AATGGGAGAA GCTACTAACA GTACTGTGGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

NPFFR2 Activates the HPA Axis and Induces Anxiogenic Effects in Rodents.
International journal of molecular sciences18(8)(2017 Aug)
Lin YT,Yu YL,Hong WC,Yeh TS,Chen TC,Chen JC

Neuropeptide FF increases M2 activation and self-renewal of adipose tissue macrophages.
The Journal of clinical investigation127(7)2842-2854(2017 Jun)
Waqas SFH,Hoang AC,Lin YT,Ampem G,Azegrouz H,Balogh L,Thur?czy J,Chen JC,Gerling IC,Nam S,Lim JS,Martinez-Iba?ez J,Real JT,Paschke S,Quillet R,Ayachi S,Simonin F,Schneider EM,Brinkman JA,Lamming DW,Seroogy CM,R?szer T

Activation of NPFFR2 leads to hyperalgesia through the spinal inflammatory mediator CGRP in mice.
Experimental neurology29162-73(2017 May)
Lin YT,Liu HL,Day YJ,Chang CC,Hsu PH,Chen JC

Pharmacological characterization of the mouse NPFF2 receptor.
Peptides31(2)215-20(2010 Feb)
Talmont F,Moul?dous L,Piedra-Garcia L,Schmitt M,Bihel F,Bourguignon JJ,Zajac JM,Mollereau C

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Npffr2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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