Blmh cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Blmh
Entrez Gene ID	104184
Full Name	bleomycin hydrolase
Synonyms	AI035728,Bh,Bmh
General protein information	Preferred Names bleomycin hydrolase Names bleomycin hydrolase BLM hydrolase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 B5
Summary	The encoded protein is a cytoplasmic cysteine peptidase involved in inactivation of bleomycin, a glycopeptide which is a component of combination chemotherapy regimens for cancer. This encoded enzyme is highly conserved, and it contains the signature active site residues of cysteine protease papain superfamily enzymes. It is postulated that this enzyme has protective effects against bleomycin-induced pulmonary fibrosis and bleomycin tumor resistance. [provided by RefSeq, Jan 2010].

mRNA and Protein(s)

mRNA	Protein	Name
NM_178645.4	NP_848760.1	bleomycin hydrolase

Pathways from BioSystems

Homologies

Bos taurus (cattle)	BLMH	XP_002695724.3
Mus musculus (house mouse)	Blmh	NP_848760.1
Rattus norvegicus (Norway rat)	Blmh	NP_001029335.1
Drosophila melanogaster (fruit fly)	CG1440	NP_001096907.1
Eremothecium gossypii	AGOS_ABL204W	NP_982743.1
Neurospora crassa	NCU08333	XP_965236.2
	BLMH	XP_002800396.1
Canis lupus familiaris (dog)	BLMH	XP_537755.3
Gallus gallus (chicken)	BLMH	NP_990435.1
Homo sapiens (human)	BLMH	NP_000377.1
Pan troglodytes (chimpanzee)	BLMH	XP_511383.3
Danio rerio (zebrafish)	blmh	NP_956312.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001341	XP_321804.3
Saccharomyces cerevisiae (baker's yeast)	LAP3	NP_014160.2
Magnaporthe oryzae (rice blast fungus)	MGG_02695	XP_003721070.1
Xenopus tropicalis (tropical clawed frog)	blmh	NP_001006900.1

Gene Ontology
Bibliography

Related articles in PubMed

Methionine-induced hyperhomocysteinemia and bleomycin hydrolase deficiency alter the expression of mouse kidney proteins involved in renal disease.
Suszy?ska-Zajczyk J, Utyro O, Jakubowski H
Molecular genetics and metabolism112(4)339-46(2014 Aug)

Metabolism and neurotoxicity of homocysteine thiolactone in mice: protective role of bleomycin hydrolase.
Borowczyk K, Tiso?czyk J, Jakubowski H
Amino acids43(3)1339-48(2012 Sep)

Analysis of the role of bleomycin hydrolase in antigen presentation and the generation of CD8 T cell responses.
Towne CF, York IA, Watkin LB, Lazo JS, Rock KL
Journal of immunology (Baltimore, Md. : 1950)178(11)6923-30(2007 Jun)

Astrogliosis and behavioral changes in mice lacking the neutral cysteine protease bleomycin hydrolase.
Montoya SE, Thiels E, Card JP, Lazo JS
Neuroscience146(3)890-900(2007 May)

Bleomycin hydrolase and a genetic locus within the MHC affect risk for pulmonary fibrosis in mice.
Haston CK, Wang M, Dejournett RE, Zhou X, Ni D, Gu X, King TM, Weil MM, Newman RA, Amos CI, Travis EL
Human molecular genetics11(16)1855-63(2002 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Blmh interacts with diverse cellular processes--lipoprotein, amino acid and protein, carbohydrate, and energy metabolisms, detoxification, antioxidant defenses--that are essential for normal kidney homeostasis.
Title: Methionine-induced hyperhomocysteinemia and bleomycin hydrolase deficiency alter the expression of mouse kidney proteins involved in renal disease.

Bleomycin hydrolase protects against neurodegeneration associated with elevated homocysteine thiolactone levels in hyperhomocysteinemia and Alzheimer's disease.
Title: Metabolism and neurotoxicity of homocysteine thiolactone in mice: protective role of bleomycin hydrolase.

BH does not play a major role either in generating or destroying class I major histocompatibility antigen (MHC)-presented peptides that bind to the MHC in living cells. These results point to redundant functions between peptidases.
Title: Analysis of the role of bleomycin hydrolase in antigen presentation and the generation of CD8 T cell responses.

bleomycin hydrolase functions as an MHC class I epitope-processing protease
Title: Bleomycin hydrolase and a genetic locus within the MHC affect risk for pulmonary fibrosis in mice.

The following Blmh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Blmh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu15360	NM_178645.4 Latest version!	Mus musculus bleomycin hydrolase (Blmh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$272.30 $389.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Blmh ( NM_178645.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_848760.1 Mus musculus bleomycin hydrolase (Blmh), mRNA.

Price & Availability↑

CloneID	OMu15360
Clone ID Related Accession (Same CDS sequence)	NM_178645.4
Accession Version	NM_178645.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	bleomycin hydrolase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY087076.1, AK049461.1 and BY643607.1. On Jan 13, 2010 this sequence version replaced NM_178645.3. ##Evidence-Data-START## Transcript exon combination :: AK049461.1, BC027403.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACAACG CAGGACTAAA TTCGGAGAAG GTATCTGCTT TGATCCAGAA ACTAAATTCT 
GACCCTCAGT TCGTACTTGC CCAGAATGTC GGGACCACCC ACGATCTGCT GGACATATGT 
CTGAGGAGGG CCACGGTCCA GGGTGCTCAG CATGTGTTTC AACACGTTGT GCCTCAGGAA 
GGCAAGCCAG TCACCAACCA GAAGAGCTCT GGACGATGCT GGATCTTTTC TTGTTTGAAT 
GTTATGAGAC TTCCATTCAT GAAAAAATTT AACATTGAAG AATTTGAGTT TAGTCAATCT 
TACCTGTTTT TTTGGGACAA GGTTGAACGC TGTTATTTCT TCTTGAATGC TTTTGTGGAC 
ACAGCTCAGA AAAAGGAGCC TGAAGATGGG AGACTGGTGC AGTATTTGCT TATGAACCCT 
ACAAATGATG GTGGACAGTG GGATATGCTT GTCAATATTG TTGAAAAATA CGGTGTTGTT 
CCTAAGAAAT GCTTTCCCGA ATCGCATACG ACAGAGGCTA CCAGAAGAAT GAATGATATT 
CTGAATCACA AGATGAGAGA ATTCTGTATA CGGCTTCGGA ACCTGGTACA CAGTGGAGCC 
ACCAAAGGGG AGATCTCATC TACACAAGAT GCCATGATGG AGGAGATATT CAGAGTGGTG 
TGCATCTGTT TGGGCAACCC ACCAGAGACC TTCACCTGGG AGTACCGAGA CAAAGATAAA 
AATTACCACA AGATTGGCCC CATAACACCC TTGCAGTTTT ACAAGGAGCA CGTGAAGCCG 
CTCTTCAATA TGGAAGATAA GATTTGTTTT GTGAATGACC CACGGCCCCA GCACAAGTAC 
AATAAATTGT ACACAGTAGA CTACTTGAGC AATATGGTTG GAGGGAGAAA AACGCTGTAT 
AACAACCAGC CCATTGACTT CTTGAAAAAG ATGGTTGCTG CCTCCATCAA AGATGGAGAG 
GCTGTGTGGT TTGGCTGTGA TGTTGGAAAA CACTTCAATG GCAAGCTGGG CCTCAGTGAC 
ATGAATGTCT ATGACCATGA GTTAGTGTTC GGTGTCTCCT TGAAGAACAT GAATAAAGCT 
GAGAGGTTGG CTTTTGGTGA GTCACTCATG ACCCACGCCA TGACCTTCAC TGCTGTCTCA 
GAGAAGGATA ATCAGGAAGG TACTTTTGTG AAATGGAGAG TAGAGAATTC ATGGGGTGAA 
GACCACGGCC ACAAAGGCTA CCTGTGCATG ACCGATGAGT GGTTCTCGGA GTACGTCTAC 
GAGGTGGTGG TGGACAAGAA GCATGTCCCG GAAGAGGTGC TGGCTGTGTT AGAGCAGGAG 
CCCATTGTCC TGCCGGCATG GGACCCCATG GGGGCTTTGG CTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_848760.1
CDS	226..1593
Translation	MNNAGLNSEKVSALIQKLNSDPQFVLAQNVGTTHDLLDICLRRATVQGAQHVFQHVVPQEGKPVTNQKSSGRCWIFSCLNVMRLPFMKKFNIEEFEFSQSYLFFWDKVERCYFFLNAFVDTAQKKEPEDGRLVQYLLMNPTNDGGQWDMLVNIVEKYGVVPKKCFPESHTTEATRRMNDILNHKMREFCIRLRNLVHSGATKGEISSTQDAMMEEIFRVVCICLGNPPETFTWEYRDKDKNYHKIGPITPLQFYKEHVKPLFNMEDKICFVNDPRPQHKYNKLYTVDYLSNMVGGRKTLYNNQPIDFLKKMVAASIKDGEAVWFGCDVGKHFNGKLGLSDMNVYDHELVFGVSLKNMNKAERLAFGESLMTHAMTFTAVSEKDNQEGTFVKWRVENSWGEDHGHKGYLCMTDEWFSEYVYEVVVDKKHVPEEVLAVLEQEPIVLPAWDPMGALAE

Target ORF information:

RefSeq Version	NM_178645.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus bleomycin hydrolase (Blmh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178645.4

ORF Insert Sequence:

ATGAACAACG CAGGACTAAA TTCGGAGAAG GTATCTGCTT TGATCCAGAA ACTAAATTCT 
GACCCTCAGT TCGTACTTGC CCAGAATGTC GGGACCACCC ACGATCTGCT GGACATATGT 
CTGAGGAGGG CCACGGTCCA GGGTGCTCAG CATGTGTTTC AACACGTTGT GCCTCAGGAA 
GGCAAGCCAG TCACCAACCA GAAGAGCTCT GGACGATGCT GGATCTTTTC TTGTTTGAAT 
GTTATGAGAC TTCCATTCAT GAAAAAATTT AACATTGAAG AATTTGAGTT TAGTCAATCT 
TACCTGTTTT TTTGGGACAA GGTTGAACGC TGTTATTTCT TCTTGAATGC TTTTGTGGAC 
ACAGCTCAGA AAAAGGAGCC TGAAGATGGG AGACTGGTGC AGTATTTGCT TATGAACCCT 
ACAAATGATG GTGGACAGTG GGATATGCTT GTCAATATTG TTGAAAAATA CGGTGTTGTT 
CCTAAGAAAT GCTTTCCCGA ATCGCATACG ACAGAGGCTA CCAGAAGAAT GAATGATATT 
CTGAATCACA AGATGAGAGA ATTCTGTATA CGGCTTCGGA ACCTGGTACA CAGTGGAGCC 
ACCAAAGGGG AGATCTCATC TACACAAGAT GCCATGATGG AGGAGATATT CAGAGTGGTG 
TGCATCTGTT TGGGCAACCC ACCAGAGACC TTCACCTGGG AGTACCGAGA CAAAGATAAA 
AATTACCACA AGATTGGCCC CATAACACCC TTGCAGTTTT ACAAGGAGCA CGTGAAGCCG 
CTCTTCAATA TGGAAGATAA GATTTGTTTT GTGAATGACC CACGGCCCCA GCACAAGTAC 
AATAAATTGT ACACAGTAGA CTACTTGAGC AATATGGTTG GAGGGAGAAA AACGCTGTAT 
AACAACCAGC CCATTGACTT CTTGAAAAAG ATGGTTGCTG CCTCCATCAA AGATGGAGAG 
GCTGTGTGGT TTGGCTGTGA TGTTGGAAAA CACTTCAATG GCAAGCTGGG CCTCAGTGAC 
ATGAATGTCT ATGACCATGA GTTAGTGTTC GGTGTCTCCT TGAAGAACAT GAATAAAGCT 
GAGAGGTTGG CTTTTGGTGA GTCACTCATG ACCCACGCCA TGACCTTCAC TGCTGTCTCA 
GAGAAGGATA ATCAGGAAGG TACTTTTGTG AAATGGAGAG TAGAGAATTC ATGGGGTGAA 
GACCACGGCC ACAAAGGCTA CCTGTGCATG ACCGATGAGT GGTTCTCGGA GTACGTCTAC 
GAGGTGGTGG TGGACAAGAA GCATGTCCCG GAAGAGGTGC TGGCTGTGTT AGAGCAGGAG 
CCCATTGTCC TGCCGGCATG GGACCCCATG GGGGCTTTGG CTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Methionine-induced hyperhomocysteinemia and bleomycin hydrolase deficiency alter the expression of mouse kidney proteins involved in renal disease.
Molecular genetics and metabolism112(4)339-46(2014 Aug)
Suszy?ska-Zajczyk J,Utyro O,Jakubowski H

Metabolism and neurotoxicity of homocysteine thiolactone in mice: protective role of bleomycin hydrolase.
Amino acids43(3)1339-48(2012 Sep)
Borowczyk K,Tiso?czyk J,Jakubowski H

Analysis of the role of bleomycin hydrolase in antigen presentation and the generation of CD8 T cell responses.
Journal of immunology (Baltimore, Md. : 1950)178(11)6923-30(2007 Jun)
Towne CF,York IA,Watkin LB,Lazo JS,Rock KL

Astrogliosis and behavioral changes in mice lacking the neutral cysteine protease bleomycin hydrolase.
Neuroscience146(3)890-900(2007 May)
Montoya SE,Thiels E,Card JP,Lazo JS

Bleomycin hydrolase and a genetic locus within the MHC affect risk for pulmonary fibrosis in mice.
Human molecular genetics11(16)1855-63(2002 Aug)
Haston CK,Wang M,Dejournett RE,Zhou X,Ni D,Gu X,King TM,Weil MM,Newman RA,Amos CI,Travis EL

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Blmh cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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