Cbx4 cDNA ORF clone, Mus caroli(Ryukyu mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cbx4
Entrez Gene ID	110305695
Full Name	chromobox 4
Gene Type	protein-coding
Organism	Mus caroli(Ryukyu mouse)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021177758.1	XP_021033417.1	E3 SUMO-protein ligase CBX4
XM_021177758.2	XP_021033417.1	E3 SUMO-protein ligase CBX4

Pathways from BioSystems
Gene Ontology

The following Cbx4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cbx4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMv27678	XM_021177758.2 Latest version!	Mus caroli chromobox 4 (Cbx4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
OMv27678	XM_021177758.1	Mus caroli chromobox 4 (Cbx4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cbx4 ( XM_021177758.1 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021033417.1 Mus caroli chromobox 4 (Cbx4), mRNA.

Price & Availability↑

CloneID	OMv27678
Clone ID Related Accession (Same CDS sequence)	XM_021177758.1 , XM_021177758.2
Accession Version	XM_021177758.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-20
Organism	Mus caroli(Ryukyu mouse)
Product	E3 SUMO-protein ligase CBX4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034580.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus caroli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGC CAGCTGTTGG CGAGCACGTC TTCGCGGTGG AGAGCATCGA AAAGAAGCGG 
ATCCGCAAGG GCAGAGTGGA GTATCTGGTG AAATGGAGAG GCTGGTCCCC CAAATATAAC 
ACGTGGGAAC CAGAGGAGAA CATCTTGGAT CCCCGTCTGC TGATCGCCTT CCAGAACAGG 
GAAAGGCAGG AGCAGCTGAT GGGATATCGC AAGAGAGGGC CCAAGCCCAA ACCGCTGGTG 
GTGCAGGTGC CCACCTTTGC CCGACGCTCC AATGTCCTGA CTGGGCTTCA AGACTCCTCC 
GCTGACAACC GTGCCAAGCT GGAGTTGGGC ACACAGGGCA AGGGCCAGGG GCACCAGTAT 
GAGCTCAACA GCAAGAAGCA CCATCAGTAC CAACCACATA GCAAGGAGAG GTCCGGTAAG 
CCGCCCCCGC CGGGGAAGAG CGGCAAGTAT TATTATCAGC TAAATAGCAA AAAGCACCAC 
CCCTACCAGC CAGACCCCAA AATGTACGAC CTGCAGTACC AGGGCGGCCA CAAGGAGGCA 
CCCAGCCCCA CCTGCCCAGA CCTGGGCACC AAGAGCCACC CTCCTGACAA GTGGGCCCAC 
GGAGCTGCAG CCAAAGGCTA CCTCGGAGCC GTGAAGCCCT TGGGGGGAGG GGCAGGAGCT 
CCAGGCAAGG GCTCGGAAAA GGGCCCCCCC AATGGTATGA CACCAGCCCC TAAGGAGGCA 
GTGACCGGTA ACGGTATTGG GGGCAAGATG AAAATTGTCA AGAACAAGAA CAAGAACGGG 
CGCATCGTGA TCGTTATGAG CAAGTACATG GAGAACGGCA TGCAAGCAGT GAAAATCAAG 
TCGGGCGAGG CCGGCGAGGG CGAGGCGCGC TCCCCCAGCC ATAAGAAACG TGCTGCCGAG 
GAGCGCCATC CCCAGAGCGA CAGGACTTTT AAAAAGGCAG CCGGTGCTTC TGAGGAGAAG 
AAAGCCGAAG TGCCCTGCAA ACGCAGGGAG GAGGAGGCTC TGGTGTCGGG GGACGCTCAG 
CCCCAGGACC TAGGGTCTAG AAAGCTCTCC CCGACCAAGG AGGCCTTTGG TGAGCAACCC 
CTACAGCTCA CCACCAAGCC AGACCTGCTG GCCTGGGACC CAGCCAGGAG CTCACACCCG 
CCCGCCCACC ACCACCATCA CCACCACCAT CACCACCACC ACCATACGGT GGGCCTGAAC 
CTCTCCCATG CGCGCAAGCG CTGCCTCTCC GAGACCCATG GCGAGCGTGA GCCCTGTAAG 
AAGCGGTTGA CCGCCCGCAG CATTAGCACG CCGACCTGCC TGGGGGGCAG CCCCGTCTCC 
GAGCACCCTG CCAACGTATC CCCCACCGCG GCCTCTCTGC CACAGCCCGA GGTCATCCTT 
TTGGACTCAG ACCTGGATGA ACCCATAGAC TTGCGCTGTG TCAAGATGCG CAGCGATGCC 
GGGGAACCGC CCAGCACCCT CCAGGTGAAG CCCGAGGCTC CGGCGGTGGC GGCAGTGGTG 
GCGCCGGCCA CCGCCTCGGA GAAACCTCCC GCTGAGGCCC AGGAGGAGCC AGTGGAGCCC 
CTGAGTGAGT TCAAGCCCTT CTTTGGGAAT ATAATTATCA CTGACGTCAC GGCAAACTGC 
CTTACCGTCA CTTTCAAGGA ATACGTGACC GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021033417.1
CDS	225..1880
Translation	MELPAVGEHVFAVESIEKKRIRKGRVEYLVKWRGWSPKYNTWEPEENILDPRLLIAFQNRERQEQLMGYRKRGPKPKPLVVQVPTFARRSNVLTGLQDSSADNRAKLELGTQGKGQGHQYELNSKKHHQYQPHSKERSGKPPPPGKSGKYYYQLNSKKHHPYQPDPKMYDLQYQGGHKEAPSPTCPDLGTKSHPPDKWAHGAAAKGYLGAVKPLGGGAGAPGKGSEKGPPNGMTPAPKEAVTGNGIGGKMKIVKNKNKNGRIVIVMSKYMENGMQAVKIKSGEAGEGEARSPSHKKRAAEERHPQSDRTFKKAAGASEEKKAEVPCKRREEEALVSGDAQPQDLGSRKLSPTKEAFGEQPLQLTTKPDLLAWDPARSSHPPAHHHHHHHHHHHHHTVGLNLSHARKRCLSETHGEREPCKKRLTARSISTPTCLGGSPVSEHPANVSPTAASLPQPEVILLDSDLDEPIDLRCVKMRSDAGEPPSTLQVKPEAPAVAAVVAPATASEKPPAEAQEEPVEPLSEFKPFFGNIIITDVTANCLTVTFKEYVTV

Target ORF information:

RefSeq Version	XM_021177758.1
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli chromobox 4 (Cbx4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021177758.1

ORF Insert Sequence:

ATGGAGCTGC CAGCTGTTGG CGAGCACGTC TTCGCGGTGG AGAGCATCGA AAAGAAGCGG 
ATCCGCAAGG GCAGAGTGGA GTATCTGGTG AAATGGAGAG GCTGGTCCCC CAAATATAAC 
ACGTGGGAAC CAGAGGAGAA CATCTTGGAT CCCCGTCTGC TGATCGCCTT CCAGAACAGG 
GAAAGGCAGG AGCAGCTGAT GGGATATCGC AAGAGAGGGC CCAAGCCCAA ACCGCTGGTG 
GTGCAGGTGC CCACCTTTGC CCGACGCTCC AATGTCCTGA CTGGGCTTCA AGACTCCTCC 
GCTGACAACC GTGCCAAGCT GGAGTTGGGC ACACAGGGCA AGGGCCAGGG GCACCAGTAT 
GAGCTCAACA GCAAGAAGCA CCATCAGTAC CAACCACATA GCAAGGAGAG GTCCGGTAAG 
CCGCCCCCGC CGGGGAAGAG CGGCAAGTAT TATTATCAGC TAAATAGCAA AAAGCACCAC 
CCCTACCAGC CAGACCCCAA AATGTACGAC CTGCAGTACC AGGGCGGCCA CAAGGAGGCA 
CCCAGCCCCA CCTGCCCAGA CCTGGGCACC AAGAGCCACC CTCCTGACAA GTGGGCCCAC 
GGAGCTGCAG CCAAAGGCTA CCTCGGAGCC GTGAAGCCCT TGGGGGGAGG GGCAGGAGCT 
CCAGGCAAGG GCTCGGAAAA GGGCCCCCCC AATGGTATGA CACCAGCCCC TAAGGAGGCA 
GTGACCGGTA ACGGTATTGG GGGCAAGATG AAAATTGTCA AGAACAAGAA CAAGAACGGG 
CGCATCGTGA TCGTTATGAG CAAGTACATG GAGAACGGCA TGCAAGCAGT GAAAATCAAG 
TCGGGCGAGG CCGGCGAGGG CGAGGCGCGC TCCCCCAGCC ATAAGAAACG TGCTGCCGAG 
GAGCGCCATC CCCAGAGCGA CAGGACTTTT AAAAAGGCAG CCGGTGCTTC TGAGGAGAAG 
AAAGCCGAAG TGCCCTGCAA ACGCAGGGAG GAGGAGGCTC TGGTGTCGGG GGACGCTCAG 
CCCCAGGACC TAGGGTCTAG AAAGCTCTCC CCGACCAAGG AGGCCTTTGG TGAGCAACCC 
CTACAGCTCA CCACCAAGCC AGACCTGCTG GCCTGGGACC CAGCCAGGAG CTCACACCCG 
CCCGCCCACC ACCACCATCA CCACCACCAT CACCACCACC ACCATACGGT GGGCCTGAAC 
CTCTCCCATG CGCGCAAGCG CTGCCTCTCC GAGACCCATG GCGAGCGTGA GCCCTGTAAG 
AAGCGGTTGA CCGCCCGCAG CATTAGCACG CCGACCTGCC TGGGGGGCAG CCCCGTCTCC 
GAGCACCCTG CCAACGTATC CCCCACCGCG GCCTCTCTGC CACAGCCCGA GGTCATCCTT 
TTGGACTCAG ACCTGGATGA ACCCATAGAC TTGCGCTGTG TCAAGATGCG CAGCGATGCC 
GGGGAACCGC CCAGCACCCT CCAGGTGAAG CCCGAGGCTC CGGCGGTGGC GGCAGTGGTG 
GCGCCGGCCA CCGCCTCGGA GAAACCTCCC GCTGAGGCCC AGGAGGAGCC AGTGGAGCCC 
CTGAGTGAGT TCAAGCCCTT CTTTGGGAAT ATAATTATCA CTGACGTCAC GGCAAACTGC 
CTTACCGTCA CTTTCAAGGA ATACGTGACC GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cbx4 ( XM_021177758.2 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021033417.1 Mus caroli chromobox 4 (Cbx4), mRNA.

Price & Availability↑

CloneID	OMv27678
Clone ID Related Accession (Same CDS sequence)	XM_021177758.1 , XM_021177758.2
Accession Version	XM_021177758.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-09
Organism	Mus caroli(Ryukyu mouse)
Product	E3 SUMO-protein ligase CBX4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034580.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 10, 2019 this sequence version replaced XM_021177758.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus caroli Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGC CAGCTGTTGG CGAGCACGTC TTCGCGGTGG AGAGCATCGA AAAGAAGCGG 
ATCCGCAAGG GCAGAGTGGA GTATCTGGTG AAATGGAGAG GCTGGTCCCC CAAATATAAC 
ACGTGGGAAC CAGAGGAGAA CATCTTGGAT CCCCGTCTGC TGATCGCCTT CCAGAACAGG 
GAAAGGCAGG AGCAGCTGAT GGGATATCGC AAGAGAGGGC CCAAGCCCAA ACCGCTGGTG 
GTGCAGGTGC CCACCTTTGC CCGACGCTCC AATGTCCTGA CTGGGCTTCA AGACTCCTCC 
GCTGACAACC GTGCCAAGCT GGAGTTGGGC ACACAGGGCA AGGGCCAGGG GCACCAGTAT 
GAGCTCAACA GCAAGAAGCA CCATCAGTAC CAACCACATA GCAAGGAGAG GTCCGGTAAG 
CCGCCCCCGC CGGGGAAGAG CGGCAAGTAT TATTATCAGC TAAATAGCAA AAAGCACCAC 
CCCTACCAGC CAGACCCCAA AATGTACGAC CTGCAGTACC AGGGCGGCCA CAAGGAGGCA 
CCCAGCCCCA CCTGCCCAGA CCTGGGCACC AAGAGCCACC CTCCTGACAA GTGGGCCCAC 
GGAGCTGCAG CCAAAGGCTA CCTCGGAGCC GTGAAGCCCT TGGGGGGAGG GGCAGGAGCT 
CCAGGCAAGG GCTCGGAAAA GGGCCCCCCC AATGGTATGA CACCAGCCCC TAAGGAGGCA 
GTGACCGGTA ACGGTATTGG GGGCAAGATG AAAATTGTCA AGAACAAGAA CAAGAACGGG 
CGCATCGTGA TCGTTATGAG CAAGTACATG GAGAACGGCA TGCAAGCAGT GAAAATCAAG 
TCGGGCGAGG CCGGCGAGGG CGAGGCGCGC TCCCCCAGCC ATAAGAAACG TGCTGCCGAG 
GAGCGCCATC CCCAGAGCGA CAGGACTTTT AAAAAGGCAG CCGGTGCTTC TGAGGAGAAG 
AAAGCCGAAG TGCCCTGCAA ACGCAGGGAG GAGGAGGCTC TGGTGTCGGG GGACGCTCAG 
CCCCAGGACC TAGGGTCTAG AAAGCTCTCC CCGACCAAGG AGGCCTTTGG TGAGCAACCC 
CTACAGCTCA CCACCAAGCC AGACCTGCTG GCCTGGGACC CAGCCAGGAG CTCACACCCG 
CCCGCCCACC ACCACCATCA CCACCACCAT CACCACCACC ACCATACGGT GGGCCTGAAC 
CTCTCCCATG CGCGCAAGCG CTGCCTCTCC GAGACCCATG GCGAGCGTGA GCCCTGTAAG 
AAGCGGTTGA CCGCCCGCAG CATTAGCACG CCGACCTGCC TGGGGGGCAG CCCCGTCTCC 
GAGCACCCTG CCAACGTATC CCCCACCGCG GCCTCTCTGC CACAGCCCGA GGTCATCCTT 
TTGGACTCAG ACCTGGATGA ACCCATAGAC TTGCGCTGTG TCAAGATGCG CAGCGATGCC 
GGGGAACCGC CCAGCACCCT CCAGGTGAAG CCCGAGGCTC CGGCGGTGGC GGCAGTGGTG 
GCGCCGGCCA CCGCCTCGGA GAAACCTCCC GCTGAGGCCC AGGAGGAGCC AGTGGAGCCC 
CTGAGTGAGT TCAAGCCCTT CTTTGGGAAT ATAATTATCA CTGACGTCAC GGCAAACTGC 
CTTACCGTCA CTTTCAAGGA ATACGTGACC GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021033417.1
CDS	223..1878
Translation	MELPAVGEHVFAVESIEKKRIRKGRVEYLVKWRGWSPKYNTWEPEENILDPRLLIAFQNRERQEQLMGYRKRGPKPKPLVVQVPTFARRSNVLTGLQDSSADNRAKLELGTQGKGQGHQYELNSKKHHQYQPHSKERSGKPPPPGKSGKYYYQLNSKKHHPYQPDPKMYDLQYQGGHKEAPSPTCPDLGTKSHPPDKWAHGAAAKGYLGAVKPLGGGAGAPGKGSEKGPPNGMTPAPKEAVTGNGIGGKMKIVKNKNKNGRIVIVMSKYMENGMQAVKIKSGEAGEGEARSPSHKKRAAEERHPQSDRTFKKAAGASEEKKAEVPCKRREEEALVSGDAQPQDLGSRKLSPTKEAFGEQPLQLTTKPDLLAWDPARSSHPPAHHHHHHHHHHHHHTVGLNLSHARKRCLSETHGEREPCKKRLTARSISTPTCLGGSPVSEHPANVSPTAASLPQPEVILLDSDLDEPIDLRCVKMRSDAGEPPSTLQVKPEAPAVAAVVAPATASEKPPAEAQEEPVEPLSEFKPFFGNIIITDVTANCLTVTFKEYVTV

Target ORF information:

RefSeq Version	XM_021177758.2
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli chromobox 4 (Cbx4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021177758.2

ORF Insert Sequence:

ATGGAGCTGC CAGCTGTTGG CGAGCACGTC TTCGCGGTGG AGAGCATCGA AAAGAAGCGG 
ATCCGCAAGG GCAGAGTGGA GTATCTGGTG AAATGGAGAG GCTGGTCCCC CAAATATAAC 
ACGTGGGAAC CAGAGGAGAA CATCTTGGAT CCCCGTCTGC TGATCGCCTT CCAGAACAGG 
GAAAGGCAGG AGCAGCTGAT GGGATATCGC AAGAGAGGGC CCAAGCCCAA ACCGCTGGTG 
GTGCAGGTGC CCACCTTTGC CCGACGCTCC AATGTCCTGA CTGGGCTTCA AGACTCCTCC 
GCTGACAACC GTGCCAAGCT GGAGTTGGGC ACACAGGGCA AGGGCCAGGG GCACCAGTAT 
GAGCTCAACA GCAAGAAGCA CCATCAGTAC CAACCACATA GCAAGGAGAG GTCCGGTAAG 
CCGCCCCCGC CGGGGAAGAG CGGCAAGTAT TATTATCAGC TAAATAGCAA AAAGCACCAC 
CCCTACCAGC CAGACCCCAA AATGTACGAC CTGCAGTACC AGGGCGGCCA CAAGGAGGCA 
CCCAGCCCCA CCTGCCCAGA CCTGGGCACC AAGAGCCACC CTCCTGACAA GTGGGCCCAC 
GGAGCTGCAG CCAAAGGCTA CCTCGGAGCC GTGAAGCCCT TGGGGGGAGG GGCAGGAGCT 
CCAGGCAAGG GCTCGGAAAA GGGCCCCCCC AATGGTATGA CACCAGCCCC TAAGGAGGCA 
GTGACCGGTA ACGGTATTGG GGGCAAGATG AAAATTGTCA AGAACAAGAA CAAGAACGGG 
CGCATCGTGA TCGTTATGAG CAAGTACATG GAGAACGGCA TGCAAGCAGT GAAAATCAAG 
TCGGGCGAGG CCGGCGAGGG CGAGGCGCGC TCCCCCAGCC ATAAGAAACG TGCTGCCGAG 
GAGCGCCATC CCCAGAGCGA CAGGACTTTT AAAAAGGCAG CCGGTGCTTC TGAGGAGAAG 
AAAGCCGAAG TGCCCTGCAA ACGCAGGGAG GAGGAGGCTC TGGTGTCGGG GGACGCTCAG 
CCCCAGGACC TAGGGTCTAG AAAGCTCTCC CCGACCAAGG AGGCCTTTGG TGAGCAACCC 
CTACAGCTCA CCACCAAGCC AGACCTGCTG GCCTGGGACC CAGCCAGGAG CTCACACCCG 
CCCGCCCACC ACCACCATCA CCACCACCAT CACCACCACC ACCATACGGT GGGCCTGAAC 
CTCTCCCATG CGCGCAAGCG CTGCCTCTCC GAGACCCATG GCGAGCGTGA GCCCTGTAAG 
AAGCGGTTGA CCGCCCGCAG CATTAGCACG CCGACCTGCC TGGGGGGCAG CCCCGTCTCC 
GAGCACCCTG CCAACGTATC CCCCACCGCG GCCTCTCTGC CACAGCCCGA GGTCATCCTT 
TTGGACTCAG ACCTGGATGA ACCCATAGAC TTGCGCTGTG TCAAGATGCG CAGCGATGCC 
GGGGAACCGC CCAGCACCCT CCAGGTGAAG CCCGAGGCTC CGGCGGTGGC GGCAGTGGTG 
GCGCCGGCCA CCGCCTCGGA GAAACCTCCC GCTGAGGCCC AGGAGGAGCC AGTGGAGCCC 
CTGAGTGAGT TCAAGCCCTT CTTTGGGAAT ATAATTATCA CTGACGTCAC GGCAAACTGC 
CTTACCGTCA CTTTCAAGGA ATACGTGACC GTGTAG