Dgkk cDNA ORF clone, Mus caroli(Ryukyu mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dgkk
Entrez Gene ID	110287220
Full Name	diacylglycerol kinase kappa
Gene Type	protein-coding
Organism	Mus caroli(Ryukyu mouse)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021153875.1	XP_021009534.1	diacylglycerol kinase kappa isoform X1
XM_021153876.1	XP_021009535.1	diacylglycerol kinase kappa isoform X2

Pathways from BioSystems
Gene Ontology

The following Dgkk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dgkk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMv10548	XM_021153876.1 Latest version!	Mus caroli diacylglycerol kinase kappa (Dgkk), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OMv10547	XM_021153875.1 Latest version!	Mus caroli diacylglycerol kinase kappa (Dgkk), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dgkk ( XM_021153876.1 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021009535.1 Mus caroli diacylglycerol kinase kappa (Dgkk), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMv10548
Clone ID Related Accession (Same CDS sequence)	XM_021153876.1
Accession Version	XM_021153876.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-09
Organism	Mus caroli(Ryukyu mouse)
Product	diacylglycerol kinase kappa isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034589.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus caroli Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGGG CCCCAGAGTC CTGGCCAGAG CCAACTCCAG AGTCCTGTCT AAAGATGAGT 
CCAGCACTAC ATCTGTTGCA GGTAGTTCCT GGAGAGAAAA GTGGAACGAC ATCTCCAGTG 
CTATTGCCAT TGCCGTCGCC CAAACCGCCA ATAACGTGGT CCAGAATAAA GAAAATACTG 
AAGGAAGGAC CCCTGCTGAA GAACTGTAAC TCCTTCAGAA GATGGAAGCT TAGATACTGT 
CTGGTTCAAG GGCAGAAGCT CCACTTTGCA CACCATCCTT CGTTTGCACA CTTTGAAACA 
ATTGATCTGT CTCAAGTTGT CTTGGCTGAA AGTAGCTGCA GAAATCTTTG CCACGGTTTT 
TGTGTTATCA CACCACATCG AAAAGTATCT CTAGTTGCAC CCACTCGGCA AGACATGGAA 
GAATGGATTA ACATCATAAA AACCGTCCAA CAAGGAGAAA TCTGTCAGAT CCCTGCAGCC 
GAAAACAACC CCTTTCTTGT CGGAATGCAC TACTGGTATT CCAGTAATAA TCCCCGGAGT 
CAGTTCTGCA ATGTTTGTCG AGAGAACATT CCTGCTTTAT CTCGAGATGC CGTCACCTGT 
GAAGTCTGTC AGGTGAAATC TCATAAATTC TGTGCTTTGA GAGCAAACAA AGACTGTAAA 
TGGAATACTT TATCCGTGAC TGATGACCTT CTTCTACCCG CTGATGAAAT ACAAACAATG 
CCGCATCAGT GGGTAGAAGG AAATATACCC GCTGGCTCTC AATGTGTCGT ATGTCACAAG 
AGCTGCGGTA GTCATCACAG ACTTCAGGAT TTCCGCTGCC TCTGGTGCGG CTCTACGGTG 
CACGGGGCCT GCCAGAAGCG GTTTTCCAAG GAATGTTCCT TCGGAAGTCG TCGCTCGTCC 
ATCGTGCCAC CAACGGCACT GAGCGACCCT AGGGGCGATG GCCAGCTAGT AGTATCGCCT 
GACTTCTGGA ATCTCGACTG GCCACTGACT TGTTCCTGTC CCTTGCTCAT CTTCATCAAC 
TCTAAAAGTG GAGATCATCA AGGGATCATC TTTCTCCGGA AATTCAAACA ATACCTTAAT 
CCGTCTCAAG TATTCGACCT GGCGAAGGGT GGACCTGAAG CAGGCATTGC TATGTTCAAG 
AACTTTGCCC GTTTTCGTGT TCTGGTTTGT GGTGGAGATG GCAGTGTGAG CTGGGTCTTG 
TCTACAATTG ATGCCTATGG ATTGCATGAT AGGTGTCAGC TGGCGATCAT CCCACTTGGA 
ACTGGTAATG ATCTCGCTCG TGTCCTTGGC TGGGGAGCAT TCTGGAGTAA AGGCACATCA 
CCACTGGATA TTCTCAGCAG AGTGGAGCAG GCTCACGTGA GGATTTTAGA CAGATGGAGT 
GTGATGATCC GTGAGACCCC CAGACAAGCC CCACTGCTGA AAGGACAAGT GGCAATGGAT 
ATACCAAGAT TTGAGGCTGC TGCCATCAAG AACGTAGAGT CTGCAACCAC TGAGTTGAAC 
AAAATTCTGA AGGCCAAATA TCCCACCGAG ATGGTCATCG CCACAAGATT CCTGTGCTCA 
GCAGTGGAAG ATTTTGTGGA AGATATTGTA AAGGCCTGGC ACCAAATAAA ACAGAACAGC 
ACAGCAGTGG AGTCTGTGAT TTTGAAAAGT GACTTAATGT ATGATAAACT CAGTGTCCTT 
ATTGATCTCC TGGCTGAGGA TGCAGTAGCT GCCTCCGCTG AGAGGACAGC CACAGCATAT 
GGAAGCAGAA GCAAAGCAGA TGGAAAACCC TTCGTGCCTA AACTAGACCA CATAGCAAGG 
GCCAAACTGG AGTTGGCTGA AAGGGCTCAG AAACTCCAGC AATCCTTGAA ACTCATCATA 
TTCCAGGTCG AACAAGTTCT GGATGAAGAA AGCAGACAGA GCTTATCCGT TAAGAACTTT 
ACTTCATCTT TGTTCCTGGG AGACGGAGAC GACGACTCAG ATGACTATGA TCAGAGCCCT 
AGACACCGTT CTCGTTGCGA CATCTTATGT TCTATACCTT CTTTGAGAAG TGAAGACCTA 
GATAACCTTG ACTTAGAACA CTTACACATT GCACCTGAAA CGATACGCTT CAAAGAAAAG 
TGTGTCATGA ACAACTACTT TGGAATTGGA CTAGATGCAA AAATCTCTCT GGAGTTTAAT 
TCCAGAAGAG AAGAACACCC AGAACAATAC AATAGCCGCC TCAAGAACAA AATATGGTAT 
GGTCTTCTGG GAAGCAAGGA AGTACTGCAA CGCTCTTACA GGAAATTGGA AGAGCGAATA 
CACCTCGAGT GTGATGGAGA ACCTGTCTCC TTGCCAAACC TTCAAGGCAT CGTAGTGCTC 
AACATTACGA GCTACGCTGG AGGCGTCAAC TTTTGGGGAA GGAACAGAGC AACCACAGAA 
TATGATGTCC CTGCAATCAA TGATGGGAAG CTAGAGGTTG TGGCAATCTT TGGTTCTGTG 
CAGATGGCCA TGTCTCGTAT TGTCAACCTG CAACAACATC GAATTGCGCA GTGCCATGAG 
GTGGTAATAA CTATTGATGG TGAAGACGGT GTCCCTGTGC AAGTGGATGG GGAAGCCTGG 
ATTCAGAAGC CAGGCCTTAT CAAGATTAAA TACAAGAATG TTGCCCAGAT GTTGATGAGA 
GATAGGGACT TTGAGAACTC AATGAAAAAC TGGGAATCTA AGCATACTGA AATCCAAGCT 
GTCCAACCAC CCCACTTGGA TTTCCAGGAA TCTCAAGACA GCCTCTCTGA TGGAGAGTAT 
GCCCAGATGC AGCACTTGGC TCGGCTTGCA GAAAACCTCA TTAGCAGACT TACTGACCTA 
AGCAAGGTCC ACCAGCACGT GTCTGTCCTC ATGGATTCTG TGAATGCCAG TGCTAGCATA 
CTGAATGATG TGTTCTATAG CCAAGACAGT GATACCGAAG CAGGTGCAGC TTCCTGCATT 
CCCATTGAGA CTCTAAGCAG AACTGATGCA GTAGATGTTA CATTTAGTCT TAAAGGGCTC 
TACGATGACA CCAAAGCTTT CCTGGATGAA AACTTGTTGA GAGATGCTGA GGATCGGGAC 
ATGCTACAAA CTGCCTTGGA TGCCATGAAT ACAGAGTTAA GAAGGATACT TGCAATTGGC 
TGGTTGAGTC AAATCTTCTT TCCAGAGGAA CAGGCTTCTG ACACTCGCAG TTTAAGTCGT 
AGGTTTAGAA TGAAATTCCC CAAGCTGGGG AAGAAGAAAC AACGAGAAGA GGGAGAAAAG 
CCTAAATCAA GCCAAAGATT CCCTGGTTTT CTTGGCAAGT TCTGGCGTCG CAGAAATCGT 
AGCAATCAAG CAAAAGCTGA TGACCCTCCA ACACCTTCAA GTTCTCAACT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021009535.1
CDS	155..3508
Translation	MERAPESWPEPTPESCLKMSPALHLLQVVPGEKSGTTSPVLLPLPSPKPPITWSRIKKILKEGPLLKNCNSFRRWKLRYCLVQGQKLHFAHHPSFAHFETIDLSQVVLAESSCRNLCHGFCVITPHRKVSLVAPTRQDMEEWINIIKTVQQGEICQIPAAENNPFLVGMHYWYSSNNPRSQFCNVCRENIPALSRDAVTCEVCQVKSHKFCALRANKDCKWNTLSVTDDLLLPADEIQTMPHQWVEGNIPAGSQCVVCHKSCGSHHRLQDFRCLWCGSTVHGACQKRFSKECSFGSRRSSIVPPTALSDPRGDGQLVVSPDFWNLDWPLTCSCPLLIFINSKSGDHQGIIFLRKFKQYLNPSQVFDLAKGGPEAGIAMFKNFARFRVLVCGGDGSVSWVLSTIDAYGLHDRCQLAIIPLGTGNDLARVLGWGAFWSKGTSPLDILSRVEQAHVRILDRWSVMIRETPRQAPLLKGQVAMDIPRFEAAAIKNVESATTELNKILKAKYPTEMVIATRFLCSAVEDFVEDIVKAWHQIKQNSTAVESVILKSDLMYDKLSVLIDLLAEDAVAASAERTATAYGSRSKADGKPFVPKLDHIARAKLELAERAQKLQQSLKLIIFQVEQVLDEESRQSLSVKNFTSSLFLGDGDDDSDDYDQSPRHRSRCDILCSIPSLRSEDLDNLDLEHLHIAPETIRFKEKCVMNNYFGIGLDAKISLEFNSRREEHPEQYNSRLKNKIWYGLLGSKEVLQRSYRKLEERIHLECDGEPVSLPNLQGIVVLNITSYAGGVNFWGRNRATTEYDVPAINDGKLEVVAIFGSVQMAMSRIVNLQQHRIAQCHEVVITIDGEDGVPVQVDGEAWIQKPGLIKIKYKNVAQMLMRDRDFENSMKNWESKHTEIQAVQPPHLDFQESQDSLSDGEYAQMQHLARLAENLISRLTDLSKVHQHVSVLMDSVNASASILNDVFYSQDSDTEAGAASCIPIETLSRTDAVDVTFSLKGLYDDTKAFLDENLLRDAEDRDMLQTALDAMNTELRRILAIGWLSQIFFPEEQASDTRSLSRRFRMKFPKLGKKKQREEGEKPKSSQRFPGFLGKFWRRRNRSNQAKADDPPTPSSSQL

Target ORF information:

RefSeq Version	XM_021153876.1
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli diacylglycerol kinase kappa (Dgkk), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021153876.1

ORF Insert Sequence:

ATGGAGAGGG CCCCAGAGTC CTGGCCAGAG CCAACTCCAG AGTCCTGTCT AAAGATGAGT 
CCAGCACTAC ATCTGTTGCA GGTAGTTCCT GGAGAGAAAA GTGGAACGAC ATCTCCAGTG 
CTATTGCCAT TGCCGTCGCC CAAACCGCCA ATAACGTGGT CCAGAATAAA GAAAATACTG 
AAGGAAGGAC CCCTGCTGAA GAACTGTAAC TCCTTCAGAA GATGGAAGCT TAGATACTGT 
CTGGTTCAAG GGCAGAAGCT CCACTTTGCA CACCATCCTT CGTTTGCACA CTTTGAAACA 
ATTGATCTGT CTCAAGTTGT CTTGGCTGAA AGTAGCTGCA GAAATCTTTG CCACGGTTTT 
TGTGTTATCA CACCACATCG AAAAGTATCT CTAGTTGCAC CCACTCGGCA AGACATGGAA 
GAATGGATTA ACATCATAAA AACCGTCCAA CAAGGAGAAA TCTGTCAGAT CCCTGCAGCC 
GAAAACAACC CCTTTCTTGT CGGAATGCAC TACTGGTATT CCAGTAATAA TCCCCGGAGT 
CAGTTCTGCA ATGTTTGTCG AGAGAACATT CCTGCTTTAT CTCGAGATGC CGTCACCTGT 
GAAGTCTGTC AGGTGAAATC TCATAAATTC TGTGCTTTGA GAGCAAACAA AGACTGTAAA 
TGGAATACTT TATCCGTGAC TGATGACCTT CTTCTACCCG CTGATGAAAT ACAAACAATG 
CCGCATCAGT GGGTAGAAGG AAATATACCC GCTGGCTCTC AATGTGTCGT ATGTCACAAG 
AGCTGCGGTA GTCATCACAG ACTTCAGGAT TTCCGCTGCC TCTGGTGCGG CTCTACGGTG 
CACGGGGCCT GCCAGAAGCG GTTTTCCAAG GAATGTTCCT TCGGAAGTCG TCGCTCGTCC 
ATCGTGCCAC CAACGGCACT GAGCGACCCT AGGGGCGATG GCCAGCTAGT AGTATCGCCT 
GACTTCTGGA ATCTCGACTG GCCACTGACT TGTTCCTGTC CCTTGCTCAT CTTCATCAAC 
TCTAAAAGTG GAGATCATCA AGGGATCATC TTTCTCCGGA AATTCAAACA ATACCTTAAT 
CCGTCTCAAG TATTCGACCT GGCGAAGGGT GGACCTGAAG CAGGCATTGC TATGTTCAAG 
AACTTTGCCC GTTTTCGTGT TCTGGTTTGT GGTGGAGATG GCAGTGTGAG CTGGGTCTTG 
TCTACAATTG ATGCCTATGG ATTGCATGAT AGGTGTCAGC TGGCGATCAT CCCACTTGGA 
ACTGGTAATG ATCTCGCTCG TGTCCTTGGC TGGGGAGCAT TCTGGAGTAA AGGCACATCA 
CCACTGGATA TTCTCAGCAG AGTGGAGCAG GCTCACGTGA GGATTTTAGA CAGATGGAGT 
GTGATGATCC GTGAGACCCC CAGACAAGCC CCACTGCTGA AAGGACAAGT GGCAATGGAT 
ATACCAAGAT TTGAGGCTGC TGCCATCAAG AACGTAGAGT CTGCAACCAC TGAGTTGAAC 
AAAATTCTGA AGGCCAAATA TCCCACCGAG ATGGTCATCG CCACAAGATT CCTGTGCTCA 
GCAGTGGAAG ATTTTGTGGA AGATATTGTA AAGGCCTGGC ACCAAATAAA ACAGAACAGC 
ACAGCAGTGG AGTCTGTGAT TTTGAAAAGT GACTTAATGT ATGATAAACT CAGTGTCCTT 
ATTGATCTCC TGGCTGAGGA TGCAGTAGCT GCCTCCGCTG AGAGGACAGC CACAGCATAT 
GGAAGCAGAA GCAAAGCAGA TGGAAAACCC TTCGTGCCTA AACTAGACCA CATAGCAAGG 
GCCAAACTGG AGTTGGCTGA AAGGGCTCAG AAACTCCAGC AATCCTTGAA ACTCATCATA 
TTCCAGGTCG AACAAGTTCT GGATGAAGAA AGCAGACAGA GCTTATCCGT TAAGAACTTT 
ACTTCATCTT TGTTCCTGGG AGACGGAGAC GACGACTCAG ATGACTATGA TCAGAGCCCT 
AGACACCGTT CTCGTTGCGA CATCTTATGT TCTATACCTT CTTTGAGAAG TGAAGACCTA 
GATAACCTTG ACTTAGAACA CTTACACATT GCACCTGAAA CGATACGCTT CAAAGAAAAG 
TGTGTCATGA ACAACTACTT TGGAATTGGA CTAGATGCAA AAATCTCTCT GGAGTTTAAT 
TCCAGAAGAG AAGAACACCC AGAACAATAC AATAGCCGCC TCAAGAACAA AATATGGTAT 
GGTCTTCTGG GAAGCAAGGA AGTACTGCAA CGCTCTTACA GGAAATTGGA AGAGCGAATA 
CACCTCGAGT GTGATGGAGA ACCTGTCTCC TTGCCAAACC TTCAAGGCAT CGTAGTGCTC 
AACATTACGA GCTACGCTGG AGGCGTCAAC TTTTGGGGAA GGAACAGAGC AACCACAGAA 
TATGATGTCC CTGCAATCAA TGATGGGAAG CTAGAGGTTG TGGCAATCTT TGGTTCTGTG 
CAGATGGCCA TGTCTCGTAT TGTCAACCTG CAACAACATC GAATTGCGCA GTGCCATGAG 
GTGGTAATAA CTATTGATGG TGAAGACGGT GTCCCTGTGC AAGTGGATGG GGAAGCCTGG 
ATTCAGAAGC CAGGCCTTAT CAAGATTAAA TACAAGAATG TTGCCCAGAT GTTGATGAGA 
GATAGGGACT TTGAGAACTC AATGAAAAAC TGGGAATCTA AGCATACTGA AATCCAAGCT 
GTCCAACCAC CCCACTTGGA TTTCCAGGAA TCTCAAGACA GCCTCTCTGA TGGAGAGTAT 
GCCCAGATGC AGCACTTGGC TCGGCTTGCA GAAAACCTCA TTAGCAGACT TACTGACCTA 
AGCAAGGTCC ACCAGCACGT GTCTGTCCTC ATGGATTCTG TGAATGCCAG TGCTAGCATA 
CTGAATGATG TGTTCTATAG CCAAGACAGT GATACCGAAG CAGGTGCAGC TTCCTGCATT 
CCCATTGAGA CTCTAAGCAG AACTGATGCA GTAGATGTTA CATTTAGTCT TAAAGGGCTC 
TACGATGACA CCAAAGCTTT CCTGGATGAA AACTTGTTGA GAGATGCTGA GGATCGGGAC 
ATGCTACAAA CTGCCTTGGA TGCCATGAAT ACAGAGTTAA GAAGGATACT TGCAATTGGC 
TGGTTGAGTC AAATCTTCTT TCCAGAGGAA CAGGCTTCTG ACACTCGCAG TTTAAGTCGT 
AGGTTTAGAA TGAAATTCCC CAAGCTGGGG AAGAAGAAAC AACGAGAAGA GGGAGAAAAG 
CCTAAATCAA GCCAAAGATT CCCTGGTTTT CTTGGCAAGT TCTGGCGTCG CAGAAATCGT 
AGCAATCAAG CAAAAGCTGA TGACCCTCCA ACACCTTCAA GTTCTCAACT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dgkk ( XM_021153875.1 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021009534.1 Mus caroli diacylglycerol kinase kappa (Dgkk), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMv10547
Clone ID Related Accession (Same CDS sequence)	XM_021153875.1
Accession Version	XM_021153875.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-09
Organism	Mus caroli(Ryukyu mouse)
Product	diacylglycerol kinase kappa isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034589.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus caroli Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGGTG TAGATTCGGT GGCCCAGGGC ACTGCCCAGC CTCAGGATGA AGAGCAGCCT 
GCTGAGTCCC CAGAGCCGCC GCCGCCACCT CCTGCTCCGC CGCCAGCCCC ACCGCCTCTG 
GCCCCACCGC CTCCGGCCCC ACCGCCTCCG GCCCCACCGC CGCCGGCCCC ACCACCGCCT 
GCTCCTCCAC CATTCCCTGA GCCTTCTCCA GAACCAGTAC CAGAGCCTGC TCTGGGACCC 
TGTCCAGAGG CAACTCCAGA ACCAGCCACA GAAACAGACG CAAAATCAAC CCTAGAACCG 
GCCCCGAAGC CGGACTCAGA ACCGGACTCG GACCCGGACC CCGAACCAGA CTCAGAGCCG 
GCCCCAGAGC CGGCCCCCGA ACCTGACTCA GAGCCGGCCC CAGAGCCGGT CCCCGAACCA 
GACTCAGAGC CGACCCCAGA GCCGGCCCCC GAACCTGACT CAGAGCCGGC CCCAGAGCCG 
GACCCCGAAC TAGACTCAGA GCCGGCCCCA GAGCCGGCCC CCGAACCAGA CTCAGAACCG 
GCCCCAGAAC CAGTCTCAGA GCCGGCCCCA CAACTTGTCC CTGAGCTGGC CCCGGAGCCG 
GCCCCAGAAT CTATGAGTCC AGCACTACAT CTGTTGCAGG TAGTTCCTGG AGAGAAAAGT 
GGAACGACAT CTCCAGTGCT ATTGCCATTG CCGTCGCCCA AACCGCCAAT AACGTGGTCC 
AGAATAAAGA AAATACTGAA GGAAGGACCC CTGCTGAAGA ACTGTAACTC CTTCAGAAGA 
TGGAAGCTTA GATACTGTCT GGTTCAAGGG CAGAAGCTCC ACTTTGCACA CCATCCTTCG 
TTTGCACACT TTGAAACAAT TGATCTGTCT CAAGTTGTCT TGGCTGAAAG TAGCTGCAGA 
AATCTTTGCC ACGGTTTTTG TGTTATCACA CCACATCGAA AAGTATCTCT AGTTGCACCC 
ACTCGGCAAG ACATGGAAGA ATGGATTAAC ATCATAAAAA CCGTCCAACA AGGAGAAATC 
TGTCAGATCC CTGCAGCCGA AAACAACCCC TTTCTTGTCG GAATGCACTA CTGGTATTCC 
AGTAATAATC CCCGGAGTCA GTTCTGCAAT GTTTGTCGAG AGAACATTCC TGCTTTATCT 
CGAGATGCCG TCACCTGTGA AGTCTGTCAG GTGAAATCTC ATAAATTCTG TGCTTTGAGA 
GCAAACAAAG ACTGTAAATG GAATACTTTA TCCGTGACTG ATGACCTTCT TCTACCCGCT 
GATGAAATAC AAACAATGCC GCATCAGTGG GTAGAAGGAA ATATACCCGC TGGCTCTCAA 
TGTGTCGTAT GTCACAAGAG CTGCGGTAGT CATCACAGAC TTCAGGATTT CCGCTGCCTC 
TGGTGCGGCT CTACGGTGCA CGGGGCCTGC CAGAAGCGGT TTTCCAAGGA ATGTTCCTTC 
GGAAGTCGTC GCTCGTCCAT CGTGCCACCA ACGGCACTGA GCGACCCTAG GGGCGATGGC 
CAGCTAGTAG TATCGCCTGA CTTCTGGAAT CTCGACTGGC CACTGACTTG TTCCTGTCCC 
TTGCTCATCT TCATCAACTC TAAAAGTGGA GATCATCAAG GGATCATCTT TCTCCGGAAA 
TTCAAACAAT ACCTTAATCC GTCTCAAGTA TTCGACCTGG CGAAGGGTGG ACCTGAAGCA 
GGCATTGCTA TGTTCAAGAA CTTTGCCCGT TTTCGTGTTC TGGTTTGTGG TGGAGATGGC 
AGTGTGAGCT GGGTCTTGTC TACAATTGAT GCCTATGGAT TGCATGATAG GTGTCAGCTG 
GCGATCATCC CACTTGGAAC TGGTAATGAT CTCGCTCGTG TCCTTGGCTG GGGAGCATTC 
TGGAGTAAAG GCACATCACC ACTGGATATT CTCAGCAGAG TGGAGCAGGC TCACGTGAGG 
ATTTTAGACA GATGGAGTGT GATGATCCGT GAGACCCCCA GACAAGCCCC ACTGCTGAAA 
GGACAAGTGG CAATGGATAT ACCAAGATTT GAGGCTGCTG CCATCAAGAA CGTAGAGTCT 
GCAACCACTG AGTTGAACAA AATTCTGAAG GCCAAATATC CCACCGAGAT GGTCATCGCC 
ACAAGATTCC TGTGCTCAGC AGTGGAAGAT TTTGTGGAAG ATATTGTAAA GGCCTGGCAC 
CAAATAAAAC AGAACAGCAC AGCAGTGGAG TCTGTGATTT TGAAAAGTGA CTTAATGTAT 
GATAAACTCA GTGTCCTTAT TGATCTCCTG GCTGAGGATG CAGTAGCTGC CTCCGCTGAG 
AGGACAGCCA CAGCATATGG AAGCAGAAGC AAAGCAGATG GAAAACCCTT CGTGCCTAAA 
CTAGACCACA TAGCAAGGGC CAAACTGGAG TTGGCTGAAA GGGCTCAGAA ACTCCAGCAA 
TCCTTGAAAC TCATCATATT CCAGGTCGAA CAAGTTCTGG ATGAAGAAAG CAGACAGAGC 
TTATCCGTTA AGAACTTTAC TTCATCTTTG TTCCTGGGAG ACGGAGACGA CGACTCAGAT 
GACTATGATC AGAGCCCTAG ACACCGTTCT CGTTGCGACA TCTTATGTTC TATACCTTCT 
TTGAGAAGTG AAGACCTAGA TAACCTTGAC TTAGAACACT TACACATTGC ACCTGAAACG 
ATACGCTTCA AAGAAAAGTG TGTCATGAAC AACTACTTTG GAATTGGACT AGATGCAAAA 
ATCTCTCTGG AGTTTAATTC CAGAAGAGAA GAACACCCAG AACAATACAA TAGCCGCCTC 
AAGAACAAAA TATGGTATGG TCTTCTGGGA AGCAAGGAAG TACTGCAACG CTCTTACAGG 
AAATTGGAAG AGCGAATACA CCTCGAGTGT GATGGAGAAC CTGTCTCCTT GCCAAACCTT 
CAAGGCATCG TAGTGCTCAA CATTACGAGC TACGCTGGAG GCGTCAACTT TTGGGGAAGG 
AACAGAGCAA CCACAGAATA TGATGTCCCT GCAATCAATG ATGGGAAGCT AGAGGTTGTG 
GCAATCTTTG GTTCTGTGCA GATGGCCATG TCTCGTATTG TCAACCTGCA ACAACATCGA 
ATTGCGCAGT GCCATGAGGT GGTAATAACT ATTGATGGTG AAGACGGTGT CCCTGTGCAA 
GTGGATGGGG AAGCCTGGAT TCAGAAGCCA GGCCTTATCA AGATTAAATA CAAGAATGTT 
GCCCAGATGT TGATGAGAGA TAGGGACTTT GAGAACTCAA TGAAAAACTG GGAATCTAAG 
CATACTGAAA TCCAAGCTGT CCAACCACCC CACTTGGATT TCCAGGAATC TCAAGACAGC 
CTCTCTGATG GAGAGTATGC CCAGATGCAG CACTTGGCTC GGCTTGCAGA AAACCTCATT 
AGCAGACTTA CTGACCTAAG CAAGGTCCAC CAGCACGTGT CTGTCCTCAT GGATTCTGTG 
AATGCCAGTG CTAGCATACT GAATGATGTG TTCTATAGCC AAGACAGTGA TACCGAAGCA 
GGTGCAGCTT CCTGCATTCC CATTGAGACT CTAAGCAGAA CTGATGCAGT AGATGTTACA 
TTTAGTCTTA AAGGGCTCTA CGATGACACC AAAGCTTTCC TGGATGAAAA CTTGTTGAGA 
GATGCTGAGG ATCGGGACAT GCTACAAACT GCCTTGGATG CCATGAATAC AGAGTTAAGA 
AGGATACTTG CAATTGGCTG GTTGAGTCAA ATCTTCTTTC CAGAGGAACA GGCTTCTGAC 
ACTCGCAGTT TAAGTCGTAG GTTTAGAATG AAATTCCCCA AGCTGGGGAA GAAGAAACAA 
CGAGAAGAGG GAGAAAAGCC TAAATCAAGC CAAAGATTCC CTGGTTTTCT TGGCAAGTTC 
TGGCGTCGCA GAAATCGTAG CAATCAAGCA AAAGCTGATG ACCCTCCAAC ACCTTCAAGT 
TCTCAACTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021009534.1
CDS	1..3912
Translation	MDGVDSVAQGTAQPQDEEQPAESPEPPPPPPAPPPAPPPLAPPPPAPPPPAPPPPAPPPPAPPPFPEPSPEPVPEPALGPCPEATPEPATETDAKSTLEPAPKPDSEPDSDPDPEPDSEPAPEPAPEPDSEPAPEPVPEPDSEPTPEPAPEPDSEPAPEPDPELDSEPAPEPAPEPDSEPAPEPVSEPAPQLVPELAPEPAPESMSPALHLLQVVPGEKSGTTSPVLLPLPSPKPPITWSRIKKILKEGPLLKNCNSFRRWKLRYCLVQGQKLHFAHHPSFAHFETIDLSQVVLAESSCRNLCHGFCVITPHRKVSLVAPTRQDMEEWINIIKTVQQGEICQIPAAENNPFLVGMHYWYSSNNPRSQFCNVCRENIPALSRDAVTCEVCQVKSHKFCALRANKDCKWNTLSVTDDLLLPADEIQTMPHQWVEGNIPAGSQCVVCHKSCGSHHRLQDFRCLWCGSTVHGACQKRFSKECSFGSRRSSIVPPTALSDPRGDGQLVVSPDFWNLDWPLTCSCPLLIFINSKSGDHQGIIFLRKFKQYLNPSQVFDLAKGGPEAGIAMFKNFARFRVLVCGGDGSVSWVLSTIDAYGLHDRCQLAIIPLGTGNDLARVLGWGAFWSKGTSPLDILSRVEQAHVRILDRWSVMIRETPRQAPLLKGQVAMDIPRFEAAAIKNVESATTELNKILKAKYPTEMVIATRFLCSAVEDFVEDIVKAWHQIKQNSTAVESVILKSDLMYDKLSVLIDLLAEDAVAASAERTATAYGSRSKADGKPFVPKLDHIARAKLELAERAQKLQQSLKLIIFQVEQVLDEESRQSLSVKNFTSSLFLGDGDDDSDDYDQSPRHRSRCDILCSIPSLRSEDLDNLDLEHLHIAPETIRFKEKCVMNNYFGIGLDAKISLEFNSRREEHPEQYNSRLKNKIWYGLLGSKEVLQRSYRKLEERIHLECDGEPVSLPNLQGIVVLNITSYAGGVNFWGRNRATTEYDVPAINDGKLEVVAIFGSVQMAMSRIVNLQQHRIAQCHEVVITIDGEDGVPVQVDGEAWIQKPGLIKIKYKNVAQMLMRDRDFENSMKNWESKHTEIQAVQPPHLDFQESQDSLSDGEYAQMQHLARLAENLISRLTDLSKVHQHVSVLMDSVNASASILNDVFYSQDSDTEAGAASCIPIETLSRTDAVDVTFSLKGLYDDTKAFLDENLLRDAEDRDMLQTALDAMNTELRRILAIGWLSQIFFPEEQASDTRSLSRRFRMKFPKLGKKKQREEGEKPKSSQRFPGFLGKFWRRRNRSNQAKADDPPTPSSSQL

Target ORF information:

RefSeq Version	XM_021153875.1
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli diacylglycerol kinase kappa (Dgkk), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021153875.1

ORF Insert Sequence:

ATGGACGGTG TAGATTCGGT GGCCCAGGGC ACTGCCCAGC CTCAGGATGA AGAGCAGCCT 
GCTGAGTCCC CAGAGCCGCC GCCGCCACCT CCTGCTCCGC CGCCAGCCCC ACCGCCTCTG 
GCCCCACCGC CTCCGGCCCC ACCGCCTCCG GCCCCACCGC CGCCGGCCCC ACCACCGCCT 
GCTCCTCCAC CATTCCCTGA GCCTTCTCCA GAACCAGTAC CAGAGCCTGC TCTGGGACCC 
TGTCCAGAGG CAACTCCAGA ACCAGCCACA GAAACAGACG CAAAATCAAC CCTAGAACCG 
GCCCCGAAGC CGGACTCAGA ACCGGACTCG GACCCGGACC CCGAACCAGA CTCAGAGCCG 
GCCCCAGAGC CGGCCCCCGA ACCTGACTCA GAGCCGGCCC CAGAGCCGGT CCCCGAACCA 
GACTCAGAGC CGACCCCAGA GCCGGCCCCC GAACCTGACT CAGAGCCGGC CCCAGAGCCG 
GACCCCGAAC TAGACTCAGA GCCGGCCCCA GAGCCGGCCC CCGAACCAGA CTCAGAACCG 
GCCCCAGAAC CAGTCTCAGA GCCGGCCCCA CAACTTGTCC CTGAGCTGGC CCCGGAGCCG 
GCCCCAGAAT CTATGAGTCC AGCACTACAT CTGTTGCAGG TAGTTCCTGG AGAGAAAAGT 
GGAACGACAT CTCCAGTGCT ATTGCCATTG CCGTCGCCCA AACCGCCAAT AACGTGGTCC 
AGAATAAAGA AAATACTGAA GGAAGGACCC CTGCTGAAGA ACTGTAACTC CTTCAGAAGA 
TGGAAGCTTA GATACTGTCT GGTTCAAGGG CAGAAGCTCC ACTTTGCACA CCATCCTTCG 
TTTGCACACT TTGAAACAAT TGATCTGTCT CAAGTTGTCT TGGCTGAAAG TAGCTGCAGA 
AATCTTTGCC ACGGTTTTTG TGTTATCACA CCACATCGAA AAGTATCTCT AGTTGCACCC 
ACTCGGCAAG ACATGGAAGA ATGGATTAAC ATCATAAAAA CCGTCCAACA AGGAGAAATC 
TGTCAGATCC CTGCAGCCGA AAACAACCCC TTTCTTGTCG GAATGCACTA CTGGTATTCC 
AGTAATAATC CCCGGAGTCA GTTCTGCAAT GTTTGTCGAG AGAACATTCC TGCTTTATCT 
CGAGATGCCG TCACCTGTGA AGTCTGTCAG GTGAAATCTC ATAAATTCTG TGCTTTGAGA 
GCAAACAAAG ACTGTAAATG GAATACTTTA TCCGTGACTG ATGACCTTCT TCTACCCGCT 
GATGAAATAC AAACAATGCC GCATCAGTGG GTAGAAGGAA ATATACCCGC TGGCTCTCAA 
TGTGTCGTAT GTCACAAGAG CTGCGGTAGT CATCACAGAC TTCAGGATTT CCGCTGCCTC 
TGGTGCGGCT CTACGGTGCA CGGGGCCTGC CAGAAGCGGT TTTCCAAGGA ATGTTCCTTC 
GGAAGTCGTC GCTCGTCCAT CGTGCCACCA ACGGCACTGA GCGACCCTAG GGGCGATGGC 
CAGCTAGTAG TATCGCCTGA CTTCTGGAAT CTCGACTGGC CACTGACTTG TTCCTGTCCC 
TTGCTCATCT TCATCAACTC TAAAAGTGGA GATCATCAAG GGATCATCTT TCTCCGGAAA 
TTCAAACAAT ACCTTAATCC GTCTCAAGTA TTCGACCTGG CGAAGGGTGG ACCTGAAGCA 
GGCATTGCTA TGTTCAAGAA CTTTGCCCGT TTTCGTGTTC TGGTTTGTGG TGGAGATGGC 
AGTGTGAGCT GGGTCTTGTC TACAATTGAT GCCTATGGAT TGCATGATAG GTGTCAGCTG 
GCGATCATCC CACTTGGAAC TGGTAATGAT CTCGCTCGTG TCCTTGGCTG GGGAGCATTC 
TGGAGTAAAG GCACATCACC ACTGGATATT CTCAGCAGAG TGGAGCAGGC TCACGTGAGG 
ATTTTAGACA GATGGAGTGT GATGATCCGT GAGACCCCCA GACAAGCCCC ACTGCTGAAA 
GGACAAGTGG CAATGGATAT ACCAAGATTT GAGGCTGCTG CCATCAAGAA CGTAGAGTCT 
GCAACCACTG AGTTGAACAA AATTCTGAAG GCCAAATATC CCACCGAGAT GGTCATCGCC 
ACAAGATTCC TGTGCTCAGC AGTGGAAGAT TTTGTGGAAG ATATTGTAAA GGCCTGGCAC 
CAAATAAAAC AGAACAGCAC AGCAGTGGAG TCTGTGATTT TGAAAAGTGA CTTAATGTAT 
GATAAACTCA GTGTCCTTAT TGATCTCCTG GCTGAGGATG CAGTAGCTGC CTCCGCTGAG 
AGGACAGCCA CAGCATATGG AAGCAGAAGC AAAGCAGATG GAAAACCCTT CGTGCCTAAA 
CTAGACCACA TAGCAAGGGC CAAACTGGAG TTGGCTGAAA GGGCTCAGAA ACTCCAGCAA 
TCCTTGAAAC TCATCATATT CCAGGTCGAA CAAGTTCTGG ATGAAGAAAG CAGACAGAGC 
TTATCCGTTA AGAACTTTAC TTCATCTTTG TTCCTGGGAG ACGGAGACGA CGACTCAGAT 
GACTATGATC AGAGCCCTAG ACACCGTTCT CGTTGCGACA TCTTATGTTC TATACCTTCT 
TTGAGAAGTG AAGACCTAGA TAACCTTGAC TTAGAACACT TACACATTGC ACCTGAAACG 
ATACGCTTCA AAGAAAAGTG TGTCATGAAC AACTACTTTG GAATTGGACT AGATGCAAAA 
ATCTCTCTGG AGTTTAATTC CAGAAGAGAA GAACACCCAG AACAATACAA TAGCCGCCTC 
AAGAACAAAA TATGGTATGG TCTTCTGGGA AGCAAGGAAG TACTGCAACG CTCTTACAGG 
AAATTGGAAG AGCGAATACA CCTCGAGTGT GATGGAGAAC CTGTCTCCTT GCCAAACCTT 
CAAGGCATCG TAGTGCTCAA CATTACGAGC TACGCTGGAG GCGTCAACTT TTGGGGAAGG 
AACAGAGCAA CCACAGAATA TGATGTCCCT GCAATCAATG ATGGGAAGCT AGAGGTTGTG 
GCAATCTTTG GTTCTGTGCA GATGGCCATG TCTCGTATTG TCAACCTGCA ACAACATCGA 
ATTGCGCAGT GCCATGAGGT GGTAATAACT ATTGATGGTG AAGACGGTGT CCCTGTGCAA 
GTGGATGGGG AAGCCTGGAT TCAGAAGCCA GGCCTTATCA AGATTAAATA CAAGAATGTT 
GCCCAGATGT TGATGAGAGA TAGGGACTTT GAGAACTCAA TGAAAAACTG GGAATCTAAG 
CATACTGAAA TCCAAGCTGT CCAACCACCC CACTTGGATT TCCAGGAATC TCAAGACAGC 
CTCTCTGATG GAGAGTATGC CCAGATGCAG CACTTGGCTC GGCTTGCAGA AAACCTCATT 
AGCAGACTTA CTGACCTAAG CAAGGTCCAC CAGCACGTGT CTGTCCTCAT GGATTCTGTG 
AATGCCAGTG CTAGCATACT GAATGATGTG TTCTATAGCC AAGACAGTGA TACCGAAGCA 
GGTGCAGCTT CCTGCATTCC CATTGAGACT CTAAGCAGAA CTGATGCAGT AGATGTTACA 
TTTAGTCTTA AAGGGCTCTA CGATGACACC AAAGCTTTCC TGGATGAAAA CTTGTTGAGA 
GATGCTGAGG ATCGGGACAT GCTACAAACT GCCTTGGATG CCATGAATAC AGAGTTAAGA 
AGGATACTTG CAATTGGCTG GTTGAGTCAA ATCTTCTTTC CAGAGGAACA GGCTTCTGAC 
ACTCGCAGTT TAAGTCGTAG GTTTAGAATG AAATTCCCCA AGCTGGGGAA GAAGAAACAA 
CGAGAAGAGG GAGAAAAGCC TAAATCAAGC CAAAGATTCC CTGGTTTTCT TGGCAAGTTC 
TGGCGTCGCA GAAATCGTAG CAATCAAGCA AAAGCTGATG ACCCTCCAAC ACCTTCAAGT 
TCTCAACTAT AA