ORF » Species Summary » Medicago truncatula » LOC25502892 cDNA ORF clone

LOC25502892 cDNA ORF clone, Medicago truncatula(barrel medic)

The following LOC25502892 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC25502892 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMe166363 XM_013591012.1
Latest version!		 Medicago truncatula replication factor-A carboxy-terminal domain protein partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
OMe269674 XM_024773093.1
Latest version!		 Medicago truncatula replication protein A 70 kDa DNA-binding subunit E (LOC25502892), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    LOC25502892 ( XM_013591012.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_013446466.1 Medicago truncatula replication factor-A carboxy-terminal domain protein partial mRNA.
    Price & Availability↑
    CloneID OMe166363
    Clone ID Related Accession (Same CDS sequence) XM_013591012.1
    Accession Version XM_013591012.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1668bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-08-24
    Organism Medicago truncatula(barrel medic)
    Product replication factor-A carboxy-terminal domain protein
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_016414). ##Genome-Annotation-Data-START## Annotation Provider :: JCVI Annotation Version :: Mt4.0v2 Annotation Pipeline :: JCVI Eukaryotic Genome Annotation Pipeline Annotation Method :: EVidenceModeler (EVM), PASA Features Annotated :: Gene; mRNA; CDS; tRNA URL :: http://www.jcvi.org/medicago ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTCTAACA CATCTGATTT TTCAGCAATT TCTGAGATTT GCACTGAAAA AACTTTTTGG 
    AACTTGAAGG GTAAAATCAT TAGGCTTTGG CAAGTGTCTG ATTTCAATGG AAATAGGATG 
    CCTTTTTCCA TTGAAATGGT CATTCTTGAT GAGGCTGGTG ATAGGATACA TGCCTCACTC 
    AAGAAGACTC TGATTTACAA GTTTAGGAAT GATCTCTTTG AAGGAAAATG CTTTAGTTTT 
    GAAAATATGG GTGTTGCCAA CAATGGTGGT GGTTATAGAA CCACTAAGCA CACCTACAAG 
    TTGAACTTTC AGTTAAGTTC AAAGGTTAAA TACCTCCCTG CCCTTACAAT CACTAAGTCA 
    CCTTATCAGT TGGTTCCAAT CTCTGAAGTT ATTGGAGGAA CCTATGACAC TGATTACTTA 
    GTTGACATCA TTGGTGTCTT GACTGGTGTT GGAAGTGAGA GGGAACTTGA GAGGAATGGA 
    AATATGACAA AGCTCAATGT CATTGAACTT GAGGCTGGAG GGTTCAAGAT CCAATGTACT 
    TTGTTTGGAA GTTATGTAGA TGATCTCAAC AGCTTTATAG CAAGTGGAGA TATCCCAAAT 
    GCGATTGTGA TCATCCAACT TGCCAAAGCA AAAAGCTTCC AAGATAAAAT ACATGTTCAG 
    AACTGTATTG GTTGCACCAA AGTTATTTTC AAGCCTACAT GTGATGCTGC TGTTAGCATG 
    AGGAATAGGC TGAGTGAGAC TGTTGATACC CCATCCCCAC TTGGTTTAAC CCAGCTGCAA 
    CCAGAAGCAA AACTTGACCC TAGAGAAGAG TTTTTGTATG GAACACCCAG GAACACTATG 
    CAGGGGCTCA AAGATGCTAC CACAAATGAA GTTTATGTTG TGCTTGGAAC CATTAAGAGA 
    ATAGTTAATA GGGAAAATTT TTGGTATACT GCATGTATAT GCTCTAAGGC TGTCATCCCT 
    GATTCTCAAA TGTTCTATTG TGAGAAGTGT GATAGGCATG TGAAGAAAGT ATTCTCTAGG 
    TACTGTTTGA GGGTGAGAGC AATTGATCAT ACAGATTGTG CTACCTTAGT AATTTTTGAC 
    AAAGAAGCAA CTGCACTTTT TAACAAGAGC TGTGTTGACA TGCTAGCTGA ACATGGAGTG 
    GCTGTTTCTG AAGGTCATCT ACCACCTGAA ATTGCTGGAA TCATTGGCAA AACTTTCCTC 
    TTCAAGGTAG AGACCAAAGT TGATCAAAAC CCCAGATTTG AGCAATCATT CAGGGTGAGG 
    AAGATTTGTG CCATGCCGGA TGTTATCAAT GAGTTCAAGA AGAAATGGGG TGAAGAAGAA 
    GCTGCCTTCT TCAAAAACGC CATGGAAGCA TCTTCCTTAA GTGTTTTGTT GGACAAGGGG 
    AAAGCTCCCA TGATTGCTGG CTCTTCTGAT GTGTTGAATC AAGATGATTT TTCATTGACT 
    GAACCTGTTG AGAAGTGCAA GGAAATGCTG TTGGGGGAGG GTTCTGGCAT TGTCACACAG 
    GATCTTCTGC CTAAGTTTGC AGTTGCAGAT TGTGAGTTTG ATGTGGTTGA GATAAGTCAG 
    AAAGGTTCTG CTGCATCTAG CAAGAGGGGA TCTCCCACTA CGGATGATGA TGAGATGAAC 
    ATGTCTCTCA AGATGTTGAG GAAAACTATC AAGATTGAAA AGCCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013446466.1
    CDS1..1668
    Translation

    Target ORF information:

    RefSeq Version XM_013591012.1
    Organism Medicago truncatula(barrel medic)
    Definition Medicago truncatula replication factor-A carboxy-terminal domain protein partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_013591012.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTCTAACA CATCTGATTT TTCAGCAATT TCTGAGATTT GCACTGAAAA AACTTTTTGG 
    AACTTGAAGG GTAAAATCAT TAGGCTTTGG CAAGTGTCTG ATTTCAATGG AAATAGGATG 
    CCTTTTTCCA TTGAAATGGT CATTCTTGAT GAGGCTGGTG ATAGGATACA TGCCTCACTC 
    AAGAAGACTC TGATTTACAA GTTTAGGAAT GATCTCTTTG AAGGAAAATG CTTTAGTTTT 
    GAAAATATGG GTGTTGCCAA CAATGGTGGT GGTTATAGAA CCACTAAGCA CACCTACAAG 
    TTGAACTTTC AGTTAAGTTC AAAGGTTAAA TACCTCCCTG CCCTTACAAT CACTAAGTCA 
    CCTTATCAGT TGGTTCCAAT CTCTGAAGTT ATTGGAGGAA CCTATGACAC TGATTACTTA 
    GTTGACATCA TTGGTGTCTT GACTGGTGTT GGAAGTGAGA GGGAACTTGA GAGGAATGGA 
    AATATGACAA AGCTCAATGT CATTGAACTT GAGGCTGGAG GGTTCAAGAT CCAATGTACT 
    TTGTTTGGAA GTTATGTAGA TGATCTCAAC AGCTTTATAG CAAGTGGAGA TATCCCAAAT 
    GCGATTGTGA TCATCCAACT TGCCAAAGCA AAAAGCTTCC AAGATAAAAT ACATGTTCAG 
    AACTGTATTG GTTGCACCAA AGTTATTTTC AAGCCTACAT GTGATGCTGC TGTTAGCATG 
    AGGAATAGGC TGAGTGAGAC TGTTGATACC CCATCCCCAC TTGGTTTAAC CCAGCTGCAA 
    CCAGAAGCAA AACTTGACCC TAGAGAAGAG TTTTTGTATG GAACACCCAG GAACACTATG 
    CAGGGGCTCA AAGATGCTAC CACAAATGAA GTTTATGTTG TGCTTGGAAC CATTAAGAGA 
    ATAGTTAATA GGGAAAATTT TTGGTATACT GCATGTATAT GCTCTAAGGC TGTCATCCCT 
    GATTCTCAAA TGTTCTATTG TGAGAAGTGT GATAGGCATG TGAAGAAAGT ATTCTCTAGG 
    TACTGTTTGA GGGTGAGAGC AATTGATCAT ACAGATTGTG CTACCTTAGT AATTTTTGAC 
    AAAGAAGCAA CTGCACTTTT TAACAAGAGC TGTGTTGACA TGCTAGCTGA ACATGGAGTG 
    GCTGTTTCTG AAGGTCATCT ACCACCTGAA ATTGCTGGAA TCATTGGCAA AACTTTCCTC 
    TTCAAGGTAG AGACCAAAGT TGATCAAAAC CCCAGATTTG AGCAATCATT CAGGGTGAGG 
    AAGATTTGTG CCATGCCGGA TGTTATCAAT GAGTTCAAGA AGAAATGGGG TGAAGAAGAA 
    GCTGCCTTCT TCAAAAACGC CATGGAAGCA TCTTCCTTAA GTGTTTTGTT GGACAAGGGG 
    AAAGCTCCCA TGATTGCTGG CTCTTCTGAT GTGTTGAATC AAGATGATTT TTCATTGACT 
    GAACCTGTTG AGAAGTGCAA GGAAATGCTG TTGGGGGAGG GTTCTGGCAT TGTCACACAG 
    GATCTTCTGC CTAAGTTTGC AGTTGCAGAT TGTGAGTTTG ATGTGGTTGA GATAAGTCAG 
    AAAGGTTCTG CTGCATCTAG CAAGAGGGGA TCTCCCACTA CGGATGATGA TGAGATGAAC 
    ATGTCTCTCA AGATGTTGAG GAAAACTATC AAGATTGAAA AGCCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LOC25502892 ( XM_024773093.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_024628861.1 Medicago truncatula replication protein A 70 kDa DNA-binding subunit E (LOC25502892), mRNA.
    Price & Availability↑
    CloneID OMe269674
    Clone ID Related Accession (Same CDS sequence) XM_024773093.1
    Accession Version XM_024773093.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1557bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-22
    Organism Medicago truncatula(barrel medic)
    Product replication protein A 70 kDa DNA-binding subunit E
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016414.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases ##RefSeq-Attributes-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGCCTTTTT CCATTGAAAT GGTCATTCTT GATGAGGCTG GTGATAGGAT ACATGCCTCA 
    CTCAAGAAGA CTCTGATTTA CAAGTTTAGG AATGATCTCT TTGAAGGAAA ATGCTTTAGT 
    TTTGAAAATA TGGGTGTTGC CAACAATGGT GGTGGTTATA GAACCACTAA GCACACCTAC 
    AAGTTGAACT TTCAGTTAAG TTCAAAGGTT AAATACCTCC CTGCCCTTAC AATCACTAAG 
    TCACCTTATC AGTTGGTTCC AATCTCTGAA GTTATTGGAG GAACCTATGA CACTGATTAC 
    TTAGTTGTTT TAGACATCAT TGGTGTCTTG ACTGGTGTTG GAAGTGAGAG GGAACTTGAG 
    AGGAATGGAA ATATGACAAA GCTCAATGTC ATTGAACTTG AGGCTGGAGG GTTCAAGATC 
    CAATGTACTT TGTTTGGAAG TTATGTAGAT GATCTCAACA GCTTTATAGC AAGTGGAGAT 
    ATCCCAAATG CGATTGTGAT CATCCAACTT GCCAAAGCAA AAAGCTTCCA AGATAAAATA 
    CATGTTCAGA ACTGTATTGG TTGCACCAAA GTTATTTTCA AGCCTACATG TGATGCTGCT 
    GTTAGCATGA GGAATAGGCT GAGTGAGACT GTTGATACCC CATCCCCACT TGGTTTAACC 
    CAGCTGCAAC CAGAAGCAAA ACTTGACCCT AGAGAAGAGT TTTTGTATGG AACACCCAGG 
    AACACTATGC AGGGGCTCAA AGATGCTACC ACAAATGAAG TTTATGTTGT GCTTGGAACC 
    ATTAAGAGAA TAGTTAATAG GGAAAATTTT TGGTATACTG CATGTATATG CTCTAAGGCT 
    GTCATCCCTG ATTCTCAAAT GTTCTATTGT GAGAAGTGTG ATAGGCATGT GAAGAAAGTA 
    TTCTCTAGGT ACTGTTTGAG GGTGAGAGCA ATTGATCATA CAGATTGTGC TACCTTAGTA 
    ATTTTTGACA AAGAAGCAAC TGCACTTTTT AACAAGAGCT GTGTTGACAT GCTAGCTGAA 
    CATGGAGTGG CTGTTTCTGA AGGTCATCTA CCACCTGAAA TTGCTGGAAT CATTGGCAAA 
    ACTTTCCTCT TCAAGGTAGA GACCAAAGTT GATCAAAACC CCAGATTTGA GCAATCATTC 
    AGGGTGAGGA AGATTTGTGC CATGCCGGAT GTTATCAATG AGTTCAAGAA GAAATGGGGT 
    GAAGAAGAAG CTGCCTTCTT CAAAAACGCC ATGGAAGCAT CTTCCTTAAG TGTTTTGTTG 
    GACAAGGGGA AAGCTCCCAT GATTGCTGGC TCTTCTGATG TGTTGAATCA AGATGATTTT 
    TCATTGACTG AACCTGTTGA GAAGTGCAAG GAAATGCTGT TGGGGGAGGG TTCTGGCATT 
    GTCACACAGG ATCTTCTGCC TAAGTTTGCA GTTGCAGATT GTGAGTTTGA TGTGGTTGAG 
    ATAAGTCAGA AAGGTTCTGC TGCATCTAGC AAGAGGGGAT CTCCCACTAC GGATGATGAT 
    GAGATGAACA TGTCTCTCAA GATGTTGAGG AAAACTATCA AGATTGAAAA GCCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024628861.1
    CDS38..1594
    Translation

    Target ORF information:

    RefSeq Version XM_024773093.1
    Organism Medicago truncatula(barrel medic)
    Definition Medicago truncatula replication protein A 70 kDa DNA-binding subunit E (LOC25502892), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024773093.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGCCTTTTT CCATTGAAAT GGTCATTCTT GATGAGGCTG GTGATAGGAT ACATGCCTCA 
    CTCAAGAAGA CTCTGATTTA CAAGTTTAGG AATGATCTCT TTGAAGGAAA ATGCTTTAGT 
    TTTGAAAATA TGGGTGTTGC CAACAATGGT GGTGGTTATA GAACCACTAA GCACACCTAC 
    AAGTTGAACT TTCAGTTAAG TTCAAAGGTT AAATACCTCC CTGCCCTTAC AATCACTAAG 
    TCACCTTATC AGTTGGTTCC AATCTCTGAA GTTATTGGAG GAACCTATGA CACTGATTAC 
    TTAGTTGTTT TAGACATCAT TGGTGTCTTG ACTGGTGTTG GAAGTGAGAG GGAACTTGAG 
    AGGAATGGAA ATATGACAAA GCTCAATGTC ATTGAACTTG AGGCTGGAGG GTTCAAGATC 
    CAATGTACTT TGTTTGGAAG TTATGTAGAT GATCTCAACA GCTTTATAGC AAGTGGAGAT 
    ATCCCAAATG CGATTGTGAT CATCCAACTT GCCAAAGCAA AAAGCTTCCA AGATAAAATA 
    CATGTTCAGA ACTGTATTGG TTGCACCAAA GTTATTTTCA AGCCTACATG TGATGCTGCT 
    GTTAGCATGA GGAATAGGCT GAGTGAGACT GTTGATACCC CATCCCCACT TGGTTTAACC 
    CAGCTGCAAC CAGAAGCAAA ACTTGACCCT AGAGAAGAGT TTTTGTATGG AACACCCAGG 
    AACACTATGC AGGGGCTCAA AGATGCTACC ACAAATGAAG TTTATGTTGT GCTTGGAACC 
    ATTAAGAGAA TAGTTAATAG GGAAAATTTT TGGTATACTG CATGTATATG CTCTAAGGCT 
    GTCATCCCTG ATTCTCAAAT GTTCTATTGT GAGAAGTGTG ATAGGCATGT GAAGAAAGTA 
    TTCTCTAGGT ACTGTTTGAG GGTGAGAGCA ATTGATCATA CAGATTGTGC TACCTTAGTA 
    ATTTTTGACA AAGAAGCAAC TGCACTTTTT AACAAGAGCT GTGTTGACAT GCTAGCTGAA 
    CATGGAGTGG CTGTTTCTGA AGGTCATCTA CCACCTGAAA TTGCTGGAAT CATTGGCAAA 
    ACTTTCCTCT TCAAGGTAGA GACCAAAGTT GATCAAAACC CCAGATTTGA GCAATCATTC 
    AGGGTGAGGA AGATTTGTGC CATGCCGGAT GTTATCAATG AGTTCAAGAA GAAATGGGGT 
    GAAGAAGAAG CTGCCTTCTT CAAAAACGCC ATGGAAGCAT CTTCCTTAAG TGTTTTGTTG 
    GACAAGGGGA AAGCTCCCAT GATTGCTGGC TCTTCTGATG TGTTGAATCA AGATGATTTT 
    TCATTGACTG AACCTGTTGA GAAGTGCAAG GAAATGCTGT TGGGGGAGGG TTCTGGCATT 
    GTCACACAGG ATCTTCTGCC TAAGTTTGCA GTTGCAGATT GTGAGTTTGA TGTGGTTGAG 
    ATAAGTCAGA AAGGTTCTGC TGCATCTAGC AAGAGGGGAT CTCCCACTAC GGATGATGAT 
    GAGATGAACA TGTCTCTCAA GATGTTGAGG AAAACTATCA AGATTGAAAA GCCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
    BMC genomics15312(2014 Apr)
    Tang H,Krishnakumar V,Bidwell S,Rosen B,Chan A,Zhou S,Gentzbittel L,Childs KL,Yandell M,Gundlach H,Mayer KF,Schwartz DC,Town CD


    The Medicago genome provides insight into the evolution of rhizobial symbioses.
    Nature480(7378)520-4(2011 Nov)
    Young ND,Debell? F,Oldroyd GE,Geurts R,Cannon SB,Udvardi MK,Benedito VA,Mayer KF,Gouzy J,Schoof H,Van de Peer Y,Proost S,Cook DR,Meyers BC,Spannagl M,Cheung F,De Mita S,Krishnakumar V,Gundlach H,Zhou S,Mudge J,Bharti AK,Murray JD,Naoumkina MA,Rosen B,Silverstein KA,Tang H,Rombauts S,Zhao PX,Zhou P,Barbe V,Bardou P,Bechner M,Bellec A,Berger A,Berg?s H,Bidwell S,Bisseling T,Choisne N,Couloux A,Denny R,Deshpande S,Dai X,Doyle JJ,Dudez AM,Farmer AD,Fouteau S,Franken C,Gibelin C,Gish J,Goldstein S,Gonz?lez AJ,Green PJ,Hallab A,Hartog M,Hua A,Humphray SJ,Jeong DH,Jing Y,J?cker A,Kenton SM,Kim DJ,Klee K,Lai H,Lang C,Lin S,Macmil SL,Magdelenat G,Matthews L,McCorrison J,Monaghan EL,Mun JH,Najar FZ,Nicholson C,Noirot C,O'Bleness M,Paule CR,Poulain J,Prion F,Qin B,Qu C,Retzel EF,Riddle C,Sallet E,Samain S,Samson N,Sanders I,Saurat O,Scarpelli C,Schiex T,Segurens B,Severin AJ,Sherrier DJ,Shi R,Sims S,Singer SR,Sinharoy S,Sterck L,Viollet A,Wang BB,Wang K,Wang M,Wang X,Warfsmann J,Weissenbach J,White DD,White JD,Wiley GB,Wincker P,Xing Y,Yang L,Yao Z,Ying F,Zhai J,Zhou L,Zuber A,D?nari? J,Dixon RA,May GD,Schwartz DC,Rogers J,Qu?tier F,Town CD,Roe BA


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