MTR_6g004880 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene
Clones
gRNAs

Summary

Gene Symbol	MTR_6g004880
Entrez Gene ID	25495142
Full Name	raffinose synthase or seed inhibition protein
Gene Type	protein-coding
Organism	Medicago truncatula(barrel medic)

mRNA and Protein(s)

mRNA	Protein	Name
XM_013595310.2	XP_013450764.1	galactinol--sucrose galactosyltransferase
XM_013595310.1	XP_013450764.1	raffinose synthase or seed inhibition protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
Tang H, Krishnakumar V, Bidwell S, Rosen B, Chan A, Zhou S, Gentzbittel L, Childs KL, Yandell M, Gundlach H, Mayer KF, Schwartz DC, Town CD
BMC genomics15312(2014 Apr)

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Young ND, Debell? F, Oldroyd GE, Geurts R, Cannon SB, Udvardi MK, Benedito VA, Mayer KF, Gouzy J, Schoof H, Van de Peer Y, Proost S, Cook DR, Meyers BC, Spannagl M, Cheung F, De Mita S, Krishnakumar V, Gundlach H, Zhou S, Mudge J, Bharti AK, Murray JD, Naoumkina MA, Rosen B, Silverstein KA, Tang H, Rombauts S, Zhao PX, Zhou P, Barbe V, Bardou P, Bechner M, Bellec A, Berger A, Berg?s H, Bidwell S, Bisseling T, Choisne N, Couloux A, Denny R, Deshpande S, Dai X, Doyle JJ, Dudez AM, Farmer AD, Fouteau S, Franken C, Gibelin C, Gish J, Goldstein S, Gonz?lez AJ, Green PJ, Hallab A, Hartog M, Hua A, Humphray SJ, Jeong DH, Jing Y, J?cker A, Kenton SM, Kim DJ, Klee K, Lai H, Lang C, Lin S, Macmil SL, Magdelenat G, Matthews L, McCorrison J, Monaghan EL, Mun JH, Najar FZ, Nicholson C, Noirot C, O'Bleness M, Paule CR, Poulain J, Prion F, Qin B, Qu C, Retzel EF, Riddle C, Sallet E, Samain S, Samson N, Sanders I, Saurat O, Scarpelli C, Schiex T, Segurens B, Severin AJ, Sherrier DJ, Shi R, Sims S, Singer SR, Sinharoy S, Sterck L, Viollet A, Wang BB, Wang K, Wang M, Wang X, Warfsmann J, Weissenbach J, White DD, White JD, Wiley GB, Wincker P, Xing Y, Yang L, Yao Z, Ying F, Zhai J, Zhou L, Zuber A, D?nari? J, Dixon RA, May GD, Schwartz DC, Rogers J, Qu?tier F, Town CD, Roe BA
Nature480(7378)520-4(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MTR_6g004880 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MTR_6g004880 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe174752	XM_013595310.2 Latest version!	Medicago truncatula galactinol--sucrose galactosyltransferase (LOC25495142), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OMe174752	XM_013595310.1	Medicago truncatula raffinose synthase or seed inhibition protein mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MTR_6g004880 ( XM_013595310.2 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_013450764.1 Medicago truncatula galactinol--sucrose galactosyltransferase (LOC25495142), mRNA.

Price & Availability↑

CloneID	OMe174752
Clone ID Related Accession (Same CDS sequence)	XM_013595310.2 , XM_013595310.1
Accession Version	XM_013595310.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	galactinol--sucrose galactosyltransferase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016412.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2018 this sequence version replaced XM_013595310.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCCTC CAAACCCTAC CCTAAACTCA ACCAGAAACA ACACCGACAA CATTATCACC 
ACCACCAAAC ATGTTAACCC TTGTCCCAAT TCACCCTTCA CCTTCACCCT ATCCGACTCA 
ACTCTAAAAG TTAACAACCA CGTCATCCTC TCTGATGTCC CCAAAAACAT CACCACCACT 
ACCACTCCGT CCACCACCGT CACCGCAACC AACGGTTGCT TCCTTGGTTT TGAAGCCAAC 
GAAGCGAAAA GCCGCCATGT TGCACACATT GGAAAGCTCA AAAACATAAA CTTTACTAGC 
ATTTTCCGAT TTAAAGTGTG GTGGACCACT CTTTGGACCG GTTCAAATGG CAAAGACTTA 
GAAACCGAGA CCCAATTCCT CATGCTCCAG AACTCGGATC CAAATCCGGG TTCAGATCCA 
AACTCTGGTC GACCCTATGT TCTATTACTT CCAATAATAG AAGGGTCTTT CCGAGCTTCT 
TTACAACCCG GTGATGACGA CAACGTTGCT GTCTGTGTCG AGAGTAGTTC AACTTTAGTA 
ACTGACTCTC GATACAACGC TGTTGTTTAC CTTCACGCGG GTAATGATCC ATTTAAGCTT 
GTGAAAGAAG CAATGAAAAC TGTGAGGGCC CACTTAGGAA CCTTTAAGCT TCTAGAAGAA 
AAAACTGTCC CCGGAATAGT GGATAAATTT GGATGGTGTA CATGGGATGC ATTTTATCTT 
AAGGTACACC CTCAAGGAGT TTGGGAAGGT GTTAAGGATT TATCTGATGG TGGTTGTCCA 
CCTGGCTTTG TCTTGATTGA TGATGGTTGG CAATCTATAG CCCATGATGA TGATCCAATT 
GATACTAATG ATGGGATTAA TCGTACGGCT GCAGGTGAAC AAATGCCATG TAGGTTAATA 
AAATTTGAAG AGAATTGTAA GTTTAAGGAT TATAAAAAAG GTTTAGGGTT AGGTGGATTT 
GTTAAGGAAC TAAAAAAAGG TTTTGAGAGT GTTGAATATG TGTATGTGTG GCATGCTTTA 
TGTGGTTATT GGGGTGGGAT AAGACCTAAT GTTGAGGGTC TACCTGAAGC TATTGTGGAG 
AAGCCTAAGT TAACCGTTGG ATTGGAAACT ACTATGGAGG ATTTGGCTGT TGATAAGATT 
GTTAATAATG GTGTTGGGTT AGTTCCTCCT CATTTGGTTG ATCAAATGTA TGAAGGTATT 
CACTCTCTTT TGGAAGAGTC TGGAATTGAT GGGGTCAAGG TTGATGTCAT CCATTTGCTA 
GAGATGGTGG GTGAGAAGTA TGGTGGAAGA GTTGATTTGG CCAAAGCATA TTACAAAGCT 
CTCTCAACTT CAGTGAAAAA ACATTTCAAT GGTAATGGTG TCATTGCTAG CATGGAACAT 
TGCAATGATT TCATGTTGCT TGGAACTGAA GCCATATCCC TTGGTCGTGT TGGTGATGAT 
TTCTGGTGCA CTGACCCATA TGGTGATCCA AATGGTACAT ATTGGCTACA AGGATGTCAC 
ATGGTGCATT GTGCATACAA TAGCTTATGG ATGGGAAATT TCATCCAACC AGATTGGGAT 
ATGTTTCAAT CTACACACCC TTGTGCTGCT TTTCATGCAG CTTCTAGAGC CATATCTGGT 
GGTCCAATTT ACATAAGTGA CACTGTTGGA AATCACAATT TTGACCTTCT CAAGAAACTA 
GCTTTGCCTG ATGGTACCAT CCTTAGATGT GAAACCTATG CTCTCCCAAC TAAGGACTGT 
CTCTTTTCTG ATCCTTTACA TGATGGCAAA ACTATGCTCA AAATTTGGAA CCTCAATAAG 
TACACTGGAG TTCTTGGTGT GTTTAACTGC CAAGGAGGAG GATGGTTTCG CGAGATAAGA 
TCAAACAAAT GTGCTTCTGA GTTTTCACAT TCGGTATCAA CTAAGATCAA CATCAAAGAC 
ATTGAATGGA ACAGTGGAAA GAATCCAATT CCCATTGAAG GAGTTCAAGT TTTTGCTTTG 
TATTTCAGCC AAGCCAAGAA GCTGAAACTT TTCTCTCCAT CTGAAATTGA AGAAATCTCT 
TTGGAGCCAT TCAACTTTGA GCTTATAACT GTTTCTCCAG TCACTTTATT TCCTAAAAAG 
TCCTTGAAGT TTGCTCCTAT TGGTTTGGTT AACATGCTAA ACAATGGTGG GGCAATTCAG 
TCATTTGAAT ATCTTGAGGC TCAAGATTTG GTGCAAGTTG GAATTAGAGG TGCTGGTGAG 
ATGAGGGTCT ATGCTTCAGA GAAACCAAGG GCTTGTGGAA TTGATGGCAA AGAAGTTGAT 
TTTGAATATA AAGAGTTCAT GGTTGTGATT CAAGTACCAT GGCCTAGTTC TTCAAAATGG 
TCCTTTGTTC AGTATATATT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013450764.1
CDS	282..2645
Translation	MSPPNPTLNSTRNNTDNIITTTKHVNPCPNSPFTFTLSDSTLKVNNHVILSDVPKNITTTTTPSTTVTATNGCFLGFEANEAKSRHVAHIGKLKNINFTSIFRFKVWWTTLWTGSNGKDLETETQFLMLQNSDPNPGSDPNSGRPYVLLLPIIEGSFRASLQPGDDDNVAVCVESSSTLVTDSRYNAVVYLHAGNDPFKLVKEAMKTVRAHLGTFKLLEEKTVPGIVDKFGWCTWDAFYLKVHPQGVWEGVKDLSDGGCPPGFVLIDDGWQSIAHDDDPIDTNDGINRTAAGEQMPCRLIKFEENCKFKDYKKGLGLGGFVKELKKGFESVEYVYVWHALCGYWGGIRPNVEGLPEAIVEKPKLTVGLETTMEDLAVDKIVNNGVGLVPPHLVDQMYEGIHSLLEESGIDGVKVDVIHLLEMVGEKYGGRVDLAKAYYKALSTSVKKHFNGNGVIASMEHCNDFMLLGTEAISLGRVGDDFWCTDPYGDPNGTYWLQGCHMVHCAYNSLWMGNFIQPDWDMFQSTHPCAAFHAASRAISGGPIYISDTVGNHNFDLLKKLALPDGTILRCETYALPTKDCLFSDPLHDGKTMLKIWNLNKYTGVLGVFNCQGGGWFREIRSNKCASEFSHSVSTKINIKDIEWNSGKNPIPIEGVQVFALYFSQAKKLKLFSPSEIEEISLEPFNFELITVSPVTLFPKKSLKFAPIGLVNMLNNGGAIQSFEYLEAQDLVQVGIRGAGEMRVYASEKPRACGIDGKEVDFEYKEFMVVIQVPWPSSSKWSFVQYIF

Target ORF information:

RefSeq Version	XM_013595310.2
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula galactinol--sucrose galactosyltransferase (LOC25495142), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013595310.2

ORF Insert Sequence:

ATGTCTCCTC CAAACCCTAC CCTAAACTCA ACCAGAAACA ACACCGACAA CATTATCACC 
ACCACCAAAC ATGTTAACCC TTGTCCCAAT TCACCCTTCA CCTTCACCCT ATCCGACTCA 
ACTCTAAAAG TTAACAACCA CGTCATCCTC TCTGATGTCC CCAAAAACAT CACCACCACT 
ACCACTCCGT CCACCACCGT CACCGCAACC AACGGTTGCT TCCTTGGTTT TGAAGCCAAC 
GAAGCGAAAA GCCGCCATGT TGCACACATT GGAAAGCTCA AAAACATAAA CTTTACTAGC 
ATTTTCCGAT TTAAAGTGTG GTGGACCACT CTTTGGACCG GTTCAAATGG CAAAGACTTA 
GAAACCGAGA CCCAATTCCT CATGCTCCAG AACTCGGATC CAAATCCGGG TTCAGATCCA 
AACTCTGGTC GACCCTATGT TCTATTACTT CCAATAATAG AAGGGTCTTT CCGAGCTTCT 
TTACAACCCG GTGATGACGA CAACGTTGCT GTCTGTGTCG AGAGTAGTTC AACTTTAGTA 
ACTGACTCTC GATACAACGC TGTTGTTTAC CTTCACGCGG GTAATGATCC ATTTAAGCTT 
GTGAAAGAAG CAATGAAAAC TGTGAGGGCC CACTTAGGAA CCTTTAAGCT TCTAGAAGAA 
AAAACTGTCC CCGGAATAGT GGATAAATTT GGATGGTGTA CATGGGATGC ATTTTATCTT 
AAGGTACACC CTCAAGGAGT TTGGGAAGGT GTTAAGGATT TATCTGATGG TGGTTGTCCA 
CCTGGCTTTG TCTTGATTGA TGATGGTTGG CAATCTATAG CCCATGATGA TGATCCAATT 
GATACTAATG ATGGGATTAA TCGTACGGCT GCAGGTGAAC AAATGCCATG TAGGTTAATA 
AAATTTGAAG AGAATTGTAA GTTTAAGGAT TATAAAAAAG GTTTAGGGTT AGGTGGATTT 
GTTAAGGAAC TAAAAAAAGG TTTTGAGAGT GTTGAATATG TGTATGTGTG GCATGCTTTA 
TGTGGTTATT GGGGTGGGAT AAGACCTAAT GTTGAGGGTC TACCTGAAGC TATTGTGGAG 
AAGCCTAAGT TAACCGTTGG ATTGGAAACT ACTATGGAGG ATTTGGCTGT TGATAAGATT 
GTTAATAATG GTGTTGGGTT AGTTCCTCCT CATTTGGTTG ATCAAATGTA TGAAGGTATT 
CACTCTCTTT TGGAAGAGTC TGGAATTGAT GGGGTCAAGG TTGATGTCAT CCATTTGCTA 
GAGATGGTGG GTGAGAAGTA TGGTGGAAGA GTTGATTTGG CCAAAGCATA TTACAAAGCT 
CTCTCAACTT CAGTGAAAAA ACATTTCAAT GGTAATGGTG TCATTGCTAG CATGGAACAT 
TGCAATGATT TCATGTTGCT TGGAACTGAA GCCATATCCC TTGGTCGTGT TGGTGATGAT 
TTCTGGTGCA CTGACCCATA TGGTGATCCA AATGGTACAT ATTGGCTACA AGGATGTCAC 
ATGGTGCATT GTGCATACAA TAGCTTATGG ATGGGAAATT TCATCCAACC AGATTGGGAT 
ATGTTTCAAT CTACACACCC TTGTGCTGCT TTTCATGCAG CTTCTAGAGC CATATCTGGT 
GGTCCAATTT ACATAAGTGA CACTGTTGGA AATCACAATT TTGACCTTCT CAAGAAACTA 
GCTTTGCCTG ATGGTACCAT CCTTAGATGT GAAACCTATG CTCTCCCAAC TAAGGACTGT 
CTCTTTTCTG ATCCTTTACA TGATGGCAAA ACTATGCTCA AAATTTGGAA CCTCAATAAG 
TACACTGGAG TTCTTGGTGT GTTTAACTGC CAAGGAGGAG GATGGTTTCG CGAGATAAGA 
TCAAACAAAT GTGCTTCTGA GTTTTCACAT TCGGTATCAA CTAAGATCAA CATCAAAGAC 
ATTGAATGGA ACAGTGGAAA GAATCCAATT CCCATTGAAG GAGTTCAAGT TTTTGCTTTG 
TATTTCAGCC AAGCCAAGAA GCTGAAACTT TTCTCTCCAT CTGAAATTGA AGAAATCTCT 
TTGGAGCCAT TCAACTTTGA GCTTATAACT GTTTCTCCAG TCACTTTATT TCCTAAAAAG 
TCCTTGAAGT TTGCTCCTAT TGGTTTGGTT AACATGCTAA ACAATGGTGG GGCAATTCAG 
TCATTTGAAT ATCTTGAGGC TCAAGATTTG GTGCAAGTTG GAATTAGAGG TGCTGGTGAG 
ATGAGGGTCT ATGCTTCAGA GAAACCAAGG GCTTGTGGAA TTGATGGCAA AGAAGTTGAT 
TTTGAATATA AAGAGTTCAT GGTTGTGATT CAAGTACCAT GGCCTAGTTC TTCAAAATGG 
TCCTTTGTTC AGTATATATT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTR_6g004880 ( XM_013595310.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_013450764.1 Medicago truncatula raffinose synthase or seed inhibition protein mRNA.

Price & Availability↑

CloneID	OMe174752
Clone ID Related Accession (Same CDS sequence)	XM_013595310.2 , XM_013595310.1
Accession Version	XM_013595310.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-24
Organism	Medicago truncatula(barrel medic)
Product	raffinose synthase or seed inhibition protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_016412). ##Genome-Annotation-Data-START## Annotation Provider :: JCVI Annotation Version :: Mt4.0v2 Annotation Pipeline :: JCVI Eukaryotic Genome Annotation Pipeline Annotation Method :: EVidenceModeler (EVM), PASA Features Annotated :: Gene; mRNA; CDS; tRNA URL :: http://www.jcvi.org/medicago ##Genome-Annotation-Data-END##

ATGTCTCCTC CAAACCCTAC CCTAAACTCA ACCAGAAACA ACACCGACAA CATTATCACC 
ACCACCAAAC ATGTTAACCC TTGTCCCAAT TCACCCTTCA CCTTCACCCT ATCCGACTCA 
ACTCTAAAAG TTAACAACCA CGTCATCCTC TCTGATGTCC CCAAAAACAT CACCACCACT 
ACCACTCCGT CCACCACCGT CACCGCAACC AACGGTTGCT TCCTTGGTTT TGAAGCCAAC 
GAAGCGAAAA GCCGCCATGT TGCACACATT GGAAAGCTCA AAAACATAAA CTTTACTAGC 
ATTTTCCGAT TTAAAGTGTG GTGGACCACT CTTTGGACCG GTTCAAATGG CAAAGACTTA 
GAAACCGAGA CCCAATTCCT CATGCTCCAG AACTCGGATC CAAATCCGGG TTCAGATCCA 
AACTCTGGTC GACCCTATGT TCTATTACTT CCAATAATAG AAGGGTCTTT CCGAGCTTCT 
TTACAACCCG GTGATGACGA CAACGTTGCT GTCTGTGTCG AGAGTAGTTC AACTTTAGTA 
ACTGACTCTC GATACAACGC TGTTGTTTAC CTTCACGCGG GTAATGATCC ATTTAAGCTT 
GTGAAAGAAG CAATGAAAAC TGTGAGGGCC CACTTAGGAA CCTTTAAGCT TCTAGAAGAA 
AAAACTGTCC CCGGAATAGT GGATAAATTT GGATGGTGTA CATGGGATGC ATTTTATCTT 
AAGGTACACC CTCAAGGAGT TTGGGAAGGT GTTAAGGATT TATCTGATGG TGGTTGTCCA 
CCTGGCTTTG TCTTGATTGA TGATGGTTGG CAATCTATAG CCCATGATGA TGATCCAATT 
GATACTAATG ATGGGATTAA TCGTACGGCT GCAGGTGAAC AAATGCCATG TAGGTTAATA 
AAATTTGAAG AGAATTGTAA GTTTAAGGAT TATAAAAAAG GTTTAGGGTT AGGTGGATTT 
GTTAAGGAAC TAAAAAAAGG TTTTGAGAGT GTTGAATATG TGTATGTGTG GCATGCTTTA 
TGTGGTTATT GGGGTGGGAT AAGACCTAAT GTTGAGGGTC TACCTGAAGC TATTGTGGAG 
AAGCCTAAGT TAACCGTTGG ATTGGAAACT ACTATGGAGG ATTTGGCTGT TGATAAGATT 
GTTAATAATG GTGTTGGGTT AGTTCCTCCT CATTTGGTTG ATCAAATGTA TGAAGGTATT 
CACTCTCTTT TGGAAGAGTC TGGAATTGAT GGGGTCAAGG TTGATGTCAT CCATTTGCTA 
GAGATGGTGG GTGAGAAGTA TGGTGGAAGA GTTGATTTGG CCAAAGCATA TTACAAAGCT 
CTCTCAACTT CAGTGAAAAA ACATTTCAAT GGTAATGGTG TCATTGCTAG CATGGAACAT 
TGCAATGATT TCATGTTGCT TGGAACTGAA GCCATATCCC TTGGTCGTGT TGGTGATGAT 
TTCTGGTGCA CTGACCCATA TGGTGATCCA AATGGTACAT ATTGGCTACA AGGATGTCAC 
ATGGTGCATT GTGCATACAA TAGCTTATGG ATGGGAAATT TCATCCAACC AGATTGGGAT 
ATGTTTCAAT CTACACACCC TTGTGCTGCT TTTCATGCAG CTTCTAGAGC CATATCTGGT 
GGTCCAATTT ACATAAGTGA CACTGTTGGA AATCACAATT TTGACCTTCT CAAGAAACTA 
GCTTTGCCTG ATGGTACCAT CCTTAGATGT GAAACCTATG CTCTCCCAAC TAAGGACTGT 
CTCTTTTCTG ATCCTTTACA TGATGGCAAA ACTATGCTCA AAATTTGGAA CCTCAATAAG 
TACACTGGAG TTCTTGGTGT GTTTAACTGC CAAGGAGGAG GATGGTTTCG CGAGATAAGA 
TCAAACAAAT GTGCTTCTGA GTTTTCACAT TCGGTATCAA CTAAGATCAA CATCAAAGAC 
ATTGAATGGA ACAGTGGAAA GAATCCAATT CCCATTGAAG GAGTTCAAGT TTTTGCTTTG 
TATTTCAGCC AAGCCAAGAA GCTGAAACTT TTCTCTCCAT CTGAAATTGA AGAAATCTCT 
TTGGAGCCAT TCAACTTTGA GCTTATAACT GTTTCTCCAG TCACTTTATT TCCTAAAAAG 
TCCTTGAAGT TTGCTCCTAT TGGTTTGGTT AACATGCTAA ACAATGGTGG GGCAATTCAG 
TCATTTGAAT ATCTTGAGGC TCAAGATTTG GTGCAAGTTG GAATTAGAGG TGCTGGTGAG 
ATGAGGGTCT ATGCTTCAGA GAAACCAAGG GCTTGTGGAA TTGATGGCAA AGAAGTTGAT 
TTTGAATATA AAGAGTTCAT GGTTGTGATT CAAGTACCAT GGCCTAGTTC TTCAAAATGG 
TCCTTTGTTC AGTATATATT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013450764.1
CDS	276..2639
Translation	MSPPNPTLNSTRNNTDNIITTTKHVNPCPNSPFTFTLSDSTLKVNNHVILSDVPKNITTTTTPSTTVTATNGCFLGFEANEAKSRHVAHIGKLKNINFTSIFRFKVWWTTLWTGSNGKDLETETQFLMLQNSDPNPGSDPNSGRPYVLLLPIIEGSFRASLQPGDDDNVAVCVESSSTLVTDSRYNAVVYLHAGNDPFKLVKEAMKTVRAHLGTFKLLEEKTVPGIVDKFGWCTWDAFYLKVHPQGVWEGVKDLSDGGCPPGFVLIDDGWQSIAHDDDPIDTNDGINRTAAGEQMPCRLIKFEENCKFKDYKKGLGLGGFVKELKKGFESVEYVYVWHALCGYWGGIRPNVEGLPEAIVEKPKLTVGLETTMEDLAVDKIVNNGVGLVPPHLVDQMYEGIHSLLEESGIDGVKVDVIHLLEMVGEKYGGRVDLAKAYYKALSTSVKKHFNGNGVIASMEHCNDFMLLGTEAISLGRVGDDFWCTDPYGDPNGTYWLQGCHMVHCAYNSLWMGNFIQPDWDMFQSTHPCAAFHAASRAISGGPIYISDTVGNHNFDLLKKLALPDGTILRCETYALPTKDCLFSDPLHDGKTMLKIWNLNKYTGVLGVFNCQGGGWFREIRSNKCASEFSHSVSTKINIKDIEWNSGKNPIPIEGVQVFALYFSQAKKLKLFSPSEIEEISLEPFNFELITVSPVTLFPKKSLKFAPIGLVNMLNNGGAIQSFEYLEAQDLVQVGIRGAGEMRVYASEKPRACGIDGKEVDFEYKEFMVVIQVPWPSSSKWSFVQYIF

Target ORF information:

RefSeq Version	XM_013595310.1
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula raffinose synthase or seed inhibition protein mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013595310.1

ORF Insert Sequence:

ATGTCTCCTC CAAACCCTAC CCTAAACTCA ACCAGAAACA ACACCGACAA CATTATCACC 
ACCACCAAAC ATGTTAACCC TTGTCCCAAT TCACCCTTCA CCTTCACCCT ATCCGACTCA 
ACTCTAAAAG TTAACAACCA CGTCATCCTC TCTGATGTCC CCAAAAACAT CACCACCACT 
ACCACTCCGT CCACCACCGT CACCGCAACC AACGGTTGCT TCCTTGGTTT TGAAGCCAAC 
GAAGCGAAAA GCCGCCATGT TGCACACATT GGAAAGCTCA AAAACATAAA CTTTACTAGC 
ATTTTCCGAT TTAAAGTGTG GTGGACCACT CTTTGGACCG GTTCAAATGG CAAAGACTTA 
GAAACCGAGA CCCAATTCCT CATGCTCCAG AACTCGGATC CAAATCCGGG TTCAGATCCA 
AACTCTGGTC GACCCTATGT TCTATTACTT CCAATAATAG AAGGGTCTTT CCGAGCTTCT 
TTACAACCCG GTGATGACGA CAACGTTGCT GTCTGTGTCG AGAGTAGTTC AACTTTAGTA 
ACTGACTCTC GATACAACGC TGTTGTTTAC CTTCACGCGG GTAATGATCC ATTTAAGCTT 
GTGAAAGAAG CAATGAAAAC TGTGAGGGCC CACTTAGGAA CCTTTAAGCT TCTAGAAGAA 
AAAACTGTCC CCGGAATAGT GGATAAATTT GGATGGTGTA CATGGGATGC ATTTTATCTT 
AAGGTACACC CTCAAGGAGT TTGGGAAGGT GTTAAGGATT TATCTGATGG TGGTTGTCCA 
CCTGGCTTTG TCTTGATTGA TGATGGTTGG CAATCTATAG CCCATGATGA TGATCCAATT 
GATACTAATG ATGGGATTAA TCGTACGGCT GCAGGTGAAC AAATGCCATG TAGGTTAATA 
AAATTTGAAG AGAATTGTAA GTTTAAGGAT TATAAAAAAG GTTTAGGGTT AGGTGGATTT 
GTTAAGGAAC TAAAAAAAGG TTTTGAGAGT GTTGAATATG TGTATGTGTG GCATGCTTTA 
TGTGGTTATT GGGGTGGGAT AAGACCTAAT GTTGAGGGTC TACCTGAAGC TATTGTGGAG 
AAGCCTAAGT TAACCGTTGG ATTGGAAACT ACTATGGAGG ATTTGGCTGT TGATAAGATT 
GTTAATAATG GTGTTGGGTT AGTTCCTCCT CATTTGGTTG ATCAAATGTA TGAAGGTATT 
CACTCTCTTT TGGAAGAGTC TGGAATTGAT GGGGTCAAGG TTGATGTCAT CCATTTGCTA 
GAGATGGTGG GTGAGAAGTA TGGTGGAAGA GTTGATTTGG CCAAAGCATA TTACAAAGCT 
CTCTCAACTT CAGTGAAAAA ACATTTCAAT GGTAATGGTG TCATTGCTAG CATGGAACAT 
TGCAATGATT TCATGTTGCT TGGAACTGAA GCCATATCCC TTGGTCGTGT TGGTGATGAT 
TTCTGGTGCA CTGACCCATA TGGTGATCCA AATGGTACAT ATTGGCTACA AGGATGTCAC 
ATGGTGCATT GTGCATACAA TAGCTTATGG ATGGGAAATT TCATCCAACC AGATTGGGAT 
ATGTTTCAAT CTACACACCC TTGTGCTGCT TTTCATGCAG CTTCTAGAGC CATATCTGGT 
GGTCCAATTT ACATAAGTGA CACTGTTGGA AATCACAATT TTGACCTTCT CAAGAAACTA 
GCTTTGCCTG ATGGTACCAT CCTTAGATGT GAAACCTATG CTCTCCCAAC TAAGGACTGT 
CTCTTTTCTG ATCCTTTACA TGATGGCAAA ACTATGCTCA AAATTTGGAA CCTCAATAAG 
TACACTGGAG TTCTTGGTGT GTTTAACTGC CAAGGAGGAG GATGGTTTCG CGAGATAAGA 
TCAAACAAAT GTGCTTCTGA GTTTTCACAT TCGGTATCAA CTAAGATCAA CATCAAAGAC 
ATTGAATGGA ACAGTGGAAA GAATCCAATT CCCATTGAAG GAGTTCAAGT TTTTGCTTTG 
TATTTCAGCC AAGCCAAGAA GCTGAAACTT TTCTCTCCAT CTGAAATTGA AGAAATCTCT 
TTGGAGCCAT TCAACTTTGA GCTTATAACT GTTTCTCCAG TCACTTTATT TCCTAAAAAG 
TCCTTGAAGT TTGCTCCTAT TGGTTTGGTT AACATGCTAA ACAATGGTGG GGCAATTCAG 
TCATTTGAAT ATCTTGAGGC TCAAGATTTG GTGCAAGTTG GAATTAGAGG TGCTGGTGAG 
ATGAGGGTCT ATGCTTCAGA GAAACCAAGG GCTTGTGGAA TTGATGGCAA AGAAGTTGAT 
TTTGAATATA AAGAGTTCAT GGTTGTGATT CAAGTACCAT GGCCTAGTTC TTCAAAATGG 
TCCTTTGTTC AGTATATATT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
BMC genomics15312(2014 Apr)
Tang H,Krishnakumar V,Bidwell S,Rosen B,Chan A,Zhou S,Gentzbittel L,Childs KL,Yandell M,Gundlach H,Mayer KF,Schwartz DC,Town CD

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Nature480(7378)520-4(2011 Nov)
Young ND,Debell? F,Oldroyd GE,Geurts R,Cannon SB,Udvardi MK,Benedito VA,Mayer KF,Gouzy J,Schoof H,Van de Peer Y,Proost S,Cook DR,Meyers BC,Spannagl M,Cheung F,De Mita S,Krishnakumar V,Gundlach H,Zhou S,Mudge J,Bharti AK,Murray JD,Naoumkina MA,Rosen B,Silverstein KA,Tang H,Rombauts S,Zhao PX,Zhou P,Barbe V,Bardou P,Bechner M,Bellec A,Berger A,Berg?s H,Bidwell S,Bisseling T,Choisne N,Couloux A,Denny R,Deshpande S,Dai X,Doyle JJ,Dudez AM,Farmer AD,Fouteau S,Franken C,Gibelin C,Gish J,Goldstein S,Gonz?lez AJ,Green PJ,Hallab A,Hartog M,Hua A,Humphray SJ,Jeong DH,Jing Y,J?cker A,Kenton SM,Kim DJ,Klee K,Lai H,Lang C,Lin S,Macmil SL,Magdelenat G,Matthews L,McCorrison J,Monaghan EL,Mun JH,Najar FZ,Nicholson C,Noirot C,O'Bleness M,Paule CR,Poulain J,Prion F,Qin B,Qu C,Retzel EF,Riddle C,Sallet E,Samain S,Samson N,Sanders I,Saurat O,Scarpelli C,Schiex T,Segurens B,Severin AJ,Sherrier DJ,Shi R,Sims S,Singer SR,Sinharoy S,Sterck L,Viollet A,Wang BB,Wang K,Wang M,Wang X,Warfsmann J,Weissenbach J,White DD,White JD,Wiley GB,Wincker P,Xing Y,Yang L,Yao Z,Ying F,Zhai J,Zhou L,Zuber A,D?nari? J,Dixon RA,May GD,Schwartz DC,Rogers J,Qu?tier F,Town CD,Roe BA

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MTR_6g004880 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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