MTR_4g119413 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene
Clones
gRNAs

Summary

Gene Symbol	MTR_4g119413
Entrez Gene ID	25494069
Full Name	cullin 3B
Gene Type	protein-coding
Organism	Medicago truncatula(barrel medic)

mRNA and Protein(s)

mRNA	Protein	Name
XM_013602795.1	XP_013458249.1	cullin 3B
XM_013602796.2	XP_013458250.1	cullin-1
XM_013602796.1	XP_013458250.1	cullin 3B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
Tang H, Krishnakumar V, Bidwell S, Rosen B, Chan A, Zhou S, Gentzbittel L, Childs KL, Yandell M, Gundlach H, Mayer KF, Schwartz DC, Town CD
BMC genomics15312(2014 Apr)

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Young ND, Debell? F, Oldroyd GE, Geurts R, Cannon SB, Udvardi MK, Benedito VA, Mayer KF, Gouzy J, Schoof H, Van de Peer Y, Proost S, Cook DR, Meyers BC, Spannagl M, Cheung F, De Mita S, Krishnakumar V, Gundlach H, Zhou S, Mudge J, Bharti AK, Murray JD, Naoumkina MA, Rosen B, Silverstein KA, Tang H, Rombauts S, Zhao PX, Zhou P, Barbe V, Bardou P, Bechner M, Bellec A, Berger A, Berg?s H, Bidwell S, Bisseling T, Choisne N, Couloux A, Denny R, Deshpande S, Dai X, Doyle JJ, Dudez AM, Farmer AD, Fouteau S, Franken C, Gibelin C, Gish J, Goldstein S, Gonz?lez AJ, Green PJ, Hallab A, Hartog M, Hua A, Humphray SJ, Jeong DH, Jing Y, J?cker A, Kenton SM, Kim DJ, Klee K, Lai H, Lang C, Lin S, Macmil SL, Magdelenat G, Matthews L, McCorrison J, Monaghan EL, Mun JH, Najar FZ, Nicholson C, Noirot C, O'Bleness M, Paule CR, Poulain J, Prion F, Qin B, Qu C, Retzel EF, Riddle C, Sallet E, Samain S, Samson N, Sanders I, Saurat O, Scarpelli C, Schiex T, Segurens B, Severin AJ, Sherrier DJ, Shi R, Sims S, Singer SR, Sinharoy S, Sterck L, Viollet A, Wang BB, Wang K, Wang M, Wang X, Warfsmann J, Weissenbach J, White DD, White JD, Wiley GB, Wincker P, Xing Y, Yang L, Yao Z, Ying F, Zhai J, Zhou L, Zuber A, D?nari? J, Dixon RA, May GD, Schwartz DC, Rogers J, Qu?tier F, Town CD, Roe BA
Nature480(7378)520-4(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MTR_4g119413 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MTR_4g119413 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe190357	XM_013602795.1 Latest version!	Medicago truncatula cullin 3B partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OMe190358	XM_013602796.2 Latest version!	Medicago truncatula cullin-1 (LOC25494069), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OMe190358	XM_013602796.1	Medicago truncatula cullin 3B mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MTR_4g119413 ( XM_013602795.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_013458249.1 Medicago truncatula cullin 3B partial mRNA.

Price & Availability↑

CloneID	OMe190357
Clone ID Related Accession (Same CDS sequence)	XM_013602795.1
Accession Version	XM_013602795.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-24
Organism	Medicago truncatula(barrel medic)
Product	cullin 3B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_016410). ##Genome-Annotation-Data-START## Annotation Provider :: JCVI Annotation Version :: Mt4.0v2 Annotation Pipeline :: JCVI Eukaryotic Genome Annotation Pipeline Annotation Method :: EVidenceModeler (EVM), PASA Features Annotated :: Gene; mRNA; CDS; tRNA URL :: http://www.jcvi.org/medicago ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on the 5' end.

ATGTCAATGA GTGAAAGGAA AACTATAGAC CTAGAACAAG GATGGGATTT TATGCATAGG 
GGTATTATGA AGCTCAAGAA CATTCTTGAA GGTTTGCCTG AACCTCAATT CAGTCCTGAA 
GATTATATGA TGCTGTACAC AACTATATAC AATATGTGTA CTCAAAAGCC TCCTAATGAC 
TATTCTCAGC CATTGTATGA CAAGTACAAG GAAGCGTTCG AAGAGTACAT CGTGTCAACG 
GTCTTACCTT CTTTGAGAGA AAAACATGAT GAGTTTATGT TACGAGAACT TGTAAAGAGA 
TGGGCGAACC ATAAAATCAT GGTGAGGTGG CTTTCTCGAT TCTTTCACTA TTTGGACCGC 
TACTTCATTG CCCGGAGGTC ACTTCCACCT CTTAATGAAG TTGGGTTGGC TTGCTTCCGT 
GATTTGGTTT ACAAGGAACT GCATGGGAAA ATGAGAGATG CAATTATTTC ACTAATTGAT 
CAAGAACGTG AGGGAGAGCA GATTGATCGA GCTCTATTAA AGAATGTATT AGATATATTT 
GTTGAAATTG GAATGGGGAA AATGGACCAC TATGAGAATG ATTTTGAAGC TGACATGCTC 
AAAGACACTT CTGCTTATTA TTCTCGAAAG GCTTCAAATT GGATCCTAGA AGATTCTTGT 
CCTGATTATA TGCTCAAGGC TGAGGAGTGC TTAAGACGGG AGAAAGATAG GGTGGCTCAT 
TACCTGCACT CTAGTAGTGA GCCAAAGTTA TTAGAGAAAG TTCAAAATGA GCTGTTGTCT 
GTATATGCAA GTCAGCTCCT TGAGAAAGAG CACTCGGGTT GTCATGCTCT ACTTAGAGAT 
GATAAGTGTG AAGACTTGTC AAGGATGTTC AGGCTATTTT CTAAAATACC CCGTGGCTTG 
GATCCTGTTT CTAGTATATT TAAGCAGCAT GTTACCACTG AAGGCATGGC GTTGGTGAAG 
CATGCTGAAG ATGCAGCTAG TAACAAAAAG GCAGAAAAAA GGGATATTGT TGGTACGCAG 
GAGCAGGTTT TTGTCCGGAA AGTGATTGAG CTACATGACA AGTACCTGGC ATATGTGAAC 
TCCTGTTTCC AGAATCACAC TCTTTTCCAC AAGGCTCTTA AAGAGGCTTT TGAGGTCTTT 
TGCAACAAAG GTGTTGCTGG AAACTCTAGT GCAGAACTTC TTGCCACTTT TTGTGATAAC 
ATTCTTAAGA AAGGAGGAAG TGAAAAATTG AGTGATGAAG CAATTGAAGA AACTCTTGAA 
AAGGTGGTAA AGCTGCTCGC TTATATTAGT GACAAGGACT TGTTTGCCGA GTTCTACAGG 
AAGAAGCTTG CTCGCAGGCT TCTTTTTGAC AAGAGTGCCA ATGATGATCA CGAGAGAAGT 
ATTTTGACAA AATTGAAGCA ACAGTGTGGT GGACAGTTTA CCTCAAAGAT GGAAGGAATG 
GTTACAGATT TGACGCTGGC AAAGGAGAAT CAAACTAGCT TTGAGGAGTA TTTAAGCAAT 
ACTCCTAATG CAGACCCTGG AATAGACTTA ACAGTTACAG TTCTGACCAC TGGTTTCTGG 
CCTAGTTATA AATCTTTTGA TCTCAATCTT CCTGCGGAAA TGGTTAAGTG CGTTGAAGTT 
TTCAAAGAAT TTTATTCAAC TAAAACAAAG CACAGAAAAC TTACATGGAT TTACTCCTTG 
GGTACCTGTA ACATAAGTGG GAAATTTGAC CCAAAAACCG TGGAGCTTGT TGTCACAACC 
TACCAGGTTA TATCACTTAC CACCTGCTTA TATGTGATAA TGTATTTGTT ATGCCCTCAC 
CCGTTCATTT TACCATTTTG TGATGTCCGC AAATTTTCAT ATGGATTTTT TTTCAACAGG 
CCTCAGCATT GTTGCTTTTT AATTCCTCTG ATAGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013458249.1
CDS	1..1899
Translation	MSMSERKTIDLEQGWDFMHRGIMKLKNILEGLPEPQFSPEDYMMLYTTIYNMCTQKPPNDYSQPLYDKYKEAFEEYIVSTVLPSLREKHDEFMLRELVKRWANHKIMVRWLSRFFHYLDRYFIARRSLPPLNEVGLACFRDLVYKELHGKMRDAIISLIDQEREGEQIDRALLKNVLDIFVEIGMGKMDHYENDFEADMLKDTSAYYSRKASNWILEDSCPDYMLKAEECLRREKDRVAHYLHSSSEPKLLEKVQNELLSVYASQLLEKEHSGCHALLRDDKCEDLSRMFRLFSKIPRGLDPVSSIFKQHVTTEGMALVKHAEDAASNKKAEKRDIVGTQEQVFVRKVIELHDKYLAYVNSCFQNHTLFHKALKEAFEVFCNKGVAGNSSAELLATFCDNILKKGGSEKLSDEAIEETLEKVVKLLAYISDKDLFAEFYRKKLARRLLFDKSANDDHERSILTKLKQQCGGQFTSKMEGMVTDLTLAKENQTSFEEYLSNTPNADPGIDLTVTVLTTGFWPSYKSFDLNLPAEMVKCVEVFKEFYSTKTKHRKLTWIYSLGTCNISGKFDPKTVELVVTTYQVISLTTCLYVIMYLLCPHPFILPFCDVRKFSYGFFFNRPQHCCFLIPLID

Target ORF information:

RefSeq Version	XM_013602795.1
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula cullin 3B partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013602795.1

ORF Insert Sequence:

ATGTCAATGA GTGAAAGGAA AACTATAGAC CTAGAACAAG GATGGGATTT TATGCATAGG 
GGTATTATGA AGCTCAAGAA CATTCTTGAA GGTTTGCCTG AACCTCAATT CAGTCCTGAA 
GATTATATGA TGCTGTACAC AACTATATAC AATATGTGTA CTCAAAAGCC TCCTAATGAC 
TATTCTCAGC CATTGTATGA CAAGTACAAG GAAGCGTTCG AAGAGTACAT CGTGTCAACG 
GTCTTACCTT CTTTGAGAGA AAAACATGAT GAGTTTATGT TACGAGAACT TGTAAAGAGA 
TGGGCGAACC ATAAAATCAT GGTGAGGTGG CTTTCTCGAT TCTTTCACTA TTTGGACCGC 
TACTTCATTG CCCGGAGGTC ACTTCCACCT CTTAATGAAG TTGGGTTGGC TTGCTTCCGT 
GATTTGGTTT ACAAGGAACT GCATGGGAAA ATGAGAGATG CAATTATTTC ACTAATTGAT 
CAAGAACGTG AGGGAGAGCA GATTGATCGA GCTCTATTAA AGAATGTATT AGATATATTT 
GTTGAAATTG GAATGGGGAA AATGGACCAC TATGAGAATG ATTTTGAAGC TGACATGCTC 
AAAGACACTT CTGCTTATTA TTCTCGAAAG GCTTCAAATT GGATCCTAGA AGATTCTTGT 
CCTGATTATA TGCTCAAGGC TGAGGAGTGC TTAAGACGGG AGAAAGATAG GGTGGCTCAT 
TACCTGCACT CTAGTAGTGA GCCAAAGTTA TTAGAGAAAG TTCAAAATGA GCTGTTGTCT 
GTATATGCAA GTCAGCTCCT TGAGAAAGAG CACTCGGGTT GTCATGCTCT ACTTAGAGAT 
GATAAGTGTG AAGACTTGTC AAGGATGTTC AGGCTATTTT CTAAAATACC CCGTGGCTTG 
GATCCTGTTT CTAGTATATT TAAGCAGCAT GTTACCACTG AAGGCATGGC GTTGGTGAAG 
CATGCTGAAG ATGCAGCTAG TAACAAAAAG GCAGAAAAAA GGGATATTGT TGGTACGCAG 
GAGCAGGTTT TTGTCCGGAA AGTGATTGAG CTACATGACA AGTACCTGGC ATATGTGAAC 
TCCTGTTTCC AGAATCACAC TCTTTTCCAC AAGGCTCTTA AAGAGGCTTT TGAGGTCTTT 
TGCAACAAAG GTGTTGCTGG AAACTCTAGT GCAGAACTTC TTGCCACTTT TTGTGATAAC 
ATTCTTAAGA AAGGAGGAAG TGAAAAATTG AGTGATGAAG CAATTGAAGA AACTCTTGAA 
AAGGTGGTAA AGCTGCTCGC TTATATTAGT GACAAGGACT TGTTTGCCGA GTTCTACAGG 
AAGAAGCTTG CTCGCAGGCT TCTTTTTGAC AAGAGTGCCA ATGATGATCA CGAGAGAAGT 
ATTTTGACAA AATTGAAGCA ACAGTGTGGT GGACAGTTTA CCTCAAAGAT GGAAGGAATG 
GTTACAGATT TGACGCTGGC AAAGGAGAAT CAAACTAGCT TTGAGGAGTA TTTAAGCAAT 
ACTCCTAATG CAGACCCTGG AATAGACTTA ACAGTTACAG TTCTGACCAC TGGTTTCTGG 
CCTAGTTATA AATCTTTTGA TCTCAATCTT CCTGCGGAAA TGGTTAAGTG CGTTGAAGTT 
TTCAAAGAAT TTTATTCAAC TAAAACAAAG CACAGAAAAC TTACATGGAT TTACTCCTTG 
GGTACCTGTA ACATAAGTGG GAAATTTGAC CCAAAAACCG TGGAGCTTGT TGTCACAACC 
TACCAGGTTA TATCACTTAC CACCTGCTTA TATGTGATAA TGTATTTGTT ATGCCCTCAC 
CCGTTCATTT TACCATTTTG TGATGTCCGC AAATTTTCAT ATGGATTTTT TTTCAACAGG 
CCTCAGCATT GTTGCTTTTT AATTCCTCTG ATAGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTR_4g119413 ( XM_013602796.2 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_013458250.1 Medicago truncatula cullin-1 (LOC25494069), mRNA.

Price & Availability↑

CloneID	OMe190358
Clone ID Related Accession (Same CDS sequence)	XM_013602796.2 , XM_013602796.1
Accession Version	XM_013602796.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	cullin-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016410.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2018 this sequence version replaced XM_013602796.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAATGA GTGAAAGGAA AACTATAGAC CTAGAACAAG GATGGGATTT TATGCATAGG 
GGTATTATGA AGCTCAAGAA CATTCTTGAA GGTTTGCCTG AACCTCAATT CAGTCCTGAA 
GATTATATGA TGCTGTACAC AACTATATAC AATATGTGTA CTCAAAAGCC TCCTAATGAC 
TATTCTCAGC CATTGTATGA CAAGTACAAG GAAGCGTTCG AAGAGTACAT CGTGTCAACG 
GTCTTACCTT CTTTGAGAGA AAAACATGAT GAGTTTATGT TACGAGAACT TGTAAAGAGA 
TGGGCGAACC ATAAAATCAT GGTGAGGTGG CTTTCTCGAT TCTTTCACTA TTTGGACCGC 
TACTTCATTG CCCGGAGGTC ACTTCCACCT CTTAATGAAG TTGGGTTGGC TTGCTTCCGT 
GATTTGGTTT ACAAGGAACT GCATGGGAAA ATGAGAGATG CAATTATTTC ACTAATTGAT 
CAAGAACGTG AGGGAGAGCA GATTGATCGA GCTCTATTAA AGAATGTATT AGATATATTT 
GTTGAAATTG GAATGGGGAA AATGGACCAC TATGAGAATG ATTTTGAAGC TGACATGCTC 
AAAGACACTT CTGCTTATTA TTCTCGAAAG GCTTCAAATT GGATCCTAGA AGATTCTTGT 
CCTGATTATA TGCTCAAGGC TGAGGAGTGC TTAAGACGGG AGAAAGATAG GGTGGCTCAT 
TACCTGCACT CTAGTAGTGA GCCAAAGTTA TTAGAGAAAG TTCAAAATGA GCTGTTGTCT 
GTATATGCAA GTCAGCTCCT TGAGAAAGAG CACTCGGGTT GTCATGCTCT ACTTAGAGAT 
GATAAGTGTG AAGACTTGTC AAGGATGTTC AGGCTATTTT CTAAAATACC CCGTGGCTTG 
GATCCTGTTT CTAGTATATT TAAGCAGCAT GTTACCACTG AAGGCATGGC GTTGGTGAAG 
CATGCTGAAG ATGCAGCTAG TAACAAAAAG GCAGAAAAAA GGGATATTGT TGGTACGCAG 
GAGCAGGTTT TTGTCCGGAA AGTGATTGAG CTACATGACA AGTACCTGGC ATATGTGAAC 
TCCTGTTTCC AGAATCACAC TCTTTTCCAC AAGGCTCTTA AAGAGGCTTT TGAGGTCTTT 
TGCAACAAAG GTGTTGCTGG AAACTCTAGT GCAGAACTTC TTGCCACTTT TTGTGATAAC 
ATTCTTAAGA AAGGAGGAAG TGAAAAATTG AGTGATGAAG CAATTGAAGA AACTCTTGAA 
AAGGTGGTAA AGCTGCTCGC TTATATTAGT GACAAGGACT TGTTTGCCGA GTTCTACAGG 
AAGAAGCTTG CTCGCAGGCT TCTTTTTGAC AAGAGTGCCA ATGATGATCA CGAGAGAAGT 
ATTTTGACAA AATTGAAGCA ACAGTGTGGT GGACAGTTTA CCTCAAAGAT GGAAGGAATG 
GTTACAGATT TGACGCTGGC AAAGGAGAAT CAAACTAGCT TTGAGGAGTA TTTAAGCAAT 
ACTCCTAATG CAGACCCTGG AATAGACTTA ACAGTTACAG TTCTGACCAC TGGTTTCTGG 
CCTAGTTATA AATCTTTTGA TCTCAATCTT CCTGCGGAAA TGGTTAAGTG CGTTGAAGTT 
TTCAAAGAAT TTTATTCAAC TAAAACAAAG CACAGAAAAC TTACATGGAT TTACTCCTTG 
GGTACCTGTA ACATAAGTGG GAAATTTGAC CCAAAAACCG TGGAGCTTGT TGTCACAACC 
TACCAGGCCT CAGCATTGTT GCTTTTTAAT TCCTCTGATA GATTGAGTTA TTCTGAGATA 
ATGACTCAGT TAAACTTATT GGATGAAGAT GTCATTAGGC TACTCCACTC CTTGTCATGT 
GCAAAGTATA AGATTCTTAT CAAGGAGCCA AACACAAAAA CGATATTACC CACTGATTAT 
TTTGAATTTA ATGCAAAGTT CACTGACAAA ATGAGGAGGA TCAAGATTCC TCTACCTCCT 
GTGGATGAGA AGAAAAAAGT AATTGAGGAT GTTGACAAGG ACAGAAGATA TGCCATTGAT 
GCCTCAATTG TACGCATTAT GAAGAGTCGG AAAGTTTTGG GCTATCAACA GTTAGTTATG 
GAGTGCGTTG AGCAGTTGGG TCGCATGTTT AAGCCTGATG TCAAAGCAAT TAAAAAGCGG 
ATTGAAGATT TGATTTCTCG GGATTACTTG GAAAGAGACA AAGAGAATCC AAATATGTTC 
AAGTACTTAG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013458250.1
CDS	216..2450
Translation	MSMSERKTIDLEQGWDFMHRGIMKLKNILEGLPEPQFSPEDYMMLYTTIYNMCTQKPPNDYSQPLYDKYKEAFEEYIVSTVLPSLREKHDEFMLRELVKRWANHKIMVRWLSRFFHYLDRYFIARRSLPPLNEVGLACFRDLVYKELHGKMRDAIISLIDQEREGEQIDRALLKNVLDIFVEIGMGKMDHYENDFEADMLKDTSAYYSRKASNWILEDSCPDYMLKAEECLRREKDRVAHYLHSSSEPKLLEKVQNELLSVYASQLLEKEHSGCHALLRDDKCEDLSRMFRLFSKIPRGLDPVSSIFKQHVTTEGMALVKHAEDAASNKKAEKRDIVGTQEQVFVRKVIELHDKYLAYVNSCFQNHTLFHKALKEAFEVFCNKGVAGNSSAELLATFCDNILKKGGSEKLSDEAIEETLEKVVKLLAYISDKDLFAEFYRKKLARRLLFDKSANDDHERSILTKLKQQCGGQFTSKMEGMVTDLTLAKENQTSFEEYLSNTPNADPGIDLTVTVLTTGFWPSYKSFDLNLPAEMVKCVEVFKEFYSTKTKHRKLTWIYSLGTCNISGKFDPKTVELVVTTYQASALLLFNSSDRLSYSEIMTQLNLLDEDVIRLLHSLSCAKYKILIKEPNTKTILPTDYFEFNAKFTDKMRRIKIPLPPVDEKKKVIEDVDKDRRYAIDASIVRIMKSRKVLGYQQLVMECVEQLGRMFKPDVKAIKKRIEDLISRDYLERDKENPNMFKYLA

Target ORF information:

RefSeq Version	XM_013602796.2
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula cullin-1 (LOC25494069), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013602796.2

ORF Insert Sequence:

ATGTCAATGA GTGAAAGGAA AACTATAGAC CTAGAACAAG GATGGGATTT TATGCATAGG 
GGTATTATGA AGCTCAAGAA CATTCTTGAA GGTTTGCCTG AACCTCAATT CAGTCCTGAA 
GATTATATGA TGCTGTACAC AACTATATAC AATATGTGTA CTCAAAAGCC TCCTAATGAC 
TATTCTCAGC CATTGTATGA CAAGTACAAG GAAGCGTTCG AAGAGTACAT CGTGTCAACG 
GTCTTACCTT CTTTGAGAGA AAAACATGAT GAGTTTATGT TACGAGAACT TGTAAAGAGA 
TGGGCGAACC ATAAAATCAT GGTGAGGTGG CTTTCTCGAT TCTTTCACTA TTTGGACCGC 
TACTTCATTG CCCGGAGGTC ACTTCCACCT CTTAATGAAG TTGGGTTGGC TTGCTTCCGT 
GATTTGGTTT ACAAGGAACT GCATGGGAAA ATGAGAGATG CAATTATTTC ACTAATTGAT 
CAAGAACGTG AGGGAGAGCA GATTGATCGA GCTCTATTAA AGAATGTATT AGATATATTT 
GTTGAAATTG GAATGGGGAA AATGGACCAC TATGAGAATG ATTTTGAAGC TGACATGCTC 
AAAGACACTT CTGCTTATTA TTCTCGAAAG GCTTCAAATT GGATCCTAGA AGATTCTTGT 
CCTGATTATA TGCTCAAGGC TGAGGAGTGC TTAAGACGGG AGAAAGATAG GGTGGCTCAT 
TACCTGCACT CTAGTAGTGA GCCAAAGTTA TTAGAGAAAG TTCAAAATGA GCTGTTGTCT 
GTATATGCAA GTCAGCTCCT TGAGAAAGAG CACTCGGGTT GTCATGCTCT ACTTAGAGAT 
GATAAGTGTG AAGACTTGTC AAGGATGTTC AGGCTATTTT CTAAAATACC CCGTGGCTTG 
GATCCTGTTT CTAGTATATT TAAGCAGCAT GTTACCACTG AAGGCATGGC GTTGGTGAAG 
CATGCTGAAG ATGCAGCTAG TAACAAAAAG GCAGAAAAAA GGGATATTGT TGGTACGCAG 
GAGCAGGTTT TTGTCCGGAA AGTGATTGAG CTACATGACA AGTACCTGGC ATATGTGAAC 
TCCTGTTTCC AGAATCACAC TCTTTTCCAC AAGGCTCTTA AAGAGGCTTT TGAGGTCTTT 
TGCAACAAAG GTGTTGCTGG AAACTCTAGT GCAGAACTTC TTGCCACTTT TTGTGATAAC 
ATTCTTAAGA AAGGAGGAAG TGAAAAATTG AGTGATGAAG CAATTGAAGA AACTCTTGAA 
AAGGTGGTAA AGCTGCTCGC TTATATTAGT GACAAGGACT TGTTTGCCGA GTTCTACAGG 
AAGAAGCTTG CTCGCAGGCT TCTTTTTGAC AAGAGTGCCA ATGATGATCA CGAGAGAAGT 
ATTTTGACAA AATTGAAGCA ACAGTGTGGT GGACAGTTTA CCTCAAAGAT GGAAGGAATG 
GTTACAGATT TGACGCTGGC AAAGGAGAAT CAAACTAGCT TTGAGGAGTA TTTAAGCAAT 
ACTCCTAATG CAGACCCTGG AATAGACTTA ACAGTTACAG TTCTGACCAC TGGTTTCTGG 
CCTAGTTATA AATCTTTTGA TCTCAATCTT CCTGCGGAAA TGGTTAAGTG CGTTGAAGTT 
TTCAAAGAAT TTTATTCAAC TAAAACAAAG CACAGAAAAC TTACATGGAT TTACTCCTTG 
GGTACCTGTA ACATAAGTGG GAAATTTGAC CCAAAAACCG TGGAGCTTGT TGTCACAACC 
TACCAGGCCT CAGCATTGTT GCTTTTTAAT TCCTCTGATA GATTGAGTTA TTCTGAGATA 
ATGACTCAGT TAAACTTATT GGATGAAGAT GTCATTAGGC TACTCCACTC CTTGTCATGT 
GCAAAGTATA AGATTCTTAT CAAGGAGCCA AACACAAAAA CGATATTACC CACTGATTAT 
TTTGAATTTA ATGCAAAGTT CACTGACAAA ATGAGGAGGA TCAAGATTCC TCTACCTCCT 
GTGGATGAGA AGAAAAAAGT AATTGAGGAT GTTGACAAGG ACAGAAGATA TGCCATTGAT 
GCCTCAATTG TACGCATTAT GAAGAGTCGG AAAGTTTTGG GCTATCAACA GTTAGTTATG 
GAGTGCGTTG AGCAGTTGGG TCGCATGTTT AAGCCTGATG TCAAAGCAAT TAAAAAGCGG 
ATTGAAGATT TGATTTCTCG GGATTACTTG GAAAGAGACA AAGAGAATCC AAATATGTTC 
AAGTACTTAG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTR_4g119413 ( XM_013602796.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_013458250.1 Medicago truncatula cullin 3B mRNA.

Price & Availability↑

CloneID	OMe190358
Clone ID Related Accession (Same CDS sequence)	XM_013602796.2 , XM_013602796.1
Accession Version	XM_013602796.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-24
Organism	Medicago truncatula(barrel medic)
Product	cullin 3B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_016410). ##Genome-Annotation-Data-START## Annotation Provider :: JCVI Annotation Version :: Mt4.0v2 Annotation Pipeline :: JCVI Eukaryotic Genome Annotation Pipeline Annotation Method :: EVidenceModeler (EVM), PASA Features Annotated :: Gene; mRNA; CDS; tRNA URL :: http://www.jcvi.org/medicago ##Genome-Annotation-Data-END##

ATGTCAATGA GTGAAAGGAA AACTATAGAC CTAGAACAAG GATGGGATTT TATGCATAGG 
GGTATTATGA AGCTCAAGAA CATTCTTGAA GGTTTGCCTG AACCTCAATT CAGTCCTGAA 
GATTATATGA TGCTGTACAC AACTATATAC AATATGTGTA CTCAAAAGCC TCCTAATGAC 
TATTCTCAGC CATTGTATGA CAAGTACAAG GAAGCGTTCG AAGAGTACAT CGTGTCAACG 
GTCTTACCTT CTTTGAGAGA AAAACATGAT GAGTTTATGT TACGAGAACT TGTAAAGAGA 
TGGGCGAACC ATAAAATCAT GGTGAGGTGG CTTTCTCGAT TCTTTCACTA TTTGGACCGC 
TACTTCATTG CCCGGAGGTC ACTTCCACCT CTTAATGAAG TTGGGTTGGC TTGCTTCCGT 
GATTTGGTTT ACAAGGAACT GCATGGGAAA ATGAGAGATG CAATTATTTC ACTAATTGAT 
CAAGAACGTG AGGGAGAGCA GATTGATCGA GCTCTATTAA AGAATGTATT AGATATATTT 
GTTGAAATTG GAATGGGGAA AATGGACCAC TATGAGAATG ATTTTGAAGC TGACATGCTC 
AAAGACACTT CTGCTTATTA TTCTCGAAAG GCTTCAAATT GGATCCTAGA AGATTCTTGT 
CCTGATTATA TGCTCAAGGC TGAGGAGTGC TTAAGACGGG AGAAAGATAG GGTGGCTCAT 
TACCTGCACT CTAGTAGTGA GCCAAAGTTA TTAGAGAAAG TTCAAAATGA GCTGTTGTCT 
GTATATGCAA GTCAGCTCCT TGAGAAAGAG CACTCGGGTT GTCATGCTCT ACTTAGAGAT 
GATAAGTGTG AAGACTTGTC AAGGATGTTC AGGCTATTTT CTAAAATACC CCGTGGCTTG 
GATCCTGTTT CTAGTATATT TAAGCAGCAT GTTACCACTG AAGGCATGGC GTTGGTGAAG 
CATGCTGAAG ATGCAGCTAG TAACAAAAAG GCAGAAAAAA GGGATATTGT TGGTACGCAG 
GAGCAGGTTT TTGTCCGGAA AGTGATTGAG CTACATGACA AGTACCTGGC ATATGTGAAC 
TCCTGTTTCC AGAATCACAC TCTTTTCCAC AAGGCTCTTA AAGAGGCTTT TGAGGTCTTT 
TGCAACAAAG GTGTTGCTGG AAACTCTAGT GCAGAACTTC TTGCCACTTT TTGTGATAAC 
ATTCTTAAGA AAGGAGGAAG TGAAAAATTG AGTGATGAAG CAATTGAAGA AACTCTTGAA 
AAGGTGGTAA AGCTGCTCGC TTATATTAGT GACAAGGACT TGTTTGCCGA GTTCTACAGG 
AAGAAGCTTG CTCGCAGGCT TCTTTTTGAC AAGAGTGCCA ATGATGATCA CGAGAGAAGT 
ATTTTGACAA AATTGAAGCA ACAGTGTGGT GGACAGTTTA CCTCAAAGAT GGAAGGAATG 
GTTACAGATT TGACGCTGGC AAAGGAGAAT CAAACTAGCT TTGAGGAGTA TTTAAGCAAT 
ACTCCTAATG CAGACCCTGG AATAGACTTA ACAGTTACAG TTCTGACCAC TGGTTTCTGG 
CCTAGTTATA AATCTTTTGA TCTCAATCTT CCTGCGGAAA TGGTTAAGTG CGTTGAAGTT 
TTCAAAGAAT TTTATTCAAC TAAAACAAAG CACAGAAAAC TTACATGGAT TTACTCCTTG 
GGTACCTGTA ACATAAGTGG GAAATTTGAC CCAAAAACCG TGGAGCTTGT TGTCACAACC 
TACCAGGCCT CAGCATTGTT GCTTTTTAAT TCCTCTGATA GATTGAGTTA TTCTGAGATA 
ATGACTCAGT TAAACTTATT GGATGAAGAT GTCATTAGGC TACTCCACTC CTTGTCATGT 
GCAAAGTATA AGATTCTTAT CAAGGAGCCA AACACAAAAA CGATATTACC CACTGATTAT 
TTTGAATTTA ATGCAAAGTT CACTGACAAA ATGAGGAGGA TCAAGATTCC TCTACCTCCT 
GTGGATGAGA AGAAAAAAGT AATTGAGGAT GTTGACAAGG ACAGAAGATA TGCCATTGAT 
GCCTCAATTG TACGCATTAT GAAGAGTCGG AAAGTTTTGG GCTATCAACA GTTAGTTATG 
GAGTGCGTTG AGCAGTTGGG TCGCATGTTT AAGCCTGATG TCAAAGCAAT TAAAAAGCGG 
ATTGAAGATT TGATTTCTCG GGATTACTTG GAAAGAGACA AAGAGAATCC AAATATGTTC 
AAGTACTTAG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013458250.1
CDS	294..2528
Translation	MSMSERKTIDLEQGWDFMHRGIMKLKNILEGLPEPQFSPEDYMMLYTTIYNMCTQKPPNDYSQPLYDKYKEAFEEYIVSTVLPSLREKHDEFMLRELVKRWANHKIMVRWLSRFFHYLDRYFIARRSLPPLNEVGLACFRDLVYKELHGKMRDAIISLIDQEREGEQIDRALLKNVLDIFVEIGMGKMDHYENDFEADMLKDTSAYYSRKASNWILEDSCPDYMLKAEECLRREKDRVAHYLHSSSEPKLLEKVQNELLSVYASQLLEKEHSGCHALLRDDKCEDLSRMFRLFSKIPRGLDPVSSIFKQHVTTEGMALVKHAEDAASNKKAEKRDIVGTQEQVFVRKVIELHDKYLAYVNSCFQNHTLFHKALKEAFEVFCNKGVAGNSSAELLATFCDNILKKGGSEKLSDEAIEETLEKVVKLLAYISDKDLFAEFYRKKLARRLLFDKSANDDHERSILTKLKQQCGGQFTSKMEGMVTDLTLAKENQTSFEEYLSNTPNADPGIDLTVTVLTTGFWPSYKSFDLNLPAEMVKCVEVFKEFYSTKTKHRKLTWIYSLGTCNISGKFDPKTVELVVTTYQASALLLFNSSDRLSYSEIMTQLNLLDEDVIRLLHSLSCAKYKILIKEPNTKTILPTDYFEFNAKFTDKMRRIKIPLPPVDEKKKVIEDVDKDRRYAIDASIVRIMKSRKVLGYQQLVMECVEQLGRMFKPDVKAIKKRIEDLISRDYLERDKENPNMFKYLA

Target ORF information:

RefSeq Version	XM_013602796.1
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula cullin 3B mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013602796.1

ORF Insert Sequence:

ATGTCAATGA GTGAAAGGAA AACTATAGAC CTAGAACAAG GATGGGATTT TATGCATAGG 
GGTATTATGA AGCTCAAGAA CATTCTTGAA GGTTTGCCTG AACCTCAATT CAGTCCTGAA 
GATTATATGA TGCTGTACAC AACTATATAC AATATGTGTA CTCAAAAGCC TCCTAATGAC 
TATTCTCAGC CATTGTATGA CAAGTACAAG GAAGCGTTCG AAGAGTACAT CGTGTCAACG 
GTCTTACCTT CTTTGAGAGA AAAACATGAT GAGTTTATGT TACGAGAACT TGTAAAGAGA 
TGGGCGAACC ATAAAATCAT GGTGAGGTGG CTTTCTCGAT TCTTTCACTA TTTGGACCGC 
TACTTCATTG CCCGGAGGTC ACTTCCACCT CTTAATGAAG TTGGGTTGGC TTGCTTCCGT 
GATTTGGTTT ACAAGGAACT GCATGGGAAA ATGAGAGATG CAATTATTTC ACTAATTGAT 
CAAGAACGTG AGGGAGAGCA GATTGATCGA GCTCTATTAA AGAATGTATT AGATATATTT 
GTTGAAATTG GAATGGGGAA AATGGACCAC TATGAGAATG ATTTTGAAGC TGACATGCTC 
AAAGACACTT CTGCTTATTA TTCTCGAAAG GCTTCAAATT GGATCCTAGA AGATTCTTGT 
CCTGATTATA TGCTCAAGGC TGAGGAGTGC TTAAGACGGG AGAAAGATAG GGTGGCTCAT 
TACCTGCACT CTAGTAGTGA GCCAAAGTTA TTAGAGAAAG TTCAAAATGA GCTGTTGTCT 
GTATATGCAA GTCAGCTCCT TGAGAAAGAG CACTCGGGTT GTCATGCTCT ACTTAGAGAT 
GATAAGTGTG AAGACTTGTC AAGGATGTTC AGGCTATTTT CTAAAATACC CCGTGGCTTG 
GATCCTGTTT CTAGTATATT TAAGCAGCAT GTTACCACTG AAGGCATGGC GTTGGTGAAG 
CATGCTGAAG ATGCAGCTAG TAACAAAAAG GCAGAAAAAA GGGATATTGT TGGTACGCAG 
GAGCAGGTTT TTGTCCGGAA AGTGATTGAG CTACATGACA AGTACCTGGC ATATGTGAAC 
TCCTGTTTCC AGAATCACAC TCTTTTCCAC AAGGCTCTTA AAGAGGCTTT TGAGGTCTTT 
TGCAACAAAG GTGTTGCTGG AAACTCTAGT GCAGAACTTC TTGCCACTTT TTGTGATAAC 
ATTCTTAAGA AAGGAGGAAG TGAAAAATTG AGTGATGAAG CAATTGAAGA AACTCTTGAA 
AAGGTGGTAA AGCTGCTCGC TTATATTAGT GACAAGGACT TGTTTGCCGA GTTCTACAGG 
AAGAAGCTTG CTCGCAGGCT TCTTTTTGAC AAGAGTGCCA ATGATGATCA CGAGAGAAGT 
ATTTTGACAA AATTGAAGCA ACAGTGTGGT GGACAGTTTA CCTCAAAGAT GGAAGGAATG 
GTTACAGATT TGACGCTGGC AAAGGAGAAT CAAACTAGCT TTGAGGAGTA TTTAAGCAAT 
ACTCCTAATG CAGACCCTGG AATAGACTTA ACAGTTACAG TTCTGACCAC TGGTTTCTGG 
CCTAGTTATA AATCTTTTGA TCTCAATCTT CCTGCGGAAA TGGTTAAGTG CGTTGAAGTT 
TTCAAAGAAT TTTATTCAAC TAAAACAAAG CACAGAAAAC TTACATGGAT TTACTCCTTG 
GGTACCTGTA ACATAAGTGG GAAATTTGAC CCAAAAACCG TGGAGCTTGT TGTCACAACC 
TACCAGGCCT CAGCATTGTT GCTTTTTAAT TCCTCTGATA GATTGAGTTA TTCTGAGATA 
ATGACTCAGT TAAACTTATT GGATGAAGAT GTCATTAGGC TACTCCACTC CTTGTCATGT 
GCAAAGTATA AGATTCTTAT CAAGGAGCCA AACACAAAAA CGATATTACC CACTGATTAT 
TTTGAATTTA ATGCAAAGTT CACTGACAAA ATGAGGAGGA TCAAGATTCC TCTACCTCCT 
GTGGATGAGA AGAAAAAAGT AATTGAGGAT GTTGACAAGG ACAGAAGATA TGCCATTGAT 
GCCTCAATTG TACGCATTAT GAAGAGTCGG AAAGTTTTGG GCTATCAACA GTTAGTTATG 
GAGTGCGTTG AGCAGTTGGG TCGCATGTTT AAGCCTGATG TCAAAGCAAT TAAAAAGCGG 
ATTGAAGATT TGATTTCTCG GGATTACTTG GAAAGAGACA AAGAGAATCC AAATATGTTC 
AAGTACTTAG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
BMC genomics15312(2014 Apr)
Tang H,Krishnakumar V,Bidwell S,Rosen B,Chan A,Zhou S,Gentzbittel L,Childs KL,Yandell M,Gundlach H,Mayer KF,Schwartz DC,Town CD

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Nature480(7378)520-4(2011 Nov)
Young ND,Debell? F,Oldroyd GE,Geurts R,Cannon SB,Udvardi MK,Benedito VA,Mayer KF,Gouzy J,Schoof H,Van de Peer Y,Proost S,Cook DR,Meyers BC,Spannagl M,Cheung F,De Mita S,Krishnakumar V,Gundlach H,Zhou S,Mudge J,Bharti AK,Murray JD,Naoumkina MA,Rosen B,Silverstein KA,Tang H,Rombauts S,Zhao PX,Zhou P,Barbe V,Bardou P,Bechner M,Bellec A,Berger A,Berg?s H,Bidwell S,Bisseling T,Choisne N,Couloux A,Denny R,Deshpande S,Dai X,Doyle JJ,Dudez AM,Farmer AD,Fouteau S,Franken C,Gibelin C,Gish J,Goldstein S,Gonz?lez AJ,Green PJ,Hallab A,Hartog M,Hua A,Humphray SJ,Jeong DH,Jing Y,J?cker A,Kenton SM,Kim DJ,Klee K,Lai H,Lang C,Lin S,Macmil SL,Magdelenat G,Matthews L,McCorrison J,Monaghan EL,Mun JH,Najar FZ,Nicholson C,Noirot C,O'Bleness M,Paule CR,Poulain J,Prion F,Qin B,Qu C,Retzel EF,Riddle C,Sallet E,Samain S,Samson N,Sanders I,Saurat O,Scarpelli C,Schiex T,Segurens B,Severin AJ,Sherrier DJ,Shi R,Sims S,Singer SR,Sinharoy S,Sterck L,Viollet A,Wang BB,Wang K,Wang M,Wang X,Warfsmann J,Weissenbach J,White DD,White JD,Wiley GB,Wincker P,Xing Y,Yang L,Yao Z,Ying F,Zhai J,Zhou L,Zuber A,D?nari? J,Dixon RA,May GD,Schwartz DC,Rogers J,Qu?tier F,Town CD,Roe BA

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MTR_4g119413 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

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電話

FAX

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