ORF » Species Summary » Medicago truncatula » LOC25490778 cDNA ORF clone

LOC25490778 cDNA ORF clone, Medicago truncatula(barrel medic)

The following LOC25490778 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC25490778 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMe196401 XM_013604679.1
Latest version!		 Medicago truncatula zinc finger, C3HC4 type (RING finger) protein partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00
OMe262483 XM_024780194.1
Latest version!		 Medicago truncatula probable E3 ubiquitin-protein ligase RNF217 (LOC25490778), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    LOC25490778 ( XM_013604679.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_013460133.1 Medicago truncatula zinc finger, C3HC4 type (RING finger) protein partial mRNA.
    Price & Availability↑
    CloneID OMe196401
    Clone ID Related Accession (Same CDS sequence) XM_013604679.1
    Accession Version XM_013604679.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 831bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-08-24
    Organism Medicago truncatula(barrel medic)
    Product zinc finger, C3HC4 type (RING finger) protein
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_016409). ##Genome-Annotation-Data-START## Annotation Provider :: JCVI Annotation Version :: Mt4.0v2 Annotation Pipeline :: JCVI Eukaryotic Genome Annotation Pipeline Annotation Method :: EVidenceModeler (EVM), PASA Features Annotated :: Gene; mRNA; CDS; tRNA URL :: http://www.jcvi.org/medicago ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAAGCGCT CACTCTCAGC ATGTAATAGG ATGAAGAGAA AATCTCATTC AGAAATCAAT 
    GATCAAAAGA AGAAGAAACC TGCACTCACA CCGGTTAGGA GTTCATCACA ATCATTGATC 
    TTTGATAGCA AGCCAGAAAA ATTATTCACA AAGACGATTG GTGGTGAATC CTCACGAAGC 
    TTATGTGGTA TATGCTTTGA TTTGAAAACA GATTCAGATA TGTTCAAAAG AAAGAGTTGC 
    AACCACCTCT TTTGTGTTGA ATGTATTTCC AAATATGTGG TTTCTCAAAT TAACAACAAT 
    GTGGTTAAGG TGACTTGTCC AACTCCAAAT TGTTTTGTGA AATTTGAGCC GAGACATTTG 
    AAACATATTT TGCCTAGAGA ATTTATTGAT AGATGGGAAC TTGCAGTGTA TGAGTCTAAA 
    ATTGCATTGG ACCAAAAGAC TTATTGTCCA TTTAAGAATT GTTCTGTTTT GTTGGTTAAT 
    GATAATAATA GGGGTGTGGT TTTGACTAGT TGCGAGTGTC CATCTTGTCA CCGACTATTT 
    TGTGCACAAT GTAAGGTTCC ATGGCATGCG GAGATGAATT GTCAGGAGTT TCAAGATTTG 
    AAACGTCCTA AAAAGGAACA AGATTTGGAT GATAAATTTT TGGAGTTGGC TAACAGAAAG 
    AAGTGGCAGA GATGCCCAAA TTGCTCTATC TATGTCAAGA GAAGTGATGG ATGTGTAAAT 
    GTAACTTCTG CTACCATTGT GGGGAGGAAT GGAAGCATGG GCATTTATGC GAGAAGAGAA 
    AATATTGATG ATTCCCCTAT TTTAGAGGTA ACTCTTCACA CGTTGAATTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013460133.1
    CDS26..856
    Translation

    Target ORF information:

    RefSeq Version XM_013604679.1
    Organism Medicago truncatula(barrel medic)
    Definition Medicago truncatula zinc finger, C3HC4 type (RING finger) protein partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_013604679.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAAGCGCT CACTCTCAGC ATGTAATAGG ATGAAGAGAA AATCTCATTC AGAAATCAAT 
    GATCAAAAGA AGAAGAAACC TGCACTCACA CCGGTTAGGA GTTCATCACA ATCATTGATC 
    TTTGATAGCA AGCCAGAAAA ATTATTCACA AAGACGATTG GTGGTGAATC CTCACGAAGC 
    TTATGTGGTA TATGCTTTGA TTTGAAAACA GATTCAGATA TGTTCAAAAG AAAGAGTTGC 
    AACCACCTCT TTTGTGTTGA ATGTATTTCC AAATATGTGG TTTCTCAAAT TAACAACAAT 
    GTGGTTAAGG TGACTTGTCC AACTCCAAAT TGTTTTGTGA AATTTGAGCC GAGACATTTG 
    AAACATATTT TGCCTAGAGA ATTTATTGAT AGATGGGAAC TTGCAGTGTA TGAGTCTAAA 
    ATTGCATTGG ACCAAAAGAC TTATTGTCCA TTTAAGAATT GTTCTGTTTT GTTGGTTAAT 
    GATAATAATA GGGGTGTGGT TTTGACTAGT TGCGAGTGTC CATCTTGTCA CCGACTATTT 
    TGTGCACAAT GTAAGGTTCC ATGGCATGCG GAGATGAATT GTCAGGAGTT TCAAGATTTG 
    AAACGTCCTA AAAAGGAACA AGATTTGGAT GATAAATTTT TGGAGTTGGC TAACAGAAAG 
    AAGTGGCAGA GATGCCCAAA TTGCTCTATC TATGTCAAGA GAAGTGATGG ATGTGTAAAT 
    GTAACTTCTG CTACCATTGT GGGGAGGAAT GGAAGCATGG GCATTTATGC GAGAAGAGAA 
    AATATTGATG ATTCCCCTAT TTTAGAGGTA ACTCTTCACA CGTTGAATTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LOC25490778 ( XM_024780194.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_024635962.1 Medicago truncatula probable E3 ubiquitin-protein ligase RNF217 (LOC25490778), mRNA.
    Price & Availability↑
    CloneID OMe262483
    Clone ID Related Accession (Same CDS sequence) XM_024780194.1
    Accession Version XM_024780194.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 807bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-22
    Organism Medicago truncatula(barrel medic)
    Product probable E3 ubiquitin-protein ligase RNF217
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016409.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAAGCGCT CACTCTCAGC ATGTAATAGG ATGAAGAGAA AATCTCATTC AGAAATCAAT 
    GATCAAAAGA AGAAGAAACC TGCACTCACA CCGGTTAGGA GTTCATCACA ATCATTGATC 
    TTTGATAGCA AGCCAGAAAA ATTATTCACA AAGACGATTG GTGGTGAATC CTCACGAAGC 
    TTATGTGGTA TATGCTTTGA TTTGAAAACA GATTCAGATA TGTTCAAAAG AAAGAGTTGC 
    AACCACCTCT TTTGTGTTGA ATGTATTTCC AAATATGTGG TTTCTCAAAT TAACAACAAT 
    GTGGTTAAGG TGACTTGTCC AACTCCAAAT TGTTTTGTGA AATTTGAGCC GAGACATTTG 
    AAACATATTT TGCCTAGAGA ATTTATTGAT AGATGGGAAC TTGCAGTGTA TGAGTCTAAA 
    ATTGCATTGG ACCAAAAGAC TTATTGTCCA TTTAAGAATT GTTCTGTTTT GTTGGTTAAT 
    GATAATAATA GGGGTGTGGT TTTGACTAGT TGCGAGTGTC CATCTTGTCA CCGACTATTT 
    TGTGCACAAT GTAAGGTTCC ATGGCATGCG GAGATGAATT GTCAGGAGTT TCAAGATTTG 
    AAACGTCCTA AAAAGGAACA AGATTTGGAT GATAAATTTT TGGAGTTGGC TAACAGAAAG 
    AAGTGGCAGA GATGCCCAAA TTGCTCTATC TATGTCAAGA GAAGTGATGG ATGTGAACAC 
    ATGAAATGCA GGTGTAAATG TAACTTCTGC TACCATTGTG GGGAGGAATG GAAGCATGGG 
    CATTTATGCG AGAAGAGAAA ATATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024635962.1
    CDS169..975
    Translation

    Target ORF information:

    RefSeq Version XM_024780194.1
    Organism Medicago truncatula(barrel medic)
    Definition Medicago truncatula probable E3 ubiquitin-protein ligase RNF217 (LOC25490778), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024780194.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAAGCGCT CACTCTCAGC ATGTAATAGG ATGAAGAGAA AATCTCATTC AGAAATCAAT 
    GATCAAAAGA AGAAGAAACC TGCACTCACA CCGGTTAGGA GTTCATCACA ATCATTGATC 
    TTTGATAGCA AGCCAGAAAA ATTATTCACA AAGACGATTG GTGGTGAATC CTCACGAAGC 
    TTATGTGGTA TATGCTTTGA TTTGAAAACA GATTCAGATA TGTTCAAAAG AAAGAGTTGC 
    AACCACCTCT TTTGTGTTGA ATGTATTTCC AAATATGTGG TTTCTCAAAT TAACAACAAT 
    GTGGTTAAGG TGACTTGTCC AACTCCAAAT TGTTTTGTGA AATTTGAGCC GAGACATTTG 
    AAACATATTT TGCCTAGAGA ATTTATTGAT AGATGGGAAC TTGCAGTGTA TGAGTCTAAA 
    ATTGCATTGG ACCAAAAGAC TTATTGTCCA TTTAAGAATT GTTCTGTTTT GTTGGTTAAT 
    GATAATAATA GGGGTGTGGT TTTGACTAGT TGCGAGTGTC CATCTTGTCA CCGACTATTT 
    TGTGCACAAT GTAAGGTTCC ATGGCATGCG GAGATGAATT GTCAGGAGTT TCAAGATTTG 
    AAACGTCCTA AAAAGGAACA AGATTTGGAT GATAAATTTT TGGAGTTGGC TAACAGAAAG 
    AAGTGGCAGA GATGCCCAAA TTGCTCTATC TATGTCAAGA GAAGTGATGG ATGTGAACAC 
    ATGAAATGCA GGTGTAAATG TAACTTCTGC TACCATTGTG GGGAGGAATG GAAGCATGGG 
    CATTTATGCG AGAAGAGAAA ATATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
    BMC genomics15312(2014 Apr)
    Tang H,Krishnakumar V,Bidwell S,Rosen B,Chan A,Zhou S,Gentzbittel L,Childs KL,Yandell M,Gundlach H,Mayer KF,Schwartz DC,Town CD


    The Medicago genome provides insight into the evolution of rhizobial symbioses.
    Nature480(7378)520-4(2011 Nov)
    Young ND,Debell? F,Oldroyd GE,Geurts R,Cannon SB,Udvardi MK,Benedito VA,Mayer KF,Gouzy J,Schoof H,Van de Peer Y,Proost S,Cook DR,Meyers BC,Spannagl M,Cheung F,De Mita S,Krishnakumar V,Gundlach H,Zhou S,Mudge J,Bharti AK,Murray JD,Naoumkina MA,Rosen B,Silverstein KA,Tang H,Rombauts S,Zhao PX,Zhou P,Barbe V,Bardou P,Bechner M,Bellec A,Berger A,Berg?s H,Bidwell S,Bisseling T,Choisne N,Couloux A,Denny R,Deshpande S,Dai X,Doyle JJ,Dudez AM,Farmer AD,Fouteau S,Franken C,Gibelin C,Gish J,Goldstein S,Gonz?lez AJ,Green PJ,Hallab A,Hartog M,Hua A,Humphray SJ,Jeong DH,Jing Y,J?cker A,Kenton SM,Kim DJ,Klee K,Lai H,Lang C,Lin S,Macmil SL,Magdelenat G,Matthews L,McCorrison J,Monaghan EL,Mun JH,Najar FZ,Nicholson C,Noirot C,O'Bleness M,Paule CR,Poulain J,Prion F,Qin B,Qu C,Retzel EF,Riddle C,Sallet E,Samain S,Samson N,Sanders I,Saurat O,Scarpelli C,Schiex T,Segurens B,Severin AJ,Sherrier DJ,Shi R,Sims S,Singer SR,Sinharoy S,Sterck L,Viollet A,Wang BB,Wang K,Wang M,Wang X,Warfsmann J,Weissenbach J,White DD,White JD,Wiley GB,Wincker P,Xing Y,Yang L,Yao Z,Ying F,Zhai J,Zhou L,Zuber A,D?nari? J,Dixon RA,May GD,Schwartz DC,Rogers J,Qu?tier F,Town CD,Roe BA


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