LOC25484591 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC25484591
Entrez Gene ID	25484591
Full Name	NB-ARC domain disease resistance protein
Gene Type	protein-coding
Organism	Medicago truncatula(barrel medic)

mRNA and Protein(s)

mRNA	Protein	Name
XM_013613444.1	XP_013468898.1	NB-ARC domain disease resistance protein
XM_013613444.2	XP_013468898.2	disease resistance protein RPM1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
Tang H, Krishnakumar V, Bidwell S, Rosen B, Chan A, Zhou S, Gentzbittel L, Childs KL, Yandell M, Gundlach H, Mayer KF, Schwartz DC, Town CD
BMC genomics15312(2014 Apr)

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Young ND, Debell? F, Oldroyd GE, Geurts R, Cannon SB, Udvardi MK, Benedito VA, Mayer KF, Gouzy J, Schoof H, Van de Peer Y, Proost S, Cook DR, Meyers BC, Spannagl M, Cheung F, De Mita S, Krishnakumar V, Gundlach H, Zhou S, Mudge J, Bharti AK, Murray JD, Naoumkina MA, Rosen B, Silverstein KA, Tang H, Rombauts S, Zhao PX, Zhou P, Barbe V, Bardou P, Bechner M, Bellec A, Berger A, Berg?s H, Bidwell S, Bisseling T, Choisne N, Couloux A, Denny R, Deshpande S, Dai X, Doyle JJ, Dudez AM, Farmer AD, Fouteau S, Franken C, Gibelin C, Gish J, Goldstein S, Gonz?lez AJ, Green PJ, Hallab A, Hartog M, Hua A, Humphray SJ, Jeong DH, Jing Y, J?cker A, Kenton SM, Kim DJ, Klee K, Lai H, Lang C, Lin S, Macmil SL, Magdelenat G, Matthews L, McCorrison J, Monaghan EL, Mun JH, Najar FZ, Nicholson C, Noirot C, O'Bleness M, Paule CR, Poulain J, Prion F, Qin B, Qu C, Retzel EF, Riddle C, Sallet E, Samain S, Samson N, Sanders I, Saurat O, Scarpelli C, Schiex T, Segurens B, Severin AJ, Sherrier DJ, Shi R, Sims S, Singer SR, Sinharoy S, Sterck L, Viollet A, Wang BB, Wang K, Wang M, Wang X, Warfsmann J, Weissenbach J, White DD, White JD, Wiley GB, Wincker P, Xing Y, Yang L, Yao Z, Ying F, Zhai J, Zhou L, Zuber A, D?nari? J, Dixon RA, May GD, Schwartz DC, Rogers J, Qu?tier F, Town CD, Roe BA
Nature480(7378)520-4(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC25484591 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC25484591 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe212232	XM_013613444.1 Latest version!	Medicago truncatula NB-ARC domain disease resistance protein partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OMe257947	XM_013613444.2 Latest version!	Medicago truncatula disease resistance protein RPM1 (LOC25484591), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC25484591 ( XM_013613444.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_013468898.1 Medicago truncatula NB-ARC domain disease resistance protein partial mRNA.

Price & Availability↑

CloneID	OMe212232
Clone ID Related Accession (Same CDS sequence)	XM_013613444.1
Accession Version	XM_013613444.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-24
Organism	Medicago truncatula(barrel medic)
Product	NB-ARC domain disease resistance protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_016407). ##Genome-Annotation-Data-START## Annotation Provider :: JCVI Annotation Version :: Mt4.0v2 Annotation Pipeline :: JCVI Eukaryotic Genome Annotation Pipeline Annotation Method :: EVidenceModeler (EVM), PASA Features Annotated :: Gene; mRNA; CDS; tRNA URL :: http://www.jcvi.org/medicago ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGCAGAAA TGGCAGTGTC TTTTGCTATT AAGCAACTGC TTCCATTGTT AACGGAAGAA 
ACCAAACTAC TGAAAGGTGT TCACAAAGAA TTTGCAGACA TTAAATATGA ACTGGAAAGC 
ATCCAAACCT TCCTTGAAGA TGCTGATAGA AGAGCTGCAG CTGAAGGAGA CAACACTACT 
GAAGGAGCCA AAATGTGGGT GAAGCAGGTT AGGGAAGTGG CTTTTCGTAT AGAAGATGTC 
ATTGATGATT ATATGATCCA TGTAGGGCAG CAACCTTGTG ATCCTGGATG CATATCTCCA 
CTCCATAAGG TTGCTCGTTT TCCCAAAACT ATGACTCCTC GCCGTCAAAT AGCTTCTGAG 
ATTCAAGACA TCAAGTCAGT TGTTTGTGGA ATCAAAGAAA GAAGTGAAAG ATATGGTTTC 
CAAATCCAAG GATCTAGCAG CTTTAGAGGA AACCAAAACG CCAAATGGAA CGATCAACGA 
ATGGCTGCTT TATACATCGA TGAAGCTGAA GTCGTAGGCT TTCAAGAACC CAAAAATAGA 
TTGATTGATT GGCTGGTAAA GGGAAGAGTA GAGCGCACTG TCATCTCTGT AGTTGGGATG 
GGAGGGCAAG GAAAAACCAC TCTTGCTAAA AAGGTTTTTG ATAGCAAAGA GGTTGTGGGA 
CACTTTGAAT GTCGTGTATG GATCACAGTG TCACAATCAA ACAACACTGA AGTATTGTCG 
AGACACATGT TGGAGAAGCT TTACGGACAA AAAGGAGAAA AACCTCCTAA AGGAATCACT 
GAAATGGATC GAGGTGCATT GATTTCTGAG TTGAGAAAGT ACTTGCAGAA AAAGAGGTAT 
GTTTTTGTGT TTGATGATGT ATGGAACACA AGTTTTTGGG ATGAGATTGA ATATGTTGTG 
AGTGATAATA AAAATGGTAG TAAGATATTT ATTACCACAA GAAATAAGGA TGTTGCCATG 
TATTGTAAGA AATCTTCATT TATTGAAGTG CATGAACTAC AACCATTAAC TGAAGAACAA 
TCAATTGATT TGTTCAATAA GAAGGCATTT CAATTTGACT TGGAAGGATG TTGTCCAAAA 
GAGCTCATTG ATATAGCTTT TGAAATTGCT AGGAAGTGCA AGGGCTTACC ACTAGCAATT 
GTGACTATTG GTGGTCTTTT ATCTACAAAG GGGAGAAATG CATTTGAATG GCAGAGATTT 
AGTGAAAATA TGACTGTAGA GCTAAGGAAT GATTCCCATT TGACCAGAAT AAAAAAGATT 
TTAGGTCTTA GCTATGATGA TTTGCCTTTT TATCTTAAAT CATGTTTGTT ATATTTTGGA 
ATGTATCCAG AAGATTATGA AGTTAAATCA AAGAGAATGA TACAACAATG GATAGCTGAA 
GGGTTTGTGA AAGAGGAAAG TGGGAAGACT TTGGAAGAAG TTGAAGAAAG ATATTTAACA 
GAGTTGATTC GTAGAAGTTT GGTGCAAGTA TCCTCAGTTA GCATTGATGG AAAAACTAGA 
AGTTGTTGTG TTCATGATCT GATACATATG ATGATCCTTG AAAAGTGTGA GGATTTAAGT 
TTTTGCAAGC ATTTCAATGA TGACGATCAT TCATCTTTAA GTGGAACTAT CAGACGCCTA 
TCAATAGCAA CAAACTCCGG TGATTTCAGA GCATGTATTG AAAACTCACA TATTCGGTCA 
CTATTTCTTT TCACAGATAA ATCAAATTAT TTAGAGGAAT CCATCCTGAA AAGAATATTC 
AAAAAGCAGA GGACACTGAA GGTGCTTCAT TTAGAAGATG TCGGATGTTT TGTTGATTAT 
AAACCTTTTA GATGTCTGAT CCACTTGAAA TATCTAAGCT TGAAGAATAG ATCCGGAAAA 
TACCTATGCA TTTCCAATAG ATCCCGAAAA TACACATATG CATTTCCCAA ATGGATTGGT 
ATTCTCCTAA ATCTTGAGAC CTTGGATCTA AGAGCTGCTT TTGGTTTTAT CATTATACCA 
AAGGAGATTA GCAAGCTTAG ACAGCTACGC CATCTTATAG GTTGCAGGAT GTCTTTGTTT 
CATTTGAAGG ATGTTATTGG ATGCATGGAA TCTCTACAAA CGTTGAGTGG AGTGAAAATT 
GGTAAGGGTG GAATAGAGTT GATTAAGGAG TTGGGAAAGC TAAGGCGGTT AAGGAAGTTG 
AGCTTGAATG ATGTGAGGGC AAGACATTCA AGCGCTTTAT CTTCCTCACT GAATGAGATG 
CGACACTTGG AGAAATTACG CATTGTGTCA AGTAGCGGAT ATGATATGGT TCATGATGTC 
ATTGATATGC ATTTGGTTTC ACCTCCACTA CCTATGCTTC GAAATCTTAA ACTATGTGTC 
AAGTTGGAGA AGTTTCCAGA GTGGATTCCT CAACTTAAAA ATCTTGTTAA GTTTGATTTA 
GCATATTCCT TATTAACTGA TGATCCAATT AAGTATCTAG AAAACATGCC AAACTTGTTG 
TCCCTCTCAA TCATCAACAA AGCTTATGAA GGTGAAAGTT TACATTTTCA TGATGGAGGT 
TGTCAAAATC TAAAGGAACT ATACATTGGA GACTGTCCCA ACATGAATTC AATTGTTATT 
GATAAAGGAG CATTACATTC TCTAAAAAAG TTTGAGTTAT TCGAGATCCC CAACCTCAAG 
ACTTCTGGAA TCCAACACTT AGAGAAGCTT GAAGTTCTCA ATGTTTGGGA TGTTCCCCTT 
GCAGAATTTA ATCTTAGTGA GGAAAATCAA TCCGAAACTT TAAGGACTAA GGAGATAGAA 
ATCACAAACC ATCCAAATCG GAGAACAGGT GAACATTTTC TTCCATATAA TTGTAAAACT 
CTATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013468898.1
CDS	1..2829
Translation	MAEMAVSFAIKQLLPLLTEETKLLKGVHKEFADIKYELESIQTFLEDADRRAAAEGDNTTEGAKMWVKQVREVAFRIEDVIDDYMIHVGQQPCDPGCISPLHKVARFPKTMTPRRQIASEIQDIKSVVCGIKERSERYGFQIQGSSSFRGNQNAKWNDQRMAALYIDEAEVVGFQEPKNRLIDWLVKGRVERTVISVVGMGGQGKTTLAKKVFDSKEVVGHFECRVWITVSQSNNTEVLSRHMLEKLYGQKGEKPPKGITEMDRGALISELRKYLQKKRYVFVFDDVWNTSFWDEIEYVVSDNKNGSKIFITTRNKDVAMYCKKSSFIEVHELQPLTEEQSIDLFNKKAFQFDLEGCCPKELIDIAFEIARKCKGLPLAIVTIGGLLSTKGRNAFEWQRFSENMTVELRNDSHLTRIKKILGLSYDDLPFYLKSCLLYFGMYPEDYEVKSKRMIQQWIAEGFVKEESGKTLEEVEERYLTELIRRSLVQVSSVSIDGKTRSCCVHDLIHMMILEKCEDLSFCKHFNDDDHSSLSGTIRRLSIATNSGDFRACIENSHIRSLFLFTDKSNYLEESILKRIFKKQRTLKVLHLEDVGCFVDYKPFRCLIHLKYLSLKNRSGKYLCISNRSRKYTYAFPKWIGILLNLETLDLRAAFGFIIIPKEISKLRQLRHLIGCRMSLFHLKDVIGCMESLQTLSGVKIGKGGIELIKELGKLRRLRKLSLNDVRARHSSALSSSLNEMRHLEKLRIVSSSGYDMVHDVIDMHLVSPPLPMLRNLKLCVKLEKFPEWIPQLKNLVKFDLAYSLLTDDPIKYLENMPNLLSLSIINKAYEGESLHFHDGGCQNLKELYIGDCPNMNSIVIDKGALHSLKKFELFEIPNLKTSGIQHLEKLEVLNVWDVPLAEFNLSEENQSETLRTKEIEITNHPNRRTGEHFLPYNCKTLL

Target ORF information:

RefSeq Version	XM_013613444.1
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula NB-ARC domain disease resistance protein partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013613444.1

ORF Insert Sequence:

ATGGCAGAAA TGGCAGTGTC TTTTGCTATT AAGCAACTGC TTCCATTGTT AACGGAAGAA 
ACCAAACTAC TGAAAGGTGT TCACAAAGAA TTTGCAGACA TTAAATATGA ACTGGAAAGC 
ATCCAAACCT TCCTTGAAGA TGCTGATAGA AGAGCTGCAG CTGAAGGAGA CAACACTACT 
GAAGGAGCCA AAATGTGGGT GAAGCAGGTT AGGGAAGTGG CTTTTCGTAT AGAAGATGTC 
ATTGATGATT ATATGATCCA TGTAGGGCAG CAACCTTGTG ATCCTGGATG CATATCTCCA 
CTCCATAAGG TTGCTCGTTT TCCCAAAACT ATGACTCCTC GCCGTCAAAT AGCTTCTGAG 
ATTCAAGACA TCAAGTCAGT TGTTTGTGGA ATCAAAGAAA GAAGTGAAAG ATATGGTTTC 
CAAATCCAAG GATCTAGCAG CTTTAGAGGA AACCAAAACG CCAAATGGAA CGATCAACGA 
ATGGCTGCTT TATACATCGA TGAAGCTGAA GTCGTAGGCT TTCAAGAACC CAAAAATAGA 
TTGATTGATT GGCTGGTAAA GGGAAGAGTA GAGCGCACTG TCATCTCTGT AGTTGGGATG 
GGAGGGCAAG GAAAAACCAC TCTTGCTAAA AAGGTTTTTG ATAGCAAAGA GGTTGTGGGA 
CACTTTGAAT GTCGTGTATG GATCACAGTG TCACAATCAA ACAACACTGA AGTATTGTCG 
AGACACATGT TGGAGAAGCT TTACGGACAA AAAGGAGAAA AACCTCCTAA AGGAATCACT 
GAAATGGATC GAGGTGCATT GATTTCTGAG TTGAGAAAGT ACTTGCAGAA AAAGAGGTAT 
GTTTTTGTGT TTGATGATGT ATGGAACACA AGTTTTTGGG ATGAGATTGA ATATGTTGTG 
AGTGATAATA AAAATGGTAG TAAGATATTT ATTACCACAA GAAATAAGGA TGTTGCCATG 
TATTGTAAGA AATCTTCATT TATTGAAGTG CATGAACTAC AACCATTAAC TGAAGAACAA 
TCAATTGATT TGTTCAATAA GAAGGCATTT CAATTTGACT TGGAAGGATG TTGTCCAAAA 
GAGCTCATTG ATATAGCTTT TGAAATTGCT AGGAAGTGCA AGGGCTTACC ACTAGCAATT 
GTGACTATTG GTGGTCTTTT ATCTACAAAG GGGAGAAATG CATTTGAATG GCAGAGATTT 
AGTGAAAATA TGACTGTAGA GCTAAGGAAT GATTCCCATT TGACCAGAAT AAAAAAGATT 
TTAGGTCTTA GCTATGATGA TTTGCCTTTT TATCTTAAAT CATGTTTGTT ATATTTTGGA 
ATGTATCCAG AAGATTATGA AGTTAAATCA AAGAGAATGA TACAACAATG GATAGCTGAA 
GGGTTTGTGA AAGAGGAAAG TGGGAAGACT TTGGAAGAAG TTGAAGAAAG ATATTTAACA 
GAGTTGATTC GTAGAAGTTT GGTGCAAGTA TCCTCAGTTA GCATTGATGG AAAAACTAGA 
AGTTGTTGTG TTCATGATCT GATACATATG ATGATCCTTG AAAAGTGTGA GGATTTAAGT 
TTTTGCAAGC ATTTCAATGA TGACGATCAT TCATCTTTAA GTGGAACTAT CAGACGCCTA 
TCAATAGCAA CAAACTCCGG TGATTTCAGA GCATGTATTG AAAACTCACA TATTCGGTCA 
CTATTTCTTT TCACAGATAA ATCAAATTAT TTAGAGGAAT CCATCCTGAA AAGAATATTC 
AAAAAGCAGA GGACACTGAA GGTGCTTCAT TTAGAAGATG TCGGATGTTT TGTTGATTAT 
AAACCTTTTA GATGTCTGAT CCACTTGAAA TATCTAAGCT TGAAGAATAG ATCCGGAAAA 
TACCTATGCA TTTCCAATAG ATCCCGAAAA TACACATATG CATTTCCCAA ATGGATTGGT 
ATTCTCCTAA ATCTTGAGAC CTTGGATCTA AGAGCTGCTT TTGGTTTTAT CATTATACCA 
AAGGAGATTA GCAAGCTTAG ACAGCTACGC CATCTTATAG GTTGCAGGAT GTCTTTGTTT 
CATTTGAAGG ATGTTATTGG ATGCATGGAA TCTCTACAAA CGTTGAGTGG AGTGAAAATT 
GGTAAGGGTG GAATAGAGTT GATTAAGGAG TTGGGAAAGC TAAGGCGGTT AAGGAAGTTG 
AGCTTGAATG ATGTGAGGGC AAGACATTCA AGCGCTTTAT CTTCCTCACT GAATGAGATG 
CGACACTTGG AGAAATTACG CATTGTGTCA AGTAGCGGAT ATGATATGGT TCATGATGTC 
ATTGATATGC ATTTGGTTTC ACCTCCACTA CCTATGCTTC GAAATCTTAA ACTATGTGTC 
AAGTTGGAGA AGTTTCCAGA GTGGATTCCT CAACTTAAAA ATCTTGTTAA GTTTGATTTA 
GCATATTCCT TATTAACTGA TGATCCAATT AAGTATCTAG AAAACATGCC AAACTTGTTG 
TCCCTCTCAA TCATCAACAA AGCTTATGAA GGTGAAAGTT TACATTTTCA TGATGGAGGT 
TGTCAAAATC TAAAGGAACT ATACATTGGA GACTGTCCCA ACATGAATTC AATTGTTATT 
GATAAAGGAG CATTACATTC TCTAAAAAAG TTTGAGTTAT TCGAGATCCC CAACCTCAAG 
ACTTCTGGAA TCCAACACTT AGAGAAGCTT GAAGTTCTCA ATGTTTGGGA TGTTCCCCTT 
GCAGAATTTA ATCTTAGTGA GGAAAATCAA TCCGAAACTT TAAGGACTAA GGAGATAGAA 
ATCACAAACC ATCCAAATCG GAGAACAGGT GAACATTTTC TTCCATATAA TTGTAAAACT 
CTATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC25484591 ( XM_013613444.2 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_013468898.2 Medicago truncatula disease resistance protein RPM1 (LOC25484591), mRNA.

Price & Availability↑

CloneID	OMe257947
Clone ID Related Accession (Same CDS sequence)	XM_013613444.2
Accession Version	XM_013613444.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2805bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	disease resistance protein RPM1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016407.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2018 this sequence version replaced XM_013613444.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAA TGGCAGTGTC TTTTGCTATT AAGCAACTGC TTCCATTGTT AACGGAAGAA 
ACCAAACTAC TGAAAGGTGT TCACAAAGAA TTTGCAGACA TTAAATATGA ACTGGAAAGC 
ATCCAAACCT TCCTTGAAGA TGCTGATAGA AGAGCTGCAG CTGAAGGAGA CAACACTACT 
GAAGGAGCCA AAATGTGGGT GAAGCAGGTT AGGGAAGTGG CTTTTCGTAT AGAAGATGTC 
ATTGATGATT ATATGATCCA TGTAGGGCAG CAACCTTGTG ATCCTGGATG CATATCTCCA 
CTCCATAAGG TTGCTCGTTT TCCCAAAACT ATGACTCCTC GCCGTCAAAT AGCTTCTGAG 
ATTCAAGACA TCAAGTCAGT TGTTTGTGGA ATCAAAGAAA GAAGTGAAAG ATATGGTTTC 
CAAATCCAAG GATCTAGCAG CTTTAGAGGA AACCAAAACG CCAAATGGAA CGATCAACGA 
ATGGCTGCTT TATACATCGA TGAAGCTGAA GTCGTAGGCT TTCAAGAACC CAAAAATAGA 
TTGATTGATT GGCTGGTAAA GGGAAGAGTA GAGCGCACTG TCATCTCTGT AGTTGGGATG 
GGAGGGCAAG GAAAAACCAC TCTTGCTAAA AAGGTTTTTG ATAGCAAAGA GGTTGTGGGA 
CACTTTGAAT GTCGTGTATG GATCACAGTG TCACAATCAA ACAACACTGA AGTATTGTCG 
AGACACATGT TGGAGAAGCT TTACGGACAA AAAGGAGAAA AACCTCCTAA AGGAATCACT 
GAAATGGATC GAGGTGCATT GATTTCTGAG TTGAGAAAGT ACTTGCAGAA AAAGAGGTAT 
GTTTTTGTGT TTGATGATGT ATGGAACACA AGTTTTTGGG ATGAGATTGA ATATGTTGTG 
AGTGATAATA AAAATGGTAG TAAGATATTT ATTACCACAA GAAATAAGGA TGTTGCCATG 
TATTGTAAGA AATCTTCATT TATTGAAGTG CATGAACTAC AACCATTAAC TGAAGAACAA 
TCAATTGATT TGTTCAATAA GAAGGCATTT CAATTTGACT TGGAAGGATG TTGTCCAAAA 
GAGCTCATTG ATATAGCTTT TGAAATTGCT AGGAAGTGCA AGGGCTTACC ACTAGCAATT 
GTGACTATTG GTGGTCTTTT ATCTACAAAG GGGAGAAATG CATTTGAATG GCAGAGATTT 
AGTGAAAATA TGACTGTAGA GCTAAGGAAT GATTCCCATT TGACCAGAAT AAAAAAGATT 
TTAGGTCTTA GCTATGATGA TTTGCCTTTT TATCTTAAAT CATGTTTGTT ATATTTTGGA 
ATGTATCCAG AAGATTATGA AGTTAAATCA AAGAGAATGA TACAACAATG GATAGCTGAA 
GGGTTTGTGA AAGAGGAAAG TGGGAAGACT TTGGAAGAAG TTGAAGAAAG ATATTTAACA 
GAGTTGATTC GTAGAAGTTT GGTGCAAGTA TCCTCAGTTA GCATTGATGG AAAAACTAGA 
AGTTGTTGTG TTCATGATCT GATACATATG ATGATCCTTG AAAAGTGTGA GGATTTAAGT 
TTTTGCAAGC ATTTCAATGA TGACGATCAT TCATCTTTAA GTGGAACTAT CAGACGCCTA 
TCAATAGCAA CAAACTCCGG TGATTTCAGA GCATGTATTG AAAACTCACA TATTCGGTCA 
CTATTTCTTT TCACAGATAA ATCAAATTAT TTAGAGGAAT CCATCCTGAA AAGAATATTC 
AAAAAGCAGA GGACACTGAA GGTGCTTCAT TTAGAAGATG TCGGATGTTT TGTTGATTAT 
AAACCTTTTA GATGTCTGAT CCACTTGAAA TATCTAAGCT TGAAGAATAG ATCCGGAAAA 
TACCTATGCA TTTCCAATAG ATCCCGAAAA TACACATATG CATTTCCCAA ATGGATTGGT 
ATTCTCCTAA ATCTTGAGAC CTTGGATCTA AGAGCTGCTT TTGGTTTTAT CATTATACCA 
AAGGAGATTA GCAAGCTTAG ACAGCTACGC CATCTTATAG GTTGCAGGAT GTCTTTGTTT 
CATTTGAAGG ATGTTATTGG ATGCATGGAA TCTCTACAAA CGTTGAGTGG AGTGAAAATT 
GGTAAGGGTG GAATAGAGTT GATTAAGGAG TTGGGAAAGC TAAGGCGGTT AAGGAAGTTG 
AGCTTGAATG ATGTGAGGGC AAGACATTCA AGCGCTTTAT CTTCCTCACT GAATGAGATG 
CGACACTTGG AGAAATTACG CATTGTGTCA AGTAGCGGAT ATGATATGGT TCATGATGTC 
ATTGATATGC ATTTGGTTTC ACCTCCACTA CCTATGCTTC GAAATCTTAA ACTATGTGTC 
AAGTTGGAGA AGTTTCCAGA GTGGATTCCT CAACTTAAAA ATCTTGTTAA GTTTGATTTA 
GCATATTCCT TATTAACTGA TGATCCAATT AAGTATCTAG AAAACATGCC AAACTTGTTG 
TCCCTCTCAA TCATCAACAA AGCTTATGAA GGTGAAAGTT TACATTTTCA TGATGGAGGT 
TGTCAAAATC TAAAGGAACT ATACATTGGA GACTGTCCCA ACATGAATTC AATTGTTATT 
GATAAAGGAG CATTACATTC TCTAAAAAAG TTTGAGTTAT TCGAGATCCC CAACCTCAAG 
ACTTCTGGAA TCCAACACTT AGAGAAGCTT GAAGTTCTCA ATGTTTGGGA TGTTCCCCTT 
GCAGAATTTA ATCTTAGTGA GGAAAATCAA TCCGAAACTT TAAGGACTAA GGAGATAGAA 
ATCACAAACC ATCCAAATCG GAGAACAGAA TTTTGGCGTC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013468898.2
CDS	210..3014
Translation	MAEMAVSFAIKQLLPLLTEETKLLKGVHKEFADIKYELESIQTFLEDADRRAAAEGDNTTEGAKMWVKQVREVAFRIEDVIDDYMIHVGQQPCDPGCISPLHKVARFPKTMTPRRQIASEIQDIKSVVCGIKERSERYGFQIQGSSSFRGNQNAKWNDQRMAALYIDEAEVVGFQEPKNRLIDWLVKGRVERTVISVVGMGGQGKTTLAKKVFDSKEVVGHFECRVWITVSQSNNTEVLSRHMLEKLYGQKGEKPPKGITEMDRGALISELRKYLQKKRYVFVFDDVWNTSFWDEIEYVVSDNKNGSKIFITTRNKDVAMYCKKSSFIEVHELQPLTEEQSIDLFNKKAFQFDLEGCCPKELIDIAFEIARKCKGLPLAIVTIGGLLSTKGRNAFEWQRFSENMTVELRNDSHLTRIKKILGLSYDDLPFYLKSCLLYFGMYPEDYEVKSKRMIQQWIAEGFVKEESGKTLEEVEERYLTELIRRSLVQVSSVSIDGKTRSCCVHDLIHMMILEKCEDLSFCKHFNDDDHSSLSGTIRRLSIATNSGDFRACIENSHIRSLFLFTDKSNYLEESILKRIFKKQRTLKVLHLEDVGCFVDYKPFRCLIHLKYLSLKNRSGKYLCISNRSRKYTYAFPKWIGILLNLETLDLRAAFGFIIIPKEISKLRQLRHLIGCRMSLFHLKDVIGCMESLQTLSGVKIGKGGIELIKELGKLRRLRKLSLNDVRARHSSALSSSLNEMRHLEKLRIVSSSGYDMVHDVIDMHLVSPPLPMLRNLKLCVKLEKFPEWIPQLKNLVKFDLAYSLLTDDPIKYLENMPNLLSLSIINKAYEGESLHFHDGGCQNLKELYIGDCPNMNSIVIDKGALHSLKKFELFEIPNLKTSGIQHLEKLEVLNVWDVPLAEFNLSEENQSETLRTKEIEITNHPNRRTEFWRR

Target ORF information:

RefSeq Version	XM_013613444.2
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula disease resistance protein RPM1 (LOC25484591), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013613444.2

ORF Insert Sequence:

ATGGCAGAAA TGGCAGTGTC TTTTGCTATT AAGCAACTGC TTCCATTGTT AACGGAAGAA 
ACCAAACTAC TGAAAGGTGT TCACAAAGAA TTTGCAGACA TTAAATATGA ACTGGAAAGC 
ATCCAAACCT TCCTTGAAGA TGCTGATAGA AGAGCTGCAG CTGAAGGAGA CAACACTACT 
GAAGGAGCCA AAATGTGGGT GAAGCAGGTT AGGGAAGTGG CTTTTCGTAT AGAAGATGTC 
ATTGATGATT ATATGATCCA TGTAGGGCAG CAACCTTGTG ATCCTGGATG CATATCTCCA 
CTCCATAAGG TTGCTCGTTT TCCCAAAACT ATGACTCCTC GCCGTCAAAT AGCTTCTGAG 
ATTCAAGACA TCAAGTCAGT TGTTTGTGGA ATCAAAGAAA GAAGTGAAAG ATATGGTTTC 
CAAATCCAAG GATCTAGCAG CTTTAGAGGA AACCAAAACG CCAAATGGAA CGATCAACGA 
ATGGCTGCTT TATACATCGA TGAAGCTGAA GTCGTAGGCT TTCAAGAACC CAAAAATAGA 
TTGATTGATT GGCTGGTAAA GGGAAGAGTA GAGCGCACTG TCATCTCTGT AGTTGGGATG 
GGAGGGCAAG GAAAAACCAC TCTTGCTAAA AAGGTTTTTG ATAGCAAAGA GGTTGTGGGA 
CACTTTGAAT GTCGTGTATG GATCACAGTG TCACAATCAA ACAACACTGA AGTATTGTCG 
AGACACATGT TGGAGAAGCT TTACGGACAA AAAGGAGAAA AACCTCCTAA AGGAATCACT 
GAAATGGATC GAGGTGCATT GATTTCTGAG TTGAGAAAGT ACTTGCAGAA AAAGAGGTAT 
GTTTTTGTGT TTGATGATGT ATGGAACACA AGTTTTTGGG ATGAGATTGA ATATGTTGTG 
AGTGATAATA AAAATGGTAG TAAGATATTT ATTACCACAA GAAATAAGGA TGTTGCCATG 
TATTGTAAGA AATCTTCATT TATTGAAGTG CATGAACTAC AACCATTAAC TGAAGAACAA 
TCAATTGATT TGTTCAATAA GAAGGCATTT CAATTTGACT TGGAAGGATG TTGTCCAAAA 
GAGCTCATTG ATATAGCTTT TGAAATTGCT AGGAAGTGCA AGGGCTTACC ACTAGCAATT 
GTGACTATTG GTGGTCTTTT ATCTACAAAG GGGAGAAATG CATTTGAATG GCAGAGATTT 
AGTGAAAATA TGACTGTAGA GCTAAGGAAT GATTCCCATT TGACCAGAAT AAAAAAGATT 
TTAGGTCTTA GCTATGATGA TTTGCCTTTT TATCTTAAAT CATGTTTGTT ATATTTTGGA 
ATGTATCCAG AAGATTATGA AGTTAAATCA AAGAGAATGA TACAACAATG GATAGCTGAA 
GGGTTTGTGA AAGAGGAAAG TGGGAAGACT TTGGAAGAAG TTGAAGAAAG ATATTTAACA 
GAGTTGATTC GTAGAAGTTT GGTGCAAGTA TCCTCAGTTA GCATTGATGG AAAAACTAGA 
AGTTGTTGTG TTCATGATCT GATACATATG ATGATCCTTG AAAAGTGTGA GGATTTAAGT 
TTTTGCAAGC ATTTCAATGA TGACGATCAT TCATCTTTAA GTGGAACTAT CAGACGCCTA 
TCAATAGCAA CAAACTCCGG TGATTTCAGA GCATGTATTG AAAACTCACA TATTCGGTCA 
CTATTTCTTT TCACAGATAA ATCAAATTAT TTAGAGGAAT CCATCCTGAA AAGAATATTC 
AAAAAGCAGA GGACACTGAA GGTGCTTCAT TTAGAAGATG TCGGATGTTT TGTTGATTAT 
AAACCTTTTA GATGTCTGAT CCACTTGAAA TATCTAAGCT TGAAGAATAG ATCCGGAAAA 
TACCTATGCA TTTCCAATAG ATCCCGAAAA TACACATATG CATTTCCCAA ATGGATTGGT 
ATTCTCCTAA ATCTTGAGAC CTTGGATCTA AGAGCTGCTT TTGGTTTTAT CATTATACCA 
AAGGAGATTA GCAAGCTTAG ACAGCTACGC CATCTTATAG GTTGCAGGAT GTCTTTGTTT 
CATTTGAAGG ATGTTATTGG ATGCATGGAA TCTCTACAAA CGTTGAGTGG AGTGAAAATT 
GGTAAGGGTG GAATAGAGTT GATTAAGGAG TTGGGAAAGC TAAGGCGGTT AAGGAAGTTG 
AGCTTGAATG ATGTGAGGGC AAGACATTCA AGCGCTTTAT CTTCCTCACT GAATGAGATG 
CGACACTTGG AGAAATTACG CATTGTGTCA AGTAGCGGAT ATGATATGGT TCATGATGTC 
ATTGATATGC ATTTGGTTTC ACCTCCACTA CCTATGCTTC GAAATCTTAA ACTATGTGTC 
AAGTTGGAGA AGTTTCCAGA GTGGATTCCT CAACTTAAAA ATCTTGTTAA GTTTGATTTA 
GCATATTCCT TATTAACTGA TGATCCAATT AAGTATCTAG AAAACATGCC AAACTTGTTG 
TCCCTCTCAA TCATCAACAA AGCTTATGAA GGTGAAAGTT TACATTTTCA TGATGGAGGT 
TGTCAAAATC TAAAGGAACT ATACATTGGA GACTGTCCCA ACATGAATTC AATTGTTATT 
GATAAAGGAG CATTACATTC TCTAAAAAAG TTTGAGTTAT TCGAGATCCC CAACCTCAAG 
ACTTCTGGAA TCCAACACTT AGAGAAGCTT GAAGTTCTCA ATGTTTGGGA TGTTCCCCTT 
GCAGAATTTA ATCTTAGTGA GGAAAATCAA TCCGAAACTT TAAGGACTAA GGAGATAGAA 
ATCACAAACC ATCCAAATCG GAGAACAGAA TTTTGGCGTC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
BMC genomics15312(2014 Apr)
Tang H,Krishnakumar V,Bidwell S,Rosen B,Chan A,Zhou S,Gentzbittel L,Childs KL,Yandell M,Gundlach H,Mayer KF,Schwartz DC,Town CD

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Nature480(7378)520-4(2011 Nov)
Young ND,Debell? F,Oldroyd GE,Geurts R,Cannon SB,Udvardi MK,Benedito VA,Mayer KF,Gouzy J,Schoof H,Van de Peer Y,Proost S,Cook DR,Meyers BC,Spannagl M,Cheung F,De Mita S,Krishnakumar V,Gundlach H,Zhou S,Mudge J,Bharti AK,Murray JD,Naoumkina MA,Rosen B,Silverstein KA,Tang H,Rombauts S,Zhao PX,Zhou P,Barbe V,Bardou P,Bechner M,Bellec A,Berger A,Berg?s H,Bidwell S,Bisseling T,Choisne N,Couloux A,Denny R,Deshpande S,Dai X,Doyle JJ,Dudez AM,Farmer AD,Fouteau S,Franken C,Gibelin C,Gish J,Goldstein S,Gonz?lez AJ,Green PJ,Hallab A,Hartog M,Hua A,Humphray SJ,Jeong DH,Jing Y,J?cker A,Kenton SM,Kim DJ,Klee K,Lai H,Lang C,Lin S,Macmil SL,Magdelenat G,Matthews L,McCorrison J,Monaghan EL,Mun JH,Najar FZ,Nicholson C,Noirot C,O'Bleness M,Paule CR,Poulain J,Prion F,Qin B,Qu C,Retzel EF,Riddle C,Sallet E,Samain S,Samson N,Sanders I,Saurat O,Scarpelli C,Schiex T,Segurens B,Severin AJ,Sherrier DJ,Shi R,Sims S,Singer SR,Sinharoy S,Sterck L,Viollet A,Wang BB,Wang K,Wang M,Wang X,Warfsmann J,Weissenbach J,White DD,White JD,Wiley GB,Wincker P,Xing Y,Yang L,Yao Z,Ying F,Zhai J,Zhou L,Zuber A,D?nari? J,Dixon RA,May GD,Schwartz DC,Rogers J,Qu?tier F,Town CD,Roe BA

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LOC25484591 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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