LOC11436615 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC11436615
Entrez Gene ID	11436615
Full Name	phospholipase D alpha 1
Gene Type	protein-coding
Organism	Medicago truncatula(barrel medic)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003603359.2	XP_003603407.1	phospholipase D alpha 1
XM_003603359.3	XP_003603407.1	phospholipase D alpha 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
Tang H, Krishnakumar V, Bidwell S, Rosen B, Chan A, Zhou S, Gentzbittel L, Childs KL, Yandell M, Gundlach H, Mayer KF, Schwartz DC, Town CD
BMC genomics15312(2014 Apr)

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Young ND, Debell? F, Oldroyd GE, Geurts R, Cannon SB, Udvardi MK, Benedito VA, Mayer KF, Gouzy J, Schoof H, Van de Peer Y, Proost S, Cook DR, Meyers BC, Spannagl M, Cheung F, De Mita S, Krishnakumar V, Gundlach H, Zhou S, Mudge J, Bharti AK, Murray JD, Naoumkina MA, Rosen B, Silverstein KA, Tang H, Rombauts S, Zhao PX, Zhou P, Barbe V, Bardou P, Bechner M, Bellec A, Berger A, Berg?s H, Bidwell S, Bisseling T, Choisne N, Couloux A, Denny R, Deshpande S, Dai X, Doyle JJ, Dudez AM, Farmer AD, Fouteau S, Franken C, Gibelin C, Gish J, Goldstein S, Gonz?lez AJ, Green PJ, Hallab A, Hartog M, Hua A, Humphray SJ, Jeong DH, Jing Y, J?cker A, Kenton SM, Kim DJ, Klee K, Lai H, Lang C, Lin S, Macmil SL, Magdelenat G, Matthews L, McCorrison J, Monaghan EL, Mun JH, Najar FZ, Nicholson C, Noirot C, O'Bleness M, Paule CR, Poulain J, Prion F, Qin B, Qu C, Retzel EF, Riddle C, Sallet E, Samain S, Samson N, Sanders I, Saurat O, Scarpelli C, Schiex T, Segurens B, Severin AJ, Sherrier DJ, Shi R, Sims S, Singer SR, Sinharoy S, Sterck L, Viollet A, Wang BB, Wang K, Wang M, Wang X, Warfsmann J, Weissenbach J, White DD, White JD, Wiley GB, Wincker P, Xing Y, Yang L, Yao Z, Ying F, Zhai J, Zhou L, Zuber A, D?nari? J, Dixon RA, May GD, Schwartz DC, Rogers J, Qu?tier F, Town CD, Roe BA
Nature480(7378)520-4(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC11436615 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC11436615 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe200514	XM_003603359.3 Latest version!	Medicago truncatula phospholipase D alpha 1 (LOC11436615), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OMe200514	XM_003603359.2	Medicago truncatula phospholipase D alpha 1 mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC11436615 ( XM_003603359.2 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_003603407.1 Medicago truncatula phospholipase D alpha 1 mRNA.

Price & Availability↑

CloneID	OMe200514
Clone ID Related Accession (Same CDS sequence)	XM_003603359.2 , XM_003603359.3
Accession Version	XM_003603359.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-24
Organism	Medicago truncatula(barrel medic)
Product	phospholipase D alpha 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_016409). On Aug 25, 2015 this sequence version replaced XM_003603359.1. ##Genome-Annotation-Data-START## Annotation Provider :: JCVI Annotation Version :: Mt4.0v2 Annotation Pipeline :: JCVI Eukaryotic Genome Annotation Pipeline Annotation Method :: EVidenceModeler (EVM), PASA Features Annotated :: Gene; mRNA; CDS; tRNA URL :: http://www.jcvi.org/medicago ##Genome-Annotation-Data-END##

ATGCCTCAGC TGCTACACGG AACACTTAAG GTAACCATAA GTGAAGTTGA TAGGTTACAA 
GCTGGATGTA ATTTGGATTT CATTCGAAAG GGAACAACAC ACAAGGGGAA GAGATTTCTA 
GCCCAAGTCA AGGGTTGTAT CTTGTGCCGG CCTGAGATTG TTGGGACAAG ACTCTATGCA 
ACAGTTGATT TAGATAAAGC AAGGGTTGGA CGAACCAGAA TGATAGGGAA TCAACCCTCA 
AACCCGAAAT GGAATGAGAC CTTTGAAATC TATTGTGCTC ATTACATATC AAACATTGTA 
TTCACTGTCA AAGATGATAA TCCGATTGGT GCAACTCTAA TAGGAAGAGC TTACATCCCT 
TCTGAAAAAA TTCTCAAAGG ACCGATAATT GACAGATGGG TTGAGATATT AGACGAAGAA 
GATCATCGTC CTGTACCAGG CGGTTCTAAA ATTCGTGTCA GCATGCAATT CTCTAGTGTC 
ATTGAGGATA GAAAATGTCT ATGGTCTCAA GGAATACACA TGCCATTTTT GGGAGTTCAG 
CGTACATTTT TCGACCAGAG ACAAGGTTGT AGTGTTACTT TATACCAAGA CGCTCACGTC 
CCTGAGAGTG TTCATCCGTG GATTCCAATA TCTGGAACAA AATATTATGT ACCTGGAAGA 
TGTTGGGAAG ATATTTACAA TGCCATTATG AATGCTAAAG TCTTTATTTA CATAACTGGT 
TGGTCTGTTC ATACAGAAAT AACCTTGATT AGGGACCCAA ACAAGACAAC AGAAAGTAGT 
ATCACCCTAG GAGAAATGCT CAAGAAAAAA GCCAACGAAG GTGTTAATGT TCTTGTGCTT 
GTTTGGGATG ACAGAACCTC TGTTCCGGCT TTTAAGAAAG ACGGTTTGAT GGGAACACAT 
GACCAAGAAA CTGCAGAGTA TTTTAAGAAC ACAAAGGTAC ATTGTGTTTT ATGTCCGCGT 
AACCCTGATG GTGGAAAAAG CATAGTTCAA GGTTTTGAAA CTTCAACTAT GTTCACTCAT 
CACCAAAAGA CTATAGTTGT TGATGGCCAT CATGTTGAAG CATCGCAGAA GCGAACAGTT 
ATCAGTTTCA TAGGCGGTAT TGATCTTTGC GATGGAAGAT ATGATACAAT GGAACATCCT 
TTGTTTTCAA CATTGAATAC AATCCATCAT GATGATTTTC ATCAACCAAA TTTCCCAGGT 
GCTTCTATTA AGAAAGGTGG TCCTAGAGAA CCGTGGCATG ATATTCACTG CAAGCTAGAA 
GGACCTATTG CATGGGATGT GTTGTACAAT TTTGAGCAGA GGTGGGAAAA GCAAGTTGGG 
AAGCAATTCC TATTCCCAAT TGAAGTGCTT GATAGAATTC TAATCCACCC TTCTGATGCA 
ATGCTATCAG AAGAAGATGG CGAAACTTGG AGTGTTCAGT TGTTTAGATC CATTGATGGT 
GGTGCTGTTT CTAACTTCCC TCAAGCACCA AATGAAGTTA TAGAATTAGG CCTTGTTAGT 
GGAAAAGACA ACATCATTGA TAGAAGCATT CAAGATGCCT ATATAAATGC TATACGAAGA 
GCAAAAAACT TCATCTACAT TGAAAATCAG TATTTCCTAG GGAGTTCCTA TGGTTGGCAT 
TCAACTGATA TTAAAGTTGA GGATATTGGT GCATTGCATC TCATACCAAA GGAGTTGTCA 
TTGAAGATCA TTAGCAAAAT TGAAGCCGGA GAGAGGTTTG TCGTGTATGT GGTGATTCCA 
ATGTGGCCAG AAGGTATACC TGAGAGTGCA TCAGTTCAGG CAATATTAGA TTGGCAAAGA 
AGGACCATGG AGATGATGTA TACTGAGATT GCTGATGCCA TAAAAAAGAA GAACATTGAA 
GCACACCCTC GTGATTATTT GACATTTTAT TGTCTTGGTA AAAGGGAGAG TAAGAAAGAT 
GGAGAGTACA CTCCTCCAGA GGAACCAGCG CCTGACAGTG ATTATCATAG AGCACAAAAG 
TCTAGAAGAT TCATGATCTA TGTTCATTCC AAGATGATGA TAGTGGATGA TGAATACATA 
GTGATTGGTT CAGCAAACAT AAACCAAAGA TCAATGGATG GTGGAAGAGA CACAGAGATT 
GCAATGGGTG CATTTCAACC TCATCATCTA GCATCCACAT CCAATGGCTC ACAAAGACCA 
CAAGGTCAGG TATATGGCTT CAGGAGAGCA TTATGGTATG AACACATTGG AGATAACAGC 
GATGATTTTG ACGAGCCTGA AAGGCTGGAA TGTGTAAAAC TTTTGAACCG CGTTGCTGAG 
AGGAATTGGA AATTATACTG TGATGATGCA TTAGATGAAA GTGTAAATAC TCACCTTTTG 
CGATATCCTG TTGAAGTGGG TGAAGATGGA AGTGTAACAT CACTTTCAGG GATGCAATAT 
TTTCCTGATA CAATGGCTTT GATTCTTGGT TCAAAATCTG ATTACCTTCC TCCCATACTT 
ACCACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003603407.1
CDS	111..2579
Translation	MPQLLHGTLKVTISEVDRLQAGCNLDFIRKGTTHKGKRFLAQVKGCILCRPEIVGTRLYATVDLDKARVGRTRMIGNQPSNPKWNETFEIYCAHYISNIVFTVKDDNPIGATLIGRAYIPSEKILKGPIIDRWVEILDEEDHRPVPGGSKIRVSMQFSSVIEDRKCLWSQGIHMPFLGVQRTFFDQRQGCSVTLYQDAHVPESVHPWIPISGTKYYVPGRCWEDIYNAIMNAKVFIYITGWSVHTEITLIRDPNKTTESSITLGEMLKKKANEGVNVLVLVWDDRTSVPAFKKDGLMGTHDQETAEYFKNTKVHCVLCPRNPDGGKSIVQGFETSTMFTHHQKTIVVDGHHVEASQKRTVISFIGGIDLCDGRYDTMEHPLFSTLNTIHHDDFHQPNFPGASIKKGGPREPWHDIHCKLEGPIAWDVLYNFEQRWEKQVGKQFLFPIEVLDRILIHPSDAMLSEEDGETWSVQLFRSIDGGAVSNFPQAPNEVIELGLVSGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSYGWHSTDIKVEDIGALHLIPKELSLKIISKIEAGERFVVYVVIPMWPEGIPESASVQAILDWQRRTMEMMYTEIADAIKKKNIEAHPRDYLTFYCLGKRESKKDGEYTPPEEPAPDSDYHRAQKSRRFMIYVHSKMMIVDDEYIVIGSANINQRSMDGGRDTEIAMGAFQPHHLASTSNGSQRPQGQVYGFRRALWYEHIGDNSDDFDEPERLECVKLLNRVAERNWKLYCDDALDESVNTHLLRYPVEVGEDGSVTSLSGMQYFPDTMALILGSKSDYLPPILTT

Target ORF information:

RefSeq Version	XM_003603359.2
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula phospholipase D alpha 1 mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003603359.2

ORF Insert Sequence:

ATGCCTCAGC TGCTACACGG AACACTTAAG GTAACCATAA GTGAAGTTGA TAGGTTACAA 
GCTGGATGTA ATTTGGATTT CATTCGAAAG GGAACAACAC ACAAGGGGAA GAGATTTCTA 
GCCCAAGTCA AGGGTTGTAT CTTGTGCCGG CCTGAGATTG TTGGGACAAG ACTCTATGCA 
ACAGTTGATT TAGATAAAGC AAGGGTTGGA CGAACCAGAA TGATAGGGAA TCAACCCTCA 
AACCCGAAAT GGAATGAGAC CTTTGAAATC TATTGTGCTC ATTACATATC AAACATTGTA 
TTCACTGTCA AAGATGATAA TCCGATTGGT GCAACTCTAA TAGGAAGAGC TTACATCCCT 
TCTGAAAAAA TTCTCAAAGG ACCGATAATT GACAGATGGG TTGAGATATT AGACGAAGAA 
GATCATCGTC CTGTACCAGG CGGTTCTAAA ATTCGTGTCA GCATGCAATT CTCTAGTGTC 
ATTGAGGATA GAAAATGTCT ATGGTCTCAA GGAATACACA TGCCATTTTT GGGAGTTCAG 
CGTACATTTT TCGACCAGAG ACAAGGTTGT AGTGTTACTT TATACCAAGA CGCTCACGTC 
CCTGAGAGTG TTCATCCGTG GATTCCAATA TCTGGAACAA AATATTATGT ACCTGGAAGA 
TGTTGGGAAG ATATTTACAA TGCCATTATG AATGCTAAAG TCTTTATTTA CATAACTGGT 
TGGTCTGTTC ATACAGAAAT AACCTTGATT AGGGACCCAA ACAAGACAAC AGAAAGTAGT 
ATCACCCTAG GAGAAATGCT CAAGAAAAAA GCCAACGAAG GTGTTAATGT TCTTGTGCTT 
GTTTGGGATG ACAGAACCTC TGTTCCGGCT TTTAAGAAAG ACGGTTTGAT GGGAACACAT 
GACCAAGAAA CTGCAGAGTA TTTTAAGAAC ACAAAGGTAC ATTGTGTTTT ATGTCCGCGT 
AACCCTGATG GTGGAAAAAG CATAGTTCAA GGTTTTGAAA CTTCAACTAT GTTCACTCAT 
CACCAAAAGA CTATAGTTGT TGATGGCCAT CATGTTGAAG CATCGCAGAA GCGAACAGTT 
ATCAGTTTCA TAGGCGGTAT TGATCTTTGC GATGGAAGAT ATGATACAAT GGAACATCCT 
TTGTTTTCAA CATTGAATAC AATCCATCAT GATGATTTTC ATCAACCAAA TTTCCCAGGT 
GCTTCTATTA AGAAAGGTGG TCCTAGAGAA CCGTGGCATG ATATTCACTG CAAGCTAGAA 
GGACCTATTG CATGGGATGT GTTGTACAAT TTTGAGCAGA GGTGGGAAAA GCAAGTTGGG 
AAGCAATTCC TATTCCCAAT TGAAGTGCTT GATAGAATTC TAATCCACCC TTCTGATGCA 
ATGCTATCAG AAGAAGATGG CGAAACTTGG AGTGTTCAGT TGTTTAGATC CATTGATGGT 
GGTGCTGTTT CTAACTTCCC TCAAGCACCA AATGAAGTTA TAGAATTAGG CCTTGTTAGT 
GGAAAAGACA ACATCATTGA TAGAAGCATT CAAGATGCCT ATATAAATGC TATACGAAGA 
GCAAAAAACT TCATCTACAT TGAAAATCAG TATTTCCTAG GGAGTTCCTA TGGTTGGCAT 
TCAACTGATA TTAAAGTTGA GGATATTGGT GCATTGCATC TCATACCAAA GGAGTTGTCA 
TTGAAGATCA TTAGCAAAAT TGAAGCCGGA GAGAGGTTTG TCGTGTATGT GGTGATTCCA 
ATGTGGCCAG AAGGTATACC TGAGAGTGCA TCAGTTCAGG CAATATTAGA TTGGCAAAGA 
AGGACCATGG AGATGATGTA TACTGAGATT GCTGATGCCA TAAAAAAGAA GAACATTGAA 
GCACACCCTC GTGATTATTT GACATTTTAT TGTCTTGGTA AAAGGGAGAG TAAGAAAGAT 
GGAGAGTACA CTCCTCCAGA GGAACCAGCG CCTGACAGTG ATTATCATAG AGCACAAAAG 
TCTAGAAGAT TCATGATCTA TGTTCATTCC AAGATGATGA TAGTGGATGA TGAATACATA 
GTGATTGGTT CAGCAAACAT AAACCAAAGA TCAATGGATG GTGGAAGAGA CACAGAGATT 
GCAATGGGTG CATTTCAACC TCATCATCTA GCATCCACAT CCAATGGCTC ACAAAGACCA 
CAAGGTCAGG TATATGGCTT CAGGAGAGCA TTATGGTATG AACACATTGG AGATAACAGC 
GATGATTTTG ACGAGCCTGA AAGGCTGGAA TGTGTAAAAC TTTTGAACCG CGTTGCTGAG 
AGGAATTGGA AATTATACTG TGATGATGCA TTAGATGAAA GTGTAAATAC TCACCTTTTG 
CGATATCCTG TTGAAGTGGG TGAAGATGGA AGTGTAACAT CACTTTCAGG GATGCAATAT 
TTTCCTGATA CAATGGCTTT GATTCTTGGT TCAAAATCTG ATTACCTTCC TCCCATACTT 
ACCACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC11436615 ( XM_003603359.3 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_003603407.1 Medicago truncatula phospholipase D alpha 1 (LOC11436615), mRNA.

Price & Availability↑

CloneID	OMe200514
Clone ID Related Accession (Same CDS sequence)	XM_003603359.2 , XM_003603359.3
Accession Version	XM_003603359.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	phospholipase D alpha 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016409.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2018 this sequence version replaced XM_003603359.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCAGC TGCTACACGG AACACTTAAG GTAACCATAA GTGAAGTTGA TAGGTTACAA 
GCTGGATGTA ATTTGGATTT CATTCGAAAG GGAACAACAC ACAAGGGGAA GAGATTTCTA 
GCCCAAGTCA AGGGTTGTAT CTTGTGCCGG CCTGAGATTG TTGGGACAAG ACTCTATGCA 
ACAGTTGATT TAGATAAAGC AAGGGTTGGA CGAACCAGAA TGATAGGGAA TCAACCCTCA 
AACCCGAAAT GGAATGAGAC CTTTGAAATC TATTGTGCTC ATTACATATC AAACATTGTA 
TTCACTGTCA AAGATGATAA TCCGATTGGT GCAACTCTAA TAGGAAGAGC TTACATCCCT 
TCTGAAAAAA TTCTCAAAGG ACCGATAATT GACAGATGGG TTGAGATATT AGACGAAGAA 
GATCATCGTC CTGTACCAGG CGGTTCTAAA ATTCGTGTCA GCATGCAATT CTCTAGTGTC 
ATTGAGGATA GAAAATGTCT ATGGTCTCAA GGAATACACA TGCCATTTTT GGGAGTTCAG 
CGTACATTTT TCGACCAGAG ACAAGGTTGT AGTGTTACTT TATACCAAGA CGCTCACGTC 
CCTGAGAGTG TTCATCCGTG GATTCCAATA TCTGGAACAA AATATTATGT ACCTGGAAGA 
TGTTGGGAAG ATATTTACAA TGCCATTATG AATGCTAAAG TCTTTATTTA CATAACTGGT 
TGGTCTGTTC ATACAGAAAT AACCTTGATT AGGGACCCAA ACAAGACAAC AGAAAGTAGT 
ATCACCCTAG GAGAAATGCT CAAGAAAAAA GCCAACGAAG GTGTTAATGT TCTTGTGCTT 
GTTTGGGATG ACAGAACCTC TGTTCCGGCT TTTAAGAAAG ACGGTTTGAT GGGAACACAT 
GACCAAGAAA CTGCAGAGTA TTTTAAGAAC ACAAAGGTAC ATTGTGTTTT ATGTCCGCGT 
AACCCTGATG GTGGAAAAAG CATAGTTCAA GGTTTTGAAA CTTCAACTAT GTTCACTCAT 
CACCAAAAGA CTATAGTTGT TGATGGCCAT CATGTTGAAG CATCGCAGAA GCGAACAGTT 
ATCAGTTTCA TAGGCGGTAT TGATCTTTGC GATGGAAGAT ATGATACAAT GGAACATCCT 
TTGTTTTCAA CATTGAATAC AATCCATCAT GATGATTTTC ATCAACCAAA TTTCCCAGGT 
GCTTCTATTA AGAAAGGTGG TCCTAGAGAA CCGTGGCATG ATATTCACTG CAAGCTAGAA 
GGACCTATTG CATGGGATGT GTTGTACAAT TTTGAGCAGA GGTGGGAAAA GCAAGTTGGG 
AAGCAATTCC TATTCCCAAT TGAAGTGCTT GATAGAATTC TAATCCACCC TTCTGATGCA 
ATGCTATCAG AAGAAGATGG CGAAACTTGG AGTGTTCAGT TGTTTAGATC CATTGATGGT 
GGTGCTGTTT CTAACTTCCC TCAAGCACCA AATGAAGTTA TAGAATTAGG CCTTGTTAGT 
GGAAAAGACA ACATCATTGA TAGAAGCATT CAAGATGCCT ATATAAATGC TATACGAAGA 
GCAAAAAACT TCATCTACAT TGAAAATCAG TATTTCCTAG GGAGTTCCTA TGGTTGGCAT 
TCAACTGATA TTAAAGTTGA GGATATTGGT GCATTGCATC TCATACCAAA GGAGTTGTCA 
TTGAAGATCA TTAGCAAAAT TGAAGCCGGA GAGAGGTTTG TCGTGTATGT GGTGATTCCA 
ATGTGGCCAG AAGGTATACC TGAGAGTGCA TCAGTTCAGG CAATATTAGA TTGGCAAAGA 
AGGACCATGG AGATGATGTA TACTGAGATT GCTGATGCCA TAAAAAAGAA GAACATTGAA 
GCACACCCTC GTGATTATTT GACATTTTAT TGTCTTGGTA AAAGGGAGAG TAAGAAAGAT 
GGAGAGTACA CTCCTCCAGA GGAACCAGCG CCTGACAGTG ATTATCATAG AGCACAAAAG 
TCTAGAAGAT TCATGATCTA TGTTCATTCC AAGATGATGA TAGTGGATGA TGAATACATA 
GTGATTGGTT CAGCAAACAT AAACCAAAGA TCAATGGATG GTGGAAGAGA CACAGAGATT 
GCAATGGGTG CATTTCAACC TCATCATCTA GCATCCACAT CCAATGGCTC ACAAAGACCA 
CAAGGTCAGG TATATGGCTT CAGGAGAGCA TTATGGTATG AACACATTGG AGATAACAGC 
GATGATTTTG ACGAGCCTGA AAGGCTGGAA TGTGTAAAAC TTTTGAACCG CGTTGCTGAG 
AGGAATTGGA AATTATACTG TGATGATGCA TTAGATGAAA GTGTAAATAC TCACCTTTTG 
CGATATCCTG TTGAAGTGGG TGAAGATGGA AGTGTAACAT CACTTTCAGG GATGCAATAT 
TTTCCTGATA CAATGGCTTT GATTCTTGGT TCAAAATCTG ATTACCTTCC TCCCATACTT 
ACCACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003603407.1
CDS	161..2629
Translation	MPQLLHGTLKVTISEVDRLQAGCNLDFIRKGTTHKGKRFLAQVKGCILCRPEIVGTRLYATVDLDKARVGRTRMIGNQPSNPKWNETFEIYCAHYISNIVFTVKDDNPIGATLIGRAYIPSEKILKGPIIDRWVEILDEEDHRPVPGGSKIRVSMQFSSVIEDRKCLWSQGIHMPFLGVQRTFFDQRQGCSVTLYQDAHVPESVHPWIPISGTKYYVPGRCWEDIYNAIMNAKVFIYITGWSVHTEITLIRDPNKTTESSITLGEMLKKKANEGVNVLVLVWDDRTSVPAFKKDGLMGTHDQETAEYFKNTKVHCVLCPRNPDGGKSIVQGFETSTMFTHHQKTIVVDGHHVEASQKRTVISFIGGIDLCDGRYDTMEHPLFSTLNTIHHDDFHQPNFPGASIKKGGPREPWHDIHCKLEGPIAWDVLYNFEQRWEKQVGKQFLFPIEVLDRILIHPSDAMLSEEDGETWSVQLFRSIDGGAVSNFPQAPNEVIELGLVSGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSYGWHSTDIKVEDIGALHLIPKELSLKIISKIEAGERFVVYVVIPMWPEGIPESASVQAILDWQRRTMEMMYTEIADAIKKKNIEAHPRDYLTFYCLGKRESKKDGEYTPPEEPAPDSDYHRAQKSRRFMIYVHSKMMIVDDEYIVIGSANINQRSMDGGRDTEIAMGAFQPHHLASTSNGSQRPQGQVYGFRRALWYEHIGDNSDDFDEPERLECVKLLNRVAERNWKLYCDDALDESVNTHLLRYPVEVGEDGSVTSLSGMQYFPDTMALILGSKSDYLPPILTT

Target ORF information:

RefSeq Version	XM_003603359.3
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula phospholipase D alpha 1 (LOC11436615), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003603359.3

ORF Insert Sequence:

ATGCCTCAGC TGCTACACGG AACACTTAAG GTAACCATAA GTGAAGTTGA TAGGTTACAA 
GCTGGATGTA ATTTGGATTT CATTCGAAAG GGAACAACAC ACAAGGGGAA GAGATTTCTA 
GCCCAAGTCA AGGGTTGTAT CTTGTGCCGG CCTGAGATTG TTGGGACAAG ACTCTATGCA 
ACAGTTGATT TAGATAAAGC AAGGGTTGGA CGAACCAGAA TGATAGGGAA TCAACCCTCA 
AACCCGAAAT GGAATGAGAC CTTTGAAATC TATTGTGCTC ATTACATATC AAACATTGTA 
TTCACTGTCA AAGATGATAA TCCGATTGGT GCAACTCTAA TAGGAAGAGC TTACATCCCT 
TCTGAAAAAA TTCTCAAAGG ACCGATAATT GACAGATGGG TTGAGATATT AGACGAAGAA 
GATCATCGTC CTGTACCAGG CGGTTCTAAA ATTCGTGTCA GCATGCAATT CTCTAGTGTC 
ATTGAGGATA GAAAATGTCT ATGGTCTCAA GGAATACACA TGCCATTTTT GGGAGTTCAG 
CGTACATTTT TCGACCAGAG ACAAGGTTGT AGTGTTACTT TATACCAAGA CGCTCACGTC 
CCTGAGAGTG TTCATCCGTG GATTCCAATA TCTGGAACAA AATATTATGT ACCTGGAAGA 
TGTTGGGAAG ATATTTACAA TGCCATTATG AATGCTAAAG TCTTTATTTA CATAACTGGT 
TGGTCTGTTC ATACAGAAAT AACCTTGATT AGGGACCCAA ACAAGACAAC AGAAAGTAGT 
ATCACCCTAG GAGAAATGCT CAAGAAAAAA GCCAACGAAG GTGTTAATGT TCTTGTGCTT 
GTTTGGGATG ACAGAACCTC TGTTCCGGCT TTTAAGAAAG ACGGTTTGAT GGGAACACAT 
GACCAAGAAA CTGCAGAGTA TTTTAAGAAC ACAAAGGTAC ATTGTGTTTT ATGTCCGCGT 
AACCCTGATG GTGGAAAAAG CATAGTTCAA GGTTTTGAAA CTTCAACTAT GTTCACTCAT 
CACCAAAAGA CTATAGTTGT TGATGGCCAT CATGTTGAAG CATCGCAGAA GCGAACAGTT 
ATCAGTTTCA TAGGCGGTAT TGATCTTTGC GATGGAAGAT ATGATACAAT GGAACATCCT 
TTGTTTTCAA CATTGAATAC AATCCATCAT GATGATTTTC ATCAACCAAA TTTCCCAGGT 
GCTTCTATTA AGAAAGGTGG TCCTAGAGAA CCGTGGCATG ATATTCACTG CAAGCTAGAA 
GGACCTATTG CATGGGATGT GTTGTACAAT TTTGAGCAGA GGTGGGAAAA GCAAGTTGGG 
AAGCAATTCC TATTCCCAAT TGAAGTGCTT GATAGAATTC TAATCCACCC TTCTGATGCA 
ATGCTATCAG AAGAAGATGG CGAAACTTGG AGTGTTCAGT TGTTTAGATC CATTGATGGT 
GGTGCTGTTT CTAACTTCCC TCAAGCACCA AATGAAGTTA TAGAATTAGG CCTTGTTAGT 
GGAAAAGACA ACATCATTGA TAGAAGCATT CAAGATGCCT ATATAAATGC TATACGAAGA 
GCAAAAAACT TCATCTACAT TGAAAATCAG TATTTCCTAG GGAGTTCCTA TGGTTGGCAT 
TCAACTGATA TTAAAGTTGA GGATATTGGT GCATTGCATC TCATACCAAA GGAGTTGTCA 
TTGAAGATCA TTAGCAAAAT TGAAGCCGGA GAGAGGTTTG TCGTGTATGT GGTGATTCCA 
ATGTGGCCAG AAGGTATACC TGAGAGTGCA TCAGTTCAGG CAATATTAGA TTGGCAAAGA 
AGGACCATGG AGATGATGTA TACTGAGATT GCTGATGCCA TAAAAAAGAA GAACATTGAA 
GCACACCCTC GTGATTATTT GACATTTTAT TGTCTTGGTA AAAGGGAGAG TAAGAAAGAT 
GGAGAGTACA CTCCTCCAGA GGAACCAGCG CCTGACAGTG ATTATCATAG AGCACAAAAG 
TCTAGAAGAT TCATGATCTA TGTTCATTCC AAGATGATGA TAGTGGATGA TGAATACATA 
GTGATTGGTT CAGCAAACAT AAACCAAAGA TCAATGGATG GTGGAAGAGA CACAGAGATT 
GCAATGGGTG CATTTCAACC TCATCATCTA GCATCCACAT CCAATGGCTC ACAAAGACCA 
CAAGGTCAGG TATATGGCTT CAGGAGAGCA TTATGGTATG AACACATTGG AGATAACAGC 
GATGATTTTG ACGAGCCTGA AAGGCTGGAA TGTGTAAAAC TTTTGAACCG CGTTGCTGAG 
AGGAATTGGA AATTATACTG TGATGATGCA TTAGATGAAA GTGTAAATAC TCACCTTTTG 
CGATATCCTG TTGAAGTGGG TGAAGATGGA AGTGTAACAT CACTTTCAGG GATGCAATAT 
TTTCCTGATA CAATGGCTTT GATTCTTGGT TCAAAATCTG ATTACCTTCC TCCCATACTT 
ACCACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
BMC genomics15312(2014 Apr)
Tang H,Krishnakumar V,Bidwell S,Rosen B,Chan A,Zhou S,Gentzbittel L,Childs KL,Yandell M,Gundlach H,Mayer KF,Schwartz DC,Town CD

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Nature480(7378)520-4(2011 Nov)
Young ND,Debell? F,Oldroyd GE,Geurts R,Cannon SB,Udvardi MK,Benedito VA,Mayer KF,Gouzy J,Schoof H,Van de Peer Y,Proost S,Cook DR,Meyers BC,Spannagl M,Cheung F,De Mita S,Krishnakumar V,Gundlach H,Zhou S,Mudge J,Bharti AK,Murray JD,Naoumkina MA,Rosen B,Silverstein KA,Tang H,Rombauts S,Zhao PX,Zhou P,Barbe V,Bardou P,Bechner M,Bellec A,Berger A,Berg?s H,Bidwell S,Bisseling T,Choisne N,Couloux A,Denny R,Deshpande S,Dai X,Doyle JJ,Dudez AM,Farmer AD,Fouteau S,Franken C,Gibelin C,Gish J,Goldstein S,Gonz?lez AJ,Green PJ,Hallab A,Hartog M,Hua A,Humphray SJ,Jeong DH,Jing Y,J?cker A,Kenton SM,Kim DJ,Klee K,Lai H,Lang C,Lin S,Macmil SL,Magdelenat G,Matthews L,McCorrison J,Monaghan EL,Mun JH,Najar FZ,Nicholson C,Noirot C,O'Bleness M,Paule CR,Poulain J,Prion F,Qin B,Qu C,Retzel EF,Riddle C,Sallet E,Samain S,Samson N,Sanders I,Saurat O,Scarpelli C,Schiex T,Segurens B,Severin AJ,Sherrier DJ,Shi R,Sims S,Singer SR,Sinharoy S,Sterck L,Viollet A,Wang BB,Wang K,Wang M,Wang X,Warfsmann J,Weissenbach J,White DD,White JD,Wiley GB,Wincker P,Xing Y,Yang L,Yao Z,Ying F,Zhai J,Zhou L,Zuber A,D?nari? J,Dixon RA,May GD,Schwartz DC,Rogers J,Qu?tier F,Town CD,Roe BA

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LOC11436615 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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