LOC11413331 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC11413331
Entrez Gene ID	11413331
Full Name	NBS-LRR type disease resistance protein
Gene Type	protein-coding
Organism	Medicago truncatula(barrel medic)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003598360.2	XP_003598408.2	NBS-LRR type disease resistance protein
XM_024779532.1	XP_024635300.1	disease resistance protein RPM1
XM_024779534.1	XP_024635302.1	disease resistance protein RPM1
XM_003598360.3	XP_003598408.2	disease resistance protein RPM1
XM_024779533.1	XP_024635301.1	disease resistance protein RPM1
XM_024779535.1	XP_024635303.1	disease resistance protein RPM1
XM_024779536.1	XP_024635304.1	disease resistance protein RPM1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
Tang H, Krishnakumar V, Bidwell S, Rosen B, Chan A, Zhou S, Gentzbittel L, Childs KL, Yandell M, Gundlach H, Mayer KF, Schwartz DC, Town CD
BMC genomics15312(2014 Apr)

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Young ND, Debell? F, Oldroyd GE, Geurts R, Cannon SB, Udvardi MK, Benedito VA, Mayer KF, Gouzy J, Schoof H, Van de Peer Y, Proost S, Cook DR, Meyers BC, Spannagl M, Cheung F, De Mita S, Krishnakumar V, Gundlach H, Zhou S, Mudge J, Bharti AK, Murray JD, Naoumkina MA, Rosen B, Silverstein KA, Tang H, Rombauts S, Zhao PX, Zhou P, Barbe V, Bardou P, Bechner M, Bellec A, Berger A, Berg?s H, Bidwell S, Bisseling T, Choisne N, Couloux A, Denny R, Deshpande S, Dai X, Doyle JJ, Dudez AM, Farmer AD, Fouteau S, Franken C, Gibelin C, Gish J, Goldstein S, Gonz?lez AJ, Green PJ, Hallab A, Hartog M, Hua A, Humphray SJ, Jeong DH, Jing Y, J?cker A, Kenton SM, Kim DJ, Klee K, Lai H, Lang C, Lin S, Macmil SL, Magdelenat G, Matthews L, McCorrison J, Monaghan EL, Mun JH, Najar FZ, Nicholson C, Noirot C, O'Bleness M, Paule CR, Poulain J, Prion F, Qin B, Qu C, Retzel EF, Riddle C, Sallet E, Samain S, Samson N, Sanders I, Saurat O, Scarpelli C, Schiex T, Segurens B, Severin AJ, Sherrier DJ, Shi R, Sims S, Singer SR, Sinharoy S, Sterck L, Viollet A, Wang BB, Wang K, Wang M, Wang X, Warfsmann J, Weissenbach J, White DD, White JD, Wiley GB, Wincker P, Xing Y, Yang L, Yao Z, Ying F, Zhai J, Zhou L, Zuber A, D?nari? J, Dixon RA, May GD, Schwartz DC, Rogers J, Qu?tier F, Town CD, Roe BA
Nature480(7378)520-4(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC11413331 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC11413331 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe193250	XM_024779534.1 Latest version!	Medicago truncatula disease resistance protein RPM1 (LOC11413331), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OMe193250	XM_003598360.3 Latest version!	Medicago truncatula disease resistance protein RPM1 (LOC11413331), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OMe193250	XM_024779536.1 Latest version!	Medicago truncatula disease resistance protein RPM1 (LOC11413331), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OMe193250	XM_024779532.1 Latest version!	Medicago truncatula disease resistance protein RPM1 (LOC11413331), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OMe193250	XM_024779535.1 Latest version!	Medicago truncatula disease resistance protein RPM1 (LOC11413331), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OMe193250	XM_024779533.1 Latest version!	Medicago truncatula disease resistance protein RPM1 (LOC11413331), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OMe193250	XM_003598360.2	Medicago truncatula NBS-LRR type disease resistance protein mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC11413331 ( XM_003598360.2 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_003598408.2 Medicago truncatula NBS-LRR type disease resistance protein mRNA.

Price & Availability↑

CloneID	OMe193250
Clone ID Related Accession (Same CDS sequence)	XM_003598360.2 , XM_024779532.1 , XM_024779534.1 , XM_003598360.3 , XM_024779533.1 , XM_024779535.1 , XM_024779536.1
Accession Version	XM_003598360.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-24
Organism	Medicago truncatula(barrel medic)
Product	NBS-LRR type disease resistance protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_016409). On Aug 25, 2015 this sequence version replaced XM_003598360.1. ##Genome-Annotation-Data-START## Annotation Provider :: JCVI Annotation Version :: Mt4.0v2 Annotation Pipeline :: JCVI Eukaryotic Genome Annotation Pipeline Annotation Method :: EVidenceModeler (EVM), PASA Features Annotated :: Gene; mRNA; CDS; tRNA URL :: http://www.jcvi.org/medicago ##Genome-Annotation-Data-END##

ATGGCAGAAA CTGCAGTATC GTTTGTCCTG GGGGAAGTGT TCCAGTTCCT AAAAGAAGAA 
ACAAATCTTC TGAGAAGTGT ACACAGAGAT TTTTCAGACA TCAAAGATGA ACTTGAGAGC 
ATTCAAGTCT TCCTCAAAGA TGCAGACAGA AGGGCTGCGG ATGAAGCAGA CACTAACGAT 
GGAATAAGAA CTTGGGTGAA GCAGCTGAGA GAAGCATCAT TTCGCATTGA AGATGTCATT 
GATGAATACC TTCGGCTAAT GCATAGGGCC AATCCTCCTA GATGCGGATC TTTAGTCGGC 
AAGATTGCAA GGCAAATCAA AACTTTGATT CCCCGTCATC ATATAGCATC TGAAATTCAG 
GACATTAAAT TATCAATTCG TGGAATCAAG GAAAGAAGTG AGAGGTATAA CTTTCAAATT 
TCACACGAAC AAGGAACTTC AAGCAGCATT AGCATAGGAG AGAGAGAAAA TGGAAAATGG 
CATGATCCTC GACTGTCTTC TCTTTTTATT GAAGAAACAG AAGTTGTTGG TATTGAAGTC 
CCAAAAGAAG AACTGTCTGG TTGGTTATTG GATGGTGAAG CTGAGCGCAC GGTGATATCC 
GTGGTAGGAA TGGGAGGCCT AGGAAAAACC ACTCTTGCCA AGCTAGTTTT TGAAAGCAAA 
AAGGTATCAG CACACTTTGA TTGTCGTGCT TGCGTCACAG TTTCTCAATC TTACACTGTG 
AGAGGTTTGT TGATAAACAT GATGGAGCAA TTCTGTCGGA GTACTAAAGA CTCTCTTCTG 
CAGATGTTAC ACAAAATGGA TGACCAGTCA CTAATTATAG AAGTGAGGCA ATACCTGCAA 
CATAAGAGGT ATTTGATATT CTTTGATGAC GTATGGCAAG AAGATTTTGC GGACCAGGTT 
CAATTTGCCA TGCCAAATAA CAATAAAGGT AGTAGGATTA TCATCACCAC TAGAATGATG 
TATGTTGCAG ATTTTTTCAA AAAATCCTTT CTAACTCATA TTCACAACCT GCAACTTTTG 
CCTCCAAACA AAGCATGGGA ACTTTTCTGC AAGAGAGTAT TTAGATATGA GCTTGGTGGA 
CATTGTCCAT CAGAACTTGA GGCTGTGTCA AAAGAAATTG TTCAAAAGTG CAGGCAGCTA 
CCTCTAGCAA TTGTAGCTAT TGGTGGTTTA CTTTCAACAA AATCTAAAAC AATGATTGAA 
TGGCAAAAGG TGAGTGAAAA TCTGAGTCTG GAATTAGGAC GTAATGCCCA TTTAACGTGT 
CTAGTAAAGA TTCTATCACT TAGTTATGAT GGACTTCCTT ATTACTTGAA ACCATGCATT 
TTGTATTTTG GTTTATACCC AGAGGACTAT GCAATTAATC ATAGGAGATT AACTCGACAA 
TGGATAGCTG AAGGGTTTGT AAAATTGGAA GAGAGAAGAA CTCCAGAGCA AGTTGCAGAA 
GAGTACTTGT CTGAATTGAT TCAGAGAAGT TTGGTTCAGG TCTCAAATGT TGGCTTTGAA 
GGAAAAGTTC AAACATGTCA AGTCCATGAT TTAATGCGGG AATTGATCAT TAGAAAAATG 
AAGGATTTAA ATTTCGGTCA TTGCATGCAT GAAGATAGTG AATCAGTTGC ACTTGGAAGA 
ACTAGACGCC TATCTATAGC CACTAATCCC AACAATGTGT TCAGGAGCAC TAATAATTTA 
CAATTTCGGG CTATTTATGT GTTTGAAGAA GATGGATCAT TGGAGCATTT CATGGGTAAA 
GTTTGTTCGC AGTCAAGAAT TTTGAAGGTC CTTGACATTC AAGGGACGTC GTTGAATCAT 
ATCCCTAAAA ATTTGGGGAA TCTTTTTCAC TTGCGGTACA TTAACCTAAG GAATACAAAT 
GTAAAGGCTC TTCCTAAATC TATTGGTGAG TTACATAACT TAGAGACATT GGATTTAAGA 
GAAACACTAG TGCATGAGAT ACCTAGTGAA ATAAACAAGC TGACAAAGTT ACGGCATCTG 
CTTGCTTTTC ATCGCAACTA TGAACAAAAA TATTCTGCTT TGGGCTCTAC AACTGGCGTG 
CTGATTGAAA AAGGTATTAA AAACATGATT TCCCTAAAAA ACCTTTATTA TGTGGAGGTA 
GATCATGGGG GAGTAGATCT CATTGAAGAG ATGAAAATGC TAAGGCAGTT AAGGAAGTTA 
GGTTTAAAGC ATGTAAGAAG GGAACATGGG AATGCCATAA GTGCTGCAGT ACAAGAGATG 
CAACACCTTG AATCACTTAA TATCACTGCA ATAGAAGAGG ATGAAATTAT TGACTTAAAC 
TTTGCGTCAA CCCCACCAAA ACTTCAACGG CTCCATTTGA AAGCTAGACT AGAGAAGTTT 
CCTGATTGGA TTCCAAAATT TGAGTGTCTT GTTCAGATTT GTCTGGCTTT ATCCAAGCTG 
AAAGATGATC CGTTGCAGTC ACTGAAAAAT TTGCCAAATC TTCTGAAGCT CAATTTGTTG 
GAGAATGCCT TTGATGGTGA AATTTTGCAT TTTCAAAATG GAGGATTTCA AATATTGAAA 
GAACTGATTC TCAGTCACTT GAATAGACCA AATTCTATCC TTATAGAGAA AGGAGCTTTG 
CTCTCTCTTG AAAATCTTAA ATTGGAAAGG ATCCCCAAAC TAAAGGATGT GCCTTCTGGC 
ATCAAACACT TGGATAAGCT CAAAGTCATT GACTTAGTTG ATATGCCAGA TGAATTTGTT 
AAGAGCATTG ATCCAGACGG AGGACAAGAC CATTGGATTA TAAACCATGT TCCTATTGTG 
TTCATTCGTC AATGGTTTGG CCCAAAATAC TATGATTATG AGGTTCGCAC TATTAACTCA 
TCCACCAAAG AAGTCCTGAG AGATGAGAAT GCTAAGGTGT CTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003598408.2
CDS	660..3527
Translation	MAETAVSFVLGEVFQFLKEETNLLRSVHRDFSDIKDELESIQVFLKDADRRAADEADTNDGIRTWVKQLREASFRIEDVIDEYLRLMHRANPPRCGSLVGKIARQIKTLIPRHHIASEIQDIKLSIRGIKERSERYNFQISHEQGTSSSISIGERENGKWHDPRLSSLFIEETEVVGIEVPKEELSGWLLDGEAERTVISVVGMGGLGKTTLAKLVFESKKVSAHFDCRACVTVSQSYTVRGLLINMMEQFCRSTKDSLLQMLHKMDDQSLIIEVRQYLQHKRYLIFFDDVWQEDFADQVQFAMPNNNKGSRIIITTRMMYVADFFKKSFLTHIHNLQLLPPNKAWELFCKRVFRYELGGHCPSELEAVSKEIVQKCRQLPLAIVAIGGLLSTKSKTMIEWQKVSENLSLELGRNAHLTCLVKILSLSYDGLPYYLKPCILYFGLYPEDYAINHRRLTRQWIAEGFVKLEERRTPEQVAEEYLSELIQRSLVQVSNVGFEGKVQTCQVHDLMRELIIRKMKDLNFGHCMHEDSESVALGRTRRLSIATNPNNVFRSTNNLQFRAIYVFEEDGSLEHFMGKVCSQSRILKVLDIQGTSLNHIPKNLGNLFHLRYINLRNTNVKALPKSIGELHNLETLDLRETLVHEIPSEINKLTKLRHLLAFHRNYEQKYSALGSTTGVLIEKGIKNMISLKNLYYVEVDHGGVDLIEEMKMLRQLRKLGLKHVRREHGNAISAAVQEMQHLESLNITAIEEDEIIDLNFASTPPKLQRLHLKARLEKFPDWIPKFECLVQICLALSKLKDDPLQSLKNLPNLLKLNLLENAFDGEILHFQNGGFQILKELILSHLNRPNSILIEKGALLSLENLKLERIPKLKDVPSGIKHLDKLKVIDLVDMPDEFVKSIDPDGGQDHWIINHVPIVFIRQWFGPKYYDYEVRTINSSTKEVLRDENAKVSS

Target ORF information:

RefSeq Version	XM_003598360.2
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula NBS-LRR type disease resistance protein mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003598360.2

ORF Insert Sequence:

ATGGCAGAAA CTGCAGTATC GTTTGTCCTG GGGGAAGTGT TCCAGTTCCT AAAAGAAGAA 
ACAAATCTTC TGAGAAGTGT ACACAGAGAT TTTTCAGACA TCAAAGATGA ACTTGAGAGC 
ATTCAAGTCT TCCTCAAAGA TGCAGACAGA AGGGCTGCGG ATGAAGCAGA CACTAACGAT 
GGAATAAGAA CTTGGGTGAA GCAGCTGAGA GAAGCATCAT TTCGCATTGA AGATGTCATT 
GATGAATACC TTCGGCTAAT GCATAGGGCC AATCCTCCTA GATGCGGATC TTTAGTCGGC 
AAGATTGCAA GGCAAATCAA AACTTTGATT CCCCGTCATC ATATAGCATC TGAAATTCAG 
GACATTAAAT TATCAATTCG TGGAATCAAG GAAAGAAGTG AGAGGTATAA CTTTCAAATT 
TCACACGAAC AAGGAACTTC AAGCAGCATT AGCATAGGAG AGAGAGAAAA TGGAAAATGG 
CATGATCCTC GACTGTCTTC TCTTTTTATT GAAGAAACAG AAGTTGTTGG TATTGAAGTC 
CCAAAAGAAG AACTGTCTGG TTGGTTATTG GATGGTGAAG CTGAGCGCAC GGTGATATCC 
GTGGTAGGAA TGGGAGGCCT AGGAAAAACC ACTCTTGCCA AGCTAGTTTT TGAAAGCAAA 
AAGGTATCAG CACACTTTGA TTGTCGTGCT TGCGTCACAG TTTCTCAATC TTACACTGTG 
AGAGGTTTGT TGATAAACAT GATGGAGCAA TTCTGTCGGA GTACTAAAGA CTCTCTTCTG 
CAGATGTTAC ACAAAATGGA TGACCAGTCA CTAATTATAG AAGTGAGGCA ATACCTGCAA 
CATAAGAGGT ATTTGATATT CTTTGATGAC GTATGGCAAG AAGATTTTGC GGACCAGGTT 
CAATTTGCCA TGCCAAATAA CAATAAAGGT AGTAGGATTA TCATCACCAC TAGAATGATG 
TATGTTGCAG ATTTTTTCAA AAAATCCTTT CTAACTCATA TTCACAACCT GCAACTTTTG 
CCTCCAAACA AAGCATGGGA ACTTTTCTGC AAGAGAGTAT TTAGATATGA GCTTGGTGGA 
CATTGTCCAT CAGAACTTGA GGCTGTGTCA AAAGAAATTG TTCAAAAGTG CAGGCAGCTA 
CCTCTAGCAA TTGTAGCTAT TGGTGGTTTA CTTTCAACAA AATCTAAAAC AATGATTGAA 
TGGCAAAAGG TGAGTGAAAA TCTGAGTCTG GAATTAGGAC GTAATGCCCA TTTAACGTGT 
CTAGTAAAGA TTCTATCACT TAGTTATGAT GGACTTCCTT ATTACTTGAA ACCATGCATT 
TTGTATTTTG GTTTATACCC AGAGGACTAT GCAATTAATC ATAGGAGATT AACTCGACAA 
TGGATAGCTG AAGGGTTTGT AAAATTGGAA GAGAGAAGAA CTCCAGAGCA AGTTGCAGAA 
GAGTACTTGT CTGAATTGAT TCAGAGAAGT TTGGTTCAGG TCTCAAATGT TGGCTTTGAA 
GGAAAAGTTC AAACATGTCA AGTCCATGAT TTAATGCGGG AATTGATCAT TAGAAAAATG 
AAGGATTTAA ATTTCGGTCA TTGCATGCAT GAAGATAGTG AATCAGTTGC ACTTGGAAGA 
ACTAGACGCC TATCTATAGC CACTAATCCC AACAATGTGT TCAGGAGCAC TAATAATTTA 
CAATTTCGGG CTATTTATGT GTTTGAAGAA GATGGATCAT TGGAGCATTT CATGGGTAAA 
GTTTGTTCGC AGTCAAGAAT TTTGAAGGTC CTTGACATTC AAGGGACGTC GTTGAATCAT 
ATCCCTAAAA ATTTGGGGAA TCTTTTTCAC TTGCGGTACA TTAACCTAAG GAATACAAAT 
GTAAAGGCTC TTCCTAAATC TATTGGTGAG TTACATAACT TAGAGACATT GGATTTAAGA 
GAAACACTAG TGCATGAGAT ACCTAGTGAA ATAAACAAGC TGACAAAGTT ACGGCATCTG 
CTTGCTTTTC ATCGCAACTA TGAACAAAAA TATTCTGCTT TGGGCTCTAC AACTGGCGTG 
CTGATTGAAA AAGGTATTAA AAACATGATT TCCCTAAAAA ACCTTTATTA TGTGGAGGTA 
GATCATGGGG GAGTAGATCT CATTGAAGAG ATGAAAATGC TAAGGCAGTT AAGGAAGTTA 
GGTTTAAAGC ATGTAAGAAG GGAACATGGG AATGCCATAA GTGCTGCAGT ACAAGAGATG 
CAACACCTTG AATCACTTAA TATCACTGCA ATAGAAGAGG ATGAAATTAT TGACTTAAAC 
TTTGCGTCAA CCCCACCAAA ACTTCAACGG CTCCATTTGA AAGCTAGACT AGAGAAGTTT 
CCTGATTGGA TTCCAAAATT TGAGTGTCTT GTTCAGATTT GTCTGGCTTT ATCCAAGCTG 
AAAGATGATC CGTTGCAGTC ACTGAAAAAT TTGCCAAATC TTCTGAAGCT CAATTTGTTG 
GAGAATGCCT TTGATGGTGA AATTTTGCAT TTTCAAAATG GAGGATTTCA AATATTGAAA 
GAACTGATTC TCAGTCACTT GAATAGACCA AATTCTATCC TTATAGAGAA AGGAGCTTTG 
CTCTCTCTTG AAAATCTTAA ATTGGAAAGG ATCCCCAAAC TAAAGGATGT GCCTTCTGGC 
ATCAAACACT TGGATAAGCT CAAAGTCATT GACTTAGTTG ATATGCCAGA TGAATTTGTT 
AAGAGCATTG ATCCAGACGG AGGACAAGAC CATTGGATTA TAAACCATGT TCCTATTGTG 
TTCATTCGTC AATGGTTTGG CCCAAAATAC TATGATTATG AGGTTCGCAC TATTAACTCA 
TCCACCAAAG AAGTCCTGAG AGATGAGAAT GCTAAGGTGT CTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC11413331 ( XM_024779532.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_024635300.1 Medicago truncatula disease resistance protein RPM1 (LOC11413331), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMe193250
Clone ID Related Accession (Same CDS sequence)	XM_003598360.2 , XM_024779532.1 , XM_024779534.1 , XM_003598360.3 , XM_024779533.1 , XM_024779535.1 , XM_024779536.1
Accession Version	XM_024779532.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	disease resistance protein RPM1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016409.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAA CTGCAGTATC GTTTGTCCTG GGGGAAGTGT TCCAGTTCCT AAAAGAAGAA 
ACAAATCTTC TGAGAAGTGT ACACAGAGAT TTTTCAGACA TCAAAGATGA ACTTGAGAGC 
ATTCAAGTCT TCCTCAAAGA TGCAGACAGA AGGGCTGCGG ATGAAGCAGA CACTAACGAT 
GGAATAAGAA CTTGGGTGAA GCAGCTGAGA GAAGCATCAT TTCGCATTGA AGATGTCATT 
GATGAATACC TTCGGCTAAT GCATAGGGCC AATCCTCCTA GATGCGGATC TTTAGTCGGC 
AAGATTGCAA GGCAAATCAA AACTTTGATT CCCCGTCATC ATATAGCATC TGAAATTCAG 
GACATTAAAT TATCAATTCG TGGAATCAAG GAAAGAAGTG AGAGGTATAA CTTTCAAATT 
TCACACGAAC AAGGAACTTC AAGCAGCATT AGCATAGGAG AGAGAGAAAA TGGAAAATGG 
CATGATCCTC GACTGTCTTC TCTTTTTATT GAAGAAACAG AAGTTGTTGG TATTGAAGTC 
CCAAAAGAAG AACTGTCTGG TTGGTTATTG GATGGTGAAG CTGAGCGCAC GGTGATATCC 
GTGGTAGGAA TGGGAGGCCT AGGAAAAACC ACTCTTGCCA AGCTAGTTTT TGAAAGCAAA 
AAGGTATCAG CACACTTTGA TTGTCGTGCT TGCGTCACAG TTTCTCAATC TTACACTGTG 
AGAGGTTTGT TGATAAACAT GATGGAGCAA TTCTGTCGGA GTACTAAAGA CTCTCTTCTG 
CAGATGTTAC ACAAAATGGA TGACCAGTCA CTAATTATAG AAGTGAGGCA ATACCTGCAA 
CATAAGAGGT ATTTGATATT CTTTGATGAC GTATGGCAAG AAGATTTTGC GGACCAGGTT 
CAATTTGCCA TGCCAAATAA CAATAAAGGT AGTAGGATTA TCATCACCAC TAGAATGATG 
TATGTTGCAG ATTTTTTCAA AAAATCCTTT CTAACTCATA TTCACAACCT GCAACTTTTG 
CCTCCAAACA AAGCATGGGA ACTTTTCTGC AAGAGAGTAT TTAGATATGA GCTTGGTGGA 
CATTGTCCAT CAGAACTTGA GGCTGTGTCA AAAGAAATTG TTCAAAAGTG CAGGCAGCTA 
CCTCTAGCAA TTGTAGCTAT TGGTGGTTTA CTTTCAACAA AATCTAAAAC AATGATTGAA 
TGGCAAAAGG TGAGTGAAAA TCTGAGTCTG GAATTAGGAC GTAATGCCCA TTTAACGTGT 
CTAGTAAAGA TTCTATCACT TAGTTATGAT GGACTTCCTT ATTACTTGAA ACCATGCATT 
TTGTATTTTG GTTTATACCC AGAGGACTAT GCAATTAATC ATAGGAGATT AACTCGACAA 
TGGATAGCTG AAGGGTTTGT AAAATTGGAA GAGAGAAGAA CTCCAGAGCA AGTTGCAGAA 
GAGTACTTGT CTGAATTGAT TCAGAGAAGT TTGGTTCAGG TCTCAAATGT TGGCTTTGAA 
GGAAAAGTTC AAACATGTCA AGTCCATGAT TTAATGCGGG AATTGATCAT TAGAAAAATG 
AAGGATTTAA ATTTCGGTCA TTGCATGCAT GAAGATAGTG AATCAGTTGC ACTTGGAAGA 
ACTAGACGCC TATCTATAGC CACTAATCCC AACAATGTGT TCAGGAGCAC TAATAATTTA 
CAATTTCGGG CTATTTATGT GTTTGAAGAA GATGGATCAT TGGAGCATTT CATGGGTAAA 
GTTTGTTCGC AGTCAAGAAT TTTGAAGGTC CTTGACATTC AAGGGACGTC GTTGAATCAT 
ATCCCTAAAA ATTTGGGGAA TCTTTTTCAC TTGCGGTACA TTAACCTAAG GAATACAAAT 
GTAAAGGCTC TTCCTAAATC TATTGGTGAG TTACATAACT TAGAGACATT GGATTTAAGA 
GAAACACTAG TGCATGAGAT ACCTAGTGAA ATAAACAAGC TGACAAAGTT ACGGCATCTG 
CTTGCTTTTC ATCGCAACTA TGAACAAAAA TATTCTGCTT TGGGCTCTAC AACTGGCGTG 
CTGATTGAAA AAGGTATTAA AAACATGATT TCCCTAAAAA ACCTTTATTA TGTGGAGGTA 
GATCATGGGG GAGTAGATCT CATTGAAGAG ATGAAAATGC TAAGGCAGTT AAGGAAGTTA 
GGTTTAAAGC ATGTAAGAAG GGAACATGGG AATGCCATAA GTGCTGCAGT ACAAGAGATG 
CAACACCTTG AATCACTTAA TATCACTGCA ATAGAAGAGG ATGAAATTAT TGACTTAAAC 
TTTGCGTCAA CCCCACCAAA ACTTCAACGG CTCCATTTGA AAGCTAGACT AGAGAAGTTT 
CCTGATTGGA TTCCAAAATT TGAGTGTCTT GTTCAGATTT GTCTGGCTTT ATCCAAGCTG 
AAAGATGATC CGTTGCAGTC ACTGAAAAAT TTGCCAAATC TTCTGAAGCT CAATTTGTTG 
GAGAATGCCT TTGATGGTGA AATTTTGCAT TTTCAAAATG GAGGATTTCA AATATTGAAA 
GAACTGATTC TCAGTCACTT GAATAGACCA AATTCTATCC TTATAGAGAA AGGAGCTTTG 
CTCTCTCTTG AAAATCTTAA ATTGGAAAGG ATCCCCAAAC TAAAGGATGT GCCTTCTGGC 
ATCAAACACT TGGATAAGCT CAAAGTCATT GACTTAGTTG ATATGCCAGA TGAATTTGTT 
AAGAGCATTG ATCCAGACGG AGGACAAGAC CATTGGATTA TAAACCATGT TCCTATTGTG 
TTCATTCGTC AATGGTTTGG CCCAAAATAC TATGATTATG AGGTTCGCAC TATTAACTCA 
TCCACCAAAG AAGTCCTGAG AGATGAGAAT GCTAAGGTGT CTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024635300.1
CDS	915..3782
Translation	MAETAVSFVLGEVFQFLKEETNLLRSVHRDFSDIKDELESIQVFLKDADRRAADEADTNDGIRTWVKQLREASFRIEDVIDEYLRLMHRANPPRCGSLVGKIARQIKTLIPRHHIASEIQDIKLSIRGIKERSERYNFQISHEQGTSSSISIGERENGKWHDPRLSSLFIEETEVVGIEVPKEELSGWLLDGEAERTVISVVGMGGLGKTTLAKLVFESKKVSAHFDCRACVTVSQSYTVRGLLINMMEQFCRSTKDSLLQMLHKMDDQSLIIEVRQYLQHKRYLIFFDDVWQEDFADQVQFAMPNNNKGSRIIITTRMMYVADFFKKSFLTHIHNLQLLPPNKAWELFCKRVFRYELGGHCPSELEAVSKEIVQKCRQLPLAIVAIGGLLSTKSKTMIEWQKVSENLSLELGRNAHLTCLVKILSLSYDGLPYYLKPCILYFGLYPEDYAINHRRLTRQWIAEGFVKLEERRTPEQVAEEYLSELIQRSLVQVSNVGFEGKVQTCQVHDLMRELIIRKMKDLNFGHCMHEDSESVALGRTRRLSIATNPNNVFRSTNNLQFRAIYVFEEDGSLEHFMGKVCSQSRILKVLDIQGTSLNHIPKNLGNLFHLRYINLRNTNVKALPKSIGELHNLETLDLRETLVHEIPSEINKLTKLRHLLAFHRNYEQKYSALGSTTGVLIEKGIKNMISLKNLYYVEVDHGGVDLIEEMKMLRQLRKLGLKHVRREHGNAISAAVQEMQHLESLNITAIEEDEIIDLNFASTPPKLQRLHLKARLEKFPDWIPKFECLVQICLALSKLKDDPLQSLKNLPNLLKLNLLENAFDGEILHFQNGGFQILKELILSHLNRPNSILIEKGALLSLENLKLERIPKLKDVPSGIKHLDKLKVIDLVDMPDEFVKSIDPDGGQDHWIINHVPIVFIRQWFGPKYYDYEVRTINSSTKEVLRDENAKVSS

Target ORF information:

RefSeq Version	XM_024779532.1
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula disease resistance protein RPM1 (LOC11413331), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024779532.1

ORF Insert Sequence:

ATGGCAGAAA CTGCAGTATC GTTTGTCCTG GGGGAAGTGT TCCAGTTCCT AAAAGAAGAA 
ACAAATCTTC TGAGAAGTGT ACACAGAGAT TTTTCAGACA TCAAAGATGA ACTTGAGAGC 
ATTCAAGTCT TCCTCAAAGA TGCAGACAGA AGGGCTGCGG ATGAAGCAGA CACTAACGAT 
GGAATAAGAA CTTGGGTGAA GCAGCTGAGA GAAGCATCAT TTCGCATTGA AGATGTCATT 
GATGAATACC TTCGGCTAAT GCATAGGGCC AATCCTCCTA GATGCGGATC TTTAGTCGGC 
AAGATTGCAA GGCAAATCAA AACTTTGATT CCCCGTCATC ATATAGCATC TGAAATTCAG 
GACATTAAAT TATCAATTCG TGGAATCAAG GAAAGAAGTG AGAGGTATAA CTTTCAAATT 
TCACACGAAC AAGGAACTTC AAGCAGCATT AGCATAGGAG AGAGAGAAAA TGGAAAATGG 
CATGATCCTC GACTGTCTTC TCTTTTTATT GAAGAAACAG AAGTTGTTGG TATTGAAGTC 
CCAAAAGAAG AACTGTCTGG TTGGTTATTG GATGGTGAAG CTGAGCGCAC GGTGATATCC 
GTGGTAGGAA TGGGAGGCCT AGGAAAAACC ACTCTTGCCA AGCTAGTTTT TGAAAGCAAA 
AAGGTATCAG CACACTTTGA TTGTCGTGCT TGCGTCACAG TTTCTCAATC TTACACTGTG 
AGAGGTTTGT TGATAAACAT GATGGAGCAA TTCTGTCGGA GTACTAAAGA CTCTCTTCTG 
CAGATGTTAC ACAAAATGGA TGACCAGTCA CTAATTATAG AAGTGAGGCA ATACCTGCAA 
CATAAGAGGT ATTTGATATT CTTTGATGAC GTATGGCAAG AAGATTTTGC GGACCAGGTT 
CAATTTGCCA TGCCAAATAA CAATAAAGGT AGTAGGATTA TCATCACCAC TAGAATGATG 
TATGTTGCAG ATTTTTTCAA AAAATCCTTT CTAACTCATA TTCACAACCT GCAACTTTTG 
CCTCCAAACA AAGCATGGGA ACTTTTCTGC AAGAGAGTAT TTAGATATGA GCTTGGTGGA 
CATTGTCCAT CAGAACTTGA GGCTGTGTCA AAAGAAATTG TTCAAAAGTG CAGGCAGCTA 
CCTCTAGCAA TTGTAGCTAT TGGTGGTTTA CTTTCAACAA AATCTAAAAC AATGATTGAA 
TGGCAAAAGG TGAGTGAAAA TCTGAGTCTG GAATTAGGAC GTAATGCCCA TTTAACGTGT 
CTAGTAAAGA TTCTATCACT TAGTTATGAT GGACTTCCTT ATTACTTGAA ACCATGCATT 
TTGTATTTTG GTTTATACCC AGAGGACTAT GCAATTAATC ATAGGAGATT AACTCGACAA 
TGGATAGCTG AAGGGTTTGT AAAATTGGAA GAGAGAAGAA CTCCAGAGCA AGTTGCAGAA 
GAGTACTTGT CTGAATTGAT TCAGAGAAGT TTGGTTCAGG TCTCAAATGT TGGCTTTGAA 
GGAAAAGTTC AAACATGTCA AGTCCATGAT TTAATGCGGG AATTGATCAT TAGAAAAATG 
AAGGATTTAA ATTTCGGTCA TTGCATGCAT GAAGATAGTG AATCAGTTGC ACTTGGAAGA 
ACTAGACGCC TATCTATAGC CACTAATCCC AACAATGTGT TCAGGAGCAC TAATAATTTA 
CAATTTCGGG CTATTTATGT GTTTGAAGAA GATGGATCAT TGGAGCATTT CATGGGTAAA 
GTTTGTTCGC AGTCAAGAAT TTTGAAGGTC CTTGACATTC AAGGGACGTC GTTGAATCAT 
ATCCCTAAAA ATTTGGGGAA TCTTTTTCAC TTGCGGTACA TTAACCTAAG GAATACAAAT 
GTAAAGGCTC TTCCTAAATC TATTGGTGAG TTACATAACT TAGAGACATT GGATTTAAGA 
GAAACACTAG TGCATGAGAT ACCTAGTGAA ATAAACAAGC TGACAAAGTT ACGGCATCTG 
CTTGCTTTTC ATCGCAACTA TGAACAAAAA TATTCTGCTT TGGGCTCTAC AACTGGCGTG 
CTGATTGAAA AAGGTATTAA AAACATGATT TCCCTAAAAA ACCTTTATTA TGTGGAGGTA 
GATCATGGGG GAGTAGATCT CATTGAAGAG ATGAAAATGC TAAGGCAGTT AAGGAAGTTA 
GGTTTAAAGC ATGTAAGAAG GGAACATGGG AATGCCATAA GTGCTGCAGT ACAAGAGATG 
CAACACCTTG AATCACTTAA TATCACTGCA ATAGAAGAGG ATGAAATTAT TGACTTAAAC 
TTTGCGTCAA CCCCACCAAA ACTTCAACGG CTCCATTTGA AAGCTAGACT AGAGAAGTTT 
CCTGATTGGA TTCCAAAATT TGAGTGTCTT GTTCAGATTT GTCTGGCTTT ATCCAAGCTG 
AAAGATGATC CGTTGCAGTC ACTGAAAAAT TTGCCAAATC TTCTGAAGCT CAATTTGTTG 
GAGAATGCCT TTGATGGTGA AATTTTGCAT TTTCAAAATG GAGGATTTCA AATATTGAAA 
GAACTGATTC TCAGTCACTT GAATAGACCA AATTCTATCC TTATAGAGAA AGGAGCTTTG 
CTCTCTCTTG AAAATCTTAA ATTGGAAAGG ATCCCCAAAC TAAAGGATGT GCCTTCTGGC 
ATCAAACACT TGGATAAGCT CAAAGTCATT GACTTAGTTG ATATGCCAGA TGAATTTGTT 
AAGAGCATTG ATCCAGACGG AGGACAAGAC CATTGGATTA TAAACCATGT TCCTATTGTG 
TTCATTCGTC AATGGTTTGG CCCAAAATAC TATGATTATG AGGTTCGCAC TATTAACTCA 
TCCACCAAAG AAGTCCTGAG AGATGAGAAT GCTAAGGTGT CTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC11413331 ( XM_024779534.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_024635302.1 Medicago truncatula disease resistance protein RPM1 (LOC11413331), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMe193250
Clone ID Related Accession (Same CDS sequence)	XM_003598360.2 , XM_024779532.1 , XM_024779534.1 , XM_003598360.3 , XM_024779533.1 , XM_024779535.1 , XM_024779536.1
Accession Version	XM_024779534.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	disease resistance protein RPM1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016409.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAA CTGCAGTATC GTTTGTCCTG GGGGAAGTGT TCCAGTTCCT AAAAGAAGAA 
ACAAATCTTC TGAGAAGTGT ACACAGAGAT TTTTCAGACA TCAAAGATGA ACTTGAGAGC 
ATTCAAGTCT TCCTCAAAGA TGCAGACAGA AGGGCTGCGG ATGAAGCAGA CACTAACGAT 
GGAATAAGAA CTTGGGTGAA GCAGCTGAGA GAAGCATCAT TTCGCATTGA AGATGTCATT 
GATGAATACC TTCGGCTAAT GCATAGGGCC AATCCTCCTA GATGCGGATC TTTAGTCGGC 
AAGATTGCAA GGCAAATCAA AACTTTGATT CCCCGTCATC ATATAGCATC TGAAATTCAG 
GACATTAAAT TATCAATTCG TGGAATCAAG GAAAGAAGTG AGAGGTATAA CTTTCAAATT 
TCACACGAAC AAGGAACTTC AAGCAGCATT AGCATAGGAG AGAGAGAAAA TGGAAAATGG 
CATGATCCTC GACTGTCTTC TCTTTTTATT GAAGAAACAG AAGTTGTTGG TATTGAAGTC 
CCAAAAGAAG AACTGTCTGG TTGGTTATTG GATGGTGAAG CTGAGCGCAC GGTGATATCC 
GTGGTAGGAA TGGGAGGCCT AGGAAAAACC ACTCTTGCCA AGCTAGTTTT TGAAAGCAAA 
AAGGTATCAG CACACTTTGA TTGTCGTGCT TGCGTCACAG TTTCTCAATC TTACACTGTG 
AGAGGTTTGT TGATAAACAT GATGGAGCAA TTCTGTCGGA GTACTAAAGA CTCTCTTCTG 
CAGATGTTAC ACAAAATGGA TGACCAGTCA CTAATTATAG AAGTGAGGCA ATACCTGCAA 
CATAAGAGGT ATTTGATATT CTTTGATGAC GTATGGCAAG AAGATTTTGC GGACCAGGTT 
CAATTTGCCA TGCCAAATAA CAATAAAGGT AGTAGGATTA TCATCACCAC TAGAATGATG 
TATGTTGCAG ATTTTTTCAA AAAATCCTTT CTAACTCATA TTCACAACCT GCAACTTTTG 
CCTCCAAACA AAGCATGGGA ACTTTTCTGC AAGAGAGTAT TTAGATATGA GCTTGGTGGA 
CATTGTCCAT CAGAACTTGA GGCTGTGTCA AAAGAAATTG TTCAAAAGTG CAGGCAGCTA 
CCTCTAGCAA TTGTAGCTAT TGGTGGTTTA CTTTCAACAA AATCTAAAAC AATGATTGAA 
TGGCAAAAGG TGAGTGAAAA TCTGAGTCTG GAATTAGGAC GTAATGCCCA TTTAACGTGT 
CTAGTAAAGA TTCTATCACT TAGTTATGAT GGACTTCCTT ATTACTTGAA ACCATGCATT 
TTGTATTTTG GTTTATACCC AGAGGACTAT GCAATTAATC ATAGGAGATT AACTCGACAA 
TGGATAGCTG AAGGGTTTGT AAAATTGGAA GAGAGAAGAA CTCCAGAGCA AGTTGCAGAA 
GAGTACTTGT CTGAATTGAT TCAGAGAAGT TTGGTTCAGG TCTCAAATGT TGGCTTTGAA 
GGAAAAGTTC AAACATGTCA AGTCCATGAT TTAATGCGGG AATTGATCAT TAGAAAAATG 
AAGGATTTAA ATTTCGGTCA TTGCATGCAT GAAGATAGTG AATCAGTTGC ACTTGGAAGA 
ACTAGACGCC TATCTATAGC CACTAATCCC AACAATGTGT TCAGGAGCAC TAATAATTTA 
CAATTTCGGG CTATTTATGT GTTTGAAGAA GATGGATCAT TGGAGCATTT CATGGGTAAA 
GTTTGTTCGC AGTCAAGAAT TTTGAAGGTC CTTGACATTC AAGGGACGTC GTTGAATCAT 
ATCCCTAAAA ATTTGGGGAA TCTTTTTCAC TTGCGGTACA TTAACCTAAG GAATACAAAT 
GTAAAGGCTC TTCCTAAATC TATTGGTGAG TTACATAACT TAGAGACATT GGATTTAAGA 
GAAACACTAG TGCATGAGAT ACCTAGTGAA ATAAACAAGC TGACAAAGTT ACGGCATCTG 
CTTGCTTTTC ATCGCAACTA TGAACAAAAA TATTCTGCTT TGGGCTCTAC AACTGGCGTG 
CTGATTGAAA AAGGTATTAA AAACATGATT TCCCTAAAAA ACCTTTATTA TGTGGAGGTA 
GATCATGGGG GAGTAGATCT CATTGAAGAG ATGAAAATGC TAAGGCAGTT AAGGAAGTTA 
GGTTTAAAGC ATGTAAGAAG GGAACATGGG AATGCCATAA GTGCTGCAGT ACAAGAGATG 
CAACACCTTG AATCACTTAA TATCACTGCA ATAGAAGAGG ATGAAATTAT TGACTTAAAC 
TTTGCGTCAA CCCCACCAAA ACTTCAACGG CTCCATTTGA AAGCTAGACT AGAGAAGTTT 
CCTGATTGGA TTCCAAAATT TGAGTGTCTT GTTCAGATTT GTCTGGCTTT ATCCAAGCTG 
AAAGATGATC CGTTGCAGTC ACTGAAAAAT TTGCCAAATC TTCTGAAGCT CAATTTGTTG 
GAGAATGCCT TTGATGGTGA AATTTTGCAT TTTCAAAATG GAGGATTTCA AATATTGAAA 
GAACTGATTC TCAGTCACTT GAATAGACCA AATTCTATCC TTATAGAGAA AGGAGCTTTG 
CTCTCTCTTG AAAATCTTAA ATTGGAAAGG ATCCCCAAAC TAAAGGATGT GCCTTCTGGC 
ATCAAACACT TGGATAAGCT CAAAGTCATT GACTTAGTTG ATATGCCAGA TGAATTTGTT 
AAGAGCATTG ATCCAGACGG AGGACAAGAC CATTGGATTA TAAACCATGT TCCTATTGTG 
TTCATTCGTC AATGGTTTGG CCCAAAATAC TATGATTATG AGGTTCGCAC TATTAACTCA 
TCCACCAAAG AAGTCCTGAG AGATGAGAAT GCTAAGGTGT CTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024635302.1
CDS	596..3463
Translation	MAETAVSFVLGEVFQFLKEETNLLRSVHRDFSDIKDELESIQVFLKDADRRAADEADTNDGIRTWVKQLREASFRIEDVIDEYLRLMHRANPPRCGSLVGKIARQIKTLIPRHHIASEIQDIKLSIRGIKERSERYNFQISHEQGTSSSISIGERENGKWHDPRLSSLFIEETEVVGIEVPKEELSGWLLDGEAERTVISVVGMGGLGKTTLAKLVFESKKVSAHFDCRACVTVSQSYTVRGLLINMMEQFCRSTKDSLLQMLHKMDDQSLIIEVRQYLQHKRYLIFFDDVWQEDFADQVQFAMPNNNKGSRIIITTRMMYVADFFKKSFLTHIHNLQLLPPNKAWELFCKRVFRYELGGHCPSELEAVSKEIVQKCRQLPLAIVAIGGLLSTKSKTMIEWQKVSENLSLELGRNAHLTCLVKILSLSYDGLPYYLKPCILYFGLYPEDYAINHRRLTRQWIAEGFVKLEERRTPEQVAEEYLSELIQRSLVQVSNVGFEGKVQTCQVHDLMRELIIRKMKDLNFGHCMHEDSESVALGRTRRLSIATNPNNVFRSTNNLQFRAIYVFEEDGSLEHFMGKVCSQSRILKVLDIQGTSLNHIPKNLGNLFHLRYINLRNTNVKALPKSIGELHNLETLDLRETLVHEIPSEINKLTKLRHLLAFHRNYEQKYSALGSTTGVLIEKGIKNMISLKNLYYVEVDHGGVDLIEEMKMLRQLRKLGLKHVRREHGNAISAAVQEMQHLESLNITAIEEDEIIDLNFASTPPKLQRLHLKARLEKFPDWIPKFECLVQICLALSKLKDDPLQSLKNLPNLLKLNLLENAFDGEILHFQNGGFQILKELILSHLNRPNSILIEKGALLSLENLKLERIPKLKDVPSGIKHLDKLKVIDLVDMPDEFVKSIDPDGGQDHWIINHVPIVFIRQWFGPKYYDYEVRTINSSTKEVLRDENAKVSS

Target ORF information:

RefSeq Version	XM_024779534.1
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula disease resistance protein RPM1 (LOC11413331), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024779534.1

ORF Insert Sequence:

ATGGCAGAAA CTGCAGTATC GTTTGTCCTG GGGGAAGTGT TCCAGTTCCT AAAAGAAGAA 
ACAAATCTTC TGAGAAGTGT ACACAGAGAT TTTTCAGACA TCAAAGATGA ACTTGAGAGC 
ATTCAAGTCT TCCTCAAAGA TGCAGACAGA AGGGCTGCGG ATGAAGCAGA CACTAACGAT 
GGAATAAGAA CTTGGGTGAA GCAGCTGAGA GAAGCATCAT TTCGCATTGA AGATGTCATT 
GATGAATACC TTCGGCTAAT GCATAGGGCC AATCCTCCTA GATGCGGATC TTTAGTCGGC 
AAGATTGCAA GGCAAATCAA AACTTTGATT CCCCGTCATC ATATAGCATC TGAAATTCAG 
GACATTAAAT TATCAATTCG TGGAATCAAG GAAAGAAGTG AGAGGTATAA CTTTCAAATT 
TCACACGAAC AAGGAACTTC AAGCAGCATT AGCATAGGAG AGAGAGAAAA TGGAAAATGG 
CATGATCCTC GACTGTCTTC TCTTTTTATT GAAGAAACAG AAGTTGTTGG TATTGAAGTC 
CCAAAAGAAG AACTGTCTGG TTGGTTATTG GATGGTGAAG CTGAGCGCAC GGTGATATCC 
GTGGTAGGAA TGGGAGGCCT AGGAAAAACC ACTCTTGCCA AGCTAGTTTT TGAAAGCAAA 
AAGGTATCAG CACACTTTGA TTGTCGTGCT TGCGTCACAG TTTCTCAATC TTACACTGTG 
AGAGGTTTGT TGATAAACAT GATGGAGCAA TTCTGTCGGA GTACTAAAGA CTCTCTTCTG 
CAGATGTTAC ACAAAATGGA TGACCAGTCA CTAATTATAG AAGTGAGGCA ATACCTGCAA 
CATAAGAGGT ATTTGATATT CTTTGATGAC GTATGGCAAG AAGATTTTGC GGACCAGGTT 
CAATTTGCCA TGCCAAATAA CAATAAAGGT AGTAGGATTA TCATCACCAC TAGAATGATG 
TATGTTGCAG ATTTTTTCAA AAAATCCTTT CTAACTCATA TTCACAACCT GCAACTTTTG 
CCTCCAAACA AAGCATGGGA ACTTTTCTGC AAGAGAGTAT TTAGATATGA GCTTGGTGGA 
CATTGTCCAT CAGAACTTGA GGCTGTGTCA AAAGAAATTG TTCAAAAGTG CAGGCAGCTA 
CCTCTAGCAA TTGTAGCTAT TGGTGGTTTA CTTTCAACAA AATCTAAAAC AATGATTGAA 
TGGCAAAAGG TGAGTGAAAA TCTGAGTCTG GAATTAGGAC GTAATGCCCA TTTAACGTGT 
CTAGTAAAGA TTCTATCACT TAGTTATGAT GGACTTCCTT ATTACTTGAA ACCATGCATT 
TTGTATTTTG GTTTATACCC AGAGGACTAT GCAATTAATC ATAGGAGATT AACTCGACAA 
TGGATAGCTG AAGGGTTTGT AAAATTGGAA GAGAGAAGAA CTCCAGAGCA AGTTGCAGAA 
GAGTACTTGT CTGAATTGAT TCAGAGAAGT TTGGTTCAGG TCTCAAATGT TGGCTTTGAA 
GGAAAAGTTC AAACATGTCA AGTCCATGAT TTAATGCGGG AATTGATCAT TAGAAAAATG 
AAGGATTTAA ATTTCGGTCA TTGCATGCAT GAAGATAGTG AATCAGTTGC ACTTGGAAGA 
ACTAGACGCC TATCTATAGC CACTAATCCC AACAATGTGT TCAGGAGCAC TAATAATTTA 
CAATTTCGGG CTATTTATGT GTTTGAAGAA GATGGATCAT TGGAGCATTT CATGGGTAAA 
GTTTGTTCGC AGTCAAGAAT TTTGAAGGTC CTTGACATTC AAGGGACGTC GTTGAATCAT 
ATCCCTAAAA ATTTGGGGAA TCTTTTTCAC TTGCGGTACA TTAACCTAAG GAATACAAAT 
GTAAAGGCTC TTCCTAAATC TATTGGTGAG TTACATAACT TAGAGACATT GGATTTAAGA 
GAAACACTAG TGCATGAGAT ACCTAGTGAA ATAAACAAGC TGACAAAGTT ACGGCATCTG 
CTTGCTTTTC ATCGCAACTA TGAACAAAAA TATTCTGCTT TGGGCTCTAC AACTGGCGTG 
CTGATTGAAA AAGGTATTAA AAACATGATT TCCCTAAAAA ACCTTTATTA TGTGGAGGTA 
GATCATGGGG GAGTAGATCT CATTGAAGAG ATGAAAATGC TAAGGCAGTT AAGGAAGTTA 
GGTTTAAAGC ATGTAAGAAG GGAACATGGG AATGCCATAA GTGCTGCAGT ACAAGAGATG 
CAACACCTTG AATCACTTAA TATCACTGCA ATAGAAGAGG ATGAAATTAT TGACTTAAAC 
TTTGCGTCAA CCCCACCAAA ACTTCAACGG CTCCATTTGA AAGCTAGACT AGAGAAGTTT 
CCTGATTGGA TTCCAAAATT TGAGTGTCTT GTTCAGATTT GTCTGGCTTT ATCCAAGCTG 
AAAGATGATC CGTTGCAGTC ACTGAAAAAT TTGCCAAATC TTCTGAAGCT CAATTTGTTG 
GAGAATGCCT TTGATGGTGA AATTTTGCAT TTTCAAAATG GAGGATTTCA AATATTGAAA 
GAACTGATTC TCAGTCACTT GAATAGACCA AATTCTATCC TTATAGAGAA AGGAGCTTTG 
CTCTCTCTTG AAAATCTTAA ATTGGAAAGG ATCCCCAAAC TAAAGGATGT GCCTTCTGGC 
ATCAAACACT TGGATAAGCT CAAAGTCATT GACTTAGTTG ATATGCCAGA TGAATTTGTT 
AAGAGCATTG ATCCAGACGG AGGACAAGAC CATTGGATTA TAAACCATGT TCCTATTGTG 
TTCATTCGTC AATGGTTTGG CCCAAAATAC TATGATTATG AGGTTCGCAC TATTAACTCA 
TCCACCAAAG AAGTCCTGAG AGATGAGAAT GCTAAGGTGT CTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC11413331 ( XM_003598360.3 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_003598408.2 Medicago truncatula disease resistance protein RPM1 (LOC11413331), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMe193250
Clone ID Related Accession (Same CDS sequence)	XM_003598360.2 , XM_024779532.1 , XM_024779534.1 , XM_003598360.3 , XM_024779533.1 , XM_024779535.1 , XM_024779536.1
Accession Version	XM_003598360.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	disease resistance protein RPM1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016409.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2018 this sequence version replaced XM_003598360.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAA CTGCAGTATC GTTTGTCCTG GGGGAAGTGT TCCAGTTCCT AAAAGAAGAA 
ACAAATCTTC TGAGAAGTGT ACACAGAGAT TTTTCAGACA TCAAAGATGA ACTTGAGAGC 
ATTCAAGTCT TCCTCAAAGA TGCAGACAGA AGGGCTGCGG ATGAAGCAGA CACTAACGAT 
GGAATAAGAA CTTGGGTGAA GCAGCTGAGA GAAGCATCAT TTCGCATTGA AGATGTCATT 
GATGAATACC TTCGGCTAAT GCATAGGGCC AATCCTCCTA GATGCGGATC TTTAGTCGGC 
AAGATTGCAA GGCAAATCAA AACTTTGATT CCCCGTCATC ATATAGCATC TGAAATTCAG 
GACATTAAAT TATCAATTCG TGGAATCAAG GAAAGAAGTG AGAGGTATAA CTTTCAAATT 
TCACACGAAC AAGGAACTTC AAGCAGCATT AGCATAGGAG AGAGAGAAAA TGGAAAATGG 
CATGATCCTC GACTGTCTTC TCTTTTTATT GAAGAAACAG AAGTTGTTGG TATTGAAGTC 
CCAAAAGAAG AACTGTCTGG TTGGTTATTG GATGGTGAAG CTGAGCGCAC GGTGATATCC 
GTGGTAGGAA TGGGAGGCCT AGGAAAAACC ACTCTTGCCA AGCTAGTTTT TGAAAGCAAA 
AAGGTATCAG CACACTTTGA TTGTCGTGCT TGCGTCACAG TTTCTCAATC TTACACTGTG 
AGAGGTTTGT TGATAAACAT GATGGAGCAA TTCTGTCGGA GTACTAAAGA CTCTCTTCTG 
CAGATGTTAC ACAAAATGGA TGACCAGTCA CTAATTATAG AAGTGAGGCA ATACCTGCAA 
CATAAGAGGT ATTTGATATT CTTTGATGAC GTATGGCAAG AAGATTTTGC GGACCAGGTT 
CAATTTGCCA TGCCAAATAA CAATAAAGGT AGTAGGATTA TCATCACCAC TAGAATGATG 
TATGTTGCAG ATTTTTTCAA AAAATCCTTT CTAACTCATA TTCACAACCT GCAACTTTTG 
CCTCCAAACA AAGCATGGGA ACTTTTCTGC AAGAGAGTAT TTAGATATGA GCTTGGTGGA 
CATTGTCCAT CAGAACTTGA GGCTGTGTCA AAAGAAATTG TTCAAAAGTG CAGGCAGCTA 
CCTCTAGCAA TTGTAGCTAT TGGTGGTTTA CTTTCAACAA AATCTAAAAC AATGATTGAA 
TGGCAAAAGG TGAGTGAAAA TCTGAGTCTG GAATTAGGAC GTAATGCCCA TTTAACGTGT 
CTAGTAAAGA TTCTATCACT TAGTTATGAT GGACTTCCTT ATTACTTGAA ACCATGCATT 
TTGTATTTTG GTTTATACCC AGAGGACTAT GCAATTAATC ATAGGAGATT AACTCGACAA 
TGGATAGCTG AAGGGTTTGT AAAATTGGAA GAGAGAAGAA CTCCAGAGCA AGTTGCAGAA 
GAGTACTTGT CTGAATTGAT TCAGAGAAGT TTGGTTCAGG TCTCAAATGT TGGCTTTGAA 
GGAAAAGTTC AAACATGTCA AGTCCATGAT TTAATGCGGG AATTGATCAT TAGAAAAATG 
AAGGATTTAA ATTTCGGTCA TTGCATGCAT GAAGATAGTG AATCAGTTGC ACTTGGAAGA 
ACTAGACGCC TATCTATAGC CACTAATCCC AACAATGTGT TCAGGAGCAC TAATAATTTA 
CAATTTCGGG CTATTTATGT GTTTGAAGAA GATGGATCAT TGGAGCATTT CATGGGTAAA 
GTTTGTTCGC AGTCAAGAAT TTTGAAGGTC CTTGACATTC AAGGGACGTC GTTGAATCAT 
ATCCCTAAAA ATTTGGGGAA TCTTTTTCAC TTGCGGTACA TTAACCTAAG GAATACAAAT 
GTAAAGGCTC TTCCTAAATC TATTGGTGAG TTACATAACT TAGAGACATT GGATTTAAGA 
GAAACACTAG TGCATGAGAT ACCTAGTGAA ATAAACAAGC TGACAAAGTT ACGGCATCTG 
CTTGCTTTTC ATCGCAACTA TGAACAAAAA TATTCTGCTT TGGGCTCTAC AACTGGCGTG 
CTGATTGAAA AAGGTATTAA AAACATGATT TCCCTAAAAA ACCTTTATTA TGTGGAGGTA 
GATCATGGGG GAGTAGATCT CATTGAAGAG ATGAAAATGC TAAGGCAGTT AAGGAAGTTA 
GGTTTAAAGC ATGTAAGAAG GGAACATGGG AATGCCATAA GTGCTGCAGT ACAAGAGATG 
CAACACCTTG AATCACTTAA TATCACTGCA ATAGAAGAGG ATGAAATTAT TGACTTAAAC 
TTTGCGTCAA CCCCACCAAA ACTTCAACGG CTCCATTTGA AAGCTAGACT AGAGAAGTTT 
CCTGATTGGA TTCCAAAATT TGAGTGTCTT GTTCAGATTT GTCTGGCTTT ATCCAAGCTG 
AAAGATGATC CGTTGCAGTC ACTGAAAAAT TTGCCAAATC TTCTGAAGCT CAATTTGTTG 
GAGAATGCCT TTGATGGTGA AATTTTGCAT TTTCAAAATG GAGGATTTCA AATATTGAAA 
GAACTGATTC TCAGTCACTT GAATAGACCA AATTCTATCC TTATAGAGAA AGGAGCTTTG 
CTCTCTCTTG AAAATCTTAA ATTGGAAAGG ATCCCCAAAC TAAAGGATGT GCCTTCTGGC 
ATCAAACACT TGGATAAGCT CAAAGTCATT GACTTAGTTG ATATGCCAGA TGAATTTGTT 
AAGAGCATTG ATCCAGACGG AGGACAAGAC CATTGGATTA TAAACCATGT TCCTATTGTG 
TTCATTCGTC AATGGTTTGG CCCAAAATAC TATGATTATG AGGTTCGCAC TATTAACTCA 
TCCACCAAAG AAGTCCTGAG AGATGAGAAT GCTAAGGTGT CTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003598408.2
CDS	689..3556
Translation	MAETAVSFVLGEVFQFLKEETNLLRSVHRDFSDIKDELESIQVFLKDADRRAADEADTNDGIRTWVKQLREASFRIEDVIDEYLRLMHRANPPRCGSLVGKIARQIKTLIPRHHIASEIQDIKLSIRGIKERSERYNFQISHEQGTSSSISIGERENGKWHDPRLSSLFIEETEVVGIEVPKEELSGWLLDGEAERTVISVVGMGGLGKTTLAKLVFESKKVSAHFDCRACVTVSQSYTVRGLLINMMEQFCRSTKDSLLQMLHKMDDQSLIIEVRQYLQHKRYLIFFDDVWQEDFADQVQFAMPNNNKGSRIIITTRMMYVADFFKKSFLTHIHNLQLLPPNKAWELFCKRVFRYELGGHCPSELEAVSKEIVQKCRQLPLAIVAIGGLLSTKSKTMIEWQKVSENLSLELGRNAHLTCLVKILSLSYDGLPYYLKPCILYFGLYPEDYAINHRRLTRQWIAEGFVKLEERRTPEQVAEEYLSELIQRSLVQVSNVGFEGKVQTCQVHDLMRELIIRKMKDLNFGHCMHEDSESVALGRTRRLSIATNPNNVFRSTNNLQFRAIYVFEEDGSLEHFMGKVCSQSRILKVLDIQGTSLNHIPKNLGNLFHLRYINLRNTNVKALPKSIGELHNLETLDLRETLVHEIPSEINKLTKLRHLLAFHRNYEQKYSALGSTTGVLIEKGIKNMISLKNLYYVEVDHGGVDLIEEMKMLRQLRKLGLKHVRREHGNAISAAVQEMQHLESLNITAIEEDEIIDLNFASTPPKLQRLHLKARLEKFPDWIPKFECLVQICLALSKLKDDPLQSLKNLPNLLKLNLLENAFDGEILHFQNGGFQILKELILSHLNRPNSILIEKGALLSLENLKLERIPKLKDVPSGIKHLDKLKVIDLVDMPDEFVKSIDPDGGQDHWIINHVPIVFIRQWFGPKYYDYEVRTINSSTKEVLRDENAKVSS

Target ORF information:

RefSeq Version	XM_003598360.3
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula disease resistance protein RPM1 (LOC11413331), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003598360.3

ORF Insert Sequence:

ATGGCAGAAA CTGCAGTATC GTTTGTCCTG GGGGAAGTGT TCCAGTTCCT AAAAGAAGAA 
ACAAATCTTC TGAGAAGTGT ACACAGAGAT TTTTCAGACA TCAAAGATGA ACTTGAGAGC 
ATTCAAGTCT TCCTCAAAGA TGCAGACAGA AGGGCTGCGG ATGAAGCAGA CACTAACGAT 
GGAATAAGAA CTTGGGTGAA GCAGCTGAGA GAAGCATCAT TTCGCATTGA AGATGTCATT 
GATGAATACC TTCGGCTAAT GCATAGGGCC AATCCTCCTA GATGCGGATC TTTAGTCGGC 
AAGATTGCAA GGCAAATCAA AACTTTGATT CCCCGTCATC ATATAGCATC TGAAATTCAG 
GACATTAAAT TATCAATTCG TGGAATCAAG GAAAGAAGTG AGAGGTATAA CTTTCAAATT 
TCACACGAAC AAGGAACTTC AAGCAGCATT AGCATAGGAG AGAGAGAAAA TGGAAAATGG 
CATGATCCTC GACTGTCTTC TCTTTTTATT GAAGAAACAG AAGTTGTTGG TATTGAAGTC 
CCAAAAGAAG AACTGTCTGG TTGGTTATTG GATGGTGAAG CTGAGCGCAC GGTGATATCC 
GTGGTAGGAA TGGGAGGCCT AGGAAAAACC ACTCTTGCCA AGCTAGTTTT TGAAAGCAAA 
AAGGTATCAG CACACTTTGA TTGTCGTGCT TGCGTCACAG TTTCTCAATC TTACACTGTG 
AGAGGTTTGT TGATAAACAT GATGGAGCAA TTCTGTCGGA GTACTAAAGA CTCTCTTCTG 
CAGATGTTAC ACAAAATGGA TGACCAGTCA CTAATTATAG AAGTGAGGCA ATACCTGCAA 
CATAAGAGGT ATTTGATATT CTTTGATGAC GTATGGCAAG AAGATTTTGC GGACCAGGTT 
CAATTTGCCA TGCCAAATAA CAATAAAGGT AGTAGGATTA TCATCACCAC TAGAATGATG 
TATGTTGCAG ATTTTTTCAA AAAATCCTTT CTAACTCATA TTCACAACCT GCAACTTTTG 
CCTCCAAACA AAGCATGGGA ACTTTTCTGC AAGAGAGTAT TTAGATATGA GCTTGGTGGA 
CATTGTCCAT CAGAACTTGA GGCTGTGTCA AAAGAAATTG TTCAAAAGTG CAGGCAGCTA 
CCTCTAGCAA TTGTAGCTAT TGGTGGTTTA CTTTCAACAA AATCTAAAAC AATGATTGAA 
TGGCAAAAGG TGAGTGAAAA TCTGAGTCTG GAATTAGGAC GTAATGCCCA TTTAACGTGT 
CTAGTAAAGA TTCTATCACT TAGTTATGAT GGACTTCCTT ATTACTTGAA ACCATGCATT 
TTGTATTTTG GTTTATACCC AGAGGACTAT GCAATTAATC ATAGGAGATT AACTCGACAA 
TGGATAGCTG AAGGGTTTGT AAAATTGGAA GAGAGAAGAA CTCCAGAGCA AGTTGCAGAA 
GAGTACTTGT CTGAATTGAT TCAGAGAAGT TTGGTTCAGG TCTCAAATGT TGGCTTTGAA 
GGAAAAGTTC AAACATGTCA AGTCCATGAT TTAATGCGGG AATTGATCAT TAGAAAAATG 
AAGGATTTAA ATTTCGGTCA TTGCATGCAT GAAGATAGTG AATCAGTTGC ACTTGGAAGA 
ACTAGACGCC TATCTATAGC CACTAATCCC AACAATGTGT TCAGGAGCAC TAATAATTTA 
CAATTTCGGG CTATTTATGT GTTTGAAGAA GATGGATCAT TGGAGCATTT CATGGGTAAA 
GTTTGTTCGC AGTCAAGAAT TTTGAAGGTC CTTGACATTC AAGGGACGTC GTTGAATCAT 
ATCCCTAAAA ATTTGGGGAA TCTTTTTCAC TTGCGGTACA TTAACCTAAG GAATACAAAT 
GTAAAGGCTC TTCCTAAATC TATTGGTGAG TTACATAACT TAGAGACATT GGATTTAAGA 
GAAACACTAG TGCATGAGAT ACCTAGTGAA ATAAACAAGC TGACAAAGTT ACGGCATCTG 
CTTGCTTTTC ATCGCAACTA TGAACAAAAA TATTCTGCTT TGGGCTCTAC AACTGGCGTG 
CTGATTGAAA AAGGTATTAA AAACATGATT TCCCTAAAAA ACCTTTATTA TGTGGAGGTA 
GATCATGGGG GAGTAGATCT CATTGAAGAG ATGAAAATGC TAAGGCAGTT AAGGAAGTTA 
GGTTTAAAGC ATGTAAGAAG GGAACATGGG AATGCCATAA GTGCTGCAGT ACAAGAGATG 
CAACACCTTG AATCACTTAA TATCACTGCA ATAGAAGAGG ATGAAATTAT TGACTTAAAC 
TTTGCGTCAA CCCCACCAAA ACTTCAACGG CTCCATTTGA AAGCTAGACT AGAGAAGTTT 
CCTGATTGGA TTCCAAAATT TGAGTGTCTT GTTCAGATTT GTCTGGCTTT ATCCAAGCTG 
AAAGATGATC CGTTGCAGTC ACTGAAAAAT TTGCCAAATC TTCTGAAGCT CAATTTGTTG 
GAGAATGCCT TTGATGGTGA AATTTTGCAT TTTCAAAATG GAGGATTTCA AATATTGAAA 
GAACTGATTC TCAGTCACTT GAATAGACCA AATTCTATCC TTATAGAGAA AGGAGCTTTG 
CTCTCTCTTG AAAATCTTAA ATTGGAAAGG ATCCCCAAAC TAAAGGATGT GCCTTCTGGC 
ATCAAACACT TGGATAAGCT CAAAGTCATT GACTTAGTTG ATATGCCAGA TGAATTTGTT 
AAGAGCATTG ATCCAGACGG AGGACAAGAC CATTGGATTA TAAACCATGT TCCTATTGTG 
TTCATTCGTC AATGGTTTGG CCCAAAATAC TATGATTATG AGGTTCGCAC TATTAACTCA 
TCCACCAAAG AAGTCCTGAG AGATGAGAAT GCTAAGGTGT CTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC11413331 ( XM_024779533.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_024635301.1 Medicago truncatula disease resistance protein RPM1 (LOC11413331), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMe193250
Clone ID Related Accession (Same CDS sequence)	XM_003598360.2 , XM_024779532.1 , XM_024779534.1 , XM_003598360.3 , XM_024779533.1 , XM_024779535.1 , XM_024779536.1
Accession Version	XM_024779533.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	disease resistance protein RPM1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016409.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAA CTGCAGTATC GTTTGTCCTG GGGGAAGTGT TCCAGTTCCT AAAAGAAGAA 
ACAAATCTTC TGAGAAGTGT ACACAGAGAT TTTTCAGACA TCAAAGATGA ACTTGAGAGC 
ATTCAAGTCT TCCTCAAAGA TGCAGACAGA AGGGCTGCGG ATGAAGCAGA CACTAACGAT 
GGAATAAGAA CTTGGGTGAA GCAGCTGAGA GAAGCATCAT TTCGCATTGA AGATGTCATT 
GATGAATACC TTCGGCTAAT GCATAGGGCC AATCCTCCTA GATGCGGATC TTTAGTCGGC 
AAGATTGCAA GGCAAATCAA AACTTTGATT CCCCGTCATC ATATAGCATC TGAAATTCAG 
GACATTAAAT TATCAATTCG TGGAATCAAG GAAAGAAGTG AGAGGTATAA CTTTCAAATT 
TCACACGAAC AAGGAACTTC AAGCAGCATT AGCATAGGAG AGAGAGAAAA TGGAAAATGG 
CATGATCCTC GACTGTCTTC TCTTTTTATT GAAGAAACAG AAGTTGTTGG TATTGAAGTC 
CCAAAAGAAG AACTGTCTGG TTGGTTATTG GATGGTGAAG CTGAGCGCAC GGTGATATCC 
GTGGTAGGAA TGGGAGGCCT AGGAAAAACC ACTCTTGCCA AGCTAGTTTT TGAAAGCAAA 
AAGGTATCAG CACACTTTGA TTGTCGTGCT TGCGTCACAG TTTCTCAATC TTACACTGTG 
AGAGGTTTGT TGATAAACAT GATGGAGCAA TTCTGTCGGA GTACTAAAGA CTCTCTTCTG 
CAGATGTTAC ACAAAATGGA TGACCAGTCA CTAATTATAG AAGTGAGGCA ATACCTGCAA 
CATAAGAGGT ATTTGATATT CTTTGATGAC GTATGGCAAG AAGATTTTGC GGACCAGGTT 
CAATTTGCCA TGCCAAATAA CAATAAAGGT AGTAGGATTA TCATCACCAC TAGAATGATG 
TATGTTGCAG ATTTTTTCAA AAAATCCTTT CTAACTCATA TTCACAACCT GCAACTTTTG 
CCTCCAAACA AAGCATGGGA ACTTTTCTGC AAGAGAGTAT TTAGATATGA GCTTGGTGGA 
CATTGTCCAT CAGAACTTGA GGCTGTGTCA AAAGAAATTG TTCAAAAGTG CAGGCAGCTA 
CCTCTAGCAA TTGTAGCTAT TGGTGGTTTA CTTTCAACAA AATCTAAAAC AATGATTGAA 
TGGCAAAAGG TGAGTGAAAA TCTGAGTCTG GAATTAGGAC GTAATGCCCA TTTAACGTGT 
CTAGTAAAGA TTCTATCACT TAGTTATGAT GGACTTCCTT ATTACTTGAA ACCATGCATT 
TTGTATTTTG GTTTATACCC AGAGGACTAT GCAATTAATC ATAGGAGATT AACTCGACAA 
TGGATAGCTG AAGGGTTTGT AAAATTGGAA GAGAGAAGAA CTCCAGAGCA AGTTGCAGAA 
GAGTACTTGT CTGAATTGAT TCAGAGAAGT TTGGTTCAGG TCTCAAATGT TGGCTTTGAA 
GGAAAAGTTC AAACATGTCA AGTCCATGAT TTAATGCGGG AATTGATCAT TAGAAAAATG 
AAGGATTTAA ATTTCGGTCA TTGCATGCAT GAAGATAGTG AATCAGTTGC ACTTGGAAGA 
ACTAGACGCC TATCTATAGC CACTAATCCC AACAATGTGT TCAGGAGCAC TAATAATTTA 
CAATTTCGGG CTATTTATGT GTTTGAAGAA GATGGATCAT TGGAGCATTT CATGGGTAAA 
GTTTGTTCGC AGTCAAGAAT TTTGAAGGTC CTTGACATTC AAGGGACGTC GTTGAATCAT 
ATCCCTAAAA ATTTGGGGAA TCTTTTTCAC TTGCGGTACA TTAACCTAAG GAATACAAAT 
GTAAAGGCTC TTCCTAAATC TATTGGTGAG TTACATAACT TAGAGACATT GGATTTAAGA 
GAAACACTAG TGCATGAGAT ACCTAGTGAA ATAAACAAGC TGACAAAGTT ACGGCATCTG 
CTTGCTTTTC ATCGCAACTA TGAACAAAAA TATTCTGCTT TGGGCTCTAC AACTGGCGTG 
CTGATTGAAA AAGGTATTAA AAACATGATT TCCCTAAAAA ACCTTTATTA TGTGGAGGTA 
GATCATGGGG GAGTAGATCT CATTGAAGAG ATGAAAATGC TAAGGCAGTT AAGGAAGTTA 
GGTTTAAAGC ATGTAAGAAG GGAACATGGG AATGCCATAA GTGCTGCAGT ACAAGAGATG 
CAACACCTTG AATCACTTAA TATCACTGCA ATAGAAGAGG ATGAAATTAT TGACTTAAAC 
TTTGCGTCAA CCCCACCAAA ACTTCAACGG CTCCATTTGA AAGCTAGACT AGAGAAGTTT 
CCTGATTGGA TTCCAAAATT TGAGTGTCTT GTTCAGATTT GTCTGGCTTT ATCCAAGCTG 
AAAGATGATC CGTTGCAGTC ACTGAAAAAT TTGCCAAATC TTCTGAAGCT CAATTTGTTG 
GAGAATGCCT TTGATGGTGA AATTTTGCAT TTTCAAAATG GAGGATTTCA AATATTGAAA 
GAACTGATTC TCAGTCACTT GAATAGACCA AATTCTATCC TTATAGAGAA AGGAGCTTTG 
CTCTCTCTTG AAAATCTTAA ATTGGAAAGG ATCCCCAAAC TAAAGGATGT GCCTTCTGGC 
ATCAAACACT TGGATAAGCT CAAAGTCATT GACTTAGTTG ATATGCCAGA TGAATTTGTT 
AAGAGCATTG ATCCAGACGG AGGACAAGAC CATTGGATTA TAAACCATGT TCCTATTGTG 
TTCATTCGTC AATGGTTTGG CCCAAAATAC TATGATTATG AGGTTCGCAC TATTAACTCA 
TCCACCAAAG AAGTCCTGAG AGATGAGAAT GCTAAGGTGT CTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024635301.1
CDS	617..3484
Translation	MAETAVSFVLGEVFQFLKEETNLLRSVHRDFSDIKDELESIQVFLKDADRRAADEADTNDGIRTWVKQLREASFRIEDVIDEYLRLMHRANPPRCGSLVGKIARQIKTLIPRHHIASEIQDIKLSIRGIKERSERYNFQISHEQGTSSSISIGERENGKWHDPRLSSLFIEETEVVGIEVPKEELSGWLLDGEAERTVISVVGMGGLGKTTLAKLVFESKKVSAHFDCRACVTVSQSYTVRGLLINMMEQFCRSTKDSLLQMLHKMDDQSLIIEVRQYLQHKRYLIFFDDVWQEDFADQVQFAMPNNNKGSRIIITTRMMYVADFFKKSFLTHIHNLQLLPPNKAWELFCKRVFRYELGGHCPSELEAVSKEIVQKCRQLPLAIVAIGGLLSTKSKTMIEWQKVSENLSLELGRNAHLTCLVKILSLSYDGLPYYLKPCILYFGLYPEDYAINHRRLTRQWIAEGFVKLEERRTPEQVAEEYLSELIQRSLVQVSNVGFEGKVQTCQVHDLMRELIIRKMKDLNFGHCMHEDSESVALGRTRRLSIATNPNNVFRSTNNLQFRAIYVFEEDGSLEHFMGKVCSQSRILKVLDIQGTSLNHIPKNLGNLFHLRYINLRNTNVKALPKSIGELHNLETLDLRETLVHEIPSEINKLTKLRHLLAFHRNYEQKYSALGSTTGVLIEKGIKNMISLKNLYYVEVDHGGVDLIEEMKMLRQLRKLGLKHVRREHGNAISAAVQEMQHLESLNITAIEEDEIIDLNFASTPPKLQRLHLKARLEKFPDWIPKFECLVQICLALSKLKDDPLQSLKNLPNLLKLNLLENAFDGEILHFQNGGFQILKELILSHLNRPNSILIEKGALLSLENLKLERIPKLKDVPSGIKHLDKLKVIDLVDMPDEFVKSIDPDGGQDHWIINHVPIVFIRQWFGPKYYDYEVRTINSSTKEVLRDENAKVSS

Target ORF information:

RefSeq Version	XM_024779533.1
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula disease resistance protein RPM1 (LOC11413331), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024779533.1

ORF Insert Sequence:

ATGGCAGAAA CTGCAGTATC GTTTGTCCTG GGGGAAGTGT TCCAGTTCCT AAAAGAAGAA 
ACAAATCTTC TGAGAAGTGT ACACAGAGAT TTTTCAGACA TCAAAGATGA ACTTGAGAGC 
ATTCAAGTCT TCCTCAAAGA TGCAGACAGA AGGGCTGCGG ATGAAGCAGA CACTAACGAT 
GGAATAAGAA CTTGGGTGAA GCAGCTGAGA GAAGCATCAT TTCGCATTGA AGATGTCATT 
GATGAATACC TTCGGCTAAT GCATAGGGCC AATCCTCCTA GATGCGGATC TTTAGTCGGC 
AAGATTGCAA GGCAAATCAA AACTTTGATT CCCCGTCATC ATATAGCATC TGAAATTCAG 
GACATTAAAT TATCAATTCG TGGAATCAAG GAAAGAAGTG AGAGGTATAA CTTTCAAATT 
TCACACGAAC AAGGAACTTC AAGCAGCATT AGCATAGGAG AGAGAGAAAA TGGAAAATGG 
CATGATCCTC GACTGTCTTC TCTTTTTATT GAAGAAACAG AAGTTGTTGG TATTGAAGTC 
CCAAAAGAAG AACTGTCTGG TTGGTTATTG GATGGTGAAG CTGAGCGCAC GGTGATATCC 
GTGGTAGGAA TGGGAGGCCT AGGAAAAACC ACTCTTGCCA AGCTAGTTTT TGAAAGCAAA 
AAGGTATCAG CACACTTTGA TTGTCGTGCT TGCGTCACAG TTTCTCAATC TTACACTGTG 
AGAGGTTTGT TGATAAACAT GATGGAGCAA TTCTGTCGGA GTACTAAAGA CTCTCTTCTG 
CAGATGTTAC ACAAAATGGA TGACCAGTCA CTAATTATAG AAGTGAGGCA ATACCTGCAA 
CATAAGAGGT ATTTGATATT CTTTGATGAC GTATGGCAAG AAGATTTTGC GGACCAGGTT 
CAATTTGCCA TGCCAAATAA CAATAAAGGT AGTAGGATTA TCATCACCAC TAGAATGATG 
TATGTTGCAG ATTTTTTCAA AAAATCCTTT CTAACTCATA TTCACAACCT GCAACTTTTG 
CCTCCAAACA AAGCATGGGA ACTTTTCTGC AAGAGAGTAT TTAGATATGA GCTTGGTGGA 
CATTGTCCAT CAGAACTTGA GGCTGTGTCA AAAGAAATTG TTCAAAAGTG CAGGCAGCTA 
CCTCTAGCAA TTGTAGCTAT TGGTGGTTTA CTTTCAACAA AATCTAAAAC AATGATTGAA 
TGGCAAAAGG TGAGTGAAAA TCTGAGTCTG GAATTAGGAC GTAATGCCCA TTTAACGTGT 
CTAGTAAAGA TTCTATCACT TAGTTATGAT GGACTTCCTT ATTACTTGAA ACCATGCATT 
TTGTATTTTG GTTTATACCC AGAGGACTAT GCAATTAATC ATAGGAGATT AACTCGACAA 
TGGATAGCTG AAGGGTTTGT AAAATTGGAA GAGAGAAGAA CTCCAGAGCA AGTTGCAGAA 
GAGTACTTGT CTGAATTGAT TCAGAGAAGT TTGGTTCAGG TCTCAAATGT TGGCTTTGAA 
GGAAAAGTTC AAACATGTCA AGTCCATGAT TTAATGCGGG AATTGATCAT TAGAAAAATG 
AAGGATTTAA ATTTCGGTCA TTGCATGCAT GAAGATAGTG AATCAGTTGC ACTTGGAAGA 
ACTAGACGCC TATCTATAGC CACTAATCCC AACAATGTGT TCAGGAGCAC TAATAATTTA 
CAATTTCGGG CTATTTATGT GTTTGAAGAA GATGGATCAT TGGAGCATTT CATGGGTAAA 
GTTTGTTCGC AGTCAAGAAT TTTGAAGGTC CTTGACATTC AAGGGACGTC GTTGAATCAT 
ATCCCTAAAA ATTTGGGGAA TCTTTTTCAC TTGCGGTACA TTAACCTAAG GAATACAAAT 
GTAAAGGCTC TTCCTAAATC TATTGGTGAG TTACATAACT TAGAGACATT GGATTTAAGA 
GAAACACTAG TGCATGAGAT ACCTAGTGAA ATAAACAAGC TGACAAAGTT ACGGCATCTG 
CTTGCTTTTC ATCGCAACTA TGAACAAAAA TATTCTGCTT TGGGCTCTAC AACTGGCGTG 
CTGATTGAAA AAGGTATTAA AAACATGATT TCCCTAAAAA ACCTTTATTA TGTGGAGGTA 
GATCATGGGG GAGTAGATCT CATTGAAGAG ATGAAAATGC TAAGGCAGTT AAGGAAGTTA 
GGTTTAAAGC ATGTAAGAAG GGAACATGGG AATGCCATAA GTGCTGCAGT ACAAGAGATG 
CAACACCTTG AATCACTTAA TATCACTGCA ATAGAAGAGG ATGAAATTAT TGACTTAAAC 
TTTGCGTCAA CCCCACCAAA ACTTCAACGG CTCCATTTGA AAGCTAGACT AGAGAAGTTT 
CCTGATTGGA TTCCAAAATT TGAGTGTCTT GTTCAGATTT GTCTGGCTTT ATCCAAGCTG 
AAAGATGATC CGTTGCAGTC ACTGAAAAAT TTGCCAAATC TTCTGAAGCT CAATTTGTTG 
GAGAATGCCT TTGATGGTGA AATTTTGCAT TTTCAAAATG GAGGATTTCA AATATTGAAA 
GAACTGATTC TCAGTCACTT GAATAGACCA AATTCTATCC TTATAGAGAA AGGAGCTTTG 
CTCTCTCTTG AAAATCTTAA ATTGGAAAGG ATCCCCAAAC TAAAGGATGT GCCTTCTGGC 
ATCAAACACT TGGATAAGCT CAAAGTCATT GACTTAGTTG ATATGCCAGA TGAATTTGTT 
AAGAGCATTG ATCCAGACGG AGGACAAGAC CATTGGATTA TAAACCATGT TCCTATTGTG 
TTCATTCGTC AATGGTTTGG CCCAAAATAC TATGATTATG AGGTTCGCAC TATTAACTCA 
TCCACCAAAG AAGTCCTGAG AGATGAGAAT GCTAAGGTGT CTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC11413331 ( XM_024779535.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_024635303.1 Medicago truncatula disease resistance protein RPM1 (LOC11413331), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMe193250
Clone ID Related Accession (Same CDS sequence)	XM_003598360.2 , XM_024779532.1 , XM_024779534.1 , XM_003598360.3 , XM_024779533.1 , XM_024779535.1 , XM_024779536.1
Accession Version	XM_024779535.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	disease resistance protein RPM1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016409.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAA CTGCAGTATC GTTTGTCCTG GGGGAAGTGT TCCAGTTCCT AAAAGAAGAA 
ACAAATCTTC TGAGAAGTGT ACACAGAGAT TTTTCAGACA TCAAAGATGA ACTTGAGAGC 
ATTCAAGTCT TCCTCAAAGA TGCAGACAGA AGGGCTGCGG ATGAAGCAGA CACTAACGAT 
GGAATAAGAA CTTGGGTGAA GCAGCTGAGA GAAGCATCAT TTCGCATTGA AGATGTCATT 
GATGAATACC TTCGGCTAAT GCATAGGGCC AATCCTCCTA GATGCGGATC TTTAGTCGGC 
AAGATTGCAA GGCAAATCAA AACTTTGATT CCCCGTCATC ATATAGCATC TGAAATTCAG 
GACATTAAAT TATCAATTCG TGGAATCAAG GAAAGAAGTG AGAGGTATAA CTTTCAAATT 
TCACACGAAC AAGGAACTTC AAGCAGCATT AGCATAGGAG AGAGAGAAAA TGGAAAATGG 
CATGATCCTC GACTGTCTTC TCTTTTTATT GAAGAAACAG AAGTTGTTGG TATTGAAGTC 
CCAAAAGAAG AACTGTCTGG TTGGTTATTG GATGGTGAAG CTGAGCGCAC GGTGATATCC 
GTGGTAGGAA TGGGAGGCCT AGGAAAAACC ACTCTTGCCA AGCTAGTTTT TGAAAGCAAA 
AAGGTATCAG CACACTTTGA TTGTCGTGCT TGCGTCACAG TTTCTCAATC TTACACTGTG 
AGAGGTTTGT TGATAAACAT GATGGAGCAA TTCTGTCGGA GTACTAAAGA CTCTCTTCTG 
CAGATGTTAC ACAAAATGGA TGACCAGTCA CTAATTATAG AAGTGAGGCA ATACCTGCAA 
CATAAGAGGT ATTTGATATT CTTTGATGAC GTATGGCAAG AAGATTTTGC GGACCAGGTT 
CAATTTGCCA TGCCAAATAA CAATAAAGGT AGTAGGATTA TCATCACCAC TAGAATGATG 
TATGTTGCAG ATTTTTTCAA AAAATCCTTT CTAACTCATA TTCACAACCT GCAACTTTTG 
CCTCCAAACA AAGCATGGGA ACTTTTCTGC AAGAGAGTAT TTAGATATGA GCTTGGTGGA 
CATTGTCCAT CAGAACTTGA GGCTGTGTCA AAAGAAATTG TTCAAAAGTG CAGGCAGCTA 
CCTCTAGCAA TTGTAGCTAT TGGTGGTTTA CTTTCAACAA AATCTAAAAC AATGATTGAA 
TGGCAAAAGG TGAGTGAAAA TCTGAGTCTG GAATTAGGAC GTAATGCCCA TTTAACGTGT 
CTAGTAAAGA TTCTATCACT TAGTTATGAT GGACTTCCTT ATTACTTGAA ACCATGCATT 
TTGTATTTTG GTTTATACCC AGAGGACTAT GCAATTAATC ATAGGAGATT AACTCGACAA 
TGGATAGCTG AAGGGTTTGT AAAATTGGAA GAGAGAAGAA CTCCAGAGCA AGTTGCAGAA 
GAGTACTTGT CTGAATTGAT TCAGAGAAGT TTGGTTCAGG TCTCAAATGT TGGCTTTGAA 
GGAAAAGTTC AAACATGTCA AGTCCATGAT TTAATGCGGG AATTGATCAT TAGAAAAATG 
AAGGATTTAA ATTTCGGTCA TTGCATGCAT GAAGATAGTG AATCAGTTGC ACTTGGAAGA 
ACTAGACGCC TATCTATAGC CACTAATCCC AACAATGTGT TCAGGAGCAC TAATAATTTA 
CAATTTCGGG CTATTTATGT GTTTGAAGAA GATGGATCAT TGGAGCATTT CATGGGTAAA 
GTTTGTTCGC AGTCAAGAAT TTTGAAGGTC CTTGACATTC AAGGGACGTC GTTGAATCAT 
ATCCCTAAAA ATTTGGGGAA TCTTTTTCAC TTGCGGTACA TTAACCTAAG GAATACAAAT 
GTAAAGGCTC TTCCTAAATC TATTGGTGAG TTACATAACT TAGAGACATT GGATTTAAGA 
GAAACACTAG TGCATGAGAT ACCTAGTGAA ATAAACAAGC TGACAAAGTT ACGGCATCTG 
CTTGCTTTTC ATCGCAACTA TGAACAAAAA TATTCTGCTT TGGGCTCTAC AACTGGCGTG 
CTGATTGAAA AAGGTATTAA AAACATGATT TCCCTAAAAA ACCTTTATTA TGTGGAGGTA 
GATCATGGGG GAGTAGATCT CATTGAAGAG ATGAAAATGC TAAGGCAGTT AAGGAAGTTA 
GGTTTAAAGC ATGTAAGAAG GGAACATGGG AATGCCATAA GTGCTGCAGT ACAAGAGATG 
CAACACCTTG AATCACTTAA TATCACTGCA ATAGAAGAGG ATGAAATTAT TGACTTAAAC 
TTTGCGTCAA CCCCACCAAA ACTTCAACGG CTCCATTTGA AAGCTAGACT AGAGAAGTTT 
CCTGATTGGA TTCCAAAATT TGAGTGTCTT GTTCAGATTT GTCTGGCTTT ATCCAAGCTG 
AAAGATGATC CGTTGCAGTC ACTGAAAAAT TTGCCAAATC TTCTGAAGCT CAATTTGTTG 
GAGAATGCCT TTGATGGTGA AATTTTGCAT TTTCAAAATG GAGGATTTCA AATATTGAAA 
GAACTGATTC TCAGTCACTT GAATAGACCA AATTCTATCC TTATAGAGAA AGGAGCTTTG 
CTCTCTCTTG AAAATCTTAA ATTGGAAAGG ATCCCCAAAC TAAAGGATGT GCCTTCTGGC 
ATCAAACACT TGGATAAGCT CAAAGTCATT GACTTAGTTG ATATGCCAGA TGAATTTGTT 
AAGAGCATTG ATCCAGACGG AGGACAAGAC CATTGGATTA TAAACCATGT TCCTATTGTG 
TTCATTCGTC AATGGTTTGG CCCAAAATAC TATGATTATG AGGTTCGCAC TATTAACTCA 
TCCACCAAAG AAGTCCTGAG AGATGAGAAT GCTAAGGTGT CTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024635303.1
CDS	822..3689
Translation	MAETAVSFVLGEVFQFLKEETNLLRSVHRDFSDIKDELESIQVFLKDADRRAADEADTNDGIRTWVKQLREASFRIEDVIDEYLRLMHRANPPRCGSLVGKIARQIKTLIPRHHIASEIQDIKLSIRGIKERSERYNFQISHEQGTSSSISIGERENGKWHDPRLSSLFIEETEVVGIEVPKEELSGWLLDGEAERTVISVVGMGGLGKTTLAKLVFESKKVSAHFDCRACVTVSQSYTVRGLLINMMEQFCRSTKDSLLQMLHKMDDQSLIIEVRQYLQHKRYLIFFDDVWQEDFADQVQFAMPNNNKGSRIIITTRMMYVADFFKKSFLTHIHNLQLLPPNKAWELFCKRVFRYELGGHCPSELEAVSKEIVQKCRQLPLAIVAIGGLLSTKSKTMIEWQKVSENLSLELGRNAHLTCLVKILSLSYDGLPYYLKPCILYFGLYPEDYAINHRRLTRQWIAEGFVKLEERRTPEQVAEEYLSELIQRSLVQVSNVGFEGKVQTCQVHDLMRELIIRKMKDLNFGHCMHEDSESVALGRTRRLSIATNPNNVFRSTNNLQFRAIYVFEEDGSLEHFMGKVCSQSRILKVLDIQGTSLNHIPKNLGNLFHLRYINLRNTNVKALPKSIGELHNLETLDLRETLVHEIPSEINKLTKLRHLLAFHRNYEQKYSALGSTTGVLIEKGIKNMISLKNLYYVEVDHGGVDLIEEMKMLRQLRKLGLKHVRREHGNAISAAVQEMQHLESLNITAIEEDEIIDLNFASTPPKLQRLHLKARLEKFPDWIPKFECLVQICLALSKLKDDPLQSLKNLPNLLKLNLLENAFDGEILHFQNGGFQILKELILSHLNRPNSILIEKGALLSLENLKLERIPKLKDVPSGIKHLDKLKVIDLVDMPDEFVKSIDPDGGQDHWIINHVPIVFIRQWFGPKYYDYEVRTINSSTKEVLRDENAKVSS

Target ORF information:

RefSeq Version	XM_024779535.1
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula disease resistance protein RPM1 (LOC11413331), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024779535.1

ORF Insert Sequence:

ATGGCAGAAA CTGCAGTATC GTTTGTCCTG GGGGAAGTGT TCCAGTTCCT AAAAGAAGAA 
ACAAATCTTC TGAGAAGTGT ACACAGAGAT TTTTCAGACA TCAAAGATGA ACTTGAGAGC 
ATTCAAGTCT TCCTCAAAGA TGCAGACAGA AGGGCTGCGG ATGAAGCAGA CACTAACGAT 
GGAATAAGAA CTTGGGTGAA GCAGCTGAGA GAAGCATCAT TTCGCATTGA AGATGTCATT 
GATGAATACC TTCGGCTAAT GCATAGGGCC AATCCTCCTA GATGCGGATC TTTAGTCGGC 
AAGATTGCAA GGCAAATCAA AACTTTGATT CCCCGTCATC ATATAGCATC TGAAATTCAG 
GACATTAAAT TATCAATTCG TGGAATCAAG GAAAGAAGTG AGAGGTATAA CTTTCAAATT 
TCACACGAAC AAGGAACTTC AAGCAGCATT AGCATAGGAG AGAGAGAAAA TGGAAAATGG 
CATGATCCTC GACTGTCTTC TCTTTTTATT GAAGAAACAG AAGTTGTTGG TATTGAAGTC 
CCAAAAGAAG AACTGTCTGG TTGGTTATTG GATGGTGAAG CTGAGCGCAC GGTGATATCC 
GTGGTAGGAA TGGGAGGCCT AGGAAAAACC ACTCTTGCCA AGCTAGTTTT TGAAAGCAAA 
AAGGTATCAG CACACTTTGA TTGTCGTGCT TGCGTCACAG TTTCTCAATC TTACACTGTG 
AGAGGTTTGT TGATAAACAT GATGGAGCAA TTCTGTCGGA GTACTAAAGA CTCTCTTCTG 
CAGATGTTAC ACAAAATGGA TGACCAGTCA CTAATTATAG AAGTGAGGCA ATACCTGCAA 
CATAAGAGGT ATTTGATATT CTTTGATGAC GTATGGCAAG AAGATTTTGC GGACCAGGTT 
CAATTTGCCA TGCCAAATAA CAATAAAGGT AGTAGGATTA TCATCACCAC TAGAATGATG 
TATGTTGCAG ATTTTTTCAA AAAATCCTTT CTAACTCATA TTCACAACCT GCAACTTTTG 
CCTCCAAACA AAGCATGGGA ACTTTTCTGC AAGAGAGTAT TTAGATATGA GCTTGGTGGA 
CATTGTCCAT CAGAACTTGA GGCTGTGTCA AAAGAAATTG TTCAAAAGTG CAGGCAGCTA 
CCTCTAGCAA TTGTAGCTAT TGGTGGTTTA CTTTCAACAA AATCTAAAAC AATGATTGAA 
TGGCAAAAGG TGAGTGAAAA TCTGAGTCTG GAATTAGGAC GTAATGCCCA TTTAACGTGT 
CTAGTAAAGA TTCTATCACT TAGTTATGAT GGACTTCCTT ATTACTTGAA ACCATGCATT 
TTGTATTTTG GTTTATACCC AGAGGACTAT GCAATTAATC ATAGGAGATT AACTCGACAA 
TGGATAGCTG AAGGGTTTGT AAAATTGGAA GAGAGAAGAA CTCCAGAGCA AGTTGCAGAA 
GAGTACTTGT CTGAATTGAT TCAGAGAAGT TTGGTTCAGG TCTCAAATGT TGGCTTTGAA 
GGAAAAGTTC AAACATGTCA AGTCCATGAT TTAATGCGGG AATTGATCAT TAGAAAAATG 
AAGGATTTAA ATTTCGGTCA TTGCATGCAT GAAGATAGTG AATCAGTTGC ACTTGGAAGA 
ACTAGACGCC TATCTATAGC CACTAATCCC AACAATGTGT TCAGGAGCAC TAATAATTTA 
CAATTTCGGG CTATTTATGT GTTTGAAGAA GATGGATCAT TGGAGCATTT CATGGGTAAA 
GTTTGTTCGC AGTCAAGAAT TTTGAAGGTC CTTGACATTC AAGGGACGTC GTTGAATCAT 
ATCCCTAAAA ATTTGGGGAA TCTTTTTCAC TTGCGGTACA TTAACCTAAG GAATACAAAT 
GTAAAGGCTC TTCCTAAATC TATTGGTGAG TTACATAACT TAGAGACATT GGATTTAAGA 
GAAACACTAG TGCATGAGAT ACCTAGTGAA ATAAACAAGC TGACAAAGTT ACGGCATCTG 
CTTGCTTTTC ATCGCAACTA TGAACAAAAA TATTCTGCTT TGGGCTCTAC AACTGGCGTG 
CTGATTGAAA AAGGTATTAA AAACATGATT TCCCTAAAAA ACCTTTATTA TGTGGAGGTA 
GATCATGGGG GAGTAGATCT CATTGAAGAG ATGAAAATGC TAAGGCAGTT AAGGAAGTTA 
GGTTTAAAGC ATGTAAGAAG GGAACATGGG AATGCCATAA GTGCTGCAGT ACAAGAGATG 
CAACACCTTG AATCACTTAA TATCACTGCA ATAGAAGAGG ATGAAATTAT TGACTTAAAC 
TTTGCGTCAA CCCCACCAAA ACTTCAACGG CTCCATTTGA AAGCTAGACT AGAGAAGTTT 
CCTGATTGGA TTCCAAAATT TGAGTGTCTT GTTCAGATTT GTCTGGCTTT ATCCAAGCTG 
AAAGATGATC CGTTGCAGTC ACTGAAAAAT TTGCCAAATC TTCTGAAGCT CAATTTGTTG 
GAGAATGCCT TTGATGGTGA AATTTTGCAT TTTCAAAATG GAGGATTTCA AATATTGAAA 
GAACTGATTC TCAGTCACTT GAATAGACCA AATTCTATCC TTATAGAGAA AGGAGCTTTG 
CTCTCTCTTG AAAATCTTAA ATTGGAAAGG ATCCCCAAAC TAAAGGATGT GCCTTCTGGC 
ATCAAACACT TGGATAAGCT CAAAGTCATT GACTTAGTTG ATATGCCAGA TGAATTTGTT 
AAGAGCATTG ATCCAGACGG AGGACAAGAC CATTGGATTA TAAACCATGT TCCTATTGTG 
TTCATTCGTC AATGGTTTGG CCCAAAATAC TATGATTATG AGGTTCGCAC TATTAACTCA 
TCCACCAAAG AAGTCCTGAG AGATGAGAAT GCTAAGGTGT CTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC11413331 ( XM_024779536.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_024635304.1 Medicago truncatula disease resistance protein RPM1 (LOC11413331), transcript variant X6, mRNA.

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CloneID	OMe193250
Clone ID Related Accession (Same CDS sequence)	XM_003598360.2 , XM_024779532.1 , XM_024779534.1 , XM_003598360.3 , XM_024779533.1 , XM_024779535.1 , XM_024779536.1
Accession Version	XM_024779536.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	disease resistance protein RPM1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016409.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAA CTGCAGTATC GTTTGTCCTG GGGGAAGTGT TCCAGTTCCT AAAAGAAGAA 
ACAAATCTTC TGAGAAGTGT ACACAGAGAT TTTTCAGACA TCAAAGATGA ACTTGAGAGC 
ATTCAAGTCT TCCTCAAAGA TGCAGACAGA AGGGCTGCGG ATGAAGCAGA CACTAACGAT 
GGAATAAGAA CTTGGGTGAA GCAGCTGAGA GAAGCATCAT TTCGCATTGA AGATGTCATT 
GATGAATACC TTCGGCTAAT GCATAGGGCC AATCCTCCTA GATGCGGATC TTTAGTCGGC 
AAGATTGCAA GGCAAATCAA AACTTTGATT CCCCGTCATC ATATAGCATC TGAAATTCAG 
GACATTAAAT TATCAATTCG TGGAATCAAG GAAAGAAGTG AGAGGTATAA CTTTCAAATT 
TCACACGAAC AAGGAACTTC AAGCAGCATT AGCATAGGAG AGAGAGAAAA TGGAAAATGG 
CATGATCCTC GACTGTCTTC TCTTTTTATT GAAGAAACAG AAGTTGTTGG TATTGAAGTC 
CCAAAAGAAG AACTGTCTGG TTGGTTATTG GATGGTGAAG CTGAGCGCAC GGTGATATCC 
GTGGTAGGAA TGGGAGGCCT AGGAAAAACC ACTCTTGCCA AGCTAGTTTT TGAAAGCAAA 
AAGGTATCAG CACACTTTGA TTGTCGTGCT TGCGTCACAG TTTCTCAATC TTACACTGTG 
AGAGGTTTGT TGATAAACAT GATGGAGCAA TTCTGTCGGA GTACTAAAGA CTCTCTTCTG 
CAGATGTTAC ACAAAATGGA TGACCAGTCA CTAATTATAG AAGTGAGGCA ATACCTGCAA 
CATAAGAGGT ATTTGATATT CTTTGATGAC GTATGGCAAG AAGATTTTGC GGACCAGGTT 
CAATTTGCCA TGCCAAATAA CAATAAAGGT AGTAGGATTA TCATCACCAC TAGAATGATG 
TATGTTGCAG ATTTTTTCAA AAAATCCTTT CTAACTCATA TTCACAACCT GCAACTTTTG 
CCTCCAAACA AAGCATGGGA ACTTTTCTGC AAGAGAGTAT TTAGATATGA GCTTGGTGGA 
CATTGTCCAT CAGAACTTGA GGCTGTGTCA AAAGAAATTG TTCAAAAGTG CAGGCAGCTA 
CCTCTAGCAA TTGTAGCTAT TGGTGGTTTA CTTTCAACAA AATCTAAAAC AATGATTGAA 
TGGCAAAAGG TGAGTGAAAA TCTGAGTCTG GAATTAGGAC GTAATGCCCA TTTAACGTGT 
CTAGTAAAGA TTCTATCACT TAGTTATGAT GGACTTCCTT ATTACTTGAA ACCATGCATT 
TTGTATTTTG GTTTATACCC AGAGGACTAT GCAATTAATC ATAGGAGATT AACTCGACAA 
TGGATAGCTG AAGGGTTTGT AAAATTGGAA GAGAGAAGAA CTCCAGAGCA AGTTGCAGAA 
GAGTACTTGT CTGAATTGAT TCAGAGAAGT TTGGTTCAGG TCTCAAATGT TGGCTTTGAA 
GGAAAAGTTC AAACATGTCA AGTCCATGAT TTAATGCGGG AATTGATCAT TAGAAAAATG 
AAGGATTTAA ATTTCGGTCA TTGCATGCAT GAAGATAGTG AATCAGTTGC ACTTGGAAGA 
ACTAGACGCC TATCTATAGC CACTAATCCC AACAATGTGT TCAGGAGCAC TAATAATTTA 
CAATTTCGGG CTATTTATGT GTTTGAAGAA GATGGATCAT TGGAGCATTT CATGGGTAAA 
GTTTGTTCGC AGTCAAGAAT TTTGAAGGTC CTTGACATTC AAGGGACGTC GTTGAATCAT 
ATCCCTAAAA ATTTGGGGAA TCTTTTTCAC TTGCGGTACA TTAACCTAAG GAATACAAAT 
GTAAAGGCTC TTCCTAAATC TATTGGTGAG TTACATAACT TAGAGACATT GGATTTAAGA 
GAAACACTAG TGCATGAGAT ACCTAGTGAA ATAAACAAGC TGACAAAGTT ACGGCATCTG 
CTTGCTTTTC ATCGCAACTA TGAACAAAAA TATTCTGCTT TGGGCTCTAC AACTGGCGTG 
CTGATTGAAA AAGGTATTAA AAACATGATT TCCCTAAAAA ACCTTTATTA TGTGGAGGTA 
GATCATGGGG GAGTAGATCT CATTGAAGAG ATGAAAATGC TAAGGCAGTT AAGGAAGTTA 
GGTTTAAAGC ATGTAAGAAG GGAACATGGG AATGCCATAA GTGCTGCAGT ACAAGAGATG 
CAACACCTTG AATCACTTAA TATCACTGCA ATAGAAGAGG ATGAAATTAT TGACTTAAAC 
TTTGCGTCAA CCCCACCAAA ACTTCAACGG CTCCATTTGA AAGCTAGACT AGAGAAGTTT 
CCTGATTGGA TTCCAAAATT TGAGTGTCTT GTTCAGATTT GTCTGGCTTT ATCCAAGCTG 
AAAGATGATC CGTTGCAGTC ACTGAAAAAT TTGCCAAATC TTCTGAAGCT CAATTTGTTG 
GAGAATGCCT TTGATGGTGA AATTTTGCAT TTTCAAAATG GAGGATTTCA AATATTGAAA 
GAACTGATTC TCAGTCACTT GAATAGACCA AATTCTATCC TTATAGAGAA AGGAGCTTTG 
CTCTCTCTTG AAAATCTTAA ATTGGAAAGG ATCCCCAAAC TAAAGGATGT GCCTTCTGGC 
ATCAAACACT TGGATAAGCT CAAAGTCATT GACTTAGTTG ATATGCCAGA TGAATTTGTT 
AAGAGCATTG ATCCAGACGG AGGACAAGAC CATTGGATTA TAAACCATGT TCCTATTGTG 
TTCATTCGTC AATGGTTTGG CCCAAAATAC TATGATTATG AGGTTCGCAC TATTAACTCA 
TCCACCAAAG AAGTCCTGAG AGATGAGAAT GCTAAGGTGT CTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024635304.1
CDS	524..3391
Translation	MAETAVSFVLGEVFQFLKEETNLLRSVHRDFSDIKDELESIQVFLKDADRRAADEADTNDGIRTWVKQLREASFRIEDVIDEYLRLMHRANPPRCGSLVGKIARQIKTLIPRHHIASEIQDIKLSIRGIKERSERYNFQISHEQGTSSSISIGERENGKWHDPRLSSLFIEETEVVGIEVPKEELSGWLLDGEAERTVISVVGMGGLGKTTLAKLVFESKKVSAHFDCRACVTVSQSYTVRGLLINMMEQFCRSTKDSLLQMLHKMDDQSLIIEVRQYLQHKRYLIFFDDVWQEDFADQVQFAMPNNNKGSRIIITTRMMYVADFFKKSFLTHIHNLQLLPPNKAWELFCKRVFRYELGGHCPSELEAVSKEIVQKCRQLPLAIVAIGGLLSTKSKTMIEWQKVSENLSLELGRNAHLTCLVKILSLSYDGLPYYLKPCILYFGLYPEDYAINHRRLTRQWIAEGFVKLEERRTPEQVAEEYLSELIQRSLVQVSNVGFEGKVQTCQVHDLMRELIIRKMKDLNFGHCMHEDSESVALGRTRRLSIATNPNNVFRSTNNLQFRAIYVFEEDGSLEHFMGKVCSQSRILKVLDIQGTSLNHIPKNLGNLFHLRYINLRNTNVKALPKSIGELHNLETLDLRETLVHEIPSEINKLTKLRHLLAFHRNYEQKYSALGSTTGVLIEKGIKNMISLKNLYYVEVDHGGVDLIEEMKMLRQLRKLGLKHVRREHGNAISAAVQEMQHLESLNITAIEEDEIIDLNFASTPPKLQRLHLKARLEKFPDWIPKFECLVQICLALSKLKDDPLQSLKNLPNLLKLNLLENAFDGEILHFQNGGFQILKELILSHLNRPNSILIEKGALLSLENLKLERIPKLKDVPSGIKHLDKLKVIDLVDMPDEFVKSIDPDGGQDHWIINHVPIVFIRQWFGPKYYDYEVRTINSSTKEVLRDENAKVSS

Target ORF information:

RefSeq Version	XM_024779536.1
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula disease resistance protein RPM1 (LOC11413331), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024779536.1

ORF Insert Sequence:

ATGGCAGAAA CTGCAGTATC GTTTGTCCTG GGGGAAGTGT TCCAGTTCCT AAAAGAAGAA 
ACAAATCTTC TGAGAAGTGT ACACAGAGAT TTTTCAGACA TCAAAGATGA ACTTGAGAGC 
ATTCAAGTCT TCCTCAAAGA TGCAGACAGA AGGGCTGCGG ATGAAGCAGA CACTAACGAT 
GGAATAAGAA CTTGGGTGAA GCAGCTGAGA GAAGCATCAT TTCGCATTGA AGATGTCATT 
GATGAATACC TTCGGCTAAT GCATAGGGCC AATCCTCCTA GATGCGGATC TTTAGTCGGC 
AAGATTGCAA GGCAAATCAA AACTTTGATT CCCCGTCATC ATATAGCATC TGAAATTCAG 
GACATTAAAT TATCAATTCG TGGAATCAAG GAAAGAAGTG AGAGGTATAA CTTTCAAATT 
TCACACGAAC AAGGAACTTC AAGCAGCATT AGCATAGGAG AGAGAGAAAA TGGAAAATGG 
CATGATCCTC GACTGTCTTC TCTTTTTATT GAAGAAACAG AAGTTGTTGG TATTGAAGTC 
CCAAAAGAAG AACTGTCTGG TTGGTTATTG GATGGTGAAG CTGAGCGCAC GGTGATATCC 
GTGGTAGGAA TGGGAGGCCT AGGAAAAACC ACTCTTGCCA AGCTAGTTTT TGAAAGCAAA 
AAGGTATCAG CACACTTTGA TTGTCGTGCT TGCGTCACAG TTTCTCAATC TTACACTGTG 
AGAGGTTTGT TGATAAACAT GATGGAGCAA TTCTGTCGGA GTACTAAAGA CTCTCTTCTG 
CAGATGTTAC ACAAAATGGA TGACCAGTCA CTAATTATAG AAGTGAGGCA ATACCTGCAA 
CATAAGAGGT ATTTGATATT CTTTGATGAC GTATGGCAAG AAGATTTTGC GGACCAGGTT 
CAATTTGCCA TGCCAAATAA CAATAAAGGT AGTAGGATTA TCATCACCAC TAGAATGATG 
TATGTTGCAG ATTTTTTCAA AAAATCCTTT CTAACTCATA TTCACAACCT GCAACTTTTG 
CCTCCAAACA AAGCATGGGA ACTTTTCTGC AAGAGAGTAT TTAGATATGA GCTTGGTGGA 
CATTGTCCAT CAGAACTTGA GGCTGTGTCA AAAGAAATTG TTCAAAAGTG CAGGCAGCTA 
CCTCTAGCAA TTGTAGCTAT TGGTGGTTTA CTTTCAACAA AATCTAAAAC AATGATTGAA 
TGGCAAAAGG TGAGTGAAAA TCTGAGTCTG GAATTAGGAC GTAATGCCCA TTTAACGTGT 
CTAGTAAAGA TTCTATCACT TAGTTATGAT GGACTTCCTT ATTACTTGAA ACCATGCATT 
TTGTATTTTG GTTTATACCC AGAGGACTAT GCAATTAATC ATAGGAGATT AACTCGACAA 
TGGATAGCTG AAGGGTTTGT AAAATTGGAA GAGAGAAGAA CTCCAGAGCA AGTTGCAGAA 
GAGTACTTGT CTGAATTGAT TCAGAGAAGT TTGGTTCAGG TCTCAAATGT TGGCTTTGAA 
GGAAAAGTTC AAACATGTCA AGTCCATGAT TTAATGCGGG AATTGATCAT TAGAAAAATG 
AAGGATTTAA ATTTCGGTCA TTGCATGCAT GAAGATAGTG AATCAGTTGC ACTTGGAAGA 
ACTAGACGCC TATCTATAGC CACTAATCCC AACAATGTGT TCAGGAGCAC TAATAATTTA 
CAATTTCGGG CTATTTATGT GTTTGAAGAA GATGGATCAT TGGAGCATTT CATGGGTAAA 
GTTTGTTCGC AGTCAAGAAT TTTGAAGGTC CTTGACATTC AAGGGACGTC GTTGAATCAT 
ATCCCTAAAA ATTTGGGGAA TCTTTTTCAC TTGCGGTACA TTAACCTAAG GAATACAAAT 
GTAAAGGCTC TTCCTAAATC TATTGGTGAG TTACATAACT TAGAGACATT GGATTTAAGA 
GAAACACTAG TGCATGAGAT ACCTAGTGAA ATAAACAAGC TGACAAAGTT ACGGCATCTG 
CTTGCTTTTC ATCGCAACTA TGAACAAAAA TATTCTGCTT TGGGCTCTAC AACTGGCGTG 
CTGATTGAAA AAGGTATTAA AAACATGATT TCCCTAAAAA ACCTTTATTA TGTGGAGGTA 
GATCATGGGG GAGTAGATCT CATTGAAGAG ATGAAAATGC TAAGGCAGTT AAGGAAGTTA 
GGTTTAAAGC ATGTAAGAAG GGAACATGGG AATGCCATAA GTGCTGCAGT ACAAGAGATG 
CAACACCTTG AATCACTTAA TATCACTGCA ATAGAAGAGG ATGAAATTAT TGACTTAAAC 
TTTGCGTCAA CCCCACCAAA ACTTCAACGG CTCCATTTGA AAGCTAGACT AGAGAAGTTT 
CCTGATTGGA TTCCAAAATT TGAGTGTCTT GTTCAGATTT GTCTGGCTTT ATCCAAGCTG 
AAAGATGATC CGTTGCAGTC ACTGAAAAAT TTGCCAAATC TTCTGAAGCT CAATTTGTTG 
GAGAATGCCT TTGATGGTGA AATTTTGCAT TTTCAAAATG GAGGATTTCA AATATTGAAA 
GAACTGATTC TCAGTCACTT GAATAGACCA AATTCTATCC TTATAGAGAA AGGAGCTTTG 
CTCTCTCTTG AAAATCTTAA ATTGGAAAGG ATCCCCAAAC TAAAGGATGT GCCTTCTGGC 
ATCAAACACT TGGATAAGCT CAAAGTCATT GACTTAGTTG ATATGCCAGA TGAATTTGTT 
AAGAGCATTG ATCCAGACGG AGGACAAGAC CATTGGATTA TAAACCATGT TCCTATTGTG 
TTCATTCGTC AATGGTTTGG CCCAAAATAC TATGATTATG AGGTTCGCAC TATTAACTCA 
TCCACCAAAG AAGTCCTGAG AGATGAGAAT GCTAAGGTGT CTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
BMC genomics15312(2014 Apr)
Tang H,Krishnakumar V,Bidwell S,Rosen B,Chan A,Zhou S,Gentzbittel L,Childs KL,Yandell M,Gundlach H,Mayer KF,Schwartz DC,Town CD

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Nature480(7378)520-4(2011 Nov)
Young ND,Debell? F,Oldroyd GE,Geurts R,Cannon SB,Udvardi MK,Benedito VA,Mayer KF,Gouzy J,Schoof H,Van de Peer Y,Proost S,Cook DR,Meyers BC,Spannagl M,Cheung F,De Mita S,Krishnakumar V,Gundlach H,Zhou S,Mudge J,Bharti AK,Murray JD,Naoumkina MA,Rosen B,Silverstein KA,Tang H,Rombauts S,Zhao PX,Zhou P,Barbe V,Bardou P,Bechner M,Bellec A,Berger A,Berg?s H,Bidwell S,Bisseling T,Choisne N,Couloux A,Denny R,Deshpande S,Dai X,Doyle JJ,Dudez AM,Farmer AD,Fouteau S,Franken C,Gibelin C,Gish J,Goldstein S,Gonz?lez AJ,Green PJ,Hallab A,Hartog M,Hua A,Humphray SJ,Jeong DH,Jing Y,J?cker A,Kenton SM,Kim DJ,Klee K,Lai H,Lang C,Lin S,Macmil SL,Magdelenat G,Matthews L,McCorrison J,Monaghan EL,Mun JH,Najar FZ,Nicholson C,Noirot C,O'Bleness M,Paule CR,Poulain J,Prion F,Qin B,Qu C,Retzel EF,Riddle C,Sallet E,Samain S,Samson N,Sanders I,Saurat O,Scarpelli C,Schiex T,Segurens B,Severin AJ,Sherrier DJ,Shi R,Sims S,Singer SR,Sinharoy S,Sterck L,Viollet A,Wang BB,Wang K,Wang M,Wang X,Warfsmann J,Weissenbach J,White DD,White JD,Wiley GB,Wincker P,Xing Y,Yang L,Yao Z,Ying F,Zhai J,Zhou L,Zuber A,D?nari? J,Dixon RA,May GD,Schwartz DC,Rogers J,Qu?tier F,Town CD,Roe BA

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LOC11413331 cDNA ORF clone, Medicago truncatula(barrel medic)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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