MTR_8g085210 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene
Clones
gRNAs

Summary

Gene Symbol	MTR_8g085210
Entrez Gene ID	11411364
Full Name	beta-galactosidase
Gene Type	protein-coding
Organism	Medicago truncatula(barrel medic)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003629615.3	XP_003629663.2	beta-galactosidase 9
XM_003629615.2	XP_003629663.2	beta-galactosidase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
Tang H, Krishnakumar V, Bidwell S, Rosen B, Chan A, Zhou S, Gentzbittel L, Childs KL, Yandell M, Gundlach H, Mayer KF, Schwartz DC, Town CD
BMC genomics15312(2014 Apr)

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Young ND, Debell? F, Oldroyd GE, Geurts R, Cannon SB, Udvardi MK, Benedito VA, Mayer KF, Gouzy J, Schoof H, Van de Peer Y, Proost S, Cook DR, Meyers BC, Spannagl M, Cheung F, De Mita S, Krishnakumar V, Gundlach H, Zhou S, Mudge J, Bharti AK, Murray JD, Naoumkina MA, Rosen B, Silverstein KA, Tang H, Rombauts S, Zhao PX, Zhou P, Barbe V, Bardou P, Bechner M, Bellec A, Berger A, Berg?s H, Bidwell S, Bisseling T, Choisne N, Couloux A, Denny R, Deshpande S, Dai X, Doyle JJ, Dudez AM, Farmer AD, Fouteau S, Franken C, Gibelin C, Gish J, Goldstein S, Gonz?lez AJ, Green PJ, Hallab A, Hartog M, Hua A, Humphray SJ, Jeong DH, Jing Y, J?cker A, Kenton SM, Kim DJ, Klee K, Lai H, Lang C, Lin S, Macmil SL, Magdelenat G, Matthews L, McCorrison J, Monaghan EL, Mun JH, Najar FZ, Nicholson C, Noirot C, O'Bleness M, Paule CR, Poulain J, Prion F, Qin B, Qu C, Retzel EF, Riddle C, Sallet E, Samain S, Samson N, Sanders I, Saurat O, Scarpelli C, Schiex T, Segurens B, Severin AJ, Sherrier DJ, Shi R, Sims S, Singer SR, Sinharoy S, Sterck L, Viollet A, Wang BB, Wang K, Wang M, Wang X, Warfsmann J, Weissenbach J, White DD, White JD, Wiley GB, Wincker P, Xing Y, Yang L, Yao Z, Ying F, Zhai J, Zhou L, Zuber A, D?nari? J, Dixon RA, May GD, Schwartz DC, Rogers J, Qu?tier F, Town CD, Roe BA
Nature480(7378)520-4(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MTR_8g085210 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MTR_8g085210 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe161320	XM_003629615.3 Latest version!	Medicago truncatula beta-galactosidase 9 (LOC11411364), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OMe161320	XM_003629615.2	Medicago truncatula beta-galactosidase mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MTR_8g085210 ( XM_003629615.3 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_003629663.2 Medicago truncatula beta-galactosidase 9 (LOC11411364), mRNA.

Price & Availability↑

CloneID	OMe161320
Clone ID Related Accession (Same CDS sequence)	XM_003629615.3 , XM_003629615.2
Accession Version	XM_003629615.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	beta-galactosidase 9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016414.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2018 this sequence version replaced XM_003629615.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATAT CGTTGCAGAA TTATTCCAAT TCCAAAGCAG GAACAATAAG AGGAAGAACA 
GTGGTATTCA CATGGTTTTG TGTGTGTGTT TTCGTAGCTT CAATTATAGT AGCAGGAGCA 
GAAGCTGCGT GGTTCAAACC GTTCAACGTC ACCTACGATC ATCGTGCTCT CATCATCGAC 
GGTCACCGTC GCATGCTCAT CTCCGCCGGT ATTCACTATC CCCGCGCCAC TCCCGAGATG 
TGGCCTGATC TCATAGCAAA GGCCAAGGAA GGTGGTGTAG ATGTAATCGA AACTTATGTG 
TTTTGGAATG GGCACCAGCC TGTTAAAGGA CAGTATAATT TTGAAGGGAG ATACGATCTT 
GTGAAGTTTG CAAAGCTAGT TGCATCCAAT GGACTCTATT TCTTTCTCCG TATAGGCCCT 
TATGCATGTG CTGAGTGGAA CTTCGGTGGT TTCCCGGTGT GGTTGCGTGA TATCCCGGGT 
ATTGAATTTC GAACCAATAA TGCACCTTTC AAGGAGGAAA TGAAGCGGTT TGTTTCCAAG 
GTGGTGAATC TTATGAGAGA GGAAATGCTG TTTTCTTGGC AAGGTGGACC GATAATTTTG 
TTACAGATTG AGAATGAATA TGGGAACCTT GAGAGCTCCT ATGGCAATGA AGGCAAAGAG 
TACGTTAAAT GGGCGGCTAG CATGGCTCTA AGTCTTGGTG CTGGTGTTCC ATGGGTCATG 
TGCAAGCAAC CTGATGCTCC ATATGATATT ATTGATACAT GCAATGCATA CTACTGTGAT 
GGATTTAAAC CAAACTCTCG TAATAAACCA ATTTTTTGGA CAGAGAACTG GGATGGATGG 
TACACACAGT GGGGTGAAAG ATTGCCTCAT AGACCTGTTG AAGACCTTGC ATTTGCTGTT 
GCGCGTTTTT TCCAGCGTGG AGGAAGTCTT CAAAATTATT ATATGTATTT TGGTGGAACA 
AATTTTGGCC GCACTGCAGG GGGACCCTTG CAAATTACAA GTTATGATTA TGATGCTCCA 
ATTGATGAGT ATGGTCTGCT GAATGAGCCA AAATGGGGTC ACTTGAAAGA TTTGCATGCG 
GCTTTAAAGC TTTGTGAGCC TGCTCTAGTG GCCGCTGACT CACCAACATA TATAAAACTG 
GGTTCAAAGC AGGAGGCACA TGTATACCAG GAAAATGTCC ACCGTGAAGG ATTGAACTTG 
TCTATATCTC AAATCTCTAA CAAATGCTCA GCGTTCCTTG CCAACATTGA TGAACGTAAA 
GCAGCAACTG TGACCTTTCG TGGTCAGACA TACACTTTAC CACCATGGTC TGTGAGCATA 
TTGCCAGATT GCAGGAGTGC GATTTTCAAT ACTGCTAAGG TTGGAGCACA AACTTCAGTC 
AAACTGGTAG GGTCCAATTT GCCACTCACA TCTAATCTTT TGCTATCCCA GCAATCGATA 
GATCACAATG GCATCTCCCA TATCTCAAAG TCTTGGATGA CTACAAAAGA GCCGATCAAT 
ATATGGATTA ACAGCAGTTT CACTGCAGAA GGTATATGGG AGCACTTAAA TGTAACAAAA 
GATCAATCTG ACTATCTTTG GTATTCAACC AGAATATATG TTTCAGATGG TGATATCTTG 
TTTTGGAAAG AAAATGCTGC TCATCCAAAA CTTGCTATAG ACAGTGTCCG TGATATACTG 
CGTGTATTTG TGAATGGCCA ACTTATAGGT AATGTTGTTG GCCATTGGGT CAAGGCGGTC 
CAGACTCTTC AATTTCAGCC AGGCTACAAT GATTTAACAC TGTTAACTCA AACAGTAGGC 
TTGCAGAATT ACGGTGCCTT CATTGAGAAA GATGGAGCTG GAATAAGAGG TACAATCAAG 
ATCACTGGAT TTGAAAATGG GCACATAGAT CTTTCGAAAC CATTATGGAC CTACCAGGTT 
GGGCTTCAAG GTGAGTTTTT AAAATTTTAC AATGAAGAGA GTGAAAATGC AGGATGGGTA 
GAATTGACTC CTGATGCCAT TCCATCCACG TTTACATGGT ACAAGACATA CTTTGATGTC 
CCTGGTGGAA ATGATCCAGT TGCTCTTGAC TTGGAAAGCA TGGGGAAAGG TCAAGCTTGG 
GTGAATGGTC ACCATATCGG AAGATATTGG ACCCGGGTTT CCCCAAAAAC TGGATGCCAA 
GTCTGTGATT ATCGCGGGGC TTACGATTCT GACAAGTGCA CAACCAATTG TGGAAAACCA 
ACTCAAACTT TGTACCATGT GCCACGATCA TGGTTGAAAG CATCCAACAA TTTCCTTGTT 
ATCTTGGAGG AAACTGGAGG AAATCCCTTA GGAATTTCTG TCAAGTTACA TTCAGCCAGT 
ATAGTTTGTG CTCAAGTGTC ACAATCCTAC TATCCACCTA TGCAGAAGTT GTTGAATGCA 
AGTCTCCTTG GTCAACAAGA AGTTTCTTCA AATGATATGA TACCTGAAAT GAACTTGCGC 
TGTCGAGATG GAAATATAAT TTCCTCCATA ACATTTGCTA GCTTTGGAAC TCCTGGAGGT 
AGTTGTCAGA GTTTTTCTAG AGGGAACTGT CATGCTCCTA GTTCAAAATC TATAGTTTCC 
AAGGCTTGCC TGGGGAAAAG GAGCTGCTCC ATTAAAATTT CAAGTGATGT ATTCGGAGGT 
GACCCCTGCC AGGATGTTGT GAAAACATTG TCTGTGGAAG CAAGGTGCAT CACCATCACT 
AATGGTTCCT TCCAACTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003629663.2
CDS	220..2940
Translation	MKISLQNYSNSKAGTIRGRTVVFTWFCVCVFVASIIVAGAEAAWFKPFNVTYDHRALIIDGHRRMLISAGIHYPRATPEMWPDLIAKAKEGGVDVIETYVFWNGHQPVKGQYNFEGRYDLVKFAKLVASNGLYFFLRIGPYACAEWNFGGFPVWLRDIPGIEFRTNNAPFKEEMKRFVSKVVNLMREEMLFSWQGGPIILLQIENEYGNLESSYGNEGKEYVKWAASMALSLGAGVPWVMCKQPDAPYDIIDTCNAYYCDGFKPNSRNKPIFWTENWDGWYTQWGERLPHRPVEDLAFAVARFFQRGGSLQNYYMYFGGTNFGRTAGGPLQITSYDYDAPIDEYGLLNEPKWGHLKDLHAALKLCEPALVAADSPTYIKLGSKQEAHVYQENVHREGLNLSISQISNKCSAFLANIDERKAATVTFRGQTYTLPPWSVSILPDCRSAIFNTAKVGAQTSVKLVGSNLPLTSNLLLSQQSIDHNGISHISKSWMTTKEPINIWINSSFTAEGIWEHLNVTKDQSDYLWYSTRIYVSDGDILFWKENAAHPKLAIDSVRDILRVFVNGQLIGNVVGHWVKAVQTLQFQPGYNDLTLLTQTVGLQNYGAFIEKDGAGIRGTIKITGFENGHIDLSKPLWTYQVGLQGEFLKFYNEESENAGWVELTPDAIPSTFTWYKTYFDVPGGNDPVALDLESMGKGQAWVNGHHIGRYWTRVSPKTGCQVCDYRGAYDSDKCTTNCGKPTQTLYHVPRSWLKASNNFLVILEETGGNPLGISVKLHSASIVCAQVSQSYYPPMQKLLNASLLGQQEVSSNDMIPEMNLRCRDGNIISSITFASFGTPGGSCQSFSRGNCHAPSSKSIVSKACLGKRSCSIKISSDVFGGDPCQDVVKTLSVEARCITITNGSFQL

Target ORF information:

RefSeq Version	XM_003629615.3
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula beta-galactosidase 9 (LOC11411364), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003629615.3

ORF Insert Sequence:

ATGAAGATAT CGTTGCAGAA TTATTCCAAT TCCAAAGCAG GAACAATAAG AGGAAGAACA 
GTGGTATTCA CATGGTTTTG TGTGTGTGTT TTCGTAGCTT CAATTATAGT AGCAGGAGCA 
GAAGCTGCGT GGTTCAAACC GTTCAACGTC ACCTACGATC ATCGTGCTCT CATCATCGAC 
GGTCACCGTC GCATGCTCAT CTCCGCCGGT ATTCACTATC CCCGCGCCAC TCCCGAGATG 
TGGCCTGATC TCATAGCAAA GGCCAAGGAA GGTGGTGTAG ATGTAATCGA AACTTATGTG 
TTTTGGAATG GGCACCAGCC TGTTAAAGGA CAGTATAATT TTGAAGGGAG ATACGATCTT 
GTGAAGTTTG CAAAGCTAGT TGCATCCAAT GGACTCTATT TCTTTCTCCG TATAGGCCCT 
TATGCATGTG CTGAGTGGAA CTTCGGTGGT TTCCCGGTGT GGTTGCGTGA TATCCCGGGT 
ATTGAATTTC GAACCAATAA TGCACCTTTC AAGGAGGAAA TGAAGCGGTT TGTTTCCAAG 
GTGGTGAATC TTATGAGAGA GGAAATGCTG TTTTCTTGGC AAGGTGGACC GATAATTTTG 
TTACAGATTG AGAATGAATA TGGGAACCTT GAGAGCTCCT ATGGCAATGA AGGCAAAGAG 
TACGTTAAAT GGGCGGCTAG CATGGCTCTA AGTCTTGGTG CTGGTGTTCC ATGGGTCATG 
TGCAAGCAAC CTGATGCTCC ATATGATATT ATTGATACAT GCAATGCATA CTACTGTGAT 
GGATTTAAAC CAAACTCTCG TAATAAACCA ATTTTTTGGA CAGAGAACTG GGATGGATGG 
TACACACAGT GGGGTGAAAG ATTGCCTCAT AGACCTGTTG AAGACCTTGC ATTTGCTGTT 
GCGCGTTTTT TCCAGCGTGG AGGAAGTCTT CAAAATTATT ATATGTATTT TGGTGGAACA 
AATTTTGGCC GCACTGCAGG GGGACCCTTG CAAATTACAA GTTATGATTA TGATGCTCCA 
ATTGATGAGT ATGGTCTGCT GAATGAGCCA AAATGGGGTC ACTTGAAAGA TTTGCATGCG 
GCTTTAAAGC TTTGTGAGCC TGCTCTAGTG GCCGCTGACT CACCAACATA TATAAAACTG 
GGTTCAAAGC AGGAGGCACA TGTATACCAG GAAAATGTCC ACCGTGAAGG ATTGAACTTG 
TCTATATCTC AAATCTCTAA CAAATGCTCA GCGTTCCTTG CCAACATTGA TGAACGTAAA 
GCAGCAACTG TGACCTTTCG TGGTCAGACA TACACTTTAC CACCATGGTC TGTGAGCATA 
TTGCCAGATT GCAGGAGTGC GATTTTCAAT ACTGCTAAGG TTGGAGCACA AACTTCAGTC 
AAACTGGTAG GGTCCAATTT GCCACTCACA TCTAATCTTT TGCTATCCCA GCAATCGATA 
GATCACAATG GCATCTCCCA TATCTCAAAG TCTTGGATGA CTACAAAAGA GCCGATCAAT 
ATATGGATTA ACAGCAGTTT CACTGCAGAA GGTATATGGG AGCACTTAAA TGTAACAAAA 
GATCAATCTG ACTATCTTTG GTATTCAACC AGAATATATG TTTCAGATGG TGATATCTTG 
TTTTGGAAAG AAAATGCTGC TCATCCAAAA CTTGCTATAG ACAGTGTCCG TGATATACTG 
CGTGTATTTG TGAATGGCCA ACTTATAGGT AATGTTGTTG GCCATTGGGT CAAGGCGGTC 
CAGACTCTTC AATTTCAGCC AGGCTACAAT GATTTAACAC TGTTAACTCA AACAGTAGGC 
TTGCAGAATT ACGGTGCCTT CATTGAGAAA GATGGAGCTG GAATAAGAGG TACAATCAAG 
ATCACTGGAT TTGAAAATGG GCACATAGAT CTTTCGAAAC CATTATGGAC CTACCAGGTT 
GGGCTTCAAG GTGAGTTTTT AAAATTTTAC AATGAAGAGA GTGAAAATGC AGGATGGGTA 
GAATTGACTC CTGATGCCAT TCCATCCACG TTTACATGGT ACAAGACATA CTTTGATGTC 
CCTGGTGGAA ATGATCCAGT TGCTCTTGAC TTGGAAAGCA TGGGGAAAGG TCAAGCTTGG 
GTGAATGGTC ACCATATCGG AAGATATTGG ACCCGGGTTT CCCCAAAAAC TGGATGCCAA 
GTCTGTGATT ATCGCGGGGC TTACGATTCT GACAAGTGCA CAACCAATTG TGGAAAACCA 
ACTCAAACTT TGTACCATGT GCCACGATCA TGGTTGAAAG CATCCAACAA TTTCCTTGTT 
ATCTTGGAGG AAACTGGAGG AAATCCCTTA GGAATTTCTG TCAAGTTACA TTCAGCCAGT 
ATAGTTTGTG CTCAAGTGTC ACAATCCTAC TATCCACCTA TGCAGAAGTT GTTGAATGCA 
AGTCTCCTTG GTCAACAAGA AGTTTCTTCA AATGATATGA TACCTGAAAT GAACTTGCGC 
TGTCGAGATG GAAATATAAT TTCCTCCATA ACATTTGCTA GCTTTGGAAC TCCTGGAGGT 
AGTTGTCAGA GTTTTTCTAG AGGGAACTGT CATGCTCCTA GTTCAAAATC TATAGTTTCC 
AAGGCTTGCC TGGGGAAAAG GAGCTGCTCC ATTAAAATTT CAAGTGATGT ATTCGGAGGT 
GACCCCTGCC AGGATGTTGT GAAAACATTG TCTGTGGAAG CAAGGTGCAT CACCATCACT 
AATGGTTCCT TCCAACTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTR_8g085210 ( XM_003629615.2 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_003629663.2 Medicago truncatula beta-galactosidase mRNA.

Price & Availability↑

CloneID	OMe161320
Clone ID Related Accession (Same CDS sequence)	XM_003629615.3 , XM_003629615.2
Accession Version	XM_003629615.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-24
Organism	Medicago truncatula(barrel medic)
Product	beta-galactosidase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_016414). On Aug 25, 2015 this sequence version replaced XM_003629615.1. ##Genome-Annotation-Data-START## Annotation Provider :: JCVI Annotation Version :: Mt4.0v2 Annotation Pipeline :: JCVI Eukaryotic Genome Annotation Pipeline Annotation Method :: EVidenceModeler (EVM), PASA Features Annotated :: Gene; mRNA; CDS; tRNA URL :: http://www.jcvi.org/medicago ##Genome-Annotation-Data-END##

ATGAAGATAT CGTTGCAGAA TTATTCCAAT TCCAAAGCAG GAACAATAAG AGGAAGAACA 
GTGGTATTCA CATGGTTTTG TGTGTGTGTT TTCGTAGCTT CAATTATAGT AGCAGGAGCA 
GAAGCTGCGT GGTTCAAACC GTTCAACGTC ACCTACGATC ATCGTGCTCT CATCATCGAC 
GGTCACCGTC GCATGCTCAT CTCCGCCGGT ATTCACTATC CCCGCGCCAC TCCCGAGATG 
TGGCCTGATC TCATAGCAAA GGCCAAGGAA GGTGGTGTAG ATGTAATCGA AACTTATGTG 
TTTTGGAATG GGCACCAGCC TGTTAAAGGA CAGTATAATT TTGAAGGGAG ATACGATCTT 
GTGAAGTTTG CAAAGCTAGT TGCATCCAAT GGACTCTATT TCTTTCTCCG TATAGGCCCT 
TATGCATGTG CTGAGTGGAA CTTCGGTGGT TTCCCGGTGT GGTTGCGTGA TATCCCGGGT 
ATTGAATTTC GAACCAATAA TGCACCTTTC AAGGAGGAAA TGAAGCGGTT TGTTTCCAAG 
GTGGTGAATC TTATGAGAGA GGAAATGCTG TTTTCTTGGC AAGGTGGACC GATAATTTTG 
TTACAGATTG AGAATGAATA TGGGAACCTT GAGAGCTCCT ATGGCAATGA AGGCAAAGAG 
TACGTTAAAT GGGCGGCTAG CATGGCTCTA AGTCTTGGTG CTGGTGTTCC ATGGGTCATG 
TGCAAGCAAC CTGATGCTCC ATATGATATT ATTGATACAT GCAATGCATA CTACTGTGAT 
GGATTTAAAC CAAACTCTCG TAATAAACCA ATTTTTTGGA CAGAGAACTG GGATGGATGG 
TACACACAGT GGGGTGAAAG ATTGCCTCAT AGACCTGTTG AAGACCTTGC ATTTGCTGTT 
GCGCGTTTTT TCCAGCGTGG AGGAAGTCTT CAAAATTATT ATATGTATTT TGGTGGAACA 
AATTTTGGCC GCACTGCAGG GGGACCCTTG CAAATTACAA GTTATGATTA TGATGCTCCA 
ATTGATGAGT ATGGTCTGCT GAATGAGCCA AAATGGGGTC ACTTGAAAGA TTTGCATGCG 
GCTTTAAAGC TTTGTGAGCC TGCTCTAGTG GCCGCTGACT CACCAACATA TATAAAACTG 
GGTTCAAAGC AGGAGGCACA TGTATACCAG GAAAATGTCC ACCGTGAAGG ATTGAACTTG 
TCTATATCTC AAATCTCTAA CAAATGCTCA GCGTTCCTTG CCAACATTGA TGAACGTAAA 
GCAGCAACTG TGACCTTTCG TGGTCAGACA TACACTTTAC CACCATGGTC TGTGAGCATA 
TTGCCAGATT GCAGGAGTGC GATTTTCAAT ACTGCTAAGG TTGGAGCACA AACTTCAGTC 
AAACTGGTAG GGTCCAATTT GCCACTCACA TCTAATCTTT TGCTATCCCA GCAATCGATA 
GATCACAATG GCATCTCCCA TATCTCAAAG TCTTGGATGA CTACAAAAGA GCCGATCAAT 
ATATGGATTA ACAGCAGTTT CACTGCAGAA GGTATATGGG AGCACTTAAA TGTAACAAAA 
GATCAATCTG ACTATCTTTG GTATTCAACC AGAATATATG TTTCAGATGG TGATATCTTG 
TTTTGGAAAG AAAATGCTGC TCATCCAAAA CTTGCTATAG ACAGTGTCCG TGATATACTG 
CGTGTATTTG TGAATGGCCA ACTTATAGGT AATGTTGTTG GCCATTGGGT CAAGGCGGTC 
CAGACTCTTC AATTTCAGCC AGGCTACAAT GATTTAACAC TGTTAACTCA AACAGTAGGC 
TTGCAGAATT ACGGTGCCTT CATTGAGAAA GATGGAGCTG GAATAAGAGG TACAATCAAG 
ATCACTGGAT TTGAAAATGG GCACATAGAT CTTTCGAAAC CATTATGGAC CTACCAGGTT 
GGGCTTCAAG GTGAGTTTTT AAAATTTTAC AATGAAGAGA GTGAAAATGC AGGATGGGTA 
GAATTGACTC CTGATGCCAT TCCATCCACG TTTACATGGT ACAAGACATA CTTTGATGTC 
CCTGGTGGAA ATGATCCAGT TGCTCTTGAC TTGGAAAGCA TGGGGAAAGG TCAAGCTTGG 
GTGAATGGTC ACCATATCGG AAGATATTGG ACCCGGGTTT CCCCAAAAAC TGGATGCCAA 
GTCTGTGATT ATCGCGGGGC TTACGATTCT GACAAGTGCA CAACCAATTG TGGAAAACCA 
ACTCAAACTT TGTACCATGT GCCACGATCA TGGTTGAAAG CATCCAACAA TTTCCTTGTT 
ATCTTGGAGG AAACTGGAGG AAATCCCTTA GGAATTTCTG TCAAGTTACA TTCAGCCAGT 
ATAGTTTGTG CTCAAGTGTC ACAATCCTAC TATCCACCTA TGCAGAAGTT GTTGAATGCA 
AGTCTCCTTG GTCAACAAGA AGTTTCTTCA AATGATATGA TACCTGAAAT GAACTTGCGC 
TGTCGAGATG GAAATATAAT TTCCTCCATA ACATTTGCTA GCTTTGGAAC TCCTGGAGGT 
AGTTGTCAGA GTTTTTCTAG AGGGAACTGT CATGCTCCTA GTTCAAAATC TATAGTTTCC 
AAGGCTTGCC TGGGGAAAAG GAGCTGCTCC ATTAAAATTT CAAGTGATGT ATTCGGAGGT 
GACCCCTGCC AGGATGTTGT GAAAACATTG TCTGTGGAAG CAAGGTGCAT CACCATCACT 
AATGGTTCCT TCCAACTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003629663.2
CDS	257..2977
Translation	MKISLQNYSNSKAGTIRGRTVVFTWFCVCVFVASIIVAGAEAAWFKPFNVTYDHRALIIDGHRRMLISAGIHYPRATPEMWPDLIAKAKEGGVDVIETYVFWNGHQPVKGQYNFEGRYDLVKFAKLVASNGLYFFLRIGPYACAEWNFGGFPVWLRDIPGIEFRTNNAPFKEEMKRFVSKVVNLMREEMLFSWQGGPIILLQIENEYGNLESSYGNEGKEYVKWAASMALSLGAGVPWVMCKQPDAPYDIIDTCNAYYCDGFKPNSRNKPIFWTENWDGWYTQWGERLPHRPVEDLAFAVARFFQRGGSLQNYYMYFGGTNFGRTAGGPLQITSYDYDAPIDEYGLLNEPKWGHLKDLHAALKLCEPALVAADSPTYIKLGSKQEAHVYQENVHREGLNLSISQISNKCSAFLANIDERKAATVTFRGQTYTLPPWSVSILPDCRSAIFNTAKVGAQTSVKLVGSNLPLTSNLLLSQQSIDHNGISHISKSWMTTKEPINIWINSSFTAEGIWEHLNVTKDQSDYLWYSTRIYVSDGDILFWKENAAHPKLAIDSVRDILRVFVNGQLIGNVVGHWVKAVQTLQFQPGYNDLTLLTQTVGLQNYGAFIEKDGAGIRGTIKITGFENGHIDLSKPLWTYQVGLQGEFLKFYNEESENAGWVELTPDAIPSTFTWYKTYFDVPGGNDPVALDLESMGKGQAWVNGHHIGRYWTRVSPKTGCQVCDYRGAYDSDKCTTNCGKPTQTLYHVPRSWLKASNNFLVILEETGGNPLGISVKLHSASIVCAQVSQSYYPPMQKLLNASLLGQQEVSSNDMIPEMNLRCRDGNIISSITFASFGTPGGSCQSFSRGNCHAPSSKSIVSKACLGKRSCSIKISSDVFGGDPCQDVVKTLSVEARCITITNGSFQL

Target ORF information:

RefSeq Version	XM_003629615.2
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula beta-galactosidase mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003629615.2

ORF Insert Sequence:

ATGAAGATAT CGTTGCAGAA TTATTCCAAT TCCAAAGCAG GAACAATAAG AGGAAGAACA 
GTGGTATTCA CATGGTTTTG TGTGTGTGTT TTCGTAGCTT CAATTATAGT AGCAGGAGCA 
GAAGCTGCGT GGTTCAAACC GTTCAACGTC ACCTACGATC ATCGTGCTCT CATCATCGAC 
GGTCACCGTC GCATGCTCAT CTCCGCCGGT ATTCACTATC CCCGCGCCAC TCCCGAGATG 
TGGCCTGATC TCATAGCAAA GGCCAAGGAA GGTGGTGTAG ATGTAATCGA AACTTATGTG 
TTTTGGAATG GGCACCAGCC TGTTAAAGGA CAGTATAATT TTGAAGGGAG ATACGATCTT 
GTGAAGTTTG CAAAGCTAGT TGCATCCAAT GGACTCTATT TCTTTCTCCG TATAGGCCCT 
TATGCATGTG CTGAGTGGAA CTTCGGTGGT TTCCCGGTGT GGTTGCGTGA TATCCCGGGT 
ATTGAATTTC GAACCAATAA TGCACCTTTC AAGGAGGAAA TGAAGCGGTT TGTTTCCAAG 
GTGGTGAATC TTATGAGAGA GGAAATGCTG TTTTCTTGGC AAGGTGGACC GATAATTTTG 
TTACAGATTG AGAATGAATA TGGGAACCTT GAGAGCTCCT ATGGCAATGA AGGCAAAGAG 
TACGTTAAAT GGGCGGCTAG CATGGCTCTA AGTCTTGGTG CTGGTGTTCC ATGGGTCATG 
TGCAAGCAAC CTGATGCTCC ATATGATATT ATTGATACAT GCAATGCATA CTACTGTGAT 
GGATTTAAAC CAAACTCTCG TAATAAACCA ATTTTTTGGA CAGAGAACTG GGATGGATGG 
TACACACAGT GGGGTGAAAG ATTGCCTCAT AGACCTGTTG AAGACCTTGC ATTTGCTGTT 
GCGCGTTTTT TCCAGCGTGG AGGAAGTCTT CAAAATTATT ATATGTATTT TGGTGGAACA 
AATTTTGGCC GCACTGCAGG GGGACCCTTG CAAATTACAA GTTATGATTA TGATGCTCCA 
ATTGATGAGT ATGGTCTGCT GAATGAGCCA AAATGGGGTC ACTTGAAAGA TTTGCATGCG 
GCTTTAAAGC TTTGTGAGCC TGCTCTAGTG GCCGCTGACT CACCAACATA TATAAAACTG 
GGTTCAAAGC AGGAGGCACA TGTATACCAG GAAAATGTCC ACCGTGAAGG ATTGAACTTG 
TCTATATCTC AAATCTCTAA CAAATGCTCA GCGTTCCTTG CCAACATTGA TGAACGTAAA 
GCAGCAACTG TGACCTTTCG TGGTCAGACA TACACTTTAC CACCATGGTC TGTGAGCATA 
TTGCCAGATT GCAGGAGTGC GATTTTCAAT ACTGCTAAGG TTGGAGCACA AACTTCAGTC 
AAACTGGTAG GGTCCAATTT GCCACTCACA TCTAATCTTT TGCTATCCCA GCAATCGATA 
GATCACAATG GCATCTCCCA TATCTCAAAG TCTTGGATGA CTACAAAAGA GCCGATCAAT 
ATATGGATTA ACAGCAGTTT CACTGCAGAA GGTATATGGG AGCACTTAAA TGTAACAAAA 
GATCAATCTG ACTATCTTTG GTATTCAACC AGAATATATG TTTCAGATGG TGATATCTTG 
TTTTGGAAAG AAAATGCTGC TCATCCAAAA CTTGCTATAG ACAGTGTCCG TGATATACTG 
CGTGTATTTG TGAATGGCCA ACTTATAGGT AATGTTGTTG GCCATTGGGT CAAGGCGGTC 
CAGACTCTTC AATTTCAGCC AGGCTACAAT GATTTAACAC TGTTAACTCA AACAGTAGGC 
TTGCAGAATT ACGGTGCCTT CATTGAGAAA GATGGAGCTG GAATAAGAGG TACAATCAAG 
ATCACTGGAT TTGAAAATGG GCACATAGAT CTTTCGAAAC CATTATGGAC CTACCAGGTT 
GGGCTTCAAG GTGAGTTTTT AAAATTTTAC AATGAAGAGA GTGAAAATGC AGGATGGGTA 
GAATTGACTC CTGATGCCAT TCCATCCACG TTTACATGGT ACAAGACATA CTTTGATGTC 
CCTGGTGGAA ATGATCCAGT TGCTCTTGAC TTGGAAAGCA TGGGGAAAGG TCAAGCTTGG 
GTGAATGGTC ACCATATCGG AAGATATTGG ACCCGGGTTT CCCCAAAAAC TGGATGCCAA 
GTCTGTGATT ATCGCGGGGC TTACGATTCT GACAAGTGCA CAACCAATTG TGGAAAACCA 
ACTCAAACTT TGTACCATGT GCCACGATCA TGGTTGAAAG CATCCAACAA TTTCCTTGTT 
ATCTTGGAGG AAACTGGAGG AAATCCCTTA GGAATTTCTG TCAAGTTACA TTCAGCCAGT 
ATAGTTTGTG CTCAAGTGTC ACAATCCTAC TATCCACCTA TGCAGAAGTT GTTGAATGCA 
AGTCTCCTTG GTCAACAAGA AGTTTCTTCA AATGATATGA TACCTGAAAT GAACTTGCGC 
TGTCGAGATG GAAATATAAT TTCCTCCATA ACATTTGCTA GCTTTGGAAC TCCTGGAGGT 
AGTTGTCAGA GTTTTTCTAG AGGGAACTGT CATGCTCCTA GTTCAAAATC TATAGTTTCC 
AAGGCTTGCC TGGGGAAAAG GAGCTGCTCC ATTAAAATTT CAAGTGATGT ATTCGGAGGT 
GACCCCTGCC AGGATGTTGT GAAAACATTG TCTGTGGAAG CAAGGTGCAT CACCATCACT 
AATGGTTCCT TCCAACTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
BMC genomics15312(2014 Apr)
Tang H,Krishnakumar V,Bidwell S,Rosen B,Chan A,Zhou S,Gentzbittel L,Childs KL,Yandell M,Gundlach H,Mayer KF,Schwartz DC,Town CD

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Nature480(7378)520-4(2011 Nov)
Young ND,Debell? F,Oldroyd GE,Geurts R,Cannon SB,Udvardi MK,Benedito VA,Mayer KF,Gouzy J,Schoof H,Van de Peer Y,Proost S,Cook DR,Meyers BC,Spannagl M,Cheung F,De Mita S,Krishnakumar V,Gundlach H,Zhou S,Mudge J,Bharti AK,Murray JD,Naoumkina MA,Rosen B,Silverstein KA,Tang H,Rombauts S,Zhao PX,Zhou P,Barbe V,Bardou P,Bechner M,Bellec A,Berger A,Berg?s H,Bidwell S,Bisseling T,Choisne N,Couloux A,Denny R,Deshpande S,Dai X,Doyle JJ,Dudez AM,Farmer AD,Fouteau S,Franken C,Gibelin C,Gish J,Goldstein S,Gonz?lez AJ,Green PJ,Hallab A,Hartog M,Hua A,Humphray SJ,Jeong DH,Jing Y,J?cker A,Kenton SM,Kim DJ,Klee K,Lai H,Lang C,Lin S,Macmil SL,Magdelenat G,Matthews L,McCorrison J,Monaghan EL,Mun JH,Najar FZ,Nicholson C,Noirot C,O'Bleness M,Paule CR,Poulain J,Prion F,Qin B,Qu C,Retzel EF,Riddle C,Sallet E,Samain S,Samson N,Sanders I,Saurat O,Scarpelli C,Schiex T,Segurens B,Severin AJ,Sherrier DJ,Shi R,Sims S,Singer SR,Sinharoy S,Sterck L,Viollet A,Wang BB,Wang K,Wang M,Wang X,Warfsmann J,Weissenbach J,White DD,White JD,Wiley GB,Wincker P,Xing Y,Yang L,Yao Z,Ying F,Zhai J,Zhou L,Zuber A,D?nari? J,Dixon RA,May GD,Schwartz DC,Rogers J,Qu?tier F,Town CD,Roe BA

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MTR_8g085210 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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