tsc2 gene cDNA ORF clone, Maylandia zebra(zebra mbuna)

mRNA	Protein	Name
XM_004539040.1	XP_004539097.1	tuberin isoform X3
XM_004539036.3	XP_004539093.1	tuberin isoform X1
XM_004539037.2	XP_004539094.1	tuberin isoform X1
XM_004539038.5	XP_004539095.2	tuberin isoform X1
XM_004539039.2	XP_004539096.1	tuberin isoform X2
XM_004539036.2	XP_004539093.1	tuberin isoform X1
XM_004539038.3	XP_004539095.1	tuberin isoform X1
XM_004539038.4	XP_004539095.1	tuberin isoform X1
XM_004539040.2	XP_004539097.1	tuberin isoform X3
XM_004539036.4	XP_004539093.2	tuberin isoform X1
XM_004539037.3	XP_004539094.2	tuberin isoform X1
XM_004539039.4	XP_004539096.1	tuberin isoform X2
XM_004539039.3	XP_004539096.1	tuberin isoform X2
XM_004539040.3	XP_004539097.2	tuberin isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMa18055	XM_004539036.3 Latest version!	Maylandia zebra TSC complex subunit 2 (tsc2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMa18055	XM_004539037.2 Latest version!	Maylandia zebra TSC complex subunit 2 (tsc2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMa18055	XM_004539038.4 Latest version!	Maylandia zebra TSC complex subunit 2 (tsc2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMa18056	XM_004539039.3 Latest version!	Maylandia zebra TSC complex subunit 2 (tsc2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMa18057	XM_004539040.2 Latest version!	Maylandia zebra TSC complex subunit 2 (tsc2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMa374612	XM_004539038.5 Latest version!	Maylandia zebra TSC complex subunit 2 (tsc2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMa374612	XM_004539036.4 Latest version!	Maylandia zebra TSC complex subunit 2 (tsc2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMa374612	XM_004539037.3 Latest version!	Maylandia zebra TSC complex subunit 2 (tsc2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMa374613	XM_004539039.4 Latest version!	Maylandia zebra TSC complex subunit 2 (tsc2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMa374614	XM_004539040.3 Latest version!	Maylandia zebra TSC complex subunit 2 (tsc2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OMa18055	XM_004539036.2	Maylandia zebra tuberous sclerosis 2 (tsc2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMa18055	XM_004539038.3	Maylandia zebra tuberous sclerosis 2 (tsc2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMa18057	XM_004539040.1	Maylandia zebra tuberous sclerosis 2 (tsc2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMa18056	XM_004539039.2	Maylandia zebra tuberous sclerosis 2 (tsc2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

tsc2 ( XM_004539036.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004539093.1 Maylandia zebra TSC complex subunit 2 (tsc2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMa18055
Clone ID Related Accession (Same CDS sequence)	XM_004539036.3 , XM_004539037.2 , XM_004539036.2 , XM_004539038.3 , XM_004539038.4
Accession Version	XM_004539036.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-29
Organism	Maylandia zebra(zebra mbuna)
Product	tuberin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014446893.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 30, 2017 this sequence version replaced XM_004539036.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAAAC AACCAAGCAA GGAGAGTCTC AAGGACAAGG TGAAGGGGAT CTTTGGGCTC 
GGTACACAGC GTCCTCCGAA CAAACAGAGT GACACTAAAC CCTCCGAGTT TATCATTACG 
GCTGACATCA TCAAGGAGCT CCACCCAGAT TGCGGTCTGA GCAACCGTGT TCGCACTATG 
AACCACATCT GTGAGCTTGC CAAAATGAAA AAGTTTGAGG AGCATGCAGT GGAGGCTGTA 
TGGAAAAATG TGGAAGACAT GCTGACTCCA GAGCAGCCAC CAGAGGCCAG ACATGCTGTC 
CTGCAGCTGC TCAGGGCCAT TATACAGGGG CAGGGTGAGC GACTCGGCCC TCTGAGAGCC 
TACTTTTTCA AGGTAATCAG AGACTACCAG CCGTGCAATG AAGACCTTTC TGACAGACTG 
GAGGTGTTTA AGGCTTTAAC AGAGAATGGA AAGGATATCA CATACCTGGA AGAGGACATA 
GCACGCTTTG TCCTCCTGTG GATGGATATA GGCCTTACAT CTGACTTTCT CCATGTTCTT 
GTTAATCTGG TGAAATTCAA TAGCTGCTAC TTGGACCAAA ATGTTTCCAT CATGGTCCAG 
AAAATTTGCC TGCTGTGCAA CAGAACGACG TCTTCGACTG ATATTGAGGT GGCTCTGCAA 
GTGCTCGATG CAGTTGTGTG TTACAACTGC CTGCCCTCCG ACTCTTTAAC CGTTTTCATA 
ATCACACTCT GCCGTACGGT GAACGTCAAA GAGTTTTGCG AGTCCTGTTG GAAGTTAATG 
AGAAAGGTTT TGGGGACTCA CCTCGGCCAT AGTGCAATTT ACACCATGTG TCGCATTATG 
GAGGAAAGGG TGTATATGGA GGATGCACCG TTGTTAAGAG GAGCAGTTTT CTTTGTTGGA 
ATGGCGCTTT GGGGCGCACA CAGACTGCCT GCACTCAAAA ATACACCGAC GCTTGTTCTG 
CCATCTTTCT ACAAGGCCAT GTCATGTGCC AACGAGGTGG TCTCCTACGA AATTGTTCTT 
TCAATAACAA GACTCATCAA AAAGTACGGT AAAGAGCTAC AAGTGGTCAC ATGGGACATC 
CTACTCGGCA TCATAGAGCG ACTGTTGCAA CAGATTCAGA CAATAGGCAG TGCGGAACTA 
AAGGCCATTG TTTATGAACT GTTGACCACC GTGGAAGAGC TTTATGAACA AAACGGTTAC 
CACGGCTCCA CTGAGAAGTT TTTCAGCCTG GTGGAGAAAT GTGCTGATAA AAGACCAGAT 
GCATCAGTGC TGACACTTAT CTCCTACAGG GCTCAGTCTA TCCAGCCTGC TAAAGAAGGC 
TGGATTCAGA GCCTTCACCG GCTCATGGAG AAATTCTTCA GGAACGAGAC TCGCAGTGTG 
ATAAGAATCA AAGTGCTTCA TATCCTGTCC TTCGTTCTCA GTACCAACCG GCAGCTCTAT 
GAGGATGAGT TGATTGAAAT GGTGGTGATT CCCCAGCTCA GTGGGATTGC TGAAGACCGG 
GACTTGGCAG TTAGAAAACA GGCTACTCAG CTATTAGTAG ACCTCGCTGA GGGCTGCAAC 
ACGCACCACT TCACCAGCCT GCTGGACATC ATTGAACGGG TGTCTAGCCG CTCTCTGGTG 
TGTTCAGGGC CCCTAGAGGT TTCTGAGAGA GACCCCACAG CAGAGTCTCC TATGGAGGAC 
GTCAGGACTG CTATTCTGGG CCTGCTTGAA ATCCTGCAGA GCAAACTTTA CAGTCTGCCA 
GCCAGCCATG CGAGTCGTGT TTATGAGTTG CTTATCAGCC ACCTGCAGCT TCATTACAAG 
AACAAGTACT GTTCAACTAT CGCCTCCACT ATTAGACTAA AGGTGTTTGA CTTCTTCCTC 
ATGATGCGTG CAGACTCTCT TCACCGTCTT GGGGTCCCAA ACAAAGATGG TGTCATGAGA 
TTCAGCCCTT ATTGCTACTG TGATATAGGG GAACCAGAGA AACGTGTTGG TGAAAAGAAG 
CCAGCAGGTT CAACCTCTCC TCCTGCTGGC AGCCCTGCTC CTCCTGCTGC TCCTCCCAGT 
TCAGCAGCCT CAACACGCTC AGCCTACCTT TCCTATTCGC CTGCCTTCAG TGTCCTCCTG 
CAGTGCCTCA AGATGGAGAC GGATTGGAAG GTGCTGAAGC TGGTTCTTGA CAAGCTGCCA 
TGGATGCTGC AGTACAAAGT GTTGCTGCTC ACATCACCAT GCAACTTAGA TCAGCTGTGT 
TCTACACTCT GCTGCATGGT GACAGATCGT CTCATCTCAG AGCGATTGAA AAAGACTCCA 
GAGGGGTTTT CTCGCACAGA TGTTCAGCTG GCAGTGATTC CCGTTCTTAC AGCAATTACC 
TCCTACCACA ACTACTTGGA GCAGTCAAGA CAGAGAGAAC TGGTTCAGTG CCTCGAGACT 
GGGCTCATTT ACCGCTGTGC CAAGCAATGT GTGGTGGCCC TTACAATGTG CACGGTGGAG 
ATGCCTGATA TCATGATCAA GCTTCTTCCT GCTCTTATCG TCAAGCTCAC TCACATCTCT 
GCTACTGTTG CCATGGCATC TCCTATGCTC GAGTTTCTCT CCACCCTCGT GCGCCTTCCC 
CATCTGTATG CCAACTTTGT AGCTGAGCAG TATGTCAGCG TGTTTGCCAT CTCACTACCT 
TACACTAACC CCTCAAAGTT CAACCAGTAC ATTGTGTCCC TGGCCCACCA TGTGATTGCC 
ATGTGGTTCA TACGCTGCAG ACTACCCTTC CGCAAGGACT TTGTTCAGTA TATCACAAAG 
GGTTTGCGTT CCAACGCCCT TCTGCCATTC GATGACAGTC ATGAGCAGAG TCCGTTTCGT 
GCCCGAAGCA CCAGCCTCAA TGAGAGACCC AAGAGCTTGC GGGCAGCAAA AGTGGCAAAG 
GCAGCAGCAG CGGTAGCCAA TAGCAGCAGC TCTCCAGTTA AAGAGCTGAG GGACCTTTCA 
GCCATGGACG CTTTCCGCTC CCGCAGCATC AGCGTCTCCG AACATGCGGT CCGCAGGATG 
CACACCTCAA CCACCACATG CAGTCTGGGC TCTGCCGATG AAAATGCAGT AACTCAGGCC 
GACGAGGGGC TGAAAACTGT CCACCTAGAG CTCACAGAGA CTTGTCTTGA CATGATGGCC 
CGATATGTAT TTTCCAACTT CTCTGCACTT CCCAAGAGGT CTCCCATCGC AGAGTTCCTG 
TTAGCAGGAG GCCGCAGTAT GACCTGGTTG GTGGGCAACA AGTTGGTCAC CATAACAACC 
AGCGGAGGGG TCAGAACACA AGCGCTGCTG GGCCTGGACA TGGCTGATCG CCTGGGAGGA 
GGAGGAGGGG AATTGACCAG GTCAGACCCA TCACTACACA CCCGAATGAC CAAAGAGGCT 
CCAGCCAAAT TGGAGTCACA GTCAAGCCAG CAGCACAACA GAGCTGCACG CATACGAGTT 
CGCTCCATGT CAGGTGGTCA TGCTCTACGT GCCGGTCCAG CTCAGAGTCT CAGTCCTCTG 
GTGTCTCCCT CTGAGGGTGA GTTGACTGCT CCACTCTCTC CCTCCTCTGG TCCTGCTCTG 
GATAGTTTAG GTCCATCCTC TTCCACCTCT GCCACTCCAT CTGTGCCTCC TACTCTGAAA 
GACAACCCCA CCTTGGCTGA ATTTGCCCCA ATGCTCACAC AAGGCTGGGC AGAGATCTTC 
ATACGCAGAC CGTCAGGTAA CACAAGCTGG CTAATGTGTT TGGAGAACCC ACCTAGCCCT 
TTCTCATCTG AGCTTGGCAA CATGCCGCTG CAAGAGCTCT CCAGTGTTCT GATGGCAATG 
GAGGGAGTGA AGGAACCTCC CACTCAGACA GCCAGTGCTC CTGCTAGCAC AGCTGTCCCT 
GCTCCAGCTC CTGTCTCTGA GCCCCTAACC CAAACACACA GCAGTGTTGG AGGAAAGGCA 
AACCTGATTC AGCGCTCCAA CACAGTGGGT GGCTCTCTCT GCTCTCTGGG TATGGGCACT 
GCCCCCGCAG GCCCACCAGC CCCTGGCAGG TTGCACAGGA GCATTTCCTG GGCAGACTCT 
GTCGTGGTGC TGGAGGAGGG ATCAGGAATT GCTACAGCAA ACACGCCGCC TGAATGGCTG 
GGGAGTGCAG AGTTTGAGCC AATGTTGTCT GATCCAATCT TCATAGCTGC TGATAAGTTT 
ACAAAGACTC CTCCTGAAAC ACTCAGCAGG TCCTCCTCCA CCTCAAGTCA GGATGATGAA 
AAGTCAACCC TGGAAGAAGT AAGTGAGGGA GCAATTCCAA TCGATCAGCC CCCTTTTGGA 
CCATCCACTC CAGGAAGCCA GGGCCCTGAC CTTCCCTTCC AGACACACAC TCAGTCCCAG 
GGTCATGGAC TCAACAAGTC CAGCTCCTCT CCTGAGCTCC AGACATTACC TGAAGCTTTT 
GCCAAGGCTG CTATGGAGTC AGAAAGTGCG ATGGTTGACA CGTCACGGCC TAGAGCACCC 
TCAGACGGCA TGCCTCAACC CCAGCAGCCC CAGTTTGCGA AAGAAAAGGT AGAGGGAAAC 
ACTGGAGTGG ACACTAATGT AGCGGGAGGT CCAAGCCCAG CAGGTGAAGG CCAAGCTGTA 
TCGTCATCTC AGAGTGGAGG AGCGGGGATG AGGTTGTCTT TTCCACCAGC ACCAGTACAA 
TCTGCACCCA TCTCCCCCAG TGGAGGCCAC CGGCCCAGAG GTCACACAAT CTCAGTCTCA 
GCCCCCTCCT CCAGGAGGGA GAGGCGGGCG GAGAGGGACG CTTACCACAG TCGACCTGGT 
CCCAGCAACA CTGAGAAAAT CTCTGGACTG AGCCCCAGCT TTGTGTTTCT GCAGCTCTAC 
CACTCTCCTT TCTTTGGGAA TGAAGCCAAC AAGCCACTTC TGCTGCCCAA AACTCAGGTG 
ATTGACCGTG CAGTAAAGGT TCTGGACCAA ATGCCCCCTT ATGACACCCA TAAAATTGGG 
GTGGTGTTTG TAGGAGCTGG TCAGGTTAAC AATGAAGTCG CCATCTTGTC GAACGAGTAC 
GGTTCGAACC GCTACGCCTC TTTCCTCACA GGTCTTGGCA AATTAATCCA TCTGAAAGAC 
TGCGATCCAG ACCAGATTTT TCTTGGAGGG CTTGACCAGT ATGGAGATGA TGGAGAGTTC 
ACCTACTGCT GGCATGACGA CATCATGCAG GCTATCTTTC ACATAGCCAC ACTGATGCCA 
AACAGGGAGA GCGACAAGGG TTGCTGCAAC AAAAAGCGAC ACATTGGCAA CGATTTTGTC 
ATGGTGGTGT ACAATGACTC TGGAGAGGAG TACAAACTGG GCACAATTAA GGGTCAGTTT 
AATTTTGTCG AAGTCATTAT AAAACCACTG GATTATGAAT GTAACCTCGT TACCCTCCAG 
TGCCGCAAAG ACCTCGAGGG ATTAGTAGAC ACAACGGTGG CAAAAATTGT TTCAGATCGA 
AACCTTCCCC TGTTAGTCAG GCAGATGGCT CTGCATGCAA ATATGGCCTC ATTGGTGCAC 
CAGTACAGAG CTAACCCCTC TGACGCATAC GCCTCTAAGT GGCTAGCAAG GTTACGACAC 
ATCAAGAGGA TCAGGACCAG GGCTCAAGAA GCCATCCAGT CTCGCTCAAC TCCCGGGATT 
TCACTGACCC AAGGGCAGAG CAAACCTTTG TCACAGAGCA GTGCTCCAAC AAATGTGGAA 
GCCACCGGAC AACGAAAAAG ACTGGTGTCC ACTGTAGACG ATTTCACAGA TTTTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004539093.1
CDS	198..5897
Translation	MNKQPSKESLKDKVKGIFGLGTQRPPNKQSDTKPSEFIITADIIKELHPDCGLSNRVRTMNHICELAKMKKFEEHAVEAVWKNVEDMLTPEQPPEARHAVLQLLRAIIQGQGERLGPLRAYFFKVIRDYQPCNEDLSDRLEVFKALTENGKDITYLEEDIARFVLLWMDIGLTSDFLHVLVNLVKFNSCYLDQNVSIMVQKICLLCNRTTSSTDIEVALQVLDAVVCYNCLPSDSLTVFIITLCRTVNVKEFCESCWKLMRKVLGTHLGHSAIYTMCRIMEERVYMEDAPLLRGAVFFVGMALWGAHRLPALKNTPTLVLPSFYKAMSCANEVVSYEIVLSITRLIKKYGKELQVVTWDILLGIIERLLQQIQTIGSAELKAIVYELLTTVEELYEQNGYHGSTEKFFSLVEKCADKRPDASVLTLISYRAQSIQPAKEGWIQSLHRLMEKFFRNETRSVIRIKVLHILSFVLSTNRQLYEDELIEMVVIPQLSGIAEDRDLAVRKQATQLLVDLAEGCNTHHFTSLLDIIERVSSRSLVCSGPLEVSERDPTAESPMEDVRTAILGLLEILQSKLYSLPASHASRVYELLISHLQLHYKNKYCSTIASTIRLKVFDFFLMMRADSLHRLGVPNKDGVMRFSPYCYCDIGEPEKRVGEKKPAGSTSPPAGSPAPPAAPPSSAASTRSAYLSYSPAFSVLLQCLKMETDWKVLKLVLDKLPWMLQYKVLLLTSPCNLDQLCSTLCCMVTDRLISERLKKTPEGFSRTDVQLAVIPVLTAITSYHNYLEQSRQRELVQCLETGLIYRCAKQCVVALTMCTVEMPDIMIKLLPALIVKLTHISATVAMASPMLEFLSTLVRLPHLYANFVAEQYVSVFAISLPYTNPSKFNQYIVSLAHHVIAMWFIRCRLPFRKDFVQYITKGLRSNALLPFDDSHEQSPFRARSTSLNERPKSLRAAKVAKAAAAVANSSSSPVKELRDLSAMDAFRSRSISVSEHAVRRMHTSTTTCSLGSADENAVTQADEGLKTVHLELTETCLDMMARYVFSNFSALPKRSPIAEFLLAGGRSMTWLVGNKLVTITTSGGVRTQALLGLDMADRLGGGGGELTRSDPSLHTRMTKEAPAKLESQSSQQHNRAARIRVRSMSGGHALRAGPAQSLSPLVSPSEGELTAPLSPSSGPALDSLGPSSSTSATPSVPPTLKDNPTLAEFAPMLTQGWAEIFIRRPSGNTSWLMCLENPPSPFSSELGNMPLQELSSVLMAMEGVKEPPTQTASAPASTAVPAPAPVSEPLTQTHSSVGGKANLIQRSNTVGGSLCSLGMGTAPAGPPAPGRLHRSISWADSVVVLEEGSGIATANTPPEWLGSAEFEPMLSDPIFIAADKFTKTPPETLSRSSSTSSQDDEKSTLEEVSEGAIPIDQPPFGPSTPGSQGPDLPFQTHTQSQGHGLNKSSSSPELQTLPEAFAKAAMESESAMVDTSRPRAPSDGMPQPQQPQFAKEKVEGNTGVDTNVAGGPSPAGEGQAVSSSQSGGAGMRLSFPPAPVQSAPISPSGGHRPRGHTISVSAPSSRRERRAERDAYHSRPGPSNTEKISGLSPSFVFLQLYHSPFFGNEANKPLLLPKTQVIDRAVKVLDQMPPYDTHKIGVVFVGAGQVNNEVAILSNEYGSNRYASFLTGLGKLIHLKDCDPDQIFLGGLDQYGDDGEFTYCWHDDIMQAIFHIATLMPNRESDKGCCNKKRHIGNDFVMVVYNDSGEEYKLGTIKGQFNFVEVIIKPLDYECNLVTLQCRKDLEGLVDTTVAKIVSDRNLPLLVRQMALHANMASLVHQYRANPSDAYASKWLARLRHIKRIRTRAQEAIQSRSTPGISLTQGQSKPLSQSSAPTNVEATGQRKRLVSTVDDFTDFV

Target ORF information:

RefSeq Version	XM_004539036.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra TSC complex subunit 2 (tsc2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004539036.3

ORF Insert Sequence:

ATGAATAAAC AACCAAGCAA GGAGAGTCTC AAGGACAAGG TGAAGGGGAT CTTTGGGCTC 
GGTACACAGC GTCCTCCGAA CAAACAGAGT GACACTAAAC CCTCCGAGTT TATCATTACG 
GCTGACATCA TCAAGGAGCT CCACCCAGAT TGCGGTCTGA GCAACCGTGT TCGCACTATG 
AACCACATCT GTGAGCTTGC CAAAATGAAA AAGTTTGAGG AGCATGCAGT GGAGGCTGTA 
TGGAAAAATG TGGAAGACAT GCTGACTCCA GAGCAGCCAC CAGAGGCCAG ACATGCTGTC 
CTGCAGCTGC TCAGGGCCAT TATACAGGGG CAGGGTGAGC GACTCGGCCC TCTGAGAGCC 
TACTTTTTCA AGGTAATCAG AGACTACCAG CCGTGCAATG AAGACCTTTC TGACAGACTG 
GAGGTGTTTA AGGCTTTAAC AGAGAATGGA AAGGATATCA CATACCTGGA AGAGGACATA 
GCACGCTTTG TCCTCCTGTG GATGGATATA GGCCTTACAT CTGACTTTCT CCATGTTCTT 
GTTAATCTGG TGAAATTCAA TAGCTGCTAC TTGGACCAAA ATGTTTCCAT CATGGTCCAG 
AAAATTTGCC TGCTGTGCAA CAGAACGACG TCTTCGACTG ATATTGAGGT GGCTCTGCAA 
GTGCTCGATG CAGTTGTGTG TTACAACTGC CTGCCCTCCG ACTCTTTAAC CGTTTTCATA 
ATCACACTCT GCCGTACGGT GAACGTCAAA GAGTTTTGCG AGTCCTGTTG GAAGTTAATG 
AGAAAGGTTT TGGGGACTCA CCTCGGCCAT AGTGCAATTT ACACCATGTG TCGCATTATG 
GAGGAAAGGG TGTATATGGA GGATGCACCG TTGTTAAGAG GAGCAGTTTT CTTTGTTGGA 
ATGGCGCTTT GGGGCGCACA CAGACTGCCT GCACTCAAAA ATACACCGAC GCTTGTTCTG 
CCATCTTTCT ACAAGGCCAT GTCATGTGCC AACGAGGTGG TCTCCTACGA AATTGTTCTT 
TCAATAACAA GACTCATCAA AAAGTACGGT AAAGAGCTAC AAGTGGTCAC ATGGGACATC 
CTACTCGGCA TCATAGAGCG ACTGTTGCAA CAGATTCAGA CAATAGGCAG TGCGGAACTA 
AAGGCCATTG TTTATGAACT GTTGACCACC GTGGAAGAGC TTTATGAACA AAACGGTTAC 
CACGGCTCCA CTGAGAAGTT TTTCAGCCTG GTGGAGAAAT GTGCTGATAA AAGACCAGAT 
GCATCAGTGC TGACACTTAT CTCCTACAGG GCTCAGTCTA TCCAGCCTGC TAAAGAAGGC 
TGGATTCAGA GCCTTCACCG GCTCATGGAG AAATTCTTCA GGAACGAGAC TCGCAGTGTG 
ATAAGAATCA AAGTGCTTCA TATCCTGTCC TTCGTTCTCA GTACCAACCG GCAGCTCTAT 
GAGGATGAGT TGATTGAAAT GGTGGTGATT CCCCAGCTCA GTGGGATTGC TGAAGACCGG 
GACTTGGCAG TTAGAAAACA GGCTACTCAG CTATTAGTAG ACCTCGCTGA GGGCTGCAAC 
ACGCACCACT TCACCAGCCT GCTGGACATC ATTGAACGGG TGTCTAGCCG CTCTCTGGTG 
TGTTCAGGGC CCCTAGAGGT TTCTGAGAGA GACCCCACAG CAGAGTCTCC TATGGAGGAC 
GTCAGGACTG CTATTCTGGG CCTGCTTGAA ATCCTGCAGA GCAAACTTTA CAGTCTGCCA 
GCCAGCCATG CGAGTCGTGT TTATGAGTTG CTTATCAGCC ACCTGCAGCT TCATTACAAG 
AACAAGTACT GTTCAACTAT CGCCTCCACT ATTAGACTAA AGGTGTTTGA CTTCTTCCTC 
ATGATGCGTG CAGACTCTCT TCACCGTCTT GGGGTCCCAA ACAAAGATGG TGTCATGAGA 
TTCAGCCCTT ATTGCTACTG TGATATAGGG GAACCAGAGA AACGTGTTGG TGAAAAGAAG 
CCAGCAGGTT CAACCTCTCC TCCTGCTGGC AGCCCTGCTC CTCCTGCTGC TCCTCCCAGT 
TCAGCAGCCT CAACACGCTC AGCCTACCTT TCCTATTCGC CTGCCTTCAG TGTCCTCCTG 
CAGTGCCTCA AGATGGAGAC GGATTGGAAG GTGCTGAAGC TGGTTCTTGA CAAGCTGCCA 
TGGATGCTGC AGTACAAAGT GTTGCTGCTC ACATCACCAT GCAACTTAGA TCAGCTGTGT 
TCTACACTCT GCTGCATGGT GACAGATCGT CTCATCTCAG AGCGATTGAA AAAGACTCCA 
GAGGGGTTTT CTCGCACAGA TGTTCAGCTG GCAGTGATTC CCGTTCTTAC AGCAATTACC 
TCCTACCACA ACTACTTGGA GCAGTCAAGA CAGAGAGAAC TGGTTCAGTG CCTCGAGACT 
GGGCTCATTT ACCGCTGTGC CAAGCAATGT GTGGTGGCCC TTACAATGTG CACGGTGGAG 
ATGCCTGATA TCATGATCAA GCTTCTTCCT GCTCTTATCG TCAAGCTCAC TCACATCTCT 
GCTACTGTTG CCATGGCATC TCCTATGCTC GAGTTTCTCT CCACCCTCGT GCGCCTTCCC 
CATCTGTATG CCAACTTTGT AGCTGAGCAG TATGTCAGCG TGTTTGCCAT CTCACTACCT 
TACACTAACC CCTCAAAGTT CAACCAGTAC ATTGTGTCCC TGGCCCACCA TGTGATTGCC 
ATGTGGTTCA TACGCTGCAG ACTACCCTTC CGCAAGGACT TTGTTCAGTA TATCACAAAG 
GGTTTGCGTT CCAACGCCCT TCTGCCATTC GATGACAGTC ATGAGCAGAG TCCGTTTCGT 
GCCCGAAGCA CCAGCCTCAA TGAGAGACCC AAGAGCTTGC GGGCAGCAAA AGTGGCAAAG 
GCAGCAGCAG CGGTAGCCAA TAGCAGCAGC TCTCCAGTTA AAGAGCTGAG GGACCTTTCA 
GCCATGGACG CTTTCCGCTC CCGCAGCATC AGCGTCTCCG AACATGCGGT CCGCAGGATG 
CACACCTCAA CCACCACATG CAGTCTGGGC TCTGCCGATG AAAATGCAGT AACTCAGGCC 
GACGAGGGGC TGAAAACTGT CCACCTAGAG CTCACAGAGA CTTGTCTTGA CATGATGGCC 
CGATATGTAT TTTCCAACTT CTCTGCACTT CCCAAGAGGT CTCCCATCGC AGAGTTCCTG 
TTAGCAGGAG GCCGCAGTAT GACCTGGTTG GTGGGCAACA AGTTGGTCAC CATAACAACC 
AGCGGAGGGG TCAGAACACA AGCGCTGCTG GGCCTGGACA TGGCTGATCG CCTGGGAGGA 
GGAGGAGGGG AATTGACCAG GTCAGACCCA TCACTACACA CCCGAATGAC CAAAGAGGCT 
CCAGCCAAAT TGGAGTCACA GTCAAGCCAG CAGCACAACA GAGCTGCACG CATACGAGTT 
CGCTCCATGT CAGGTGGTCA TGCTCTACGT GCCGGTCCAG CTCAGAGTCT CAGTCCTCTG 
GTGTCTCCCT CTGAGGGTGA GTTGACTGCT CCACTCTCTC CCTCCTCTGG TCCTGCTCTG 
GATAGTTTAG GTCCATCCTC TTCCACCTCT GCCACTCCAT CTGTGCCTCC TACTCTGAAA 
GACAACCCCA CCTTGGCTGA ATTTGCCCCA ATGCTCACAC AAGGCTGGGC AGAGATCTTC 
ATACGCAGAC CGTCAGGTAA CACAAGCTGG CTAATGTGTT TGGAGAACCC ACCTAGCCCT 
TTCTCATCTG AGCTTGGCAA CATGCCGCTG CAAGAGCTCT CCAGTGTTCT GATGGCAATG 
GAGGGAGTGA AGGAACCTCC CACTCAGACA GCCAGTGCTC CTGCTAGCAC AGCTGTCCCT 
GCTCCAGCTC CTGTCTCTGA GCCCCTAACC CAAACACACA GCAGTGTTGG AGGAAAGGCA 
AACCTGATTC AGCGCTCCAA CACAGTGGGT GGCTCTCTCT GCTCTCTGGG TATGGGCACT 
GCCCCCGCAG GCCCACCAGC CCCTGGCAGG TTGCACAGGA GCATTTCCTG GGCAGACTCT 
GTCGTGGTGC TGGAGGAGGG ATCAGGAATT GCTACAGCAA ACACGCCGCC TGAATGGCTG 
GGGAGTGCAG AGTTTGAGCC AATGTTGTCT GATCCAATCT TCATAGCTGC TGATAAGTTT 
ACAAAGACTC CTCCTGAAAC ACTCAGCAGG TCCTCCTCCA CCTCAAGTCA GGATGATGAA 
AAGTCAACCC TGGAAGAAGT AAGTGAGGGA GCAATTCCAA TCGATCAGCC CCCTTTTGGA 
CCATCCACTC CAGGAAGCCA GGGCCCTGAC CTTCCCTTCC AGACACACAC TCAGTCCCAG 
GGTCATGGAC TCAACAAGTC CAGCTCCTCT CCTGAGCTCC AGACATTACC TGAAGCTTTT 
GCCAAGGCTG CTATGGAGTC AGAAAGTGCG ATGGTTGACA CGTCACGGCC TAGAGCACCC 
TCAGACGGCA TGCCTCAACC CCAGCAGCCC CAGTTTGCGA AAGAAAAGGT AGAGGGAAAC 
ACTGGAGTGG ACACTAATGT AGCGGGAGGT CCAAGCCCAG CAGGTGAAGG CCAAGCTGTA 
TCGTCATCTC AGAGTGGAGG AGCGGGGATG AGGTTGTCTT TTCCACCAGC ACCAGTACAA 
TCTGCACCCA TCTCCCCCAG TGGAGGCCAC CGGCCCAGAG GTCACACAAT CTCAGTCTCA 
GCCCCCTCCT CCAGGAGGGA GAGGCGGGCG GAGAGGGACG CTTACCACAG TCGACCTGGT 
CCCAGCAACA CTGAGAAAAT CTCTGGACTG AGCCCCAGCT TTGTGTTTCT GCAGCTCTAC 
CACTCTCCTT TCTTTGGGAA TGAAGCCAAC AAGCCACTTC TGCTGCCCAA AACTCAGGTG 
ATTGACCGTG CAGTAAAGGT TCTGGACCAA ATGCCCCCTT ATGACACCCA TAAAATTGGG 
GTGGTGTTTG TAGGAGCTGG TCAGGTTAAC AATGAAGTCG CCATCTTGTC GAACGAGTAC 
GGTTCGAACC GCTACGCCTC TTTCCTCACA GGTCTTGGCA AATTAATCCA TCTGAAAGAC 
TGCGATCCAG ACCAGATTTT TCTTGGAGGG CTTGACCAGT ATGGAGATGA TGGAGAGTTC 
ACCTACTGCT GGCATGACGA CATCATGCAG GCTATCTTTC ACATAGCCAC ACTGATGCCA 
AACAGGGAGA GCGACAAGGG TTGCTGCAAC AAAAAGCGAC ACATTGGCAA CGATTTTGTC 
ATGGTGGTGT ACAATGACTC TGGAGAGGAG TACAAACTGG GCACAATTAA GGGTCAGTTT 
AATTTTGTCG AAGTCATTAT AAAACCACTG GATTATGAAT GTAACCTCGT TACCCTCCAG 
TGCCGCAAAG ACCTCGAGGG ATTAGTAGAC ACAACGGTGG CAAAAATTGT TTCAGATCGA 
AACCTTCCCC TGTTAGTCAG GCAGATGGCT CTGCATGCAA ATATGGCCTC ATTGGTGCAC 
CAGTACAGAG CTAACCCCTC TGACGCATAC GCCTCTAAGT GGCTAGCAAG GTTACGACAC 
ATCAAGAGGA TCAGGACCAG GGCTCAAGAA GCCATCCAGT CTCGCTCAAC TCCCGGGATT 
TCACTGACCC AAGGGCAGAG CAAACCTTTG TCACAGAGCA GTGCTCCAAC AAATGTGGAA 
GCCACCGGAC AACGAAAAAG ACTGGTGTCC ACTGTAGACG ATTTCACAGA TTTTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tsc2 ( XM_004539037.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004539094.1 Maylandia zebra TSC complex subunit 2 (tsc2), transcript variant X2, mRNA.

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CloneID	OMa18055
Clone ID Related Accession (Same CDS sequence)	XM_004539036.3 , XM_004539037.2 , XM_004539036.2 , XM_004539038.3 , XM_004539038.4
Accession Version	XM_004539037.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-29
Organism	Maylandia zebra(zebra mbuna)
Product	tuberin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014446893.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 17, 2015 this sequence version replaced XM_004539037.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAAAC AACCAAGCAA GGAGAGTCTC AAGGACAAGG TGAAGGGGAT CTTTGGGCTC 
GGTACACAGC GTCCTCCGAA CAAACAGAGT GACACTAAAC CCTCCGAGTT TATCATTACG 
GCTGACATCA TCAAGGAGCT CCACCCAGAT TGCGGTCTGA GCAACCGTGT TCGCACTATG 
AACCACATCT GTGAGCTTGC CAAAATGAAA AAGTTTGAGG AGCATGCAGT GGAGGCTGTA 
TGGAAAAATG TGGAAGACAT GCTGACTCCA GAGCAGCCAC CAGAGGCCAG ACATGCTGTC 
CTGCAGCTGC TCAGGGCCAT TATACAGGGG CAGGGTGAGC GACTCGGCCC TCTGAGAGCC 
TACTTTTTCA AGGTAATCAG AGACTACCAG CCGTGCAATG AAGACCTTTC TGACAGACTG 
GAGGTGTTTA AGGCTTTAAC AGAGAATGGA AAGGATATCA CATACCTGGA AGAGGACATA 
GCACGCTTTG TCCTCCTGTG GATGGATATA GGCCTTACAT CTGACTTTCT CCATGTTCTT 
GTTAATCTGG TGAAATTCAA TAGCTGCTAC TTGGACCAAA ATGTTTCCAT CATGGTCCAG 
AAAATTTGCC TGCTGTGCAA CAGAACGACG TCTTCGACTG ATATTGAGGT GGCTCTGCAA 
GTGCTCGATG CAGTTGTGTG TTACAACTGC CTGCCCTCCG ACTCTTTAAC CGTTTTCATA 
ATCACACTCT GCCGTACGGT GAACGTCAAA GAGTTTTGCG AGTCCTGTTG GAAGTTAATG 
AGAAAGGTTT TGGGGACTCA CCTCGGCCAT AGTGCAATTT ACACCATGTG TCGCATTATG 
GAGGAAAGGG TGTATATGGA GGATGCACCG TTGTTAAGAG GAGCAGTTTT CTTTGTTGGA 
ATGGCGCTTT GGGGCGCACA CAGACTGCCT GCACTCAAAA ATACACCGAC GCTTGTTCTG 
CCATCTTTCT ACAAGGCCAT GTCATGTGCC AACGAGGTGG TCTCCTACGA AATTGTTCTT 
TCAATAACAA GACTCATCAA AAAGTACGGT AAAGAGCTAC AAGTGGTCAC ATGGGACATC 
CTACTCGGCA TCATAGAGCG ACTGTTGCAA CAGATTCAGA CAATAGGCAG TGCGGAACTA 
AAGGCCATTG TTTATGAACT GTTGACCACC GTGGAAGAGC TTTATGAACA AAACGGTTAC 
CACGGCTCCA CTGAGAAGTT TTTCAGCCTG GTGGAGAAAT GTGCTGATAA AAGACCAGAT 
GCATCAGTGC TGACACTTAT CTCCTACAGG GCTCAGTCTA TCCAGCCTGC TAAAGAAGGC 
TGGATTCAGA GCCTTCACCG GCTCATGGAG AAATTCTTCA GGAACGAGAC TCGCAGTGTG 
ATAAGAATCA AAGTGCTTCA TATCCTGTCC TTCGTTCTCA GTACCAACCG GCAGCTCTAT 
GAGGATGAGT TGATTGAAAT GGTGGTGATT CCCCAGCTCA GTGGGATTGC TGAAGACCGG 
GACTTGGCAG TTAGAAAACA GGCTACTCAG CTATTAGTAG ACCTCGCTGA GGGCTGCAAC 
ACGCACCACT TCACCAGCCT GCTGGACATC ATTGAACGGG TGTCTAGCCG CTCTCTGGTG 
TGTTCAGGGC CCCTAGAGGT TTCTGAGAGA GACCCCACAG CAGAGTCTCC TATGGAGGAC 
GTCAGGACTG CTATTCTGGG CCTGCTTGAA ATCCTGCAGA GCAAACTTTA CAGTCTGCCA 
GCCAGCCATG CGAGTCGTGT TTATGAGTTG CTTATCAGCC ACCTGCAGCT TCATTACAAG 
AACAAGTACT GTTCAACTAT CGCCTCCACT ATTAGACTAA AGGTGTTTGA CTTCTTCCTC 
ATGATGCGTG CAGACTCTCT TCACCGTCTT GGGGTCCCAA ACAAAGATGG TGTCATGAGA 
TTCAGCCCTT ATTGCTACTG TGATATAGGG GAACCAGAGA AACGTGTTGG TGAAAAGAAG 
CCAGCAGGTT CAACCTCTCC TCCTGCTGGC AGCCCTGCTC CTCCTGCTGC TCCTCCCAGT 
TCAGCAGCCT CAACACGCTC AGCCTACCTT TCCTATTCGC CTGCCTTCAG TGTCCTCCTG 
CAGTGCCTCA AGATGGAGAC GGATTGGAAG GTGCTGAAGC TGGTTCTTGA CAAGCTGCCA 
TGGATGCTGC AGTACAAAGT GTTGCTGCTC ACATCACCAT GCAACTTAGA TCAGCTGTGT 
TCTACACTCT GCTGCATGGT GACAGATCGT CTCATCTCAG AGCGATTGAA AAAGACTCCA 
GAGGGGTTTT CTCGCACAGA TGTTCAGCTG GCAGTGATTC CCGTTCTTAC AGCAATTACC 
TCCTACCACA ACTACTTGGA GCAGTCAAGA CAGAGAGAAC TGGTTCAGTG CCTCGAGACT 
GGGCTCATTT ACCGCTGTGC CAAGCAATGT GTGGTGGCCC TTACAATGTG CACGGTGGAG 
ATGCCTGATA TCATGATCAA GCTTCTTCCT GCTCTTATCG TCAAGCTCAC TCACATCTCT 
GCTACTGTTG CCATGGCATC TCCTATGCTC GAGTTTCTCT CCACCCTCGT GCGCCTTCCC 
CATCTGTATG CCAACTTTGT AGCTGAGCAG TATGTCAGCG TGTTTGCCAT CTCACTACCT 
TACACTAACC CCTCAAAGTT CAACCAGTAC ATTGTGTCCC TGGCCCACCA TGTGATTGCC 
ATGTGGTTCA TACGCTGCAG ACTACCCTTC CGCAAGGACT TTGTTCAGTA TATCACAAAG 
GGTTTGCGTT CCAACGCCCT TCTGCCATTC GATGACAGTC ATGAGCAGAG TCCGTTTCGT 
GCCCGAAGCA CCAGCCTCAA TGAGAGACCC AAGAGCTTGC GGGCAGCAAA AGTGGCAAAG 
GCAGCAGCAG CGGTAGCCAA TAGCAGCAGC TCTCCAGTTA AAGAGCTGAG GGACCTTTCA 
GCCATGGACG CTTTCCGCTC CCGCAGCATC AGCGTCTCCG AACATGCGGT CCGCAGGATG 
CACACCTCAA CCACCACATG CAGTCTGGGC TCTGCCGATG AAAATGCAGT AACTCAGGCC 
GACGAGGGGC TGAAAACTGT CCACCTAGAG CTCACAGAGA CTTGTCTTGA CATGATGGCC 
CGATATGTAT TTTCCAACTT CTCTGCACTT CCCAAGAGGT CTCCCATCGC AGAGTTCCTG 
TTAGCAGGAG GCCGCAGTAT GACCTGGTTG GTGGGCAACA AGTTGGTCAC CATAACAACC 
AGCGGAGGGG TCAGAACACA AGCGCTGCTG GGCCTGGACA TGGCTGATCG CCTGGGAGGA 
GGAGGAGGGG AATTGACCAG GTCAGACCCA TCACTACACA CCCGAATGAC CAAAGAGGCT 
CCAGCCAAAT TGGAGTCACA GTCAAGCCAG CAGCACAACA GAGCTGCACG CATACGAGTT 
CGCTCCATGT CAGGTGGTCA TGCTCTACGT GCCGGTCCAG CTCAGAGTCT CAGTCCTCTG 
GTGTCTCCCT CTGAGGGTGA GTTGACTGCT CCACTCTCTC CCTCCTCTGG TCCTGCTCTG 
GATAGTTTAG GTCCATCCTC TTCCACCTCT GCCACTCCAT CTGTGCCTCC TACTCTGAAA 
GACAACCCCA CCTTGGCTGA ATTTGCCCCA ATGCTCACAC AAGGCTGGGC AGAGATCTTC 
ATACGCAGAC CGTCAGGTAA CACAAGCTGG CTAATGTGTT TGGAGAACCC ACCTAGCCCT 
TTCTCATCTG AGCTTGGCAA CATGCCGCTG CAAGAGCTCT CCAGTGTTCT GATGGCAATG 
GAGGGAGTGA AGGAACCTCC CACTCAGACA GCCAGTGCTC CTGCTAGCAC AGCTGTCCCT 
GCTCCAGCTC CTGTCTCTGA GCCCCTAACC CAAACACACA GCAGTGTTGG AGGAAAGGCA 
AACCTGATTC AGCGCTCCAA CACAGTGGGT GGCTCTCTCT GCTCTCTGGG TATGGGCACT 
GCCCCCGCAG GCCCACCAGC CCCTGGCAGG TTGCACAGGA GCATTTCCTG GGCAGACTCT 
GTCGTGGTGC TGGAGGAGGG ATCAGGAATT GCTACAGCAA ACACGCCGCC TGAATGGCTG 
GGGAGTGCAG AGTTTGAGCC AATGTTGTCT GATCCAATCT TCATAGCTGC TGATAAGTTT 
ACAAAGACTC CTCCTGAAAC ACTCAGCAGG TCCTCCTCCA CCTCAAGTCA GGATGATGAA 
AAGTCAACCC TGGAAGAAGT AAGTGAGGGA GCAATTCCAA TCGATCAGCC CCCTTTTGGA 
CCATCCACTC CAGGAAGCCA GGGCCCTGAC CTTCCCTTCC AGACACACAC TCAGTCCCAG 
GGTCATGGAC TCAACAAGTC CAGCTCCTCT CCTGAGCTCC AGACATTACC TGAAGCTTTT 
GCCAAGGCTG CTATGGAGTC AGAAAGTGCG ATGGTTGACA CGTCACGGCC TAGAGCACCC 
TCAGACGGCA TGCCTCAACC CCAGCAGCCC CAGTTTGCGA AAGAAAAGGT AGAGGGAAAC 
ACTGGAGTGG ACACTAATGT AGCGGGAGGT CCAAGCCCAG CAGGTGAAGG CCAAGCTGTA 
TCGTCATCTC AGAGTGGAGG AGCGGGGATG AGGTTGTCTT TTCCACCAGC ACCAGTACAA 
TCTGCACCCA TCTCCCCCAG TGGAGGCCAC CGGCCCAGAG GTCACACAAT CTCAGTCTCA 
GCCCCCTCCT CCAGGAGGGA GAGGCGGGCG GAGAGGGACG CTTACCACAG TCGACCTGGT 
CCCAGCAACA CTGAGAAAAT CTCTGGACTG AGCCCCAGCT TTGTGTTTCT GCAGCTCTAC 
CACTCTCCTT TCTTTGGGAA TGAAGCCAAC AAGCCACTTC TGCTGCCCAA AACTCAGGTG 
ATTGACCGTG CAGTAAAGGT TCTGGACCAA ATGCCCCCTT ATGACACCCA TAAAATTGGG 
GTGGTGTTTG TAGGAGCTGG TCAGGTTAAC AATGAAGTCG CCATCTTGTC GAACGAGTAC 
GGTTCGAACC GCTACGCCTC TTTCCTCACA GGTCTTGGCA AATTAATCCA TCTGAAAGAC 
TGCGATCCAG ACCAGATTTT TCTTGGAGGG CTTGACCAGT ATGGAGATGA TGGAGAGTTC 
ACCTACTGCT GGCATGACGA CATCATGCAG GCTATCTTTC ACATAGCCAC ACTGATGCCA 
AACAGGGAGA GCGACAAGGG TTGCTGCAAC AAAAAGCGAC ACATTGGCAA CGATTTTGTC 
ATGGTGGTGT ACAATGACTC TGGAGAGGAG TACAAACTGG GCACAATTAA GGGTCAGTTT 
AATTTTGTCG AAGTCATTAT AAAACCACTG GATTATGAAT GTAACCTCGT TACCCTCCAG 
TGCCGCAAAG ACCTCGAGGG ATTAGTAGAC ACAACGGTGG CAAAAATTGT TTCAGATCGA 
AACCTTCCCC TGTTAGTCAG GCAGATGGCT CTGCATGCAA ATATGGCCTC ATTGGTGCAC 
CAGTACAGAG CTAACCCCTC TGACGCATAC GCCTCTAAGT GGCTAGCAAG GTTACGACAC 
ATCAAGAGGA TCAGGACCAG GGCTCAAGAA GCCATCCAGT CTCGCTCAAC TCCCGGGATT 
TCACTGACCC AAGGGCAGAG CAAACCTTTG TCACAGAGCA GTGCTCCAAC AAATGTGGAA 
GCCACCGGAC AACGAAAAAG ACTGGTGTCC ACTGTAGACG ATTTCACAGA TTTTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004539094.1
CDS	128..5827
Translation	MNKQPSKESLKDKVKGIFGLGTQRPPNKQSDTKPSEFIITADIIKELHPDCGLSNRVRTMNHICELAKMKKFEEHAVEAVWKNVEDMLTPEQPPEARHAVLQLLRAIIQGQGERLGPLRAYFFKVIRDYQPCNEDLSDRLEVFKALTENGKDITYLEEDIARFVLLWMDIGLTSDFLHVLVNLVKFNSCYLDQNVSIMVQKICLLCNRTTSSTDIEVALQVLDAVVCYNCLPSDSLTVFIITLCRTVNVKEFCESCWKLMRKVLGTHLGHSAIYTMCRIMEERVYMEDAPLLRGAVFFVGMALWGAHRLPALKNTPTLVLPSFYKAMSCANEVVSYEIVLSITRLIKKYGKELQVVTWDILLGIIERLLQQIQTIGSAELKAIVYELLTTVEELYEQNGYHGSTEKFFSLVEKCADKRPDASVLTLISYRAQSIQPAKEGWIQSLHRLMEKFFRNETRSVIRIKVLHILSFVLSTNRQLYEDELIEMVVIPQLSGIAEDRDLAVRKQATQLLVDLAEGCNTHHFTSLLDIIERVSSRSLVCSGPLEVSERDPTAESPMEDVRTAILGLLEILQSKLYSLPASHASRVYELLISHLQLHYKNKYCSTIASTIRLKVFDFFLMMRADSLHRLGVPNKDGVMRFSPYCYCDIGEPEKRVGEKKPAGSTSPPAGSPAPPAAPPSSAASTRSAYLSYSPAFSVLLQCLKMETDWKVLKLVLDKLPWMLQYKVLLLTSPCNLDQLCSTLCCMVTDRLISERLKKTPEGFSRTDVQLAVIPVLTAITSYHNYLEQSRQRELVQCLETGLIYRCAKQCVVALTMCTVEMPDIMIKLLPALIVKLTHISATVAMASPMLEFLSTLVRLPHLYANFVAEQYVSVFAISLPYTNPSKFNQYIVSLAHHVIAMWFIRCRLPFRKDFVQYITKGLRSNALLPFDDSHEQSPFRARSTSLNERPKSLRAAKVAKAAAAVANSSSSPVKELRDLSAMDAFRSRSISVSEHAVRRMHTSTTTCSLGSADENAVTQADEGLKTVHLELTETCLDMMARYVFSNFSALPKRSPIAEFLLAGGRSMTWLVGNKLVTITTSGGVRTQALLGLDMADRLGGGGGELTRSDPSLHTRMTKEAPAKLESQSSQQHNRAARIRVRSMSGGHALRAGPAQSLSPLVSPSEGELTAPLSPSSGPALDSLGPSSSTSATPSVPPTLKDNPTLAEFAPMLTQGWAEIFIRRPSGNTSWLMCLENPPSPFSSELGNMPLQELSSVLMAMEGVKEPPTQTASAPASTAVPAPAPVSEPLTQTHSSVGGKANLIQRSNTVGGSLCSLGMGTAPAGPPAPGRLHRSISWADSVVVLEEGSGIATANTPPEWLGSAEFEPMLSDPIFIAADKFTKTPPETLSRSSSTSSQDDEKSTLEEVSEGAIPIDQPPFGPSTPGSQGPDLPFQTHTQSQGHGLNKSSSSPELQTLPEAFAKAAMESESAMVDTSRPRAPSDGMPQPQQPQFAKEKVEGNTGVDTNVAGGPSPAGEGQAVSSSQSGGAGMRLSFPPAPVQSAPISPSGGHRPRGHTISVSAPSSRRERRAERDAYHSRPGPSNTEKISGLSPSFVFLQLYHSPFFGNEANKPLLLPKTQVIDRAVKVLDQMPPYDTHKIGVVFVGAGQVNNEVAILSNEYGSNRYASFLTGLGKLIHLKDCDPDQIFLGGLDQYGDDGEFTYCWHDDIMQAIFHIATLMPNRESDKGCCNKKRHIGNDFVMVVYNDSGEEYKLGTIKGQFNFVEVIIKPLDYECNLVTLQCRKDLEGLVDTTVAKIVSDRNLPLLVRQMALHANMASLVHQYRANPSDAYASKWLARLRHIKRIRTRAQEAIQSRSTPGISLTQGQSKPLSQSSAPTNVEATGQRKRLVSTVDDFTDFV

Target ORF information:

RefSeq Version	XM_004539037.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra TSC complex subunit 2 (tsc2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004539037.2

ORF Insert Sequence:

ATGAATAAAC AACCAAGCAA GGAGAGTCTC AAGGACAAGG TGAAGGGGAT CTTTGGGCTC 
GGTACACAGC GTCCTCCGAA CAAACAGAGT GACACTAAAC CCTCCGAGTT TATCATTACG 
GCTGACATCA TCAAGGAGCT CCACCCAGAT TGCGGTCTGA GCAACCGTGT TCGCACTATG 
AACCACATCT GTGAGCTTGC CAAAATGAAA AAGTTTGAGG AGCATGCAGT GGAGGCTGTA 
TGGAAAAATG TGGAAGACAT GCTGACTCCA GAGCAGCCAC CAGAGGCCAG ACATGCTGTC 
CTGCAGCTGC TCAGGGCCAT TATACAGGGG CAGGGTGAGC GACTCGGCCC TCTGAGAGCC 
TACTTTTTCA AGGTAATCAG AGACTACCAG CCGTGCAATG AAGACCTTTC TGACAGACTG 
GAGGTGTTTA AGGCTTTAAC AGAGAATGGA AAGGATATCA CATACCTGGA AGAGGACATA 
GCACGCTTTG TCCTCCTGTG GATGGATATA GGCCTTACAT CTGACTTTCT CCATGTTCTT 
GTTAATCTGG TGAAATTCAA TAGCTGCTAC TTGGACCAAA ATGTTTCCAT CATGGTCCAG 
AAAATTTGCC TGCTGTGCAA CAGAACGACG TCTTCGACTG ATATTGAGGT GGCTCTGCAA 
GTGCTCGATG CAGTTGTGTG TTACAACTGC CTGCCCTCCG ACTCTTTAAC CGTTTTCATA 
ATCACACTCT GCCGTACGGT GAACGTCAAA GAGTTTTGCG AGTCCTGTTG GAAGTTAATG 
AGAAAGGTTT TGGGGACTCA CCTCGGCCAT AGTGCAATTT ACACCATGTG TCGCATTATG 
GAGGAAAGGG TGTATATGGA GGATGCACCG TTGTTAAGAG GAGCAGTTTT CTTTGTTGGA 
ATGGCGCTTT GGGGCGCACA CAGACTGCCT GCACTCAAAA ATACACCGAC GCTTGTTCTG 
CCATCTTTCT ACAAGGCCAT GTCATGTGCC AACGAGGTGG TCTCCTACGA AATTGTTCTT 
TCAATAACAA GACTCATCAA AAAGTACGGT AAAGAGCTAC AAGTGGTCAC ATGGGACATC 
CTACTCGGCA TCATAGAGCG ACTGTTGCAA CAGATTCAGA CAATAGGCAG TGCGGAACTA 
AAGGCCATTG TTTATGAACT GTTGACCACC GTGGAAGAGC TTTATGAACA AAACGGTTAC 
CACGGCTCCA CTGAGAAGTT TTTCAGCCTG GTGGAGAAAT GTGCTGATAA AAGACCAGAT 
GCATCAGTGC TGACACTTAT CTCCTACAGG GCTCAGTCTA TCCAGCCTGC TAAAGAAGGC 
TGGATTCAGA GCCTTCACCG GCTCATGGAG AAATTCTTCA GGAACGAGAC TCGCAGTGTG 
ATAAGAATCA AAGTGCTTCA TATCCTGTCC TTCGTTCTCA GTACCAACCG GCAGCTCTAT 
GAGGATGAGT TGATTGAAAT GGTGGTGATT CCCCAGCTCA GTGGGATTGC TGAAGACCGG 
GACTTGGCAG TTAGAAAACA GGCTACTCAG CTATTAGTAG ACCTCGCTGA GGGCTGCAAC 
ACGCACCACT TCACCAGCCT GCTGGACATC ATTGAACGGG TGTCTAGCCG CTCTCTGGTG 
TGTTCAGGGC CCCTAGAGGT TTCTGAGAGA GACCCCACAG CAGAGTCTCC TATGGAGGAC 
GTCAGGACTG CTATTCTGGG CCTGCTTGAA ATCCTGCAGA GCAAACTTTA CAGTCTGCCA 
GCCAGCCATG CGAGTCGTGT TTATGAGTTG CTTATCAGCC ACCTGCAGCT TCATTACAAG 
AACAAGTACT GTTCAACTAT CGCCTCCACT ATTAGACTAA AGGTGTTTGA CTTCTTCCTC 
ATGATGCGTG CAGACTCTCT TCACCGTCTT GGGGTCCCAA ACAAAGATGG TGTCATGAGA 
TTCAGCCCTT ATTGCTACTG TGATATAGGG GAACCAGAGA AACGTGTTGG TGAAAAGAAG 
CCAGCAGGTT CAACCTCTCC TCCTGCTGGC AGCCCTGCTC CTCCTGCTGC TCCTCCCAGT 
TCAGCAGCCT CAACACGCTC AGCCTACCTT TCCTATTCGC CTGCCTTCAG TGTCCTCCTG 
CAGTGCCTCA AGATGGAGAC GGATTGGAAG GTGCTGAAGC TGGTTCTTGA CAAGCTGCCA 
TGGATGCTGC AGTACAAAGT GTTGCTGCTC ACATCACCAT GCAACTTAGA TCAGCTGTGT 
TCTACACTCT GCTGCATGGT GACAGATCGT CTCATCTCAG AGCGATTGAA AAAGACTCCA 
GAGGGGTTTT CTCGCACAGA TGTTCAGCTG GCAGTGATTC CCGTTCTTAC AGCAATTACC 
TCCTACCACA ACTACTTGGA GCAGTCAAGA CAGAGAGAAC TGGTTCAGTG CCTCGAGACT 
GGGCTCATTT ACCGCTGTGC CAAGCAATGT GTGGTGGCCC TTACAATGTG CACGGTGGAG 
ATGCCTGATA TCATGATCAA GCTTCTTCCT GCTCTTATCG TCAAGCTCAC TCACATCTCT 
GCTACTGTTG CCATGGCATC TCCTATGCTC GAGTTTCTCT CCACCCTCGT GCGCCTTCCC 
CATCTGTATG CCAACTTTGT AGCTGAGCAG TATGTCAGCG TGTTTGCCAT CTCACTACCT 
TACACTAACC CCTCAAAGTT CAACCAGTAC ATTGTGTCCC TGGCCCACCA TGTGATTGCC 
ATGTGGTTCA TACGCTGCAG ACTACCCTTC CGCAAGGACT TTGTTCAGTA TATCACAAAG 
GGTTTGCGTT CCAACGCCCT TCTGCCATTC GATGACAGTC ATGAGCAGAG TCCGTTTCGT 
GCCCGAAGCA CCAGCCTCAA TGAGAGACCC AAGAGCTTGC GGGCAGCAAA AGTGGCAAAG 
GCAGCAGCAG CGGTAGCCAA TAGCAGCAGC TCTCCAGTTA AAGAGCTGAG GGACCTTTCA 
GCCATGGACG CTTTCCGCTC CCGCAGCATC AGCGTCTCCG AACATGCGGT CCGCAGGATG 
CACACCTCAA CCACCACATG CAGTCTGGGC TCTGCCGATG AAAATGCAGT AACTCAGGCC 
GACGAGGGGC TGAAAACTGT CCACCTAGAG CTCACAGAGA CTTGTCTTGA CATGATGGCC 
CGATATGTAT TTTCCAACTT CTCTGCACTT CCCAAGAGGT CTCCCATCGC AGAGTTCCTG 
TTAGCAGGAG GCCGCAGTAT GACCTGGTTG GTGGGCAACA AGTTGGTCAC CATAACAACC 
AGCGGAGGGG TCAGAACACA AGCGCTGCTG GGCCTGGACA TGGCTGATCG CCTGGGAGGA 
GGAGGAGGGG AATTGACCAG GTCAGACCCA TCACTACACA CCCGAATGAC CAAAGAGGCT 
CCAGCCAAAT TGGAGTCACA GTCAAGCCAG CAGCACAACA GAGCTGCACG CATACGAGTT 
CGCTCCATGT CAGGTGGTCA TGCTCTACGT GCCGGTCCAG CTCAGAGTCT CAGTCCTCTG 
GTGTCTCCCT CTGAGGGTGA GTTGACTGCT CCACTCTCTC CCTCCTCTGG TCCTGCTCTG 
GATAGTTTAG GTCCATCCTC TTCCACCTCT GCCACTCCAT CTGTGCCTCC TACTCTGAAA 
GACAACCCCA CCTTGGCTGA ATTTGCCCCA ATGCTCACAC AAGGCTGGGC AGAGATCTTC 
ATACGCAGAC CGTCAGGTAA CACAAGCTGG CTAATGTGTT TGGAGAACCC ACCTAGCCCT 
TTCTCATCTG AGCTTGGCAA CATGCCGCTG CAAGAGCTCT CCAGTGTTCT GATGGCAATG 
GAGGGAGTGA AGGAACCTCC CACTCAGACA GCCAGTGCTC CTGCTAGCAC AGCTGTCCCT 
GCTCCAGCTC CTGTCTCTGA GCCCCTAACC CAAACACACA GCAGTGTTGG AGGAAAGGCA 
AACCTGATTC AGCGCTCCAA CACAGTGGGT GGCTCTCTCT GCTCTCTGGG TATGGGCACT 
GCCCCCGCAG GCCCACCAGC CCCTGGCAGG TTGCACAGGA GCATTTCCTG GGCAGACTCT 
GTCGTGGTGC TGGAGGAGGG ATCAGGAATT GCTACAGCAA ACACGCCGCC TGAATGGCTG 
GGGAGTGCAG AGTTTGAGCC AATGTTGTCT GATCCAATCT TCATAGCTGC TGATAAGTTT 
ACAAAGACTC CTCCTGAAAC ACTCAGCAGG TCCTCCTCCA CCTCAAGTCA GGATGATGAA 
AAGTCAACCC TGGAAGAAGT AAGTGAGGGA GCAATTCCAA TCGATCAGCC CCCTTTTGGA 
CCATCCACTC CAGGAAGCCA GGGCCCTGAC CTTCCCTTCC AGACACACAC TCAGTCCCAG 
GGTCATGGAC TCAACAAGTC CAGCTCCTCT CCTGAGCTCC AGACATTACC TGAAGCTTTT 
GCCAAGGCTG CTATGGAGTC AGAAAGTGCG ATGGTTGACA CGTCACGGCC TAGAGCACCC 
TCAGACGGCA TGCCTCAACC CCAGCAGCCC CAGTTTGCGA AAGAAAAGGT AGAGGGAAAC 
ACTGGAGTGG ACACTAATGT AGCGGGAGGT CCAAGCCCAG CAGGTGAAGG CCAAGCTGTA 
TCGTCATCTC AGAGTGGAGG AGCGGGGATG AGGTTGTCTT TTCCACCAGC ACCAGTACAA 
TCTGCACCCA TCTCCCCCAG TGGAGGCCAC CGGCCCAGAG GTCACACAAT CTCAGTCTCA 
GCCCCCTCCT CCAGGAGGGA GAGGCGGGCG GAGAGGGACG CTTACCACAG TCGACCTGGT 
CCCAGCAACA CTGAGAAAAT CTCTGGACTG AGCCCCAGCT TTGTGTTTCT GCAGCTCTAC 
CACTCTCCTT TCTTTGGGAA TGAAGCCAAC AAGCCACTTC TGCTGCCCAA AACTCAGGTG 
ATTGACCGTG CAGTAAAGGT TCTGGACCAA ATGCCCCCTT ATGACACCCA TAAAATTGGG 
GTGGTGTTTG TAGGAGCTGG TCAGGTTAAC AATGAAGTCG CCATCTTGTC GAACGAGTAC 
GGTTCGAACC GCTACGCCTC TTTCCTCACA GGTCTTGGCA AATTAATCCA TCTGAAAGAC 
TGCGATCCAG ACCAGATTTT TCTTGGAGGG CTTGACCAGT ATGGAGATGA TGGAGAGTTC 
ACCTACTGCT GGCATGACGA CATCATGCAG GCTATCTTTC ACATAGCCAC ACTGATGCCA 
AACAGGGAGA GCGACAAGGG TTGCTGCAAC AAAAAGCGAC ACATTGGCAA CGATTTTGTC 
ATGGTGGTGT ACAATGACTC TGGAGAGGAG TACAAACTGG GCACAATTAA GGGTCAGTTT 
AATTTTGTCG AAGTCATTAT AAAACCACTG GATTATGAAT GTAACCTCGT TACCCTCCAG 
TGCCGCAAAG ACCTCGAGGG ATTAGTAGAC ACAACGGTGG CAAAAATTGT TTCAGATCGA 
AACCTTCCCC TGTTAGTCAG GCAGATGGCT CTGCATGCAA ATATGGCCTC ATTGGTGCAC 
CAGTACAGAG CTAACCCCTC TGACGCATAC GCCTCTAAGT GGCTAGCAAG GTTACGACAC 
ATCAAGAGGA TCAGGACCAG GGCTCAAGAA GCCATCCAGT CTCGCTCAAC TCCCGGGATT 
TCACTGACCC AAGGGCAGAG CAAACCTTTG TCACAGAGCA GTGCTCCAAC AAATGTGGAA 
GCCACCGGAC AACGAAAAAG ACTGGTGTCC ACTGTAGACG ATTTCACAGA TTTTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tsc2 ( XM_004539036.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004539093.1 Maylandia zebra tuberous sclerosis 2 (tsc2), transcript variant X1, mRNA.

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CloneID	OMa18055
Clone ID Related Accession (Same CDS sequence)	XM_004539036.3 , XM_004539037.2 , XM_004539036.2 , XM_004539038.3 , XM_004539038.4
Accession Version	XM_004539036.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-10-15
Organism	Maylandia zebra(Zebra mbuna)
Product	tuberin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014446893.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_004539036.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAAAC AACCAAGCAA GGAGAGTCTC AAGGACAAGG TGAAGGGGAT CTTTGGGCTC 
GGTACACAGC GTCCTCCGAA CAAACAGAGT GACACTAAAC CCTCCGAGTT TATCATTACG 
GCTGACATCA TCAAGGAGCT CCACCCAGAT TGCGGTCTGA GCAACCGTGT TCGCACTATG 
AACCACATCT GTGAGCTTGC CAAAATGAAA AAGTTTGAGG AGCATGCAGT GGAGGCTGTA 
TGGAAAAATG TGGAAGACAT GCTGACTCCA GAGCAGCCAC CAGAGGCCAG ACATGCTGTC 
CTGCAGCTGC TCAGGGCCAT TATACAGGGG CAGGGTGAGC GACTCGGCCC TCTGAGAGCC 
TACTTTTTCA AGGTAATCAG AGACTACCAG CCGTGCAATG AAGACCTTTC TGACAGACTG 
GAGGTGTTTA AGGCTTTAAC AGAGAATGGA AAGGATATCA CATACCTGGA AGAGGACATA 
GCACGCTTTG TCCTCCTGTG GATGGATATA GGCCTTACAT CTGACTTTCT CCATGTTCTT 
GTTAATCTGG TGAAATTCAA TAGCTGCTAC TTGGACCAAA ATGTTTCCAT CATGGTCCAG 
AAAATTTGCC TGCTGTGCAA CAGAACGACG TCTTCGACTG ATATTGAGGT GGCTCTGCAA 
GTGCTCGATG CAGTTGTGTG TTACAACTGC CTGCCCTCCG ACTCTTTAAC CGTTTTCATA 
ATCACACTCT GCCGTACGGT GAACGTCAAA GAGTTTTGCG AGTCCTGTTG GAAGTTAATG 
AGAAAGGTTT TGGGGACTCA CCTCGGCCAT AGTGCAATTT ACACCATGTG TCGCATTATG 
GAGGAAAGGG TGTATATGGA GGATGCACCG TTGTTAAGAG GAGCAGTTTT CTTTGTTGGA 
ATGGCGCTTT GGGGCGCACA CAGACTGCCT GCACTCAAAA ATACACCGAC GCTTGTTCTG 
CCATCTTTCT ACAAGGCCAT GTCATGTGCC AACGAGGTGG TCTCCTACGA AATTGTTCTT 
TCAATAACAA GACTCATCAA AAAGTACGGT AAAGAGCTAC AAGTGGTCAC ATGGGACATC 
CTACTCGGCA TCATAGAGCG ACTGTTGCAA CAGATTCAGA CAATAGGCAG TGCGGAACTA 
AAGGCCATTG TTTATGAACT GTTGACCACC GTGGAAGAGC TTTATGAACA AAACGGTTAC 
CACGGCTCCA CTGAGAAGTT TTTCAGCCTG GTGGAGAAAT GTGCTGATAA AAGACCAGAT 
GCATCAGTGC TGACACTTAT CTCCTACAGG GCTCAGTCTA TCCAGCCTGC TAAAGAAGGC 
TGGATTCAGA GCCTTCACCG GCTCATGGAG AAATTCTTCA GGAACGAGAC TCGCAGTGTG 
ATAAGAATCA AAGTGCTTCA TATCCTGTCC TTCGTTCTCA GTACCAACCG GCAGCTCTAT 
GAGGATGAGT TGATTGAAAT GGTGGTGATT CCCCAGCTCA GTGGGATTGC TGAAGACCGG 
GACTTGGCAG TTAGAAAACA GGCTACTCAG CTATTAGTAG ACCTCGCTGA GGGCTGCAAC 
ACGCACCACT TCACCAGCCT GCTGGACATC ATTGAACGGG TGTCTAGCCG CTCTCTGGTG 
TGTTCAGGGC CCCTAGAGGT TTCTGAGAGA GACCCCACAG CAGAGTCTCC TATGGAGGAC 
GTCAGGACTG CTATTCTGGG CCTGCTTGAA ATCCTGCAGA GCAAACTTTA CAGTCTGCCA 
GCCAGCCATG CGAGTCGTGT TTATGAGTTG CTTATCAGCC ACCTGCAGCT TCATTACAAG 
AACAAGTACT GTTCAACTAT CGCCTCCACT ATTAGACTAA AGGTGTTTGA CTTCTTCCTC 
ATGATGCGTG CAGACTCTCT TCACCGTCTT GGGGTCCCAA ACAAAGATGG TGTCATGAGA 
TTCAGCCCTT ATTGCTACTG TGATATAGGG GAACCAGAGA AACGTGTTGG TGAAAAGAAG 
CCAGCAGGTT CAACCTCTCC TCCTGCTGGC AGCCCTGCTC CTCCTGCTGC TCCTCCCAGT 
TCAGCAGCCT CAACACGCTC AGCCTACCTT TCCTATTCGC CTGCCTTCAG TGTCCTCCTG 
CAGTGCCTCA AGATGGAGAC GGATTGGAAG GTGCTGAAGC TGGTTCTTGA CAAGCTGCCA 
TGGATGCTGC AGTACAAAGT GTTGCTGCTC ACATCACCAT GCAACTTAGA TCAGCTGTGT 
TCTACACTCT GCTGCATGGT GACAGATCGT CTCATCTCAG AGCGATTGAA AAAGACTCCA 
GAGGGGTTTT CTCGCACAGA TGTTCAGCTG GCAGTGATTC CCGTTCTTAC AGCAATTACC 
TCCTACCACA ACTACTTGGA GCAGTCAAGA CAGAGAGAAC TGGTTCAGTG CCTCGAGACT 
GGGCTCATTT ACCGCTGTGC CAAGCAATGT GTGGTGGCCC TTACAATGTG CACGGTGGAG 
ATGCCTGATA TCATGATCAA GCTTCTTCCT GCTCTTATCG TCAAGCTCAC TCACATCTCT 
GCTACTGTTG CCATGGCATC TCCTATGCTC GAGTTTCTCT CCACCCTCGT GCGCCTTCCC 
CATCTGTATG CCAACTTTGT AGCTGAGCAG TATGTCAGCG TGTTTGCCAT CTCACTACCT 
TACACTAACC CCTCAAAGTT CAACCAGTAC ATTGTGTCCC TGGCCCACCA TGTGATTGCC 
ATGTGGTTCA TACGCTGCAG ACTACCCTTC CGCAAGGACT TTGTTCAGTA TATCACAAAG 
GGTTTGCGTT CCAACGCCCT TCTGCCATTC GATGACAGTC ATGAGCAGAG TCCGTTTCGT 
GCCCGAAGCA CCAGCCTCAA TGAGAGACCC AAGAGCTTGC GGGCAGCAAA AGTGGCAAAG 
GCAGCAGCAG CGGTAGCCAA TAGCAGCAGC TCTCCAGTTA AAGAGCTGAG GGACCTTTCA 
GCCATGGACG CTTTCCGCTC CCGCAGCATC AGCGTCTCCG AACATGCGGT CCGCAGGATG 
CACACCTCAA CCACCACATG CAGTCTGGGC TCTGCCGATG AAAATGCAGT AACTCAGGCC 
GACGAGGGGC TGAAAACTGT CCACCTAGAG CTCACAGAGA CTTGTCTTGA CATGATGGCC 
CGATATGTAT TTTCCAACTT CTCTGCACTT CCCAAGAGGT CTCCCATCGC AGAGTTCCTG 
TTAGCAGGAG GCCGCAGTAT GACCTGGTTG GTGGGCAACA AGTTGGTCAC CATAACAACC 
AGCGGAGGGG TCAGAACACA AGCGCTGCTG GGCCTGGACA TGGCTGATCG CCTGGGAGGA 
GGAGGAGGGG AATTGACCAG GTCAGACCCA TCACTACACA CCCGAATGAC CAAAGAGGCT 
CCAGCCAAAT TGGAGTCACA GTCAAGCCAG CAGCACAACA GAGCTGCACG CATACGAGTT 
CGCTCCATGT CAGGTGGTCA TGCTCTACGT GCCGGTCCAG CTCAGAGTCT CAGTCCTCTG 
GTGTCTCCCT CTGAGGGTGA GTTGACTGCT CCACTCTCTC CCTCCTCTGG TCCTGCTCTG 
GATAGTTTAG GTCCATCCTC TTCCACCTCT GCCACTCCAT CTGTGCCTCC TACTCTGAAA 
GACAACCCCA CCTTGGCTGA ATTTGCCCCA ATGCTCACAC AAGGCTGGGC AGAGATCTTC 
ATACGCAGAC CGTCAGGTAA CACAAGCTGG CTAATGTGTT TGGAGAACCC ACCTAGCCCT 
TTCTCATCTG AGCTTGGCAA CATGCCGCTG CAAGAGCTCT CCAGTGTTCT GATGGCAATG 
GAGGGAGTGA AGGAACCTCC CACTCAGACA GCCAGTGCTC CTGCTAGCAC AGCTGTCCCT 
GCTCCAGCTC CTGTCTCTGA GCCCCTAACC CAAACACACA GCAGTGTTGG AGGAAAGGCA 
AACCTGATTC AGCGCTCCAA CACAGTGGGT GGCTCTCTCT GCTCTCTGGG TATGGGCACT 
GCCCCCGCAG GCCCACCAGC CCCTGGCAGG TTGCACAGGA GCATTTCCTG GGCAGACTCT 
GTCGTGGTGC TGGAGGAGGG ATCAGGAATT GCTACAGCAA ACACGCCGCC TGAATGGCTG 
GGGAGTGCAG AGTTTGAGCC AATGTTGTCT GATCCAATCT TCATAGCTGC TGATAAGTTT 
ACAAAGACTC CTCCTGAAAC ACTCAGCAGG TCCTCCTCCA CCTCAAGTCA GGATGATGAA 
AAGTCAACCC TGGAAGAAGT AAGTGAGGGA GCAATTCCAA TCGATCAGCC CCCTTTTGGA 
CCATCCACTC CAGGAAGCCA GGGCCCTGAC CTTCCCTTCC AGACACACAC TCAGTCCCAG 
GGTCATGGAC TCAACAAGTC CAGCTCCTCT CCTGAGCTCC AGACATTACC TGAAGCTTTT 
GCCAAGGCTG CTATGGAGTC AGAAAGTGCG ATGGTTGACA CGTCACGGCC TAGAGCACCC 
TCAGACGGCA TGCCTCAACC CCAGCAGCCC CAGTTTGCGA AAGAAAAGGT AGAGGGAAAC 
ACTGGAGTGG ACACTAATGT AGCGGGAGGT CCAAGCCCAG CAGGTGAAGG CCAAGCTGTA 
TCGTCATCTC AGAGTGGAGG AGCGGGGATG AGGTTGTCTT TTCCACCAGC ACCAGTACAA 
TCTGCACCCA TCTCCCCCAG TGGAGGCCAC CGGCCCAGAG GTCACACAAT CTCAGTCTCA 
GCCCCCTCCT CCAGGAGGGA GAGGCGGGCG GAGAGGGACG CTTACCACAG TCGACCTGGT 
CCCAGCAACA CTGAGAAAAT CTCTGGACTG AGCCCCAGCT TTGTGTTTCT GCAGCTCTAC 
CACTCTCCTT TCTTTGGGAA TGAAGCCAAC AAGCCACTTC TGCTGCCCAA AACTCAGGTG 
ATTGACCGTG CAGTAAAGGT TCTGGACCAA ATGCCCCCTT ATGACACCCA TAAAATTGGG 
GTGGTGTTTG TAGGAGCTGG TCAGGTTAAC AATGAAGTCG CCATCTTGTC GAACGAGTAC 
GGTTCGAACC GCTACGCCTC TTTCCTCACA GGTCTTGGCA AATTAATCCA TCTGAAAGAC 
TGCGATCCAG ACCAGATTTT TCTTGGAGGG CTTGACCAGT ATGGAGATGA TGGAGAGTTC 
ACCTACTGCT GGCATGACGA CATCATGCAG GCTATCTTTC ACATAGCCAC ACTGATGCCA 
AACAGGGAGA GCGACAAGGG TTGCTGCAAC AAAAAGCGAC ACATTGGCAA CGATTTTGTC 
ATGGTGGTGT ACAATGACTC TGGAGAGGAG TACAAACTGG GCACAATTAA GGGTCAGTTT 
AATTTTGTCG AAGTCATTAT AAAACCACTG GATTATGAAT GTAACCTCGT TACCCTCCAG 
TGCCGCAAAG ACCTCGAGGG ATTAGTAGAC ACAACGGTGG CAAAAATTGT TTCAGATCGA 
AACCTTCCCC TGTTAGTCAG GCAGATGGCT CTGCATGCAA ATATGGCCTC ATTGGTGCAC 
CAGTACAGAG CTAACCCCTC TGACGCATAC GCCTCTAAGT GGCTAGCAAG GTTACGACAC 
ATCAAGAGGA TCAGGACCAG GGCTCAAGAA GCCATCCAGT CTCGCTCAAC TCCCGGGATT 
TCACTGACCC AAGGGCAGAG CAAACCTTTG TCACAGAGCA GTGCTCCAAC AAATGTGGAA 
GCCACCGGAC AACGAAAAAG ACTGGTGTCC ACTGTAGACG ATTTCACAGA TTTTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004539093.1
CDS	196..5895
Translation	MNKQPSKESLKDKVKGIFGLGTQRPPNKQSDTKPSEFIITADIIKELHPDCGLSNRVRTMNHICELAKMKKFEEHAVEAVWKNVEDMLTPEQPPEARHAVLQLLRAIIQGQGERLGPLRAYFFKVIRDYQPCNEDLSDRLEVFKALTENGKDITYLEEDIARFVLLWMDIGLTSDFLHVLVNLVKFNSCYLDQNVSIMVQKICLLCNRTTSSTDIEVALQVLDAVVCYNCLPSDSLTVFIITLCRTVNVKEFCESCWKLMRKVLGTHLGHSAIYTMCRIMEERVYMEDAPLLRGAVFFVGMALWGAHRLPALKNTPTLVLPSFYKAMSCANEVVSYEIVLSITRLIKKYGKELQVVTWDILLGIIERLLQQIQTIGSAELKAIVYELLTTVEELYEQNGYHGSTEKFFSLVEKCADKRPDASVLTLISYRAQSIQPAKEGWIQSLHRLMEKFFRNETRSVIRIKVLHILSFVLSTNRQLYEDELIEMVVIPQLSGIAEDRDLAVRKQATQLLVDLAEGCNTHHFTSLLDIIERVSSRSLVCSGPLEVSERDPTAESPMEDVRTAILGLLEILQSKLYSLPASHASRVYELLISHLQLHYKNKYCSTIASTIRLKVFDFFLMMRADSLHRLGVPNKDGVMRFSPYCYCDIGEPEKRVGEKKPAGSTSPPAGSPAPPAAPPSSAASTRSAYLSYSPAFSVLLQCLKMETDWKVLKLVLDKLPWMLQYKVLLLTSPCNLDQLCSTLCCMVTDRLISERLKKTPEGFSRTDVQLAVIPVLTAITSYHNYLEQSRQRELVQCLETGLIYRCAKQCVVALTMCTVEMPDIMIKLLPALIVKLTHISATVAMASPMLEFLSTLVRLPHLYANFVAEQYVSVFAISLPYTNPSKFNQYIVSLAHHVIAMWFIRCRLPFRKDFVQYITKGLRSNALLPFDDSHEQSPFRARSTSLNERPKSLRAAKVAKAAAAVANSSSSPVKELRDLSAMDAFRSRSISVSEHAVRRMHTSTTTCSLGSADENAVTQADEGLKTVHLELTETCLDMMARYVFSNFSALPKRSPIAEFLLAGGRSMTWLVGNKLVTITTSGGVRTQALLGLDMADRLGGGGGELTRSDPSLHTRMTKEAPAKLESQSSQQHNRAARIRVRSMSGGHALRAGPAQSLSPLVSPSEGELTAPLSPSSGPALDSLGPSSSTSATPSVPPTLKDNPTLAEFAPMLTQGWAEIFIRRPSGNTSWLMCLENPPSPFSSELGNMPLQELSSVLMAMEGVKEPPTQTASAPASTAVPAPAPVSEPLTQTHSSVGGKANLIQRSNTVGGSLCSLGMGTAPAGPPAPGRLHRSISWADSVVVLEEGSGIATANTPPEWLGSAEFEPMLSDPIFIAADKFTKTPPETLSRSSSTSSQDDEKSTLEEVSEGAIPIDQPPFGPSTPGSQGPDLPFQTHTQSQGHGLNKSSSSPELQTLPEAFAKAAMESESAMVDTSRPRAPSDGMPQPQQPQFAKEKVEGNTGVDTNVAGGPSPAGEGQAVSSSQSGGAGMRLSFPPAPVQSAPISPSGGHRPRGHTISVSAPSSRRERRAERDAYHSRPGPSNTEKISGLSPSFVFLQLYHSPFFGNEANKPLLLPKTQVIDRAVKVLDQMPPYDTHKIGVVFVGAGQVNNEVAILSNEYGSNRYASFLTGLGKLIHLKDCDPDQIFLGGLDQYGDDGEFTYCWHDDIMQAIFHIATLMPNRESDKGCCNKKRHIGNDFVMVVYNDSGEEYKLGTIKGQFNFVEVIIKPLDYECNLVTLQCRKDLEGLVDTTVAKIVSDRNLPLLVRQMALHANMASLVHQYRANPSDAYASKWLARLRHIKRIRTRAQEAIQSRSTPGISLTQGQSKPLSQSSAPTNVEATGQRKRLVSTVDDFTDFV

Target ORF information:

RefSeq Version	XM_004539036.2
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra tuberous sclerosis 2 (tsc2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004539036.2

ORF Insert Sequence:

ATGAATAAAC AACCAAGCAA GGAGAGTCTC AAGGACAAGG TGAAGGGGAT CTTTGGGCTC 
GGTACACAGC GTCCTCCGAA CAAACAGAGT GACACTAAAC CCTCCGAGTT TATCATTACG 
GCTGACATCA TCAAGGAGCT CCACCCAGAT TGCGGTCTGA GCAACCGTGT TCGCACTATG 
AACCACATCT GTGAGCTTGC CAAAATGAAA AAGTTTGAGG AGCATGCAGT GGAGGCTGTA 
TGGAAAAATG TGGAAGACAT GCTGACTCCA GAGCAGCCAC CAGAGGCCAG ACATGCTGTC 
CTGCAGCTGC TCAGGGCCAT TATACAGGGG CAGGGTGAGC GACTCGGCCC TCTGAGAGCC 
TACTTTTTCA AGGTAATCAG AGACTACCAG CCGTGCAATG AAGACCTTTC TGACAGACTG 
GAGGTGTTTA AGGCTTTAAC AGAGAATGGA AAGGATATCA CATACCTGGA AGAGGACATA 
GCACGCTTTG TCCTCCTGTG GATGGATATA GGCCTTACAT CTGACTTTCT CCATGTTCTT 
GTTAATCTGG TGAAATTCAA TAGCTGCTAC TTGGACCAAA ATGTTTCCAT CATGGTCCAG 
AAAATTTGCC TGCTGTGCAA CAGAACGACG TCTTCGACTG ATATTGAGGT GGCTCTGCAA 
GTGCTCGATG CAGTTGTGTG TTACAACTGC CTGCCCTCCG ACTCTTTAAC CGTTTTCATA 
ATCACACTCT GCCGTACGGT GAACGTCAAA GAGTTTTGCG AGTCCTGTTG GAAGTTAATG 
AGAAAGGTTT TGGGGACTCA CCTCGGCCAT AGTGCAATTT ACACCATGTG TCGCATTATG 
GAGGAAAGGG TGTATATGGA GGATGCACCG TTGTTAAGAG GAGCAGTTTT CTTTGTTGGA 
ATGGCGCTTT GGGGCGCACA CAGACTGCCT GCACTCAAAA ATACACCGAC GCTTGTTCTG 
CCATCTTTCT ACAAGGCCAT GTCATGTGCC AACGAGGTGG TCTCCTACGA AATTGTTCTT 
TCAATAACAA GACTCATCAA AAAGTACGGT AAAGAGCTAC AAGTGGTCAC ATGGGACATC 
CTACTCGGCA TCATAGAGCG ACTGTTGCAA CAGATTCAGA CAATAGGCAG TGCGGAACTA 
AAGGCCATTG TTTATGAACT GTTGACCACC GTGGAAGAGC TTTATGAACA AAACGGTTAC 
CACGGCTCCA CTGAGAAGTT TTTCAGCCTG GTGGAGAAAT GTGCTGATAA AAGACCAGAT 
GCATCAGTGC TGACACTTAT CTCCTACAGG GCTCAGTCTA TCCAGCCTGC TAAAGAAGGC 
TGGATTCAGA GCCTTCACCG GCTCATGGAG AAATTCTTCA GGAACGAGAC TCGCAGTGTG 
ATAAGAATCA AAGTGCTTCA TATCCTGTCC TTCGTTCTCA GTACCAACCG GCAGCTCTAT 
GAGGATGAGT TGATTGAAAT GGTGGTGATT CCCCAGCTCA GTGGGATTGC TGAAGACCGG 
GACTTGGCAG TTAGAAAACA GGCTACTCAG CTATTAGTAG ACCTCGCTGA GGGCTGCAAC 
ACGCACCACT TCACCAGCCT GCTGGACATC ATTGAACGGG TGTCTAGCCG CTCTCTGGTG 
TGTTCAGGGC CCCTAGAGGT TTCTGAGAGA GACCCCACAG CAGAGTCTCC TATGGAGGAC 
GTCAGGACTG CTATTCTGGG CCTGCTTGAA ATCCTGCAGA GCAAACTTTA CAGTCTGCCA 
GCCAGCCATG CGAGTCGTGT TTATGAGTTG CTTATCAGCC ACCTGCAGCT TCATTACAAG 
AACAAGTACT GTTCAACTAT CGCCTCCACT ATTAGACTAA AGGTGTTTGA CTTCTTCCTC 
ATGATGCGTG CAGACTCTCT TCACCGTCTT GGGGTCCCAA ACAAAGATGG TGTCATGAGA 
TTCAGCCCTT ATTGCTACTG TGATATAGGG GAACCAGAGA AACGTGTTGG TGAAAAGAAG 
CCAGCAGGTT CAACCTCTCC TCCTGCTGGC AGCCCTGCTC CTCCTGCTGC TCCTCCCAGT 
TCAGCAGCCT CAACACGCTC AGCCTACCTT TCCTATTCGC CTGCCTTCAG TGTCCTCCTG 
CAGTGCCTCA AGATGGAGAC GGATTGGAAG GTGCTGAAGC TGGTTCTTGA CAAGCTGCCA 
TGGATGCTGC AGTACAAAGT GTTGCTGCTC ACATCACCAT GCAACTTAGA TCAGCTGTGT 
TCTACACTCT GCTGCATGGT GACAGATCGT CTCATCTCAG AGCGATTGAA AAAGACTCCA 
GAGGGGTTTT CTCGCACAGA TGTTCAGCTG GCAGTGATTC CCGTTCTTAC AGCAATTACC 
TCCTACCACA ACTACTTGGA GCAGTCAAGA CAGAGAGAAC TGGTTCAGTG CCTCGAGACT 
GGGCTCATTT ACCGCTGTGC CAAGCAATGT GTGGTGGCCC TTACAATGTG CACGGTGGAG 
ATGCCTGATA TCATGATCAA GCTTCTTCCT GCTCTTATCG TCAAGCTCAC TCACATCTCT 
GCTACTGTTG CCATGGCATC TCCTATGCTC GAGTTTCTCT CCACCCTCGT GCGCCTTCCC 
CATCTGTATG CCAACTTTGT AGCTGAGCAG TATGTCAGCG TGTTTGCCAT CTCACTACCT 
TACACTAACC CCTCAAAGTT CAACCAGTAC ATTGTGTCCC TGGCCCACCA TGTGATTGCC 
ATGTGGTTCA TACGCTGCAG ACTACCCTTC CGCAAGGACT TTGTTCAGTA TATCACAAAG 
GGTTTGCGTT CCAACGCCCT TCTGCCATTC GATGACAGTC ATGAGCAGAG TCCGTTTCGT 
GCCCGAAGCA CCAGCCTCAA TGAGAGACCC AAGAGCTTGC GGGCAGCAAA AGTGGCAAAG 
GCAGCAGCAG CGGTAGCCAA TAGCAGCAGC TCTCCAGTTA AAGAGCTGAG GGACCTTTCA 
GCCATGGACG CTTTCCGCTC CCGCAGCATC AGCGTCTCCG AACATGCGGT CCGCAGGATG 
CACACCTCAA CCACCACATG CAGTCTGGGC TCTGCCGATG AAAATGCAGT AACTCAGGCC 
GACGAGGGGC TGAAAACTGT CCACCTAGAG CTCACAGAGA CTTGTCTTGA CATGATGGCC 
CGATATGTAT TTTCCAACTT CTCTGCACTT CCCAAGAGGT CTCCCATCGC AGAGTTCCTG 
TTAGCAGGAG GCCGCAGTAT GACCTGGTTG GTGGGCAACA AGTTGGTCAC CATAACAACC 
AGCGGAGGGG TCAGAACACA AGCGCTGCTG GGCCTGGACA TGGCTGATCG CCTGGGAGGA 
GGAGGAGGGG AATTGACCAG GTCAGACCCA TCACTACACA CCCGAATGAC CAAAGAGGCT 
CCAGCCAAAT TGGAGTCACA GTCAAGCCAG CAGCACAACA GAGCTGCACG CATACGAGTT 
CGCTCCATGT CAGGTGGTCA TGCTCTACGT GCCGGTCCAG CTCAGAGTCT CAGTCCTCTG 
GTGTCTCCCT CTGAGGGTGA GTTGACTGCT CCACTCTCTC CCTCCTCTGG TCCTGCTCTG 
GATAGTTTAG GTCCATCCTC TTCCACCTCT GCCACTCCAT CTGTGCCTCC TACTCTGAAA 
GACAACCCCA CCTTGGCTGA ATTTGCCCCA ATGCTCACAC AAGGCTGGGC AGAGATCTTC 
ATACGCAGAC CGTCAGGTAA CACAAGCTGG CTAATGTGTT TGGAGAACCC ACCTAGCCCT 
TTCTCATCTG AGCTTGGCAA CATGCCGCTG CAAGAGCTCT CCAGTGTTCT GATGGCAATG 
GAGGGAGTGA AGGAACCTCC CACTCAGACA GCCAGTGCTC CTGCTAGCAC AGCTGTCCCT 
GCTCCAGCTC CTGTCTCTGA GCCCCTAACC CAAACACACA GCAGTGTTGG AGGAAAGGCA 
AACCTGATTC AGCGCTCCAA CACAGTGGGT GGCTCTCTCT GCTCTCTGGG TATGGGCACT 
GCCCCCGCAG GCCCACCAGC CCCTGGCAGG TTGCACAGGA GCATTTCCTG GGCAGACTCT 
GTCGTGGTGC TGGAGGAGGG ATCAGGAATT GCTACAGCAA ACACGCCGCC TGAATGGCTG 
GGGAGTGCAG AGTTTGAGCC AATGTTGTCT GATCCAATCT TCATAGCTGC TGATAAGTTT 
ACAAAGACTC CTCCTGAAAC ACTCAGCAGG TCCTCCTCCA CCTCAAGTCA GGATGATGAA 
AAGTCAACCC TGGAAGAAGT AAGTGAGGGA GCAATTCCAA TCGATCAGCC CCCTTTTGGA 
CCATCCACTC CAGGAAGCCA GGGCCCTGAC CTTCCCTTCC AGACACACAC TCAGTCCCAG 
GGTCATGGAC TCAACAAGTC CAGCTCCTCT CCTGAGCTCC AGACATTACC TGAAGCTTTT 
GCCAAGGCTG CTATGGAGTC AGAAAGTGCG ATGGTTGACA CGTCACGGCC TAGAGCACCC 
TCAGACGGCA TGCCTCAACC CCAGCAGCCC CAGTTTGCGA AAGAAAAGGT AGAGGGAAAC 
ACTGGAGTGG ACACTAATGT AGCGGGAGGT CCAAGCCCAG CAGGTGAAGG CCAAGCTGTA 
TCGTCATCTC AGAGTGGAGG AGCGGGGATG AGGTTGTCTT TTCCACCAGC ACCAGTACAA 
TCTGCACCCA TCTCCCCCAG TGGAGGCCAC CGGCCCAGAG GTCACACAAT CTCAGTCTCA 
GCCCCCTCCT CCAGGAGGGA GAGGCGGGCG GAGAGGGACG CTTACCACAG TCGACCTGGT 
CCCAGCAACA CTGAGAAAAT CTCTGGACTG AGCCCCAGCT TTGTGTTTCT GCAGCTCTAC 
CACTCTCCTT TCTTTGGGAA TGAAGCCAAC AAGCCACTTC TGCTGCCCAA AACTCAGGTG 
ATTGACCGTG CAGTAAAGGT TCTGGACCAA ATGCCCCCTT ATGACACCCA TAAAATTGGG 
GTGGTGTTTG TAGGAGCTGG TCAGGTTAAC AATGAAGTCG CCATCTTGTC GAACGAGTAC 
GGTTCGAACC GCTACGCCTC TTTCCTCACA GGTCTTGGCA AATTAATCCA TCTGAAAGAC 
TGCGATCCAG ACCAGATTTT TCTTGGAGGG CTTGACCAGT ATGGAGATGA TGGAGAGTTC 
ACCTACTGCT GGCATGACGA CATCATGCAG GCTATCTTTC ACATAGCCAC ACTGATGCCA 
AACAGGGAGA GCGACAAGGG TTGCTGCAAC AAAAAGCGAC ACATTGGCAA CGATTTTGTC 
ATGGTGGTGT ACAATGACTC TGGAGAGGAG TACAAACTGG GCACAATTAA GGGTCAGTTT 
AATTTTGTCG AAGTCATTAT AAAACCACTG GATTATGAAT GTAACCTCGT TACCCTCCAG 
TGCCGCAAAG ACCTCGAGGG ATTAGTAGAC ACAACGGTGG CAAAAATTGT TTCAGATCGA 
AACCTTCCCC TGTTAGTCAG GCAGATGGCT CTGCATGCAA ATATGGCCTC ATTGGTGCAC 
CAGTACAGAG CTAACCCCTC TGACGCATAC GCCTCTAAGT GGCTAGCAAG GTTACGACAC 
ATCAAGAGGA TCAGGACCAG GGCTCAAGAA GCCATCCAGT CTCGCTCAAC TCCCGGGATT 
TCACTGACCC AAGGGCAGAG CAAACCTTTG TCACAGAGCA GTGCTCCAAC AAATGTGGAA 
GCCACCGGAC AACGAAAAAG ACTGGTGTCC ACTGTAGACG ATTTCACAGA TTTTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tsc2 ( XM_004539038.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004539095.1 Maylandia zebra tuberous sclerosis 2 (tsc2), transcript variant X3, mRNA.

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CloneID	OMa18055
Clone ID Related Accession (Same CDS sequence)	XM_004539036.3 , XM_004539037.2 , XM_004539036.2 , XM_004539038.3 , XM_004539038.4
Accession Version	XM_004539038.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-10-15
Organism	Maylandia zebra(Zebra mbuna)
Product	tuberin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014446893.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_004539038.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAAAC AACCAAGCAA GGAGAGTCTC AAGGACAAGG TGAAGGGGAT CTTTGGGCTC 
GGTACACAGC GTCCTCCGAA CAAACAGAGT GACACTAAAC CCTCCGAGTT TATCATTACG 
GCTGACATCA TCAAGGAGCT CCACCCAGAT TGCGGTCTGA GCAACCGTGT TCGCACTATG 
AACCACATCT GTGAGCTTGC CAAAATGAAA AAGTTTGAGG AGCATGCAGT GGAGGCTGTA 
TGGAAAAATG TGGAAGACAT GCTGACTCCA GAGCAGCCAC CAGAGGCCAG ACATGCTGTC 
CTGCAGCTGC TCAGGGCCAT TATACAGGGG CAGGGTGAGC GACTCGGCCC TCTGAGAGCC 
TACTTTTTCA AGGTAATCAG AGACTACCAG CCGTGCAATG AAGACCTTTC TGACAGACTG 
GAGGTGTTTA AGGCTTTAAC AGAGAATGGA AAGGATATCA CATACCTGGA AGAGGACATA 
GCACGCTTTG TCCTCCTGTG GATGGATATA GGCCTTACAT CTGACTTTCT CCATGTTCTT 
GTTAATCTGG TGAAATTCAA TAGCTGCTAC TTGGACCAAA ATGTTTCCAT CATGGTCCAG 
AAAATTTGCC TGCTGTGCAA CAGAACGACG TCTTCGACTG ATATTGAGGT GGCTCTGCAA 
GTGCTCGATG CAGTTGTGTG TTACAACTGC CTGCCCTCCG ACTCTTTAAC CGTTTTCATA 
ATCACACTCT GCCGTACGGT GAACGTCAAA GAGTTTTGCG AGTCCTGTTG GAAGTTAATG 
AGAAAGGTTT TGGGGACTCA CCTCGGCCAT AGTGCAATTT ACACCATGTG TCGCATTATG 
GAGGAAAGGG TGTATATGGA GGATGCACCG TTGTTAAGAG GAGCAGTTTT CTTTGTTGGA 
ATGGCGCTTT GGGGCGCACA CAGACTGCCT GCACTCAAAA ATACACCGAC GCTTGTTCTG 
CCATCTTTCT ACAAGGCCAT GTCATGTGCC AACGAGGTGG TCTCCTACGA AATTGTTCTT 
TCAATAACAA GACTCATCAA AAAGTACGGT AAAGAGCTAC AAGTGGTCAC ATGGGACATC 
CTACTCGGCA TCATAGAGCG ACTGTTGCAA CAGATTCAGA CAATAGGCAG TGCGGAACTA 
AAGGCCATTG TTTATGAACT GTTGACCACC GTGGAAGAGC TTTATGAACA AAACGGTTAC 
CACGGCTCCA CTGAGAAGTT TTTCAGCCTG GTGGAGAAAT GTGCTGATAA AAGACCAGAT 
GCATCAGTGC TGACACTTAT CTCCTACAGG GCTCAGTCTA TCCAGCCTGC TAAAGAAGGC 
TGGATTCAGA GCCTTCACCG GCTCATGGAG AAATTCTTCA GGAACGAGAC TCGCAGTGTG 
ATAAGAATCA AAGTGCTTCA TATCCTGTCC TTCGTTCTCA GTACCAACCG GCAGCTCTAT 
GAGGATGAGT TGATTGAAAT GGTGGTGATT CCCCAGCTCA GTGGGATTGC TGAAGACCGG 
GACTTGGCAG TTAGAAAACA GGCTACTCAG CTATTAGTAG ACCTCGCTGA GGGCTGCAAC 
ACGCACCACT TCACCAGCCT GCTGGACATC ATTGAACGGG TGTCTAGCCG CTCTCTGGTG 
TGTTCAGGGC CCCTAGAGGT TTCTGAGAGA GACCCCACAG CAGAGTCTCC TATGGAGGAC 
GTCAGGACTG CTATTCTGGG CCTGCTTGAA ATCCTGCAGA GCAAACTTTA CAGTCTGCCA 
GCCAGCCATG CGAGTCGTGT TTATGAGTTG CTTATCAGCC ACCTGCAGCT TCATTACAAG 
AACAAGTACT GTTCAACTAT CGCCTCCACT ATTAGACTAA AGGTGTTTGA CTTCTTCCTC 
ATGATGCGTG CAGACTCTCT TCACCGTCTT GGGGTCCCAA ACAAAGATGG TGTCATGAGA 
TTCAGCCCTT ATTGCTACTG TGATATAGGG GAACCAGAGA AACGTGTTGG TGAAAAGAAG 
CCAGCAGGTT CAACCTCTCC TCCTGCTGGC AGCCCTGCTC CTCCTGCTGC TCCTCCCAGT 
TCAGCAGCCT CAACACGCTC AGCCTACCTT TCCTATTCGC CTGCCTTCAG TGTCCTCCTG 
CAGTGCCTCA AGATGGAGAC GGATTGGAAG GTGCTGAAGC TGGTTCTTGA CAAGCTGCCA 
TGGATGCTGC AGTACAAAGT GTTGCTGCTC ACATCACCAT GCAACTTAGA TCAGCTGTGT 
TCTACACTCT GCTGCATGGT GACAGATCGT CTCATCTCAG AGCGATTGAA AAAGACTCCA 
GAGGGGTTTT CTCGCACAGA TGTTCAGCTG GCAGTGATTC CCGTTCTTAC AGCAATTACC 
TCCTACCACA ACTACTTGGA GCAGTCAAGA CAGAGAGAAC TGGTTCAGTG CCTCGAGACT 
GGGCTCATTT ACCGCTGTGC CAAGCAATGT GTGGTGGCCC TTACAATGTG CACGGTGGAG 
ATGCCTGATA TCATGATCAA GCTTCTTCCT GCTCTTATCG TCAAGCTCAC TCACATCTCT 
GCTACTGTTG CCATGGCATC TCCTATGCTC GAGTTTCTCT CCACCCTCGT GCGCCTTCCC 
CATCTGTATG CCAACTTTGT AGCTGAGCAG TATGTCAGCG TGTTTGCCAT CTCACTACCT 
TACACTAACC CCTCAAAGTT CAACCAGTAC ATTGTGTCCC TGGCCCACCA TGTGATTGCC 
ATGTGGTTCA TACGCTGCAG ACTACCCTTC CGCAAGGACT TTGTTCAGTA TATCACAAAG 
GGTTTGCGTT CCAACGCCCT TCTGCCATTC GATGACAGTC ATGAGCAGAG TCCGTTTCGT 
GCCCGAAGCA CCAGCCTCAA TGAGAGACCC AAGAGCTTGC GGGCAGCAAA AGTGGCAAAG 
GCAGCAGCAG CGGTAGCCAA TAGCAGCAGC TCTCCAGTTA AAGAGCTGAG GGACCTTTCA 
GCCATGGACG CTTTCCGCTC CCGCAGCATC AGCGTCTCCG AACATGCGGT CCGCAGGATG 
CACACCTCAA CCACCACATG CAGTCTGGGC TCTGCCGATG AAAATGCAGT AACTCAGGCC 
GACGAGGGGC TGAAAACTGT CCACCTAGAG CTCACAGAGA CTTGTCTTGA CATGATGGCC 
CGATATGTAT TTTCCAACTT CTCTGCACTT CCCAAGAGGT CTCCCATCGC AGAGTTCCTG 
TTAGCAGGAG GCCGCAGTAT GACCTGGTTG GTGGGCAACA AGTTGGTCAC CATAACAACC 
AGCGGAGGGG TCAGAACACA AGCGCTGCTG GGCCTGGACA TGGCTGATCG CCTGGGAGGA 
GGAGGAGGGG AATTGACCAG GTCAGACCCA TCACTACACA CCCGAATGAC CAAAGAGGCT 
CCAGCCAAAT TGGAGTCACA GTCAAGCCAG CAGCACAACA GAGCTGCACG CATACGAGTT 
CGCTCCATGT CAGGTGGTCA TGCTCTACGT GCCGGTCCAG CTCAGAGTCT CAGTCCTCTG 
GTGTCTCCCT CTGAGGGTGA GTTGACTGCT CCACTCTCTC CCTCCTCTGG TCCTGCTCTG 
GATAGTTTAG GTCCATCCTC TTCCACCTCT GCCACTCCAT CTGTGCCTCC TACTCTGAAA 
GACAACCCCA CCTTGGCTGA ATTTGCCCCA ATGCTCACAC AAGGCTGGGC AGAGATCTTC 
ATACGCAGAC CGTCAGGTAA CACAAGCTGG CTAATGTGTT TGGAGAACCC ACCTAGCCCT 
TTCTCATCTG AGCTTGGCAA CATGCCGCTG CAAGAGCTCT CCAGTGTTCT GATGGCAATG 
GAGGGAGTGA AGGAACCTCC CACTCAGACA GCCAGTGCTC CTGCTAGCAC AGCTGTCCCT 
GCTCCAGCTC CTGTCTCTGA GCCCCTAACC CAAACACACA GCAGTGTTGG AGGAAAGGCA 
AACCTGATTC AGCGCTCCAA CACAGTGGGT GGCTCTCTCT GCTCTCTGGG TATGGGCACT 
GCCCCCGCAG GCCCACCAGC CCCTGGCAGG TTGCACAGGA GCATTTCCTG GGCAGACTCT 
GTCGTGGTGC TGGAGGAGGG ATCAGGAATT GCTACAGCAA ACACGCCGCC TGAATGGCTG 
GGGAGTGCAG AGTTTGAGCC AATGTTGTCT GATCCAATCT TCATAGCTGC TGATAAGTTT 
ACAAAGACTC CTCCTGAAAC ACTCAGCAGG TCCTCCTCCA CCTCAAGTCA GGATGATGAA 
AAGTCAACCC TGGAAGAAGT AAGTGAGGGA GCAATTCCAA TCGATCAGCC CCCTTTTGGA 
CCATCCACTC CAGGAAGCCA GGGCCCTGAC CTTCCCTTCC AGACACACAC TCAGTCCCAG 
GGTCATGGAC TCAACAAGTC CAGCTCCTCT CCTGAGCTCC AGACATTACC TGAAGCTTTT 
GCCAAGGCTG CTATGGAGTC AGAAAGTGCG ATGGTTGACA CGTCACGGCC TAGAGCACCC 
TCAGACGGCA TGCCTCAACC CCAGCAGCCC CAGTTTGCGA AAGAAAAGGT AGAGGGAAAC 
ACTGGAGTGG ACACTAATGT AGCGGGAGGT CCAAGCCCAG CAGGTGAAGG CCAAGCTGTA 
TCGTCATCTC AGAGTGGAGG AGCGGGGATG AGGTTGTCTT TTCCACCAGC ACCAGTACAA 
TCTGCACCCA TCTCCCCCAG TGGAGGCCAC CGGCCCAGAG GTCACACAAT CTCAGTCTCA 
GCCCCCTCCT CCAGGAGGGA GAGGCGGGCG GAGAGGGACG CTTACCACAG TCGACCTGGT 
CCCAGCAACA CTGAGAAAAT CTCTGGACTG AGCCCCAGCT TTGTGTTTCT GCAGCTCTAC 
CACTCTCCTT TCTTTGGGAA TGAAGCCAAC AAGCCACTTC TGCTGCCCAA AACTCAGGTG 
ATTGACCGTG CAGTAAAGGT TCTGGACCAA ATGCCCCCTT ATGACACCCA TAAAATTGGG 
GTGGTGTTTG TAGGAGCTGG TCAGGTTAAC AATGAAGTCG CCATCTTGTC GAACGAGTAC 
GGTTCGAACC GCTACGCCTC TTTCCTCACA GGTCTTGGCA AATTAATCCA TCTGAAAGAC 
TGCGATCCAG ACCAGATTTT TCTTGGAGGG CTTGACCAGT ATGGAGATGA TGGAGAGTTC 
ACCTACTGCT GGCATGACGA CATCATGCAG GCTATCTTTC ACATAGCCAC ACTGATGCCA 
AACAGGGAGA GCGACAAGGG TTGCTGCAAC AAAAAGCGAC ACATTGGCAA CGATTTTGTC 
ATGGTGGTGT ACAATGACTC TGGAGAGGAG TACAAACTGG GCACAATTAA GGGTCAGTTT 
AATTTTGTCG AAGTCATTAT AAAACCACTG GATTATGAAT GTAACCTCGT TACCCTCCAG 
TGCCGCAAAG ACCTCGAGGG ATTAGTAGAC ACAACGGTGG CAAAAATTGT TTCAGATCGA 
AACCTTCCCC TGTTAGTCAG GCAGATGGCT CTGCATGCAA ATATGGCCTC ATTGGTGCAC 
CAGTACAGAG CTAACCCCTC TGACGCATAC GCCTCTAAGT GGCTAGCAAG GTTACGACAC 
ATCAAGAGGA TCAGGACCAG GGCTCAAGAA GCCATCCAGT CTCGCTCAAC TCCCGGGATT 
TCACTGACCC AAGGGCAGAG CAAACCTTTG TCACAGAGCA GTGCTCCAAC AAATGTGGAA 
GCCACCGGAC AACGAAAAAG ACTGGTGTCC ACTGTAGACG ATTTCACAGA TTTTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004539095.1
CDS	73..5772
Translation	MNKQPSKESLKDKVKGIFGLGTQRPPNKQSDTKPSEFIITADIIKELHPDCGLSNRVRTMNHICELAKMKKFEEHAVEAVWKNVEDMLTPEQPPEARHAVLQLLRAIIQGQGERLGPLRAYFFKVIRDYQPCNEDLSDRLEVFKALTENGKDITYLEEDIARFVLLWMDIGLTSDFLHVLVNLVKFNSCYLDQNVSIMVQKICLLCNRTTSSTDIEVALQVLDAVVCYNCLPSDSLTVFIITLCRTVNVKEFCESCWKLMRKVLGTHLGHSAIYTMCRIMEERVYMEDAPLLRGAVFFVGMALWGAHRLPALKNTPTLVLPSFYKAMSCANEVVSYEIVLSITRLIKKYGKELQVVTWDILLGIIERLLQQIQTIGSAELKAIVYELLTTVEELYEQNGYHGSTEKFFSLVEKCADKRPDASVLTLISYRAQSIQPAKEGWIQSLHRLMEKFFRNETRSVIRIKVLHILSFVLSTNRQLYEDELIEMVVIPQLSGIAEDRDLAVRKQATQLLVDLAEGCNTHHFTSLLDIIERVSSRSLVCSGPLEVSERDPTAESPMEDVRTAILGLLEILQSKLYSLPASHASRVYELLISHLQLHYKNKYCSTIASTIRLKVFDFFLMMRADSLHRLGVPNKDGVMRFSPYCYCDIGEPEKRVGEKKPAGSTSPPAGSPAPPAAPPSSAASTRSAYLSYSPAFSVLLQCLKMETDWKVLKLVLDKLPWMLQYKVLLLTSPCNLDQLCSTLCCMVTDRLISERLKKTPEGFSRTDVQLAVIPVLTAITSYHNYLEQSRQRELVQCLETGLIYRCAKQCVVALTMCTVEMPDIMIKLLPALIVKLTHISATVAMASPMLEFLSTLVRLPHLYANFVAEQYVSVFAISLPYTNPSKFNQYIVSLAHHVIAMWFIRCRLPFRKDFVQYITKGLRSNALLPFDDSHEQSPFRARSTSLNERPKSLRAAKVAKAAAAVANSSSSPVKELRDLSAMDAFRSRSISVSEHAVRRMHTSTTTCSLGSADENAVTQADEGLKTVHLELTETCLDMMARYVFSNFSALPKRSPIAEFLLAGGRSMTWLVGNKLVTITTSGGVRTQALLGLDMADRLGGGGGELTRSDPSLHTRMTKEAPAKLESQSSQQHNRAARIRVRSMSGGHALRAGPAQSLSPLVSPSEGELTAPLSPSSGPALDSLGPSSSTSATPSVPPTLKDNPTLAEFAPMLTQGWAEIFIRRPSGNTSWLMCLENPPSPFSSELGNMPLQELSSVLMAMEGVKEPPTQTASAPASTAVPAPAPVSEPLTQTHSSVGGKANLIQRSNTVGGSLCSLGMGTAPAGPPAPGRLHRSISWADSVVVLEEGSGIATANTPPEWLGSAEFEPMLSDPIFIAADKFTKTPPETLSRSSSTSSQDDEKSTLEEVSEGAIPIDQPPFGPSTPGSQGPDLPFQTHTQSQGHGLNKSSSSPELQTLPEAFAKAAMESESAMVDTSRPRAPSDGMPQPQQPQFAKEKVEGNTGVDTNVAGGPSPAGEGQAVSSSQSGGAGMRLSFPPAPVQSAPISPSGGHRPRGHTISVSAPSSRRERRAERDAYHSRPGPSNTEKISGLSPSFVFLQLYHSPFFGNEANKPLLLPKTQVIDRAVKVLDQMPPYDTHKIGVVFVGAGQVNNEVAILSNEYGSNRYASFLTGLGKLIHLKDCDPDQIFLGGLDQYGDDGEFTYCWHDDIMQAIFHIATLMPNRESDKGCCNKKRHIGNDFVMVVYNDSGEEYKLGTIKGQFNFVEVIIKPLDYECNLVTLQCRKDLEGLVDTTVAKIVSDRNLPLLVRQMALHANMASLVHQYRANPSDAYASKWLARLRHIKRIRTRAQEAIQSRSTPGISLTQGQSKPLSQSSAPTNVEATGQRKRLVSTVDDFTDFV

Target ORF information:

RefSeq Version	XM_004539038.3
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra tuberous sclerosis 2 (tsc2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004539038.3

ORF Insert Sequence:

ATGAATAAAC AACCAAGCAA GGAGAGTCTC AAGGACAAGG TGAAGGGGAT CTTTGGGCTC 
GGTACACAGC GTCCTCCGAA CAAACAGAGT GACACTAAAC CCTCCGAGTT TATCATTACG 
GCTGACATCA TCAAGGAGCT CCACCCAGAT TGCGGTCTGA GCAACCGTGT TCGCACTATG 
AACCACATCT GTGAGCTTGC CAAAATGAAA AAGTTTGAGG AGCATGCAGT GGAGGCTGTA 
TGGAAAAATG TGGAAGACAT GCTGACTCCA GAGCAGCCAC CAGAGGCCAG ACATGCTGTC 
CTGCAGCTGC TCAGGGCCAT TATACAGGGG CAGGGTGAGC GACTCGGCCC TCTGAGAGCC 
TACTTTTTCA AGGTAATCAG AGACTACCAG CCGTGCAATG AAGACCTTTC TGACAGACTG 
GAGGTGTTTA AGGCTTTAAC AGAGAATGGA AAGGATATCA CATACCTGGA AGAGGACATA 
GCACGCTTTG TCCTCCTGTG GATGGATATA GGCCTTACAT CTGACTTTCT CCATGTTCTT 
GTTAATCTGG TGAAATTCAA TAGCTGCTAC TTGGACCAAA ATGTTTCCAT CATGGTCCAG 
AAAATTTGCC TGCTGTGCAA CAGAACGACG TCTTCGACTG ATATTGAGGT GGCTCTGCAA 
GTGCTCGATG CAGTTGTGTG TTACAACTGC CTGCCCTCCG ACTCTTTAAC CGTTTTCATA 
ATCACACTCT GCCGTACGGT GAACGTCAAA GAGTTTTGCG AGTCCTGTTG GAAGTTAATG 
AGAAAGGTTT TGGGGACTCA CCTCGGCCAT AGTGCAATTT ACACCATGTG TCGCATTATG 
GAGGAAAGGG TGTATATGGA GGATGCACCG TTGTTAAGAG GAGCAGTTTT CTTTGTTGGA 
ATGGCGCTTT GGGGCGCACA CAGACTGCCT GCACTCAAAA ATACACCGAC GCTTGTTCTG 
CCATCTTTCT ACAAGGCCAT GTCATGTGCC AACGAGGTGG TCTCCTACGA AATTGTTCTT 
TCAATAACAA GACTCATCAA AAAGTACGGT AAAGAGCTAC AAGTGGTCAC ATGGGACATC 
CTACTCGGCA TCATAGAGCG ACTGTTGCAA CAGATTCAGA CAATAGGCAG TGCGGAACTA 
AAGGCCATTG TTTATGAACT GTTGACCACC GTGGAAGAGC TTTATGAACA AAACGGTTAC 
CACGGCTCCA CTGAGAAGTT TTTCAGCCTG GTGGAGAAAT GTGCTGATAA AAGACCAGAT 
GCATCAGTGC TGACACTTAT CTCCTACAGG GCTCAGTCTA TCCAGCCTGC TAAAGAAGGC 
TGGATTCAGA GCCTTCACCG GCTCATGGAG AAATTCTTCA GGAACGAGAC TCGCAGTGTG 
ATAAGAATCA AAGTGCTTCA TATCCTGTCC TTCGTTCTCA GTACCAACCG GCAGCTCTAT 
GAGGATGAGT TGATTGAAAT GGTGGTGATT CCCCAGCTCA GTGGGATTGC TGAAGACCGG 
GACTTGGCAG TTAGAAAACA GGCTACTCAG CTATTAGTAG ACCTCGCTGA GGGCTGCAAC 
ACGCACCACT TCACCAGCCT GCTGGACATC ATTGAACGGG TGTCTAGCCG CTCTCTGGTG 
TGTTCAGGGC CCCTAGAGGT TTCTGAGAGA GACCCCACAG CAGAGTCTCC TATGGAGGAC 
GTCAGGACTG CTATTCTGGG CCTGCTTGAA ATCCTGCAGA GCAAACTTTA CAGTCTGCCA 
GCCAGCCATG CGAGTCGTGT TTATGAGTTG CTTATCAGCC ACCTGCAGCT TCATTACAAG 
AACAAGTACT GTTCAACTAT CGCCTCCACT ATTAGACTAA AGGTGTTTGA CTTCTTCCTC 
ATGATGCGTG CAGACTCTCT TCACCGTCTT GGGGTCCCAA ACAAAGATGG TGTCATGAGA 
TTCAGCCCTT ATTGCTACTG TGATATAGGG GAACCAGAGA AACGTGTTGG TGAAAAGAAG 
CCAGCAGGTT CAACCTCTCC TCCTGCTGGC AGCCCTGCTC CTCCTGCTGC TCCTCCCAGT 
TCAGCAGCCT CAACACGCTC AGCCTACCTT TCCTATTCGC CTGCCTTCAG TGTCCTCCTG 
CAGTGCCTCA AGATGGAGAC GGATTGGAAG GTGCTGAAGC TGGTTCTTGA CAAGCTGCCA 
TGGATGCTGC AGTACAAAGT GTTGCTGCTC ACATCACCAT GCAACTTAGA TCAGCTGTGT 
TCTACACTCT GCTGCATGGT GACAGATCGT CTCATCTCAG AGCGATTGAA AAAGACTCCA 
GAGGGGTTTT CTCGCACAGA TGTTCAGCTG GCAGTGATTC CCGTTCTTAC AGCAATTACC 
TCCTACCACA ACTACTTGGA GCAGTCAAGA CAGAGAGAAC TGGTTCAGTG CCTCGAGACT 
GGGCTCATTT ACCGCTGTGC CAAGCAATGT GTGGTGGCCC TTACAATGTG CACGGTGGAG 
ATGCCTGATA TCATGATCAA GCTTCTTCCT GCTCTTATCG TCAAGCTCAC TCACATCTCT 
GCTACTGTTG CCATGGCATC TCCTATGCTC GAGTTTCTCT CCACCCTCGT GCGCCTTCCC 
CATCTGTATG CCAACTTTGT AGCTGAGCAG TATGTCAGCG TGTTTGCCAT CTCACTACCT 
TACACTAACC CCTCAAAGTT CAACCAGTAC ATTGTGTCCC TGGCCCACCA TGTGATTGCC 
ATGTGGTTCA TACGCTGCAG ACTACCCTTC CGCAAGGACT TTGTTCAGTA TATCACAAAG 
GGTTTGCGTT CCAACGCCCT TCTGCCATTC GATGACAGTC ATGAGCAGAG TCCGTTTCGT 
GCCCGAAGCA CCAGCCTCAA TGAGAGACCC AAGAGCTTGC GGGCAGCAAA AGTGGCAAAG 
GCAGCAGCAG CGGTAGCCAA TAGCAGCAGC TCTCCAGTTA AAGAGCTGAG GGACCTTTCA 
GCCATGGACG CTTTCCGCTC CCGCAGCATC AGCGTCTCCG AACATGCGGT CCGCAGGATG 
CACACCTCAA CCACCACATG CAGTCTGGGC TCTGCCGATG AAAATGCAGT AACTCAGGCC 
GACGAGGGGC TGAAAACTGT CCACCTAGAG CTCACAGAGA CTTGTCTTGA CATGATGGCC 
CGATATGTAT TTTCCAACTT CTCTGCACTT CCCAAGAGGT CTCCCATCGC AGAGTTCCTG 
TTAGCAGGAG GCCGCAGTAT GACCTGGTTG GTGGGCAACA AGTTGGTCAC CATAACAACC 
AGCGGAGGGG TCAGAACACA AGCGCTGCTG GGCCTGGACA TGGCTGATCG CCTGGGAGGA 
GGAGGAGGGG AATTGACCAG GTCAGACCCA TCACTACACA CCCGAATGAC CAAAGAGGCT 
CCAGCCAAAT TGGAGTCACA GTCAAGCCAG CAGCACAACA GAGCTGCACG CATACGAGTT 
CGCTCCATGT CAGGTGGTCA TGCTCTACGT GCCGGTCCAG CTCAGAGTCT CAGTCCTCTG 
GTGTCTCCCT CTGAGGGTGA GTTGACTGCT CCACTCTCTC CCTCCTCTGG TCCTGCTCTG 
GATAGTTTAG GTCCATCCTC TTCCACCTCT GCCACTCCAT CTGTGCCTCC TACTCTGAAA 
GACAACCCCA CCTTGGCTGA ATTTGCCCCA ATGCTCACAC AAGGCTGGGC AGAGATCTTC 
ATACGCAGAC CGTCAGGTAA CACAAGCTGG CTAATGTGTT TGGAGAACCC ACCTAGCCCT 
TTCTCATCTG AGCTTGGCAA CATGCCGCTG CAAGAGCTCT CCAGTGTTCT GATGGCAATG 
GAGGGAGTGA AGGAACCTCC CACTCAGACA GCCAGTGCTC CTGCTAGCAC AGCTGTCCCT 
GCTCCAGCTC CTGTCTCTGA GCCCCTAACC CAAACACACA GCAGTGTTGG AGGAAAGGCA 
AACCTGATTC AGCGCTCCAA CACAGTGGGT GGCTCTCTCT GCTCTCTGGG TATGGGCACT 
GCCCCCGCAG GCCCACCAGC CCCTGGCAGG TTGCACAGGA GCATTTCCTG GGCAGACTCT 
GTCGTGGTGC TGGAGGAGGG ATCAGGAATT GCTACAGCAA ACACGCCGCC TGAATGGCTG 
GGGAGTGCAG AGTTTGAGCC AATGTTGTCT GATCCAATCT TCATAGCTGC TGATAAGTTT 
ACAAAGACTC CTCCTGAAAC ACTCAGCAGG TCCTCCTCCA CCTCAAGTCA GGATGATGAA 
AAGTCAACCC TGGAAGAAGT AAGTGAGGGA GCAATTCCAA TCGATCAGCC CCCTTTTGGA 
CCATCCACTC CAGGAAGCCA GGGCCCTGAC CTTCCCTTCC AGACACACAC TCAGTCCCAG 
GGTCATGGAC TCAACAAGTC CAGCTCCTCT CCTGAGCTCC AGACATTACC TGAAGCTTTT 
GCCAAGGCTG CTATGGAGTC AGAAAGTGCG ATGGTTGACA CGTCACGGCC TAGAGCACCC 
TCAGACGGCA TGCCTCAACC CCAGCAGCCC CAGTTTGCGA AAGAAAAGGT AGAGGGAAAC 
ACTGGAGTGG ACACTAATGT AGCGGGAGGT CCAAGCCCAG CAGGTGAAGG CCAAGCTGTA 
TCGTCATCTC AGAGTGGAGG AGCGGGGATG AGGTTGTCTT TTCCACCAGC ACCAGTACAA 
TCTGCACCCA TCTCCCCCAG TGGAGGCCAC CGGCCCAGAG GTCACACAAT CTCAGTCTCA 
GCCCCCTCCT CCAGGAGGGA GAGGCGGGCG GAGAGGGACG CTTACCACAG TCGACCTGGT 
CCCAGCAACA CTGAGAAAAT CTCTGGACTG AGCCCCAGCT TTGTGTTTCT GCAGCTCTAC 
CACTCTCCTT TCTTTGGGAA TGAAGCCAAC AAGCCACTTC TGCTGCCCAA AACTCAGGTG 
ATTGACCGTG CAGTAAAGGT TCTGGACCAA ATGCCCCCTT ATGACACCCA TAAAATTGGG 
GTGGTGTTTG TAGGAGCTGG TCAGGTTAAC AATGAAGTCG CCATCTTGTC GAACGAGTAC 
GGTTCGAACC GCTACGCCTC TTTCCTCACA GGTCTTGGCA AATTAATCCA TCTGAAAGAC 
TGCGATCCAG ACCAGATTTT TCTTGGAGGG CTTGACCAGT ATGGAGATGA TGGAGAGTTC 
ACCTACTGCT GGCATGACGA CATCATGCAG GCTATCTTTC ACATAGCCAC ACTGATGCCA 
AACAGGGAGA GCGACAAGGG TTGCTGCAAC AAAAAGCGAC ACATTGGCAA CGATTTTGTC 
ATGGTGGTGT ACAATGACTC TGGAGAGGAG TACAAACTGG GCACAATTAA GGGTCAGTTT 
AATTTTGTCG AAGTCATTAT AAAACCACTG GATTATGAAT GTAACCTCGT TACCCTCCAG 
TGCCGCAAAG ACCTCGAGGG ATTAGTAGAC ACAACGGTGG CAAAAATTGT TTCAGATCGA 
AACCTTCCCC TGTTAGTCAG GCAGATGGCT CTGCATGCAA ATATGGCCTC ATTGGTGCAC 
CAGTACAGAG CTAACCCCTC TGACGCATAC GCCTCTAAGT GGCTAGCAAG GTTACGACAC 
ATCAAGAGGA TCAGGACCAG GGCTCAAGAA GCCATCCAGT CTCGCTCAAC TCCCGGGATT 
TCACTGACCC AAGGGCAGAG CAAACCTTTG TCACAGAGCA GTGCTCCAAC AAATGTGGAA 
GCCACCGGAC AACGAAAAAG ACTGGTGTCC ACTGTAGACG ATTTCACAGA TTTTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tsc2 ( XM_004539038.4 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004539095.1 Maylandia zebra TSC complex subunit 2 (tsc2), transcript variant X3, mRNA.

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CloneID	OMa18055
Clone ID Related Accession (Same CDS sequence)	XM_004539036.3 , XM_004539037.2 , XM_004539036.2 , XM_004539038.3 , XM_004539038.4
Accession Version	XM_004539038.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-29
Organism	Maylandia zebra(zebra mbuna)
Product	tuberin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014446893.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 30, 2017 this sequence version replaced XM_004539038.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAAAC AACCAAGCAA GGAGAGTCTC AAGGACAAGG TGAAGGGGAT CTTTGGGCTC 
GGTACACAGC GTCCTCCGAA CAAACAGAGT GACACTAAAC CCTCCGAGTT TATCATTACG 
GCTGACATCA TCAAGGAGCT CCACCCAGAT TGCGGTCTGA GCAACCGTGT TCGCACTATG 
AACCACATCT GTGAGCTTGC CAAAATGAAA AAGTTTGAGG AGCATGCAGT GGAGGCTGTA 
TGGAAAAATG TGGAAGACAT GCTGACTCCA GAGCAGCCAC CAGAGGCCAG ACATGCTGTC 
CTGCAGCTGC TCAGGGCCAT TATACAGGGG CAGGGTGAGC GACTCGGCCC TCTGAGAGCC 
TACTTTTTCA AGGTAATCAG AGACTACCAG CCGTGCAATG AAGACCTTTC TGACAGACTG 
GAGGTGTTTA AGGCTTTAAC AGAGAATGGA AAGGATATCA CATACCTGGA AGAGGACATA 
GCACGCTTTG TCCTCCTGTG GATGGATATA GGCCTTACAT CTGACTTTCT CCATGTTCTT 
GTTAATCTGG TGAAATTCAA TAGCTGCTAC TTGGACCAAA ATGTTTCCAT CATGGTCCAG 
AAAATTTGCC TGCTGTGCAA CAGAACGACG TCTTCGACTG ATATTGAGGT GGCTCTGCAA 
GTGCTCGATG CAGTTGTGTG TTACAACTGC CTGCCCTCCG ACTCTTTAAC CGTTTTCATA 
ATCACACTCT GCCGTACGGT GAACGTCAAA GAGTTTTGCG AGTCCTGTTG GAAGTTAATG 
AGAAAGGTTT TGGGGACTCA CCTCGGCCAT AGTGCAATTT ACACCATGTG TCGCATTATG 
GAGGAAAGGG TGTATATGGA GGATGCACCG TTGTTAAGAG GAGCAGTTTT CTTTGTTGGA 
ATGGCGCTTT GGGGCGCACA CAGACTGCCT GCACTCAAAA ATACACCGAC GCTTGTTCTG 
CCATCTTTCT ACAAGGCCAT GTCATGTGCC AACGAGGTGG TCTCCTACGA AATTGTTCTT 
TCAATAACAA GACTCATCAA AAAGTACGGT AAAGAGCTAC AAGTGGTCAC ATGGGACATC 
CTACTCGGCA TCATAGAGCG ACTGTTGCAA CAGATTCAGA CAATAGGCAG TGCGGAACTA 
AAGGCCATTG TTTATGAACT GTTGACCACC GTGGAAGAGC TTTATGAACA AAACGGTTAC 
CACGGCTCCA CTGAGAAGTT TTTCAGCCTG GTGGAGAAAT GTGCTGATAA AAGACCAGAT 
GCATCAGTGC TGACACTTAT CTCCTACAGG GCTCAGTCTA TCCAGCCTGC TAAAGAAGGC 
TGGATTCAGA GCCTTCACCG GCTCATGGAG AAATTCTTCA GGAACGAGAC TCGCAGTGTG 
ATAAGAATCA AAGTGCTTCA TATCCTGTCC TTCGTTCTCA GTACCAACCG GCAGCTCTAT 
GAGGATGAGT TGATTGAAAT GGTGGTGATT CCCCAGCTCA GTGGGATTGC TGAAGACCGG 
GACTTGGCAG TTAGAAAACA GGCTACTCAG CTATTAGTAG ACCTCGCTGA GGGCTGCAAC 
ACGCACCACT TCACCAGCCT GCTGGACATC ATTGAACGGG TGTCTAGCCG CTCTCTGGTG 
TGTTCAGGGC CCCTAGAGGT TTCTGAGAGA GACCCCACAG CAGAGTCTCC TATGGAGGAC 
GTCAGGACTG CTATTCTGGG CCTGCTTGAA ATCCTGCAGA GCAAACTTTA CAGTCTGCCA 
GCCAGCCATG CGAGTCGTGT TTATGAGTTG CTTATCAGCC ACCTGCAGCT TCATTACAAG 
AACAAGTACT GTTCAACTAT CGCCTCCACT ATTAGACTAA AGGTGTTTGA CTTCTTCCTC 
ATGATGCGTG CAGACTCTCT TCACCGTCTT GGGGTCCCAA ACAAAGATGG TGTCATGAGA 
TTCAGCCCTT ATTGCTACTG TGATATAGGG GAACCAGAGA AACGTGTTGG TGAAAAGAAG 
CCAGCAGGTT CAACCTCTCC TCCTGCTGGC AGCCCTGCTC CTCCTGCTGC TCCTCCCAGT 
TCAGCAGCCT CAACACGCTC AGCCTACCTT TCCTATTCGC CTGCCTTCAG TGTCCTCCTG 
CAGTGCCTCA AGATGGAGAC GGATTGGAAG GTGCTGAAGC TGGTTCTTGA CAAGCTGCCA 
TGGATGCTGC AGTACAAAGT GTTGCTGCTC ACATCACCAT GCAACTTAGA TCAGCTGTGT 
TCTACACTCT GCTGCATGGT GACAGATCGT CTCATCTCAG AGCGATTGAA AAAGACTCCA 
GAGGGGTTTT CTCGCACAGA TGTTCAGCTG GCAGTGATTC CCGTTCTTAC AGCAATTACC 
TCCTACCACA ACTACTTGGA GCAGTCAAGA CAGAGAGAAC TGGTTCAGTG CCTCGAGACT 
GGGCTCATTT ACCGCTGTGC CAAGCAATGT GTGGTGGCCC TTACAATGTG CACGGTGGAG 
ATGCCTGATA TCATGATCAA GCTTCTTCCT GCTCTTATCG TCAAGCTCAC TCACATCTCT 
GCTACTGTTG CCATGGCATC TCCTATGCTC GAGTTTCTCT CCACCCTCGT GCGCCTTCCC 
CATCTGTATG CCAACTTTGT AGCTGAGCAG TATGTCAGCG TGTTTGCCAT CTCACTACCT 
TACACTAACC CCTCAAAGTT CAACCAGTAC ATTGTGTCCC TGGCCCACCA TGTGATTGCC 
ATGTGGTTCA TACGCTGCAG ACTACCCTTC CGCAAGGACT TTGTTCAGTA TATCACAAAG 
GGTTTGCGTT CCAACGCCCT TCTGCCATTC GATGACAGTC ATGAGCAGAG TCCGTTTCGT 
GCCCGAAGCA CCAGCCTCAA TGAGAGACCC AAGAGCTTGC GGGCAGCAAA AGTGGCAAAG 
GCAGCAGCAG CGGTAGCCAA TAGCAGCAGC TCTCCAGTTA AAGAGCTGAG GGACCTTTCA 
GCCATGGACG CTTTCCGCTC CCGCAGCATC AGCGTCTCCG AACATGCGGT CCGCAGGATG 
CACACCTCAA CCACCACATG CAGTCTGGGC TCTGCCGATG AAAATGCAGT AACTCAGGCC 
GACGAGGGGC TGAAAACTGT CCACCTAGAG CTCACAGAGA CTTGTCTTGA CATGATGGCC 
CGATATGTAT TTTCCAACTT CTCTGCACTT CCCAAGAGGT CTCCCATCGC AGAGTTCCTG 
TTAGCAGGAG GCCGCAGTAT GACCTGGTTG GTGGGCAACA AGTTGGTCAC CATAACAACC 
AGCGGAGGGG TCAGAACACA AGCGCTGCTG GGCCTGGACA TGGCTGATCG CCTGGGAGGA 
GGAGGAGGGG AATTGACCAG GTCAGACCCA TCACTACACA CCCGAATGAC CAAAGAGGCT 
CCAGCCAAAT TGGAGTCACA GTCAAGCCAG CAGCACAACA GAGCTGCACG CATACGAGTT 
CGCTCCATGT CAGGTGGTCA TGCTCTACGT GCCGGTCCAG CTCAGAGTCT CAGTCCTCTG 
GTGTCTCCCT CTGAGGGTGA GTTGACTGCT CCACTCTCTC CCTCCTCTGG TCCTGCTCTG 
GATAGTTTAG GTCCATCCTC TTCCACCTCT GCCACTCCAT CTGTGCCTCC TACTCTGAAA 
GACAACCCCA CCTTGGCTGA ATTTGCCCCA ATGCTCACAC AAGGCTGGGC AGAGATCTTC 
ATACGCAGAC CGTCAGGTAA CACAAGCTGG CTAATGTGTT TGGAGAACCC ACCTAGCCCT 
TTCTCATCTG AGCTTGGCAA CATGCCGCTG CAAGAGCTCT CCAGTGTTCT GATGGCAATG 
GAGGGAGTGA AGGAACCTCC CACTCAGACA GCCAGTGCTC CTGCTAGCAC AGCTGTCCCT 
GCTCCAGCTC CTGTCTCTGA GCCCCTAACC CAAACACACA GCAGTGTTGG AGGAAAGGCA 
AACCTGATTC AGCGCTCCAA CACAGTGGGT GGCTCTCTCT GCTCTCTGGG TATGGGCACT 
GCCCCCGCAG GCCCACCAGC CCCTGGCAGG TTGCACAGGA GCATTTCCTG GGCAGACTCT 
GTCGTGGTGC TGGAGGAGGG ATCAGGAATT GCTACAGCAA ACACGCCGCC TGAATGGCTG 
GGGAGTGCAG AGTTTGAGCC AATGTTGTCT GATCCAATCT TCATAGCTGC TGATAAGTTT 
ACAAAGACTC CTCCTGAAAC ACTCAGCAGG TCCTCCTCCA CCTCAAGTCA GGATGATGAA 
AAGTCAACCC TGGAAGAAGT AAGTGAGGGA GCAATTCCAA TCGATCAGCC CCCTTTTGGA 
CCATCCACTC CAGGAAGCCA GGGCCCTGAC CTTCCCTTCC AGACACACAC TCAGTCCCAG 
GGTCATGGAC TCAACAAGTC CAGCTCCTCT CCTGAGCTCC AGACATTACC TGAAGCTTTT 
GCCAAGGCTG CTATGGAGTC AGAAAGTGCG ATGGTTGACA CGTCACGGCC TAGAGCACCC 
TCAGACGGCA TGCCTCAACC CCAGCAGCCC CAGTTTGCGA AAGAAAAGGT AGAGGGAAAC 
ACTGGAGTGG ACACTAATGT AGCGGGAGGT CCAAGCCCAG CAGGTGAAGG CCAAGCTGTA 
TCGTCATCTC AGAGTGGAGG AGCGGGGATG AGGTTGTCTT TTCCACCAGC ACCAGTACAA 
TCTGCACCCA TCTCCCCCAG TGGAGGCCAC CGGCCCAGAG GTCACACAAT CTCAGTCTCA 
GCCCCCTCCT CCAGGAGGGA GAGGCGGGCG GAGAGGGACG CTTACCACAG TCGACCTGGT 
CCCAGCAACA CTGAGAAAAT CTCTGGACTG AGCCCCAGCT TTGTGTTTCT GCAGCTCTAC 
CACTCTCCTT TCTTTGGGAA TGAAGCCAAC AAGCCACTTC TGCTGCCCAA AACTCAGGTG 
ATTGACCGTG CAGTAAAGGT TCTGGACCAA ATGCCCCCTT ATGACACCCA TAAAATTGGG 
GTGGTGTTTG TAGGAGCTGG TCAGGTTAAC AATGAAGTCG CCATCTTGTC GAACGAGTAC 
GGTTCGAACC GCTACGCCTC TTTCCTCACA GGTCTTGGCA AATTAATCCA TCTGAAAGAC 
TGCGATCCAG ACCAGATTTT TCTTGGAGGG CTTGACCAGT ATGGAGATGA TGGAGAGTTC 
ACCTACTGCT GGCATGACGA CATCATGCAG GCTATCTTTC ACATAGCCAC ACTGATGCCA 
AACAGGGAGA GCGACAAGGG TTGCTGCAAC AAAAAGCGAC ACATTGGCAA CGATTTTGTC 
ATGGTGGTGT ACAATGACTC TGGAGAGGAG TACAAACTGG GCACAATTAA GGGTCAGTTT 
AATTTTGTCG AAGTCATTAT AAAACCACTG GATTATGAAT GTAACCTCGT TACCCTCCAG 
TGCCGCAAAG ACCTCGAGGG ATTAGTAGAC ACAACGGTGG CAAAAATTGT TTCAGATCGA 
AACCTTCCCC TGTTAGTCAG GCAGATGGCT CTGCATGCAA ATATGGCCTC ATTGGTGCAC 
CAGTACAGAG CTAACCCCTC TGACGCATAC GCCTCTAAGT GGCTAGCAAG GTTACGACAC 
ATCAAGAGGA TCAGGACCAG GGCTCAAGAA GCCATCCAGT CTCGCTCAAC TCCCGGGATT 
TCACTGACCC AAGGGCAGAG CAAACCTTTG TCACAGAGCA GTGCTCCAAC AAATGTGGAA 
GCCACCGGAC AACGAAAAAG ACTGGTGTCC ACTGTAGACG ATTTCACAGA TTTTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004539095.1
CDS	71..5770
Translation	MNKQPSKESLKDKVKGIFGLGTQRPPNKQSDTKPSEFIITADIIKELHPDCGLSNRVRTMNHICELAKMKKFEEHAVEAVWKNVEDMLTPEQPPEARHAVLQLLRAIIQGQGERLGPLRAYFFKVIRDYQPCNEDLSDRLEVFKALTENGKDITYLEEDIARFVLLWMDIGLTSDFLHVLVNLVKFNSCYLDQNVSIMVQKICLLCNRTTSSTDIEVALQVLDAVVCYNCLPSDSLTVFIITLCRTVNVKEFCESCWKLMRKVLGTHLGHSAIYTMCRIMEERVYMEDAPLLRGAVFFVGMALWGAHRLPALKNTPTLVLPSFYKAMSCANEVVSYEIVLSITRLIKKYGKELQVVTWDILLGIIERLLQQIQTIGSAELKAIVYELLTTVEELYEQNGYHGSTEKFFSLVEKCADKRPDASVLTLISYRAQSIQPAKEGWIQSLHRLMEKFFRNETRSVIRIKVLHILSFVLSTNRQLYEDELIEMVVIPQLSGIAEDRDLAVRKQATQLLVDLAEGCNTHHFTSLLDIIERVSSRSLVCSGPLEVSERDPTAESPMEDVRTAILGLLEILQSKLYSLPASHASRVYELLISHLQLHYKNKYCSTIASTIRLKVFDFFLMMRADSLHRLGVPNKDGVMRFSPYCYCDIGEPEKRVGEKKPAGSTSPPAGSPAPPAAPPSSAASTRSAYLSYSPAFSVLLQCLKMETDWKVLKLVLDKLPWMLQYKVLLLTSPCNLDQLCSTLCCMVTDRLISERLKKTPEGFSRTDVQLAVIPVLTAITSYHNYLEQSRQRELVQCLETGLIYRCAKQCVVALTMCTVEMPDIMIKLLPALIVKLTHISATVAMASPMLEFLSTLVRLPHLYANFVAEQYVSVFAISLPYTNPSKFNQYIVSLAHHVIAMWFIRCRLPFRKDFVQYITKGLRSNALLPFDDSHEQSPFRARSTSLNERPKSLRAAKVAKAAAAVANSSSSPVKELRDLSAMDAFRSRSISVSEHAVRRMHTSTTTCSLGSADENAVTQADEGLKTVHLELTETCLDMMARYVFSNFSALPKRSPIAEFLLAGGRSMTWLVGNKLVTITTSGGVRTQALLGLDMADRLGGGGGELTRSDPSLHTRMTKEAPAKLESQSSQQHNRAARIRVRSMSGGHALRAGPAQSLSPLVSPSEGELTAPLSPSSGPALDSLGPSSSTSATPSVPPTLKDNPTLAEFAPMLTQGWAEIFIRRPSGNTSWLMCLENPPSPFSSELGNMPLQELSSVLMAMEGVKEPPTQTASAPASTAVPAPAPVSEPLTQTHSSVGGKANLIQRSNTVGGSLCSLGMGTAPAGPPAPGRLHRSISWADSVVVLEEGSGIATANTPPEWLGSAEFEPMLSDPIFIAADKFTKTPPETLSRSSSTSSQDDEKSTLEEVSEGAIPIDQPPFGPSTPGSQGPDLPFQTHTQSQGHGLNKSSSSPELQTLPEAFAKAAMESESAMVDTSRPRAPSDGMPQPQQPQFAKEKVEGNTGVDTNVAGGPSPAGEGQAVSSSQSGGAGMRLSFPPAPVQSAPISPSGGHRPRGHTISVSAPSSRRERRAERDAYHSRPGPSNTEKISGLSPSFVFLQLYHSPFFGNEANKPLLLPKTQVIDRAVKVLDQMPPYDTHKIGVVFVGAGQVNNEVAILSNEYGSNRYASFLTGLGKLIHLKDCDPDQIFLGGLDQYGDDGEFTYCWHDDIMQAIFHIATLMPNRESDKGCCNKKRHIGNDFVMVVYNDSGEEYKLGTIKGQFNFVEVIIKPLDYECNLVTLQCRKDLEGLVDTTVAKIVSDRNLPLLVRQMALHANMASLVHQYRANPSDAYASKWLARLRHIKRIRTRAQEAIQSRSTPGISLTQGQSKPLSQSSAPTNVEATGQRKRLVSTVDDFTDFV

Target ORF information:

RefSeq Version	XM_004539038.4
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra TSC complex subunit 2 (tsc2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004539038.4

ORF Insert Sequence:

ATGAATAAAC AACCAAGCAA GGAGAGTCTC AAGGACAAGG TGAAGGGGAT CTTTGGGCTC 
GGTACACAGC GTCCTCCGAA CAAACAGAGT GACACTAAAC CCTCCGAGTT TATCATTACG 
GCTGACATCA TCAAGGAGCT CCACCCAGAT TGCGGTCTGA GCAACCGTGT TCGCACTATG 
AACCACATCT GTGAGCTTGC CAAAATGAAA AAGTTTGAGG AGCATGCAGT GGAGGCTGTA 
TGGAAAAATG TGGAAGACAT GCTGACTCCA GAGCAGCCAC CAGAGGCCAG ACATGCTGTC 
CTGCAGCTGC TCAGGGCCAT TATACAGGGG CAGGGTGAGC GACTCGGCCC TCTGAGAGCC 
TACTTTTTCA AGGTAATCAG AGACTACCAG CCGTGCAATG AAGACCTTTC TGACAGACTG 
GAGGTGTTTA AGGCTTTAAC AGAGAATGGA AAGGATATCA CATACCTGGA AGAGGACATA 
GCACGCTTTG TCCTCCTGTG GATGGATATA GGCCTTACAT CTGACTTTCT CCATGTTCTT 
GTTAATCTGG TGAAATTCAA TAGCTGCTAC TTGGACCAAA ATGTTTCCAT CATGGTCCAG 
AAAATTTGCC TGCTGTGCAA CAGAACGACG TCTTCGACTG ATATTGAGGT GGCTCTGCAA 
GTGCTCGATG CAGTTGTGTG TTACAACTGC CTGCCCTCCG ACTCTTTAAC CGTTTTCATA 
ATCACACTCT GCCGTACGGT GAACGTCAAA GAGTTTTGCG AGTCCTGTTG GAAGTTAATG 
AGAAAGGTTT TGGGGACTCA CCTCGGCCAT AGTGCAATTT ACACCATGTG TCGCATTATG 
GAGGAAAGGG TGTATATGGA GGATGCACCG TTGTTAAGAG GAGCAGTTTT CTTTGTTGGA 
ATGGCGCTTT GGGGCGCACA CAGACTGCCT GCACTCAAAA ATACACCGAC GCTTGTTCTG 
CCATCTTTCT ACAAGGCCAT GTCATGTGCC AACGAGGTGG TCTCCTACGA AATTGTTCTT 
TCAATAACAA GACTCATCAA AAAGTACGGT AAAGAGCTAC AAGTGGTCAC ATGGGACATC 
CTACTCGGCA TCATAGAGCG ACTGTTGCAA CAGATTCAGA CAATAGGCAG TGCGGAACTA 
AAGGCCATTG TTTATGAACT GTTGACCACC GTGGAAGAGC TTTATGAACA AAACGGTTAC 
CACGGCTCCA CTGAGAAGTT TTTCAGCCTG GTGGAGAAAT GTGCTGATAA AAGACCAGAT 
GCATCAGTGC TGACACTTAT CTCCTACAGG GCTCAGTCTA TCCAGCCTGC TAAAGAAGGC 
TGGATTCAGA GCCTTCACCG GCTCATGGAG AAATTCTTCA GGAACGAGAC TCGCAGTGTG 
ATAAGAATCA AAGTGCTTCA TATCCTGTCC TTCGTTCTCA GTACCAACCG GCAGCTCTAT 
GAGGATGAGT TGATTGAAAT GGTGGTGATT CCCCAGCTCA GTGGGATTGC TGAAGACCGG 
GACTTGGCAG TTAGAAAACA GGCTACTCAG CTATTAGTAG ACCTCGCTGA GGGCTGCAAC 
ACGCACCACT TCACCAGCCT GCTGGACATC ATTGAACGGG TGTCTAGCCG CTCTCTGGTG 
TGTTCAGGGC CCCTAGAGGT TTCTGAGAGA GACCCCACAG CAGAGTCTCC TATGGAGGAC 
GTCAGGACTG CTATTCTGGG CCTGCTTGAA ATCCTGCAGA GCAAACTTTA CAGTCTGCCA 
GCCAGCCATG CGAGTCGTGT TTATGAGTTG CTTATCAGCC ACCTGCAGCT TCATTACAAG 
AACAAGTACT GTTCAACTAT CGCCTCCACT ATTAGACTAA AGGTGTTTGA CTTCTTCCTC 
ATGATGCGTG CAGACTCTCT TCACCGTCTT GGGGTCCCAA ACAAAGATGG TGTCATGAGA 
TTCAGCCCTT ATTGCTACTG TGATATAGGG GAACCAGAGA AACGTGTTGG TGAAAAGAAG 
CCAGCAGGTT CAACCTCTCC TCCTGCTGGC AGCCCTGCTC CTCCTGCTGC TCCTCCCAGT 
TCAGCAGCCT CAACACGCTC AGCCTACCTT TCCTATTCGC CTGCCTTCAG TGTCCTCCTG 
CAGTGCCTCA AGATGGAGAC GGATTGGAAG GTGCTGAAGC TGGTTCTTGA CAAGCTGCCA 
TGGATGCTGC AGTACAAAGT GTTGCTGCTC ACATCACCAT GCAACTTAGA TCAGCTGTGT 
TCTACACTCT GCTGCATGGT GACAGATCGT CTCATCTCAG AGCGATTGAA AAAGACTCCA 
GAGGGGTTTT CTCGCACAGA TGTTCAGCTG GCAGTGATTC CCGTTCTTAC AGCAATTACC 
TCCTACCACA ACTACTTGGA GCAGTCAAGA CAGAGAGAAC TGGTTCAGTG CCTCGAGACT 
GGGCTCATTT ACCGCTGTGC CAAGCAATGT GTGGTGGCCC TTACAATGTG CACGGTGGAG 
ATGCCTGATA TCATGATCAA GCTTCTTCCT GCTCTTATCG TCAAGCTCAC TCACATCTCT 
GCTACTGTTG CCATGGCATC TCCTATGCTC GAGTTTCTCT CCACCCTCGT GCGCCTTCCC 
CATCTGTATG CCAACTTTGT AGCTGAGCAG TATGTCAGCG TGTTTGCCAT CTCACTACCT 
TACACTAACC CCTCAAAGTT CAACCAGTAC ATTGTGTCCC TGGCCCACCA TGTGATTGCC 
ATGTGGTTCA TACGCTGCAG ACTACCCTTC CGCAAGGACT TTGTTCAGTA TATCACAAAG 
GGTTTGCGTT CCAACGCCCT TCTGCCATTC GATGACAGTC ATGAGCAGAG TCCGTTTCGT 
GCCCGAAGCA CCAGCCTCAA TGAGAGACCC AAGAGCTTGC GGGCAGCAAA AGTGGCAAAG 
GCAGCAGCAG CGGTAGCCAA TAGCAGCAGC TCTCCAGTTA AAGAGCTGAG GGACCTTTCA 
GCCATGGACG CTTTCCGCTC CCGCAGCATC AGCGTCTCCG AACATGCGGT CCGCAGGATG 
CACACCTCAA CCACCACATG CAGTCTGGGC TCTGCCGATG AAAATGCAGT AACTCAGGCC 
GACGAGGGGC TGAAAACTGT CCACCTAGAG CTCACAGAGA CTTGTCTTGA CATGATGGCC 
CGATATGTAT TTTCCAACTT CTCTGCACTT CCCAAGAGGT CTCCCATCGC AGAGTTCCTG 
TTAGCAGGAG GCCGCAGTAT GACCTGGTTG GTGGGCAACA AGTTGGTCAC CATAACAACC 
AGCGGAGGGG TCAGAACACA AGCGCTGCTG GGCCTGGACA TGGCTGATCG CCTGGGAGGA 
GGAGGAGGGG AATTGACCAG GTCAGACCCA TCACTACACA CCCGAATGAC CAAAGAGGCT 
CCAGCCAAAT TGGAGTCACA GTCAAGCCAG CAGCACAACA GAGCTGCACG CATACGAGTT 
CGCTCCATGT CAGGTGGTCA TGCTCTACGT GCCGGTCCAG CTCAGAGTCT CAGTCCTCTG 
GTGTCTCCCT CTGAGGGTGA GTTGACTGCT CCACTCTCTC CCTCCTCTGG TCCTGCTCTG 
GATAGTTTAG GTCCATCCTC TTCCACCTCT GCCACTCCAT CTGTGCCTCC TACTCTGAAA 
GACAACCCCA CCTTGGCTGA ATTTGCCCCA ATGCTCACAC AAGGCTGGGC AGAGATCTTC 
ATACGCAGAC CGTCAGGTAA CACAAGCTGG CTAATGTGTT TGGAGAACCC ACCTAGCCCT 
TTCTCATCTG AGCTTGGCAA CATGCCGCTG CAAGAGCTCT CCAGTGTTCT GATGGCAATG 
GAGGGAGTGA AGGAACCTCC CACTCAGACA GCCAGTGCTC CTGCTAGCAC AGCTGTCCCT 
GCTCCAGCTC CTGTCTCTGA GCCCCTAACC CAAACACACA GCAGTGTTGG AGGAAAGGCA 
AACCTGATTC AGCGCTCCAA CACAGTGGGT GGCTCTCTCT GCTCTCTGGG TATGGGCACT 
GCCCCCGCAG GCCCACCAGC CCCTGGCAGG TTGCACAGGA GCATTTCCTG GGCAGACTCT 
GTCGTGGTGC TGGAGGAGGG ATCAGGAATT GCTACAGCAA ACACGCCGCC TGAATGGCTG 
GGGAGTGCAG AGTTTGAGCC AATGTTGTCT GATCCAATCT TCATAGCTGC TGATAAGTTT 
ACAAAGACTC CTCCTGAAAC ACTCAGCAGG TCCTCCTCCA CCTCAAGTCA GGATGATGAA 
AAGTCAACCC TGGAAGAAGT AAGTGAGGGA GCAATTCCAA TCGATCAGCC CCCTTTTGGA 
CCATCCACTC CAGGAAGCCA GGGCCCTGAC CTTCCCTTCC AGACACACAC TCAGTCCCAG 
GGTCATGGAC TCAACAAGTC CAGCTCCTCT CCTGAGCTCC AGACATTACC TGAAGCTTTT 
GCCAAGGCTG CTATGGAGTC AGAAAGTGCG ATGGTTGACA CGTCACGGCC TAGAGCACCC 
TCAGACGGCA TGCCTCAACC CCAGCAGCCC CAGTTTGCGA AAGAAAAGGT AGAGGGAAAC 
ACTGGAGTGG ACACTAATGT AGCGGGAGGT CCAAGCCCAG CAGGTGAAGG CCAAGCTGTA 
TCGTCATCTC AGAGTGGAGG AGCGGGGATG AGGTTGTCTT TTCCACCAGC ACCAGTACAA 
TCTGCACCCA TCTCCCCCAG TGGAGGCCAC CGGCCCAGAG GTCACACAAT CTCAGTCTCA 
GCCCCCTCCT CCAGGAGGGA GAGGCGGGCG GAGAGGGACG CTTACCACAG TCGACCTGGT 
CCCAGCAACA CTGAGAAAAT CTCTGGACTG AGCCCCAGCT TTGTGTTTCT GCAGCTCTAC 
CACTCTCCTT TCTTTGGGAA TGAAGCCAAC AAGCCACTTC TGCTGCCCAA AACTCAGGTG 
ATTGACCGTG CAGTAAAGGT TCTGGACCAA ATGCCCCCTT ATGACACCCA TAAAATTGGG 
GTGGTGTTTG TAGGAGCTGG TCAGGTTAAC AATGAAGTCG CCATCTTGTC GAACGAGTAC 
GGTTCGAACC GCTACGCCTC TTTCCTCACA GGTCTTGGCA AATTAATCCA TCTGAAAGAC 
TGCGATCCAG ACCAGATTTT TCTTGGAGGG CTTGACCAGT ATGGAGATGA TGGAGAGTTC 
ACCTACTGCT GGCATGACGA CATCATGCAG GCTATCTTTC ACATAGCCAC ACTGATGCCA 
AACAGGGAGA GCGACAAGGG TTGCTGCAAC AAAAAGCGAC ACATTGGCAA CGATTTTGTC 
ATGGTGGTGT ACAATGACTC TGGAGAGGAG TACAAACTGG GCACAATTAA GGGTCAGTTT 
AATTTTGTCG AAGTCATTAT AAAACCACTG GATTATGAAT GTAACCTCGT TACCCTCCAG 
TGCCGCAAAG ACCTCGAGGG ATTAGTAGAC ACAACGGTGG CAAAAATTGT TTCAGATCGA 
AACCTTCCCC TGTTAGTCAG GCAGATGGCT CTGCATGCAA ATATGGCCTC ATTGGTGCAC 
CAGTACAGAG CTAACCCCTC TGACGCATAC GCCTCTAAGT GGCTAGCAAG GTTACGACAC 
ATCAAGAGGA TCAGGACCAG GGCTCAAGAA GCCATCCAGT CTCGCTCAAC TCCCGGGATT 
TCACTGACCC AAGGGCAGAG CAAACCTTTG TCACAGAGCA GTGCTCCAAC AAATGTGGAA 
GCCACCGGAC AACGAAAAAG ACTGGTGTCC ACTGTAGACG ATTTCACAGA TTTTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tsc2 ( XM_004539039.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004539096.1 Maylandia zebra tuberous sclerosis 2 (tsc2), transcript variant X4, mRNA.

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CloneID	OMa18056
Clone ID Related Accession (Same CDS sequence)	XM_004539039.2 , XM_004539039.3
Accession Version	XM_004539039.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5610bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-10-15
Organism	Maylandia zebra(Zebra mbuna)
Product	tuberin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014446893.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_004539039.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAAAC AACCAAGCAA GGAGAGTCTC AAGGACAAGG TGAAGGGGAT CTTTGGGCTC 
GGTACACAGC GTCCTCCGAA CAAACAGAGT GACACTAAAC CCTCCGAGTT TATCATTACG 
GCTGACATCA TCAAGGAGCT CCACCCAGAT TGCGGTCTGA GCAACCGTGT TCGCACTATG 
AACCACATCT GTGAGCTTGC CAAAATGAAA AAGTTTGAGG AGCATGCAGT GGAGGCTGTA 
TGGAAAAATG TGGAAGACAT GCTGACTCCA GAGCAGCCAC CAGAGGCCAG ACATGCTGTC 
CTGCAGCTGC TCAGGGCCAT TATACAGGGG CAGGGTGAGC GACTCGGCCC TCTGAGAGCC 
TACTTTTTCA AGGTAATCAG AGACTACCAG CCGTGCAATG AAGACCTTTC TGACAGACTG 
GAGGTGTTTA AGGCTTTAAC AGAGAATGGA AAGGATATCA CATACCTGGA AGAGGACATA 
GCACGCTTTG TCCTCCTGTG GATGGATATA GGCCTTACAT CTGACTTTCT CCATGTTCTT 
GTTAATCTGG TGAAATTCAA TAGCTGCTAC TTGGACCAAA ATGTTTCCAT CATGGTCCAG 
AAAATTTGCC TGCTGTGCAA CAGAACGACG TCTTCGACTG ATATTGAGGT GGCTCTGCAA 
GTGCTCGATG CAGTTGTGTG TTACAACTGC CTGCCCTCCG ACTCTTTAAC CGTTTTCATA 
ATCACACTCT GCCGTACGGT GAACGTCAAA GAGTTTTGCG AGTCCTGTTG GAAGTTAATG 
AGAAAGGTTT TGGGGACTCA CCTCGGCCAT AGTGCAATTT ACACCATGTG TCGCATTATG 
GAGGAAAGGG TGTATATGGA GGATGCACCG TTGTTAAGAG GAGCAGTTTT CTTTGTTGGA 
ATGGCGCTTT GGGGCGCACA CAGACTGCCT GCACTCAAAA ATACACCGAC GCTTGTTCTG 
CCATCTTTCT ACAAGGCCAT GTCATGTGCC AACGAGGTGG TCTCCTACGA AATTGTTCTT 
TCAATAACAA GACTCATCAA AAAGTACGGT AAAGAGCTAC AAGTGGTCAC ATGGGACATC 
CTACTCGGCA TCATAGAGCG ACTGTTGCAA CAGATTCAGA CAATAGGCAG TGCGGAACTA 
AAGGCCATTG TTTATGAACT GTTGACCACC GTGGAAGAGC TTTATGAACA AAACGGTTAC 
CACGGCTCCA CTGAGAAGTT TTTCAGCCTG GTGGAGAAAT GTGCTGATAA AAGACCAGAT 
GCATCAGTGC TGACACTTAT CTCCTACAGG GCTCAGTCTA TCCAGCCTGC TAAAGAAGGC 
TGGATTCAGA GCCTTCACCG GCTCATGGAG AAATTCTTCA GGAACGAGAC TCGCAGTGTG 
ATAAGAATCA AAGTGCTTCA TATCCTGTCC TTCGTTCTCA GTACCAACCG GCAGCTCTAT 
GAGGATGAGT TGATTGAAAT GGTGGTGATT CCCCAGCTCA GTGGGATTGC TGAAGACCGG 
GACTTGGCAG TTAGAAAACA GGCTACTCAG CTATTAGTAG ACCTCGCTGA GGGCTGCAAC 
ACGCACCACT TCACCAGCCT GCTGGACATC ATTGAACGGG TGTCTAGCCG CTCTCTGGTG 
TGTTCAGGGC CCCTAGAGGT TTCTGAGAGA GACCCCACAG CAGAGTCTCC TATGGAGGAC 
GTCAGGACTG CTATTCTGGG CCTGCTTGAA ATCCTGCAGA GCAAACTTTA CAGTCTGCCA 
GCCAGCCATG CGAGTCGTGT TTATGAGTTG CTTATCAGCC ACCTGCAGCT TCATTACAAG 
AACAAGTACT GTTCAACTAT CGCCTCCACT ATTAGACTAA AGGTGTTTGA CTTCTTCCTC 
ATGATGCGTG CAGACTCTCT TCACCGTCTT GGGGTCCCAA ACAAAGATGG TGTCATGAGA 
TTCAGCCCTT ATTGCTACTG TGATATAGGG GAACCAGAGA AACGTGTTGG TGAAAAGAAG 
CCAGCAGGTT CAACCTCTCC TCCTGCTGGC AGCCCTGCTC CTCCTGCTGC TCCTCCCAGT 
TCAGCAGCCT CAACACGCTC AGCCTACCTT TCCTATTCGC CTGCCTTCAG TGTCCTCCTG 
CAGTGCCTCA AGATGGAGAC GGATTGGAAG GTGCTGAAGC TGGTTCTTGA CAAGCTGCCA 
TGGATGCTGC AGTACAAAGT GTTGCTGCTC ACATCACCAT GCAACTTAGA TCAGCTGTGT 
TCTACACTCT GCTGCATGGT GACAGATCGT CTCATCTCAG AGCGATTGAA AAAGACTCCA 
GAGGGGTTTT CTCGCACAGA TGTTCAGCTG GCAGTGATTC CCGTTCTTAC AGCAATTACC 
TCCTACCACA ACTACTTGGA GCAGTCAAGA CAGAGAGAAC TGGTTCAGTG CCTCGAGACT 
GGGCTCATTT ACCGCTGTGC CAAGCAATGT GTGGTGGCCC TTACAATGTG CACGGTGGAG 
ATGCCTGATA TCATGATCAA GCTTCTTCCT GCTCTTATCG TCAAGCTCAC TCACATCTCT 
GCTACTGTTG CCATGGCATC TCCTATGCTC GAGTTTCTCT CCACCCTCGT GCGCCTTCCC 
CATCTGTATG CCAACTTTGT AGCTGAGCAG TATGTCAGCG TGTTTGCCAT CTCACTACCT 
TACACTAACC CCTCAAAGTT CAACCAGTAC ATTGTGTCCC TGGCCCACCA TGTGATTGCC 
ATGTGGTTCA TACGCTGCAG ACTACCCTTC CGCAAGGACT TTGTTCAGTA TATCACAAAG 
GGTTTGCGTT CCAACGCCCT TCTGCCATTC GATGACAGTC ATGAGCAGAG TCCGTTTCGT 
GCCCGAAGCA CCAGCCTCAA TGAGAGACCC AAGAGCTTGC GGGCAGCAAA AGTGGCAAAG 
GCAGCAGCAG CGGTAGCCAA TAGCAGCAGC TCTCCAGTTA AAGAGCTGAG GGACCTTTCA 
GCCATGGACG CTTTCCGCTC CCGCAGCATC AGCGTCTCCG AACATGCGGT CCGCAGGATG 
CACACCTCAA CCACCACATG CAGTCTGGGC TCTGCCGATG AAAATGCAGT AACTCAGGCC 
GACGAGGGGC TGAAAACTGT CCACCTAGAG CTCACAGAGA CTTGTCTTGA CATGATGGCC 
CGATATGTAT TTTCCAACTT CTCTGCACTT CCCAAGAGGT CTCCCATCGC AGAGTTCCTG 
TTAGCAGGAG GCCGCAGTAT GACCTGGTTG GTGGGCAACA AGTTGGTCAC CATAACAACC 
AGCGGAGGGG TCAGAACACA AGCGCTGCTG GGCCTGGACA TGGCTGATCG CCTGGGAGGA 
GGAGGAGGGG AATTGACCAG GTCAGACCCA TCACTACACA CCCGAATGAC CAAAGAGGCT 
CCAGCCAAAT TGGAGTCACA GTCAAGCCAG CAGCACAACA GAGCTGCACG CATACGAGTT 
CGCTCCATGT CAGGTGGTCA TGCTCTACGT GCCGGTCCAG CTCAGAGTCT CAGTCCTCTG 
GTGTCTCCCT CTGAGGGTGA GTTGACTGCT CCACTCTCTC CCTCCTCTGG TCCTGCTCTG 
GATAGTTTAG GTCCATCCTC TTCCACCTCT GCCACTCCAT CTGTGCCTCC TACTCTGAAA 
GACAACCCCA CCTTGGCTGA ATTTGCCCCA ATGCTCACAC AAGGCTGGGC AGAGATCTTC 
ATACGCAGAC CGTCAGGTAA CACAAGCTGG CTAATGTGTT TGGAGAACCC ACCTAGCCCT 
TTCTCATCTG AGCTTGGCAA CATGCCGCTG CAAGAGCTCT CCAGTGTTCT GATGGCAATG 
GAGGGAGTGA AGGAACCTCC CACTCAGACA GCCAGTGCTC CTGCTAGCAC AGCTGTCCCT 
GCTCCAGCTC CTGTCTCTGA GCCCCTAACC CAAACACACA GCAGTGTTGG AGGAAAGGCA 
AACCTGATTC AGCGCTCCAA CACAGACTCT GTCGTGGTGC TGGAGGAGGG ATCAGGAATT 
GCTACAGCAA ACACGCCGCC TGAATGGCTG GGGAGTGCAG AGTTTGAGCC AATGTTGTCT 
GATCCAATCT TCATAGCTGC TGATAAGTTT ACAAAGACTC CTCCTGAAAC ACTCAGCAGG 
TCCTCCTCCA CCTCAAGTCA GGATGATGAA AAGTCAACCC TGGAAGAAGT AAGTGAGGGA 
GCAATTCCAA TCGATCAGCC CCCTTTTGGA CCATCCACTC CAGGAAGCCA GGGCCCTGAC 
CTTCCCTTCC AGACACACAC TCAGTCCCAG GGTCATGGAC TCAACAAGTC CAGCTCCTCT 
CCTGAGCTCC AGACATTACC TGAAGCTTTT GCCAAGGCTG CTATGGAGTC AGAAAGTGCG 
ATGGTTGACA CGTCACGGCC TAGAGCACCC TCAGACGGCA TGCCTCAACC CCAGCAGCCC 
CAGTTTGCGA AAGAAAAGGT AGAGGGAAAC ACTGGAGTGG ACACTAATGT AGCGGGAGGT 
CCAAGCCCAG CAGGTGAAGG CCAAGCTGTA TCGTCATCTC AGAGTGGAGG AGCGGGGATG 
AGGTTGTCTT TTCCACCAGC ACCAGTACAA TCTGCACCCA TCTCCCCCAG TGGAGGCCAC 
CGGCCCAGAG GTCACACAAT CTCAGTCTCA GCCCCCTCCT CCAGGAGGGA GAGGCGGGCG 
GAGAGGGACG CTTACCACAG TCGACCTGGT CCCAGCAACA CTGAGAAAAT CTCTGGACTG 
AGCCCCAGCT TTGTGTTTCT GCAGCTCTAC CACTCTCCTT TCTTTGGGAA TGAAGCCAAC 
AAGCCACTTC TGCTGCCCAA AACTCAGGTG ATTGACCGTG CAGTAAAGGT TCTGGACCAA 
ATGCCCCCTT ATGACACCCA TAAAATTGGG GTGGTGTTTG TAGGAGCTGG TCAGGTTAAC 
AATGAAGTCG CCATCTTGTC GAACGAGTAC GGTTCGAACC GCTACGCCTC TTTCCTCACA 
GGTCTTGGCA AATTAATCCA TCTGAAAGAC TGCGATCCAG ACCAGATTTT TCTTGGAGGG 
CTTGACCAGT ATGGAGATGA TGGAGAGTTC ACCTACTGCT GGCATGACGA CATCATGCAG 
GCTATCTTTC ACATAGCCAC ACTGATGCCA AACAGGGAGA GCGACAAGGG TTGCTGCAAC 
AAAAAGCGAC ACATTGGCAA CGATTTTGTC ATGGTGGTGT ACAATGACTC TGGAGAGGAG 
TACAAACTGG GCACAATTAA GGGTCAGTTT AATTTTGTCG AAGTCATTAT AAAACCACTG 
GATTATGAAT GTAACCTCGT TACCCTCCAG TGCCGCAAAG ACCTCGAGGG ATTAGTAGAC 
ACAACGGTGG CAAAAATTGT TTCAGATCGA AACCTTCCCC TGTTAGTCAG GCAGATGGCT 
CTGCATGCAA ATATGGCCTC ATTGGTGCAC CAGTACAGAG CTAACCCCTC TGACGCATAC 
GCCTCTAAGT GGCTAGCAAG GTTACGACAC ATCAAGAGGA TCAGGACCAG GGCTCAAGAA 
GCCATCCAGT CTCGCTCAAC TCCCGGGATT TCACTGACCC AAGGGCAGAG CAAACCTTTG 
TCACAGAGCA GTGCTCCAAC AAATGTGGAA GCCACCGGAC AACGAAAAAG ACTGGTGTCC 
ACTGTAGACG ATTTCACAGA TTTTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004539096.1
CDS	195..5804
Translation	MNKQPSKESLKDKVKGIFGLGTQRPPNKQSDTKPSEFIITADIIKELHPDCGLSNRVRTMNHICELAKMKKFEEHAVEAVWKNVEDMLTPEQPPEARHAVLQLLRAIIQGQGERLGPLRAYFFKVIRDYQPCNEDLSDRLEVFKALTENGKDITYLEEDIARFVLLWMDIGLTSDFLHVLVNLVKFNSCYLDQNVSIMVQKICLLCNRTTSSTDIEVALQVLDAVVCYNCLPSDSLTVFIITLCRTVNVKEFCESCWKLMRKVLGTHLGHSAIYTMCRIMEERVYMEDAPLLRGAVFFVGMALWGAHRLPALKNTPTLVLPSFYKAMSCANEVVSYEIVLSITRLIKKYGKELQVVTWDILLGIIERLLQQIQTIGSAELKAIVYELLTTVEELYEQNGYHGSTEKFFSLVEKCADKRPDASVLTLISYRAQSIQPAKEGWIQSLHRLMEKFFRNETRSVIRIKVLHILSFVLSTNRQLYEDELIEMVVIPQLSGIAEDRDLAVRKQATQLLVDLAEGCNTHHFTSLLDIIERVSSRSLVCSGPLEVSERDPTAESPMEDVRTAILGLLEILQSKLYSLPASHASRVYELLISHLQLHYKNKYCSTIASTIRLKVFDFFLMMRADSLHRLGVPNKDGVMRFSPYCYCDIGEPEKRVGEKKPAGSTSPPAGSPAPPAAPPSSAASTRSAYLSYSPAFSVLLQCLKMETDWKVLKLVLDKLPWMLQYKVLLLTSPCNLDQLCSTLCCMVTDRLISERLKKTPEGFSRTDVQLAVIPVLTAITSYHNYLEQSRQRELVQCLETGLIYRCAKQCVVALTMCTVEMPDIMIKLLPALIVKLTHISATVAMASPMLEFLSTLVRLPHLYANFVAEQYVSVFAISLPYTNPSKFNQYIVSLAHHVIAMWFIRCRLPFRKDFVQYITKGLRSNALLPFDDSHEQSPFRARSTSLNERPKSLRAAKVAKAAAAVANSSSSPVKELRDLSAMDAFRSRSISVSEHAVRRMHTSTTTCSLGSADENAVTQADEGLKTVHLELTETCLDMMARYVFSNFSALPKRSPIAEFLLAGGRSMTWLVGNKLVTITTSGGVRTQALLGLDMADRLGGGGGELTRSDPSLHTRMTKEAPAKLESQSSQQHNRAARIRVRSMSGGHALRAGPAQSLSPLVSPSEGELTAPLSPSSGPALDSLGPSSSTSATPSVPPTLKDNPTLAEFAPMLTQGWAEIFIRRPSGNTSWLMCLENPPSPFSSELGNMPLQELSSVLMAMEGVKEPPTQTASAPASTAVPAPAPVSEPLTQTHSSVGGKANLIQRSNTDSVVVLEEGSGIATANTPPEWLGSAEFEPMLSDPIFIAADKFTKTPPETLSRSSSTSSQDDEKSTLEEVSEGAIPIDQPPFGPSTPGSQGPDLPFQTHTQSQGHGLNKSSSSPELQTLPEAFAKAAMESESAMVDTSRPRAPSDGMPQPQQPQFAKEKVEGNTGVDTNVAGGPSPAGEGQAVSSSQSGGAGMRLSFPPAPVQSAPISPSGGHRPRGHTISVSAPSSRRERRAERDAYHSRPGPSNTEKISGLSPSFVFLQLYHSPFFGNEANKPLLLPKTQVIDRAVKVLDQMPPYDTHKIGVVFVGAGQVNNEVAILSNEYGSNRYASFLTGLGKLIHLKDCDPDQIFLGGLDQYGDDGEFTYCWHDDIMQAIFHIATLMPNRESDKGCCNKKRHIGNDFVMVVYNDSGEEYKLGTIKGQFNFVEVIIKPLDYECNLVTLQCRKDLEGLVDTTVAKIVSDRNLPLLVRQMALHANMASLVHQYRANPSDAYASKWLARLRHIKRIRTRAQEAIQSRSTPGISLTQGQSKPLSQSSAPTNVEATGQRKRLVSTVDDFTDFV

Target ORF information:

RefSeq Version	XM_004539039.2
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra tuberous sclerosis 2 (tsc2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004539039.2

ORF Insert Sequence:

ATGAATAAAC AACCAAGCAA GGAGAGTCTC AAGGACAAGG TGAAGGGGAT CTTTGGGCTC 
GGTACACAGC GTCCTCCGAA CAAACAGAGT GACACTAAAC CCTCCGAGTT TATCATTACG 
GCTGACATCA TCAAGGAGCT CCACCCAGAT TGCGGTCTGA GCAACCGTGT TCGCACTATG 
AACCACATCT GTGAGCTTGC CAAAATGAAA AAGTTTGAGG AGCATGCAGT GGAGGCTGTA 
TGGAAAAATG TGGAAGACAT GCTGACTCCA GAGCAGCCAC CAGAGGCCAG ACATGCTGTC 
CTGCAGCTGC TCAGGGCCAT TATACAGGGG CAGGGTGAGC GACTCGGCCC TCTGAGAGCC 
TACTTTTTCA AGGTAATCAG AGACTACCAG CCGTGCAATG AAGACCTTTC TGACAGACTG 
GAGGTGTTTA AGGCTTTAAC AGAGAATGGA AAGGATATCA CATACCTGGA AGAGGACATA 
GCACGCTTTG TCCTCCTGTG GATGGATATA GGCCTTACAT CTGACTTTCT CCATGTTCTT 
GTTAATCTGG TGAAATTCAA TAGCTGCTAC TTGGACCAAA ATGTTTCCAT CATGGTCCAG 
AAAATTTGCC TGCTGTGCAA CAGAACGACG TCTTCGACTG ATATTGAGGT GGCTCTGCAA 
GTGCTCGATG CAGTTGTGTG TTACAACTGC CTGCCCTCCG ACTCTTTAAC CGTTTTCATA 
ATCACACTCT GCCGTACGGT GAACGTCAAA GAGTTTTGCG AGTCCTGTTG GAAGTTAATG 
AGAAAGGTTT TGGGGACTCA CCTCGGCCAT AGTGCAATTT ACACCATGTG TCGCATTATG 
GAGGAAAGGG TGTATATGGA GGATGCACCG TTGTTAAGAG GAGCAGTTTT CTTTGTTGGA 
ATGGCGCTTT GGGGCGCACA CAGACTGCCT GCACTCAAAA ATACACCGAC GCTTGTTCTG 
CCATCTTTCT ACAAGGCCAT GTCATGTGCC AACGAGGTGG TCTCCTACGA AATTGTTCTT 
TCAATAACAA GACTCATCAA AAAGTACGGT AAAGAGCTAC AAGTGGTCAC ATGGGACATC 
CTACTCGGCA TCATAGAGCG ACTGTTGCAA CAGATTCAGA CAATAGGCAG TGCGGAACTA 
AAGGCCATTG TTTATGAACT GTTGACCACC GTGGAAGAGC TTTATGAACA AAACGGTTAC 
CACGGCTCCA CTGAGAAGTT TTTCAGCCTG GTGGAGAAAT GTGCTGATAA AAGACCAGAT 
GCATCAGTGC TGACACTTAT CTCCTACAGG GCTCAGTCTA TCCAGCCTGC TAAAGAAGGC 
TGGATTCAGA GCCTTCACCG GCTCATGGAG AAATTCTTCA GGAACGAGAC TCGCAGTGTG 
ATAAGAATCA AAGTGCTTCA TATCCTGTCC TTCGTTCTCA GTACCAACCG GCAGCTCTAT 
GAGGATGAGT TGATTGAAAT GGTGGTGATT CCCCAGCTCA GTGGGATTGC TGAAGACCGG 
GACTTGGCAG TTAGAAAACA GGCTACTCAG CTATTAGTAG ACCTCGCTGA GGGCTGCAAC 
ACGCACCACT TCACCAGCCT GCTGGACATC ATTGAACGGG TGTCTAGCCG CTCTCTGGTG 
TGTTCAGGGC CCCTAGAGGT TTCTGAGAGA GACCCCACAG CAGAGTCTCC TATGGAGGAC 
GTCAGGACTG CTATTCTGGG CCTGCTTGAA ATCCTGCAGA GCAAACTTTA CAGTCTGCCA 
GCCAGCCATG CGAGTCGTGT TTATGAGTTG CTTATCAGCC ACCTGCAGCT TCATTACAAG 
AACAAGTACT GTTCAACTAT CGCCTCCACT ATTAGACTAA AGGTGTTTGA CTTCTTCCTC 
ATGATGCGTG CAGACTCTCT TCACCGTCTT GGGGTCCCAA ACAAAGATGG TGTCATGAGA 
TTCAGCCCTT ATTGCTACTG TGATATAGGG GAACCAGAGA AACGTGTTGG TGAAAAGAAG 
CCAGCAGGTT CAACCTCTCC TCCTGCTGGC AGCCCTGCTC CTCCTGCTGC TCCTCCCAGT 
TCAGCAGCCT CAACACGCTC AGCCTACCTT TCCTATTCGC CTGCCTTCAG TGTCCTCCTG 
CAGTGCCTCA AGATGGAGAC GGATTGGAAG GTGCTGAAGC TGGTTCTTGA CAAGCTGCCA 
TGGATGCTGC AGTACAAAGT GTTGCTGCTC ACATCACCAT GCAACTTAGA TCAGCTGTGT 
TCTACACTCT GCTGCATGGT GACAGATCGT CTCATCTCAG AGCGATTGAA AAAGACTCCA 
GAGGGGTTTT CTCGCACAGA TGTTCAGCTG GCAGTGATTC CCGTTCTTAC AGCAATTACC 
TCCTACCACA ACTACTTGGA GCAGTCAAGA CAGAGAGAAC TGGTTCAGTG CCTCGAGACT 
GGGCTCATTT ACCGCTGTGC CAAGCAATGT GTGGTGGCCC TTACAATGTG CACGGTGGAG 
ATGCCTGATA TCATGATCAA GCTTCTTCCT GCTCTTATCG TCAAGCTCAC TCACATCTCT 
GCTACTGTTG CCATGGCATC TCCTATGCTC GAGTTTCTCT CCACCCTCGT GCGCCTTCCC 
CATCTGTATG CCAACTTTGT AGCTGAGCAG TATGTCAGCG TGTTTGCCAT CTCACTACCT 
TACACTAACC CCTCAAAGTT CAACCAGTAC ATTGTGTCCC TGGCCCACCA TGTGATTGCC 
ATGTGGTTCA TACGCTGCAG ACTACCCTTC CGCAAGGACT TTGTTCAGTA TATCACAAAG 
GGTTTGCGTT CCAACGCCCT TCTGCCATTC GATGACAGTC ATGAGCAGAG TCCGTTTCGT 
GCCCGAAGCA CCAGCCTCAA TGAGAGACCC AAGAGCTTGC GGGCAGCAAA AGTGGCAAAG 
GCAGCAGCAG CGGTAGCCAA TAGCAGCAGC TCTCCAGTTA AAGAGCTGAG GGACCTTTCA 
GCCATGGACG CTTTCCGCTC CCGCAGCATC AGCGTCTCCG AACATGCGGT CCGCAGGATG 
CACACCTCAA CCACCACATG CAGTCTGGGC TCTGCCGATG AAAATGCAGT AACTCAGGCC 
GACGAGGGGC TGAAAACTGT CCACCTAGAG CTCACAGAGA CTTGTCTTGA CATGATGGCC 
CGATATGTAT TTTCCAACTT CTCTGCACTT CCCAAGAGGT CTCCCATCGC AGAGTTCCTG 
TTAGCAGGAG GCCGCAGTAT GACCTGGTTG GTGGGCAACA AGTTGGTCAC CATAACAACC 
AGCGGAGGGG TCAGAACACA AGCGCTGCTG GGCCTGGACA TGGCTGATCG CCTGGGAGGA 
GGAGGAGGGG AATTGACCAG GTCAGACCCA TCACTACACA CCCGAATGAC CAAAGAGGCT 
CCAGCCAAAT TGGAGTCACA GTCAAGCCAG CAGCACAACA GAGCTGCACG CATACGAGTT 
CGCTCCATGT CAGGTGGTCA TGCTCTACGT GCCGGTCCAG CTCAGAGTCT CAGTCCTCTG 
GTGTCTCCCT CTGAGGGTGA GTTGACTGCT CCACTCTCTC CCTCCTCTGG TCCTGCTCTG 
GATAGTTTAG GTCCATCCTC TTCCACCTCT GCCACTCCAT CTGTGCCTCC TACTCTGAAA 
GACAACCCCA CCTTGGCTGA ATTTGCCCCA ATGCTCACAC AAGGCTGGGC AGAGATCTTC 
ATACGCAGAC CGTCAGGTAA CACAAGCTGG CTAATGTGTT TGGAGAACCC ACCTAGCCCT 
TTCTCATCTG AGCTTGGCAA CATGCCGCTG CAAGAGCTCT CCAGTGTTCT GATGGCAATG 
GAGGGAGTGA AGGAACCTCC CACTCAGACA GCCAGTGCTC CTGCTAGCAC AGCTGTCCCT 
GCTCCAGCTC CTGTCTCTGA GCCCCTAACC CAAACACACA GCAGTGTTGG AGGAAAGGCA 
AACCTGATTC AGCGCTCCAA CACAGACTCT GTCGTGGTGC TGGAGGAGGG ATCAGGAATT 
GCTACAGCAA ACACGCCGCC TGAATGGCTG GGGAGTGCAG AGTTTGAGCC AATGTTGTCT 
GATCCAATCT TCATAGCTGC TGATAAGTTT ACAAAGACTC CTCCTGAAAC ACTCAGCAGG 
TCCTCCTCCA CCTCAAGTCA GGATGATGAA AAGTCAACCC TGGAAGAAGT AAGTGAGGGA 
GCAATTCCAA TCGATCAGCC CCCTTTTGGA CCATCCACTC CAGGAAGCCA GGGCCCTGAC 
CTTCCCTTCC AGACACACAC TCAGTCCCAG GGTCATGGAC TCAACAAGTC CAGCTCCTCT 
CCTGAGCTCC AGACATTACC TGAAGCTTTT GCCAAGGCTG CTATGGAGTC AGAAAGTGCG 
ATGGTTGACA CGTCACGGCC TAGAGCACCC TCAGACGGCA TGCCTCAACC CCAGCAGCCC 
CAGTTTGCGA AAGAAAAGGT AGAGGGAAAC ACTGGAGTGG ACACTAATGT AGCGGGAGGT 
CCAAGCCCAG CAGGTGAAGG CCAAGCTGTA TCGTCATCTC AGAGTGGAGG AGCGGGGATG 
AGGTTGTCTT TTCCACCAGC ACCAGTACAA TCTGCACCCA TCTCCCCCAG TGGAGGCCAC 
CGGCCCAGAG GTCACACAAT CTCAGTCTCA GCCCCCTCCT CCAGGAGGGA GAGGCGGGCG 
GAGAGGGACG CTTACCACAG TCGACCTGGT CCCAGCAACA CTGAGAAAAT CTCTGGACTG 
AGCCCCAGCT TTGTGTTTCT GCAGCTCTAC CACTCTCCTT TCTTTGGGAA TGAAGCCAAC 
AAGCCACTTC TGCTGCCCAA AACTCAGGTG ATTGACCGTG CAGTAAAGGT TCTGGACCAA 
ATGCCCCCTT ATGACACCCA TAAAATTGGG GTGGTGTTTG TAGGAGCTGG TCAGGTTAAC 
AATGAAGTCG CCATCTTGTC GAACGAGTAC GGTTCGAACC GCTACGCCTC TTTCCTCACA 
GGTCTTGGCA AATTAATCCA TCTGAAAGAC TGCGATCCAG ACCAGATTTT TCTTGGAGGG 
CTTGACCAGT ATGGAGATGA TGGAGAGTTC ACCTACTGCT GGCATGACGA CATCATGCAG 
GCTATCTTTC ACATAGCCAC ACTGATGCCA AACAGGGAGA GCGACAAGGG TTGCTGCAAC 
AAAAAGCGAC ACATTGGCAA CGATTTTGTC ATGGTGGTGT ACAATGACTC TGGAGAGGAG 
TACAAACTGG GCACAATTAA GGGTCAGTTT AATTTTGTCG AAGTCATTAT AAAACCACTG 
GATTATGAAT GTAACCTCGT TACCCTCCAG TGCCGCAAAG ACCTCGAGGG ATTAGTAGAC 
ACAACGGTGG CAAAAATTGT TTCAGATCGA AACCTTCCCC TGTTAGTCAG GCAGATGGCT 
CTGCATGCAA ATATGGCCTC ATTGGTGCAC CAGTACAGAG CTAACCCCTC TGACGCATAC 
GCCTCTAAGT GGCTAGCAAG GTTACGACAC ATCAAGAGGA TCAGGACCAG GGCTCAAGAA 
GCCATCCAGT CTCGCTCAAC TCCCGGGATT TCACTGACCC AAGGGCAGAG CAAACCTTTG 
TCACAGAGCA GTGCTCCAAC AAATGTGGAA GCCACCGGAC AACGAAAAAG ACTGGTGTCC 
ACTGTAGACG ATTTCACAGA TTTTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tsc2 ( XM_004539039.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004539096.1 Maylandia zebra TSC complex subunit 2 (tsc2), transcript variant X4, mRNA.

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CloneID	OMa18056
Clone ID Related Accession (Same CDS sequence)	XM_004539039.2 , XM_004539039.3
Accession Version	XM_004539039.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5610bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-29
Organism	Maylandia zebra(zebra mbuna)
Product	tuberin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014446893.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 30, 2017 this sequence version replaced XM_004539039.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAAAC AACCAAGCAA GGAGAGTCTC AAGGACAAGG TGAAGGGGAT CTTTGGGCTC 
GGTACACAGC GTCCTCCGAA CAAACAGAGT GACACTAAAC CCTCCGAGTT TATCATTACG 
GCTGACATCA TCAAGGAGCT CCACCCAGAT TGCGGTCTGA GCAACCGTGT TCGCACTATG 
AACCACATCT GTGAGCTTGC CAAAATGAAA AAGTTTGAGG AGCATGCAGT GGAGGCTGTA 
TGGAAAAATG TGGAAGACAT GCTGACTCCA GAGCAGCCAC CAGAGGCCAG ACATGCTGTC 
CTGCAGCTGC TCAGGGCCAT TATACAGGGG CAGGGTGAGC GACTCGGCCC TCTGAGAGCC 
TACTTTTTCA AGGTAATCAG AGACTACCAG CCGTGCAATG AAGACCTTTC TGACAGACTG 
GAGGTGTTTA AGGCTTTAAC AGAGAATGGA AAGGATATCA CATACCTGGA AGAGGACATA 
GCACGCTTTG TCCTCCTGTG GATGGATATA GGCCTTACAT CTGACTTTCT CCATGTTCTT 
GTTAATCTGG TGAAATTCAA TAGCTGCTAC TTGGACCAAA ATGTTTCCAT CATGGTCCAG 
AAAATTTGCC TGCTGTGCAA CAGAACGACG TCTTCGACTG ATATTGAGGT GGCTCTGCAA 
GTGCTCGATG CAGTTGTGTG TTACAACTGC CTGCCCTCCG ACTCTTTAAC CGTTTTCATA 
ATCACACTCT GCCGTACGGT GAACGTCAAA GAGTTTTGCG AGTCCTGTTG GAAGTTAATG 
AGAAAGGTTT TGGGGACTCA CCTCGGCCAT AGTGCAATTT ACACCATGTG TCGCATTATG 
GAGGAAAGGG TGTATATGGA GGATGCACCG TTGTTAAGAG GAGCAGTTTT CTTTGTTGGA 
ATGGCGCTTT GGGGCGCACA CAGACTGCCT GCACTCAAAA ATACACCGAC GCTTGTTCTG 
CCATCTTTCT ACAAGGCCAT GTCATGTGCC AACGAGGTGG TCTCCTACGA AATTGTTCTT 
TCAATAACAA GACTCATCAA AAAGTACGGT AAAGAGCTAC AAGTGGTCAC ATGGGACATC 
CTACTCGGCA TCATAGAGCG ACTGTTGCAA CAGATTCAGA CAATAGGCAG TGCGGAACTA 
AAGGCCATTG TTTATGAACT GTTGACCACC GTGGAAGAGC TTTATGAACA AAACGGTTAC 
CACGGCTCCA CTGAGAAGTT TTTCAGCCTG GTGGAGAAAT GTGCTGATAA AAGACCAGAT 
GCATCAGTGC TGACACTTAT CTCCTACAGG GCTCAGTCTA TCCAGCCTGC TAAAGAAGGC 
TGGATTCAGA GCCTTCACCG GCTCATGGAG AAATTCTTCA GGAACGAGAC TCGCAGTGTG 
ATAAGAATCA AAGTGCTTCA TATCCTGTCC TTCGTTCTCA GTACCAACCG GCAGCTCTAT 
GAGGATGAGT TGATTGAAAT GGTGGTGATT CCCCAGCTCA GTGGGATTGC TGAAGACCGG 
GACTTGGCAG TTAGAAAACA GGCTACTCAG CTATTAGTAG ACCTCGCTGA GGGCTGCAAC 
ACGCACCACT TCACCAGCCT GCTGGACATC ATTGAACGGG TGTCTAGCCG CTCTCTGGTG 
TGTTCAGGGC CCCTAGAGGT TTCTGAGAGA GACCCCACAG CAGAGTCTCC TATGGAGGAC 
GTCAGGACTG CTATTCTGGG CCTGCTTGAA ATCCTGCAGA GCAAACTTTA CAGTCTGCCA 
GCCAGCCATG CGAGTCGTGT TTATGAGTTG CTTATCAGCC ACCTGCAGCT TCATTACAAG 
AACAAGTACT GTTCAACTAT CGCCTCCACT ATTAGACTAA AGGTGTTTGA CTTCTTCCTC 
ATGATGCGTG CAGACTCTCT TCACCGTCTT GGGGTCCCAA ACAAAGATGG TGTCATGAGA 
TTCAGCCCTT ATTGCTACTG TGATATAGGG GAACCAGAGA AACGTGTTGG TGAAAAGAAG 
CCAGCAGGTT CAACCTCTCC TCCTGCTGGC AGCCCTGCTC CTCCTGCTGC TCCTCCCAGT 
TCAGCAGCCT CAACACGCTC AGCCTACCTT TCCTATTCGC CTGCCTTCAG TGTCCTCCTG 
CAGTGCCTCA AGATGGAGAC GGATTGGAAG GTGCTGAAGC TGGTTCTTGA CAAGCTGCCA 
TGGATGCTGC AGTACAAAGT GTTGCTGCTC ACATCACCAT GCAACTTAGA TCAGCTGTGT 
TCTACACTCT GCTGCATGGT GACAGATCGT CTCATCTCAG AGCGATTGAA AAAGACTCCA 
GAGGGGTTTT CTCGCACAGA TGTTCAGCTG GCAGTGATTC CCGTTCTTAC AGCAATTACC 
TCCTACCACA ACTACTTGGA GCAGTCAAGA CAGAGAGAAC TGGTTCAGTG CCTCGAGACT 
GGGCTCATTT ACCGCTGTGC CAAGCAATGT GTGGTGGCCC TTACAATGTG CACGGTGGAG 
ATGCCTGATA TCATGATCAA GCTTCTTCCT GCTCTTATCG TCAAGCTCAC TCACATCTCT 
GCTACTGTTG CCATGGCATC TCCTATGCTC GAGTTTCTCT CCACCCTCGT GCGCCTTCCC 
CATCTGTATG CCAACTTTGT AGCTGAGCAG TATGTCAGCG TGTTTGCCAT CTCACTACCT 
TACACTAACC CCTCAAAGTT CAACCAGTAC ATTGTGTCCC TGGCCCACCA TGTGATTGCC 
ATGTGGTTCA TACGCTGCAG ACTACCCTTC CGCAAGGACT TTGTTCAGTA TATCACAAAG 
GGTTTGCGTT CCAACGCCCT TCTGCCATTC GATGACAGTC ATGAGCAGAG TCCGTTTCGT 
GCCCGAAGCA CCAGCCTCAA TGAGAGACCC AAGAGCTTGC GGGCAGCAAA AGTGGCAAAG 
GCAGCAGCAG CGGTAGCCAA TAGCAGCAGC TCTCCAGTTA AAGAGCTGAG GGACCTTTCA 
GCCATGGACG CTTTCCGCTC CCGCAGCATC AGCGTCTCCG AACATGCGGT CCGCAGGATG 
CACACCTCAA CCACCACATG CAGTCTGGGC TCTGCCGATG AAAATGCAGT AACTCAGGCC 
GACGAGGGGC TGAAAACTGT CCACCTAGAG CTCACAGAGA CTTGTCTTGA CATGATGGCC 
CGATATGTAT TTTCCAACTT CTCTGCACTT CCCAAGAGGT CTCCCATCGC AGAGTTCCTG 
TTAGCAGGAG GCCGCAGTAT GACCTGGTTG GTGGGCAACA AGTTGGTCAC CATAACAACC 
AGCGGAGGGG TCAGAACACA AGCGCTGCTG GGCCTGGACA TGGCTGATCG CCTGGGAGGA 
GGAGGAGGGG AATTGACCAG GTCAGACCCA TCACTACACA CCCGAATGAC CAAAGAGGCT 
CCAGCCAAAT TGGAGTCACA GTCAAGCCAG CAGCACAACA GAGCTGCACG CATACGAGTT 
CGCTCCATGT CAGGTGGTCA TGCTCTACGT GCCGGTCCAG CTCAGAGTCT CAGTCCTCTG 
GTGTCTCCCT CTGAGGGTGA GTTGACTGCT CCACTCTCTC CCTCCTCTGG TCCTGCTCTG 
GATAGTTTAG GTCCATCCTC TTCCACCTCT GCCACTCCAT CTGTGCCTCC TACTCTGAAA 
GACAACCCCA CCTTGGCTGA ATTTGCCCCA ATGCTCACAC AAGGCTGGGC AGAGATCTTC 
ATACGCAGAC CGTCAGGTAA CACAAGCTGG CTAATGTGTT TGGAGAACCC ACCTAGCCCT 
TTCTCATCTG AGCTTGGCAA CATGCCGCTG CAAGAGCTCT CCAGTGTTCT GATGGCAATG 
GAGGGAGTGA AGGAACCTCC CACTCAGACA GCCAGTGCTC CTGCTAGCAC AGCTGTCCCT 
GCTCCAGCTC CTGTCTCTGA GCCCCTAACC CAAACACACA GCAGTGTTGG AGGAAAGGCA 
AACCTGATTC AGCGCTCCAA CACAGACTCT GTCGTGGTGC TGGAGGAGGG ATCAGGAATT 
GCTACAGCAA ACACGCCGCC TGAATGGCTG GGGAGTGCAG AGTTTGAGCC AATGTTGTCT 
GATCCAATCT TCATAGCTGC TGATAAGTTT ACAAAGACTC CTCCTGAAAC ACTCAGCAGG 
TCCTCCTCCA CCTCAAGTCA GGATGATGAA AAGTCAACCC TGGAAGAAGT AAGTGAGGGA 
GCAATTCCAA TCGATCAGCC CCCTTTTGGA CCATCCACTC CAGGAAGCCA GGGCCCTGAC 
CTTCCCTTCC AGACACACAC TCAGTCCCAG GGTCATGGAC TCAACAAGTC CAGCTCCTCT 
CCTGAGCTCC AGACATTACC TGAAGCTTTT GCCAAGGCTG CTATGGAGTC AGAAAGTGCG 
ATGGTTGACA CGTCACGGCC TAGAGCACCC TCAGACGGCA TGCCTCAACC CCAGCAGCCC 
CAGTTTGCGA AAGAAAAGGT AGAGGGAAAC ACTGGAGTGG ACACTAATGT AGCGGGAGGT 
CCAAGCCCAG CAGGTGAAGG CCAAGCTGTA TCGTCATCTC AGAGTGGAGG AGCGGGGATG 
AGGTTGTCTT TTCCACCAGC ACCAGTACAA TCTGCACCCA TCTCCCCCAG TGGAGGCCAC 
CGGCCCAGAG GTCACACAAT CTCAGTCTCA GCCCCCTCCT CCAGGAGGGA GAGGCGGGCG 
GAGAGGGACG CTTACCACAG TCGACCTGGT CCCAGCAACA CTGAGAAAAT CTCTGGACTG 
AGCCCCAGCT TTGTGTTTCT GCAGCTCTAC CACTCTCCTT TCTTTGGGAA TGAAGCCAAC 
AAGCCACTTC TGCTGCCCAA AACTCAGGTG ATTGACCGTG CAGTAAAGGT TCTGGACCAA 
ATGCCCCCTT ATGACACCCA TAAAATTGGG GTGGTGTTTG TAGGAGCTGG TCAGGTTAAC 
AATGAAGTCG CCATCTTGTC GAACGAGTAC GGTTCGAACC GCTACGCCTC TTTCCTCACA 
GGTCTTGGCA AATTAATCCA TCTGAAAGAC TGCGATCCAG ACCAGATTTT TCTTGGAGGG 
CTTGACCAGT ATGGAGATGA TGGAGAGTTC ACCTACTGCT GGCATGACGA CATCATGCAG 
GCTATCTTTC ACATAGCCAC ACTGATGCCA AACAGGGAGA GCGACAAGGG TTGCTGCAAC 
AAAAAGCGAC ACATTGGCAA CGATTTTGTC ATGGTGGTGT ACAATGACTC TGGAGAGGAG 
TACAAACTGG GCACAATTAA GGGTCAGTTT AATTTTGTCG AAGTCATTAT AAAACCACTG 
GATTATGAAT GTAACCTCGT TACCCTCCAG TGCCGCAAAG ACCTCGAGGG ATTAGTAGAC 
ACAACGGTGG CAAAAATTGT TTCAGATCGA AACCTTCCCC TGTTAGTCAG GCAGATGGCT 
CTGCATGCAA ATATGGCCTC ATTGGTGCAC CAGTACAGAG CTAACCCCTC TGACGCATAC 
GCCTCTAAGT GGCTAGCAAG GTTACGACAC ATCAAGAGGA TCAGGACCAG GGCTCAAGAA 
GCCATCCAGT CTCGCTCAAC TCCCGGGATT TCACTGACCC AAGGGCAGAG CAAACCTTTG 
TCACAGAGCA GTGCTCCAAC AAATGTGGAA GCCACCGGAC AACGAAAAAG ACTGGTGTCC 
ACTGTAGACG ATTTCACAGA TTTTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004539096.1
CDS	198..5807
Translation	MNKQPSKESLKDKVKGIFGLGTQRPPNKQSDTKPSEFIITADIIKELHPDCGLSNRVRTMNHICELAKMKKFEEHAVEAVWKNVEDMLTPEQPPEARHAVLQLLRAIIQGQGERLGPLRAYFFKVIRDYQPCNEDLSDRLEVFKALTENGKDITYLEEDIARFVLLWMDIGLTSDFLHVLVNLVKFNSCYLDQNVSIMVQKICLLCNRTTSSTDIEVALQVLDAVVCYNCLPSDSLTVFIITLCRTVNVKEFCESCWKLMRKVLGTHLGHSAIYTMCRIMEERVYMEDAPLLRGAVFFVGMALWGAHRLPALKNTPTLVLPSFYKAMSCANEVVSYEIVLSITRLIKKYGKELQVVTWDILLGIIERLLQQIQTIGSAELKAIVYELLTTVEELYEQNGYHGSTEKFFSLVEKCADKRPDASVLTLISYRAQSIQPAKEGWIQSLHRLMEKFFRNETRSVIRIKVLHILSFVLSTNRQLYEDELIEMVVIPQLSGIAEDRDLAVRKQATQLLVDLAEGCNTHHFTSLLDIIERVSSRSLVCSGPLEVSERDPTAESPMEDVRTAILGLLEILQSKLYSLPASHASRVYELLISHLQLHYKNKYCSTIASTIRLKVFDFFLMMRADSLHRLGVPNKDGVMRFSPYCYCDIGEPEKRVGEKKPAGSTSPPAGSPAPPAAPPSSAASTRSAYLSYSPAFSVLLQCLKMETDWKVLKLVLDKLPWMLQYKVLLLTSPCNLDQLCSTLCCMVTDRLISERLKKTPEGFSRTDVQLAVIPVLTAITSYHNYLEQSRQRELVQCLETGLIYRCAKQCVVALTMCTVEMPDIMIKLLPALIVKLTHISATVAMASPMLEFLSTLVRLPHLYANFVAEQYVSVFAISLPYTNPSKFNQYIVSLAHHVIAMWFIRCRLPFRKDFVQYITKGLRSNALLPFDDSHEQSPFRARSTSLNERPKSLRAAKVAKAAAAVANSSSSPVKELRDLSAMDAFRSRSISVSEHAVRRMHTSTTTCSLGSADENAVTQADEGLKTVHLELTETCLDMMARYVFSNFSALPKRSPIAEFLLAGGRSMTWLVGNKLVTITTSGGVRTQALLGLDMADRLGGGGGELTRSDPSLHTRMTKEAPAKLESQSSQQHNRAARIRVRSMSGGHALRAGPAQSLSPLVSPSEGELTAPLSPSSGPALDSLGPSSSTSATPSVPPTLKDNPTLAEFAPMLTQGWAEIFIRRPSGNTSWLMCLENPPSPFSSELGNMPLQELSSVLMAMEGVKEPPTQTASAPASTAVPAPAPVSEPLTQTHSSVGGKANLIQRSNTDSVVVLEEGSGIATANTPPEWLGSAEFEPMLSDPIFIAADKFTKTPPETLSRSSSTSSQDDEKSTLEEVSEGAIPIDQPPFGPSTPGSQGPDLPFQTHTQSQGHGLNKSSSSPELQTLPEAFAKAAMESESAMVDTSRPRAPSDGMPQPQQPQFAKEKVEGNTGVDTNVAGGPSPAGEGQAVSSSQSGGAGMRLSFPPAPVQSAPISPSGGHRPRGHTISVSAPSSRRERRAERDAYHSRPGPSNTEKISGLSPSFVFLQLYHSPFFGNEANKPLLLPKTQVIDRAVKVLDQMPPYDTHKIGVVFVGAGQVNNEVAILSNEYGSNRYASFLTGLGKLIHLKDCDPDQIFLGGLDQYGDDGEFTYCWHDDIMQAIFHIATLMPNRESDKGCCNKKRHIGNDFVMVVYNDSGEEYKLGTIKGQFNFVEVIIKPLDYECNLVTLQCRKDLEGLVDTTVAKIVSDRNLPLLVRQMALHANMASLVHQYRANPSDAYASKWLARLRHIKRIRTRAQEAIQSRSTPGISLTQGQSKPLSQSSAPTNVEATGQRKRLVSTVDDFTDFV

Target ORF information:

RefSeq Version	XM_004539039.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra TSC complex subunit 2 (tsc2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004539039.3

ORF Insert Sequence:

ATGAATAAAC AACCAAGCAA GGAGAGTCTC AAGGACAAGG TGAAGGGGAT CTTTGGGCTC 
GGTACACAGC GTCCTCCGAA CAAACAGAGT GACACTAAAC CCTCCGAGTT TATCATTACG 
GCTGACATCA TCAAGGAGCT CCACCCAGAT TGCGGTCTGA GCAACCGTGT TCGCACTATG 
AACCACATCT GTGAGCTTGC CAAAATGAAA AAGTTTGAGG AGCATGCAGT GGAGGCTGTA 
TGGAAAAATG TGGAAGACAT GCTGACTCCA GAGCAGCCAC CAGAGGCCAG ACATGCTGTC 
CTGCAGCTGC TCAGGGCCAT TATACAGGGG CAGGGTGAGC GACTCGGCCC TCTGAGAGCC 
TACTTTTTCA AGGTAATCAG AGACTACCAG CCGTGCAATG AAGACCTTTC TGACAGACTG 
GAGGTGTTTA AGGCTTTAAC AGAGAATGGA AAGGATATCA CATACCTGGA AGAGGACATA 
GCACGCTTTG TCCTCCTGTG GATGGATATA GGCCTTACAT CTGACTTTCT CCATGTTCTT 
GTTAATCTGG TGAAATTCAA TAGCTGCTAC TTGGACCAAA ATGTTTCCAT CATGGTCCAG 
AAAATTTGCC TGCTGTGCAA CAGAACGACG TCTTCGACTG ATATTGAGGT GGCTCTGCAA 
GTGCTCGATG CAGTTGTGTG TTACAACTGC CTGCCCTCCG ACTCTTTAAC CGTTTTCATA 
ATCACACTCT GCCGTACGGT GAACGTCAAA GAGTTTTGCG AGTCCTGTTG GAAGTTAATG 
AGAAAGGTTT TGGGGACTCA CCTCGGCCAT AGTGCAATTT ACACCATGTG TCGCATTATG 
GAGGAAAGGG TGTATATGGA GGATGCACCG TTGTTAAGAG GAGCAGTTTT CTTTGTTGGA 
ATGGCGCTTT GGGGCGCACA CAGACTGCCT GCACTCAAAA ATACACCGAC GCTTGTTCTG 
CCATCTTTCT ACAAGGCCAT GTCATGTGCC AACGAGGTGG TCTCCTACGA AATTGTTCTT 
TCAATAACAA GACTCATCAA AAAGTACGGT AAAGAGCTAC AAGTGGTCAC ATGGGACATC 
CTACTCGGCA TCATAGAGCG ACTGTTGCAA CAGATTCAGA CAATAGGCAG TGCGGAACTA 
AAGGCCATTG TTTATGAACT GTTGACCACC GTGGAAGAGC TTTATGAACA AAACGGTTAC 
CACGGCTCCA CTGAGAAGTT TTTCAGCCTG GTGGAGAAAT GTGCTGATAA AAGACCAGAT 
GCATCAGTGC TGACACTTAT CTCCTACAGG GCTCAGTCTA TCCAGCCTGC TAAAGAAGGC 
TGGATTCAGA GCCTTCACCG GCTCATGGAG AAATTCTTCA GGAACGAGAC TCGCAGTGTG 
ATAAGAATCA AAGTGCTTCA TATCCTGTCC TTCGTTCTCA GTACCAACCG GCAGCTCTAT 
GAGGATGAGT TGATTGAAAT GGTGGTGATT CCCCAGCTCA GTGGGATTGC TGAAGACCGG 
GACTTGGCAG TTAGAAAACA GGCTACTCAG CTATTAGTAG ACCTCGCTGA GGGCTGCAAC 
ACGCACCACT TCACCAGCCT GCTGGACATC ATTGAACGGG TGTCTAGCCG CTCTCTGGTG 
TGTTCAGGGC CCCTAGAGGT TTCTGAGAGA GACCCCACAG CAGAGTCTCC TATGGAGGAC 
GTCAGGACTG CTATTCTGGG CCTGCTTGAA ATCCTGCAGA GCAAACTTTA CAGTCTGCCA 
GCCAGCCATG CGAGTCGTGT TTATGAGTTG CTTATCAGCC ACCTGCAGCT TCATTACAAG 
AACAAGTACT GTTCAACTAT CGCCTCCACT ATTAGACTAA AGGTGTTTGA CTTCTTCCTC 
ATGATGCGTG CAGACTCTCT TCACCGTCTT GGGGTCCCAA ACAAAGATGG TGTCATGAGA 
TTCAGCCCTT ATTGCTACTG TGATATAGGG GAACCAGAGA AACGTGTTGG TGAAAAGAAG 
CCAGCAGGTT CAACCTCTCC TCCTGCTGGC AGCCCTGCTC CTCCTGCTGC TCCTCCCAGT 
TCAGCAGCCT CAACACGCTC AGCCTACCTT TCCTATTCGC CTGCCTTCAG TGTCCTCCTG 
CAGTGCCTCA AGATGGAGAC GGATTGGAAG GTGCTGAAGC TGGTTCTTGA CAAGCTGCCA 
TGGATGCTGC AGTACAAAGT GTTGCTGCTC ACATCACCAT GCAACTTAGA TCAGCTGTGT 
TCTACACTCT GCTGCATGGT GACAGATCGT CTCATCTCAG AGCGATTGAA AAAGACTCCA 
GAGGGGTTTT CTCGCACAGA TGTTCAGCTG GCAGTGATTC CCGTTCTTAC AGCAATTACC 
TCCTACCACA ACTACTTGGA GCAGTCAAGA CAGAGAGAAC TGGTTCAGTG CCTCGAGACT 
GGGCTCATTT ACCGCTGTGC CAAGCAATGT GTGGTGGCCC TTACAATGTG CACGGTGGAG 
ATGCCTGATA TCATGATCAA GCTTCTTCCT GCTCTTATCG TCAAGCTCAC TCACATCTCT 
GCTACTGTTG CCATGGCATC TCCTATGCTC GAGTTTCTCT CCACCCTCGT GCGCCTTCCC 
CATCTGTATG CCAACTTTGT AGCTGAGCAG TATGTCAGCG TGTTTGCCAT CTCACTACCT 
TACACTAACC CCTCAAAGTT CAACCAGTAC ATTGTGTCCC TGGCCCACCA TGTGATTGCC 
ATGTGGTTCA TACGCTGCAG ACTACCCTTC CGCAAGGACT TTGTTCAGTA TATCACAAAG 
GGTTTGCGTT CCAACGCCCT TCTGCCATTC GATGACAGTC ATGAGCAGAG TCCGTTTCGT 
GCCCGAAGCA CCAGCCTCAA TGAGAGACCC AAGAGCTTGC GGGCAGCAAA AGTGGCAAAG 
GCAGCAGCAG CGGTAGCCAA TAGCAGCAGC TCTCCAGTTA AAGAGCTGAG GGACCTTTCA 
GCCATGGACG CTTTCCGCTC CCGCAGCATC AGCGTCTCCG AACATGCGGT CCGCAGGATG 
CACACCTCAA CCACCACATG CAGTCTGGGC TCTGCCGATG AAAATGCAGT AACTCAGGCC 
GACGAGGGGC TGAAAACTGT CCACCTAGAG CTCACAGAGA CTTGTCTTGA CATGATGGCC 
CGATATGTAT TTTCCAACTT CTCTGCACTT CCCAAGAGGT CTCCCATCGC AGAGTTCCTG 
TTAGCAGGAG GCCGCAGTAT GACCTGGTTG GTGGGCAACA AGTTGGTCAC CATAACAACC 
AGCGGAGGGG TCAGAACACA AGCGCTGCTG GGCCTGGACA TGGCTGATCG CCTGGGAGGA 
GGAGGAGGGG AATTGACCAG GTCAGACCCA TCACTACACA CCCGAATGAC CAAAGAGGCT 
CCAGCCAAAT TGGAGTCACA GTCAAGCCAG CAGCACAACA GAGCTGCACG CATACGAGTT 
CGCTCCATGT CAGGTGGTCA TGCTCTACGT GCCGGTCCAG CTCAGAGTCT CAGTCCTCTG 
GTGTCTCCCT CTGAGGGTGA GTTGACTGCT CCACTCTCTC CCTCCTCTGG TCCTGCTCTG 
GATAGTTTAG GTCCATCCTC TTCCACCTCT GCCACTCCAT CTGTGCCTCC TACTCTGAAA 
GACAACCCCA CCTTGGCTGA ATTTGCCCCA ATGCTCACAC AAGGCTGGGC AGAGATCTTC 
ATACGCAGAC CGTCAGGTAA CACAAGCTGG CTAATGTGTT TGGAGAACCC ACCTAGCCCT 
TTCTCATCTG AGCTTGGCAA CATGCCGCTG CAAGAGCTCT CCAGTGTTCT GATGGCAATG 
GAGGGAGTGA AGGAACCTCC CACTCAGACA GCCAGTGCTC CTGCTAGCAC AGCTGTCCCT 
GCTCCAGCTC CTGTCTCTGA GCCCCTAACC CAAACACACA GCAGTGTTGG AGGAAAGGCA 
AACCTGATTC AGCGCTCCAA CACAGACTCT GTCGTGGTGC TGGAGGAGGG ATCAGGAATT 
GCTACAGCAA ACACGCCGCC TGAATGGCTG GGGAGTGCAG AGTTTGAGCC AATGTTGTCT 
GATCCAATCT TCATAGCTGC TGATAAGTTT ACAAAGACTC CTCCTGAAAC ACTCAGCAGG 
TCCTCCTCCA CCTCAAGTCA GGATGATGAA AAGTCAACCC TGGAAGAAGT AAGTGAGGGA 
GCAATTCCAA TCGATCAGCC CCCTTTTGGA CCATCCACTC CAGGAAGCCA GGGCCCTGAC 
CTTCCCTTCC AGACACACAC TCAGTCCCAG GGTCATGGAC TCAACAAGTC CAGCTCCTCT 
CCTGAGCTCC AGACATTACC TGAAGCTTTT GCCAAGGCTG CTATGGAGTC AGAAAGTGCG 
ATGGTTGACA CGTCACGGCC TAGAGCACCC TCAGACGGCA TGCCTCAACC CCAGCAGCCC 
CAGTTTGCGA AAGAAAAGGT AGAGGGAAAC ACTGGAGTGG ACACTAATGT AGCGGGAGGT 
CCAAGCCCAG CAGGTGAAGG CCAAGCTGTA TCGTCATCTC AGAGTGGAGG AGCGGGGATG 
AGGTTGTCTT TTCCACCAGC ACCAGTACAA TCTGCACCCA TCTCCCCCAG TGGAGGCCAC 
CGGCCCAGAG GTCACACAAT CTCAGTCTCA GCCCCCTCCT CCAGGAGGGA GAGGCGGGCG 
GAGAGGGACG CTTACCACAG TCGACCTGGT CCCAGCAACA CTGAGAAAAT CTCTGGACTG 
AGCCCCAGCT TTGTGTTTCT GCAGCTCTAC CACTCTCCTT TCTTTGGGAA TGAAGCCAAC 
AAGCCACTTC TGCTGCCCAA AACTCAGGTG ATTGACCGTG CAGTAAAGGT TCTGGACCAA 
ATGCCCCCTT ATGACACCCA TAAAATTGGG GTGGTGTTTG TAGGAGCTGG TCAGGTTAAC 
AATGAAGTCG CCATCTTGTC GAACGAGTAC GGTTCGAACC GCTACGCCTC TTTCCTCACA 
GGTCTTGGCA AATTAATCCA TCTGAAAGAC TGCGATCCAG ACCAGATTTT TCTTGGAGGG 
CTTGACCAGT ATGGAGATGA TGGAGAGTTC ACCTACTGCT GGCATGACGA CATCATGCAG 
GCTATCTTTC ACATAGCCAC ACTGATGCCA AACAGGGAGA GCGACAAGGG TTGCTGCAAC 
AAAAAGCGAC ACATTGGCAA CGATTTTGTC ATGGTGGTGT ACAATGACTC TGGAGAGGAG 
TACAAACTGG GCACAATTAA GGGTCAGTTT AATTTTGTCG AAGTCATTAT AAAACCACTG 
GATTATGAAT GTAACCTCGT TACCCTCCAG TGCCGCAAAG ACCTCGAGGG ATTAGTAGAC 
ACAACGGTGG CAAAAATTGT TTCAGATCGA AACCTTCCCC TGTTAGTCAG GCAGATGGCT 
CTGCATGCAA ATATGGCCTC ATTGGTGCAC CAGTACAGAG CTAACCCCTC TGACGCATAC 
GCCTCTAAGT GGCTAGCAAG GTTACGACAC ATCAAGAGGA TCAGGACCAG GGCTCAAGAA 
GCCATCCAGT CTCGCTCAAC TCCCGGGATT TCACTGACCC AAGGGCAGAG CAAACCTTTG 
TCACAGAGCA GTGCTCCAAC AAATGTGGAA GCCACCGGAC AACGAAAAAG ACTGGTGTCC 
ACTGTAGACG ATTTCACAGA TTTTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tsc2 ( XM_004539040.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004539097.1 Maylandia zebra tuberous sclerosis 2 (tsc2), transcript variant X5, mRNA.

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CloneID	OMa18057
Clone ID Related Accession (Same CDS sequence)	XM_004539040.1 , XM_004539040.2
Accession Version	XM_004539040.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-10-15
Organism	Maylandia zebra(Zebra mbuna)
Product	tuberin isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014446893.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAAAC AACCAAGCAA GGAGAGTCTC AAGGACAAGG TGAAGGGGAT CTTTGGGCTC 
GGTACACAGC GTCCTCCGAA CAAACAGAGT GACACTAAAC CCTCCGAGTT TATCATTACG 
GCTGACATCA TCAAGGAGCT CCACCCAGAT TGCGGTCTGA GCAACCGTGT TCGCACTATG 
AACCACATCT GTGAGCTTGC CAAAATGAAA AAGTTTGAGG AGCATGCAGT GGAGGCTGTA 
TGGAAAAATG TGGAAGACAT GCTGACTCCA GAGCAGCCAC CAGAGGCCAG ACATGCTGTC 
CTGCAGCTGC TCAGGGCCAT TATACAGGGG CAGGGTGAGC GACTCGGCCC TCTGAGAGCC 
TACTTTTTCA AGGTAATCAG AGACTACCAG CCGTGCAATG AAGACCTTTC TGACAGACTG 
GAGGTGTTTA AGGCTTTAAC AGAGAATGGA AAGGATATCA CATACCTGGA AGAGGACATA 
GCACGCTTTG TCCTCCTGTG GATGGATATA GGCCTTACAT CTGACTTTCT CCATGTTCTT 
GTTAATCTGG TGAAATTCAA TAGCTGCTAC TTGGACCAAA ATGTTTCCAT CATGGTCCAG 
AAAATTTGCC TGCTGTGCAA CAGAACGACG TCTTCGACTG ATATTGAGGT GGCTCTGCAA 
GTGCTCGATG CAGTTGTGTG TTACAACTGC CTGCCCTCCG ACTCTTTAAC CGTTTTCATA 
ATCACACTCT GCCGTACGGT GAACGTCAAA GAGTTTTGCG AGTCCTGTTG GAAGTTAATG 
AGAAAGGTTT TGGGGACTCA CCTCGGCCAT AGTGCAATTT ACACCATGTG TCGCATTATG 
GAGGAAAGGG TGTATATGGA GGATGCACCG TTGTTAAGAG GAGCAGTTTT CTTTGTTGGA 
ATGGCGCTTT GGGGCGCACA CAGACTGCCT GCACTCAAAA ATACACCGAC GCTTGTTCTG 
CCATCTTTCT ACAAGGCCAT GTCATGTGCC AACGAGGTGG TCTCCTACGA AATTGTTCTT 
TCAATAACAA GACTCATCAA AAAGTACGGT AAAGAGCTAC AAGTGGTCAC ATGGGACATC 
CTACTCGGCA TCATAGAGCG ACTGTTGCAA CAGATTCAGA CAATAGGCAG TGCGGAACTA 
AAGGCCATTG TTTATGAACT GTTGACCACC GTGGAAGAGC TTTATGAACA AAACGGTTAC 
CACGGCTCCA CTGAGAAGTT TTTCAGCCTG GTGGAGAAAT GTGCTGATAA AAGACCAGAT 
GCATCAGTGC TGACACTTAT CTCCTACAGG GCTCAGTCTA TCCAGCCTGC TAAAGAAGGC 
TGGATTCAGA GCCTTCACCG GCTCATGGAG AAATTCTTCA GGAACGAGAC TCGCAGTGTG 
ATAAGAATCA AAGTGCTTCA TATCCTGTCC TTCGTTCTCA GTACCAACCG GCAGCTCTAT 
GAGGATGAGT TGATTGAAAT GGTGGTGATT CCCCAGCTCA GTGGGATTGC TGAAGACCGG 
GACTTGGCAG TTAGAAAACA GGCTACTCAG CTATTAGTAG ACCTCGCTGA GGGCTGCAAC 
ACGCACCACT TCACCAGCCT GCTGGACATC ATTGAACGGG TGTCTAGCCG CTCTCTGGTG 
TGTTCAGGGC CCCTAGAGGT TTCTGAGAGA GACCCCACAG CAGAGTCTCC TATGGAGGAC 
GTCAGGACTG CTATTCTGGG CCTGCTTGAA ATCCTGCAGA GCAAACTTTA CAGTCTGCCA 
GCCAGCCATG CGAGTCGTGT TTATGAGTTG CTTATCAGCC ACCTGCAGCT TCATTACAAG 
AACAAGTACT GTTCAACTAT CGCCTCCACT ATTAGACTAA AGGTGTTTGA CTTCTTCCTC 
ATGATGCGTG CAGACTCTCT TCACCGTCTT GGGGTCCCAA ACAAAGATGG TGTCATGAGA 
TTCAGCCCTT ATTGCTACTG TGATATAGGG GAACCAGAGA AACGTGTTGG TGAAAAGAAG 
CCAGCAGGTT CAACCTCTCC TCCTGCTGGC AGCCCTGCTC CTCCTGCTGC TCCTCCCAGT 
TCAGCAGCCT CAACACGCTC AGCCTACCTT TCCTATTCGC CTGCCTTCAG TGTCCTCCTG 
CAGTGCCTCA AGATGGAGAC GGATTGGAAG GTGCTGAAGC TGGTTCTTGA CAAGCTGCCA 
TGGATGCTGC AGTACAAAGT GTTGCTGCTC ACATCACCAT GCAACTTAGA TCAGCTGTGT 
TCTACACTCT GCTGCATGGT GACAGATCGT CTCATCTCAG AGCGATTGAA AAAGACTCCA 
GAGGGGTTTT CTCGCACAGA TGTTCAGCTG GCAGTGATTC CCGTTCTTAC AGCAATTACC 
TCCTACCACA ACTACTTGGA GCAGTCAAGA CAGAGAGAAC TGGTTCAGTG CCTCGAGACT 
GGGCTCATTT ACCGCTGTGC CAAGCAATGT GTGGTGGCCC TTACAATGTG CACGGTGGAG 
ATGCCTGATA TCATGATCAA GCTTCTTCCT GCTCTTATCG TCAAGCTCAC TCACATCTCT 
GCTACTGTTG CCATGGCATC TCCTATGCTC GAGTTTCTCT CCACCCTCGT GCGCCTTCCC 
CATCTGTATG CCAACTTTGT AGCTGAGCAG TATGTCAGCG TGTTTGCCAT CTCACTACCT 
TACACTAACC CCTCAAAGTT CAACCAGTAC ATTGTGTCCC TGGCCCACCA TGTGATTGCC 
ATGTGGTTCA TACGCTGCAG ACTACCCTTC CGCAAGGACT TTGTTCAGTA TATCACAAAG 
GGTTTGCGTT CCAACGCCCT TCTGCCATTC GATGACAGTC ATGAGCAGAG TCCGTTTCGT 
GCCCGAAGCA CCAGCCTCAA TGAGAGACCC AAGAGGATGC ACACCTCAAC CACCACATGC 
AGTCTGGGCT CTGCCGATGA AAATGCAGTA ACTCAGGCCG ACGAGGGGCT GAAAACTGTC 
CACCTAGAGC TCACAGAGAC TTGTCTTGAC ATGATGGCCC GATATGTATT TTCCAACTTC 
TCTGCACTTC CCAAGAGGTC TCCCATCGCA GAGTTCCTGT TAGCAGGAGG CCGCAGTATG 
ACCTGGTTGG TGGGCAACAA GTTGGTCACC ATAACAACCA GCGGAGGGGT CAGAACACAA 
GCGCTGCTGG GCCTGGACAT GGCTGATCGC CTGGGAGGAG GAGGAGGGGA ATTGACCAGG 
TCAGACCCAT CACTACACAC CCGAATGACC AAAGAGGCTC CAGCCAAATT GGAGTCACAG 
TCAAGCCAGC AGCACAACAG AGCTGCACGC ATACGAGTTC GCTCCATGTC AGGTGGTCAT 
GCTCTACGTG CCGGTCCAGC TCAGAGTCTC AGTCCTCTGG TGTCTCCCTC TGAGGGTGAG 
TTGACTGCTC CACTCTCTCC CTCCTCTGGT CCTGCTCTGG ATAGTTTAGG TCCATCCTCT 
TCCACCTCTG CCACTCCATC TGTGCCTCCT ACTCTGAAAG ACAACCCCAC CTTGGCTGAA 
TTTGCCCCAA TGCTCACACA AGGCTGGGCA GAGATCTTCA TACGCAGACC GTCAGGTAAC 
ACAAGCTGGC TAATGTGTTT GGAGAACCCA CCTAGCCCTT TCTCATCTGA GCTTGGCAAC 
ATGCCGCTGC AAGAGCTCTC CAGTGTTCTG ATGGCAATGG AGGGAGTGAA GGAACCTCCC 
ACTCAGACAG CCAGTGCTCC TGCTAGCACA GCTGTCCCTG CTCCAGCTCC TGTCTCTGAG 
CCCCTAACCC AAACACACAG CAGTGTTGGA GGAAAGGCAA ACCTGATTCA GCGCTCCAAC 
ACAGTGGGTG GCTCTCTCTG CTCTCTGGGT ATGGGCACTG CCCCCGCAGG CCCACCAGCC 
CCTGGCAGGT TGCACAGGAG CATTTCCTGG GCAGACTCTG TCGTGGTGCT GGAGGAGGGA 
TCAGGAATTG CTACAGCAAA CACGCCGCCT GAATGGCTGG GGAGTGCAGA GTTTGAGCCA 
ATGTTGTCTG ATCCAATCTT CATAGCTGCT GATAAGTTTA CAAAGACTCC TCCTGAAACA 
CTCAGCAGGT CCTCCTCCAC CTCAAGTCAG GATGATGAAA AGTCAACCCT GGAAGAAGTA 
AGTGAGGGAG CAATTCCAAT CGATCAGCCC CCTTTTGGAC CATCCACTCC AGGAAGCCAG 
GGCCCTGACC TTCCCTTCCA GACACACACT CAGTCCCAGG GTCATGGACT CAACAAGTCC 
AGCTCCTCTC CTGAGCTCCA GACATTACCT GAAGCTTTTG CCAAGGCTGC TATGGAGTCA 
GAAAGTGCGA TGGTTGACAC GTCACGGCCT AGAGCACCCT CAGACGGCAT GCCTCAACCC 
CAGCAGCCCC AGTTTGCGAA AGAAAAGGTA GAGGGAAACA CTGGAGTGGA CACTAATGTA 
GCGGGAGGTC CAAGCCCAGC AGGTGAAGGC CAAGCTGTAT CGTCATCTCA GAGTGGAGGA 
GCGGGGATGA GGTTGTCTTT TCCACCAGCA CCAGTACAAT CTGCACCCAT CTCCCCCAGT 
GGAGGCCACC GGCCCAGAGG TCACACAATC TCAGTCTCAG CCCCCTCCTC CAGGAGGGAG 
AGGCGGGCGG AGAGGGACGC TTACCACAGT CGACCTGGTC CCAGCAACAC TGAGAAAATC 
TCTGGACTGA GCCCCAGCTT TGTGTTTCTG CAGCTCTACC ACTCTCCTTT CTTTGGGAAT 
GAAGCCAACA AGCCACTTCT GCTGCCCAAA ACTCAGGTGA TTGACCGTGC AGTAAAGGTT 
CTGGACCAAA TGCCCCCTTA TGACACCCAT AAAATTGGGG TGGTGTTTGT AGGAGCTGGT 
CAGGTTAACA ATGAAGTCGC CATCTTGTCG AACGAGTACG GTTCGAACCG CTACGCCTCT 
TTCCTCACAG GTCTTGGCAA ATTAATCCAT CTGAAAGACT GCGATCCAGA CCAGATTTTT 
CTTGGAGGGC TTGACCAGTA TGGAGATGAT GGAGAGTTCA CCTACTGCTG GCATGACGAC 
ATCATGCAGG CTATCTTTCA CATAGCCACA CTGATGCCAA ACAGGGAGAG CGACAAGGGT 
TGCTGCAACA AAAAGCGACA CATTGGCAAC GATTTTGTCA TGGTGGTGTA CAATGACTCT 
GGAGAGGAGT ACAAACTGGG CACAATTAAG GGTCAGTTTA ATTTTGTCGA AGTCATTATA 
AAACCACTGG ATTATGAATG TAACCTCGTT ACCCTCCAGT GCCGCAAAGA CCTCGAGGGA 
TTAGTAGACA CAACGGTGGC AAAAATTGTT TCAGATCGAA ACCTTCCCCT GTTAGTCAGG 
CAGATGGCTC TGCATGCAAA TATGGCCTCA TTGGTGCACC AGTACAGAGC TAACCCCTCT 
GACGCATACG CCTCTAAGTG GCTAGCAAGG TTACGACACA TCAAGAGGAT CAGGACCAGG 
GCTCAAGAAG CCATCCAGTC TCGCTCAACT CCCGGGATTT CACTGACCCA AGGGCAGAGC 
AAACCTTTGT CACAGAGCAG TGCTCCAACA AATGTGGAAG CCACCGGACA ACGAAAAAGA 
CTGGTGTCCA CTGTAGACGA TTTCACAGAT TTTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004539097.1
CDS	196..5754
Translation	MNKQPSKESLKDKVKGIFGLGTQRPPNKQSDTKPSEFIITADIIKELHPDCGLSNRVRTMNHICELAKMKKFEEHAVEAVWKNVEDMLTPEQPPEARHAVLQLLRAIIQGQGERLGPLRAYFFKVIRDYQPCNEDLSDRLEVFKALTENGKDITYLEEDIARFVLLWMDIGLTSDFLHVLVNLVKFNSCYLDQNVSIMVQKICLLCNRTTSSTDIEVALQVLDAVVCYNCLPSDSLTVFIITLCRTVNVKEFCESCWKLMRKVLGTHLGHSAIYTMCRIMEERVYMEDAPLLRGAVFFVGMALWGAHRLPALKNTPTLVLPSFYKAMSCANEVVSYEIVLSITRLIKKYGKELQVVTWDILLGIIERLLQQIQTIGSAELKAIVYELLTTVEELYEQNGYHGSTEKFFSLVEKCADKRPDASVLTLISYRAQSIQPAKEGWIQSLHRLMEKFFRNETRSVIRIKVLHILSFVLSTNRQLYEDELIEMVVIPQLSGIAEDRDLAVRKQATQLLVDLAEGCNTHHFTSLLDIIERVSSRSLVCSGPLEVSERDPTAESPMEDVRTAILGLLEILQSKLYSLPASHASRVYELLISHLQLHYKNKYCSTIASTIRLKVFDFFLMMRADSLHRLGVPNKDGVMRFSPYCYCDIGEPEKRVGEKKPAGSTSPPAGSPAPPAAPPSSAASTRSAYLSYSPAFSVLLQCLKMETDWKVLKLVLDKLPWMLQYKVLLLTSPCNLDQLCSTLCCMVTDRLISERLKKTPEGFSRTDVQLAVIPVLTAITSYHNYLEQSRQRELVQCLETGLIYRCAKQCVVALTMCTVEMPDIMIKLLPALIVKLTHISATVAMASPMLEFLSTLVRLPHLYANFVAEQYVSVFAISLPYTNPSKFNQYIVSLAHHVIAMWFIRCRLPFRKDFVQYITKGLRSNALLPFDDSHEQSPFRARSTSLNERPKRMHTSTTTCSLGSADENAVTQADEGLKTVHLELTETCLDMMARYVFSNFSALPKRSPIAEFLLAGGRSMTWLVGNKLVTITTSGGVRTQALLGLDMADRLGGGGGELTRSDPSLHTRMTKEAPAKLESQSSQQHNRAARIRVRSMSGGHALRAGPAQSLSPLVSPSEGELTAPLSPSSGPALDSLGPSSSTSATPSVPPTLKDNPTLAEFAPMLTQGWAEIFIRRPSGNTSWLMCLENPPSPFSSELGNMPLQELSSVLMAMEGVKEPPTQTASAPASTAVPAPAPVSEPLTQTHSSVGGKANLIQRSNTVGGSLCSLGMGTAPAGPPAPGRLHRSISWADSVVVLEEGSGIATANTPPEWLGSAEFEPMLSDPIFIAADKFTKTPPETLSRSSSTSSQDDEKSTLEEVSEGAIPIDQPPFGPSTPGSQGPDLPFQTHTQSQGHGLNKSSSSPELQTLPEAFAKAAMESESAMVDTSRPRAPSDGMPQPQQPQFAKEKVEGNTGVDTNVAGGPSPAGEGQAVSSSQSGGAGMRLSFPPAPVQSAPISPSGGHRPRGHTISVSAPSSRRERRAERDAYHSRPGPSNTEKISGLSPSFVFLQLYHSPFFGNEANKPLLLPKTQVIDRAVKVLDQMPPYDTHKIGVVFVGAGQVNNEVAILSNEYGSNRYASFLTGLGKLIHLKDCDPDQIFLGGLDQYGDDGEFTYCWHDDIMQAIFHIATLMPNRESDKGCCNKKRHIGNDFVMVVYNDSGEEYKLGTIKGQFNFVEVIIKPLDYECNLVTLQCRKDLEGLVDTTVAKIVSDRNLPLLVRQMALHANMASLVHQYRANPSDAYASKWLARLRHIKRIRTRAQEAIQSRSTPGISLTQGQSKPLSQSSAPTNVEATGQRKRLVSTVDDFTDFV

Target ORF information:

RefSeq Version	XM_004539040.1
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra tuberous sclerosis 2 (tsc2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004539040.1

ORF Insert Sequence:

ATGAATAAAC AACCAAGCAA GGAGAGTCTC AAGGACAAGG TGAAGGGGAT CTTTGGGCTC 
GGTACACAGC GTCCTCCGAA CAAACAGAGT GACACTAAAC CCTCCGAGTT TATCATTACG 
GCTGACATCA TCAAGGAGCT CCACCCAGAT TGCGGTCTGA GCAACCGTGT TCGCACTATG 
AACCACATCT GTGAGCTTGC CAAAATGAAA AAGTTTGAGG AGCATGCAGT GGAGGCTGTA 
TGGAAAAATG TGGAAGACAT GCTGACTCCA GAGCAGCCAC CAGAGGCCAG ACATGCTGTC 
CTGCAGCTGC TCAGGGCCAT TATACAGGGG CAGGGTGAGC GACTCGGCCC TCTGAGAGCC 
TACTTTTTCA AGGTAATCAG AGACTACCAG CCGTGCAATG AAGACCTTTC TGACAGACTG 
GAGGTGTTTA AGGCTTTAAC AGAGAATGGA AAGGATATCA CATACCTGGA AGAGGACATA 
GCACGCTTTG TCCTCCTGTG GATGGATATA GGCCTTACAT CTGACTTTCT CCATGTTCTT 
GTTAATCTGG TGAAATTCAA TAGCTGCTAC TTGGACCAAA ATGTTTCCAT CATGGTCCAG 
AAAATTTGCC TGCTGTGCAA CAGAACGACG TCTTCGACTG ATATTGAGGT GGCTCTGCAA 
GTGCTCGATG CAGTTGTGTG TTACAACTGC CTGCCCTCCG ACTCTTTAAC CGTTTTCATA 
ATCACACTCT GCCGTACGGT GAACGTCAAA GAGTTTTGCG AGTCCTGTTG GAAGTTAATG 
AGAAAGGTTT TGGGGACTCA CCTCGGCCAT AGTGCAATTT ACACCATGTG TCGCATTATG 
GAGGAAAGGG TGTATATGGA GGATGCACCG TTGTTAAGAG GAGCAGTTTT CTTTGTTGGA 
ATGGCGCTTT GGGGCGCACA CAGACTGCCT GCACTCAAAA ATACACCGAC GCTTGTTCTG 
CCATCTTTCT ACAAGGCCAT GTCATGTGCC AACGAGGTGG TCTCCTACGA AATTGTTCTT 
TCAATAACAA GACTCATCAA AAAGTACGGT AAAGAGCTAC AAGTGGTCAC ATGGGACATC 
CTACTCGGCA TCATAGAGCG ACTGTTGCAA CAGATTCAGA CAATAGGCAG TGCGGAACTA 
AAGGCCATTG TTTATGAACT GTTGACCACC GTGGAAGAGC TTTATGAACA AAACGGTTAC 
CACGGCTCCA CTGAGAAGTT TTTCAGCCTG GTGGAGAAAT GTGCTGATAA AAGACCAGAT 
GCATCAGTGC TGACACTTAT CTCCTACAGG GCTCAGTCTA TCCAGCCTGC TAAAGAAGGC 
TGGATTCAGA GCCTTCACCG GCTCATGGAG AAATTCTTCA GGAACGAGAC TCGCAGTGTG 
ATAAGAATCA AAGTGCTTCA TATCCTGTCC TTCGTTCTCA GTACCAACCG GCAGCTCTAT 
GAGGATGAGT TGATTGAAAT GGTGGTGATT CCCCAGCTCA GTGGGATTGC TGAAGACCGG 
GACTTGGCAG TTAGAAAACA GGCTACTCAG CTATTAGTAG ACCTCGCTGA GGGCTGCAAC 
ACGCACCACT TCACCAGCCT GCTGGACATC ATTGAACGGG TGTCTAGCCG CTCTCTGGTG 
TGTTCAGGGC CCCTAGAGGT TTCTGAGAGA GACCCCACAG CAGAGTCTCC TATGGAGGAC 
GTCAGGACTG CTATTCTGGG CCTGCTTGAA ATCCTGCAGA GCAAACTTTA CAGTCTGCCA 
GCCAGCCATG CGAGTCGTGT TTATGAGTTG CTTATCAGCC ACCTGCAGCT TCATTACAAG 
AACAAGTACT GTTCAACTAT CGCCTCCACT ATTAGACTAA AGGTGTTTGA CTTCTTCCTC 
ATGATGCGTG CAGACTCTCT TCACCGTCTT GGGGTCCCAA ACAAAGATGG TGTCATGAGA 
TTCAGCCCTT ATTGCTACTG TGATATAGGG GAACCAGAGA AACGTGTTGG TGAAAAGAAG 
CCAGCAGGTT CAACCTCTCC TCCTGCTGGC AGCCCTGCTC CTCCTGCTGC TCCTCCCAGT 
TCAGCAGCCT CAACACGCTC AGCCTACCTT TCCTATTCGC CTGCCTTCAG TGTCCTCCTG 
CAGTGCCTCA AGATGGAGAC GGATTGGAAG GTGCTGAAGC TGGTTCTTGA CAAGCTGCCA 
TGGATGCTGC AGTACAAAGT GTTGCTGCTC ACATCACCAT GCAACTTAGA TCAGCTGTGT 
TCTACACTCT GCTGCATGGT GACAGATCGT CTCATCTCAG AGCGATTGAA AAAGACTCCA 
GAGGGGTTTT CTCGCACAGA TGTTCAGCTG GCAGTGATTC CCGTTCTTAC AGCAATTACC 
TCCTACCACA ACTACTTGGA GCAGTCAAGA CAGAGAGAAC TGGTTCAGTG CCTCGAGACT 
GGGCTCATTT ACCGCTGTGC CAAGCAATGT GTGGTGGCCC TTACAATGTG CACGGTGGAG 
ATGCCTGATA TCATGATCAA GCTTCTTCCT GCTCTTATCG TCAAGCTCAC TCACATCTCT 
GCTACTGTTG CCATGGCATC TCCTATGCTC GAGTTTCTCT CCACCCTCGT GCGCCTTCCC 
CATCTGTATG CCAACTTTGT AGCTGAGCAG TATGTCAGCG TGTTTGCCAT CTCACTACCT 
TACACTAACC CCTCAAAGTT CAACCAGTAC ATTGTGTCCC TGGCCCACCA TGTGATTGCC 
ATGTGGTTCA TACGCTGCAG ACTACCCTTC CGCAAGGACT TTGTTCAGTA TATCACAAAG 
GGTTTGCGTT CCAACGCCCT TCTGCCATTC GATGACAGTC ATGAGCAGAG TCCGTTTCGT 
GCCCGAAGCA CCAGCCTCAA TGAGAGACCC AAGAGGATGC ACACCTCAAC CACCACATGC 
AGTCTGGGCT CTGCCGATGA AAATGCAGTA ACTCAGGCCG ACGAGGGGCT GAAAACTGTC 
CACCTAGAGC TCACAGAGAC TTGTCTTGAC ATGATGGCCC GATATGTATT TTCCAACTTC 
TCTGCACTTC CCAAGAGGTC TCCCATCGCA GAGTTCCTGT TAGCAGGAGG CCGCAGTATG 
ACCTGGTTGG TGGGCAACAA GTTGGTCACC ATAACAACCA GCGGAGGGGT CAGAACACAA 
GCGCTGCTGG GCCTGGACAT GGCTGATCGC CTGGGAGGAG GAGGAGGGGA ATTGACCAGG 
TCAGACCCAT CACTACACAC CCGAATGACC AAAGAGGCTC CAGCCAAATT GGAGTCACAG 
TCAAGCCAGC AGCACAACAG AGCTGCACGC ATACGAGTTC GCTCCATGTC AGGTGGTCAT 
GCTCTACGTG CCGGTCCAGC TCAGAGTCTC AGTCCTCTGG TGTCTCCCTC TGAGGGTGAG 
TTGACTGCTC CACTCTCTCC CTCCTCTGGT CCTGCTCTGG ATAGTTTAGG TCCATCCTCT 
TCCACCTCTG CCACTCCATC TGTGCCTCCT ACTCTGAAAG ACAACCCCAC CTTGGCTGAA 
TTTGCCCCAA TGCTCACACA AGGCTGGGCA GAGATCTTCA TACGCAGACC GTCAGGTAAC 
ACAAGCTGGC TAATGTGTTT GGAGAACCCA CCTAGCCCTT TCTCATCTGA GCTTGGCAAC 
ATGCCGCTGC AAGAGCTCTC CAGTGTTCTG ATGGCAATGG AGGGAGTGAA GGAACCTCCC 
ACTCAGACAG CCAGTGCTCC TGCTAGCACA GCTGTCCCTG CTCCAGCTCC TGTCTCTGAG 
CCCCTAACCC AAACACACAG CAGTGTTGGA GGAAAGGCAA ACCTGATTCA GCGCTCCAAC 
ACAGTGGGTG GCTCTCTCTG CTCTCTGGGT ATGGGCACTG CCCCCGCAGG CCCACCAGCC 
CCTGGCAGGT TGCACAGGAG CATTTCCTGG GCAGACTCTG TCGTGGTGCT GGAGGAGGGA 
TCAGGAATTG CTACAGCAAA CACGCCGCCT GAATGGCTGG GGAGTGCAGA GTTTGAGCCA 
ATGTTGTCTG ATCCAATCTT CATAGCTGCT GATAAGTTTA CAAAGACTCC TCCTGAAACA 
CTCAGCAGGT CCTCCTCCAC CTCAAGTCAG GATGATGAAA AGTCAACCCT GGAAGAAGTA 
AGTGAGGGAG CAATTCCAAT CGATCAGCCC CCTTTTGGAC CATCCACTCC AGGAAGCCAG 
GGCCCTGACC TTCCCTTCCA GACACACACT CAGTCCCAGG GTCATGGACT CAACAAGTCC 
AGCTCCTCTC CTGAGCTCCA GACATTACCT GAAGCTTTTG CCAAGGCTGC TATGGAGTCA 
GAAAGTGCGA TGGTTGACAC GTCACGGCCT AGAGCACCCT CAGACGGCAT GCCTCAACCC 
CAGCAGCCCC AGTTTGCGAA AGAAAAGGTA GAGGGAAACA CTGGAGTGGA CACTAATGTA 
GCGGGAGGTC CAAGCCCAGC AGGTGAAGGC CAAGCTGTAT CGTCATCTCA GAGTGGAGGA 
GCGGGGATGA GGTTGTCTTT TCCACCAGCA CCAGTACAAT CTGCACCCAT CTCCCCCAGT 
GGAGGCCACC GGCCCAGAGG TCACACAATC TCAGTCTCAG CCCCCTCCTC CAGGAGGGAG 
AGGCGGGCGG AGAGGGACGC TTACCACAGT CGACCTGGTC CCAGCAACAC TGAGAAAATC 
TCTGGACTGA GCCCCAGCTT TGTGTTTCTG CAGCTCTACC ACTCTCCTTT CTTTGGGAAT 
GAAGCCAACA AGCCACTTCT GCTGCCCAAA ACTCAGGTGA TTGACCGTGC AGTAAAGGTT 
CTGGACCAAA TGCCCCCTTA TGACACCCAT AAAATTGGGG TGGTGTTTGT AGGAGCTGGT 
CAGGTTAACA ATGAAGTCGC CATCTTGTCG AACGAGTACG GTTCGAACCG CTACGCCTCT 
TTCCTCACAG GTCTTGGCAA ATTAATCCAT CTGAAAGACT GCGATCCAGA CCAGATTTTT 
CTTGGAGGGC TTGACCAGTA TGGAGATGAT GGAGAGTTCA CCTACTGCTG GCATGACGAC 
ATCATGCAGG CTATCTTTCA CATAGCCACA CTGATGCCAA ACAGGGAGAG CGACAAGGGT 
TGCTGCAACA AAAAGCGACA CATTGGCAAC GATTTTGTCA TGGTGGTGTA CAATGACTCT 
GGAGAGGAGT ACAAACTGGG CACAATTAAG GGTCAGTTTA ATTTTGTCGA AGTCATTATA 
AAACCACTGG ATTATGAATG TAACCTCGTT ACCCTCCAGT GCCGCAAAGA CCTCGAGGGA 
TTAGTAGACA CAACGGTGGC AAAAATTGTT TCAGATCGAA ACCTTCCCCT GTTAGTCAGG 
CAGATGGCTC TGCATGCAAA TATGGCCTCA TTGGTGCACC AGTACAGAGC TAACCCCTCT 
GACGCATACG CCTCTAAGTG GCTAGCAAGG TTACGACACA TCAAGAGGAT CAGGACCAGG 
GCTCAAGAAG CCATCCAGTC TCGCTCAACT CCCGGGATTT CACTGACCCA AGGGCAGAGC 
AAACCTTTGT CACAGAGCAG TGCTCCAACA AATGTGGAAG CCACCGGACA ACGAAAAAGA 
CTGGTGTCCA CTGTAGACGA TTTCACAGAT TTTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tsc2 ( XM_004539040.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004539097.1 Maylandia zebra TSC complex subunit 2 (tsc2), transcript variant X5, mRNA.

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CloneID	OMa18057
Clone ID Related Accession (Same CDS sequence)	XM_004539040.1 , XM_004539040.2
Accession Version	XM_004539040.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-29
Organism	Maylandia zebra(zebra mbuna)
Product	tuberin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014446893.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 30, 2017 this sequence version replaced XM_004539040.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAAAC AACCAAGCAA GGAGAGTCTC AAGGACAAGG TGAAGGGGAT CTTTGGGCTC 
GGTACACAGC GTCCTCCGAA CAAACAGAGT GACACTAAAC CCTCCGAGTT TATCATTACG 
GCTGACATCA TCAAGGAGCT CCACCCAGAT TGCGGTCTGA GCAACCGTGT TCGCACTATG 
AACCACATCT GTGAGCTTGC CAAAATGAAA AAGTTTGAGG AGCATGCAGT GGAGGCTGTA 
TGGAAAAATG TGGAAGACAT GCTGACTCCA GAGCAGCCAC CAGAGGCCAG ACATGCTGTC 
CTGCAGCTGC TCAGGGCCAT TATACAGGGG CAGGGTGAGC GACTCGGCCC TCTGAGAGCC 
TACTTTTTCA AGGTAATCAG AGACTACCAG CCGTGCAATG AAGACCTTTC TGACAGACTG 
GAGGTGTTTA AGGCTTTAAC AGAGAATGGA AAGGATATCA CATACCTGGA AGAGGACATA 
GCACGCTTTG TCCTCCTGTG GATGGATATA GGCCTTACAT CTGACTTTCT CCATGTTCTT 
GTTAATCTGG TGAAATTCAA TAGCTGCTAC TTGGACCAAA ATGTTTCCAT CATGGTCCAG 
AAAATTTGCC TGCTGTGCAA CAGAACGACG TCTTCGACTG ATATTGAGGT GGCTCTGCAA 
GTGCTCGATG CAGTTGTGTG TTACAACTGC CTGCCCTCCG ACTCTTTAAC CGTTTTCATA 
ATCACACTCT GCCGTACGGT GAACGTCAAA GAGTTTTGCG AGTCCTGTTG GAAGTTAATG 
AGAAAGGTTT TGGGGACTCA CCTCGGCCAT AGTGCAATTT ACACCATGTG TCGCATTATG 
GAGGAAAGGG TGTATATGGA GGATGCACCG TTGTTAAGAG GAGCAGTTTT CTTTGTTGGA 
ATGGCGCTTT GGGGCGCACA CAGACTGCCT GCACTCAAAA ATACACCGAC GCTTGTTCTG 
CCATCTTTCT ACAAGGCCAT GTCATGTGCC AACGAGGTGG TCTCCTACGA AATTGTTCTT 
TCAATAACAA GACTCATCAA AAAGTACGGT AAAGAGCTAC AAGTGGTCAC ATGGGACATC 
CTACTCGGCA TCATAGAGCG ACTGTTGCAA CAGATTCAGA CAATAGGCAG TGCGGAACTA 
AAGGCCATTG TTTATGAACT GTTGACCACC GTGGAAGAGC TTTATGAACA AAACGGTTAC 
CACGGCTCCA CTGAGAAGTT TTTCAGCCTG GTGGAGAAAT GTGCTGATAA AAGACCAGAT 
GCATCAGTGC TGACACTTAT CTCCTACAGG GCTCAGTCTA TCCAGCCTGC TAAAGAAGGC 
TGGATTCAGA GCCTTCACCG GCTCATGGAG AAATTCTTCA GGAACGAGAC TCGCAGTGTG 
ATAAGAATCA AAGTGCTTCA TATCCTGTCC TTCGTTCTCA GTACCAACCG GCAGCTCTAT 
GAGGATGAGT TGATTGAAAT GGTGGTGATT CCCCAGCTCA GTGGGATTGC TGAAGACCGG 
GACTTGGCAG TTAGAAAACA GGCTACTCAG CTATTAGTAG ACCTCGCTGA GGGCTGCAAC 
ACGCACCACT TCACCAGCCT GCTGGACATC ATTGAACGGG TGTCTAGCCG CTCTCTGGTG 
TGTTCAGGGC CCCTAGAGGT TTCTGAGAGA GACCCCACAG CAGAGTCTCC TATGGAGGAC 
GTCAGGACTG CTATTCTGGG CCTGCTTGAA ATCCTGCAGA GCAAACTTTA CAGTCTGCCA 
GCCAGCCATG CGAGTCGTGT TTATGAGTTG CTTATCAGCC ACCTGCAGCT TCATTACAAG 
AACAAGTACT GTTCAACTAT CGCCTCCACT ATTAGACTAA AGGTGTTTGA CTTCTTCCTC 
ATGATGCGTG CAGACTCTCT TCACCGTCTT GGGGTCCCAA ACAAAGATGG TGTCATGAGA 
TTCAGCCCTT ATTGCTACTG TGATATAGGG GAACCAGAGA AACGTGTTGG TGAAAAGAAG 
CCAGCAGGTT CAACCTCTCC TCCTGCTGGC AGCCCTGCTC CTCCTGCTGC TCCTCCCAGT 
TCAGCAGCCT CAACACGCTC AGCCTACCTT TCCTATTCGC CTGCCTTCAG TGTCCTCCTG 
CAGTGCCTCA AGATGGAGAC GGATTGGAAG GTGCTGAAGC TGGTTCTTGA CAAGCTGCCA 
TGGATGCTGC AGTACAAAGT GTTGCTGCTC ACATCACCAT GCAACTTAGA TCAGCTGTGT 
TCTACACTCT GCTGCATGGT GACAGATCGT CTCATCTCAG AGCGATTGAA AAAGACTCCA 
GAGGGGTTTT CTCGCACAGA TGTTCAGCTG GCAGTGATTC CCGTTCTTAC AGCAATTACC 
TCCTACCACA ACTACTTGGA GCAGTCAAGA CAGAGAGAAC TGGTTCAGTG CCTCGAGACT 
GGGCTCATTT ACCGCTGTGC CAAGCAATGT GTGGTGGCCC TTACAATGTG CACGGTGGAG 
ATGCCTGATA TCATGATCAA GCTTCTTCCT GCTCTTATCG TCAAGCTCAC TCACATCTCT 
GCTACTGTTG CCATGGCATC TCCTATGCTC GAGTTTCTCT CCACCCTCGT GCGCCTTCCC 
CATCTGTATG CCAACTTTGT AGCTGAGCAG TATGTCAGCG TGTTTGCCAT CTCACTACCT 
TACACTAACC CCTCAAAGTT CAACCAGTAC ATTGTGTCCC TGGCCCACCA TGTGATTGCC 
ATGTGGTTCA TACGCTGCAG ACTACCCTTC CGCAAGGACT TTGTTCAGTA TATCACAAAG 
GGTTTGCGTT CCAACGCCCT TCTGCCATTC GATGACAGTC ATGAGCAGAG TCCGTTTCGT 
GCCCGAAGCA CCAGCCTCAA TGAGAGACCC AAGAGGATGC ACACCTCAAC CACCACATGC 
AGTCTGGGCT CTGCCGATGA AAATGCAGTA ACTCAGGCCG ACGAGGGGCT GAAAACTGTC 
CACCTAGAGC TCACAGAGAC TTGTCTTGAC ATGATGGCCC GATATGTATT TTCCAACTTC 
TCTGCACTTC CCAAGAGGTC TCCCATCGCA GAGTTCCTGT TAGCAGGAGG CCGCAGTATG 
ACCTGGTTGG TGGGCAACAA GTTGGTCACC ATAACAACCA GCGGAGGGGT CAGAACACAA 
GCGCTGCTGG GCCTGGACAT GGCTGATCGC CTGGGAGGAG GAGGAGGGGA ATTGACCAGG 
TCAGACCCAT CACTACACAC CCGAATGACC AAAGAGGCTC CAGCCAAATT GGAGTCACAG 
TCAAGCCAGC AGCACAACAG AGCTGCACGC ATACGAGTTC GCTCCATGTC AGGTGGTCAT 
GCTCTACGTG CCGGTCCAGC TCAGAGTCTC AGTCCTCTGG TGTCTCCCTC TGAGGGTGAG 
TTGACTGCTC CACTCTCTCC CTCCTCTGGT CCTGCTCTGG ATAGTTTAGG TCCATCCTCT 
TCCACCTCTG CCACTCCATC TGTGCCTCCT ACTCTGAAAG ACAACCCCAC CTTGGCTGAA 
TTTGCCCCAA TGCTCACACA AGGCTGGGCA GAGATCTTCA TACGCAGACC GTCAGGTAAC 
ACAAGCTGGC TAATGTGTTT GGAGAACCCA CCTAGCCCTT TCTCATCTGA GCTTGGCAAC 
ATGCCGCTGC AAGAGCTCTC CAGTGTTCTG ATGGCAATGG AGGGAGTGAA GGAACCTCCC 
ACTCAGACAG CCAGTGCTCC TGCTAGCACA GCTGTCCCTG CTCCAGCTCC TGTCTCTGAG 
CCCCTAACCC AAACACACAG CAGTGTTGGA GGAAAGGCAA ACCTGATTCA GCGCTCCAAC 
ACAGTGGGTG GCTCTCTCTG CTCTCTGGGT ATGGGCACTG CCCCCGCAGG CCCACCAGCC 
CCTGGCAGGT TGCACAGGAG CATTTCCTGG GCAGACTCTG TCGTGGTGCT GGAGGAGGGA 
TCAGGAATTG CTACAGCAAA CACGCCGCCT GAATGGCTGG GGAGTGCAGA GTTTGAGCCA 
ATGTTGTCTG ATCCAATCTT CATAGCTGCT GATAAGTTTA CAAAGACTCC TCCTGAAACA 
CTCAGCAGGT CCTCCTCCAC CTCAAGTCAG GATGATGAAA AGTCAACCCT GGAAGAAGTA 
AGTGAGGGAG CAATTCCAAT CGATCAGCCC CCTTTTGGAC CATCCACTCC AGGAAGCCAG 
GGCCCTGACC TTCCCTTCCA GACACACACT CAGTCCCAGG GTCATGGACT CAACAAGTCC 
AGCTCCTCTC CTGAGCTCCA GACATTACCT GAAGCTTTTG CCAAGGCTGC TATGGAGTCA 
GAAAGTGCGA TGGTTGACAC GTCACGGCCT AGAGCACCCT CAGACGGCAT GCCTCAACCC 
CAGCAGCCCC AGTTTGCGAA AGAAAAGGTA GAGGGAAACA CTGGAGTGGA CACTAATGTA 
GCGGGAGGTC CAAGCCCAGC AGGTGAAGGC CAAGCTGTAT CGTCATCTCA GAGTGGAGGA 
GCGGGGATGA GGTTGTCTTT TCCACCAGCA CCAGTACAAT CTGCACCCAT CTCCCCCAGT 
GGAGGCCACC GGCCCAGAGG TCACACAATC TCAGTCTCAG CCCCCTCCTC CAGGAGGGAG 
AGGCGGGCGG AGAGGGACGC TTACCACAGT CGACCTGGTC CCAGCAACAC TGAGAAAATC 
TCTGGACTGA GCCCCAGCTT TGTGTTTCTG CAGCTCTACC ACTCTCCTTT CTTTGGGAAT 
GAAGCCAACA AGCCACTTCT GCTGCCCAAA ACTCAGGTGA TTGACCGTGC AGTAAAGGTT 
CTGGACCAAA TGCCCCCTTA TGACACCCAT AAAATTGGGG TGGTGTTTGT AGGAGCTGGT 
CAGGTTAACA ATGAAGTCGC CATCTTGTCG AACGAGTACG GTTCGAACCG CTACGCCTCT 
TTCCTCACAG GTCTTGGCAA ATTAATCCAT CTGAAAGACT GCGATCCAGA CCAGATTTTT 
CTTGGAGGGC TTGACCAGTA TGGAGATGAT GGAGAGTTCA CCTACTGCTG GCATGACGAC 
ATCATGCAGG CTATCTTTCA CATAGCCACA CTGATGCCAA ACAGGGAGAG CGACAAGGGT 
TGCTGCAACA AAAAGCGACA CATTGGCAAC GATTTTGTCA TGGTGGTGTA CAATGACTCT 
GGAGAGGAGT ACAAACTGGG CACAATTAAG GGTCAGTTTA ATTTTGTCGA AGTCATTATA 
AAACCACTGG ATTATGAATG TAACCTCGTT ACCCTCCAGT GCCGCAAAGA CCTCGAGGGA 
TTAGTAGACA CAACGGTGGC AAAAATTGTT TCAGATCGAA ACCTTCCCCT GTTAGTCAGG 
CAGATGGCTC TGCATGCAAA TATGGCCTCA TTGGTGCACC AGTACAGAGC TAACCCCTCT 
GACGCATACG CCTCTAAGTG GCTAGCAAGG TTACGACACA TCAAGAGGAT CAGGACCAGG 
GCTCAAGAAG CCATCCAGTC TCGCTCAACT CCCGGGATTT CACTGACCCA AGGGCAGAGC 
AAACCTTTGT CACAGAGCAG TGCTCCAACA AATGTGGAAG CCACCGGACA ACGAAAAAGA 
CTGGTGTCCA CTGTAGACGA TTTCACAGAT TTTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004539097.1
CDS	198..5756
Translation	MNKQPSKESLKDKVKGIFGLGTQRPPNKQSDTKPSEFIITADIIKELHPDCGLSNRVRTMNHICELAKMKKFEEHAVEAVWKNVEDMLTPEQPPEARHAVLQLLRAIIQGQGERLGPLRAYFFKVIRDYQPCNEDLSDRLEVFKALTENGKDITYLEEDIARFVLLWMDIGLTSDFLHVLVNLVKFNSCYLDQNVSIMVQKICLLCNRTTSSTDIEVALQVLDAVVCYNCLPSDSLTVFIITLCRTVNVKEFCESCWKLMRKVLGTHLGHSAIYTMCRIMEERVYMEDAPLLRGAVFFVGMALWGAHRLPALKNTPTLVLPSFYKAMSCANEVVSYEIVLSITRLIKKYGKELQVVTWDILLGIIERLLQQIQTIGSAELKAIVYELLTTVEELYEQNGYHGSTEKFFSLVEKCADKRPDASVLTLISYRAQSIQPAKEGWIQSLHRLMEKFFRNETRSVIRIKVLHILSFVLSTNRQLYEDELIEMVVIPQLSGIAEDRDLAVRKQATQLLVDLAEGCNTHHFTSLLDIIERVSSRSLVCSGPLEVSERDPTAESPMEDVRTAILGLLEILQSKLYSLPASHASRVYELLISHLQLHYKNKYCSTIASTIRLKVFDFFLMMRADSLHRLGVPNKDGVMRFSPYCYCDIGEPEKRVGEKKPAGSTSPPAGSPAPPAAPPSSAASTRSAYLSYSPAFSVLLQCLKMETDWKVLKLVLDKLPWMLQYKVLLLTSPCNLDQLCSTLCCMVTDRLISERLKKTPEGFSRTDVQLAVIPVLTAITSYHNYLEQSRQRELVQCLETGLIYRCAKQCVVALTMCTVEMPDIMIKLLPALIVKLTHISATVAMASPMLEFLSTLVRLPHLYANFVAEQYVSVFAISLPYTNPSKFNQYIVSLAHHVIAMWFIRCRLPFRKDFVQYITKGLRSNALLPFDDSHEQSPFRARSTSLNERPKRMHTSTTTCSLGSADENAVTQADEGLKTVHLELTETCLDMMARYVFSNFSALPKRSPIAEFLLAGGRSMTWLVGNKLVTITTSGGVRTQALLGLDMADRLGGGGGELTRSDPSLHTRMTKEAPAKLESQSSQQHNRAARIRVRSMSGGHALRAGPAQSLSPLVSPSEGELTAPLSPSSGPALDSLGPSSSTSATPSVPPTLKDNPTLAEFAPMLTQGWAEIFIRRPSGNTSWLMCLENPPSPFSSELGNMPLQELSSVLMAMEGVKEPPTQTASAPASTAVPAPAPVSEPLTQTHSSVGGKANLIQRSNTVGGSLCSLGMGTAPAGPPAPGRLHRSISWADSVVVLEEGSGIATANTPPEWLGSAEFEPMLSDPIFIAADKFTKTPPETLSRSSSTSSQDDEKSTLEEVSEGAIPIDQPPFGPSTPGSQGPDLPFQTHTQSQGHGLNKSSSSPELQTLPEAFAKAAMESESAMVDTSRPRAPSDGMPQPQQPQFAKEKVEGNTGVDTNVAGGPSPAGEGQAVSSSQSGGAGMRLSFPPAPVQSAPISPSGGHRPRGHTISVSAPSSRRERRAERDAYHSRPGPSNTEKISGLSPSFVFLQLYHSPFFGNEANKPLLLPKTQVIDRAVKVLDQMPPYDTHKIGVVFVGAGQVNNEVAILSNEYGSNRYASFLTGLGKLIHLKDCDPDQIFLGGLDQYGDDGEFTYCWHDDIMQAIFHIATLMPNRESDKGCCNKKRHIGNDFVMVVYNDSGEEYKLGTIKGQFNFVEVIIKPLDYECNLVTLQCRKDLEGLVDTTVAKIVSDRNLPLLVRQMALHANMASLVHQYRANPSDAYASKWLARLRHIKRIRTRAQEAIQSRSTPGISLTQGQSKPLSQSSAPTNVEATGQRKRLVSTVDDFTDFV

Target ORF information:

RefSeq Version	XM_004539040.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra TSC complex subunit 2 (tsc2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004539040.2

ORF Insert Sequence:

ATGAATAAAC AACCAAGCAA GGAGAGTCTC AAGGACAAGG TGAAGGGGAT CTTTGGGCTC 
GGTACACAGC GTCCTCCGAA CAAACAGAGT GACACTAAAC CCTCCGAGTT TATCATTACG 
GCTGACATCA TCAAGGAGCT CCACCCAGAT TGCGGTCTGA GCAACCGTGT TCGCACTATG 
AACCACATCT GTGAGCTTGC CAAAATGAAA AAGTTTGAGG AGCATGCAGT GGAGGCTGTA 
TGGAAAAATG TGGAAGACAT GCTGACTCCA GAGCAGCCAC CAGAGGCCAG ACATGCTGTC 
CTGCAGCTGC TCAGGGCCAT TATACAGGGG CAGGGTGAGC GACTCGGCCC TCTGAGAGCC 
TACTTTTTCA AGGTAATCAG AGACTACCAG CCGTGCAATG AAGACCTTTC TGACAGACTG 
GAGGTGTTTA AGGCTTTAAC AGAGAATGGA AAGGATATCA CATACCTGGA AGAGGACATA 
GCACGCTTTG TCCTCCTGTG GATGGATATA GGCCTTACAT CTGACTTTCT CCATGTTCTT 
GTTAATCTGG TGAAATTCAA TAGCTGCTAC TTGGACCAAA ATGTTTCCAT CATGGTCCAG 
AAAATTTGCC TGCTGTGCAA CAGAACGACG TCTTCGACTG ATATTGAGGT GGCTCTGCAA 
GTGCTCGATG CAGTTGTGTG TTACAACTGC CTGCCCTCCG ACTCTTTAAC CGTTTTCATA 
ATCACACTCT GCCGTACGGT GAACGTCAAA GAGTTTTGCG AGTCCTGTTG GAAGTTAATG 
AGAAAGGTTT TGGGGACTCA CCTCGGCCAT AGTGCAATTT ACACCATGTG TCGCATTATG 
GAGGAAAGGG TGTATATGGA GGATGCACCG TTGTTAAGAG GAGCAGTTTT CTTTGTTGGA 
ATGGCGCTTT GGGGCGCACA CAGACTGCCT GCACTCAAAA ATACACCGAC GCTTGTTCTG 
CCATCTTTCT ACAAGGCCAT GTCATGTGCC AACGAGGTGG TCTCCTACGA AATTGTTCTT 
TCAATAACAA GACTCATCAA AAAGTACGGT AAAGAGCTAC AAGTGGTCAC ATGGGACATC 
CTACTCGGCA TCATAGAGCG ACTGTTGCAA CAGATTCAGA CAATAGGCAG TGCGGAACTA 
AAGGCCATTG TTTATGAACT GTTGACCACC GTGGAAGAGC TTTATGAACA AAACGGTTAC 
CACGGCTCCA CTGAGAAGTT TTTCAGCCTG GTGGAGAAAT GTGCTGATAA AAGACCAGAT 
GCATCAGTGC TGACACTTAT CTCCTACAGG GCTCAGTCTA TCCAGCCTGC TAAAGAAGGC 
TGGATTCAGA GCCTTCACCG GCTCATGGAG AAATTCTTCA GGAACGAGAC TCGCAGTGTG 
ATAAGAATCA AAGTGCTTCA TATCCTGTCC TTCGTTCTCA GTACCAACCG GCAGCTCTAT 
GAGGATGAGT TGATTGAAAT GGTGGTGATT CCCCAGCTCA GTGGGATTGC TGAAGACCGG 
GACTTGGCAG TTAGAAAACA GGCTACTCAG CTATTAGTAG ACCTCGCTGA GGGCTGCAAC 
ACGCACCACT TCACCAGCCT GCTGGACATC ATTGAACGGG TGTCTAGCCG CTCTCTGGTG 
TGTTCAGGGC CCCTAGAGGT TTCTGAGAGA GACCCCACAG CAGAGTCTCC TATGGAGGAC 
GTCAGGACTG CTATTCTGGG CCTGCTTGAA ATCCTGCAGA GCAAACTTTA CAGTCTGCCA 
GCCAGCCATG CGAGTCGTGT TTATGAGTTG CTTATCAGCC ACCTGCAGCT TCATTACAAG 
AACAAGTACT GTTCAACTAT CGCCTCCACT ATTAGACTAA AGGTGTTTGA CTTCTTCCTC 
ATGATGCGTG CAGACTCTCT TCACCGTCTT GGGGTCCCAA ACAAAGATGG TGTCATGAGA 
TTCAGCCCTT ATTGCTACTG TGATATAGGG GAACCAGAGA AACGTGTTGG TGAAAAGAAG 
CCAGCAGGTT CAACCTCTCC TCCTGCTGGC AGCCCTGCTC CTCCTGCTGC TCCTCCCAGT 
TCAGCAGCCT CAACACGCTC AGCCTACCTT TCCTATTCGC CTGCCTTCAG TGTCCTCCTG 
CAGTGCCTCA AGATGGAGAC GGATTGGAAG GTGCTGAAGC TGGTTCTTGA CAAGCTGCCA 
TGGATGCTGC AGTACAAAGT GTTGCTGCTC ACATCACCAT GCAACTTAGA TCAGCTGTGT 
TCTACACTCT GCTGCATGGT GACAGATCGT CTCATCTCAG AGCGATTGAA AAAGACTCCA 
GAGGGGTTTT CTCGCACAGA TGTTCAGCTG GCAGTGATTC CCGTTCTTAC AGCAATTACC 
TCCTACCACA ACTACTTGGA GCAGTCAAGA CAGAGAGAAC TGGTTCAGTG CCTCGAGACT 
GGGCTCATTT ACCGCTGTGC CAAGCAATGT GTGGTGGCCC TTACAATGTG CACGGTGGAG 
ATGCCTGATA TCATGATCAA GCTTCTTCCT GCTCTTATCG TCAAGCTCAC TCACATCTCT 
GCTACTGTTG CCATGGCATC TCCTATGCTC GAGTTTCTCT CCACCCTCGT GCGCCTTCCC 
CATCTGTATG CCAACTTTGT AGCTGAGCAG TATGTCAGCG TGTTTGCCAT CTCACTACCT 
TACACTAACC CCTCAAAGTT CAACCAGTAC ATTGTGTCCC TGGCCCACCA TGTGATTGCC 
ATGTGGTTCA TACGCTGCAG ACTACCCTTC CGCAAGGACT TTGTTCAGTA TATCACAAAG 
GGTTTGCGTT CCAACGCCCT TCTGCCATTC GATGACAGTC ATGAGCAGAG TCCGTTTCGT 
GCCCGAAGCA CCAGCCTCAA TGAGAGACCC AAGAGGATGC ACACCTCAAC CACCACATGC 
AGTCTGGGCT CTGCCGATGA AAATGCAGTA ACTCAGGCCG ACGAGGGGCT GAAAACTGTC 
CACCTAGAGC TCACAGAGAC TTGTCTTGAC ATGATGGCCC GATATGTATT TTCCAACTTC 
TCTGCACTTC CCAAGAGGTC TCCCATCGCA GAGTTCCTGT TAGCAGGAGG CCGCAGTATG 
ACCTGGTTGG TGGGCAACAA GTTGGTCACC ATAACAACCA GCGGAGGGGT CAGAACACAA 
GCGCTGCTGG GCCTGGACAT GGCTGATCGC CTGGGAGGAG GAGGAGGGGA ATTGACCAGG 
TCAGACCCAT CACTACACAC CCGAATGACC AAAGAGGCTC CAGCCAAATT GGAGTCACAG 
TCAAGCCAGC AGCACAACAG AGCTGCACGC ATACGAGTTC GCTCCATGTC AGGTGGTCAT 
GCTCTACGTG CCGGTCCAGC TCAGAGTCTC AGTCCTCTGG TGTCTCCCTC TGAGGGTGAG 
TTGACTGCTC CACTCTCTCC CTCCTCTGGT CCTGCTCTGG ATAGTTTAGG TCCATCCTCT 
TCCACCTCTG CCACTCCATC TGTGCCTCCT ACTCTGAAAG ACAACCCCAC CTTGGCTGAA 
TTTGCCCCAA TGCTCACACA AGGCTGGGCA GAGATCTTCA TACGCAGACC GTCAGGTAAC 
ACAAGCTGGC TAATGTGTTT GGAGAACCCA CCTAGCCCTT TCTCATCTGA GCTTGGCAAC 
ATGCCGCTGC AAGAGCTCTC CAGTGTTCTG ATGGCAATGG AGGGAGTGAA GGAACCTCCC 
ACTCAGACAG CCAGTGCTCC TGCTAGCACA GCTGTCCCTG CTCCAGCTCC TGTCTCTGAG 
CCCCTAACCC AAACACACAG CAGTGTTGGA GGAAAGGCAA ACCTGATTCA GCGCTCCAAC 
ACAGTGGGTG GCTCTCTCTG CTCTCTGGGT ATGGGCACTG CCCCCGCAGG CCCACCAGCC 
CCTGGCAGGT TGCACAGGAG CATTTCCTGG GCAGACTCTG TCGTGGTGCT GGAGGAGGGA 
TCAGGAATTG CTACAGCAAA CACGCCGCCT GAATGGCTGG GGAGTGCAGA GTTTGAGCCA 
ATGTTGTCTG ATCCAATCTT CATAGCTGCT GATAAGTTTA CAAAGACTCC TCCTGAAACA 
CTCAGCAGGT CCTCCTCCAC CTCAAGTCAG GATGATGAAA AGTCAACCCT GGAAGAAGTA 
AGTGAGGGAG CAATTCCAAT CGATCAGCCC CCTTTTGGAC CATCCACTCC AGGAAGCCAG 
GGCCCTGACC TTCCCTTCCA GACACACACT CAGTCCCAGG GTCATGGACT CAACAAGTCC 
AGCTCCTCTC CTGAGCTCCA GACATTACCT GAAGCTTTTG CCAAGGCTGC TATGGAGTCA 
GAAAGTGCGA TGGTTGACAC GTCACGGCCT AGAGCACCCT CAGACGGCAT GCCTCAACCC 
CAGCAGCCCC AGTTTGCGAA AGAAAAGGTA GAGGGAAACA CTGGAGTGGA CACTAATGTA 
GCGGGAGGTC CAAGCCCAGC AGGTGAAGGC CAAGCTGTAT CGTCATCTCA GAGTGGAGGA 
GCGGGGATGA GGTTGTCTTT TCCACCAGCA CCAGTACAAT CTGCACCCAT CTCCCCCAGT 
GGAGGCCACC GGCCCAGAGG TCACACAATC TCAGTCTCAG CCCCCTCCTC CAGGAGGGAG 
AGGCGGGCGG AGAGGGACGC TTACCACAGT CGACCTGGTC CCAGCAACAC TGAGAAAATC 
TCTGGACTGA GCCCCAGCTT TGTGTTTCTG CAGCTCTACC ACTCTCCTTT CTTTGGGAAT 
GAAGCCAACA AGCCACTTCT GCTGCCCAAA ACTCAGGTGA TTGACCGTGC AGTAAAGGTT 
CTGGACCAAA TGCCCCCTTA TGACACCCAT AAAATTGGGG TGGTGTTTGT AGGAGCTGGT 
CAGGTTAACA ATGAAGTCGC CATCTTGTCG AACGAGTACG GTTCGAACCG CTACGCCTCT 
TTCCTCACAG GTCTTGGCAA ATTAATCCAT CTGAAAGACT GCGATCCAGA CCAGATTTTT 
CTTGGAGGGC TTGACCAGTA TGGAGATGAT GGAGAGTTCA CCTACTGCTG GCATGACGAC 
ATCATGCAGG CTATCTTTCA CATAGCCACA CTGATGCCAA ACAGGGAGAG CGACAAGGGT 
TGCTGCAACA AAAAGCGACA CATTGGCAAC GATTTTGTCA TGGTGGTGTA CAATGACTCT 
GGAGAGGAGT ACAAACTGGG CACAATTAAG GGTCAGTTTA ATTTTGTCGA AGTCATTATA 
AAACCACTGG ATTATGAATG TAACCTCGTT ACCCTCCAGT GCCGCAAAGA CCTCGAGGGA 
TTAGTAGACA CAACGGTGGC AAAAATTGTT TCAGATCGAA ACCTTCCCCT GTTAGTCAGG 
CAGATGGCTC TGCATGCAAA TATGGCCTCA TTGGTGCACC AGTACAGAGC TAACCCCTCT 
GACGCATACG CCTCTAAGTG GCTAGCAAGG TTACGACACA TCAAGAGGAT CAGGACCAGG 
GCTCAAGAAG CCATCCAGTC TCGCTCAACT CCCGGGATTT CACTGACCCA AGGGCAGAGC 
AAACCTTTGT CACAGAGCAG TGCTCCAACA AATGTGGAAG CCACCGGACA ACGAAAAAGA 
CTGGTGTCCA CTGTAGACGA TTTCACAGAT TTTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tsc2 ( XM_004539038.5 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004539095.2 Maylandia zebra TSC complex subunit 2 (tsc2), transcript variant X3, mRNA.

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CloneID	OMa374612
Clone ID Related Accession (Same CDS sequence)	XM_004539038.5 , XM_004539036.4 , XM_004539037.3
Accession Version	XM_004539038.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-23
Organism	Maylandia zebra(zebra mbuna)
Product	tuberin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036785.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004539038.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAAAC AACCAAGCAA GGAGAGTCTC AAGGACAAGG TGAAGGGGAT CTTTGGGCTC 
GGTACACAGC GTCCTCCGAA CAAACAGAGT GACACTAAAC CCTCCGAGTT TATCATTACG 
GCTGACATCA TCAAGGAGCT CCACCCAGAT TGCGGTCTGA GCAACCGTGT TCGCACTATG 
AACCACATCT GTGAGCTTGC CAAAATGAAA AAGTTTGAGG AGCATGCAGT GGAGGCTGTA 
TGGAAAAATG TGGAAGACAT GCTGACTCCA GAGCAGCCAC CAGAGGCCAG ACATGCTGTC 
CTGCAGCTGC TCAGGGCCAT TATACAGGGG CAGGGTGAGC GACTCGGCCC TCTGAGAGCC 
TACTTTTTCA AGGTAATCAG AGACTACCAG CCGTGCAATG AAGACCTTTC TGACAGACTG 
GAGGTGTTTA AGGCTTTAAC AGAGAATGGA AAGGATATCA CATACCTGGA AGAGGACATA 
GCACGCTTTG TCCTCCTGTG GATGGATATA GGCCTTACAT CTGACTTTCT CCATGTTCTT 
GTTAATCTGG TGAAATTCAA TAGCTGCTAC TTGGACCAAA ATGTTTCCAT CATGGTCCAG 
AAAATTTGCC TGCTGTGCAA CAGAACGACG TCTTCGACTG ATATTGAGGT GGCTCTGCAA 
GTGCTCGATG CAGTTGTGTG TTACAACTGC CTGCCCTCCG ACTCTTTAAC CGTTTTCATA 
ATCACACTCT GCCGTACGGT CAACGTCAAA GAGTTTTGCG AGTCCTGTTG GAAGTTAATG 
AGAAAGGTTT TGGGGACTCA CCTCGGCCAT AGTGCAATTT ACACCATGTG TCGCATTATG 
GAGGAAAGGG TGTATATGGA GGATGCACCG TTGTTAAGAG GAGCAGTTTT CTTTGTTGGA 
ATGGCGCTTT GGGGCGCACA CAGACTGCCT GCACTCAAAA ATACACCGAC GCTTGTTCTG 
CCATCTTTCT ACAAGGCCAT GTCATGTGCC AACGAGGTGG TCTCCTACGA AATTGTTCTT 
TCAATAACAA GACTCATCAA AAAGTACGGT AAAGAGCTAC AAGTGGTCAC ATGGGACATC 
CTACTCGGCA TCATAGAGCG ACTGTTGCAA CAGATTCAGA CAATAGGCAG TGCGGAACTA 
AAGGCCATTG TTTATGAACT GTTGACCACC GTGGAAGAGC TTTATGAACA AAACGGTTAC 
CACGGCTCCA CTGAGAAGTT TTTCAGCCTG GTGGAGAAAT GTGCTGATAA AAGACCAGAT 
GCATCAGTGC TGACACTTAT CTCCTACAGG GCTCAGTCTA TCCAGCCTGC TAAAGAAGGC 
TGGATTCAGA GCCTTCACCG GCTCATGGAG AAATTCTTCA GGAACGAGAC TCGCAGTGTG 
ATAAGAATCA AAGTGCTTCA TATCCTGTCC TTCGTTCTCA GTACCAACCG GCAGCTCTAT 
GAGGATGAGT TGATTGAAAT GGTGGTGATT CCCCAGCTCA GTGGGATTGC TGAAGACCGG 
GACTTGGCAG TTAGAAAACA GGCTACTCAG CTATTAGTAG ACCTCGCTGA GGGCTGCAAC 
ACGCACCACT TCACCAGCCT GCTGGACATC ATTGAACGGG TGTCTAGCCG CTCTCTGGTG 
TGTTCAGGGC CCCTAGAGGT TTCTGAGAGA GACCCCACAG CAGAGTCTCC TATGGAGGAC 
GTCAGGACTG CTATTCTGGG CCTGCTTGAA ATCCTGCAGA GCAAACTTTA CAGTCTGCCA 
GCCAGCCATG CGAGTCGTGT TTATGAGTTG CTTATCAGCC ACCTGCAGCT TCATTACAAG 
AACAAGTACT GTTCAACTAT CGCCTCCACT ATTAGACTAA AGGTGTTTGA CTTCTTCCTC 
ATGATGCGTG CAGACTCTCT TCACCGTCTT GGGGTCCCAA ACAAAGATGG TGTCATGAGA 
TTCAGCCCTT ATTGCTACTG TGATATAGGG GAACCAGAGA AACGTGTTGG TGAAAAGAAG 
CCAGCAGGTT CAACCTCTCC TCCTGCTGGC AGCCCTGCTC CTCCTGCTGC TCCTCCCAGT 
TCAGCAGCCT CAACACGCTC AGCCTACCTT TCCTATTCGC CTGCCTTCAG TGTCCTCCTG 
CAGTGCCTCA AGATGGAGAC GGATTGGAAG GTGCTGAAGC TGGTTCTTGA CAAGCTGCCA 
TGGATGCTGC AGTACAAAGT GTTGCTGCTC ACATCACCAT GCAACTTAGA TCAGCTGTGT 
TCTACACTCT GCTGCATGGT GACAGATCGT CTCATCTCAG AGCGATTGAA AAAGACTCCA 
GAGGGGTTTT CTCGCACAGA TGTTCAGCTG GCAGTGATTC CCGTTCTTAC AGCAATTACC 
TCCTACCACA ACTACTTGGA GCAGTCAAGA CAGAGAGAAC TGGTTCAGTG CCTCGAGACT 
GGGCTCATTT ACCGCTGTGC CAAGCAATGT GTGGTGGCCC TTACAATGTG CACGGTGGAG 
ATGCCTGATA TCATGATCAA GCTTCTTCCT GCTCTTATCG TCAAGCTCAC TCACATCTCT 
GCTACTGTTG CCATGGCATC TCCTATGCTC GAGTTTCTCT CCACCCTCGT GCGCCTTCCC 
CATCTGTATG CCAACTTTGT AGCTGAGCAG TATGTCAGCG TGTTTGCCAT CTCACTACCT 
TACACTAACC CCTCAAAGTT CAACCAGTAC ATTGTGTCCC TGGCCCACCA TGTGATTGCC 
ATGTGGTTCA TACGCTGCAG ACTACCCTTC CGCAAGGACT TTGTTCAGTA TATCACAAAG 
GGTTTGCGTT CCAACGCCCT TCTGCCATTC GATGACAGTC ATGAGCAGAG TCCGTTTCGT 
GCCCGAAGCA CCAGCCTCAA TGAGAGACCC AAGAGCTTGC GGGCAGCAAA AGTGGCAAAG 
GCAGCAGCAG CGGTAGCCAA TAGCAGCAGC TCTCCAGTTA AAGAGCTGAG GGACCTTTCA 
GCCATGGACG CTTTCCGCTC CCGCAGCATC AGCGTCTCCG AACATGCGGT CCGCAGGATG 
CACACCTCAA CCACCACATG CAGTCTGGGC TCTGCCGATG AAAATGCAGT AACTCAGGCC 
GACGAGGGGC TGAAAACTGT CCACCTAGAG CTCACAGAGA CTTGTCTTGA CATGATGGCC 
CGATATGTAT TTTCCAACTT CTCTGCACTT CCCAAGAGGT CTCCCATCGC AGAGTTCCTG 
TTAGCAGGAG GCCGCAGTAT GACCTGGTTG GTGGGCAACA AGTTGGTCAC CATAACAACC 
AGCGGAGGGG TCAGAACACA AGCGCTGCTG GGCCTGGACA TGGCTGATCG CCTGGGAGGA 
GGAGGAGGGG AATTGACCAG GTCAGACCCA TCACTACACA CCCGAATGAC CAAAGAGGCT 
CCAGCCAAAT TGGAGTCACA GTCAAGCCAG CAGCACAACA GAGCTGCACG CATACGAGTT 
CGCTCCATGT CAGGTGGTCA TGCTCTACGT GCCGGTCCAG CTCAGAGTCT CAGTCCTCTG 
GTGTCTCCCT CTGAGGGTGA GTTGACTGCT CCACTCTCTC CCTCCTCTGG TCCTGCTCTG 
GATAGTTTAG GTCCATCCTC TTCCACCTCT GCCACTCCAT CTGTGCCTCC TACTCTGAAA 
GACAACCCCA CCTTGGCTGA ATTTGCCCCA ATGCTCACAC AAGGCTGGGC AGAGATCTTC 
ATACGCAGAC CGTCAGGTAA CACAAGCTGG CTAATGTGTT TGGAGAACCC ACCTAGCCCT 
TTCTCATCTG AGCTTGGCAA CATGCCGCTG CAAGAGCTCT CCAGTGTTCT GATGGCAATG 
GAGGGAGTGA AGGAACCTCC CACTCAGACA GCCAGTGCTC CTGCTAGCAC AGCTGTCCCT 
GCTCCAGCTC CTGTCTCTGA GCCCCTAACC CAAACACACA GCAGTGTTGG AGGAAAGGCA 
AACCTGATTC AGCGCTCCAA CACAGTGGGT GGCTCTCTCT GCTCTCTGGG TATGGGCACT 
GCCCCCCCAG GCCCACCAGC CCCTGGCAGG TTGCACAGGA GCATTTCCTG GGCAGACTCT 
GTCGTGGTGC TGGAGGAGGG ATCAGGAATT GCTACAGCAA ACACGCCGCC TGAATGGCTG 
GGGAGTGCAG AGTTTGAGCC AATGTTGTCT GATCCAATCT TCATAGCTGC TGATAAGTTT 
ACAAAGACTC CTCCTGAAAC ACTCAGCAGG TCCTCCTCCA CCTCAAGTCA GGATGATGAA 
AAGTCAACCC TGGAAGAAGT AAGTGAGGGA GCAATTCCAA TCGATCAGCC CCCTTTTGGA 
CCATCCACTC CAGGAAGCCA GGGGCCTGAC CTTCCCTTCC AGACACACAC TCAGTCCCAG 
GGTCATGGAC TCAACAAGTC CAGCTCCTCT CCTGAGCTCC AGACATTACC TGAAGCTTTT 
GCCAAGGCTG CTATGGAGTC AGAAAGTGCG ATGGTTGACA CGTCACGGCC TAGAGCACCC 
TCAGACGGCA TGCCTCAACC CCAGCAGCCC CAGTTTGCGA AAGAAAAGGT AGAGGGAAAC 
ACTGGAGTGG ACACTAATGT AGCGGGAGGT CCAAGCCCAG CAGGTGAAGG CCAAGCTGTA 
TCGTCATCTC AGAGTGGAGG AGCGGGGATG AGGTTGTCTT TTCCACCAGC ACCAGTACAA 
TCTGCACCCA TCTCCCCCAG TGGAGGCCAC CGGCCCAGAG GTCACACAAT CTCAGTCTCA 
GCCCCCTCCT CCAGGAGGGA GAGGCGGGCG GAGAGGGACG CTTACCACAG TCGACCTGGT 
CCCAGCAACA CTGAGAAAAT CTCTGGACTG AGCCCCAGCT TTGTGTTTCT GCAGCTCTAC 
CACTCTCCTT TCTTTGGGAA TGAAGCCAAC AAGCCACTTC TGCTGCCCAA AACTCAGGTG 
ATTGACCGTG CAGTAAAGGT TCTGGACCAA ATGCCCCCTT ATGACACCCA TAAAATTGGG 
GTGGTGTTTG TAGGAGCTGG TCAGGTTAAC AATGAAGTCG CCATCTTGTC GAACGAGTAC 
GGTTCGAACC GCTACGCCTC TTTCCTCACA GGTCTTGGCA AATTAATCCA TCTGAAAGAC 
TGCGATCCAG ACCAGATTTT TCTTGGAGGG CTTGACCAGT ATGGAGATGA TGGAGAGTTC 
ACCTACTGCT GGCATGACGA CATCATGCAG GCTATCTTTC ACATAGCCAC ACTGATGCCA 
AACAGGGAGA GCGACAAGGG TTGCTGCAAC AAAAAGCGAC ACATTGGCAA CGATTTTGTC 
ATGGTGGTGT ACAATGACTC TGGAGAGGAG TACAAACTGG GCACAATTAA GGGTCAGTTT 
AATTTTGTCG AAGTCATTAT AAAACCACTG GATTATGAAT GTAACCTCGT TACCCTCCAG 
TGCCGCAAAG ACCTCGAGGG ATTAGTAGAC ACAACGGTGG CAAAAATTGT TTCAGATCGA 
AACCTTCCCC TGTTAGTCAG GCAGATGGCT CTGCATGCAA ATATGGCCTC ATTGGTGCAC 
CAGTACAGAG CTAACCCCTC TGACGCATAC GCCTCTAAGT GGCTAGCAAG GTTACGACAC 
ATCAAGAGGA TCAGGACCAG GGCTCAAGAA GCCATCCAGT CTCGCTCAAC TCCCGGGATT 
TCACTGACCC AAGGGCAGAG CAAACCTTTG TCACAGAGCA GTGCTCCAAC AAATGTGGAA 
GCCACCGGAC AACGAAAAAG ACTGGTGTCC ACTGTAGACG ATTTCACAGA TTTTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004539095.2
CDS	71..5770
Translation	MNKQPSKESLKDKVKGIFGLGTQRPPNKQSDTKPSEFIITADIIKELHPDCGLSNRVRTMNHICELAKMKKFEEHAVEAVWKNVEDMLTPEQPPEARHAVLQLLRAIIQGQGERLGPLRAYFFKVIRDYQPCNEDLSDRLEVFKALTENGKDITYLEEDIARFVLLWMDIGLTSDFLHVLVNLVKFNSCYLDQNVSIMVQKICLLCNRTTSSTDIEVALQVLDAVVCYNCLPSDSLTVFIITLCRTVNVKEFCESCWKLMRKVLGTHLGHSAIYTMCRIMEERVYMEDAPLLRGAVFFVGMALWGAHRLPALKNTPTLVLPSFYKAMSCANEVVSYEIVLSITRLIKKYGKELQVVTWDILLGIIERLLQQIQTIGSAELKAIVYELLTTVEELYEQNGYHGSTEKFFSLVEKCADKRPDASVLTLISYRAQSIQPAKEGWIQSLHRLMEKFFRNETRSVIRIKVLHILSFVLSTNRQLYEDELIEMVVIPQLSGIAEDRDLAVRKQATQLLVDLAEGCNTHHFTSLLDIIERVSSRSLVCSGPLEVSERDPTAESPMEDVRTAILGLLEILQSKLYSLPASHASRVYELLISHLQLHYKNKYCSTIASTIRLKVFDFFLMMRADSLHRLGVPNKDGVMRFSPYCYCDIGEPEKRVGEKKPAGSTSPPAGSPAPPAAPPSSAASTRSAYLSYSPAFSVLLQCLKMETDWKVLKLVLDKLPWMLQYKVLLLTSPCNLDQLCSTLCCMVTDRLISERLKKTPEGFSRTDVQLAVIPVLTAITSYHNYLEQSRQRELVQCLETGLIYRCAKQCVVALTMCTVEMPDIMIKLLPALIVKLTHISATVAMASPMLEFLSTLVRLPHLYANFVAEQYVSVFAISLPYTNPSKFNQYIVSLAHHVIAMWFIRCRLPFRKDFVQYITKGLRSNALLPFDDSHEQSPFRARSTSLNERPKSLRAAKVAKAAAAVANSSSSPVKELRDLSAMDAFRSRSISVSEHAVRRMHTSTTTCSLGSADENAVTQADEGLKTVHLELTETCLDMMARYVFSNFSALPKRSPIAEFLLAGGRSMTWLVGNKLVTITTSGGVRTQALLGLDMADRLGGGGGELTRSDPSLHTRMTKEAPAKLESQSSQQHNRAARIRVRSMSGGHALRAGPAQSLSPLVSPSEGELTAPLSPSSGPALDSLGPSSSTSATPSVPPTLKDNPTLAEFAPMLTQGWAEIFIRRPSGNTSWLMCLENPPSPFSSELGNMPLQELSSVLMAMEGVKEPPTQTASAPASTAVPAPAPVSEPLTQTHSSVGGKANLIQRSNTVGGSLCSLGMGTAPPGPPAPGRLHRSISWADSVVVLEEGSGIATANTPPEWLGSAEFEPMLSDPIFIAADKFTKTPPETLSRSSSTSSQDDEKSTLEEVSEGAIPIDQPPFGPSTPGSQGPDLPFQTHTQSQGHGLNKSSSSPELQTLPEAFAKAAMESESAMVDTSRPRAPSDGMPQPQQPQFAKEKVEGNTGVDTNVAGGPSPAGEGQAVSSSQSGGAGMRLSFPPAPVQSAPISPSGGHRPRGHTISVSAPSSRRERRAERDAYHSRPGPSNTEKISGLSPSFVFLQLYHSPFFGNEANKPLLLPKTQVIDRAVKVLDQMPPYDTHKIGVVFVGAGQVNNEVAILSNEYGSNRYASFLTGLGKLIHLKDCDPDQIFLGGLDQYGDDGEFTYCWHDDIMQAIFHIATLMPNRESDKGCCNKKRHIGNDFVMVVYNDSGEEYKLGTIKGQFNFVEVIIKPLDYECNLVTLQCRKDLEGLVDTTVAKIVSDRNLPLLVRQMALHANMASLVHQYRANPSDAYASKWLARLRHIKRIRTRAQEAIQSRSTPGISLTQGQSKPLSQSSAPTNVEATGQRKRLVSTVDDFTDFV

Target ORF information:

RefSeq Version	XM_004539038.5
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra TSC complex subunit 2 (tsc2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004539038.5

ORF Insert Sequence:

ATGAATAAAC AACCAAGCAA GGAGAGTCTC AAGGACAAGG TGAAGGGGAT CTTTGGGCTC 
GGTACACAGC GTCCTCCGAA CAAACAGAGT GACACTAAAC CCTCCGAGTT TATCATTACG 
GCTGACATCA TCAAGGAGCT CCACCCAGAT TGCGGTCTGA GCAACCGTGT TCGCACTATG 
AACCACATCT GTGAGCTTGC CAAAATGAAA AAGTTTGAGG AGCATGCAGT GGAGGCTGTA 
TGGAAAAATG TGGAAGACAT GCTGACTCCA GAGCAGCCAC CAGAGGCCAG ACATGCTGTC 
CTGCAGCTGC TCAGGGCCAT TATACAGGGG CAGGGTGAGC GACTCGGCCC TCTGAGAGCC 
TACTTTTTCA AGGTAATCAG AGACTACCAG CCGTGCAATG AAGACCTTTC TGACAGACTG 
GAGGTGTTTA AGGCTTTAAC AGAGAATGGA AAGGATATCA CATACCTGGA AGAGGACATA 
GCACGCTTTG TCCTCCTGTG GATGGATATA GGCCTTACAT CTGACTTTCT CCATGTTCTT 
GTTAATCTGG TGAAATTCAA TAGCTGCTAC TTGGACCAAA ATGTTTCCAT CATGGTCCAG 
AAAATTTGCC TGCTGTGCAA CAGAACGACG TCTTCGACTG ATATTGAGGT GGCTCTGCAA 
GTGCTCGATG CAGTTGTGTG TTACAACTGC CTGCCCTCCG ACTCTTTAAC CGTTTTCATA 
ATCACACTCT GCCGTACGGT CAACGTCAAA GAGTTTTGCG AGTCCTGTTG GAAGTTAATG 
AGAAAGGTTT TGGGGACTCA CCTCGGCCAT AGTGCAATTT ACACCATGTG TCGCATTATG 
GAGGAAAGGG TGTATATGGA GGATGCACCG TTGTTAAGAG GAGCAGTTTT CTTTGTTGGA 
ATGGCGCTTT GGGGCGCACA CAGACTGCCT GCACTCAAAA ATACACCGAC GCTTGTTCTG 
CCATCTTTCT ACAAGGCCAT GTCATGTGCC AACGAGGTGG TCTCCTACGA AATTGTTCTT 
TCAATAACAA GACTCATCAA AAAGTACGGT AAAGAGCTAC AAGTGGTCAC ATGGGACATC 
CTACTCGGCA TCATAGAGCG ACTGTTGCAA CAGATTCAGA CAATAGGCAG TGCGGAACTA 
AAGGCCATTG TTTATGAACT GTTGACCACC GTGGAAGAGC TTTATGAACA AAACGGTTAC 
CACGGCTCCA CTGAGAAGTT TTTCAGCCTG GTGGAGAAAT GTGCTGATAA AAGACCAGAT 
GCATCAGTGC TGACACTTAT CTCCTACAGG GCTCAGTCTA TCCAGCCTGC TAAAGAAGGC 
TGGATTCAGA GCCTTCACCG GCTCATGGAG AAATTCTTCA GGAACGAGAC TCGCAGTGTG 
ATAAGAATCA AAGTGCTTCA TATCCTGTCC TTCGTTCTCA GTACCAACCG GCAGCTCTAT 
GAGGATGAGT TGATTGAAAT GGTGGTGATT CCCCAGCTCA GTGGGATTGC TGAAGACCGG 
GACTTGGCAG TTAGAAAACA GGCTACTCAG CTATTAGTAG ACCTCGCTGA GGGCTGCAAC 
ACGCACCACT TCACCAGCCT GCTGGACATC ATTGAACGGG TGTCTAGCCG CTCTCTGGTG 
TGTTCAGGGC CCCTAGAGGT TTCTGAGAGA GACCCCACAG CAGAGTCTCC TATGGAGGAC 
GTCAGGACTG CTATTCTGGG CCTGCTTGAA ATCCTGCAGA GCAAACTTTA CAGTCTGCCA 
GCCAGCCATG CGAGTCGTGT TTATGAGTTG CTTATCAGCC ACCTGCAGCT TCATTACAAG 
AACAAGTACT GTTCAACTAT CGCCTCCACT ATTAGACTAA AGGTGTTTGA CTTCTTCCTC 
ATGATGCGTG CAGACTCTCT TCACCGTCTT GGGGTCCCAA ACAAAGATGG TGTCATGAGA 
TTCAGCCCTT ATTGCTACTG TGATATAGGG GAACCAGAGA AACGTGTTGG TGAAAAGAAG 
CCAGCAGGTT CAACCTCTCC TCCTGCTGGC AGCCCTGCTC CTCCTGCTGC TCCTCCCAGT 
TCAGCAGCCT CAACACGCTC AGCCTACCTT TCCTATTCGC CTGCCTTCAG TGTCCTCCTG 
CAGTGCCTCA AGATGGAGAC GGATTGGAAG GTGCTGAAGC TGGTTCTTGA CAAGCTGCCA 
TGGATGCTGC AGTACAAAGT GTTGCTGCTC ACATCACCAT GCAACTTAGA TCAGCTGTGT 
TCTACACTCT GCTGCATGGT GACAGATCGT CTCATCTCAG AGCGATTGAA AAAGACTCCA 
GAGGGGTTTT CTCGCACAGA TGTTCAGCTG GCAGTGATTC CCGTTCTTAC AGCAATTACC 
TCCTACCACA ACTACTTGGA GCAGTCAAGA CAGAGAGAAC TGGTTCAGTG CCTCGAGACT 
GGGCTCATTT ACCGCTGTGC CAAGCAATGT GTGGTGGCCC TTACAATGTG CACGGTGGAG 
ATGCCTGATA TCATGATCAA GCTTCTTCCT GCTCTTATCG TCAAGCTCAC TCACATCTCT 
GCTACTGTTG CCATGGCATC TCCTATGCTC GAGTTTCTCT CCACCCTCGT GCGCCTTCCC 
CATCTGTATG CCAACTTTGT AGCTGAGCAG TATGTCAGCG TGTTTGCCAT CTCACTACCT 
TACACTAACC CCTCAAAGTT CAACCAGTAC ATTGTGTCCC TGGCCCACCA TGTGATTGCC 
ATGTGGTTCA TACGCTGCAG ACTACCCTTC CGCAAGGACT TTGTTCAGTA TATCACAAAG 
GGTTTGCGTT CCAACGCCCT TCTGCCATTC GATGACAGTC ATGAGCAGAG TCCGTTTCGT 
GCCCGAAGCA CCAGCCTCAA TGAGAGACCC AAGAGCTTGC GGGCAGCAAA AGTGGCAAAG 
GCAGCAGCAG CGGTAGCCAA TAGCAGCAGC TCTCCAGTTA AAGAGCTGAG GGACCTTTCA 
GCCATGGACG CTTTCCGCTC CCGCAGCATC AGCGTCTCCG AACATGCGGT CCGCAGGATG 
CACACCTCAA CCACCACATG CAGTCTGGGC TCTGCCGATG AAAATGCAGT AACTCAGGCC 
GACGAGGGGC TGAAAACTGT CCACCTAGAG CTCACAGAGA CTTGTCTTGA CATGATGGCC 
CGATATGTAT TTTCCAACTT CTCTGCACTT CCCAAGAGGT CTCCCATCGC AGAGTTCCTG 
TTAGCAGGAG GCCGCAGTAT GACCTGGTTG GTGGGCAACA AGTTGGTCAC CATAACAACC 
AGCGGAGGGG TCAGAACACA AGCGCTGCTG GGCCTGGACA TGGCTGATCG CCTGGGAGGA 
GGAGGAGGGG AATTGACCAG GTCAGACCCA TCACTACACA CCCGAATGAC CAAAGAGGCT 
CCAGCCAAAT TGGAGTCACA GTCAAGCCAG CAGCACAACA GAGCTGCACG CATACGAGTT 
CGCTCCATGT CAGGTGGTCA TGCTCTACGT GCCGGTCCAG CTCAGAGTCT CAGTCCTCTG 
GTGTCTCCCT CTGAGGGTGA GTTGACTGCT CCACTCTCTC CCTCCTCTGG TCCTGCTCTG 
GATAGTTTAG GTCCATCCTC TTCCACCTCT GCCACTCCAT CTGTGCCTCC TACTCTGAAA 
GACAACCCCA CCTTGGCTGA ATTTGCCCCA ATGCTCACAC AAGGCTGGGC AGAGATCTTC 
ATACGCAGAC CGTCAGGTAA CACAAGCTGG CTAATGTGTT TGGAGAACCC ACCTAGCCCT 
TTCTCATCTG AGCTTGGCAA CATGCCGCTG CAAGAGCTCT CCAGTGTTCT GATGGCAATG 
GAGGGAGTGA AGGAACCTCC CACTCAGACA GCCAGTGCTC CTGCTAGCAC AGCTGTCCCT 
GCTCCAGCTC CTGTCTCTGA GCCCCTAACC CAAACACACA GCAGTGTTGG AGGAAAGGCA 
AACCTGATTC AGCGCTCCAA CACAGTGGGT GGCTCTCTCT GCTCTCTGGG TATGGGCACT 
GCCCCCCCAG GCCCACCAGC CCCTGGCAGG TTGCACAGGA GCATTTCCTG GGCAGACTCT 
GTCGTGGTGC TGGAGGAGGG ATCAGGAATT GCTACAGCAA ACACGCCGCC TGAATGGCTG 
GGGAGTGCAG AGTTTGAGCC AATGTTGTCT GATCCAATCT TCATAGCTGC TGATAAGTTT 
ACAAAGACTC CTCCTGAAAC ACTCAGCAGG TCCTCCTCCA CCTCAAGTCA GGATGATGAA 
AAGTCAACCC TGGAAGAAGT AAGTGAGGGA GCAATTCCAA TCGATCAGCC CCCTTTTGGA 
CCATCCACTC CAGGAAGCCA GGGGCCTGAC CTTCCCTTCC AGACACACAC TCAGTCCCAG 
GGTCATGGAC TCAACAAGTC CAGCTCCTCT CCTGAGCTCC AGACATTACC TGAAGCTTTT 
GCCAAGGCTG CTATGGAGTC AGAAAGTGCG ATGGTTGACA CGTCACGGCC TAGAGCACCC 
TCAGACGGCA TGCCTCAACC CCAGCAGCCC CAGTTTGCGA AAGAAAAGGT AGAGGGAAAC 
ACTGGAGTGG ACACTAATGT AGCGGGAGGT CCAAGCCCAG CAGGTGAAGG CCAAGCTGTA 
TCGTCATCTC AGAGTGGAGG AGCGGGGATG AGGTTGTCTT TTCCACCAGC ACCAGTACAA 
TCTGCACCCA TCTCCCCCAG TGGAGGCCAC CGGCCCAGAG GTCACACAAT CTCAGTCTCA 
GCCCCCTCCT CCAGGAGGGA GAGGCGGGCG GAGAGGGACG CTTACCACAG TCGACCTGGT 
CCCAGCAACA CTGAGAAAAT CTCTGGACTG AGCCCCAGCT TTGTGTTTCT GCAGCTCTAC 
CACTCTCCTT TCTTTGGGAA TGAAGCCAAC AAGCCACTTC TGCTGCCCAA AACTCAGGTG 
ATTGACCGTG CAGTAAAGGT TCTGGACCAA ATGCCCCCTT ATGACACCCA TAAAATTGGG 
GTGGTGTTTG TAGGAGCTGG TCAGGTTAAC AATGAAGTCG CCATCTTGTC GAACGAGTAC 
GGTTCGAACC GCTACGCCTC TTTCCTCACA GGTCTTGGCA AATTAATCCA TCTGAAAGAC 
TGCGATCCAG ACCAGATTTT TCTTGGAGGG CTTGACCAGT ATGGAGATGA TGGAGAGTTC 
ACCTACTGCT GGCATGACGA CATCATGCAG GCTATCTTTC ACATAGCCAC ACTGATGCCA 
AACAGGGAGA GCGACAAGGG TTGCTGCAAC AAAAAGCGAC ACATTGGCAA CGATTTTGTC 
ATGGTGGTGT ACAATGACTC TGGAGAGGAG TACAAACTGG GCACAATTAA GGGTCAGTTT 
AATTTTGTCG AAGTCATTAT AAAACCACTG GATTATGAAT GTAACCTCGT TACCCTCCAG 
TGCCGCAAAG ACCTCGAGGG ATTAGTAGAC ACAACGGTGG CAAAAATTGT TTCAGATCGA 
AACCTTCCCC TGTTAGTCAG GCAGATGGCT CTGCATGCAA ATATGGCCTC ATTGGTGCAC 
CAGTACAGAG CTAACCCCTC TGACGCATAC GCCTCTAAGT GGCTAGCAAG GTTACGACAC 
ATCAAGAGGA TCAGGACCAG GGCTCAAGAA GCCATCCAGT CTCGCTCAAC TCCCGGGATT 
TCACTGACCC AAGGGCAGAG CAAACCTTTG TCACAGAGCA GTGCTCCAAC AAATGTGGAA 
GCCACCGGAC AACGAAAAAG ACTGGTGTCC ACTGTAGACG ATTTCACAGA TTTTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tsc2 ( XM_004539036.4 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004539093.2 Maylandia zebra TSC complex subunit 2 (tsc2), transcript variant X1, mRNA.

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CloneID	OMa374612
Clone ID Related Accession (Same CDS sequence)	XM_004539038.5 , XM_004539036.4 , XM_004539037.3
Accession Version	XM_004539036.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-23
Organism	Maylandia zebra(zebra mbuna)
Product	tuberin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036785.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004539036.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAAAC AACCAAGCAA GGAGAGTCTC AAGGACAAGG TGAAGGGGAT CTTTGGGCTC 
GGTACACAGC GTCCTCCGAA CAAACAGAGT GACACTAAAC CCTCCGAGTT TATCATTACG 
GCTGACATCA TCAAGGAGCT CCACCCAGAT TGCGGTCTGA GCAACCGTGT TCGCACTATG 
AACCACATCT GTGAGCTTGC CAAAATGAAA AAGTTTGAGG AGCATGCAGT GGAGGCTGTA 
TGGAAAAATG TGGAAGACAT GCTGACTCCA GAGCAGCCAC CAGAGGCCAG ACATGCTGTC 
CTGCAGCTGC TCAGGGCCAT TATACAGGGG CAGGGTGAGC GACTCGGCCC TCTGAGAGCC 
TACTTTTTCA AGGTAATCAG AGACTACCAG CCGTGCAATG AAGACCTTTC TGACAGACTG 
GAGGTGTTTA AGGCTTTAAC AGAGAATGGA AAGGATATCA CATACCTGGA AGAGGACATA 
GCACGCTTTG TCCTCCTGTG GATGGATATA GGCCTTACAT CTGACTTTCT CCATGTTCTT 
GTTAATCTGG TGAAATTCAA TAGCTGCTAC TTGGACCAAA ATGTTTCCAT CATGGTCCAG 
AAAATTTGCC TGCTGTGCAA CAGAACGACG TCTTCGACTG ATATTGAGGT GGCTCTGCAA 
GTGCTCGATG CAGTTGTGTG TTACAACTGC CTGCCCTCCG ACTCTTTAAC CGTTTTCATA 
ATCACACTCT GCCGTACGGT CAACGTCAAA GAGTTTTGCG AGTCCTGTTG GAAGTTAATG 
AGAAAGGTTT TGGGGACTCA CCTCGGCCAT AGTGCAATTT ACACCATGTG TCGCATTATG 
GAGGAAAGGG TGTATATGGA GGATGCACCG TTGTTAAGAG GAGCAGTTTT CTTTGTTGGA 
ATGGCGCTTT GGGGCGCACA CAGACTGCCT GCACTCAAAA ATACACCGAC GCTTGTTCTG 
CCATCTTTCT ACAAGGCCAT GTCATGTGCC AACGAGGTGG TCTCCTACGA AATTGTTCTT 
TCAATAACAA GACTCATCAA AAAGTACGGT AAAGAGCTAC AAGTGGTCAC ATGGGACATC 
CTACTCGGCA TCATAGAGCG ACTGTTGCAA CAGATTCAGA CAATAGGCAG TGCGGAACTA 
AAGGCCATTG TTTATGAACT GTTGACCACC GTGGAAGAGC TTTATGAACA AAACGGTTAC 
CACGGCTCCA CTGAGAAGTT TTTCAGCCTG GTGGAGAAAT GTGCTGATAA AAGACCAGAT 
GCATCAGTGC TGACACTTAT CTCCTACAGG GCTCAGTCTA TCCAGCCTGC TAAAGAAGGC 
TGGATTCAGA GCCTTCACCG GCTCATGGAG AAATTCTTCA GGAACGAGAC TCGCAGTGTG 
ATAAGAATCA AAGTGCTTCA TATCCTGTCC TTCGTTCTCA GTACCAACCG GCAGCTCTAT 
GAGGATGAGT TGATTGAAAT GGTGGTGATT CCCCAGCTCA GTGGGATTGC TGAAGACCGG 
GACTTGGCAG TTAGAAAACA GGCTACTCAG CTATTAGTAG ACCTCGCTGA GGGCTGCAAC 
ACGCACCACT TCACCAGCCT GCTGGACATC ATTGAACGGG TGTCTAGCCG CTCTCTGGTG 
TGTTCAGGGC CCCTAGAGGT TTCTGAGAGA GACCCCACAG CAGAGTCTCC TATGGAGGAC 
GTCAGGACTG CTATTCTGGG CCTGCTTGAA ATCCTGCAGA GCAAACTTTA CAGTCTGCCA 
GCCAGCCATG CGAGTCGTGT TTATGAGTTG CTTATCAGCC ACCTGCAGCT TCATTACAAG 
AACAAGTACT GTTCAACTAT CGCCTCCACT ATTAGACTAA AGGTGTTTGA CTTCTTCCTC 
ATGATGCGTG CAGACTCTCT TCACCGTCTT GGGGTCCCAA ACAAAGATGG TGTCATGAGA 
TTCAGCCCTT ATTGCTACTG TGATATAGGG GAACCAGAGA AACGTGTTGG TGAAAAGAAG 
CCAGCAGGTT CAACCTCTCC TCCTGCTGGC AGCCCTGCTC CTCCTGCTGC TCCTCCCAGT 
TCAGCAGCCT CAACACGCTC AGCCTACCTT TCCTATTCGC CTGCCTTCAG TGTCCTCCTG 
CAGTGCCTCA AGATGGAGAC GGATTGGAAG GTGCTGAAGC TGGTTCTTGA CAAGCTGCCA 
TGGATGCTGC AGTACAAAGT GTTGCTGCTC ACATCACCAT GCAACTTAGA TCAGCTGTGT 
TCTACACTCT GCTGCATGGT GACAGATCGT CTCATCTCAG AGCGATTGAA AAAGACTCCA 
GAGGGGTTTT CTCGCACAGA TGTTCAGCTG GCAGTGATTC CCGTTCTTAC AGCAATTACC 
TCCTACCACA ACTACTTGGA GCAGTCAAGA CAGAGAGAAC TGGTTCAGTG CCTCGAGACT 
GGGCTCATTT ACCGCTGTGC CAAGCAATGT GTGGTGGCCC TTACAATGTG CACGGTGGAG 
ATGCCTGATA TCATGATCAA GCTTCTTCCT GCTCTTATCG TCAAGCTCAC TCACATCTCT 
GCTACTGTTG CCATGGCATC TCCTATGCTC GAGTTTCTCT CCACCCTCGT GCGCCTTCCC 
CATCTGTATG CCAACTTTGT AGCTGAGCAG TATGTCAGCG TGTTTGCCAT CTCACTACCT 
TACACTAACC CCTCAAAGTT CAACCAGTAC ATTGTGTCCC TGGCCCACCA TGTGATTGCC 
ATGTGGTTCA TACGCTGCAG ACTACCCTTC CGCAAGGACT TTGTTCAGTA TATCACAAAG 
GGTTTGCGTT CCAACGCCCT TCTGCCATTC GATGACAGTC ATGAGCAGAG TCCGTTTCGT 
GCCCGAAGCA CCAGCCTCAA TGAGAGACCC AAGAGCTTGC GGGCAGCAAA AGTGGCAAAG 
GCAGCAGCAG CGGTAGCCAA TAGCAGCAGC TCTCCAGTTA AAGAGCTGAG GGACCTTTCA 
GCCATGGACG CTTTCCGCTC CCGCAGCATC AGCGTCTCCG AACATGCGGT CCGCAGGATG 
CACACCTCAA CCACCACATG CAGTCTGGGC TCTGCCGATG AAAATGCAGT AACTCAGGCC 
GACGAGGGGC TGAAAACTGT CCACCTAGAG CTCACAGAGA CTTGTCTTGA CATGATGGCC 
CGATATGTAT TTTCCAACTT CTCTGCACTT CCCAAGAGGT CTCCCATCGC AGAGTTCCTG 
TTAGCAGGAG GCCGCAGTAT GACCTGGTTG GTGGGCAACA AGTTGGTCAC CATAACAACC 
AGCGGAGGGG TCAGAACACA AGCGCTGCTG GGCCTGGACA TGGCTGATCG CCTGGGAGGA 
GGAGGAGGGG AATTGACCAG GTCAGACCCA TCACTACACA CCCGAATGAC CAAAGAGGCT 
CCAGCCAAAT TGGAGTCACA GTCAAGCCAG CAGCACAACA GAGCTGCACG CATACGAGTT 
CGCTCCATGT CAGGTGGTCA TGCTCTACGT GCCGGTCCAG CTCAGAGTCT CAGTCCTCTG 
GTGTCTCCCT CTGAGGGTGA GTTGACTGCT CCACTCTCTC CCTCCTCTGG TCCTGCTCTG 
GATAGTTTAG GTCCATCCTC TTCCACCTCT GCCACTCCAT CTGTGCCTCC TACTCTGAAA 
GACAACCCCA CCTTGGCTGA ATTTGCCCCA ATGCTCACAC AAGGCTGGGC AGAGATCTTC 
ATACGCAGAC CGTCAGGTAA CACAAGCTGG CTAATGTGTT TGGAGAACCC ACCTAGCCCT 
TTCTCATCTG AGCTTGGCAA CATGCCGCTG CAAGAGCTCT CCAGTGTTCT GATGGCAATG 
GAGGGAGTGA AGGAACCTCC CACTCAGACA GCCAGTGCTC CTGCTAGCAC AGCTGTCCCT 
GCTCCAGCTC CTGTCTCTGA GCCCCTAACC CAAACACACA GCAGTGTTGG AGGAAAGGCA 
AACCTGATTC AGCGCTCCAA CACAGTGGGT GGCTCTCTCT GCTCTCTGGG TATGGGCACT 
GCCCCCCCAG GCCCACCAGC CCCTGGCAGG TTGCACAGGA GCATTTCCTG GGCAGACTCT 
GTCGTGGTGC TGGAGGAGGG ATCAGGAATT GCTACAGCAA ACACGCCGCC TGAATGGCTG 
GGGAGTGCAG AGTTTGAGCC AATGTTGTCT GATCCAATCT TCATAGCTGC TGATAAGTTT 
ACAAAGACTC CTCCTGAAAC ACTCAGCAGG TCCTCCTCCA CCTCAAGTCA GGATGATGAA 
AAGTCAACCC TGGAAGAAGT AAGTGAGGGA GCAATTCCAA TCGATCAGCC CCCTTTTGGA 
CCATCCACTC CAGGAAGCCA GGGGCCTGAC CTTCCCTTCC AGACACACAC TCAGTCCCAG 
GGTCATGGAC TCAACAAGTC CAGCTCCTCT CCTGAGCTCC AGACATTACC TGAAGCTTTT 
GCCAAGGCTG CTATGGAGTC AGAAAGTGCG ATGGTTGACA CGTCACGGCC TAGAGCACCC 
TCAGACGGCA TGCCTCAACC CCAGCAGCCC CAGTTTGCGA AAGAAAAGGT AGAGGGAAAC 
ACTGGAGTGG ACACTAATGT AGCGGGAGGT CCAAGCCCAG CAGGTGAAGG CCAAGCTGTA 
TCGTCATCTC AGAGTGGAGG AGCGGGGATG AGGTTGTCTT TTCCACCAGC ACCAGTACAA 
TCTGCACCCA TCTCCCCCAG TGGAGGCCAC CGGCCCAGAG GTCACACAAT CTCAGTCTCA 
GCCCCCTCCT CCAGGAGGGA GAGGCGGGCG GAGAGGGACG CTTACCACAG TCGACCTGGT 
CCCAGCAACA CTGAGAAAAT CTCTGGACTG AGCCCCAGCT TTGTGTTTCT GCAGCTCTAC 
CACTCTCCTT TCTTTGGGAA TGAAGCCAAC AAGCCACTTC TGCTGCCCAA AACTCAGGTG 
ATTGACCGTG CAGTAAAGGT TCTGGACCAA ATGCCCCCTT ATGACACCCA TAAAATTGGG 
GTGGTGTTTG TAGGAGCTGG TCAGGTTAAC AATGAAGTCG CCATCTTGTC GAACGAGTAC 
GGTTCGAACC GCTACGCCTC TTTCCTCACA GGTCTTGGCA AATTAATCCA TCTGAAAGAC 
TGCGATCCAG ACCAGATTTT TCTTGGAGGG CTTGACCAGT ATGGAGATGA TGGAGAGTTC 
ACCTACTGCT GGCATGACGA CATCATGCAG GCTATCTTTC ACATAGCCAC ACTGATGCCA 
AACAGGGAGA GCGACAAGGG TTGCTGCAAC AAAAAGCGAC ACATTGGCAA CGATTTTGTC 
ATGGTGGTGT ACAATGACTC TGGAGAGGAG TACAAACTGG GCACAATTAA GGGTCAGTTT 
AATTTTGTCG AAGTCATTAT AAAACCACTG GATTATGAAT GTAACCTCGT TACCCTCCAG 
TGCCGCAAAG ACCTCGAGGG ATTAGTAGAC ACAACGGTGG CAAAAATTGT TTCAGATCGA 
AACCTTCCCC TGTTAGTCAG GCAGATGGCT CTGCATGCAA ATATGGCCTC ATTGGTGCAC 
CAGTACAGAG CTAACCCCTC TGACGCATAC GCCTCTAAGT GGCTAGCAAG GTTACGACAC 
ATCAAGAGGA TCAGGACCAG GGCTCAAGAA GCCATCCAGT CTCGCTCAAC TCCCGGGATT 
TCACTGACCC AAGGGCAGAG CAAACCTTTG TCACAGAGCA GTGCTCCAAC AAATGTGGAA 
GCCACCGGAC AACGAAAAAG ACTGGTGTCC ACTGTAGACG ATTTCACAGA TTTTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004539093.2
CDS	198..5897
Translation	MNKQPSKESLKDKVKGIFGLGTQRPPNKQSDTKPSEFIITADIIKELHPDCGLSNRVRTMNHICELAKMKKFEEHAVEAVWKNVEDMLTPEQPPEARHAVLQLLRAIIQGQGERLGPLRAYFFKVIRDYQPCNEDLSDRLEVFKALTENGKDITYLEEDIARFVLLWMDIGLTSDFLHVLVNLVKFNSCYLDQNVSIMVQKICLLCNRTTSSTDIEVALQVLDAVVCYNCLPSDSLTVFIITLCRTVNVKEFCESCWKLMRKVLGTHLGHSAIYTMCRIMEERVYMEDAPLLRGAVFFVGMALWGAHRLPALKNTPTLVLPSFYKAMSCANEVVSYEIVLSITRLIKKYGKELQVVTWDILLGIIERLLQQIQTIGSAELKAIVYELLTTVEELYEQNGYHGSTEKFFSLVEKCADKRPDASVLTLISYRAQSIQPAKEGWIQSLHRLMEKFFRNETRSVIRIKVLHILSFVLSTNRQLYEDELIEMVVIPQLSGIAEDRDLAVRKQATQLLVDLAEGCNTHHFTSLLDIIERVSSRSLVCSGPLEVSERDPTAESPMEDVRTAILGLLEILQSKLYSLPASHASRVYELLISHLQLHYKNKYCSTIASTIRLKVFDFFLMMRADSLHRLGVPNKDGVMRFSPYCYCDIGEPEKRVGEKKPAGSTSPPAGSPAPPAAPPSSAASTRSAYLSYSPAFSVLLQCLKMETDWKVLKLVLDKLPWMLQYKVLLLTSPCNLDQLCSTLCCMVTDRLISERLKKTPEGFSRTDVQLAVIPVLTAITSYHNYLEQSRQRELVQCLETGLIYRCAKQCVVALTMCTVEMPDIMIKLLPALIVKLTHISATVAMASPMLEFLSTLVRLPHLYANFVAEQYVSVFAISLPYTNPSKFNQYIVSLAHHVIAMWFIRCRLPFRKDFVQYITKGLRSNALLPFDDSHEQSPFRARSTSLNERPKSLRAAKVAKAAAAVANSSSSPVKELRDLSAMDAFRSRSISVSEHAVRRMHTSTTTCSLGSADENAVTQADEGLKTVHLELTETCLDMMARYVFSNFSALPKRSPIAEFLLAGGRSMTWLVGNKLVTITTSGGVRTQALLGLDMADRLGGGGGELTRSDPSLHTRMTKEAPAKLESQSSQQHNRAARIRVRSMSGGHALRAGPAQSLSPLVSPSEGELTAPLSPSSGPALDSLGPSSSTSATPSVPPTLKDNPTLAEFAPMLTQGWAEIFIRRPSGNTSWLMCLENPPSPFSSELGNMPLQELSSVLMAMEGVKEPPTQTASAPASTAVPAPAPVSEPLTQTHSSVGGKANLIQRSNTVGGSLCSLGMGTAPPGPPAPGRLHRSISWADSVVVLEEGSGIATANTPPEWLGSAEFEPMLSDPIFIAADKFTKTPPETLSRSSSTSSQDDEKSTLEEVSEGAIPIDQPPFGPSTPGSQGPDLPFQTHTQSQGHGLNKSSSSPELQTLPEAFAKAAMESESAMVDTSRPRAPSDGMPQPQQPQFAKEKVEGNTGVDTNVAGGPSPAGEGQAVSSSQSGGAGMRLSFPPAPVQSAPISPSGGHRPRGHTISVSAPSSRRERRAERDAYHSRPGPSNTEKISGLSPSFVFLQLYHSPFFGNEANKPLLLPKTQVIDRAVKVLDQMPPYDTHKIGVVFVGAGQVNNEVAILSNEYGSNRYASFLTGLGKLIHLKDCDPDQIFLGGLDQYGDDGEFTYCWHDDIMQAIFHIATLMPNRESDKGCCNKKRHIGNDFVMVVYNDSGEEYKLGTIKGQFNFVEVIIKPLDYECNLVTLQCRKDLEGLVDTTVAKIVSDRNLPLLVRQMALHANMASLVHQYRANPSDAYASKWLARLRHIKRIRTRAQEAIQSRSTPGISLTQGQSKPLSQSSAPTNVEATGQRKRLVSTVDDFTDFV

Target ORF information:

RefSeq Version	XM_004539036.4
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra TSC complex subunit 2 (tsc2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004539036.4

ORF Insert Sequence:

ATGAATAAAC AACCAAGCAA GGAGAGTCTC AAGGACAAGG TGAAGGGGAT CTTTGGGCTC 
GGTACACAGC GTCCTCCGAA CAAACAGAGT GACACTAAAC CCTCCGAGTT TATCATTACG 
GCTGACATCA TCAAGGAGCT CCACCCAGAT TGCGGTCTGA GCAACCGTGT TCGCACTATG 
AACCACATCT GTGAGCTTGC CAAAATGAAA AAGTTTGAGG AGCATGCAGT GGAGGCTGTA 
TGGAAAAATG TGGAAGACAT GCTGACTCCA GAGCAGCCAC CAGAGGCCAG ACATGCTGTC 
CTGCAGCTGC TCAGGGCCAT TATACAGGGG CAGGGTGAGC GACTCGGCCC TCTGAGAGCC 
TACTTTTTCA AGGTAATCAG AGACTACCAG CCGTGCAATG AAGACCTTTC TGACAGACTG 
GAGGTGTTTA AGGCTTTAAC AGAGAATGGA AAGGATATCA CATACCTGGA AGAGGACATA 
GCACGCTTTG TCCTCCTGTG GATGGATATA GGCCTTACAT CTGACTTTCT CCATGTTCTT 
GTTAATCTGG TGAAATTCAA TAGCTGCTAC TTGGACCAAA ATGTTTCCAT CATGGTCCAG 
AAAATTTGCC TGCTGTGCAA CAGAACGACG TCTTCGACTG ATATTGAGGT GGCTCTGCAA 
GTGCTCGATG CAGTTGTGTG TTACAACTGC CTGCCCTCCG ACTCTTTAAC CGTTTTCATA 
ATCACACTCT GCCGTACGGT CAACGTCAAA GAGTTTTGCG AGTCCTGTTG GAAGTTAATG 
AGAAAGGTTT TGGGGACTCA CCTCGGCCAT AGTGCAATTT ACACCATGTG TCGCATTATG 
GAGGAAAGGG TGTATATGGA GGATGCACCG TTGTTAAGAG GAGCAGTTTT CTTTGTTGGA 
ATGGCGCTTT GGGGCGCACA CAGACTGCCT GCACTCAAAA ATACACCGAC GCTTGTTCTG 
CCATCTTTCT ACAAGGCCAT GTCATGTGCC AACGAGGTGG TCTCCTACGA AATTGTTCTT 
TCAATAACAA GACTCATCAA AAAGTACGGT AAAGAGCTAC AAGTGGTCAC ATGGGACATC 
CTACTCGGCA TCATAGAGCG ACTGTTGCAA CAGATTCAGA CAATAGGCAG TGCGGAACTA 
AAGGCCATTG TTTATGAACT GTTGACCACC GTGGAAGAGC TTTATGAACA AAACGGTTAC 
CACGGCTCCA CTGAGAAGTT TTTCAGCCTG GTGGAGAAAT GTGCTGATAA AAGACCAGAT 
GCATCAGTGC TGACACTTAT CTCCTACAGG GCTCAGTCTA TCCAGCCTGC TAAAGAAGGC 
TGGATTCAGA GCCTTCACCG GCTCATGGAG AAATTCTTCA GGAACGAGAC TCGCAGTGTG 
ATAAGAATCA AAGTGCTTCA TATCCTGTCC TTCGTTCTCA GTACCAACCG GCAGCTCTAT 
GAGGATGAGT TGATTGAAAT GGTGGTGATT CCCCAGCTCA GTGGGATTGC TGAAGACCGG 
GACTTGGCAG TTAGAAAACA GGCTACTCAG CTATTAGTAG ACCTCGCTGA GGGCTGCAAC 
ACGCACCACT TCACCAGCCT GCTGGACATC ATTGAACGGG TGTCTAGCCG CTCTCTGGTG 
TGTTCAGGGC CCCTAGAGGT TTCTGAGAGA GACCCCACAG CAGAGTCTCC TATGGAGGAC 
GTCAGGACTG CTATTCTGGG CCTGCTTGAA ATCCTGCAGA GCAAACTTTA CAGTCTGCCA 
GCCAGCCATG CGAGTCGTGT TTATGAGTTG CTTATCAGCC ACCTGCAGCT TCATTACAAG 
AACAAGTACT GTTCAACTAT CGCCTCCACT ATTAGACTAA AGGTGTTTGA CTTCTTCCTC 
ATGATGCGTG CAGACTCTCT TCACCGTCTT GGGGTCCCAA ACAAAGATGG TGTCATGAGA 
TTCAGCCCTT ATTGCTACTG TGATATAGGG GAACCAGAGA AACGTGTTGG TGAAAAGAAG 
CCAGCAGGTT CAACCTCTCC TCCTGCTGGC AGCCCTGCTC CTCCTGCTGC TCCTCCCAGT 
TCAGCAGCCT CAACACGCTC AGCCTACCTT TCCTATTCGC CTGCCTTCAG TGTCCTCCTG 
CAGTGCCTCA AGATGGAGAC GGATTGGAAG GTGCTGAAGC TGGTTCTTGA CAAGCTGCCA 
TGGATGCTGC AGTACAAAGT GTTGCTGCTC ACATCACCAT GCAACTTAGA TCAGCTGTGT 
TCTACACTCT GCTGCATGGT GACAGATCGT CTCATCTCAG AGCGATTGAA AAAGACTCCA 
GAGGGGTTTT CTCGCACAGA TGTTCAGCTG GCAGTGATTC CCGTTCTTAC AGCAATTACC 
TCCTACCACA ACTACTTGGA GCAGTCAAGA CAGAGAGAAC TGGTTCAGTG CCTCGAGACT 
GGGCTCATTT ACCGCTGTGC CAAGCAATGT GTGGTGGCCC TTACAATGTG CACGGTGGAG 
ATGCCTGATA TCATGATCAA GCTTCTTCCT GCTCTTATCG TCAAGCTCAC TCACATCTCT 
GCTACTGTTG CCATGGCATC TCCTATGCTC GAGTTTCTCT CCACCCTCGT GCGCCTTCCC 
CATCTGTATG CCAACTTTGT AGCTGAGCAG TATGTCAGCG TGTTTGCCAT CTCACTACCT 
TACACTAACC CCTCAAAGTT CAACCAGTAC ATTGTGTCCC TGGCCCACCA TGTGATTGCC 
ATGTGGTTCA TACGCTGCAG ACTACCCTTC CGCAAGGACT TTGTTCAGTA TATCACAAAG 
GGTTTGCGTT CCAACGCCCT TCTGCCATTC GATGACAGTC ATGAGCAGAG TCCGTTTCGT 
GCCCGAAGCA CCAGCCTCAA TGAGAGACCC AAGAGCTTGC GGGCAGCAAA AGTGGCAAAG 
GCAGCAGCAG CGGTAGCCAA TAGCAGCAGC TCTCCAGTTA AAGAGCTGAG GGACCTTTCA 
GCCATGGACG CTTTCCGCTC CCGCAGCATC AGCGTCTCCG AACATGCGGT CCGCAGGATG 
CACACCTCAA CCACCACATG CAGTCTGGGC TCTGCCGATG AAAATGCAGT AACTCAGGCC 
GACGAGGGGC TGAAAACTGT CCACCTAGAG CTCACAGAGA CTTGTCTTGA CATGATGGCC 
CGATATGTAT TTTCCAACTT CTCTGCACTT CCCAAGAGGT CTCCCATCGC AGAGTTCCTG 
TTAGCAGGAG GCCGCAGTAT GACCTGGTTG GTGGGCAACA AGTTGGTCAC CATAACAACC 
AGCGGAGGGG TCAGAACACA AGCGCTGCTG GGCCTGGACA TGGCTGATCG CCTGGGAGGA 
GGAGGAGGGG AATTGACCAG GTCAGACCCA TCACTACACA CCCGAATGAC CAAAGAGGCT 
CCAGCCAAAT TGGAGTCACA GTCAAGCCAG CAGCACAACA GAGCTGCACG CATACGAGTT 
CGCTCCATGT CAGGTGGTCA TGCTCTACGT GCCGGTCCAG CTCAGAGTCT CAGTCCTCTG 
GTGTCTCCCT CTGAGGGTGA GTTGACTGCT CCACTCTCTC CCTCCTCTGG TCCTGCTCTG 
GATAGTTTAG GTCCATCCTC TTCCACCTCT GCCACTCCAT CTGTGCCTCC TACTCTGAAA 
GACAACCCCA CCTTGGCTGA ATTTGCCCCA ATGCTCACAC AAGGCTGGGC AGAGATCTTC 
ATACGCAGAC CGTCAGGTAA CACAAGCTGG CTAATGTGTT TGGAGAACCC ACCTAGCCCT 
TTCTCATCTG AGCTTGGCAA CATGCCGCTG CAAGAGCTCT CCAGTGTTCT GATGGCAATG 
GAGGGAGTGA AGGAACCTCC CACTCAGACA GCCAGTGCTC CTGCTAGCAC AGCTGTCCCT 
GCTCCAGCTC CTGTCTCTGA GCCCCTAACC CAAACACACA GCAGTGTTGG AGGAAAGGCA 
AACCTGATTC AGCGCTCCAA CACAGTGGGT GGCTCTCTCT GCTCTCTGGG TATGGGCACT 
GCCCCCCCAG GCCCACCAGC CCCTGGCAGG TTGCACAGGA GCATTTCCTG GGCAGACTCT 
GTCGTGGTGC TGGAGGAGGG ATCAGGAATT GCTACAGCAA ACACGCCGCC TGAATGGCTG 
GGGAGTGCAG AGTTTGAGCC AATGTTGTCT GATCCAATCT TCATAGCTGC TGATAAGTTT 
ACAAAGACTC CTCCTGAAAC ACTCAGCAGG TCCTCCTCCA CCTCAAGTCA GGATGATGAA 
AAGTCAACCC TGGAAGAAGT AAGTGAGGGA GCAATTCCAA TCGATCAGCC CCCTTTTGGA 
CCATCCACTC CAGGAAGCCA GGGGCCTGAC CTTCCCTTCC AGACACACAC TCAGTCCCAG 
GGTCATGGAC TCAACAAGTC CAGCTCCTCT CCTGAGCTCC AGACATTACC TGAAGCTTTT 
GCCAAGGCTG CTATGGAGTC AGAAAGTGCG ATGGTTGACA CGTCACGGCC TAGAGCACCC 
TCAGACGGCA TGCCTCAACC CCAGCAGCCC CAGTTTGCGA AAGAAAAGGT AGAGGGAAAC 
ACTGGAGTGG ACACTAATGT AGCGGGAGGT CCAAGCCCAG CAGGTGAAGG CCAAGCTGTA 
TCGTCATCTC AGAGTGGAGG AGCGGGGATG AGGTTGTCTT TTCCACCAGC ACCAGTACAA 
TCTGCACCCA TCTCCCCCAG TGGAGGCCAC CGGCCCAGAG GTCACACAAT CTCAGTCTCA 
GCCCCCTCCT CCAGGAGGGA GAGGCGGGCG GAGAGGGACG CTTACCACAG TCGACCTGGT 
CCCAGCAACA CTGAGAAAAT CTCTGGACTG AGCCCCAGCT TTGTGTTTCT GCAGCTCTAC 
CACTCTCCTT TCTTTGGGAA TGAAGCCAAC AAGCCACTTC TGCTGCCCAA AACTCAGGTG 
ATTGACCGTG CAGTAAAGGT TCTGGACCAA ATGCCCCCTT ATGACACCCA TAAAATTGGG 
GTGGTGTTTG TAGGAGCTGG TCAGGTTAAC AATGAAGTCG CCATCTTGTC GAACGAGTAC 
GGTTCGAACC GCTACGCCTC TTTCCTCACA GGTCTTGGCA AATTAATCCA TCTGAAAGAC 
TGCGATCCAG ACCAGATTTT TCTTGGAGGG CTTGACCAGT ATGGAGATGA TGGAGAGTTC 
ACCTACTGCT GGCATGACGA CATCATGCAG GCTATCTTTC ACATAGCCAC ACTGATGCCA 
AACAGGGAGA GCGACAAGGG TTGCTGCAAC AAAAAGCGAC ACATTGGCAA CGATTTTGTC 
ATGGTGGTGT ACAATGACTC TGGAGAGGAG TACAAACTGG GCACAATTAA GGGTCAGTTT 
AATTTTGTCG AAGTCATTAT AAAACCACTG GATTATGAAT GTAACCTCGT TACCCTCCAG 
TGCCGCAAAG ACCTCGAGGG ATTAGTAGAC ACAACGGTGG CAAAAATTGT TTCAGATCGA 
AACCTTCCCC TGTTAGTCAG GCAGATGGCT CTGCATGCAA ATATGGCCTC ATTGGTGCAC 
CAGTACAGAG CTAACCCCTC TGACGCATAC GCCTCTAAGT GGCTAGCAAG GTTACGACAC 
ATCAAGAGGA TCAGGACCAG GGCTCAAGAA GCCATCCAGT CTCGCTCAAC TCCCGGGATT 
TCACTGACCC AAGGGCAGAG CAAACCTTTG TCACAGAGCA GTGCTCCAAC AAATGTGGAA 
GCCACCGGAC AACGAAAAAG ACTGGTGTCC ACTGTAGACG ATTTCACAGA TTTTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tsc2 ( XM_004539037.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004539094.2 Maylandia zebra TSC complex subunit 2 (tsc2), transcript variant X2, mRNA.

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CloneID	OMa374612
Clone ID Related Accession (Same CDS sequence)	XM_004539038.5 , XM_004539036.4 , XM_004539037.3
Accession Version	XM_004539037.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-23
Organism	Maylandia zebra(zebra mbuna)
Product	tuberin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036785.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004539037.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAAAC AACCAAGCAA GGAGAGTCTC AAGGACAAGG TGAAGGGGAT CTTTGGGCTC 
GGTACACAGC GTCCTCCGAA CAAACAGAGT GACACTAAAC CCTCCGAGTT TATCATTACG 
GCTGACATCA TCAAGGAGCT CCACCCAGAT TGCGGTCTGA GCAACCGTGT TCGCACTATG 
AACCACATCT GTGAGCTTGC CAAAATGAAA AAGTTTGAGG AGCATGCAGT GGAGGCTGTA 
TGGAAAAATG TGGAAGACAT GCTGACTCCA GAGCAGCCAC CAGAGGCCAG ACATGCTGTC 
CTGCAGCTGC TCAGGGCCAT TATACAGGGG CAGGGTGAGC GACTCGGCCC TCTGAGAGCC 
TACTTTTTCA AGGTAATCAG AGACTACCAG CCGTGCAATG AAGACCTTTC TGACAGACTG 
GAGGTGTTTA AGGCTTTAAC AGAGAATGGA AAGGATATCA CATACCTGGA AGAGGACATA 
GCACGCTTTG TCCTCCTGTG GATGGATATA GGCCTTACAT CTGACTTTCT CCATGTTCTT 
GTTAATCTGG TGAAATTCAA TAGCTGCTAC TTGGACCAAA ATGTTTCCAT CATGGTCCAG 
AAAATTTGCC TGCTGTGCAA CAGAACGACG TCTTCGACTG ATATTGAGGT GGCTCTGCAA 
GTGCTCGATG CAGTTGTGTG TTACAACTGC CTGCCCTCCG ACTCTTTAAC CGTTTTCATA 
ATCACACTCT GCCGTACGGT CAACGTCAAA GAGTTTTGCG AGTCCTGTTG GAAGTTAATG 
AGAAAGGTTT TGGGGACTCA CCTCGGCCAT AGTGCAATTT ACACCATGTG TCGCATTATG 
GAGGAAAGGG TGTATATGGA GGATGCACCG TTGTTAAGAG GAGCAGTTTT CTTTGTTGGA 
ATGGCGCTTT GGGGCGCACA CAGACTGCCT GCACTCAAAA ATACACCGAC GCTTGTTCTG 
CCATCTTTCT ACAAGGCCAT GTCATGTGCC AACGAGGTGG TCTCCTACGA AATTGTTCTT 
TCAATAACAA GACTCATCAA AAAGTACGGT AAAGAGCTAC AAGTGGTCAC ATGGGACATC 
CTACTCGGCA TCATAGAGCG ACTGTTGCAA CAGATTCAGA CAATAGGCAG TGCGGAACTA 
AAGGCCATTG TTTATGAACT GTTGACCACC GTGGAAGAGC TTTATGAACA AAACGGTTAC 
CACGGCTCCA CTGAGAAGTT TTTCAGCCTG GTGGAGAAAT GTGCTGATAA AAGACCAGAT 
GCATCAGTGC TGACACTTAT CTCCTACAGG GCTCAGTCTA TCCAGCCTGC TAAAGAAGGC 
TGGATTCAGA GCCTTCACCG GCTCATGGAG AAATTCTTCA GGAACGAGAC TCGCAGTGTG 
ATAAGAATCA AAGTGCTTCA TATCCTGTCC TTCGTTCTCA GTACCAACCG GCAGCTCTAT 
GAGGATGAGT TGATTGAAAT GGTGGTGATT CCCCAGCTCA GTGGGATTGC TGAAGACCGG 
GACTTGGCAG TTAGAAAACA GGCTACTCAG CTATTAGTAG ACCTCGCTGA GGGCTGCAAC 
ACGCACCACT TCACCAGCCT GCTGGACATC ATTGAACGGG TGTCTAGCCG CTCTCTGGTG 
TGTTCAGGGC CCCTAGAGGT TTCTGAGAGA GACCCCACAG CAGAGTCTCC TATGGAGGAC 
GTCAGGACTG CTATTCTGGG CCTGCTTGAA ATCCTGCAGA GCAAACTTTA CAGTCTGCCA 
GCCAGCCATG CGAGTCGTGT TTATGAGTTG CTTATCAGCC ACCTGCAGCT TCATTACAAG 
AACAAGTACT GTTCAACTAT CGCCTCCACT ATTAGACTAA AGGTGTTTGA CTTCTTCCTC 
ATGATGCGTG CAGACTCTCT TCACCGTCTT GGGGTCCCAA ACAAAGATGG TGTCATGAGA 
TTCAGCCCTT ATTGCTACTG TGATATAGGG GAACCAGAGA AACGTGTTGG TGAAAAGAAG 
CCAGCAGGTT CAACCTCTCC TCCTGCTGGC AGCCCTGCTC CTCCTGCTGC TCCTCCCAGT 
TCAGCAGCCT CAACACGCTC AGCCTACCTT TCCTATTCGC CTGCCTTCAG TGTCCTCCTG 
CAGTGCCTCA AGATGGAGAC GGATTGGAAG GTGCTGAAGC TGGTTCTTGA CAAGCTGCCA 
TGGATGCTGC AGTACAAAGT GTTGCTGCTC ACATCACCAT GCAACTTAGA TCAGCTGTGT 
TCTACACTCT GCTGCATGGT GACAGATCGT CTCATCTCAG AGCGATTGAA AAAGACTCCA 
GAGGGGTTTT CTCGCACAGA TGTTCAGCTG GCAGTGATTC CCGTTCTTAC AGCAATTACC 
TCCTACCACA ACTACTTGGA GCAGTCAAGA CAGAGAGAAC TGGTTCAGTG CCTCGAGACT 
GGGCTCATTT ACCGCTGTGC CAAGCAATGT GTGGTGGCCC TTACAATGTG CACGGTGGAG 
ATGCCTGATA TCATGATCAA GCTTCTTCCT GCTCTTATCG TCAAGCTCAC TCACATCTCT 
GCTACTGTTG CCATGGCATC TCCTATGCTC GAGTTTCTCT CCACCCTCGT GCGCCTTCCC 
CATCTGTATG CCAACTTTGT AGCTGAGCAG TATGTCAGCG TGTTTGCCAT CTCACTACCT 
TACACTAACC CCTCAAAGTT CAACCAGTAC ATTGTGTCCC TGGCCCACCA TGTGATTGCC 
ATGTGGTTCA TACGCTGCAG ACTACCCTTC CGCAAGGACT TTGTTCAGTA TATCACAAAG 
GGTTTGCGTT CCAACGCCCT TCTGCCATTC GATGACAGTC ATGAGCAGAG TCCGTTTCGT 
GCCCGAAGCA CCAGCCTCAA TGAGAGACCC AAGAGCTTGC GGGCAGCAAA AGTGGCAAAG 
GCAGCAGCAG CGGTAGCCAA TAGCAGCAGC TCTCCAGTTA AAGAGCTGAG GGACCTTTCA 
GCCATGGACG CTTTCCGCTC CCGCAGCATC AGCGTCTCCG AACATGCGGT CCGCAGGATG 
CACACCTCAA CCACCACATG CAGTCTGGGC TCTGCCGATG AAAATGCAGT AACTCAGGCC 
GACGAGGGGC TGAAAACTGT CCACCTAGAG CTCACAGAGA CTTGTCTTGA CATGATGGCC 
CGATATGTAT TTTCCAACTT CTCTGCACTT CCCAAGAGGT CTCCCATCGC AGAGTTCCTG 
TTAGCAGGAG GCCGCAGTAT GACCTGGTTG GTGGGCAACA AGTTGGTCAC CATAACAACC 
AGCGGAGGGG TCAGAACACA AGCGCTGCTG GGCCTGGACA TGGCTGATCG CCTGGGAGGA 
GGAGGAGGGG AATTGACCAG GTCAGACCCA TCACTACACA CCCGAATGAC CAAAGAGGCT 
CCAGCCAAAT TGGAGTCACA GTCAAGCCAG CAGCACAACA GAGCTGCACG CATACGAGTT 
CGCTCCATGT CAGGTGGTCA TGCTCTACGT GCCGGTCCAG CTCAGAGTCT CAGTCCTCTG 
GTGTCTCCCT CTGAGGGTGA GTTGACTGCT CCACTCTCTC CCTCCTCTGG TCCTGCTCTG 
GATAGTTTAG GTCCATCCTC TTCCACCTCT GCCACTCCAT CTGTGCCTCC TACTCTGAAA 
GACAACCCCA CCTTGGCTGA ATTTGCCCCA ATGCTCACAC AAGGCTGGGC AGAGATCTTC 
ATACGCAGAC CGTCAGGTAA CACAAGCTGG CTAATGTGTT TGGAGAACCC ACCTAGCCCT 
TTCTCATCTG AGCTTGGCAA CATGCCGCTG CAAGAGCTCT CCAGTGTTCT GATGGCAATG 
GAGGGAGTGA AGGAACCTCC CACTCAGACA GCCAGTGCTC CTGCTAGCAC AGCTGTCCCT 
GCTCCAGCTC CTGTCTCTGA GCCCCTAACC CAAACACACA GCAGTGTTGG AGGAAAGGCA 
AACCTGATTC AGCGCTCCAA CACAGTGGGT GGCTCTCTCT GCTCTCTGGG TATGGGCACT 
GCCCCCCCAG GCCCACCAGC CCCTGGCAGG TTGCACAGGA GCATTTCCTG GGCAGACTCT 
GTCGTGGTGC TGGAGGAGGG ATCAGGAATT GCTACAGCAA ACACGCCGCC TGAATGGCTG 
GGGAGTGCAG AGTTTGAGCC AATGTTGTCT GATCCAATCT TCATAGCTGC TGATAAGTTT 
ACAAAGACTC CTCCTGAAAC ACTCAGCAGG TCCTCCTCCA CCTCAAGTCA GGATGATGAA 
AAGTCAACCC TGGAAGAAGT AAGTGAGGGA GCAATTCCAA TCGATCAGCC CCCTTTTGGA 
CCATCCACTC CAGGAAGCCA GGGGCCTGAC CTTCCCTTCC AGACACACAC TCAGTCCCAG 
GGTCATGGAC TCAACAAGTC CAGCTCCTCT CCTGAGCTCC AGACATTACC TGAAGCTTTT 
GCCAAGGCTG CTATGGAGTC AGAAAGTGCG ATGGTTGACA CGTCACGGCC TAGAGCACCC 
TCAGACGGCA TGCCTCAACC CCAGCAGCCC CAGTTTGCGA AAGAAAAGGT AGAGGGAAAC 
ACTGGAGTGG ACACTAATGT AGCGGGAGGT CCAAGCCCAG CAGGTGAAGG CCAAGCTGTA 
TCGTCATCTC AGAGTGGAGG AGCGGGGATG AGGTTGTCTT TTCCACCAGC ACCAGTACAA 
TCTGCACCCA TCTCCCCCAG TGGAGGCCAC CGGCCCAGAG GTCACACAAT CTCAGTCTCA 
GCCCCCTCCT CCAGGAGGGA GAGGCGGGCG GAGAGGGACG CTTACCACAG TCGACCTGGT 
CCCAGCAACA CTGAGAAAAT CTCTGGACTG AGCCCCAGCT TTGTGTTTCT GCAGCTCTAC 
CACTCTCCTT TCTTTGGGAA TGAAGCCAAC AAGCCACTTC TGCTGCCCAA AACTCAGGTG 
ATTGACCGTG CAGTAAAGGT TCTGGACCAA ATGCCCCCTT ATGACACCCA TAAAATTGGG 
GTGGTGTTTG TAGGAGCTGG TCAGGTTAAC AATGAAGTCG CCATCTTGTC GAACGAGTAC 
GGTTCGAACC GCTACGCCTC TTTCCTCACA GGTCTTGGCA AATTAATCCA TCTGAAAGAC 
TGCGATCCAG ACCAGATTTT TCTTGGAGGG CTTGACCAGT ATGGAGATGA TGGAGAGTTC 
ACCTACTGCT GGCATGACGA CATCATGCAG GCTATCTTTC ACATAGCCAC ACTGATGCCA 
AACAGGGAGA GCGACAAGGG TTGCTGCAAC AAAAAGCGAC ACATTGGCAA CGATTTTGTC 
ATGGTGGTGT ACAATGACTC TGGAGAGGAG TACAAACTGG GCACAATTAA GGGTCAGTTT 
AATTTTGTCG AAGTCATTAT AAAACCACTG GATTATGAAT GTAACCTCGT TACCCTCCAG 
TGCCGCAAAG ACCTCGAGGG ATTAGTAGAC ACAACGGTGG CAAAAATTGT TTCAGATCGA 
AACCTTCCCC TGTTAGTCAG GCAGATGGCT CTGCATGCAA ATATGGCCTC ATTGGTGCAC 
CAGTACAGAG CTAACCCCTC TGACGCATAC GCCTCTAAGT GGCTAGCAAG GTTACGACAC 
ATCAAGAGGA TCAGGACCAG GGCTCAAGAA GCCATCCAGT CTCGCTCAAC TCCCGGGATT 
TCACTGACCC AAGGGCAGAG CAAACCTTTG TCACAGAGCA GTGCTCCAAC AAATGTGGAA 
GCCACCGGAC AACGAAAAAG ACTGGTGTCC ACTGTAGACG ATTTCACAGA TTTTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004539094.2
CDS	139..5838
Translation	MNKQPSKESLKDKVKGIFGLGTQRPPNKQSDTKPSEFIITADIIKELHPDCGLSNRVRTMNHICELAKMKKFEEHAVEAVWKNVEDMLTPEQPPEARHAVLQLLRAIIQGQGERLGPLRAYFFKVIRDYQPCNEDLSDRLEVFKALTENGKDITYLEEDIARFVLLWMDIGLTSDFLHVLVNLVKFNSCYLDQNVSIMVQKICLLCNRTTSSTDIEVALQVLDAVVCYNCLPSDSLTVFIITLCRTVNVKEFCESCWKLMRKVLGTHLGHSAIYTMCRIMEERVYMEDAPLLRGAVFFVGMALWGAHRLPALKNTPTLVLPSFYKAMSCANEVVSYEIVLSITRLIKKYGKELQVVTWDILLGIIERLLQQIQTIGSAELKAIVYELLTTVEELYEQNGYHGSTEKFFSLVEKCADKRPDASVLTLISYRAQSIQPAKEGWIQSLHRLMEKFFRNETRSVIRIKVLHILSFVLSTNRQLYEDELIEMVVIPQLSGIAEDRDLAVRKQATQLLVDLAEGCNTHHFTSLLDIIERVSSRSLVCSGPLEVSERDPTAESPMEDVRTAILGLLEILQSKLYSLPASHASRVYELLISHLQLHYKNKYCSTIASTIRLKVFDFFLMMRADSLHRLGVPNKDGVMRFSPYCYCDIGEPEKRVGEKKPAGSTSPPAGSPAPPAAPPSSAASTRSAYLSYSPAFSVLLQCLKMETDWKVLKLVLDKLPWMLQYKVLLLTSPCNLDQLCSTLCCMVTDRLISERLKKTPEGFSRTDVQLAVIPVLTAITSYHNYLEQSRQRELVQCLETGLIYRCAKQCVVALTMCTVEMPDIMIKLLPALIVKLTHISATVAMASPMLEFLSTLVRLPHLYANFVAEQYVSVFAISLPYTNPSKFNQYIVSLAHHVIAMWFIRCRLPFRKDFVQYITKGLRSNALLPFDDSHEQSPFRARSTSLNERPKSLRAAKVAKAAAAVANSSSSPVKELRDLSAMDAFRSRSISVSEHAVRRMHTSTTTCSLGSADENAVTQADEGLKTVHLELTETCLDMMARYVFSNFSALPKRSPIAEFLLAGGRSMTWLVGNKLVTITTSGGVRTQALLGLDMADRLGGGGGELTRSDPSLHTRMTKEAPAKLESQSSQQHNRAARIRVRSMSGGHALRAGPAQSLSPLVSPSEGELTAPLSPSSGPALDSLGPSSSTSATPSVPPTLKDNPTLAEFAPMLTQGWAEIFIRRPSGNTSWLMCLENPPSPFSSELGNMPLQELSSVLMAMEGVKEPPTQTASAPASTAVPAPAPVSEPLTQTHSSVGGKANLIQRSNTVGGSLCSLGMGTAPPGPPAPGRLHRSISWADSVVVLEEGSGIATANTPPEWLGSAEFEPMLSDPIFIAADKFTKTPPETLSRSSSTSSQDDEKSTLEEVSEGAIPIDQPPFGPSTPGSQGPDLPFQTHTQSQGHGLNKSSSSPELQTLPEAFAKAAMESESAMVDTSRPRAPSDGMPQPQQPQFAKEKVEGNTGVDTNVAGGPSPAGEGQAVSSSQSGGAGMRLSFPPAPVQSAPISPSGGHRPRGHTISVSAPSSRRERRAERDAYHSRPGPSNTEKISGLSPSFVFLQLYHSPFFGNEANKPLLLPKTQVIDRAVKVLDQMPPYDTHKIGVVFVGAGQVNNEVAILSNEYGSNRYASFLTGLGKLIHLKDCDPDQIFLGGLDQYGDDGEFTYCWHDDIMQAIFHIATLMPNRESDKGCCNKKRHIGNDFVMVVYNDSGEEYKLGTIKGQFNFVEVIIKPLDYECNLVTLQCRKDLEGLVDTTVAKIVSDRNLPLLVRQMALHANMASLVHQYRANPSDAYASKWLARLRHIKRIRTRAQEAIQSRSTPGISLTQGQSKPLSQSSAPTNVEATGQRKRLVSTVDDFTDFV

Target ORF information:

RefSeq Version	XM_004539037.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra TSC complex subunit 2 (tsc2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004539037.3

ORF Insert Sequence:

ATGAATAAAC AACCAAGCAA GGAGAGTCTC AAGGACAAGG TGAAGGGGAT CTTTGGGCTC 
GGTACACAGC GTCCTCCGAA CAAACAGAGT GACACTAAAC CCTCCGAGTT TATCATTACG 
GCTGACATCA TCAAGGAGCT CCACCCAGAT TGCGGTCTGA GCAACCGTGT TCGCACTATG 
AACCACATCT GTGAGCTTGC CAAAATGAAA AAGTTTGAGG AGCATGCAGT GGAGGCTGTA 
TGGAAAAATG TGGAAGACAT GCTGACTCCA GAGCAGCCAC CAGAGGCCAG ACATGCTGTC 
CTGCAGCTGC TCAGGGCCAT TATACAGGGG CAGGGTGAGC GACTCGGCCC TCTGAGAGCC 
TACTTTTTCA AGGTAATCAG AGACTACCAG CCGTGCAATG AAGACCTTTC TGACAGACTG 
GAGGTGTTTA AGGCTTTAAC AGAGAATGGA AAGGATATCA CATACCTGGA AGAGGACATA 
GCACGCTTTG TCCTCCTGTG GATGGATATA GGCCTTACAT CTGACTTTCT CCATGTTCTT 
GTTAATCTGG TGAAATTCAA TAGCTGCTAC TTGGACCAAA ATGTTTCCAT CATGGTCCAG 
AAAATTTGCC TGCTGTGCAA CAGAACGACG TCTTCGACTG ATATTGAGGT GGCTCTGCAA 
GTGCTCGATG CAGTTGTGTG TTACAACTGC CTGCCCTCCG ACTCTTTAAC CGTTTTCATA 
ATCACACTCT GCCGTACGGT CAACGTCAAA GAGTTTTGCG AGTCCTGTTG GAAGTTAATG 
AGAAAGGTTT TGGGGACTCA CCTCGGCCAT AGTGCAATTT ACACCATGTG TCGCATTATG 
GAGGAAAGGG TGTATATGGA GGATGCACCG TTGTTAAGAG GAGCAGTTTT CTTTGTTGGA 
ATGGCGCTTT GGGGCGCACA CAGACTGCCT GCACTCAAAA ATACACCGAC GCTTGTTCTG 
CCATCTTTCT ACAAGGCCAT GTCATGTGCC AACGAGGTGG TCTCCTACGA AATTGTTCTT 
TCAATAACAA GACTCATCAA AAAGTACGGT AAAGAGCTAC AAGTGGTCAC ATGGGACATC 
CTACTCGGCA TCATAGAGCG ACTGTTGCAA CAGATTCAGA CAATAGGCAG TGCGGAACTA 
AAGGCCATTG TTTATGAACT GTTGACCACC GTGGAAGAGC TTTATGAACA AAACGGTTAC 
CACGGCTCCA CTGAGAAGTT TTTCAGCCTG GTGGAGAAAT GTGCTGATAA AAGACCAGAT 
GCATCAGTGC TGACACTTAT CTCCTACAGG GCTCAGTCTA TCCAGCCTGC TAAAGAAGGC 
TGGATTCAGA GCCTTCACCG GCTCATGGAG AAATTCTTCA GGAACGAGAC TCGCAGTGTG 
ATAAGAATCA AAGTGCTTCA TATCCTGTCC TTCGTTCTCA GTACCAACCG GCAGCTCTAT 
GAGGATGAGT TGATTGAAAT GGTGGTGATT CCCCAGCTCA GTGGGATTGC TGAAGACCGG 
GACTTGGCAG TTAGAAAACA GGCTACTCAG CTATTAGTAG ACCTCGCTGA GGGCTGCAAC 
ACGCACCACT TCACCAGCCT GCTGGACATC ATTGAACGGG TGTCTAGCCG CTCTCTGGTG 
TGTTCAGGGC CCCTAGAGGT TTCTGAGAGA GACCCCACAG CAGAGTCTCC TATGGAGGAC 
GTCAGGACTG CTATTCTGGG CCTGCTTGAA ATCCTGCAGA GCAAACTTTA CAGTCTGCCA 
GCCAGCCATG CGAGTCGTGT TTATGAGTTG CTTATCAGCC ACCTGCAGCT TCATTACAAG 
AACAAGTACT GTTCAACTAT CGCCTCCACT ATTAGACTAA AGGTGTTTGA CTTCTTCCTC 
ATGATGCGTG CAGACTCTCT TCACCGTCTT GGGGTCCCAA ACAAAGATGG TGTCATGAGA 
TTCAGCCCTT ATTGCTACTG TGATATAGGG GAACCAGAGA AACGTGTTGG TGAAAAGAAG 
CCAGCAGGTT CAACCTCTCC TCCTGCTGGC AGCCCTGCTC CTCCTGCTGC TCCTCCCAGT 
TCAGCAGCCT CAACACGCTC AGCCTACCTT TCCTATTCGC CTGCCTTCAG TGTCCTCCTG 
CAGTGCCTCA AGATGGAGAC GGATTGGAAG GTGCTGAAGC TGGTTCTTGA CAAGCTGCCA 
TGGATGCTGC AGTACAAAGT GTTGCTGCTC ACATCACCAT GCAACTTAGA TCAGCTGTGT 
TCTACACTCT GCTGCATGGT GACAGATCGT CTCATCTCAG AGCGATTGAA AAAGACTCCA 
GAGGGGTTTT CTCGCACAGA TGTTCAGCTG GCAGTGATTC CCGTTCTTAC AGCAATTACC 
TCCTACCACA ACTACTTGGA GCAGTCAAGA CAGAGAGAAC TGGTTCAGTG CCTCGAGACT 
GGGCTCATTT ACCGCTGTGC CAAGCAATGT GTGGTGGCCC TTACAATGTG CACGGTGGAG 
ATGCCTGATA TCATGATCAA GCTTCTTCCT GCTCTTATCG TCAAGCTCAC TCACATCTCT 
GCTACTGTTG CCATGGCATC TCCTATGCTC GAGTTTCTCT CCACCCTCGT GCGCCTTCCC 
CATCTGTATG CCAACTTTGT AGCTGAGCAG TATGTCAGCG TGTTTGCCAT CTCACTACCT 
TACACTAACC CCTCAAAGTT CAACCAGTAC ATTGTGTCCC TGGCCCACCA TGTGATTGCC 
ATGTGGTTCA TACGCTGCAG ACTACCCTTC CGCAAGGACT TTGTTCAGTA TATCACAAAG 
GGTTTGCGTT CCAACGCCCT TCTGCCATTC GATGACAGTC ATGAGCAGAG TCCGTTTCGT 
GCCCGAAGCA CCAGCCTCAA TGAGAGACCC AAGAGCTTGC GGGCAGCAAA AGTGGCAAAG 
GCAGCAGCAG CGGTAGCCAA TAGCAGCAGC TCTCCAGTTA AAGAGCTGAG GGACCTTTCA 
GCCATGGACG CTTTCCGCTC CCGCAGCATC AGCGTCTCCG AACATGCGGT CCGCAGGATG 
CACACCTCAA CCACCACATG CAGTCTGGGC TCTGCCGATG AAAATGCAGT AACTCAGGCC 
GACGAGGGGC TGAAAACTGT CCACCTAGAG CTCACAGAGA CTTGTCTTGA CATGATGGCC 
CGATATGTAT TTTCCAACTT CTCTGCACTT CCCAAGAGGT CTCCCATCGC AGAGTTCCTG 
TTAGCAGGAG GCCGCAGTAT GACCTGGTTG GTGGGCAACA AGTTGGTCAC CATAACAACC 
AGCGGAGGGG TCAGAACACA AGCGCTGCTG GGCCTGGACA TGGCTGATCG CCTGGGAGGA 
GGAGGAGGGG AATTGACCAG GTCAGACCCA TCACTACACA CCCGAATGAC CAAAGAGGCT 
CCAGCCAAAT TGGAGTCACA GTCAAGCCAG CAGCACAACA GAGCTGCACG CATACGAGTT 
CGCTCCATGT CAGGTGGTCA TGCTCTACGT GCCGGTCCAG CTCAGAGTCT CAGTCCTCTG 
GTGTCTCCCT CTGAGGGTGA GTTGACTGCT CCACTCTCTC CCTCCTCTGG TCCTGCTCTG 
GATAGTTTAG GTCCATCCTC TTCCACCTCT GCCACTCCAT CTGTGCCTCC TACTCTGAAA 
GACAACCCCA CCTTGGCTGA ATTTGCCCCA ATGCTCACAC AAGGCTGGGC AGAGATCTTC 
ATACGCAGAC CGTCAGGTAA CACAAGCTGG CTAATGTGTT TGGAGAACCC ACCTAGCCCT 
TTCTCATCTG AGCTTGGCAA CATGCCGCTG CAAGAGCTCT CCAGTGTTCT GATGGCAATG 
GAGGGAGTGA AGGAACCTCC CACTCAGACA GCCAGTGCTC CTGCTAGCAC AGCTGTCCCT 
GCTCCAGCTC CTGTCTCTGA GCCCCTAACC CAAACACACA GCAGTGTTGG AGGAAAGGCA 
AACCTGATTC AGCGCTCCAA CACAGTGGGT GGCTCTCTCT GCTCTCTGGG TATGGGCACT 
GCCCCCCCAG GCCCACCAGC CCCTGGCAGG TTGCACAGGA GCATTTCCTG GGCAGACTCT 
GTCGTGGTGC TGGAGGAGGG ATCAGGAATT GCTACAGCAA ACACGCCGCC TGAATGGCTG 
GGGAGTGCAG AGTTTGAGCC AATGTTGTCT GATCCAATCT TCATAGCTGC TGATAAGTTT 
ACAAAGACTC CTCCTGAAAC ACTCAGCAGG TCCTCCTCCA CCTCAAGTCA GGATGATGAA 
AAGTCAACCC TGGAAGAAGT AAGTGAGGGA GCAATTCCAA TCGATCAGCC CCCTTTTGGA 
CCATCCACTC CAGGAAGCCA GGGGCCTGAC CTTCCCTTCC AGACACACAC TCAGTCCCAG 
GGTCATGGAC TCAACAAGTC CAGCTCCTCT CCTGAGCTCC AGACATTACC TGAAGCTTTT 
GCCAAGGCTG CTATGGAGTC AGAAAGTGCG ATGGTTGACA CGTCACGGCC TAGAGCACCC 
TCAGACGGCA TGCCTCAACC CCAGCAGCCC CAGTTTGCGA AAGAAAAGGT AGAGGGAAAC 
ACTGGAGTGG ACACTAATGT AGCGGGAGGT CCAAGCCCAG CAGGTGAAGG CCAAGCTGTA 
TCGTCATCTC AGAGTGGAGG AGCGGGGATG AGGTTGTCTT TTCCACCAGC ACCAGTACAA 
TCTGCACCCA TCTCCCCCAG TGGAGGCCAC CGGCCCAGAG GTCACACAAT CTCAGTCTCA 
GCCCCCTCCT CCAGGAGGGA GAGGCGGGCG GAGAGGGACG CTTACCACAG TCGACCTGGT 
CCCAGCAACA CTGAGAAAAT CTCTGGACTG AGCCCCAGCT TTGTGTTTCT GCAGCTCTAC 
CACTCTCCTT TCTTTGGGAA TGAAGCCAAC AAGCCACTTC TGCTGCCCAA AACTCAGGTG 
ATTGACCGTG CAGTAAAGGT TCTGGACCAA ATGCCCCCTT ATGACACCCA TAAAATTGGG 
GTGGTGTTTG TAGGAGCTGG TCAGGTTAAC AATGAAGTCG CCATCTTGTC GAACGAGTAC 
GGTTCGAACC GCTACGCCTC TTTCCTCACA GGTCTTGGCA AATTAATCCA TCTGAAAGAC 
TGCGATCCAG ACCAGATTTT TCTTGGAGGG CTTGACCAGT ATGGAGATGA TGGAGAGTTC 
ACCTACTGCT GGCATGACGA CATCATGCAG GCTATCTTTC ACATAGCCAC ACTGATGCCA 
AACAGGGAGA GCGACAAGGG TTGCTGCAAC AAAAAGCGAC ACATTGGCAA CGATTTTGTC 
ATGGTGGTGT ACAATGACTC TGGAGAGGAG TACAAACTGG GCACAATTAA GGGTCAGTTT 
AATTTTGTCG AAGTCATTAT AAAACCACTG GATTATGAAT GTAACCTCGT TACCCTCCAG 
TGCCGCAAAG ACCTCGAGGG ATTAGTAGAC ACAACGGTGG CAAAAATTGT TTCAGATCGA 
AACCTTCCCC TGTTAGTCAG GCAGATGGCT CTGCATGCAA ATATGGCCTC ATTGGTGCAC 
CAGTACAGAG CTAACCCCTC TGACGCATAC GCCTCTAAGT GGCTAGCAAG GTTACGACAC 
ATCAAGAGGA TCAGGACCAG GGCTCAAGAA GCCATCCAGT CTCGCTCAAC TCCCGGGATT 
TCACTGACCC AAGGGCAGAG CAAACCTTTG TCACAGAGCA GTGCTCCAAC AAATGTGGAA 
GCCACCGGAC AACGAAAAAG ACTGGTGTCC ACTGTAGACG ATTTCACAGA TTTTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tsc2 ( XM_004539039.4 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004539096.1 Maylandia zebra TSC complex subunit 2 (tsc2), transcript variant X4, mRNA.

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CloneID	OMa374613
Clone ID Related Accession (Same CDS sequence)	XM_004539039.4
Accession Version	XM_004539039.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5610bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-23
Organism	Maylandia zebra(zebra mbuna)
Product	tuberin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036785.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004539039.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAAAC AACCAAGCAA GGAGAGTCTC AAGGACAAGG TGAAGGGGAT CTTTGGGCTC 
GGTACACAGC GTCCTCCGAA CAAACAGAGT GACACTAAAC CCTCCGAGTT TATCATTACG 
GCTGACATCA TCAAGGAGCT CCACCCAGAT TGCGGTCTGA GCAACCGTGT TCGCACTATG 
AACCACATCT GTGAGCTTGC CAAAATGAAA AAGTTTGAGG AGCATGCAGT GGAGGCTGTA 
TGGAAAAATG TGGAAGACAT GCTGACTCCA GAGCAGCCAC CAGAGGCCAG ACATGCTGTC 
CTGCAGCTGC TCAGGGCCAT TATACAGGGG CAGGGTGAGC GACTCGGCCC TCTGAGAGCC 
TACTTTTTCA AGGTAATCAG AGACTACCAG CCGTGCAATG AAGACCTTTC TGACAGACTG 
GAGGTGTTTA AGGCTTTAAC AGAGAATGGA AAGGATATCA CATACCTGGA AGAGGACATA 
GCACGCTTTG TCCTCCTGTG GATGGATATA GGCCTTACAT CTGACTTTCT CCATGTTCTT 
GTTAATCTGG TGAAATTCAA TAGCTGCTAC TTGGACCAAA ATGTTTCCAT CATGGTCCAG 
AAAATTTGCC TGCTGTGCAA CAGAACGACG TCTTCGACTG ATATTGAGGT GGCTCTGCAA 
GTGCTCGATG CAGTTGTGTG TTACAACTGC CTGCCCTCCG ACTCTTTAAC CGTTTTCATA 
ATCACACTCT GCCGTACGGT CAACGTCAAA GAGTTTTGCG AGTCCTGTTG GAAGTTAATG 
AGAAAGGTTT TGGGGACTCA CCTCGGCCAT AGTGCAATTT ACACCATGTG TCGCATTATG 
GAGGAAAGGG TGTATATGGA GGATGCACCG TTGTTAAGAG GAGCAGTTTT CTTTGTTGGA 
ATGGCGCTTT GGGGCGCACA CAGACTGCCT GCACTCAAAA ATACACCGAC GCTTGTTCTG 
CCATCTTTCT ACAAGGCCAT GTCATGTGCC AACGAGGTGG TCTCCTACGA AATTGTTCTT 
TCAATAACAA GACTCATCAA AAAGTACGGT AAAGAGCTAC AAGTGGTCAC ATGGGACATC 
CTACTCGGCA TCATAGAGCG ACTGTTGCAA CAGATTCAGA CAATAGGCAG TGCGGAACTA 
AAGGCCATTG TTTATGAACT GTTGACCACC GTGGAAGAGC TTTATGAACA AAACGGTTAC 
CACGGCTCCA CTGAGAAGTT TTTCAGCCTG GTGGAGAAAT GTGCTGATAA AAGACCAGAT 
GCATCAGTGC TGACACTTAT CTCCTACAGG GCTCAGTCTA TCCAGCCTGC TAAAGAAGGC 
TGGATTCAGA GCCTTCACCG GCTCATGGAG AAATTCTTCA GGAACGAGAC TCGCAGTGTG 
ATAAGAATCA AAGTGCTTCA TATCCTGTCC TTCGTTCTCA GTACCAACCG GCAGCTCTAT 
GAGGATGAGT TGATTGAAAT GGTGGTGATT CCCCAGCTCA GTGGGATTGC TGAAGACCGG 
GACTTGGCAG TTAGAAAACA GGCTACTCAG CTATTAGTAG ACCTCGCTGA GGGCTGCAAC 
ACGCACCACT TCACCAGCCT GCTGGACATC ATTGAACGGG TGTCTAGCCG CTCTCTGGTG 
TGTTCAGGGC CCCTAGAGGT TTCTGAGAGA GACCCCACAG CAGAGTCTCC TATGGAGGAC 
GTCAGGACTG CTATTCTGGG CCTGCTTGAA ATCCTGCAGA GCAAACTTTA CAGTCTGCCA 
GCCAGCCATG CGAGTCGTGT TTATGAGTTG CTTATCAGCC ACCTGCAGCT TCATTACAAG 
AACAAGTACT GTTCAACTAT CGCCTCCACT ATTAGACTAA AGGTGTTTGA CTTCTTCCTC 
ATGATGCGTG CAGACTCTCT TCACCGTCTT GGGGTCCCAA ACAAAGATGG TGTCATGAGA 
TTCAGCCCTT ATTGCTACTG TGATATAGGG GAACCAGAGA AACGTGTTGG TGAAAAGAAG 
CCAGCAGGTT CAACCTCTCC TCCTGCTGGC AGCCCTGCTC CTCCTGCTGC TCCTCCCAGT 
TCAGCAGCCT CAACACGCTC AGCCTACCTT TCCTATTCGC CTGCCTTCAG TGTCCTCCTG 
CAGTGCCTCA AGATGGAGAC GGATTGGAAG GTGCTGAAGC TGGTTCTTGA CAAGCTGCCA 
TGGATGCTGC AGTACAAAGT GTTGCTGCTC ACATCACCAT GCAACTTAGA TCAGCTGTGT 
TCTACACTCT GCTGCATGGT GACAGATCGT CTCATCTCAG AGCGATTGAA AAAGACTCCA 
GAGGGGTTTT CTCGCACAGA TGTTCAGCTG GCAGTGATTC CCGTTCTTAC AGCAATTACC 
TCCTACCACA ACTACTTGGA GCAGTCAAGA CAGAGAGAAC TGGTTCAGTG CCTCGAGACT 
GGGCTCATTT ACCGCTGTGC CAAGCAATGT GTGGTGGCCC TTACAATGTG CACGGTGGAG 
ATGCCTGATA TCATGATCAA GCTTCTTCCT GCTCTTATCG TCAAGCTCAC TCACATCTCT 
GCTACTGTTG CCATGGCATC TCCTATGCTC GAGTTTCTCT CCACCCTCGT GCGCCTTCCC 
CATCTGTATG CCAACTTTGT AGCTGAGCAG TATGTCAGCG TGTTTGCCAT CTCACTACCT 
TACACTAACC CCTCAAAGTT CAACCAGTAC ATTGTGTCCC TGGCCCACCA TGTGATTGCC 
ATGTGGTTCA TACGCTGCAG ACTACCCTTC CGCAAGGACT TTGTTCAGTA TATCACAAAG 
GGTTTGCGTT CCAACGCCCT TCTGCCATTC GATGACAGTC ATGAGCAGAG TCCGTTTCGT 
GCCCGAAGCA CCAGCCTCAA TGAGAGACCC AAGAGCTTGC GGGCAGCAAA AGTGGCAAAG 
GCAGCAGCAG CGGTAGCCAA TAGCAGCAGC TCTCCAGTTA AAGAGCTGAG GGACCTTTCA 
GCCATGGACG CTTTCCGCTC CCGCAGCATC AGCGTCTCCG AACATGCGGT CCGCAGGATG 
CACACCTCAA CCACCACATG CAGTCTGGGC TCTGCCGATG AAAATGCAGT AACTCAGGCC 
GACGAGGGGC TGAAAACTGT CCACCTAGAG CTCACAGAGA CTTGTCTTGA CATGATGGCC 
CGATATGTAT TTTCCAACTT CTCTGCACTT CCCAAGAGGT CTCCCATCGC AGAGTTCCTG 
TTAGCAGGAG GCCGCAGTAT GACCTGGTTG GTGGGCAACA AGTTGGTCAC CATAACAACC 
AGCGGAGGGG TCAGAACACA AGCGCTGCTG GGCCTGGACA TGGCTGATCG CCTGGGAGGA 
GGAGGAGGGG AATTGACCAG GTCAGACCCA TCACTACACA CCCGAATGAC CAAAGAGGCT 
CCAGCCAAAT TGGAGTCACA GTCAAGCCAG CAGCACAACA GAGCTGCACG CATACGAGTT 
CGCTCCATGT CAGGTGGTCA TGCTCTACGT GCCGGTCCAG CTCAGAGTCT CAGTCCTCTG 
GTGTCTCCCT CTGAGGGTGA GTTGACTGCT CCACTCTCTC CCTCCTCTGG TCCTGCTCTG 
GATAGTTTAG GTCCATCCTC TTCCACCTCT GCCACTCCAT CTGTGCCTCC TACTCTGAAA 
GACAACCCCA CCTTGGCTGA ATTTGCCCCA ATGCTCACAC AAGGCTGGGC AGAGATCTTC 
ATACGCAGAC CGTCAGGTAA CACAAGCTGG CTAATGTGTT TGGAGAACCC ACCTAGCCCT 
TTCTCATCTG AGCTTGGCAA CATGCCGCTG CAAGAGCTCT CCAGTGTTCT GATGGCAATG 
GAGGGAGTGA AGGAACCTCC CACTCAGACA GCCAGTGCTC CTGCTAGCAC AGCTGTCCCT 
GCTCCAGCTC CTGTCTCTGA GCCCCTAACC CAAACACACA GCAGTGTTGG AGGAAAGGCA 
AACCTGATTC AGCGCTCCAA CACAGACTCT GTCGTGGTGC TGGAGGAGGG ATCAGGAATT 
GCTACAGCAA ACACGCCGCC TGAATGGCTG GGGAGTGCAG AGTTTGAGCC AATGTTGTCT 
GATCCAATCT TCATAGCTGC TGATAAGTTT ACAAAGACTC CTCCTGAAAC ACTCAGCAGG 
TCCTCCTCCA CCTCAAGTCA GGATGATGAA AAGTCAACCC TGGAAGAAGT AAGTGAGGGA 
GCAATTCCAA TCGATCAGCC CCCTTTTGGA CCATCCACTC CAGGAAGCCA GGGGCCTGAC 
CTTCCCTTCC AGACACACAC TCAGTCCCAG GGTCATGGAC TCAACAAGTC CAGCTCCTCT 
CCTGAGCTCC AGACATTACC TGAAGCTTTT GCCAAGGCTG CTATGGAGTC AGAAAGTGCG 
ATGGTTGACA CGTCACGGCC TAGAGCACCC TCAGACGGCA TGCCTCAACC CCAGCAGCCC 
CAGTTTGCGA AAGAAAAGGT AGAGGGAAAC ACTGGAGTGG ACACTAATGT AGCGGGAGGT 
CCAAGCCCAG CAGGTGAAGG CCAAGCTGTA TCGTCATCTC AGAGTGGAGG AGCGGGGATG 
AGGTTGTCTT TTCCACCAGC ACCAGTACAA TCTGCACCCA TCTCCCCCAG TGGAGGCCAC 
CGGCCCAGAG GTCACACAAT CTCAGTCTCA GCCCCCTCCT CCAGGAGGGA GAGGCGGGCG 
GAGAGGGACG CTTACCACAG TCGACCTGGT CCCAGCAACA CTGAGAAAAT CTCTGGACTG 
AGCCCCAGCT TTGTGTTTCT GCAGCTCTAC CACTCTCCTT TCTTTGGGAA TGAAGCCAAC 
AAGCCACTTC TGCTGCCCAA AACTCAGGTG ATTGACCGTG CAGTAAAGGT TCTGGACCAA 
ATGCCCCCTT ATGACACCCA TAAAATTGGG GTGGTGTTTG TAGGAGCTGG TCAGGTTAAC 
AATGAAGTCG CCATCTTGTC GAACGAGTAC GGTTCGAACC GCTACGCCTC TTTCCTCACA 
GGTCTTGGCA AATTAATCCA TCTGAAAGAC TGCGATCCAG ACCAGATTTT TCTTGGAGGG 
CTTGACCAGT ATGGAGATGA TGGAGAGTTC ACCTACTGCT GGCATGACGA CATCATGCAG 
GCTATCTTTC ACATAGCCAC ACTGATGCCA AACAGGGAGA GCGACAAGGG TTGCTGCAAC 
AAAAAGCGAC ACATTGGCAA CGATTTTGTC ATGGTGGTGT ACAATGACTC TGGAGAGGAG 
TACAAACTGG GCACAATTAA GGGTCAGTTT AATTTTGTCG AAGTCATTAT AAAACCACTG 
GATTATGAAT GTAACCTCGT TACCCTCCAG TGCCGCAAAG ACCTCGAGGG ATTAGTAGAC 
ACAACGGTGG CAAAAATTGT TTCAGATCGA AACCTTCCCC TGTTAGTCAG GCAGATGGCT 
CTGCATGCAA ATATGGCCTC ATTGGTGCAC CAGTACAGAG CTAACCCCTC TGACGCATAC 
GCCTCTAAGT GGCTAGCAAG GTTACGACAC ATCAAGAGGA TCAGGACCAG GGCTCAAGAA 
GCCATCCAGT CTCGCTCAAC TCCCGGGATT TCACTGACCC AAGGGCAGAG CAAACCTTTG 
TCACAGAGCA GTGCTCCAAC AAATGTGGAA GCCACCGGAC AACGAAAAAG ACTGGTGTCC 
ACTGTAGACG ATTTCACAGA TTTTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004539096.1
CDS	198..5807
Translation	MNKQPSKESLKDKVKGIFGLGTQRPPNKQSDTKPSEFIITADIIKELHPDCGLSNRVRTMNHICELAKMKKFEEHAVEAVWKNVEDMLTPEQPPEARHAVLQLLRAIIQGQGERLGPLRAYFFKVIRDYQPCNEDLSDRLEVFKALTENGKDITYLEEDIARFVLLWMDIGLTSDFLHVLVNLVKFNSCYLDQNVSIMVQKICLLCNRTTSSTDIEVALQVLDAVVCYNCLPSDSLTVFIITLCRTVNVKEFCESCWKLMRKVLGTHLGHSAIYTMCRIMEERVYMEDAPLLRGAVFFVGMALWGAHRLPALKNTPTLVLPSFYKAMSCANEVVSYEIVLSITRLIKKYGKELQVVTWDILLGIIERLLQQIQTIGSAELKAIVYELLTTVEELYEQNGYHGSTEKFFSLVEKCADKRPDASVLTLISYRAQSIQPAKEGWIQSLHRLMEKFFRNETRSVIRIKVLHILSFVLSTNRQLYEDELIEMVVIPQLSGIAEDRDLAVRKQATQLLVDLAEGCNTHHFTSLLDIIERVSSRSLVCSGPLEVSERDPTAESPMEDVRTAILGLLEILQSKLYSLPASHASRVYELLISHLQLHYKNKYCSTIASTIRLKVFDFFLMMRADSLHRLGVPNKDGVMRFSPYCYCDIGEPEKRVGEKKPAGSTSPPAGSPAPPAAPPSSAASTRSAYLSYSPAFSVLLQCLKMETDWKVLKLVLDKLPWMLQYKVLLLTSPCNLDQLCSTLCCMVTDRLISERLKKTPEGFSRTDVQLAVIPVLTAITSYHNYLEQSRQRELVQCLETGLIYRCAKQCVVALTMCTVEMPDIMIKLLPALIVKLTHISATVAMASPMLEFLSTLVRLPHLYANFVAEQYVSVFAISLPYTNPSKFNQYIVSLAHHVIAMWFIRCRLPFRKDFVQYITKGLRSNALLPFDDSHEQSPFRARSTSLNERPKSLRAAKVAKAAAAVANSSSSPVKELRDLSAMDAFRSRSISVSEHAVRRMHTSTTTCSLGSADENAVTQADEGLKTVHLELTETCLDMMARYVFSNFSALPKRSPIAEFLLAGGRSMTWLVGNKLVTITTSGGVRTQALLGLDMADRLGGGGGELTRSDPSLHTRMTKEAPAKLESQSSQQHNRAARIRVRSMSGGHALRAGPAQSLSPLVSPSEGELTAPLSPSSGPALDSLGPSSSTSATPSVPPTLKDNPTLAEFAPMLTQGWAEIFIRRPSGNTSWLMCLENPPSPFSSELGNMPLQELSSVLMAMEGVKEPPTQTASAPASTAVPAPAPVSEPLTQTHSSVGGKANLIQRSNTDSVVVLEEGSGIATANTPPEWLGSAEFEPMLSDPIFIAADKFTKTPPETLSRSSSTSSQDDEKSTLEEVSEGAIPIDQPPFGPSTPGSQGPDLPFQTHTQSQGHGLNKSSSSPELQTLPEAFAKAAMESESAMVDTSRPRAPSDGMPQPQQPQFAKEKVEGNTGVDTNVAGGPSPAGEGQAVSSSQSGGAGMRLSFPPAPVQSAPISPSGGHRPRGHTISVSAPSSRRERRAERDAYHSRPGPSNTEKISGLSPSFVFLQLYHSPFFGNEANKPLLLPKTQVIDRAVKVLDQMPPYDTHKIGVVFVGAGQVNNEVAILSNEYGSNRYASFLTGLGKLIHLKDCDPDQIFLGGLDQYGDDGEFTYCWHDDIMQAIFHIATLMPNRESDKGCCNKKRHIGNDFVMVVYNDSGEEYKLGTIKGQFNFVEVIIKPLDYECNLVTLQCRKDLEGLVDTTVAKIVSDRNLPLLVRQMALHANMASLVHQYRANPSDAYASKWLARLRHIKRIRTRAQEAIQSRSTPGISLTQGQSKPLSQSSAPTNVEATGQRKRLVSTVDDFTDFV

Target ORF information:

RefSeq Version	XM_004539039.4
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra TSC complex subunit 2 (tsc2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004539039.4

ORF Insert Sequence:

ATGAATAAAC AACCAAGCAA GGAGAGTCTC AAGGACAAGG TGAAGGGGAT CTTTGGGCTC 
GGTACACAGC GTCCTCCGAA CAAACAGAGT GACACTAAAC CCTCCGAGTT TATCATTACG 
GCTGACATCA TCAAGGAGCT CCACCCAGAT TGCGGTCTGA GCAACCGTGT TCGCACTATG 
AACCACATCT GTGAGCTTGC CAAAATGAAA AAGTTTGAGG AGCATGCAGT GGAGGCTGTA 
TGGAAAAATG TGGAAGACAT GCTGACTCCA GAGCAGCCAC CAGAGGCCAG ACATGCTGTC 
CTGCAGCTGC TCAGGGCCAT TATACAGGGG CAGGGTGAGC GACTCGGCCC TCTGAGAGCC 
TACTTTTTCA AGGTAATCAG AGACTACCAG CCGTGCAATG AAGACCTTTC TGACAGACTG 
GAGGTGTTTA AGGCTTTAAC AGAGAATGGA AAGGATATCA CATACCTGGA AGAGGACATA 
GCACGCTTTG TCCTCCTGTG GATGGATATA GGCCTTACAT CTGACTTTCT CCATGTTCTT 
GTTAATCTGG TGAAATTCAA TAGCTGCTAC TTGGACCAAA ATGTTTCCAT CATGGTCCAG 
AAAATTTGCC TGCTGTGCAA CAGAACGACG TCTTCGACTG ATATTGAGGT GGCTCTGCAA 
GTGCTCGATG CAGTTGTGTG TTACAACTGC CTGCCCTCCG ACTCTTTAAC CGTTTTCATA 
ATCACACTCT GCCGTACGGT CAACGTCAAA GAGTTTTGCG AGTCCTGTTG GAAGTTAATG 
AGAAAGGTTT TGGGGACTCA CCTCGGCCAT AGTGCAATTT ACACCATGTG TCGCATTATG 
GAGGAAAGGG TGTATATGGA GGATGCACCG TTGTTAAGAG GAGCAGTTTT CTTTGTTGGA 
ATGGCGCTTT GGGGCGCACA CAGACTGCCT GCACTCAAAA ATACACCGAC GCTTGTTCTG 
CCATCTTTCT ACAAGGCCAT GTCATGTGCC AACGAGGTGG TCTCCTACGA AATTGTTCTT 
TCAATAACAA GACTCATCAA AAAGTACGGT AAAGAGCTAC AAGTGGTCAC ATGGGACATC 
CTACTCGGCA TCATAGAGCG ACTGTTGCAA CAGATTCAGA CAATAGGCAG TGCGGAACTA 
AAGGCCATTG TTTATGAACT GTTGACCACC GTGGAAGAGC TTTATGAACA AAACGGTTAC 
CACGGCTCCA CTGAGAAGTT TTTCAGCCTG GTGGAGAAAT GTGCTGATAA AAGACCAGAT 
GCATCAGTGC TGACACTTAT CTCCTACAGG GCTCAGTCTA TCCAGCCTGC TAAAGAAGGC 
TGGATTCAGA GCCTTCACCG GCTCATGGAG AAATTCTTCA GGAACGAGAC TCGCAGTGTG 
ATAAGAATCA AAGTGCTTCA TATCCTGTCC TTCGTTCTCA GTACCAACCG GCAGCTCTAT 
GAGGATGAGT TGATTGAAAT GGTGGTGATT CCCCAGCTCA GTGGGATTGC TGAAGACCGG 
GACTTGGCAG TTAGAAAACA GGCTACTCAG CTATTAGTAG ACCTCGCTGA GGGCTGCAAC 
ACGCACCACT TCACCAGCCT GCTGGACATC ATTGAACGGG TGTCTAGCCG CTCTCTGGTG 
TGTTCAGGGC CCCTAGAGGT TTCTGAGAGA GACCCCACAG CAGAGTCTCC TATGGAGGAC 
GTCAGGACTG CTATTCTGGG CCTGCTTGAA ATCCTGCAGA GCAAACTTTA CAGTCTGCCA 
GCCAGCCATG CGAGTCGTGT TTATGAGTTG CTTATCAGCC ACCTGCAGCT TCATTACAAG 
AACAAGTACT GTTCAACTAT CGCCTCCACT ATTAGACTAA AGGTGTTTGA CTTCTTCCTC 
ATGATGCGTG CAGACTCTCT TCACCGTCTT GGGGTCCCAA ACAAAGATGG TGTCATGAGA 
TTCAGCCCTT ATTGCTACTG TGATATAGGG GAACCAGAGA AACGTGTTGG TGAAAAGAAG 
CCAGCAGGTT CAACCTCTCC TCCTGCTGGC AGCCCTGCTC CTCCTGCTGC TCCTCCCAGT 
TCAGCAGCCT CAACACGCTC AGCCTACCTT TCCTATTCGC CTGCCTTCAG TGTCCTCCTG 
CAGTGCCTCA AGATGGAGAC GGATTGGAAG GTGCTGAAGC TGGTTCTTGA CAAGCTGCCA 
TGGATGCTGC AGTACAAAGT GTTGCTGCTC ACATCACCAT GCAACTTAGA TCAGCTGTGT 
TCTACACTCT GCTGCATGGT GACAGATCGT CTCATCTCAG AGCGATTGAA AAAGACTCCA 
GAGGGGTTTT CTCGCACAGA TGTTCAGCTG GCAGTGATTC CCGTTCTTAC AGCAATTACC 
TCCTACCACA ACTACTTGGA GCAGTCAAGA CAGAGAGAAC TGGTTCAGTG CCTCGAGACT 
GGGCTCATTT ACCGCTGTGC CAAGCAATGT GTGGTGGCCC TTACAATGTG CACGGTGGAG 
ATGCCTGATA TCATGATCAA GCTTCTTCCT GCTCTTATCG TCAAGCTCAC TCACATCTCT 
GCTACTGTTG CCATGGCATC TCCTATGCTC GAGTTTCTCT CCACCCTCGT GCGCCTTCCC 
CATCTGTATG CCAACTTTGT AGCTGAGCAG TATGTCAGCG TGTTTGCCAT CTCACTACCT 
TACACTAACC CCTCAAAGTT CAACCAGTAC ATTGTGTCCC TGGCCCACCA TGTGATTGCC 
ATGTGGTTCA TACGCTGCAG ACTACCCTTC CGCAAGGACT TTGTTCAGTA TATCACAAAG 
GGTTTGCGTT CCAACGCCCT TCTGCCATTC GATGACAGTC ATGAGCAGAG TCCGTTTCGT 
GCCCGAAGCA CCAGCCTCAA TGAGAGACCC AAGAGCTTGC GGGCAGCAAA AGTGGCAAAG 
GCAGCAGCAG CGGTAGCCAA TAGCAGCAGC TCTCCAGTTA AAGAGCTGAG GGACCTTTCA 
GCCATGGACG CTTTCCGCTC CCGCAGCATC AGCGTCTCCG AACATGCGGT CCGCAGGATG 
CACACCTCAA CCACCACATG CAGTCTGGGC TCTGCCGATG AAAATGCAGT AACTCAGGCC 
GACGAGGGGC TGAAAACTGT CCACCTAGAG CTCACAGAGA CTTGTCTTGA CATGATGGCC 
CGATATGTAT TTTCCAACTT CTCTGCACTT CCCAAGAGGT CTCCCATCGC AGAGTTCCTG 
TTAGCAGGAG GCCGCAGTAT GACCTGGTTG GTGGGCAACA AGTTGGTCAC CATAACAACC 
AGCGGAGGGG TCAGAACACA AGCGCTGCTG GGCCTGGACA TGGCTGATCG CCTGGGAGGA 
GGAGGAGGGG AATTGACCAG GTCAGACCCA TCACTACACA CCCGAATGAC CAAAGAGGCT 
CCAGCCAAAT TGGAGTCACA GTCAAGCCAG CAGCACAACA GAGCTGCACG CATACGAGTT 
CGCTCCATGT CAGGTGGTCA TGCTCTACGT GCCGGTCCAG CTCAGAGTCT CAGTCCTCTG 
GTGTCTCCCT CTGAGGGTGA GTTGACTGCT CCACTCTCTC CCTCCTCTGG TCCTGCTCTG 
GATAGTTTAG GTCCATCCTC TTCCACCTCT GCCACTCCAT CTGTGCCTCC TACTCTGAAA 
GACAACCCCA CCTTGGCTGA ATTTGCCCCA ATGCTCACAC AAGGCTGGGC AGAGATCTTC 
ATACGCAGAC CGTCAGGTAA CACAAGCTGG CTAATGTGTT TGGAGAACCC ACCTAGCCCT 
TTCTCATCTG AGCTTGGCAA CATGCCGCTG CAAGAGCTCT CCAGTGTTCT GATGGCAATG 
GAGGGAGTGA AGGAACCTCC CACTCAGACA GCCAGTGCTC CTGCTAGCAC AGCTGTCCCT 
GCTCCAGCTC CTGTCTCTGA GCCCCTAACC CAAACACACA GCAGTGTTGG AGGAAAGGCA 
AACCTGATTC AGCGCTCCAA CACAGACTCT GTCGTGGTGC TGGAGGAGGG ATCAGGAATT 
GCTACAGCAA ACACGCCGCC TGAATGGCTG GGGAGTGCAG AGTTTGAGCC AATGTTGTCT 
GATCCAATCT TCATAGCTGC TGATAAGTTT ACAAAGACTC CTCCTGAAAC ACTCAGCAGG 
TCCTCCTCCA CCTCAAGTCA GGATGATGAA AAGTCAACCC TGGAAGAAGT AAGTGAGGGA 
GCAATTCCAA TCGATCAGCC CCCTTTTGGA CCATCCACTC CAGGAAGCCA GGGGCCTGAC 
CTTCCCTTCC AGACACACAC TCAGTCCCAG GGTCATGGAC TCAACAAGTC CAGCTCCTCT 
CCTGAGCTCC AGACATTACC TGAAGCTTTT GCCAAGGCTG CTATGGAGTC AGAAAGTGCG 
ATGGTTGACA CGTCACGGCC TAGAGCACCC TCAGACGGCA TGCCTCAACC CCAGCAGCCC 
CAGTTTGCGA AAGAAAAGGT AGAGGGAAAC ACTGGAGTGG ACACTAATGT AGCGGGAGGT 
CCAAGCCCAG CAGGTGAAGG CCAAGCTGTA TCGTCATCTC AGAGTGGAGG AGCGGGGATG 
AGGTTGTCTT TTCCACCAGC ACCAGTACAA TCTGCACCCA TCTCCCCCAG TGGAGGCCAC 
CGGCCCAGAG GTCACACAAT CTCAGTCTCA GCCCCCTCCT CCAGGAGGGA GAGGCGGGCG 
GAGAGGGACG CTTACCACAG TCGACCTGGT CCCAGCAACA CTGAGAAAAT CTCTGGACTG 
AGCCCCAGCT TTGTGTTTCT GCAGCTCTAC CACTCTCCTT TCTTTGGGAA TGAAGCCAAC 
AAGCCACTTC TGCTGCCCAA AACTCAGGTG ATTGACCGTG CAGTAAAGGT TCTGGACCAA 
ATGCCCCCTT ATGACACCCA TAAAATTGGG GTGGTGTTTG TAGGAGCTGG TCAGGTTAAC 
AATGAAGTCG CCATCTTGTC GAACGAGTAC GGTTCGAACC GCTACGCCTC TTTCCTCACA 
GGTCTTGGCA AATTAATCCA TCTGAAAGAC TGCGATCCAG ACCAGATTTT TCTTGGAGGG 
CTTGACCAGT ATGGAGATGA TGGAGAGTTC ACCTACTGCT GGCATGACGA CATCATGCAG 
GCTATCTTTC ACATAGCCAC ACTGATGCCA AACAGGGAGA GCGACAAGGG TTGCTGCAAC 
AAAAAGCGAC ACATTGGCAA CGATTTTGTC ATGGTGGTGT ACAATGACTC TGGAGAGGAG 
TACAAACTGG GCACAATTAA GGGTCAGTTT AATTTTGTCG AAGTCATTAT AAAACCACTG 
GATTATGAAT GTAACCTCGT TACCCTCCAG TGCCGCAAAG ACCTCGAGGG ATTAGTAGAC 
ACAACGGTGG CAAAAATTGT TTCAGATCGA AACCTTCCCC TGTTAGTCAG GCAGATGGCT 
CTGCATGCAA ATATGGCCTC ATTGGTGCAC CAGTACAGAG CTAACCCCTC TGACGCATAC 
GCCTCTAAGT GGCTAGCAAG GTTACGACAC ATCAAGAGGA TCAGGACCAG GGCTCAAGAA 
GCCATCCAGT CTCGCTCAAC TCCCGGGATT TCACTGACCC AAGGGCAGAG CAAACCTTTG 
TCACAGAGCA GTGCTCCAAC AAATGTGGAA GCCACCGGAC AACGAAAAAG ACTGGTGTCC 
ACTGTAGACG ATTTCACAGA TTTTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tsc2 ( XM_004539040.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004539097.2 Maylandia zebra TSC complex subunit 2 (tsc2), transcript variant X5, mRNA.

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CloneID	OMa374614
Clone ID Related Accession (Same CDS sequence)	XM_004539040.3
Accession Version	XM_004539040.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-23
Organism	Maylandia zebra(zebra mbuna)
Product	tuberin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036785.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004539040.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAAAC AACCAAGCAA GGAGAGTCTC AAGGACAAGG TGAAGGGGAT CTTTGGGCTC 
GGTACACAGC GTCCTCCGAA CAAACAGAGT GACACTAAAC CCTCCGAGTT TATCATTACG 
GCTGACATCA TCAAGGAGCT CCACCCAGAT TGCGGTCTGA GCAACCGTGT TCGCACTATG 
AACCACATCT GTGAGCTTGC CAAAATGAAA AAGTTTGAGG AGCATGCAGT GGAGGCTGTA 
TGGAAAAATG TGGAAGACAT GCTGACTCCA GAGCAGCCAC CAGAGGCCAG ACATGCTGTC 
CTGCAGCTGC TCAGGGCCAT TATACAGGGG CAGGGTGAGC GACTCGGCCC TCTGAGAGCC 
TACTTTTTCA AGGTAATCAG AGACTACCAG CCGTGCAATG AAGACCTTTC TGACAGACTG 
GAGGTGTTTA AGGCTTTAAC AGAGAATGGA AAGGATATCA CATACCTGGA AGAGGACATA 
GCACGCTTTG TCCTCCTGTG GATGGATATA GGCCTTACAT CTGACTTTCT CCATGTTCTT 
GTTAATCTGG TGAAATTCAA TAGCTGCTAC TTGGACCAAA ATGTTTCCAT CATGGTCCAG 
AAAATTTGCC TGCTGTGCAA CAGAACGACG TCTTCGACTG ATATTGAGGT GGCTCTGCAA 
GTGCTCGATG CAGTTGTGTG TTACAACTGC CTGCCCTCCG ACTCTTTAAC CGTTTTCATA 
ATCACACTCT GCCGTACGGT CAACGTCAAA GAGTTTTGCG AGTCCTGTTG GAAGTTAATG 
AGAAAGGTTT TGGGGACTCA CCTCGGCCAT AGTGCAATTT ACACCATGTG TCGCATTATG 
GAGGAAAGGG TGTATATGGA GGATGCACCG TTGTTAAGAG GAGCAGTTTT CTTTGTTGGA 
ATGGCGCTTT GGGGCGCACA CAGACTGCCT GCACTCAAAA ATACACCGAC GCTTGTTCTG 
CCATCTTTCT ACAAGGCCAT GTCATGTGCC AACGAGGTGG TCTCCTACGA AATTGTTCTT 
TCAATAACAA GACTCATCAA AAAGTACGGT AAAGAGCTAC AAGTGGTCAC ATGGGACATC 
CTACTCGGCA TCATAGAGCG ACTGTTGCAA CAGATTCAGA CAATAGGCAG TGCGGAACTA 
AAGGCCATTG TTTATGAACT GTTGACCACC GTGGAAGAGC TTTATGAACA AAACGGTTAC 
CACGGCTCCA CTGAGAAGTT TTTCAGCCTG GTGGAGAAAT GTGCTGATAA AAGACCAGAT 
GCATCAGTGC TGACACTTAT CTCCTACAGG GCTCAGTCTA TCCAGCCTGC TAAAGAAGGC 
TGGATTCAGA GCCTTCACCG GCTCATGGAG AAATTCTTCA GGAACGAGAC TCGCAGTGTG 
ATAAGAATCA AAGTGCTTCA TATCCTGTCC TTCGTTCTCA GTACCAACCG GCAGCTCTAT 
GAGGATGAGT TGATTGAAAT GGTGGTGATT CCCCAGCTCA GTGGGATTGC TGAAGACCGG 
GACTTGGCAG TTAGAAAACA GGCTACTCAG CTATTAGTAG ACCTCGCTGA GGGCTGCAAC 
ACGCACCACT TCACCAGCCT GCTGGACATC ATTGAACGGG TGTCTAGCCG CTCTCTGGTG 
TGTTCAGGGC CCCTAGAGGT TTCTGAGAGA GACCCCACAG CAGAGTCTCC TATGGAGGAC 
GTCAGGACTG CTATTCTGGG CCTGCTTGAA ATCCTGCAGA GCAAACTTTA CAGTCTGCCA 
GCCAGCCATG CGAGTCGTGT TTATGAGTTG CTTATCAGCC ACCTGCAGCT TCATTACAAG 
AACAAGTACT GTTCAACTAT CGCCTCCACT ATTAGACTAA AGGTGTTTGA CTTCTTCCTC 
ATGATGCGTG CAGACTCTCT TCACCGTCTT GGGGTCCCAA ACAAAGATGG TGTCATGAGA 
TTCAGCCCTT ATTGCTACTG TGATATAGGG GAACCAGAGA AACGTGTTGG TGAAAAGAAG 
CCAGCAGGTT CAACCTCTCC TCCTGCTGGC AGCCCTGCTC CTCCTGCTGC TCCTCCCAGT 
TCAGCAGCCT CAACACGCTC AGCCTACCTT TCCTATTCGC CTGCCTTCAG TGTCCTCCTG 
CAGTGCCTCA AGATGGAGAC GGATTGGAAG GTGCTGAAGC TGGTTCTTGA CAAGCTGCCA 
TGGATGCTGC AGTACAAAGT GTTGCTGCTC ACATCACCAT GCAACTTAGA TCAGCTGTGT 
TCTACACTCT GCTGCATGGT GACAGATCGT CTCATCTCAG AGCGATTGAA AAAGACTCCA 
GAGGGGTTTT CTCGCACAGA TGTTCAGCTG GCAGTGATTC CCGTTCTTAC AGCAATTACC 
TCCTACCACA ACTACTTGGA GCAGTCAAGA CAGAGAGAAC TGGTTCAGTG CCTCGAGACT 
GGGCTCATTT ACCGCTGTGC CAAGCAATGT GTGGTGGCCC TTACAATGTG CACGGTGGAG 
ATGCCTGATA TCATGATCAA GCTTCTTCCT GCTCTTATCG TCAAGCTCAC TCACATCTCT 
GCTACTGTTG CCATGGCATC TCCTATGCTC GAGTTTCTCT CCACCCTCGT GCGCCTTCCC 
CATCTGTATG CCAACTTTGT AGCTGAGCAG TATGTCAGCG TGTTTGCCAT CTCACTACCT 
TACACTAACC CCTCAAAGTT CAACCAGTAC ATTGTGTCCC TGGCCCACCA TGTGATTGCC 
ATGTGGTTCA TACGCTGCAG ACTACCCTTC CGCAAGGACT TTGTTCAGTA TATCACAAAG 
GGTTTGCGTT CCAACGCCCT TCTGCCATTC GATGACAGTC ATGAGCAGAG TCCGTTTCGT 
GCCCGAAGCA CCAGCCTCAA TGAGAGACCC AAGAGGATGC ACACCTCAAC CACCACATGC 
AGTCTGGGCT CTGCCGATGA AAATGCAGTA ACTCAGGCCG ACGAGGGGCT GAAAACTGTC 
CACCTAGAGC TCACAGAGAC TTGTCTTGAC ATGATGGCCC GATATGTATT TTCCAACTTC 
TCTGCACTTC CCAAGAGGTC TCCCATCGCA GAGTTCCTGT TAGCAGGAGG CCGCAGTATG 
ACCTGGTTGG TGGGCAACAA GTTGGTCACC ATAACAACCA GCGGAGGGGT CAGAACACAA 
GCGCTGCTGG GCCTGGACAT GGCTGATCGC CTGGGAGGAG GAGGAGGGGA ATTGACCAGG 
TCAGACCCAT CACTACACAC CCGAATGACC AAAGAGGCTC CAGCCAAATT GGAGTCACAG 
TCAAGCCAGC AGCACAACAG AGCTGCACGC ATACGAGTTC GCTCCATGTC AGGTGGTCAT 
GCTCTACGTG CCGGTCCAGC TCAGAGTCTC AGTCCTCTGG TGTCTCCCTC TGAGGGTGAG 
TTGACTGCTC CACTCTCTCC CTCCTCTGGT CCTGCTCTGG ATAGTTTAGG TCCATCCTCT 
TCCACCTCTG CCACTCCATC TGTGCCTCCT ACTCTGAAAG ACAACCCCAC CTTGGCTGAA 
TTTGCCCCAA TGCTCACACA AGGCTGGGCA GAGATCTTCA TACGCAGACC GTCAGGTAAC 
ACAAGCTGGC TAATGTGTTT GGAGAACCCA CCTAGCCCTT TCTCATCTGA GCTTGGCAAC 
ATGCCGCTGC AAGAGCTCTC CAGTGTTCTG ATGGCAATGG AGGGAGTGAA GGAACCTCCC 
ACTCAGACAG CCAGTGCTCC TGCTAGCACA GCTGTCCCTG CTCCAGCTCC TGTCTCTGAG 
CCCCTAACCC AAACACACAG CAGTGTTGGA GGAAAGGCAA ACCTGATTCA GCGCTCCAAC 
ACAGTGGGTG GCTCTCTCTG CTCTCTGGGT ATGGGCACTG CCCCCCCAGG CCCACCAGCC 
CCTGGCAGGT TGCACAGGAG CATTTCCTGG GCAGACTCTG TCGTGGTGCT GGAGGAGGGA 
TCAGGAATTG CTACAGCAAA CACGCCGCCT GAATGGCTGG GGAGTGCAGA GTTTGAGCCA 
ATGTTGTCTG ATCCAATCTT CATAGCTGCT GATAAGTTTA CAAAGACTCC TCCTGAAACA 
CTCAGCAGGT CCTCCTCCAC CTCAAGTCAG GATGATGAAA AGTCAACCCT GGAAGAAGTA 
AGTGAGGGAG CAATTCCAAT CGATCAGCCC CCTTTTGGAC CATCCACTCC AGGAAGCCAG 
GGGCCTGACC TTCCCTTCCA GACACACACT CAGTCCCAGG GTCATGGACT CAACAAGTCC 
AGCTCCTCTC CTGAGCTCCA GACATTACCT GAAGCTTTTG CCAAGGCTGC TATGGAGTCA 
GAAAGTGCGA TGGTTGACAC GTCACGGCCT AGAGCACCCT CAGACGGCAT GCCTCAACCC 
CAGCAGCCCC AGTTTGCGAA AGAAAAGGTA GAGGGAAACA CTGGAGTGGA CACTAATGTA 
GCGGGAGGTC CAAGCCCAGC AGGTGAAGGC CAAGCTGTAT CGTCATCTCA GAGTGGAGGA 
GCGGGGATGA GGTTGTCTTT TCCACCAGCA CCAGTACAAT CTGCACCCAT CTCCCCCAGT 
GGAGGCCACC GGCCCAGAGG TCACACAATC TCAGTCTCAG CCCCCTCCTC CAGGAGGGAG 
AGGCGGGCGG AGAGGGACGC TTACCACAGT CGACCTGGTC CCAGCAACAC TGAGAAAATC 
TCTGGACTGA GCCCCAGCTT TGTGTTTCTG CAGCTCTACC ACTCTCCTTT CTTTGGGAAT 
GAAGCCAACA AGCCACTTCT GCTGCCCAAA ACTCAGGTGA TTGACCGTGC AGTAAAGGTT 
CTGGACCAAA TGCCCCCTTA TGACACCCAT AAAATTGGGG TGGTGTTTGT AGGAGCTGGT 
CAGGTTAACA ATGAAGTCGC CATCTTGTCG AACGAGTACG GTTCGAACCG CTACGCCTCT 
TTCCTCACAG GTCTTGGCAA ATTAATCCAT CTGAAAGACT GCGATCCAGA CCAGATTTTT 
CTTGGAGGGC TTGACCAGTA TGGAGATGAT GGAGAGTTCA CCTACTGCTG GCATGACGAC 
ATCATGCAGG CTATCTTTCA CATAGCCACA CTGATGCCAA ACAGGGAGAG CGACAAGGGT 
TGCTGCAACA AAAAGCGACA CATTGGCAAC GATTTTGTCA TGGTGGTGTA CAATGACTCT 
GGAGAGGAGT ACAAACTGGG CACAATTAAG GGTCAGTTTA ATTTTGTCGA AGTCATTATA 
AAACCACTGG ATTATGAATG TAACCTCGTT ACCCTCCAGT GCCGCAAAGA CCTCGAGGGA 
TTAGTAGACA CAACGGTGGC AAAAATTGTT TCAGATCGAA ACCTTCCCCT GTTAGTCAGG 
CAGATGGCTC TGCATGCAAA TATGGCCTCA TTGGTGCACC AGTACAGAGC TAACCCCTCT 
GACGCATACG CCTCTAAGTG GCTAGCAAGG TTACGACACA TCAAGAGGAT CAGGACCAGG 
GCTCAAGAAG CCATCCAGTC TCGCTCAACT CCCGGGATTT CACTGACCCA AGGGCAGAGC 
AAACCTTTGT CACAGAGCAG TGCTCCAACA AATGTGGAAG CCACCGGACA ACGAAAAAGA 
CTGGTGTCCA CTGTAGACGA TTTCACAGAT TTTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004539097.2
CDS	198..5756
Translation	MNKQPSKESLKDKVKGIFGLGTQRPPNKQSDTKPSEFIITADIIKELHPDCGLSNRVRTMNHICELAKMKKFEEHAVEAVWKNVEDMLTPEQPPEARHAVLQLLRAIIQGQGERLGPLRAYFFKVIRDYQPCNEDLSDRLEVFKALTENGKDITYLEEDIARFVLLWMDIGLTSDFLHVLVNLVKFNSCYLDQNVSIMVQKICLLCNRTTSSTDIEVALQVLDAVVCYNCLPSDSLTVFIITLCRTVNVKEFCESCWKLMRKVLGTHLGHSAIYTMCRIMEERVYMEDAPLLRGAVFFVGMALWGAHRLPALKNTPTLVLPSFYKAMSCANEVVSYEIVLSITRLIKKYGKELQVVTWDILLGIIERLLQQIQTIGSAELKAIVYELLTTVEELYEQNGYHGSTEKFFSLVEKCADKRPDASVLTLISYRAQSIQPAKEGWIQSLHRLMEKFFRNETRSVIRIKVLHILSFVLSTNRQLYEDELIEMVVIPQLSGIAEDRDLAVRKQATQLLVDLAEGCNTHHFTSLLDIIERVSSRSLVCSGPLEVSERDPTAESPMEDVRTAILGLLEILQSKLYSLPASHASRVYELLISHLQLHYKNKYCSTIASTIRLKVFDFFLMMRADSLHRLGVPNKDGVMRFSPYCYCDIGEPEKRVGEKKPAGSTSPPAGSPAPPAAPPSSAASTRSAYLSYSPAFSVLLQCLKMETDWKVLKLVLDKLPWMLQYKVLLLTSPCNLDQLCSTLCCMVTDRLISERLKKTPEGFSRTDVQLAVIPVLTAITSYHNYLEQSRQRELVQCLETGLIYRCAKQCVVALTMCTVEMPDIMIKLLPALIVKLTHISATVAMASPMLEFLSTLVRLPHLYANFVAEQYVSVFAISLPYTNPSKFNQYIVSLAHHVIAMWFIRCRLPFRKDFVQYITKGLRSNALLPFDDSHEQSPFRARSTSLNERPKRMHTSTTTCSLGSADENAVTQADEGLKTVHLELTETCLDMMARYVFSNFSALPKRSPIAEFLLAGGRSMTWLVGNKLVTITTSGGVRTQALLGLDMADRLGGGGGELTRSDPSLHTRMTKEAPAKLESQSSQQHNRAARIRVRSMSGGHALRAGPAQSLSPLVSPSEGELTAPLSPSSGPALDSLGPSSSTSATPSVPPTLKDNPTLAEFAPMLTQGWAEIFIRRPSGNTSWLMCLENPPSPFSSELGNMPLQELSSVLMAMEGVKEPPTQTASAPASTAVPAPAPVSEPLTQTHSSVGGKANLIQRSNTVGGSLCSLGMGTAPPGPPAPGRLHRSISWADSVVVLEEGSGIATANTPPEWLGSAEFEPMLSDPIFIAADKFTKTPPETLSRSSSTSSQDDEKSTLEEVSEGAIPIDQPPFGPSTPGSQGPDLPFQTHTQSQGHGLNKSSSSPELQTLPEAFAKAAMESESAMVDTSRPRAPSDGMPQPQQPQFAKEKVEGNTGVDTNVAGGPSPAGEGQAVSSSQSGGAGMRLSFPPAPVQSAPISPSGGHRPRGHTISVSAPSSRRERRAERDAYHSRPGPSNTEKISGLSPSFVFLQLYHSPFFGNEANKPLLLPKTQVIDRAVKVLDQMPPYDTHKIGVVFVGAGQVNNEVAILSNEYGSNRYASFLTGLGKLIHLKDCDPDQIFLGGLDQYGDDGEFTYCWHDDIMQAIFHIATLMPNRESDKGCCNKKRHIGNDFVMVVYNDSGEEYKLGTIKGQFNFVEVIIKPLDYECNLVTLQCRKDLEGLVDTTVAKIVSDRNLPLLVRQMALHANMASLVHQYRANPSDAYASKWLARLRHIKRIRTRAQEAIQSRSTPGISLTQGQSKPLSQSSAPTNVEATGQRKRLVSTVDDFTDFV

Target ORF information:

RefSeq Version	XM_004539040.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra TSC complex subunit 2 (tsc2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004539040.3

ORF Insert Sequence:

ATGAATAAAC AACCAAGCAA GGAGAGTCTC AAGGACAAGG TGAAGGGGAT CTTTGGGCTC 
GGTACACAGC GTCCTCCGAA CAAACAGAGT GACACTAAAC CCTCCGAGTT TATCATTACG 
GCTGACATCA TCAAGGAGCT CCACCCAGAT TGCGGTCTGA GCAACCGTGT TCGCACTATG 
AACCACATCT GTGAGCTTGC CAAAATGAAA AAGTTTGAGG AGCATGCAGT GGAGGCTGTA 
TGGAAAAATG TGGAAGACAT GCTGACTCCA GAGCAGCCAC CAGAGGCCAG ACATGCTGTC 
CTGCAGCTGC TCAGGGCCAT TATACAGGGG CAGGGTGAGC GACTCGGCCC TCTGAGAGCC 
TACTTTTTCA AGGTAATCAG AGACTACCAG CCGTGCAATG AAGACCTTTC TGACAGACTG 
GAGGTGTTTA AGGCTTTAAC AGAGAATGGA AAGGATATCA CATACCTGGA AGAGGACATA 
GCACGCTTTG TCCTCCTGTG GATGGATATA GGCCTTACAT CTGACTTTCT CCATGTTCTT 
GTTAATCTGG TGAAATTCAA TAGCTGCTAC TTGGACCAAA ATGTTTCCAT CATGGTCCAG 
AAAATTTGCC TGCTGTGCAA CAGAACGACG TCTTCGACTG ATATTGAGGT GGCTCTGCAA 
GTGCTCGATG CAGTTGTGTG TTACAACTGC CTGCCCTCCG ACTCTTTAAC CGTTTTCATA 
ATCACACTCT GCCGTACGGT CAACGTCAAA GAGTTTTGCG AGTCCTGTTG GAAGTTAATG 
AGAAAGGTTT TGGGGACTCA CCTCGGCCAT AGTGCAATTT ACACCATGTG TCGCATTATG 
GAGGAAAGGG TGTATATGGA GGATGCACCG TTGTTAAGAG GAGCAGTTTT CTTTGTTGGA 
ATGGCGCTTT GGGGCGCACA CAGACTGCCT GCACTCAAAA ATACACCGAC GCTTGTTCTG 
CCATCTTTCT ACAAGGCCAT GTCATGTGCC AACGAGGTGG TCTCCTACGA AATTGTTCTT 
TCAATAACAA GACTCATCAA AAAGTACGGT AAAGAGCTAC AAGTGGTCAC ATGGGACATC 
CTACTCGGCA TCATAGAGCG ACTGTTGCAA CAGATTCAGA CAATAGGCAG TGCGGAACTA 
AAGGCCATTG TTTATGAACT GTTGACCACC GTGGAAGAGC TTTATGAACA AAACGGTTAC 
CACGGCTCCA CTGAGAAGTT TTTCAGCCTG GTGGAGAAAT GTGCTGATAA AAGACCAGAT 
GCATCAGTGC TGACACTTAT CTCCTACAGG GCTCAGTCTA TCCAGCCTGC TAAAGAAGGC 
TGGATTCAGA GCCTTCACCG GCTCATGGAG AAATTCTTCA GGAACGAGAC TCGCAGTGTG 
ATAAGAATCA AAGTGCTTCA TATCCTGTCC TTCGTTCTCA GTACCAACCG GCAGCTCTAT 
GAGGATGAGT TGATTGAAAT GGTGGTGATT CCCCAGCTCA GTGGGATTGC TGAAGACCGG 
GACTTGGCAG TTAGAAAACA GGCTACTCAG CTATTAGTAG ACCTCGCTGA GGGCTGCAAC 
ACGCACCACT TCACCAGCCT GCTGGACATC ATTGAACGGG TGTCTAGCCG CTCTCTGGTG 
TGTTCAGGGC CCCTAGAGGT TTCTGAGAGA GACCCCACAG CAGAGTCTCC TATGGAGGAC 
GTCAGGACTG CTATTCTGGG CCTGCTTGAA ATCCTGCAGA GCAAACTTTA CAGTCTGCCA 
GCCAGCCATG CGAGTCGTGT TTATGAGTTG CTTATCAGCC ACCTGCAGCT TCATTACAAG 
AACAAGTACT GTTCAACTAT CGCCTCCACT ATTAGACTAA AGGTGTTTGA CTTCTTCCTC 
ATGATGCGTG CAGACTCTCT TCACCGTCTT GGGGTCCCAA ACAAAGATGG TGTCATGAGA 
TTCAGCCCTT ATTGCTACTG TGATATAGGG GAACCAGAGA AACGTGTTGG TGAAAAGAAG 
CCAGCAGGTT CAACCTCTCC TCCTGCTGGC AGCCCTGCTC CTCCTGCTGC TCCTCCCAGT 
TCAGCAGCCT CAACACGCTC AGCCTACCTT TCCTATTCGC CTGCCTTCAG TGTCCTCCTG 
CAGTGCCTCA AGATGGAGAC GGATTGGAAG GTGCTGAAGC TGGTTCTTGA CAAGCTGCCA 
TGGATGCTGC AGTACAAAGT GTTGCTGCTC ACATCACCAT GCAACTTAGA TCAGCTGTGT 
TCTACACTCT GCTGCATGGT GACAGATCGT CTCATCTCAG AGCGATTGAA AAAGACTCCA 
GAGGGGTTTT CTCGCACAGA TGTTCAGCTG GCAGTGATTC CCGTTCTTAC AGCAATTACC 
TCCTACCACA ACTACTTGGA GCAGTCAAGA CAGAGAGAAC TGGTTCAGTG CCTCGAGACT 
GGGCTCATTT ACCGCTGTGC CAAGCAATGT GTGGTGGCCC TTACAATGTG CACGGTGGAG 
ATGCCTGATA TCATGATCAA GCTTCTTCCT GCTCTTATCG TCAAGCTCAC TCACATCTCT 
GCTACTGTTG CCATGGCATC TCCTATGCTC GAGTTTCTCT CCACCCTCGT GCGCCTTCCC 
CATCTGTATG CCAACTTTGT AGCTGAGCAG TATGTCAGCG TGTTTGCCAT CTCACTACCT 
TACACTAACC CCTCAAAGTT CAACCAGTAC ATTGTGTCCC TGGCCCACCA TGTGATTGCC 
ATGTGGTTCA TACGCTGCAG ACTACCCTTC CGCAAGGACT TTGTTCAGTA TATCACAAAG 
GGTTTGCGTT CCAACGCCCT TCTGCCATTC GATGACAGTC ATGAGCAGAG TCCGTTTCGT 
GCCCGAAGCA CCAGCCTCAA TGAGAGACCC AAGAGGATGC ACACCTCAAC CACCACATGC 
AGTCTGGGCT CTGCCGATGA AAATGCAGTA ACTCAGGCCG ACGAGGGGCT GAAAACTGTC 
CACCTAGAGC TCACAGAGAC TTGTCTTGAC ATGATGGCCC GATATGTATT TTCCAACTTC 
TCTGCACTTC CCAAGAGGTC TCCCATCGCA GAGTTCCTGT TAGCAGGAGG CCGCAGTATG 
ACCTGGTTGG TGGGCAACAA GTTGGTCACC ATAACAACCA GCGGAGGGGT CAGAACACAA 
GCGCTGCTGG GCCTGGACAT GGCTGATCGC CTGGGAGGAG GAGGAGGGGA ATTGACCAGG 
TCAGACCCAT CACTACACAC CCGAATGACC AAAGAGGCTC CAGCCAAATT GGAGTCACAG 
TCAAGCCAGC AGCACAACAG AGCTGCACGC ATACGAGTTC GCTCCATGTC AGGTGGTCAT 
GCTCTACGTG CCGGTCCAGC TCAGAGTCTC AGTCCTCTGG TGTCTCCCTC TGAGGGTGAG 
TTGACTGCTC CACTCTCTCC CTCCTCTGGT CCTGCTCTGG ATAGTTTAGG TCCATCCTCT 
TCCACCTCTG CCACTCCATC TGTGCCTCCT ACTCTGAAAG ACAACCCCAC CTTGGCTGAA 
TTTGCCCCAA TGCTCACACA AGGCTGGGCA GAGATCTTCA TACGCAGACC GTCAGGTAAC 
ACAAGCTGGC TAATGTGTTT GGAGAACCCA CCTAGCCCTT TCTCATCTGA GCTTGGCAAC 
ATGCCGCTGC AAGAGCTCTC CAGTGTTCTG ATGGCAATGG AGGGAGTGAA GGAACCTCCC 
ACTCAGACAG CCAGTGCTCC TGCTAGCACA GCTGTCCCTG CTCCAGCTCC TGTCTCTGAG 
CCCCTAACCC AAACACACAG CAGTGTTGGA GGAAAGGCAA ACCTGATTCA GCGCTCCAAC 
ACAGTGGGTG GCTCTCTCTG CTCTCTGGGT ATGGGCACTG CCCCCCCAGG CCCACCAGCC 
CCTGGCAGGT TGCACAGGAG CATTTCCTGG GCAGACTCTG TCGTGGTGCT GGAGGAGGGA 
TCAGGAATTG CTACAGCAAA CACGCCGCCT GAATGGCTGG GGAGTGCAGA GTTTGAGCCA 
ATGTTGTCTG ATCCAATCTT CATAGCTGCT GATAAGTTTA CAAAGACTCC TCCTGAAACA 
CTCAGCAGGT CCTCCTCCAC CTCAAGTCAG GATGATGAAA AGTCAACCCT GGAAGAAGTA 
AGTGAGGGAG CAATTCCAAT CGATCAGCCC CCTTTTGGAC CATCCACTCC AGGAAGCCAG 
GGGCCTGACC TTCCCTTCCA GACACACACT CAGTCCCAGG GTCATGGACT CAACAAGTCC 
AGCTCCTCTC CTGAGCTCCA GACATTACCT GAAGCTTTTG CCAAGGCTGC TATGGAGTCA 
GAAAGTGCGA TGGTTGACAC GTCACGGCCT AGAGCACCCT CAGACGGCAT GCCTCAACCC 
CAGCAGCCCC AGTTTGCGAA AGAAAAGGTA GAGGGAAACA CTGGAGTGGA CACTAATGTA 
GCGGGAGGTC CAAGCCCAGC AGGTGAAGGC CAAGCTGTAT CGTCATCTCA GAGTGGAGGA 
GCGGGGATGA GGTTGTCTTT TCCACCAGCA CCAGTACAAT CTGCACCCAT CTCCCCCAGT 
GGAGGCCACC GGCCCAGAGG TCACACAATC TCAGTCTCAG CCCCCTCCTC CAGGAGGGAG 
AGGCGGGCGG AGAGGGACGC TTACCACAGT CGACCTGGTC CCAGCAACAC TGAGAAAATC 
TCTGGACTGA GCCCCAGCTT TGTGTTTCTG CAGCTCTACC ACTCTCCTTT CTTTGGGAAT 
GAAGCCAACA AGCCACTTCT GCTGCCCAAA ACTCAGGTGA TTGACCGTGC AGTAAAGGTT 
CTGGACCAAA TGCCCCCTTA TGACACCCAT AAAATTGGGG TGGTGTTTGT AGGAGCTGGT 
CAGGTTAACA ATGAAGTCGC CATCTTGTCG AACGAGTACG GTTCGAACCG CTACGCCTCT 
TTCCTCACAG GTCTTGGCAA ATTAATCCAT CTGAAAGACT GCGATCCAGA CCAGATTTTT 
CTTGGAGGGC TTGACCAGTA TGGAGATGAT GGAGAGTTCA CCTACTGCTG GCATGACGAC 
ATCATGCAGG CTATCTTTCA CATAGCCACA CTGATGCCAA ACAGGGAGAG CGACAAGGGT 
TGCTGCAACA AAAAGCGACA CATTGGCAAC GATTTTGTCA TGGTGGTGTA CAATGACTCT 
GGAGAGGAGT ACAAACTGGG CACAATTAAG GGTCAGTTTA ATTTTGTCGA AGTCATTATA 
AAACCACTGG ATTATGAATG TAACCTCGTT ACCCTCCAGT GCCGCAAAGA CCTCGAGGGA 
TTAGTAGACA CAACGGTGGC AAAAATTGTT TCAGATCGAA ACCTTCCCCT GTTAGTCAGG 
CAGATGGCTC TGCATGCAAA TATGGCCTCA TTGGTGCACC AGTACAGAGC TAACCCCTCT 
GACGCATACG CCTCTAAGTG GCTAGCAAGG TTACGACACA TCAAGAGGAT CAGGACCAGG 
GCTCAAGAAG CCATCCAGTC TCGCTCAACT CCCGGGATTT CACTGACCCA AGGGCAGAGC 
AAACCTTTGT CACAGAGCAG TGCTCCAACA AATGTGGAAG CCACCGGACA ACGAAAAAGA 
CTGGTGTCCA CTGTAGACGA TTTCACAGAT TTTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	tsc2
Entrez Gene ID	101468908
Full Name	TSC complex subunit 2
Gene Type	protein-coding
Organism	Maylandia zebra(zebra mbuna)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

tsc2 cDNA ORF clone, Maylandia zebra(zebra mbuna)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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