RAB3GAP1 cDNA ORF clone, Leptonychotes weddellii(Weddell seal)

Gene
Clones
gRNAs

Summary

Gene Symbol	RAB3GAP1
Entrez Gene ID	102728634
Full Name	RAB3 GTPase activating protein catalytic subunit 1
Gene Type	protein-coding
Organism	Leptonychotes weddellii(Weddell seal)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006737751.1	XP_006737814.1	rab3 GTPase-activating protein catalytic subunit
XM_031032928.1	XP_030888788.1	rab3 GTPase-activating protein catalytic subunit

Pathways from BioSystems
Gene Ontology

The following RAB3GAP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAB3GAP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLe106435	XM_006737751.1 Latest version!	Leptonychotes weddellii RAB3 GTPase activating protein subunit 1 (catalytic) (RAB3GAP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OLe195659	XM_031032928.1 Latest version!	Leptonychotes weddellii RAB3 GTPase activating protein catalytic subunit 1 (RAB3GAP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RAB3GAP1 ( XM_006737751.1 ) cDNA ORF clone, Leptonychotes weddellii(Weddell seal) -> XP_006737814.1 Leptonychotes weddellii RAB3 GTPase activating protein subunit 1 (catalytic) (RAB3GAP1), mRNA.

Price & Availability↑

CloneID	OLe106435
Clone ID Related Accession (Same CDS sequence)	XM_006737751.1
Accession Version	XM_006737751.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2952bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-02-13
Organism	Leptonychotes weddellii(Weddell seal)
Product	rab3 GTPase-activating protein catalytic subunit
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006383941.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Leptonychotes weddellii Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCG ACAGTGAGCC CGAGTCCGAG GTATTTGAGA TCACGGACTT CACTACTGCC 
TCGGAATGGG AAAGGTTTAT TTCCAAAGTT GAAGAAGTCT TAAATGATTG GAAACTGATT 
GGAAACTCTT TGGGAAAGCC ACTTGAAAAG AAGGAGGATT ATATTTTACA TGATAGGCCA 
TTTAAGATGA AATTAAATTC TGGTAAAGAA CATGTTATAA AATTTTGCCT TAAAATGAAC 
ATACTAATTT TTTCACTATT AAATGTTTTT GTAGATGTTA TTCCACAATC TATGCAAGAT 
TTGTTGTGTA TGAATAATGA CTTTCCTCCA AGAGCACATT GCCTGGTAAG ATGGTATGGA 
CTGCGGGAGT TTGTGGTGAT TGCCCCTGCT GCAAACCATG ATGCAGTTCT CAGTGAATCT 
AAGTGCAATC TTCTTCTGAG TTCTGTGTCT ATTGCTTTGG GAAACACTGG CTGCCAGGTG 
CCACTCTTTG TGCAAATTCA TCACAAATGG CGAAGAATGT ATGTGGGAGA ATGTCAGGGT 
CATGGTGTCC GAACTGATTT TGAAATGGTT CATCTTCGGA AAGTGCCAAA TCAGTACACT 
CATTTATCAG GTCTGCTGGA TATCTTCAAA TCAAAGATTG GATGTCCTTT AACTCCTTTG 
CCTCCAGTTA GTATTGCTAT TCGCTTTACG TATGTACTTC AGGATTGGCA GCAGTATTTT 
TGGCCTCAGC AACCTCCAGA TATAGATGCC CTTGTAGGAG GGGAAGTTGG AGGCCTGGAG 
TTTGGCAAAT TACCATTTGG TGCCTGTGAA GATCCTATAA GTGAACTCCA TTTAGCAACT 
ACATGGCCTC ACCTGACAGA AGGTATCATT GTGGATAATG ATGTTTATTC CACCGCTTTC 
ATGGCCTTCA AAGCTTTTGC TTCAGCTTCG TCTTTGGGAG GGGCAGGGGC TTCCTTCTTG 
ACCTTTGGTG ATTTTGTCAC TGAATTCTTT AAAATTTGCC GTCGAAAGGA GTCAACTGAT 
GAAATTCTTG GACGATCCAC ATTTGAGGAA GAAGGGAGAG AAATTGCTGA TATAACTCAT 
GCTTTGTCAA AGTTGACAGA GCCAGCACCG GTTCCAATTC ATAAATTATC AGTTTCAAAT 
ATGGTACACA GTGCAAAGAA GAAAATACGA AAACACAGAG GTGTAGAAGA GTCACCACTG 
AATAATGATG TCCTCAATAC TATTCTCTTG TTCTTATTCC CTGATGCTGC TTCTGAGAAA 
CCATTAGATG GAAGTTCTTC CACAGACAAC AATAATCCTG TATCAGAGAG TGAAGATTAT 
AATCTCTACA ATCAGTTCAA ATCTGCACCA TCTGACAGTT TAACATACAA ATTGGCTTTG 
TGTCTTTGTA TGATCAATTT CTATCATGGA GGATTAAAAG GAGTGGCACA TCTCTGGCAG 
GAATTTGTTC TTGAAATGCG TTTCAGATGG GAAAACAACT TTCTGATTCC AGGATTAGCA 
AGTGGACCCC CAGATCTAAG ATGTTGTTTA CTACATCAGA AACTACAGAT GTTAAATTGT 
TGTATTGAAA GAAAGAAGGC TCGTGATGAG GGGAGAAAGA CAAATACTTC AGATATGTAT 
CCAGGGGATG CTGGAAGAGC TGGAGACCAG CTGGGGCCAG ATAATCTAAA AGATATGGAT 
AAGGAAAAGG GAGAGGTAGG AAAATCTTGG GATTCTTGGA GTGACAGTGA AGAAGAATTT 
TTTGAATGCC TAAGTGATAC TGAAGAATTT AAGGGAAACG GACAAGAGAG TAACAAGAAA 
GGAGGACCTA AGGAGATGAC AAGCTTAAAA CCAGAGGGAC GATTATATCA GCATGGAAAA 
CTCACACTGC TACATAATGG AGAACCTCTC TACATTCCAG TGACCCAGGA ACCAGCGCCT 
ATGACAGAAG ATCTGCTAGA AGAGCAGTCG GAGGTTCTAG CTAAATTAGG TACATCAGCA 
GAAGGGGCTC ACCTCCGAGC ACGCATGCAG AGTGCCTGTC TGCTTTCAGA TATGGAATCT 
TTTAAGGCAG CTAATCCAGG TTGTTTTCTG GAAGATTTTG TGAGGTGGTA CTCACCCCGG 
GATTATATTG AAGAGGAGGT CATTGACGAA AAGGGCAACA TGGTTCTGAA AGGAGAACTG 
AGTGCCCGAA TGAAGATTCC AAGCAATATG TGGGTAGAAG CCTGGGAAAC AGCTAAGCCA 
ATTCCTGCTA GGAGGCAAAG GAGACTTTTT GATGATACAC GGGAAGCAGA AAAGGTGCTG 
CACTATCTGG CCGTACAGAA ACCTGCAGAC CTCGCTCGAC ACCTGTTACC TTGTGTAATT 
CACGCAGCTG TACTCAAGGT AAAGGAAGAA GAAAATCTGG AAAACATTTC TTCAGTTAAG 
AAGATTATAA AGCAGATAAT AACCCATTCC AGTAAAGTTT TGCACTTCCC CAATCCAGAA 
GACAAGAAAT TGGAAGAAAT CATCCATCAA ATTACTAATG TAGAAGCTAT AATTGCCAGA 
GCCCGCTCAC TGAAAGCCAA GTTTGGAACT GAGAAATGTG AACAAGAGGA GGAAAAAGAA 
GATCTTGAAA GGTTTGTGAG TTGCCTTTTG GAGCAGCCTG AAGTGTTAGT CGTGGGTGCA 
GGAAGAGGCC ATGCTGGCAG AATCATTCAC AAGCTGTTCG TGAATGCTCA CAGGCTGACT 
GAATCTTCTG ATGAGGCTGC AGCCATGGCT CCACCGGAGG AGGAACTGAA GCGAATGGGC 
TCTCCAGAAG AGAGAAGGCA GAACTCCGTG TCAGACTTCC CACCCCCTGC TGGCCGGGAA 
CTCATTTTGC GCACCACCGT GCCACGCCCG GCTCCGTACT CCCGGGCTCT GCCTCAGCGC 
ATGTACAGTG TTCTCACCAA AGAGGATTTC AGACTTGCAG GTGCCTTTTC ATCAGATACC 
TCCTTCTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006737814.1
CDS	1..2952
Translation	MAADSEPESEVFEITDFTTASEWERFISKVEEVLNDWKLIGNSLGKPLEKKEDYILHDRPFKMKLNSGKEHVIKFCLKMNILIFSLLNVFVDVIPQSMQDLLCMNNDFPPRAHCLVRWYGLREFVVIAPAANHDAVLSESKCNLLLSSVSIALGNTGCQVPLFVQIHHKWRRMYVGECQGHGVRTDFEMVHLRKVPNQYTHLSGLLDIFKSKIGCPLTPLPPVSIAIRFTYVLQDWQQYFWPQQPPDIDALVGGEVGGLEFGKLPFGACEDPISELHLATTWPHLTEGIIVDNDVYSTAFMAFKAFASASSLGGAGASFLTFGDFVTEFFKICRRKESTDEILGRSTFEEEGREIADITHALSKLTEPAPVPIHKLSVSNMVHSAKKKIRKHRGVEESPLNNDVLNTILLFLFPDAASEKPLDGSSSTDNNNPVSESEDYNLYNQFKSAPSDSLTYKLALCLCMINFYHGGLKGVAHLWQEFVLEMRFRWENNFLIPGLASGPPDLRCCLLHQKLQMLNCCIERKKARDEGRKTNTSDMYPGDAGRAGDQLGPDNLKDMDKEKGEVGKSWDSWSDSEEEFFECLSDTEEFKGNGQESNKKGGPKEMTSLKPEGRLYQHGKLTLLHNGEPLYIPVTQEPAPMTEDLLEEQSEVLAKLGTSAEGAHLRARMQSACLLSDMESFKAANPGCFLEDFVRWYSPRDYIEEEVIDEKGNMVLKGELSARMKIPSNMWVEAWETAKPIPARRQRRLFDDTREAEKVLHYLAVQKPADLARHLLPCVIHAAVLKVKEEENLENISSVKKIIKQIITHSSKVLHFPNPEDKKLEEIIHQITNVEAIIARARSLKAKFGTEKCEQEEEKEDLERFVSCLLEQPEVLVVGAGRGHAGRIIHKLFVNAHRLTESSDEAAAMAPPEEELKRMGSPEERRQNSVSDFPPPAGRELILRTTVPRPAPYSRALPQRMYSVLTKEDFRLAGAFSSDTSFF

Target ORF information:

RefSeq Version	XM_006737751.1
Organism	Leptonychotes weddellii(Weddell seal)
Definition	Leptonychotes weddellii RAB3 GTPase activating protein subunit 1 (catalytic) (RAB3GAP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006737751.1

ORF Insert Sequence:

ATGGCGGCCG ACAGTGAGCC CGAGTCCGAG GTATTTGAGA TCACGGACTT CACTACTGCC 
TCGGAATGGG AAAGGTTTAT TTCCAAAGTT GAAGAAGTCT TAAATGATTG GAAACTGATT 
GGAAACTCTT TGGGAAAGCC ACTTGAAAAG AAGGAGGATT ATATTTTACA TGATAGGCCA 
TTTAAGATGA AATTAAATTC TGGTAAAGAA CATGTTATAA AATTTTGCCT TAAAATGAAC 
ATACTAATTT TTTCACTATT AAATGTTTTT GTAGATGTTA TTCCACAATC TATGCAAGAT 
TTGTTGTGTA TGAATAATGA CTTTCCTCCA AGAGCACATT GCCTGGTAAG ATGGTATGGA 
CTGCGGGAGT TTGTGGTGAT TGCCCCTGCT GCAAACCATG ATGCAGTTCT CAGTGAATCT 
AAGTGCAATC TTCTTCTGAG TTCTGTGTCT ATTGCTTTGG GAAACACTGG CTGCCAGGTG 
CCACTCTTTG TGCAAATTCA TCACAAATGG CGAAGAATGT ATGTGGGAGA ATGTCAGGGT 
CATGGTGTCC GAACTGATTT TGAAATGGTT CATCTTCGGA AAGTGCCAAA TCAGTACACT 
CATTTATCAG GTCTGCTGGA TATCTTCAAA TCAAAGATTG GATGTCCTTT AACTCCTTTG 
CCTCCAGTTA GTATTGCTAT TCGCTTTACG TATGTACTTC AGGATTGGCA GCAGTATTTT 
TGGCCTCAGC AACCTCCAGA TATAGATGCC CTTGTAGGAG GGGAAGTTGG AGGCCTGGAG 
TTTGGCAAAT TACCATTTGG TGCCTGTGAA GATCCTATAA GTGAACTCCA TTTAGCAACT 
ACATGGCCTC ACCTGACAGA AGGTATCATT GTGGATAATG ATGTTTATTC CACCGCTTTC 
ATGGCCTTCA AAGCTTTTGC TTCAGCTTCG TCTTTGGGAG GGGCAGGGGC TTCCTTCTTG 
ACCTTTGGTG ATTTTGTCAC TGAATTCTTT AAAATTTGCC GTCGAAAGGA GTCAACTGAT 
GAAATTCTTG GACGATCCAC ATTTGAGGAA GAAGGGAGAG AAATTGCTGA TATAACTCAT 
GCTTTGTCAA AGTTGACAGA GCCAGCACCG GTTCCAATTC ATAAATTATC AGTTTCAAAT 
ATGGTACACA GTGCAAAGAA GAAAATACGA AAACACAGAG GTGTAGAAGA GTCACCACTG 
AATAATGATG TCCTCAATAC TATTCTCTTG TTCTTATTCC CTGATGCTGC TTCTGAGAAA 
CCATTAGATG GAAGTTCTTC CACAGACAAC AATAATCCTG TATCAGAGAG TGAAGATTAT 
AATCTCTACA ATCAGTTCAA ATCTGCACCA TCTGACAGTT TAACATACAA ATTGGCTTTG 
TGTCTTTGTA TGATCAATTT CTATCATGGA GGATTAAAAG GAGTGGCACA TCTCTGGCAG 
GAATTTGTTC TTGAAATGCG TTTCAGATGG GAAAACAACT TTCTGATTCC AGGATTAGCA 
AGTGGACCCC CAGATCTAAG ATGTTGTTTA CTACATCAGA AACTACAGAT GTTAAATTGT 
TGTATTGAAA GAAAGAAGGC TCGTGATGAG GGGAGAAAGA CAAATACTTC AGATATGTAT 
CCAGGGGATG CTGGAAGAGC TGGAGACCAG CTGGGGCCAG ATAATCTAAA AGATATGGAT 
AAGGAAAAGG GAGAGGTAGG AAAATCTTGG GATTCTTGGA GTGACAGTGA AGAAGAATTT 
TTTGAATGCC TAAGTGATAC TGAAGAATTT AAGGGAAACG GACAAGAGAG TAACAAGAAA 
GGAGGACCTA AGGAGATGAC AAGCTTAAAA CCAGAGGGAC GATTATATCA GCATGGAAAA 
CTCACACTGC TACATAATGG AGAACCTCTC TACATTCCAG TGACCCAGGA ACCAGCGCCT 
ATGACAGAAG ATCTGCTAGA AGAGCAGTCG GAGGTTCTAG CTAAATTAGG TACATCAGCA 
GAAGGGGCTC ACCTCCGAGC ACGCATGCAG AGTGCCTGTC TGCTTTCAGA TATGGAATCT 
TTTAAGGCAG CTAATCCAGG TTGTTTTCTG GAAGATTTTG TGAGGTGGTA CTCACCCCGG 
GATTATATTG AAGAGGAGGT CATTGACGAA AAGGGCAACA TGGTTCTGAA AGGAGAACTG 
AGTGCCCGAA TGAAGATTCC AAGCAATATG TGGGTAGAAG CCTGGGAAAC AGCTAAGCCA 
ATTCCTGCTA GGAGGCAAAG GAGACTTTTT GATGATACAC GGGAAGCAGA AAAGGTGCTG 
CACTATCTGG CCGTACAGAA ACCTGCAGAC CTCGCTCGAC ACCTGTTACC TTGTGTAATT 
CACGCAGCTG TACTCAAGGT AAAGGAAGAA GAAAATCTGG AAAACATTTC TTCAGTTAAG 
AAGATTATAA AGCAGATAAT AACCCATTCC AGTAAAGTTT TGCACTTCCC CAATCCAGAA 
GACAAGAAAT TGGAAGAAAT CATCCATCAA ATTACTAATG TAGAAGCTAT AATTGCCAGA 
GCCCGCTCAC TGAAAGCCAA GTTTGGAACT GAGAAATGTG AACAAGAGGA GGAAAAAGAA 
GATCTTGAAA GGTTTGTGAG TTGCCTTTTG GAGCAGCCTG AAGTGTTAGT CGTGGGTGCA 
GGAAGAGGCC ATGCTGGCAG AATCATTCAC AAGCTGTTCG TGAATGCTCA CAGGCTGACT 
GAATCTTCTG ATGAGGCTGC AGCCATGGCT CCACCGGAGG AGGAACTGAA GCGAATGGGC 
TCTCCAGAAG AGAGAAGGCA GAACTCCGTG TCAGACTTCC CACCCCCTGC TGGCCGGGAA 
CTCATTTTGC GCACCACCGT GCCACGCCCG GCTCCGTACT CCCGGGCTCT GCCTCAGCGC 
ATGTACAGTG TTCTCACCAA AGAGGATTTC AGACTTGCAG GTGCCTTTTC ATCAGATACC 
TCCTTCTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAB3GAP1 ( XM_031032928.1 ) cDNA ORF clone, Leptonychotes weddellii(Weddell seal) -> XP_030888788.1 Leptonychotes weddellii RAB3 GTPase activating protein catalytic subunit 1 (RAB3GAP1), mRNA.

Price & Availability↑

CloneID	OLe195659
Clone ID Related Accession (Same CDS sequence)	XM_031032928.1
Accession Version	XM_031032928.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-29
Organism	Leptonychotes weddellii(Weddell seal)
Product	rab3 GTPase-activating protein catalytic subunit
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006383941.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Leptonychotes weddellii Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 4% of CDS bases ##RefSeq-Attributes-END##

ATGGCGGCCG ACAGTGAGCC CGAGTCCGAG GTATTTGAGA TCACGGACTT CACTACTGCC 
TCGGAATGGG AAAGGTTTAT TTCCAAAGTT GAAGAAGTCT TAAATGATTG GAAACTGATT 
GGAAACTCTT TGGGAAAGCC ACTTGAAAAG AAGGAGGATT ATATTTTACA TGATAGGCCA 
TTTAAGATGA AATTAAATTC TGGTAAAGAA CATGTTATAA AATTTTGCCT TAAAATGAAC 
ATACTAATTT TTTCACTATT AAATGTTTTT GTAGATGTTA TTCCACAATC TATGCAAGAT 
TTGTTGTGTA TGAATAATGA CTTTCCTCCA AGAGCACATT GCCTGGTAAG ATGGTATGGA 
CTGCGGGAGT TTGTGGTGAT TGCCCCTGCT GCAAACCATG ATGCAGTTCT CAGTGAATCT 
AAGTGCAATC TTCTTCTGAG TTCTGTGTCT ATTGCTTTGG GAAACACTGG CTGCCAGGTG 
CCACTCTTTG TGCAAATTCA TCACAAATGG CGAAGAATGT ATGTGGGAGA ATGTCAGGGT 
CATGGTGTCC GAACTGATTT TGAAATGGTT CATCTTCGGA AAGTGCCAAA TCAGTACACT 
CATTTATCAG GTCTGCTGGA TATCTTCAAA TCAAAGATTG GATGTCCTTT AACTCCTTTG 
CCTCCAGTTA GTATTGCTAT TCGCTTTACG TATGTACTTC AGGATTGGCA GCAGTATTTT 
TGGCCTCAGC AACCTCCAGA TATAGATGCC CTTGTAGGAG GGGAAGTTGG AGGCCTGGAG 
TTTGGCAAAT TACCATTTGG TGCCTGTGAA GATCCTATAA GTGAACTCCA TTTAGCAACT 
ACATGGCCTC ACCTGACAGA AGGTATCATT GTGGATAATG ATGTTTATTC TTTCTATGTA 
GGTGATTTTG TCACTGAATT CTTTAAAATT TGCCGTCGAA AGGAGTCAAC TGATGAAATT 
CTTGGACGAT CCACATTTGA GGAAGAAGGG AGAGAAATTG CTGATATAAC TCATGCTTTG 
TCAAAGTTGA CAGAGCCAGC ACCGGTTCCA ATTCATAAAT TATCAGTTTC AAATATGGTA 
CACAGTGCAA AGAAGAAAAT ACGAAAACAC AGAGGTGTAG AAGAGTCACC ACTGAATAAT 
GATGTCCTCA ATACTATTCT CTTGTTCTTA TTCCCTGATG CTGCTTCTGA GAAACCATTA 
GATGGAAGTT CTTCCACAGA CAACAATAAT CCTGTATCAG AGAGTGAAGA TTATAATCTC 
TACAATCAGT TCAAATCTGC ACCATCTGAC AGTTTAACAT ACAAATTGGC TTTGTGTCTT 
TGTATGATCA ATTTCTATCA TGGAGGATTA AAAGGAGTGG CACATCTCTG GCAGGAATTT 
GTTCTTGAAA TGCGTTTCAG ATGGGAAAAC AACTTTCTGA TTCCAGGATT AGCAAGTGGA 
CCCCCAGATC TAAGATGTTG TTTACTACAT CAGAAACTAC AGATGTTAAA TTGTTGTATT 
GAAAGAAAGA AGGCTCGTGA TGAGGGGAGA AAGACAAATA CTTCAGATAT GTATCCAGGG 
GATGCTGGAA GAGCTGGAGA CCAGCTGGGG CCAGATAATC TAAAAGATAT GGATAAGGAA 
AAGGGAGAGG TAGGAAAATC TTGGGATTCT TGGAGTGACA GTGAAGAAGA ATTTTTTGAA 
TGCCTAAGTG ATACTGAAGA ATTTAAGGGA AACGGACAAG AGAGTAACAA GAAAGGAGGA 
CCTAAGGAGA TGACAAGCTT AAAACCAGAG GGACGATTAT ATCAGCATGG AAAACTCACA 
CTGCTACATA ATGGAGAACC TCTCTACATT CCAGTGACCC AGGAACCAGC GCCTATGACA 
GAAGATCTGC TAGAAGAGCA GTCGGAGGTT CTAGCTAAAT TAGGTACATC AGCAGAAGGG 
GCTCACCTCC GAGCACGCAT GCAGAGTGCC TGTCTGCTTT CAGATATGGA ATCTTTTAAG 
GCAGCTAATC CAGGTTGTTT TCTGGAAGAT TTTGTGAGGT GGTACTCACC CCGGGATTAT 
ATTGAAGAGG AGGTCATTGA CGAAAAGGGC AACATGGTTC TGAAAGGAGA ACTGAGTGCC 
CGAATGAAGA TTCCAAGCAA TATGTGGGTA GAAGCCTGGG AAACAGCTAA GCCAATTCCT 
GCTAGGAGGC AAAGGAGACT TTTTGATGAT ACACGGGAAG CAGAAAAGGT GCTGCACTAT 
CTGGCCGTAC AGAAACCTGC AGACCTCGCT CGACACCTGT TACCTTGTGT AATTCACGCA 
GCTGTACTCA AGGTAAAGGA AGAAGAAAAT CTGGAAAACA TTTCTTCAGT TAAGAAGATT 
ATAAAGCAGA TAATAACCCA TTCCAGTAAA GTTTTGCACT TCCCCAATCC AGAAGACAAG 
AAATTGGAAG AAATCATCCA TCAAATTACT AATGTAGAAG CTATAATTGC CAGAGCCCGC 
TCACTGAAAG CCAAGTTTGG AACTGAGAAA TGTGAACAAG AGGAGGAAAA AGAAGATCTT 
GAAAGGTTTG TGAGTTGCCT TTTGGAGCAG CCTGAAGTGT TAGTCGTGGG TGCAGGAAGA 
GGCCATGCTG GCAGAATCAT TCACAAGCTG TTCGTGAATG CTCACAGGCT GACTGAATCT 
TCTGATGAGG CTGCAGCCAT GGCTCCACCG GAGGAGGAAC TGAAGCGAAT GGGCTCTCCA 
GAAGAGAGAA GGCAGAACTC CGTGTCAGAC TTCCCACCCC CTGCTGGCCG GGAACTCATT 
TTGCGCACCA CCGTGCCACG CCCGGCTCCG TACTCCCGGG CTCTGCCTCA GCGCATGTAC 
AGTGTTCTCA CCAAAGAGGA TTTCAGACTT GCAGGTGCCT TTTCATCAGA TACCTCCTTC 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030888788.1
CDS	70..2955
Translation	MAADSEPESEVFEITDFTTASEWERFISKVEEVLNDWKLIGNSLGKPLEKKEDYILHDRPFKMKLNSGKEHVIKFCLKMNILIFSLLNVFVDVIPQSMQDLLCMNNDFPPRAHCLVRWYGLREFVVIAPAANHDAVLSESKCNLLLSSVSIALGNTGCQVPLFVQIHHKWRRMYVGECQGHGVRTDFEMVHLRKVPNQYTHLSGLLDIFKSKIGCPLTPLPPVSIAIRFTYVLQDWQQYFWPQQPPDIDALVGGEVGGLEFGKLPFGACEDPISELHLATTWPHLTEGIIVDNDVYSFYVGDFVTEFFKICRRKESTDEILGRSTFEEEGREIADITHALSKLTEPAPVPIHKLSVSNMVHSAKKKIRKHRGVEESPLNNDVLNTILLFLFPDAASEKPLDGSSSTDNNNPVSESEDYNLYNQFKSAPSDSLTYKLALCLCMINFYHGGLKGVAHLWQEFVLEMRFRWENNFLIPGLASGPPDLRCCLLHQKLQMLNCCIERKKARDEGRKTNTSDMYPGDAGRAGDQLGPDNLKDMDKEKGEVGKSWDSWSDSEEEFFECLSDTEEFKGNGQESNKKGGPKEMTSLKPEGRLYQHGKLTLLHNGEPLYIPVTQEPAPMTEDLLEEQSEVLAKLGTSAEGAHLRARMQSACLLSDMESFKAANPGCFLEDFVRWYSPRDYIEEEVIDEKGNMVLKGELSARMKIPSNMWVEAWETAKPIPARRQRRLFDDTREAEKVLHYLAVQKPADLARHLLPCVIHAAVLKVKEEENLENISSVKKIIKQIITHSSKVLHFPNPEDKKLEEIIHQITNVEAIIARARSLKAKFGTEKCEQEEEKEDLERFVSCLLEQPEVLVVGAGRGHAGRIIHKLFVNAHRLTESSDEAAAMAPPEEELKRMGSPEERRQNSVSDFPPPAGRELILRTTVPRPAPYSRALPQRMYSVLTKEDFRLAGAFSSDTSFF

Target ORF information:

RefSeq Version	XM_031032928.1
Organism	Leptonychotes weddellii(Weddell seal)
Definition	Leptonychotes weddellii RAB3 GTPase activating protein catalytic subunit 1 (RAB3GAP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031032928.1

ORF Insert Sequence:

ATGGCGGCCG ACAGTGAGCC CGAGTCCGAG GTATTTGAGA TCACGGACTT CACTACTGCC 
TCGGAATGGG AAAGGTTTAT TTCCAAAGTT GAAGAAGTCT TAAATGATTG GAAACTGATT 
GGAAACTCTT TGGGAAAGCC ACTTGAAAAG AAGGAGGATT ATATTTTACA TGATAGGCCA 
TTTAAGATGA AATTAAATTC TGGTAAAGAA CATGTTATAA AATTTTGCCT TAAAATGAAC 
ATACTAATTT TTTCACTATT AAATGTTTTT GTAGATGTTA TTCCACAATC TATGCAAGAT 
TTGTTGTGTA TGAATAATGA CTTTCCTCCA AGAGCACATT GCCTGGTAAG ATGGTATGGA 
CTGCGGGAGT TTGTGGTGAT TGCCCCTGCT GCAAACCATG ATGCAGTTCT CAGTGAATCT 
AAGTGCAATC TTCTTCTGAG TTCTGTGTCT ATTGCTTTGG GAAACACTGG CTGCCAGGTG 
CCACTCTTTG TGCAAATTCA TCACAAATGG CGAAGAATGT ATGTGGGAGA ATGTCAGGGT 
CATGGTGTCC GAACTGATTT TGAAATGGTT CATCTTCGGA AAGTGCCAAA TCAGTACACT 
CATTTATCAG GTCTGCTGGA TATCTTCAAA TCAAAGATTG GATGTCCTTT AACTCCTTTG 
CCTCCAGTTA GTATTGCTAT TCGCTTTACG TATGTACTTC AGGATTGGCA GCAGTATTTT 
TGGCCTCAGC AACCTCCAGA TATAGATGCC CTTGTAGGAG GGGAAGTTGG AGGCCTGGAG 
TTTGGCAAAT TACCATTTGG TGCCTGTGAA GATCCTATAA GTGAACTCCA TTTAGCAACT 
ACATGGCCTC ACCTGACAGA AGGTATCATT GTGGATAATG ATGTTTATTC TTTCTATGTA 
GGTGATTTTG TCACTGAATT CTTTAAAATT TGCCGTCGAA AGGAGTCAAC TGATGAAATT 
CTTGGACGAT CCACATTTGA GGAAGAAGGG AGAGAAATTG CTGATATAAC TCATGCTTTG 
TCAAAGTTGA CAGAGCCAGC ACCGGTTCCA ATTCATAAAT TATCAGTTTC AAATATGGTA 
CACAGTGCAA AGAAGAAAAT ACGAAAACAC AGAGGTGTAG AAGAGTCACC ACTGAATAAT 
GATGTCCTCA ATACTATTCT CTTGTTCTTA TTCCCTGATG CTGCTTCTGA GAAACCATTA 
GATGGAAGTT CTTCCACAGA CAACAATAAT CCTGTATCAG AGAGTGAAGA TTATAATCTC 
TACAATCAGT TCAAATCTGC ACCATCTGAC AGTTTAACAT ACAAATTGGC TTTGTGTCTT 
TGTATGATCA ATTTCTATCA TGGAGGATTA AAAGGAGTGG CACATCTCTG GCAGGAATTT 
GTTCTTGAAA TGCGTTTCAG ATGGGAAAAC AACTTTCTGA TTCCAGGATT AGCAAGTGGA 
CCCCCAGATC TAAGATGTTG TTTACTACAT CAGAAACTAC AGATGTTAAA TTGTTGTATT 
GAAAGAAAGA AGGCTCGTGA TGAGGGGAGA AAGACAAATA CTTCAGATAT GTATCCAGGG 
GATGCTGGAA GAGCTGGAGA CCAGCTGGGG CCAGATAATC TAAAAGATAT GGATAAGGAA 
AAGGGAGAGG TAGGAAAATC TTGGGATTCT TGGAGTGACA GTGAAGAAGA ATTTTTTGAA 
TGCCTAAGTG ATACTGAAGA ATTTAAGGGA AACGGACAAG AGAGTAACAA GAAAGGAGGA 
CCTAAGGAGA TGACAAGCTT AAAACCAGAG GGACGATTAT ATCAGCATGG AAAACTCACA 
CTGCTACATA ATGGAGAACC TCTCTACATT CCAGTGACCC AGGAACCAGC GCCTATGACA 
GAAGATCTGC TAGAAGAGCA GTCGGAGGTT CTAGCTAAAT TAGGTACATC AGCAGAAGGG 
GCTCACCTCC GAGCACGCAT GCAGAGTGCC TGTCTGCTTT CAGATATGGA ATCTTTTAAG 
GCAGCTAATC CAGGTTGTTT TCTGGAAGAT TTTGTGAGGT GGTACTCACC CCGGGATTAT 
ATTGAAGAGG AGGTCATTGA CGAAAAGGGC AACATGGTTC TGAAAGGAGA ACTGAGTGCC 
CGAATGAAGA TTCCAAGCAA TATGTGGGTA GAAGCCTGGG AAACAGCTAA GCCAATTCCT 
GCTAGGAGGC AAAGGAGACT TTTTGATGAT ACACGGGAAG CAGAAAAGGT GCTGCACTAT 
CTGGCCGTAC AGAAACCTGC AGACCTCGCT CGACACCTGT TACCTTGTGT AATTCACGCA 
GCTGTACTCA AGGTAAAGGA AGAAGAAAAT CTGGAAAACA TTTCTTCAGT TAAGAAGATT 
ATAAAGCAGA TAATAACCCA TTCCAGTAAA GTTTTGCACT TCCCCAATCC AGAAGACAAG 
AAATTGGAAG AAATCATCCA TCAAATTACT AATGTAGAAG CTATAATTGC CAGAGCCCGC 
TCACTGAAAG CCAAGTTTGG AACTGAGAAA TGTGAACAAG AGGAGGAAAA AGAAGATCTT 
GAAAGGTTTG TGAGTTGCCT TTTGGAGCAG CCTGAAGTGT TAGTCGTGGG TGCAGGAAGA 
GGCCATGCTG GCAGAATCAT TCACAAGCTG TTCGTGAATG CTCACAGGCT GACTGAATCT 
TCTGATGAGG CTGCAGCCAT GGCTCCACCG GAGGAGGAAC TGAAGCGAAT GGGCTCTCCA 
GAAGAGAGAA GGCAGAACTC CGTGTCAGAC TTCCCACCCC CTGCTGGCCG GGAACTCATT 
TTGCGCACCA CCGTGCCACG CCCGGCTCCG TACTCCCGGG CTCTGCCTCA GCGCATGTAC 
AGTGTTCTCA CCAAAGAGGA TTTCAGACTT GCAGGTGCCT TTTCATCAGA TACCTCCTTC 
TTCTAA