NPL cDNA ORF clone, Latimeria chalumnae(coelacanth)

Gene
Clones
gRNAs

Summary

Gene Symbol	NPL
Entrez Gene ID	102367679
Full Name	N-acetylneuraminate pyruvate lyase
Gene Type	protein-coding
Organism	Latimeria chalumnae(coelacanth)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005997426.2	XP_005997488.1	N-acetylneuraminate lyase

Pathways from BioSystems
Gene Ontology

The following NPL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NPL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLa28333	XM_005997426.2 Latest version!	Latimeria chalumnae N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase) (NPL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NPL ( XM_005997426.2 ) cDNA ORF clone, Latimeria chalumnae(coelacanth) -> XP_005997488.1 Latimeria chalumnae N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase) (NPL), mRNA.

Price & Availability↑

CloneID	OLa28333
Clone ID Related Accession (Same CDS sequence)	XM_005997426.2
Accession Version	XM_005997426.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-10-25
Organism	Latimeria chalumnae(coelacanth)
Product	N-acetylneuraminate lyase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005819395.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Oct 26, 2015 this sequence version replaced XM_005997426.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Latimeria chalumnae Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGAATC CAAAGAACAG AATTAAAGGC CTTGTTGCTG CCACATTCAC ACCTATGGCA 
GAAAATGGGG AAGTCAACTA CTCTATCATT GGCCGGTATG TGGACTACCT TGTACATACA 
CAGGGAGTCA AAAATGTTTT TGTTAATGGT ACTACAGGAG AATGGGCATC TTTGACCGTC 
CAGGAGCGGA AGCTTCTTGC AGAGAAGTGG GTCACAGAAG CAAAAAACAA GTTGGATAAC 
GTGATTGTTC ATGTTGGATC TTTAAATTTA GAGGATGCAA AAGATCTGGC CAGGCATGCT 
GCTGAACTTC AAGTAGATGG GATTTCTGTA GTTTCCCCTT CACTTTTCAA ACCTGCAAGC 
ATAGAGGCAC TGGTGAAATT CCTTCAGGAG GTGGCATCTG CTGCTCCGAA TGTACCTTTC 
TATTATTATC ATATTCCTGA GTTAACTGGA GTGAATATTC AGGCTATAGA TCTTCTAGAC 
AGTTTGAGAG AGAAGATCCC ATCACTCCAG GGGCTGAAGT TCACCGGCAC TGACCTGTTG 
GACTTTGGAC AGTGTGTGCA AAACAGTAAC AATCAGCTTG CTCTGCTTTA TGGGAAGGAT 
GAGCAACTGT TAGCTGCTTT AATCATGGGA GCAACTGGTG CTATTGGCAG CACGTACAAC 
TACCAGGGAA AGTTGATGAA TAATATGCTG GCTGCTTTTG AAAAGAAAGA TTATGACTTA 
GCAAGACAGT ACCAGTTTCA AGCCCAGGAA TTTCTACACT TTGCCATTAA ATTAGGGTTT 
GGAGTGGCAG AAAATAAATG TGTTATGTCT CTTGTATCGG GCATTCCACT GGGGCCTCCT 
CGACTCCCAC TGACAATGTG CACAGAGAAG CTTGCTACAC AGTTCAAGAT GAAGCTGACA 
GTATTGCACC TGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005997488.1
CDS	16..933
Translation	MTNPKNRIKGLVAATFTPMAENGEVNYSIIGRYVDYLVHTQGVKNVFVNGTTGEWASLTVQERKLLAEKWVTEAKNKLDNVIVHVGSLNLEDAKDLARHAAELQVDGISVVSPSLFKPASIEALVKFLQEVASAAPNVPFYYYHIPELTGVNIQAIDLLDSLREKIPSLQGLKFTGTDLLDFGQCVQNSNNQLALLYGKDEQLLAALIMGATGAIGSTYNYQGKLMNNMLAAFEKKDYDLARQYQFQAQEFLHFAIKLGFGVAENKCVMSLVSGIPLGPPRLPLTMCTEKLATQFKMKLTVLHLI

Target ORF information:

RefSeq Version	XM_005997426.2
Organism	Latimeria chalumnae(coelacanth)
Definition	Latimeria chalumnae N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase) (NPL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005997426.2

ORF Insert Sequence:

ATGACGAATC CAAAGAACAG AATTAAAGGC CTTGTTGCTG CCACATTCAC ACCTATGGCA 
GAAAATGGGG AAGTCAACTA CTCTATCATT GGCCGGTATG TGGACTACCT TGTACATACA 
CAGGGAGTCA AAAATGTTTT TGTTAATGGT ACTACAGGAG AATGGGCATC TTTGACCGTC 
CAGGAGCGGA AGCTTCTTGC AGAGAAGTGG GTCACAGAAG CAAAAAACAA GTTGGATAAC 
GTGATTGTTC ATGTTGGATC TTTAAATTTA GAGGATGCAA AAGATCTGGC CAGGCATGCT 
GCTGAACTTC AAGTAGATGG GATTTCTGTA GTTTCCCCTT CACTTTTCAA ACCTGCAAGC 
ATAGAGGCAC TGGTGAAATT CCTTCAGGAG GTGGCATCTG CTGCTCCGAA TGTACCTTTC 
TATTATTATC ATATTCCTGA GTTAACTGGA GTGAATATTC AGGCTATAGA TCTTCTAGAC 
AGTTTGAGAG AGAAGATCCC ATCACTCCAG GGGCTGAAGT TCACCGGCAC TGACCTGTTG 
GACTTTGGAC AGTGTGTGCA AAACAGTAAC AATCAGCTTG CTCTGCTTTA TGGGAAGGAT 
GAGCAACTGT TAGCTGCTTT AATCATGGGA GCAACTGGTG CTATTGGCAG CACGTACAAC 
TACCAGGGAA AGTTGATGAA TAATATGCTG GCTGCTTTTG AAAAGAAAGA TTATGACTTA 
GCAAGACAGT ACCAGTTTCA AGCCCAGGAA TTTCTACACT TTGCCATTAA ATTAGGGTTT 
GGAGTGGCAG AAAATAAATG TGTTATGTCT CTTGTATCGG GCATTCCACT GGGGCCTCCT 
CGACTCCCAC TGACAATGTG CACAGAGAAG CTTGCTACAC AGTTCAAGAT GAAGCTGACA 
GTATTGCACC TGATTTAA