afdn gene cDNA ORF clone, Larimichthys crocea(large yellow croaker)

mRNA	Protein	Name
XM_027284630.1	XP_027140431.1	afadin isoform X10
XM_027284631.1	XP_027140432.1	afadin isoform X11
XM_027284633.1	XP_027140434.1	afadin isoform X13
XM_027284634.1	XP_027140435.1	afadin isoform X14
XM_027284636.1	XP_027140437.1	afadin isoform X15
XM_027284638.1	XP_027140439.1	afadin isoform X17
XM_027284621.1	XP_027140422.1	afadin isoform X1
XM_027284624.1	XP_027140425.1	afadin isoform X4
XM_027284625.1	XP_027140426.1	afadin isoform X5
XM_027284637.1	XP_027140438.1	afadin isoform X16
XM_019265734.1	XP_019121279.1	afadin
XM_027284628.1	XP_027140429.1	afadin isoform X8
XM_027284623.1	XP_027140424.1	afadin isoform X3
XM_027284626.1	XP_027140427.1	afadin isoform X6
XM_027284629.1	XP_027140430.1	afadin isoform X9
XM_027284622.1	XP_027140423.1	afadin isoform X2
XM_027284627.1	XP_027140428.1	afadin isoform X7
XM_027284632.1	XP_027140433.1	afadin isoform X12
XM_027284639.1	XP_027140440.1	afadin isoform X18

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OLa200703	XM_027284621.1 Latest version!	Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa200704	XM_027284622.1 Latest version!	Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa200705	XM_027284623.1 Latest version!	Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa200706	XM_027284624.1 Latest version!	Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa200707	XM_027284625.1 Latest version!	Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa200708	XM_027284626.1 Latest version!	Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa200709	XM_027284627.1 Latest version!	Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa200710	XM_027284628.1 Latest version!	Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa200711	XM_027284629.1 Latest version!	Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa200712	XM_027284630.1 Latest version!	Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa200713	XM_027284631.1 Latest version!	Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa200714	XM_027284632.1 Latest version!	Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa200715	XM_027284633.1 Latest version!	Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa200716	XM_027284634.1 Latest version!	Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa200717	XM_027284636.1 Latest version!	Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa200718	XM_027284637.1 Latest version!	Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa200719	XM_027284638.1 Latest version!	Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa200720	XM_027284639.1 Latest version!	Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa97801	XM_019265734.1 Latest version!	Larimichthys crocea afadin, adherens junction formation factor (afdn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

afdn ( XM_027284621.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_027140422.1 Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OLa200703
Clone ID Related Accession (Same CDS sequence)	XM_027284621.1
Accession Version	XM_027284621.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5895bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	afadin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040021.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGCA GCAGGGAGGA GGAGAGGAGG AAACTGGCCG ACATCATCAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CGGCCCACAG AGGACCTGGA GTTCCATGGC 
GTGATGCGGT TCTACTTCCA GGACCGGGTC GCCGGTAACT TTGCCACCAA ATGCATCAGA 
GTGTCGAGCA CGGCGACAAC GCAGGACGTC ATCGAGACGT TAGCAGAGAA GTTCAGACCG 
GACATGAGGA TGCTCTCCTC GCCAAAATAC TCTTTGTACG AGGTCCACGT CAGTGGCGAA 
GAGCGTCAGC TGGATCTGGA CGAGAAGCCT CTGGTGGTTC AGCTGAACTG GAACAAAGAC 
GACAGAGAAG GACGCTTCGT CCTGAAGAAC GAGAACGACA TCCTCCCCAA GAAGAGTCAG 
AGTAACGGCC CGGAGAAGGA GAAGGACGGA GTGATCCAGA ACTTCAAGAG GACTTTGTCC 
AAGAAGGAGA AGAAGAAGGA GAAGAAGAGG GAGAAGGAGT TCGCCCGGAT CCCCGACGGA 
GACGAGCAGG CGCTCAGCCG AGAGGACGGG GAGAACAGTC GTCTGGCGGC GGAGGTTTAC 
AAGGACATGC CGGAGAACAG CTTCACCAGG ACGATCTCCA ACCCGGAGGT GGTGATGAAG 
AGGAGACGCC AGCAGAAGCT GGAGAAACGC ATGCAGGAGT TCATGAGCAG CGACGGGAGG 
CCCGACTCAG GGGGCACGTT GCGGATATAC GCCGACAGCC TGAAGCCGAA CATCCCGTAT 
AAAACCATCC TGCTGTCCAC GAGAGACACG GCTGACTTTG CGGTGGTGGA GGCGCTGGAG 
AAGTACGGGC TGGAGAAGGA GAACCCCCGA GAGTACTGCA TCGCCCGGCA AGACGATAAG 
TCCGGCAAGG AGGCGATCCT GGACGATACC GAGTGTCCAC TGCAGATCTT CAGGGACTGG 
CCTGCAGACA GAGGAGCGCT AGTGTTCCAG CTGAAGAAGA GACCCCCGGA CTACCAGGGC 
AGGAAGGGGA GGAAAGTGGA CGACAAGGGC CTGCGAGGGA AGGACGGCAG CAGCTCCCTG 
CCGCCCGAGA AACTGCCTTA CCTGGTGGAG CTGAGCCCAG GGCGAGGGAA TCACTATGCC 
TACTACGCCT ATCGGCACCA CGAAGACGGG TCTGACTCAC GGGACAAACC AAAGCTGTAC 
CGGCTCCAGC ACAGCATCAC TGAGGTCGGC TCAGACTGCA CAGAGGACGG AGCCATTCAG 
CTGCTCGGTC CGGGGATTCT GCCCCACCAC TGCAACCTCA TGCACAGTGA AGGCATGGTG 
ACGGTGACGC CGCACGGCCC CGACGCCGAC ACCTTCGTCG ACGGGCAACG GGTCACTGAG 
ACGACGGTGC TGCGCTCGGG CTCCACCCTC CAGTTTGGCC AAGCGCACGT CTTCAAGTTT 
GTGGACCCGA TGTTCGACCA GGGAGGGAAG AGAGAGCCGG CCGCCATGAT GAGGAGTCGA 
CACAAGTCCG GCAGCGTACC CGAAACCACC TTCGACCTTC ACGGAGACGT CCACAGCGGA 
GCCGCGCTGC CTACCAGCAA GAGCTCAGGA AAGCTGGAGA TGGAGAGAGG AATGGTCAAA 
CCGATGATCA GAGGAGAGCA GCAGGACGGC AGGTCTCAGG ACATTTCAGC AGACAGAGCC 
GAGTTGACTC TGCCAGCCGG CATCGAGTTC AGAGACAACT CTGAAGACAC CTTCCTGTCG 
GCCATCATCA ACTACACCAA CAGCTCCACG GTTCACTTCA AACTCTCGCC CACGTACGTC 
CTGTACATGG CCTGCCGCTA CATCCTGTCG CCCTCGTACC GGCCCGACAT GTCGCCGTCC 
GAGAGGACGC ACAAGGTCAT CGCCATCGTC AACAAGATGG TGAGCATGAT GGAGGGAGTC 
ATCCAGGAGA TTCCTGAAGG GGATCAGAAG CAGAAGAACA TCGCGGGAGC GCTGGCTTTC 
TGGATGGCCA ACGCCTCCGA GCTGCTCAAC TTCATCAAGC AGGACCGAGA CCTGAACCGC 
ATCACGCTGG ACGCCCAGGA CATCCTCGCC CATCTGGTTC AGATGGCTTT CAAGTACCTG 
GTCCACTGTC TGCAGGCTGA TCTGAATAAC TACATGCCGG CCTTCCTGGA CGACCCCGAG 
GAACACAACC CACAGAGACC CAAGATCGAG GACGTCCTGC ACACCCTCAC GGGGGCGATG 
TCGTTACTGC GGCGGTGTCG GGTCAACGCC GCTCTCACCA TCCAGCTCTT CTCGCAGCTC 
TTCCACTTCA TCAACATGTG GCTCTTCAAC AAGCTGGTGA CGGACACCGA GTCCGGGCTG 
TGCTGTCACT ACTGGGGGGC CATCCTGCGG CAGCAGCTCA GCCACATCGA GGCCTGGGCG 
GAGAAACAGG GGCTGGAGCT CGCCGCCGAC TGCCACCTCA GTCGAATCGT GCAGGCCACC 
ACCCTGCTGA CCATGGACAA ATACTCCATG CAGGATGTGC CGAACATCCA CAACACCTGC 
TTCAAGCTCA ACTCGCTGCA GCTCCACGCC CTCATGAACA ACTACCACTG CGCTCCGGAC 
GAGCCGTACA TCCCGCCGGA GCTGATAGAC CACGTGGTGG CAGTGGCAGA GAACACAGCA 
GATGAGCTGG CGAGGAGCGA CGGCAGAGAG GTTCAGCTGG AGGAGGATCC GGACCTCCAG 
CTGCCGTTCC TCCTTCCCGA GGACGGTTAC TCCTGCGACG TGGTGCGCAG CCTCCCCAAC 
GGCCTGCAGG ACTTCCTGGA GCCGCTGCTG CAGAGAGGTT TCTGTCGGTT GGTACCTCAC 
CCTCGCTCTC CCGGTACCTG GACGGTCCAT TTTGAAGGAG CTGACTGCGA CAGCCACTTC 
TCTGCTGCTG ACAACTCAGA GATGCCAATG AGGAAAGAGC CGGAGGTTGT CACGGTAACC 
CTGAAGAAGC ACAACGGCAT GGGCCTCAGC ATCGTGGCTG CCAAGGGTGC TGGTCAGGAG 
AAGCTGGGCA TCTACATCAA GTCTGTCGTC AAAGGAGGAG CAGCTGATGT GGACGGGCGT 
CTGGCTGCAG GTGACCAGCT GTTGAGCGTG GACGGGCGGA GTCTGGTCGG CCTATCACAA 
GAGAGGGCAG CGGAGCTGAT GACGAGGACG GGCTCAGTCG TGACTCTGGA GGTCGCCAAG 
CAGGGAGCCA TTTACCACGG ACTGGCCACG CTCCTCAACC AGCCGAGCCC CATGATGCCA 
CGAGCGTCAG ACAGAGGCCG CGACAAGAAC GGGAAACTCC GACCAAAGAG CGAAGGCTTC 
GAGTTGTACA ACAGCTCGGT GCAGAACGGC TCTCCGGAGA GCCCGCAGGC CGGCTGGGAC 
TCTTACCCCG AACCAAAGAA GATGAGCGGC GAAGATCGAC TCCTGAAGAA CCGAGCCGAC 
CACCGCTCCA GCCCCAACGT CGCCAATCAG GGCCAGAGTC CTGCAGGCAA AGCAGTGTAC 
CCCGGAGGAC CTGGCACCAA GATCACCTCC GTCTCCACCG GCAACCTGTG CGCAGACGAT 
GAGCCCTCCC CTCCACGTCC AGAAGCTTAC CCCATCCCCA CCCAGACCTA CCCCAGGGAG 
TACTTCACCA TCCCGGCTTC CAAGAGCCAG GATCGGGTCG TTGGTCCTGG GCAGGGACAG 
CACTGGCAGG GCATGGAGGA CAGAGAACGC CTCCCTGTGG TCGACAACAT CCACAACAGC 
ATGCAGCGGG TTAACCACTC CCAGGAGGAG TTGTACCCTC CACCCGGAGG CCTGAGGCCG 
GAGGAGCGCA TGCAGCAGCA CTACCAGCAG GACTACCAGC ACCGCGACAT GGACTACCAG 
CACAGAGAGC TCGACTACCA GAGAGGAGGT CCACCAGGTG TCGGAGGTCC CGATCACTGG 
ACTCCACAGC CTCAGGTGTC CTCCTCGCTG GAGTCGTCCA CGTCCAGCCA GGAGCACCTC 
AACTTCTCCG CCTCCTCCTC GTCCTCCAAC AAAACCCAGA ACCAGAAGAC CGGGCCGGGC 
CGGTGGAAGA CTCCCAACGC CCCTCACTCC GTGCCGCCAC ATTCAGCCCA GCCGCCCTCC 
CGCTCCGACC TGCCCCCTCC TCCGCCTCCA CCGCCAGCTA CTTACCCCGA ATCCTACGAC 
CCATACGACC CCCAACCGGA CCTGCCGCTC CCGCCGCCTC CCTCCGCCGT CACTCCTGCG 
GTAGCTCAGC AGGCGGCCGA CCGCAAGAAA CGTGAGGAGC AGCAGCGCTG GTATGAGAAG 
GAGAAAGCTC GGCTGGAGGA GGAGAGGGAG AGGAAGAGGA GAGAGCAGGA GAGGAAGCTG 
GGTCAGATCC GGGCCAACCC CGTCATGCCC GTCCAGCCTC ACAACAACGG AGGATCCATG 
CCTCCGCCTC ACCACCAGCC GCCTCTGCCG TCTGCAGCCC CGCCTCAGCC CTACCTTCCC 
CCCGCTCAGC CCCAGCCTCC ACCATCCAGA CCGGAGAAGC TGACCAGCCT GCCGCGGTCA 
GCCGTTCCTC CACCCGCCAG CGCCTCCAAC CCTGCAGGCA TGGACACGGT GATCAGGGAG 
CTGTTGCCAC AGCAGCAGCC GCGGACCATC GAGAGGCGAG ACCTGCAGTA CATCACCATC 
AGCAAGGAGG AGCTCACCTC CAGCGACAGT CTGTCTCCAG ACCCCTGGAA GCGAGACGCC 
CGCGAGAAGG CCGAGAAGCA GCAGCAGCTC CACATCGTCG ACCTGCTGGA CAAGGAGATC 
CAGGAGCTCC AGGCGAAGCC GGAGCGGACG GCGGAGGAGA GCGACCGCCT CAGGAAGCTG 
ATGCTGGAGT GGCAGTTCCA GAAACGACTG CAGGAGTCCA AGCAGAGCGA CGAAGACGAG 
GAGGAGGAGG ACGACGAGGA CGTGGACACC ATGATGATCA TGCAGAGGCT GGAGGCCGAG 
AAGAGAGCCA GGCAGCAGAA TGCTGTCCCA GCTATCTCTG TTCTAGACTT GTTGCAGGAC 
GAGGAGCGGC GGCGTAAGCA GCAGCTGGAG GAGATTCGTA AGCGGGAGGC GGAGGAGCGA 
GCAAAGCAGG AGGAGGAGAG GAGGTGGAGG GAGGAGGAGA GAGCCAAGCG AGAGGCCGAG 
GAGAAGAGGA GACAGGAGGA GGAGTACTAC ACTCGCCTGG AGGCGGAGCG GCGCCGGCAG 
CACGACGAGG CCGAGAGGAA GCTGCTGACG CCCGACGACC CGGCCGGTCT GTATCGGCCC 
CCGCTGCCCC GGGATTACCA AACCCCGCCC CCCCAACAAA CCTATAAAAC CCCCCCGCCC 
CCCCCACAGA GAAACACCTC CTACCTCAAA ACGCAGGTCA CCTCCCCCGA CACGCTCTAC 
ACCGCCAAGT TTGTCTCCTA CGCGGGCGAC GAGGAGGAAG ACGAGGAGGC AGGGAGTGGC 
GGCGGCGGCG GCAGCAGCAG CCAATCAGGT CAGGTGAAGC TGTCGGCGAC CCGGAAGTCG 
TACGGCGACC TCCCGGCCTC CGCCTCGCCC AATCACAGCA GGCCTCAGCC GCCGCCCACT 
GCGCGCAAGC CCCGCCCTGT TTCTGACGGC ATCTTCCTGT CTGGCTCGTT CCAGCCGCCA 
TTCAACAACA ACTCCAACAG TACAGGTCCC CCCCTTCCTG CCAAACCCAG CTCCATCCCC 
CCGGGAGGCT TTAAAGGATT TAACTCGTAC ACTGGAAACT CAGCAGGAGT TGTAGGATCA 
GGAGAAGTCT ACAAGGACCC GCGAGACAAA TGGACGAAGA GTCAGGAGCA GGAGAACTCG 
AACCCCAGCG GCCCTCCGGA GAATCTGACC TTCAAGGAGC GGCAGCGTCT CTTCTCTCAG 
GGGAAGGAGG TTTCCAACAA GGTCAAAGCT TCGCGCAAAC TCATGGAGCT GGAGAACGAG 
CTCAACACCA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027140422.1
CDS	233..6127
Translation	MSGSREEERRKLADIINHWNANRLDLFEISRPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERQLDLDEKPLVVQLNWNKDDREGRFVLKNENDILPKKSQSNGPEKEKDGVIQNFKRTLSKKEKKKEKKREKEFARIPDGDEQALSREDGENSRLAAEVYKDMPENSFTRTISNPEVVMKRRRQQKLEKRMQEFMSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTRDTADFAVVEALEKYGLEKENPREYCIARQDDKSGKEAILDDTECPLQIFRDWPADRGALVFQLKKRPPDYQGRKGRKVDDKGLRGKDGSSSLPPEKLPYLVELSPGRGNHYAYYAYRHHEDGSDSRDKPKLYRLQHSITEVGSDCTEDGAIQLLGPGILPHHCNLMHSEGMVTVTPHGPDADTFVDGQRVTETTVLRSGSTLQFGQAHVFKFVDPMFDQGGKREPAAMMRSRHKSGSVPETTFDLHGDVHSGAALPTSKSSGKLEMERGMVKPMIRGEQQDGRSQDISADRAELTLPAGIEFRDNSEDTFLSAIINYTNSSTVHFKLSPTYVLYMACRYILSPSYRPDMSPSERTHKVIAIVNKMVSMMEGVIQEIPEGDQKQKNIAGALAFWMANASELLNFIKQDRDLNRITLDAQDILAHLVQMAFKYLVHCLQADLNNYMPAFLDDPEEHNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVTDTESGLCCHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVPNIHNTCFKLNSLQLHALMNNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRSLPNGLQDFLEPLLQRGFCRLVPHPRSPGTWTVHFEGADCDSHFSAADNSEMPMRKEPEVVTVTLKKHNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRDKNGKLRPKSEGFELYNSSVQNGSPESPQAGWDSYPEPKKMSGEDRLLKNRADHRSSPNVANQGQSPAGKAVYPGGPGTKITSVSTGNLCADDEPSPPRPEAYPIPTQTYPREYFTIPASKSQDRVVGPGQGQHWQGMEDRERLPVVDNIHNSMQRVNHSQEELYPPPGGLRPEERMQQHYQQDYQHRDMDYQHRELDYQRGGPPGVGGPDHWTPQPQVSSSLESSTSSQEHLNFSASSSSSNKTQNQKTGPGRWKTPNAPHSVPPHSAQPPSRSDLPPPPPPPPATYPESYDPYDPQPDLPLPPPPSAVTPAVAQQAADRKKREEQQRWYEKEKARLEEERERKRREQERKLGQIRANPVMPVQPHNNGGSMPPPHHQPPLPSAAPPQPYLPPAQPQPPPSRPEKLTSLPRSAVPPPASASNPAGMDTVIRELLPQQQPRTIERRDLQYITISKEELTSSDSLSPDPWKRDAREKAEKQQQLHIVDLLDKEIQELQAKPERTAEESDRLRKLMLEWQFQKRLQESKQSDEDEEEEDDEDVDTMMIMQRLEAEKRARQQNAVPAISVLDLLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERAKREAEEKRRQEEEYYTRLEAERRRQHDEAERKLLTPDDPAGLYRPPLPRDYQTPPPQQTYKTPPPPPQRNTSYLKTQVTSPDTLYTAKFVSYAGDEEEDEEAGSGGGGGSSSQSGQVKLSATRKSYGDLPASASPNHSRPQPPPTARKPRPVSDGIFLSGSFQPPFNNNSNSTGPPLPAKPSSIPPGGFKGFNSYTGNSAGVVGSGEVYKDPRDKWTKSQEQENSNPSGPPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNTK

Target ORF information:

RefSeq Version	XM_027284621.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027284621.1

ORF Insert Sequence:

ATGTCGGGCA GCAGGGAGGA GGAGAGGAGG AAACTGGCCG ACATCATCAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CGGCCCACAG AGGACCTGGA GTTCCATGGC 
GTGATGCGGT TCTACTTCCA GGACCGGGTC GCCGGTAACT TTGCCACCAA ATGCATCAGA 
GTGTCGAGCA CGGCGACAAC GCAGGACGTC ATCGAGACGT TAGCAGAGAA GTTCAGACCG 
GACATGAGGA TGCTCTCCTC GCCAAAATAC TCTTTGTACG AGGTCCACGT CAGTGGCGAA 
GAGCGTCAGC TGGATCTGGA CGAGAAGCCT CTGGTGGTTC AGCTGAACTG GAACAAAGAC 
GACAGAGAAG GACGCTTCGT CCTGAAGAAC GAGAACGACA TCCTCCCCAA GAAGAGTCAG 
AGTAACGGCC CGGAGAAGGA GAAGGACGGA GTGATCCAGA ACTTCAAGAG GACTTTGTCC 
AAGAAGGAGA AGAAGAAGGA GAAGAAGAGG GAGAAGGAGT TCGCCCGGAT CCCCGACGGA 
GACGAGCAGG CGCTCAGCCG AGAGGACGGG GAGAACAGTC GTCTGGCGGC GGAGGTTTAC 
AAGGACATGC CGGAGAACAG CTTCACCAGG ACGATCTCCA ACCCGGAGGT GGTGATGAAG 
AGGAGACGCC AGCAGAAGCT GGAGAAACGC ATGCAGGAGT TCATGAGCAG CGACGGGAGG 
CCCGACTCAG GGGGCACGTT GCGGATATAC GCCGACAGCC TGAAGCCGAA CATCCCGTAT 
AAAACCATCC TGCTGTCCAC GAGAGACACG GCTGACTTTG CGGTGGTGGA GGCGCTGGAG 
AAGTACGGGC TGGAGAAGGA GAACCCCCGA GAGTACTGCA TCGCCCGGCA AGACGATAAG 
TCCGGCAAGG AGGCGATCCT GGACGATACC GAGTGTCCAC TGCAGATCTT CAGGGACTGG 
CCTGCAGACA GAGGAGCGCT AGTGTTCCAG CTGAAGAAGA GACCCCCGGA CTACCAGGGC 
AGGAAGGGGA GGAAAGTGGA CGACAAGGGC CTGCGAGGGA AGGACGGCAG CAGCTCCCTG 
CCGCCCGAGA AACTGCCTTA CCTGGTGGAG CTGAGCCCAG GGCGAGGGAA TCACTATGCC 
TACTACGCCT ATCGGCACCA CGAAGACGGG TCTGACTCAC GGGACAAACC AAAGCTGTAC 
CGGCTCCAGC ACAGCATCAC TGAGGTCGGC TCAGACTGCA CAGAGGACGG AGCCATTCAG 
CTGCTCGGTC CGGGGATTCT GCCCCACCAC TGCAACCTCA TGCACAGTGA AGGCATGGTG 
ACGGTGACGC CGCACGGCCC CGACGCCGAC ACCTTCGTCG ACGGGCAACG GGTCACTGAG 
ACGACGGTGC TGCGCTCGGG CTCCACCCTC CAGTTTGGCC AAGCGCACGT CTTCAAGTTT 
GTGGACCCGA TGTTCGACCA GGGAGGGAAG AGAGAGCCGG CCGCCATGAT GAGGAGTCGA 
CACAAGTCCG GCAGCGTACC CGAAACCACC TTCGACCTTC ACGGAGACGT CCACAGCGGA 
GCCGCGCTGC CTACCAGCAA GAGCTCAGGA AAGCTGGAGA TGGAGAGAGG AATGGTCAAA 
CCGATGATCA GAGGAGAGCA GCAGGACGGC AGGTCTCAGG ACATTTCAGC AGACAGAGCC 
GAGTTGACTC TGCCAGCCGG CATCGAGTTC AGAGACAACT CTGAAGACAC CTTCCTGTCG 
GCCATCATCA ACTACACCAA CAGCTCCACG GTTCACTTCA AACTCTCGCC CACGTACGTC 
CTGTACATGG CCTGCCGCTA CATCCTGTCG CCCTCGTACC GGCCCGACAT GTCGCCGTCC 
GAGAGGACGC ACAAGGTCAT CGCCATCGTC AACAAGATGG TGAGCATGAT GGAGGGAGTC 
ATCCAGGAGA TTCCTGAAGG GGATCAGAAG CAGAAGAACA TCGCGGGAGC GCTGGCTTTC 
TGGATGGCCA ACGCCTCCGA GCTGCTCAAC TTCATCAAGC AGGACCGAGA CCTGAACCGC 
ATCACGCTGG ACGCCCAGGA CATCCTCGCC CATCTGGTTC AGATGGCTTT CAAGTACCTG 
GTCCACTGTC TGCAGGCTGA TCTGAATAAC TACATGCCGG CCTTCCTGGA CGACCCCGAG 
GAACACAACC CACAGAGACC CAAGATCGAG GACGTCCTGC ACACCCTCAC GGGGGCGATG 
TCGTTACTGC GGCGGTGTCG GGTCAACGCC GCTCTCACCA TCCAGCTCTT CTCGCAGCTC 
TTCCACTTCA TCAACATGTG GCTCTTCAAC AAGCTGGTGA CGGACACCGA GTCCGGGCTG 
TGCTGTCACT ACTGGGGGGC CATCCTGCGG CAGCAGCTCA GCCACATCGA GGCCTGGGCG 
GAGAAACAGG GGCTGGAGCT CGCCGCCGAC TGCCACCTCA GTCGAATCGT GCAGGCCACC 
ACCCTGCTGA CCATGGACAA ATACTCCATG CAGGATGTGC CGAACATCCA CAACACCTGC 
TTCAAGCTCA ACTCGCTGCA GCTCCACGCC CTCATGAACA ACTACCACTG CGCTCCGGAC 
GAGCCGTACA TCCCGCCGGA GCTGATAGAC CACGTGGTGG CAGTGGCAGA GAACACAGCA 
GATGAGCTGG CGAGGAGCGA CGGCAGAGAG GTTCAGCTGG AGGAGGATCC GGACCTCCAG 
CTGCCGTTCC TCCTTCCCGA GGACGGTTAC TCCTGCGACG TGGTGCGCAG CCTCCCCAAC 
GGCCTGCAGG ACTTCCTGGA GCCGCTGCTG CAGAGAGGTT TCTGTCGGTT GGTACCTCAC 
CCTCGCTCTC CCGGTACCTG GACGGTCCAT TTTGAAGGAG CTGACTGCGA CAGCCACTTC 
TCTGCTGCTG ACAACTCAGA GATGCCAATG AGGAAAGAGC CGGAGGTTGT CACGGTAACC 
CTGAAGAAGC ACAACGGCAT GGGCCTCAGC ATCGTGGCTG CCAAGGGTGC TGGTCAGGAG 
AAGCTGGGCA TCTACATCAA GTCTGTCGTC AAAGGAGGAG CAGCTGATGT GGACGGGCGT 
CTGGCTGCAG GTGACCAGCT GTTGAGCGTG GACGGGCGGA GTCTGGTCGG CCTATCACAA 
GAGAGGGCAG CGGAGCTGAT GACGAGGACG GGCTCAGTCG TGACTCTGGA GGTCGCCAAG 
CAGGGAGCCA TTTACCACGG ACTGGCCACG CTCCTCAACC AGCCGAGCCC CATGATGCCA 
CGAGCGTCAG ACAGAGGCCG CGACAAGAAC GGGAAACTCC GACCAAAGAG CGAAGGCTTC 
GAGTTGTACA ACAGCTCGGT GCAGAACGGC TCTCCGGAGA GCCCGCAGGC CGGCTGGGAC 
TCTTACCCCG AACCAAAGAA GATGAGCGGC GAAGATCGAC TCCTGAAGAA CCGAGCCGAC 
CACCGCTCCA GCCCCAACGT CGCCAATCAG GGCCAGAGTC CTGCAGGCAA AGCAGTGTAC 
CCCGGAGGAC CTGGCACCAA GATCACCTCC GTCTCCACCG GCAACCTGTG CGCAGACGAT 
GAGCCCTCCC CTCCACGTCC AGAAGCTTAC CCCATCCCCA CCCAGACCTA CCCCAGGGAG 
TACTTCACCA TCCCGGCTTC CAAGAGCCAG GATCGGGTCG TTGGTCCTGG GCAGGGACAG 
CACTGGCAGG GCATGGAGGA CAGAGAACGC CTCCCTGTGG TCGACAACAT CCACAACAGC 
ATGCAGCGGG TTAACCACTC CCAGGAGGAG TTGTACCCTC CACCCGGAGG CCTGAGGCCG 
GAGGAGCGCA TGCAGCAGCA CTACCAGCAG GACTACCAGC ACCGCGACAT GGACTACCAG 
CACAGAGAGC TCGACTACCA GAGAGGAGGT CCACCAGGTG TCGGAGGTCC CGATCACTGG 
ACTCCACAGC CTCAGGTGTC CTCCTCGCTG GAGTCGTCCA CGTCCAGCCA GGAGCACCTC 
AACTTCTCCG CCTCCTCCTC GTCCTCCAAC AAAACCCAGA ACCAGAAGAC CGGGCCGGGC 
CGGTGGAAGA CTCCCAACGC CCCTCACTCC GTGCCGCCAC ATTCAGCCCA GCCGCCCTCC 
CGCTCCGACC TGCCCCCTCC TCCGCCTCCA CCGCCAGCTA CTTACCCCGA ATCCTACGAC 
CCATACGACC CCCAACCGGA CCTGCCGCTC CCGCCGCCTC CCTCCGCCGT CACTCCTGCG 
GTAGCTCAGC AGGCGGCCGA CCGCAAGAAA CGTGAGGAGC AGCAGCGCTG GTATGAGAAG 
GAGAAAGCTC GGCTGGAGGA GGAGAGGGAG AGGAAGAGGA GAGAGCAGGA GAGGAAGCTG 
GGTCAGATCC GGGCCAACCC CGTCATGCCC GTCCAGCCTC ACAACAACGG AGGATCCATG 
CCTCCGCCTC ACCACCAGCC GCCTCTGCCG TCTGCAGCCC CGCCTCAGCC CTACCTTCCC 
CCCGCTCAGC CCCAGCCTCC ACCATCCAGA CCGGAGAAGC TGACCAGCCT GCCGCGGTCA 
GCCGTTCCTC CACCCGCCAG CGCCTCCAAC CCTGCAGGCA TGGACACGGT GATCAGGGAG 
CTGTTGCCAC AGCAGCAGCC GCGGACCATC GAGAGGCGAG ACCTGCAGTA CATCACCATC 
AGCAAGGAGG AGCTCACCTC CAGCGACAGT CTGTCTCCAG ACCCCTGGAA GCGAGACGCC 
CGCGAGAAGG CCGAGAAGCA GCAGCAGCTC CACATCGTCG ACCTGCTGGA CAAGGAGATC 
CAGGAGCTCC AGGCGAAGCC GGAGCGGACG GCGGAGGAGA GCGACCGCCT CAGGAAGCTG 
ATGCTGGAGT GGCAGTTCCA GAAACGACTG CAGGAGTCCA AGCAGAGCGA CGAAGACGAG 
GAGGAGGAGG ACGACGAGGA CGTGGACACC ATGATGATCA TGCAGAGGCT GGAGGCCGAG 
AAGAGAGCCA GGCAGCAGAA TGCTGTCCCA GCTATCTCTG TTCTAGACTT GTTGCAGGAC 
GAGGAGCGGC GGCGTAAGCA GCAGCTGGAG GAGATTCGTA AGCGGGAGGC GGAGGAGCGA 
GCAAAGCAGG AGGAGGAGAG GAGGTGGAGG GAGGAGGAGA GAGCCAAGCG AGAGGCCGAG 
GAGAAGAGGA GACAGGAGGA GGAGTACTAC ACTCGCCTGG AGGCGGAGCG GCGCCGGCAG 
CACGACGAGG CCGAGAGGAA GCTGCTGACG CCCGACGACC CGGCCGGTCT GTATCGGCCC 
CCGCTGCCCC GGGATTACCA AACCCCGCCC CCCCAACAAA CCTATAAAAC CCCCCCGCCC 
CCCCCACAGA GAAACACCTC CTACCTCAAA ACGCAGGTCA CCTCCCCCGA CACGCTCTAC 
ACCGCCAAGT TTGTCTCCTA CGCGGGCGAC GAGGAGGAAG ACGAGGAGGC AGGGAGTGGC 
GGCGGCGGCG GCAGCAGCAG CCAATCAGGT CAGGTGAAGC TGTCGGCGAC CCGGAAGTCG 
TACGGCGACC TCCCGGCCTC CGCCTCGCCC AATCACAGCA GGCCTCAGCC GCCGCCCACT 
GCGCGCAAGC CCCGCCCTGT TTCTGACGGC ATCTTCCTGT CTGGCTCGTT CCAGCCGCCA 
TTCAACAACA ACTCCAACAG TACAGGTCCC CCCCTTCCTG CCAAACCCAG CTCCATCCCC 
CCGGGAGGCT TTAAAGGATT TAACTCGTAC ACTGGAAACT CAGCAGGAGT TGTAGGATCA 
GGAGAAGTCT ACAAGGACCC GCGAGACAAA TGGACGAAGA GTCAGGAGCA GGAGAACTCG 
AACCCCAGCG GCCCTCCGGA GAATCTGACC TTCAAGGAGC GGCAGCGTCT CTTCTCTCAG 
GGGAAGGAGG TTTCCAACAA GGTCAAAGCT TCGCGCAAAC TCATGGAGCT GGAGAACGAG 
CTCAACACCA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afdn ( XM_027284622.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_027140423.1 Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X2, mRNA.

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CloneID	OLa200704
Clone ID Related Accession (Same CDS sequence)	XM_027284622.1
Accession Version	XM_027284622.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5892bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	afadin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040021.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGCA GCAGGGAGGA GGAGAGGAGG AAACTGGCCG ACATCATCAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CGGCCCACAG AGGACCTGGA GTTCCATGGC 
GTGATGCGGT TCTACTTCCA GGACCGGGTC GCCGGTAACT TTGCCACCAA ATGCATCAGA 
GTGTCGAGCA CGGCGACAAC GCAGGACGTC ATCGAGACGT TAGCAGAGAA GTTCAGACCG 
GACATGAGGA TGCTCTCCTC GCCAAAATAC TCTTTGTACG AGGTCCACGT CAGTGGCGAA 
GAGCGTCAGC TGGATCTGGA CGAGAAGCCT CTGGTGGTTC AGCTGAACTG GAACAAAGAC 
GACAGAGAAG GACGCTTCGT CCTGAAGAAC GAGAACGACA TCCTCCCCAA GAAGAGTCAG 
AGTAACGGCC CGGAGAAGGA GAAGGACGGA GTGATCCAGA ACTTCAAGAG GACTTTGTCC 
AAGAAGGAGA AGAAGAAGGA GAAGAAGAGG GAGAAGGAGT TCGCCCGGAT CCCCGACGGA 
GACGAGCAGG CGCTCAGCCG AGAGGACGGG GAGAACAGTC GTCTGGCGGC GGAGGTTTAC 
AAGGACATGC CGGAGAACAG CTTCACCAGG ACGATCTCCA ACCCGGAGGT GGTGATGAAG 
AGGAGACGCC AGCAGAAGCT GGAGAAACGC ATGCAGGAGT TCATGAGCAG CGACGGGAGG 
CCCGACTCAG GGGGCACGTT GCGGATATAC GCCGACAGCC TGAAGCCGAA CATCCCGTAT 
AAAACCATCC TGCTGTCCAC GAGAGACACG GCTGACTTTG CGGTGGTGGA GGCGCTGGAG 
AAGTACGGGC TGGAGAAGGA GAACCCCCGA GAGTACTGCA TCGCCCGGCA AGACGATAAG 
TCCGGCAAGG AGGCGATCCT GGACGATACC GAGTGTCCAC TGCAGATCTT CAGGGACTGG 
CCTGCAGACA GAGGAGCGCT AGTGTTCCAG CTGAAGAAGA GACCCCCGGA CTACCAGGGC 
AGGAAGGGGA GGAAAGTGGA CGACAAGGGC CTGCGAGGGA AGGACGGCAG CAGCTCCCTG 
CCGCCCGAGA AACTGCCTTA CCTGGTGGAG CTGAGCCCAG GGCGAGGGAA TCACTATGCC 
TACTACGCCT ATCGGCACCA CGAAGACGGG TCTGACTCAC GGGACAAACC AAAGCTGTAC 
CGGCTCCAGC ACAGCATCAC TGAGGTCGGC TCAGACTGCA CAGAGGACGG AGCCATTCAG 
CTGCTCGGTC CGGGGATTCT GCCCCACCAC TGCAACCTCA TGCACAGTGA AGGCATGGTG 
ACGGTGACGC CGCACGGCCC CGACGCCGAC ACCTTCGTCG ACGGGCAACG GGTCACTGAG 
ACGACGGTGC TGCGCTCGGG CTCCACCCTC CAGTTTGGCC AAGCGCACGT CTTCAAGTTT 
GTGGACCCGA TGTTCGACCA GGGAGGGAAG AGAGAGCCGG CCGCCATGAT GAGGAGTCGA 
CACAAGTCCG GCAGCGTACC CGAAACCACC TTCGACCTTC ACGGAGACGT CCACAGCGGA 
GCCGCGCTGC CTACCAGCAA GAGCTCAGGA AAGCTGGAGA TGGAGAGAGG AATGGTCAAA 
CCGATGATCA GAGGAGAGCA GCAGGACGGC AGGTCTCAGG ACATTTCAGC AGACAGAGCC 
GAGTTGACTC TGCCAGCCGG CATCGAGTTC AGAGACAACT CTGAAGACAC CTTCCTGTCG 
GCCATCATCA ACTACACCAA CAGCTCCACG GTTCACTTCA AACTCTCGCC CACGTACGTC 
CTGTACATGG CCTGCCGCTA CATCCTGTCG CCCTCGTACC GGCCCGACAT GTCGCCGTCC 
GAGAGGACGC ACAAGGTCAT CGCCATCGTC AACAAGATGG TGAGCATGAT GGAGGGAGTC 
ATCCAGGAGA TTCCTGAAGG GGATCAGAAG CAGAAGAACA TCGCGGGAGC GCTGGCTTTC 
TGGATGGCCA ACGCCTCCGA GCTGCTCAAC TTCATCAAGC AGGACCGAGA CCTGAACCGC 
ATCACGCTGG ACGCCCAGGA CATCCTCGCC CATCTGGTTC AGATGGCTTT CAAGTACCTG 
GTCCACTGTC TGCAGGCTGA TCTGAATAAC TACATGCCGG CCTTCCTGGA CGACCCCGAG 
GAACACAACC CACAGAGACC CAAGATCGAG GACGTCCTGC ACACCCTCAC GGGGGCGATG 
TCGTTACTGC GGCGGTGTCG GGTCAACGCC GCTCTCACCA TCCAGCTCTT CTCGCAGCTC 
TTCCACTTCA TCAACATGTG GCTCTTCAAC AAGCTGGTGA CGGACACCGA GTCCGGGCTG 
TGCTGTCACT ACTGGGGGGC CATCCTGCGG CAGCAGCTCA GCCACATCGA GGCCTGGGCG 
GAGAAACAGG GGCTGGAGCT CGCCGCCGAC TGCCACCTCA GTCGAATCGT GCAGGCCACC 
ACCCTGCTGA CCATGGACAA ATACTCCATG CAGGATGTGC CGAACATCCA CAACACCTGC 
TTCAAGCTCA ACTCGCTGCA GCTCCACGCC CTCATGAACA ACTACCACTG CGCTCCGGAC 
GAGCCGTACA TCCCGCCGGA GCTGATAGAC CACGTGGTGG CAGTGGCAGA GAACACAGCA 
GATGAGCTGG CGAGGAGCGA CGGCAGAGAG GTTCAGCTGG AGGAGGATCC GGACCTCCAG 
CTGCCGTTCC TCCTTCCCGA GGACGGTTAC TCCTGCGACG TGGTGCGCAG CCTCCCCAAC 
GGCCTGCAGG ACTTCCTGGA GCCGCTGCTG CAGAGAGGTT TCTGTCGGTT GGTACCTCAC 
CCTCGCTCTC CCGGTACCTG GACGGTCCAT TTTGAAGGAG CTGACTGCGA CAGCCACTTC 
TCTGCTGCTG ACAACTCAGA GATGCCAATG AGGAAAGAGC CGGAGGTTGT CACGGTAACC 
CTGAAGAAGC ACAACGGCAT GGGCCTCAGC ATCGTGGCTG CCAAGGGTGC TGGTCAGGAG 
AAGCTGGGCA TCTACATCAA GTCTGTCGTC AAAGGAGGAG CAGCTGATGT GGACGGGCGT 
CTGGCTGCAG GTGACCAGCT GTTGAGCGTG GACGGGCGGA GTCTGGTCGG CCTATCACAA 
GAGAGGGCAG CGGAGCTGAT GACGAGGACG GGCTCAGTCG TGACTCTGGA GGTCGCCAAG 
CAGGGAGCCA TTTACCACGG ACTGGCCACG CTCCTCAACC AGCCGAGCCC CATGATGCCA 
CGAGCGTCAG ACAGAGGCCG CGACAAGAAC GGGAAACTCC GACCAAAGAG CGAAGGCTTC 
GAGTTGTACA ACAGCTCGGT GCAGAACGGC TCTCCGGAGA GCCCGCAGGC CGGCTGGGAC 
TCTTACCCCG AACCAAAGAA GATGAGCGGC GAAGATCGAC TCCTGAAGAA CCGAGCCGAC 
CACCGCTCCA GCCCCAACGT CGCCAATCAG GGCCAGAGTC CTGCAGGCAA AGCAGTGTAC 
CCCGGAGGAC CTGGCACCAA GATCACCTCC GTCTCCACCG GCAACCTGTG CGCAGACGAT 
GAGCCCTCCC CTCCACGTCC AGAAGCTTAC CCCATCCCCA CCCAGACCTA CCCCAGGGAG 
TACTTCACCA TCCCGGCTTC CAAGAGCCAG GATCGGGTCG TTGGTCCTGG GCAGGGACAG 
CACTGGCAGG GCATGGAGGA CAGAGAACGC CTCCCTGTGG TCGACAACAT CCACAACAGC 
ATGCAGCGGG TTAACCACTC CCAGGAGGAG TTGTACCCTC CACCCGGAGG CCTGAGGCCG 
GAGGAGCGCA TGCAGCAGCA CTACCAGCAG GACTACCAGC ACCGCGACAT GGACTACCAG 
CACAGAGAGC TCGACTACCA GAGAGGAGGT CCACCAGGTG TCGGAGGTCC CGATCACTGG 
ACTCCACAGC CTCAGGTGTC CTCCTCGCTG GAGTCGTCCA CGTCCAGCCA GGAGCACCTC 
AACTTCTCCG CCTCCTCCTC GTCCTCCAAC AAAACCCAGA ACCAGAAGAC CGGGCCGGGC 
CGGTGGAAGA CTCCCAACGC CCCTCACTCC GTGCCGCCAC ATTCAGCCCA GCCGCCCTCC 
CGCTCCGACC TGCCCCCTCC TCCGCCTCCA CCGCCAGCTA CTTACCCCGA ATCCTACGAC 
CCATACGACC CCCAACCGGA CCTGCCGCTC CCGCCGCCTC CCTCCGCCGT CACTCCTGCG 
GTAGCTCAGC AGGCGGCCGA CCGCAAGAAA CGTGAGGAGC AGCAGCGCTG GTATGAGAAG 
GAGAAAGCTC GGCTGGAGGA GGAGAGGGAG AGGAAGAGGA GAGAGCAGGA GAGGAAGCTG 
GGTCAGATCC GGGCCAACCC CGTCATGCCC GTCCAGCCTC ACAACAACGG AGGATCCATG 
CCTCCGCCTC ACCACCAGCC GCCTCTGCCG TCTGCAGCCC CGCCTCAGCC CTACCTTCCC 
CCCGCTCAGC CCCAGCCTCC ACCATCCAGA CCGGAGAAGC TGACCAGCCT GCCGCGGTCA 
GCCGTTCCTC CACCCGCCAG CGCCTCCAAC CCTGCAGGCA TGGACACGGT GATCAGGGAG 
CTGTTGCCAC AGCAGCAGCC GCGGACCATC GAGAGGCGAG ACCTGCAGTA CATCACCATC 
AGCAAGGAGG AGCTCACCTC CAGCGACAGT CTGTCTCCAG ACCCCTGGAA GCGAGACGCC 
CGCGAGAAGG CCGAGAAGCA GCAGCAGCTC CACATCGTCG ACCTGCTGGA CAAGGAGATC 
CAGGAGCTCC AGGCGAAGCC GGAGCGGACG GCGGAGGAGA GCGACCGCCT CAGGAAGCTG 
ATGCTGGAGT GGCAGTTCCA GAAACGACTG CAGGAGTCCA AGCAGAGCGA CGAAGACGAG 
GAGGAGGAGG ACGACGAGGA CGTGGACACC ATGATGATCA TGCAGAGGCT GGAGGCCGAG 
AAGAGAGCCA GGCAGAATGC TGTCCCAGCT ATCTCTGTTC TAGACTTGTT GCAGGACGAG 
GAGCGGCGGC GTAAGCAGCA GCTGGAGGAG ATTCGTAAGC GGGAGGCGGA GGAGCGAGCA 
AAGCAGGAGG AGGAGAGGAG GTGGAGGGAG GAGGAGAGAG CCAAGCGAGA GGCCGAGGAG 
AAGAGGAGAC AGGAGGAGGA GTACTACACT CGCCTGGAGG CGGAGCGGCG CCGGCAGCAC 
GACGAGGCCG AGAGGAAGCT GCTGACGCCC GACGACCCGG CCGGTCTGTA TCGGCCCCCG 
CTGCCCCGGG ATTACCAAAC CCCGCCCCCC CAACAAACCT ATAAAACCCC CCCGCCCCCC 
CCACAGAGAA ACACCTCCTA CCTCAAAACG CAGGTCACCT CCCCCGACAC GCTCTACACC 
GCCAAGTTTG TCTCCTACGC GGGCGACGAG GAGGAAGACG AGGAGGCAGG GAGTGGCGGC 
GGCGGCGGCA GCAGCAGCCA ATCAGGTCAG GTGAAGCTGT CGGCGACCCG GAAGTCGTAC 
GGCGACCTCC CGGCCTCCGC CTCGCCCAAT CACAGCAGGC CTCAGCCGCC GCCCACTGCG 
CGCAAGCCCC GCCCTGTTTC TGACGGCATC TTCCTGTCTG GCTCGTTCCA GCCGCCATTC 
AACAACAACT CCAACAGTAC AGGTCCCCCC CTTCCTGCCA AACCCAGCTC CATCCCCCCG 
GGAGGCTTTA AAGGATTTAA CTCGTACACT GGAAACTCAG CAGGAGTTGT AGGATCAGGA 
GAAGTCTACA AGGACCCGCG AGACAAATGG ACGAAGAGTC AGGAGCAGGA GAACTCGAAC 
CCCAGCGGCC CTCCGGAGAA TCTGACCTTC AAGGAGCGGC AGCGTCTCTT CTCTCAGGGG 
AAGGAGGTTT CCAACAAGGT CAAAGCTTCG CGCAAACTCA TGGAGCTGGA GAACGAGCTC 
AACACCAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027140423.1
CDS	233..6124
Translation	MSGSREEERRKLADIINHWNANRLDLFEISRPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERQLDLDEKPLVVQLNWNKDDREGRFVLKNENDILPKKSQSNGPEKEKDGVIQNFKRTLSKKEKKKEKKREKEFARIPDGDEQALSREDGENSRLAAEVYKDMPENSFTRTISNPEVVMKRRRQQKLEKRMQEFMSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTRDTADFAVVEALEKYGLEKENPREYCIARQDDKSGKEAILDDTECPLQIFRDWPADRGALVFQLKKRPPDYQGRKGRKVDDKGLRGKDGSSSLPPEKLPYLVELSPGRGNHYAYYAYRHHEDGSDSRDKPKLYRLQHSITEVGSDCTEDGAIQLLGPGILPHHCNLMHSEGMVTVTPHGPDADTFVDGQRVTETTVLRSGSTLQFGQAHVFKFVDPMFDQGGKREPAAMMRSRHKSGSVPETTFDLHGDVHSGAALPTSKSSGKLEMERGMVKPMIRGEQQDGRSQDISADRAELTLPAGIEFRDNSEDTFLSAIINYTNSSTVHFKLSPTYVLYMACRYILSPSYRPDMSPSERTHKVIAIVNKMVSMMEGVIQEIPEGDQKQKNIAGALAFWMANASELLNFIKQDRDLNRITLDAQDILAHLVQMAFKYLVHCLQADLNNYMPAFLDDPEEHNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVTDTESGLCCHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVPNIHNTCFKLNSLQLHALMNNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRSLPNGLQDFLEPLLQRGFCRLVPHPRSPGTWTVHFEGADCDSHFSAADNSEMPMRKEPEVVTVTLKKHNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRDKNGKLRPKSEGFELYNSSVQNGSPESPQAGWDSYPEPKKMSGEDRLLKNRADHRSSPNVANQGQSPAGKAVYPGGPGTKITSVSTGNLCADDEPSPPRPEAYPIPTQTYPREYFTIPASKSQDRVVGPGQGQHWQGMEDRERLPVVDNIHNSMQRVNHSQEELYPPPGGLRPEERMQQHYQQDYQHRDMDYQHRELDYQRGGPPGVGGPDHWTPQPQVSSSLESSTSSQEHLNFSASSSSSNKTQNQKTGPGRWKTPNAPHSVPPHSAQPPSRSDLPPPPPPPPATYPESYDPYDPQPDLPLPPPPSAVTPAVAQQAADRKKREEQQRWYEKEKARLEEERERKRREQERKLGQIRANPVMPVQPHNNGGSMPPPHHQPPLPSAAPPQPYLPPAQPQPPPSRPEKLTSLPRSAVPPPASASNPAGMDTVIRELLPQQQPRTIERRDLQYITISKEELTSSDSLSPDPWKRDAREKAEKQQQLHIVDLLDKEIQELQAKPERTAEESDRLRKLMLEWQFQKRLQESKQSDEDEEEEDDEDVDTMMIMQRLEAEKRARQNAVPAISVLDLLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERAKREAEEKRRQEEEYYTRLEAERRRQHDEAERKLLTPDDPAGLYRPPLPRDYQTPPPQQTYKTPPPPPQRNTSYLKTQVTSPDTLYTAKFVSYAGDEEEDEEAGSGGGGGSSSQSGQVKLSATRKSYGDLPASASPNHSRPQPPPTARKPRPVSDGIFLSGSFQPPFNNNSNSTGPPLPAKPSSIPPGGFKGFNSYTGNSAGVVGSGEVYKDPRDKWTKSQEQENSNPSGPPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNTK

Target ORF information:

RefSeq Version	XM_027284622.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027284622.1

ORF Insert Sequence:

ATGTCGGGCA GCAGGGAGGA GGAGAGGAGG AAACTGGCCG ACATCATCAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CGGCCCACAG AGGACCTGGA GTTCCATGGC 
GTGATGCGGT TCTACTTCCA GGACCGGGTC GCCGGTAACT TTGCCACCAA ATGCATCAGA 
GTGTCGAGCA CGGCGACAAC GCAGGACGTC ATCGAGACGT TAGCAGAGAA GTTCAGACCG 
GACATGAGGA TGCTCTCCTC GCCAAAATAC TCTTTGTACG AGGTCCACGT CAGTGGCGAA 
GAGCGTCAGC TGGATCTGGA CGAGAAGCCT CTGGTGGTTC AGCTGAACTG GAACAAAGAC 
GACAGAGAAG GACGCTTCGT CCTGAAGAAC GAGAACGACA TCCTCCCCAA GAAGAGTCAG 
AGTAACGGCC CGGAGAAGGA GAAGGACGGA GTGATCCAGA ACTTCAAGAG GACTTTGTCC 
AAGAAGGAGA AGAAGAAGGA GAAGAAGAGG GAGAAGGAGT TCGCCCGGAT CCCCGACGGA 
GACGAGCAGG CGCTCAGCCG AGAGGACGGG GAGAACAGTC GTCTGGCGGC GGAGGTTTAC 
AAGGACATGC CGGAGAACAG CTTCACCAGG ACGATCTCCA ACCCGGAGGT GGTGATGAAG 
AGGAGACGCC AGCAGAAGCT GGAGAAACGC ATGCAGGAGT TCATGAGCAG CGACGGGAGG 
CCCGACTCAG GGGGCACGTT GCGGATATAC GCCGACAGCC TGAAGCCGAA CATCCCGTAT 
AAAACCATCC TGCTGTCCAC GAGAGACACG GCTGACTTTG CGGTGGTGGA GGCGCTGGAG 
AAGTACGGGC TGGAGAAGGA GAACCCCCGA GAGTACTGCA TCGCCCGGCA AGACGATAAG 
TCCGGCAAGG AGGCGATCCT GGACGATACC GAGTGTCCAC TGCAGATCTT CAGGGACTGG 
CCTGCAGACA GAGGAGCGCT AGTGTTCCAG CTGAAGAAGA GACCCCCGGA CTACCAGGGC 
AGGAAGGGGA GGAAAGTGGA CGACAAGGGC CTGCGAGGGA AGGACGGCAG CAGCTCCCTG 
CCGCCCGAGA AACTGCCTTA CCTGGTGGAG CTGAGCCCAG GGCGAGGGAA TCACTATGCC 
TACTACGCCT ATCGGCACCA CGAAGACGGG TCTGACTCAC GGGACAAACC AAAGCTGTAC 
CGGCTCCAGC ACAGCATCAC TGAGGTCGGC TCAGACTGCA CAGAGGACGG AGCCATTCAG 
CTGCTCGGTC CGGGGATTCT GCCCCACCAC TGCAACCTCA TGCACAGTGA AGGCATGGTG 
ACGGTGACGC CGCACGGCCC CGACGCCGAC ACCTTCGTCG ACGGGCAACG GGTCACTGAG 
ACGACGGTGC TGCGCTCGGG CTCCACCCTC CAGTTTGGCC AAGCGCACGT CTTCAAGTTT 
GTGGACCCGA TGTTCGACCA GGGAGGGAAG AGAGAGCCGG CCGCCATGAT GAGGAGTCGA 
CACAAGTCCG GCAGCGTACC CGAAACCACC TTCGACCTTC ACGGAGACGT CCACAGCGGA 
GCCGCGCTGC CTACCAGCAA GAGCTCAGGA AAGCTGGAGA TGGAGAGAGG AATGGTCAAA 
CCGATGATCA GAGGAGAGCA GCAGGACGGC AGGTCTCAGG ACATTTCAGC AGACAGAGCC 
GAGTTGACTC TGCCAGCCGG CATCGAGTTC AGAGACAACT CTGAAGACAC CTTCCTGTCG 
GCCATCATCA ACTACACCAA CAGCTCCACG GTTCACTTCA AACTCTCGCC CACGTACGTC 
CTGTACATGG CCTGCCGCTA CATCCTGTCG CCCTCGTACC GGCCCGACAT GTCGCCGTCC 
GAGAGGACGC ACAAGGTCAT CGCCATCGTC AACAAGATGG TGAGCATGAT GGAGGGAGTC 
ATCCAGGAGA TTCCTGAAGG GGATCAGAAG CAGAAGAACA TCGCGGGAGC GCTGGCTTTC 
TGGATGGCCA ACGCCTCCGA GCTGCTCAAC TTCATCAAGC AGGACCGAGA CCTGAACCGC 
ATCACGCTGG ACGCCCAGGA CATCCTCGCC CATCTGGTTC AGATGGCTTT CAAGTACCTG 
GTCCACTGTC TGCAGGCTGA TCTGAATAAC TACATGCCGG CCTTCCTGGA CGACCCCGAG 
GAACACAACC CACAGAGACC CAAGATCGAG GACGTCCTGC ACACCCTCAC GGGGGCGATG 
TCGTTACTGC GGCGGTGTCG GGTCAACGCC GCTCTCACCA TCCAGCTCTT CTCGCAGCTC 
TTCCACTTCA TCAACATGTG GCTCTTCAAC AAGCTGGTGA CGGACACCGA GTCCGGGCTG 
TGCTGTCACT ACTGGGGGGC CATCCTGCGG CAGCAGCTCA GCCACATCGA GGCCTGGGCG 
GAGAAACAGG GGCTGGAGCT CGCCGCCGAC TGCCACCTCA GTCGAATCGT GCAGGCCACC 
ACCCTGCTGA CCATGGACAA ATACTCCATG CAGGATGTGC CGAACATCCA CAACACCTGC 
TTCAAGCTCA ACTCGCTGCA GCTCCACGCC CTCATGAACA ACTACCACTG CGCTCCGGAC 
GAGCCGTACA TCCCGCCGGA GCTGATAGAC CACGTGGTGG CAGTGGCAGA GAACACAGCA 
GATGAGCTGG CGAGGAGCGA CGGCAGAGAG GTTCAGCTGG AGGAGGATCC GGACCTCCAG 
CTGCCGTTCC TCCTTCCCGA GGACGGTTAC TCCTGCGACG TGGTGCGCAG CCTCCCCAAC 
GGCCTGCAGG ACTTCCTGGA GCCGCTGCTG CAGAGAGGTT TCTGTCGGTT GGTACCTCAC 
CCTCGCTCTC CCGGTACCTG GACGGTCCAT TTTGAAGGAG CTGACTGCGA CAGCCACTTC 
TCTGCTGCTG ACAACTCAGA GATGCCAATG AGGAAAGAGC CGGAGGTTGT CACGGTAACC 
CTGAAGAAGC ACAACGGCAT GGGCCTCAGC ATCGTGGCTG CCAAGGGTGC TGGTCAGGAG 
AAGCTGGGCA TCTACATCAA GTCTGTCGTC AAAGGAGGAG CAGCTGATGT GGACGGGCGT 
CTGGCTGCAG GTGACCAGCT GTTGAGCGTG GACGGGCGGA GTCTGGTCGG CCTATCACAA 
GAGAGGGCAG CGGAGCTGAT GACGAGGACG GGCTCAGTCG TGACTCTGGA GGTCGCCAAG 
CAGGGAGCCA TTTACCACGG ACTGGCCACG CTCCTCAACC AGCCGAGCCC CATGATGCCA 
CGAGCGTCAG ACAGAGGCCG CGACAAGAAC GGGAAACTCC GACCAAAGAG CGAAGGCTTC 
GAGTTGTACA ACAGCTCGGT GCAGAACGGC TCTCCGGAGA GCCCGCAGGC CGGCTGGGAC 
TCTTACCCCG AACCAAAGAA GATGAGCGGC GAAGATCGAC TCCTGAAGAA CCGAGCCGAC 
CACCGCTCCA GCCCCAACGT CGCCAATCAG GGCCAGAGTC CTGCAGGCAA AGCAGTGTAC 
CCCGGAGGAC CTGGCACCAA GATCACCTCC GTCTCCACCG GCAACCTGTG CGCAGACGAT 
GAGCCCTCCC CTCCACGTCC AGAAGCTTAC CCCATCCCCA CCCAGACCTA CCCCAGGGAG 
TACTTCACCA TCCCGGCTTC CAAGAGCCAG GATCGGGTCG TTGGTCCTGG GCAGGGACAG 
CACTGGCAGG GCATGGAGGA CAGAGAACGC CTCCCTGTGG TCGACAACAT CCACAACAGC 
ATGCAGCGGG TTAACCACTC CCAGGAGGAG TTGTACCCTC CACCCGGAGG CCTGAGGCCG 
GAGGAGCGCA TGCAGCAGCA CTACCAGCAG GACTACCAGC ACCGCGACAT GGACTACCAG 
CACAGAGAGC TCGACTACCA GAGAGGAGGT CCACCAGGTG TCGGAGGTCC CGATCACTGG 
ACTCCACAGC CTCAGGTGTC CTCCTCGCTG GAGTCGTCCA CGTCCAGCCA GGAGCACCTC 
AACTTCTCCG CCTCCTCCTC GTCCTCCAAC AAAACCCAGA ACCAGAAGAC CGGGCCGGGC 
CGGTGGAAGA CTCCCAACGC CCCTCACTCC GTGCCGCCAC ATTCAGCCCA GCCGCCCTCC 
CGCTCCGACC TGCCCCCTCC TCCGCCTCCA CCGCCAGCTA CTTACCCCGA ATCCTACGAC 
CCATACGACC CCCAACCGGA CCTGCCGCTC CCGCCGCCTC CCTCCGCCGT CACTCCTGCG 
GTAGCTCAGC AGGCGGCCGA CCGCAAGAAA CGTGAGGAGC AGCAGCGCTG GTATGAGAAG 
GAGAAAGCTC GGCTGGAGGA GGAGAGGGAG AGGAAGAGGA GAGAGCAGGA GAGGAAGCTG 
GGTCAGATCC GGGCCAACCC CGTCATGCCC GTCCAGCCTC ACAACAACGG AGGATCCATG 
CCTCCGCCTC ACCACCAGCC GCCTCTGCCG TCTGCAGCCC CGCCTCAGCC CTACCTTCCC 
CCCGCTCAGC CCCAGCCTCC ACCATCCAGA CCGGAGAAGC TGACCAGCCT GCCGCGGTCA 
GCCGTTCCTC CACCCGCCAG CGCCTCCAAC CCTGCAGGCA TGGACACGGT GATCAGGGAG 
CTGTTGCCAC AGCAGCAGCC GCGGACCATC GAGAGGCGAG ACCTGCAGTA CATCACCATC 
AGCAAGGAGG AGCTCACCTC CAGCGACAGT CTGTCTCCAG ACCCCTGGAA GCGAGACGCC 
CGCGAGAAGG CCGAGAAGCA GCAGCAGCTC CACATCGTCG ACCTGCTGGA CAAGGAGATC 
CAGGAGCTCC AGGCGAAGCC GGAGCGGACG GCGGAGGAGA GCGACCGCCT CAGGAAGCTG 
ATGCTGGAGT GGCAGTTCCA GAAACGACTG CAGGAGTCCA AGCAGAGCGA CGAAGACGAG 
GAGGAGGAGG ACGACGAGGA CGTGGACACC ATGATGATCA TGCAGAGGCT GGAGGCCGAG 
AAGAGAGCCA GGCAGAATGC TGTCCCAGCT ATCTCTGTTC TAGACTTGTT GCAGGACGAG 
GAGCGGCGGC GTAAGCAGCA GCTGGAGGAG ATTCGTAAGC GGGAGGCGGA GGAGCGAGCA 
AAGCAGGAGG AGGAGAGGAG GTGGAGGGAG GAGGAGAGAG CCAAGCGAGA GGCCGAGGAG 
AAGAGGAGAC AGGAGGAGGA GTACTACACT CGCCTGGAGG CGGAGCGGCG CCGGCAGCAC 
GACGAGGCCG AGAGGAAGCT GCTGACGCCC GACGACCCGG CCGGTCTGTA TCGGCCCCCG 
CTGCCCCGGG ATTACCAAAC CCCGCCCCCC CAACAAACCT ATAAAACCCC CCCGCCCCCC 
CCACAGAGAA ACACCTCCTA CCTCAAAACG CAGGTCACCT CCCCCGACAC GCTCTACACC 
GCCAAGTTTG TCTCCTACGC GGGCGACGAG GAGGAAGACG AGGAGGCAGG GAGTGGCGGC 
GGCGGCGGCA GCAGCAGCCA ATCAGGTCAG GTGAAGCTGT CGGCGACCCG GAAGTCGTAC 
GGCGACCTCC CGGCCTCCGC CTCGCCCAAT CACAGCAGGC CTCAGCCGCC GCCCACTGCG 
CGCAAGCCCC GCCCTGTTTC TGACGGCATC TTCCTGTCTG GCTCGTTCCA GCCGCCATTC 
AACAACAACT CCAACAGTAC AGGTCCCCCC CTTCCTGCCA AACCCAGCTC CATCCCCCCG 
GGAGGCTTTA AAGGATTTAA CTCGTACACT GGAAACTCAG CAGGAGTTGT AGGATCAGGA 
GAAGTCTACA AGGACCCGCG AGACAAATGG ACGAAGAGTC AGGAGCAGGA GAACTCGAAC 
CCCAGCGGCC CTCCGGAGAA TCTGACCTTC AAGGAGCGGC AGCGTCTCTT CTCTCAGGGG 
AAGGAGGTTT CCAACAAGGT CAAAGCTTCG CGCAAACTCA TGGAGCTGGA GAACGAGCTC 
AACACCAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afdn ( XM_027284623.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_027140424.1 Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X3, mRNA.

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CloneID	OLa200705
Clone ID Related Accession (Same CDS sequence)	XM_027284623.1
Accession Version	XM_027284623.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5892bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	afadin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040021.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGCA GCAGGGAGGA GGAGAGGAGG AAACTGGCCG ACATCATCAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CGGCCCACAG AGGACCTGGA GTTCCATGGC 
GTGATGCGGT TCTACTTCCA GGACCGGGTC GCCGGTAACT TTGCCACCAA ATGCATCAGA 
GTGTCGAGCA CGGCGACAAC GCAGGACGTC ATCGAGACGT TAGCAGAGAA GTTCAGACCG 
GACATGAGGA TGCTCTCCTC GCCAAAATAC TCTTTGTACG AGGTCCACGT CAGTGGCGAA 
GAGCGTCAGC TGGATCTGGA CGAGAAGCCT CTGGTGGTTC AGCTGAACTG GAACAAAGAC 
GACAGAGAAG GACGCTTCGT CCTGAAGAAC GAGAACGACA TCCTCCCCAA GAGTCAGAGT 
AACGGCCCGG AGAAGGAGAA GGACGGAGTG ATCCAGAACT TCAAGAGGAC TTTGTCCAAG 
AAGGAGAAGA AGAAGGAGAA GAAGAGGGAG AAGGAGTTCG CCCGGATCCC CGACGGAGAC 
GAGCAGGCGC TCAGCCGAGA GGACGGGGAG AACAGTCGTC TGGCGGCGGA GGTTTACAAG 
GACATGCCGG AGAACAGCTT CACCAGGACG ATCTCCAACC CGGAGGTGGT GATGAAGAGG 
AGACGCCAGC AGAAGCTGGA GAAACGCATG CAGGAGTTCA TGAGCAGCGA CGGGAGGCCC 
GACTCAGGGG GCACGTTGCG GATATACGCC GACAGCCTGA AGCCGAACAT CCCGTATAAA 
ACCATCCTGC TGTCCACGAG AGACACGGCT GACTTTGCGG TGGTGGAGGC GCTGGAGAAG 
TACGGGCTGG AGAAGGAGAA CCCCCGAGAG TACTGCATCG CCCGGCAAGA CGATAAGTCC 
GGCAAGGAGG CGATCCTGGA CGATACCGAG TGTCCACTGC AGATCTTCAG GGACTGGCCT 
GCAGACAGAG GAGCGCTAGT GTTCCAGCTG AAGAAGAGAC CCCCGGACTA CCAGGGCAGG 
AAGGGGAGGA AAGTGGACGA CAAGGGCCTG CGAGGGAAGG ACGGCAGCAG CTCCCTGCCG 
CCCGAGAAAC TGCCTTACCT GGTGGAGCTG AGCCCAGGGC GAGGGAATCA CTATGCCTAC 
TACGCCTATC GGCACCACGA AGACGGGTCT GACTCACGGG ACAAACCAAA GCTGTACCGG 
CTCCAGCACA GCATCACTGA GGTCGGCTCA GACTGCACAG AGGACGGAGC CATTCAGCTG 
CTCGGTCCGG GGATTCTGCC CCACCACTGC AACCTCATGC ACAGTGAAGG CATGGTGACG 
GTGACGCCGC ACGGCCCCGA CGCCGACACC TTCGTCGACG GGCAACGGGT CACTGAGACG 
ACGGTGCTGC GCTCGGGCTC CACCCTCCAG TTTGGCCAAG CGCACGTCTT CAAGTTTGTG 
GACCCGATGT TCGACCAGGG AGGGAAGAGA GAGCCGGCCG CCATGATGAG GAGTCGACAC 
AAGTCCGGCA GCGTACCCGA AACCACCTTC GACCTTCACG GAGACGTCCA CAGCGGAGCC 
GCGCTGCCTA CCAGCAAGAG CTCAGGAAAG CTGGAGATGG AGAGAGGAAT GGTCAAACCG 
ATGATCAGAG GAGAGCAGCA GGACGGCAGG TCTCAGGACA TTTCAGCAGA CAGAGCCGAG 
TTGACTCTGC CAGCCGGCAT CGAGTTCAGA GACAACTCTG AAGACACCTT CCTGTCGGCC 
ATCATCAACT ACACCAACAG CTCCACGGTT CACTTCAAAC TCTCGCCCAC GTACGTCCTG 
TACATGGCCT GCCGCTACAT CCTGTCGCCC TCGTACCGGC CCGACATGTC GCCGTCCGAG 
AGGACGCACA AGGTCATCGC CATCGTCAAC AAGATGGTGA GCATGATGGA GGGAGTCATC 
CAGGAGATTC CTGAAGGGGA TCAGAAGCAG AAGAACATCG CGGGAGCGCT GGCTTTCTGG 
ATGGCCAACG CCTCCGAGCT GCTCAACTTC ATCAAGCAGG ACCGAGACCT GAACCGCATC 
ACGCTGGACG CCCAGGACAT CCTCGCCCAT CTGGTTCAGA TGGCTTTCAA GTACCTGGTC 
CACTGTCTGC AGGCTGATCT GAATAACTAC ATGCCGGCCT TCCTGGACGA CCCCGAGGAA 
CACAACCCAC AGAGACCCAA GATCGAGGAC GTCCTGCACA CCCTCACGGG GGCGATGTCG 
TTACTGCGGC GGTGTCGGGT CAACGCCGCT CTCACCATCC AGCTCTTCTC GCAGCTCTTC 
CACTTCATCA ACATGTGGCT CTTCAACAAG CTGGTGACGG ACACCGAGTC CGGGCTGTGC 
TGTCACTACT GGGGGGCCAT CCTGCGGCAG CAGCTCAGCC ACATCGAGGC CTGGGCGGAG 
AAACAGGGGC TGGAGCTCGC CGCCGACTGC CACCTCAGTC GAATCGTGCA GGCCACCACC 
CTGCTGACCA TGGACAAATA CTCCATGCAG GATGTGCCGA ACATCCACAA CACCTGCTTC 
AAGCTCAACT CGCTGCAGCT CCACGCCCTC ATGAACAACT ACCACTGCGC TCCGGACGAG 
CCGTACATCC CGCCGGAGCT GATAGACCAC GTGGTGGCAG TGGCAGAGAA CACAGCAGAT 
GAGCTGGCGA GGAGCGACGG CAGAGAGGTT CAGCTGGAGG AGGATCCGGA CCTCCAGCTG 
CCGTTCCTCC TTCCCGAGGA CGGTTACTCC TGCGACGTGG TGCGCAGCCT CCCCAACGGC 
CTGCAGGACT TCCTGGAGCC GCTGCTGCAG AGAGGTTTCT GTCGGTTGGT ACCTCACCCT 
CGCTCTCCCG GTACCTGGAC GGTCCATTTT GAAGGAGCTG ACTGCGACAG CCACTTCTCT 
GCTGCTGACA ACTCAGAGAT GCCAATGAGG AAAGAGCCGG AGGTTGTCAC GGTAACCCTG 
AAGAAGCACA ACGGCATGGG CCTCAGCATC GTGGCTGCCA AGGGTGCTGG TCAGGAGAAG 
CTGGGCATCT ACATCAAGTC TGTCGTCAAA GGAGGAGCAG CTGATGTGGA CGGGCGTCTG 
GCTGCAGGTG ACCAGCTGTT GAGCGTGGAC GGGCGGAGTC TGGTCGGCCT ATCACAAGAG 
AGGGCAGCGG AGCTGATGAC GAGGACGGGC TCAGTCGTGA CTCTGGAGGT CGCCAAGCAG 
GGAGCCATTT ACCACGGACT GGCCACGCTC CTCAACCAGC CGAGCCCCAT GATGCCACGA 
GCGTCAGACA GAGGCCGCGA CAAGAACGGG AAACTCCGAC CAAAGAGCGA AGGCTTCGAG 
TTGTACAACA GCTCGGTGCA GAACGGCTCT CCGGAGAGCC CGCAGGCCGG CTGGGACTCT 
TACCCCGAAC CAAAGAAGAT GAGCGGCGAA GATCGACTCC TGAAGAACCG AGCCGACCAC 
CGCTCCAGCC CCAACGTCGC CAATCAGGGC CAGAGTCCTG CAGGCAAAGC AGTGTACCCC 
GGAGGACCTG GCACCAAGAT CACCTCCGTC TCCACCGGCA ACCTGTGCGC AGACGATGAG 
CCCTCCCCTC CACGTCCAGA AGCTTACCCC ATCCCCACCC AGACCTACCC CAGGGAGTAC 
TTCACCATCC CGGCTTCCAA GAGCCAGGAT CGGGTCGTTG GTCCTGGGCA GGGACAGCAC 
TGGCAGGGCA TGGAGGACAG AGAACGCCTC CCTGTGGTCG ACAACATCCA CAACAGCATG 
CAGCGGGTTA ACCACTCCCA GGAGGAGTTG TACCCTCCAC CCGGAGGCCT GAGGCCGGAG 
GAGCGCATGC AGCAGCACTA CCAGCAGGAC TACCAGCACC GCGACATGGA CTACCAGCAC 
AGAGAGCTCG ACTACCAGAG AGGAGGTCCA CCAGGTGTCG GAGGTCCCGA TCACTGGACT 
CCACAGCCTC AGGTGTCCTC CTCGCTGGAG TCGTCCACGT CCAGCCAGGA GCACCTCAAC 
TTCTCCGCCT CCTCCTCGTC CTCCAACAAA ACCCAGAACC AGAAGACCGG GCCGGGCCGG 
TGGAAGACTC CCAACGCCCC TCACTCCGTG CCGCCACATT CAGCCCAGCC GCCCTCCCGC 
TCCGACCTGC CCCCTCCTCC GCCTCCACCG CCAGCTACTT ACCCCGAATC CTACGACCCA 
TACGACCCCC AACCGGACCT GCCGCTCCCG CCGCCTCCCT CCGCCGTCAC TCCTGCGGTA 
GCTCAGCAGG CGGCCGACCG CAAGAAACGT GAGGAGCAGC AGCGCTGGTA TGAGAAGGAG 
AAAGCTCGGC TGGAGGAGGA GAGGGAGAGG AAGAGGAGAG AGCAGGAGAG GAAGCTGGGT 
CAGATCCGGG CCAACCCCGT CATGCCCGTC CAGCCTCACA ACAACGGAGG ATCCATGCCT 
CCGCCTCACC ACCAGCCGCC TCTGCCGTCT GCAGCCCCGC CTCAGCCCTA CCTTCCCCCC 
GCTCAGCCCC AGCCTCCACC ATCCAGACCG GAGAAGCTGA CCAGCCTGCC GCGGTCAGCC 
GTTCCTCCAC CCGCCAGCGC CTCCAACCCT GCAGGCATGG ACACGGTGAT CAGGGAGCTG 
TTGCCACAGC AGCAGCCGCG GACCATCGAG AGGCGAGACC TGCAGTACAT CACCATCAGC 
AAGGAGGAGC TCACCTCCAG CGACAGTCTG TCTCCAGACC CCTGGAAGCG AGACGCCCGC 
GAGAAGGCCG AGAAGCAGCA GCAGCTCCAC ATCGTCGACC TGCTGGACAA GGAGATCCAG 
GAGCTCCAGG CGAAGCCGGA GCGGACGGCG GAGGAGAGCG ACCGCCTCAG GAAGCTGATG 
CTGGAGTGGC AGTTCCAGAA ACGACTGCAG GAGTCCAAGC AGAGCGACGA AGACGAGGAG 
GAGGAGGACG ACGAGGACGT GGACACCATG ATGATCATGC AGAGGCTGGA GGCCGAGAAG 
AGAGCCAGGC AGCAGAATGC TGTCCCAGCT ATCTCTGTTC TAGACTTGTT GCAGGACGAG 
GAGCGGCGGC GTAAGCAGCA GCTGGAGGAG ATTCGTAAGC GGGAGGCGGA GGAGCGAGCA 
AAGCAGGAGG AGGAGAGGAG GTGGAGGGAG GAGGAGAGAG CCAAGCGAGA GGCCGAGGAG 
AAGAGGAGAC AGGAGGAGGA GTACTACACT CGCCTGGAGG CGGAGCGGCG CCGGCAGCAC 
GACGAGGCCG AGAGGAAGCT GCTGACGCCC GACGACCCGG CCGGTCTGTA TCGGCCCCCG 
CTGCCCCGGG ATTACCAAAC CCCGCCCCCC CAACAAACCT ATAAAACCCC CCCGCCCCCC 
CCACAGAGAA ACACCTCCTA CCTCAAAACG CAGGTCACCT CCCCCGACAC GCTCTACACC 
GCCAAGTTTG TCTCCTACGC GGGCGACGAG GAGGAAGACG AGGAGGCAGG GAGTGGCGGC 
GGCGGCGGCA GCAGCAGCCA ATCAGGTCAG GTGAAGCTGT CGGCGACCCG GAAGTCGTAC 
GGCGACCTCC CGGCCTCCGC CTCGCCCAAT CACAGCAGGC CTCAGCCGCC GCCCACTGCG 
CGCAAGCCCC GCCCTGTTTC TGACGGCATC TTCCTGTCTG GCTCGTTCCA GCCGCCATTC 
AACAACAACT CCAACAGTAC AGGTCCCCCC CTTCCTGCCA AACCCAGCTC CATCCCCCCG 
GGAGGCTTTA AAGGATTTAA CTCGTACACT GGAAACTCAG CAGGAGTTGT AGGATCAGGA 
GAAGTCTACA AGGACCCGCG AGACAAATGG ACGAAGAGTC AGGAGCAGGA GAACTCGAAC 
CCCAGCGGCC CTCCGGAGAA TCTGACCTTC AAGGAGCGGC AGCGTCTCTT CTCTCAGGGG 
AAGGAGGTTT CCAACAAGGT CAAAGCTTCG CGCAAACTCA TGGAGCTGGA GAACGAGCTC 
AACACCAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027140424.1
CDS	233..6124
Translation	MSGSREEERRKLADIINHWNANRLDLFEISRPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERQLDLDEKPLVVQLNWNKDDREGRFVLKNENDILPKSQSNGPEKEKDGVIQNFKRTLSKKEKKKEKKREKEFARIPDGDEQALSREDGENSRLAAEVYKDMPENSFTRTISNPEVVMKRRRQQKLEKRMQEFMSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTRDTADFAVVEALEKYGLEKENPREYCIARQDDKSGKEAILDDTECPLQIFRDWPADRGALVFQLKKRPPDYQGRKGRKVDDKGLRGKDGSSSLPPEKLPYLVELSPGRGNHYAYYAYRHHEDGSDSRDKPKLYRLQHSITEVGSDCTEDGAIQLLGPGILPHHCNLMHSEGMVTVTPHGPDADTFVDGQRVTETTVLRSGSTLQFGQAHVFKFVDPMFDQGGKREPAAMMRSRHKSGSVPETTFDLHGDVHSGAALPTSKSSGKLEMERGMVKPMIRGEQQDGRSQDISADRAELTLPAGIEFRDNSEDTFLSAIINYTNSSTVHFKLSPTYVLYMACRYILSPSYRPDMSPSERTHKVIAIVNKMVSMMEGVIQEIPEGDQKQKNIAGALAFWMANASELLNFIKQDRDLNRITLDAQDILAHLVQMAFKYLVHCLQADLNNYMPAFLDDPEEHNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVTDTESGLCCHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVPNIHNTCFKLNSLQLHALMNNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRSLPNGLQDFLEPLLQRGFCRLVPHPRSPGTWTVHFEGADCDSHFSAADNSEMPMRKEPEVVTVTLKKHNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRDKNGKLRPKSEGFELYNSSVQNGSPESPQAGWDSYPEPKKMSGEDRLLKNRADHRSSPNVANQGQSPAGKAVYPGGPGTKITSVSTGNLCADDEPSPPRPEAYPIPTQTYPREYFTIPASKSQDRVVGPGQGQHWQGMEDRERLPVVDNIHNSMQRVNHSQEELYPPPGGLRPEERMQQHYQQDYQHRDMDYQHRELDYQRGGPPGVGGPDHWTPQPQVSSSLESSTSSQEHLNFSASSSSSNKTQNQKTGPGRWKTPNAPHSVPPHSAQPPSRSDLPPPPPPPPATYPESYDPYDPQPDLPLPPPPSAVTPAVAQQAADRKKREEQQRWYEKEKARLEEERERKRREQERKLGQIRANPVMPVQPHNNGGSMPPPHHQPPLPSAAPPQPYLPPAQPQPPPSRPEKLTSLPRSAVPPPASASNPAGMDTVIRELLPQQQPRTIERRDLQYITISKEELTSSDSLSPDPWKRDAREKAEKQQQLHIVDLLDKEIQELQAKPERTAEESDRLRKLMLEWQFQKRLQESKQSDEDEEEEDDEDVDTMMIMQRLEAEKRARQQNAVPAISVLDLLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERAKREAEEKRRQEEEYYTRLEAERRRQHDEAERKLLTPDDPAGLYRPPLPRDYQTPPPQQTYKTPPPPPQRNTSYLKTQVTSPDTLYTAKFVSYAGDEEEDEEAGSGGGGGSSSQSGQVKLSATRKSYGDLPASASPNHSRPQPPPTARKPRPVSDGIFLSGSFQPPFNNNSNSTGPPLPAKPSSIPPGGFKGFNSYTGNSAGVVGSGEVYKDPRDKWTKSQEQENSNPSGPPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNTK

Target ORF information:

RefSeq Version	XM_027284623.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027284623.1

ORF Insert Sequence:

ATGTCGGGCA GCAGGGAGGA GGAGAGGAGG AAACTGGCCG ACATCATCAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CGGCCCACAG AGGACCTGGA GTTCCATGGC 
GTGATGCGGT TCTACTTCCA GGACCGGGTC GCCGGTAACT TTGCCACCAA ATGCATCAGA 
GTGTCGAGCA CGGCGACAAC GCAGGACGTC ATCGAGACGT TAGCAGAGAA GTTCAGACCG 
GACATGAGGA TGCTCTCCTC GCCAAAATAC TCTTTGTACG AGGTCCACGT CAGTGGCGAA 
GAGCGTCAGC TGGATCTGGA CGAGAAGCCT CTGGTGGTTC AGCTGAACTG GAACAAAGAC 
GACAGAGAAG GACGCTTCGT CCTGAAGAAC GAGAACGACA TCCTCCCCAA GAGTCAGAGT 
AACGGCCCGG AGAAGGAGAA GGACGGAGTG ATCCAGAACT TCAAGAGGAC TTTGTCCAAG 
AAGGAGAAGA AGAAGGAGAA GAAGAGGGAG AAGGAGTTCG CCCGGATCCC CGACGGAGAC 
GAGCAGGCGC TCAGCCGAGA GGACGGGGAG AACAGTCGTC TGGCGGCGGA GGTTTACAAG 
GACATGCCGG AGAACAGCTT CACCAGGACG ATCTCCAACC CGGAGGTGGT GATGAAGAGG 
AGACGCCAGC AGAAGCTGGA GAAACGCATG CAGGAGTTCA TGAGCAGCGA CGGGAGGCCC 
GACTCAGGGG GCACGTTGCG GATATACGCC GACAGCCTGA AGCCGAACAT CCCGTATAAA 
ACCATCCTGC TGTCCACGAG AGACACGGCT GACTTTGCGG TGGTGGAGGC GCTGGAGAAG 
TACGGGCTGG AGAAGGAGAA CCCCCGAGAG TACTGCATCG CCCGGCAAGA CGATAAGTCC 
GGCAAGGAGG CGATCCTGGA CGATACCGAG TGTCCACTGC AGATCTTCAG GGACTGGCCT 
GCAGACAGAG GAGCGCTAGT GTTCCAGCTG AAGAAGAGAC CCCCGGACTA CCAGGGCAGG 
AAGGGGAGGA AAGTGGACGA CAAGGGCCTG CGAGGGAAGG ACGGCAGCAG CTCCCTGCCG 
CCCGAGAAAC TGCCTTACCT GGTGGAGCTG AGCCCAGGGC GAGGGAATCA CTATGCCTAC 
TACGCCTATC GGCACCACGA AGACGGGTCT GACTCACGGG ACAAACCAAA GCTGTACCGG 
CTCCAGCACA GCATCACTGA GGTCGGCTCA GACTGCACAG AGGACGGAGC CATTCAGCTG 
CTCGGTCCGG GGATTCTGCC CCACCACTGC AACCTCATGC ACAGTGAAGG CATGGTGACG 
GTGACGCCGC ACGGCCCCGA CGCCGACACC TTCGTCGACG GGCAACGGGT CACTGAGACG 
ACGGTGCTGC GCTCGGGCTC CACCCTCCAG TTTGGCCAAG CGCACGTCTT CAAGTTTGTG 
GACCCGATGT TCGACCAGGG AGGGAAGAGA GAGCCGGCCG CCATGATGAG GAGTCGACAC 
AAGTCCGGCA GCGTACCCGA AACCACCTTC GACCTTCACG GAGACGTCCA CAGCGGAGCC 
GCGCTGCCTA CCAGCAAGAG CTCAGGAAAG CTGGAGATGG AGAGAGGAAT GGTCAAACCG 
ATGATCAGAG GAGAGCAGCA GGACGGCAGG TCTCAGGACA TTTCAGCAGA CAGAGCCGAG 
TTGACTCTGC CAGCCGGCAT CGAGTTCAGA GACAACTCTG AAGACACCTT CCTGTCGGCC 
ATCATCAACT ACACCAACAG CTCCACGGTT CACTTCAAAC TCTCGCCCAC GTACGTCCTG 
TACATGGCCT GCCGCTACAT CCTGTCGCCC TCGTACCGGC CCGACATGTC GCCGTCCGAG 
AGGACGCACA AGGTCATCGC CATCGTCAAC AAGATGGTGA GCATGATGGA GGGAGTCATC 
CAGGAGATTC CTGAAGGGGA TCAGAAGCAG AAGAACATCG CGGGAGCGCT GGCTTTCTGG 
ATGGCCAACG CCTCCGAGCT GCTCAACTTC ATCAAGCAGG ACCGAGACCT GAACCGCATC 
ACGCTGGACG CCCAGGACAT CCTCGCCCAT CTGGTTCAGA TGGCTTTCAA GTACCTGGTC 
CACTGTCTGC AGGCTGATCT GAATAACTAC ATGCCGGCCT TCCTGGACGA CCCCGAGGAA 
CACAACCCAC AGAGACCCAA GATCGAGGAC GTCCTGCACA CCCTCACGGG GGCGATGTCG 
TTACTGCGGC GGTGTCGGGT CAACGCCGCT CTCACCATCC AGCTCTTCTC GCAGCTCTTC 
CACTTCATCA ACATGTGGCT CTTCAACAAG CTGGTGACGG ACACCGAGTC CGGGCTGTGC 
TGTCACTACT GGGGGGCCAT CCTGCGGCAG CAGCTCAGCC ACATCGAGGC CTGGGCGGAG 
AAACAGGGGC TGGAGCTCGC CGCCGACTGC CACCTCAGTC GAATCGTGCA GGCCACCACC 
CTGCTGACCA TGGACAAATA CTCCATGCAG GATGTGCCGA ACATCCACAA CACCTGCTTC 
AAGCTCAACT CGCTGCAGCT CCACGCCCTC ATGAACAACT ACCACTGCGC TCCGGACGAG 
CCGTACATCC CGCCGGAGCT GATAGACCAC GTGGTGGCAG TGGCAGAGAA CACAGCAGAT 
GAGCTGGCGA GGAGCGACGG CAGAGAGGTT CAGCTGGAGG AGGATCCGGA CCTCCAGCTG 
CCGTTCCTCC TTCCCGAGGA CGGTTACTCC TGCGACGTGG TGCGCAGCCT CCCCAACGGC 
CTGCAGGACT TCCTGGAGCC GCTGCTGCAG AGAGGTTTCT GTCGGTTGGT ACCTCACCCT 
CGCTCTCCCG GTACCTGGAC GGTCCATTTT GAAGGAGCTG ACTGCGACAG CCACTTCTCT 
GCTGCTGACA ACTCAGAGAT GCCAATGAGG AAAGAGCCGG AGGTTGTCAC GGTAACCCTG 
AAGAAGCACA ACGGCATGGG CCTCAGCATC GTGGCTGCCA AGGGTGCTGG TCAGGAGAAG 
CTGGGCATCT ACATCAAGTC TGTCGTCAAA GGAGGAGCAG CTGATGTGGA CGGGCGTCTG 
GCTGCAGGTG ACCAGCTGTT GAGCGTGGAC GGGCGGAGTC TGGTCGGCCT ATCACAAGAG 
AGGGCAGCGG AGCTGATGAC GAGGACGGGC TCAGTCGTGA CTCTGGAGGT CGCCAAGCAG 
GGAGCCATTT ACCACGGACT GGCCACGCTC CTCAACCAGC CGAGCCCCAT GATGCCACGA 
GCGTCAGACA GAGGCCGCGA CAAGAACGGG AAACTCCGAC CAAAGAGCGA AGGCTTCGAG 
TTGTACAACA GCTCGGTGCA GAACGGCTCT CCGGAGAGCC CGCAGGCCGG CTGGGACTCT 
TACCCCGAAC CAAAGAAGAT GAGCGGCGAA GATCGACTCC TGAAGAACCG AGCCGACCAC 
CGCTCCAGCC CCAACGTCGC CAATCAGGGC CAGAGTCCTG CAGGCAAAGC AGTGTACCCC 
GGAGGACCTG GCACCAAGAT CACCTCCGTC TCCACCGGCA ACCTGTGCGC AGACGATGAG 
CCCTCCCCTC CACGTCCAGA AGCTTACCCC ATCCCCACCC AGACCTACCC CAGGGAGTAC 
TTCACCATCC CGGCTTCCAA GAGCCAGGAT CGGGTCGTTG GTCCTGGGCA GGGACAGCAC 
TGGCAGGGCA TGGAGGACAG AGAACGCCTC CCTGTGGTCG ACAACATCCA CAACAGCATG 
CAGCGGGTTA ACCACTCCCA GGAGGAGTTG TACCCTCCAC CCGGAGGCCT GAGGCCGGAG 
GAGCGCATGC AGCAGCACTA CCAGCAGGAC TACCAGCACC GCGACATGGA CTACCAGCAC 
AGAGAGCTCG ACTACCAGAG AGGAGGTCCA CCAGGTGTCG GAGGTCCCGA TCACTGGACT 
CCACAGCCTC AGGTGTCCTC CTCGCTGGAG TCGTCCACGT CCAGCCAGGA GCACCTCAAC 
TTCTCCGCCT CCTCCTCGTC CTCCAACAAA ACCCAGAACC AGAAGACCGG GCCGGGCCGG 
TGGAAGACTC CCAACGCCCC TCACTCCGTG CCGCCACATT CAGCCCAGCC GCCCTCCCGC 
TCCGACCTGC CCCCTCCTCC GCCTCCACCG CCAGCTACTT ACCCCGAATC CTACGACCCA 
TACGACCCCC AACCGGACCT GCCGCTCCCG CCGCCTCCCT CCGCCGTCAC TCCTGCGGTA 
GCTCAGCAGG CGGCCGACCG CAAGAAACGT GAGGAGCAGC AGCGCTGGTA TGAGAAGGAG 
AAAGCTCGGC TGGAGGAGGA GAGGGAGAGG AAGAGGAGAG AGCAGGAGAG GAAGCTGGGT 
CAGATCCGGG CCAACCCCGT CATGCCCGTC CAGCCTCACA ACAACGGAGG ATCCATGCCT 
CCGCCTCACC ACCAGCCGCC TCTGCCGTCT GCAGCCCCGC CTCAGCCCTA CCTTCCCCCC 
GCTCAGCCCC AGCCTCCACC ATCCAGACCG GAGAAGCTGA CCAGCCTGCC GCGGTCAGCC 
GTTCCTCCAC CCGCCAGCGC CTCCAACCCT GCAGGCATGG ACACGGTGAT CAGGGAGCTG 
TTGCCACAGC AGCAGCCGCG GACCATCGAG AGGCGAGACC TGCAGTACAT CACCATCAGC 
AAGGAGGAGC TCACCTCCAG CGACAGTCTG TCTCCAGACC CCTGGAAGCG AGACGCCCGC 
GAGAAGGCCG AGAAGCAGCA GCAGCTCCAC ATCGTCGACC TGCTGGACAA GGAGATCCAG 
GAGCTCCAGG CGAAGCCGGA GCGGACGGCG GAGGAGAGCG ACCGCCTCAG GAAGCTGATG 
CTGGAGTGGC AGTTCCAGAA ACGACTGCAG GAGTCCAAGC AGAGCGACGA AGACGAGGAG 
GAGGAGGACG ACGAGGACGT GGACACCATG ATGATCATGC AGAGGCTGGA GGCCGAGAAG 
AGAGCCAGGC AGCAGAATGC TGTCCCAGCT ATCTCTGTTC TAGACTTGTT GCAGGACGAG 
GAGCGGCGGC GTAAGCAGCA GCTGGAGGAG ATTCGTAAGC GGGAGGCGGA GGAGCGAGCA 
AAGCAGGAGG AGGAGAGGAG GTGGAGGGAG GAGGAGAGAG CCAAGCGAGA GGCCGAGGAG 
AAGAGGAGAC AGGAGGAGGA GTACTACACT CGCCTGGAGG CGGAGCGGCG CCGGCAGCAC 
GACGAGGCCG AGAGGAAGCT GCTGACGCCC GACGACCCGG CCGGTCTGTA TCGGCCCCCG 
CTGCCCCGGG ATTACCAAAC CCCGCCCCCC CAACAAACCT ATAAAACCCC CCCGCCCCCC 
CCACAGAGAA ACACCTCCTA CCTCAAAACG CAGGTCACCT CCCCCGACAC GCTCTACACC 
GCCAAGTTTG TCTCCTACGC GGGCGACGAG GAGGAAGACG AGGAGGCAGG GAGTGGCGGC 
GGCGGCGGCA GCAGCAGCCA ATCAGGTCAG GTGAAGCTGT CGGCGACCCG GAAGTCGTAC 
GGCGACCTCC CGGCCTCCGC CTCGCCCAAT CACAGCAGGC CTCAGCCGCC GCCCACTGCG 
CGCAAGCCCC GCCCTGTTTC TGACGGCATC TTCCTGTCTG GCTCGTTCCA GCCGCCATTC 
AACAACAACT CCAACAGTAC AGGTCCCCCC CTTCCTGCCA AACCCAGCTC CATCCCCCCG 
GGAGGCTTTA AAGGATTTAA CTCGTACACT GGAAACTCAG CAGGAGTTGT AGGATCAGGA 
GAAGTCTACA AGGACCCGCG AGACAAATGG ACGAAGAGTC AGGAGCAGGA GAACTCGAAC 
CCCAGCGGCC CTCCGGAGAA TCTGACCTTC AAGGAGCGGC AGCGTCTCTT CTCTCAGGGG 
AAGGAGGTTT CCAACAAGGT CAAAGCTTCG CGCAAACTCA TGGAGCTGGA GAACGAGCTC 
AACACCAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afdn ( XM_027284624.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_027140425.1 Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X4, mRNA.

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CloneID	OLa200706
Clone ID Related Accession (Same CDS sequence)	XM_027284624.1
Accession Version	XM_027284624.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5892bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	afadin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040021.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGCA GCAGGGAGGA GGAGAGGAGG AAACTGGCCG ACATCATCAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CGGCCCACAG AGGACCTGGA GTTCCATGGC 
GTGATGCGGT TCTACTTCCA GGACCGGGTC GCCGGTAACT TTGCCACCAA ATGCATCAGA 
GTGTCGAGCA CGGCGACAAC GCAGGACGTC ATCGAGACGT TAGCAGAGAA GTTCAGACCG 
GACATGAGGA TGCTCTCCTC GCCAAAATAC TCTTTGTACG AGGTCCACGT CAGTGGCGAG 
CGTCAGCTGG ATCTGGACGA GAAGCCTCTG GTGGTTCAGC TGAACTGGAA CAAAGACGAC 
AGAGAAGGAC GCTTCGTCCT GAAGAACGAG AACGACATCC TCCCCAAGAA GAGTCAGAGT 
AACGGCCCGG AGAAGGAGAA GGACGGAGTG ATCCAGAACT TCAAGAGGAC TTTGTCCAAG 
AAGGAGAAGA AGAAGGAGAA GAAGAGGGAG AAGGAGTTCG CCCGGATCCC CGACGGAGAC 
GAGCAGGCGC TCAGCCGAGA GGACGGGGAG AACAGTCGTC TGGCGGCGGA GGTTTACAAG 
GACATGCCGG AGAACAGCTT CACCAGGACG ATCTCCAACC CGGAGGTGGT GATGAAGAGG 
AGACGCCAGC AGAAGCTGGA GAAACGCATG CAGGAGTTCA TGAGCAGCGA CGGGAGGCCC 
GACTCAGGGG GCACGTTGCG GATATACGCC GACAGCCTGA AGCCGAACAT CCCGTATAAA 
ACCATCCTGC TGTCCACGAG AGACACGGCT GACTTTGCGG TGGTGGAGGC GCTGGAGAAG 
TACGGGCTGG AGAAGGAGAA CCCCCGAGAG TACTGCATCG CCCGGCAAGA CGATAAGTCC 
GGCAAGGAGG CGATCCTGGA CGATACCGAG TGTCCACTGC AGATCTTCAG GGACTGGCCT 
GCAGACAGAG GAGCGCTAGT GTTCCAGCTG AAGAAGAGAC CCCCGGACTA CCAGGGCAGG 
AAGGGGAGGA AAGTGGACGA CAAGGGCCTG CGAGGGAAGG ACGGCAGCAG CTCCCTGCCG 
CCCGAGAAAC TGCCTTACCT GGTGGAGCTG AGCCCAGGGC GAGGGAATCA CTATGCCTAC 
TACGCCTATC GGCACCACGA AGACGGGTCT GACTCACGGG ACAAACCAAA GCTGTACCGG 
CTCCAGCACA GCATCACTGA GGTCGGCTCA GACTGCACAG AGGACGGAGC CATTCAGCTG 
CTCGGTCCGG GGATTCTGCC CCACCACTGC AACCTCATGC ACAGTGAAGG CATGGTGACG 
GTGACGCCGC ACGGCCCCGA CGCCGACACC TTCGTCGACG GGCAACGGGT CACTGAGACG 
ACGGTGCTGC GCTCGGGCTC CACCCTCCAG TTTGGCCAAG CGCACGTCTT CAAGTTTGTG 
GACCCGATGT TCGACCAGGG AGGGAAGAGA GAGCCGGCCG CCATGATGAG GAGTCGACAC 
AAGTCCGGCA GCGTACCCGA AACCACCTTC GACCTTCACG GAGACGTCCA CAGCGGAGCC 
GCGCTGCCTA CCAGCAAGAG CTCAGGAAAG CTGGAGATGG AGAGAGGAAT GGTCAAACCG 
ATGATCAGAG GAGAGCAGCA GGACGGCAGG TCTCAGGACA TTTCAGCAGA CAGAGCCGAG 
TTGACTCTGC CAGCCGGCAT CGAGTTCAGA GACAACTCTG AAGACACCTT CCTGTCGGCC 
ATCATCAACT ACACCAACAG CTCCACGGTT CACTTCAAAC TCTCGCCCAC GTACGTCCTG 
TACATGGCCT GCCGCTACAT CCTGTCGCCC TCGTACCGGC CCGACATGTC GCCGTCCGAG 
AGGACGCACA AGGTCATCGC CATCGTCAAC AAGATGGTGA GCATGATGGA GGGAGTCATC 
CAGGAGATTC CTGAAGGGGA TCAGAAGCAG AAGAACATCG CGGGAGCGCT GGCTTTCTGG 
ATGGCCAACG CCTCCGAGCT GCTCAACTTC ATCAAGCAGG ACCGAGACCT GAACCGCATC 
ACGCTGGACG CCCAGGACAT CCTCGCCCAT CTGGTTCAGA TGGCTTTCAA GTACCTGGTC 
CACTGTCTGC AGGCTGATCT GAATAACTAC ATGCCGGCCT TCCTGGACGA CCCCGAGGAA 
CACAACCCAC AGAGACCCAA GATCGAGGAC GTCCTGCACA CCCTCACGGG GGCGATGTCG 
TTACTGCGGC GGTGTCGGGT CAACGCCGCT CTCACCATCC AGCTCTTCTC GCAGCTCTTC 
CACTTCATCA ACATGTGGCT CTTCAACAAG CTGGTGACGG ACACCGAGTC CGGGCTGTGC 
TGTCACTACT GGGGGGCCAT CCTGCGGCAG CAGCTCAGCC ACATCGAGGC CTGGGCGGAG 
AAACAGGGGC TGGAGCTCGC CGCCGACTGC CACCTCAGTC GAATCGTGCA GGCCACCACC 
CTGCTGACCA TGGACAAATA CTCCATGCAG GATGTGCCGA ACATCCACAA CACCTGCTTC 
AAGCTCAACT CGCTGCAGCT CCACGCCCTC ATGAACAACT ACCACTGCGC TCCGGACGAG 
CCGTACATCC CGCCGGAGCT GATAGACCAC GTGGTGGCAG TGGCAGAGAA CACAGCAGAT 
GAGCTGGCGA GGAGCGACGG CAGAGAGGTT CAGCTGGAGG AGGATCCGGA CCTCCAGCTG 
CCGTTCCTCC TTCCCGAGGA CGGTTACTCC TGCGACGTGG TGCGCAGCCT CCCCAACGGC 
CTGCAGGACT TCCTGGAGCC GCTGCTGCAG AGAGGTTTCT GTCGGTTGGT ACCTCACCCT 
CGCTCTCCCG GTACCTGGAC GGTCCATTTT GAAGGAGCTG ACTGCGACAG CCACTTCTCT 
GCTGCTGACA ACTCAGAGAT GCCAATGAGG AAAGAGCCGG AGGTTGTCAC GGTAACCCTG 
AAGAAGCACA ACGGCATGGG CCTCAGCATC GTGGCTGCCA AGGGTGCTGG TCAGGAGAAG 
CTGGGCATCT ACATCAAGTC TGTCGTCAAA GGAGGAGCAG CTGATGTGGA CGGGCGTCTG 
GCTGCAGGTG ACCAGCTGTT GAGCGTGGAC GGGCGGAGTC TGGTCGGCCT ATCACAAGAG 
AGGGCAGCGG AGCTGATGAC GAGGACGGGC TCAGTCGTGA CTCTGGAGGT CGCCAAGCAG 
GGAGCCATTT ACCACGGACT GGCCACGCTC CTCAACCAGC CGAGCCCCAT GATGCCACGA 
GCGTCAGACA GAGGCCGCGA CAAGAACGGG AAACTCCGAC CAAAGAGCGA AGGCTTCGAG 
TTGTACAACA GCTCGGTGCA GAACGGCTCT CCGGAGAGCC CGCAGGCCGG CTGGGACTCT 
TACCCCGAAC CAAAGAAGAT GAGCGGCGAA GATCGACTCC TGAAGAACCG AGCCGACCAC 
CGCTCCAGCC CCAACGTCGC CAATCAGGGC CAGAGTCCTG CAGGCAAAGC AGTGTACCCC 
GGAGGACCTG GCACCAAGAT CACCTCCGTC TCCACCGGCA ACCTGTGCGC AGACGATGAG 
CCCTCCCCTC CACGTCCAGA AGCTTACCCC ATCCCCACCC AGACCTACCC CAGGGAGTAC 
TTCACCATCC CGGCTTCCAA GAGCCAGGAT CGGGTCGTTG GTCCTGGGCA GGGACAGCAC 
TGGCAGGGCA TGGAGGACAG AGAACGCCTC CCTGTGGTCG ACAACATCCA CAACAGCATG 
CAGCGGGTTA ACCACTCCCA GGAGGAGTTG TACCCTCCAC CCGGAGGCCT GAGGCCGGAG 
GAGCGCATGC AGCAGCACTA CCAGCAGGAC TACCAGCACC GCGACATGGA CTACCAGCAC 
AGAGAGCTCG ACTACCAGAG AGGAGGTCCA CCAGGTGTCG GAGGTCCCGA TCACTGGACT 
CCACAGCCTC AGGTGTCCTC CTCGCTGGAG TCGTCCACGT CCAGCCAGGA GCACCTCAAC 
TTCTCCGCCT CCTCCTCGTC CTCCAACAAA ACCCAGAACC AGAAGACCGG GCCGGGCCGG 
TGGAAGACTC CCAACGCCCC TCACTCCGTG CCGCCACATT CAGCCCAGCC GCCCTCCCGC 
TCCGACCTGC CCCCTCCTCC GCCTCCACCG CCAGCTACTT ACCCCGAATC CTACGACCCA 
TACGACCCCC AACCGGACCT GCCGCTCCCG CCGCCTCCCT CCGCCGTCAC TCCTGCGGTA 
GCTCAGCAGG CGGCCGACCG CAAGAAACGT GAGGAGCAGC AGCGCTGGTA TGAGAAGGAG 
AAAGCTCGGC TGGAGGAGGA GAGGGAGAGG AAGAGGAGAG AGCAGGAGAG GAAGCTGGGT 
CAGATCCGGG CCAACCCCGT CATGCCCGTC CAGCCTCACA ACAACGGAGG ATCCATGCCT 
CCGCCTCACC ACCAGCCGCC TCTGCCGTCT GCAGCCCCGC CTCAGCCCTA CCTTCCCCCC 
GCTCAGCCCC AGCCTCCACC ATCCAGACCG GAGAAGCTGA CCAGCCTGCC GCGGTCAGCC 
GTTCCTCCAC CCGCCAGCGC CTCCAACCCT GCAGGCATGG ACACGGTGAT CAGGGAGCTG 
TTGCCACAGC AGCAGCCGCG GACCATCGAG AGGCGAGACC TGCAGTACAT CACCATCAGC 
AAGGAGGAGC TCACCTCCAG CGACAGTCTG TCTCCAGACC CCTGGAAGCG AGACGCCCGC 
GAGAAGGCCG AGAAGCAGCA GCAGCTCCAC ATCGTCGACC TGCTGGACAA GGAGATCCAG 
GAGCTCCAGG CGAAGCCGGA GCGGACGGCG GAGGAGAGCG ACCGCCTCAG GAAGCTGATG 
CTGGAGTGGC AGTTCCAGAA ACGACTGCAG GAGTCCAAGC AGAGCGACGA AGACGAGGAG 
GAGGAGGACG ACGAGGACGT GGACACCATG ATGATCATGC AGAGGCTGGA GGCCGAGAAG 
AGAGCCAGGC AGCAGAATGC TGTCCCAGCT ATCTCTGTTC TAGACTTGTT GCAGGACGAG 
GAGCGGCGGC GTAAGCAGCA GCTGGAGGAG ATTCGTAAGC GGGAGGCGGA GGAGCGAGCA 
AAGCAGGAGG AGGAGAGGAG GTGGAGGGAG GAGGAGAGAG CCAAGCGAGA GGCCGAGGAG 
AAGAGGAGAC AGGAGGAGGA GTACTACACT CGCCTGGAGG CGGAGCGGCG CCGGCAGCAC 
GACGAGGCCG AGAGGAAGCT GCTGACGCCC GACGACCCGG CCGGTCTGTA TCGGCCCCCG 
CTGCCCCGGG ATTACCAAAC CCCGCCCCCC CAACAAACCT ATAAAACCCC CCCGCCCCCC 
CCACAGAGAA ACACCTCCTA CCTCAAAACG CAGGTCACCT CCCCCGACAC GCTCTACACC 
GCCAAGTTTG TCTCCTACGC GGGCGACGAG GAGGAAGACG AGGAGGCAGG GAGTGGCGGC 
GGCGGCGGCA GCAGCAGCCA ATCAGGTCAG GTGAAGCTGT CGGCGACCCG GAAGTCGTAC 
GGCGACCTCC CGGCCTCCGC CTCGCCCAAT CACAGCAGGC CTCAGCCGCC GCCCACTGCG 
CGCAAGCCCC GCCCTGTTTC TGACGGCATC TTCCTGTCTG GCTCGTTCCA GCCGCCATTC 
AACAACAACT CCAACAGTAC AGGTCCCCCC CTTCCTGCCA AACCCAGCTC CATCCCCCCG 
GGAGGCTTTA AAGGATTTAA CTCGTACACT GGAAACTCAG CAGGAGTTGT AGGATCAGGA 
GAAGTCTACA AGGACCCGCG AGACAAATGG ACGAAGAGTC AGGAGCAGGA GAACTCGAAC 
CCCAGCGGCC CTCCGGAGAA TCTGACCTTC AAGGAGCGGC AGCGTCTCTT CTCTCAGGGG 
AAGGAGGTTT CCAACAAGGT CAAAGCTTCG CGCAAACTCA TGGAGCTGGA GAACGAGCTC 
AACACCAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027140425.1
CDS	233..6124
Translation	MSGSREEERRKLADIINHWNANRLDLFEISRPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERQLDLDEKPLVVQLNWNKDDREGRFVLKNENDILPKKSQSNGPEKEKDGVIQNFKRTLSKKEKKKEKKREKEFARIPDGDEQALSREDGENSRLAAEVYKDMPENSFTRTISNPEVVMKRRRQQKLEKRMQEFMSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTRDTADFAVVEALEKYGLEKENPREYCIARQDDKSGKEAILDDTECPLQIFRDWPADRGALVFQLKKRPPDYQGRKGRKVDDKGLRGKDGSSSLPPEKLPYLVELSPGRGNHYAYYAYRHHEDGSDSRDKPKLYRLQHSITEVGSDCTEDGAIQLLGPGILPHHCNLMHSEGMVTVTPHGPDADTFVDGQRVTETTVLRSGSTLQFGQAHVFKFVDPMFDQGGKREPAAMMRSRHKSGSVPETTFDLHGDVHSGAALPTSKSSGKLEMERGMVKPMIRGEQQDGRSQDISADRAELTLPAGIEFRDNSEDTFLSAIINYTNSSTVHFKLSPTYVLYMACRYILSPSYRPDMSPSERTHKVIAIVNKMVSMMEGVIQEIPEGDQKQKNIAGALAFWMANASELLNFIKQDRDLNRITLDAQDILAHLVQMAFKYLVHCLQADLNNYMPAFLDDPEEHNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVTDTESGLCCHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVPNIHNTCFKLNSLQLHALMNNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRSLPNGLQDFLEPLLQRGFCRLVPHPRSPGTWTVHFEGADCDSHFSAADNSEMPMRKEPEVVTVTLKKHNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRDKNGKLRPKSEGFELYNSSVQNGSPESPQAGWDSYPEPKKMSGEDRLLKNRADHRSSPNVANQGQSPAGKAVYPGGPGTKITSVSTGNLCADDEPSPPRPEAYPIPTQTYPREYFTIPASKSQDRVVGPGQGQHWQGMEDRERLPVVDNIHNSMQRVNHSQEELYPPPGGLRPEERMQQHYQQDYQHRDMDYQHRELDYQRGGPPGVGGPDHWTPQPQVSSSLESSTSSQEHLNFSASSSSSNKTQNQKTGPGRWKTPNAPHSVPPHSAQPPSRSDLPPPPPPPPATYPESYDPYDPQPDLPLPPPPSAVTPAVAQQAADRKKREEQQRWYEKEKARLEEERERKRREQERKLGQIRANPVMPVQPHNNGGSMPPPHHQPPLPSAAPPQPYLPPAQPQPPPSRPEKLTSLPRSAVPPPASASNPAGMDTVIRELLPQQQPRTIERRDLQYITISKEELTSSDSLSPDPWKRDAREKAEKQQQLHIVDLLDKEIQELQAKPERTAEESDRLRKLMLEWQFQKRLQESKQSDEDEEEEDDEDVDTMMIMQRLEAEKRARQQNAVPAISVLDLLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERAKREAEEKRRQEEEYYTRLEAERRRQHDEAERKLLTPDDPAGLYRPPLPRDYQTPPPQQTYKTPPPPPQRNTSYLKTQVTSPDTLYTAKFVSYAGDEEEDEEAGSGGGGGSSSQSGQVKLSATRKSYGDLPASASPNHSRPQPPPTARKPRPVSDGIFLSGSFQPPFNNNSNSTGPPLPAKPSSIPPGGFKGFNSYTGNSAGVVGSGEVYKDPRDKWTKSQEQENSNPSGPPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNTK

Target ORF information:

RefSeq Version	XM_027284624.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027284624.1

ORF Insert Sequence:

ATGTCGGGCA GCAGGGAGGA GGAGAGGAGG AAACTGGCCG ACATCATCAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CGGCCCACAG AGGACCTGGA GTTCCATGGC 
GTGATGCGGT TCTACTTCCA GGACCGGGTC GCCGGTAACT TTGCCACCAA ATGCATCAGA 
GTGTCGAGCA CGGCGACAAC GCAGGACGTC ATCGAGACGT TAGCAGAGAA GTTCAGACCG 
GACATGAGGA TGCTCTCCTC GCCAAAATAC TCTTTGTACG AGGTCCACGT CAGTGGCGAG 
CGTCAGCTGG ATCTGGACGA GAAGCCTCTG GTGGTTCAGC TGAACTGGAA CAAAGACGAC 
AGAGAAGGAC GCTTCGTCCT GAAGAACGAG AACGACATCC TCCCCAAGAA GAGTCAGAGT 
AACGGCCCGG AGAAGGAGAA GGACGGAGTG ATCCAGAACT TCAAGAGGAC TTTGTCCAAG 
AAGGAGAAGA AGAAGGAGAA GAAGAGGGAG AAGGAGTTCG CCCGGATCCC CGACGGAGAC 
GAGCAGGCGC TCAGCCGAGA GGACGGGGAG AACAGTCGTC TGGCGGCGGA GGTTTACAAG 
GACATGCCGG AGAACAGCTT CACCAGGACG ATCTCCAACC CGGAGGTGGT GATGAAGAGG 
AGACGCCAGC AGAAGCTGGA GAAACGCATG CAGGAGTTCA TGAGCAGCGA CGGGAGGCCC 
GACTCAGGGG GCACGTTGCG GATATACGCC GACAGCCTGA AGCCGAACAT CCCGTATAAA 
ACCATCCTGC TGTCCACGAG AGACACGGCT GACTTTGCGG TGGTGGAGGC GCTGGAGAAG 
TACGGGCTGG AGAAGGAGAA CCCCCGAGAG TACTGCATCG CCCGGCAAGA CGATAAGTCC 
GGCAAGGAGG CGATCCTGGA CGATACCGAG TGTCCACTGC AGATCTTCAG GGACTGGCCT 
GCAGACAGAG GAGCGCTAGT GTTCCAGCTG AAGAAGAGAC CCCCGGACTA CCAGGGCAGG 
AAGGGGAGGA AAGTGGACGA CAAGGGCCTG CGAGGGAAGG ACGGCAGCAG CTCCCTGCCG 
CCCGAGAAAC TGCCTTACCT GGTGGAGCTG AGCCCAGGGC GAGGGAATCA CTATGCCTAC 
TACGCCTATC GGCACCACGA AGACGGGTCT GACTCACGGG ACAAACCAAA GCTGTACCGG 
CTCCAGCACA GCATCACTGA GGTCGGCTCA GACTGCACAG AGGACGGAGC CATTCAGCTG 
CTCGGTCCGG GGATTCTGCC CCACCACTGC AACCTCATGC ACAGTGAAGG CATGGTGACG 
GTGACGCCGC ACGGCCCCGA CGCCGACACC TTCGTCGACG GGCAACGGGT CACTGAGACG 
ACGGTGCTGC GCTCGGGCTC CACCCTCCAG TTTGGCCAAG CGCACGTCTT CAAGTTTGTG 
GACCCGATGT TCGACCAGGG AGGGAAGAGA GAGCCGGCCG CCATGATGAG GAGTCGACAC 
AAGTCCGGCA GCGTACCCGA AACCACCTTC GACCTTCACG GAGACGTCCA CAGCGGAGCC 
GCGCTGCCTA CCAGCAAGAG CTCAGGAAAG CTGGAGATGG AGAGAGGAAT GGTCAAACCG 
ATGATCAGAG GAGAGCAGCA GGACGGCAGG TCTCAGGACA TTTCAGCAGA CAGAGCCGAG 
TTGACTCTGC CAGCCGGCAT CGAGTTCAGA GACAACTCTG AAGACACCTT CCTGTCGGCC 
ATCATCAACT ACACCAACAG CTCCACGGTT CACTTCAAAC TCTCGCCCAC GTACGTCCTG 
TACATGGCCT GCCGCTACAT CCTGTCGCCC TCGTACCGGC CCGACATGTC GCCGTCCGAG 
AGGACGCACA AGGTCATCGC CATCGTCAAC AAGATGGTGA GCATGATGGA GGGAGTCATC 
CAGGAGATTC CTGAAGGGGA TCAGAAGCAG AAGAACATCG CGGGAGCGCT GGCTTTCTGG 
ATGGCCAACG CCTCCGAGCT GCTCAACTTC ATCAAGCAGG ACCGAGACCT GAACCGCATC 
ACGCTGGACG CCCAGGACAT CCTCGCCCAT CTGGTTCAGA TGGCTTTCAA GTACCTGGTC 
CACTGTCTGC AGGCTGATCT GAATAACTAC ATGCCGGCCT TCCTGGACGA CCCCGAGGAA 
CACAACCCAC AGAGACCCAA GATCGAGGAC GTCCTGCACA CCCTCACGGG GGCGATGTCG 
TTACTGCGGC GGTGTCGGGT CAACGCCGCT CTCACCATCC AGCTCTTCTC GCAGCTCTTC 
CACTTCATCA ACATGTGGCT CTTCAACAAG CTGGTGACGG ACACCGAGTC CGGGCTGTGC 
TGTCACTACT GGGGGGCCAT CCTGCGGCAG CAGCTCAGCC ACATCGAGGC CTGGGCGGAG 
AAACAGGGGC TGGAGCTCGC CGCCGACTGC CACCTCAGTC GAATCGTGCA GGCCACCACC 
CTGCTGACCA TGGACAAATA CTCCATGCAG GATGTGCCGA ACATCCACAA CACCTGCTTC 
AAGCTCAACT CGCTGCAGCT CCACGCCCTC ATGAACAACT ACCACTGCGC TCCGGACGAG 
CCGTACATCC CGCCGGAGCT GATAGACCAC GTGGTGGCAG TGGCAGAGAA CACAGCAGAT 
GAGCTGGCGA GGAGCGACGG CAGAGAGGTT CAGCTGGAGG AGGATCCGGA CCTCCAGCTG 
CCGTTCCTCC TTCCCGAGGA CGGTTACTCC TGCGACGTGG TGCGCAGCCT CCCCAACGGC 
CTGCAGGACT TCCTGGAGCC GCTGCTGCAG AGAGGTTTCT GTCGGTTGGT ACCTCACCCT 
CGCTCTCCCG GTACCTGGAC GGTCCATTTT GAAGGAGCTG ACTGCGACAG CCACTTCTCT 
GCTGCTGACA ACTCAGAGAT GCCAATGAGG AAAGAGCCGG AGGTTGTCAC GGTAACCCTG 
AAGAAGCACA ACGGCATGGG CCTCAGCATC GTGGCTGCCA AGGGTGCTGG TCAGGAGAAG 
CTGGGCATCT ACATCAAGTC TGTCGTCAAA GGAGGAGCAG CTGATGTGGA CGGGCGTCTG 
GCTGCAGGTG ACCAGCTGTT GAGCGTGGAC GGGCGGAGTC TGGTCGGCCT ATCACAAGAG 
AGGGCAGCGG AGCTGATGAC GAGGACGGGC TCAGTCGTGA CTCTGGAGGT CGCCAAGCAG 
GGAGCCATTT ACCACGGACT GGCCACGCTC CTCAACCAGC CGAGCCCCAT GATGCCACGA 
GCGTCAGACA GAGGCCGCGA CAAGAACGGG AAACTCCGAC CAAAGAGCGA AGGCTTCGAG 
TTGTACAACA GCTCGGTGCA GAACGGCTCT CCGGAGAGCC CGCAGGCCGG CTGGGACTCT 
TACCCCGAAC CAAAGAAGAT GAGCGGCGAA GATCGACTCC TGAAGAACCG AGCCGACCAC 
CGCTCCAGCC CCAACGTCGC CAATCAGGGC CAGAGTCCTG CAGGCAAAGC AGTGTACCCC 
GGAGGACCTG GCACCAAGAT CACCTCCGTC TCCACCGGCA ACCTGTGCGC AGACGATGAG 
CCCTCCCCTC CACGTCCAGA AGCTTACCCC ATCCCCACCC AGACCTACCC CAGGGAGTAC 
TTCACCATCC CGGCTTCCAA GAGCCAGGAT CGGGTCGTTG GTCCTGGGCA GGGACAGCAC 
TGGCAGGGCA TGGAGGACAG AGAACGCCTC CCTGTGGTCG ACAACATCCA CAACAGCATG 
CAGCGGGTTA ACCACTCCCA GGAGGAGTTG TACCCTCCAC CCGGAGGCCT GAGGCCGGAG 
GAGCGCATGC AGCAGCACTA CCAGCAGGAC TACCAGCACC GCGACATGGA CTACCAGCAC 
AGAGAGCTCG ACTACCAGAG AGGAGGTCCA CCAGGTGTCG GAGGTCCCGA TCACTGGACT 
CCACAGCCTC AGGTGTCCTC CTCGCTGGAG TCGTCCACGT CCAGCCAGGA GCACCTCAAC 
TTCTCCGCCT CCTCCTCGTC CTCCAACAAA ACCCAGAACC AGAAGACCGG GCCGGGCCGG 
TGGAAGACTC CCAACGCCCC TCACTCCGTG CCGCCACATT CAGCCCAGCC GCCCTCCCGC 
TCCGACCTGC CCCCTCCTCC GCCTCCACCG CCAGCTACTT ACCCCGAATC CTACGACCCA 
TACGACCCCC AACCGGACCT GCCGCTCCCG CCGCCTCCCT CCGCCGTCAC TCCTGCGGTA 
GCTCAGCAGG CGGCCGACCG CAAGAAACGT GAGGAGCAGC AGCGCTGGTA TGAGAAGGAG 
AAAGCTCGGC TGGAGGAGGA GAGGGAGAGG AAGAGGAGAG AGCAGGAGAG GAAGCTGGGT 
CAGATCCGGG CCAACCCCGT CATGCCCGTC CAGCCTCACA ACAACGGAGG ATCCATGCCT 
CCGCCTCACC ACCAGCCGCC TCTGCCGTCT GCAGCCCCGC CTCAGCCCTA CCTTCCCCCC 
GCTCAGCCCC AGCCTCCACC ATCCAGACCG GAGAAGCTGA CCAGCCTGCC GCGGTCAGCC 
GTTCCTCCAC CCGCCAGCGC CTCCAACCCT GCAGGCATGG ACACGGTGAT CAGGGAGCTG 
TTGCCACAGC AGCAGCCGCG GACCATCGAG AGGCGAGACC TGCAGTACAT CACCATCAGC 
AAGGAGGAGC TCACCTCCAG CGACAGTCTG TCTCCAGACC CCTGGAAGCG AGACGCCCGC 
GAGAAGGCCG AGAAGCAGCA GCAGCTCCAC ATCGTCGACC TGCTGGACAA GGAGATCCAG 
GAGCTCCAGG CGAAGCCGGA GCGGACGGCG GAGGAGAGCG ACCGCCTCAG GAAGCTGATG 
CTGGAGTGGC AGTTCCAGAA ACGACTGCAG GAGTCCAAGC AGAGCGACGA AGACGAGGAG 
GAGGAGGACG ACGAGGACGT GGACACCATG ATGATCATGC AGAGGCTGGA GGCCGAGAAG 
AGAGCCAGGC AGCAGAATGC TGTCCCAGCT ATCTCTGTTC TAGACTTGTT GCAGGACGAG 
GAGCGGCGGC GTAAGCAGCA GCTGGAGGAG ATTCGTAAGC GGGAGGCGGA GGAGCGAGCA 
AAGCAGGAGG AGGAGAGGAG GTGGAGGGAG GAGGAGAGAG CCAAGCGAGA GGCCGAGGAG 
AAGAGGAGAC AGGAGGAGGA GTACTACACT CGCCTGGAGG CGGAGCGGCG CCGGCAGCAC 
GACGAGGCCG AGAGGAAGCT GCTGACGCCC GACGACCCGG CCGGTCTGTA TCGGCCCCCG 
CTGCCCCGGG ATTACCAAAC CCCGCCCCCC CAACAAACCT ATAAAACCCC CCCGCCCCCC 
CCACAGAGAA ACACCTCCTA CCTCAAAACG CAGGTCACCT CCCCCGACAC GCTCTACACC 
GCCAAGTTTG TCTCCTACGC GGGCGACGAG GAGGAAGACG AGGAGGCAGG GAGTGGCGGC 
GGCGGCGGCA GCAGCAGCCA ATCAGGTCAG GTGAAGCTGT CGGCGACCCG GAAGTCGTAC 
GGCGACCTCC CGGCCTCCGC CTCGCCCAAT CACAGCAGGC CTCAGCCGCC GCCCACTGCG 
CGCAAGCCCC GCCCTGTTTC TGACGGCATC TTCCTGTCTG GCTCGTTCCA GCCGCCATTC 
AACAACAACT CCAACAGTAC AGGTCCCCCC CTTCCTGCCA AACCCAGCTC CATCCCCCCG 
GGAGGCTTTA AAGGATTTAA CTCGTACACT GGAAACTCAG CAGGAGTTGT AGGATCAGGA 
GAAGTCTACA AGGACCCGCG AGACAAATGG ACGAAGAGTC AGGAGCAGGA GAACTCGAAC 
CCCAGCGGCC CTCCGGAGAA TCTGACCTTC AAGGAGCGGC AGCGTCTCTT CTCTCAGGGG 
AAGGAGGTTT CCAACAAGGT CAAAGCTTCG CGCAAACTCA TGGAGCTGGA GAACGAGCTC 
AACACCAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afdn ( XM_027284625.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_027140426.1 Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X5, mRNA.

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CloneID	OLa200707
Clone ID Related Accession (Same CDS sequence)	XM_027284625.1
Accession Version	XM_027284625.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	afadin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040021.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGCA GCAGGGAGGA GGAGAGGAGG AAACTGGCCG ACATCATCAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CGGCCCACAG AGGACCTGGA GTTCCATGGC 
GTGATGCGGT TCTACTTCCA GGACCGGGTC GCCGGTAACT TTGCCACCAA ATGCATCAGA 
GTGTCGAGCA CGGCGACAAC GCAGGACGTC ATCGAGACGT TAGCAGAGAA GTTCAGACCG 
GACATGAGGA TGCTCTCCTC GCCAAAATAC TCTTTGTACG AGGTCCACGT CAGTGGCGAG 
CGTCAGCTGG ATCTGGACGA GAAGCCTCTG GTGGTTCAGC TGAACTGGAA CAAAGACGAC 
AGAGAAGGAC GCTTCGTCCT GAAGAACGAG AACGACATCC TCCCCAAGAG TCAGAGTAAC 
GGCCCGGAGA AGGAGAAGGA CGGAGTGATC CAGAACTTCA AGAGGACTTT GTCCAAGAAG 
GAGAAGAAGA AGGAGAAGAA GAGGGAGAAG GAGTTCGCCC GGATCCCCGA CGGAGACGAG 
CAGGCGCTCA GCCGAGAGGA CGGGGAGAAC AGTCGTCTGG CGGCGGAGGT TTACAAGGAC 
ATGCCGGAGA ACAGCTTCAC CAGGACGATC TCCAACCCGG AGGTGGTGAT GAAGAGGAGA 
CGCCAGCAGA AGCTGGAGAA ACGCATGCAG GAGTTCATGA GCAGCGACGG GAGGCCCGAC 
TCAGGGGGCA CGTTGCGGAT ATACGCCGAC AGCCTGAAGC CGAACATCCC GTATAAAACC 
ATCCTGCTGT CCACGAGAGA CACGGCTGAC TTTGCGGTGG TGGAGGCGCT GGAGAAGTAC 
GGGCTGGAGA AGGAGAACCC CCGAGAGTAC TGCATCGCCC GGCAAGACGA TAAGTCCGGC 
AAGGAGGCGA TCCTGGACGA TACCGAGTGT CCACTGCAGA TCTTCAGGGA CTGGCCTGCA 
GACAGAGGAG CGCTAGTGTT CCAGCTGAAG AAGAGACCCC CGGACTACCA GGGCAGGAAG 
GGGAGGAAAG TGGACGACAA GGGCCTGCGA GGGAAGGACG GCAGCAGCTC CCTGCCGCCC 
GAGAAACTGC CTTACCTGGT GGAGCTGAGC CCAGGGCGAG GGAATCACTA TGCCTACTAC 
GCCTATCGGC ACCACGAAGA CGGGTCTGAC TCACGGGACA AACCAAAGCT GTACCGGCTC 
CAGCACAGCA TCACTGAGGT CGGCTCAGAC TGCACAGAGG ACGGAGCCAT TCAGCTGCTC 
GGTCCGGGGA TTCTGCCCCA CCACTGCAAC CTCATGCACA GTGAAGGCAT GGTGACGGTG 
ACGCCGCACG GCCCCGACGC CGACACCTTC GTCGACGGGC AACGGGTCAC TGAGACGACG 
GTGCTGCGCT CGGGCTCCAC CCTCCAGTTT GGCCAAGCGC ACGTCTTCAA GTTTGTGGAC 
CCGATGTTCG ACCAGGGAGG GAAGAGAGAG CCGGCCGCCA TGATGAGGAG TCGACACAAG 
TCCGGCAGCG TACCCGAAAC CACCTTCGAC CTTCACGGAG ACGTCCACAG CGGAGCCGCG 
CTGCCTACCA GCAAGAGCTC AGGAAAGCTG GAGATGGAGA GAGGAATGGT CAAACCGATG 
ATCAGAGGAG AGCAGCAGGA CGGCAGGTCT CAGGACATTT CAGCAGACAG AGCCGAGTTG 
ACTCTGCCAG CCGGCATCGA GTTCAGAGAC AACTCTGAAG ACACCTTCCT GTCGGCCATC 
ATCAACTACA CCAACAGCTC CACGGTTCAC TTCAAACTCT CGCCCACGTA CGTCCTGTAC 
ATGGCCTGCC GCTACATCCT GTCGCCCTCG TACCGGCCCG ACATGTCGCC GTCCGAGAGG 
ACGCACAAGG TCATCGCCAT CGTCAACAAG ATGGTGAGCA TGATGGAGGG AGTCATCCAG 
GAGATTCCTG AAGGGGATCA GAAGCAGAAG AACATCGCGG GAGCGCTGGC TTTCTGGATG 
GCCAACGCCT CCGAGCTGCT CAACTTCATC AAGCAGGACC GAGACCTGAA CCGCATCACG 
CTGGACGCCC AGGACATCCT CGCCCATCTG GTTCAGATGG CTTTCAAGTA CCTGGTCCAC 
TGTCTGCAGG CTGATCTGAA TAACTACATG CCGGCCTTCC TGGACGACCC CGAGGAACAC 
AACCCACAGA GACCCAAGAT CGAGGACGTC CTGCACACCC TCACGGGGGC GATGTCGTTA 
CTGCGGCGGT GTCGGGTCAA CGCCGCTCTC ACCATCCAGC TCTTCTCGCA GCTCTTCCAC 
TTCATCAACA TGTGGCTCTT CAACAAGCTG GTGACGGACA CCGAGTCCGG GCTGTGCTGT 
CACTACTGGG GGGCCATCCT GCGGCAGCAG CTCAGCCACA TCGAGGCCTG GGCGGAGAAA 
CAGGGGCTGG AGCTCGCCGC CGACTGCCAC CTCAGTCGAA TCGTGCAGGC CACCACCCTG 
CTGACCATGG ACAAATACTC CATGCAGGAT GTGCCGAACA TCCACAACAC CTGCTTCAAG 
CTCAACTCGC TGCAGCTCCA CGCCCTCATG AACAACTACC ACTGCGCTCC GGACGAGCCG 
TACATCCCGC CGGAGCTGAT AGACCACGTG GTGGCAGTGG CAGAGAACAC AGCAGATGAG 
CTGGCGAGGA GCGACGGCAG AGAGGTTCAG CTGGAGGAGG ATCCGGACCT CCAGCTGCCG 
TTCCTCCTTC CCGAGGACGG TTACTCCTGC GACGTGGTGC GCAGCCTCCC CAACGGCCTG 
CAGGACTTCC TGGAGCCGCT GCTGCAGAGA GGTTTCTGTC GGTTGGTACC TCACCCTCGC 
TCTCCCGGTA CCTGGACGGT CCATTTTGAA GGAGCTGACT GCGACAGCCA CTTCTCTGCT 
GCTGACAACT CAGAGATGCC AATGAGGAAA GAGCCGGAGG TTGTCACGGT AACCCTGAAG 
AAGCACAACG GCATGGGCCT CAGCATCGTG GCTGCCAAGG GTGCTGGTCA GGAGAAGCTG 
GGCATCTACA TCAAGTCTGT CGTCAAAGGA GGAGCAGCTG ATGTGGACGG GCGTCTGGCT 
GCAGGTGACC AGCTGTTGAG CGTGGACGGG CGGAGTCTGG TCGGCCTATC ACAAGAGAGG 
GCAGCGGAGC TGATGACGAG GACGGGCTCA GTCGTGACTC TGGAGGTCGC CAAGCAGGGA 
GCCATTTACC ACGGACTGGC CACGCTCCTC AACCAGCCGA GCCCCATGAT GCCACGAGCG 
TCAGACAGAG GCCGCGACAA GAACGGGAAA CTCCGACCAA AGAGCGAAGG CTTCGAGTTG 
TACAACAGCT CGGTGCAGAA CGGCTCTCCG GAGAGCCCGC AGGCCGGCTG GGACTCTTAC 
CCCGAACCAA AGAAGATGAG CGGCGAAGAT CGACTCCTGA AGAACCGAGC CGACCACCGC 
TCCAGCCCCA ACGTCGCCAA TCAGGGCCAG AGTCCTGCAG GCAAAGCAGT GTACCCCGGA 
GGACCTGGCA CCAAGATCAC CTCCGTCTCC ACCGGCAACC TGTGCGCAGA CGATGAGCCC 
TCCCCTCCAC GTCCAGAAGC TTACCCCATC CCCACCCAGA CCTACCCCAG GGAGTACTTC 
ACCATCCCGG CTTCCAAGAG CCAGGATCGG GTCGTTGGTC CTGGGCAGGG ACAGCACTGG 
CAGGGCATGG AGGACAGAGA ACGCCTCCCT GTGGTCGACA ACATCCACAA CAGCATGCAG 
CGGGTTAACC ACTCCCAGGA GGAGTTGTAC CCTCCACCCG GAGGCCTGAG GCCGGAGGAG 
CGCATGCAGC AGCACTACCA GCAGGACTAC CAGCACCGCG ACATGGACTA CCAGCACAGA 
GAGCTCGACT ACCAGAGAGG AGGTCCACCA GGTGTCGGAG GTCCCGATCA CTGGACTCCA 
CAGCCTCAGG TGTCCTCCTC GCTGGAGTCG TCCACGTCCA GCCAGGAGCA CCTCAACTTC 
TCCGCCTCCT CCTCGTCCTC CAACAAAACC CAGAACCAGA AGACCGGGCC GGGCCGGTGG 
AAGACTCCCA ACGCCCCTCA CTCCGTGCCG CCACATTCAG CCCAGCCGCC CTCCCGCTCC 
GACCTGCCCC CTCCTCCGCC TCCACCGCCA GCTACTTACC CCGAATCCTA CGACCCATAC 
GACCCCCAAC CGGACCTGCC GCTCCCGCCG CCTCCCTCCG CCGTCACTCC TGCGGTAGCT 
CAGCAGGCGG CCGACCGCAA GAAACGTGAG GAGCAGCAGC GCTGGTATGA GAAGGAGAAA 
GCTCGGCTGG AGGAGGAGAG GGAGAGGAAG AGGAGAGAGC AGGAGAGGAA GCTGGGTCAG 
ATCCGGGCCA ACCCCGTCAT GCCCGTCCAG CCTCACAACA ACGGAGGATC CATGCCTCCG 
CCTCACCACC AGCCGCCTCT GCCGTCTGCA GCCCCGCCTC AGCCCTACCT TCCCCCCGCT 
CAGCCCCAGC CTCCACCATC CAGACCGGAG AAGCTGACCA GCCTGCCGCG GTCAGCCGTT 
CCTCCACCCG CCAGCGCCTC CAACCCTGCA GGCATGGACA CGGTGATCAG GGAGCTGTTG 
CCACAGCAGC AGCCGCGGAC CATCGAGAGG CGAGACCTGC AGTACATCAC CATCAGCAAG 
GAGGAGCTCA CCTCCAGCGA CAGTCTGTCT CCAGACCCCT GGAAGCGAGA CGCCCGCGAG 
AAGGCCGAGA AGCAGCAGCA GCTCCACATC GTCGACCTGC TGGACAAGGA GATCCAGGAG 
CTCCAGGCGA AGCCGGAGCG GACGGCGGAG GAGAGCGACC GCCTCAGGAA GCTGATGCTG 
GAGTGGCAGT TCCAGAAACG ACTGCAGGAG TCCAAGCAGA GCGACGAAGA CGAGGAGGAG 
GAGGACGACG AGGACGTGGA CACCATGATG ATCATGCAGA GGCTGGAGGC CGAGAAGAGA 
GCCAGGCAGC AGAATGCTGT CCCAGCTATC TCTGTTCTAG ACTTGTTGCA GGACGAGGAG 
CGGCGGCGTA AGCAGCAGCT GGAGGAGATT CGTAAGCGGG AGGCGGAGGA GCGAGCAAAG 
CAGGAGGAGG AGAGGAGGTG GAGGGAGGAG GAGAGAGCCA AGCGAGAGGC CGAGGAGAAG 
AGGAGACAGG AGGAGGAGTA CTACACTCGC CTGGAGGCGG AGCGGCGCCG GCAGCACGAC 
GAGGCCGAGA GGAAGCTGCT GACGCCCGAC GACCCGGCCG GTCTGTATCG GCCCCCGCTG 
CCCCGGGATT ACCAAACCCC GCCCCCCCAA CAAACCTATA AAACCCCCCC GCCCCCCCCA 
CAGAGAAACA CCTCCTACCT CAAAACGCAG GTCACCTCCC CCGACACGCT CTACACCGCC 
AAGTTTGTCT CCTACGCGGG CGACGAGGAG GAAGACGAGG AGGCAGGGAG TGGCGGCGGC 
GGCGGCAGCA GCAGCCAATC AGGTCAGGTG AAGCTGTCGG CGACCCGGAA GTCGTACGGC 
GACCTCCCGG CCTCCGCCTC GCCCAATCAC AGCAGGCCTC AGCCGCCGCC CACTGCGCGC 
AAGCCCCGCC CTGTTTCTGA CGGCATCTTC CTGTCTGGCT CGTTCCAGCC GCCATTCAAC 
AACAACTCCA ACAGTACAGG TCCCCCCCTT CCTGCCAAAC CCAGCTCCAT CCCCCCGGGA 
GGCTTTAAAG GATTTAACTC GTACACTGGA AACTCAGCAG GAGTTGTAGG ATCAGGAGAA 
GTCTACAAGG ACCCGCGAGA CAAATGGACG AAGAGTCAGG AGCAGGAGAA CTCGAACCCC 
AGCGGCCCTC CGGAGAATCT GACCTTCAAG GAGCGGCAGC GTCTCTTCTC TCAGGGGAAG 
GAGGTTTCCA ACAAGGTCAA AGCTTCGCGC AAACTCATGG AGCTGGAGAA CGAGCTCAAC 
ACCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027140426.1
CDS	234..6122
Translation	MSGSREEERRKLADIINHWNANRLDLFEISRPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERQLDLDEKPLVVQLNWNKDDREGRFVLKNENDILPKSQSNGPEKEKDGVIQNFKRTLSKKEKKKEKKREKEFARIPDGDEQALSREDGENSRLAAEVYKDMPENSFTRTISNPEVVMKRRRQQKLEKRMQEFMSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTRDTADFAVVEALEKYGLEKENPREYCIARQDDKSGKEAILDDTECPLQIFRDWPADRGALVFQLKKRPPDYQGRKGRKVDDKGLRGKDGSSSLPPEKLPYLVELSPGRGNHYAYYAYRHHEDGSDSRDKPKLYRLQHSITEVGSDCTEDGAIQLLGPGILPHHCNLMHSEGMVTVTPHGPDADTFVDGQRVTETTVLRSGSTLQFGQAHVFKFVDPMFDQGGKREPAAMMRSRHKSGSVPETTFDLHGDVHSGAALPTSKSSGKLEMERGMVKPMIRGEQQDGRSQDISADRAELTLPAGIEFRDNSEDTFLSAIINYTNSSTVHFKLSPTYVLYMACRYILSPSYRPDMSPSERTHKVIAIVNKMVSMMEGVIQEIPEGDQKQKNIAGALAFWMANASELLNFIKQDRDLNRITLDAQDILAHLVQMAFKYLVHCLQADLNNYMPAFLDDPEEHNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVTDTESGLCCHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVPNIHNTCFKLNSLQLHALMNNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRSLPNGLQDFLEPLLQRGFCRLVPHPRSPGTWTVHFEGADCDSHFSAADNSEMPMRKEPEVVTVTLKKHNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRDKNGKLRPKSEGFELYNSSVQNGSPESPQAGWDSYPEPKKMSGEDRLLKNRADHRSSPNVANQGQSPAGKAVYPGGPGTKITSVSTGNLCADDEPSPPRPEAYPIPTQTYPREYFTIPASKSQDRVVGPGQGQHWQGMEDRERLPVVDNIHNSMQRVNHSQEELYPPPGGLRPEERMQQHYQQDYQHRDMDYQHRELDYQRGGPPGVGGPDHWTPQPQVSSSLESSTSSQEHLNFSASSSSSNKTQNQKTGPGRWKTPNAPHSVPPHSAQPPSRSDLPPPPPPPPATYPESYDPYDPQPDLPLPPPPSAVTPAVAQQAADRKKREEQQRWYEKEKARLEEERERKRREQERKLGQIRANPVMPVQPHNNGGSMPPPHHQPPLPSAAPPQPYLPPAQPQPPPSRPEKLTSLPRSAVPPPASASNPAGMDTVIRELLPQQQPRTIERRDLQYITISKEELTSSDSLSPDPWKRDAREKAEKQQQLHIVDLLDKEIQELQAKPERTAEESDRLRKLMLEWQFQKRLQESKQSDEDEEEEDDEDVDTMMIMQRLEAEKRARQQNAVPAISVLDLLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERAKREAEEKRRQEEEYYTRLEAERRRQHDEAERKLLTPDDPAGLYRPPLPRDYQTPPPQQTYKTPPPPPQRNTSYLKTQVTSPDTLYTAKFVSYAGDEEEDEEAGSGGGGGSSSQSGQVKLSATRKSYGDLPASASPNHSRPQPPPTARKPRPVSDGIFLSGSFQPPFNNNSNSTGPPLPAKPSSIPPGGFKGFNSYTGNSAGVVGSGEVYKDPRDKWTKSQEQENSNPSGPPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNTK

Target ORF information:

RefSeq Version	XM_027284625.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027284625.1

ORF Insert Sequence:

ATGTCGGGCA GCAGGGAGGA GGAGAGGAGG AAACTGGCCG ACATCATCAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CGGCCCACAG AGGACCTGGA GTTCCATGGC 
GTGATGCGGT TCTACTTCCA GGACCGGGTC GCCGGTAACT TTGCCACCAA ATGCATCAGA 
GTGTCGAGCA CGGCGACAAC GCAGGACGTC ATCGAGACGT TAGCAGAGAA GTTCAGACCG 
GACATGAGGA TGCTCTCCTC GCCAAAATAC TCTTTGTACG AGGTCCACGT CAGTGGCGAG 
CGTCAGCTGG ATCTGGACGA GAAGCCTCTG GTGGTTCAGC TGAACTGGAA CAAAGACGAC 
AGAGAAGGAC GCTTCGTCCT GAAGAACGAG AACGACATCC TCCCCAAGAG TCAGAGTAAC 
GGCCCGGAGA AGGAGAAGGA CGGAGTGATC CAGAACTTCA AGAGGACTTT GTCCAAGAAG 
GAGAAGAAGA AGGAGAAGAA GAGGGAGAAG GAGTTCGCCC GGATCCCCGA CGGAGACGAG 
CAGGCGCTCA GCCGAGAGGA CGGGGAGAAC AGTCGTCTGG CGGCGGAGGT TTACAAGGAC 
ATGCCGGAGA ACAGCTTCAC CAGGACGATC TCCAACCCGG AGGTGGTGAT GAAGAGGAGA 
CGCCAGCAGA AGCTGGAGAA ACGCATGCAG GAGTTCATGA GCAGCGACGG GAGGCCCGAC 
TCAGGGGGCA CGTTGCGGAT ATACGCCGAC AGCCTGAAGC CGAACATCCC GTATAAAACC 
ATCCTGCTGT CCACGAGAGA CACGGCTGAC TTTGCGGTGG TGGAGGCGCT GGAGAAGTAC 
GGGCTGGAGA AGGAGAACCC CCGAGAGTAC TGCATCGCCC GGCAAGACGA TAAGTCCGGC 
AAGGAGGCGA TCCTGGACGA TACCGAGTGT CCACTGCAGA TCTTCAGGGA CTGGCCTGCA 
GACAGAGGAG CGCTAGTGTT CCAGCTGAAG AAGAGACCCC CGGACTACCA GGGCAGGAAG 
GGGAGGAAAG TGGACGACAA GGGCCTGCGA GGGAAGGACG GCAGCAGCTC CCTGCCGCCC 
GAGAAACTGC CTTACCTGGT GGAGCTGAGC CCAGGGCGAG GGAATCACTA TGCCTACTAC 
GCCTATCGGC ACCACGAAGA CGGGTCTGAC TCACGGGACA AACCAAAGCT GTACCGGCTC 
CAGCACAGCA TCACTGAGGT CGGCTCAGAC TGCACAGAGG ACGGAGCCAT TCAGCTGCTC 
GGTCCGGGGA TTCTGCCCCA CCACTGCAAC CTCATGCACA GTGAAGGCAT GGTGACGGTG 
ACGCCGCACG GCCCCGACGC CGACACCTTC GTCGACGGGC AACGGGTCAC TGAGACGACG 
GTGCTGCGCT CGGGCTCCAC CCTCCAGTTT GGCCAAGCGC ACGTCTTCAA GTTTGTGGAC 
CCGATGTTCG ACCAGGGAGG GAAGAGAGAG CCGGCCGCCA TGATGAGGAG TCGACACAAG 
TCCGGCAGCG TACCCGAAAC CACCTTCGAC CTTCACGGAG ACGTCCACAG CGGAGCCGCG 
CTGCCTACCA GCAAGAGCTC AGGAAAGCTG GAGATGGAGA GAGGAATGGT CAAACCGATG 
ATCAGAGGAG AGCAGCAGGA CGGCAGGTCT CAGGACATTT CAGCAGACAG AGCCGAGTTG 
ACTCTGCCAG CCGGCATCGA GTTCAGAGAC AACTCTGAAG ACACCTTCCT GTCGGCCATC 
ATCAACTACA CCAACAGCTC CACGGTTCAC TTCAAACTCT CGCCCACGTA CGTCCTGTAC 
ATGGCCTGCC GCTACATCCT GTCGCCCTCG TACCGGCCCG ACATGTCGCC GTCCGAGAGG 
ACGCACAAGG TCATCGCCAT CGTCAACAAG ATGGTGAGCA TGATGGAGGG AGTCATCCAG 
GAGATTCCTG AAGGGGATCA GAAGCAGAAG AACATCGCGG GAGCGCTGGC TTTCTGGATG 
GCCAACGCCT CCGAGCTGCT CAACTTCATC AAGCAGGACC GAGACCTGAA CCGCATCACG 
CTGGACGCCC AGGACATCCT CGCCCATCTG GTTCAGATGG CTTTCAAGTA CCTGGTCCAC 
TGTCTGCAGG CTGATCTGAA TAACTACATG CCGGCCTTCC TGGACGACCC CGAGGAACAC 
AACCCACAGA GACCCAAGAT CGAGGACGTC CTGCACACCC TCACGGGGGC GATGTCGTTA 
CTGCGGCGGT GTCGGGTCAA CGCCGCTCTC ACCATCCAGC TCTTCTCGCA GCTCTTCCAC 
TTCATCAACA TGTGGCTCTT CAACAAGCTG GTGACGGACA CCGAGTCCGG GCTGTGCTGT 
CACTACTGGG GGGCCATCCT GCGGCAGCAG CTCAGCCACA TCGAGGCCTG GGCGGAGAAA 
CAGGGGCTGG AGCTCGCCGC CGACTGCCAC CTCAGTCGAA TCGTGCAGGC CACCACCCTG 
CTGACCATGG ACAAATACTC CATGCAGGAT GTGCCGAACA TCCACAACAC CTGCTTCAAG 
CTCAACTCGC TGCAGCTCCA CGCCCTCATG AACAACTACC ACTGCGCTCC GGACGAGCCG 
TACATCCCGC CGGAGCTGAT AGACCACGTG GTGGCAGTGG CAGAGAACAC AGCAGATGAG 
CTGGCGAGGA GCGACGGCAG AGAGGTTCAG CTGGAGGAGG ATCCGGACCT CCAGCTGCCG 
TTCCTCCTTC CCGAGGACGG TTACTCCTGC GACGTGGTGC GCAGCCTCCC CAACGGCCTG 
CAGGACTTCC TGGAGCCGCT GCTGCAGAGA GGTTTCTGTC GGTTGGTACC TCACCCTCGC 
TCTCCCGGTA CCTGGACGGT CCATTTTGAA GGAGCTGACT GCGACAGCCA CTTCTCTGCT 
GCTGACAACT CAGAGATGCC AATGAGGAAA GAGCCGGAGG TTGTCACGGT AACCCTGAAG 
AAGCACAACG GCATGGGCCT CAGCATCGTG GCTGCCAAGG GTGCTGGTCA GGAGAAGCTG 
GGCATCTACA TCAAGTCTGT CGTCAAAGGA GGAGCAGCTG ATGTGGACGG GCGTCTGGCT 
GCAGGTGACC AGCTGTTGAG CGTGGACGGG CGGAGTCTGG TCGGCCTATC ACAAGAGAGG 
GCAGCGGAGC TGATGACGAG GACGGGCTCA GTCGTGACTC TGGAGGTCGC CAAGCAGGGA 
GCCATTTACC ACGGACTGGC CACGCTCCTC AACCAGCCGA GCCCCATGAT GCCACGAGCG 
TCAGACAGAG GCCGCGACAA GAACGGGAAA CTCCGACCAA AGAGCGAAGG CTTCGAGTTG 
TACAACAGCT CGGTGCAGAA CGGCTCTCCG GAGAGCCCGC AGGCCGGCTG GGACTCTTAC 
CCCGAACCAA AGAAGATGAG CGGCGAAGAT CGACTCCTGA AGAACCGAGC CGACCACCGC 
TCCAGCCCCA ACGTCGCCAA TCAGGGCCAG AGTCCTGCAG GCAAAGCAGT GTACCCCGGA 
GGACCTGGCA CCAAGATCAC CTCCGTCTCC ACCGGCAACC TGTGCGCAGA CGATGAGCCC 
TCCCCTCCAC GTCCAGAAGC TTACCCCATC CCCACCCAGA CCTACCCCAG GGAGTACTTC 
ACCATCCCGG CTTCCAAGAG CCAGGATCGG GTCGTTGGTC CTGGGCAGGG ACAGCACTGG 
CAGGGCATGG AGGACAGAGA ACGCCTCCCT GTGGTCGACA ACATCCACAA CAGCATGCAG 
CGGGTTAACC ACTCCCAGGA GGAGTTGTAC CCTCCACCCG GAGGCCTGAG GCCGGAGGAG 
CGCATGCAGC AGCACTACCA GCAGGACTAC CAGCACCGCG ACATGGACTA CCAGCACAGA 
GAGCTCGACT ACCAGAGAGG AGGTCCACCA GGTGTCGGAG GTCCCGATCA CTGGACTCCA 
CAGCCTCAGG TGTCCTCCTC GCTGGAGTCG TCCACGTCCA GCCAGGAGCA CCTCAACTTC 
TCCGCCTCCT CCTCGTCCTC CAACAAAACC CAGAACCAGA AGACCGGGCC GGGCCGGTGG 
AAGACTCCCA ACGCCCCTCA CTCCGTGCCG CCACATTCAG CCCAGCCGCC CTCCCGCTCC 
GACCTGCCCC CTCCTCCGCC TCCACCGCCA GCTACTTACC CCGAATCCTA CGACCCATAC 
GACCCCCAAC CGGACCTGCC GCTCCCGCCG CCTCCCTCCG CCGTCACTCC TGCGGTAGCT 
CAGCAGGCGG CCGACCGCAA GAAACGTGAG GAGCAGCAGC GCTGGTATGA GAAGGAGAAA 
GCTCGGCTGG AGGAGGAGAG GGAGAGGAAG AGGAGAGAGC AGGAGAGGAA GCTGGGTCAG 
ATCCGGGCCA ACCCCGTCAT GCCCGTCCAG CCTCACAACA ACGGAGGATC CATGCCTCCG 
CCTCACCACC AGCCGCCTCT GCCGTCTGCA GCCCCGCCTC AGCCCTACCT TCCCCCCGCT 
CAGCCCCAGC CTCCACCATC CAGACCGGAG AAGCTGACCA GCCTGCCGCG GTCAGCCGTT 
CCTCCACCCG CCAGCGCCTC CAACCCTGCA GGCATGGACA CGGTGATCAG GGAGCTGTTG 
CCACAGCAGC AGCCGCGGAC CATCGAGAGG CGAGACCTGC AGTACATCAC CATCAGCAAG 
GAGGAGCTCA CCTCCAGCGA CAGTCTGTCT CCAGACCCCT GGAAGCGAGA CGCCCGCGAG 
AAGGCCGAGA AGCAGCAGCA GCTCCACATC GTCGACCTGC TGGACAAGGA GATCCAGGAG 
CTCCAGGCGA AGCCGGAGCG GACGGCGGAG GAGAGCGACC GCCTCAGGAA GCTGATGCTG 
GAGTGGCAGT TCCAGAAACG ACTGCAGGAG TCCAAGCAGA GCGACGAAGA CGAGGAGGAG 
GAGGACGACG AGGACGTGGA CACCATGATG ATCATGCAGA GGCTGGAGGC CGAGAAGAGA 
GCCAGGCAGC AGAATGCTGT CCCAGCTATC TCTGTTCTAG ACTTGTTGCA GGACGAGGAG 
CGGCGGCGTA AGCAGCAGCT GGAGGAGATT CGTAAGCGGG AGGCGGAGGA GCGAGCAAAG 
CAGGAGGAGG AGAGGAGGTG GAGGGAGGAG GAGAGAGCCA AGCGAGAGGC CGAGGAGAAG 
AGGAGACAGG AGGAGGAGTA CTACACTCGC CTGGAGGCGG AGCGGCGCCG GCAGCACGAC 
GAGGCCGAGA GGAAGCTGCT GACGCCCGAC GACCCGGCCG GTCTGTATCG GCCCCCGCTG 
CCCCGGGATT ACCAAACCCC GCCCCCCCAA CAAACCTATA AAACCCCCCC GCCCCCCCCA 
CAGAGAAACA CCTCCTACCT CAAAACGCAG GTCACCTCCC CCGACACGCT CTACACCGCC 
AAGTTTGTCT CCTACGCGGG CGACGAGGAG GAAGACGAGG AGGCAGGGAG TGGCGGCGGC 
GGCGGCAGCA GCAGCCAATC AGGTCAGGTG AAGCTGTCGG CGACCCGGAA GTCGTACGGC 
GACCTCCCGG CCTCCGCCTC GCCCAATCAC AGCAGGCCTC AGCCGCCGCC CACTGCGCGC 
AAGCCCCGCC CTGTTTCTGA CGGCATCTTC CTGTCTGGCT CGTTCCAGCC GCCATTCAAC 
AACAACTCCA ACAGTACAGG TCCCCCCCTT CCTGCCAAAC CCAGCTCCAT CCCCCCGGGA 
GGCTTTAAAG GATTTAACTC GTACACTGGA AACTCAGCAG GAGTTGTAGG ATCAGGAGAA 
GTCTACAAGG ACCCGCGAGA CAAATGGACG AAGAGTCAGG AGCAGGAGAA CTCGAACCCC 
AGCGGCCCTC CGGAGAATCT GACCTTCAAG GAGCGGCAGC GTCTCTTCTC TCAGGGGAAG 
GAGGTTTCCA ACAAGGTCAA AGCTTCGCGC AAACTCATGG AGCTGGAGAA CGAGCTCAAC 
ACCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afdn ( XM_027284626.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_027140427.1 Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X6, mRNA.

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CloneID	OLa200708
Clone ID Related Accession (Same CDS sequence)	XM_027284626.1
Accession Version	XM_027284626.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	afadin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040021.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGCA GCAGGGAGGA GGAGAGGAGG AAACTGGCCG ACATCATCAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CGGCCCACAG AGGACCTGGA GTTCCATGGC 
GTGATGCGGT TCTACTTCCA GGACCGGGTC GCCGGTAACT TTGCCACCAA ATGCATCAGA 
GTGTCGAGCA CGGCGACAAC GCAGGACGTC ATCGAGACGT TAGCAGAGAA GTTCAGACCG 
GACATGAGGA TGCTCTCCTC GCCAAAATAC TCTTTGTACG AGGTCCACGT CAGTGGCGAA 
GAGCGTCAGC TGGATCTGGA CGAGAAGCCT CTGGTGGTTC AGCTGAACTG GAACAAAGAC 
GACAGAGAAG GACGCTTCGT CCTGAAGAAC GAGAACGACA TCCTCCCCAA GAAGAGTCAG 
AGTAACGGCC CGGAGAAGGA GAAGGACGGA GTGATCCAGA ACTTCAAGAG GACTTTGTCC 
AAGAAGGAGA AGAAGAAGGA GAAGAAGAGG GAGAAGGAGT TCGCCCGGAT CCCCGACGGA 
GACGAGCAGG CGCTCAGCCG AGAGGACGGG GAGAACAGTC GTCTGGCGGC GGAGGTTTAC 
AAGGACATGC CGGAGAACAG CTTCACCAGG ACGATCTCCA ACCCGGAGGT GGTGATGAAG 
AGGAGACGCC AGCAGAAGCT GGAGAAACGC ATGCAGGAGT TCATGAGCAG CGACGGGAGG 
CCCGACTCAG GGGGCACGTT GCGGATATAC GCCGACAGCC TGAAGCCGAA CATCCCGTAT 
AAAACCATCC TGCTGTCCAC GAGAGACACG GCTGACTTTG CGGTGGTGGA GGCGCTGGAG 
AAGTACGGGC TGGAGAAGGA GAACCCCCGA GAGTACTGCA TCGCCCGGCA AGACGATAAG 
TCCGGCAAGG AGGCGATCCT GGACGATACC GAGTGTCCAC TGCAGATCTT CAGGGACTGG 
CCTGCAGACA GAGGAGCGCT AGTGTTCCAG CTGAAGAAGA GACCCCCGGA CTACCAGGGC 
AGGAAGGGGA GGAAAGTGGA CGACAAGGGC CTGCGAGGGA AGGACGGCAG CAGCTCCCTG 
CCGCCCGAGA AACTGCCTTA CCTGGTGGAG CTGAGCCCAG GGCGAGGGAA TCACTATGCC 
TACTACGCCT ATCGGCACCA CGAAGACGGG TCTGACTCAC GGGACAAACC AAAGCTGTAC 
CGGCTCCAGC ACAGCATCAC TGAGGTCGGC TCAGACTGCA CAGAGGACGG AGCCATTCAG 
CTGCTCGGTC CGGGGATTCT GCCCCACCAC TGCAACCTCA TGCACAGTGA AGGCATGGTG 
ACGGTGACGC CGCACGGCCC CGACGCCGAC ACCTTCGTCG ACGGGCAACG GGTCACTGAG 
ACGACGGTGC TGCGCTCGGG CTCCACCCTC CAGTTTGGCC AAGCGCACGT CTTCAAGTTT 
GTGGACCCGA TGTTCGACCA GGGAGGGAAG AGAGAGCCGG CCGCCATGAT GAGGAGTCGA 
CACAAGTCCG GCAGCGTACC CGAAACCACC TTCGACCTTC ACGGAGACGT CCACAGCGGA 
GCCGCGCTGC CTACCAGCAA GAGCTCAGGA AAGCTGGAGA TGGAGAGAGG AATGGTCAAA 
CCGATGATCA GAGGAGAGCA GCAGGACGGC AGGTCTCAGG ACATTTCAGC AGACAGAGCC 
GAGTTGACTC TGCCAGCCGG CATCGAGTTC AGAGACAACT CTGAAGACAC CTTCCTGTCG 
GCCATCATCA ACTACACCAA CAGCTCCACG GTTCACTTCA AACTCTCGCC CACGTACGTC 
CTGTACATGG CCTGCCGCTA CATCCTGTCG CCCTCGTACC GGCCCGACAT GTCGCCGTCC 
GAGAGGACGC ACAAGGTCAT CGCCATCGTC AACAAGATGG TGAGCATGAT GGAGGGAGTC 
ATCCAGAAGC AGAAGAACAT CGCGGGAGCG CTGGCTTTCT GGATGGCCAA CGCCTCCGAG 
CTGCTCAACT TCATCAAGCA GGACCGAGAC CTGAACCGCA TCACGCTGGA CGCCCAGGAC 
ATCCTCGCCC ATCTGGTTCA GATGGCTTTC AAGTACCTGG TCCACTGTCT GCAGGCTGAT 
CTGAATAACT ACATGCCGGC CTTCCTGGAC GACCCCGAGG AACACAACCC ACAGAGACCC 
AAGATCGAGG ACGTCCTGCA CACCCTCACG GGGGCGATGT CGTTACTGCG GCGGTGTCGG 
GTCAACGCCG CTCTCACCAT CCAGCTCTTC TCGCAGCTCT TCCACTTCAT CAACATGTGG 
CTCTTCAACA AGCTGGTGAC GGACACCGAG TCCGGGCTGT GCTGTCACTA CTGGGGGGCC 
ATCCTGCGGC AGCAGCTCAG CCACATCGAG GCCTGGGCGG AGAAACAGGG GCTGGAGCTC 
GCCGCCGACT GCCACCTCAG TCGAATCGTG CAGGCCACCA CCCTGCTGAC CATGGACAAA 
TACTCCATGC AGGATGTGCC GAACATCCAC AACACCTGCT TCAAGCTCAA CTCGCTGCAG 
CTCCACGCCC TCATGAACAA CTACCACTGC GCTCCGGACG AGCCGTACAT CCCGCCGGAG 
CTGATAGACC ACGTGGTGGC AGTGGCAGAG AACACAGCAG ATGAGCTGGC GAGGAGCGAC 
GGCAGAGAGG TTCAGCTGGA GGAGGATCCG GACCTCCAGC TGCCGTTCCT CCTTCCCGAG 
GACGGTTACT CCTGCGACGT GGTGCGCAGC CTCCCCAACG GCCTGCAGGA CTTCCTGGAG 
CCGCTGCTGC AGAGAGGTTT CTGTCGGTTG GTACCTCACC CTCGCTCTCC CGGTACCTGG 
ACGGTCCATT TTGAAGGAGC TGACTGCGAC AGCCACTTCT CTGCTGCTGA CAACTCAGAG 
ATGCCAATGA GGAAAGAGCC GGAGGTTGTC ACGGTAACCC TGAAGAAGCA CAACGGCATG 
GGCCTCAGCA TCGTGGCTGC CAAGGGTGCT GGTCAGGAGA AGCTGGGCAT CTACATCAAG 
TCTGTCGTCA AAGGAGGAGC AGCTGATGTG GACGGGCGTC TGGCTGCAGG TGACCAGCTG 
TTGAGCGTGG ACGGGCGGAG TCTGGTCGGC CTATCACAAG AGAGGGCAGC GGAGCTGATG 
ACGAGGACGG GCTCAGTCGT GACTCTGGAG GTCGCCAAGC AGGGAGCCAT TTACCACGGA 
CTGGCCACGC TCCTCAACCA GCCGAGCCCC ATGATGCCAC GAGCGTCAGA CAGAGGCCGC 
GACAAGAACG GGAAACTCCG ACCAAAGAGC GAAGGCTTCG AGTTGTACAA CAGCTCGGTG 
CAGAACGGCT CTCCGGAGAG CCCGCAGGCC GGCTGGGACT CTTACCCCGA ACCAAAGAAG 
ATGAGCGGCG AAGATCGACT CCTGAAGAAC CGAGCCGACC ACCGCTCCAG CCCCAACGTC 
GCCAATCAGG GCCAGAGTCC TGCAGGCAAA GCAGTGTACC CCGGAGGACC TGGCACCAAG 
ATCACCTCCG TCTCCACCGG CAACCTGTGC GCAGACGATG AGCCCTCCCC TCCACGTCCA 
GAAGCTTACC CCATCCCCAC CCAGACCTAC CCCAGGGAGT ACTTCACCAT CCCGGCTTCC 
AAGAGCCAGG ATCGGGTCGT TGGTCCTGGG CAGGGACAGC ACTGGCAGGG CATGGAGGAC 
AGAGAACGCC TCCCTGTGGT CGACAACATC CACAACAGCA TGCAGCGGGT TAACCACTCC 
CAGGAGGAGT TGTACCCTCC ACCCGGAGGC CTGAGGCCGG AGGAGCGCAT GCAGCAGCAC 
TACCAGCAGG ACTACCAGCA CCGCGACATG GACTACCAGC ACAGAGAGCT CGACTACCAG 
AGAGGAGGTC CACCAGGTGT CGGAGGTCCC GATCACTGGA CTCCACAGCC TCAGGTGTCC 
TCCTCGCTGG AGTCGTCCAC GTCCAGCCAG GAGCACCTCA ACTTCTCCGC CTCCTCCTCG 
TCCTCCAACA AAACCCAGAA CCAGAAGACC GGGCCGGGCC GGTGGAAGAC TCCCAACGCC 
CCTCACTCCG TGCCGCCACA TTCAGCCCAG CCGCCCTCCC GCTCCGACCT GCCCCCTCCT 
CCGCCTCCAC CGCCAGCTAC TTACCCCGAA TCCTACGACC CATACGACCC CCAACCGGAC 
CTGCCGCTCC CGCCGCCTCC CTCCGCCGTC ACTCCTGCGG TAGCTCAGCA GGCGGCCGAC 
CGCAAGAAAC GTGAGGAGCA GCAGCGCTGG TATGAGAAGG AGAAAGCTCG GCTGGAGGAG 
GAGAGGGAGA GGAAGAGGAG AGAGCAGGAG AGGAAGCTGG GTCAGATCCG GGCCAACCCC 
GTCATGCCCG TCCAGCCTCA CAACAACGGA GGATCCATGC CTCCGCCTCA CCACCAGCCG 
CCTCTGCCGT CTGCAGCCCC GCCTCAGCCC TACCTTCCCC CCGCTCAGCC CCAGCCTCCA 
CCATCCAGAC CGGAGAAGCT GACCAGCCTG CCGCGGTCAG CCGTTCCTCC ACCCGCCAGC 
GCCTCCAACC CTGCAGGCAT GGACACGGTG ATCAGGGAGC TGTTGCCACA GCAGCAGCCG 
CGGACCATCG AGAGGCGAGA CCTGCAGTAC ATCACCATCA GCAAGGAGGA GCTCACCTCC 
AGCGACAGTC TGTCTCCAGA CCCCTGGAAG CGAGACGCCC GCGAGAAGGC CGAGAAGCAG 
CAGCAGCTCC ACATCGTCGA CCTGCTGGAC AAGGAGATCC AGGAGCTCCA GGCGAAGCCG 
GAGCGGACGG CGGAGGAGAG CGACCGCCTC AGGAAGCTGA TGCTGGAGTG GCAGTTCCAG 
AAACGACTGC AGGAGTCCAA GCAGAGCGAC GAAGACGAGG AGGAGGAGGA CGACGAGGAC 
GTGGACACCA TGATGATCAT GCAGAGGCTG GAGGCCGAGA AGAGAGCCAG GCAGCAGAAT 
GCTGTCCCAG CTATCTCTGT TCTAGACTTG TTGCAGGACG AGGAGCGGCG GCGTAAGCAG 
CAGCTGGAGG AGATTCGTAA GCGGGAGGCG GAGGAGCGAG CAAAGCAGGA GGAGGAGAGG 
AGGTGGAGGG AGGAGGAGAG AGCCAAGCGA GAGGCCGAGG AGAAGAGGAG ACAGGAGGAG 
GAGTACTACA CTCGCCTGGA GGCGGAGCGG CGCCGGCAGC ACGACGAGGC CGAGAGGAAG 
CTGCTGACGC CCGACGACCC GGCCGGTCTG TATCGGCCCC CGCTGCCCCG GGATTACCAA 
ACCCCGCCCC CCCAACAAAC CTATAAAACC CCCCCGCCCC CCCCACAGAG AAACACCTCC 
TACCTCAAAA CGCAGGTCAC CTCCCCCGAC ACGCTCTACA CCGCCAAGTT TGTCTCCTAC 
GCGGGCGACG AGGAGGAAGA CGAGGAGGCA GGGAGTGGCG GCGGCGGCGG CAGCAGCAGC 
CAATCAGGTC AGGTGAAGCT GTCGGCGACC CGGAAGTCGT ACGGCGACCT CCCGGCCTCC 
GCCTCGCCCA ATCACAGCAG GCCTCAGCCG CCGCCCACTG CGCGCAAGCC CCGCCCTGTT 
TCTGACGGCA TCTTCCTGTC TGGCTCGTTC CAGCCGCCAT TCAACAACAA CTCCAACAGT 
ACAGGTCCCC CCCTTCCTGC CAAACCCAGC TCCATCCCCC CGGGAGGCTT TAAAGGATTT 
AACTCGTACA CTGGAAACTC AGCAGGAGTT GTAGGATCAG GAGAAGTCTA CAAGGACCCG 
CGAGACAAAT GGACGAAGAG TCAGGAGCAG GAGAACTCGA ACCCCAGCGG CCCTCCGGAG 
AATCTGACCT TCAAGGAGCG GCAGCGTCTC TTCTCTCAGG GGAAGGAGGT TTCCAACAAG 
GTCAAAGCTT CGCGCAAACT CATGGAGCTG GAGAACGAGC TCAACACCAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027140427.1
CDS	233..6106
Translation	MSGSREEERRKLADIINHWNANRLDLFEISRPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERQLDLDEKPLVVQLNWNKDDREGRFVLKNENDILPKKSQSNGPEKEKDGVIQNFKRTLSKKEKKKEKKREKEFARIPDGDEQALSREDGENSRLAAEVYKDMPENSFTRTISNPEVVMKRRRQQKLEKRMQEFMSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTRDTADFAVVEALEKYGLEKENPREYCIARQDDKSGKEAILDDTECPLQIFRDWPADRGALVFQLKKRPPDYQGRKGRKVDDKGLRGKDGSSSLPPEKLPYLVELSPGRGNHYAYYAYRHHEDGSDSRDKPKLYRLQHSITEVGSDCTEDGAIQLLGPGILPHHCNLMHSEGMVTVTPHGPDADTFVDGQRVTETTVLRSGSTLQFGQAHVFKFVDPMFDQGGKREPAAMMRSRHKSGSVPETTFDLHGDVHSGAALPTSKSSGKLEMERGMVKPMIRGEQQDGRSQDISADRAELTLPAGIEFRDNSEDTFLSAIINYTNSSTVHFKLSPTYVLYMACRYILSPSYRPDMSPSERTHKVIAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLNRITLDAQDILAHLVQMAFKYLVHCLQADLNNYMPAFLDDPEEHNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVTDTESGLCCHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVPNIHNTCFKLNSLQLHALMNNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRSLPNGLQDFLEPLLQRGFCRLVPHPRSPGTWTVHFEGADCDSHFSAADNSEMPMRKEPEVVTVTLKKHNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRDKNGKLRPKSEGFELYNSSVQNGSPESPQAGWDSYPEPKKMSGEDRLLKNRADHRSSPNVANQGQSPAGKAVYPGGPGTKITSVSTGNLCADDEPSPPRPEAYPIPTQTYPREYFTIPASKSQDRVVGPGQGQHWQGMEDRERLPVVDNIHNSMQRVNHSQEELYPPPGGLRPEERMQQHYQQDYQHRDMDYQHRELDYQRGGPPGVGGPDHWTPQPQVSSSLESSTSSQEHLNFSASSSSSNKTQNQKTGPGRWKTPNAPHSVPPHSAQPPSRSDLPPPPPPPPATYPESYDPYDPQPDLPLPPPPSAVTPAVAQQAADRKKREEQQRWYEKEKARLEEERERKRREQERKLGQIRANPVMPVQPHNNGGSMPPPHHQPPLPSAAPPQPYLPPAQPQPPPSRPEKLTSLPRSAVPPPASASNPAGMDTVIRELLPQQQPRTIERRDLQYITISKEELTSSDSLSPDPWKRDAREKAEKQQQLHIVDLLDKEIQELQAKPERTAEESDRLRKLMLEWQFQKRLQESKQSDEDEEEEDDEDVDTMMIMQRLEAEKRARQQNAVPAISVLDLLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERAKREAEEKRRQEEEYYTRLEAERRRQHDEAERKLLTPDDPAGLYRPPLPRDYQTPPPQQTYKTPPPPPQRNTSYLKTQVTSPDTLYTAKFVSYAGDEEEDEEAGSGGGGGSSSQSGQVKLSATRKSYGDLPASASPNHSRPQPPPTARKPRPVSDGIFLSGSFQPPFNNNSNSTGPPLPAKPSSIPPGGFKGFNSYTGNSAGVVGSGEVYKDPRDKWTKSQEQENSNPSGPPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNTK

Target ORF information:

RefSeq Version	XM_027284626.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027284626.1

ORF Insert Sequence:

ATGTCGGGCA GCAGGGAGGA GGAGAGGAGG AAACTGGCCG ACATCATCAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CGGCCCACAG AGGACCTGGA GTTCCATGGC 
GTGATGCGGT TCTACTTCCA GGACCGGGTC GCCGGTAACT TTGCCACCAA ATGCATCAGA 
GTGTCGAGCA CGGCGACAAC GCAGGACGTC ATCGAGACGT TAGCAGAGAA GTTCAGACCG 
GACATGAGGA TGCTCTCCTC GCCAAAATAC TCTTTGTACG AGGTCCACGT CAGTGGCGAA 
GAGCGTCAGC TGGATCTGGA CGAGAAGCCT CTGGTGGTTC AGCTGAACTG GAACAAAGAC 
GACAGAGAAG GACGCTTCGT CCTGAAGAAC GAGAACGACA TCCTCCCCAA GAAGAGTCAG 
AGTAACGGCC CGGAGAAGGA GAAGGACGGA GTGATCCAGA ACTTCAAGAG GACTTTGTCC 
AAGAAGGAGA AGAAGAAGGA GAAGAAGAGG GAGAAGGAGT TCGCCCGGAT CCCCGACGGA 
GACGAGCAGG CGCTCAGCCG AGAGGACGGG GAGAACAGTC GTCTGGCGGC GGAGGTTTAC 
AAGGACATGC CGGAGAACAG CTTCACCAGG ACGATCTCCA ACCCGGAGGT GGTGATGAAG 
AGGAGACGCC AGCAGAAGCT GGAGAAACGC ATGCAGGAGT TCATGAGCAG CGACGGGAGG 
CCCGACTCAG GGGGCACGTT GCGGATATAC GCCGACAGCC TGAAGCCGAA CATCCCGTAT 
AAAACCATCC TGCTGTCCAC GAGAGACACG GCTGACTTTG CGGTGGTGGA GGCGCTGGAG 
AAGTACGGGC TGGAGAAGGA GAACCCCCGA GAGTACTGCA TCGCCCGGCA AGACGATAAG 
TCCGGCAAGG AGGCGATCCT GGACGATACC GAGTGTCCAC TGCAGATCTT CAGGGACTGG 
CCTGCAGACA GAGGAGCGCT AGTGTTCCAG CTGAAGAAGA GACCCCCGGA CTACCAGGGC 
AGGAAGGGGA GGAAAGTGGA CGACAAGGGC CTGCGAGGGA AGGACGGCAG CAGCTCCCTG 
CCGCCCGAGA AACTGCCTTA CCTGGTGGAG CTGAGCCCAG GGCGAGGGAA TCACTATGCC 
TACTACGCCT ATCGGCACCA CGAAGACGGG TCTGACTCAC GGGACAAACC AAAGCTGTAC 
CGGCTCCAGC ACAGCATCAC TGAGGTCGGC TCAGACTGCA CAGAGGACGG AGCCATTCAG 
CTGCTCGGTC CGGGGATTCT GCCCCACCAC TGCAACCTCA TGCACAGTGA AGGCATGGTG 
ACGGTGACGC CGCACGGCCC CGACGCCGAC ACCTTCGTCG ACGGGCAACG GGTCACTGAG 
ACGACGGTGC TGCGCTCGGG CTCCACCCTC CAGTTTGGCC AAGCGCACGT CTTCAAGTTT 
GTGGACCCGA TGTTCGACCA GGGAGGGAAG AGAGAGCCGG CCGCCATGAT GAGGAGTCGA 
CACAAGTCCG GCAGCGTACC CGAAACCACC TTCGACCTTC ACGGAGACGT CCACAGCGGA 
GCCGCGCTGC CTACCAGCAA GAGCTCAGGA AAGCTGGAGA TGGAGAGAGG AATGGTCAAA 
CCGATGATCA GAGGAGAGCA GCAGGACGGC AGGTCTCAGG ACATTTCAGC AGACAGAGCC 
GAGTTGACTC TGCCAGCCGG CATCGAGTTC AGAGACAACT CTGAAGACAC CTTCCTGTCG 
GCCATCATCA ACTACACCAA CAGCTCCACG GTTCACTTCA AACTCTCGCC CACGTACGTC 
CTGTACATGG CCTGCCGCTA CATCCTGTCG CCCTCGTACC GGCCCGACAT GTCGCCGTCC 
GAGAGGACGC ACAAGGTCAT CGCCATCGTC AACAAGATGG TGAGCATGAT GGAGGGAGTC 
ATCCAGAAGC AGAAGAACAT CGCGGGAGCG CTGGCTTTCT GGATGGCCAA CGCCTCCGAG 
CTGCTCAACT TCATCAAGCA GGACCGAGAC CTGAACCGCA TCACGCTGGA CGCCCAGGAC 
ATCCTCGCCC ATCTGGTTCA GATGGCTTTC AAGTACCTGG TCCACTGTCT GCAGGCTGAT 
CTGAATAACT ACATGCCGGC CTTCCTGGAC GACCCCGAGG AACACAACCC ACAGAGACCC 
AAGATCGAGG ACGTCCTGCA CACCCTCACG GGGGCGATGT CGTTACTGCG GCGGTGTCGG 
GTCAACGCCG CTCTCACCAT CCAGCTCTTC TCGCAGCTCT TCCACTTCAT CAACATGTGG 
CTCTTCAACA AGCTGGTGAC GGACACCGAG TCCGGGCTGT GCTGTCACTA CTGGGGGGCC 
ATCCTGCGGC AGCAGCTCAG CCACATCGAG GCCTGGGCGG AGAAACAGGG GCTGGAGCTC 
GCCGCCGACT GCCACCTCAG TCGAATCGTG CAGGCCACCA CCCTGCTGAC CATGGACAAA 
TACTCCATGC AGGATGTGCC GAACATCCAC AACACCTGCT TCAAGCTCAA CTCGCTGCAG 
CTCCACGCCC TCATGAACAA CTACCACTGC GCTCCGGACG AGCCGTACAT CCCGCCGGAG 
CTGATAGACC ACGTGGTGGC AGTGGCAGAG AACACAGCAG ATGAGCTGGC GAGGAGCGAC 
GGCAGAGAGG TTCAGCTGGA GGAGGATCCG GACCTCCAGC TGCCGTTCCT CCTTCCCGAG 
GACGGTTACT CCTGCGACGT GGTGCGCAGC CTCCCCAACG GCCTGCAGGA CTTCCTGGAG 
CCGCTGCTGC AGAGAGGTTT CTGTCGGTTG GTACCTCACC CTCGCTCTCC CGGTACCTGG 
ACGGTCCATT TTGAAGGAGC TGACTGCGAC AGCCACTTCT CTGCTGCTGA CAACTCAGAG 
ATGCCAATGA GGAAAGAGCC GGAGGTTGTC ACGGTAACCC TGAAGAAGCA CAACGGCATG 
GGCCTCAGCA TCGTGGCTGC CAAGGGTGCT GGTCAGGAGA AGCTGGGCAT CTACATCAAG 
TCTGTCGTCA AAGGAGGAGC AGCTGATGTG GACGGGCGTC TGGCTGCAGG TGACCAGCTG 
TTGAGCGTGG ACGGGCGGAG TCTGGTCGGC CTATCACAAG AGAGGGCAGC GGAGCTGATG 
ACGAGGACGG GCTCAGTCGT GACTCTGGAG GTCGCCAAGC AGGGAGCCAT TTACCACGGA 
CTGGCCACGC TCCTCAACCA GCCGAGCCCC ATGATGCCAC GAGCGTCAGA CAGAGGCCGC 
GACAAGAACG GGAAACTCCG ACCAAAGAGC GAAGGCTTCG AGTTGTACAA CAGCTCGGTG 
CAGAACGGCT CTCCGGAGAG CCCGCAGGCC GGCTGGGACT CTTACCCCGA ACCAAAGAAG 
ATGAGCGGCG AAGATCGACT CCTGAAGAAC CGAGCCGACC ACCGCTCCAG CCCCAACGTC 
GCCAATCAGG GCCAGAGTCC TGCAGGCAAA GCAGTGTACC CCGGAGGACC TGGCACCAAG 
ATCACCTCCG TCTCCACCGG CAACCTGTGC GCAGACGATG AGCCCTCCCC TCCACGTCCA 
GAAGCTTACC CCATCCCCAC CCAGACCTAC CCCAGGGAGT ACTTCACCAT CCCGGCTTCC 
AAGAGCCAGG ATCGGGTCGT TGGTCCTGGG CAGGGACAGC ACTGGCAGGG CATGGAGGAC 
AGAGAACGCC TCCCTGTGGT CGACAACATC CACAACAGCA TGCAGCGGGT TAACCACTCC 
CAGGAGGAGT TGTACCCTCC ACCCGGAGGC CTGAGGCCGG AGGAGCGCAT GCAGCAGCAC 
TACCAGCAGG ACTACCAGCA CCGCGACATG GACTACCAGC ACAGAGAGCT CGACTACCAG 
AGAGGAGGTC CACCAGGTGT CGGAGGTCCC GATCACTGGA CTCCACAGCC TCAGGTGTCC 
TCCTCGCTGG AGTCGTCCAC GTCCAGCCAG GAGCACCTCA ACTTCTCCGC CTCCTCCTCG 
TCCTCCAACA AAACCCAGAA CCAGAAGACC GGGCCGGGCC GGTGGAAGAC TCCCAACGCC 
CCTCACTCCG TGCCGCCACA TTCAGCCCAG CCGCCCTCCC GCTCCGACCT GCCCCCTCCT 
CCGCCTCCAC CGCCAGCTAC TTACCCCGAA TCCTACGACC CATACGACCC CCAACCGGAC 
CTGCCGCTCC CGCCGCCTCC CTCCGCCGTC ACTCCTGCGG TAGCTCAGCA GGCGGCCGAC 
CGCAAGAAAC GTGAGGAGCA GCAGCGCTGG TATGAGAAGG AGAAAGCTCG GCTGGAGGAG 
GAGAGGGAGA GGAAGAGGAG AGAGCAGGAG AGGAAGCTGG GTCAGATCCG GGCCAACCCC 
GTCATGCCCG TCCAGCCTCA CAACAACGGA GGATCCATGC CTCCGCCTCA CCACCAGCCG 
CCTCTGCCGT CTGCAGCCCC GCCTCAGCCC TACCTTCCCC CCGCTCAGCC CCAGCCTCCA 
CCATCCAGAC CGGAGAAGCT GACCAGCCTG CCGCGGTCAG CCGTTCCTCC ACCCGCCAGC 
GCCTCCAACC CTGCAGGCAT GGACACGGTG ATCAGGGAGC TGTTGCCACA GCAGCAGCCG 
CGGACCATCG AGAGGCGAGA CCTGCAGTAC ATCACCATCA GCAAGGAGGA GCTCACCTCC 
AGCGACAGTC TGTCTCCAGA CCCCTGGAAG CGAGACGCCC GCGAGAAGGC CGAGAAGCAG 
CAGCAGCTCC ACATCGTCGA CCTGCTGGAC AAGGAGATCC AGGAGCTCCA GGCGAAGCCG 
GAGCGGACGG CGGAGGAGAG CGACCGCCTC AGGAAGCTGA TGCTGGAGTG GCAGTTCCAG 
AAACGACTGC AGGAGTCCAA GCAGAGCGAC GAAGACGAGG AGGAGGAGGA CGACGAGGAC 
GTGGACACCA TGATGATCAT GCAGAGGCTG GAGGCCGAGA AGAGAGCCAG GCAGCAGAAT 
GCTGTCCCAG CTATCTCTGT TCTAGACTTG TTGCAGGACG AGGAGCGGCG GCGTAAGCAG 
CAGCTGGAGG AGATTCGTAA GCGGGAGGCG GAGGAGCGAG CAAAGCAGGA GGAGGAGAGG 
AGGTGGAGGG AGGAGGAGAG AGCCAAGCGA GAGGCCGAGG AGAAGAGGAG ACAGGAGGAG 
GAGTACTACA CTCGCCTGGA GGCGGAGCGG CGCCGGCAGC ACGACGAGGC CGAGAGGAAG 
CTGCTGACGC CCGACGACCC GGCCGGTCTG TATCGGCCCC CGCTGCCCCG GGATTACCAA 
ACCCCGCCCC CCCAACAAAC CTATAAAACC CCCCCGCCCC CCCCACAGAG AAACACCTCC 
TACCTCAAAA CGCAGGTCAC CTCCCCCGAC ACGCTCTACA CCGCCAAGTT TGTCTCCTAC 
GCGGGCGACG AGGAGGAAGA CGAGGAGGCA GGGAGTGGCG GCGGCGGCGG CAGCAGCAGC 
CAATCAGGTC AGGTGAAGCT GTCGGCGACC CGGAAGTCGT ACGGCGACCT CCCGGCCTCC 
GCCTCGCCCA ATCACAGCAG GCCTCAGCCG CCGCCCACTG CGCGCAAGCC CCGCCCTGTT 
TCTGACGGCA TCTTCCTGTC TGGCTCGTTC CAGCCGCCAT TCAACAACAA CTCCAACAGT 
ACAGGTCCCC CCCTTCCTGC CAAACCCAGC TCCATCCCCC CGGGAGGCTT TAAAGGATTT 
AACTCGTACA CTGGAAACTC AGCAGGAGTT GTAGGATCAG GAGAAGTCTA CAAGGACCCG 
CGAGACAAAT GGACGAAGAG TCAGGAGCAG GAGAACTCGA ACCCCAGCGG CCCTCCGGAG 
AATCTGACCT TCAAGGAGCG GCAGCGTCTC TTCTCTCAGG GGAAGGAGGT TTCCAACAAG 
GTCAAAGCTT CGCGCAAACT CATGGAGCTG GAGAACGAGC TCAACACCAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afdn ( XM_027284627.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_027140428.1 Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X7, mRNA.

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CloneID	OLa200709
Clone ID Related Accession (Same CDS sequence)	XM_027284627.1
Accession Version	XM_027284627.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	afadin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040021.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGCA GCAGGGAGGA GGAGAGGAGG AAACTGGCCG ACATCATCAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CGGCCCACAG AGGACCTGGA GTTCCATGGC 
GTGATGCGGT TCTACTTCCA GGACCGGGTC GCCGGTAACT TTGCCACCAA ATGCATCAGA 
GTGTCGAGCA CGGCGACAAC GCAGGACGTC ATCGAGACGT TAGCAGAGAA GTTCAGACCG 
GACATGAGGA TGCTCTCCTC GCCAAAATAC TCTTTGTACG AGGTCCACGT CAGTGGCGAA 
GAGCGTCAGC TGGATCTGGA CGAGAAGCCT CTGGTGGTTC AGCTGAACTG GAACAAAGAC 
GACAGAGAAG GACGCTTCGT CCTGAAGAAC GAGAACGACA TCCTCCCCAA GAAGAGTCAG 
AGTAACGGCC CGGAGAAGGA GAAGGACGGA GTGATCCAGA ACTTCAAGAG GACTTTGTCC 
AAGAAGGAGA AGAAGAAGGA GAAGAAGAGG GAGAAGGAGT TCGCCCGGAT CCCCGACGGA 
GACGAGCAGG CGCTCAGCCG AGAGGACGGG GAGAACAGTC GTCTGGCGGC GGAGGTTTAC 
AAGGACATGC CGGAGAACAG CTTCACCAGG ACGATCTCCA ACCCGGAGGT GGTGATGAAG 
AGGAGACGCC AGCAGAAGCT GGAGAAACGC ATGCAGGAGT TCATGAGCAG CGACGGGAGG 
CCCGACTCAG GGGGCACGTT GCGGATATAC GCCGACAGCC TGAAGCCGAA CATCCCGTAT 
AAAACCATCC TGCTGTCCAC GAGAGACACG GCTGACTTTG CGGTGGTGGA GGCGCTGGAG 
AAGTACGGGC TGGAGAAGGA GAACCCCCGA GAGTACTGCA TCGCCCGGCA AGACGATAAG 
TCCGGCAAGG AGGCGATCCT GGACGATACC GAGTGTCCAC TGCAGATCTT CAGGGACTGG 
CCTGCAGACA GAGGAGCGCT AGTGTTCCAG CTGAAGAAGA GACCCCCGGA CTACCAGGGC 
AGGAAGGGGA GGAAAGTGGA CGACAAGGGC CTGCGAGGGA AGGACGGCAG CAGCTCCCTG 
CCGCCCGAGA AACTGCCTTA CCTGGTGGAG CTGAGCCCAG GGCGAGGGAA TCACTATGCC 
TACTACGCCT ATCGGCACCA CGAAGACGGG TCTGACTCAC GGGACAAACC AAAGCTGTAC 
CGGCTCCAGC ACAGCATCAC TGAGGTCGGC TCAGACTGCA CAGAGGACGG AGCCATTCAG 
CTGCTCGGTC CGGGGATTCT GCCCCACCAC TGCAACCTCA TGCACAGTGA AGGCATGGTG 
ACGGTGACGC CGCACGGCCC CGACGCCGAC ACCTTCGTCG ACGGGCAACG GGTCACTGAG 
ACGACGGTGC TGCGCTCGGG CTCCACCCTC CAGTTTGGCC AAGCGCACGT CTTCAAGTTT 
GTGGACCCGA TGTTCGACCA GGGAGGGAAG AGAGAGCCGG CCGCCATGAT GAGGAGTCGA 
CACAAGTCCG GCAGCGTACC CGAAACCACC TTCGACCTTC ACGGAGACGT CCACAGCGGA 
GCCGCGCTGC CTACCAGCAA GAGCTCAGGA AAGCTGGAGA TGGAGAGAGG AATGGTCAAA 
CCGATGATCA GAGGAGAGCA GCAGGACGGC AGGTCTCAGG ACATTTCAGC AGACAGAGCC 
GAGTTGACTC TGCCAGCCGG CATCGAGTTC AGAGACAACT CTGAAGACAC CTTCCTGTCG 
GCCATCATCA ACTACACCAA CAGCTCCACG GTTCACTTCA AACTCTCGCC CACGTACGTC 
CTGTACATGG CCTGCCGCTA CATCCTGTCG CCCTCGTACC GGCCCGACAT GTCGCCGTCC 
GAGAGGACGC ACAAGGTCAT CGCCATCGTC AACAAGATGG TGAGCATGAT GGAGGGAGTC 
ATCCAGGAGA TTCCTGAAGG GGATCAGAAG CAGAAGAACA TCGCGGGAGC GCTGGCTTTC 
TGGATGGCCA ACGCCTCCGA GCTGCTCAAC TTCATCAAGC AGGACCGAGA CCTGAACCGC 
ATCACGCTGG ACGCCCAGGA CATCCTCGCC CATCTGGTTC AGATGGCTTT CAAGTACCTG 
GTCCACTGTC TGCAGGCTGA TCTGAATAAC TACATGCCGG CCTTCCTGGA CGACCCCGAG 
GAACACAACC CACAGAGACC CAAGATCGAG GACGTCCTGC ACACCCTCAC GGGGGCGATG 
TCGTTACTGC GGCGGTGTCG GGTCAACGCC GCTCTCACCA TCCAGCTCTT CTCGCAGCTC 
TTCCACTTCA TCAACATGTG GCTCTTCAAC AAGCTGGTGA CGGACACCGA GTCCGGGCTG 
TGCTGTCACT ACTGGGGGGC CATCCTGCGG CAGCAGCTCA GCCACATCGA GGCCTGGGCG 
GAGAAACAGG GGCTGGAGCT CGCCGCCGAC TGCCACCTCA GTCGAATCGT GCAGGCCACC 
ACCCTGCTGA CCATGGACAA ATACTCCATG CAGGATGTGC CGAACATCCA CAACACCTGC 
TTCAAGCTCA ACTCGCTGCA GCTCCACGCC CTCATGAACA ACTACCACTG CGCTCCGGAC 
GAGCCGTACA TCCCGCCGGA GCTGATAGAC CACGTGGTGG CAGTGGCAGA GAACACAGCA 
GATGAGCTGG CGAGGAGCGA CGGCAGAGAG GTTCAGCTGG AGGAGGATCC GGACCTCCAG 
CTGCCGTTCC TCCTTCCCGA GGACGGTTAC TCCTGCGACG TGGTGCGCAG CCTCCCCAAC 
GGCCTGCAGG ACTTCCTGGA GCCGCTGCTG CAGAGAGGTT TCTGTCGGTT GGTACCTCAC 
CCTCGCTCTC CCGGTACCTG GACGGTCCAT TTTGAAGGAG CTGACTGCGA CAGCCACTTC 
TCTGCTGCTG ACAACTCAGA GATGCCAATG AGGAAAGAGC CGGAGGTTGT CACGGTAACC 
CTGAAGAAGC ACAACGGCAT GGGCCTCAGC ATCGTGGCTG CCAAGGGTGC TGGTCAGGAG 
AAGCTGGGCA TCTACATCAA GTCTGTCGTC AAAGGAGGAG CAGCTGATGT GGACGGGCGT 
CTGGCTGCAG GTGACCAGCT GTTGAGCGTG GACGGGCGGA GTCTGGTCGG CCTATCACAA 
GAGAGGGCAG CGGAGCTGAT GACGAGGACG GGCTCAGTCG TGACTCTGGA GGTCGCCAAG 
CAGGGAGCCA TTTACCACGG ACTGGCCACG CTCCTCAACC AGCCGAGCCC CATGATGCCA 
CGAGCGTCAG ACAGAGGCCG CGACAAGAAC GGGAAACTCC GACCAAAGAG CGAAGGCTTC 
GAGTTGTACA ACAGCTCGGT GCAGAACGGC TCTCCGGAGA GCCCGCAGGC CGGCTGGGAC 
TCTTACCCCG AACCAAAGAA GATGAGCGGC GAAGATCGAC TCCTGAAGAA CCGAGCCGAC 
CACCGCTCCA GCCCCAACGT CGCCAATCAG GGCCAGAGTC CTGCAGGCAA AGCAGTGTAC 
CCCGGAGGAC CTGGCACCAA GATCACCTCC GTCTCCACCG GCAACCTGTG CGCAGACGAT 
GAGCCCTCCC CTCCACGTCC AGAAGCTTAC CCCATCCCCA CCCAGACCTA CCCCAGGGAG 
TACTTCACCA TCCCGGCTTC CAAGAGCCAG GATCGGGTCG TTGGTCCTGG GCAGGGACAG 
CACTGGCAGG GCATGGAGGA CAGAGAACGC CTCCCTGTGG TCGACAACAT CCACAACAGC 
ATGCAGCGGG TTAACCACTC CCAGGAGGAG TTGTACCCTC CACCCGGAGG CCTGAGGCCG 
GAGGAGCGCA TGCAGCAGCA CTACCAGCAG GACTACCAGC ACCGCGACAT GGACTACCAG 
CACAGAGAGC TCGACTACCA GAGAGGAGGT CCACCAGGTG TCGGAGGTCC CGATCACTGG 
ACTCCACAGC CTCAGGTGTC CTCCTCGCTG GAGTCGTCCA CGTCCAGCCA GGAGCACCTC 
AACTTCTCCG CCTCCTCCTC GTCCTCCAAC AAAACCCAGA ACCAGAAGAC CGGGCCGGGC 
CGGTGGAAGA CTCCCAACGC CCCTCACTCC GTGCCGCCAC ATTCAGCCCA GCCGCCCTCC 
CGCTCCGACC TGCCCCCTCC TCCGCCTCCA CCGCCAGCTA CTTACCCCGA ATCCTACGAC 
CCATACGACC CCCAACCGGA CCTGCCGCTC CCGCCGCCTC CCTCCGCCGT CACTCCTGCG 
GTAGCTCAGC AGGCGGCCGA CCGCAAGAAA CGTGAGGAGC AGCAGCGCTG GTATGAGAAG 
GAGAAAGCTC GGCTGGAGGA GGAGAGGGAG AGGAAGAGGA GAGAGCAGGA GAGGAAGCTG 
GGTCAGATCC GGGCCAACCC CGTCATGCCC GTCCAGCCTC ACAACAACGG AGGATCCATG 
CCTCCGCCTC ACCACCAGCC GCCTCTGCCG TCTGCAGCCC CGCCTCAGCC CTACCTTCCC 
CCCGCTCAGC CCCAGCCTCC ACCATCCAGA CCGGAGAAGC TGACCAGCCT GCCGCGGTCA 
GCCGTTCCTC CACCCGCCAG CGCCTCCAAC CCTGCAGGCA TGGACACGGT GATCAGGGAG 
CTGTTGCCAC AGCAGCAGCC GCGGACCATC GAGAGGCGAG ACCTGCAGTA CATCACCATC 
AGCAAGGAGG AGCTCACCTC CAGCGACAGT CTGTCTCCAG ACCCCTGGAA GCGAGACGCC 
CGCGAGAAGG CCGAGAAGCA GCAGCAGCTC CACATCGTCG ACCTGCTGGA CAAGGAGATC 
CAGGAGCTCC AGGCGAAGCC GGAGCGGACG GCGGAGGAGA GCGACCGCCT CAGGAAGCTG 
ATGCTGGAGT GGCAGTTCCA GAAACGACTG CAGGAGTCCA AGCAGAGCGA CGAAGACGAG 
GAGGAGGAGG ACGACGAGGA CGTGGACACC ATGATGATCA TGCAGAGGCT GGAGGCCGAG 
AAGAGAGCCA GGTTGCAGGA CGAGGAGCGG CGGCGTAAGC AGCAGCTGGA GGAGATTCGT 
AAGCGGGAGG CGGAGGAGCG AGCAAAGCAG GAGGAGGAGA GGAGGTGGAG GGAGGAGGAG 
AGAGCCAAGC GAGAGGCCGA GGAGAAGAGG AGACAGGAGG AGGAGTACTA CACTCGCCTG 
GAGGCGGAGC GGCGCCGGCA GCACGACGAG GCCGAGAGGA AGCTGCTGAC GCCCGACGAC 
CCGGCCGGTC TGTATCGGCC CCCGCTGCCC CGGGATTACC AAACCCCGCC CCCCCAACAA 
ACCTATAAAA CCCCCCCGCC CCCCCCACAG AGAAACACCT CCTACCTCAA AACGCAGGTC 
ACCTCCCCCG ACACGCTCTA CACCGCCAAG TTTGTCTCCT ACGCGGGCGA CGAGGAGGAA 
GACGAGGAGG CAGGGAGTGG CGGCGGCGGC GGCAGCAGCA GCCAATCAGG TCAGGTGAAG 
CTGTCGGCGA CCCGGAAGTC GTACGGCGAC CTCCCGGCCT CCGCCTCGCC CAATCACAGC 
AGGCCTCAGC CGCCGCCCAC TGCGCGCAAG CCCCGCCCTG TTTCTGACGG CATCTTCCTG 
TCTGGCTCGT TCCAGCCGCC ATTCAACAAC AACTCCAACA GTACAGGTCC CCCCCTTCCT 
GCCAAACCCA GCTCCATCCC CCCGGGAGGC TTTAAAGGAT TTAACTCGTA CACTGGAAAC 
TCAGCAGGAG TTGTAGGATC AGGAGAAGTC TACAAGGACC CGCGAGACAA ATGGACGAAG 
AGTCAGGAGC AGGAGAACTC GAACCCCAGC GGCCCTCCGG AGAATCTGAC CTTCAAGGAG 
CGGCAGCGTC TCTTCTCTCA GGGGAAGGAG GTTTCCAACA AGGTCAAAGC TTCGCGCAAA 
CTCATGGAGC TGGAGAACGA GCTCAACACC AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027140428.1
CDS	233..6088
Translation	MSGSREEERRKLADIINHWNANRLDLFEISRPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERQLDLDEKPLVVQLNWNKDDREGRFVLKNENDILPKKSQSNGPEKEKDGVIQNFKRTLSKKEKKKEKKREKEFARIPDGDEQALSREDGENSRLAAEVYKDMPENSFTRTISNPEVVMKRRRQQKLEKRMQEFMSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTRDTADFAVVEALEKYGLEKENPREYCIARQDDKSGKEAILDDTECPLQIFRDWPADRGALVFQLKKRPPDYQGRKGRKVDDKGLRGKDGSSSLPPEKLPYLVELSPGRGNHYAYYAYRHHEDGSDSRDKPKLYRLQHSITEVGSDCTEDGAIQLLGPGILPHHCNLMHSEGMVTVTPHGPDADTFVDGQRVTETTVLRSGSTLQFGQAHVFKFVDPMFDQGGKREPAAMMRSRHKSGSVPETTFDLHGDVHSGAALPTSKSSGKLEMERGMVKPMIRGEQQDGRSQDISADRAELTLPAGIEFRDNSEDTFLSAIINYTNSSTVHFKLSPTYVLYMACRYILSPSYRPDMSPSERTHKVIAIVNKMVSMMEGVIQEIPEGDQKQKNIAGALAFWMANASELLNFIKQDRDLNRITLDAQDILAHLVQMAFKYLVHCLQADLNNYMPAFLDDPEEHNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVTDTESGLCCHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVPNIHNTCFKLNSLQLHALMNNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRSLPNGLQDFLEPLLQRGFCRLVPHPRSPGTWTVHFEGADCDSHFSAADNSEMPMRKEPEVVTVTLKKHNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRDKNGKLRPKSEGFELYNSSVQNGSPESPQAGWDSYPEPKKMSGEDRLLKNRADHRSSPNVANQGQSPAGKAVYPGGPGTKITSVSTGNLCADDEPSPPRPEAYPIPTQTYPREYFTIPASKSQDRVVGPGQGQHWQGMEDRERLPVVDNIHNSMQRVNHSQEELYPPPGGLRPEERMQQHYQQDYQHRDMDYQHRELDYQRGGPPGVGGPDHWTPQPQVSSSLESSTSSQEHLNFSASSSSSNKTQNQKTGPGRWKTPNAPHSVPPHSAQPPSRSDLPPPPPPPPATYPESYDPYDPQPDLPLPPPPSAVTPAVAQQAADRKKREEQQRWYEKEKARLEEERERKRREQERKLGQIRANPVMPVQPHNNGGSMPPPHHQPPLPSAAPPQPYLPPAQPQPPPSRPEKLTSLPRSAVPPPASASNPAGMDTVIRELLPQQQPRTIERRDLQYITISKEELTSSDSLSPDPWKRDAREKAEKQQQLHIVDLLDKEIQELQAKPERTAEESDRLRKLMLEWQFQKRLQESKQSDEDEEEEDDEDVDTMMIMQRLEAEKRARLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERAKREAEEKRRQEEEYYTRLEAERRRQHDEAERKLLTPDDPAGLYRPPLPRDYQTPPPQQTYKTPPPPPQRNTSYLKTQVTSPDTLYTAKFVSYAGDEEEDEEAGSGGGGGSSSQSGQVKLSATRKSYGDLPASASPNHSRPQPPPTARKPRPVSDGIFLSGSFQPPFNNNSNSTGPPLPAKPSSIPPGGFKGFNSYTGNSAGVVGSGEVYKDPRDKWTKSQEQENSNPSGPPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNTK

Target ORF information:

RefSeq Version	XM_027284627.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027284627.1

ORF Insert Sequence:

ATGTCGGGCA GCAGGGAGGA GGAGAGGAGG AAACTGGCCG ACATCATCAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CGGCCCACAG AGGACCTGGA GTTCCATGGC 
GTGATGCGGT TCTACTTCCA GGACCGGGTC GCCGGTAACT TTGCCACCAA ATGCATCAGA 
GTGTCGAGCA CGGCGACAAC GCAGGACGTC ATCGAGACGT TAGCAGAGAA GTTCAGACCG 
GACATGAGGA TGCTCTCCTC GCCAAAATAC TCTTTGTACG AGGTCCACGT CAGTGGCGAA 
GAGCGTCAGC TGGATCTGGA CGAGAAGCCT CTGGTGGTTC AGCTGAACTG GAACAAAGAC 
GACAGAGAAG GACGCTTCGT CCTGAAGAAC GAGAACGACA TCCTCCCCAA GAAGAGTCAG 
AGTAACGGCC CGGAGAAGGA GAAGGACGGA GTGATCCAGA ACTTCAAGAG GACTTTGTCC 
AAGAAGGAGA AGAAGAAGGA GAAGAAGAGG GAGAAGGAGT TCGCCCGGAT CCCCGACGGA 
GACGAGCAGG CGCTCAGCCG AGAGGACGGG GAGAACAGTC GTCTGGCGGC GGAGGTTTAC 
AAGGACATGC CGGAGAACAG CTTCACCAGG ACGATCTCCA ACCCGGAGGT GGTGATGAAG 
AGGAGACGCC AGCAGAAGCT GGAGAAACGC ATGCAGGAGT TCATGAGCAG CGACGGGAGG 
CCCGACTCAG GGGGCACGTT GCGGATATAC GCCGACAGCC TGAAGCCGAA CATCCCGTAT 
AAAACCATCC TGCTGTCCAC GAGAGACACG GCTGACTTTG CGGTGGTGGA GGCGCTGGAG 
AAGTACGGGC TGGAGAAGGA GAACCCCCGA GAGTACTGCA TCGCCCGGCA AGACGATAAG 
TCCGGCAAGG AGGCGATCCT GGACGATACC GAGTGTCCAC TGCAGATCTT CAGGGACTGG 
CCTGCAGACA GAGGAGCGCT AGTGTTCCAG CTGAAGAAGA GACCCCCGGA CTACCAGGGC 
AGGAAGGGGA GGAAAGTGGA CGACAAGGGC CTGCGAGGGA AGGACGGCAG CAGCTCCCTG 
CCGCCCGAGA AACTGCCTTA CCTGGTGGAG CTGAGCCCAG GGCGAGGGAA TCACTATGCC 
TACTACGCCT ATCGGCACCA CGAAGACGGG TCTGACTCAC GGGACAAACC AAAGCTGTAC 
CGGCTCCAGC ACAGCATCAC TGAGGTCGGC TCAGACTGCA CAGAGGACGG AGCCATTCAG 
CTGCTCGGTC CGGGGATTCT GCCCCACCAC TGCAACCTCA TGCACAGTGA AGGCATGGTG 
ACGGTGACGC CGCACGGCCC CGACGCCGAC ACCTTCGTCG ACGGGCAACG GGTCACTGAG 
ACGACGGTGC TGCGCTCGGG CTCCACCCTC CAGTTTGGCC AAGCGCACGT CTTCAAGTTT 
GTGGACCCGA TGTTCGACCA GGGAGGGAAG AGAGAGCCGG CCGCCATGAT GAGGAGTCGA 
CACAAGTCCG GCAGCGTACC CGAAACCACC TTCGACCTTC ACGGAGACGT CCACAGCGGA 
GCCGCGCTGC CTACCAGCAA GAGCTCAGGA AAGCTGGAGA TGGAGAGAGG AATGGTCAAA 
CCGATGATCA GAGGAGAGCA GCAGGACGGC AGGTCTCAGG ACATTTCAGC AGACAGAGCC 
GAGTTGACTC TGCCAGCCGG CATCGAGTTC AGAGACAACT CTGAAGACAC CTTCCTGTCG 
GCCATCATCA ACTACACCAA CAGCTCCACG GTTCACTTCA AACTCTCGCC CACGTACGTC 
CTGTACATGG CCTGCCGCTA CATCCTGTCG CCCTCGTACC GGCCCGACAT GTCGCCGTCC 
GAGAGGACGC ACAAGGTCAT CGCCATCGTC AACAAGATGG TGAGCATGAT GGAGGGAGTC 
ATCCAGGAGA TTCCTGAAGG GGATCAGAAG CAGAAGAACA TCGCGGGAGC GCTGGCTTTC 
TGGATGGCCA ACGCCTCCGA GCTGCTCAAC TTCATCAAGC AGGACCGAGA CCTGAACCGC 
ATCACGCTGG ACGCCCAGGA CATCCTCGCC CATCTGGTTC AGATGGCTTT CAAGTACCTG 
GTCCACTGTC TGCAGGCTGA TCTGAATAAC TACATGCCGG CCTTCCTGGA CGACCCCGAG 
GAACACAACC CACAGAGACC CAAGATCGAG GACGTCCTGC ACACCCTCAC GGGGGCGATG 
TCGTTACTGC GGCGGTGTCG GGTCAACGCC GCTCTCACCA TCCAGCTCTT CTCGCAGCTC 
TTCCACTTCA TCAACATGTG GCTCTTCAAC AAGCTGGTGA CGGACACCGA GTCCGGGCTG 
TGCTGTCACT ACTGGGGGGC CATCCTGCGG CAGCAGCTCA GCCACATCGA GGCCTGGGCG 
GAGAAACAGG GGCTGGAGCT CGCCGCCGAC TGCCACCTCA GTCGAATCGT GCAGGCCACC 
ACCCTGCTGA CCATGGACAA ATACTCCATG CAGGATGTGC CGAACATCCA CAACACCTGC 
TTCAAGCTCA ACTCGCTGCA GCTCCACGCC CTCATGAACA ACTACCACTG CGCTCCGGAC 
GAGCCGTACA TCCCGCCGGA GCTGATAGAC CACGTGGTGG CAGTGGCAGA GAACACAGCA 
GATGAGCTGG CGAGGAGCGA CGGCAGAGAG GTTCAGCTGG AGGAGGATCC GGACCTCCAG 
CTGCCGTTCC TCCTTCCCGA GGACGGTTAC TCCTGCGACG TGGTGCGCAG CCTCCCCAAC 
GGCCTGCAGG ACTTCCTGGA GCCGCTGCTG CAGAGAGGTT TCTGTCGGTT GGTACCTCAC 
CCTCGCTCTC CCGGTACCTG GACGGTCCAT TTTGAAGGAG CTGACTGCGA CAGCCACTTC 
TCTGCTGCTG ACAACTCAGA GATGCCAATG AGGAAAGAGC CGGAGGTTGT CACGGTAACC 
CTGAAGAAGC ACAACGGCAT GGGCCTCAGC ATCGTGGCTG CCAAGGGTGC TGGTCAGGAG 
AAGCTGGGCA TCTACATCAA GTCTGTCGTC AAAGGAGGAG CAGCTGATGT GGACGGGCGT 
CTGGCTGCAG GTGACCAGCT GTTGAGCGTG GACGGGCGGA GTCTGGTCGG CCTATCACAA 
GAGAGGGCAG CGGAGCTGAT GACGAGGACG GGCTCAGTCG TGACTCTGGA GGTCGCCAAG 
CAGGGAGCCA TTTACCACGG ACTGGCCACG CTCCTCAACC AGCCGAGCCC CATGATGCCA 
CGAGCGTCAG ACAGAGGCCG CGACAAGAAC GGGAAACTCC GACCAAAGAG CGAAGGCTTC 
GAGTTGTACA ACAGCTCGGT GCAGAACGGC TCTCCGGAGA GCCCGCAGGC CGGCTGGGAC 
TCTTACCCCG AACCAAAGAA GATGAGCGGC GAAGATCGAC TCCTGAAGAA CCGAGCCGAC 
CACCGCTCCA GCCCCAACGT CGCCAATCAG GGCCAGAGTC CTGCAGGCAA AGCAGTGTAC 
CCCGGAGGAC CTGGCACCAA GATCACCTCC GTCTCCACCG GCAACCTGTG CGCAGACGAT 
GAGCCCTCCC CTCCACGTCC AGAAGCTTAC CCCATCCCCA CCCAGACCTA CCCCAGGGAG 
TACTTCACCA TCCCGGCTTC CAAGAGCCAG GATCGGGTCG TTGGTCCTGG GCAGGGACAG 
CACTGGCAGG GCATGGAGGA CAGAGAACGC CTCCCTGTGG TCGACAACAT CCACAACAGC 
ATGCAGCGGG TTAACCACTC CCAGGAGGAG TTGTACCCTC CACCCGGAGG CCTGAGGCCG 
GAGGAGCGCA TGCAGCAGCA CTACCAGCAG GACTACCAGC ACCGCGACAT GGACTACCAG 
CACAGAGAGC TCGACTACCA GAGAGGAGGT CCACCAGGTG TCGGAGGTCC CGATCACTGG 
ACTCCACAGC CTCAGGTGTC CTCCTCGCTG GAGTCGTCCA CGTCCAGCCA GGAGCACCTC 
AACTTCTCCG CCTCCTCCTC GTCCTCCAAC AAAACCCAGA ACCAGAAGAC CGGGCCGGGC 
CGGTGGAAGA CTCCCAACGC CCCTCACTCC GTGCCGCCAC ATTCAGCCCA GCCGCCCTCC 
CGCTCCGACC TGCCCCCTCC TCCGCCTCCA CCGCCAGCTA CTTACCCCGA ATCCTACGAC 
CCATACGACC CCCAACCGGA CCTGCCGCTC CCGCCGCCTC CCTCCGCCGT CACTCCTGCG 
GTAGCTCAGC AGGCGGCCGA CCGCAAGAAA CGTGAGGAGC AGCAGCGCTG GTATGAGAAG 
GAGAAAGCTC GGCTGGAGGA GGAGAGGGAG AGGAAGAGGA GAGAGCAGGA GAGGAAGCTG 
GGTCAGATCC GGGCCAACCC CGTCATGCCC GTCCAGCCTC ACAACAACGG AGGATCCATG 
CCTCCGCCTC ACCACCAGCC GCCTCTGCCG TCTGCAGCCC CGCCTCAGCC CTACCTTCCC 
CCCGCTCAGC CCCAGCCTCC ACCATCCAGA CCGGAGAAGC TGACCAGCCT GCCGCGGTCA 
GCCGTTCCTC CACCCGCCAG CGCCTCCAAC CCTGCAGGCA TGGACACGGT GATCAGGGAG 
CTGTTGCCAC AGCAGCAGCC GCGGACCATC GAGAGGCGAG ACCTGCAGTA CATCACCATC 
AGCAAGGAGG AGCTCACCTC CAGCGACAGT CTGTCTCCAG ACCCCTGGAA GCGAGACGCC 
CGCGAGAAGG CCGAGAAGCA GCAGCAGCTC CACATCGTCG ACCTGCTGGA CAAGGAGATC 
CAGGAGCTCC AGGCGAAGCC GGAGCGGACG GCGGAGGAGA GCGACCGCCT CAGGAAGCTG 
ATGCTGGAGT GGCAGTTCCA GAAACGACTG CAGGAGTCCA AGCAGAGCGA CGAAGACGAG 
GAGGAGGAGG ACGACGAGGA CGTGGACACC ATGATGATCA TGCAGAGGCT GGAGGCCGAG 
AAGAGAGCCA GGTTGCAGGA CGAGGAGCGG CGGCGTAAGC AGCAGCTGGA GGAGATTCGT 
AAGCGGGAGG CGGAGGAGCG AGCAAAGCAG GAGGAGGAGA GGAGGTGGAG GGAGGAGGAG 
AGAGCCAAGC GAGAGGCCGA GGAGAAGAGG AGACAGGAGG AGGAGTACTA CACTCGCCTG 
GAGGCGGAGC GGCGCCGGCA GCACGACGAG GCCGAGAGGA AGCTGCTGAC GCCCGACGAC 
CCGGCCGGTC TGTATCGGCC CCCGCTGCCC CGGGATTACC AAACCCCGCC CCCCCAACAA 
ACCTATAAAA CCCCCCCGCC CCCCCCACAG AGAAACACCT CCTACCTCAA AACGCAGGTC 
ACCTCCCCCG ACACGCTCTA CACCGCCAAG TTTGTCTCCT ACGCGGGCGA CGAGGAGGAA 
GACGAGGAGG CAGGGAGTGG CGGCGGCGGC GGCAGCAGCA GCCAATCAGG TCAGGTGAAG 
CTGTCGGCGA CCCGGAAGTC GTACGGCGAC CTCCCGGCCT CCGCCTCGCC CAATCACAGC 
AGGCCTCAGC CGCCGCCCAC TGCGCGCAAG CCCCGCCCTG TTTCTGACGG CATCTTCCTG 
TCTGGCTCGT TCCAGCCGCC ATTCAACAAC AACTCCAACA GTACAGGTCC CCCCCTTCCT 
GCCAAACCCA GCTCCATCCC CCCGGGAGGC TTTAAAGGAT TTAACTCGTA CACTGGAAAC 
TCAGCAGGAG TTGTAGGATC AGGAGAAGTC TACAAGGACC CGCGAGACAA ATGGACGAAG 
AGTCAGGAGC AGGAGAACTC GAACCCCAGC GGCCCTCCGG AGAATCTGAC CTTCAAGGAG 
CGGCAGCGTC TCTTCTCTCA GGGGAAGGAG GTTTCCAACA AGGTCAAAGC TTCGCGCAAA 
CTCATGGAGC TGGAGAACGA GCTCAACACC AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afdn ( XM_027284628.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_027140429.1 Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OLa200710
Clone ID Related Accession (Same CDS sequence)	XM_027284628.1
Accession Version	XM_027284628.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5850bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	afadin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040021.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGCA GCAGGGAGGA GGAGAGGAGG AAACTGGCCG ACATCATCAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CGGCCCACAG AGGACCTGGA GTTCCATGGC 
GTGATGCGGT TCTACTTCCA GGACCGGGTC GCCGGTAACT TTGCCACCAA ATGCATCAGA 
GTGTCGAGCA CGGCGACAAC GCAGGACGTC ATCGAGACGT TAGCAGAGAA GTTCAGACCG 
GACATGAGGA TGCTCTCCTC GCCAAAATAC TCTTTGTACG AGGTCCACGT CAGTGGCGAA 
GAGCGTCAGC TGGATCTGGA CGAGAAGCCT CTGGTGGTTC AGCTGAACTG GAACAAAGAC 
GACAGAGAAG GACGCTTCGT CCTGAAGAAC GAGAACGACA TCCTCCCCAA GAAGAGTCAG 
AGTAACGGCC CGGAGAAGGA GAAGGACGGA GTGATCCAGA ACTTCAAGAG GACTTTGTCC 
AAGAAGGAGA AGAAGAAGGA GAAGAAGAGG GAGAAGGAGT TCGCCCGGAT CCCCGACGGA 
GACGAGCAGG CGCTCAGCCG AGAGGACGGG GAGAACAGTC GTCTGGCGGC GGAGGTTTAC 
AAGGACATGC CGGAGAACAG CTTCACCAGG ACGATCTCCA ACCCGGAGGT GGTGATGAAG 
AGGAGACGCC AGCAGAAGCT GGAGAAACGC ATGCAGGAGT TCATGAGCAG CGACGGGAGG 
CCCGACTCAG GGGGCACGTT GCGGATATAC GCCGACAGCC TGAAGCCGAA CATCCCGTAT 
AAAACCATCC TGCTGTCCAC GAGAGACACG GCTGACTTTG CGGTGGTGGA GGCGCTGGAG 
AAGTACGGGC TGGAGAAGGA GAACCCCCGA GAGTACTGCA TCGCCCGGCA AGACGATAAG 
TCCGGCAAGG AGGCGATCCT GGACGATACC GAGTGTCCAC TGCAGATCTT CAGGGACTGG 
CCTGCAGACA GAGGAGCGCT AGTGTTCCAG CTGAAGAAGA GACCCCCGGA CTACCAGGGC 
AGGAAGGGGA GGAAAGTGGA CGACAAGGGC CTGCGAGGGA AGGACGGCAG CAGCTCCCTG 
CCGCCCGAGA AACTGCCTTA CCTGGTGGAG CTGAGCCCAG ACGGGTCTGA CTCACGGGAC 
AAACCAAAGC TGTACCGGCT CCAGCACAGC ATCACTGAGG TCGGCTCAGA CTGCACAGAG 
GACGGAGCCA TTCAGCTGCT CGGTCCGGGG ATTCTGCCCC ACCACTGCAA CCTCATGCAC 
AGTGAAGGCA TGGTGACGGT GACGCCGCAC GGCCCCGACG CCGACACCTT CGTCGACGGG 
CAACGGGTCA CTGAGACGAC GGTGCTGCGC TCGGGCTCCA CCCTCCAGTT TGGCCAAGCG 
CACGTCTTCA AGTTTGTGGA CCCGATGTTC GACCAGGGAG GGAAGAGAGA GCCGGCCGCC 
ATGATGAGGA GTCGACACAA GTCCGGCAGC GTACCCGAAA CCACCTTCGA CCTTCACGGA 
GACGTCCACA GCGGAGCCGC GCTGCCTACC AGCAAGAGCT CAGGAAAGCT GGAGATGGAG 
AGAGGAATGG TCAAACCGAT GATCAGAGGA GAGCAGCAGG ACGGCAGGTC TCAGGACATT 
TCAGCAGACA GAGCCGAGTT GACTCTGCCA GCCGGCATCG AGTTCAGAGA CAACTCTGAA 
GACACCTTCC TGTCGGCCAT CATCAACTAC ACCAACAGCT CCACGGTTCA CTTCAAACTC 
TCGCCCACGT ACGTCCTGTA CATGGCCTGC CGCTACATCC TGTCGCCCTC GTACCGGCCC 
GACATGTCGC CGTCCGAGAG GACGCACAAG GTCATCGCCA TCGTCAACAA GATGGTGAGC 
ATGATGGAGG GAGTCATCCA GGAGATTCCT GAAGGGGATC AGAAGCAGAA GAACATCGCG 
GGAGCGCTGG CTTTCTGGAT GGCCAACGCC TCCGAGCTGC TCAACTTCAT CAAGCAGGAC 
CGAGACCTGA ACCGCATCAC GCTGGACGCC CAGGACATCC TCGCCCATCT GGTTCAGATG 
GCTTTCAAGT ACCTGGTCCA CTGTCTGCAG GCTGATCTGA ATAACTACAT GCCGGCCTTC 
CTGGACGACC CCGAGGAACA CAACCCACAG AGACCCAAGA TCGAGGACGT CCTGCACACC 
CTCACGGGGG CGATGTCGTT ACTGCGGCGG TGTCGGGTCA ACGCCGCTCT CACCATCCAG 
CTCTTCTCGC AGCTCTTCCA CTTCATCAAC ATGTGGCTCT TCAACAAGCT GGTGACGGAC 
ACCGAGTCCG GGCTGTGCTG TCACTACTGG GGGGCCATCC TGCGGCAGCA GCTCAGCCAC 
ATCGAGGCCT GGGCGGAGAA ACAGGGGCTG GAGCTCGCCG CCGACTGCCA CCTCAGTCGA 
ATCGTGCAGG CCACCACCCT GCTGACCATG GACAAATACT CCATGCAGGA TGTGCCGAAC 
ATCCACAACA CCTGCTTCAA GCTCAACTCG CTGCAGCTCC ACGCCCTCAT GAACAACTAC 
CACTGCGCTC CGGACGAGCC GTACATCCCG CCGGAGCTGA TAGACCACGT GGTGGCAGTG 
GCAGAGAACA CAGCAGATGA GCTGGCGAGG AGCGACGGCA GAGAGGTTCA GCTGGAGGAG 
GATCCGGACC TCCAGCTGCC GTTCCTCCTT CCCGAGGACG GTTACTCCTG CGACGTGGTG 
CGCAGCCTCC CCAACGGCCT GCAGGACTTC CTGGAGCCGC TGCTGCAGAG AGGTTTCTGT 
CGGTTGGTAC CTCACCCTCG CTCTCCCGGT ACCTGGACGG TCCATTTTGA AGGAGCTGAC 
TGCGACAGCC ACTTCTCTGC TGCTGACAAC TCAGAGATGC CAATGAGGAA AGAGCCGGAG 
GTTGTCACGG TAACCCTGAA GAAGCACAAC GGCATGGGCC TCAGCATCGT GGCTGCCAAG 
GGTGCTGGTC AGGAGAAGCT GGGCATCTAC ATCAAGTCTG TCGTCAAAGG AGGAGCAGCT 
GATGTGGACG GGCGTCTGGC TGCAGGTGAC CAGCTGTTGA GCGTGGACGG GCGGAGTCTG 
GTCGGCCTAT CACAAGAGAG GGCAGCGGAG CTGATGACGA GGACGGGCTC AGTCGTGACT 
CTGGAGGTCG CCAAGCAGGG AGCCATTTAC CACGGACTGG CCACGCTCCT CAACCAGCCG 
AGCCCCATGA TGCCACGAGC GTCAGACAGA GGCCGCGACA AGAACGGGAA ACTCCGACCA 
AAGAGCGAAG GCTTCGAGTT GTACAACAGC TCGGTGCAGA ACGGCTCTCC GGAGAGCCCG 
CAGGCCGGCT GGGACTCTTA CCCCGAACCA AAGAAGATGA GCGGCGAAGA TCGACTCCTG 
AAGAACCGAG CCGACCACCG CTCCAGCCCC AACGTCGCCA ATCAGGGCCA GAGTCCTGCA 
GGCAAAGCAG TGTACCCCGG AGGACCTGGC ACCAAGATCA CCTCCGTCTC CACCGGCAAC 
CTGTGCGCAG ACGATGAGCC CTCCCCTCCA CGTCCAGAAG CTTACCCCAT CCCCACCCAG 
ACCTACCCCA GGGAGTACTT CACCATCCCG GCTTCCAAGA GCCAGGATCG GGTCGTTGGT 
CCTGGGCAGG GACAGCACTG GCAGGGCATG GAGGACAGAG AACGCCTCCC TGTGGTCGAC 
AACATCCACA ACAGCATGCA GCGGGTTAAC CACTCCCAGG AGGAGTTGTA CCCTCCACCC 
GGAGGCCTGA GGCCGGAGGA GCGCATGCAG CAGCACTACC AGCAGGACTA CCAGCACCGC 
GACATGGACT ACCAGCACAG AGAGCTCGAC TACCAGAGAG GAGGTCCACC AGGTGTCGGA 
GGTCCCGATC ACTGGACTCC ACAGCCTCAG GTGTCCTCCT CGCTGGAGTC GTCCACGTCC 
AGCCAGGAGC ACCTCAACTT CTCCGCCTCC TCCTCGTCCT CCAACAAAAC CCAGAACCAG 
AAGACCGGGC CGGGCCGGTG GAAGACTCCC AACGCCCCTC ACTCCGTGCC GCCACATTCA 
GCCCAGCCGC CCTCCCGCTC CGACCTGCCC CCTCCTCCGC CTCCACCGCC AGCTACTTAC 
CCCGAATCCT ACGACCCATA CGACCCCCAA CCGGACCTGC CGCTCCCGCC GCCTCCCTCC 
GCCGTCACTC CTGCGGTAGC TCAGCAGGCG GCCGACCGCA AGAAACGTGA GGAGCAGCAG 
CGCTGGTATG AGAAGGAGAA AGCTCGGCTG GAGGAGGAGA GGGAGAGGAA GAGGAGAGAG 
CAGGAGAGGA AGCTGGGTCA GATCCGGGCC AACCCCGTCA TGCCCGTCCA GCCTCACAAC 
AACGGAGGAT CCATGCCTCC GCCTCACCAC CAGCCGCCTC TGCCGTCTGC AGCCCCGCCT 
CAGCCCTACC TTCCCCCCGC TCAGCCCCAG CCTCCACCAT CCAGACCGGA GAAGCTGACC 
AGCCTGCCGC GGTCAGCCGT TCCTCCACCC GCCAGCGCCT CCAACCCTGC AGGCATGGAC 
ACGGTGATCA GGGAGCTGTT GCCACAGCAG CAGCCGCGGA CCATCGAGAG GCGAGACCTG 
CAGTACATCA CCATCAGCAA GGAGGAGCTC ACCTCCAGCG ACAGTCTGTC TCCAGACCCC 
TGGAAGCGAG ACGCCCGCGA GAAGGCCGAG AAGCAGCAGC AGCTCCACAT CGTCGACCTG 
CTGGACAAGG AGATCCAGGA GCTCCAGGCG AAGCCGGAGC GGACGGCGGA GGAGAGCGAC 
CGCCTCAGGA AGCTGATGCT GGAGTGGCAG TTCCAGAAAC GACTGCAGGA GTCCAAGCAG 
AGCGACGAAG ACGAGGAGGA GGAGGACGAC GAGGACGTGG ACACCATGAT GATCATGCAG 
AGGCTGGAGG CCGAGAAGAG AGCCAGGCAG CAGAATGCTG TCCCAGCTAT CTCTGTTCTA 
GACTTGTTGC AGGACGAGGA GCGGCGGCGT AAGCAGCAGC TGGAGGAGAT TCGTAAGCGG 
GAGGCGGAGG AGCGAGCAAA GCAGGAGGAG GAGAGGAGGT GGAGGGAGGA GGAGAGAGCC 
AAGCGAGAGG CCGAGGAGAA GAGGAGACAG GAGGAGGAGT ACTACACTCG CCTGGAGGCG 
GAGCGGCGCC GGCAGCACGA CGAGGCCGAG AGGAAGCTGC TGACGCCCGA CGACCCGGCC 
GGTCTGTATC GGCCCCCGCT GCCCCGGGAT TACCAAACCC CGCCCCCCCA ACAAACCTAT 
AAAACCCCCC CGCCCCCCCC ACAGAGAAAC ACCTCCTACC TCAAAACGCA GGTCACCTCC 
CCCGACACGC TCTACACCGC CAAGTTTGTC TCCTACGCGG GCGACGAGGA GGAAGACGAG 
GAGGCAGGGA GTGGCGGCGG CGGCGGCAGC AGCAGCCAAT CAGGTCAGGT GAAGCTGTCG 
GCGACCCGGA AGTCGTACGG CGACCTCCCG GCCTCCGCCT CGCCCAATCA CAGCAGGCCT 
CAGCCGCCGC CCACTGCGCG CAAGCCCCGC CCTGTTTCTG ACGGCATCTT CCTGTCTGGC 
TCGTTCCAGC CGCCATTCAA CAACAACTCC AACAGTACAG GTCCCCCCCT TCCTGCCAAA 
CCCAGCTCCA TCCCCCCGGG AGGCTTTAAA GGATTTAACT CGTACACTGG AAACTCAGCA 
GGAGTTGTAG GATCAGGAGA AGTCTACAAG GACCCGCGAG ACAAATGGAC GAAGAGTCAG 
GAGCAGGAGA ACTCGAACCC CAGCGGCCCT CCGGAGAATC TGACCTTCAA GGAGCGGCAG 
CGTCTCTTCT CTCAGGGGAA GGAGGTTTCC AACAAGGTCA AAGCTTCGCG CAAACTCATG 
GAGCTGGAGA ACGAGCTCAA CACCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027140429.1
CDS	233..6082
Translation	MSGSREEERRKLADIINHWNANRLDLFEISRPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERQLDLDEKPLVVQLNWNKDDREGRFVLKNENDILPKKSQSNGPEKEKDGVIQNFKRTLSKKEKKKEKKREKEFARIPDGDEQALSREDGENSRLAAEVYKDMPENSFTRTISNPEVVMKRRRQQKLEKRMQEFMSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTRDTADFAVVEALEKYGLEKENPREYCIARQDDKSGKEAILDDTECPLQIFRDWPADRGALVFQLKKRPPDYQGRKGRKVDDKGLRGKDGSSSLPPEKLPYLVELSPDGSDSRDKPKLYRLQHSITEVGSDCTEDGAIQLLGPGILPHHCNLMHSEGMVTVTPHGPDADTFVDGQRVTETTVLRSGSTLQFGQAHVFKFVDPMFDQGGKREPAAMMRSRHKSGSVPETTFDLHGDVHSGAALPTSKSSGKLEMERGMVKPMIRGEQQDGRSQDISADRAELTLPAGIEFRDNSEDTFLSAIINYTNSSTVHFKLSPTYVLYMACRYILSPSYRPDMSPSERTHKVIAIVNKMVSMMEGVIQEIPEGDQKQKNIAGALAFWMANASELLNFIKQDRDLNRITLDAQDILAHLVQMAFKYLVHCLQADLNNYMPAFLDDPEEHNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVTDTESGLCCHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVPNIHNTCFKLNSLQLHALMNNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRSLPNGLQDFLEPLLQRGFCRLVPHPRSPGTWTVHFEGADCDSHFSAADNSEMPMRKEPEVVTVTLKKHNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRDKNGKLRPKSEGFELYNSSVQNGSPESPQAGWDSYPEPKKMSGEDRLLKNRADHRSSPNVANQGQSPAGKAVYPGGPGTKITSVSTGNLCADDEPSPPRPEAYPIPTQTYPREYFTIPASKSQDRVVGPGQGQHWQGMEDRERLPVVDNIHNSMQRVNHSQEELYPPPGGLRPEERMQQHYQQDYQHRDMDYQHRELDYQRGGPPGVGGPDHWTPQPQVSSSLESSTSSQEHLNFSASSSSSNKTQNQKTGPGRWKTPNAPHSVPPHSAQPPSRSDLPPPPPPPPATYPESYDPYDPQPDLPLPPPPSAVTPAVAQQAADRKKREEQQRWYEKEKARLEEERERKRREQERKLGQIRANPVMPVQPHNNGGSMPPPHHQPPLPSAAPPQPYLPPAQPQPPPSRPEKLTSLPRSAVPPPASASNPAGMDTVIRELLPQQQPRTIERRDLQYITISKEELTSSDSLSPDPWKRDAREKAEKQQQLHIVDLLDKEIQELQAKPERTAEESDRLRKLMLEWQFQKRLQESKQSDEDEEEEDDEDVDTMMIMQRLEAEKRARQQNAVPAISVLDLLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERAKREAEEKRRQEEEYYTRLEAERRRQHDEAERKLLTPDDPAGLYRPPLPRDYQTPPPQQTYKTPPPPPQRNTSYLKTQVTSPDTLYTAKFVSYAGDEEEDEEAGSGGGGGSSSQSGQVKLSATRKSYGDLPASASPNHSRPQPPPTARKPRPVSDGIFLSGSFQPPFNNNSNSTGPPLPAKPSSIPPGGFKGFNSYTGNSAGVVGSGEVYKDPRDKWTKSQEQENSNPSGPPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNTK

Target ORF information:

RefSeq Version	XM_027284628.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027284628.1

ORF Insert Sequence:

ATGTCGGGCA GCAGGGAGGA GGAGAGGAGG AAACTGGCCG ACATCATCAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CGGCCCACAG AGGACCTGGA GTTCCATGGC 
GTGATGCGGT TCTACTTCCA GGACCGGGTC GCCGGTAACT TTGCCACCAA ATGCATCAGA 
GTGTCGAGCA CGGCGACAAC GCAGGACGTC ATCGAGACGT TAGCAGAGAA GTTCAGACCG 
GACATGAGGA TGCTCTCCTC GCCAAAATAC TCTTTGTACG AGGTCCACGT CAGTGGCGAA 
GAGCGTCAGC TGGATCTGGA CGAGAAGCCT CTGGTGGTTC AGCTGAACTG GAACAAAGAC 
GACAGAGAAG GACGCTTCGT CCTGAAGAAC GAGAACGACA TCCTCCCCAA GAAGAGTCAG 
AGTAACGGCC CGGAGAAGGA GAAGGACGGA GTGATCCAGA ACTTCAAGAG GACTTTGTCC 
AAGAAGGAGA AGAAGAAGGA GAAGAAGAGG GAGAAGGAGT TCGCCCGGAT CCCCGACGGA 
GACGAGCAGG CGCTCAGCCG AGAGGACGGG GAGAACAGTC GTCTGGCGGC GGAGGTTTAC 
AAGGACATGC CGGAGAACAG CTTCACCAGG ACGATCTCCA ACCCGGAGGT GGTGATGAAG 
AGGAGACGCC AGCAGAAGCT GGAGAAACGC ATGCAGGAGT TCATGAGCAG CGACGGGAGG 
CCCGACTCAG GGGGCACGTT GCGGATATAC GCCGACAGCC TGAAGCCGAA CATCCCGTAT 
AAAACCATCC TGCTGTCCAC GAGAGACACG GCTGACTTTG CGGTGGTGGA GGCGCTGGAG 
AAGTACGGGC TGGAGAAGGA GAACCCCCGA GAGTACTGCA TCGCCCGGCA AGACGATAAG 
TCCGGCAAGG AGGCGATCCT GGACGATACC GAGTGTCCAC TGCAGATCTT CAGGGACTGG 
CCTGCAGACA GAGGAGCGCT AGTGTTCCAG CTGAAGAAGA GACCCCCGGA CTACCAGGGC 
AGGAAGGGGA GGAAAGTGGA CGACAAGGGC CTGCGAGGGA AGGACGGCAG CAGCTCCCTG 
CCGCCCGAGA AACTGCCTTA CCTGGTGGAG CTGAGCCCAG ACGGGTCTGA CTCACGGGAC 
AAACCAAAGC TGTACCGGCT CCAGCACAGC ATCACTGAGG TCGGCTCAGA CTGCACAGAG 
GACGGAGCCA TTCAGCTGCT CGGTCCGGGG ATTCTGCCCC ACCACTGCAA CCTCATGCAC 
AGTGAAGGCA TGGTGACGGT GACGCCGCAC GGCCCCGACG CCGACACCTT CGTCGACGGG 
CAACGGGTCA CTGAGACGAC GGTGCTGCGC TCGGGCTCCA CCCTCCAGTT TGGCCAAGCG 
CACGTCTTCA AGTTTGTGGA CCCGATGTTC GACCAGGGAG GGAAGAGAGA GCCGGCCGCC 
ATGATGAGGA GTCGACACAA GTCCGGCAGC GTACCCGAAA CCACCTTCGA CCTTCACGGA 
GACGTCCACA GCGGAGCCGC GCTGCCTACC AGCAAGAGCT CAGGAAAGCT GGAGATGGAG 
AGAGGAATGG TCAAACCGAT GATCAGAGGA GAGCAGCAGG ACGGCAGGTC TCAGGACATT 
TCAGCAGACA GAGCCGAGTT GACTCTGCCA GCCGGCATCG AGTTCAGAGA CAACTCTGAA 
GACACCTTCC TGTCGGCCAT CATCAACTAC ACCAACAGCT CCACGGTTCA CTTCAAACTC 
TCGCCCACGT ACGTCCTGTA CATGGCCTGC CGCTACATCC TGTCGCCCTC GTACCGGCCC 
GACATGTCGC CGTCCGAGAG GACGCACAAG GTCATCGCCA TCGTCAACAA GATGGTGAGC 
ATGATGGAGG GAGTCATCCA GGAGATTCCT GAAGGGGATC AGAAGCAGAA GAACATCGCG 
GGAGCGCTGG CTTTCTGGAT GGCCAACGCC TCCGAGCTGC TCAACTTCAT CAAGCAGGAC 
CGAGACCTGA ACCGCATCAC GCTGGACGCC CAGGACATCC TCGCCCATCT GGTTCAGATG 
GCTTTCAAGT ACCTGGTCCA CTGTCTGCAG GCTGATCTGA ATAACTACAT GCCGGCCTTC 
CTGGACGACC CCGAGGAACA CAACCCACAG AGACCCAAGA TCGAGGACGT CCTGCACACC 
CTCACGGGGG CGATGTCGTT ACTGCGGCGG TGTCGGGTCA ACGCCGCTCT CACCATCCAG 
CTCTTCTCGC AGCTCTTCCA CTTCATCAAC ATGTGGCTCT TCAACAAGCT GGTGACGGAC 
ACCGAGTCCG GGCTGTGCTG TCACTACTGG GGGGCCATCC TGCGGCAGCA GCTCAGCCAC 
ATCGAGGCCT GGGCGGAGAA ACAGGGGCTG GAGCTCGCCG CCGACTGCCA CCTCAGTCGA 
ATCGTGCAGG CCACCACCCT GCTGACCATG GACAAATACT CCATGCAGGA TGTGCCGAAC 
ATCCACAACA CCTGCTTCAA GCTCAACTCG CTGCAGCTCC ACGCCCTCAT GAACAACTAC 
CACTGCGCTC CGGACGAGCC GTACATCCCG CCGGAGCTGA TAGACCACGT GGTGGCAGTG 
GCAGAGAACA CAGCAGATGA GCTGGCGAGG AGCGACGGCA GAGAGGTTCA GCTGGAGGAG 
GATCCGGACC TCCAGCTGCC GTTCCTCCTT CCCGAGGACG GTTACTCCTG CGACGTGGTG 
CGCAGCCTCC CCAACGGCCT GCAGGACTTC CTGGAGCCGC TGCTGCAGAG AGGTTTCTGT 
CGGTTGGTAC CTCACCCTCG CTCTCCCGGT ACCTGGACGG TCCATTTTGA AGGAGCTGAC 
TGCGACAGCC ACTTCTCTGC TGCTGACAAC TCAGAGATGC CAATGAGGAA AGAGCCGGAG 
GTTGTCACGG TAACCCTGAA GAAGCACAAC GGCATGGGCC TCAGCATCGT GGCTGCCAAG 
GGTGCTGGTC AGGAGAAGCT GGGCATCTAC ATCAAGTCTG TCGTCAAAGG AGGAGCAGCT 
GATGTGGACG GGCGTCTGGC TGCAGGTGAC CAGCTGTTGA GCGTGGACGG GCGGAGTCTG 
GTCGGCCTAT CACAAGAGAG GGCAGCGGAG CTGATGACGA GGACGGGCTC AGTCGTGACT 
CTGGAGGTCG CCAAGCAGGG AGCCATTTAC CACGGACTGG CCACGCTCCT CAACCAGCCG 
AGCCCCATGA TGCCACGAGC GTCAGACAGA GGCCGCGACA AGAACGGGAA ACTCCGACCA 
AAGAGCGAAG GCTTCGAGTT GTACAACAGC TCGGTGCAGA ACGGCTCTCC GGAGAGCCCG 
CAGGCCGGCT GGGACTCTTA CCCCGAACCA AAGAAGATGA GCGGCGAAGA TCGACTCCTG 
AAGAACCGAG CCGACCACCG CTCCAGCCCC AACGTCGCCA ATCAGGGCCA GAGTCCTGCA 
GGCAAAGCAG TGTACCCCGG AGGACCTGGC ACCAAGATCA CCTCCGTCTC CACCGGCAAC 
CTGTGCGCAG ACGATGAGCC CTCCCCTCCA CGTCCAGAAG CTTACCCCAT CCCCACCCAG 
ACCTACCCCA GGGAGTACTT CACCATCCCG GCTTCCAAGA GCCAGGATCG GGTCGTTGGT 
CCTGGGCAGG GACAGCACTG GCAGGGCATG GAGGACAGAG AACGCCTCCC TGTGGTCGAC 
AACATCCACA ACAGCATGCA GCGGGTTAAC CACTCCCAGG AGGAGTTGTA CCCTCCACCC 
GGAGGCCTGA GGCCGGAGGA GCGCATGCAG CAGCACTACC AGCAGGACTA CCAGCACCGC 
GACATGGACT ACCAGCACAG AGAGCTCGAC TACCAGAGAG GAGGTCCACC AGGTGTCGGA 
GGTCCCGATC ACTGGACTCC ACAGCCTCAG GTGTCCTCCT CGCTGGAGTC GTCCACGTCC 
AGCCAGGAGC ACCTCAACTT CTCCGCCTCC TCCTCGTCCT CCAACAAAAC CCAGAACCAG 
AAGACCGGGC CGGGCCGGTG GAAGACTCCC AACGCCCCTC ACTCCGTGCC GCCACATTCA 
GCCCAGCCGC CCTCCCGCTC CGACCTGCCC CCTCCTCCGC CTCCACCGCC AGCTACTTAC 
CCCGAATCCT ACGACCCATA CGACCCCCAA CCGGACCTGC CGCTCCCGCC GCCTCCCTCC 
GCCGTCACTC CTGCGGTAGC TCAGCAGGCG GCCGACCGCA AGAAACGTGA GGAGCAGCAG 
CGCTGGTATG AGAAGGAGAA AGCTCGGCTG GAGGAGGAGA GGGAGAGGAA GAGGAGAGAG 
CAGGAGAGGA AGCTGGGTCA GATCCGGGCC AACCCCGTCA TGCCCGTCCA GCCTCACAAC 
AACGGAGGAT CCATGCCTCC GCCTCACCAC CAGCCGCCTC TGCCGTCTGC AGCCCCGCCT 
CAGCCCTACC TTCCCCCCGC TCAGCCCCAG CCTCCACCAT CCAGACCGGA GAAGCTGACC 
AGCCTGCCGC GGTCAGCCGT TCCTCCACCC GCCAGCGCCT CCAACCCTGC AGGCATGGAC 
ACGGTGATCA GGGAGCTGTT GCCACAGCAG CAGCCGCGGA CCATCGAGAG GCGAGACCTG 
CAGTACATCA CCATCAGCAA GGAGGAGCTC ACCTCCAGCG ACAGTCTGTC TCCAGACCCC 
TGGAAGCGAG ACGCCCGCGA GAAGGCCGAG AAGCAGCAGC AGCTCCACAT CGTCGACCTG 
CTGGACAAGG AGATCCAGGA GCTCCAGGCG AAGCCGGAGC GGACGGCGGA GGAGAGCGAC 
CGCCTCAGGA AGCTGATGCT GGAGTGGCAG TTCCAGAAAC GACTGCAGGA GTCCAAGCAG 
AGCGACGAAG ACGAGGAGGA GGAGGACGAC GAGGACGTGG ACACCATGAT GATCATGCAG 
AGGCTGGAGG CCGAGAAGAG AGCCAGGCAG CAGAATGCTG TCCCAGCTAT CTCTGTTCTA 
GACTTGTTGC AGGACGAGGA GCGGCGGCGT AAGCAGCAGC TGGAGGAGAT TCGTAAGCGG 
GAGGCGGAGG AGCGAGCAAA GCAGGAGGAG GAGAGGAGGT GGAGGGAGGA GGAGAGAGCC 
AAGCGAGAGG CCGAGGAGAA GAGGAGACAG GAGGAGGAGT ACTACACTCG CCTGGAGGCG 
GAGCGGCGCC GGCAGCACGA CGAGGCCGAG AGGAAGCTGC TGACGCCCGA CGACCCGGCC 
GGTCTGTATC GGCCCCCGCT GCCCCGGGAT TACCAAACCC CGCCCCCCCA ACAAACCTAT 
AAAACCCCCC CGCCCCCCCC ACAGAGAAAC ACCTCCTACC TCAAAACGCA GGTCACCTCC 
CCCGACACGC TCTACACCGC CAAGTTTGTC TCCTACGCGG GCGACGAGGA GGAAGACGAG 
GAGGCAGGGA GTGGCGGCGG CGGCGGCAGC AGCAGCCAAT CAGGTCAGGT GAAGCTGTCG 
GCGACCCGGA AGTCGTACGG CGACCTCCCG GCCTCCGCCT CGCCCAATCA CAGCAGGCCT 
CAGCCGCCGC CCACTGCGCG CAAGCCCCGC CCTGTTTCTG ACGGCATCTT CCTGTCTGGC 
TCGTTCCAGC CGCCATTCAA CAACAACTCC AACAGTACAG GTCCCCCCCT TCCTGCCAAA 
CCCAGCTCCA TCCCCCCGGG AGGCTTTAAA GGATTTAACT CGTACACTGG AAACTCAGCA 
GGAGTTGTAG GATCAGGAGA AGTCTACAAG GACCCGCGAG ACAAATGGAC GAAGAGTCAG 
GAGCAGGAGA ACTCGAACCC CAGCGGCCCT CCGGAGAATC TGACCTTCAA GGAGCGGCAG 
CGTCTCTTCT CTCAGGGGAA GGAGGTTTCC AACAAGGTCA AAGCTTCGCG CAAACTCATG 
GAGCTGGAGA ACGAGCTCAA CACCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afdn ( XM_027284629.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_027140430.1 Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OLa200711
Clone ID Related Accession (Same CDS sequence)	XM_027284629.1
Accession Version	XM_027284629.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	afadin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040021.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGCA GCAGGGAGGA GGAGAGGAGG AAACTGGCCG ACATCATCAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CGGCCCACAG AGGACCTGGA GTTCCATGGC 
GTGATGCGGT TCTACTTCCA GGACCGGGTC GCCGGTAACT TTGCCACCAA ATGCATCAGA 
GTGTCGAGCA CGGCGACAAC GCAGGACGTC ATCGAGACGT TAGCAGAGAA GTTCAGACCG 
GACATGAGGA TGCTCTCCTC GCCAAAATAC TCTTTGTACG AGGTCCACGT CAGTGGCGAA 
GAGCGTCAGC TGGATCTGGA CGAGAAGCCT CTGGTGGTTC AGCTGAACTG GAACAAAGAC 
GACAGAGAAG GACGCTTCGT CCTGAAGAAC GAGAACGACA TCCTCCCCAA GAAGAGTCAG 
AGTAACGGCC CGGAGAAGGA GAAGGACGGA GTGATCCAGA ACTTCAAGAG GACTTTGTCC 
AAGAAGGAGA AGAAGAAGGA GAAGAAGAGG GAGAAGGAGT TCGCCCGGAT CCCCGACGGA 
GACGAGCAGG CGCTCAGCCG AGAGGACGGG GAGAACAGTC GTCTGGCGGC GGAGGTTTAC 
AAGGACATGC CGGAGAACAG CTTCACCAGG ACGATCTCCA ACCCGGAGGT GGTGATGAAG 
AGGAGACGCC AGCAGAAGCT GGAGAAACGC ATGCAGGAGT TCATGAGCAG CGACGGGAGG 
CCCGACTCAG GGGGCACGTT GCGGATATAC GCCGACAGCC TGAAGCCGAA CATCCCGTAT 
AAAACCATCC TGCTGTCCAC GAGAGACACG GCTGACTTTG CGGTGGTGGA GGCGCTGGAG 
AAGTACGGGC TGGAGAAGGA GAACCCCCGA GAGTACTGCA TCGCCCGGCA AGACGATAAG 
TCCGGCAAGG AGGCGATCCT GGACGATACC GAGTGTCCAC TGCAGATCTT CAGGGACTGG 
CCTGCAGACA GAGGAGCGCT AGTGTTCCAG CTGAAGAAGA GACCCCCGGA CTACCAGGGC 
AGGAAGGGGA GGAAAGTGGA CGACAAGGGC CTGCGAGGGA AGGACGGCAG CAGCTCCCTG 
CCGCCCGAGA AACTGCCTTA CCTGGTGGAG CTGAGCCCAG GGCGAGGGAA TCACTATGCC 
TACTACGCCT ATCGGCACCA CGAAGACGGG TCTGACTCAC GGGACAAACC AAAGCTGTAC 
CGGCTCCAGC ACAGCATCAC TGAGGTCGGC TCAGACTGCA CAGAGGACGG AGCCATTCAG 
CTGCTCGGTC CGGGGATTCT GCCCCACCAC TGCAACCTCA TGCACAGTGA AGGCATGGTG 
ACGGTGACGC CGCACGGCCC CGACGCCGAC ACCTTCGTCG ACGGGCAACG GGTCACTGAG 
ACGACGGTGC TGCGCTCGGG CTCCACCCTC CAGTTTGGCC AAGCGCACGT CTTCAAGTTT 
GTGGACCCGA TGTTCGACCA GGGAGGGAAG AGAGAGCCGG CCGCCATGAT GAGGAGTCGA 
CACAAGTCCG GCAGCGTACC CGAAACCACC TTCGACCTTC ACGGAGACGT CCACAGCGGA 
GCCGCGCTGC CTACCAGCAA GAGCTCAGGA AAGCTGGAGA TGGAGAGAGG AATGGTCAAA 
CCGATGATCA GAGGAGAGCA GCAGGACGGC AGGTCTCAGG ACATTTCAGC AGACAGAGCC 
GAGTTGACTC TGCCAGCCGG CATCGAGTTC AGAGACAACT CTGAAGACAC CTTCCTGTCG 
GCCATCATCA ACTACACCAA CAGCTCCACG GTTCACTTCA AACTCTCGCC CACGTACGTC 
CTGTACATGG CCTGCCGCTA CATCCTGTCG CCCTCGTACC GGCCCGACAT GTCGCCGTCC 
GAGAGGACGC ACAAGGTCAT CGCCATCGTC AACAAGATGG TGAGCATGAT GGAGGGAGTC 
ATCCAGGAGA TTCCTGAAGG GGATCAGAAG CAGAAGAACA TCGCGGGAGC GCTGGCTTTC 
TGGATGGCCA ACGCCTCCGA GCTGCTCAAC TTCATCAAGC AGGACCGAGA CCTGAACCGC 
ATCACGCTGG ACGCCCAGGA CATCCTCGCC CATCTGGTTC AGATGGCTTT CAAGTACCTG 
GTCCACTGTC TGCAGGCTGA TCTGAATAAC TACATGCCGG CCTTCCTGGA CGACCCCGAG 
GAACACAACC CACAGAGACC CAAGATCGAG GACGTCCTGC ACACCCTCAC GGGGGCGATG 
TCGTTACTGC GGCGGTGTCG GGTCAACGCC GCTCTCACCA TCCAGCTCTT CTCGCAGCTC 
TTCCACTTCA TCAACATGTG GCTCTTCAAC AAGCTGGTGA CGGACACCGA GTCCGGGCTG 
TGCTGTCACT ACTGGGGGGC CATCCTGCGG CAGCAGCTCA GCCACATCGA GGCCTGGGCG 
GAGAAACAGG GGCTGGAGCT CGCCGCCGAC TGCCACCTCA GTCGAATCGT GCAGGCCACC 
ACCCTGCTGA CCATGGACAA ATACTCCATG CAGGATGTGC CGAACATCCA CAACACCTGC 
TTCAAGCTCA ACTCGCTGCA GCTCCACGCC CTCATGAACA ACTACCACTG CGCTCCGGAC 
GAGCCGTACA TCCCGCCGGA GCTGATAGAC CACGTGGTGG CAGTGGCAGA GAACACAGCA 
GATGAGCTGG CGAGGAGCGA CGGCAGAGAG GTTCAGCTGG AGGAGGATCC GGACCTCCAG 
CTGCCGTTCC TCCTTCCCGA GGACGGTTAC TCCTGCGACG TGGTGCGCAG CCTCCCCAAC 
GGCCTGCAGG ACTTCCTGGA GCCGCTGCTG CAGAGAGGTT TCTGTCGGTT GGTACCTCAC 
CCTCGCTCTC CCGGTACCTG GACGGTCCAT TTTGAAGGAG CTGACTGCGA CAGCCACTTC 
TCTGCTGCTG ACAACTCAGA GATGCCAATG AGGAAAGAGC CGGAGGTTGT CACGGTAACC 
CTGAAGAAGC ACAACGGCAT GGGCCTCAGC ATCGTGGCTG CCAAGGGTGC TGGTCAGGAG 
AAGCTGGGCA TCTACATCAA GTCTGTCGTC AAAGGAGGAG CAGCTGATGT GGACGGGCGT 
CTGGCTGCAG GTGACCAGCT GTTGAGCGTG GACGGGCGGA GTCTGGTCGG CCTATCACAA 
GAGAGGGCAG CGGAGCTGAT GACGAGGACG GGCTCAGTCG TGACTCTGGA GGTCGCCAAG 
CAGGGAGCCA TTTACCACGG ACTGGCCACG CTCCTCAACC AGCCGAGCCC CATGATGCCA 
CGAGCGTCAG ACAGAGGCCG CGACAAGAAC GGGAAACTCC GACCAAAGAG CGAAGGCTTC 
GAGTTGTACA ACAGCTCGGT GCAGAACGGC TCTCCGGAGA GCCCGCAGGC CGGCTGGGAC 
TCTTACCCCG AACCAAAGAA GATGAGCGGC GAAGATCGAC TCCTGAAGAA CCGAGCCGAC 
CACCGCTCCA GCCCCAACGT CGCCAATCAG GGCCAGAGTC CTGCAGGCAA AGCAGTGTAC 
CCCGGAGGAC CTGGCACCAA GATCACCTCC GTCTCCACCG GCAACCTGTG CGCAGACGAT 
GAGCCCTCCC CTCCACGTCC AGAAGCTTAC CCCATCCCCA CCCAGACCTA CCCCAGGGAG 
TACTTCACCA TCCCGGCTTC CAAGAGCCAG GATCGGGTCG TTGGTCCTGG GCAGGGACAG 
CACTGGCAGG GCATGGAGGA CAGAGAACGC CTCCCTGTGG TCGACAACAT CCACAACAGC 
ATGCAGCGGG TTAACCACTC CCAGGAGGAG TTGTACCCTC CACCCGGAGG CCTGAGGCCG 
GAGGAGCGCA TGCAGCAGCA CTACCAGCAG GACTACCAGC ACCGCGACAT GGACTACCAG 
CACAGAGAGC TCGACTACCA GAGAGGAGGT CCACCAGGTG TCGGAGGTCC CGATCACTGG 
ACTCCACAGC CTCAGGTGTC CTCCTCGCTG GAGTCGTCCA CGTCCAGCCA GGAGCACCTC 
AACTTCTCCG CCTCCTCCTC GTCCTCCAAC AAAACCCAGA ACCAGAAGAC CGGGCCGGGC 
CGGTGGAAGA CTCCCAACGC CCCTCACTCC GTGCCGCCAC ATTCAGCCCA GCCGCCCTCC 
CGCTCCGACC TGCCCCCTCC TCCGCCTCCA CCGCCAGCTA CTTACCCCGA ATCCTACGAC 
CCATACGACC CCCAACCGGA CCTGCCGCTC CCGCCGCCTC CCTCCGCCGT CACTCCTGCG 
GTAGCTCAGC AGGCGGCCGA CCGCAAGAAA CGTGAGGAGC AGCAGCGCTG GTATGAGAAG 
GAGAAAGCTC GGCTGGAGGA GGAGAGGGAG AGGAAGAGGA GAGAGCAGGA GAGGAAGCTG 
GGTCAGATCC GGGCCAACCC CGTCATGCCC GTCCAGCCTC ACAACAACGG AGGATCCATG 
CCTCCGCCTC ACCACCAGCC GCCTCTGCCG TCTGCAGCCC CGCCTCAGCC CTACCTTCCC 
CCCGCTCAGC CCCAGCCTCC ACCATCCAGA CCGGAGAAGC TGACCAGCCT GCCGCGGTCA 
GCCGTTCCTC CACCCGCCAG CGCCTCCAAC CCTGCAGGCA TGGACACGGT GATCAGGGAG 
CTGTTGCCAC AGCAGCAGCC GCGGACCATC GAGAGGCGAG ACCTGCAGTA CATCACCATC 
AGCAAGGAGG AGCTCACCTC CAGCGACAGT CTGTCTCCAG ACCCCTGGAA GCGAGACGCC 
CGCGAGAAGG CCGAGAAGCA GCAGCAGCTC CACATCGTCG ACCTGCTGGA CAAGGAGATC 
CAGGAGCTCC AGGCGAAGCC GGAGCGGACG GCGGAGGAGA GCGACCGCCT CAGGAAGCTG 
ATGCTGGAGT GGCAGTTCCA GAAACGACTG CAGGAGTCCA AGCAGAGCGA CGAAGACGAG 
GAGGAGGAGG ACGACGAGGA CGTGGACACC ATGATGATCA TGCAGAGGCT GGAGGCCGAG 
AAGAGAGCCA GGCAGCAGAA TGCTGTCCCA GCTATCTCTG TTCTAGACTT GTTGCAGGAC 
GAGGAGCGGC GGCGTAAGCA GCAGCTGGAG GAGATTCGTA AGCGGGAGGC GGAGGAGCGA 
GCAAAGCAGG AGGAGGAGAG GAGGTGGAGG GAGGAGGAGA GAGCCAAGCG AGAGGCCGAG 
GAGAAGAGGA GACAGGAGGA GGAGTACTAC ACTCGCCTGG AGGCGGAGCG GCGCCGGCAG 
CACGACGAGG CCGAGAGGAA GCTGCTGACG CCCGACGACC CGGCCGGTCT GTATCGGCCC 
CCGCTGCCCC GGGATTACCA AACCCCGCCC CCCCAACAAA CCTATAAAAC CCCCCCGCCC 
CCCCCACAGA GAAACACCTC CTACCTCAAA ACGCAGGTCA CCTCCCCCGA CACGCTCTAC 
ACCGCCAAGT TTGTCTCCTA CGCGGGCGAC GAGGAGGAAG ACGAGGAGGC AGGGAGTGGC 
GGCGGCGGCG GCAGCAGCAG CCAATCAGGA TTTAACTCGT ACACTGGAAA CTCAGCAGGA 
GTTGTAGGAT CAGGAGAAGT CTACAAGGAC CCGCGAGACA AATGGACGAA GAGTCAGGAG 
CAGGAGAACT CGAACCCCAG CGGCCCTCCG GAGAATCTGA CCTTCAAGGA GCGGCAGCGT 
CTCTTCTCTC AGGGGAAGGA GGTTTCCAAC AAGGTCAAAG CTTCGCGCAA ACTCATGGAG 
CTGGAGAACG AGCTCAACAC CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027140430.1
CDS	233..5899
Translation	MSGSREEERRKLADIINHWNANRLDLFEISRPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERQLDLDEKPLVVQLNWNKDDREGRFVLKNENDILPKKSQSNGPEKEKDGVIQNFKRTLSKKEKKKEKKREKEFARIPDGDEQALSREDGENSRLAAEVYKDMPENSFTRTISNPEVVMKRRRQQKLEKRMQEFMSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTRDTADFAVVEALEKYGLEKENPREYCIARQDDKSGKEAILDDTECPLQIFRDWPADRGALVFQLKKRPPDYQGRKGRKVDDKGLRGKDGSSSLPPEKLPYLVELSPGRGNHYAYYAYRHHEDGSDSRDKPKLYRLQHSITEVGSDCTEDGAIQLLGPGILPHHCNLMHSEGMVTVTPHGPDADTFVDGQRVTETTVLRSGSTLQFGQAHVFKFVDPMFDQGGKREPAAMMRSRHKSGSVPETTFDLHGDVHSGAALPTSKSSGKLEMERGMVKPMIRGEQQDGRSQDISADRAELTLPAGIEFRDNSEDTFLSAIINYTNSSTVHFKLSPTYVLYMACRYILSPSYRPDMSPSERTHKVIAIVNKMVSMMEGVIQEIPEGDQKQKNIAGALAFWMANASELLNFIKQDRDLNRITLDAQDILAHLVQMAFKYLVHCLQADLNNYMPAFLDDPEEHNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVTDTESGLCCHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVPNIHNTCFKLNSLQLHALMNNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRSLPNGLQDFLEPLLQRGFCRLVPHPRSPGTWTVHFEGADCDSHFSAADNSEMPMRKEPEVVTVTLKKHNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRDKNGKLRPKSEGFELYNSSVQNGSPESPQAGWDSYPEPKKMSGEDRLLKNRADHRSSPNVANQGQSPAGKAVYPGGPGTKITSVSTGNLCADDEPSPPRPEAYPIPTQTYPREYFTIPASKSQDRVVGPGQGQHWQGMEDRERLPVVDNIHNSMQRVNHSQEELYPPPGGLRPEERMQQHYQQDYQHRDMDYQHRELDYQRGGPPGVGGPDHWTPQPQVSSSLESSTSSQEHLNFSASSSSSNKTQNQKTGPGRWKTPNAPHSVPPHSAQPPSRSDLPPPPPPPPATYPESYDPYDPQPDLPLPPPPSAVTPAVAQQAADRKKREEQQRWYEKEKARLEEERERKRREQERKLGQIRANPVMPVQPHNNGGSMPPPHHQPPLPSAAPPQPYLPPAQPQPPPSRPEKLTSLPRSAVPPPASASNPAGMDTVIRELLPQQQPRTIERRDLQYITISKEELTSSDSLSPDPWKRDAREKAEKQQQLHIVDLLDKEIQELQAKPERTAEESDRLRKLMLEWQFQKRLQESKQSDEDEEEEDDEDVDTMMIMQRLEAEKRARQQNAVPAISVLDLLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERAKREAEEKRRQEEEYYTRLEAERRRQHDEAERKLLTPDDPAGLYRPPLPRDYQTPPPQQTYKTPPPPPQRNTSYLKTQVTSPDTLYTAKFVSYAGDEEEDEEAGSGGGGGSSSQSGFNSYTGNSAGVVGSGEVYKDPRDKWTKSQEQENSNPSGPPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNTK

Target ORF information:

RefSeq Version	XM_027284629.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027284629.1

ORF Insert Sequence:

ATGTCGGGCA GCAGGGAGGA GGAGAGGAGG AAACTGGCCG ACATCATCAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CGGCCCACAG AGGACCTGGA GTTCCATGGC 
GTGATGCGGT TCTACTTCCA GGACCGGGTC GCCGGTAACT TTGCCACCAA ATGCATCAGA 
GTGTCGAGCA CGGCGACAAC GCAGGACGTC ATCGAGACGT TAGCAGAGAA GTTCAGACCG 
GACATGAGGA TGCTCTCCTC GCCAAAATAC TCTTTGTACG AGGTCCACGT CAGTGGCGAA 
GAGCGTCAGC TGGATCTGGA CGAGAAGCCT CTGGTGGTTC AGCTGAACTG GAACAAAGAC 
GACAGAGAAG GACGCTTCGT CCTGAAGAAC GAGAACGACA TCCTCCCCAA GAAGAGTCAG 
AGTAACGGCC CGGAGAAGGA GAAGGACGGA GTGATCCAGA ACTTCAAGAG GACTTTGTCC 
AAGAAGGAGA AGAAGAAGGA GAAGAAGAGG GAGAAGGAGT TCGCCCGGAT CCCCGACGGA 
GACGAGCAGG CGCTCAGCCG AGAGGACGGG GAGAACAGTC GTCTGGCGGC GGAGGTTTAC 
AAGGACATGC CGGAGAACAG CTTCACCAGG ACGATCTCCA ACCCGGAGGT GGTGATGAAG 
AGGAGACGCC AGCAGAAGCT GGAGAAACGC ATGCAGGAGT TCATGAGCAG CGACGGGAGG 
CCCGACTCAG GGGGCACGTT GCGGATATAC GCCGACAGCC TGAAGCCGAA CATCCCGTAT 
AAAACCATCC TGCTGTCCAC GAGAGACACG GCTGACTTTG CGGTGGTGGA GGCGCTGGAG 
AAGTACGGGC TGGAGAAGGA GAACCCCCGA GAGTACTGCA TCGCCCGGCA AGACGATAAG 
TCCGGCAAGG AGGCGATCCT GGACGATACC GAGTGTCCAC TGCAGATCTT CAGGGACTGG 
CCTGCAGACA GAGGAGCGCT AGTGTTCCAG CTGAAGAAGA GACCCCCGGA CTACCAGGGC 
AGGAAGGGGA GGAAAGTGGA CGACAAGGGC CTGCGAGGGA AGGACGGCAG CAGCTCCCTG 
CCGCCCGAGA AACTGCCTTA CCTGGTGGAG CTGAGCCCAG GGCGAGGGAA TCACTATGCC 
TACTACGCCT ATCGGCACCA CGAAGACGGG TCTGACTCAC GGGACAAACC AAAGCTGTAC 
CGGCTCCAGC ACAGCATCAC TGAGGTCGGC TCAGACTGCA CAGAGGACGG AGCCATTCAG 
CTGCTCGGTC CGGGGATTCT GCCCCACCAC TGCAACCTCA TGCACAGTGA AGGCATGGTG 
ACGGTGACGC CGCACGGCCC CGACGCCGAC ACCTTCGTCG ACGGGCAACG GGTCACTGAG 
ACGACGGTGC TGCGCTCGGG CTCCACCCTC CAGTTTGGCC AAGCGCACGT CTTCAAGTTT 
GTGGACCCGA TGTTCGACCA GGGAGGGAAG AGAGAGCCGG CCGCCATGAT GAGGAGTCGA 
CACAAGTCCG GCAGCGTACC CGAAACCACC TTCGACCTTC ACGGAGACGT CCACAGCGGA 
GCCGCGCTGC CTACCAGCAA GAGCTCAGGA AAGCTGGAGA TGGAGAGAGG AATGGTCAAA 
CCGATGATCA GAGGAGAGCA GCAGGACGGC AGGTCTCAGG ACATTTCAGC AGACAGAGCC 
GAGTTGACTC TGCCAGCCGG CATCGAGTTC AGAGACAACT CTGAAGACAC CTTCCTGTCG 
GCCATCATCA ACTACACCAA CAGCTCCACG GTTCACTTCA AACTCTCGCC CACGTACGTC 
CTGTACATGG CCTGCCGCTA CATCCTGTCG CCCTCGTACC GGCCCGACAT GTCGCCGTCC 
GAGAGGACGC ACAAGGTCAT CGCCATCGTC AACAAGATGG TGAGCATGAT GGAGGGAGTC 
ATCCAGGAGA TTCCTGAAGG GGATCAGAAG CAGAAGAACA TCGCGGGAGC GCTGGCTTTC 
TGGATGGCCA ACGCCTCCGA GCTGCTCAAC TTCATCAAGC AGGACCGAGA CCTGAACCGC 
ATCACGCTGG ACGCCCAGGA CATCCTCGCC CATCTGGTTC AGATGGCTTT CAAGTACCTG 
GTCCACTGTC TGCAGGCTGA TCTGAATAAC TACATGCCGG CCTTCCTGGA CGACCCCGAG 
GAACACAACC CACAGAGACC CAAGATCGAG GACGTCCTGC ACACCCTCAC GGGGGCGATG 
TCGTTACTGC GGCGGTGTCG GGTCAACGCC GCTCTCACCA TCCAGCTCTT CTCGCAGCTC 
TTCCACTTCA TCAACATGTG GCTCTTCAAC AAGCTGGTGA CGGACACCGA GTCCGGGCTG 
TGCTGTCACT ACTGGGGGGC CATCCTGCGG CAGCAGCTCA GCCACATCGA GGCCTGGGCG 
GAGAAACAGG GGCTGGAGCT CGCCGCCGAC TGCCACCTCA GTCGAATCGT GCAGGCCACC 
ACCCTGCTGA CCATGGACAA ATACTCCATG CAGGATGTGC CGAACATCCA CAACACCTGC 
TTCAAGCTCA ACTCGCTGCA GCTCCACGCC CTCATGAACA ACTACCACTG CGCTCCGGAC 
GAGCCGTACA TCCCGCCGGA GCTGATAGAC CACGTGGTGG CAGTGGCAGA GAACACAGCA 
GATGAGCTGG CGAGGAGCGA CGGCAGAGAG GTTCAGCTGG AGGAGGATCC GGACCTCCAG 
CTGCCGTTCC TCCTTCCCGA GGACGGTTAC TCCTGCGACG TGGTGCGCAG CCTCCCCAAC 
GGCCTGCAGG ACTTCCTGGA GCCGCTGCTG CAGAGAGGTT TCTGTCGGTT GGTACCTCAC 
CCTCGCTCTC CCGGTACCTG GACGGTCCAT TTTGAAGGAG CTGACTGCGA CAGCCACTTC 
TCTGCTGCTG ACAACTCAGA GATGCCAATG AGGAAAGAGC CGGAGGTTGT CACGGTAACC 
CTGAAGAAGC ACAACGGCAT GGGCCTCAGC ATCGTGGCTG CCAAGGGTGC TGGTCAGGAG 
AAGCTGGGCA TCTACATCAA GTCTGTCGTC AAAGGAGGAG CAGCTGATGT GGACGGGCGT 
CTGGCTGCAG GTGACCAGCT GTTGAGCGTG GACGGGCGGA GTCTGGTCGG CCTATCACAA 
GAGAGGGCAG CGGAGCTGAT GACGAGGACG GGCTCAGTCG TGACTCTGGA GGTCGCCAAG 
CAGGGAGCCA TTTACCACGG ACTGGCCACG CTCCTCAACC AGCCGAGCCC CATGATGCCA 
CGAGCGTCAG ACAGAGGCCG CGACAAGAAC GGGAAACTCC GACCAAAGAG CGAAGGCTTC 
GAGTTGTACA ACAGCTCGGT GCAGAACGGC TCTCCGGAGA GCCCGCAGGC CGGCTGGGAC 
TCTTACCCCG AACCAAAGAA GATGAGCGGC GAAGATCGAC TCCTGAAGAA CCGAGCCGAC 
CACCGCTCCA GCCCCAACGT CGCCAATCAG GGCCAGAGTC CTGCAGGCAA AGCAGTGTAC 
CCCGGAGGAC CTGGCACCAA GATCACCTCC GTCTCCACCG GCAACCTGTG CGCAGACGAT 
GAGCCCTCCC CTCCACGTCC AGAAGCTTAC CCCATCCCCA CCCAGACCTA CCCCAGGGAG 
TACTTCACCA TCCCGGCTTC CAAGAGCCAG GATCGGGTCG TTGGTCCTGG GCAGGGACAG 
CACTGGCAGG GCATGGAGGA CAGAGAACGC CTCCCTGTGG TCGACAACAT CCACAACAGC 
ATGCAGCGGG TTAACCACTC CCAGGAGGAG TTGTACCCTC CACCCGGAGG CCTGAGGCCG 
GAGGAGCGCA TGCAGCAGCA CTACCAGCAG GACTACCAGC ACCGCGACAT GGACTACCAG 
CACAGAGAGC TCGACTACCA GAGAGGAGGT CCACCAGGTG TCGGAGGTCC CGATCACTGG 
ACTCCACAGC CTCAGGTGTC CTCCTCGCTG GAGTCGTCCA CGTCCAGCCA GGAGCACCTC 
AACTTCTCCG CCTCCTCCTC GTCCTCCAAC AAAACCCAGA ACCAGAAGAC CGGGCCGGGC 
CGGTGGAAGA CTCCCAACGC CCCTCACTCC GTGCCGCCAC ATTCAGCCCA GCCGCCCTCC 
CGCTCCGACC TGCCCCCTCC TCCGCCTCCA CCGCCAGCTA CTTACCCCGA ATCCTACGAC 
CCATACGACC CCCAACCGGA CCTGCCGCTC CCGCCGCCTC CCTCCGCCGT CACTCCTGCG 
GTAGCTCAGC AGGCGGCCGA CCGCAAGAAA CGTGAGGAGC AGCAGCGCTG GTATGAGAAG 
GAGAAAGCTC GGCTGGAGGA GGAGAGGGAG AGGAAGAGGA GAGAGCAGGA GAGGAAGCTG 
GGTCAGATCC GGGCCAACCC CGTCATGCCC GTCCAGCCTC ACAACAACGG AGGATCCATG 
CCTCCGCCTC ACCACCAGCC GCCTCTGCCG TCTGCAGCCC CGCCTCAGCC CTACCTTCCC 
CCCGCTCAGC CCCAGCCTCC ACCATCCAGA CCGGAGAAGC TGACCAGCCT GCCGCGGTCA 
GCCGTTCCTC CACCCGCCAG CGCCTCCAAC CCTGCAGGCA TGGACACGGT GATCAGGGAG 
CTGTTGCCAC AGCAGCAGCC GCGGACCATC GAGAGGCGAG ACCTGCAGTA CATCACCATC 
AGCAAGGAGG AGCTCACCTC CAGCGACAGT CTGTCTCCAG ACCCCTGGAA GCGAGACGCC 
CGCGAGAAGG CCGAGAAGCA GCAGCAGCTC CACATCGTCG ACCTGCTGGA CAAGGAGATC 
CAGGAGCTCC AGGCGAAGCC GGAGCGGACG GCGGAGGAGA GCGACCGCCT CAGGAAGCTG 
ATGCTGGAGT GGCAGTTCCA GAAACGACTG CAGGAGTCCA AGCAGAGCGA CGAAGACGAG 
GAGGAGGAGG ACGACGAGGA CGTGGACACC ATGATGATCA TGCAGAGGCT GGAGGCCGAG 
AAGAGAGCCA GGCAGCAGAA TGCTGTCCCA GCTATCTCTG TTCTAGACTT GTTGCAGGAC 
GAGGAGCGGC GGCGTAAGCA GCAGCTGGAG GAGATTCGTA AGCGGGAGGC GGAGGAGCGA 
GCAAAGCAGG AGGAGGAGAG GAGGTGGAGG GAGGAGGAGA GAGCCAAGCG AGAGGCCGAG 
GAGAAGAGGA GACAGGAGGA GGAGTACTAC ACTCGCCTGG AGGCGGAGCG GCGCCGGCAG 
CACGACGAGG CCGAGAGGAA GCTGCTGACG CCCGACGACC CGGCCGGTCT GTATCGGCCC 
CCGCTGCCCC GGGATTACCA AACCCCGCCC CCCCAACAAA CCTATAAAAC CCCCCCGCCC 
CCCCCACAGA GAAACACCTC CTACCTCAAA ACGCAGGTCA CCTCCCCCGA CACGCTCTAC 
ACCGCCAAGT TTGTCTCCTA CGCGGGCGAC GAGGAGGAAG ACGAGGAGGC AGGGAGTGGC 
GGCGGCGGCG GCAGCAGCAG CCAATCAGGA TTTAACTCGT ACACTGGAAA CTCAGCAGGA 
GTTGTAGGAT CAGGAGAAGT CTACAAGGAC CCGCGAGACA AATGGACGAA GAGTCAGGAG 
CAGGAGAACT CGAACCCCAG CGGCCCTCCG GAGAATCTGA CCTTCAAGGA GCGGCAGCGT 
CTCTTCTCTC AGGGGAAGGA GGTTTCCAAC AAGGTCAAAG CTTCGCGCAA ACTCATGGAG 
CTGGAGAACG AGCTCAACAC CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afdn ( XM_027284630.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_027140431.1 Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X10, mRNA.

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CloneID	OLa200712
Clone ID Related Accession (Same CDS sequence)	XM_027284630.1
Accession Version	XM_027284630.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5445bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	afadin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040021.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGCA GCAGGGAGGA GGAGAGGAGG AAACTGGCCG ACATCATCAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CGGCCCACAG AGGACCTGGA GTTCCATGGC 
GTGATGCGGT TCTACTTCCA GGACCGGGTC GCCGGTAACT TTGCCACCAA ATGCATCAGA 
GTGTCGAGCA CGGCGACAAC GCAGGACGTC ATCGAGACGT TAGCAGAGAA GTTCAGACCG 
GACATGAGGA TGCTCTCCTC GCCAAAATAC TCTTTGTACG AGGTCCACGT CAGTGGCGAA 
GAGCGTCAGC TGGATCTGGA CGAGAAGCCT CTGGTGGTTC AGCTGAACTG GAACAAAGAC 
GACAGAGAAG GACGCTTCGT CCTGAAGAAC GAGAACGACA TCCTCCCCAA GAAGAGTCAG 
AGTAACGGCC CGGAGAAGGA GAAGGACGGA GTGATCCAGA ACTTCAAGAG GACTTTGTCC 
AAGAAGGAGA AGAAGAAGGA GAAGAAGAGG GAGAAGGAGT TCGCCCGGAT CCCCGACGGA 
GACGAGCAGG CGCTCAGCCG AGAGGACGGG GAGAACAGTC GTCTGGCGGC GGAGGTTTAC 
AAGGACATGC CGGAGAACAG CTTCACCAGG ACGATCTCCA ACCCGGAGGT GGTGATGAAG 
AGGAGACGCC AGCAGAAGCT GGAGAAACGC ATGCAGGAGT TCATGAGCAG CGACGGGAGG 
CCCGACTCAG GGGGCACGTT GCGGATATAC GCCGACAGCC TGAAGCCGAA CATCCCGTAT 
AAAACCATCC TGCTGTCCAC GAGAGACACG GCTGACTTTG CGGTGGTGGA GGCGCTGGAG 
AAGTACGGGC TGGAGAAGGA GAACCCCCGA GAGTACTGCA TCGCCCGGCA AGACGATAAG 
TCCGGCAAGG AGGCGATCCT GGACGATACC GAGTGTCCAC TGCAGATCTT CAGGGACTGG 
CCTGCAGACA GAGGAGCGCT AGTGTTCCAG CTGAAGAAGA GACCCCCGGA CTACCAGGGC 
AGGAAGGGGA GGAAAGTGGA CGACAAGGGC CTGCGAGGGA AGGACGGCAG CAGCTCCCTG 
CCGCCCGAGA AACTGCCTTA CCTGGTGGAG CTGAGCCCAG GGCGAGGGAA TCACTATGCC 
TACTACGCCT ATCGGCACCA CGAAGACGGG TCTGACTCAC GGGACAAACC AAAGCTGTAC 
CGGCTCCAGC ACAGCATCAC TGAGGTCGGC TCAGACTGCA CAGAGGACGG AGCCATTCAG 
CTGCTCGGTC CGGGGATTCT GCCCCACCAC TGCAACCTCA TGCACAGTGA AGGCATGGTG 
ACGGTGACGC CGCACGGCCC CGACGCCGAC ACCTTCGTCG ACGGGCAACG GGTCACTGAG 
ACGACGGTGC TGCGCTCGGG CTCCACCCTC CAGTTTGGCC AAGCGCACGT CTTCAAGTTT 
GTGGACCCGA TGTTCGACCA GGGAGGGAAG AGAGAGCCGG CCGCCATGAT GAGGAGTCGA 
CACAAGTCCG GCAGCGTACC CGAAACCACC TTCGACCTTC ACGGAGACGT CCACAGCGGA 
GCCGCGCTGC CTACCAGCAA GAGCTCAGGA AAGCTGGAGA TGGAGAGAGG AATGGTCAAA 
CCGATGATCA GAGGAGAGCA GCAGGACGGC AGGTCTCAGG ACATTTCAGC AGACAGAGCC 
GAGTTGACTC TGCCAGCCGG CATCGAGTTC AGAGACAACT CTGAAGACAC CTTCCTGTCG 
GCCATCATCA ACTACACCAA CAGCTCCACG GTTCACTTCA AACTCTCGCC CACGTACGTC 
CTGTACATGG CCTGCCGCTA CATCCTGTCG CCCTCGTACC GGCCCGACAT GTCGCCGTCC 
GAGAGGACGC ACAAGGTCAT CGCCATCGTC AACAAGATGG TGAGCATGAT GGAGGGAGTC 
ATCCAGGAGA TTCCTGAAGG GGATCAGAAG CAGAAGAACA TCGCGGGAGC GCTGGCTTTC 
TGGATGGCCA ACGCCTCCGA GCTGCTCAAC TTCATCAAGC AGGACCGAGA CCTGAACCGC 
ATCACGCTGG ACGCCCAGGA CATCCTCGCC CATCTGGTTC AGATGGCTTT CAAGTACCTG 
GTCCACTGTC TGCAGGCTGA TCTGAATAAC TACATGCCGG CCTTCCTGGA CGACCCCGAG 
GAACACAACC CACAGAGACC CAAGATCGAG GACGTCCTGC ACACCCTCAC GGGGGCGATG 
TCGTTACTGC GGCGGTGTCG GGTCAACGCC GCTCTCACCA TCCAGCTCTT CTCGCAGCTC 
TTCCACTTCA TCAACATGTG GCTCTTCAAC AAGCTGGTGA CGGACACCGA GTCCGGGCTG 
TGCTGTCACT ACTGGGGGGC CATCCTGCGG CAGCAGCTCA GCCACATCGA GGCCTGGGCG 
GAGAAACAGG GGCTGGAGCT CGCCGCCGAC TGCCACCTCA GTCGAATCGT GCAGGCCACC 
ACCCTGCTGA CCATGGACAA ATACTCCATG CAGGATGTGC CGAACATCCA CAACACCTGC 
TTCAAGCTCA ACTCGCTGCA GCTCCACGCC CTCATGAACA ACTACCACTG CGCTCCGGAC 
GAGCCGTACA TCCCGCCGGA GCTGATAGAC CACGTGGTGG CAGTGGCAGA GAACACAGCA 
GATGAGCTGG CGAGGAGCGA CGGCAGAGAG GTTCAGCTGG AGGAGGATCC GGACCTCCAG 
CTGCCGTTCC TCCTTCCCGA GGACGGTTAC TCCTGCGACG TGGTGCGCAG CCTCCCCAAC 
GGCCTGCAGG ACTTCCTGGA GCCGCTGCTG CAGAGAGGTT TCTGTCGGTT GGTACCTCAC 
CCTCGCTCTC CCGGTACCTG GACGGTCCAT TTTGAAGGAG CTGACTGCGA CAGCCACTTC 
TCTGCTGCTG ACAACTCAGA GATGCCAATG AGGAAAGAGC CGGAGGTTGT CACGGTAACC 
CTGAAGAAGC ACAACGGCAT GGGCCTCAGC ATCGTGGCTG CCAAGGGTGC TGGTCAGGAG 
AAGCTGGGCA TCTACATCAA GTCTGTCGTC AAAGGAGGAG CAGCTGATGT GGACGGGCGT 
CTGGCTGCAG GTGACCAGCT GTTGAGCGTG GACGGGCGGA GTCTGGTCGG CCTATCACAA 
GAGAGGGCAG CGGAGCTGAT GACGAGGACG GGCTCAGTCG TGACTCTGGA GGTCGCCAAG 
CAGGGAGCCA TTTACCACGG ACTGGCCACG CTCCTCAACC AGCCGAGCCC CATGATGCCA 
CGAGCGTCAG ACAGAGGCCG CGACAAGAAC GGGAAACTCC GACCAAAGAG CGAAGGCTTC 
GAGTTGTACA ACAGCTCGGT GCAGAACGGC TCTCCGGAGA GCCCGCAGGC CGGCTGGGAC 
TCTTACCCCG AACCAAAGAA GATGAGCGGC GAAGATCGAC TCCTGAAGAA CCGAGCCGAC 
CACCGCTCCA GCCCCAACGT CGCCAATCAG GGCCAGAGTC CTGCAGGCAA AGCAGTGTAC 
CCCGGAGGAC CTGGCACCAA GATCACCTCC GTCTCCACCG GCAACCTGTG CGCAGACGAT 
GAGCCCTCCC CTCCACGTCC AGAAGCTTAC CCCATCCCCA CCCAGACCTA CCCCAGGGAG 
TACTTCACCA TCCCGGCTTC CAAGAGCCAG GATCGGGTCG TTGGTCCTGG GCAGGGACAG 
CACTGGCAGG GCATGGAGGA CAGAGAACGC CTCCCTGTGG TCGACAACAT CCACAACAGC 
ATGCAGCGGG TTAACCACTC CCAGGAGGAG TTGTACCCTC CACCCGGAGG CCTGAGGCCG 
GAGGAGCGCA TGCAGCAGCA CTACCAGCAG GACTACCAGC ACCGCGACAT GGACTACCAG 
CACAGAGAGC TCGACTACCA GAGAGGAGGT CCACCAGGTG TCGGAGGTCC CGATCACTGG 
ACTCCACAGC CTCAGGTGTC CTCCTCGCTG GAGTCGTCCA CGTCCAGCCA GGAGCACCTC 
AACTTCTCCG CCTCCTCCTC GTCCTCCAAC AAAACCCAGA ACCAGAAGAC CGGGCCGGGC 
CGGTGGAAGA CTCCCAACGC CCCTCACTCC GTGCCGCCAC ATTCAGCCCA GCCGCCCTCC 
CGCTCCGACC TGCCCCCTCC TCCGCCTCCA CCGCCAGCTA CTTACCCCGA ATCCTACGAC 
CCATACGACC CCCAACCGGA CCTGCCGCTC CCGCCGCCTC CCTCCGCCGT CACTCCTGCG 
GTAGCTCAGC AGGCGGCCGA CCGCAAGAAA CGTGAGGAGC AGCAGCGCTG GTATGAGAAG 
GAGAAAGCTC GGCTGGAGGA GGAGAGGGAG AGGAAGAGGA GAGAGCAGGA GAGGAAGCTG 
GGTCAGATCC GGGCCAACCC CGTCATGCCC GTCCAGCCTC ACAACAACGG AGGATCCATG 
CCTCCGCCTC ACCACCAGCC GCCTCTGCCG TCTGCAGCCC CGCCTCAGCC CTACCTTCCC 
CCCGCTCAGC CCCAGCCTCC ACCATCCAGA CCGGAGAAGC TGACCAGCCT GCCGCGGTCA 
GCCGTTCCTC CACCCGCCAG CGCCTCCAAC CCTGCAGGCA TGGACACGGT GATCAGGGAG 
CTGTTGCCAC AGCAGCAGCC GCGGACCATC GAGAGGCGAG ACCTGCAGTA CATCACCATC 
AGCAAGGAGG AGCTCACCTC CAGCGACAGT CTGTCTCCAG ACCCCTGGAA GCGAGACGCC 
CGCGAGAAGG CCGAGAAGCA GCAGCAGCTC CACATCGTCG ACCTGCTGGA CAAGGAGATC 
CAGGAGCTCC AGGCGAAGCC GGAGCGGACG GCGGAGGAGA GCGACCGCCT CAGGAAGCTG 
ATGCTGGAGT GGCAGTTCCA GAAACGACTG CAGGAGTCCA AGCAGAGCGA CGAAGACGAG 
GAGGAGGAGG ACGACGAGGA CGTGGACACC ATGATGATCA TGCAGAGGCT GGAGGCCGAG 
AAGAGAGCCA GGCAGCAGAA TGCTGTCCCA GCTATCTCTG TTCTAGACTT GTTGCAGGAC 
GAGGAGCGGC GGCGTAAGCA GCAGCTGGAG GAGATTCGTA AGCGGGAGGC GGAGGAGCGA 
GCAAAGCAGG AGGAGGAGAG GAGGTGGAGG GAGGAGGAGA GAGCCAAGCG AGAGGCCGAG 
GAGAAGAGGA GACAGGAGGA GGAGTACTAC ACTCGCCTGG AGGCGGAGCG GCGCCGGCAG 
CACGACGAGG CCGAGAGGAA GCTGCTGACG CCCGACGACC CGGCCGGATT TAACTCGTAC 
ACTGGAAACT CAGCAGGAGT TGTAGGATCA GGAGAAGTCT ACAAGGACCC GCGAGACAAA 
TGGACGAAGA GTCAGGAGCA GGAGAACTCG AACCCCAGCG GCCCTCCGGA GAATCTGACC 
TTCAAGGAGC GGCAGCGTCT CTTCTCTCAG GGGAAGGAGG TTTCCAACAA GGTCAAAGCT 
TCGCGCAAAC TCATGGAGCT GGAGAACGAG CTCAACACCA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027140431.1
CDS	233..5677
Translation	MSGSREEERRKLADIINHWNANRLDLFEISRPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERQLDLDEKPLVVQLNWNKDDREGRFVLKNENDILPKKSQSNGPEKEKDGVIQNFKRTLSKKEKKKEKKREKEFARIPDGDEQALSREDGENSRLAAEVYKDMPENSFTRTISNPEVVMKRRRQQKLEKRMQEFMSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTRDTADFAVVEALEKYGLEKENPREYCIARQDDKSGKEAILDDTECPLQIFRDWPADRGALVFQLKKRPPDYQGRKGRKVDDKGLRGKDGSSSLPPEKLPYLVELSPGRGNHYAYYAYRHHEDGSDSRDKPKLYRLQHSITEVGSDCTEDGAIQLLGPGILPHHCNLMHSEGMVTVTPHGPDADTFVDGQRVTETTVLRSGSTLQFGQAHVFKFVDPMFDQGGKREPAAMMRSRHKSGSVPETTFDLHGDVHSGAALPTSKSSGKLEMERGMVKPMIRGEQQDGRSQDISADRAELTLPAGIEFRDNSEDTFLSAIINYTNSSTVHFKLSPTYVLYMACRYILSPSYRPDMSPSERTHKVIAIVNKMVSMMEGVIQEIPEGDQKQKNIAGALAFWMANASELLNFIKQDRDLNRITLDAQDILAHLVQMAFKYLVHCLQADLNNYMPAFLDDPEEHNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVTDTESGLCCHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVPNIHNTCFKLNSLQLHALMNNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRSLPNGLQDFLEPLLQRGFCRLVPHPRSPGTWTVHFEGADCDSHFSAADNSEMPMRKEPEVVTVTLKKHNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRDKNGKLRPKSEGFELYNSSVQNGSPESPQAGWDSYPEPKKMSGEDRLLKNRADHRSSPNVANQGQSPAGKAVYPGGPGTKITSVSTGNLCADDEPSPPRPEAYPIPTQTYPREYFTIPASKSQDRVVGPGQGQHWQGMEDRERLPVVDNIHNSMQRVNHSQEELYPPPGGLRPEERMQQHYQQDYQHRDMDYQHRELDYQRGGPPGVGGPDHWTPQPQVSSSLESSTSSQEHLNFSASSSSSNKTQNQKTGPGRWKTPNAPHSVPPHSAQPPSRSDLPPPPPPPPATYPESYDPYDPQPDLPLPPPPSAVTPAVAQQAADRKKREEQQRWYEKEKARLEEERERKRREQERKLGQIRANPVMPVQPHNNGGSMPPPHHQPPLPSAAPPQPYLPPAQPQPPPSRPEKLTSLPRSAVPPPASASNPAGMDTVIRELLPQQQPRTIERRDLQYITISKEELTSSDSLSPDPWKRDAREKAEKQQQLHIVDLLDKEIQELQAKPERTAEESDRLRKLMLEWQFQKRLQESKQSDEDEEEEDDEDVDTMMIMQRLEAEKRARQQNAVPAISVLDLLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERAKREAEEKRRQEEEYYTRLEAERRRQHDEAERKLLTPDDPAGFNSYTGNSAGVVGSGEVYKDPRDKWTKSQEQENSNPSGPPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNTK

Target ORF information:

RefSeq Version	XM_027284630.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027284630.1

ORF Insert Sequence:

ATGTCGGGCA GCAGGGAGGA GGAGAGGAGG AAACTGGCCG ACATCATCAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CGGCCCACAG AGGACCTGGA GTTCCATGGC 
GTGATGCGGT TCTACTTCCA GGACCGGGTC GCCGGTAACT TTGCCACCAA ATGCATCAGA 
GTGTCGAGCA CGGCGACAAC GCAGGACGTC ATCGAGACGT TAGCAGAGAA GTTCAGACCG 
GACATGAGGA TGCTCTCCTC GCCAAAATAC TCTTTGTACG AGGTCCACGT CAGTGGCGAA 
GAGCGTCAGC TGGATCTGGA CGAGAAGCCT CTGGTGGTTC AGCTGAACTG GAACAAAGAC 
GACAGAGAAG GACGCTTCGT CCTGAAGAAC GAGAACGACA TCCTCCCCAA GAAGAGTCAG 
AGTAACGGCC CGGAGAAGGA GAAGGACGGA GTGATCCAGA ACTTCAAGAG GACTTTGTCC 
AAGAAGGAGA AGAAGAAGGA GAAGAAGAGG GAGAAGGAGT TCGCCCGGAT CCCCGACGGA 
GACGAGCAGG CGCTCAGCCG AGAGGACGGG GAGAACAGTC GTCTGGCGGC GGAGGTTTAC 
AAGGACATGC CGGAGAACAG CTTCACCAGG ACGATCTCCA ACCCGGAGGT GGTGATGAAG 
AGGAGACGCC AGCAGAAGCT GGAGAAACGC ATGCAGGAGT TCATGAGCAG CGACGGGAGG 
CCCGACTCAG GGGGCACGTT GCGGATATAC GCCGACAGCC TGAAGCCGAA CATCCCGTAT 
AAAACCATCC TGCTGTCCAC GAGAGACACG GCTGACTTTG CGGTGGTGGA GGCGCTGGAG 
AAGTACGGGC TGGAGAAGGA GAACCCCCGA GAGTACTGCA TCGCCCGGCA AGACGATAAG 
TCCGGCAAGG AGGCGATCCT GGACGATACC GAGTGTCCAC TGCAGATCTT CAGGGACTGG 
CCTGCAGACA GAGGAGCGCT AGTGTTCCAG CTGAAGAAGA GACCCCCGGA CTACCAGGGC 
AGGAAGGGGA GGAAAGTGGA CGACAAGGGC CTGCGAGGGA AGGACGGCAG CAGCTCCCTG 
CCGCCCGAGA AACTGCCTTA CCTGGTGGAG CTGAGCCCAG GGCGAGGGAA TCACTATGCC 
TACTACGCCT ATCGGCACCA CGAAGACGGG TCTGACTCAC GGGACAAACC AAAGCTGTAC 
CGGCTCCAGC ACAGCATCAC TGAGGTCGGC TCAGACTGCA CAGAGGACGG AGCCATTCAG 
CTGCTCGGTC CGGGGATTCT GCCCCACCAC TGCAACCTCA TGCACAGTGA AGGCATGGTG 
ACGGTGACGC CGCACGGCCC CGACGCCGAC ACCTTCGTCG ACGGGCAACG GGTCACTGAG 
ACGACGGTGC TGCGCTCGGG CTCCACCCTC CAGTTTGGCC AAGCGCACGT CTTCAAGTTT 
GTGGACCCGA TGTTCGACCA GGGAGGGAAG AGAGAGCCGG CCGCCATGAT GAGGAGTCGA 
CACAAGTCCG GCAGCGTACC CGAAACCACC TTCGACCTTC ACGGAGACGT CCACAGCGGA 
GCCGCGCTGC CTACCAGCAA GAGCTCAGGA AAGCTGGAGA TGGAGAGAGG AATGGTCAAA 
CCGATGATCA GAGGAGAGCA GCAGGACGGC AGGTCTCAGG ACATTTCAGC AGACAGAGCC 
GAGTTGACTC TGCCAGCCGG CATCGAGTTC AGAGACAACT CTGAAGACAC CTTCCTGTCG 
GCCATCATCA ACTACACCAA CAGCTCCACG GTTCACTTCA AACTCTCGCC CACGTACGTC 
CTGTACATGG CCTGCCGCTA CATCCTGTCG CCCTCGTACC GGCCCGACAT GTCGCCGTCC 
GAGAGGACGC ACAAGGTCAT CGCCATCGTC AACAAGATGG TGAGCATGAT GGAGGGAGTC 
ATCCAGGAGA TTCCTGAAGG GGATCAGAAG CAGAAGAACA TCGCGGGAGC GCTGGCTTTC 
TGGATGGCCA ACGCCTCCGA GCTGCTCAAC TTCATCAAGC AGGACCGAGA CCTGAACCGC 
ATCACGCTGG ACGCCCAGGA CATCCTCGCC CATCTGGTTC AGATGGCTTT CAAGTACCTG 
GTCCACTGTC TGCAGGCTGA TCTGAATAAC TACATGCCGG CCTTCCTGGA CGACCCCGAG 
GAACACAACC CACAGAGACC CAAGATCGAG GACGTCCTGC ACACCCTCAC GGGGGCGATG 
TCGTTACTGC GGCGGTGTCG GGTCAACGCC GCTCTCACCA TCCAGCTCTT CTCGCAGCTC 
TTCCACTTCA TCAACATGTG GCTCTTCAAC AAGCTGGTGA CGGACACCGA GTCCGGGCTG 
TGCTGTCACT ACTGGGGGGC CATCCTGCGG CAGCAGCTCA GCCACATCGA GGCCTGGGCG 
GAGAAACAGG GGCTGGAGCT CGCCGCCGAC TGCCACCTCA GTCGAATCGT GCAGGCCACC 
ACCCTGCTGA CCATGGACAA ATACTCCATG CAGGATGTGC CGAACATCCA CAACACCTGC 
TTCAAGCTCA ACTCGCTGCA GCTCCACGCC CTCATGAACA ACTACCACTG CGCTCCGGAC 
GAGCCGTACA TCCCGCCGGA GCTGATAGAC CACGTGGTGG CAGTGGCAGA GAACACAGCA 
GATGAGCTGG CGAGGAGCGA CGGCAGAGAG GTTCAGCTGG AGGAGGATCC GGACCTCCAG 
CTGCCGTTCC TCCTTCCCGA GGACGGTTAC TCCTGCGACG TGGTGCGCAG CCTCCCCAAC 
GGCCTGCAGG ACTTCCTGGA GCCGCTGCTG CAGAGAGGTT TCTGTCGGTT GGTACCTCAC 
CCTCGCTCTC CCGGTACCTG GACGGTCCAT TTTGAAGGAG CTGACTGCGA CAGCCACTTC 
TCTGCTGCTG ACAACTCAGA GATGCCAATG AGGAAAGAGC CGGAGGTTGT CACGGTAACC 
CTGAAGAAGC ACAACGGCAT GGGCCTCAGC ATCGTGGCTG CCAAGGGTGC TGGTCAGGAG 
AAGCTGGGCA TCTACATCAA GTCTGTCGTC AAAGGAGGAG CAGCTGATGT GGACGGGCGT 
CTGGCTGCAG GTGACCAGCT GTTGAGCGTG GACGGGCGGA GTCTGGTCGG CCTATCACAA 
GAGAGGGCAG CGGAGCTGAT GACGAGGACG GGCTCAGTCG TGACTCTGGA GGTCGCCAAG 
CAGGGAGCCA TTTACCACGG ACTGGCCACG CTCCTCAACC AGCCGAGCCC CATGATGCCA 
CGAGCGTCAG ACAGAGGCCG CGACAAGAAC GGGAAACTCC GACCAAAGAG CGAAGGCTTC 
GAGTTGTACA ACAGCTCGGT GCAGAACGGC TCTCCGGAGA GCCCGCAGGC CGGCTGGGAC 
TCTTACCCCG AACCAAAGAA GATGAGCGGC GAAGATCGAC TCCTGAAGAA CCGAGCCGAC 
CACCGCTCCA GCCCCAACGT CGCCAATCAG GGCCAGAGTC CTGCAGGCAA AGCAGTGTAC 
CCCGGAGGAC CTGGCACCAA GATCACCTCC GTCTCCACCG GCAACCTGTG CGCAGACGAT 
GAGCCCTCCC CTCCACGTCC AGAAGCTTAC CCCATCCCCA CCCAGACCTA CCCCAGGGAG 
TACTTCACCA TCCCGGCTTC CAAGAGCCAG GATCGGGTCG TTGGTCCTGG GCAGGGACAG 
CACTGGCAGG GCATGGAGGA CAGAGAACGC CTCCCTGTGG TCGACAACAT CCACAACAGC 
ATGCAGCGGG TTAACCACTC CCAGGAGGAG TTGTACCCTC CACCCGGAGG CCTGAGGCCG 
GAGGAGCGCA TGCAGCAGCA CTACCAGCAG GACTACCAGC ACCGCGACAT GGACTACCAG 
CACAGAGAGC TCGACTACCA GAGAGGAGGT CCACCAGGTG TCGGAGGTCC CGATCACTGG 
ACTCCACAGC CTCAGGTGTC CTCCTCGCTG GAGTCGTCCA CGTCCAGCCA GGAGCACCTC 
AACTTCTCCG CCTCCTCCTC GTCCTCCAAC AAAACCCAGA ACCAGAAGAC CGGGCCGGGC 
CGGTGGAAGA CTCCCAACGC CCCTCACTCC GTGCCGCCAC ATTCAGCCCA GCCGCCCTCC 
CGCTCCGACC TGCCCCCTCC TCCGCCTCCA CCGCCAGCTA CTTACCCCGA ATCCTACGAC 
CCATACGACC CCCAACCGGA CCTGCCGCTC CCGCCGCCTC CCTCCGCCGT CACTCCTGCG 
GTAGCTCAGC AGGCGGCCGA CCGCAAGAAA CGTGAGGAGC AGCAGCGCTG GTATGAGAAG 
GAGAAAGCTC GGCTGGAGGA GGAGAGGGAG AGGAAGAGGA GAGAGCAGGA GAGGAAGCTG 
GGTCAGATCC GGGCCAACCC CGTCATGCCC GTCCAGCCTC ACAACAACGG AGGATCCATG 
CCTCCGCCTC ACCACCAGCC GCCTCTGCCG TCTGCAGCCC CGCCTCAGCC CTACCTTCCC 
CCCGCTCAGC CCCAGCCTCC ACCATCCAGA CCGGAGAAGC TGACCAGCCT GCCGCGGTCA 
GCCGTTCCTC CACCCGCCAG CGCCTCCAAC CCTGCAGGCA TGGACACGGT GATCAGGGAG 
CTGTTGCCAC AGCAGCAGCC GCGGACCATC GAGAGGCGAG ACCTGCAGTA CATCACCATC 
AGCAAGGAGG AGCTCACCTC CAGCGACAGT CTGTCTCCAG ACCCCTGGAA GCGAGACGCC 
CGCGAGAAGG CCGAGAAGCA GCAGCAGCTC CACATCGTCG ACCTGCTGGA CAAGGAGATC 
CAGGAGCTCC AGGCGAAGCC GGAGCGGACG GCGGAGGAGA GCGACCGCCT CAGGAAGCTG 
ATGCTGGAGT GGCAGTTCCA GAAACGACTG CAGGAGTCCA AGCAGAGCGA CGAAGACGAG 
GAGGAGGAGG ACGACGAGGA CGTGGACACC ATGATGATCA TGCAGAGGCT GGAGGCCGAG 
AAGAGAGCCA GGCAGCAGAA TGCTGTCCCA GCTATCTCTG TTCTAGACTT GTTGCAGGAC 
GAGGAGCGGC GGCGTAAGCA GCAGCTGGAG GAGATTCGTA AGCGGGAGGC GGAGGAGCGA 
GCAAAGCAGG AGGAGGAGAG GAGGTGGAGG GAGGAGGAGA GAGCCAAGCG AGAGGCCGAG 
GAGAAGAGGA GACAGGAGGA GGAGTACTAC ACTCGCCTGG AGGCGGAGCG GCGCCGGCAG 
CACGACGAGG CCGAGAGGAA GCTGCTGACG CCCGACGACC CGGCCGGATT TAACTCGTAC 
ACTGGAAACT CAGCAGGAGT TGTAGGATCA GGAGAAGTCT ACAAGGACCC GCGAGACAAA 
TGGACGAAGA GTCAGGAGCA GGAGAACTCG AACCCCAGCG GCCCTCCGGA GAATCTGACC 
TTCAAGGAGC GGCAGCGTCT CTTCTCTCAG GGGAAGGAGG TTTCCAACAA GGTCAAAGCT 
TCGCGCAAAC TCATGGAGCT GGAGAACGAG CTCAACACCA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afdn ( XM_027284631.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_027140432.1 Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X11, mRNA.

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CloneID	OLa200713
Clone ID Related Accession (Same CDS sequence)	XM_027284631.1
Accession Version	XM_027284631.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	afadin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040021.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGCA GCAGGGAGGA GGAGAGGAGG AAACTGGCCG ACATCATCAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CGGCCCACAG AGGACCTGGA GTTCCATGGC 
GTGATGCGGT TCTACTTCCA GGACCGGGTC GCCGGTAACT TTGCCACCAA ATGCATCAGA 
GTGTCGAGCA CGGCGACAAC GCAGGACGTC ATCGAGACGT TAGCAGAGAA GTTCAGACCG 
GACATGAGGA TGCTCTCCTC GCCAAAATAC TCTTTGTACG AGGTCCACGT CAGTGGCGAA 
GAGCGTCAGC TGGATCTGGA CGAGAAGCCT CTGGTGGTTC AGCTGAACTG GAACAAAGAC 
GACAGAGAAG GACGCTTCGT CCTGAAGAAC GAGAACGACA TCCTCCCCAA GAAGAGTCAG 
AGTAACGGCC CGGAGAAGGA GAAGGACGGA GTGATCCAGA ACTTCAAGAG GACTTTGTCC 
AAGAAGGAGA AGAAGAAGGA GAAGAAGAGG GAGAAGGAGT TCGCCCGGAT CCCCGACGGA 
GACGAGCAGG CGCTCAGCCG AGAGGACGGG GAGAACAGTC GTCTGGCGGC GGAGGTTTAC 
AAGGACATGC CGGAGAACAG CTTCACCAGG ACGATCTCCA ACCCGGAGGT GGTGATGAAG 
AGGAGACGCC AGCAGAAGCT GGAGAAACGC ATGCAGGAGT TCATGAGCAG CGACGGGAGG 
CCCGACTCAG GGGGCACGTT GCGGATATAC GCCGACAGCC TGAAGCCGAA CATCCCGTAT 
AAAACCATCC TGCTGTCCAC GAGAGACACG GCTGACTTTG CGGTGGTGGA GGCGCTGGAG 
AAGTACGGGC TGGAGAAGGA GAACCCCCGA GAGTACTGCA TCGCCCGGCA AGACGATAAG 
TCCGGCAAGG AGGCGATCCT GGACGATACC GAGTGTCCAC TGCAGATCTT CAGGGACTGG 
CCTGCAGACA GAGGAGCGCT AGTGTTCCAG CTGAAGAAGA GACCCCCGGA CTACCAGGGC 
AGGAAGGGGA GGAAAGTGGA CGACAAGGGC CTGCGAGGGA AGGACGGCAG CAGCTCCCTG 
CCGCCCGAGA AACTGCCTTA CCTGGTGGAG CTGAGCCCAG GGCGAGGGAA TCACTATGCC 
TACTACGCCT ATCGGCACCA CGAAGACGGG TCTGACTCAC GGGACAAACC AAAGCTGTAC 
CGGCTCCAGC ACAGCATCAC TGAGGTCGGC TCAGACTGCA CAGAGGACGG AGCCATTCAG 
CTGCTCGGTC CGGGGATTCT GCCCCACCAC TGCAACCTCA TGCACAGTGA AGGCATGGTG 
ACGGTGACGC CGCACGGCCC CGACGCCGAC ACCTTCGTCG ACGGGCAACG GGTCACTGAG 
ACGACGGTGC TGCGCTCGGG CTCCACCCTC CAGTTTGGCC AAGCGCACGT CTTCAAGTTT 
GTGGACCCGA TGTTCGACCA GGGAGGGAAG AGAGAGCCGG CCGCCATGAT GAGGAGTCGA 
CACAAGTCCG GCAGCGTACC CGAAACCACC TTCGACCTTC ACGGAGACGT CCACAGCGGA 
GCCGCGCTGC CTACCAGCAA GAGCTCAGGA AAGCTGGAGA TGGAGAGAGG AATGGTCAAA 
CCGATGATCA GAGGAGAGCA GCAGGACGGC AGGTCTCAGG ACATTTCAGC AGACAGAGCC 
GAGTTGACTC TGCCAGCCGG CATCGAGTTC AGAGACAACT CTGAAGACAC CTTCCTGTCG 
GCCATCATCA ACTACACCAA CAGCTCCACG GTTCACTTCA AACTCTCGCC CACGTACGTC 
CTGTACATGG CCTGCCGCTA CATCCTGTCG CCCTCGTACC GGCCCGACAT GTCGCCGTCC 
GAGAGGACGC ACAAGGTCAT CGCCATCGTC AACAAGATGG TGAGCATGAT GGAGGGAGTC 
ATCCAGGAGA TTCCTGAAGG GGATCAGAAG CAGAAGAACA TCGCGGGAGC GCTGGCTTTC 
TGGATGGCCA ACGCCTCCGA GCTGCTCAAC TTCATCAAGC AGGACCGAGA CCTGAACCGC 
ATCACGCTGG ACGCCCAGGA CATCCTCGCC CATCTGGTTC AGATGGCTTT CAAGTACCTG 
GTCCACTGTC TGCAGGCTGA TCTGAATAAC TACATGCCGG CCTTCCTGGA CGACCCCGAG 
GAACACAACC CACAGAGACC CAAGATCGAG GACGTCCTGC ACACCCTCAC GGGGGCGATG 
TCGTTACTGC GGCGGTGTCG GGTCAACGCC GCTCTCACCA TCCAGCTCTT CTCGCAGCTC 
TTCCACTTCA TCAACATGTG GCTCTTCAAC AAGCTGGTGA CGGACACCGA GTCCGGGCTG 
TGCTGTCACT ACTGGGGGGC CATCCTGCGG CAGCAGCTCA GCCACATCGA GGCCTGGGCG 
GAGAAACAGG GGCTGGAGCT CGCCGCCGAC TGCCACCTCA GTCGAATCGT GCAGGCCACC 
ACCCTGCTGA CCATGGACAA ATACTCCATG CAGGATGTGC CGAACATCCA CAACACCTGC 
TTCAAGCTCA ACTCGCTGCA GCTCCACGCC CTCATGAACA ACTACCACTG CGCTCCGGAC 
GAGCCGTACA TCCCGCCGGA GCTGATAGAC CACGTGGTGG CAGTGGCAGA GAACACAGCA 
GATGAGCTGG CGAGGAGCGA CGGCAGAGAG GTTCAGCTGG AGGAGGATCC GGACCTCCAG 
CTGCCGTTCC TCCTTCCCGA GGACGGTTAC TCCTGCGACG TGGTGCGCAG CCTCCCCAAC 
GGCCTGCAGG ACTTCCTGGA GCCGCTGCTG CAGAGAGGTT TCTGTCGGTT GGTACCTCAC 
CCTCGCTCTC CCGGTACCTG GACGGTCCAT TTTGAAGGAG CTGACTGCGA CAGCCACTTC 
TCTGCTGCTG ACAACTCAGA GATGCCAATG AGGAAAGAGC CGGAGGTTGT CACGGTAACC 
CTGAAGAAGC ACAACGGCAT GGGCCTCAGC ATCGTGGCTG CCAAGGGTGC TGGTCAGGAG 
AAGCTGGGCA TCTACATCAA GTCTGTCGTC AAAGGAGGAG CAGCTGATGT GGACGGGCGT 
CTGGCTGCAG GTGACCAGCT GTTGAGCGTG GACGGGCGGA GTCTGGTCGG CCTATCACAA 
GAGAGGGCAG CGGAGCTGAT GACGAGGACG GGCTCAGTCG TGACTCTGGA GGTCGCCAAG 
CAGGGAGCCA TTTACCACGG ACTGGCCACG CTCCTCAACC AGCCGAGCCC CATGATGCCA 
CGAGCGTCAG ACAGAGGCCG CGACAAGAAC GGGAAACTCC GACCAAAGAG CGAAGGCTTC 
GAGTTGTACA ACAGCTCGGT GCAGAACGGC TCTCCGGAGA GCCCGCAGGC CGGCTGGGAC 
TCTTACCCCG AACCAAAGAA GATGAGCGGC GAAGATCGAC TCCTGAAGAA CCGAGCCGAC 
CACCGCTCCA GCCCCAACGT CGCCAATCAG GGCCAGAGTC CTGCAGGCAA AGCAGTGTAC 
CCCGGAGGAC CTGGCACCAA GATCACCTCC GTCTCCACCG GCAACCTGTG CGCAGACGAT 
GAGCCCTCCC CTCCACGTCC AGAAGCTTAC CCCATCCCCA CCCAGACCTA CCCCAGGGAG 
TACTTCACCA TCCCGGCTTC CAAGAGCCAG GATCGGGTCG TTGGTCCTGG GCAGGGACAG 
CACTGGCAGG GCATGGAGGA CAGAGAACGC CTCCCTGTGG TCGACAACAT CCACAACAGC 
ATGCAGCGGG TTAACCACTC CCAGGAGGAG TTGTACCCTC CACCCGGAGG CCTGAGGCCG 
GAGGAGCGCA TGCAGCAGCA CTACCAGCAG GACTACCAGC ACCGCGACAT GGACTACCAG 
CACAGAGAGC TCGACTACCA GAGAGGAGGT CCACCAGGTG TCGGAGGTCC CGATCACTGG 
ACTCCACAGC CTCAGGTGTC CTCCTCGCTG GAGTCGTCCA CGTCCAGCCA GGAGCACCTC 
AACTTCTCCG CCTCCTCCTC GTCCTCCAAC AAAACCCAGA ACCAGAAGAC CGGGCCGGGC 
CGGTGGAAGA CTCCCAACGC CCCTCACTCC GTGCCGCCAC ATTCAGCCCA GCCGCCCTCC 
CGCTCCGACC TGCCCCCTCC TCCGCCTCCA CCGCCAGCTA CTTACCCCGA ATCCTACGAC 
CCATACGACC CCCAACCGGA CCTGCCGCTC CCGCCGCCTC CCTCCGCCGT CACTCCTGCG 
GTAGCTCAGC AGGCGGCCGA CCGCAAGAAA CGTGAGGAGC AGCAGCGCTG GTATGAGAAG 
GAGAAAGCTC GGCTGGAGGA GGAGAGGGAG AGGAAGAGGA GAGAGCAGGA GAGGAAGCTG 
GGTCAGATCC GGGCCAACCC CGTCATGCCC GTCCAGCCTC ACAACAACGG AGGATCCATG 
CCTCCGCCTC ACCACCAGCC GCCTCTGCCG TCTGCAGCCC CGCCTCAGCC CTACCTTCCC 
CCCGCTCAGC CCCAGCCTCC ACCATCCAGA CCGGAGAAGC TGACCAGCCT GCCGCGGTCA 
GCCGTTCCTC CACCCGCCAG CGCCTCCAAC CCTGCAGGCA TGGACACGGT GATCAGGGAG 
CTGTTGCCAC AGCAGCAGCC GCGGACCATC GAGAGGCGAG ACCTGCAGTA CATCACCATC 
AGCAAGGAGG AGCTCACCTC CAGCGACAGT CTGTCTCCAG ACCCCTGGAA GCGAGACGCC 
CGCGAGAAGG CCGAGAAGCA GCAGCAGCTC CACATCGTCG ACCTGCTGGA CAAGGAGATC 
CAGGAGCTCC AGGCGAAGCC GGAGCGGACG GCGGAGGAGA GCGACCGCCT CAGGAAGCTG 
ATGCTGGAGT GGCAGTTCCA GAAACGACTG CAGGAGTCCA AGCAGAGCGA CGAAGACGAG 
GAGGAGGAGG ACGACGAGGA CGTGGACACC ATGATGATCA TGCAGAGGCT GGAGGCCGAG 
AAGAGAGCCA GGTTGCAGGA CGAGGAGCGG CGGCGTAAGC AGCAGCTGGA GGAGATTCGT 
AAGCGGGAGG CGGAGGAGCG AGCAAAGCAG GAGGAGGAGA GGAGGTGGAG GGAGGAGGAG 
AGAGCCAAGC GAGAGGCCGA GGAGAAGAGG AGACAGGAGG AGGAGTACTA CACTCGCCTG 
GAGGCGGAGC GGCGCCGGCA GCACGACGAG GCCGAGAGGA AGCTGCTGAC GCCCGACGAC 
CCGGCCGGAT TTAACTCGTA CACTGGAAAC TCAGCAGGAG TTGTAGGATC AGGAGAAGTC 
TACAAGGACC CGCGAGACAA ATGGACGAAG AGTCAGGAGC AGGAGAACTC GAACCCCAGC 
GGCCCTCCGG AGAATCTGAC CTTCAAGGAG CGGCAGCGTC TCTTCTCTCA GGGGAAGGAG 
GTTTCCAACA AGGTCAAAGC TTCGCGCAAA CTCATGGAGC TGGAGAACGA GCTCAACACC 
AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027140432.1
CDS	232..5637
Translation	MSGSREEERRKLADIINHWNANRLDLFEISRPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERQLDLDEKPLVVQLNWNKDDREGRFVLKNENDILPKKSQSNGPEKEKDGVIQNFKRTLSKKEKKKEKKREKEFARIPDGDEQALSREDGENSRLAAEVYKDMPENSFTRTISNPEVVMKRRRQQKLEKRMQEFMSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTRDTADFAVVEALEKYGLEKENPREYCIARQDDKSGKEAILDDTECPLQIFRDWPADRGALVFQLKKRPPDYQGRKGRKVDDKGLRGKDGSSSLPPEKLPYLVELSPGRGNHYAYYAYRHHEDGSDSRDKPKLYRLQHSITEVGSDCTEDGAIQLLGPGILPHHCNLMHSEGMVTVTPHGPDADTFVDGQRVTETTVLRSGSTLQFGQAHVFKFVDPMFDQGGKREPAAMMRSRHKSGSVPETTFDLHGDVHSGAALPTSKSSGKLEMERGMVKPMIRGEQQDGRSQDISADRAELTLPAGIEFRDNSEDTFLSAIINYTNSSTVHFKLSPTYVLYMACRYILSPSYRPDMSPSERTHKVIAIVNKMVSMMEGVIQEIPEGDQKQKNIAGALAFWMANASELLNFIKQDRDLNRITLDAQDILAHLVQMAFKYLVHCLQADLNNYMPAFLDDPEEHNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVTDTESGLCCHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVPNIHNTCFKLNSLQLHALMNNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRSLPNGLQDFLEPLLQRGFCRLVPHPRSPGTWTVHFEGADCDSHFSAADNSEMPMRKEPEVVTVTLKKHNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRDKNGKLRPKSEGFELYNSSVQNGSPESPQAGWDSYPEPKKMSGEDRLLKNRADHRSSPNVANQGQSPAGKAVYPGGPGTKITSVSTGNLCADDEPSPPRPEAYPIPTQTYPREYFTIPASKSQDRVVGPGQGQHWQGMEDRERLPVVDNIHNSMQRVNHSQEELYPPPGGLRPEERMQQHYQQDYQHRDMDYQHRELDYQRGGPPGVGGPDHWTPQPQVSSSLESSTSSQEHLNFSASSSSSNKTQNQKTGPGRWKTPNAPHSVPPHSAQPPSRSDLPPPPPPPPATYPESYDPYDPQPDLPLPPPPSAVTPAVAQQAADRKKREEQQRWYEKEKARLEEERERKRREQERKLGQIRANPVMPVQPHNNGGSMPPPHHQPPLPSAAPPQPYLPPAQPQPPPSRPEKLTSLPRSAVPPPASASNPAGMDTVIRELLPQQQPRTIERRDLQYITISKEELTSSDSLSPDPWKRDAREKAEKQQQLHIVDLLDKEIQELQAKPERTAEESDRLRKLMLEWQFQKRLQESKQSDEDEEEEDDEDVDTMMIMQRLEAEKRARLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERAKREAEEKRRQEEEYYTRLEAERRRQHDEAERKLLTPDDPAGFNSYTGNSAGVVGSGEVYKDPRDKWTKSQEQENSNPSGPPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNTK

Target ORF information:

RefSeq Version	XM_027284631.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027284631.1

ORF Insert Sequence:

ATGTCGGGCA GCAGGGAGGA GGAGAGGAGG AAACTGGCCG ACATCATCAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CGGCCCACAG AGGACCTGGA GTTCCATGGC 
GTGATGCGGT TCTACTTCCA GGACCGGGTC GCCGGTAACT TTGCCACCAA ATGCATCAGA 
GTGTCGAGCA CGGCGACAAC GCAGGACGTC ATCGAGACGT TAGCAGAGAA GTTCAGACCG 
GACATGAGGA TGCTCTCCTC GCCAAAATAC TCTTTGTACG AGGTCCACGT CAGTGGCGAA 
GAGCGTCAGC TGGATCTGGA CGAGAAGCCT CTGGTGGTTC AGCTGAACTG GAACAAAGAC 
GACAGAGAAG GACGCTTCGT CCTGAAGAAC GAGAACGACA TCCTCCCCAA GAAGAGTCAG 
AGTAACGGCC CGGAGAAGGA GAAGGACGGA GTGATCCAGA ACTTCAAGAG GACTTTGTCC 
AAGAAGGAGA AGAAGAAGGA GAAGAAGAGG GAGAAGGAGT TCGCCCGGAT CCCCGACGGA 
GACGAGCAGG CGCTCAGCCG AGAGGACGGG GAGAACAGTC GTCTGGCGGC GGAGGTTTAC 
AAGGACATGC CGGAGAACAG CTTCACCAGG ACGATCTCCA ACCCGGAGGT GGTGATGAAG 
AGGAGACGCC AGCAGAAGCT GGAGAAACGC ATGCAGGAGT TCATGAGCAG CGACGGGAGG 
CCCGACTCAG GGGGCACGTT GCGGATATAC GCCGACAGCC TGAAGCCGAA CATCCCGTAT 
AAAACCATCC TGCTGTCCAC GAGAGACACG GCTGACTTTG CGGTGGTGGA GGCGCTGGAG 
AAGTACGGGC TGGAGAAGGA GAACCCCCGA GAGTACTGCA TCGCCCGGCA AGACGATAAG 
TCCGGCAAGG AGGCGATCCT GGACGATACC GAGTGTCCAC TGCAGATCTT CAGGGACTGG 
CCTGCAGACA GAGGAGCGCT AGTGTTCCAG CTGAAGAAGA GACCCCCGGA CTACCAGGGC 
AGGAAGGGGA GGAAAGTGGA CGACAAGGGC CTGCGAGGGA AGGACGGCAG CAGCTCCCTG 
CCGCCCGAGA AACTGCCTTA CCTGGTGGAG CTGAGCCCAG GGCGAGGGAA TCACTATGCC 
TACTACGCCT ATCGGCACCA CGAAGACGGG TCTGACTCAC GGGACAAACC AAAGCTGTAC 
CGGCTCCAGC ACAGCATCAC TGAGGTCGGC TCAGACTGCA CAGAGGACGG AGCCATTCAG 
CTGCTCGGTC CGGGGATTCT GCCCCACCAC TGCAACCTCA TGCACAGTGA AGGCATGGTG 
ACGGTGACGC CGCACGGCCC CGACGCCGAC ACCTTCGTCG ACGGGCAACG GGTCACTGAG 
ACGACGGTGC TGCGCTCGGG CTCCACCCTC CAGTTTGGCC AAGCGCACGT CTTCAAGTTT 
GTGGACCCGA TGTTCGACCA GGGAGGGAAG AGAGAGCCGG CCGCCATGAT GAGGAGTCGA 
CACAAGTCCG GCAGCGTACC CGAAACCACC TTCGACCTTC ACGGAGACGT CCACAGCGGA 
GCCGCGCTGC CTACCAGCAA GAGCTCAGGA AAGCTGGAGA TGGAGAGAGG AATGGTCAAA 
CCGATGATCA GAGGAGAGCA GCAGGACGGC AGGTCTCAGG ACATTTCAGC AGACAGAGCC 
GAGTTGACTC TGCCAGCCGG CATCGAGTTC AGAGACAACT CTGAAGACAC CTTCCTGTCG 
GCCATCATCA ACTACACCAA CAGCTCCACG GTTCACTTCA AACTCTCGCC CACGTACGTC 
CTGTACATGG CCTGCCGCTA CATCCTGTCG CCCTCGTACC GGCCCGACAT GTCGCCGTCC 
GAGAGGACGC ACAAGGTCAT CGCCATCGTC AACAAGATGG TGAGCATGAT GGAGGGAGTC 
ATCCAGGAGA TTCCTGAAGG GGATCAGAAG CAGAAGAACA TCGCGGGAGC GCTGGCTTTC 
TGGATGGCCA ACGCCTCCGA GCTGCTCAAC TTCATCAAGC AGGACCGAGA CCTGAACCGC 
ATCACGCTGG ACGCCCAGGA CATCCTCGCC CATCTGGTTC AGATGGCTTT CAAGTACCTG 
GTCCACTGTC TGCAGGCTGA TCTGAATAAC TACATGCCGG CCTTCCTGGA CGACCCCGAG 
GAACACAACC CACAGAGACC CAAGATCGAG GACGTCCTGC ACACCCTCAC GGGGGCGATG 
TCGTTACTGC GGCGGTGTCG GGTCAACGCC GCTCTCACCA TCCAGCTCTT CTCGCAGCTC 
TTCCACTTCA TCAACATGTG GCTCTTCAAC AAGCTGGTGA CGGACACCGA GTCCGGGCTG 
TGCTGTCACT ACTGGGGGGC CATCCTGCGG CAGCAGCTCA GCCACATCGA GGCCTGGGCG 
GAGAAACAGG GGCTGGAGCT CGCCGCCGAC TGCCACCTCA GTCGAATCGT GCAGGCCACC 
ACCCTGCTGA CCATGGACAA ATACTCCATG CAGGATGTGC CGAACATCCA CAACACCTGC 
TTCAAGCTCA ACTCGCTGCA GCTCCACGCC CTCATGAACA ACTACCACTG CGCTCCGGAC 
GAGCCGTACA TCCCGCCGGA GCTGATAGAC CACGTGGTGG CAGTGGCAGA GAACACAGCA 
GATGAGCTGG CGAGGAGCGA CGGCAGAGAG GTTCAGCTGG AGGAGGATCC GGACCTCCAG 
CTGCCGTTCC TCCTTCCCGA GGACGGTTAC TCCTGCGACG TGGTGCGCAG CCTCCCCAAC 
GGCCTGCAGG ACTTCCTGGA GCCGCTGCTG CAGAGAGGTT TCTGTCGGTT GGTACCTCAC 
CCTCGCTCTC CCGGTACCTG GACGGTCCAT TTTGAAGGAG CTGACTGCGA CAGCCACTTC 
TCTGCTGCTG ACAACTCAGA GATGCCAATG AGGAAAGAGC CGGAGGTTGT CACGGTAACC 
CTGAAGAAGC ACAACGGCAT GGGCCTCAGC ATCGTGGCTG CCAAGGGTGC TGGTCAGGAG 
AAGCTGGGCA TCTACATCAA GTCTGTCGTC AAAGGAGGAG CAGCTGATGT GGACGGGCGT 
CTGGCTGCAG GTGACCAGCT GTTGAGCGTG GACGGGCGGA GTCTGGTCGG CCTATCACAA 
GAGAGGGCAG CGGAGCTGAT GACGAGGACG GGCTCAGTCG TGACTCTGGA GGTCGCCAAG 
CAGGGAGCCA TTTACCACGG ACTGGCCACG CTCCTCAACC AGCCGAGCCC CATGATGCCA 
CGAGCGTCAG ACAGAGGCCG CGACAAGAAC GGGAAACTCC GACCAAAGAG CGAAGGCTTC 
GAGTTGTACA ACAGCTCGGT GCAGAACGGC TCTCCGGAGA GCCCGCAGGC CGGCTGGGAC 
TCTTACCCCG AACCAAAGAA GATGAGCGGC GAAGATCGAC TCCTGAAGAA CCGAGCCGAC 
CACCGCTCCA GCCCCAACGT CGCCAATCAG GGCCAGAGTC CTGCAGGCAA AGCAGTGTAC 
CCCGGAGGAC CTGGCACCAA GATCACCTCC GTCTCCACCG GCAACCTGTG CGCAGACGAT 
GAGCCCTCCC CTCCACGTCC AGAAGCTTAC CCCATCCCCA CCCAGACCTA CCCCAGGGAG 
TACTTCACCA TCCCGGCTTC CAAGAGCCAG GATCGGGTCG TTGGTCCTGG GCAGGGACAG 
CACTGGCAGG GCATGGAGGA CAGAGAACGC CTCCCTGTGG TCGACAACAT CCACAACAGC 
ATGCAGCGGG TTAACCACTC CCAGGAGGAG TTGTACCCTC CACCCGGAGG CCTGAGGCCG 
GAGGAGCGCA TGCAGCAGCA CTACCAGCAG GACTACCAGC ACCGCGACAT GGACTACCAG 
CACAGAGAGC TCGACTACCA GAGAGGAGGT CCACCAGGTG TCGGAGGTCC CGATCACTGG 
ACTCCACAGC CTCAGGTGTC CTCCTCGCTG GAGTCGTCCA CGTCCAGCCA GGAGCACCTC 
AACTTCTCCG CCTCCTCCTC GTCCTCCAAC AAAACCCAGA ACCAGAAGAC CGGGCCGGGC 
CGGTGGAAGA CTCCCAACGC CCCTCACTCC GTGCCGCCAC ATTCAGCCCA GCCGCCCTCC 
CGCTCCGACC TGCCCCCTCC TCCGCCTCCA CCGCCAGCTA CTTACCCCGA ATCCTACGAC 
CCATACGACC CCCAACCGGA CCTGCCGCTC CCGCCGCCTC CCTCCGCCGT CACTCCTGCG 
GTAGCTCAGC AGGCGGCCGA CCGCAAGAAA CGTGAGGAGC AGCAGCGCTG GTATGAGAAG 
GAGAAAGCTC GGCTGGAGGA GGAGAGGGAG AGGAAGAGGA GAGAGCAGGA GAGGAAGCTG 
GGTCAGATCC GGGCCAACCC CGTCATGCCC GTCCAGCCTC ACAACAACGG AGGATCCATG 
CCTCCGCCTC ACCACCAGCC GCCTCTGCCG TCTGCAGCCC CGCCTCAGCC CTACCTTCCC 
CCCGCTCAGC CCCAGCCTCC ACCATCCAGA CCGGAGAAGC TGACCAGCCT GCCGCGGTCA 
GCCGTTCCTC CACCCGCCAG CGCCTCCAAC CCTGCAGGCA TGGACACGGT GATCAGGGAG 
CTGTTGCCAC AGCAGCAGCC GCGGACCATC GAGAGGCGAG ACCTGCAGTA CATCACCATC 
AGCAAGGAGG AGCTCACCTC CAGCGACAGT CTGTCTCCAG ACCCCTGGAA GCGAGACGCC 
CGCGAGAAGG CCGAGAAGCA GCAGCAGCTC CACATCGTCG ACCTGCTGGA CAAGGAGATC 
CAGGAGCTCC AGGCGAAGCC GGAGCGGACG GCGGAGGAGA GCGACCGCCT CAGGAAGCTG 
ATGCTGGAGT GGCAGTTCCA GAAACGACTG CAGGAGTCCA AGCAGAGCGA CGAAGACGAG 
GAGGAGGAGG ACGACGAGGA CGTGGACACC ATGATGATCA TGCAGAGGCT GGAGGCCGAG 
AAGAGAGCCA GGTTGCAGGA CGAGGAGCGG CGGCGTAAGC AGCAGCTGGA GGAGATTCGT 
AAGCGGGAGG CGGAGGAGCG AGCAAAGCAG GAGGAGGAGA GGAGGTGGAG GGAGGAGGAG 
AGAGCCAAGC GAGAGGCCGA GGAGAAGAGG AGACAGGAGG AGGAGTACTA CACTCGCCTG 
GAGGCGGAGC GGCGCCGGCA GCACGACGAG GCCGAGAGGA AGCTGCTGAC GCCCGACGAC 
CCGGCCGGAT TTAACTCGTA CACTGGAAAC TCAGCAGGAG TTGTAGGATC AGGAGAAGTC 
TACAAGGACC CGCGAGACAA ATGGACGAAG AGTCAGGAGC AGGAGAACTC GAACCCCAGC 
GGCCCTCCGG AGAATCTGAC CTTCAAGGAG CGGCAGCGTC TCTTCTCTCA GGGGAAGGAG 
GTTTCCAACA AGGTCAAAGC TTCGCGCAAA CTCATGGAGC TGGAGAACGA GCTCAACACC 
AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afdn ( XM_027284632.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_027140433.1 Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X12, mRNA.

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CloneID	OLa200714
Clone ID Related Accession (Same CDS sequence)	XM_027284632.1
Accession Version	XM_027284632.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	afadin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040021.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGCA GCAGGGAGGA GGAGAGGAGG AAACTGGCCG ACATCATCAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CGGCCCACAG AGGACCTGGA GTTCCATGGC 
GTGATGCGGT TCTACTTCCA GGACCGGGTC GCCGGTAACT TTGCCACCAA ATGCATCAGA 
GTGTCGAGCA CGGCGACAAC GCAGGACGTC ATCGAGACGT TAGCAGAGAA GTTCAGACCG 
GACATGAGGA TGCTCTCCTC GCCAAAATAC TCTTTGTACG AGGTCCACGT CAGTGGCGAA 
GAGCGTCAGC TGGATCTGGA CGAGAAGCCT CTGGTGGTTC AGCTGAACTG GAACAAAGAC 
GACAGAGAAG GACGCTTCGT CCTGAAGAAC GAGAACGACA TCCTCCCCAA GAAGAGTCAG 
AGTAACGGCC CGGAGAAGGA GAAGGACGGA GTGATCCAGA ACTTCAAGAG GACTTTGTCC 
AAGAAGGAGA AGAAGAAGGA GAAGAAGAGG GAGAAGGAGT TCGCCCGGAT CCCCGACGGA 
GACGAGCAGG CGCTCAGCCG AGAGGACGGG GAGAACAGTC GTCTGGCGGC GGAGGTTTAC 
AAGGACATGC CGGAGAACAG CTTCACCAGG ACGATCTCCA ACCCGGAGGT GGTGATGAAG 
AGGAGACGCC AGCAGAAGCT GGAGAAACGC ATGCAGGAGT TCATGAGCAG CGACGGGAGG 
CCCGACTCAG GGGGCACGTT GCGGATATAC GCCGACAGCC TGAAGCCGAA CATCCCGTAT 
AAAACCATCC TGCTGTCCAC GAGAGACACG GCTGACTTTG CGGTGGTGGA GGCGCTGGAG 
AAGTACGGGC TGGAGAAGGA GAACCCCCGA GAGTACTGCA TCGCCCGGCA AGACGATAAG 
TCCGGCAAGG AGGCGATCCT GGACGATACC GAGTGTCCAC TGCAGATCTT CAGGGACTGG 
CCTGCAGACA GAGGAGCGCT AGTGTTCCAG CTGAAGAAGA GACCCCCGGA CTACCAGGGC 
AGGAAGGGGA GGAAAGTGGA CGACAAGGGC CTGCGAGGGA AGGACGGCAG CAGCTCCCTG 
CCGCCCGAGA AACTGCCTTA CCTGGTGGAG CTGAGCCCAG GGCGAGGGAA TCACTATGCC 
TACTACGCCT ATCGGCACCA CGAAGACGGG TCTGACTCAC GGGACAAACC AAAGCTGTAC 
CGGCTCCAGC ACAGCATCAC TGAGGTCGGC TCAGACTGCA CAGAGGACGG AGCCATTCAG 
CTGCTCGGTC CGGGGATTCT GCCCCACCAC TGCAACCTCA TGCACAGTGA AGGCATGGTG 
ACGGTGACGC CGCACGGCCC CGACGCCGAC ACCTTCGTCG ACGGGCAACG GGTCACTGAG 
ACGACGGTGC TGCGCTCGGG CTCCACCCTC CAGTTTGGCC AAGCGCACGT CTTCAAGTTT 
GTGGACCCGA TGTTCGACCA GGGAGGGAAG AGAGAGCCGG CCGCCATGAT GAGGAGTCGA 
CACAAGTCCG GCAGCGTACC CGAAACCACC TTCGACCTTC ACGGAGACGT CCACAGCGGA 
GCCGCGCTGC CTACCAGCAA GAGCTCAGGA AAGCTGGAGA TGGAGAGAGG AATGGTCAAA 
CCGATGATCA GAGGAGAGCA GCAGGACGGC AGGTCTCAGG ACATTTCAGC AGACAGAGCC 
GAGTTGACTC TGCCAGCCGG CATCGAGTTC AGAGACAACT CTGAAGACAC CTTCCTGTCG 
GCCATCATCA ACTACACCAA CAGCTCCACG GTTCACTTCA AACTCTCGCC CACGTACGTC 
CTGTACATGG CCTGCCGCTA CATCCTGTCG CCCTCGTACC GGCCCGACAT GTCGCCGTCC 
GAGAGGACGC ACAAGGTCAT CGCCATCGTC AACAAGATGG TGAGCATGAT GGAGGGAGTC 
ATCCAGGAGA TTCCTGAAGG GGATCAGAAG CAGAAGAACA TCGCGGGAGC GCTGGCTTTC 
TGGATGGCCA ACGCCTCCGA GCTGCTCAAC TTCATCAAGC AGGACCGAGA CCTGAACCGC 
ATCACGCTGG ACGCCCAGGA CATCCTCGCC CATCTGGTTC AGATGGCTTT CAAGTACCTG 
GTCCACTGTC TGCAGGCTGA TCTGAATAAC TACATGCCGG CCTTCCTGGA CGACCCCGAG 
GAACACAACC CACAGAGACC CAAGATCGAG GACGTCCTGC ACACCCTCAC GGGGGCGATG 
TCGTTACTGC GGCGGTGTCG GGTCAACGCC GCTCTCACCA TCCAGCTCTT CTCGCAGCTC 
TTCCACTTCA TCAACATGTG GCTCTTCAAC AAGCTGGTGA CGGACACCGA GTCCGGGCTG 
TGCTGTCACT ACTGGGGGGC CATCCTGCGG CAGCAGCTCA GCCACATCGA GGCCTGGGCG 
GAGAAACAGG GGCTGGAGCT CGCCGCCGAC TGCCACCTCA GTCGAATCGT GCAGGCCACC 
ACCCTGCTGA CCATGGACAA ATACTCCATG CAGGATGTGC CGAACATCCA CAACACCTGC 
TTCAAGCTCA ACTCGCTGCA GCTCCACGCC CTCATGAACA ACTACCACTG CGCTCCGGAC 
GAGCCGTACA TCCCGCCGGA GCTGATAGAC CACGTGGTGG CAGTGGCAGA GAACACAGCA 
GATGAGCTGG CGAGGAGCGA CGGCAGAGAG GTTCAGCTGG AGGAGGATCC GGACCTCCAG 
CTGCCGTTCC TCCTTCCCGA GGACGGTTAC TCCTGCGACG TGGTGCGCAG CCTCCCCAAC 
GGCCTGCAGG ACTTCCTGGA GCCGCTGCTG CAGAGAGGTT TCTGTCGGTT GGTACCTCAC 
CCTCGCTCTC CCGGTACCTG GACGGTCCAT TTTGAAGGAG CTGACTGCGA CAGCCACTTC 
TCTGCTGCTG ACAACTCAGA GATGCCAATG AGGAAAGAGC CGGAGGTTGT CACGGTAACC 
CTGAAGAAGC ACAACGGCAT GGGCCTCAGC ATCGTGGCTG CCAAGGGTGC TGGTCAGGAG 
AAGCTGGGCA TCTACATCAA GTCTGTCGTC AAAGGAGGAG CAGCTGATGT GGACGGGCGT 
CTGGCTGCAG GTGACCAGCT GTTGAGCGTG GACGGGCGGA GTCTGGTCGG CCTATCACAA 
GAGAGGGCAG CGGAGCTGAT GACGAGGACG GGCTCAGTCG TGACTCTGGA GGTCGCCAAG 
CAGGGAGCCA TTTACCACGG ACTGGCCACG CTCCTCAACC AGCCGAGCCC CATGATGCCA 
CGAGCGTCAG ACAGAGGCCG CGACAAGAAC GGGAAACTCC GACCAAAGAG CGAAGGCTTC 
GAGTTGTACA ACAGCTCGGT GCAGAACGGC TCTCCGGAGA GCCCGCAGGC CGGCTGGGAC 
TCTTACCCCG AACCAAAGAA GATGAGCGGC GAAGATCGAC TCCTGAAGAA CCGAGCCGAC 
CACCGCTCCA GCCCCAACGT CGCCAATCAG GGCCAGAGTC CTGCAGGCAA AGCAGTGTAC 
CCCGGAGGAC CTGGCACCAA GATCACCTCC GTCTCCACCG GCAACCTGTG CGCAGACGAT 
GAGCCCTCCC CTCCACGTCC AGAAGCTTAC CCCATCCCCA CCCAGACCTA CCCCAGGGAG 
TACTTCACCA TCCCGGCTTC CAAGAGCCAG GATCGGGTCG TTGGTCCTGG GCAGGGACAG 
CACTGGCAGG GCATGGAGGA CAGAGAACGC CTCCCTGTGG TCGACAACAT CCACAACAGC 
ATGCAGCGGG TTAACCACTC CCAGGAGGAG TTGTACCCTC CACCCGGAGG CCTGAGGCCG 
GAGGAGCGCA TGCAGCAGCA CTACCAGCAG GACTACCAGC ACCGCGACAT GGACTACCAG 
CACAGAGAGC TCGACTACCA GAGAGGAGGT CCACCAGGTG TCGGAGGTCC CGATCACTGG 
ACTCCACAGC CTCAGGTGTC CTCCTCGCTG GAGTCGTCCA CGTCCAGCCA GGAGCACCTC 
AACTTCTCCG CCTCCTCCTC GTCCTCCAAC AAAACCCAGA ACCAGAAGAC CGGGCCGGGC 
CGGTGGAAGA CTCCCAACGC CCCTCACTCC GTGCCGCCAC ATTCAGCCCA GCCGCCCTCC 
CGCTCCGACC TGCCCCCTCC TCCGCCTCCA CCGCCAGCTA CTTACCCCGA ATCCTACGAC 
CCATACGACC CCCAACCGGA CCTGCCGCTC CCGCCGCCTC CCTCCGCCGT CACTCCTGCG 
GTAGCTCAGC AGGCGGCCGA CCGCAAGAAA CGTGAGGAGC AGCAGCGCTG GTATGAGAAG 
GAGAAAGCTC GGCTGGAGGA GGAGAGGGAG AGGAAGAGGA GAGAGCAGGA GAGGAAGCTG 
GGTCAGATCC GGGCCAACCC CGTCATGCCC GTCCAGCCTC ACAACAACGG AGGATCCATG 
CCTCCGCCTC ACCACCAGCC GCCTCTGCCG TCTGCAGCCC CGCCTCAGCC CTACCTTCCC 
CCCGCTCAGC CCCAGCCTCC ACCATCCAGA CCGGAGAAGC TGACCAGCCT GCCGCGGTCA 
GCCGTTCCTC CACCCGCCAG CGCCTCCAAC CCTGCAGGCA TGGACACGGT GATCAGGGAG 
CTGTTGCCAC AGCAGCAGCC GCGGACCATC GAGAGGCGAG ACCTGCAGTA CATCACCATC 
AGCAAGGAGG AGCTCACCTC CAGCGACAGT CTGTCTCCAG ACCCCTGGAA GCGAGACGCC 
CGCGAGAAGG CCGAGAAGCA GCAGCAGCTC CACATCGTCG ACCTGCTGGA CAAGGAGATC 
CAGGAGCTCC AGGCGAAGCC GGAGCGGACG GCGGAGGAGA GCGACCGCCT CAGGAAGCTG 
ATGCTGGAGT GGCAGTTCCA GAAACGACTG CAGGAGTCCA AGCAGAGCGA CGAAGACGAG 
GAGGAGGAGG ACGACGAGGA CGTGGACACC ATGATGATCA TGCAGAGGCT GGAGGCCGAG 
AAGAGAGCCA GGCAGCAGAA TGCTGTCCCA GCTATCTCTG TTCTAGACTT GTTGCAGGAC 
GAGGAGCGGC GGCGTAAGCA GCAGCTGGAG GAGATTCGTA AGCGGGAGGC GGAGGAGCGA 
GCAAAGCAGG AGGAGGAGAG GAGGTGGAGG GAGGAGGAGA GAGCCAAGCG AGAGGCCGAG 
GAGAAGGATT TAACTCGTAC ACTGGAAACT CAGCAGGAGT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027140433.1
CDS	234..5378
Translation	MSGSREEERRKLADIINHWNANRLDLFEISRPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERQLDLDEKPLVVQLNWNKDDREGRFVLKNENDILPKKSQSNGPEKEKDGVIQNFKRTLSKKEKKKEKKREKEFARIPDGDEQALSREDGENSRLAAEVYKDMPENSFTRTISNPEVVMKRRRQQKLEKRMQEFMSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTRDTADFAVVEALEKYGLEKENPREYCIARQDDKSGKEAILDDTECPLQIFRDWPADRGALVFQLKKRPPDYQGRKGRKVDDKGLRGKDGSSSLPPEKLPYLVELSPGRGNHYAYYAYRHHEDGSDSRDKPKLYRLQHSITEVGSDCTEDGAIQLLGPGILPHHCNLMHSEGMVTVTPHGPDADTFVDGQRVTETTVLRSGSTLQFGQAHVFKFVDPMFDQGGKREPAAMMRSRHKSGSVPETTFDLHGDVHSGAALPTSKSSGKLEMERGMVKPMIRGEQQDGRSQDISADRAELTLPAGIEFRDNSEDTFLSAIINYTNSSTVHFKLSPTYVLYMACRYILSPSYRPDMSPSERTHKVIAIVNKMVSMMEGVIQEIPEGDQKQKNIAGALAFWMANASELLNFIKQDRDLNRITLDAQDILAHLVQMAFKYLVHCLQADLNNYMPAFLDDPEEHNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVTDTESGLCCHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVPNIHNTCFKLNSLQLHALMNNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRSLPNGLQDFLEPLLQRGFCRLVPHPRSPGTWTVHFEGADCDSHFSAADNSEMPMRKEPEVVTVTLKKHNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRDKNGKLRPKSEGFELYNSSVQNGSPESPQAGWDSYPEPKKMSGEDRLLKNRADHRSSPNVANQGQSPAGKAVYPGGPGTKITSVSTGNLCADDEPSPPRPEAYPIPTQTYPREYFTIPASKSQDRVVGPGQGQHWQGMEDRERLPVVDNIHNSMQRVNHSQEELYPPPGGLRPEERMQQHYQQDYQHRDMDYQHRELDYQRGGPPGVGGPDHWTPQPQVSSSLESSTSSQEHLNFSASSSSSNKTQNQKTGPGRWKTPNAPHSVPPHSAQPPSRSDLPPPPPPPPATYPESYDPYDPQPDLPLPPPPSAVTPAVAQQAADRKKREEQQRWYEKEKARLEEERERKRREQERKLGQIRANPVMPVQPHNNGGSMPPPHHQPPLPSAAPPQPYLPPAQPQPPPSRPEKLTSLPRSAVPPPASASNPAGMDTVIRELLPQQQPRTIERRDLQYITISKEELTSSDSLSPDPWKRDAREKAEKQQQLHIVDLLDKEIQELQAKPERTAEESDRLRKLMLEWQFQKRLQESKQSDEDEEEEDDEDVDTMMIMQRLEAEKRARQQNAVPAISVLDLLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERAKREAEEKDLTRTLETQQEL

Target ORF information:

RefSeq Version	XM_027284632.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027284632.1

ORF Insert Sequence:

ATGTCGGGCA GCAGGGAGGA GGAGAGGAGG AAACTGGCCG ACATCATCAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CGGCCCACAG AGGACCTGGA GTTCCATGGC 
GTGATGCGGT TCTACTTCCA GGACCGGGTC GCCGGTAACT TTGCCACCAA ATGCATCAGA 
GTGTCGAGCA CGGCGACAAC GCAGGACGTC ATCGAGACGT TAGCAGAGAA GTTCAGACCG 
GACATGAGGA TGCTCTCCTC GCCAAAATAC TCTTTGTACG AGGTCCACGT CAGTGGCGAA 
GAGCGTCAGC TGGATCTGGA CGAGAAGCCT CTGGTGGTTC AGCTGAACTG GAACAAAGAC 
GACAGAGAAG GACGCTTCGT CCTGAAGAAC GAGAACGACA TCCTCCCCAA GAAGAGTCAG 
AGTAACGGCC CGGAGAAGGA GAAGGACGGA GTGATCCAGA ACTTCAAGAG GACTTTGTCC 
AAGAAGGAGA AGAAGAAGGA GAAGAAGAGG GAGAAGGAGT TCGCCCGGAT CCCCGACGGA 
GACGAGCAGG CGCTCAGCCG AGAGGACGGG GAGAACAGTC GTCTGGCGGC GGAGGTTTAC 
AAGGACATGC CGGAGAACAG CTTCACCAGG ACGATCTCCA ACCCGGAGGT GGTGATGAAG 
AGGAGACGCC AGCAGAAGCT GGAGAAACGC ATGCAGGAGT TCATGAGCAG CGACGGGAGG 
CCCGACTCAG GGGGCACGTT GCGGATATAC GCCGACAGCC TGAAGCCGAA CATCCCGTAT 
AAAACCATCC TGCTGTCCAC GAGAGACACG GCTGACTTTG CGGTGGTGGA GGCGCTGGAG 
AAGTACGGGC TGGAGAAGGA GAACCCCCGA GAGTACTGCA TCGCCCGGCA AGACGATAAG 
TCCGGCAAGG AGGCGATCCT GGACGATACC GAGTGTCCAC TGCAGATCTT CAGGGACTGG 
CCTGCAGACA GAGGAGCGCT AGTGTTCCAG CTGAAGAAGA GACCCCCGGA CTACCAGGGC 
AGGAAGGGGA GGAAAGTGGA CGACAAGGGC CTGCGAGGGA AGGACGGCAG CAGCTCCCTG 
CCGCCCGAGA AACTGCCTTA CCTGGTGGAG CTGAGCCCAG GGCGAGGGAA TCACTATGCC 
TACTACGCCT ATCGGCACCA CGAAGACGGG TCTGACTCAC GGGACAAACC AAAGCTGTAC 
CGGCTCCAGC ACAGCATCAC TGAGGTCGGC TCAGACTGCA CAGAGGACGG AGCCATTCAG 
CTGCTCGGTC CGGGGATTCT GCCCCACCAC TGCAACCTCA TGCACAGTGA AGGCATGGTG 
ACGGTGACGC CGCACGGCCC CGACGCCGAC ACCTTCGTCG ACGGGCAACG GGTCACTGAG 
ACGACGGTGC TGCGCTCGGG CTCCACCCTC CAGTTTGGCC AAGCGCACGT CTTCAAGTTT 
GTGGACCCGA TGTTCGACCA GGGAGGGAAG AGAGAGCCGG CCGCCATGAT GAGGAGTCGA 
CACAAGTCCG GCAGCGTACC CGAAACCACC TTCGACCTTC ACGGAGACGT CCACAGCGGA 
GCCGCGCTGC CTACCAGCAA GAGCTCAGGA AAGCTGGAGA TGGAGAGAGG AATGGTCAAA 
CCGATGATCA GAGGAGAGCA GCAGGACGGC AGGTCTCAGG ACATTTCAGC AGACAGAGCC 
GAGTTGACTC TGCCAGCCGG CATCGAGTTC AGAGACAACT CTGAAGACAC CTTCCTGTCG 
GCCATCATCA ACTACACCAA CAGCTCCACG GTTCACTTCA AACTCTCGCC CACGTACGTC 
CTGTACATGG CCTGCCGCTA CATCCTGTCG CCCTCGTACC GGCCCGACAT GTCGCCGTCC 
GAGAGGACGC ACAAGGTCAT CGCCATCGTC AACAAGATGG TGAGCATGAT GGAGGGAGTC 
ATCCAGGAGA TTCCTGAAGG GGATCAGAAG CAGAAGAACA TCGCGGGAGC GCTGGCTTTC 
TGGATGGCCA ACGCCTCCGA GCTGCTCAAC TTCATCAAGC AGGACCGAGA CCTGAACCGC 
ATCACGCTGG ACGCCCAGGA CATCCTCGCC CATCTGGTTC AGATGGCTTT CAAGTACCTG 
GTCCACTGTC TGCAGGCTGA TCTGAATAAC TACATGCCGG CCTTCCTGGA CGACCCCGAG 
GAACACAACC CACAGAGACC CAAGATCGAG GACGTCCTGC ACACCCTCAC GGGGGCGATG 
TCGTTACTGC GGCGGTGTCG GGTCAACGCC GCTCTCACCA TCCAGCTCTT CTCGCAGCTC 
TTCCACTTCA TCAACATGTG GCTCTTCAAC AAGCTGGTGA CGGACACCGA GTCCGGGCTG 
TGCTGTCACT ACTGGGGGGC CATCCTGCGG CAGCAGCTCA GCCACATCGA GGCCTGGGCG 
GAGAAACAGG GGCTGGAGCT CGCCGCCGAC TGCCACCTCA GTCGAATCGT GCAGGCCACC 
ACCCTGCTGA CCATGGACAA ATACTCCATG CAGGATGTGC CGAACATCCA CAACACCTGC 
TTCAAGCTCA ACTCGCTGCA GCTCCACGCC CTCATGAACA ACTACCACTG CGCTCCGGAC 
GAGCCGTACA TCCCGCCGGA GCTGATAGAC CACGTGGTGG CAGTGGCAGA GAACACAGCA 
GATGAGCTGG CGAGGAGCGA CGGCAGAGAG GTTCAGCTGG AGGAGGATCC GGACCTCCAG 
CTGCCGTTCC TCCTTCCCGA GGACGGTTAC TCCTGCGACG TGGTGCGCAG CCTCCCCAAC 
GGCCTGCAGG ACTTCCTGGA GCCGCTGCTG CAGAGAGGTT TCTGTCGGTT GGTACCTCAC 
CCTCGCTCTC CCGGTACCTG GACGGTCCAT TTTGAAGGAG CTGACTGCGA CAGCCACTTC 
TCTGCTGCTG ACAACTCAGA GATGCCAATG AGGAAAGAGC CGGAGGTTGT CACGGTAACC 
CTGAAGAAGC ACAACGGCAT GGGCCTCAGC ATCGTGGCTG CCAAGGGTGC TGGTCAGGAG 
AAGCTGGGCA TCTACATCAA GTCTGTCGTC AAAGGAGGAG CAGCTGATGT GGACGGGCGT 
CTGGCTGCAG GTGACCAGCT GTTGAGCGTG GACGGGCGGA GTCTGGTCGG CCTATCACAA 
GAGAGGGCAG CGGAGCTGAT GACGAGGACG GGCTCAGTCG TGACTCTGGA GGTCGCCAAG 
CAGGGAGCCA TTTACCACGG ACTGGCCACG CTCCTCAACC AGCCGAGCCC CATGATGCCA 
CGAGCGTCAG ACAGAGGCCG CGACAAGAAC GGGAAACTCC GACCAAAGAG CGAAGGCTTC 
GAGTTGTACA ACAGCTCGGT GCAGAACGGC TCTCCGGAGA GCCCGCAGGC CGGCTGGGAC 
TCTTACCCCG AACCAAAGAA GATGAGCGGC GAAGATCGAC TCCTGAAGAA CCGAGCCGAC 
CACCGCTCCA GCCCCAACGT CGCCAATCAG GGCCAGAGTC CTGCAGGCAA AGCAGTGTAC 
CCCGGAGGAC CTGGCACCAA GATCACCTCC GTCTCCACCG GCAACCTGTG CGCAGACGAT 
GAGCCCTCCC CTCCACGTCC AGAAGCTTAC CCCATCCCCA CCCAGACCTA CCCCAGGGAG 
TACTTCACCA TCCCGGCTTC CAAGAGCCAG GATCGGGTCG TTGGTCCTGG GCAGGGACAG 
CACTGGCAGG GCATGGAGGA CAGAGAACGC CTCCCTGTGG TCGACAACAT CCACAACAGC 
ATGCAGCGGG TTAACCACTC CCAGGAGGAG TTGTACCCTC CACCCGGAGG CCTGAGGCCG 
GAGGAGCGCA TGCAGCAGCA CTACCAGCAG GACTACCAGC ACCGCGACAT GGACTACCAG 
CACAGAGAGC TCGACTACCA GAGAGGAGGT CCACCAGGTG TCGGAGGTCC CGATCACTGG 
ACTCCACAGC CTCAGGTGTC CTCCTCGCTG GAGTCGTCCA CGTCCAGCCA GGAGCACCTC 
AACTTCTCCG CCTCCTCCTC GTCCTCCAAC AAAACCCAGA ACCAGAAGAC CGGGCCGGGC 
CGGTGGAAGA CTCCCAACGC CCCTCACTCC GTGCCGCCAC ATTCAGCCCA GCCGCCCTCC 
CGCTCCGACC TGCCCCCTCC TCCGCCTCCA CCGCCAGCTA CTTACCCCGA ATCCTACGAC 
CCATACGACC CCCAACCGGA CCTGCCGCTC CCGCCGCCTC CCTCCGCCGT CACTCCTGCG 
GTAGCTCAGC AGGCGGCCGA CCGCAAGAAA CGTGAGGAGC AGCAGCGCTG GTATGAGAAG 
GAGAAAGCTC GGCTGGAGGA GGAGAGGGAG AGGAAGAGGA GAGAGCAGGA GAGGAAGCTG 
GGTCAGATCC GGGCCAACCC CGTCATGCCC GTCCAGCCTC ACAACAACGG AGGATCCATG 
CCTCCGCCTC ACCACCAGCC GCCTCTGCCG TCTGCAGCCC CGCCTCAGCC CTACCTTCCC 
CCCGCTCAGC CCCAGCCTCC ACCATCCAGA CCGGAGAAGC TGACCAGCCT GCCGCGGTCA 
GCCGTTCCTC CACCCGCCAG CGCCTCCAAC CCTGCAGGCA TGGACACGGT GATCAGGGAG 
CTGTTGCCAC AGCAGCAGCC GCGGACCATC GAGAGGCGAG ACCTGCAGTA CATCACCATC 
AGCAAGGAGG AGCTCACCTC CAGCGACAGT CTGTCTCCAG ACCCCTGGAA GCGAGACGCC 
CGCGAGAAGG CCGAGAAGCA GCAGCAGCTC CACATCGTCG ACCTGCTGGA CAAGGAGATC 
CAGGAGCTCC AGGCGAAGCC GGAGCGGACG GCGGAGGAGA GCGACCGCCT CAGGAAGCTG 
ATGCTGGAGT GGCAGTTCCA GAAACGACTG CAGGAGTCCA AGCAGAGCGA CGAAGACGAG 
GAGGAGGAGG ACGACGAGGA CGTGGACACC ATGATGATCA TGCAGAGGCT GGAGGCCGAG 
AAGAGAGCCA GGCAGCAGAA TGCTGTCCCA GCTATCTCTG TTCTAGACTT GTTGCAGGAC 
GAGGAGCGGC GGCGTAAGCA GCAGCTGGAG GAGATTCGTA AGCGGGAGGC GGAGGAGCGA 
GCAAAGCAGG AGGAGGAGAG GAGGTGGAGG GAGGAGGAGA GAGCCAAGCG AGAGGCCGAG 
GAGAAGGATT TAACTCGTAC ACTGGAAACT CAGCAGGAGT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afdn ( XM_027284633.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_027140434.1 Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X13, mRNA.

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CloneID	OLa200715
Clone ID Related Accession (Same CDS sequence)	XM_027284633.1
Accession Version	XM_027284633.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	afadin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040021.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGCA GCAGGGAGGA GGAGAGGAGG AAACTGGCCG ACATCATCAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CGGCCCACAG AGGACCTGGA GTTCCATGGC 
GTGATGCGGT TCTACTTCCA GGACCGGGTC GCCGGTAACT TTGCCACCAA ATGCATCAGA 
GTGTCGAGCA CGGCGACAAC GCAGGACGTC ATCGAGACGT TAGCAGAGAA GTTCAGACCG 
GACATGAGGA TGCTCTCCTC GCCAAAATAC TCTTTGTACG AGGTCCACGT CAGTGGCGAG 
CGTCAGCTGG ATCTGGACGA GAAGCCTCTG GTGGTTCAGC TGAACTGGAA CAAAGACGAC 
AGAGAAGGAC GCTTCGTCCT GAAGAACGAG AACGACATCC TCCCCAAGAA GAGTCAGAGT 
AACGGCCCGG AGAAGGAGAA GGACGGAGTG ATCCAGAACT TCAAGAGGAC TTTGTCCAAG 
AAGGAGAAGA AGAAGGAGAA GAAGAGGGAG AAGGAGTTCG CCCGGATCCC CGACGGAGAC 
GAGCAGGCGC TCAGCCGAGA GGACGGGGAG AACAGTCGTC TGGCGGCGGA GGTTTACAAG 
GACATGCCGG AGAACAGCTT CACCAGGACG ATCTCCAACC CGGAGGTGGT GATGAAGAGG 
AGACGCCAGC AGAAGCTGGA GAAACGCATG CAGGAGTTCA TGAGCAGCGA CGGGAGGCCC 
GACTCAGGGG GCACGTTGCG GATATACGCC GACAGCCTGA AGCCGAACAT CCCGTATAAA 
ACCATCCTGC TGTCCACGAG AGACACGGCT GACTTTGCGG TGGTGGAGGC GCTGGAGAAG 
TACGGGCTGG AGAAGGAGAA CCCCCGAGAG TACTGCATCG CCCGGCAAGA CGATAAGTCC 
GGCAAGGAGG CGATCCTGGA CGATACCGAG TGTCCACTGC AGATCTTCAG GGACTGGCCT 
GCAGACAGAG GAGCGCTAGT GTTCCAGCTG AAGAAGAGAC CCCCGGACTA CCAGGGCAGG 
AAGGGGAGGA AAGTGGACGA CAAGGGCCTG CGAGGGAAGG ACGGCAGCAG CTCCCTGCCG 
CCCGAGAAAC TGCCTTACCT GGTGGAGCTG AGCCCAGGGC GAGGGAATCA CTATGCCTAC 
TACGCCTATC GGCACCACGA AGACGGGTCT GACTCACGGG ACAAACCAAA GCTGTACCGG 
CTCCAGCACA GCATCACTGA GGTCGGCTCA GACTGCACAG AGGACGGAGC CATTCAGCTG 
CTCGGTCCGG GGATTCTGCC CCACCACTGC AACCTCATGC ACAGTGAAGG CATGGTGACG 
GTGACGCCGC ACGGCCCCGA CGCCGACACC TTCGTCGACG GGCAACGGGT CACTGAGACG 
ACGGTGCTGC GCTCGGGCTC CACCCTCCAG TTTGGCCAAG CGCACGTCTT CAAGTTTGTG 
GACCCGATGT TCGACCAGGG AGGGAAGAGA GAGCCGGCCG CCATGATGAG GAGTCGACAC 
AAGTCCGGCA GCGTACCCGA AACCACCTTC GACCTTCACG GAGACGTCCA CAGCGGAGCC 
GCGCTGCCTA CCAGCAAGAG CTCAGGAAAG CTGGAGATGG AGAGAGGAAT GGTCAAACCG 
ATGATCAGAG GAGAGCAGCA GGACGGCAGG TCTCAGGACA TTTCAGCAGA CAGAGCCGAG 
TTGACTCTGC CAGCCGGCAT CGAGTTCAGA GACAACTCTG AAGACACCTT CCTGTCGGCC 
ATCATCAACT ACACCAACAG CTCCACGGTT CACTTCAAAC TCTCGCCCAC GTACGTCCTG 
TACATGGCCT GCCGCTACAT CCTGTCGCCC TCGTACCGGC CCGACATGTC GCCGTCCGAG 
AGGACGCACA AGGTCATCGC CATCGTCAAC AAGATGGTGA GCATGATGGA GGGAGTCATC 
CAGGAGATTC CTGAAGGGGA TCAGAAGCAG AAGAACATCG CGGGAGCGCT GGCTTTCTGG 
ATGGCCAACG CCTCCGAGCT GCTCAACTTC ATCAAGCAGG ACCGAGACCT GAACCGCATC 
ACGCTGGACG CCCAGGACAT CCTCGCCCAT CTGGTTCAGA TGGCTTTCAA GTACCTGGTC 
CACTGTCTGC AGGCTGATCT GAATAACTAC ATGCCGGCCT TCCTGGACGA CCCCGAGGAA 
CACAACCCAC AGAGACCCAA GATCGAGGAC GTCCTGCACA CCCTCACGGG GGCGATGTCG 
TTACTGCGGC GGTGTCGGGT CAACGCCGCT CTCACCATCC AGCTCTTCTC GCAGCTCTTC 
CACTTCATCA ACATGTGGCT CTTCAACAAG CTGGTGACGG ACACCGAGTC CGGGCTGTGC 
TGTCACTACT GGGGGGCCAT CCTGCGGCAG CAGCTCAGCC ACATCGAGGC CTGGGCGGAG 
AAACAGGGGC TGGAGCTCGC CGCCGACTGC CACCTCAGTC GAATCGTGCA GGCCACCACC 
CTGCTGACCA TGGACAAATA CTCCATGCAG GATGTGCCGA ACATCCACAA CACCTGCTTC 
AAGCTCAACT CGCTGCAGCT CCACGCCCTC ATGAACAACT ACCACTGCGC TCCGGACGAG 
CCGTACATCC CGCCGGAGCT GATAGACCAC GTGGTGGCAG TGGCAGAGAA CACAGCAGAT 
GAGCTGGCGA GGAGCGACGG CAGAGAGGTT CAGCTGGAGG AGGATCCGGA CCTCCAGCTG 
CCGTTCCTCC TTCCCGAGGA CGGTTACTCC TGCGACGTGG TGCGCAGCCT CCCCAACGGC 
CTGCAGGACT TCCTGGAGCC GCTGCTGCAG AGAGGTTTCT GTCGGTTGGT ACCTCACCCT 
CGCTCTCCCG GTACCTGGAC GGTCCATTTT GAAGGAGCTG ACTGCGACAG CCACTTCTCT 
GCTGCTGACA ACTCAGAGAT GCCAATGAGG AAAGAGCCGG AGGTTGTCAC GGTAACCCTG 
AAGAAGCACA ACGGCATGGG CCTCAGCATC GTGGCTGCCA AGGGTGCTGG TCAGGAGAAG 
CTGGGCATCT ACATCAAGTC TGTCGTCAAA GGAGGAGCAG CTGATGTGGA CGGGCGTCTG 
GCTGCAGGTG ACCAGCTGTT GAGCGTGGAC GGGCGGAGTC TGGTCGGCCT ATCACAAGAG 
AGGGCAGCGG AGCTGATGAC GAGGACGGGC TCAGTCGTGA CTCTGGAGGT CGCCAAGCAG 
GGAGCCATTT ACCACGGACT GGCCACGCTC CTCAACCAGC CGAGCCCCAT GATGCCACGA 
GCGTCAGACA GAGGCCGCGA CAAGAACGGG AAACTCCGAC CAAAGAGCGA AGGCTTCGAG 
TTGTACAACA GCTCGGTGCA GAACGGCTCT CCGGAGAGCC CGCAGGCCGG CTGGGACTCT 
TACCCCGAAC CAAAGAAGAT GAGCGGCGAA GATCGACTCC TGAAGAACCG AGCCGACCAC 
CGCTCCAGCC CCAACGTCGC CAATCAGGGC CAGAGTCCTG CAGGCAAAGC AGTGTACCCC 
GGAGGACCTG GCACCAAGAT CACCTCCGTC TCCACCGGCA ACCTGTGCGC AGACGATGAG 
CCCTCCCCTC CACGTCCAGA AGCTTACCCC ATCCCCACCC AGACCTACCC CAGGGAGTAC 
TTCACCATCC CGGCTTCCAA GAGCCAGGAT CGGGTCGTTG GTCCTGGGCA GGGACAGCAC 
TGGCAGGGCA TGGAGGACAG AGAACGCCTC CCTGTGGTCG ACAACATCCA CAACAGCATG 
CAGCGGGTTA ACCACTCCCA GGAGGAGTTG TACCCTCCAC CCGGAGGCCT GAGGCCGGAG 
GAGCGCATGC AGCAGCACTA CCAGCAGGAC TACCAGCACC GCGACATGGA CTACCAGCAC 
AGAGAGCTCG ACTACCAGAG AGGAGGTCCA CCAGGTGTCG GAGGTCCCGA TCACTGGACT 
CCACAGCCTC AGGTGTCCTC CTCGCTGGAG TCGTCCACGT CCAGCCAGGA GCACCTCAAC 
TTCTCCGCCT CCTCCTCGTC CTCCAACAAA ACCCAGAACC AGAAGACCGG GCCGGGCCGG 
TGGAAGACTC CCAACGCCCC TCACTCCGTG CCGCCACATT CAGCCCAGCC GCCCTCCCGC 
TCCGACCTGC CCCCTCCTCC GCCTCCACCG CCAGCTACTT ACCCCGAATC CTACGACCCA 
TACGACCCCC AACCGGACCT GCCGCTCCCG CCGCCTCCCT CCGCCGTCAC TCCTGCGGTA 
GCTCAGCAGG CGGCCGACCG CAAGAAACGT GAGGAGCAGC AGCGCTGGTA TGAGAAGGAG 
AAAGCTCGGC TGGAGGAGGA GAGGGAGAGG AAGAGGAGAG AGCAGGAGAG GAAGCTGGGT 
CAGATCCGGG CCAACCCCGT CATGCCCGTC CAGCCTCACA ACAACGGAGG ATCCATGCCT 
CCGCCTCACC ACCAGCCGCC TCTGCCGTCT GCAGCCCCGC CTCAGCCCTA CCTTCCCCCC 
GCTCAGCCCC AGCCTCCACC ATCCAGACCG GAGAAGCTGA CCAGCCTGCC GCGGTCAGCC 
GTTCCTCCAC CCGCCAGCGC CTCCAACCCT GCAGGCATGG ACACGGTGAT CAGGGAGCTG 
TTGCCACAGC AGCAGCCGCG GACCATCGAG AGGCGAGACC TGCAGTACAT CACCATCAGC 
AAGGAGGAGC TCACCTCCAG CGACAGTCTG TCTCCAGACC CCTGGAAGCG AGACGCCCGC 
GAGAAGGCCG AGAAGCAGCA GCAGCTCCAC ATCGTCGACC TGCTGGACAA GGAGATCCAG 
GAGCTCCAGG CGAAGCCGGA GCGGACGGCG GAGGAGAGCG ACCGCCTCAG GAAGCTGATG 
CTGGAGTGGC AGTTCCAGAA ACGACTGCAG GAGTCCAAGC AGAGCGACGA AGACGAGGAG 
GAGGAGGACG ACGAGGACGT GGACACCATG ATGATCATGC AGAGGCTGGA GGCCGAGAAG 
AGAGCCAGGT TGCAGGACGA GGAGCGGCGG CGTAAGCAGC AGCTGGAGGA GATTCGTAAG 
CGGGAGGCGG AGGAGCGAGC AAAGCAGGAG GAGGAGAGGA GGTGGAGGGA GGAGGAGAGA 
GCCAAGCGAG AGGCCGAGGA GAAGAGGAGA CAGGAGGAGG AGTACTACAC TCGCCTGGAG 
GCGGAGCGGC GCCGGCAGCA CGACGAGGCC GAGAGGAAGC TGCTGACGCC CGACGACCCG 
GCCGGTCTGT ATCGGCCCCC GCTGCCCCGG GATTACCAAA CCCCGCCCCC CCAACAAACC 
TATAAAACCC CCCCGCCCCC CCCACAGAGA AACACCTCCT ACCTCAAAAC GCAGGTCACC 
TCCCCCGACA CGCTCTACAC CGCCAAGTTT GTCTCCTACG CGGGCGACGA GGAGGAAGAC 
GAGGAGGCAG GGAGTGGCGG CGGCGGCGGC AGCAGCAGCC AATCAGGTCA GGTGAAGCTG 
TCGGCGACCC GGAAGTCGTA CGGCGACCTC CCGGCCTCCG CCTCGCCCAA TCACAGCAGG 
CCTCAGCCGC CGCCCACTGC GCGCAAGCCC CGCCCTGTTT CTGACGGCAT CTTCCTGTCT 
GGCTCGTTCC AGCCGCCATT CAACAACAAC TCCAACAGTA CAGGTCCCCC CCTTCCTGCC 
AAACCCAGCT CCATCCCCCC GGGAGGCTTT AAAGGATTTA ACTCGTACAC TGGAAACTCA 
GCAGGAGTTG TAGGATCAGG AGAAGTCTAC AAGGACCCGC GAGACAAATG GACGAAGAGT 
CAGGAGCAGG AGAACTCGAA CCCCAGCGGC CCTCCGGAGA ATCTGACCTT CAAGGAGCGG 
CAGCGTCTCT TCTCTCAGGG GAAGGAGGTT TCCAACAAGG TCAAAGCTTC GCGCAAACTC 
ATGGAGCTGG AGAACGAGCT CAACACCAAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027140434.1
CDS	233..6085
Translation	MSGSREEERRKLADIINHWNANRLDLFEISRPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERQLDLDEKPLVVQLNWNKDDREGRFVLKNENDILPKKSQSNGPEKEKDGVIQNFKRTLSKKEKKKEKKREKEFARIPDGDEQALSREDGENSRLAAEVYKDMPENSFTRTISNPEVVMKRRRQQKLEKRMQEFMSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTRDTADFAVVEALEKYGLEKENPREYCIARQDDKSGKEAILDDTECPLQIFRDWPADRGALVFQLKKRPPDYQGRKGRKVDDKGLRGKDGSSSLPPEKLPYLVELSPGRGNHYAYYAYRHHEDGSDSRDKPKLYRLQHSITEVGSDCTEDGAIQLLGPGILPHHCNLMHSEGMVTVTPHGPDADTFVDGQRVTETTVLRSGSTLQFGQAHVFKFVDPMFDQGGKREPAAMMRSRHKSGSVPETTFDLHGDVHSGAALPTSKSSGKLEMERGMVKPMIRGEQQDGRSQDISADRAELTLPAGIEFRDNSEDTFLSAIINYTNSSTVHFKLSPTYVLYMACRYILSPSYRPDMSPSERTHKVIAIVNKMVSMMEGVIQEIPEGDQKQKNIAGALAFWMANASELLNFIKQDRDLNRITLDAQDILAHLVQMAFKYLVHCLQADLNNYMPAFLDDPEEHNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVTDTESGLCCHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVPNIHNTCFKLNSLQLHALMNNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRSLPNGLQDFLEPLLQRGFCRLVPHPRSPGTWTVHFEGADCDSHFSAADNSEMPMRKEPEVVTVTLKKHNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRDKNGKLRPKSEGFELYNSSVQNGSPESPQAGWDSYPEPKKMSGEDRLLKNRADHRSSPNVANQGQSPAGKAVYPGGPGTKITSVSTGNLCADDEPSPPRPEAYPIPTQTYPREYFTIPASKSQDRVVGPGQGQHWQGMEDRERLPVVDNIHNSMQRVNHSQEELYPPPGGLRPEERMQQHYQQDYQHRDMDYQHRELDYQRGGPPGVGGPDHWTPQPQVSSSLESSTSSQEHLNFSASSSSSNKTQNQKTGPGRWKTPNAPHSVPPHSAQPPSRSDLPPPPPPPPATYPESYDPYDPQPDLPLPPPPSAVTPAVAQQAADRKKREEQQRWYEKEKARLEEERERKRREQERKLGQIRANPVMPVQPHNNGGSMPPPHHQPPLPSAAPPQPYLPPAQPQPPPSRPEKLTSLPRSAVPPPASASNPAGMDTVIRELLPQQQPRTIERRDLQYITISKEELTSSDSLSPDPWKRDAREKAEKQQQLHIVDLLDKEIQELQAKPERTAEESDRLRKLMLEWQFQKRLQESKQSDEDEEEEDDEDVDTMMIMQRLEAEKRARLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERAKREAEEKRRQEEEYYTRLEAERRRQHDEAERKLLTPDDPAGLYRPPLPRDYQTPPPQQTYKTPPPPPQRNTSYLKTQVTSPDTLYTAKFVSYAGDEEEDEEAGSGGGGGSSSQSGQVKLSATRKSYGDLPASASPNHSRPQPPPTARKPRPVSDGIFLSGSFQPPFNNNSNSTGPPLPAKPSSIPPGGFKGFNSYTGNSAGVVGSGEVYKDPRDKWTKSQEQENSNPSGPPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNTK

Target ORF information:

RefSeq Version	XM_027284633.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027284633.1

ORF Insert Sequence:

ATGTCGGGCA GCAGGGAGGA GGAGAGGAGG AAACTGGCCG ACATCATCAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CGGCCCACAG AGGACCTGGA GTTCCATGGC 
GTGATGCGGT TCTACTTCCA GGACCGGGTC GCCGGTAACT TTGCCACCAA ATGCATCAGA 
GTGTCGAGCA CGGCGACAAC GCAGGACGTC ATCGAGACGT TAGCAGAGAA GTTCAGACCG 
GACATGAGGA TGCTCTCCTC GCCAAAATAC TCTTTGTACG AGGTCCACGT CAGTGGCGAG 
CGTCAGCTGG ATCTGGACGA GAAGCCTCTG GTGGTTCAGC TGAACTGGAA CAAAGACGAC 
AGAGAAGGAC GCTTCGTCCT GAAGAACGAG AACGACATCC TCCCCAAGAA GAGTCAGAGT 
AACGGCCCGG AGAAGGAGAA GGACGGAGTG ATCCAGAACT TCAAGAGGAC TTTGTCCAAG 
AAGGAGAAGA AGAAGGAGAA GAAGAGGGAG AAGGAGTTCG CCCGGATCCC CGACGGAGAC 
GAGCAGGCGC TCAGCCGAGA GGACGGGGAG AACAGTCGTC TGGCGGCGGA GGTTTACAAG 
GACATGCCGG AGAACAGCTT CACCAGGACG ATCTCCAACC CGGAGGTGGT GATGAAGAGG 
AGACGCCAGC AGAAGCTGGA GAAACGCATG CAGGAGTTCA TGAGCAGCGA CGGGAGGCCC 
GACTCAGGGG GCACGTTGCG GATATACGCC GACAGCCTGA AGCCGAACAT CCCGTATAAA 
ACCATCCTGC TGTCCACGAG AGACACGGCT GACTTTGCGG TGGTGGAGGC GCTGGAGAAG 
TACGGGCTGG AGAAGGAGAA CCCCCGAGAG TACTGCATCG CCCGGCAAGA CGATAAGTCC 
GGCAAGGAGG CGATCCTGGA CGATACCGAG TGTCCACTGC AGATCTTCAG GGACTGGCCT 
GCAGACAGAG GAGCGCTAGT GTTCCAGCTG AAGAAGAGAC CCCCGGACTA CCAGGGCAGG 
AAGGGGAGGA AAGTGGACGA CAAGGGCCTG CGAGGGAAGG ACGGCAGCAG CTCCCTGCCG 
CCCGAGAAAC TGCCTTACCT GGTGGAGCTG AGCCCAGGGC GAGGGAATCA CTATGCCTAC 
TACGCCTATC GGCACCACGA AGACGGGTCT GACTCACGGG ACAAACCAAA GCTGTACCGG 
CTCCAGCACA GCATCACTGA GGTCGGCTCA GACTGCACAG AGGACGGAGC CATTCAGCTG 
CTCGGTCCGG GGATTCTGCC CCACCACTGC AACCTCATGC ACAGTGAAGG CATGGTGACG 
GTGACGCCGC ACGGCCCCGA CGCCGACACC TTCGTCGACG GGCAACGGGT CACTGAGACG 
ACGGTGCTGC GCTCGGGCTC CACCCTCCAG TTTGGCCAAG CGCACGTCTT CAAGTTTGTG 
GACCCGATGT TCGACCAGGG AGGGAAGAGA GAGCCGGCCG CCATGATGAG GAGTCGACAC 
AAGTCCGGCA GCGTACCCGA AACCACCTTC GACCTTCACG GAGACGTCCA CAGCGGAGCC 
GCGCTGCCTA CCAGCAAGAG CTCAGGAAAG CTGGAGATGG AGAGAGGAAT GGTCAAACCG 
ATGATCAGAG GAGAGCAGCA GGACGGCAGG TCTCAGGACA TTTCAGCAGA CAGAGCCGAG 
TTGACTCTGC CAGCCGGCAT CGAGTTCAGA GACAACTCTG AAGACACCTT CCTGTCGGCC 
ATCATCAACT ACACCAACAG CTCCACGGTT CACTTCAAAC TCTCGCCCAC GTACGTCCTG 
TACATGGCCT GCCGCTACAT CCTGTCGCCC TCGTACCGGC CCGACATGTC GCCGTCCGAG 
AGGACGCACA AGGTCATCGC CATCGTCAAC AAGATGGTGA GCATGATGGA GGGAGTCATC 
CAGGAGATTC CTGAAGGGGA TCAGAAGCAG AAGAACATCG CGGGAGCGCT GGCTTTCTGG 
ATGGCCAACG CCTCCGAGCT GCTCAACTTC ATCAAGCAGG ACCGAGACCT GAACCGCATC 
ACGCTGGACG CCCAGGACAT CCTCGCCCAT CTGGTTCAGA TGGCTTTCAA GTACCTGGTC 
CACTGTCTGC AGGCTGATCT GAATAACTAC ATGCCGGCCT TCCTGGACGA CCCCGAGGAA 
CACAACCCAC AGAGACCCAA GATCGAGGAC GTCCTGCACA CCCTCACGGG GGCGATGTCG 
TTACTGCGGC GGTGTCGGGT CAACGCCGCT CTCACCATCC AGCTCTTCTC GCAGCTCTTC 
CACTTCATCA ACATGTGGCT CTTCAACAAG CTGGTGACGG ACACCGAGTC CGGGCTGTGC 
TGTCACTACT GGGGGGCCAT CCTGCGGCAG CAGCTCAGCC ACATCGAGGC CTGGGCGGAG 
AAACAGGGGC TGGAGCTCGC CGCCGACTGC CACCTCAGTC GAATCGTGCA GGCCACCACC 
CTGCTGACCA TGGACAAATA CTCCATGCAG GATGTGCCGA ACATCCACAA CACCTGCTTC 
AAGCTCAACT CGCTGCAGCT CCACGCCCTC ATGAACAACT ACCACTGCGC TCCGGACGAG 
CCGTACATCC CGCCGGAGCT GATAGACCAC GTGGTGGCAG TGGCAGAGAA CACAGCAGAT 
GAGCTGGCGA GGAGCGACGG CAGAGAGGTT CAGCTGGAGG AGGATCCGGA CCTCCAGCTG 
CCGTTCCTCC TTCCCGAGGA CGGTTACTCC TGCGACGTGG TGCGCAGCCT CCCCAACGGC 
CTGCAGGACT TCCTGGAGCC GCTGCTGCAG AGAGGTTTCT GTCGGTTGGT ACCTCACCCT 
CGCTCTCCCG GTACCTGGAC GGTCCATTTT GAAGGAGCTG ACTGCGACAG CCACTTCTCT 
GCTGCTGACA ACTCAGAGAT GCCAATGAGG AAAGAGCCGG AGGTTGTCAC GGTAACCCTG 
AAGAAGCACA ACGGCATGGG CCTCAGCATC GTGGCTGCCA AGGGTGCTGG TCAGGAGAAG 
CTGGGCATCT ACATCAAGTC TGTCGTCAAA GGAGGAGCAG CTGATGTGGA CGGGCGTCTG 
GCTGCAGGTG ACCAGCTGTT GAGCGTGGAC GGGCGGAGTC TGGTCGGCCT ATCACAAGAG 
AGGGCAGCGG AGCTGATGAC GAGGACGGGC TCAGTCGTGA CTCTGGAGGT CGCCAAGCAG 
GGAGCCATTT ACCACGGACT GGCCACGCTC CTCAACCAGC CGAGCCCCAT GATGCCACGA 
GCGTCAGACA GAGGCCGCGA CAAGAACGGG AAACTCCGAC CAAAGAGCGA AGGCTTCGAG 
TTGTACAACA GCTCGGTGCA GAACGGCTCT CCGGAGAGCC CGCAGGCCGG CTGGGACTCT 
TACCCCGAAC CAAAGAAGAT GAGCGGCGAA GATCGACTCC TGAAGAACCG AGCCGACCAC 
CGCTCCAGCC CCAACGTCGC CAATCAGGGC CAGAGTCCTG CAGGCAAAGC AGTGTACCCC 
GGAGGACCTG GCACCAAGAT CACCTCCGTC TCCACCGGCA ACCTGTGCGC AGACGATGAG 
CCCTCCCCTC CACGTCCAGA AGCTTACCCC ATCCCCACCC AGACCTACCC CAGGGAGTAC 
TTCACCATCC CGGCTTCCAA GAGCCAGGAT CGGGTCGTTG GTCCTGGGCA GGGACAGCAC 
TGGCAGGGCA TGGAGGACAG AGAACGCCTC CCTGTGGTCG ACAACATCCA CAACAGCATG 
CAGCGGGTTA ACCACTCCCA GGAGGAGTTG TACCCTCCAC CCGGAGGCCT GAGGCCGGAG 
GAGCGCATGC AGCAGCACTA CCAGCAGGAC TACCAGCACC GCGACATGGA CTACCAGCAC 
AGAGAGCTCG ACTACCAGAG AGGAGGTCCA CCAGGTGTCG GAGGTCCCGA TCACTGGACT 
CCACAGCCTC AGGTGTCCTC CTCGCTGGAG TCGTCCACGT CCAGCCAGGA GCACCTCAAC 
TTCTCCGCCT CCTCCTCGTC CTCCAACAAA ACCCAGAACC AGAAGACCGG GCCGGGCCGG 
TGGAAGACTC CCAACGCCCC TCACTCCGTG CCGCCACATT CAGCCCAGCC GCCCTCCCGC 
TCCGACCTGC CCCCTCCTCC GCCTCCACCG CCAGCTACTT ACCCCGAATC CTACGACCCA 
TACGACCCCC AACCGGACCT GCCGCTCCCG CCGCCTCCCT CCGCCGTCAC TCCTGCGGTA 
GCTCAGCAGG CGGCCGACCG CAAGAAACGT GAGGAGCAGC AGCGCTGGTA TGAGAAGGAG 
AAAGCTCGGC TGGAGGAGGA GAGGGAGAGG AAGAGGAGAG AGCAGGAGAG GAAGCTGGGT 
CAGATCCGGG CCAACCCCGT CATGCCCGTC CAGCCTCACA ACAACGGAGG ATCCATGCCT 
CCGCCTCACC ACCAGCCGCC TCTGCCGTCT GCAGCCCCGC CTCAGCCCTA CCTTCCCCCC 
GCTCAGCCCC AGCCTCCACC ATCCAGACCG GAGAAGCTGA CCAGCCTGCC GCGGTCAGCC 
GTTCCTCCAC CCGCCAGCGC CTCCAACCCT GCAGGCATGG ACACGGTGAT CAGGGAGCTG 
TTGCCACAGC AGCAGCCGCG GACCATCGAG AGGCGAGACC TGCAGTACAT CACCATCAGC 
AAGGAGGAGC TCACCTCCAG CGACAGTCTG TCTCCAGACC CCTGGAAGCG AGACGCCCGC 
GAGAAGGCCG AGAAGCAGCA GCAGCTCCAC ATCGTCGACC TGCTGGACAA GGAGATCCAG 
GAGCTCCAGG CGAAGCCGGA GCGGACGGCG GAGGAGAGCG ACCGCCTCAG GAAGCTGATG 
CTGGAGTGGC AGTTCCAGAA ACGACTGCAG GAGTCCAAGC AGAGCGACGA AGACGAGGAG 
GAGGAGGACG ACGAGGACGT GGACACCATG ATGATCATGC AGAGGCTGGA GGCCGAGAAG 
AGAGCCAGGT TGCAGGACGA GGAGCGGCGG CGTAAGCAGC AGCTGGAGGA GATTCGTAAG 
CGGGAGGCGG AGGAGCGAGC AAAGCAGGAG GAGGAGAGGA GGTGGAGGGA GGAGGAGAGA 
GCCAAGCGAG AGGCCGAGGA GAAGAGGAGA CAGGAGGAGG AGTACTACAC TCGCCTGGAG 
GCGGAGCGGC GCCGGCAGCA CGACGAGGCC GAGAGGAAGC TGCTGACGCC CGACGACCCG 
GCCGGTCTGT ATCGGCCCCC GCTGCCCCGG GATTACCAAA CCCCGCCCCC CCAACAAACC 
TATAAAACCC CCCCGCCCCC CCCACAGAGA AACACCTCCT ACCTCAAAAC GCAGGTCACC 
TCCCCCGACA CGCTCTACAC CGCCAAGTTT GTCTCCTACG CGGGCGACGA GGAGGAAGAC 
GAGGAGGCAG GGAGTGGCGG CGGCGGCGGC AGCAGCAGCC AATCAGGTCA GGTGAAGCTG 
TCGGCGACCC GGAAGTCGTA CGGCGACCTC CCGGCCTCCG CCTCGCCCAA TCACAGCAGG 
CCTCAGCCGC CGCCCACTGC GCGCAAGCCC CGCCCTGTTT CTGACGGCAT CTTCCTGTCT 
GGCTCGTTCC AGCCGCCATT CAACAACAAC TCCAACAGTA CAGGTCCCCC CCTTCCTGCC 
AAACCCAGCT CCATCCCCCC GGGAGGCTTT AAAGGATTTA ACTCGTACAC TGGAAACTCA 
GCAGGAGTTG TAGGATCAGG AGAAGTCTAC AAGGACCCGC GAGACAAATG GACGAAGAGT 
CAGGAGCAGG AGAACTCGAA CCCCAGCGGC CCTCCGGAGA ATCTGACCTT CAAGGAGCGG 
CAGCGTCTCT TCTCTCAGGG GAAGGAGGTT TCCAACAAGG TCAAAGCTTC GCGCAAACTC 
ATGGAGCTGG AGAACGAGCT CAACACCAAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afdn ( XM_027284634.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_027140435.1 Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X14, mRNA.

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CloneID	OLa200716
Clone ID Related Accession (Same CDS sequence)	XM_027284634.1
Accession Version	XM_027284634.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	afadin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040021.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGCA GCAGGGAGGA GGAGAGGAGG AAACTGGCCG ACATCATCAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CGGCCCACAG AGGACCTGGA GTTCCATGGC 
GTGATGCGGT TCTACTTCCA GGACCGGGTC GCCGGTAACT TTGCCACCAA ATGCATCAGA 
GTGTCGAGCA CGGCGACAAC GCAGGACGTC ATCGAGACGT TAGCAGAGAA GTTCAGACCG 
GACATGAGGA TGCTCTCCTC GCCAAAATAC TCTTTGTACG AGGTCCACGT CAGTGGCGAG 
CGTCAGCTGG ATCTGGACGA GAAGCCTCTG GTGGTTCAGC TGAACTGGAA CAAAGACGAC 
AGAGAAGGAC GCTTCGTCCT GAAGAACGAG AACGACATCC TCCCCAAGAA GAGTCAGAGT 
AACGGCCCGG AGAAGGAGAA GGACGGAGTG ATCCAGAACT TCAAGAGGAC TTTGTCCAAG 
AAGGAGAAGA AGAAGGAGAA GAAGAGGGAG AAGGAGTTCG CCCGGATCCC CGACGGAGAC 
GAGCAGGCGC TCAGCCGAGA GGACGGGGAG AACAGTCGTC TGGCGGCGGA GGTTTACAAG 
GACATGCCGG AGAACAGCTT CACCAGGACG ATCTCCAACC CGGAGGTGGT GATGAAGAGG 
AGACGCCAGC AGAAGCTGGA GAAACGCATG CAGGAGTTCA TGAGCAGCGA CGGGAGGCCC 
GACTCAGGGG GCACGTTGCG GATATACGCC GACAGCCTGA AGCCGAACAT CCCGTATAAA 
ACCATCCTGC TGTCCACGAG AGACACGGCT GACTTTGCGG TGGTGGAGGC GCTGGAGAAG 
TACGGGCTGG AGAAGGAGAA CCCCCGAGAG TACTGCATCG CCCGGCAAGA CGATAAGTCC 
GGCAAGGAGG CGATCCTGGA CGATACCGAG TGTCCACTGC AGATCTTCAG GGACTGGCCT 
GCAGACAGAG GAGCGCTAGT GTTCCAGCTG AAGAAGAGAC CCCCGGACTA CCAGGGCAGG 
AAGGGGAGGA AAGTGGACGA CAAGGGCCTG CGAGGGAAGG ACGGCAGCAG CTCCCTGCCG 
CCCGAGAAAC TGCCTTACCT GGTGGAGCTG AGCCCAGGGC GAGGGAATCA CTATGCCTAC 
TACGCCTATC GGCACCACGA AGACGGGTCT GACTCACGGG ACAAACCAAA GCTGTACCGG 
CTCCAGCACA GCATCACTGA GGTCGGCTCA GACTGCACAG AGGACGGAGC CATTCAGCTG 
CTCGGTCCGG GGATTCTGCC CCACCACTGC AACCTCATGC ACAGTGAAGG CATGGTGACG 
GTGACGCCGC ACGGCCCCGA CGCCGACACC TTCGTCGACG GGCAACGGGT CACTGAGACG 
ACGGTGCTGC GCTCGGGCTC CACCCTCCAG TTTGGCCAAG CGCACGTCTT CAAGTTTGTG 
GACCCGATGT TCGACCAGGG AGGGAAGAGA GAGCCGGCCG CCATGATGAG GAGTCGACAC 
AAGTCCGGCA GCGTACCCGA AACCACCTTC GACCTTCACG GAGACGTCCA CAGCGGAGCC 
GCGCTGCCTA CCAGCAAGAG CTCAGGAAAG CTGGAGATGG AGAGAGGAAT GGTCAAACCG 
ATGATCAGAG GAGAGCAGCA GGACGGCAGG TCTCAGGACA TTTCAGCAGA CAGAGCCGAG 
TTGACTCTGC CAGCCGGCAT CGAGTTCAGA GACAACTCTG AAGACACCTT CCTGTCGGCC 
ATCATCAACT ACACCAACAG CTCCACGGTT CACTTCAAAC TCTCGCCCAC GTACGTCCTG 
TACATGGCCT GCCGCTACAT CCTGTCGCCC TCGTACCGGC CCGACATGTC GCCGTCCGAG 
AGGACGCACA AGGTCATCGC CATCGTCAAC AAGATGGTGA GCATGATGGA GGGAGTCATC 
CAGAAGCAGA AGAACATCGC GGGAGCGCTG GCTTTCTGGA TGGCCAACGC CTCCGAGCTG 
CTCAACTTCA TCAAGCAGGA CCGAGACCTG AACCGCATCA CGCTGGACGC CCAGGACATC 
CTCGCCCATC TGGTTCAGAT GGCTTTCAAG TACCTGGTCC ACTGTCTGCA GGCTGATCTG 
AATAACTACA TGCCGGCCTT CCTGGACGAC CCCGAGGAAC ACAACCCACA GAGACCCAAG 
ATCGAGGACG TCCTGCACAC CCTCACGGGG GCGATGTCGT TACTGCGGCG GTGTCGGGTC 
AACGCCGCTC TCACCATCCA GCTCTTCTCG CAGCTCTTCC ACTTCATCAA CATGTGGCTC 
TTCAACAAGC TGGTGACGGA CACCGAGTCC GGGCTGTGCT GTCACTACTG GGGGGCCATC 
CTGCGGCAGC AGCTCAGCCA CATCGAGGCC TGGGCGGAGA AACAGGGGCT GGAGCTCGCC 
GCCGACTGCC ACCTCAGTCG AATCGTGCAG GCCACCACCC TGCTGACCAT GGACAAATAC 
TCCATGCAGG ATGTGCCGAA CATCCACAAC ACCTGCTTCA AGCTCAACTC GCTGCAGCTC 
CACGCCCTCA TGAACAACTA CCACTGCGCT CCGGACGAGC CGTACATCCC GCCGGAGCTG 
ATAGACCACG TGGTGGCAGT GGCAGAGAAC ACAGCAGATG AGCTGGCGAG GAGCGACGGC 
AGAGAGGTTC AGCTGGAGGA GGATCCGGAC CTCCAGCTGC CGTTCCTCCT TCCCGAGGAC 
GGTTACTCCT GCGACGTGGT GCGCAGCCTC CCCAACGGCC TGCAGGACTT CCTGGAGCCG 
CTGCTGCAGA GAGGTTTCTG TCGGTTGGTA CCTCACCCTC GCTCTCCCGG TACCTGGACG 
GTCCATTTTG AAGGAGCTGA CTGCGACAGC CACTTCTCTG CTGCTGACAA CTCAGAGATG 
CCAATGAGGA AAGAGCCGGA GGTTGTCACG GTAACCCTGA AGAAGCACAA CGGCATGGGC 
CTCAGCATCG TGGCTGCCAA GGGTGCTGGT CAGGAGAAGC TGGGCATCTA CATCAAGTCT 
GTCGTCAAAG GAGGAGCAGC TGATGTGGAC GGGCGTCTGG CTGCAGGTGA CCAGCTGTTG 
AGCGTGGACG GGCGGAGTCT GGTCGGCCTA TCACAAGAGA GGGCAGCGGA GCTGATGACG 
AGGACGGGCT CAGTCGTGAC TCTGGAGGTC GCCAAGCAGG GAGCCATTTA CCACGGACTG 
GCCACGCTCC TCAACCAGCC GAGCCCCATG ATGCCACGAG CGTCAGACAG AGGCCGCGAC 
AAGAACGGGA AACTCCGACC AAAGAGCGAA GGCTTCGAGT TGTACAACAG CTCGGTGCAG 
AACGGCTCTC CGGAGAGCCC GCAGGCCGGC TGGGACTCTT ACCCCGAACC AAAGAAGATG 
AGCGGCGAAG ATCGACTCCT GAAGAACCGA GCCGACCACC GCTCCAGCCC CAACGTCGCC 
AATCAGGGCC AGAGTCCTGC AGGCAAAGCA GTGTACCCCG GAGGACCTGG CACCAAGATC 
ACCTCCGTCT CCACCGGCAA CCTGTGCGCA GACGATGAGC CCTCCCCTCC ACGTCCAGAA 
GCTTACCCCA TCCCCACCCA GACCTACCCC AGGGAGTACT TCACCATCCC GGCTTCCAAG 
AGCCAGGATC GGGTCGTTGG TCCTGGGCAG GGACAGCACT GGCAGGGCAT GGAGGACAGA 
GAACGCCTCC CTGTGGTCGA CAACATCCAC AACAGCATGC AGCGGGTTAA CCACTCCCAG 
GAGGAGTTGT ACCCTCCACC CGGAGGCCTG AGGCCGGAGG AGCGCATGCA GCAGCACTAC 
CAGCAGGACT ACCAGCACCG CGACATGGAC TACCAGCACA GAGAGCTCGA CTACCAGAGA 
GGAGGTCCAC CAGGTGTCGG AGGTCCCGAT CACTGGACTC CACAGCCTCA GGTGTCCTCC 
TCGCTGGAGT CGTCCACGTC CAGCCAGGAG CACCTCAACT TCTCCGCCTC CTCCTCGTCC 
TCCAACAAAA CCCAGAACCA GAAGACCGGG CCGGGCCGGT GGAAGACTCC CAACGCCCCT 
CACTCCGTGC CGCCACATTC AGCCCAGCCG CCCTCCCGCT CCGACCTGCC CCCTCCTCCG 
CCTCCACCGC CAGCTACTTA CCCCGAATCC TACGACCCAT ACGACCCCCA ACCGGACCTG 
CCGCTCCCGC CGCCTCCCTC CGCCGTCACT CCTGCGGTAG CTCAGCAGGC GGCCGACCGC 
AAGAAACGTG AGGAGCAGCA GCGCTGGTAT GAGAAGGAGA AAGCTCGGCT GGAGGAGGAG 
AGGGAGAGGA AGAGGAGAGA GCAGGAGAGG AAGCTGGGTC AGATCCGGGC CAACCCCGTC 
ATGCCCGTCC AGCCTCACAA CAACGGAGGA TCCATGCCTC CGCCTCACCA CCAGCCGCCT 
CTGCCGTCTG CAGCCCCGCC TCAGCCCTAC CTTCCCCCCG CTCAGCCCCA GCCTCCACCA 
TCCAGACCGG AGAAGCTGAC CAGCCTGCCG CGGTCAGCCG TTCCTCCACC CGCCAGCGCC 
TCCAACCCTG CAGGCATGGA CACGGTGATC AGGGAGCTGT TGCCACAGCA GCAGCCGCGG 
ACCATCGAGA GGCGAGACCT GCAGTACATC ACCATCAGCA AGGAGGAGCT CACCTCCAGC 
GACAGTCTGT CTCCAGACCC CTGGAAGCGA GACGCCCGCG AGAAGGCCGA GAAGCAGCAG 
CAGCTCCACA TCGTCGACCT GCTGGACAAG GAGATCCAGG AGCTCCAGGC GAAGCCGGAG 
CGGACGGCGG AGGAGAGCGA CCGCCTCAGG AAGCTGATGC TGGAGTGGCA GTTCCAGAAA 
CGACTGCAGG AGTCCAAGCA GAGCGACGAA GACGAGGAGG AGGAGGACGA CGAGGACGTG 
GACACCATGA TGATCATGCA GAGGCTGGAG GCCGAGAAGA GAGCCAGGTT GCAGGACGAG 
GAGCGGCGGC GTAAGCAGCA GCTGGAGGAG ATTCGTAAGC GGGAGGCGGA GGAGCGAGCA 
AAGCAGGAGG AGGAGAGGAG GTGGAGGGAG GAGGAGAGAG CCAAGCGAGA GGCCGAGGAG 
AAGAGGAGAC AGGAGGAGGA GTACTACACT CGCCTGGAGG CGGAGCGGCG CCGGCAGCAC 
GACGAGGCCG AGAGGAAGCT GCTGACGCCC GACGACCCGG CCGGTCTGTA TCGGCCCCCG 
CTGCCCCGGG ATTACCAAAC CCCGCCCCCC CAACAAACCT ATAAAACCCC CCCGCCCCCC 
CCACAGAGAA ACACCTCCTA CCTCAAAACG CAGGTCACCT CCCCCGACAC GCTCTACACC 
GCCAAGTTTG TCTCCTACGC GGGCGACGAG GAGGAAGACG AGGAGGCAGG GAGTGGCGGC 
GGCGGCGGCA GCAGCAGCCA ATCAGGTCAG GTGAAGCTGT CGGCGACCCG GAAGTCGTAC 
GGCGACCTCC CGGCCTCCGC CTCGCCCAAT CACAGCAGGC CTCAGCCGCC GCCCACTGCG 
CGCAAGCCCC GCCCTGTTTC TGACGGCATC TTCCTGTCTG GCTCGTTCCA GCCGCCATTC 
AACAACAACT CCAACAGTAC AGGTCCCCCC CTTCCTGCCA AACCCAGCTC CATCCCCCCG 
GGAGGCTTTA AAGGATTTAA CTCGTACACT GGAAACTCAG CAGGAGTTGT AGGATCAGGA 
GAAGTCTACA AGGACCCGCG AGACAAATGG ACGAAGAGTC AGGAGCAGGA GAACTCGAAC 
CCCAGCGGCC CTCCGGAGAA TCTGACCTTC AAGGAGCGGC AGCGTCTCTT CTCTCAGGGG 
AAGGAGGTTT CCAACAAGGT CAAAGCTTCG CGCAAACTCA TGGAGCTGGA GAACGAGCTC 
AACACCAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027140435.1
CDS	233..6064
Translation	MSGSREEERRKLADIINHWNANRLDLFEISRPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERQLDLDEKPLVVQLNWNKDDREGRFVLKNENDILPKKSQSNGPEKEKDGVIQNFKRTLSKKEKKKEKKREKEFARIPDGDEQALSREDGENSRLAAEVYKDMPENSFTRTISNPEVVMKRRRQQKLEKRMQEFMSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTRDTADFAVVEALEKYGLEKENPREYCIARQDDKSGKEAILDDTECPLQIFRDWPADRGALVFQLKKRPPDYQGRKGRKVDDKGLRGKDGSSSLPPEKLPYLVELSPGRGNHYAYYAYRHHEDGSDSRDKPKLYRLQHSITEVGSDCTEDGAIQLLGPGILPHHCNLMHSEGMVTVTPHGPDADTFVDGQRVTETTVLRSGSTLQFGQAHVFKFVDPMFDQGGKREPAAMMRSRHKSGSVPETTFDLHGDVHSGAALPTSKSSGKLEMERGMVKPMIRGEQQDGRSQDISADRAELTLPAGIEFRDNSEDTFLSAIINYTNSSTVHFKLSPTYVLYMACRYILSPSYRPDMSPSERTHKVIAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLNRITLDAQDILAHLVQMAFKYLVHCLQADLNNYMPAFLDDPEEHNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVTDTESGLCCHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVPNIHNTCFKLNSLQLHALMNNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRSLPNGLQDFLEPLLQRGFCRLVPHPRSPGTWTVHFEGADCDSHFSAADNSEMPMRKEPEVVTVTLKKHNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRDKNGKLRPKSEGFELYNSSVQNGSPESPQAGWDSYPEPKKMSGEDRLLKNRADHRSSPNVANQGQSPAGKAVYPGGPGTKITSVSTGNLCADDEPSPPRPEAYPIPTQTYPREYFTIPASKSQDRVVGPGQGQHWQGMEDRERLPVVDNIHNSMQRVNHSQEELYPPPGGLRPEERMQQHYQQDYQHRDMDYQHRELDYQRGGPPGVGGPDHWTPQPQVSSSLESSTSSQEHLNFSASSSSSNKTQNQKTGPGRWKTPNAPHSVPPHSAQPPSRSDLPPPPPPPPATYPESYDPYDPQPDLPLPPPPSAVTPAVAQQAADRKKREEQQRWYEKEKARLEEERERKRREQERKLGQIRANPVMPVQPHNNGGSMPPPHHQPPLPSAAPPQPYLPPAQPQPPPSRPEKLTSLPRSAVPPPASASNPAGMDTVIRELLPQQQPRTIERRDLQYITISKEELTSSDSLSPDPWKRDAREKAEKQQQLHIVDLLDKEIQELQAKPERTAEESDRLRKLMLEWQFQKRLQESKQSDEDEEEEDDEDVDTMMIMQRLEAEKRARLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERAKREAEEKRRQEEEYYTRLEAERRRQHDEAERKLLTPDDPAGLYRPPLPRDYQTPPPQQTYKTPPPPPQRNTSYLKTQVTSPDTLYTAKFVSYAGDEEEDEEAGSGGGGGSSSQSGQVKLSATRKSYGDLPASASPNHSRPQPPPTARKPRPVSDGIFLSGSFQPPFNNNSNSTGPPLPAKPSSIPPGGFKGFNSYTGNSAGVVGSGEVYKDPRDKWTKSQEQENSNPSGPPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNTK

Target ORF information:

RefSeq Version	XM_027284634.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027284634.1

ORF Insert Sequence:

ATGTCGGGCA GCAGGGAGGA GGAGAGGAGG AAACTGGCCG ACATCATCAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CGGCCCACAG AGGACCTGGA GTTCCATGGC 
GTGATGCGGT TCTACTTCCA GGACCGGGTC GCCGGTAACT TTGCCACCAA ATGCATCAGA 
GTGTCGAGCA CGGCGACAAC GCAGGACGTC ATCGAGACGT TAGCAGAGAA GTTCAGACCG 
GACATGAGGA TGCTCTCCTC GCCAAAATAC TCTTTGTACG AGGTCCACGT CAGTGGCGAG 
CGTCAGCTGG ATCTGGACGA GAAGCCTCTG GTGGTTCAGC TGAACTGGAA CAAAGACGAC 
AGAGAAGGAC GCTTCGTCCT GAAGAACGAG AACGACATCC TCCCCAAGAA GAGTCAGAGT 
AACGGCCCGG AGAAGGAGAA GGACGGAGTG ATCCAGAACT TCAAGAGGAC TTTGTCCAAG 
AAGGAGAAGA AGAAGGAGAA GAAGAGGGAG AAGGAGTTCG CCCGGATCCC CGACGGAGAC 
GAGCAGGCGC TCAGCCGAGA GGACGGGGAG AACAGTCGTC TGGCGGCGGA GGTTTACAAG 
GACATGCCGG AGAACAGCTT CACCAGGACG ATCTCCAACC CGGAGGTGGT GATGAAGAGG 
AGACGCCAGC AGAAGCTGGA GAAACGCATG CAGGAGTTCA TGAGCAGCGA CGGGAGGCCC 
GACTCAGGGG GCACGTTGCG GATATACGCC GACAGCCTGA AGCCGAACAT CCCGTATAAA 
ACCATCCTGC TGTCCACGAG AGACACGGCT GACTTTGCGG TGGTGGAGGC GCTGGAGAAG 
TACGGGCTGG AGAAGGAGAA CCCCCGAGAG TACTGCATCG CCCGGCAAGA CGATAAGTCC 
GGCAAGGAGG CGATCCTGGA CGATACCGAG TGTCCACTGC AGATCTTCAG GGACTGGCCT 
GCAGACAGAG GAGCGCTAGT GTTCCAGCTG AAGAAGAGAC CCCCGGACTA CCAGGGCAGG 
AAGGGGAGGA AAGTGGACGA CAAGGGCCTG CGAGGGAAGG ACGGCAGCAG CTCCCTGCCG 
CCCGAGAAAC TGCCTTACCT GGTGGAGCTG AGCCCAGGGC GAGGGAATCA CTATGCCTAC 
TACGCCTATC GGCACCACGA AGACGGGTCT GACTCACGGG ACAAACCAAA GCTGTACCGG 
CTCCAGCACA GCATCACTGA GGTCGGCTCA GACTGCACAG AGGACGGAGC CATTCAGCTG 
CTCGGTCCGG GGATTCTGCC CCACCACTGC AACCTCATGC ACAGTGAAGG CATGGTGACG 
GTGACGCCGC ACGGCCCCGA CGCCGACACC TTCGTCGACG GGCAACGGGT CACTGAGACG 
ACGGTGCTGC GCTCGGGCTC CACCCTCCAG TTTGGCCAAG CGCACGTCTT CAAGTTTGTG 
GACCCGATGT TCGACCAGGG AGGGAAGAGA GAGCCGGCCG CCATGATGAG GAGTCGACAC 
AAGTCCGGCA GCGTACCCGA AACCACCTTC GACCTTCACG GAGACGTCCA CAGCGGAGCC 
GCGCTGCCTA CCAGCAAGAG CTCAGGAAAG CTGGAGATGG AGAGAGGAAT GGTCAAACCG 
ATGATCAGAG GAGAGCAGCA GGACGGCAGG TCTCAGGACA TTTCAGCAGA CAGAGCCGAG 
TTGACTCTGC CAGCCGGCAT CGAGTTCAGA GACAACTCTG AAGACACCTT CCTGTCGGCC 
ATCATCAACT ACACCAACAG CTCCACGGTT CACTTCAAAC TCTCGCCCAC GTACGTCCTG 
TACATGGCCT GCCGCTACAT CCTGTCGCCC TCGTACCGGC CCGACATGTC GCCGTCCGAG 
AGGACGCACA AGGTCATCGC CATCGTCAAC AAGATGGTGA GCATGATGGA GGGAGTCATC 
CAGAAGCAGA AGAACATCGC GGGAGCGCTG GCTTTCTGGA TGGCCAACGC CTCCGAGCTG 
CTCAACTTCA TCAAGCAGGA CCGAGACCTG AACCGCATCA CGCTGGACGC CCAGGACATC 
CTCGCCCATC TGGTTCAGAT GGCTTTCAAG TACCTGGTCC ACTGTCTGCA GGCTGATCTG 
AATAACTACA TGCCGGCCTT CCTGGACGAC CCCGAGGAAC ACAACCCACA GAGACCCAAG 
ATCGAGGACG TCCTGCACAC CCTCACGGGG GCGATGTCGT TACTGCGGCG GTGTCGGGTC 
AACGCCGCTC TCACCATCCA GCTCTTCTCG CAGCTCTTCC ACTTCATCAA CATGTGGCTC 
TTCAACAAGC TGGTGACGGA CACCGAGTCC GGGCTGTGCT GTCACTACTG GGGGGCCATC 
CTGCGGCAGC AGCTCAGCCA CATCGAGGCC TGGGCGGAGA AACAGGGGCT GGAGCTCGCC 
GCCGACTGCC ACCTCAGTCG AATCGTGCAG GCCACCACCC TGCTGACCAT GGACAAATAC 
TCCATGCAGG ATGTGCCGAA CATCCACAAC ACCTGCTTCA AGCTCAACTC GCTGCAGCTC 
CACGCCCTCA TGAACAACTA CCACTGCGCT CCGGACGAGC CGTACATCCC GCCGGAGCTG 
ATAGACCACG TGGTGGCAGT GGCAGAGAAC ACAGCAGATG AGCTGGCGAG GAGCGACGGC 
AGAGAGGTTC AGCTGGAGGA GGATCCGGAC CTCCAGCTGC CGTTCCTCCT TCCCGAGGAC 
GGTTACTCCT GCGACGTGGT GCGCAGCCTC CCCAACGGCC TGCAGGACTT CCTGGAGCCG 
CTGCTGCAGA GAGGTTTCTG TCGGTTGGTA CCTCACCCTC GCTCTCCCGG TACCTGGACG 
GTCCATTTTG AAGGAGCTGA CTGCGACAGC CACTTCTCTG CTGCTGACAA CTCAGAGATG 
CCAATGAGGA AAGAGCCGGA GGTTGTCACG GTAACCCTGA AGAAGCACAA CGGCATGGGC 
CTCAGCATCG TGGCTGCCAA GGGTGCTGGT CAGGAGAAGC TGGGCATCTA CATCAAGTCT 
GTCGTCAAAG GAGGAGCAGC TGATGTGGAC GGGCGTCTGG CTGCAGGTGA CCAGCTGTTG 
AGCGTGGACG GGCGGAGTCT GGTCGGCCTA TCACAAGAGA GGGCAGCGGA GCTGATGACG 
AGGACGGGCT CAGTCGTGAC TCTGGAGGTC GCCAAGCAGG GAGCCATTTA CCACGGACTG 
GCCACGCTCC TCAACCAGCC GAGCCCCATG ATGCCACGAG CGTCAGACAG AGGCCGCGAC 
AAGAACGGGA AACTCCGACC AAAGAGCGAA GGCTTCGAGT TGTACAACAG CTCGGTGCAG 
AACGGCTCTC CGGAGAGCCC GCAGGCCGGC TGGGACTCTT ACCCCGAACC AAAGAAGATG 
AGCGGCGAAG ATCGACTCCT GAAGAACCGA GCCGACCACC GCTCCAGCCC CAACGTCGCC 
AATCAGGGCC AGAGTCCTGC AGGCAAAGCA GTGTACCCCG GAGGACCTGG CACCAAGATC 
ACCTCCGTCT CCACCGGCAA CCTGTGCGCA GACGATGAGC CCTCCCCTCC ACGTCCAGAA 
GCTTACCCCA TCCCCACCCA GACCTACCCC AGGGAGTACT TCACCATCCC GGCTTCCAAG 
AGCCAGGATC GGGTCGTTGG TCCTGGGCAG GGACAGCACT GGCAGGGCAT GGAGGACAGA 
GAACGCCTCC CTGTGGTCGA CAACATCCAC AACAGCATGC AGCGGGTTAA CCACTCCCAG 
GAGGAGTTGT ACCCTCCACC CGGAGGCCTG AGGCCGGAGG AGCGCATGCA GCAGCACTAC 
CAGCAGGACT ACCAGCACCG CGACATGGAC TACCAGCACA GAGAGCTCGA CTACCAGAGA 
GGAGGTCCAC CAGGTGTCGG AGGTCCCGAT CACTGGACTC CACAGCCTCA GGTGTCCTCC 
TCGCTGGAGT CGTCCACGTC CAGCCAGGAG CACCTCAACT TCTCCGCCTC CTCCTCGTCC 
TCCAACAAAA CCCAGAACCA GAAGACCGGG CCGGGCCGGT GGAAGACTCC CAACGCCCCT 
CACTCCGTGC CGCCACATTC AGCCCAGCCG CCCTCCCGCT CCGACCTGCC CCCTCCTCCG 
CCTCCACCGC CAGCTACTTA CCCCGAATCC TACGACCCAT ACGACCCCCA ACCGGACCTG 
CCGCTCCCGC CGCCTCCCTC CGCCGTCACT CCTGCGGTAG CTCAGCAGGC GGCCGACCGC 
AAGAAACGTG AGGAGCAGCA GCGCTGGTAT GAGAAGGAGA AAGCTCGGCT GGAGGAGGAG 
AGGGAGAGGA AGAGGAGAGA GCAGGAGAGG AAGCTGGGTC AGATCCGGGC CAACCCCGTC 
ATGCCCGTCC AGCCTCACAA CAACGGAGGA TCCATGCCTC CGCCTCACCA CCAGCCGCCT 
CTGCCGTCTG CAGCCCCGCC TCAGCCCTAC CTTCCCCCCG CTCAGCCCCA GCCTCCACCA 
TCCAGACCGG AGAAGCTGAC CAGCCTGCCG CGGTCAGCCG TTCCTCCACC CGCCAGCGCC 
TCCAACCCTG CAGGCATGGA CACGGTGATC AGGGAGCTGT TGCCACAGCA GCAGCCGCGG 
ACCATCGAGA GGCGAGACCT GCAGTACATC ACCATCAGCA AGGAGGAGCT CACCTCCAGC 
GACAGTCTGT CTCCAGACCC CTGGAAGCGA GACGCCCGCG AGAAGGCCGA GAAGCAGCAG 
CAGCTCCACA TCGTCGACCT GCTGGACAAG GAGATCCAGG AGCTCCAGGC GAAGCCGGAG 
CGGACGGCGG AGGAGAGCGA CCGCCTCAGG AAGCTGATGC TGGAGTGGCA GTTCCAGAAA 
CGACTGCAGG AGTCCAAGCA GAGCGACGAA GACGAGGAGG AGGAGGACGA CGAGGACGTG 
GACACCATGA TGATCATGCA GAGGCTGGAG GCCGAGAAGA GAGCCAGGTT GCAGGACGAG 
GAGCGGCGGC GTAAGCAGCA GCTGGAGGAG ATTCGTAAGC GGGAGGCGGA GGAGCGAGCA 
AAGCAGGAGG AGGAGAGGAG GTGGAGGGAG GAGGAGAGAG CCAAGCGAGA GGCCGAGGAG 
AAGAGGAGAC AGGAGGAGGA GTACTACACT CGCCTGGAGG CGGAGCGGCG CCGGCAGCAC 
GACGAGGCCG AGAGGAAGCT GCTGACGCCC GACGACCCGG CCGGTCTGTA TCGGCCCCCG 
CTGCCCCGGG ATTACCAAAC CCCGCCCCCC CAACAAACCT ATAAAACCCC CCCGCCCCCC 
CCACAGAGAA ACACCTCCTA CCTCAAAACG CAGGTCACCT CCCCCGACAC GCTCTACACC 
GCCAAGTTTG TCTCCTACGC GGGCGACGAG GAGGAAGACG AGGAGGCAGG GAGTGGCGGC 
GGCGGCGGCA GCAGCAGCCA ATCAGGTCAG GTGAAGCTGT CGGCGACCCG GAAGTCGTAC 
GGCGACCTCC CGGCCTCCGC CTCGCCCAAT CACAGCAGGC CTCAGCCGCC GCCCACTGCG 
CGCAAGCCCC GCCCTGTTTC TGACGGCATC TTCCTGTCTG GCTCGTTCCA GCCGCCATTC 
AACAACAACT CCAACAGTAC AGGTCCCCCC CTTCCTGCCA AACCCAGCTC CATCCCCCCG 
GGAGGCTTTA AAGGATTTAA CTCGTACACT GGAAACTCAG CAGGAGTTGT AGGATCAGGA 
GAAGTCTACA AGGACCCGCG AGACAAATGG ACGAAGAGTC AGGAGCAGGA GAACTCGAAC 
CCCAGCGGCC CTCCGGAGAA TCTGACCTTC AAGGAGCGGC AGCGTCTCTT CTCTCAGGGG 
AAGGAGGTTT CCAACAAGGT CAAAGCTTCG CGCAAACTCA TGGAGCTGGA GAACGAGCTC 
AACACCAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afdn ( XM_027284636.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_027140437.1 Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X15, mRNA.

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CloneID	OLa200717
Clone ID Related Accession (Same CDS sequence)	XM_027284636.1
Accession Version	XM_027284636.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	afadin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040021.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGCA GCAGGGAGGA GGAGAGGAGG AAACTGGCCG ACATCATCAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CGGCCCACAG AGGACCTGGA GTTCCATGGC 
GTGATGCGGT TCTACTTCCA GGACCGGGTC GCCGGTAACT TTGCCACCAA ATGCATCAGA 
GTGTCGAGCA CGGCGACAAC GCAGGACGTC ATCGAGACGT TAGCAGAGAA GTTCAGACCG 
GACATGAGGA TGCTCTCCTC GCCAAAATAC TCTTTGTACG AGGTCCACGT CAGTGGCGAA 
GAGCGTCAGC TGGATCTGGA CGAGAAGCCT CTGGTGGTTC AGCTGAACTG GAACAAAGAC 
GACAGAGAAG GACGCTTCGT CCTGAAGAAC GAGAACGACA TCCTCCCCAA GAAGAGTCAG 
AGTAACGGCC CGGAGAAGGA GAAGGACGGA GTGATCCAGA ACTTCAAGAG GACTTTGTCC 
AAGAAGGAGA AGAAGAAGGA GAAGAAGAGG GAGAAGGAGT TCGCCCGGAT CCCCGACGGA 
GACGAGCAGG CGCTCAGCCG AGAGGACGGG GAGAACAGTC GTCTGGCGGC GGAGGTTTAC 
AAGGACATGC CGGAGAACAG CTTCACCAGG ACGATCTCCA ACCCGGAGGT GGTGATGAAG 
AGGAGACGCC AGCAGAAGCT GGAGAAACGC ATGCAGGAGT TCATGAGCAG CGACGGGAGG 
CCCGACTCAG GGGGCACGTT GCGGATATAC GCCGACAGCC TGAAGCCGAA CATCCCGTAT 
AAAACCATCC TGCTGTCCAC GAGAGACACG GCTGACTTTG CGGTGGTGGA GGCGCTGGAG 
AAGTACGGGC TGGAGAAGGA GAACCCCCGA GAGTACTGCA TCGCCCGGCA AGACGATAAG 
TCCGGCAAGG AGGCGATCCT GGACGATACC GAGTGTCCAC TGCAGATCTT CAGGGACTGG 
CCTGCAGACA GAGGAGCGCT AGTGTTCCAG CTGAAGAAGA GACCCCCGGA CTACCAGGGC 
AGGAAGGGGA GGAAAGTGGA CGACAAGGGC CTGCGAGGGA AGGACGGCAG CAGCTCCCTG 
CCGCCCGAGA AACTGCCTTA CCTGGTGGAG CTGAGCCCAG ACGGGTCTGA CTCACGGGAC 
AAACCAAAGC TGTACCGGCT CCAGCACAGC ATCACTGAGG TCGGCTCAGA CTGCACAGAG 
GACGGAGCCA TTCAGCTGCT CGGTCCGGGG ATTCTGCCCC ACCACTGCAA CCTCATGCAC 
AGTGAAGGCA TGGTGACGGT GACGCCGCAC GGCCCCGACG CCGACACCTT CGTCGACGGG 
CAACGGGTCA CTGAGACGAC GGTGCTGCGC TCGGGCTCCA CCCTCCAGTT TGGCCAAGCG 
CACGTCTTCA AGTTTGTGGA CCCGATGTTC GACCAGGGAG GGAAGAGAGA GCCGGCCGCC 
ATGATGAGGA GTCGACACAA GTCCGGCAGC GTACCCGAAA CCACCTTCGA CCTTCACGGA 
GACGTCCACA GCGGAGCCGC GCTGCCTACC AGCAAGAGCT CAGGAAAGCT GGAGATGGAG 
AGAGGAATGG TCAAACCGAT GATCAGAGGA GAGCAGCAGG ACGGCAGGTC TCAGGACATT 
TCAGCAGACA GAGCCGAGTT GACTCTGCCA GCCGGCATCG AGTTCAGAGA CAACTCTGAA 
GACACCTTCC TGTCGGCCAT CATCAACTAC ACCAACAGCT CCACGGTTCA CTTCAAACTC 
TCGCCCACGT ACGTCCTGTA CATGGCCTGC CGCTACATCC TGTCGCCCTC GTACCGGCCC 
GACATGTCGC CGTCCGAGAG GACGCACAAG GTCATCGCCA TCGTCAACAA GATGGTGAGC 
ATGATGGAGG GAGTCATCCA GGAGATTCCT GAAGGGGATC AGAAGCAGAA GAACATCGCG 
GGAGCGCTGG CTTTCTGGAT GGCCAACGCC TCCGAGCTGC TCAACTTCAT CAAGCAGGAC 
CGAGACCTGA ACCGCATCAC GCTGGACGCC CAGGACATCC TCGCCCATCT GGTTCAGATG 
GCTTTCAAGT ACCTGGTCCA CTGTCTGCAG GCTGATCTGA ATAACTACAT GCCGGCCTTC 
CTGGACGACC CCGAGGAACA CAACCCACAG AGACCCAAGA TCGAGGACGT CCTGCACACC 
CTCACGGGGG CGATGTCGTT ACTGCGGCGG TGTCGGGTCA ACGCCGCTCT CACCATCCAG 
CTCTTCTCGC AGCTCTTCCA CTTCATCAAC ATGTGGCTCT TCAACAAGCT GGTGACGGAC 
ACCGAGTCCG GGCTGTGCTG TCACTACTGG GGGGCCATCC TGCGGCAGCA GCTCAGCCAC 
ATCGAGGCCT GGGCGGAGAA ACAGGGGCTG GAGCTCGCCG CCGACTGCCA CCTCAGTCGA 
ATCGTGCAGG CCACCACCCT GCTGACCATG GACAAATACT CCATGCAGGA TGTGCCGAAC 
ATCCACAACA CCTGCTTCAA GCTCAACTCG CTGCAGCTCC ACGCCCTCAT GAACAACTAC 
CACTGCGCTC CGGACGAGCC GTACATCCCG CCGGAGCTGA TAGACCACGT GGTGGCAGTG 
GCAGAGAACA CAGCAGATGA GCTGGCGAGG AGCGACGGCA GAGAGGTTCA GCTGGAGGAG 
GATCCGGACC TCCAGCTGCC GTTCCTCCTT CCCGAGGACG GTTACTCCTG CGACGTGGTG 
CGCAGCCTCC CCAACGGCCT GCAGGACTTC CTGGAGCCGC TGCTGCAGAG AGGTTTCTGT 
CGGTTGGTAC CTCACCCTCG CTCTCCCGGT ACCTGGACGG TCCATTTTGA AGGAGCTGAC 
TGCGACAGCC ACTTCTCTGC TGCTGACAAC TCAGAGATGC CAATGAGGAA AGAGCCGGAG 
GTTGTCACGG TAACCCTGAA GAAGCACAAC GGCATGGGCC TCAGCATCGT GGCTGCCAAG 
GGTGCTGGTC AGGAGAAGCT GGGCATCTAC ATCAAGTCTG TCGTCAAAGG AGGAGCAGCT 
GATGTGGACG GGCGTCTGGC TGCAGGTGAC CAGCTGTTGA GCGTGGACGG GCGGAGTCTG 
GTCGGCCTAT CACAAGAGAG GGCAGCGGAG CTGATGACGA GGACGGGCTC AGTCGTGACT 
CTGGAGGTCG CCAAGCAGGG AGCCATTTAC CACGGACTGG CCACGCTCCT CAACCAGCCG 
AGCCCCATGA TGCCACGAGC GTCAGACAGA GGCCGCGACA AGAACGGGAA ACTCCGACCA 
AAGAGCGAAG GCTTCGAGTT GTACAACAGC TCGGTGCAGA ACGGCTCTCC GGAGAGCCCG 
CAGGCCGGCT GGGACTCTTA CCCCGAACCA AAGAAGATGA GCGGCGAAGA TCGACTCCTG 
AAGAACCGAG CCGACCACCG CTCCAGCCCC AACGTCGCCA ATCAGGGCCA GAGTCCTGCA 
GGCAAAGCAG TGTACCCCGG AGGACCTGGC ACCAAGATCA CCTCCGTCTC CACCGGCAAC 
CTGTGCGCAG ACGATGAGCC CTCCCCTCCA CGTCCAGAAG CTTACCCCAT CCCCACCCAG 
ACCTACCCCA GGGAGTACTT CACCATCCCG GCTTCCAAGA GCCAGGATCG GGTCGTTGGT 
CCTGGGCAGG GACAGCACTG GCAGGGCATG GAGGACAGAG AACGCCTCCC TGTGGTCGAC 
AACATCCACA ACAGCATGCA GCGGGTTAAC CACTCCCAGG AGGAGTTGTA CCCTCCACCC 
GGAGGCCTGA GGCCGGAGGA GCGCATGCAG CAGCACTACC AGCAGGACTA CCAGCACCGC 
GACATGGACT ACCAGCACAG AGAGCTCGAC TACCAGAGAG GAGGTCCACC AGGTGTCGGA 
GGTCCCGATC ACTGGACTCC ACAGCCTCAG GTGTCCTCCT CGCTGGAGTC GTCCACGTCC 
AGCCAGGAGC ACCTCAACTT CTCCGCCTCC TCCTCGTCCT CCAACAAAAC CCAGAACCAG 
AAGACCGGGC CGGGCCGGTG GAAGACTCCC AACGCCCCTC ACTCCGTGCC GCCACATTCA 
GCCCAGCCGC CCTCCCGCTC CGACCTGCCC CCTCCTCCGC CTCCACCGCC AGCTACTTAC 
CCCGAATCCT ACGACCCATA CGACCCCCAA CCGGACCTGC CGCTCCCGCC GCCTCCCTCC 
GCCGTCACTC CTGCGGTAGC TCAGCAGGCG GCCGACCGCA AGAAACGTGA GGAGCAGCAG 
CGCTGGTATG AGAAGGAGAA AGCTCGGCTG GAGGAGGAGA GGGAGAGGAA GAGGAGAGAG 
CAGGAGAGGA AGCTGGGTCA GATCCGGGCC AACCCCGTCA TGCCCGTCCA GCCTCACAAC 
AACGGAGGAT CCATGCCTCC GCCTCACCAC CAGCCGCCTC TGCCGTCTGC AGCCCCGCCT 
CAGCCCTACC TTCCCCCCGC TCAGCCCCAG CCTCCACCAT CCAGACCGGA GAAGCTGACC 
AGCCTGCCGC GGTCAGCCGT TCCTCCACCC GCCAGCGCCT CCAACCCTGC AGGCATGGAC 
ACGGTGATCA GGGAGCTGTT GCCACAGCAG CAGCCGCGGA CCATCGAGAG GCGAGACCTG 
CAGTACATCA CCATCAGCAA GGAGGAGCTC ACCTCCAGCG ACAGTCTGTC TCCAGACCCC 
TGGAAGCGAG ACGCCCGCGA GAAGGCCGAG AAGCAGCAGC AGCTCCACAT CGTCGACCTG 
CTGGACAAGG AGATCCAGGA GCTCCAGGCG AAGCCGGAGC GGACGGCGGA GGAGAGCGAC 
CGCCTCAGGA AGCTGATGCT GGAGTGGCAG TTCCAGAAAC GACTGCAGGA GTCCAAGCAG 
AGCGACGAAG ACGAGGAGGA GGAGGACGAC GAGGACGTGG ACACCATGAT GATCATGCAG 
AGGCTGGAGG CCGAGAAGAG AGCCAGGTTG CAGGACGAGG AGCGGCGGCG TAAGCAGCAG 
CTGGAGGAGA TTCGTAAGCG GGAGGCGGAG GAGCGAGCAA AGCAGGAGGA GGAGAGGAGG 
TGGAGGGAGG AGGAGAGAGC CAAGCGAGAG GCCGAGGAGA AGAGGAGACA GGAGGAGGAG 
TACTACACTC GCCTGGAGGC GGAGCGGCGC CGGCAGCACG ACGAGGCCGA GAGGAAGCTG 
CTGACGCCCG ACGACCCGGC CGGTCTGTAT CGGCCCCCGC TGCCCCGGGA TTACCAAACC 
CCGCCCCCCC AACAAACCTA TAAAACCCCC CCGCCCCCCC CACAGAGAAA CACCTCCTAC 
CTCAAAACGC AGGTCACCTC CCCCGACACG CTCTACACCG CCAAGTTTGT CTCCTACGCG 
GGCGACGAGG AGGAAGACGA GGAGGCAGGG AGTGGCGGCG GCGGCGGCAG CAGCAGCCAA 
TCAGGTCAGG TGAAGCTGTC GGCGACCCGG AAGTCGTACG GCGACCTCCC GGCCTCCGCC 
TCGCCCAATC ACAGCAGGCC TCAGCCGCCG CCCACTGCGC GCAAGCCCCG CCCTGTTTCT 
GACGGCATCT TCCTGTCTGG CTCGTTCCAG CCGCCATTCA ACAACAACTC CAACAGTACA 
GGTCCCCCCC TTCCTGCCAA ACCCAGCTCC ATCCCCCCGG GAGGCTTTAA AGGATTTAAC 
TCGTACACTG GAAACTCAGC AGGAGTTGTA GGATCAGGAG AAGTCTACAA GGACCCGCGA 
GACAAATGGA CGAAGAGTCA GGAGCAGGAG AACTCGAACC CCAGCGGCCC TCCGGAGAAT 
CTGACCTTCA AGGAGCGGCA GCGTCTCTTC TCTCAGGGGA AGGAGGTTTC CAACAAGGTC 
AAAGCTTCGC GCAAACTCAT GGAGCTGGAG AACGAGCTCA ACACCAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027140437.1
CDS	233..6043
Translation	MSGSREEERRKLADIINHWNANRLDLFEISRPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERQLDLDEKPLVVQLNWNKDDREGRFVLKNENDILPKKSQSNGPEKEKDGVIQNFKRTLSKKEKKKEKKREKEFARIPDGDEQALSREDGENSRLAAEVYKDMPENSFTRTISNPEVVMKRRRQQKLEKRMQEFMSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTRDTADFAVVEALEKYGLEKENPREYCIARQDDKSGKEAILDDTECPLQIFRDWPADRGALVFQLKKRPPDYQGRKGRKVDDKGLRGKDGSSSLPPEKLPYLVELSPDGSDSRDKPKLYRLQHSITEVGSDCTEDGAIQLLGPGILPHHCNLMHSEGMVTVTPHGPDADTFVDGQRVTETTVLRSGSTLQFGQAHVFKFVDPMFDQGGKREPAAMMRSRHKSGSVPETTFDLHGDVHSGAALPTSKSSGKLEMERGMVKPMIRGEQQDGRSQDISADRAELTLPAGIEFRDNSEDTFLSAIINYTNSSTVHFKLSPTYVLYMACRYILSPSYRPDMSPSERTHKVIAIVNKMVSMMEGVIQEIPEGDQKQKNIAGALAFWMANASELLNFIKQDRDLNRITLDAQDILAHLVQMAFKYLVHCLQADLNNYMPAFLDDPEEHNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVTDTESGLCCHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVPNIHNTCFKLNSLQLHALMNNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRSLPNGLQDFLEPLLQRGFCRLVPHPRSPGTWTVHFEGADCDSHFSAADNSEMPMRKEPEVVTVTLKKHNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRDKNGKLRPKSEGFELYNSSVQNGSPESPQAGWDSYPEPKKMSGEDRLLKNRADHRSSPNVANQGQSPAGKAVYPGGPGTKITSVSTGNLCADDEPSPPRPEAYPIPTQTYPREYFTIPASKSQDRVVGPGQGQHWQGMEDRERLPVVDNIHNSMQRVNHSQEELYPPPGGLRPEERMQQHYQQDYQHRDMDYQHRELDYQRGGPPGVGGPDHWTPQPQVSSSLESSTSSQEHLNFSASSSSSNKTQNQKTGPGRWKTPNAPHSVPPHSAQPPSRSDLPPPPPPPPATYPESYDPYDPQPDLPLPPPPSAVTPAVAQQAADRKKREEQQRWYEKEKARLEEERERKRREQERKLGQIRANPVMPVQPHNNGGSMPPPHHQPPLPSAAPPQPYLPPAQPQPPPSRPEKLTSLPRSAVPPPASASNPAGMDTVIRELLPQQQPRTIERRDLQYITISKEELTSSDSLSPDPWKRDAREKAEKQQQLHIVDLLDKEIQELQAKPERTAEESDRLRKLMLEWQFQKRLQESKQSDEDEEEEDDEDVDTMMIMQRLEAEKRARLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERAKREAEEKRRQEEEYYTRLEAERRRQHDEAERKLLTPDDPAGLYRPPLPRDYQTPPPQQTYKTPPPPPQRNTSYLKTQVTSPDTLYTAKFVSYAGDEEEDEEAGSGGGGGSSSQSGQVKLSATRKSYGDLPASASPNHSRPQPPPTARKPRPVSDGIFLSGSFQPPFNNNSNSTGPPLPAKPSSIPPGGFKGFNSYTGNSAGVVGSGEVYKDPRDKWTKSQEQENSNPSGPPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNTK

Target ORF information:

RefSeq Version	XM_027284636.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027284636.1

ORF Insert Sequence:

ATGTCGGGCA GCAGGGAGGA GGAGAGGAGG AAACTGGCCG ACATCATCAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CGGCCCACAG AGGACCTGGA GTTCCATGGC 
GTGATGCGGT TCTACTTCCA GGACCGGGTC GCCGGTAACT TTGCCACCAA ATGCATCAGA 
GTGTCGAGCA CGGCGACAAC GCAGGACGTC ATCGAGACGT TAGCAGAGAA GTTCAGACCG 
GACATGAGGA TGCTCTCCTC GCCAAAATAC TCTTTGTACG AGGTCCACGT CAGTGGCGAA 
GAGCGTCAGC TGGATCTGGA CGAGAAGCCT CTGGTGGTTC AGCTGAACTG GAACAAAGAC 
GACAGAGAAG GACGCTTCGT CCTGAAGAAC GAGAACGACA TCCTCCCCAA GAAGAGTCAG 
AGTAACGGCC CGGAGAAGGA GAAGGACGGA GTGATCCAGA ACTTCAAGAG GACTTTGTCC 
AAGAAGGAGA AGAAGAAGGA GAAGAAGAGG GAGAAGGAGT TCGCCCGGAT CCCCGACGGA 
GACGAGCAGG CGCTCAGCCG AGAGGACGGG GAGAACAGTC GTCTGGCGGC GGAGGTTTAC 
AAGGACATGC CGGAGAACAG CTTCACCAGG ACGATCTCCA ACCCGGAGGT GGTGATGAAG 
AGGAGACGCC AGCAGAAGCT GGAGAAACGC ATGCAGGAGT TCATGAGCAG CGACGGGAGG 
CCCGACTCAG GGGGCACGTT GCGGATATAC GCCGACAGCC TGAAGCCGAA CATCCCGTAT 
AAAACCATCC TGCTGTCCAC GAGAGACACG GCTGACTTTG CGGTGGTGGA GGCGCTGGAG 
AAGTACGGGC TGGAGAAGGA GAACCCCCGA GAGTACTGCA TCGCCCGGCA AGACGATAAG 
TCCGGCAAGG AGGCGATCCT GGACGATACC GAGTGTCCAC TGCAGATCTT CAGGGACTGG 
CCTGCAGACA GAGGAGCGCT AGTGTTCCAG CTGAAGAAGA GACCCCCGGA CTACCAGGGC 
AGGAAGGGGA GGAAAGTGGA CGACAAGGGC CTGCGAGGGA AGGACGGCAG CAGCTCCCTG 
CCGCCCGAGA AACTGCCTTA CCTGGTGGAG CTGAGCCCAG ACGGGTCTGA CTCACGGGAC 
AAACCAAAGC TGTACCGGCT CCAGCACAGC ATCACTGAGG TCGGCTCAGA CTGCACAGAG 
GACGGAGCCA TTCAGCTGCT CGGTCCGGGG ATTCTGCCCC ACCACTGCAA CCTCATGCAC 
AGTGAAGGCA TGGTGACGGT GACGCCGCAC GGCCCCGACG CCGACACCTT CGTCGACGGG 
CAACGGGTCA CTGAGACGAC GGTGCTGCGC TCGGGCTCCA CCCTCCAGTT TGGCCAAGCG 
CACGTCTTCA AGTTTGTGGA CCCGATGTTC GACCAGGGAG GGAAGAGAGA GCCGGCCGCC 
ATGATGAGGA GTCGACACAA GTCCGGCAGC GTACCCGAAA CCACCTTCGA CCTTCACGGA 
GACGTCCACA GCGGAGCCGC GCTGCCTACC AGCAAGAGCT CAGGAAAGCT GGAGATGGAG 
AGAGGAATGG TCAAACCGAT GATCAGAGGA GAGCAGCAGG ACGGCAGGTC TCAGGACATT 
TCAGCAGACA GAGCCGAGTT GACTCTGCCA GCCGGCATCG AGTTCAGAGA CAACTCTGAA 
GACACCTTCC TGTCGGCCAT CATCAACTAC ACCAACAGCT CCACGGTTCA CTTCAAACTC 
TCGCCCACGT ACGTCCTGTA CATGGCCTGC CGCTACATCC TGTCGCCCTC GTACCGGCCC 
GACATGTCGC CGTCCGAGAG GACGCACAAG GTCATCGCCA TCGTCAACAA GATGGTGAGC 
ATGATGGAGG GAGTCATCCA GGAGATTCCT GAAGGGGATC AGAAGCAGAA GAACATCGCG 
GGAGCGCTGG CTTTCTGGAT GGCCAACGCC TCCGAGCTGC TCAACTTCAT CAAGCAGGAC 
CGAGACCTGA ACCGCATCAC GCTGGACGCC CAGGACATCC TCGCCCATCT GGTTCAGATG 
GCTTTCAAGT ACCTGGTCCA CTGTCTGCAG GCTGATCTGA ATAACTACAT GCCGGCCTTC 
CTGGACGACC CCGAGGAACA CAACCCACAG AGACCCAAGA TCGAGGACGT CCTGCACACC 
CTCACGGGGG CGATGTCGTT ACTGCGGCGG TGTCGGGTCA ACGCCGCTCT CACCATCCAG 
CTCTTCTCGC AGCTCTTCCA CTTCATCAAC ATGTGGCTCT TCAACAAGCT GGTGACGGAC 
ACCGAGTCCG GGCTGTGCTG TCACTACTGG GGGGCCATCC TGCGGCAGCA GCTCAGCCAC 
ATCGAGGCCT GGGCGGAGAA ACAGGGGCTG GAGCTCGCCG CCGACTGCCA CCTCAGTCGA 
ATCGTGCAGG CCACCACCCT GCTGACCATG GACAAATACT CCATGCAGGA TGTGCCGAAC 
ATCCACAACA CCTGCTTCAA GCTCAACTCG CTGCAGCTCC ACGCCCTCAT GAACAACTAC 
CACTGCGCTC CGGACGAGCC GTACATCCCG CCGGAGCTGA TAGACCACGT GGTGGCAGTG 
GCAGAGAACA CAGCAGATGA GCTGGCGAGG AGCGACGGCA GAGAGGTTCA GCTGGAGGAG 
GATCCGGACC TCCAGCTGCC GTTCCTCCTT CCCGAGGACG GTTACTCCTG CGACGTGGTG 
CGCAGCCTCC CCAACGGCCT GCAGGACTTC CTGGAGCCGC TGCTGCAGAG AGGTTTCTGT 
CGGTTGGTAC CTCACCCTCG CTCTCCCGGT ACCTGGACGG TCCATTTTGA AGGAGCTGAC 
TGCGACAGCC ACTTCTCTGC TGCTGACAAC TCAGAGATGC CAATGAGGAA AGAGCCGGAG 
GTTGTCACGG TAACCCTGAA GAAGCACAAC GGCATGGGCC TCAGCATCGT GGCTGCCAAG 
GGTGCTGGTC AGGAGAAGCT GGGCATCTAC ATCAAGTCTG TCGTCAAAGG AGGAGCAGCT 
GATGTGGACG GGCGTCTGGC TGCAGGTGAC CAGCTGTTGA GCGTGGACGG GCGGAGTCTG 
GTCGGCCTAT CACAAGAGAG GGCAGCGGAG CTGATGACGA GGACGGGCTC AGTCGTGACT 
CTGGAGGTCG CCAAGCAGGG AGCCATTTAC CACGGACTGG CCACGCTCCT CAACCAGCCG 
AGCCCCATGA TGCCACGAGC GTCAGACAGA GGCCGCGACA AGAACGGGAA ACTCCGACCA 
AAGAGCGAAG GCTTCGAGTT GTACAACAGC TCGGTGCAGA ACGGCTCTCC GGAGAGCCCG 
CAGGCCGGCT GGGACTCTTA CCCCGAACCA AAGAAGATGA GCGGCGAAGA TCGACTCCTG 
AAGAACCGAG CCGACCACCG CTCCAGCCCC AACGTCGCCA ATCAGGGCCA GAGTCCTGCA 
GGCAAAGCAG TGTACCCCGG AGGACCTGGC ACCAAGATCA CCTCCGTCTC CACCGGCAAC 
CTGTGCGCAG ACGATGAGCC CTCCCCTCCA CGTCCAGAAG CTTACCCCAT CCCCACCCAG 
ACCTACCCCA GGGAGTACTT CACCATCCCG GCTTCCAAGA GCCAGGATCG GGTCGTTGGT 
CCTGGGCAGG GACAGCACTG GCAGGGCATG GAGGACAGAG AACGCCTCCC TGTGGTCGAC 
AACATCCACA ACAGCATGCA GCGGGTTAAC CACTCCCAGG AGGAGTTGTA CCCTCCACCC 
GGAGGCCTGA GGCCGGAGGA GCGCATGCAG CAGCACTACC AGCAGGACTA CCAGCACCGC 
GACATGGACT ACCAGCACAG AGAGCTCGAC TACCAGAGAG GAGGTCCACC AGGTGTCGGA 
GGTCCCGATC ACTGGACTCC ACAGCCTCAG GTGTCCTCCT CGCTGGAGTC GTCCACGTCC 
AGCCAGGAGC ACCTCAACTT CTCCGCCTCC TCCTCGTCCT CCAACAAAAC CCAGAACCAG 
AAGACCGGGC CGGGCCGGTG GAAGACTCCC AACGCCCCTC ACTCCGTGCC GCCACATTCA 
GCCCAGCCGC CCTCCCGCTC CGACCTGCCC CCTCCTCCGC CTCCACCGCC AGCTACTTAC 
CCCGAATCCT ACGACCCATA CGACCCCCAA CCGGACCTGC CGCTCCCGCC GCCTCCCTCC 
GCCGTCACTC CTGCGGTAGC TCAGCAGGCG GCCGACCGCA AGAAACGTGA GGAGCAGCAG 
CGCTGGTATG AGAAGGAGAA AGCTCGGCTG GAGGAGGAGA GGGAGAGGAA GAGGAGAGAG 
CAGGAGAGGA AGCTGGGTCA GATCCGGGCC AACCCCGTCA TGCCCGTCCA GCCTCACAAC 
AACGGAGGAT CCATGCCTCC GCCTCACCAC CAGCCGCCTC TGCCGTCTGC AGCCCCGCCT 
CAGCCCTACC TTCCCCCCGC TCAGCCCCAG CCTCCACCAT CCAGACCGGA GAAGCTGACC 
AGCCTGCCGC GGTCAGCCGT TCCTCCACCC GCCAGCGCCT CCAACCCTGC AGGCATGGAC 
ACGGTGATCA GGGAGCTGTT GCCACAGCAG CAGCCGCGGA CCATCGAGAG GCGAGACCTG 
CAGTACATCA CCATCAGCAA GGAGGAGCTC ACCTCCAGCG ACAGTCTGTC TCCAGACCCC 
TGGAAGCGAG ACGCCCGCGA GAAGGCCGAG AAGCAGCAGC AGCTCCACAT CGTCGACCTG 
CTGGACAAGG AGATCCAGGA GCTCCAGGCG AAGCCGGAGC GGACGGCGGA GGAGAGCGAC 
CGCCTCAGGA AGCTGATGCT GGAGTGGCAG TTCCAGAAAC GACTGCAGGA GTCCAAGCAG 
AGCGACGAAG ACGAGGAGGA GGAGGACGAC GAGGACGTGG ACACCATGAT GATCATGCAG 
AGGCTGGAGG CCGAGAAGAG AGCCAGGTTG CAGGACGAGG AGCGGCGGCG TAAGCAGCAG 
CTGGAGGAGA TTCGTAAGCG GGAGGCGGAG GAGCGAGCAA AGCAGGAGGA GGAGAGGAGG 
TGGAGGGAGG AGGAGAGAGC CAAGCGAGAG GCCGAGGAGA AGAGGAGACA GGAGGAGGAG 
TACTACACTC GCCTGGAGGC GGAGCGGCGC CGGCAGCACG ACGAGGCCGA GAGGAAGCTG 
CTGACGCCCG ACGACCCGGC CGGTCTGTAT CGGCCCCCGC TGCCCCGGGA TTACCAAACC 
CCGCCCCCCC AACAAACCTA TAAAACCCCC CCGCCCCCCC CACAGAGAAA CACCTCCTAC 
CTCAAAACGC AGGTCACCTC CCCCGACACG CTCTACACCG CCAAGTTTGT CTCCTACGCG 
GGCGACGAGG AGGAAGACGA GGAGGCAGGG AGTGGCGGCG GCGGCGGCAG CAGCAGCCAA 
TCAGGTCAGG TGAAGCTGTC GGCGACCCGG AAGTCGTACG GCGACCTCCC GGCCTCCGCC 
TCGCCCAATC ACAGCAGGCC TCAGCCGCCG CCCACTGCGC GCAAGCCCCG CCCTGTTTCT 
GACGGCATCT TCCTGTCTGG CTCGTTCCAG CCGCCATTCA ACAACAACTC CAACAGTACA 
GGTCCCCCCC TTCCTGCCAA ACCCAGCTCC ATCCCCCCGG GAGGCTTTAA AGGATTTAAC 
TCGTACACTG GAAACTCAGC AGGAGTTGTA GGATCAGGAG AAGTCTACAA GGACCCGCGA 
GACAAATGGA CGAAGAGTCA GGAGCAGGAG AACTCGAACC CCAGCGGCCC TCCGGAGAAT 
CTGACCTTCA AGGAGCGGCA GCGTCTCTTC TCTCAGGGGA AGGAGGTTTC CAACAAGGTC 
AAAGCTTCGC GCAAACTCAT GGAGCTGGAG AACGAGCTCA ACACCAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afdn ( XM_027284637.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_027140438.1 Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X16, mRNA.

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CloneID	OLa200718
Clone ID Related Accession (Same CDS sequence)	XM_027284637.1
Accession Version	XM_027284637.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	afadin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040021.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGCA GCAGGGAGGA GGAGAGGAGG AAACTGGCCG ACATCATCAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CGGCCCACAG AGGACCTGGA GTTCCATGGC 
GTGATGCGGT TCTACTTCCA GGACCGGGTC GCCGGTAACT TTGCCACCAA ATGCATCAGA 
GTGTCGAGCA CGGCGACAAC GCAGGACGTC ATCGAGACGT TAGCAGAGAA GTTCAGACCG 
GACATGAGGA TGCTCTCCTC GCCAAAATAC TCTTTGTACG AGGTCCACGT CAGTGGCGAG 
CGTCAGCTGG ATCTGGACGA GAAGCCTCTG GTGGTTCAGC TGAACTGGAA CAAAGACGAC 
AGAGAAGGAC GCTTCGTCCT GAAGAACGAG AACGACATCC TCCCCAAGAA GAGTCAGAGT 
AACGGCCCGG AGAAGGAGAA GGACGGAGTG ATCCAGAACT TCAAGAGGAC TTTGTCCAAG 
AAGGAGAAGA AGAAGGAGAA GAAGAGGGAG AAGGAGTTCG CCCGGATCCC CGACGGAGAC 
GAGCAGGCGC TCAGCCGAGA GGACGGGGAG AACAGTCGTC TGGCGGCGGA GGTTTACAAG 
GACATGCCGG AGAACAGCTT CACCAGGACG ATCTCCAACC CGGAGGTGGT GATGAAGAGG 
AGACGCCAGC AGAAGCTGGA GAAACGCATG CAGGAGTTCA TGAGCAGCGA CGGGAGGCCC 
GACTCAGGGG GCACGTTGCG GATATACGCC GACAGCCTGA AGCCGAACAT CCCGTATAAA 
ACCATCCTGC TGTCCACGAG AGACACGGCT GACTTTGCGG TGGTGGAGGC GCTGGAGAAG 
TACGGGCTGG AGAAGGAGAA CCCCCGAGAG TACTGCATCG CCCGGCAAGA CGATAAGTCC 
GGCAAGGAGG CGATCCTGGA CGATACCGAG TGTCCACTGC AGATCTTCAG GGACTGGCCT 
GCAGACAGAG GAGCGCTAGT GTTCCAGCTG AAGAAGAGAC CCCCGGACTA CCAGGGCAGG 
AAGGGGAGGA AAGTGGACGA CAAGGGCCTG CGAGGGAAGG ACGGCAGCAG CTCCCTGCCG 
CCCGAGAAAC TGCCTTACCT GGTGGAGCTG AGCCCAGACG GGTCTGACTC ACGGGACAAA 
CCAAAGCTGT ACCGGCTCCA GCACAGCATC ACTGAGGTCG GCTCAGACTG CACAGAGGAC 
GGAGCCATTC AGCTGCTCGG TCCGGGGATT CTGCCCCACC ACTGCAACCT CATGCACAGT 
GAAGGCATGG TGACGGTGAC GCCGCACGGC CCCGACGCCG ACACCTTCGT CGACGGGCAA 
CGGGTCACTG AGACGACGGT GCTGCGCTCG GGCTCCACCC TCCAGTTTGG CCAAGCGCAC 
GTCTTCAAGT TTGTGGACCC GATGTTCGAC CAGGGAGGGA AGAGAGAGCC GGCCGCCATG 
ATGAGGAGTC GACACAAGTC CGGCAGCGTA CCCGAAACCA CCTTCGACCT TCACGGAGAC 
GTCCACAGCG GAGCCGCGCT GCCTACCAGC AAGAGCTCAG GAAAGCTGGA GATGGAGAGA 
GGAATGGTCA AACCGATGAT CAGAGGAGAG CAGCAGGACG GCAGGTCTCA GGACATTTCA 
GCAGACAGAG CCGAGTTGAC TCTGCCAGCC GGCATCGAGT TCAGAGACAA CTCTGAAGAC 
ACCTTCCTGT CGGCCATCAT CAACTACACC AACAGCTCCA CGGTTCACTT CAAACTCTCG 
CCCACGTACG TCCTGTACAT GGCCTGCCGC TACATCCTGT CGCCCTCGTA CCGGCCCGAC 
ATGTCGCCGT CCGAGAGGAC GCACAAGGTC ATCGCCATCG TCAACAAGAT GGTGAGCATG 
ATGGAGGGAG TCATCCAGAA GCAGAAGAAC ATCGCGGGAG CGCTGGCTTT CTGGATGGCC 
AACGCCTCCG AGCTGCTCAA CTTCATCAAG CAGGACCGAG ACCTGAACCG CATCACGCTG 
GACGCCCAGG ACATCCTCGC CCATCTGGTT CAGATGGCTT TCAAGTACCT GGTCCACTGT 
CTGCAGGCTG ATCTGAATAA CTACATGCCG GCCTTCCTGG ACGACCCCGA GGAACACAAC 
CCACAGAGAC CCAAGATCGA GGACGTCCTG CACACCCTCA CGGGGGCGAT GTCGTTACTG 
CGGCGGTGTC GGGTCAACGC CGCTCTCACC ATCCAGCTCT TCTCGCAGCT CTTCCACTTC 
ATCAACATGT GGCTCTTCAA CAAGCTGGTG ACGGACACCG AGTCCGGGCT GTGCTGTCAC 
TACTGGGGGG CCATCCTGCG GCAGCAGCTC AGCCACATCG AGGCCTGGGC GGAGAAACAG 
GGGCTGGAGC TCGCCGCCGA CTGCCACCTC AGTCGAATCG TGCAGGCCAC CACCCTGCTG 
ACCATGGACA AATACTCCAT GCAGGATGTG CCGAACATCC ACAACACCTG CTTCAAGCTC 
AACTCGCTGC AGCTCCACGC CCTCATGAAC AACTACCACT GCGCTCCGGA CGAGCCGTAC 
ATCCCGCCGG AGCTGATAGA CCACGTGGTG GCAGTGGCAG AGAACACAGC AGATGAGCTG 
GCGAGGAGCG ACGGCAGAGA GGTTCAGCTG GAGGAGGATC CGGACCTCCA GCTGCCGTTC 
CTCCTTCCCG AGGACGGTTA CTCCTGCGAC GTGGTGCGCA GCCTCCCCAA CGGCCTGCAG 
GACTTCCTGG AGCCGCTGCT GCAGAGAGGT TTCTGTCGGT TGGTACCTCA CCCTCGCTCT 
CCCGGTACCT GGACGGTCCA TTTTGAAGGA GCTGACTGCG ACAGCCACTT CTCTGCTGCT 
GACAACTCAG AGATGCCAAT GAGGAAAGAG CCGGAGGTTG TCACGGTAAC CCTGAAGAAG 
CACAACGGCA TGGGCCTCAG CATCGTGGCT GCCAAGGGTG CTGGTCAGGA GAAGCTGGGC 
ATCTACATCA AGTCTGTCGT CAAAGGAGGA GCAGCTGATG TGGACGGGCG TCTGGCTGCA 
GGTGACCAGC TGTTGAGCGT GGACGGGCGG AGTCTGGTCG GCCTATCACA AGAGAGGGCA 
GCGGAGCTGA TGACGAGGAC GGGCTCAGTC GTGACTCTGG AGGTCGCCAA GCAGGGAGCC 
ATTTACCACG GACTGGCCAC GCTCCTCAAC CAGCCGAGCC CCATGATGCC ACGAGCGTCA 
GACAGAGGCC GCGACAAGAA CGGGAAACTC CGACCAAAGA GCGAAGGCTT CGAGTTGTAC 
AACAGCTCGG TGCAGAACGG CTCTCCGGAG AGCCCGCAGG CCGGCTGGGA CTCTTACCCC 
GAACCAAAGA AGATGAGCGG CGAAGATCGA CTCCTGAAGA ACCGAGCCGA CCACCGCTCC 
AGCCCCAACG TCGCCAATCA GGGCCAGAGT CCTGCAGGCA AAGCAGTGTA CCCCGGAGGA 
CCTGGCACCA AGATCACCTC CGTCTCCACC GGCAACCTGT GCGCAGACGA TGAGCCCTCC 
CCTCCACGTC CAGAAGCTTA CCCCATCCCC ACCCAGACCT ACCCCAGGGA GTACTTCACC 
ATCCCGGCTT CCAAGAGCCA GGATCGGGTC GTTGGTCCTG GGCAGGGACA GCACTGGCAG 
GGCATGGAGG ACAGAGAACG CCTCCCTGTG GTCGACAACA TCCACAACAG CATGCAGCGG 
GTTAACCACT CCCAGGAGGA GTTGTACCCT CCACCCGGAG GCCTGAGGCC GGAGGAGCGC 
ATGCAGCAGC ACTACCAGCA GGACTACCAG CACCGCGACA TGGACTACCA GCACAGAGAG 
CTCGACTACC AGAGAGGAGG TCCACCAGGT GTCGGAGGTC CCGATCACTG GACTCCACAG 
CCTCAGGTGT CCTCCTCGCT GGAGTCGTCC ACGTCCAGCC AGGAGCACCT CAACTTCTCC 
GCCTCCTCCT CGTCCTCCAA CAAAACCCAG AACCAGAAGA CCGGGCCGGG CCGGTGGAAG 
ACTCCCAACG CCCCTCACTC CGTGCCGCCA CATTCAGCCC AGCCGCCCTC CCGCTCCGAC 
CTGCCCCCTC CTCCGCCTCC ACCGCCAGCT ACTTACCCCG AATCCTACGA CCCATACGAC 
CCCCAACCGG ACCTGCCGCT CCCGCCGCCT CCCTCCGCCG TCACTCCTGC GGTAGCTCAG 
CAGGCGGCCG ACCGCAAGAA ACGTGAGGAG CAGCAGCGCT GGTATGAGAA GGAGAAAGCT 
CGGCTGGAGG AGGAGAGGGA GAGGAAGAGG AGAGAGCAGG AGAGGAAGCT GGGTCAGATC 
CGGGCCAACC CCGTCATGCC CGTCCAGCCT CACAACAACG GAGGATCCAT GCCTCCGCCT 
CACCACCAGC CGCCTCTGCC GTCTGCAGCC CCGCCTCAGC CCTACCTTCC CCCCGCTCAG 
CCCCAGCCTC CACCATCCAG ACCGGAGAAG CTGACCAGCC TGCCGCGGTC AGCCGTTCCT 
CCACCCGCCA GCGCCTCCAA CCCTGCAGGC ATGGACACGG TGATCAGGGA GCTGTTGCCA 
CAGCAGCAGC CGCGGACCAT CGAGAGGCGA GACCTGCAGT ACATCACCAT CAGCAAGGAG 
GAGCTCACCT CCAGCGACAG TCTGTCTCCA GACCCCTGGA AGCGAGACGC CCGCGAGAAG 
GCCGAGAAGC AGCAGCAGCT CCACATCGTC GACCTGCTGG ACAAGGAGAT CCAGGAGCTC 
CAGGCGAAGC CGGAGCGGAC GGCGGAGGAG AGCGACCGCC TCAGGAAGCT GATGCTGGAG 
TGGCAGTTCC AGAAACGACT GCAGGAGTCC AAGCAGAGCG ACGAAGACGA GGAGGAGGAG 
GACGACGAGG ACGTGGACAC CATGATGATC ATGCAGAGGC TGGAGGCCGA GAAGAGAGCC 
AGGTTGCAGG ACGAGGAGCG GCGGCGTAAG CAGCAGCTGG AGGAGATTCG TAAGCGGGAG 
GCGGAGGAGC GAGCAAAGCA GGAGGAGGAG AGGAGGTGGA GGGAGGAGGA GAGAGCCAAG 
CGAGAGGCCG AGGAGAAGAG GAGACAGGAG GAGGAGTACT ACACTCGCCT GGAGGCGGAG 
CGGCGCCGGC AGCACGACGA GGCCGAGAGG AAGCTGCTGA CGCCCGACGA CCCGGCCGGA 
TTTAACTCGT ACACTGGAAA CTCAGCAGGA GTTGTAGGAT CAGGAGAAGT CTACAAGGAC 
CCGCGAGACA AATGGACGAA GAGTCAGGAG CAGGAGAACT CGAACCCCAG CGGCCCTCCG 
GAGAATCTGA CCTTCAAGGA GCGGCAGCGT CTCTTCTCTC AGGGGAAGGA GGTTTCCAAC 
AAGGTCAAAG CTTCGCGCAA ACTCATGGAG CTGGAGAACG AGCTCAACAC CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027140438.1
CDS	232..5568
Translation	MSGSREEERRKLADIINHWNANRLDLFEISRPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERQLDLDEKPLVVQLNWNKDDREGRFVLKNENDILPKKSQSNGPEKEKDGVIQNFKRTLSKKEKKKEKKREKEFARIPDGDEQALSREDGENSRLAAEVYKDMPENSFTRTISNPEVVMKRRRQQKLEKRMQEFMSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTRDTADFAVVEALEKYGLEKENPREYCIARQDDKSGKEAILDDTECPLQIFRDWPADRGALVFQLKKRPPDYQGRKGRKVDDKGLRGKDGSSSLPPEKLPYLVELSPDGSDSRDKPKLYRLQHSITEVGSDCTEDGAIQLLGPGILPHHCNLMHSEGMVTVTPHGPDADTFVDGQRVTETTVLRSGSTLQFGQAHVFKFVDPMFDQGGKREPAAMMRSRHKSGSVPETTFDLHGDVHSGAALPTSKSSGKLEMERGMVKPMIRGEQQDGRSQDISADRAELTLPAGIEFRDNSEDTFLSAIINYTNSSTVHFKLSPTYVLYMACRYILSPSYRPDMSPSERTHKVIAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLNRITLDAQDILAHLVQMAFKYLVHCLQADLNNYMPAFLDDPEEHNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVTDTESGLCCHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVPNIHNTCFKLNSLQLHALMNNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRSLPNGLQDFLEPLLQRGFCRLVPHPRSPGTWTVHFEGADCDSHFSAADNSEMPMRKEPEVVTVTLKKHNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRDKNGKLRPKSEGFELYNSSVQNGSPESPQAGWDSYPEPKKMSGEDRLLKNRADHRSSPNVANQGQSPAGKAVYPGGPGTKITSVSTGNLCADDEPSPPRPEAYPIPTQTYPREYFTIPASKSQDRVVGPGQGQHWQGMEDRERLPVVDNIHNSMQRVNHSQEELYPPPGGLRPEERMQQHYQQDYQHRDMDYQHRELDYQRGGPPGVGGPDHWTPQPQVSSSLESSTSSQEHLNFSASSSSSNKTQNQKTGPGRWKTPNAPHSVPPHSAQPPSRSDLPPPPPPPPATYPESYDPYDPQPDLPLPPPPSAVTPAVAQQAADRKKREEQQRWYEKEKARLEEERERKRREQERKLGQIRANPVMPVQPHNNGGSMPPPHHQPPLPSAAPPQPYLPPAQPQPPPSRPEKLTSLPRSAVPPPASASNPAGMDTVIRELLPQQQPRTIERRDLQYITISKEELTSSDSLSPDPWKRDAREKAEKQQQLHIVDLLDKEIQELQAKPERTAEESDRLRKLMLEWQFQKRLQESKQSDEDEEEEDDEDVDTMMIMQRLEAEKRARLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERAKREAEEKRRQEEEYYTRLEAERRRQHDEAERKLLTPDDPAGFNSYTGNSAGVVGSGEVYKDPRDKWTKSQEQENSNPSGPPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNTK

Target ORF information:

RefSeq Version	XM_027284637.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027284637.1

ORF Insert Sequence:

ATGTCGGGCA GCAGGGAGGA GGAGAGGAGG AAACTGGCCG ACATCATCAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CGGCCCACAG AGGACCTGGA GTTCCATGGC 
GTGATGCGGT TCTACTTCCA GGACCGGGTC GCCGGTAACT TTGCCACCAA ATGCATCAGA 
GTGTCGAGCA CGGCGACAAC GCAGGACGTC ATCGAGACGT TAGCAGAGAA GTTCAGACCG 
GACATGAGGA TGCTCTCCTC GCCAAAATAC TCTTTGTACG AGGTCCACGT CAGTGGCGAG 
CGTCAGCTGG ATCTGGACGA GAAGCCTCTG GTGGTTCAGC TGAACTGGAA CAAAGACGAC 
AGAGAAGGAC GCTTCGTCCT GAAGAACGAG AACGACATCC TCCCCAAGAA GAGTCAGAGT 
AACGGCCCGG AGAAGGAGAA GGACGGAGTG ATCCAGAACT TCAAGAGGAC TTTGTCCAAG 
AAGGAGAAGA AGAAGGAGAA GAAGAGGGAG AAGGAGTTCG CCCGGATCCC CGACGGAGAC 
GAGCAGGCGC TCAGCCGAGA GGACGGGGAG AACAGTCGTC TGGCGGCGGA GGTTTACAAG 
GACATGCCGG AGAACAGCTT CACCAGGACG ATCTCCAACC CGGAGGTGGT GATGAAGAGG 
AGACGCCAGC AGAAGCTGGA GAAACGCATG CAGGAGTTCA TGAGCAGCGA CGGGAGGCCC 
GACTCAGGGG GCACGTTGCG GATATACGCC GACAGCCTGA AGCCGAACAT CCCGTATAAA 
ACCATCCTGC TGTCCACGAG AGACACGGCT GACTTTGCGG TGGTGGAGGC GCTGGAGAAG 
TACGGGCTGG AGAAGGAGAA CCCCCGAGAG TACTGCATCG CCCGGCAAGA CGATAAGTCC 
GGCAAGGAGG CGATCCTGGA CGATACCGAG TGTCCACTGC AGATCTTCAG GGACTGGCCT 
GCAGACAGAG GAGCGCTAGT GTTCCAGCTG AAGAAGAGAC CCCCGGACTA CCAGGGCAGG 
AAGGGGAGGA AAGTGGACGA CAAGGGCCTG CGAGGGAAGG ACGGCAGCAG CTCCCTGCCG 
CCCGAGAAAC TGCCTTACCT GGTGGAGCTG AGCCCAGACG GGTCTGACTC ACGGGACAAA 
CCAAAGCTGT ACCGGCTCCA GCACAGCATC ACTGAGGTCG GCTCAGACTG CACAGAGGAC 
GGAGCCATTC AGCTGCTCGG TCCGGGGATT CTGCCCCACC ACTGCAACCT CATGCACAGT 
GAAGGCATGG TGACGGTGAC GCCGCACGGC CCCGACGCCG ACACCTTCGT CGACGGGCAA 
CGGGTCACTG AGACGACGGT GCTGCGCTCG GGCTCCACCC TCCAGTTTGG CCAAGCGCAC 
GTCTTCAAGT TTGTGGACCC GATGTTCGAC CAGGGAGGGA AGAGAGAGCC GGCCGCCATG 
ATGAGGAGTC GACACAAGTC CGGCAGCGTA CCCGAAACCA CCTTCGACCT TCACGGAGAC 
GTCCACAGCG GAGCCGCGCT GCCTACCAGC AAGAGCTCAG GAAAGCTGGA GATGGAGAGA 
GGAATGGTCA AACCGATGAT CAGAGGAGAG CAGCAGGACG GCAGGTCTCA GGACATTTCA 
GCAGACAGAG CCGAGTTGAC TCTGCCAGCC GGCATCGAGT TCAGAGACAA CTCTGAAGAC 
ACCTTCCTGT CGGCCATCAT CAACTACACC AACAGCTCCA CGGTTCACTT CAAACTCTCG 
CCCACGTACG TCCTGTACAT GGCCTGCCGC TACATCCTGT CGCCCTCGTA CCGGCCCGAC 
ATGTCGCCGT CCGAGAGGAC GCACAAGGTC ATCGCCATCG TCAACAAGAT GGTGAGCATG 
ATGGAGGGAG TCATCCAGAA GCAGAAGAAC ATCGCGGGAG CGCTGGCTTT CTGGATGGCC 
AACGCCTCCG AGCTGCTCAA CTTCATCAAG CAGGACCGAG ACCTGAACCG CATCACGCTG 
GACGCCCAGG ACATCCTCGC CCATCTGGTT CAGATGGCTT TCAAGTACCT GGTCCACTGT 
CTGCAGGCTG ATCTGAATAA CTACATGCCG GCCTTCCTGG ACGACCCCGA GGAACACAAC 
CCACAGAGAC CCAAGATCGA GGACGTCCTG CACACCCTCA CGGGGGCGAT GTCGTTACTG 
CGGCGGTGTC GGGTCAACGC CGCTCTCACC ATCCAGCTCT TCTCGCAGCT CTTCCACTTC 
ATCAACATGT GGCTCTTCAA CAAGCTGGTG ACGGACACCG AGTCCGGGCT GTGCTGTCAC 
TACTGGGGGG CCATCCTGCG GCAGCAGCTC AGCCACATCG AGGCCTGGGC GGAGAAACAG 
GGGCTGGAGC TCGCCGCCGA CTGCCACCTC AGTCGAATCG TGCAGGCCAC CACCCTGCTG 
ACCATGGACA AATACTCCAT GCAGGATGTG CCGAACATCC ACAACACCTG CTTCAAGCTC 
AACTCGCTGC AGCTCCACGC CCTCATGAAC AACTACCACT GCGCTCCGGA CGAGCCGTAC 
ATCCCGCCGG AGCTGATAGA CCACGTGGTG GCAGTGGCAG AGAACACAGC AGATGAGCTG 
GCGAGGAGCG ACGGCAGAGA GGTTCAGCTG GAGGAGGATC CGGACCTCCA GCTGCCGTTC 
CTCCTTCCCG AGGACGGTTA CTCCTGCGAC GTGGTGCGCA GCCTCCCCAA CGGCCTGCAG 
GACTTCCTGG AGCCGCTGCT GCAGAGAGGT TTCTGTCGGT TGGTACCTCA CCCTCGCTCT 
CCCGGTACCT GGACGGTCCA TTTTGAAGGA GCTGACTGCG ACAGCCACTT CTCTGCTGCT 
GACAACTCAG AGATGCCAAT GAGGAAAGAG CCGGAGGTTG TCACGGTAAC CCTGAAGAAG 
CACAACGGCA TGGGCCTCAG CATCGTGGCT GCCAAGGGTG CTGGTCAGGA GAAGCTGGGC 
ATCTACATCA AGTCTGTCGT CAAAGGAGGA GCAGCTGATG TGGACGGGCG TCTGGCTGCA 
GGTGACCAGC TGTTGAGCGT GGACGGGCGG AGTCTGGTCG GCCTATCACA AGAGAGGGCA 
GCGGAGCTGA TGACGAGGAC GGGCTCAGTC GTGACTCTGG AGGTCGCCAA GCAGGGAGCC 
ATTTACCACG GACTGGCCAC GCTCCTCAAC CAGCCGAGCC CCATGATGCC ACGAGCGTCA 
GACAGAGGCC GCGACAAGAA CGGGAAACTC CGACCAAAGA GCGAAGGCTT CGAGTTGTAC 
AACAGCTCGG TGCAGAACGG CTCTCCGGAG AGCCCGCAGG CCGGCTGGGA CTCTTACCCC 
GAACCAAAGA AGATGAGCGG CGAAGATCGA CTCCTGAAGA ACCGAGCCGA CCACCGCTCC 
AGCCCCAACG TCGCCAATCA GGGCCAGAGT CCTGCAGGCA AAGCAGTGTA CCCCGGAGGA 
CCTGGCACCA AGATCACCTC CGTCTCCACC GGCAACCTGT GCGCAGACGA TGAGCCCTCC 
CCTCCACGTC CAGAAGCTTA CCCCATCCCC ACCCAGACCT ACCCCAGGGA GTACTTCACC 
ATCCCGGCTT CCAAGAGCCA GGATCGGGTC GTTGGTCCTG GGCAGGGACA GCACTGGCAG 
GGCATGGAGG ACAGAGAACG CCTCCCTGTG GTCGACAACA TCCACAACAG CATGCAGCGG 
GTTAACCACT CCCAGGAGGA GTTGTACCCT CCACCCGGAG GCCTGAGGCC GGAGGAGCGC 
ATGCAGCAGC ACTACCAGCA GGACTACCAG CACCGCGACA TGGACTACCA GCACAGAGAG 
CTCGACTACC AGAGAGGAGG TCCACCAGGT GTCGGAGGTC CCGATCACTG GACTCCACAG 
CCTCAGGTGT CCTCCTCGCT GGAGTCGTCC ACGTCCAGCC AGGAGCACCT CAACTTCTCC 
GCCTCCTCCT CGTCCTCCAA CAAAACCCAG AACCAGAAGA CCGGGCCGGG CCGGTGGAAG 
ACTCCCAACG CCCCTCACTC CGTGCCGCCA CATTCAGCCC AGCCGCCCTC CCGCTCCGAC 
CTGCCCCCTC CTCCGCCTCC ACCGCCAGCT ACTTACCCCG AATCCTACGA CCCATACGAC 
CCCCAACCGG ACCTGCCGCT CCCGCCGCCT CCCTCCGCCG TCACTCCTGC GGTAGCTCAG 
CAGGCGGCCG ACCGCAAGAA ACGTGAGGAG CAGCAGCGCT GGTATGAGAA GGAGAAAGCT 
CGGCTGGAGG AGGAGAGGGA GAGGAAGAGG AGAGAGCAGG AGAGGAAGCT GGGTCAGATC 
CGGGCCAACC CCGTCATGCC CGTCCAGCCT CACAACAACG GAGGATCCAT GCCTCCGCCT 
CACCACCAGC CGCCTCTGCC GTCTGCAGCC CCGCCTCAGC CCTACCTTCC CCCCGCTCAG 
CCCCAGCCTC CACCATCCAG ACCGGAGAAG CTGACCAGCC TGCCGCGGTC AGCCGTTCCT 
CCACCCGCCA GCGCCTCCAA CCCTGCAGGC ATGGACACGG TGATCAGGGA GCTGTTGCCA 
CAGCAGCAGC CGCGGACCAT CGAGAGGCGA GACCTGCAGT ACATCACCAT CAGCAAGGAG 
GAGCTCACCT CCAGCGACAG TCTGTCTCCA GACCCCTGGA AGCGAGACGC CCGCGAGAAG 
GCCGAGAAGC AGCAGCAGCT CCACATCGTC GACCTGCTGG ACAAGGAGAT CCAGGAGCTC 
CAGGCGAAGC CGGAGCGGAC GGCGGAGGAG AGCGACCGCC TCAGGAAGCT GATGCTGGAG 
TGGCAGTTCC AGAAACGACT GCAGGAGTCC AAGCAGAGCG ACGAAGACGA GGAGGAGGAG 
GACGACGAGG ACGTGGACAC CATGATGATC ATGCAGAGGC TGGAGGCCGA GAAGAGAGCC 
AGGTTGCAGG ACGAGGAGCG GCGGCGTAAG CAGCAGCTGG AGGAGATTCG TAAGCGGGAG 
GCGGAGGAGC GAGCAAAGCA GGAGGAGGAG AGGAGGTGGA GGGAGGAGGA GAGAGCCAAG 
CGAGAGGCCG AGGAGAAGAG GAGACAGGAG GAGGAGTACT ACACTCGCCT GGAGGCGGAG 
CGGCGCCGGC AGCACGACGA GGCCGAGAGG AAGCTGCTGA CGCCCGACGA CCCGGCCGGA 
TTTAACTCGT ACACTGGAAA CTCAGCAGGA GTTGTAGGAT CAGGAGAAGT CTACAAGGAC 
CCGCGAGACA AATGGACGAA GAGTCAGGAG CAGGAGAACT CGAACCCCAG CGGCCCTCCG 
GAGAATCTGA CCTTCAAGGA GCGGCAGCGT CTCTTCTCTC AGGGGAAGGA GGTTTCCAAC 
AAGGTCAAAG CTTCGCGCAA ACTCATGGAG CTGGAGAACG AGCTCAACAC CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afdn ( XM_027284638.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_027140439.1 Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X17, mRNA.

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CloneID	OLa200719
Clone ID Related Accession (Same CDS sequence)	XM_027284638.1
Accession Version	XM_027284638.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	afadin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040021.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGCA GCAGGGAGGA GGAGAGGAGG AAACTGGCCG ACATCATCAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CGGCCCACAG AGGACCTGGA GTTCCATGGC 
GTGATGCGGT TCTACTTCCA GGACCGGGTC GCCGGTAACT TTGCCACCAA ATGCATCAGA 
GTGTCGAGCA CGGCGACAAC GCAGGACGTC ATCGAGACGT TAGCAGAGAA GTTCAGACCG 
GACATGAGGA TGCTCTCCTC GCCAAAATAC TCTTTGTACG AGGTCCACGT CAGTGGCGAG 
CGTCAGCTGG ATCTGGACGA GAAGCCTCTG GTGGTTCAGC TGAACTGGAA CAAAGACGAC 
AGAGAAGGAC GCTTCGTCCT GAAGAACGAG AACGACATCC TCCCCAAGAA GAGTCAGAGT 
AACGGCCCGG AGAAGGAGAA GGACGGAGTG ATCCAGAACT TCAAGAGGAC TTTGTCCAAG 
AAGGAGAAGA AGAAGGAGAA GAAGAGGGAG AAGGAGTTCG CCCGGATCCC CGACGGAGAC 
GAGCAGGCGC TCAGCCGAGA GGACGGGGAG AACAGTCGTC TGGCGGCGGA GGTTTACAAG 
GACATGCCGG AGAACAGCTT CACCAGGACG ATCTCCAACC CGGAGGTGGT GATGAAGAGG 
AGACGCCAGC AGAAGCTGGA GAAACGCATG CAGGAGTTCA TGAGCAGCGA CGGGAGGCCC 
GACTCAGGGG GCACGTTGCG GATATACGCC GACAGCCTGA AGCCGAACAT CCCGTATAAA 
ACCATCCTGC TGTCCACGAG AGACACGGCT GACTTTGCGG TGGTGGAGGC GCTGGAGAAG 
TACGGGCTGG AGAAGGAGAA CCCCCGAGAG TACTGCATCG CCCGGCAAGA CGATAAGTCC 
GGCAAGGAGG CGATCCTGGA CGATACCGAG TGTCCACTGC AGATCTTCAG GGACTGGCCT 
GCAGACAGAG GAGCGCTAGT GTTCCAGCTG AAGAAGAGAC CCCCGGACTA CCAGGGCAGG 
AAGGGGAGGA AAGTGGACGA CAAGGGCCTG CGAGGGAAGG ACGGCAGCAG CTCCCTGCCG 
CCCGAGAAAC TGCCTTACCT GGTGGAGCTG AGCCCAGGGC GAGGGAATCA CTATGCCTAC 
TACGCCTATC GGCACCACGA AGACGGGTCT GACTCACGGG ACAAACCAAA GCTGTACCGG 
CTCCAGCACA GCATCACTGA GGTCGGCTCA GACTGCACAG AGGACGGAGC CATTCAGCTG 
CTCGGTCCGG GGATTCTGCC CCACCACTGC AACCTCATGC ACAGTGAAGG CATGGTGACG 
GTGACGCCGC ACGGCCCCGA CGCCGACACC TTCGTCGACG GGCAACGGGT CACTGAGACG 
ACGGTGCTGC GCTCGGGCTC CACCCTCCAG TTTGGCCAAG CGCACGTCTT CAAGTTTGTG 
GACCCGATGT TCGACCAGGG AGGGAAGAGA GAGCCGGCCG CCATGATGAG GAGTCGACAC 
AAGTCCGGCA GCGTACCCGA AACCACCTTC GACCTTCACG GAGACGTCCA CAGCGGAGCC 
GCGCTGCCTA CCAGCAAGAG CTCAGGAAAG CTGGAGATGG AGAGAGGAAT GGTCAAACCG 
ATGATCAGAG GAGAGCAGCA GGACGGCAGG TCTCAGGACA TTTCAGCAGA CAGAGCCGAG 
TTGACTCTGC CAGCCGGCAT CGAGTTCAGA GACAACTCTG AAGACACCTT CCTGTCGGCC 
ATCATCAACT ACACCAACAG CTCCACGGTT CACTTCAAAC TCTCGCCCAC GTACGTCCTG 
TACATGGCCT GCCGCTACAT CCTGTCGCCC TCGTACCGGC CCGACATGTC GCCGTCCGAG 
AGGACGCACA AGGTCATCGC CATCGTCAAC AAGATGGTGA GCATGATGGA GGGAGTCATC 
CAGAAGCAGA AGAACATCGC GGGAGCGCTG GCTTTCTGGA TGGCCAACGC CTCCGAGCTG 
CTCAACTTCA TCAAGCAGGA CCGAGACCTG AACCGCATCA CGCTGGACGC CCAGGACATC 
CTCGCCCATC TGGTTCAGAT GGCTTTCAAG TACCTGGTCC ACTGTCTGCA GGCTGATCTG 
AATAACTACA TGCCGGCCTT CCTGGACGAC CCCGAGGAAC ACAACCCACA GAGACCCAAG 
ATCGAGGACG TCCTGCACAC CCTCACGGGG GCGATGTCGT TACTGCGGCG GTGTCGGGTC 
AACGCCGCTC TCACCATCCA GCTCTTCTCG CAGCTCTTCC ACTTCATCAA CATGTGGCTC 
TTCAACAAGC TGGTGACGGA CACCGAGTCC GGGCTGTGCT GTCACTACTG GGGGGCCATC 
CTGCGGCAGC AGCTCAGCCA CATCGAGGCC TGGGCGGAGA AACAGGGGCT GGAGCTCGCC 
GCCGACTGCC ACCTCAGTCG AATCGTGCAG GCCACCACCC TGCTGACCAT GGACAAATAC 
TCCATGCAGG ATGTGCCGAA CATCCACAAC ACCTGCTTCA AGCTCAACTC GCTGCAGCTC 
CACGCCCTCA TGAACAACTA CCACTGCGCT CCGGACGAGC CGTACATCCC GCCGGAGCTG 
ATAGACCACG TGGTGGCAGT GGCAGAGAAC ACAGCAGATG AGCTGGCGAG GAGCGACGGC 
AGAGAGGTTC AGCTGGAGGA GGATCCGGAC CTCCAGCTGC CGTTCCTCCT TCCCGAGGAC 
GGTTACTCCT GCGACGTGGT GCGCAGCCTC CCCAACGGCC TGCAGGACTT CCTGGAGCCG 
CTGCTGCAGA GAGGTTTCTG TCGGTTGGTA CCTCACCCTC GCTCTCCCGG TACCTGGACG 
GTCCATTTTG AAGGAGCTGA CTGCGACAGC CACTTCTCTG CTGCTGACAA CTCAGAGATG 
CCAATGAGGA AAGAGCCGGA GGTTGTCACG GTAACCCTGA AGAAGCACAA CGGCATGGGC 
CTCAGCATCG TGGCTGCCAA GGGTGCTGGT CAGGAGAAGC TGGGCATCTA CATCAAGTCT 
GTCGTCAAAG GAGGAGCAGC TGATGTGGAC GGGCGTCTGG CTGCAGGTGA CCAGCTGTTG 
AGCGTGGACG GGCGGAGTCT GGTCGGCCTA TCACAAGAGA GGGCAGCGGA GCTGATGACG 
AGGACGGGCT CAGTCGTGAC TCTGGAGGTC GCCAAGCAGG GAGCCATTTA CCACGGACTG 
GCCACGCTCC TCAACCAGCC GAGCCCCATG ATGCCACGAG CGTCAGACAG AGGCCGCGAC 
AAGAACGGGA AACTCCGACC AAAGAGCGAA GGCTTCGAGT TGTACAACAG CTCGGTGCAG 
AACGGCTCTC CGGAGAGCCC GCAGGCCGGC TGGGACTCTT ACCCCGAACC AAAGAAGATG 
AGCGGCGAAG ATCGACTCCT GAAGAACCGA GCCGACCACC GCTCCAGCCC CAACGTCGCC 
AATCAGGGCC AGAGTCCTGC AGGCAAAGCA GTGTACCCCG GAGGACCTGG CACCAAGATC 
ACCTCCGTCT CCACCGGCAA CCTGTGCGCA GACGATGAGC CCTCCCCTCC ACGTCCAGAA 
GCTTACCCCA TCCCCACCCA GACCTACCCC AGGGAGTACT TCACCATCCC GGCTTCCAAG 
AGCCAGGATC GGGTCGTTGG TCCTGGGCAG GGACAGCACT GGCAGGGCAT GGAGGACAGA 
GAACGCCTCC CTGTGGTCGA CAACATCCAC AACAGCATGC AGCGGGTTAA CCACTCCCAG 
GAGGAGTTGT ACCCTCCACC CGGAGGCCTG AGGCCGGAGG AGCGCATGCA GCAGCACTAC 
CAGCAGGACT ACCAGCACCG CGACATGGAC TACCAGCACA GAGAGCTCGA CTACCAGAGA 
GGAGGTCCAC CAGGTGTCGG AGGTCCCGAT CACTGGACTC CACAGCCTCA GGTGTCCTCC 
TCGCTGGAGT CGTCCACGTC CAGCCAGGAG CACCTCAACT TCTCCGCCTC CTCCTCGTCC 
TCCAACAAAA CCCAGAACCA GAAGACCGGG CCGGGCCGGT GGAAGACTCC CAACGCCCCT 
CACTCCGTGC CGCCACATTC AGCCCAGCCG CCCTCCCGCT CCGACCTGCC CCCTCCTCCG 
CCTCCACCGC CAGCTACTTA CCCCGAATCC TACGACCCAT ACGACCCCCA ACCGGACCTG 
CCGCTCCCGC CGCCTCCCTC CGCCGTCACT CCTGCGGTAG CTCAGCAGGC GGCCGACCGC 
AAGAAACGTG AGGAGCAGCA GCGCTGGTAT GAGAAGGAGA AAGCTCGGCT GGAGGAGGAG 
AGGGAGAGGA AGAGGAGAGA GCAGGAGAGG AAGCTGGGTC AGATCCGGGC CAACCCCGTC 
ATGCCCGTCC AGCCTCACAA CAACGGAGGA TCCATGCCTC CGCCTCACCA CCAGCCGCCT 
CTGCCGTCTG CAGCCCCGCC TCAGCCCTAC CTTCCCCCCG CTCAGCCCCA GCCTCCACCA 
TCCAGACCGG AGAAGCTGAC CAGCCTGCCG CGGTCAGCCG TTCCTCCACC CGCCAGCGCC 
TCCAACCCTG CAGGCATGGA CACGGTGATC AGGGAGCTGT TGCCACAGCA GCAGCCGCGG 
ACCATCGAGA GGCGAGACCT GCAGTACATC ACCATCAGCA AGGAGGAGCT CACCTCCAGC 
GACAGTCTGT CTCCAGACCC CTGGAAGCGA GACGCCCGCG AGAAGGCCGA GAAGCAGCAG 
CAGCTCCACA TCGTCGACCT GCTGGACAAG GAGATCCAGG AGCTCCAGGC GAAGCCGGAG 
CGGACGGCGG AGGAGAGCGA CCGCCTCAGG AAGCTGATGC TGGAGTGGCA GTTCCAGAAA 
CGACTGCAGG AGTCCAAGCA GAGCGACGAA GACGAGGAGG AGGAGGACGA CGAGGACGTG 
GACACCATGA TGATCATGCA GAGGCTGGAG GCCGAGAAGA GAGCCAGGCA GAATGCTGTC 
CCAGCTATCT CTGTTCTAGA CTTGTTGCAG GACGAGGAGC GGCGGCGTAA GCAGCAGCTG 
GAGGAGATTC GTAAGCGGGA GGCGGAGGAG CGAGCAAAGC AGGAGGAGGA GAGGAGGTGG 
AGGGAGGAGG AGAGAGCCAA GCGAGAGGCC GAGGAGAAGA GGAGACAGGA GGAGGAGTAC 
TACACTCGCC TGGAGGCGGA GCGGCGCCGG CAGCACGACG AGGCCGAGAG GAAGCTGCTG 
ACGCCCGACG ACCCGGCCGG TCTGTATCGG CCCCCGCTGC CCCGGGATTA CCAAACCCCG 
CCCCCCCAAC AAACCTATAA AACCCCCCCG CCCCCCCCAC AGAGAAACAC CTCCTACCTC 
AAAACGCAGG TCACCTCCCC CGACACGCTC TACACCGCCA AGTTTGTCTC CTACGCGGGC 
GACGAGGAGG AAGACGAGGA GGCAGGGAGT GGCGGCGGCG GCGGCAGCAG CAGCCAATCA 
GGTCAGGTGA AGCTGTCGGC GACCCGGAAG TCGTACGGCG ACCTCCCGGC CTCCGCCTCG 
CCCAATCACA GCAGGCCTCA GCCGCCGCCC ACTGCGCGCA AGCCCCGCCC TGTTTCTGAC 
GGCATCTTCC TGTCTGGCTC GTTCCAGCCG CCATTCAACA ACAACTCCAA CAGTACAGGT 
CCCCCCCTTC CTGCCAAACC CAGCTCCATC CCCCCGGGAG GCTTTAAAGG ATTTAACTCG 
TACACTGGAA ACTCAGCAGG AGTTGTAGGA TCAGGAGAAG TCTACAAGGA CCCGCGAGAC 
AAATGGACGA AGAGTCAGGA GCAGGAGAAC TCGAACCCCA GCGGCCCTCC GGAGAATCTG 
ACCTTCAAGG AGCGGCAGCG TCTCTTCTCT CAGGGGAAGG AGGTTTCCAA CAAGGTCAAA 
GCTTCGCGCA AACTCATGGA GCTGGAGAAC GAGCTCAACA CCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027140439.1
CDS	233..6100
Translation	MSGSREEERRKLADIINHWNANRLDLFEISRPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERQLDLDEKPLVVQLNWNKDDREGRFVLKNENDILPKKSQSNGPEKEKDGVIQNFKRTLSKKEKKKEKKREKEFARIPDGDEQALSREDGENSRLAAEVYKDMPENSFTRTISNPEVVMKRRRQQKLEKRMQEFMSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTRDTADFAVVEALEKYGLEKENPREYCIARQDDKSGKEAILDDTECPLQIFRDWPADRGALVFQLKKRPPDYQGRKGRKVDDKGLRGKDGSSSLPPEKLPYLVELSPGRGNHYAYYAYRHHEDGSDSRDKPKLYRLQHSITEVGSDCTEDGAIQLLGPGILPHHCNLMHSEGMVTVTPHGPDADTFVDGQRVTETTVLRSGSTLQFGQAHVFKFVDPMFDQGGKREPAAMMRSRHKSGSVPETTFDLHGDVHSGAALPTSKSSGKLEMERGMVKPMIRGEQQDGRSQDISADRAELTLPAGIEFRDNSEDTFLSAIINYTNSSTVHFKLSPTYVLYMACRYILSPSYRPDMSPSERTHKVIAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLNRITLDAQDILAHLVQMAFKYLVHCLQADLNNYMPAFLDDPEEHNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVTDTESGLCCHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVPNIHNTCFKLNSLQLHALMNNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRSLPNGLQDFLEPLLQRGFCRLVPHPRSPGTWTVHFEGADCDSHFSAADNSEMPMRKEPEVVTVTLKKHNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRDKNGKLRPKSEGFELYNSSVQNGSPESPQAGWDSYPEPKKMSGEDRLLKNRADHRSSPNVANQGQSPAGKAVYPGGPGTKITSVSTGNLCADDEPSPPRPEAYPIPTQTYPREYFTIPASKSQDRVVGPGQGQHWQGMEDRERLPVVDNIHNSMQRVNHSQEELYPPPGGLRPEERMQQHYQQDYQHRDMDYQHRELDYQRGGPPGVGGPDHWTPQPQVSSSLESSTSSQEHLNFSASSSSSNKTQNQKTGPGRWKTPNAPHSVPPHSAQPPSRSDLPPPPPPPPATYPESYDPYDPQPDLPLPPPPSAVTPAVAQQAADRKKREEQQRWYEKEKARLEEERERKRREQERKLGQIRANPVMPVQPHNNGGSMPPPHHQPPLPSAAPPQPYLPPAQPQPPPSRPEKLTSLPRSAVPPPASASNPAGMDTVIRELLPQQQPRTIERRDLQYITISKEELTSSDSLSPDPWKRDAREKAEKQQQLHIVDLLDKEIQELQAKPERTAEESDRLRKLMLEWQFQKRLQESKQSDEDEEEEDDEDVDTMMIMQRLEAEKRARQNAVPAISVLDLLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERAKREAEEKRRQEEEYYTRLEAERRRQHDEAERKLLTPDDPAGLYRPPLPRDYQTPPPQQTYKTPPPPPQRNTSYLKTQVTSPDTLYTAKFVSYAGDEEEDEEAGSGGGGGSSSQSGQVKLSATRKSYGDLPASASPNHSRPQPPPTARKPRPVSDGIFLSGSFQPPFNNNSNSTGPPLPAKPSSIPPGGFKGFNSYTGNSAGVVGSGEVYKDPRDKWTKSQEQENSNPSGPPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNTK

Target ORF information:

RefSeq Version	XM_027284638.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027284638.1

ORF Insert Sequence:

ATGTCGGGCA GCAGGGAGGA GGAGAGGAGG AAACTGGCCG ACATCATCAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CGGCCCACAG AGGACCTGGA GTTCCATGGC 
GTGATGCGGT TCTACTTCCA GGACCGGGTC GCCGGTAACT TTGCCACCAA ATGCATCAGA 
GTGTCGAGCA CGGCGACAAC GCAGGACGTC ATCGAGACGT TAGCAGAGAA GTTCAGACCG 
GACATGAGGA TGCTCTCCTC GCCAAAATAC TCTTTGTACG AGGTCCACGT CAGTGGCGAG 
CGTCAGCTGG ATCTGGACGA GAAGCCTCTG GTGGTTCAGC TGAACTGGAA CAAAGACGAC 
AGAGAAGGAC GCTTCGTCCT GAAGAACGAG AACGACATCC TCCCCAAGAA GAGTCAGAGT 
AACGGCCCGG AGAAGGAGAA GGACGGAGTG ATCCAGAACT TCAAGAGGAC TTTGTCCAAG 
AAGGAGAAGA AGAAGGAGAA GAAGAGGGAG AAGGAGTTCG CCCGGATCCC CGACGGAGAC 
GAGCAGGCGC TCAGCCGAGA GGACGGGGAG AACAGTCGTC TGGCGGCGGA GGTTTACAAG 
GACATGCCGG AGAACAGCTT CACCAGGACG ATCTCCAACC CGGAGGTGGT GATGAAGAGG 
AGACGCCAGC AGAAGCTGGA GAAACGCATG CAGGAGTTCA TGAGCAGCGA CGGGAGGCCC 
GACTCAGGGG GCACGTTGCG GATATACGCC GACAGCCTGA AGCCGAACAT CCCGTATAAA 
ACCATCCTGC TGTCCACGAG AGACACGGCT GACTTTGCGG TGGTGGAGGC GCTGGAGAAG 
TACGGGCTGG AGAAGGAGAA CCCCCGAGAG TACTGCATCG CCCGGCAAGA CGATAAGTCC 
GGCAAGGAGG CGATCCTGGA CGATACCGAG TGTCCACTGC AGATCTTCAG GGACTGGCCT 
GCAGACAGAG GAGCGCTAGT GTTCCAGCTG AAGAAGAGAC CCCCGGACTA CCAGGGCAGG 
AAGGGGAGGA AAGTGGACGA CAAGGGCCTG CGAGGGAAGG ACGGCAGCAG CTCCCTGCCG 
CCCGAGAAAC TGCCTTACCT GGTGGAGCTG AGCCCAGGGC GAGGGAATCA CTATGCCTAC 
TACGCCTATC GGCACCACGA AGACGGGTCT GACTCACGGG ACAAACCAAA GCTGTACCGG 
CTCCAGCACA GCATCACTGA GGTCGGCTCA GACTGCACAG AGGACGGAGC CATTCAGCTG 
CTCGGTCCGG GGATTCTGCC CCACCACTGC AACCTCATGC ACAGTGAAGG CATGGTGACG 
GTGACGCCGC ACGGCCCCGA CGCCGACACC TTCGTCGACG GGCAACGGGT CACTGAGACG 
ACGGTGCTGC GCTCGGGCTC CACCCTCCAG TTTGGCCAAG CGCACGTCTT CAAGTTTGTG 
GACCCGATGT TCGACCAGGG AGGGAAGAGA GAGCCGGCCG CCATGATGAG GAGTCGACAC 
AAGTCCGGCA GCGTACCCGA AACCACCTTC GACCTTCACG GAGACGTCCA CAGCGGAGCC 
GCGCTGCCTA CCAGCAAGAG CTCAGGAAAG CTGGAGATGG AGAGAGGAAT GGTCAAACCG 
ATGATCAGAG GAGAGCAGCA GGACGGCAGG TCTCAGGACA TTTCAGCAGA CAGAGCCGAG 
TTGACTCTGC CAGCCGGCAT CGAGTTCAGA GACAACTCTG AAGACACCTT CCTGTCGGCC 
ATCATCAACT ACACCAACAG CTCCACGGTT CACTTCAAAC TCTCGCCCAC GTACGTCCTG 
TACATGGCCT GCCGCTACAT CCTGTCGCCC TCGTACCGGC CCGACATGTC GCCGTCCGAG 
AGGACGCACA AGGTCATCGC CATCGTCAAC AAGATGGTGA GCATGATGGA GGGAGTCATC 
CAGAAGCAGA AGAACATCGC GGGAGCGCTG GCTTTCTGGA TGGCCAACGC CTCCGAGCTG 
CTCAACTTCA TCAAGCAGGA CCGAGACCTG AACCGCATCA CGCTGGACGC CCAGGACATC 
CTCGCCCATC TGGTTCAGAT GGCTTTCAAG TACCTGGTCC ACTGTCTGCA GGCTGATCTG 
AATAACTACA TGCCGGCCTT CCTGGACGAC CCCGAGGAAC ACAACCCACA GAGACCCAAG 
ATCGAGGACG TCCTGCACAC CCTCACGGGG GCGATGTCGT TACTGCGGCG GTGTCGGGTC 
AACGCCGCTC TCACCATCCA GCTCTTCTCG CAGCTCTTCC ACTTCATCAA CATGTGGCTC 
TTCAACAAGC TGGTGACGGA CACCGAGTCC GGGCTGTGCT GTCACTACTG GGGGGCCATC 
CTGCGGCAGC AGCTCAGCCA CATCGAGGCC TGGGCGGAGA AACAGGGGCT GGAGCTCGCC 
GCCGACTGCC ACCTCAGTCG AATCGTGCAG GCCACCACCC TGCTGACCAT GGACAAATAC 
TCCATGCAGG ATGTGCCGAA CATCCACAAC ACCTGCTTCA AGCTCAACTC GCTGCAGCTC 
CACGCCCTCA TGAACAACTA CCACTGCGCT CCGGACGAGC CGTACATCCC GCCGGAGCTG 
ATAGACCACG TGGTGGCAGT GGCAGAGAAC ACAGCAGATG AGCTGGCGAG GAGCGACGGC 
AGAGAGGTTC AGCTGGAGGA GGATCCGGAC CTCCAGCTGC CGTTCCTCCT TCCCGAGGAC 
GGTTACTCCT GCGACGTGGT GCGCAGCCTC CCCAACGGCC TGCAGGACTT CCTGGAGCCG 
CTGCTGCAGA GAGGTTTCTG TCGGTTGGTA CCTCACCCTC GCTCTCCCGG TACCTGGACG 
GTCCATTTTG AAGGAGCTGA CTGCGACAGC CACTTCTCTG CTGCTGACAA CTCAGAGATG 
CCAATGAGGA AAGAGCCGGA GGTTGTCACG GTAACCCTGA AGAAGCACAA CGGCATGGGC 
CTCAGCATCG TGGCTGCCAA GGGTGCTGGT CAGGAGAAGC TGGGCATCTA CATCAAGTCT 
GTCGTCAAAG GAGGAGCAGC TGATGTGGAC GGGCGTCTGG CTGCAGGTGA CCAGCTGTTG 
AGCGTGGACG GGCGGAGTCT GGTCGGCCTA TCACAAGAGA GGGCAGCGGA GCTGATGACG 
AGGACGGGCT CAGTCGTGAC TCTGGAGGTC GCCAAGCAGG GAGCCATTTA CCACGGACTG 
GCCACGCTCC TCAACCAGCC GAGCCCCATG ATGCCACGAG CGTCAGACAG AGGCCGCGAC 
AAGAACGGGA AACTCCGACC AAAGAGCGAA GGCTTCGAGT TGTACAACAG CTCGGTGCAG 
AACGGCTCTC CGGAGAGCCC GCAGGCCGGC TGGGACTCTT ACCCCGAACC AAAGAAGATG 
AGCGGCGAAG ATCGACTCCT GAAGAACCGA GCCGACCACC GCTCCAGCCC CAACGTCGCC 
AATCAGGGCC AGAGTCCTGC AGGCAAAGCA GTGTACCCCG GAGGACCTGG CACCAAGATC 
ACCTCCGTCT CCACCGGCAA CCTGTGCGCA GACGATGAGC CCTCCCCTCC ACGTCCAGAA 
GCTTACCCCA TCCCCACCCA GACCTACCCC AGGGAGTACT TCACCATCCC GGCTTCCAAG 
AGCCAGGATC GGGTCGTTGG TCCTGGGCAG GGACAGCACT GGCAGGGCAT GGAGGACAGA 
GAACGCCTCC CTGTGGTCGA CAACATCCAC AACAGCATGC AGCGGGTTAA CCACTCCCAG 
GAGGAGTTGT ACCCTCCACC CGGAGGCCTG AGGCCGGAGG AGCGCATGCA GCAGCACTAC 
CAGCAGGACT ACCAGCACCG CGACATGGAC TACCAGCACA GAGAGCTCGA CTACCAGAGA 
GGAGGTCCAC CAGGTGTCGG AGGTCCCGAT CACTGGACTC CACAGCCTCA GGTGTCCTCC 
TCGCTGGAGT CGTCCACGTC CAGCCAGGAG CACCTCAACT TCTCCGCCTC CTCCTCGTCC 
TCCAACAAAA CCCAGAACCA GAAGACCGGG CCGGGCCGGT GGAAGACTCC CAACGCCCCT 
CACTCCGTGC CGCCACATTC AGCCCAGCCG CCCTCCCGCT CCGACCTGCC CCCTCCTCCG 
CCTCCACCGC CAGCTACTTA CCCCGAATCC TACGACCCAT ACGACCCCCA ACCGGACCTG 
CCGCTCCCGC CGCCTCCCTC CGCCGTCACT CCTGCGGTAG CTCAGCAGGC GGCCGACCGC 
AAGAAACGTG AGGAGCAGCA GCGCTGGTAT GAGAAGGAGA AAGCTCGGCT GGAGGAGGAG 
AGGGAGAGGA AGAGGAGAGA GCAGGAGAGG AAGCTGGGTC AGATCCGGGC CAACCCCGTC 
ATGCCCGTCC AGCCTCACAA CAACGGAGGA TCCATGCCTC CGCCTCACCA CCAGCCGCCT 
CTGCCGTCTG CAGCCCCGCC TCAGCCCTAC CTTCCCCCCG CTCAGCCCCA GCCTCCACCA 
TCCAGACCGG AGAAGCTGAC CAGCCTGCCG CGGTCAGCCG TTCCTCCACC CGCCAGCGCC 
TCCAACCCTG CAGGCATGGA CACGGTGATC AGGGAGCTGT TGCCACAGCA GCAGCCGCGG 
ACCATCGAGA GGCGAGACCT GCAGTACATC ACCATCAGCA AGGAGGAGCT CACCTCCAGC 
GACAGTCTGT CTCCAGACCC CTGGAAGCGA GACGCCCGCG AGAAGGCCGA GAAGCAGCAG 
CAGCTCCACA TCGTCGACCT GCTGGACAAG GAGATCCAGG AGCTCCAGGC GAAGCCGGAG 
CGGACGGCGG AGGAGAGCGA CCGCCTCAGG AAGCTGATGC TGGAGTGGCA GTTCCAGAAA 
CGACTGCAGG AGTCCAAGCA GAGCGACGAA GACGAGGAGG AGGAGGACGA CGAGGACGTG 
GACACCATGA TGATCATGCA GAGGCTGGAG GCCGAGAAGA GAGCCAGGCA GAATGCTGTC 
CCAGCTATCT CTGTTCTAGA CTTGTTGCAG GACGAGGAGC GGCGGCGTAA GCAGCAGCTG 
GAGGAGATTC GTAAGCGGGA GGCGGAGGAG CGAGCAAAGC AGGAGGAGGA GAGGAGGTGG 
AGGGAGGAGG AGAGAGCCAA GCGAGAGGCC GAGGAGAAGA GGAGACAGGA GGAGGAGTAC 
TACACTCGCC TGGAGGCGGA GCGGCGCCGG CAGCACGACG AGGCCGAGAG GAAGCTGCTG 
ACGCCCGACG ACCCGGCCGG TCTGTATCGG CCCCCGCTGC CCCGGGATTA CCAAACCCCG 
CCCCCCCAAC AAACCTATAA AACCCCCCCG CCCCCCCCAC AGAGAAACAC CTCCTACCTC 
AAAACGCAGG TCACCTCCCC CGACACGCTC TACACCGCCA AGTTTGTCTC CTACGCGGGC 
GACGAGGAGG AAGACGAGGA GGCAGGGAGT GGCGGCGGCG GCGGCAGCAG CAGCCAATCA 
GGTCAGGTGA AGCTGTCGGC GACCCGGAAG TCGTACGGCG ACCTCCCGGC CTCCGCCTCG 
CCCAATCACA GCAGGCCTCA GCCGCCGCCC ACTGCGCGCA AGCCCCGCCC TGTTTCTGAC 
GGCATCTTCC TGTCTGGCTC GTTCCAGCCG CCATTCAACA ACAACTCCAA CAGTACAGGT 
CCCCCCCTTC CTGCCAAACC CAGCTCCATC CCCCCGGGAG GCTTTAAAGG ATTTAACTCG 
TACACTGGAA ACTCAGCAGG AGTTGTAGGA TCAGGAGAAG TCTACAAGGA CCCGCGAGAC 
AAATGGACGA AGAGTCAGGA GCAGGAGAAC TCGAACCCCA GCGGCCCTCC GGAGAATCTG 
ACCTTCAAGG AGCGGCAGCG TCTCTTCTCT CAGGGGAAGG AGGTTTCCAA CAAGGTCAAA 
GCTTCGCGCA AACTCATGGA GCTGGAGAAC GAGCTCAACA CCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afdn ( XM_027284639.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_027140440.1 Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X18, mRNA.

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CloneID	OLa200720
Clone ID Related Accession (Same CDS sequence)	XM_027284639.1
Accession Version	XM_027284639.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5835bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	afadin isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040021.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGCA GCAGGGAGGA GGAGAGGAGG AAACTGGCCG ACATCATCAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CGGCCCACAG AGGACCTGGA GTTCCATGGC 
GTGATGCGGT TCTACTTCCA GGACCGGGTC GCCGGTAACT TTGCCACCAA ATGCATCAGA 
GTGTCGAGCA CGGCGACAAC GCAGGACGTC ATCGAGACGT TAGCAGAGAA GTTCAGACCG 
GACATGAGGA TGCTCTCCTC GCCAAAATAC TCTTTGTACG AGGTCCACGT CAGTGGCGAA 
GAGCGTCAGC TGGATCTGGA CGAGAAGCCT CTGGTGGTTC AGCTGAACTG GAACAAAGAC 
GACAGAGAAG GACGCTTCGT CCTGAAGAAC GAGAACGACA TCCTCCCCAA GAAGAGTCAG 
AGTAACGGCC CGGAGAAGGA GAAGGACGGA GTGATCCAGA ACTTCAAGAG GACTTTGTCC 
AAGAAGGAGA AGAAGAAGGA GAAGAAGAGG GAGAAGGAGT TCGCCCGGAT CCCCGACGGA 
GACGAGCAGG CGCTCAGCCG AGAGGACGGG GAGAACAGTC GTCTGGCGGC GGAGGTTTAC 
AAGGACATGC CGGAGAACAG CTTCACCAGG ACGATCTCCA ACCCGGAGGT GGTGATGAAG 
AGGAGACGCC AGCAGAAGCT GGAGAAACGC ATGCAGGAGT TCATGAGCAG CGACGGGAGG 
CCCGACTCAG GGGGCACGTT GCGGATATAC GCCGACAGCC TGAAGCCGAA CATCCCGTAT 
AAAACCATCC TGCTGTCCAC GAGAGACACG GCTGACTTTG CGGTGGTGGA GGCGCTGGAG 
AAGTACGGGC TGGAGAAGGA GAACCCCCGA GAGTACTGCA TCGCCCGGCA AGACGATAAG 
TCCGGCAAGG AGGCGATCCT GGACGATACC GAGTGTCCAC TGCAGATCTT CAGGGACTGG 
CCTGCAGACA GAGGAGCGCT AGTGTTCCAG CTGAAGAAGA GACCCCCGGA CTACCAGGGC 
AGGAAGGGGA GGAAAGTGGA CGACAAGGGC CTGCGAGGGA AGGACGGCAG CAGCTCCCTG 
CCGCCCGAGA AACTGCCTTA CCTGGTGGAG CTGAGCCCAG GGCGAGGGAA TCACTATGCC 
TACTACGCCT ATCGGCACCA CGAAGACGGG TCTGACTCAC GGGACAAACC AAAGCTGTAC 
CGGCTCCAGC ACAGCATCAC TGAGGTCGGC TCAGACTGCA CAGAGGACGG AGCCATTCAG 
CTGCTCGGTC CGGGGATTCT GCCCCACCAC TGCAACCTCA TGCACAGTGA AGGCATGGTG 
ACGGTGACGC CGCACGGCCC CGACGCCGAC ACCTTCGTCG ACGGGCAACG GGTCACTGAG 
ACGACGGTGC TGCGCTCGGG CTCCACCCTC CAGTTTGGCC AAGCGCACGT CTTCAAGTTT 
GTGGACCCGA TGTTCGACCA GGGAGGGAAG AGAGAGCCGG CCGCCATGAT GAGGAGTCGA 
CACAAGTCCG GCAGCGTACC CGAAACCACC TTCGACCTTC ACGGAGACGT CCACAGCGGA 
GCCGCGCTGC CTACCAGCAA GAGCTCAGGA AAGCTGGAGA TGGAGAGAGG AATGGTCAAA 
CCGATGATCA GAGGAGAGCA GCAGGACGGC AGGTCTCAGG ACATTTCAGC AGACAGAGCC 
GAGTTGACTC TGCCAGCCGG CATCGAGTTC AGAGACAACT CTGAAGACAC CTTCCTGTCG 
GCCATCATCA ACTACACCAA CAGCTCCACG GTTCACTTCA AACTCTCGCC CACGTACGTC 
CTGTACATGG CCTGCCGCTA CATCCTGTCG CCCTCGTACC GGCCCGACAT GTCGCCGTCC 
GAGAGGACGC ACAAGGTCAT CGCCATCGTC AACAAGATGG TGAGCATGAT GGAGGGAGTC 
ATCCAGAAGC AGAAGAACAT CGCGGGAGCG CTGGCTTTCT GGATGGCCAA CGCCTCCGAG 
CTGCTCAACT TCATCAAGCA GGACCGAGAC CTGAACCGCA TCACGCTGGA CGCCCAGGAC 
ATCCTCGCCC ATCTGGTTCA GATGGCTTTC AAGTACCTGG TCCACTGTCT GCAGGCTGAT 
CTGAATAACT ACATGCCGGC CTTCCTGGAC GACCCCGAGG AACACAACCC ACAGAGACCC 
AAGATCGAGG ACGTCCTGCA CACCCTCACG GGGGCGATGT CGTTACTGCG GCGGTGTCGG 
GTCAACGCCG CTCTCACCAT CCAGCTCTTC TCGCAGCTCT TCCACTTCAT CAACATGTGG 
CTCTTCAACA AGCTGGTGAC GGACACCGAG TCCGGGCTGT GCTGTCACTA CTGGGGGGCC 
ATCCTGCGGC AGCAGCTCAG CCACATCGAG GCCTGGGCGG AGAAACAGGG GCTGGAGCTC 
GCCGCCGACT GCCACCTCAG TCGAATCGTG CAGGCCACCA CCCTGCTGAC CATGGACAAA 
TACTCCATGC AGGATGTGCC GAACATCCAC AACACCTGCT TCAAGCTCAA CTCGCTGCAG 
CTCCACGCCC TCATGAACAA CTACCACTGC GCTCCGGACG AGCCGTACAT CCCGCCGGAG 
CTGATAGACC ACGTGGTGGC AGTGGCAGAG AACACAGCAG ATGAGCTGGC GAGGAGCGAC 
GGCAGAGAGG TTCAGCTGGA GGAGGATCCG GACCTCCAGC TGCCGTTCCT CCTTCCCGAG 
GACGGTTACT CCTGCGACGT GGTGCGCAGC CTCCCCAACG GCCTGCAGGA CTTCCTGGAG 
CCGCTGCTGC AGAGAGGTTT CTGTCGGTTG GTACCTCACC CTCGCTCTCC CGGTACCTGG 
ACGGTCCATT TTGAAGGAGC TGACTGCGAC AGCCACTTCT CTGCTGCTGA CAACTCAGAG 
ATGCCAATGA GGAAAGAGCC GGAGGTTGTC ACGGTAACCC TGAAGAAGCA CAACGGCATG 
GGCCTCAGCA TCGTGGCTGC CAAGGGTGCT GGTCAGGAGA AGCTGGGCAT CTACATCAAG 
TCTGTCGTCA AAGGAGGAGC AGCTGATGTG GACGGGCGTC TGGCTGCAGG TGACCAGCTG 
TTGAGCGTGG ACGGGCGGAG TCTGGTCGGC CTATCACAAG AGAGGGCAGC GGAGCTGATG 
ACGAGGACGG GCTCAGTCGT GACTCTGGAG GTCGCCAAGC AGGGAGCCAT TTACCACGGA 
CTGGCCACGC TCCTCAACCA GCCGAGCCCC ATGATGCCAC GAGCGTCAGA CAGAGGCCGC 
GACAAGAACG GGAAACTCCG ACCAAAGAGC GAAGGCTTCG AGTTGTACAA CAGCTCGGTG 
CAGAACGGCT CTCCGGAGAG CCCGCAGGCC GGCTGGGACT CTTACCCCGA ACCAAAGAAG 
ATGAGCGGCG AAGATCGACT CCTGAAGAAC CGAGCCGACC ACCGCTCCAG CCCCAACGTC 
GCCAATCAGG GCCAGAGTCC TGCAGGCAAA GCAGTGTACC CCGGAGGACC TGGCACCAAG 
ATCACCTCCG TCTCCACCGG CAACCTGTGC GCAGACGATG AGCCCTCCCC TCCACGTCCA 
GAAGCTTACC CCATCCCCAC CCAGACCTAC CCCAGGGAGT ACTTCACCAT CCCGGCTTCC 
AAGAGCCAGG ATCGGGTCGT TGGTCCTGGG CAGGGACAGC ACTGGCAGGG CATGGAGGAC 
AGAGAACGCC TCCCTGTGGT CGACAACATC CACAACAGCA TGCAGCGGGT TAACCACTCC 
CAGGAGGAGT TGTACCCTCC ACCCGGAGGC CTGAGGCCGG AGGAGCGCAT GCAGCAGCAC 
TACCAGCAGG ACTACCAGCA CCGCGACATG GACTACCAGC ACAGAGAGCT CGACTACCAG 
AGAGGAGGTC CACCAGGTGT CGGAGGTCCC GATCACTGGA CTCCACAGCC TCAGGTGTCC 
TCCTCGCTGG AGTCGTCCAC GTCCAGCCAG GAGCACCTCA ACTTCTCCGC CTCCTCCTCG 
TCCTCCAACA AAACCCAGAA CCAGAAGACC GGGCCGGGCC GGTGGAAGAC TCCCAACGCC 
CCTCACTCCG TGCCGCCACA TTCAGCCCAG CCGCCCTCCC GCTCCGACCT GCCCCCTCCT 
CCGCCTCCAC CGCCAGCTAC TTACCCCGAA TCCTACGACC CATACGACCC CCAACCGGAC 
CTGCCGCTCC CGCCGCCTCC CTCCGCCGTC ACTCCTGCGG TAGCTCAGCA GGCGGCCGAC 
CGCAAGAAAC GTGAGGAGCA GCAGCGCTGG TATGAGAAGG AGAAAGCTCG GCTGGAGGAG 
GAGAGGGAGA GGAAGAGGAG AGAGCAGGAG AGGAAGCTGG GTCAGATCCG GGCCAACCCC 
GTCATGCCCG TCCAGCCTCA CAACAACGGA GGATCCATGC CTCCGCCTCA CCACCAGCCG 
CCTCTGCCGT CTGCAGCCCC GCCTCAGCCC TACCTTCCCC CCGCTCAGCC CCAGCCTCCA 
CCATCCAGAC CGGAGAAGCT GACCAGCCTG CCGCGGTCAG CCGTTCCTCC ACCCGCCAGC 
GCCTCCAACC CTGCAGGCAT GGACACGGTG ATCAGGGAGC TGTTGCCACA GCAGCAGCCG 
CGGACCATCG AGAGGCGAGA CCTGCAGTAC ATCACCATCA GCAAGGAGGA GCTCACCTCC 
AGCGACAGTC TGTCTCCAGA CCCCTGGAAG CGAGACGCCC GCGAGAAGGC CGAGAAGCAG 
CAGCAGCTCC ACATCGTCGA CCTGCTGGAC AAGGAGATCC AGGAGCTCCA GGCGAAGCCG 
GAGCGGACGG CGGAGGAGAG CGACCGCCTC AGGAAGCTGA TGCTGGAGTG GCAGTTCCAG 
AAACGACTGC AGGAGTCCAA GCAGAGCGAC GAAGACGAGG AGGAGGAGGA CGACGAGGAC 
GTGGACACCA TGATGATCAT GCAGAGGCTG GAGGCCGAGA AGAGAGCCAG GTTGCAGGAC 
GAGGAGCGGC GGCGTAAGCA GCAGCTGGAG GAGATTCGTA AGCGGGAGGC GGAGGAGCGA 
GCAAAGCAGG AGGAGGAGAG GAGGTGGAGG GAGGAGGAGA GAGCCAAGCG AGAGGCCGAG 
GAGAAGAGGA GACAGGAGGA GGAGTACTAC ACTCGCCTGG AGGCGGAGCG GCGCCGGCAG 
CACGACGAGG CCGAGAGGAA GCTGCTGACG CCCGACGACC CGGCCGGTCT GTATCGGCCC 
CCGCTGCCCC GGGATTACCA AACCCCGCCC CCCCAACAAA CCTATAAAAC CCCCCCGCCC 
CCCCCACAGA GAAACACCTC CTACCTCAAA ACGCAGGTCA CCTCCCCCGA CACGCTCTAC 
ACCGCCAAGT TTGTCTCCTA CGCGGGCGAC GAGGAGGAAG ACGAGGAGGC AGGGAGTGGC 
GGCGGCGGCG GCAGCAGCAG CCAATCAGGT CAGGTGAAGC TGTCGGCGAC CCGGAAGTCG 
TACGGCGACC TCCCGGCCTC CGCCTCGCCC AATCACAGCA GGCCTCAGCC GCCGCCCACT 
GCGCGCAAGC CCCGCCCTGT TTCTGACGGC ATCTTCCTGT CTGGCTCGTT CCAGCCGCCA 
TTCAACAACA ACTCCAACAG TACAGGTCCC CCCCTTCCTG CCAAACCCAG CTCCATCCCC 
CCGGGAGGCT TTAAAGGATT TAACTCGTAC ACTGGAAACT CAGCAGGAGT TGTAGGATCA 
GGAGAAGTCT ACAAGGACCC GCGAGACAAA TGGACGAAGA GTCAGGAGCA GGAGAACTCG 
AACCCCAGCG GCCCTCCGGA GAATCTGACC TTCAAGGAGC GGCAGCGTCT CTTCTCTCAG 
GGGAAGGAGG TTTCCAACAA GGTCAAAGCT TCGCGCAAAC TCATGGAGCT GGAGAACGAG 
CTCAACACCA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027140440.1
CDS	233..6067
Translation	MSGSREEERRKLADIINHWNANRLDLFEISRPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERQLDLDEKPLVVQLNWNKDDREGRFVLKNENDILPKKSQSNGPEKEKDGVIQNFKRTLSKKEKKKEKKREKEFARIPDGDEQALSREDGENSRLAAEVYKDMPENSFTRTISNPEVVMKRRRQQKLEKRMQEFMSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTRDTADFAVVEALEKYGLEKENPREYCIARQDDKSGKEAILDDTECPLQIFRDWPADRGALVFQLKKRPPDYQGRKGRKVDDKGLRGKDGSSSLPPEKLPYLVELSPGRGNHYAYYAYRHHEDGSDSRDKPKLYRLQHSITEVGSDCTEDGAIQLLGPGILPHHCNLMHSEGMVTVTPHGPDADTFVDGQRVTETTVLRSGSTLQFGQAHVFKFVDPMFDQGGKREPAAMMRSRHKSGSVPETTFDLHGDVHSGAALPTSKSSGKLEMERGMVKPMIRGEQQDGRSQDISADRAELTLPAGIEFRDNSEDTFLSAIINYTNSSTVHFKLSPTYVLYMACRYILSPSYRPDMSPSERTHKVIAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLNRITLDAQDILAHLVQMAFKYLVHCLQADLNNYMPAFLDDPEEHNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVTDTESGLCCHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVPNIHNTCFKLNSLQLHALMNNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRSLPNGLQDFLEPLLQRGFCRLVPHPRSPGTWTVHFEGADCDSHFSAADNSEMPMRKEPEVVTVTLKKHNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRDKNGKLRPKSEGFELYNSSVQNGSPESPQAGWDSYPEPKKMSGEDRLLKNRADHRSSPNVANQGQSPAGKAVYPGGPGTKITSVSTGNLCADDEPSPPRPEAYPIPTQTYPREYFTIPASKSQDRVVGPGQGQHWQGMEDRERLPVVDNIHNSMQRVNHSQEELYPPPGGLRPEERMQQHYQQDYQHRDMDYQHRELDYQRGGPPGVGGPDHWTPQPQVSSSLESSTSSQEHLNFSASSSSSNKTQNQKTGPGRWKTPNAPHSVPPHSAQPPSRSDLPPPPPPPPATYPESYDPYDPQPDLPLPPPPSAVTPAVAQQAADRKKREEQQRWYEKEKARLEEERERKRREQERKLGQIRANPVMPVQPHNNGGSMPPPHHQPPLPSAAPPQPYLPPAQPQPPPSRPEKLTSLPRSAVPPPASASNPAGMDTVIRELLPQQQPRTIERRDLQYITISKEELTSSDSLSPDPWKRDAREKAEKQQQLHIVDLLDKEIQELQAKPERTAEESDRLRKLMLEWQFQKRLQESKQSDEDEEEEDDEDVDTMMIMQRLEAEKRARLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERAKREAEEKRRQEEEYYTRLEAERRRQHDEAERKLLTPDDPAGLYRPPLPRDYQTPPPQQTYKTPPPPPQRNTSYLKTQVTSPDTLYTAKFVSYAGDEEEDEEAGSGGGGGSSSQSGQVKLSATRKSYGDLPASASPNHSRPQPPPTARKPRPVSDGIFLSGSFQPPFNNNSNSTGPPLPAKPSSIPPGGFKGFNSYTGNSAGVVGSGEVYKDPRDKWTKSQEQENSNPSGPPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNTK

Target ORF information:

RefSeq Version	XM_027284639.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea afadin, adherens junction formation factor (afdn), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027284639.1

ORF Insert Sequence:

ATGTCGGGCA GCAGGGAGGA GGAGAGGAGG AAACTGGCCG ACATCATCAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CGGCCCACAG AGGACCTGGA GTTCCATGGC 
GTGATGCGGT TCTACTTCCA GGACCGGGTC GCCGGTAACT TTGCCACCAA ATGCATCAGA 
GTGTCGAGCA CGGCGACAAC GCAGGACGTC ATCGAGACGT TAGCAGAGAA GTTCAGACCG 
GACATGAGGA TGCTCTCCTC GCCAAAATAC TCTTTGTACG AGGTCCACGT CAGTGGCGAA 
GAGCGTCAGC TGGATCTGGA CGAGAAGCCT CTGGTGGTTC AGCTGAACTG GAACAAAGAC 
GACAGAGAAG GACGCTTCGT CCTGAAGAAC GAGAACGACA TCCTCCCCAA GAAGAGTCAG 
AGTAACGGCC CGGAGAAGGA GAAGGACGGA GTGATCCAGA ACTTCAAGAG GACTTTGTCC 
AAGAAGGAGA AGAAGAAGGA GAAGAAGAGG GAGAAGGAGT TCGCCCGGAT CCCCGACGGA 
GACGAGCAGG CGCTCAGCCG AGAGGACGGG GAGAACAGTC GTCTGGCGGC GGAGGTTTAC 
AAGGACATGC CGGAGAACAG CTTCACCAGG ACGATCTCCA ACCCGGAGGT GGTGATGAAG 
AGGAGACGCC AGCAGAAGCT GGAGAAACGC ATGCAGGAGT TCATGAGCAG CGACGGGAGG 
CCCGACTCAG GGGGCACGTT GCGGATATAC GCCGACAGCC TGAAGCCGAA CATCCCGTAT 
AAAACCATCC TGCTGTCCAC GAGAGACACG GCTGACTTTG CGGTGGTGGA GGCGCTGGAG 
AAGTACGGGC TGGAGAAGGA GAACCCCCGA GAGTACTGCA TCGCCCGGCA AGACGATAAG 
TCCGGCAAGG AGGCGATCCT GGACGATACC GAGTGTCCAC TGCAGATCTT CAGGGACTGG 
CCTGCAGACA GAGGAGCGCT AGTGTTCCAG CTGAAGAAGA GACCCCCGGA CTACCAGGGC 
AGGAAGGGGA GGAAAGTGGA CGACAAGGGC CTGCGAGGGA AGGACGGCAG CAGCTCCCTG 
CCGCCCGAGA AACTGCCTTA CCTGGTGGAG CTGAGCCCAG GGCGAGGGAA TCACTATGCC 
TACTACGCCT ATCGGCACCA CGAAGACGGG TCTGACTCAC GGGACAAACC AAAGCTGTAC 
CGGCTCCAGC ACAGCATCAC TGAGGTCGGC TCAGACTGCA CAGAGGACGG AGCCATTCAG 
CTGCTCGGTC CGGGGATTCT GCCCCACCAC TGCAACCTCA TGCACAGTGA AGGCATGGTG 
ACGGTGACGC CGCACGGCCC CGACGCCGAC ACCTTCGTCG ACGGGCAACG GGTCACTGAG 
ACGACGGTGC TGCGCTCGGG CTCCACCCTC CAGTTTGGCC AAGCGCACGT CTTCAAGTTT 
GTGGACCCGA TGTTCGACCA GGGAGGGAAG AGAGAGCCGG CCGCCATGAT GAGGAGTCGA 
CACAAGTCCG GCAGCGTACC CGAAACCACC TTCGACCTTC ACGGAGACGT CCACAGCGGA 
GCCGCGCTGC CTACCAGCAA GAGCTCAGGA AAGCTGGAGA TGGAGAGAGG AATGGTCAAA 
CCGATGATCA GAGGAGAGCA GCAGGACGGC AGGTCTCAGG ACATTTCAGC AGACAGAGCC 
GAGTTGACTC TGCCAGCCGG CATCGAGTTC AGAGACAACT CTGAAGACAC CTTCCTGTCG 
GCCATCATCA ACTACACCAA CAGCTCCACG GTTCACTTCA AACTCTCGCC CACGTACGTC 
CTGTACATGG CCTGCCGCTA CATCCTGTCG CCCTCGTACC GGCCCGACAT GTCGCCGTCC 
GAGAGGACGC ACAAGGTCAT CGCCATCGTC AACAAGATGG TGAGCATGAT GGAGGGAGTC 
ATCCAGAAGC AGAAGAACAT CGCGGGAGCG CTGGCTTTCT GGATGGCCAA CGCCTCCGAG 
CTGCTCAACT TCATCAAGCA GGACCGAGAC CTGAACCGCA TCACGCTGGA CGCCCAGGAC 
ATCCTCGCCC ATCTGGTTCA GATGGCTTTC AAGTACCTGG TCCACTGTCT GCAGGCTGAT 
CTGAATAACT ACATGCCGGC CTTCCTGGAC GACCCCGAGG AACACAACCC ACAGAGACCC 
AAGATCGAGG ACGTCCTGCA CACCCTCACG GGGGCGATGT CGTTACTGCG GCGGTGTCGG 
GTCAACGCCG CTCTCACCAT CCAGCTCTTC TCGCAGCTCT TCCACTTCAT CAACATGTGG 
CTCTTCAACA AGCTGGTGAC GGACACCGAG TCCGGGCTGT GCTGTCACTA CTGGGGGGCC 
ATCCTGCGGC AGCAGCTCAG CCACATCGAG GCCTGGGCGG AGAAACAGGG GCTGGAGCTC 
GCCGCCGACT GCCACCTCAG TCGAATCGTG CAGGCCACCA CCCTGCTGAC CATGGACAAA 
TACTCCATGC AGGATGTGCC GAACATCCAC AACACCTGCT TCAAGCTCAA CTCGCTGCAG 
CTCCACGCCC TCATGAACAA CTACCACTGC GCTCCGGACG AGCCGTACAT CCCGCCGGAG 
CTGATAGACC ACGTGGTGGC AGTGGCAGAG AACACAGCAG ATGAGCTGGC GAGGAGCGAC 
GGCAGAGAGG TTCAGCTGGA GGAGGATCCG GACCTCCAGC TGCCGTTCCT CCTTCCCGAG 
GACGGTTACT CCTGCGACGT GGTGCGCAGC CTCCCCAACG GCCTGCAGGA CTTCCTGGAG 
CCGCTGCTGC AGAGAGGTTT CTGTCGGTTG GTACCTCACC CTCGCTCTCC CGGTACCTGG 
ACGGTCCATT TTGAAGGAGC TGACTGCGAC AGCCACTTCT CTGCTGCTGA CAACTCAGAG 
ATGCCAATGA GGAAAGAGCC GGAGGTTGTC ACGGTAACCC TGAAGAAGCA CAACGGCATG 
GGCCTCAGCA TCGTGGCTGC CAAGGGTGCT GGTCAGGAGA AGCTGGGCAT CTACATCAAG 
TCTGTCGTCA AAGGAGGAGC AGCTGATGTG GACGGGCGTC TGGCTGCAGG TGACCAGCTG 
TTGAGCGTGG ACGGGCGGAG TCTGGTCGGC CTATCACAAG AGAGGGCAGC GGAGCTGATG 
ACGAGGACGG GCTCAGTCGT GACTCTGGAG GTCGCCAAGC AGGGAGCCAT TTACCACGGA 
CTGGCCACGC TCCTCAACCA GCCGAGCCCC ATGATGCCAC GAGCGTCAGA CAGAGGCCGC 
GACAAGAACG GGAAACTCCG ACCAAAGAGC GAAGGCTTCG AGTTGTACAA CAGCTCGGTG 
CAGAACGGCT CTCCGGAGAG CCCGCAGGCC GGCTGGGACT CTTACCCCGA ACCAAAGAAG 
ATGAGCGGCG AAGATCGACT CCTGAAGAAC CGAGCCGACC ACCGCTCCAG CCCCAACGTC 
GCCAATCAGG GCCAGAGTCC TGCAGGCAAA GCAGTGTACC CCGGAGGACC TGGCACCAAG 
ATCACCTCCG TCTCCACCGG CAACCTGTGC GCAGACGATG AGCCCTCCCC TCCACGTCCA 
GAAGCTTACC CCATCCCCAC CCAGACCTAC CCCAGGGAGT ACTTCACCAT CCCGGCTTCC 
AAGAGCCAGG ATCGGGTCGT TGGTCCTGGG CAGGGACAGC ACTGGCAGGG CATGGAGGAC 
AGAGAACGCC TCCCTGTGGT CGACAACATC CACAACAGCA TGCAGCGGGT TAACCACTCC 
CAGGAGGAGT TGTACCCTCC ACCCGGAGGC CTGAGGCCGG AGGAGCGCAT GCAGCAGCAC 
TACCAGCAGG ACTACCAGCA CCGCGACATG GACTACCAGC ACAGAGAGCT CGACTACCAG 
AGAGGAGGTC CACCAGGTGT CGGAGGTCCC GATCACTGGA CTCCACAGCC TCAGGTGTCC 
TCCTCGCTGG AGTCGTCCAC GTCCAGCCAG GAGCACCTCA ACTTCTCCGC CTCCTCCTCG 
TCCTCCAACA AAACCCAGAA CCAGAAGACC GGGCCGGGCC GGTGGAAGAC TCCCAACGCC 
CCTCACTCCG TGCCGCCACA TTCAGCCCAG CCGCCCTCCC GCTCCGACCT GCCCCCTCCT 
CCGCCTCCAC CGCCAGCTAC TTACCCCGAA TCCTACGACC CATACGACCC CCAACCGGAC 
CTGCCGCTCC CGCCGCCTCC CTCCGCCGTC ACTCCTGCGG TAGCTCAGCA GGCGGCCGAC 
CGCAAGAAAC GTGAGGAGCA GCAGCGCTGG TATGAGAAGG AGAAAGCTCG GCTGGAGGAG 
GAGAGGGAGA GGAAGAGGAG AGAGCAGGAG AGGAAGCTGG GTCAGATCCG GGCCAACCCC 
GTCATGCCCG TCCAGCCTCA CAACAACGGA GGATCCATGC CTCCGCCTCA CCACCAGCCG 
CCTCTGCCGT CTGCAGCCCC GCCTCAGCCC TACCTTCCCC CCGCTCAGCC CCAGCCTCCA 
CCATCCAGAC CGGAGAAGCT GACCAGCCTG CCGCGGTCAG CCGTTCCTCC ACCCGCCAGC 
GCCTCCAACC CTGCAGGCAT GGACACGGTG ATCAGGGAGC TGTTGCCACA GCAGCAGCCG 
CGGACCATCG AGAGGCGAGA CCTGCAGTAC ATCACCATCA GCAAGGAGGA GCTCACCTCC 
AGCGACAGTC TGTCTCCAGA CCCCTGGAAG CGAGACGCCC GCGAGAAGGC CGAGAAGCAG 
CAGCAGCTCC ACATCGTCGA CCTGCTGGAC AAGGAGATCC AGGAGCTCCA GGCGAAGCCG 
GAGCGGACGG CGGAGGAGAG CGACCGCCTC AGGAAGCTGA TGCTGGAGTG GCAGTTCCAG 
AAACGACTGC AGGAGTCCAA GCAGAGCGAC GAAGACGAGG AGGAGGAGGA CGACGAGGAC 
GTGGACACCA TGATGATCAT GCAGAGGCTG GAGGCCGAGA AGAGAGCCAG GTTGCAGGAC 
GAGGAGCGGC GGCGTAAGCA GCAGCTGGAG GAGATTCGTA AGCGGGAGGC GGAGGAGCGA 
GCAAAGCAGG AGGAGGAGAG GAGGTGGAGG GAGGAGGAGA GAGCCAAGCG AGAGGCCGAG 
GAGAAGAGGA GACAGGAGGA GGAGTACTAC ACTCGCCTGG AGGCGGAGCG GCGCCGGCAG 
CACGACGAGG CCGAGAGGAA GCTGCTGACG CCCGACGACC CGGCCGGTCT GTATCGGCCC 
CCGCTGCCCC GGGATTACCA AACCCCGCCC CCCCAACAAA CCTATAAAAC CCCCCCGCCC 
CCCCCACAGA GAAACACCTC CTACCTCAAA ACGCAGGTCA CCTCCCCCGA CACGCTCTAC 
ACCGCCAAGT TTGTCTCCTA CGCGGGCGAC GAGGAGGAAG ACGAGGAGGC AGGGAGTGGC 
GGCGGCGGCG GCAGCAGCAG CCAATCAGGT CAGGTGAAGC TGTCGGCGAC CCGGAAGTCG 
TACGGCGACC TCCCGGCCTC CGCCTCGCCC AATCACAGCA GGCCTCAGCC GCCGCCCACT 
GCGCGCAAGC CCCGCCCTGT TTCTGACGGC ATCTTCCTGT CTGGCTCGTT CCAGCCGCCA 
TTCAACAACA ACTCCAACAG TACAGGTCCC CCCCTTCCTG CCAAACCCAG CTCCATCCCC 
CCGGGAGGCT TTAAAGGATT TAACTCGTAC ACTGGAAACT CAGCAGGAGT TGTAGGATCA 
GGAGAAGTCT ACAAGGACCC GCGAGACAAA TGGACGAAGA GTCAGGAGCA GGAGAACTCG 
AACCCCAGCG GCCCTCCGGA GAATCTGACC TTCAAGGAGC GGCAGCGTCT CTTCTCTCAG 
GGGAAGGAGG TTTCCAACAA GGTCAAAGCT TCGCGCAAAC TCATGGAGCT GGAGAACGAG 
CTCAACACCA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afdn ( XM_019265734.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019121279.1 Larimichthys crocea afadin, adherens junction formation factor (afdn), mRNA.

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CloneID	OLa97801
Clone ID Related Accession (Same CDS sequence)	XM_019265734.1
Accession Version	XM_019265734.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	afadin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017608756.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

ATGTCGGGCA GCAGGGAGGA GGAGAGGAGG AAACTGGCCG ACATCATCAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CGGCCCACAG AGGACCTGGA GTTCCATGGC 
GTGATGCGGT TCTACTTCCA GGACCGGGTC GCCGGTAACT TTGCCACCAA ATGCATCAGA 
GTGTCGAGCA CGGCGACAAC GCAGGACGTC ATCGAGACGT TAGCAGAGAA GTTCAGACCG 
GACATGAGGA TGCTCTCCTC GCCAAAATAC TCTTTGTACG AGGTCCACGT CAGTGGCGAG 
CGTCAGCTGG ATCTGGACGA GAAGCCTCTG GTGGTTCAGC TGAACTGGAA CAAAGACGAC 
AGAGAAGGAC GCTTCGTCCT GAAGAACGAG AACGACATCC TCCCCAAGAA GAGTCAGAGT 
AACGGCCCGG AGAAGGAGAA GGACGGAGTG ATCCAGAACT TCAAGAGGAC TTTGTCCAAG 
AAGGAGAAGA AGAAGGAGAA GAAGAGGGAG AAGGAGTTCG CCCGGATCCC CGACGGAGAC 
GAGCAGGCGC TCAGCCGAGA GGACGGGGAG AACAGTCGTC TGGCGGCGGA GGTTTACAAG 
GACATGCCGG AGAACAGCTT CACCAGGACG ATCTCCAACC CGGAGGTGGT GATGAAGAGG 
AGACGCCAGC AGAAGCTGGA GAAACGCATG CAGGAGTTCA TGAGCAGCGA CGGGAGGCCC 
GACTCAGGGG GCACGTTGCG GATATACGCC GACAGCCTGA AGCCGAACAT CCCGTATAAA 
ACCATCCTGC TGTCCACGAG AGACACGGCT GACTTTGCGG TGGTGGAGGC GCTGGAGAAG 
TACGGGCTGG AGAAGGAGAA CCCCCGAGAG TACTGCATCG CCCGGCAAGA CGATAAGTCC 
GGCAAGGAGG CGATCCTGGA CGATACCGAG TGTCCACTGC AGATCTTCAG GGACTGGCCT 
GCAGACAGAG GAGCGCTAGT GTTCCAGCTG AAGAAGAGAC CCCCGGACTA CCAGGGCAGG 
AAGGGGAGGA AAGTGGACGA CAAGGGCCTG CGAGGGAAGG ACGGCAGCAG CTCCCTGCCG 
CCCGAGAAAC TGCCTTACCT GGTGGAGCTG AGCCCAGGGC GAGGGAATCA CTATGCCTAC 
TACGCCTATC GGCACCACGA AGACGGGTCT GACTCACGGG ACAAACCAAA GCTGTACCGG 
CTCCAGCACA GCATCACTGA GGTCGGCTCA GACTGCACAG AGGACGGAGC CATTCAGCTG 
CTCGGTCCGG GGATTCTGCC CCACCACTGC AACCTCATGC ACAGTGAAGG CATGGTGACG 
GTGACGCCGC ACGGCCCCGA CGCCGACACC TTCGTCGACG GGCAACGGGT CACTGAGACG 
ACGGTGCTGC GCTCGGGCTC CACCCTCCAG TTTGGCCAAG CGCACGTCTT CAAGTTTGTG 
GACCCGATGT TCGACCAGGG AGGGAAGAGA GAGCCGGCCG CCATGATGAG GAGTCGACAC 
AAGTCCGGCA GCGTACCCGA AACCACCTTC GACCTTCACG GAGACGTCCA CAGCGGAGCC 
GCGCTGCCTA CCAGCAAGAG CTCAGGAAAG CTGGAGATGG AGAGAGGAAT GGTCAAACCG 
ATGATCAGAG GAGAGCAGCA GGACGGCAGG TCTCAGGACA TTTCAGCAGA CAGAGCCGAG 
TTGACTCTGC CAGCCGGCAT CGAGTTCAGA GACAACTGTG ACAGCGTACC CGAAACCACC 
TTCGACCTTC ACGGAGACGT CCACAGCGGA GCCGCGCTGC CTACCAGCAA GAGCTCAGGA 
AAGCTGGAGA TGGAGAGAGG AATGGTCAAA CCGATGATCA GAGGAGAGCA GCAGGACGGC 
AGGTCTCAGG ACATTTCAGC AGACAGAGCC GAGTTGACTC TGCCAGCCGG CATCGAGTTC 
AGAGACAACT CTGAAGACAC CTTCCTGTCG GCCATCATCA ACTACACCAA CAGCTCCACG 
GTTCACTTCA AACTCTCGCC CACGTACGTC CTGTACATGG CCTGCCGCTA CATCCTGTCG 
CCCTCGTACC GGCCCGACAT GTCGCCGTCC GAGAGGACGC ACAAGGTCAT CGCCATCGTC 
AACAAGATGG TGAGCATGAT GGAGGGAGTC ATCCAGAAGC AGAAGAACAT CGCGGGAGCG 
CTGGCTTTCT GGATGGCCAA CGCCTCCGAG CTGCTCAACT TCATCAAGCA GGACCGAGAC 
CTGAACCGCA TCACGCTGGA CGCCCAGGAC ATCCTCGCCC ATCTGGTTCA GATGGCTTTC 
AAGTACCTGG TCCACTGTCT GCAGGCTGAT CTGAATAACT ACATGCCGGC CTTCCTGGAC 
GACCCCGAGG AACACAACCC ACAGAGACCC AAGATCGAGG ACGTCCTGCA CACCCTCACG 
GGGGCGATGT CGTTACTGCG GCGGTGTCGG GTCAACGCCG CTCTCACCAT CCAGCTCTTC 
TCGCAGCTCT TCCACTTCAT CAACATGTGG CTCTTCAACA AGCTGGTGAC GGACACCGAG 
TCCGGGCTGT GCTGTCACTA CTGGGGGGCC ATCCTGCGGC AGCAGCTCAG CCACATCGAG 
GCCTGGGCGG AGAAACAGGG GCTGGAGCTC GCCGCCGACT GCCACCTCAG TCGAATCGTG 
CAGGCCACCA CCCTGCTGAC CATGGACAAA TACTCCATGC AGGATGTGCC GAACATCCAC 
AACACCTGCT TCAAGCTCAA CTCGCTGCAG CTCCACGCCC TCATGAACAA CTACCACTGC 
GCTCCGGACG AGCCGTACAT CCCGCCGGAG CTGATAGACC ACGTGGTGGC AGTGGCAGAG 
AACACAGCAG ATGAGCTGGC GAGGAGCGAC GGCAGAGAGG TTCAGCTGGA GGAGGATCCG 
GACCTCCAGC TGCCGTTCCT CCTTCCCGAG GACGGTTACT CCTGCGACGT GGTGCGCAGC 
CTCCCCAACG GCCTGCAGGA CTTCCTGGAG CCGCTGCTGC AGAGAGGTTT CTGTCGGTTG 
GTACCTCACC CTCGCTCTCC CGGTACCTGG ACGGTCCATT TTGAAGGAGC TGACTGCGAC 
AGCCACTTCT CTGCTGCTGA CAACTCAGAG ATGCCAATGA GGAAAGAGCC GGAGGTTGTC 
ACGGTAACCC TGAAGAAGCA CAACGGCATG GGCCTCAGCA TCGTGGCTGC CAAGGGTGCT 
GGTCAGGAGA AGCTGGGCAT CTACATCAAG TCTGTCGTCA AAGGAGGAGC AGCTGATGTG 
GACGGGCGTC TGGCTGCAGG TGACCAGCTG TTGAGCGTGG ACGGGCGGAG TCTGGTCGGC 
CTATCACAAG AGAGGGCAGC GGAGCTGATG ACGAGGACGG GCTCAGTCGT GACTCTGGAG 
GTCGCCAAGC AGGGAGCCAT TTACCACGGA CTGGCCACGC TCCTCAACCA GCCGAGCCCC 
ATGATGCCAC GAGCGTCAGA CAGAGGCCGC GACAAGAACG GGAAACTCCG ACCAAAGAGC 
GAAGGCTTCG AGTTGTACAA CAGCTCGGTG CAGAACGGCT CTCCGGAGAG CCCGCAGGCC 
GGCTGGGACT CTTACCCCGA ACCAAAGAAG ATGAGCGGCG AAGATCGACT CCTGAAGAAC 
CGAGCCGACC ACCGCTCCAG CCCCAACGTC GCCAATCAGG GCCAGAGTCC TGCAGGCAAA 
GCAGTGTACC CCGGAGGACC TGGCACCAAG ATCACCTCCG TCTCCACCGG CAACCTGTGC 
GCAGACGATG AGCCCTCCCC TCCACGTCCA GAAGCTTACC CCATCCCCAC CCAGACCTAC 
CCCAGGGAGT ACTTCACCAT CCCGGCTTCC AAGAGCCAGG ATCGGGTCGT TGGTCCTGGG 
CAGGGACAGC ACTGGCAGGG CATGGAGGAC AGAGAACGCC TCCCTGTGGT CGACAACATC 
CACAACAGCA TGCAGCGGGT TAACCACTCC CAGGAGGAGT TGTACCCTCC ACCCGGAGGC 
CTGAGGCCGG AGGAGCGCAT GCAGCAGCAC TACCAGCAGG ACTACCAGCA CCGCGACATG 
GACTACCAGC ACAGAGAGCT CGACTACCAG AGAGGAGGTC CACCAGGTGT CGGAGGTCCC 
GATCACTGGA CTCCACAGCC TCAGGTGTCC TCCTCGCTGG AGTCGTCCAC GTCCAGCCAG 
GAGCACCTCA ACTTCTCCGC CTCCTCCTCG TCCTCCAACA AAACCCAGAA CCAGAAGACC 
GGGCCGGGCC GGTGGAAGAC TCCCAACGCC CCTCACTCCG TGCCGCCACA TTCAGCCCAG 
CCGCCCTCCC GCTCCGACCT GCCCCCTCCT CCGCCTCCAC CGCCAGCTAC TTACCCCGAA 
TCCTACGACC CATACGACCC CCAACCGGAC CTGCCGCTCC CGCCGCCTCC CTCCGCCGTC 
ACTCCTGCGG TAGCTCAGCA GGCGGCCGAC CGCAAGAAAC GTGAGGAGCA GCAGCGCTGG 
TATGAGAAGG AGAAAGCTCG GCTGGAGGAG GAGAGGGAGA GGAAGAGGAG AGAGCAGGAG 
AGGAAGCTGG GTCAGATCCG GGCCAACCCC GTCATGCCCG TCCAGCCTCA CAACAACGGA 
GGATCCATGC CTCCGCCTCA CCACCAGCCG CCTCTGCCGT CTGCAGCCCC GCCTCAGCCC 
TACCTTCCCC CCGCTCAGCC CCAGCCTCCA CCATCCAGAC CGGAGAAGCT GACCAGCCTG 
CCGCGGTCAG CCGTTCCTCC ACCCGCCAGC GCCTCCAACC CTGCAGGCAT GGACACGGTG 
ATCAGGGAGC TGTTGCCACA GCAGCAGCCG CGGACCATCG AGAGGCGAGA CCTGCAGTAC 
ATCACCATCA GCAAGGAGGA GCTCACCTCC AGCGACAGTC TGTCTCCAGA CCCCTGGAAG 
CGAGACGCCC GCGAGAAGGC CGAGAAGCAG CAGCAGCTCC ACATCGTCGA CCTGCTGGAC 
AAGGAGATCC AGGAGCTCCA GGCGAAGCCG GAGCGGACGG CGGAGGAGAG CGACCGCCTC 
AGGAAGCTGA TGCTGGAGTG GCAGTTCCAG AAACGACTGC AGGAGTCCAA GCAGAGCGAC 
GAAGACGAGG AGGAGGAGGA CGACGAGGAC GTGGACACCA TGATGATCAT GCAGAGGCTG 
GAGGCCGAGA AGAGAGCCAG GCAGCAGAAT GCTGTCCCAG CTATCTCTGT TCTAGACTTG 
TTGCAGGACG AGGAGCGGCG GCGTAAGCAG CAGCTGGAGG AGATTCGTAA GCGGGAGGCG 
GAGGAGCGAG CAAAGCAGGA GGAGGAGAGG AGGTGGAGGG AGGAGGAGAG AGCCAAGCGA 
GAGGCCGAGG AGAAGAGGAG ACAGGAGGAG GAGTACTACA CTCGCCTGGA GGCGGAGCGG 
CGCCGGCAGC ACGACGAGGC CGAGAGGAAG CTGCTGACGC CCGACGACCC GGCAGGATTT 
AACTCGTACA CTGGAAACTC AGCAGGAGTT GTAGGATCAG GAGAAGTCTA CAAGGACCCG 
CGAGACAAAT GGACGAAGAG TCAGGAGCAG GAGAACTCGA ACCCCAGCGG CCCTCCGGAG 
AATCTGACCT TCAAGGAGCG GCAGCGTCTC TTCTCTCAGG GGAAGGAGGT TTCCAACAAG 
GTCAAAGCTT CGCGCAAACT CATGGAGCTG GAGAACGAGC TCAACACCAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019121279.1
CDS	228..5861
Translation	MSGSREEERRKLADIINHWNANRLDLFEISRPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERQLDLDEKPLVVQLNWNKDDREGRFVLKNENDILPKKSQSNGPEKEKDGVIQNFKRTLSKKEKKKEKKREKEFARIPDGDEQALSREDGENSRLAAEVYKDMPENSFTRTISNPEVVMKRRRQQKLEKRMQEFMSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTRDTADFAVVEALEKYGLEKENPREYCIARQDDKSGKEAILDDTECPLQIFRDWPADRGALVFQLKKRPPDYQGRKGRKVDDKGLRGKDGSSSLPPEKLPYLVELSPGRGNHYAYYAYRHHEDGSDSRDKPKLYRLQHSITEVGSDCTEDGAIQLLGPGILPHHCNLMHSEGMVTVTPHGPDADTFVDGQRVTETTVLRSGSTLQFGQAHVFKFVDPMFDQGGKREPAAMMRSRHKSGSVPETTFDLHGDVHSGAALPTSKSSGKLEMERGMVKPMIRGEQQDGRSQDISADRAELTLPAGIEFRDNCDSVPETTFDLHGDVHSGAALPTSKSSGKLEMERGMVKPMIRGEQQDGRSQDISADRAELTLPAGIEFRDNSEDTFLSAIINYTNSSTVHFKLSPTYVLYMACRYILSPSYRPDMSPSERTHKVIAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLNRITLDAQDILAHLVQMAFKYLVHCLQADLNNYMPAFLDDPEEHNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVTDTESGLCCHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVPNIHNTCFKLNSLQLHALMNNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRSLPNGLQDFLEPLLQRGFCRLVPHPRSPGTWTVHFEGADCDSHFSAADNSEMPMRKEPEVVTVTLKKHNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASDRGRDKNGKLRPKSEGFELYNSSVQNGSPESPQAGWDSYPEPKKMSGEDRLLKNRADHRSSPNVANQGQSPAGKAVYPGGPGTKITSVSTGNLCADDEPSPPRPEAYPIPTQTYPREYFTIPASKSQDRVVGPGQGQHWQGMEDRERLPVVDNIHNSMQRVNHSQEELYPPPGGLRPEERMQQHYQQDYQHRDMDYQHRELDYQRGGPPGVGGPDHWTPQPQVSSSLESSTSSQEHLNFSASSSSSNKTQNQKTGPGRWKTPNAPHSVPPHSAQPPSRSDLPPPPPPPPATYPESYDPYDPQPDLPLPPPPSAVTPAVAQQAADRKKREEQQRWYEKEKARLEEERERKRREQERKLGQIRANPVMPVQPHNNGGSMPPPHHQPPLPSAAPPQPYLPPAQPQPPPSRPEKLTSLPRSAVPPPASASNPAGMDTVIRELLPQQQPRTIERRDLQYITISKEELTSSDSLSPDPWKRDAREKAEKQQQLHIVDLLDKEIQELQAKPERTAEESDRLRKLMLEWQFQKRLQESKQSDEDEEEEDDEDVDTMMIMQRLEAEKRARQQNAVPAISVLDLLQDEERRRKQQLEEIRKREAEERAKQEEERRWREEERAKREAEEKRRQEEEYYTRLEAERRRQHDEAERKLLTPDDPAGFNSYTGNSAGVVGSGEVYKDPRDKWTKSQEQENSNPSGPPENLTFKERQRLFSQGKEVSNKVKASRKLMELENELNTK

Target ORF information:

RefSeq Version	XM_019265734.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea afadin, adherens junction formation factor (afdn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019265734.1

ORF Insert Sequence:

ATGTCGGGCA GCAGGGAGGA GGAGAGGAGG AAACTGGCCG ACATCATCAA CCACTGGAAC 
GCCAACCGGC TCGACCTGTT CGAGATCAGC CGGCCCACAG AGGACCTGGA GTTCCATGGC 
GTGATGCGGT TCTACTTCCA GGACCGGGTC GCCGGTAACT TTGCCACCAA ATGCATCAGA 
GTGTCGAGCA CGGCGACAAC GCAGGACGTC ATCGAGACGT TAGCAGAGAA GTTCAGACCG 
GACATGAGGA TGCTCTCCTC GCCAAAATAC TCTTTGTACG AGGTCCACGT CAGTGGCGAG 
CGTCAGCTGG ATCTGGACGA GAAGCCTCTG GTGGTTCAGC TGAACTGGAA CAAAGACGAC 
AGAGAAGGAC GCTTCGTCCT GAAGAACGAG AACGACATCC TCCCCAAGAA GAGTCAGAGT 
AACGGCCCGG AGAAGGAGAA GGACGGAGTG ATCCAGAACT TCAAGAGGAC TTTGTCCAAG 
AAGGAGAAGA AGAAGGAGAA GAAGAGGGAG AAGGAGTTCG CCCGGATCCC CGACGGAGAC 
GAGCAGGCGC TCAGCCGAGA GGACGGGGAG AACAGTCGTC TGGCGGCGGA GGTTTACAAG 
GACATGCCGG AGAACAGCTT CACCAGGACG ATCTCCAACC CGGAGGTGGT GATGAAGAGG 
AGACGCCAGC AGAAGCTGGA GAAACGCATG CAGGAGTTCA TGAGCAGCGA CGGGAGGCCC 
GACTCAGGGG GCACGTTGCG GATATACGCC GACAGCCTGA AGCCGAACAT CCCGTATAAA 
ACCATCCTGC TGTCCACGAG AGACACGGCT GACTTTGCGG TGGTGGAGGC GCTGGAGAAG 
TACGGGCTGG AGAAGGAGAA CCCCCGAGAG TACTGCATCG CCCGGCAAGA CGATAAGTCC 
GGCAAGGAGG CGATCCTGGA CGATACCGAG TGTCCACTGC AGATCTTCAG GGACTGGCCT 
GCAGACAGAG GAGCGCTAGT GTTCCAGCTG AAGAAGAGAC CCCCGGACTA CCAGGGCAGG 
AAGGGGAGGA AAGTGGACGA CAAGGGCCTG CGAGGGAAGG ACGGCAGCAG CTCCCTGCCG 
CCCGAGAAAC TGCCTTACCT GGTGGAGCTG AGCCCAGGGC GAGGGAATCA CTATGCCTAC 
TACGCCTATC GGCACCACGA AGACGGGTCT GACTCACGGG ACAAACCAAA GCTGTACCGG 
CTCCAGCACA GCATCACTGA GGTCGGCTCA GACTGCACAG AGGACGGAGC CATTCAGCTG 
CTCGGTCCGG GGATTCTGCC CCACCACTGC AACCTCATGC ACAGTGAAGG CATGGTGACG 
GTGACGCCGC ACGGCCCCGA CGCCGACACC TTCGTCGACG GGCAACGGGT CACTGAGACG 
ACGGTGCTGC GCTCGGGCTC CACCCTCCAG TTTGGCCAAG CGCACGTCTT CAAGTTTGTG 
GACCCGATGT TCGACCAGGG AGGGAAGAGA GAGCCGGCCG CCATGATGAG GAGTCGACAC 
AAGTCCGGCA GCGTACCCGA AACCACCTTC GACCTTCACG GAGACGTCCA CAGCGGAGCC 
GCGCTGCCTA CCAGCAAGAG CTCAGGAAAG CTGGAGATGG AGAGAGGAAT GGTCAAACCG 
ATGATCAGAG GAGAGCAGCA GGACGGCAGG TCTCAGGACA TTTCAGCAGA CAGAGCCGAG 
TTGACTCTGC CAGCCGGCAT CGAGTTCAGA GACAACTGTG ACAGCGTACC CGAAACCACC 
TTCGACCTTC ACGGAGACGT CCACAGCGGA GCCGCGCTGC CTACCAGCAA GAGCTCAGGA 
AAGCTGGAGA TGGAGAGAGG AATGGTCAAA CCGATGATCA GAGGAGAGCA GCAGGACGGC 
AGGTCTCAGG ACATTTCAGC AGACAGAGCC GAGTTGACTC TGCCAGCCGG CATCGAGTTC 
AGAGACAACT CTGAAGACAC CTTCCTGTCG GCCATCATCA ACTACACCAA CAGCTCCACG 
GTTCACTTCA AACTCTCGCC CACGTACGTC CTGTACATGG CCTGCCGCTA CATCCTGTCG 
CCCTCGTACC GGCCCGACAT GTCGCCGTCC GAGAGGACGC ACAAGGTCAT CGCCATCGTC 
AACAAGATGG TGAGCATGAT GGAGGGAGTC ATCCAGAAGC AGAAGAACAT CGCGGGAGCG 
CTGGCTTTCT GGATGGCCAA CGCCTCCGAG CTGCTCAACT TCATCAAGCA GGACCGAGAC 
CTGAACCGCA TCACGCTGGA CGCCCAGGAC ATCCTCGCCC ATCTGGTTCA GATGGCTTTC 
AAGTACCTGG TCCACTGTCT GCAGGCTGAT CTGAATAACT ACATGCCGGC CTTCCTGGAC 
GACCCCGAGG AACACAACCC ACAGAGACCC AAGATCGAGG ACGTCCTGCA CACCCTCACG 
GGGGCGATGT CGTTACTGCG GCGGTGTCGG GTCAACGCCG CTCTCACCAT CCAGCTCTTC 
TCGCAGCTCT TCCACTTCAT CAACATGTGG CTCTTCAACA AGCTGGTGAC GGACACCGAG 
TCCGGGCTGT GCTGTCACTA CTGGGGGGCC ATCCTGCGGC AGCAGCTCAG CCACATCGAG 
GCCTGGGCGG AGAAACAGGG GCTGGAGCTC GCCGCCGACT GCCACCTCAG TCGAATCGTG 
CAGGCCACCA CCCTGCTGAC CATGGACAAA TACTCCATGC AGGATGTGCC GAACATCCAC 
AACACCTGCT TCAAGCTCAA CTCGCTGCAG CTCCACGCCC TCATGAACAA CTACCACTGC 
GCTCCGGACG AGCCGTACAT CCCGCCGGAG CTGATAGACC ACGTGGTGGC AGTGGCAGAG 
AACACAGCAG ATGAGCTGGC GAGGAGCGAC GGCAGAGAGG TTCAGCTGGA GGAGGATCCG 
GACCTCCAGC TGCCGTTCCT CCTTCCCGAG GACGGTTACT CCTGCGACGT GGTGCGCAGC 
CTCCCCAACG GCCTGCAGGA CTTCCTGGAG CCGCTGCTGC AGAGAGGTTT CTGTCGGTTG 
GTACCTCACC CTCGCTCTCC CGGTACCTGG ACGGTCCATT TTGAAGGAGC TGACTGCGAC 
AGCCACTTCT CTGCTGCTGA CAACTCAGAG ATGCCAATGA GGAAAGAGCC GGAGGTTGTC 
ACGGTAACCC TGAAGAAGCA CAACGGCATG GGCCTCAGCA TCGTGGCTGC CAAGGGTGCT 
GGTCAGGAGA AGCTGGGCAT CTACATCAAG TCTGTCGTCA AAGGAGGAGC AGCTGATGTG 
GACGGGCGTC TGGCTGCAGG TGACCAGCTG TTGAGCGTGG ACGGGCGGAG TCTGGTCGGC 
CTATCACAAG AGAGGGCAGC GGAGCTGATG ACGAGGACGG GCTCAGTCGT GACTCTGGAG 
GTCGCCAAGC AGGGAGCCAT TTACCACGGA CTGGCCACGC TCCTCAACCA GCCGAGCCCC 
ATGATGCCAC GAGCGTCAGA CAGAGGCCGC GACAAGAACG GGAAACTCCG ACCAAAGAGC 
GAAGGCTTCG AGTTGTACAA CAGCTCGGTG CAGAACGGCT CTCCGGAGAG CCCGCAGGCC 
GGCTGGGACT CTTACCCCGA ACCAAAGAAG ATGAGCGGCG AAGATCGACT CCTGAAGAAC 
CGAGCCGACC ACCGCTCCAG CCCCAACGTC GCCAATCAGG GCCAGAGTCC TGCAGGCAAA 
GCAGTGTACC CCGGAGGACC TGGCACCAAG ATCACCTCCG TCTCCACCGG CAACCTGTGC 
GCAGACGATG AGCCCTCCCC TCCACGTCCA GAAGCTTACC CCATCCCCAC CCAGACCTAC 
CCCAGGGAGT ACTTCACCAT CCCGGCTTCC AAGAGCCAGG ATCGGGTCGT TGGTCCTGGG 
CAGGGACAGC ACTGGCAGGG CATGGAGGAC AGAGAACGCC TCCCTGTGGT CGACAACATC 
CACAACAGCA TGCAGCGGGT TAACCACTCC CAGGAGGAGT TGTACCCTCC ACCCGGAGGC 
CTGAGGCCGG AGGAGCGCAT GCAGCAGCAC TACCAGCAGG ACTACCAGCA CCGCGACATG 
GACTACCAGC ACAGAGAGCT CGACTACCAG AGAGGAGGTC CACCAGGTGT CGGAGGTCCC 
GATCACTGGA CTCCACAGCC TCAGGTGTCC TCCTCGCTGG AGTCGTCCAC GTCCAGCCAG 
GAGCACCTCA ACTTCTCCGC CTCCTCCTCG TCCTCCAACA AAACCCAGAA CCAGAAGACC 
GGGCCGGGCC GGTGGAAGAC TCCCAACGCC CCTCACTCCG TGCCGCCACA TTCAGCCCAG 
CCGCCCTCCC GCTCCGACCT GCCCCCTCCT CCGCCTCCAC CGCCAGCTAC TTACCCCGAA 
TCCTACGACC CATACGACCC CCAACCGGAC CTGCCGCTCC CGCCGCCTCC CTCCGCCGTC 
ACTCCTGCGG TAGCTCAGCA GGCGGCCGAC CGCAAGAAAC GTGAGGAGCA GCAGCGCTGG 
TATGAGAAGG AGAAAGCTCG GCTGGAGGAG GAGAGGGAGA GGAAGAGGAG AGAGCAGGAG 
AGGAAGCTGG GTCAGATCCG GGCCAACCCC GTCATGCCCG TCCAGCCTCA CAACAACGGA 
GGATCCATGC CTCCGCCTCA CCACCAGCCG CCTCTGCCGT CTGCAGCCCC GCCTCAGCCC 
TACCTTCCCC CCGCTCAGCC CCAGCCTCCA CCATCCAGAC CGGAGAAGCT GACCAGCCTG 
CCGCGGTCAG CCGTTCCTCC ACCCGCCAGC GCCTCCAACC CTGCAGGCAT GGACACGGTG 
ATCAGGGAGC TGTTGCCACA GCAGCAGCCG CGGACCATCG AGAGGCGAGA CCTGCAGTAC 
ATCACCATCA GCAAGGAGGA GCTCACCTCC AGCGACAGTC TGTCTCCAGA CCCCTGGAAG 
CGAGACGCCC GCGAGAAGGC CGAGAAGCAG CAGCAGCTCC ACATCGTCGA CCTGCTGGAC 
AAGGAGATCC AGGAGCTCCA GGCGAAGCCG GAGCGGACGG CGGAGGAGAG CGACCGCCTC 
AGGAAGCTGA TGCTGGAGTG GCAGTTCCAG AAACGACTGC AGGAGTCCAA GCAGAGCGAC 
GAAGACGAGG AGGAGGAGGA CGACGAGGAC GTGGACACCA TGATGATCAT GCAGAGGCTG 
GAGGCCGAGA AGAGAGCCAG GCAGCAGAAT GCTGTCCCAG CTATCTCTGT TCTAGACTTG 
TTGCAGGACG AGGAGCGGCG GCGTAAGCAG CAGCTGGAGG AGATTCGTAA GCGGGAGGCG 
GAGGAGCGAG CAAAGCAGGA GGAGGAGAGG AGGTGGAGGG AGGAGGAGAG AGCCAAGCGA 
GAGGCCGAGG AGAAGAGGAG ACAGGAGGAG GAGTACTACA CTCGCCTGGA GGCGGAGCGG 
CGCCGGCAGC ACGACGAGGC CGAGAGGAAG CTGCTGACGC CCGACGACCC GGCAGGATTT 
AACTCGTACA CTGGAAACTC AGCAGGAGTT GTAGGATCAG GAGAAGTCTA CAAGGACCCG 
CGAGACAAAT GGACGAAGAG TCAGGAGCAG GAGAACTCGA ACCCCAGCGG CCCTCCGGAG 
AATCTGACCT TCAAGGAGCG GCAGCGTCTC TTCTCTCAGG GGAAGGAGGT TTCCAACAAG 
GTCAAAGCTT CGCGCAAACT CATGGAGCTG GAGAACGAGC TCAACACCAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	afdn
Entrez Gene ID	104934235
Full Name	afadin, adherens junction formation factor
Gene Type	protein-coding
Organism	Larimichthys crocea(large yellow croaker)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

afdn cDNA ORF clone, Larimichthys crocea(large yellow croaker)

Gene

Clones

gRNAs

Target ORF information:

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