LOC104931439 gene cDNA ORF clone, Larimichthys crocea(large yellow croaker)

mRNA	Protein	Name
XM_019263543.1	XP_019119088.1	receptor-type tyrosine-protein phosphatase F isoform X5
XM_010746447.2	XP_010744749.2	receptor-type tyrosine-protein phosphatase F isoform X6
XM_010746451.2	XP_010744753.2	receptor-type tyrosine-protein phosphatase F isoform X9
XM_010746454.2	XP_010744756.2	receptor-type tyrosine-protein phosphatase F isoform X13
XM_019263528.2	XP_019119073.1	receptor-type tyrosine-protein phosphatase F isoform X1
XM_010746444.3	XP_010744746.2	receptor-type tyrosine-protein phosphatase F isoform X3
XM_010746450.3	XP_010744752.2	receptor-type tyrosine-protein phosphatase F isoform X11
XM_019263588.2	XP_019119133.1	receptor-type tyrosine-protein phosphatase F isoform X14
XM_019263593.2	XP_019119138.1	receptor-type tyrosine-protein phosphatase F isoform X15
XM_019263528.1	XP_019119073.1	receptor-type tyrosine-protein phosphatase F isoform X1
XM_010746444.2	XP_010744746.2	receptor-type tyrosine-protein phosphatase F isoform X3
XM_019263564.1	XP_019119109.1	receptor-type tyrosine-protein phosphatase F isoform X8
XM_010746450.2	XP_010744752.2	receptor-type tyrosine-protein phosphatase F isoform X11
XM_019263532.2	XP_019119077.1	receptor-type tyrosine-protein phosphatase F isoform X2
XM_019263564.2	XP_019119109.1	receptor-type tyrosine-protein phosphatase F isoform X8
XM_010746451.3	XP_010744753.2	receptor-type tyrosine-protein phosphatase F isoform X9
XM_010746452.3	XP_010744754.2	receptor-type tyrosine-protein phosphatase F isoform X10
XM_019263532.1	XP_019119077.1	receptor-type tyrosine-protein phosphatase F isoform X2
XM_019263593.1	XP_019119138.1	receptor-type tyrosine-protein phosphatase F isoform X15
XM_019263523.2	XP_019119068.1	receptor-type tyrosine-protein phosphatase F isoform X1
XM_019263543.2	XP_019119088.1	receptor-type tyrosine-protein phosphatase F isoform X5
XM_010746453.3	XP_010744755.2	receptor-type tyrosine-protein phosphatase F isoform X12
XM_019263557.1	XP_019119102.1	receptor-type tyrosine-protein phosphatase F isoform X7
XM_019263539.2	XP_019119084.1	receptor-type tyrosine-protein phosphatase F isoform X4
XM_010746454.3	XP_010744756.2	receptor-type tyrosine-protein phosphatase F isoform X13
XM_019263557.2	XP_019119102.1	receptor-type tyrosine-protein phosphatase F isoform X7
XM_019263523.1	XP_019119068.1	receptor-type tyrosine-protein phosphatase F isoform X1
XM_019263539.1	XP_019119084.1	receptor-type tyrosine-protein phosphatase F isoform X4
XM_010746452.2	XP_010744754.2	receptor-type tyrosine-protein phosphatase F isoform X10
XM_010746453.2	XP_010744755.2	receptor-type tyrosine-protein phosphatase F isoform X12
XM_019263588.1	XP_019119133.1	receptor-type tyrosine-protein phosphatase F isoform X14
XM_010746447.3	XP_010744749.2	receptor-type tyrosine-protein phosphatase F isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OLa211346	XM_019263523.2 Latest version!	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa211346	XM_019263528.2 Latest version!	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa211347	XM_019263532.2 Latest version!	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa211348	XM_010746444.3 Latest version!	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa211349	XM_019263539.2 Latest version!	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa211350	XM_019263543.2 Latest version!	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa211351	XM_010746447.3 Latest version!	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa211352	XM_019263557.2 Latest version!	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa211353	XM_019263564.2 Latest version!	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa211354	XM_010746451.3 Latest version!	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa211355	XM_010746452.3 Latest version!	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa211356	XM_010746450.3 Latest version!	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa211357	XM_010746453.3 Latest version!	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa211358	XM_010746454.3 Latest version!	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa211359	XM_019263588.2 Latest version!	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa211360	XM_019263593.2 Latest version!	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa84596	XM_019263528.1 Latest version!	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OLa84596	XM_019263523.1 Latest version!	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OLa84597	XM_019263532.1 Latest version!	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OLa84598	XM_010746444.2 Latest version!	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OLa84599	XM_019263539.1 Latest version!	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OLa84600	XM_019263543.1 Latest version!	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OLa84601	XM_010746447.2 Latest version!	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OLa84602	XM_019263557.1 Latest version!	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OLa84603	XM_019263564.1 Latest version!	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OLa84604	XM_010746451.2 Latest version!	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OLa84605	XM_010746452.2 Latest version!	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OLa84606	XM_010746450.2 Latest version!	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OLa84607	XM_010746453.2 Latest version!	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OLa84608	XM_010746454.2 Latest version!	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OLa84609	XM_019263588.1 Latest version!	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OLa84610	XM_019263593.1 Latest version!	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

LOC104931439 ( XM_019263528.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019119073.1 Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OLa211346
Clone ID Related Accession (Same CDS sequence)	XM_019263528.2 , XM_019263523.2
Accession Version	XM_019263528.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	receptor-type tyrosine-protein phosphatase F isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040012.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_019263528.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTGGACATC 
AGCAGCAGCA ACGGGCGCAT TAAACAGCTT CGTTCAGGTG GTACACCAAT TAGAGGAGCT 
CTGCAAATTG AGAACAGCGA GGAGTCCGAC CAAGGGAAAT ACGAGTGTGT TGCCATGAAC 
AGCGCTGGGA CCCGTTACTC CGCTCCTGCC AATCTCTACG TACGAGTGCG AAGAGTCCCA 
CCCAGATTCT CCATTCCGCC CACCAACCAA GAGGTGATGC CAGGCGGCAG CGTCAACCTG 
ACATGCGTGG CAGTGGGCTC GCCGATGCCC TACGTCAAGT GGACGATGGG CCTGGTGGAG 
CTGACCAAGG AGGAGGAGAT GCCGATGGGA CGCAACGTGC TGGAGGTCAA CAACATCCGC 
GAGTCGGCGA ACTACACCTG CGTGGCCATC TCCTCTCTGG GCATGATAGA AGCCACCGCT 
CAGGTCACCG TCAAAGCTTT GCCAAAGCCT CCCGCCTCCC TCATTGTGAC TGAGACCACA 
GCCACCAGTG TGACTCTCAC CTGGGACTCT GGAAACCCAG AACCTGTCTC CTACTACATG 
ATCCAATACA GAGCCAAGAC CTCGGACAAC GGCTTCCAGG AAGTGGAAGG TGTGGCAACT 
ACCCGTTACA GCATCGGTGG ACTTAGCCCC TACTCTGAGT ATGAGTTTCG TGTCATGGCT 
GTCAACAACA TCGGCCGGGG GCCACCGAGC GACCCCGTGG AGACACGCAC TGGTGAACAG 
GCCCCCTCCT CGCCACCTCT GCACGTCCAA GCGCGCATGC TGAGTGCCAG CACAATGCTG 
GTCCAGTGGG AACCGCCCGA GGAACCCAAC GGGCAAATCA GGGGCTACCG GGTCTACTAC 
AGCTCGGACT TGACGGCTCC GCTCAGCGCC TGGCAGAAGC ACAACACAGA CGACAGCAGC 
CTGACGACCA TCTCGGGCCT GACTCCTGAT ATCACCTACA GCCTCAGGGT GCTGGGCTTC 
ACCTCTGTAG GTGACGGGCC ACCTTCTGAC ATCCTGCAAG TCAAAACCCA ACAGGGAGTC 
CCTGCCCAGC CGTCCAGCTT TGAGGCCGAA GCTGAGCTGG ACAGTCGCAT CATGCTAACA 
TGGCTGTGGC CCGTCCAGGA CCCCATCACT AAGTATGAGC TGCAGTACTG GGAGGCCGGC 
TCAGACAACA AGTTACATGT GAGCTTCAAC CCAGCGGGCT CCTACGCTGT GGACGGTCTA 
AAGCCTGACA CCTTGTACCG TTTCTCTCTG GCAGCCCGTT CTGAGATGGG CTTAGGTGTC 
TACACCCAAC CCATCGAGGC TCGCACCGCC CAGTCCACCC CATCAGCTCC CCCCCAGGAG 
GTACACCTGG TCAGCCTGAG CTCCACCAGC CTCAAGGTGA GTTGGGTGGC ACCGCCCGCC 
GCTAGCCGCC ACGGCGCCAT TGTGCGCTAC ACAGTCAGCT ACCAGGCCAT GGCTGGAGAA 
GACACGGAGC GGCACGAGGT GACGGGCATC GGCGCAGACG CCACTAGCTT TGTGCTGGAG 
GGCCTGGAGA AGTGGACTGA GTATCAGGTG TGGGTGAGGG CACACACTGA CGTGGGCCCC 
GGCCCAGAGA GTGCCCCAGT GAGGATGAGA ACCAAAGAGG ATGTGCCGGG AGCACCCCCC 
AGGAAGCTGG AGGTTGAGGC CATAAACTCC ACAGCAATCC GGGTCACCTG GAAGCCGCCC 
CTGCAGGGGA AGCAGCACGG CCAAATCCGG GGCTATCAAG TCATCTTCTC TCGGCTTGAA 
AACGGCGAAC CACGAGGCCA GCCTAACATC ATGGACGTTG CTTTGCCAGA GGCACAGTGG 
AATATTGAGG AGTCCACCGA GCATGAGGCC ATTATTGCCG GACTGCACTC TGAAACCACC 
TACTCGGTCA CCGTGGCAGC ATACACCACC AAAGGAGACG GAGCGCGCAG CAAAGCCAAA 
GTCATCACCA CGACGGGTGC AGTGCCAGGA AAGCCCACCA TGATGATCAG CACCACTATG 
GGCAACACAG CGCTGATTCA GTGGCAGCCG CCTAAGGAAA TGGTTGGGGA GCTGATGGGC 
TACCAGCTGC AGTACAAGCG CACTGACGAG GAAAACTACA CCTCCCGCGA GCTGAGAAAG 
ATCGATGACC ATTACACCGT CACCGGGCTG CACAAAGGTG CCACCTATGT CTTCCGCCTC 
AGTGCCCGCA ACCGCGCGGG CATCGGCGAA GCATACGTGA AAGAGATCAG CACCCCTGAA 
GACGTGCCGT CGGGCTTCCC GTTCAACCTG CAGGTCACGG GACTGACTCA GTCCAGCACC 
GAGCTGACCT GGGACCCTCC GCTGGTGGCC GAAAGGAACG GCAAGATCGT GCACTATGTG 
GTCGTTTACA GGGACATCAA CAGTCAGCAG AACAGCACCA ACCGGACGAG CGAAACGCAC 
ATGACCATCC AGGGCCTTAA TCCCGACACC ACCTACGATA TCCGGGTGCA GGCGTTCACC 
AGTAAGGGCG GAGGACCCCT CAGCCCTAGC ATCCAGAGCA GGACCATGTC GAACGATTTT 
CCCACCCTCA ACTTTGGTGT CAAAGCCGTC ATGAAAACAT CTGTCCTCCT CACCTGGGAT 
TTGCCACCGA ACTACAAATC CCAAGTGCCT TTCAAGATCC TGTACAACCA GCAAAGCGTG 
GAAGTGCAGG GATCCCTGAA GAGGAGGCTG ATCACGCAGC TGCAGCCAGA CACGGACTAC 
TCTTTTGTGC TGACAAGCAG GGGCAACATT GCTGGTGGTC TACAGCAGCA GGTGTCCATC 
CGCACCGCTC CCGACCTGAT CAACGCCAAG CCCAGCACAC ATCAGCCTCA GGGTAGCAAG 
ATGACAATCA ACCTGCCCAA GGTCCAGACC ACCACGCGTG TCAGGTGGTA CTACATCGTG 
GTGGTGCCGG TGTCCCAGTC AACGCAGGGG TGGAAGAATC CAGACGAGAT GGACCTGGAC 
GAGCTGTTCA AGTCAAGTGA AGGCCCCGTC CTGAGGCGCA GACGTCACCT GGATTCCTCC 
AAACCTTATA TCGCTGCCAA GTTGGCCTCG TTGCCGACCA CCTTCACCCT GGGTGATGAG 
AAGAGATACA ACGGCTTCTA CAACAAGCCC CTGAGCAGTC CGCAGGAGTA TCTCTGCTTT 
GTCCTGGCAG TACTCCGATC CGAAGGGGCA GACAGCACTA CTAATTATAT GACATTTTCA 
GCCAGTCCCT ACTCAGACCC GGTCCACGTG CGAAACAGCA TGGCTCAGGG GATGGAGCAG 
CCTGAAATGT TGTGGGTTAT GGGCCCTGTT TTGGCCGTGG TCCTGATTAT CATCATCGTC 
ATCGCCATCC TGCTCTTCAA GAACAAACAG GAAAGGAAGC GAGCGTCGCC CCTACCCAAA 
GATGAGCACT CGGGCGGGGT GAAGGACTCC CTGCTGGCCA ACTCCTCCGA CCCTGTGGAG 
ATGAGAAGAC TTAACTACCA GACCCCAGGT CCCAGCTCTC ATCGCTGCCC CAACACTCCA 
AGAATGAGGG AGCACCCGCC CATCCCTGTC GTCGACCTGG CTGACCACAT AGAGCGTCTG 
AAGGCCAACG ACGGCCTACG CTTTTCCCAG GAGTACGAGT CGATTGATCC AGGGCAGCAG 
TTTACCTGGG AGAACTCCAA CATGGAAGTC AACAAGCCAA AGAATCGCTA TGCAAACGTC 
ATCGCCTATG ACCACTCCAG AGTGGTTCTC ACCTCTGTTG ACGCCGTTCC AGGCAGTGAC 
TACATCAACG CCAACTACAT CGACGGCTAC AGGAAGCAGA ACGCCTACAT CGCCACGCAG 
GGGCCACTTC CAGAGACACT GAGCGACTTC TGGAGGATGG TGTGGGAGCA AAGATCCAAC 
ACCATCGTCA TGATGACCAG ACTGGAGGAG AAGTCGCGGG TGAAGTGTGA CCAGTACTGG 
CCGTCGCGAG GAACGGAGAC CTATGGGATG ATCCAGGTCA CCATGCTGGA CACCGTGGAG 
TTGGCCACTT ACAGCGTCCG TACCTTTGCC CTCTATAAGA ATGGCTCCAG TGAGAAGAGA 
GAGGTGAGAC AGTTCCAGTT CATGGCCTGG CCAGACCACG GGGTGCCAGA GTACCCCACC 
CCAATCCTGG CCTTCCTACG GCGGGTAAAG GCCTGCAATC CTCCAGACGC AGGGCCTATG 
GTGGTCCACT GCAGCGCGGG TGTGGGCAGG ACCGGATGCT TCATCGTGAT AGATGCCATG 
CTGGAGCGCA TGAAGCACGA GAAGTCCGTA GACATCTACG GCCACGTGAC GTGCATGCGC 
GCCCAGAGGA ACTACATGGT CCAGACCGAG GACCAGTACA TCTTCATTCA CGAAGCCCTG 
CTGGAGGCCG CCACGTGCGG CAACACCGAG GTGCCGGCGC GCAACCTGTA CGCCCACATC 
CAGAAACTCA CCCAGCCCCC GCCCGGCGAG ACGGTTACTG CCATGGAGCT GGAATTCAAG 
AGACTGGCCA ACTCCAAAGC CCACACATCC CGCTTCATCA GCGCCAACTT GCCCTGCAAC 
AAGTTCAAGA ACCGCCTGGT CAACATCATG CCTTTCGAGT CCACCCGCGT CTGCCTGCAG 
CCCATCCGTG GCGTCGAGGG CTCCGACTAC ATCAATGCCA GCTGCATCGA CGGCTACAGA 
CAGCAGAAGG CCTACCTGGC CACGCAGGGC CCACTGGCAG AGACCACCGA GGACTTCTGG 
AGGATGCTGT GGGAGCACAA CTCCACCATC GTCGTCATGC TCACCAAGCT ACGAGAGATG 
GGCCGGGAGA AGTGCCATCA GTACTGGCCA GCAGAGCGTT CAGCCAGGTA CCAGTACTTT 
GTGGTGGATC CAATGGCTGA GTACAACATG CCCCAGTACA TCCTCAGAGA GTTCAAAGTC 
ACAGATGCCA GGGACGGCCA GTCCAGAACA ATCCGCCAAT TCCAGTTCAC TGACTGGCCC 
GAGCAAGGAG TGCCAAAAAC CGGAGAGGGC TTCATTGATT TTATCGGACA AGTGCACAAA 
ACCAAGGAAC AGTTCGGACA GGACGGACCA ATCACTGTAC ACTGCAGTGC TGGGGTGGGC 
AGGACCGGAG TCTTCATCAC CCTCAGCATC GTGCTGGAGA GGATGAGGTA TGAAGGAGTT 
GTCGACCTCT TCCAGACAGT GAAGACACTT CGCACCCAGC GGCCTGCCAT GGTGCAGACC 
GAGGACCAGT ACCAGTTGTG CTACAGGGCA GCATTGGAGT ACCTGGGGAG CTTTGACCAC 
TATGCAACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019119073.1
CDS	746..6517
Translation	MVPNIHLAVPFLPRGALLFLILLSCVVFSSLADSMPSFVKSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVEKTRTATMLCAASGNPDPEISWFKDMLPVDISSSNGRIKQLRSGGTPIRGALQIENSEESDQGKYECVAMNSAGTRYSAPANLYVRVRRVPPRFSIPPTNQEVMPGGSVNLTCVAVGSPMPYVKWTMGLVELTKEEEMPMGRNVLEVNNIRESANYTCVAISSLGMIEATAQVTVKALPKPPASLIVTETTATSVTLTWDSGNPEPVSYYMIQYRAKTSDNGFQEVEGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSDPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRVYYSSDLTAPLSAWQKHNTDDSSLTTISGLTPDITYSLRVLGFTSVGDGPPSDILQVKTQQGVPAQPSSFEAEAELDSRIMLTWLWPVQDPITKYELQYWEAGSDNKLHVSFNPAGSYAVDGLKPDTLYRFSLAARSEMGLGVYTQPIEARTAQSTPSAPPQEVHLVSLSSTSLKVSWVAPPAASRHGAIVRYTVSYQAMAGEDTERHEVTGIGADATSFVLEGLEKWTEYQVWVRAHTDVGPGPESAPVRMRTKEDVPGAPPRKLEVEAINSTAIRVTWKPPLQGKQHGQIRGYQVIFSRLENGEPRGQPNIMDVALPEAQWNIEESTEHEAIIAGLHSETTYSVTVAAYTTKGDGARSKAKVITTTGAVPGKPTMMISTTMGNTALIQWQPPKEMVGELMGYQLQYKRTDEENYTSRELRKIDDHYTVTGLHKGATYVFRLSARNRAGIGEAYVKEISTPEDVPSGFPFNLQVTGLTQSSTELTWDPPLVAERNGKIVHYVVVYRDINSQQNSTNRTSETHMTIQGLNPDTTYDIRVQAFTSKGGGPLSPSIQSRTMSNDFPTLNFGVKAVMKTSVLLTWDLPPNYKSQVPFKILYNQQSVEVQGSLKRRLITQLQPDTDYSFVLTSRGNIAGGLQQQVSIRTAPDLINAKPSTHQPQGSKMTINLPKVQTTTRVRWYYIVVVPVSQSTQGWKNPDEMDLDELFKSSEGPVLRRRRHLDSSKPYIAAKLASLPTTFTLGDEKRYNGFYNKPLSSPQEYLCFVLAVLRSEGADSTTNYMTFSASPYSDPVHVRNSMAQGMEQPEMLWVMGPVLAVVLIIIIVIAILLFKNKQERKRASPLPKDEHSGGVKDSLLANSSDPVEMRRLNYQTPGPSSHRCPNTPRMREHPPIPVVDLADHIERLKANDGLRFSQEYESIDPGQQFTWENSNMEVNKPKNRYANVIAYDHSRVVLTSVDAVPGSDYINANYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRSNTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVTMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRAQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPPPGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYLATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_019263528.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019263528.2

ORF Insert Sequence:

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTGGACATC 
AGCAGCAGCA ACGGGCGCAT TAAACAGCTT CGTTCAGGTG GTACACCAAT TAGAGGAGCT 
CTGCAAATTG AGAACAGCGA GGAGTCCGAC CAAGGGAAAT ACGAGTGTGT TGCCATGAAC 
AGCGCTGGGA CCCGTTACTC CGCTCCTGCC AATCTCTACG TACGAGTGCG AAGAGTCCCA 
CCCAGATTCT CCATTCCGCC CACCAACCAA GAGGTGATGC CAGGCGGCAG CGTCAACCTG 
ACATGCGTGG CAGTGGGCTC GCCGATGCCC TACGTCAAGT GGACGATGGG CCTGGTGGAG 
CTGACCAAGG AGGAGGAGAT GCCGATGGGA CGCAACGTGC TGGAGGTCAA CAACATCCGC 
GAGTCGGCGA ACTACACCTG CGTGGCCATC TCCTCTCTGG GCATGATAGA AGCCACCGCT 
CAGGTCACCG TCAAAGCTTT GCCAAAGCCT CCCGCCTCCC TCATTGTGAC TGAGACCACA 
GCCACCAGTG TGACTCTCAC CTGGGACTCT GGAAACCCAG AACCTGTCTC CTACTACATG 
ATCCAATACA GAGCCAAGAC CTCGGACAAC GGCTTCCAGG AAGTGGAAGG TGTGGCAACT 
ACCCGTTACA GCATCGGTGG ACTTAGCCCC TACTCTGAGT ATGAGTTTCG TGTCATGGCT 
GTCAACAACA TCGGCCGGGG GCCACCGAGC GACCCCGTGG AGACACGCAC TGGTGAACAG 
GCCCCCTCCT CGCCACCTCT GCACGTCCAA GCGCGCATGC TGAGTGCCAG CACAATGCTG 
GTCCAGTGGG AACCGCCCGA GGAACCCAAC GGGCAAATCA GGGGCTACCG GGTCTACTAC 
AGCTCGGACT TGACGGCTCC GCTCAGCGCC TGGCAGAAGC ACAACACAGA CGACAGCAGC 
CTGACGACCA TCTCGGGCCT GACTCCTGAT ATCACCTACA GCCTCAGGGT GCTGGGCTTC 
ACCTCTGTAG GTGACGGGCC ACCTTCTGAC ATCCTGCAAG TCAAAACCCA ACAGGGAGTC 
CCTGCCCAGC CGTCCAGCTT TGAGGCCGAA GCTGAGCTGG ACAGTCGCAT CATGCTAACA 
TGGCTGTGGC CCGTCCAGGA CCCCATCACT AAGTATGAGC TGCAGTACTG GGAGGCCGGC 
TCAGACAACA AGTTACATGT GAGCTTCAAC CCAGCGGGCT CCTACGCTGT GGACGGTCTA 
AAGCCTGACA CCTTGTACCG TTTCTCTCTG GCAGCCCGTT CTGAGATGGG CTTAGGTGTC 
TACACCCAAC CCATCGAGGC TCGCACCGCC CAGTCCACCC CATCAGCTCC CCCCCAGGAG 
GTACACCTGG TCAGCCTGAG CTCCACCAGC CTCAAGGTGA GTTGGGTGGC ACCGCCCGCC 
GCTAGCCGCC ACGGCGCCAT TGTGCGCTAC ACAGTCAGCT ACCAGGCCAT GGCTGGAGAA 
GACACGGAGC GGCACGAGGT GACGGGCATC GGCGCAGACG CCACTAGCTT TGTGCTGGAG 
GGCCTGGAGA AGTGGACTGA GTATCAGGTG TGGGTGAGGG CACACACTGA CGTGGGCCCC 
GGCCCAGAGA GTGCCCCAGT GAGGATGAGA ACCAAAGAGG ATGTGCCGGG AGCACCCCCC 
AGGAAGCTGG AGGTTGAGGC CATAAACTCC ACAGCAATCC GGGTCACCTG GAAGCCGCCC 
CTGCAGGGGA AGCAGCACGG CCAAATCCGG GGCTATCAAG TCATCTTCTC TCGGCTTGAA 
AACGGCGAAC CACGAGGCCA GCCTAACATC ATGGACGTTG CTTTGCCAGA GGCACAGTGG 
AATATTGAGG AGTCCACCGA GCATGAGGCC ATTATTGCCG GACTGCACTC TGAAACCACC 
TACTCGGTCA CCGTGGCAGC ATACACCACC AAAGGAGACG GAGCGCGCAG CAAAGCCAAA 
GTCATCACCA CGACGGGTGC AGTGCCAGGA AAGCCCACCA TGATGATCAG CACCACTATG 
GGCAACACAG CGCTGATTCA GTGGCAGCCG CCTAAGGAAA TGGTTGGGGA GCTGATGGGC 
TACCAGCTGC AGTACAAGCG CACTGACGAG GAAAACTACA CCTCCCGCGA GCTGAGAAAG 
ATCGATGACC ATTACACCGT CACCGGGCTG CACAAAGGTG CCACCTATGT CTTCCGCCTC 
AGTGCCCGCA ACCGCGCGGG CATCGGCGAA GCATACGTGA AAGAGATCAG CACCCCTGAA 
GACGTGCCGT CGGGCTTCCC GTTCAACCTG CAGGTCACGG GACTGACTCA GTCCAGCACC 
GAGCTGACCT GGGACCCTCC GCTGGTGGCC GAAAGGAACG GCAAGATCGT GCACTATGTG 
GTCGTTTACA GGGACATCAA CAGTCAGCAG AACAGCACCA ACCGGACGAG CGAAACGCAC 
ATGACCATCC AGGGCCTTAA TCCCGACACC ACCTACGATA TCCGGGTGCA GGCGTTCACC 
AGTAAGGGCG GAGGACCCCT CAGCCCTAGC ATCCAGAGCA GGACCATGTC GAACGATTTT 
CCCACCCTCA ACTTTGGTGT CAAAGCCGTC ATGAAAACAT CTGTCCTCCT CACCTGGGAT 
TTGCCACCGA ACTACAAATC CCAAGTGCCT TTCAAGATCC TGTACAACCA GCAAAGCGTG 
GAAGTGCAGG GATCCCTGAA GAGGAGGCTG ATCACGCAGC TGCAGCCAGA CACGGACTAC 
TCTTTTGTGC TGACAAGCAG GGGCAACATT GCTGGTGGTC TACAGCAGCA GGTGTCCATC 
CGCACCGCTC CCGACCTGAT CAACGCCAAG CCCAGCACAC ATCAGCCTCA GGGTAGCAAG 
ATGACAATCA ACCTGCCCAA GGTCCAGACC ACCACGCGTG TCAGGTGGTA CTACATCGTG 
GTGGTGCCGG TGTCCCAGTC AACGCAGGGG TGGAAGAATC CAGACGAGAT GGACCTGGAC 
GAGCTGTTCA AGTCAAGTGA AGGCCCCGTC CTGAGGCGCA GACGTCACCT GGATTCCTCC 
AAACCTTATA TCGCTGCCAA GTTGGCCTCG TTGCCGACCA CCTTCACCCT GGGTGATGAG 
AAGAGATACA ACGGCTTCTA CAACAAGCCC CTGAGCAGTC CGCAGGAGTA TCTCTGCTTT 
GTCCTGGCAG TACTCCGATC CGAAGGGGCA GACAGCACTA CTAATTATAT GACATTTTCA 
GCCAGTCCCT ACTCAGACCC GGTCCACGTG CGAAACAGCA TGGCTCAGGG GATGGAGCAG 
CCTGAAATGT TGTGGGTTAT GGGCCCTGTT TTGGCCGTGG TCCTGATTAT CATCATCGTC 
ATCGCCATCC TGCTCTTCAA GAACAAACAG GAAAGGAAGC GAGCGTCGCC CCTACCCAAA 
GATGAGCACT CGGGCGGGGT GAAGGACTCC CTGCTGGCCA ACTCCTCCGA CCCTGTGGAG 
ATGAGAAGAC TTAACTACCA GACCCCAGGT CCCAGCTCTC ATCGCTGCCC CAACACTCCA 
AGAATGAGGG AGCACCCGCC CATCCCTGTC GTCGACCTGG CTGACCACAT AGAGCGTCTG 
AAGGCCAACG ACGGCCTACG CTTTTCCCAG GAGTACGAGT CGATTGATCC AGGGCAGCAG 
TTTACCTGGG AGAACTCCAA CATGGAAGTC AACAAGCCAA AGAATCGCTA TGCAAACGTC 
ATCGCCTATG ACCACTCCAG AGTGGTTCTC ACCTCTGTTG ACGCCGTTCC AGGCAGTGAC 
TACATCAACG CCAACTACAT CGACGGCTAC AGGAAGCAGA ACGCCTACAT CGCCACGCAG 
GGGCCACTTC CAGAGACACT GAGCGACTTC TGGAGGATGG TGTGGGAGCA AAGATCCAAC 
ACCATCGTCA TGATGACCAG ACTGGAGGAG AAGTCGCGGG TGAAGTGTGA CCAGTACTGG 
CCGTCGCGAG GAACGGAGAC CTATGGGATG ATCCAGGTCA CCATGCTGGA CACCGTGGAG 
TTGGCCACTT ACAGCGTCCG TACCTTTGCC CTCTATAAGA ATGGCTCCAG TGAGAAGAGA 
GAGGTGAGAC AGTTCCAGTT CATGGCCTGG CCAGACCACG GGGTGCCAGA GTACCCCACC 
CCAATCCTGG CCTTCCTACG GCGGGTAAAG GCCTGCAATC CTCCAGACGC AGGGCCTATG 
GTGGTCCACT GCAGCGCGGG TGTGGGCAGG ACCGGATGCT TCATCGTGAT AGATGCCATG 
CTGGAGCGCA TGAAGCACGA GAAGTCCGTA GACATCTACG GCCACGTGAC GTGCATGCGC 
GCCCAGAGGA ACTACATGGT CCAGACCGAG GACCAGTACA TCTTCATTCA CGAAGCCCTG 
CTGGAGGCCG CCACGTGCGG CAACACCGAG GTGCCGGCGC GCAACCTGTA CGCCCACATC 
CAGAAACTCA CCCAGCCCCC GCCCGGCGAG ACGGTTACTG CCATGGAGCT GGAATTCAAG 
AGACTGGCCA ACTCCAAAGC CCACACATCC CGCTTCATCA GCGCCAACTT GCCCTGCAAC 
AAGTTCAAGA ACCGCCTGGT CAACATCATG CCTTTCGAGT CCACCCGCGT CTGCCTGCAG 
CCCATCCGTG GCGTCGAGGG CTCCGACTAC ATCAATGCCA GCTGCATCGA CGGCTACAGA 
CAGCAGAAGG CCTACCTGGC CACGCAGGGC CCACTGGCAG AGACCACCGA GGACTTCTGG 
AGGATGCTGT GGGAGCACAA CTCCACCATC GTCGTCATGC TCACCAAGCT ACGAGAGATG 
GGCCGGGAGA AGTGCCATCA GTACTGGCCA GCAGAGCGTT CAGCCAGGTA CCAGTACTTT 
GTGGTGGATC CAATGGCTGA GTACAACATG CCCCAGTACA TCCTCAGAGA GTTCAAAGTC 
ACAGATGCCA GGGACGGCCA GTCCAGAACA ATCCGCCAAT TCCAGTTCAC TGACTGGCCC 
GAGCAAGGAG TGCCAAAAAC CGGAGAGGGC TTCATTGATT TTATCGGACA AGTGCACAAA 
ACCAAGGAAC AGTTCGGACA GGACGGACCA ATCACTGTAC ACTGCAGTGC TGGGGTGGGC 
AGGACCGGAG TCTTCATCAC CCTCAGCATC GTGCTGGAGA GGATGAGGTA TGAAGGAGTT 
GTCGACCTCT TCCAGACAGT GAAGACACTT CGCACCCAGC GGCCTGCCAT GGTGCAGACC 
GAGGACCAGT ACCAGTTGTG CTACAGGGCA GCATTGGAGT ACCTGGGGAG CTTTGACCAC 
TATGCAACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104931439 ( XM_019263523.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019119068.1 Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X1, mRNA.

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CloneID	OLa211346
Clone ID Related Accession (Same CDS sequence)	XM_019263528.2 , XM_019263523.2
Accession Version	XM_019263523.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	receptor-type tyrosine-protein phosphatase F isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040012.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_019263523.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTGGACATC 
AGCAGCAGCA ACGGGCGCAT TAAACAGCTT CGTTCAGGTG GTACACCAAT TAGAGGAGCT 
CTGCAAATTG AGAACAGCGA GGAGTCCGAC CAAGGGAAAT ACGAGTGTGT TGCCATGAAC 
AGCGCTGGGA CCCGTTACTC CGCTCCTGCC AATCTCTACG TACGAGTGCG AAGAGTCCCA 
CCCAGATTCT CCATTCCGCC CACCAACCAA GAGGTGATGC CAGGCGGCAG CGTCAACCTG 
ACATGCGTGG CAGTGGGCTC GCCGATGCCC TACGTCAAGT GGACGATGGG CCTGGTGGAG 
CTGACCAAGG AGGAGGAGAT GCCGATGGGA CGCAACGTGC TGGAGGTCAA CAACATCCGC 
GAGTCGGCGA ACTACACCTG CGTGGCCATC TCCTCTCTGG GCATGATAGA AGCCACCGCT 
CAGGTCACCG TCAAAGCTTT GCCAAAGCCT CCCGCCTCCC TCATTGTGAC TGAGACCACA 
GCCACCAGTG TGACTCTCAC CTGGGACTCT GGAAACCCAG AACCTGTCTC CTACTACATG 
ATCCAATACA GAGCCAAGAC CTCGGACAAC GGCTTCCAGG AAGTGGAAGG TGTGGCAACT 
ACCCGTTACA GCATCGGTGG ACTTAGCCCC TACTCTGAGT ATGAGTTTCG TGTCATGGCT 
GTCAACAACA TCGGCCGGGG GCCACCGAGC GACCCCGTGG AGACACGCAC TGGTGAACAG 
GCCCCCTCCT CGCCACCTCT GCACGTCCAA GCGCGCATGC TGAGTGCCAG CACAATGCTG 
GTCCAGTGGG AACCGCCCGA GGAACCCAAC GGGCAAATCA GGGGCTACCG GGTCTACTAC 
AGCTCGGACT TGACGGCTCC GCTCAGCGCC TGGCAGAAGC ACAACACAGA CGACAGCAGC 
CTGACGACCA TCTCGGGCCT GACTCCTGAT ATCACCTACA GCCTCAGGGT GCTGGGCTTC 
ACCTCTGTAG GTGACGGGCC ACCTTCTGAC ATCCTGCAAG TCAAAACCCA ACAGGGAGTC 
CCTGCCCAGC CGTCCAGCTT TGAGGCCGAA GCTGAGCTGG ACAGTCGCAT CATGCTAACA 
TGGCTGTGGC CCGTCCAGGA CCCCATCACT AAGTATGAGC TGCAGTACTG GGAGGCCGGC 
TCAGACAACA AGTTACATGT GAGCTTCAAC CCAGCGGGCT CCTACGCTGT GGACGGTCTA 
AAGCCTGACA CCTTGTACCG TTTCTCTCTG GCAGCCCGTT CTGAGATGGG CTTAGGTGTC 
TACACCCAAC CCATCGAGGC TCGCACCGCC CAGTCCACCC CATCAGCTCC CCCCCAGGAG 
GTACACCTGG TCAGCCTGAG CTCCACCAGC CTCAAGGTGA GTTGGGTGGC ACCGCCCGCC 
GCTAGCCGCC ACGGCGCCAT TGTGCGCTAC ACAGTCAGCT ACCAGGCCAT GGCTGGAGAA 
GACACGGAGC GGCACGAGGT GACGGGCATC GGCGCAGACG CCACTAGCTT TGTGCTGGAG 
GGCCTGGAGA AGTGGACTGA GTATCAGGTG TGGGTGAGGG CACACACTGA CGTGGGCCCC 
GGCCCAGAGA GTGCCCCAGT GAGGATGAGA ACCAAAGAGG ATGTGCCGGG AGCACCCCCC 
AGGAAGCTGG AGGTTGAGGC CATAAACTCC ACAGCAATCC GGGTCACCTG GAAGCCGCCC 
CTGCAGGGGA AGCAGCACGG CCAAATCCGG GGCTATCAAG TCATCTTCTC TCGGCTTGAA 
AACGGCGAAC CACGAGGCCA GCCTAACATC ATGGACGTTG CTTTGCCAGA GGCACAGTGG 
AATATTGAGG AGTCCACCGA GCATGAGGCC ATTATTGCCG GACTGCACTC TGAAACCACC 
TACTCGGTCA CCGTGGCAGC ATACACCACC AAAGGAGACG GAGCGCGCAG CAAAGCCAAA 
GTCATCACCA CGACGGGTGC AGTGCCAGGA AAGCCCACCA TGATGATCAG CACCACTATG 
GGCAACACAG CGCTGATTCA GTGGCAGCCG CCTAAGGAAA TGGTTGGGGA GCTGATGGGC 
TACCAGCTGC AGTACAAGCG CACTGACGAG GAAAACTACA CCTCCCGCGA GCTGAGAAAG 
ATCGATGACC ATTACACCGT CACCGGGCTG CACAAAGGTG CCACCTATGT CTTCCGCCTC 
AGTGCCCGCA ACCGCGCGGG CATCGGCGAA GCATACGTGA AAGAGATCAG CACCCCTGAA 
GACGTGCCGT CGGGCTTCCC GTTCAACCTG CAGGTCACGG GACTGACTCA GTCCAGCACC 
GAGCTGACCT GGGACCCTCC GCTGGTGGCC GAAAGGAACG GCAAGATCGT GCACTATGTG 
GTCGTTTACA GGGACATCAA CAGTCAGCAG AACAGCACCA ACCGGACGAG CGAAACGCAC 
ATGACCATCC AGGGCCTTAA TCCCGACACC ACCTACGATA TCCGGGTGCA GGCGTTCACC 
AGTAAGGGCG GAGGACCCCT CAGCCCTAGC ATCCAGAGCA GGACCATGTC GAACGATTTT 
CCCACCCTCA ACTTTGGTGT CAAAGCCGTC ATGAAAACAT CTGTCCTCCT CACCTGGGAT 
TTGCCACCGA ACTACAAATC CCAAGTGCCT TTCAAGATCC TGTACAACCA GCAAAGCGTG 
GAAGTGCAGG GATCCCTGAA GAGGAGGCTG ATCACGCAGC TGCAGCCAGA CACGGACTAC 
TCTTTTGTGC TGACAAGCAG GGGCAACATT GCTGGTGGTC TACAGCAGCA GGTGTCCATC 
CGCACCGCTC CCGACCTGAT CAACGCCAAG CCCAGCACAC ATCAGCCTCA GGGTAGCAAG 
ATGACAATCA ACCTGCCCAA GGTCCAGACC ACCACGCGTG TCAGGTGGTA CTACATCGTG 
GTGGTGCCGG TGTCCCAGTC AACGCAGGGG TGGAAGAATC CAGACGAGAT GGACCTGGAC 
GAGCTGTTCA AGTCAAGTGA AGGCCCCGTC CTGAGGCGCA GACGTCACCT GGATTCCTCC 
AAACCTTATA TCGCTGCCAA GTTGGCCTCG TTGCCGACCA CCTTCACCCT GGGTGATGAG 
AAGAGATACA ACGGCTTCTA CAACAAGCCC CTGAGCAGTC CGCAGGAGTA TCTCTGCTTT 
GTCCTGGCAG TACTCCGATC CGAAGGGGCA GACAGCACTA CTAATTATAT GACATTTTCA 
GCCAGTCCCT ACTCAGACCC GGTCCACGTG CGAAACAGCA TGGCTCAGGG GATGGAGCAG 
CCTGAAATGT TGTGGGTTAT GGGCCCTGTT TTGGCCGTGG TCCTGATTAT CATCATCGTC 
ATCGCCATCC TGCTCTTCAA GAACAAACAG GAAAGGAAGC GAGCGTCGCC CCTACCCAAA 
GATGAGCACT CGGGCGGGGT GAAGGACTCC CTGCTGGCCA ACTCCTCCGA CCCTGTGGAG 
ATGAGAAGAC TTAACTACCA GACCCCAGGT CCCAGCTCTC ATCGCTGCCC CAACACTCCA 
AGAATGAGGG AGCACCCGCC CATCCCTGTC GTCGACCTGG CTGACCACAT AGAGCGTCTG 
AAGGCCAACG ACGGCCTACG CTTTTCCCAG GAGTACGAGT CGATTGATCC AGGGCAGCAG 
TTTACCTGGG AGAACTCCAA CATGGAAGTC AACAAGCCAA AGAATCGCTA TGCAAACGTC 
ATCGCCTATG ACCACTCCAG AGTGGTTCTC ACCTCTGTTG ACGCCGTTCC AGGCAGTGAC 
TACATCAACG CCAACTACAT CGACGGCTAC AGGAAGCAGA ACGCCTACAT CGCCACGCAG 
GGGCCACTTC CAGAGACACT GAGCGACTTC TGGAGGATGG TGTGGGAGCA AAGATCCAAC 
ACCATCGTCA TGATGACCAG ACTGGAGGAG AAGTCGCGGG TGAAGTGTGA CCAGTACTGG 
CCGTCGCGAG GAACGGAGAC CTATGGGATG ATCCAGGTCA CCATGCTGGA CACCGTGGAG 
TTGGCCACTT ACAGCGTCCG TACCTTTGCC CTCTATAAGA ATGGCTCCAG TGAGAAGAGA 
GAGGTGAGAC AGTTCCAGTT CATGGCCTGG CCAGACCACG GGGTGCCAGA GTACCCCACC 
CCAATCCTGG CCTTCCTACG GCGGGTAAAG GCCTGCAATC CTCCAGACGC AGGGCCTATG 
GTGGTCCACT GCAGCGCGGG TGTGGGCAGG ACCGGATGCT TCATCGTGAT AGATGCCATG 
CTGGAGCGCA TGAAGCACGA GAAGTCCGTA GACATCTACG GCCACGTGAC GTGCATGCGC 
GCCCAGAGGA ACTACATGGT CCAGACCGAG GACCAGTACA TCTTCATTCA CGAAGCCCTG 
CTGGAGGCCG CCACGTGCGG CAACACCGAG GTGCCGGCGC GCAACCTGTA CGCCCACATC 
CAGAAACTCA CCCAGCCCCC GCCCGGCGAG ACGGTTACTG CCATGGAGCT GGAATTCAAG 
AGACTGGCCA ACTCCAAAGC CCACACATCC CGCTTCATCA GCGCCAACTT GCCCTGCAAC 
AAGTTCAAGA ACCGCCTGGT CAACATCATG CCTTTCGAGT CCACCCGCGT CTGCCTGCAG 
CCCATCCGTG GCGTCGAGGG CTCCGACTAC ATCAATGCCA GCTGCATCGA CGGCTACAGA 
CAGCAGAAGG CCTACCTGGC CACGCAGGGC CCACTGGCAG AGACCACCGA GGACTTCTGG 
AGGATGCTGT GGGAGCACAA CTCCACCATC GTCGTCATGC TCACCAAGCT ACGAGAGATG 
GGCCGGGAGA AGTGCCATCA GTACTGGCCA GCAGAGCGTT CAGCCAGGTA CCAGTACTTT 
GTGGTGGATC CAATGGCTGA GTACAACATG CCCCAGTACA TCCTCAGAGA GTTCAAAGTC 
ACAGATGCCA GGGACGGCCA GTCCAGAACA ATCCGCCAAT TCCAGTTCAC TGACTGGCCC 
GAGCAAGGAG TGCCAAAAAC CGGAGAGGGC TTCATTGATT TTATCGGACA AGTGCACAAA 
ACCAAGGAAC AGTTCGGACA GGACGGACCA ATCACTGTAC ACTGCAGTGC TGGGGTGGGC 
AGGACCGGAG TCTTCATCAC CCTCAGCATC GTGCTGGAGA GGATGAGGTA TGAAGGAGTT 
GTCGACCTCT TCCAGACAGT GAAGACACTT CGCACCCAGC GGCCTGCCAT GGTGCAGACC 
GAGGACCAGT ACCAGTTGTG CTACAGGGCA GCATTGGAGT ACCTGGGGAG CTTTGACCAC 
TATGCAACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019119068.1
CDS	494..6265
Translation	MVPNIHLAVPFLPRGALLFLILLSCVVFSSLADSMPSFVKSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVEKTRTATMLCAASGNPDPEISWFKDMLPVDISSSNGRIKQLRSGGTPIRGALQIENSEESDQGKYECVAMNSAGTRYSAPANLYVRVRRVPPRFSIPPTNQEVMPGGSVNLTCVAVGSPMPYVKWTMGLVELTKEEEMPMGRNVLEVNNIRESANYTCVAISSLGMIEATAQVTVKALPKPPASLIVTETTATSVTLTWDSGNPEPVSYYMIQYRAKTSDNGFQEVEGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSDPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRVYYSSDLTAPLSAWQKHNTDDSSLTTISGLTPDITYSLRVLGFTSVGDGPPSDILQVKTQQGVPAQPSSFEAEAELDSRIMLTWLWPVQDPITKYELQYWEAGSDNKLHVSFNPAGSYAVDGLKPDTLYRFSLAARSEMGLGVYTQPIEARTAQSTPSAPPQEVHLVSLSSTSLKVSWVAPPAASRHGAIVRYTVSYQAMAGEDTERHEVTGIGADATSFVLEGLEKWTEYQVWVRAHTDVGPGPESAPVRMRTKEDVPGAPPRKLEVEAINSTAIRVTWKPPLQGKQHGQIRGYQVIFSRLENGEPRGQPNIMDVALPEAQWNIEESTEHEAIIAGLHSETTYSVTVAAYTTKGDGARSKAKVITTTGAVPGKPTMMISTTMGNTALIQWQPPKEMVGELMGYQLQYKRTDEENYTSRELRKIDDHYTVTGLHKGATYVFRLSARNRAGIGEAYVKEISTPEDVPSGFPFNLQVTGLTQSSTELTWDPPLVAERNGKIVHYVVVYRDINSQQNSTNRTSETHMTIQGLNPDTTYDIRVQAFTSKGGGPLSPSIQSRTMSNDFPTLNFGVKAVMKTSVLLTWDLPPNYKSQVPFKILYNQQSVEVQGSLKRRLITQLQPDTDYSFVLTSRGNIAGGLQQQVSIRTAPDLINAKPSTHQPQGSKMTINLPKVQTTTRVRWYYIVVVPVSQSTQGWKNPDEMDLDELFKSSEGPVLRRRRHLDSSKPYIAAKLASLPTTFTLGDEKRYNGFYNKPLSSPQEYLCFVLAVLRSEGADSTTNYMTFSASPYSDPVHVRNSMAQGMEQPEMLWVMGPVLAVVLIIIIVIAILLFKNKQERKRASPLPKDEHSGGVKDSLLANSSDPVEMRRLNYQTPGPSSHRCPNTPRMREHPPIPVVDLADHIERLKANDGLRFSQEYESIDPGQQFTWENSNMEVNKPKNRYANVIAYDHSRVVLTSVDAVPGSDYINANYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRSNTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVTMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRAQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPPPGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYLATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_019263523.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019263523.2

ORF Insert Sequence:

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTGGACATC 
AGCAGCAGCA ACGGGCGCAT TAAACAGCTT CGTTCAGGTG GTACACCAAT TAGAGGAGCT 
CTGCAAATTG AGAACAGCGA GGAGTCCGAC CAAGGGAAAT ACGAGTGTGT TGCCATGAAC 
AGCGCTGGGA CCCGTTACTC CGCTCCTGCC AATCTCTACG TACGAGTGCG AAGAGTCCCA 
CCCAGATTCT CCATTCCGCC CACCAACCAA GAGGTGATGC CAGGCGGCAG CGTCAACCTG 
ACATGCGTGG CAGTGGGCTC GCCGATGCCC TACGTCAAGT GGACGATGGG CCTGGTGGAG 
CTGACCAAGG AGGAGGAGAT GCCGATGGGA CGCAACGTGC TGGAGGTCAA CAACATCCGC 
GAGTCGGCGA ACTACACCTG CGTGGCCATC TCCTCTCTGG GCATGATAGA AGCCACCGCT 
CAGGTCACCG TCAAAGCTTT GCCAAAGCCT CCCGCCTCCC TCATTGTGAC TGAGACCACA 
GCCACCAGTG TGACTCTCAC CTGGGACTCT GGAAACCCAG AACCTGTCTC CTACTACATG 
ATCCAATACA GAGCCAAGAC CTCGGACAAC GGCTTCCAGG AAGTGGAAGG TGTGGCAACT 
ACCCGTTACA GCATCGGTGG ACTTAGCCCC TACTCTGAGT ATGAGTTTCG TGTCATGGCT 
GTCAACAACA TCGGCCGGGG GCCACCGAGC GACCCCGTGG AGACACGCAC TGGTGAACAG 
GCCCCCTCCT CGCCACCTCT GCACGTCCAA GCGCGCATGC TGAGTGCCAG CACAATGCTG 
GTCCAGTGGG AACCGCCCGA GGAACCCAAC GGGCAAATCA GGGGCTACCG GGTCTACTAC 
AGCTCGGACT TGACGGCTCC GCTCAGCGCC TGGCAGAAGC ACAACACAGA CGACAGCAGC 
CTGACGACCA TCTCGGGCCT GACTCCTGAT ATCACCTACA GCCTCAGGGT GCTGGGCTTC 
ACCTCTGTAG GTGACGGGCC ACCTTCTGAC ATCCTGCAAG TCAAAACCCA ACAGGGAGTC 
CCTGCCCAGC CGTCCAGCTT TGAGGCCGAA GCTGAGCTGG ACAGTCGCAT CATGCTAACA 
TGGCTGTGGC CCGTCCAGGA CCCCATCACT AAGTATGAGC TGCAGTACTG GGAGGCCGGC 
TCAGACAACA AGTTACATGT GAGCTTCAAC CCAGCGGGCT CCTACGCTGT GGACGGTCTA 
AAGCCTGACA CCTTGTACCG TTTCTCTCTG GCAGCCCGTT CTGAGATGGG CTTAGGTGTC 
TACACCCAAC CCATCGAGGC TCGCACCGCC CAGTCCACCC CATCAGCTCC CCCCCAGGAG 
GTACACCTGG TCAGCCTGAG CTCCACCAGC CTCAAGGTGA GTTGGGTGGC ACCGCCCGCC 
GCTAGCCGCC ACGGCGCCAT TGTGCGCTAC ACAGTCAGCT ACCAGGCCAT GGCTGGAGAA 
GACACGGAGC GGCACGAGGT GACGGGCATC GGCGCAGACG CCACTAGCTT TGTGCTGGAG 
GGCCTGGAGA AGTGGACTGA GTATCAGGTG TGGGTGAGGG CACACACTGA CGTGGGCCCC 
GGCCCAGAGA GTGCCCCAGT GAGGATGAGA ACCAAAGAGG ATGTGCCGGG AGCACCCCCC 
AGGAAGCTGG AGGTTGAGGC CATAAACTCC ACAGCAATCC GGGTCACCTG GAAGCCGCCC 
CTGCAGGGGA AGCAGCACGG CCAAATCCGG GGCTATCAAG TCATCTTCTC TCGGCTTGAA 
AACGGCGAAC CACGAGGCCA GCCTAACATC ATGGACGTTG CTTTGCCAGA GGCACAGTGG 
AATATTGAGG AGTCCACCGA GCATGAGGCC ATTATTGCCG GACTGCACTC TGAAACCACC 
TACTCGGTCA CCGTGGCAGC ATACACCACC AAAGGAGACG GAGCGCGCAG CAAAGCCAAA 
GTCATCACCA CGACGGGTGC AGTGCCAGGA AAGCCCACCA TGATGATCAG CACCACTATG 
GGCAACACAG CGCTGATTCA GTGGCAGCCG CCTAAGGAAA TGGTTGGGGA GCTGATGGGC 
TACCAGCTGC AGTACAAGCG CACTGACGAG GAAAACTACA CCTCCCGCGA GCTGAGAAAG 
ATCGATGACC ATTACACCGT CACCGGGCTG CACAAAGGTG CCACCTATGT CTTCCGCCTC 
AGTGCCCGCA ACCGCGCGGG CATCGGCGAA GCATACGTGA AAGAGATCAG CACCCCTGAA 
GACGTGCCGT CGGGCTTCCC GTTCAACCTG CAGGTCACGG GACTGACTCA GTCCAGCACC 
GAGCTGACCT GGGACCCTCC GCTGGTGGCC GAAAGGAACG GCAAGATCGT GCACTATGTG 
GTCGTTTACA GGGACATCAA CAGTCAGCAG AACAGCACCA ACCGGACGAG CGAAACGCAC 
ATGACCATCC AGGGCCTTAA TCCCGACACC ACCTACGATA TCCGGGTGCA GGCGTTCACC 
AGTAAGGGCG GAGGACCCCT CAGCCCTAGC ATCCAGAGCA GGACCATGTC GAACGATTTT 
CCCACCCTCA ACTTTGGTGT CAAAGCCGTC ATGAAAACAT CTGTCCTCCT CACCTGGGAT 
TTGCCACCGA ACTACAAATC CCAAGTGCCT TTCAAGATCC TGTACAACCA GCAAAGCGTG 
GAAGTGCAGG GATCCCTGAA GAGGAGGCTG ATCACGCAGC TGCAGCCAGA CACGGACTAC 
TCTTTTGTGC TGACAAGCAG GGGCAACATT GCTGGTGGTC TACAGCAGCA GGTGTCCATC 
CGCACCGCTC CCGACCTGAT CAACGCCAAG CCCAGCACAC ATCAGCCTCA GGGTAGCAAG 
ATGACAATCA ACCTGCCCAA GGTCCAGACC ACCACGCGTG TCAGGTGGTA CTACATCGTG 
GTGGTGCCGG TGTCCCAGTC AACGCAGGGG TGGAAGAATC CAGACGAGAT GGACCTGGAC 
GAGCTGTTCA AGTCAAGTGA AGGCCCCGTC CTGAGGCGCA GACGTCACCT GGATTCCTCC 
AAACCTTATA TCGCTGCCAA GTTGGCCTCG TTGCCGACCA CCTTCACCCT GGGTGATGAG 
AAGAGATACA ACGGCTTCTA CAACAAGCCC CTGAGCAGTC CGCAGGAGTA TCTCTGCTTT 
GTCCTGGCAG TACTCCGATC CGAAGGGGCA GACAGCACTA CTAATTATAT GACATTTTCA 
GCCAGTCCCT ACTCAGACCC GGTCCACGTG CGAAACAGCA TGGCTCAGGG GATGGAGCAG 
CCTGAAATGT TGTGGGTTAT GGGCCCTGTT TTGGCCGTGG TCCTGATTAT CATCATCGTC 
ATCGCCATCC TGCTCTTCAA GAACAAACAG GAAAGGAAGC GAGCGTCGCC CCTACCCAAA 
GATGAGCACT CGGGCGGGGT GAAGGACTCC CTGCTGGCCA ACTCCTCCGA CCCTGTGGAG 
ATGAGAAGAC TTAACTACCA GACCCCAGGT CCCAGCTCTC ATCGCTGCCC CAACACTCCA 
AGAATGAGGG AGCACCCGCC CATCCCTGTC GTCGACCTGG CTGACCACAT AGAGCGTCTG 
AAGGCCAACG ACGGCCTACG CTTTTCCCAG GAGTACGAGT CGATTGATCC AGGGCAGCAG 
TTTACCTGGG AGAACTCCAA CATGGAAGTC AACAAGCCAA AGAATCGCTA TGCAAACGTC 
ATCGCCTATG ACCACTCCAG AGTGGTTCTC ACCTCTGTTG ACGCCGTTCC AGGCAGTGAC 
TACATCAACG CCAACTACAT CGACGGCTAC AGGAAGCAGA ACGCCTACAT CGCCACGCAG 
GGGCCACTTC CAGAGACACT GAGCGACTTC TGGAGGATGG TGTGGGAGCA AAGATCCAAC 
ACCATCGTCA TGATGACCAG ACTGGAGGAG AAGTCGCGGG TGAAGTGTGA CCAGTACTGG 
CCGTCGCGAG GAACGGAGAC CTATGGGATG ATCCAGGTCA CCATGCTGGA CACCGTGGAG 
TTGGCCACTT ACAGCGTCCG TACCTTTGCC CTCTATAAGA ATGGCTCCAG TGAGAAGAGA 
GAGGTGAGAC AGTTCCAGTT CATGGCCTGG CCAGACCACG GGGTGCCAGA GTACCCCACC 
CCAATCCTGG CCTTCCTACG GCGGGTAAAG GCCTGCAATC CTCCAGACGC AGGGCCTATG 
GTGGTCCACT GCAGCGCGGG TGTGGGCAGG ACCGGATGCT TCATCGTGAT AGATGCCATG 
CTGGAGCGCA TGAAGCACGA GAAGTCCGTA GACATCTACG GCCACGTGAC GTGCATGCGC 
GCCCAGAGGA ACTACATGGT CCAGACCGAG GACCAGTACA TCTTCATTCA CGAAGCCCTG 
CTGGAGGCCG CCACGTGCGG CAACACCGAG GTGCCGGCGC GCAACCTGTA CGCCCACATC 
CAGAAACTCA CCCAGCCCCC GCCCGGCGAG ACGGTTACTG CCATGGAGCT GGAATTCAAG 
AGACTGGCCA ACTCCAAAGC CCACACATCC CGCTTCATCA GCGCCAACTT GCCCTGCAAC 
AAGTTCAAGA ACCGCCTGGT CAACATCATG CCTTTCGAGT CCACCCGCGT CTGCCTGCAG 
CCCATCCGTG GCGTCGAGGG CTCCGACTAC ATCAATGCCA GCTGCATCGA CGGCTACAGA 
CAGCAGAAGG CCTACCTGGC CACGCAGGGC CCACTGGCAG AGACCACCGA GGACTTCTGG 
AGGATGCTGT GGGAGCACAA CTCCACCATC GTCGTCATGC TCACCAAGCT ACGAGAGATG 
GGCCGGGAGA AGTGCCATCA GTACTGGCCA GCAGAGCGTT CAGCCAGGTA CCAGTACTTT 
GTGGTGGATC CAATGGCTGA GTACAACATG CCCCAGTACA TCCTCAGAGA GTTCAAAGTC 
ACAGATGCCA GGGACGGCCA GTCCAGAACA ATCCGCCAAT TCCAGTTCAC TGACTGGCCC 
GAGCAAGGAG TGCCAAAAAC CGGAGAGGGC TTCATTGATT TTATCGGACA AGTGCACAAA 
ACCAAGGAAC AGTTCGGACA GGACGGACCA ATCACTGTAC ACTGCAGTGC TGGGGTGGGC 
AGGACCGGAG TCTTCATCAC CCTCAGCATC GTGCTGGAGA GGATGAGGTA TGAAGGAGTT 
GTCGACCTCT TCCAGACAGT GAAGACACTT CGCACCCAGC GGCCTGCCAT GGTGCAGACC 
GAGGACCAGT ACCAGTTGTG CTACAGGGCA GCATTGGAGT ACCTGGGGAG CTTTGACCAC 
TATGCAACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104931439 ( XM_019263532.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019119077.1 Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X3, mRNA.

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CloneID	OLa211347
Clone ID Related Accession (Same CDS sequence)	XM_019263532.2
Accession Version	XM_019263532.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	receptor-type tyrosine-protein phosphatase F isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040012.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_019263532.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTGGACATC 
AGCAGCAGCA ACGGGCGCAT TAAACAGCTT CGTTCAGGTG GTACACCAAT TAGAGGAGCT 
CTGCAAATTG AGAACAGCGA GGAGTCCGAC CAAGGGAAAT ACGAGTGTGT TGCCATGAAC 
AGCGCTGGGA CCCGTTACTC CGCTCCTGCC AATCTCTACG TACGAGTGCG AAGAGTCCCA 
CCCAGATTCT CCATTCCGCC CACCAACCAA GAGGTGATGC CAGGCGGCAG CGTCAACCTG 
ACATGCGTGG CAGTGGGCTC GCCGATGCCC TACGTCAAGT GGACGATGGG CCTGGTGGAG 
CTGACCAAGG AGGAGGAGAT GCCGATGGGA CGCAACGTGC TGGAGGTCAA CAACATCCGC 
GAGTCGGCGA ACTACACCTG CGTGGCCATC TCCTCTCTGG GCATGATAGA AGCCACCGCT 
CAGGTCACCG TCAAAGCTTT GCCAAAGCCT CCCGCCTCCC TCATTGTGAC TGAGACCACA 
GCCACCAGTG TGACTCTCAC CTGGGACTCT GGAAACCCAG AACCTGTCTC CTACTACATG 
ATCCAATACA GAGCCAAGAC CTCGGACAAC GGCTTCCAGG AAGTGGAAGG TGTGGCAACT 
ACCCGTTACA GCATCGGTGG ACTTAGCCCC TACTCTGAGT ATGAGTTTCG TGTCATGGCT 
GTCAACAACA TCGGCCGGGG GCCACCGAGC GACCCCGTGG AGACACGCAC TGGTGAACAG 
GCCCCCTCCT CGCCACCTCT GCACGTCCAA GCGCGCATGC TGAGTGCCAG CACAATGCTG 
GTCCAGTGGG AACCGCCCGA GGAACCCAAC GGGCAAATCA GGGGCTACCG GGTCTACTAC 
AGCTCGGACT TGACGGCTCC GCTCAGCGCC TGGCAGAAGC ACAACACAGA CGACAGCAGC 
CTGACGACCA TCTCGGGCCT GACTCCTGAT ATCACCTACA GCCTCAGGGT GCTGGGCTTC 
ACCTCTGTAG GTGACGGGCC ACCTTCTGAC ATCCTGCAAG TCAAAACCCA ACAGGGAGTC 
CCTGCCCAGC CGTCCAGCTT TGAGGCCGAA GCTGAGCTGG ACAGTCGCAT CATGCTAACA 
TGGCTGTGGC CCGTCCAGGA CCCCATCACT AAGTATGAGC TGCAGTACTG GGAGGCCGGC 
TCAGACAACA AGTTACATGT GAGCTTCAAC CCAGCGGGCT CCTACGCTGT GGACGGTCTA 
AAGCCTGACA CCTTGTACCG TTTCTCTCTG GCAGCCCGTT CTGAGATGGG CTTAGGTGTC 
TACACCCAAC CCATCGAGGC TCGCACCGCC CAGTCCACCC CATCAGCTCC CCCCCAGGAG 
GTACACCTGG TCAGCCTGAG CTCCACCAGC CTCAAGGTGA GTTGGGTGGC ACCGCCCGCC 
GCTAGCCGCC ACGGCGCCAT TGTGCGCTAC ACAGTCAGCT ACCAGGCCAT GGCTGGAGAA 
GACACGGAGC GGCACGAGGT GACGGGCATC GGCGCAGACG CCACTAGCTT TGTGCTGGAG 
GGCCTGGAGA AGTGGACTGA GTATCAGGTG TGGGTGAGGG CACACACTGA CGTGGGCCCC 
GGCCCAGAGA GTGCCCCAGT GAGGATGAGA ACCAAAGAGG ATGTGCCGGG AGCACCCCCC 
AGGAAGCTGG AGGTTGAGGC CATAAACTCC ACAGCAATCC GGGTCACCTG GAAGCCGCCC 
CTGCAGGGGA AGCAGCACGG CCAAATCCGG GGCTATCAAG TCATCTTCTC TCGGCTTGAA 
AACGGCGAAC CACGAGGCCA GCCTAACATC ATGGACGTTG CTTTGCCAGA GGCACAGTGG 
AATATTGAGG AGTCCACCGA GCATGAGGCC ATTATTGCCG GACTGCACTC TGAAACCACC 
TACTCGGTCA CCGTGGCAGC ATACACCACC AAAGGAGACG GAGCGCGCAG CAAAGCCAAA 
GTCATCACCA CGACGGGTGC AGTGCCAGGA AAGCCCACCA TGATGATCAG CACCACTATG 
GGCAACACAG CGCTGATTCA GTGGCAGCCG CCTAAGGAAA TGGTTGGGGA GCTGATGGGC 
TACCAGCTGC AGTACAAGCG CACTGACGAG GAAAACTACA CCTCCCGCGA GCTGAGAAAG 
ATCGATGACC ATTACACCGT CACCGGGCTG CACAAAGGTG CCACCTATGT CTTCCGCCTC 
AGTGCCCGCA ACCGCGCGGG CATCGGCGAA GCATACGTGA AAGAGATCAG CACCCCTGAA 
GACGTGCCGT CGGGCTTCCC GTTCAACCTG CAGGTCACGG GACTGACTCA GTCCAGCACC 
GAGCTGACCT GGGACCCTCC GCTGGTGGCC GAAAGGAACG GCAAGATCGT GCACTATGTG 
GTCGTTTACA GGGACATCAA CAGTCAGCAG AACAGCACCA ACCGGACGAG CGAAACGCAC 
ATGACCATCC AGGGCCTTAA TCCCGACACC ACCTACGATA TCCGGGTGCA GGCGTTCACC 
AGTAAGGGCG GAGGACCCCT CAGCCCTAGC ATCCAGAGCA GGACCATGTC GAACGATTTT 
CCCACCCTCA ACTTTGGTGT CAAAGCCGTC ATGAAAACAT CTGTCCTCCT CACCTGGGAT 
TTGCCACCGA ACTACAAATC CCAAGTGCCT TTCAAGATCC TGTACAACCA GCAAAGCGTG 
GAAGTGCAGG GATCCCTGAA GAGGAGGCTG ATCACGCAGC TGCAGCCAGA CACGGACTAC 
TCTTTTGTGC TGACAAGCAG GGGCAACATT GCTGGTGGTC TACAGCAGCA GGTGTCCATC 
CGCACCGCTC CCGACCTGAT CAACGCCAAG CCCAGCACAC ATCAGCCTCA GGGTAGCAAG 
ATGACAATCA ACCTGCCCAA GGTCCAGACC ACCACGCGTG TCAGGTGGTA CTACATCGTG 
GTGGTGCCGG TGTCCCAGTC AACGCAGGGG TGGAAGAATC CAGACGAGAT GGACCTGGAC 
GAGCTGTTCA AGTCAAGTGA AGGCCCCGTC CTGAGGCGCA GACGTCACCT GGATTCCTCC 
AAACCTTATA TCGCTGCCAA GTTGGCCTCG TTGCCGACCA CCTTCACCCT GGGTGATGAG 
AAGAGATACA ACGGCTTCTA CAACAAGCCC CTGAGCAGTC CGCAGGAGTA TCTCTGCTTT 
GTCCTGGCAG TACTCCGATC CGAAGGGGCA GACAGCACTA CTAATTATAT GACATTTTCA 
GCCAGTCCCT ACTCAGACCC GGTCCACGTG CGAAACAGCA TGGCTCAGGG GATGGAGCAG 
CCTGAAATGT TGTGGGTTAT GGGCCCTGTT TTGGCCGTGG TCCTGATTAT CATCATCGTC 
ATCGCCATCC TGCTCTTCAA GAAGAAGCGA GCGTCGCCCC TACCCAAAGA TGAGCACTCG 
GGCGGGGTGA AGGACTCCCT GCTGGCCAAC TCCTCCGACC CTGTGGAGAT GAGAAGACTT 
AACTACCAGA CCCCAGGTCC CAGCTCTCAT CGCTGCCCCA ACACTCCAAG AATGAGGGAG 
CACCCGCCCA TCCCTGTCGT CGACCTGGCT GACCACATAG AGCGTCTGAA GGCCAACGAC 
GGCCTACGCT TTTCCCAGGA GTACGAGTCG ATTGATCCAG GGCAGCAGTT TACCTGGGAG 
AACTCCAACA TGGAAGTCAA CAAGCCAAAG AATCGCTATG CAAACGTCAT CGCCTATGAC 
CACTCCAGAG TGGTTCTCAC CTCTGTTGAC GCCGTTCCAG GCAGTGACTA CATCAACGCC 
AACTACATCG ACGGCTACAG GAAGCAGAAC GCCTACATCG CCACGCAGGG GCCACTTCCA 
GAGACACTGA GCGACTTCTG GAGGATGGTG TGGGAGCAAA GATCCAACAC CATCGTCATG 
ATGACCAGAC TGGAGGAGAA GTCGCGGGTG AAGTGTGACC AGTACTGGCC GTCGCGAGGA 
ACGGAGACCT ATGGGATGAT CCAGGTCACC ATGCTGGACA CCGTGGAGTT GGCCACTTAC 
AGCGTCCGTA CCTTTGCCCT CTATAAGAAT GGCTCCAGTG AGAAGAGAGA GGTGAGACAG 
TTCCAGTTCA TGGCCTGGCC AGACCACGGG GTGCCAGAGT ACCCCACCCC AATCCTGGCC 
TTCCTACGGC GGGTAAAGGC CTGCAATCCT CCAGACGCAG GGCCTATGGT GGTCCACTGC 
AGCGCGGGTG TGGGCAGGAC CGGATGCTTC ATCGTGATAG ATGCCATGCT GGAGCGCATG 
AAGCACGAGA AGTCCGTAGA CATCTACGGC CACGTGACGT GCATGCGCGC CCAGAGGAAC 
TACATGGTCC AGACCGAGGA CCAGTACATC TTCATTCACG AAGCCCTGCT GGAGGCCGCC 
ACGTGCGGCA ACACCGAGGT GCCGGCGCGC AACCTGTACG CCCACATCCA GAAACTCACC 
CAGCCCCCGC CCGGCGAGAC GGTTACTGCC ATGGAGCTGG AATTCAAGAG ACTGGCCAAC 
TCCAAAGCCC ACACATCCCG CTTCATCAGC GCCAACTTGC CCTGCAACAA GTTCAAGAAC 
CGCCTGGTCA ACATCATGCC TTTCGAGTCC ACCCGCGTCT GCCTGCAGCC CATCCGTGGC 
GTCGAGGGCT CCGACTACAT CAATGCCAGC TGCATCGACG GCTACAGACA GCAGAAGGCC 
TACCTGGCCA CGCAGGGCCC ACTGGCAGAG ACCACCGAGG ACTTCTGGAG GATGCTGTGG 
GAGCACAACT CCACCATCGT CGTCATGCTC ACCAAGCTAC GAGAGATGGG CCGGGAGAAG 
TGCCATCAGT ACTGGCCAGC AGAGCGTTCA GCCAGGTACC AGTACTTTGT GGTGGATCCA 
ATGGCTGAGT ACAACATGCC CCAGTACATC CTCAGAGAGT TCAAAGTCAC AGATGCCAGG 
GACGGCCAGT CCAGAACAAT CCGCCAATTC CAGTTCACTG ACTGGCCCGA GCAAGGAGTG 
CCAAAAACCG GAGAGGGCTT CATTGATTTT ATCGGACAAG TGCACAAAAC CAAGGAACAG 
TTCGGACAGG ACGGACCAAT CACTGTACAC TGCAGTGCTG GGGTGGGCAG GACCGGAGTC 
TTCATCACCC TCAGCATCGT GCTGGAGAGG ATGAGGTATG AAGGAGTTGT CGACCTCTTC 
CAGACAGTGA AGACACTTCG CACCCAGCGG CCTGCCATGG TGCAGACCGA GGACCAGTAC 
CAGTTGTGCT ACAGGGCAGC ATTGGAGTAC CTGGGGAGCT TTGACCACTA TGCAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019119077.1
CDS	493..6252
Translation	MVPNIHLAVPFLPRGALLFLILLSCVVFSSLADSMPSFVKSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVEKTRTATMLCAASGNPDPEISWFKDMLPVDISSSNGRIKQLRSGGTPIRGALQIENSEESDQGKYECVAMNSAGTRYSAPANLYVRVRRVPPRFSIPPTNQEVMPGGSVNLTCVAVGSPMPYVKWTMGLVELTKEEEMPMGRNVLEVNNIRESANYTCVAISSLGMIEATAQVTVKALPKPPASLIVTETTATSVTLTWDSGNPEPVSYYMIQYRAKTSDNGFQEVEGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSDPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRVYYSSDLTAPLSAWQKHNTDDSSLTTISGLTPDITYSLRVLGFTSVGDGPPSDILQVKTQQGVPAQPSSFEAEAELDSRIMLTWLWPVQDPITKYELQYWEAGSDNKLHVSFNPAGSYAVDGLKPDTLYRFSLAARSEMGLGVYTQPIEARTAQSTPSAPPQEVHLVSLSSTSLKVSWVAPPAASRHGAIVRYTVSYQAMAGEDTERHEVTGIGADATSFVLEGLEKWTEYQVWVRAHTDVGPGPESAPVRMRTKEDVPGAPPRKLEVEAINSTAIRVTWKPPLQGKQHGQIRGYQVIFSRLENGEPRGQPNIMDVALPEAQWNIEESTEHEAIIAGLHSETTYSVTVAAYTTKGDGARSKAKVITTTGAVPGKPTMMISTTMGNTALIQWQPPKEMVGELMGYQLQYKRTDEENYTSRELRKIDDHYTVTGLHKGATYVFRLSARNRAGIGEAYVKEISTPEDVPSGFPFNLQVTGLTQSSTELTWDPPLVAERNGKIVHYVVVYRDINSQQNSTNRTSETHMTIQGLNPDTTYDIRVQAFTSKGGGPLSPSIQSRTMSNDFPTLNFGVKAVMKTSVLLTWDLPPNYKSQVPFKILYNQQSVEVQGSLKRRLITQLQPDTDYSFVLTSRGNIAGGLQQQVSIRTAPDLINAKPSTHQPQGSKMTINLPKVQTTTRVRWYYIVVVPVSQSTQGWKNPDEMDLDELFKSSEGPVLRRRRHLDSSKPYIAAKLASLPTTFTLGDEKRYNGFYNKPLSSPQEYLCFVLAVLRSEGADSTTNYMTFSASPYSDPVHVRNSMAQGMEQPEMLWVMGPVLAVVLIIIIVIAILLFKKKRASPLPKDEHSGGVKDSLLANSSDPVEMRRLNYQTPGPSSHRCPNTPRMREHPPIPVVDLADHIERLKANDGLRFSQEYESIDPGQQFTWENSNMEVNKPKNRYANVIAYDHSRVVLTSVDAVPGSDYINANYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRSNTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVTMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRAQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPPPGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYLATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_019263532.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019263532.2

ORF Insert Sequence:

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTGGACATC 
AGCAGCAGCA ACGGGCGCAT TAAACAGCTT CGTTCAGGTG GTACACCAAT TAGAGGAGCT 
CTGCAAATTG AGAACAGCGA GGAGTCCGAC CAAGGGAAAT ACGAGTGTGT TGCCATGAAC 
AGCGCTGGGA CCCGTTACTC CGCTCCTGCC AATCTCTACG TACGAGTGCG AAGAGTCCCA 
CCCAGATTCT CCATTCCGCC CACCAACCAA GAGGTGATGC CAGGCGGCAG CGTCAACCTG 
ACATGCGTGG CAGTGGGCTC GCCGATGCCC TACGTCAAGT GGACGATGGG CCTGGTGGAG 
CTGACCAAGG AGGAGGAGAT GCCGATGGGA CGCAACGTGC TGGAGGTCAA CAACATCCGC 
GAGTCGGCGA ACTACACCTG CGTGGCCATC TCCTCTCTGG GCATGATAGA AGCCACCGCT 
CAGGTCACCG TCAAAGCTTT GCCAAAGCCT CCCGCCTCCC TCATTGTGAC TGAGACCACA 
GCCACCAGTG TGACTCTCAC CTGGGACTCT GGAAACCCAG AACCTGTCTC CTACTACATG 
ATCCAATACA GAGCCAAGAC CTCGGACAAC GGCTTCCAGG AAGTGGAAGG TGTGGCAACT 
ACCCGTTACA GCATCGGTGG ACTTAGCCCC TACTCTGAGT ATGAGTTTCG TGTCATGGCT 
GTCAACAACA TCGGCCGGGG GCCACCGAGC GACCCCGTGG AGACACGCAC TGGTGAACAG 
GCCCCCTCCT CGCCACCTCT GCACGTCCAA GCGCGCATGC TGAGTGCCAG CACAATGCTG 
GTCCAGTGGG AACCGCCCGA GGAACCCAAC GGGCAAATCA GGGGCTACCG GGTCTACTAC 
AGCTCGGACT TGACGGCTCC GCTCAGCGCC TGGCAGAAGC ACAACACAGA CGACAGCAGC 
CTGACGACCA TCTCGGGCCT GACTCCTGAT ATCACCTACA GCCTCAGGGT GCTGGGCTTC 
ACCTCTGTAG GTGACGGGCC ACCTTCTGAC ATCCTGCAAG TCAAAACCCA ACAGGGAGTC 
CCTGCCCAGC CGTCCAGCTT TGAGGCCGAA GCTGAGCTGG ACAGTCGCAT CATGCTAACA 
TGGCTGTGGC CCGTCCAGGA CCCCATCACT AAGTATGAGC TGCAGTACTG GGAGGCCGGC 
TCAGACAACA AGTTACATGT GAGCTTCAAC CCAGCGGGCT CCTACGCTGT GGACGGTCTA 
AAGCCTGACA CCTTGTACCG TTTCTCTCTG GCAGCCCGTT CTGAGATGGG CTTAGGTGTC 
TACACCCAAC CCATCGAGGC TCGCACCGCC CAGTCCACCC CATCAGCTCC CCCCCAGGAG 
GTACACCTGG TCAGCCTGAG CTCCACCAGC CTCAAGGTGA GTTGGGTGGC ACCGCCCGCC 
GCTAGCCGCC ACGGCGCCAT TGTGCGCTAC ACAGTCAGCT ACCAGGCCAT GGCTGGAGAA 
GACACGGAGC GGCACGAGGT GACGGGCATC GGCGCAGACG CCACTAGCTT TGTGCTGGAG 
GGCCTGGAGA AGTGGACTGA GTATCAGGTG TGGGTGAGGG CACACACTGA CGTGGGCCCC 
GGCCCAGAGA GTGCCCCAGT GAGGATGAGA ACCAAAGAGG ATGTGCCGGG AGCACCCCCC 
AGGAAGCTGG AGGTTGAGGC CATAAACTCC ACAGCAATCC GGGTCACCTG GAAGCCGCCC 
CTGCAGGGGA AGCAGCACGG CCAAATCCGG GGCTATCAAG TCATCTTCTC TCGGCTTGAA 
AACGGCGAAC CACGAGGCCA GCCTAACATC ATGGACGTTG CTTTGCCAGA GGCACAGTGG 
AATATTGAGG AGTCCACCGA GCATGAGGCC ATTATTGCCG GACTGCACTC TGAAACCACC 
TACTCGGTCA CCGTGGCAGC ATACACCACC AAAGGAGACG GAGCGCGCAG CAAAGCCAAA 
GTCATCACCA CGACGGGTGC AGTGCCAGGA AAGCCCACCA TGATGATCAG CACCACTATG 
GGCAACACAG CGCTGATTCA GTGGCAGCCG CCTAAGGAAA TGGTTGGGGA GCTGATGGGC 
TACCAGCTGC AGTACAAGCG CACTGACGAG GAAAACTACA CCTCCCGCGA GCTGAGAAAG 
ATCGATGACC ATTACACCGT CACCGGGCTG CACAAAGGTG CCACCTATGT CTTCCGCCTC 
AGTGCCCGCA ACCGCGCGGG CATCGGCGAA GCATACGTGA AAGAGATCAG CACCCCTGAA 
GACGTGCCGT CGGGCTTCCC GTTCAACCTG CAGGTCACGG GACTGACTCA GTCCAGCACC 
GAGCTGACCT GGGACCCTCC GCTGGTGGCC GAAAGGAACG GCAAGATCGT GCACTATGTG 
GTCGTTTACA GGGACATCAA CAGTCAGCAG AACAGCACCA ACCGGACGAG CGAAACGCAC 
ATGACCATCC AGGGCCTTAA TCCCGACACC ACCTACGATA TCCGGGTGCA GGCGTTCACC 
AGTAAGGGCG GAGGACCCCT CAGCCCTAGC ATCCAGAGCA GGACCATGTC GAACGATTTT 
CCCACCCTCA ACTTTGGTGT CAAAGCCGTC ATGAAAACAT CTGTCCTCCT CACCTGGGAT 
TTGCCACCGA ACTACAAATC CCAAGTGCCT TTCAAGATCC TGTACAACCA GCAAAGCGTG 
GAAGTGCAGG GATCCCTGAA GAGGAGGCTG ATCACGCAGC TGCAGCCAGA CACGGACTAC 
TCTTTTGTGC TGACAAGCAG GGGCAACATT GCTGGTGGTC TACAGCAGCA GGTGTCCATC 
CGCACCGCTC CCGACCTGAT CAACGCCAAG CCCAGCACAC ATCAGCCTCA GGGTAGCAAG 
ATGACAATCA ACCTGCCCAA GGTCCAGACC ACCACGCGTG TCAGGTGGTA CTACATCGTG 
GTGGTGCCGG TGTCCCAGTC AACGCAGGGG TGGAAGAATC CAGACGAGAT GGACCTGGAC 
GAGCTGTTCA AGTCAAGTGA AGGCCCCGTC CTGAGGCGCA GACGTCACCT GGATTCCTCC 
AAACCTTATA TCGCTGCCAA GTTGGCCTCG TTGCCGACCA CCTTCACCCT GGGTGATGAG 
AAGAGATACA ACGGCTTCTA CAACAAGCCC CTGAGCAGTC CGCAGGAGTA TCTCTGCTTT 
GTCCTGGCAG TACTCCGATC CGAAGGGGCA GACAGCACTA CTAATTATAT GACATTTTCA 
GCCAGTCCCT ACTCAGACCC GGTCCACGTG CGAAACAGCA TGGCTCAGGG GATGGAGCAG 
CCTGAAATGT TGTGGGTTAT GGGCCCTGTT TTGGCCGTGG TCCTGATTAT CATCATCGTC 
ATCGCCATCC TGCTCTTCAA GAAGAAGCGA GCGTCGCCCC TACCCAAAGA TGAGCACTCG 
GGCGGGGTGA AGGACTCCCT GCTGGCCAAC TCCTCCGACC CTGTGGAGAT GAGAAGACTT 
AACTACCAGA CCCCAGGTCC CAGCTCTCAT CGCTGCCCCA ACACTCCAAG AATGAGGGAG 
CACCCGCCCA TCCCTGTCGT CGACCTGGCT GACCACATAG AGCGTCTGAA GGCCAACGAC 
GGCCTACGCT TTTCCCAGGA GTACGAGTCG ATTGATCCAG GGCAGCAGTT TACCTGGGAG 
AACTCCAACA TGGAAGTCAA CAAGCCAAAG AATCGCTATG CAAACGTCAT CGCCTATGAC 
CACTCCAGAG TGGTTCTCAC CTCTGTTGAC GCCGTTCCAG GCAGTGACTA CATCAACGCC 
AACTACATCG ACGGCTACAG GAAGCAGAAC GCCTACATCG CCACGCAGGG GCCACTTCCA 
GAGACACTGA GCGACTTCTG GAGGATGGTG TGGGAGCAAA GATCCAACAC CATCGTCATG 
ATGACCAGAC TGGAGGAGAA GTCGCGGGTG AAGTGTGACC AGTACTGGCC GTCGCGAGGA 
ACGGAGACCT ATGGGATGAT CCAGGTCACC ATGCTGGACA CCGTGGAGTT GGCCACTTAC 
AGCGTCCGTA CCTTTGCCCT CTATAAGAAT GGCTCCAGTG AGAAGAGAGA GGTGAGACAG 
TTCCAGTTCA TGGCCTGGCC AGACCACGGG GTGCCAGAGT ACCCCACCCC AATCCTGGCC 
TTCCTACGGC GGGTAAAGGC CTGCAATCCT CCAGACGCAG GGCCTATGGT GGTCCACTGC 
AGCGCGGGTG TGGGCAGGAC CGGATGCTTC ATCGTGATAG ATGCCATGCT GGAGCGCATG 
AAGCACGAGA AGTCCGTAGA CATCTACGGC CACGTGACGT GCATGCGCGC CCAGAGGAAC 
TACATGGTCC AGACCGAGGA CCAGTACATC TTCATTCACG AAGCCCTGCT GGAGGCCGCC 
ACGTGCGGCA ACACCGAGGT GCCGGCGCGC AACCTGTACG CCCACATCCA GAAACTCACC 
CAGCCCCCGC CCGGCGAGAC GGTTACTGCC ATGGAGCTGG AATTCAAGAG ACTGGCCAAC 
TCCAAAGCCC ACACATCCCG CTTCATCAGC GCCAACTTGC CCTGCAACAA GTTCAAGAAC 
CGCCTGGTCA ACATCATGCC TTTCGAGTCC ACCCGCGTCT GCCTGCAGCC CATCCGTGGC 
GTCGAGGGCT CCGACTACAT CAATGCCAGC TGCATCGACG GCTACAGACA GCAGAAGGCC 
TACCTGGCCA CGCAGGGCCC ACTGGCAGAG ACCACCGAGG ACTTCTGGAG GATGCTGTGG 
GAGCACAACT CCACCATCGT CGTCATGCTC ACCAAGCTAC GAGAGATGGG CCGGGAGAAG 
TGCCATCAGT ACTGGCCAGC AGAGCGTTCA GCCAGGTACC AGTACTTTGT GGTGGATCCA 
ATGGCTGAGT ACAACATGCC CCAGTACATC CTCAGAGAGT TCAAAGTCAC AGATGCCAGG 
GACGGCCAGT CCAGAACAAT CCGCCAATTC CAGTTCACTG ACTGGCCCGA GCAAGGAGTG 
CCAAAAACCG GAGAGGGCTT CATTGATTTT ATCGGACAAG TGCACAAAAC CAAGGAACAG 
TTCGGACAGG ACGGACCAAT CACTGTACAC TGCAGTGCTG GGGTGGGCAG GACCGGAGTC 
TTCATCACCC TCAGCATCGT GCTGGAGAGG ATGAGGTATG AAGGAGTTGT CGACCTCTTC 
CAGACAGTGA AGACACTTCG CACCCAGCGG CCTGCCATGG TGCAGACCGA GGACCAGTAC 
CAGTTGTGCT ACAGGGCAGC ATTGGAGTAC CTGGGGAGCT TTGACCACTA TGCAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104931439 ( XM_010746444.3 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010744746.2 Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X4, mRNA.

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CloneID	OLa211348
Clone ID Related Accession (Same CDS sequence)	XM_010746444.3
Accession Version	XM_010746444.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	receptor-type tyrosine-protein phosphatase F isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040012.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_010746444.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTGGACATC 
AGCAGCAGCA ACGGGCGCAT TAAACAGCTT CGTTCAGGAG CTCTGCAAAT TGAGAACAGC 
GAGGAGTCCG ACCAAGGGAA ATACGAGTGT GTTGCCATGA ACAGCGCTGG GACCCGTTAC 
TCCGCTCCTG CCAATCTCTA CGTACGAGTG CGAAGAGTCC CACCCAGATT CTCCATTCCG 
CCCACCAACC AAGAGGTGAT GCCAGGCGGC AGCGTCAACC TGACATGCGT GGCAGTGGGC 
TCGCCGATGC CCTACGTCAA GTGGACGATG GGCCTGGTGG AGCTGACCAA GGAGGAGGAG 
ATGCCGATGG GACGCAACGT GCTGGAGGTC AACAACATCC GCGAGTCGGC GAACTACACC 
TGCGTGGCCA TCTCCTCTCT GGGCATGATA GAAGCCACCG CTCAGGTCAC CGTCAAAGCT 
TTGCCAAAGC CTCCCGCCTC CCTCATTGTG ACTGAGACCA CAGCCACCAG TGTGACTCTC 
ACCTGGGACT CTGGAAACCC AGAACCTGTC TCCTACTACA TGATCCAATA CAGAGCCAAG 
ACCTCGGACA ACGGCTTCCA GGAAGTGGAA GGTGTGGCAA CTACCCGTTA CAGCATCGGT 
GGACTTAGCC CCTACTCTGA GTATGAGTTT CGTGTCATGG CTGTCAACAA CATCGGCCGG 
GGGCCACCGA GCGACCCCGT GGAGACACGC ACTGGTGAAC AGGCCCCCTC CTCGCCACCT 
CTGCACGTCC AAGCGCGCAT GCTGAGTGCC AGCACAATGC TGGTCCAGTG GGAACCGCCC 
GAGGAACCCA ACGGGCAAAT CAGGGGCTAC CGGGTCTACT ACAGCTCGGA CTTGACGGCT 
CCGCTCAGCG CCTGGCAGAA GCACAACACA GACGACAGCA GCCTGACGAC CATCTCGGGC 
CTGACTCCTG ATATCACCTA CAGCCTCAGG GTGCTGGGCT TCACCTCTGT AGGTGACGGG 
CCACCTTCTG ACATCCTGCA AGTCAAAACC CAACAGGGAG TCCCTGCCCA GCCGTCCAGC 
TTTGAGGCCG AAGCTGAGCT GGACAGTCGC ATCATGCTAA CATGGCTGTG GCCCGTCCAG 
GACCCCATCA CTAAGTATGA GCTGCAGTAC TGGGAGGCCG GCTCAGACAA CAAGTTACAT 
GTGAGCTTCA ACCCAGCGGG CTCCTACGCT GTGGACGGTC TAAAGCCTGA CACCTTGTAC 
CGTTTCTCTC TGGCAGCCCG TTCTGAGATG GGCTTAGGTG TCTACACCCA ACCCATCGAG 
GCTCGCACCG CCCAGTCCAC CCCATCAGCT CCCCCCCAGG AGGTACACCT GGTCAGCCTG 
AGCTCCACCA GCCTCAAGGT GAGTTGGGTG GCACCGCCCG CCGCTAGCCG CCACGGCGCC 
ATTGTGCGCT ACACAGTCAG CTACCAGGCC ATGGCTGGAG AAGACACGGA GCGGCACGAG 
GTGACGGGCA TCGGCGCAGA CGCCACTAGC TTTGTGCTGG AGGGCCTGGA GAAGTGGACT 
GAGTATCAGG TGTGGGTGAG GGCACACACT GACGTGGGCC CCGGCCCAGA GAGTGCCCCA 
GTGAGGATGA GAACCAAAGA GGATGTGCCG GGAGCACCCC CCAGGAAGCT GGAGGTTGAG 
GCCATAAACT CCACAGCAAT CCGGGTCACC TGGAAGCCGC CCCTGCAGGG GAAGCAGCAC 
GGCCAAATCC GGGGCTATCA AGTCATCTTC TCTCGGCTTG AAAACGGCGA ACCACGAGGC 
CAGCCTAACA TCATGGACGT TGCTTTGCCA GAGGCACAGT GGAATATTGA GGAGTCCACC 
GAGCATGAGG CCATTATTGC CGGACTGCAC TCTGAAACCA CCTACTCGGT CACCGTGGCA 
GCATACACCA CCAAAGGAGA CGGAGCGCGC AGCAAAGCCA AAGTCATCAC CACGACGGGT 
GCAGTGCCAG GAAAGCCCAC CATGATGATC AGCACCACTA TGGGCAACAC AGCGCTGATT 
CAGTGGCAGC CGCCTAAGGA AATGGTTGGG GAGCTGATGG GCTACCAGCT GCAGTACAAG 
CGCACTGACG AGGAAAACTA CACCTCCCGC GAGCTGAGAA AGATCGATGA CCATTACACC 
GTCACCGGGC TGCACAAAGG TGCCACCTAT GTCTTCCGCC TCAGTGCCCG CAACCGCGCG 
GGCATCGGCG AAGCATACGT GAAAGAGATC AGCACCCCTG AAGACGTGCC GTCGGGCTTC 
CCGTTCAACC TGCAGGTCAC GGGACTGACT CAGTCCAGCA CCGAGCTGAC CTGGGACCCT 
CCGCTGGTGG CCGAAAGGAA CGGCAAGATC GTGCACTATG TGGTCGTTTA CAGGGACATC 
AACAGTCAGC AGAACAGCAC CAACCGGACG AGCGAAACGC ACATGACCAT CCAGGGCCTT 
AATCCCGACA CCACCTACGA TATCCGGGTG CAGGCGTTCA CCAGTAAGGG CGGAGGACCC 
CTCAGCCCTA GCATCCAGAG CAGGACCATG TCGAACGATT TTCCCACCCT CAACTTTGGT 
GTCAAAGCCG TCATGAAAAC ATCTGTCCTC CTCACCTGGG ATTTGCCACC GAACTACAAA 
TCCCAAGTGC CTTTCAAGAT CCTGTACAAC CAGCAAAGCG TGGAAGTGCA GGGATCCCTG 
AAGAGGAGGC TGATCACGCA GCTGCAGCCA GACACGGACT ACTCTTTTGT GCTGACAAGC 
AGGGGCAACA TTGCTGGTGG TCTACAGCAG CAGGTGTCCA TCCGCACCGC TCCCGACCTG 
ATCAACGCCA AGCCCAGCAC ACATCAGCCT CAGGGTAGCA AGATGACAAT CAACCTGCCC 
AAGGTCCAGA CCACCACGCG TGTCAGGTGG TACTACATCG TGGTGGTGCC GGTGTCCCAG 
TCAACGCAGG GGTGGAAGAA TCCAGACGAG ATGGACCTGG ACGAGCTGTT CAAGTCAAGT 
GAAGGCCCCG TCCTGAGGCG CAGACGTCAC CTGGATTCCT CCAAACCTTA TATCGCTGCC 
AAGTTGGCCT CGTTGCCGAC CACCTTCACC CTGGGTGATG AGAAGAGATA CAACGGCTTC 
TACAACAAGC CCCTGAGCAG TCCGCAGGAG TATCTCTGCT TTGTCCTGGC AGTACTCCGA 
TCCGAAGGGG CAGACAGCAC TACTAATTAT ATGACATTTT CAGCCAGTCC CTACTCAGAC 
CCGGTCCACG TGCGAAACAG CATGGCTCAG GGGATGGAGC AGCCTGAAAT GTTGTGGGTT 
ATGGGCCCTG TTTTGGCCGT GGTCCTGATT ATCATCATCG TCATCGCCAT CCTGCTCTTC 
AAGAACAAAC AGGAAAGGAA GCGAGCGTCG CCCCTACCCA AAGATGAGCA CTCGGGCGGG 
GTGAAGGACT CCCTGCTGGC CAACTCCTCC GACCCTGTGG AGATGAGAAG ACTTAACTAC 
CAGACCCCAG GTCCCAGCTC TCATCGCTGC CCCAACACTC CAAGAATGAG GGAGCACCCG 
CCCATCCCTG TCGTCGACCT GGCTGACCAC ATAGAGCGTC TGAAGGCCAA CGACGGCCTA 
CGCTTTTCCC AGGAGTACGA GTCGATTGAT CCAGGGCAGC AGTTTACCTG GGAGAACTCC 
AACATGGAAG TCAACAAGCC AAAGAATCGC TATGCAAACG TCATCGCCTA TGACCACTCC 
AGAGTGGTTC TCACCTCTGT TGACGCCGTT CCAGGCAGTG ACTACATCAA CGCCAACTAC 
ATCGACGGCT ACAGGAAGCA GAACGCCTAC ATCGCCACGC AGGGGCCACT TCCAGAGACA 
CTGAGCGACT TCTGGAGGAT GGTGTGGGAG CAAAGATCCA ACACCATCGT CATGATGACC 
AGACTGGAGG AGAAGTCGCG GGTGAAGTGT GACCAGTACT GGCCGTCGCG AGGAACGGAG 
ACCTATGGGA TGATCCAGGT CACCATGCTG GACACCGTGG AGTTGGCCAC TTACAGCGTC 
CGTACCTTTG CCCTCTATAA GAATGGCTCC AGTGAGAAGA GAGAGGTGAG ACAGTTCCAG 
TTCATGGCCT GGCCAGACCA CGGGGTGCCA GAGTACCCCA CCCCAATCCT GGCCTTCCTA 
CGGCGGGTAA AGGCCTGCAA TCCTCCAGAC GCAGGGCCTA TGGTGGTCCA CTGCAGCGCG 
GGTGTGGGCA GGACCGGATG CTTCATCGTG ATAGATGCCA TGCTGGAGCG CATGAAGCAC 
GAGAAGTCCG TAGACATCTA CGGCCACGTG ACGTGCATGC GCGCCCAGAG GAACTACATG 
GTCCAGACCG AGGACCAGTA CATCTTCATT CACGAAGCCC TGCTGGAGGC CGCCACGTGC 
GGCAACACCG AGGTGCCGGC GCGCAACCTG TACGCCCACA TCCAGAAACT CACCCAGCCC 
CCGCCCGGCG AGACGGTTAC TGCCATGGAG CTGGAATTCA AGAGACTGGC CAACTCCAAA 
GCCCACACAT CCCGCTTCAT CAGCGCCAAC TTGCCCTGCA ACAAGTTCAA GAACCGCCTG 
GTCAACATCA TGCCTTTCGA GTCCACCCGC GTCTGCCTGC AGCCCATCCG TGGCGTCGAG 
GGCTCCGACT ACATCAATGC CAGCTGCATC GACGGCTACA GACAGCAGAA GGCCTACCTG 
GCCACGCAGG GCCCACTGGC AGAGACCACC GAGGACTTCT GGAGGATGCT GTGGGAGCAC 
AACTCCACCA TCGTCGTCAT GCTCACCAAG CTACGAGAGA TGGGCCGGGA GAAGTGCCAT 
CAGTACTGGC CAGCAGAGCG TTCAGCCAGG TACCAGTACT TTGTGGTGGA TCCAATGGCT 
GAGTACAACA TGCCCCAGTA CATCCTCAGA GAGTTCAAAG TCACAGATGC CAGGGACGGC 
CAGTCCAGAA CAATCCGCCA ATTCCAGTTC ACTGACTGGC CCGAGCAAGG AGTGCCAAAA 
ACCGGAGAGG GCTTCATTGA TTTTATCGGA CAAGTGCACA AAACCAAGGA ACAGTTCGGA 
CAGGACGGAC CAATCACTGT ACACTGCAGT GCTGGGGTGG GCAGGACCGG AGTCTTCATC 
ACCCTCAGCA TCGTGCTGGA GAGGATGAGG TATGAAGGAG TTGTCGACCT CTTCCAGACA 
GTGAAGACAC TTCGCACCCA GCGGCCTGCC ATGGTGCAGA CCGAGGACCA GTACCAGTTG 
TGCTACAGGG CAGCATTGGA GTACCTGGGG AGCTTTGACC ACTATGCAAC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010744746.2
CDS	492..6245
Translation	MVPNIHLAVPFLPRGALLFLILLSCVVFSSLADSMPSFVKSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVEKTRTATMLCAASGNPDPEISWFKDMLPVDISSSNGRIKQLRSGALQIENSEESDQGKYECVAMNSAGTRYSAPANLYVRVRRVPPRFSIPPTNQEVMPGGSVNLTCVAVGSPMPYVKWTMGLVELTKEEEMPMGRNVLEVNNIRESANYTCVAISSLGMIEATAQVTVKALPKPPASLIVTETTATSVTLTWDSGNPEPVSYYMIQYRAKTSDNGFQEVEGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSDPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRVYYSSDLTAPLSAWQKHNTDDSSLTTISGLTPDITYSLRVLGFTSVGDGPPSDILQVKTQQGVPAQPSSFEAEAELDSRIMLTWLWPVQDPITKYELQYWEAGSDNKLHVSFNPAGSYAVDGLKPDTLYRFSLAARSEMGLGVYTQPIEARTAQSTPSAPPQEVHLVSLSSTSLKVSWVAPPAASRHGAIVRYTVSYQAMAGEDTERHEVTGIGADATSFVLEGLEKWTEYQVWVRAHTDVGPGPESAPVRMRTKEDVPGAPPRKLEVEAINSTAIRVTWKPPLQGKQHGQIRGYQVIFSRLENGEPRGQPNIMDVALPEAQWNIEESTEHEAIIAGLHSETTYSVTVAAYTTKGDGARSKAKVITTTGAVPGKPTMMISTTMGNTALIQWQPPKEMVGELMGYQLQYKRTDEENYTSRELRKIDDHYTVTGLHKGATYVFRLSARNRAGIGEAYVKEISTPEDVPSGFPFNLQVTGLTQSSTELTWDPPLVAERNGKIVHYVVVYRDINSQQNSTNRTSETHMTIQGLNPDTTYDIRVQAFTSKGGGPLSPSIQSRTMSNDFPTLNFGVKAVMKTSVLLTWDLPPNYKSQVPFKILYNQQSVEVQGSLKRRLITQLQPDTDYSFVLTSRGNIAGGLQQQVSIRTAPDLINAKPSTHQPQGSKMTINLPKVQTTTRVRWYYIVVVPVSQSTQGWKNPDEMDLDELFKSSEGPVLRRRRHLDSSKPYIAAKLASLPTTFTLGDEKRYNGFYNKPLSSPQEYLCFVLAVLRSEGADSTTNYMTFSASPYSDPVHVRNSMAQGMEQPEMLWVMGPVLAVVLIIIIVIAILLFKNKQERKRASPLPKDEHSGGVKDSLLANSSDPVEMRRLNYQTPGPSSHRCPNTPRMREHPPIPVVDLADHIERLKANDGLRFSQEYESIDPGQQFTWENSNMEVNKPKNRYANVIAYDHSRVVLTSVDAVPGSDYINANYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRSNTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVTMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRAQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPPPGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYLATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_010746444.3
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010746444.3

ORF Insert Sequence:

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTGGACATC 
AGCAGCAGCA ACGGGCGCAT TAAACAGCTT CGTTCAGGAG CTCTGCAAAT TGAGAACAGC 
GAGGAGTCCG ACCAAGGGAA ATACGAGTGT GTTGCCATGA ACAGCGCTGG GACCCGTTAC 
TCCGCTCCTG CCAATCTCTA CGTACGAGTG CGAAGAGTCC CACCCAGATT CTCCATTCCG 
CCCACCAACC AAGAGGTGAT GCCAGGCGGC AGCGTCAACC TGACATGCGT GGCAGTGGGC 
TCGCCGATGC CCTACGTCAA GTGGACGATG GGCCTGGTGG AGCTGACCAA GGAGGAGGAG 
ATGCCGATGG GACGCAACGT GCTGGAGGTC AACAACATCC GCGAGTCGGC GAACTACACC 
TGCGTGGCCA TCTCCTCTCT GGGCATGATA GAAGCCACCG CTCAGGTCAC CGTCAAAGCT 
TTGCCAAAGC CTCCCGCCTC CCTCATTGTG ACTGAGACCA CAGCCACCAG TGTGACTCTC 
ACCTGGGACT CTGGAAACCC AGAACCTGTC TCCTACTACA TGATCCAATA CAGAGCCAAG 
ACCTCGGACA ACGGCTTCCA GGAAGTGGAA GGTGTGGCAA CTACCCGTTA CAGCATCGGT 
GGACTTAGCC CCTACTCTGA GTATGAGTTT CGTGTCATGG CTGTCAACAA CATCGGCCGG 
GGGCCACCGA GCGACCCCGT GGAGACACGC ACTGGTGAAC AGGCCCCCTC CTCGCCACCT 
CTGCACGTCC AAGCGCGCAT GCTGAGTGCC AGCACAATGC TGGTCCAGTG GGAACCGCCC 
GAGGAACCCA ACGGGCAAAT CAGGGGCTAC CGGGTCTACT ACAGCTCGGA CTTGACGGCT 
CCGCTCAGCG CCTGGCAGAA GCACAACACA GACGACAGCA GCCTGACGAC CATCTCGGGC 
CTGACTCCTG ATATCACCTA CAGCCTCAGG GTGCTGGGCT TCACCTCTGT AGGTGACGGG 
CCACCTTCTG ACATCCTGCA AGTCAAAACC CAACAGGGAG TCCCTGCCCA GCCGTCCAGC 
TTTGAGGCCG AAGCTGAGCT GGACAGTCGC ATCATGCTAA CATGGCTGTG GCCCGTCCAG 
GACCCCATCA CTAAGTATGA GCTGCAGTAC TGGGAGGCCG GCTCAGACAA CAAGTTACAT 
GTGAGCTTCA ACCCAGCGGG CTCCTACGCT GTGGACGGTC TAAAGCCTGA CACCTTGTAC 
CGTTTCTCTC TGGCAGCCCG TTCTGAGATG GGCTTAGGTG TCTACACCCA ACCCATCGAG 
GCTCGCACCG CCCAGTCCAC CCCATCAGCT CCCCCCCAGG AGGTACACCT GGTCAGCCTG 
AGCTCCACCA GCCTCAAGGT GAGTTGGGTG GCACCGCCCG CCGCTAGCCG CCACGGCGCC 
ATTGTGCGCT ACACAGTCAG CTACCAGGCC ATGGCTGGAG AAGACACGGA GCGGCACGAG 
GTGACGGGCA TCGGCGCAGA CGCCACTAGC TTTGTGCTGG AGGGCCTGGA GAAGTGGACT 
GAGTATCAGG TGTGGGTGAG GGCACACACT GACGTGGGCC CCGGCCCAGA GAGTGCCCCA 
GTGAGGATGA GAACCAAAGA GGATGTGCCG GGAGCACCCC CCAGGAAGCT GGAGGTTGAG 
GCCATAAACT CCACAGCAAT CCGGGTCACC TGGAAGCCGC CCCTGCAGGG GAAGCAGCAC 
GGCCAAATCC GGGGCTATCA AGTCATCTTC TCTCGGCTTG AAAACGGCGA ACCACGAGGC 
CAGCCTAACA TCATGGACGT TGCTTTGCCA GAGGCACAGT GGAATATTGA GGAGTCCACC 
GAGCATGAGG CCATTATTGC CGGACTGCAC TCTGAAACCA CCTACTCGGT CACCGTGGCA 
GCATACACCA CCAAAGGAGA CGGAGCGCGC AGCAAAGCCA AAGTCATCAC CACGACGGGT 
GCAGTGCCAG GAAAGCCCAC CATGATGATC AGCACCACTA TGGGCAACAC AGCGCTGATT 
CAGTGGCAGC CGCCTAAGGA AATGGTTGGG GAGCTGATGG GCTACCAGCT GCAGTACAAG 
CGCACTGACG AGGAAAACTA CACCTCCCGC GAGCTGAGAA AGATCGATGA CCATTACACC 
GTCACCGGGC TGCACAAAGG TGCCACCTAT GTCTTCCGCC TCAGTGCCCG CAACCGCGCG 
GGCATCGGCG AAGCATACGT GAAAGAGATC AGCACCCCTG AAGACGTGCC GTCGGGCTTC 
CCGTTCAACC TGCAGGTCAC GGGACTGACT CAGTCCAGCA CCGAGCTGAC CTGGGACCCT 
CCGCTGGTGG CCGAAAGGAA CGGCAAGATC GTGCACTATG TGGTCGTTTA CAGGGACATC 
AACAGTCAGC AGAACAGCAC CAACCGGACG AGCGAAACGC ACATGACCAT CCAGGGCCTT 
AATCCCGACA CCACCTACGA TATCCGGGTG CAGGCGTTCA CCAGTAAGGG CGGAGGACCC 
CTCAGCCCTA GCATCCAGAG CAGGACCATG TCGAACGATT TTCCCACCCT CAACTTTGGT 
GTCAAAGCCG TCATGAAAAC ATCTGTCCTC CTCACCTGGG ATTTGCCACC GAACTACAAA 
TCCCAAGTGC CTTTCAAGAT CCTGTACAAC CAGCAAAGCG TGGAAGTGCA GGGATCCCTG 
AAGAGGAGGC TGATCACGCA GCTGCAGCCA GACACGGACT ACTCTTTTGT GCTGACAAGC 
AGGGGCAACA TTGCTGGTGG TCTACAGCAG CAGGTGTCCA TCCGCACCGC TCCCGACCTG 
ATCAACGCCA AGCCCAGCAC ACATCAGCCT CAGGGTAGCA AGATGACAAT CAACCTGCCC 
AAGGTCCAGA CCACCACGCG TGTCAGGTGG TACTACATCG TGGTGGTGCC GGTGTCCCAG 
TCAACGCAGG GGTGGAAGAA TCCAGACGAG ATGGACCTGG ACGAGCTGTT CAAGTCAAGT 
GAAGGCCCCG TCCTGAGGCG CAGACGTCAC CTGGATTCCT CCAAACCTTA TATCGCTGCC 
AAGTTGGCCT CGTTGCCGAC CACCTTCACC CTGGGTGATG AGAAGAGATA CAACGGCTTC 
TACAACAAGC CCCTGAGCAG TCCGCAGGAG TATCTCTGCT TTGTCCTGGC AGTACTCCGA 
TCCGAAGGGG CAGACAGCAC TACTAATTAT ATGACATTTT CAGCCAGTCC CTACTCAGAC 
CCGGTCCACG TGCGAAACAG CATGGCTCAG GGGATGGAGC AGCCTGAAAT GTTGTGGGTT 
ATGGGCCCTG TTTTGGCCGT GGTCCTGATT ATCATCATCG TCATCGCCAT CCTGCTCTTC 
AAGAACAAAC AGGAAAGGAA GCGAGCGTCG CCCCTACCCA AAGATGAGCA CTCGGGCGGG 
GTGAAGGACT CCCTGCTGGC CAACTCCTCC GACCCTGTGG AGATGAGAAG ACTTAACTAC 
CAGACCCCAG GTCCCAGCTC TCATCGCTGC CCCAACACTC CAAGAATGAG GGAGCACCCG 
CCCATCCCTG TCGTCGACCT GGCTGACCAC ATAGAGCGTC TGAAGGCCAA CGACGGCCTA 
CGCTTTTCCC AGGAGTACGA GTCGATTGAT CCAGGGCAGC AGTTTACCTG GGAGAACTCC 
AACATGGAAG TCAACAAGCC AAAGAATCGC TATGCAAACG TCATCGCCTA TGACCACTCC 
AGAGTGGTTC TCACCTCTGT TGACGCCGTT CCAGGCAGTG ACTACATCAA CGCCAACTAC 
ATCGACGGCT ACAGGAAGCA GAACGCCTAC ATCGCCACGC AGGGGCCACT TCCAGAGACA 
CTGAGCGACT TCTGGAGGAT GGTGTGGGAG CAAAGATCCA ACACCATCGT CATGATGACC 
AGACTGGAGG AGAAGTCGCG GGTGAAGTGT GACCAGTACT GGCCGTCGCG AGGAACGGAG 
ACCTATGGGA TGATCCAGGT CACCATGCTG GACACCGTGG AGTTGGCCAC TTACAGCGTC 
CGTACCTTTG CCCTCTATAA GAATGGCTCC AGTGAGAAGA GAGAGGTGAG ACAGTTCCAG 
TTCATGGCCT GGCCAGACCA CGGGGTGCCA GAGTACCCCA CCCCAATCCT GGCCTTCCTA 
CGGCGGGTAA AGGCCTGCAA TCCTCCAGAC GCAGGGCCTA TGGTGGTCCA CTGCAGCGCG 
GGTGTGGGCA GGACCGGATG CTTCATCGTG ATAGATGCCA TGCTGGAGCG CATGAAGCAC 
GAGAAGTCCG TAGACATCTA CGGCCACGTG ACGTGCATGC GCGCCCAGAG GAACTACATG 
GTCCAGACCG AGGACCAGTA CATCTTCATT CACGAAGCCC TGCTGGAGGC CGCCACGTGC 
GGCAACACCG AGGTGCCGGC GCGCAACCTG TACGCCCACA TCCAGAAACT CACCCAGCCC 
CCGCCCGGCG AGACGGTTAC TGCCATGGAG CTGGAATTCA AGAGACTGGC CAACTCCAAA 
GCCCACACAT CCCGCTTCAT CAGCGCCAAC TTGCCCTGCA ACAAGTTCAA GAACCGCCTG 
GTCAACATCA TGCCTTTCGA GTCCACCCGC GTCTGCCTGC AGCCCATCCG TGGCGTCGAG 
GGCTCCGACT ACATCAATGC CAGCTGCATC GACGGCTACA GACAGCAGAA GGCCTACCTG 
GCCACGCAGG GCCCACTGGC AGAGACCACC GAGGACTTCT GGAGGATGCT GTGGGAGCAC 
AACTCCACCA TCGTCGTCAT GCTCACCAAG CTACGAGAGA TGGGCCGGGA GAAGTGCCAT 
CAGTACTGGC CAGCAGAGCG TTCAGCCAGG TACCAGTACT TTGTGGTGGA TCCAATGGCT 
GAGTACAACA TGCCCCAGTA CATCCTCAGA GAGTTCAAAG TCACAGATGC CAGGGACGGC 
CAGTCCAGAA CAATCCGCCA ATTCCAGTTC ACTGACTGGC CCGAGCAAGG AGTGCCAAAA 
ACCGGAGAGG GCTTCATTGA TTTTATCGGA CAAGTGCACA AAACCAAGGA ACAGTTCGGA 
CAGGACGGAC CAATCACTGT ACACTGCAGT GCTGGGGTGG GCAGGACCGG AGTCTTCATC 
ACCCTCAGCA TCGTGCTGGA GAGGATGAGG TATGAAGGAG TTGTCGACCT CTTCCAGACA 
GTGAAGACAC TTCGCACCCA GCGGCCTGCC ATGGTGCAGA CCGAGGACCA GTACCAGTTG 
TGCTACAGGG CAGCATTGGA GTACCTGGGG AGCTTTGACC ACTATGCAAC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104931439 ( XM_019263539.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019119084.1 Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X5, mRNA.

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CloneID	OLa211349
Clone ID Related Accession (Same CDS sequence)	XM_019263539.2
Accession Version	XM_019263539.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	receptor-type tyrosine-protein phosphatase F isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040012.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_019263539.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTGGACATC 
AGCAGCAGCA ACGGGCGCAT TAAACAGCTT CGTTCAGGTG GTACACCAAT TAGAGGAGCT 
CTGCAAATTG AGAACAGCGA GGAGTCCGAC CAAGGGAAAT ACGAGTGTGT TGCCATGAAC 
AGCGCTGGGA CCCGTTACTC CGCTCCTGCC AATCTCTACG TACGAGTGCG AAGAGTCCCA 
CCCAGATTCT CCATTCCGCC CACCAACCAA GAGGTGATGC CAGGCGGCAG CGTCAACCTG 
ACATGCGTGG CAGTGGGCTC GCCGATGCCC TACGTCAAGT GGACGATGGG CCTGGTGGAG 
CTGACCAAGG AGGAGGAGAT GCCGATGGGA CGCAACGTGC TGGAGGTCAA CAACATCCGC 
GAGTCGGCGA ACTACACCTG CGTGGCCATC TCCTCTCTGG GCATGATAGA AGCCACCGCT 
CAGGTCACCG TCAAAGCTTT GCCAAAGCCT CCCGCCTCCC TCATTGTGAC TGAGACCACA 
GCCACCAGTG TGACTCTCAC CTGGGACTCT GGAAACCCAG AACCTGTCTC CTACTACATG 
ATCCAATACA GAGCCAAGAC CTCGGACAAC GGCTTCCAGG AAGTGGAAGG TGTGGCAACT 
ACCCGTTACA GCATCGGTGG ACTTAGCCCC TACTCTGAGT ATGAGTTTCG TGTCATGGCT 
GTCAACAACA TCGGCCGGGG GCCACCGAGC GACCCCGTGG AGACACGCAC TGGTGAACAG 
GCCCCCTCCT CGCCACCTCT GCACGTCCAA GCGCGCATGC TGAGTGCCAG CACAATGCTG 
GTCCAGTGGG AACCGCCCGA GGAACCCAAC GGGCAAATCA GGGGCTACCG GGTCTACTAC 
AGCTCGGACT TGACGGCTCC GCTCAGCGCC TGGCAGAAGC ACAACACAGA CGACAGCAGC 
CTGACGACCA TCTCGGGCCT GACTCCTGAT ATCACCTACA GCCTCAGGGT GCTGGGCTTC 
ACCTCTGTAG GTGACGGGCC ACCTTCTGAC ATCCTGCAAG TCAAAACCCA ACAGGGAGTC 
CCTGCCCAGC CGTCCAGCTT TGAGGCCGAA GCTGAGCTGG ACAGTCGCAT CATGCTAACA 
TGGCTGTGGC CCGTCCAGGA CCCCATCACT AAGTATGAGC TGCAGTACTG GGAGGCCGGC 
TCAGACAACA AGTTACATGT GAGCTTCAAC CCAGCGGGCT CCTACGCTGT GGACGGTCTA 
AAGCCTGACA CCTTGTACCG TTTCTCTCTG GCAGCCCGTT CTGAGATGGG CTTAGGTGTC 
TACACCCAAC CCATCGAGGC TCGCACCGCC CAGTCCACCC CATCAGCTCC CCCCCAGGAG 
GTACACCTGG TCAGCCTGAG CTCCACCAGC CTCAAGGTGA GTTGGGTGGC ACCGCCCGCC 
GCTAGCCGCC ACGGCGCCAT TGTGCGCTAC ACAGTCAGCT ACCAGGCCAT GGCTGGAGAA 
GACACGGAGC GGCACGAGGT GACGGGCATC GGCGCAGACG CCACTAGCTT TGTGCTGGAG 
GGCCTGGAGA AGTGGACTGA GTATCAGGTG TGGGTGAGGG CACACACTGA CGTGGGCCCC 
GGCCCAGAGA GTGCCCCAGT GAGGATGAGA ACCAAAGAGG ATGTGCCGGG AGCACCCCCC 
AGGAAGCTGG AGGTTGAGGC CATAAACTCC ACAGCAATCC GGGTCACCTG GAAGCCGCCC 
CTGCAGGGGA AGCAGCACGG CCAAATCCGG GGCTATCAAG TCATCTTCTC TCGGCTTGAA 
AACGGCGAAC CACGAGGCCA GCCTAACATC ATGGACGTTG CTTTGCCAGA GGCACAGGAG 
GCCATTATTG CCGGACTGCA CTCTGAAACC ACCTACTCGG TCACCGTGGC AGCATACACC 
ACCAAAGGAG ACGGAGCGCG CAGCAAAGCC AAAGTCATCA CCACGACGGG TGCAGTGCCA 
GGAAAGCCCA CCATGATGAT CAGCACCACT ATGGGCAACA CAGCGCTGAT TCAGTGGCAG 
CCGCCTAAGG AAATGGTTGG GGAGCTGATG GGCTACCAGC TGCAGTACAA GCGCACTGAC 
GAGGAAAACT ACACCTCCCG CGAGCTGAGA AAGATCGATG ACCATTACAC CGTCACCGGG 
CTGCACAAAG GTGCCACCTA TGTCTTCCGC CTCAGTGCCC GCAACCGCGC GGGCATCGGC 
GAAGCATACG TGAAAGAGAT CAGCACCCCT GAAGACGTGC CGTCGGGCTT CCCGTTCAAC 
CTGCAGGTCA CGGGACTGAC TCAGTCCAGC ACCGAGCTGA CCTGGGACCC TCCGCTGGTG 
GCCGAAAGGA ACGGCAAGAT CGTGCACTAT GTGGTCGTTT ACAGGGACAT CAACAGTCAG 
CAGAACAGCA CCAACCGGAC GAGCGAAACG CACATGACCA TCCAGGGCCT TAATCCCGAC 
ACCACCTACG ATATCCGGGT GCAGGCGTTC ACCAGTAAGG GCGGAGGACC CCTCAGCCCT 
AGCATCCAGA GCAGGACCAT GTCGAACGAT TTTCCCACCC TCAACTTTGG TGTCAAAGCC 
GTCATGAAAA CATCTGTCCT CCTCACCTGG GATTTGCCAC CGAACTACAA ATCCCAAGTG 
CCTTTCAAGA TCCTGTACAA CCAGCAAAGC GTGGAAGTGC AGGGATCCCT GAAGAGGAGG 
CTGATCACGC AGCTGCAGCC AGACACGGAC TACTCTTTTG TGCTGACAAG CAGGGGCAAC 
ATTGCTGGTG GTCTACAGCA GCAGGTGTCC ATCCGCACCG CTCCCGACCT GATCAACGCC 
AAGCCCAGCA CACATCAGCC TCAGGGTAGC AAGATGACAA TCAACCTGCC CAAGGTCCAG 
ACCACCACGC GTGTCAGGTG GTACTACATC GTGGTGGTGC CGGTGTCCCA GTCAACGCAG 
GGGTGGAAGA ATCCAGACGA GATGGACCTG GACGAGCTGT TCAAGTCAAG TGAAGGCCCC 
GTCCTGAGGC GCAGACGTCA CCTGGATTCC TCCAAACCTT ATATCGCTGC CAAGTTGGCC 
TCGTTGCCGA CCACCTTCAC CCTGGGTGAT GAGAAGAGAT ACAACGGCTT CTACAACAAG 
CCCCTGAGCA GTCCGCAGGA GTATCTCTGC TTTGTCCTGG CAGTACTCCG ATCCGAAGGG 
GCAGACAGCA CTACTAATTA TATGACATTT TCAGCCAGTC CCTACTCAGA CCCGGTCCAC 
GTGCGAAACA GCATGGCTCA GGGGATGGAG CAGCCTGAAA TGTTGTGGGT TATGGGCCCT 
GTTTTGGCCG TGGTCCTGAT TATCATCATC GTCATCGCCA TCCTGCTCTT CAAGAACAAA 
CAGGAAAGGA AGCGAGCGTC GCCCCTACCC AAAGATGAGC ACTCGGGCGG GGTGAAGGAC 
TCCCTGCTGG CCAACTCCTC CGACCCTGTG GAGATGAGAA GACTTAACTA CCAGACCCCA 
GGTCCCAGCT CTCATCGCTG CCCCAACACT CCAAGAATGA GGGAGCACCC GCCCATCCCT 
GTCGTCGACC TGGCTGACCA CATAGAGCGT CTGAAGGCCA ACGACGGCCT ACGCTTTTCC 
CAGGAGTACG AGTCGATTGA TCCAGGGCAG CAGTTTACCT GGGAGAACTC CAACATGGAA 
GTCAACAAGC CAAAGAATCG CTATGCAAAC GTCATCGCCT ATGACCACTC CAGAGTGGTT 
CTCACCTCTG TTGACGCCGT TCCAGGCAGT GACTACATCA ACGCCAACTA CATCGACGGC 
TACAGGAAGC AGAACGCCTA CATCGCCACG CAGGGGCCAC TTCCAGAGAC ACTGAGCGAC 
TTCTGGAGGA TGGTGTGGGA GCAAAGATCC AACACCATCG TCATGATGAC CAGACTGGAG 
GAGAAGTCGC GGGTGAAGTG TGACCAGTAC TGGCCGTCGC GAGGAACGGA GACCTATGGG 
ATGATCCAGG TCACCATGCT GGACACCGTG GAGTTGGCCA CTTACAGCGT CCGTACCTTT 
GCCCTCTATA AGAATGGCTC CAGTGAGAAG AGAGAGGTGA GACAGTTCCA GTTCATGGCC 
TGGCCAGACC ACGGGGTGCC AGAGTACCCC ACCCCAATCC TGGCCTTCCT ACGGCGGGTA 
AAGGCCTGCA ATCCTCCAGA CGCAGGGCCT ATGGTGGTCC ACTGCAGCGC GGGTGTGGGC 
AGGACCGGAT GCTTCATCGT GATAGATGCC ATGCTGGAGC GCATGAAGCA CGAGAAGTCC 
GTAGACATCT ACGGCCACGT GACGTGCATG CGCGCCCAGA GGAACTACAT GGTCCAGACC 
GAGGACCAGT ACATCTTCAT TCACGAAGCC CTGCTGGAGG CCGCCACGTG CGGCAACACC 
GAGGTGCCGG CGCGCAACCT GTACGCCCAC ATCCAGAAAC TCACCCAGCC CCCGCCCGGC 
GAGACGGTTA CTGCCATGGA GCTGGAATTC AAGAGACTGG CCAACTCCAA AGCCCACACA 
TCCCGCTTCA TCAGCGCCAA CTTGCCCTGC AACAAGTTCA AGAACCGCCT GGTCAACATC 
ATGCCTTTCG AGTCCACCCG CGTCTGCCTG CAGCCCATCC GTGGCGTCGA GGGCTCCGAC 
TACATCAATG CCAGCTGCAT CGACGGCTAC AGACAGCAGA AGGCCTACCT GGCCACGCAG 
GGCCCACTGG CAGAGACCAC CGAGGACTTC TGGAGGATGC TGTGGGAGCA CAACTCCACC 
ATCGTCGTCA TGCTCACCAA GCTACGAGAG ATGGGCCGGG AGAAGTGCCA TCAGTACTGG 
CCAGCAGAGC GTTCAGCCAG GTACCAGTAC TTTGTGGTGG ATCCAATGGC TGAGTACAAC 
ATGCCCCAGT ACATCCTCAG AGAGTTCAAA GTCACAGATG CCAGGGACGG CCAGTCCAGA 
ACAATCCGCC AATTCCAGTT CACTGACTGG CCCGAGCAAG GAGTGCCAAA AACCGGAGAG 
GGCTTCATTG ATTTTATCGG ACAAGTGCAC AAAACCAAGG AACAGTTCGG ACAGGACGGA 
CCAATCACTG TACACTGCAG TGCTGGGGTG GGCAGGACCG GAGTCTTCAT CACCCTCAGC 
ATCGTGCTGG AGAGGATGAG GTATGAAGGA GTTGTCGACC TCTTCCAGAC AGTGAAGACA 
CTTCGCACCC AGCGGCCTGC CATGGTGCAG ACCGAGGACC AGTACCAGTT GTGCTACAGG 
GCAGCATTGG AGTACCTGGG GAGCTTTGAC CACTATGCAA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019119084.1
CDS	493..6237
Translation	MVPNIHLAVPFLPRGALLFLILLSCVVFSSLADSMPSFVKSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVEKTRTATMLCAASGNPDPEISWFKDMLPVDISSSNGRIKQLRSGGTPIRGALQIENSEESDQGKYECVAMNSAGTRYSAPANLYVRVRRVPPRFSIPPTNQEVMPGGSVNLTCVAVGSPMPYVKWTMGLVELTKEEEMPMGRNVLEVNNIRESANYTCVAISSLGMIEATAQVTVKALPKPPASLIVTETTATSVTLTWDSGNPEPVSYYMIQYRAKTSDNGFQEVEGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSDPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRVYYSSDLTAPLSAWQKHNTDDSSLTTISGLTPDITYSLRVLGFTSVGDGPPSDILQVKTQQGVPAQPSSFEAEAELDSRIMLTWLWPVQDPITKYELQYWEAGSDNKLHVSFNPAGSYAVDGLKPDTLYRFSLAARSEMGLGVYTQPIEARTAQSTPSAPPQEVHLVSLSSTSLKVSWVAPPAASRHGAIVRYTVSYQAMAGEDTERHEVTGIGADATSFVLEGLEKWTEYQVWVRAHTDVGPGPESAPVRMRTKEDVPGAPPRKLEVEAINSTAIRVTWKPPLQGKQHGQIRGYQVIFSRLENGEPRGQPNIMDVALPEAQEAIIAGLHSETTYSVTVAAYTTKGDGARSKAKVITTTGAVPGKPTMMISTTMGNTALIQWQPPKEMVGELMGYQLQYKRTDEENYTSRELRKIDDHYTVTGLHKGATYVFRLSARNRAGIGEAYVKEISTPEDVPSGFPFNLQVTGLTQSSTELTWDPPLVAERNGKIVHYVVVYRDINSQQNSTNRTSETHMTIQGLNPDTTYDIRVQAFTSKGGGPLSPSIQSRTMSNDFPTLNFGVKAVMKTSVLLTWDLPPNYKSQVPFKILYNQQSVEVQGSLKRRLITQLQPDTDYSFVLTSRGNIAGGLQQQVSIRTAPDLINAKPSTHQPQGSKMTINLPKVQTTTRVRWYYIVVVPVSQSTQGWKNPDEMDLDELFKSSEGPVLRRRRHLDSSKPYIAAKLASLPTTFTLGDEKRYNGFYNKPLSSPQEYLCFVLAVLRSEGADSTTNYMTFSASPYSDPVHVRNSMAQGMEQPEMLWVMGPVLAVVLIIIIVIAILLFKNKQERKRASPLPKDEHSGGVKDSLLANSSDPVEMRRLNYQTPGPSSHRCPNTPRMREHPPIPVVDLADHIERLKANDGLRFSQEYESIDPGQQFTWENSNMEVNKPKNRYANVIAYDHSRVVLTSVDAVPGSDYINANYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRSNTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVTMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRAQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPPPGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYLATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_019263539.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019263539.2

ORF Insert Sequence:

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTGGACATC 
AGCAGCAGCA ACGGGCGCAT TAAACAGCTT CGTTCAGGTG GTACACCAAT TAGAGGAGCT 
CTGCAAATTG AGAACAGCGA GGAGTCCGAC CAAGGGAAAT ACGAGTGTGT TGCCATGAAC 
AGCGCTGGGA CCCGTTACTC CGCTCCTGCC AATCTCTACG TACGAGTGCG AAGAGTCCCA 
CCCAGATTCT CCATTCCGCC CACCAACCAA GAGGTGATGC CAGGCGGCAG CGTCAACCTG 
ACATGCGTGG CAGTGGGCTC GCCGATGCCC TACGTCAAGT GGACGATGGG CCTGGTGGAG 
CTGACCAAGG AGGAGGAGAT GCCGATGGGA CGCAACGTGC TGGAGGTCAA CAACATCCGC 
GAGTCGGCGA ACTACACCTG CGTGGCCATC TCCTCTCTGG GCATGATAGA AGCCACCGCT 
CAGGTCACCG TCAAAGCTTT GCCAAAGCCT CCCGCCTCCC TCATTGTGAC TGAGACCACA 
GCCACCAGTG TGACTCTCAC CTGGGACTCT GGAAACCCAG AACCTGTCTC CTACTACATG 
ATCCAATACA GAGCCAAGAC CTCGGACAAC GGCTTCCAGG AAGTGGAAGG TGTGGCAACT 
ACCCGTTACA GCATCGGTGG ACTTAGCCCC TACTCTGAGT ATGAGTTTCG TGTCATGGCT 
GTCAACAACA TCGGCCGGGG GCCACCGAGC GACCCCGTGG AGACACGCAC TGGTGAACAG 
GCCCCCTCCT CGCCACCTCT GCACGTCCAA GCGCGCATGC TGAGTGCCAG CACAATGCTG 
GTCCAGTGGG AACCGCCCGA GGAACCCAAC GGGCAAATCA GGGGCTACCG GGTCTACTAC 
AGCTCGGACT TGACGGCTCC GCTCAGCGCC TGGCAGAAGC ACAACACAGA CGACAGCAGC 
CTGACGACCA TCTCGGGCCT GACTCCTGAT ATCACCTACA GCCTCAGGGT GCTGGGCTTC 
ACCTCTGTAG GTGACGGGCC ACCTTCTGAC ATCCTGCAAG TCAAAACCCA ACAGGGAGTC 
CCTGCCCAGC CGTCCAGCTT TGAGGCCGAA GCTGAGCTGG ACAGTCGCAT CATGCTAACA 
TGGCTGTGGC CCGTCCAGGA CCCCATCACT AAGTATGAGC TGCAGTACTG GGAGGCCGGC 
TCAGACAACA AGTTACATGT GAGCTTCAAC CCAGCGGGCT CCTACGCTGT GGACGGTCTA 
AAGCCTGACA CCTTGTACCG TTTCTCTCTG GCAGCCCGTT CTGAGATGGG CTTAGGTGTC 
TACACCCAAC CCATCGAGGC TCGCACCGCC CAGTCCACCC CATCAGCTCC CCCCCAGGAG 
GTACACCTGG TCAGCCTGAG CTCCACCAGC CTCAAGGTGA GTTGGGTGGC ACCGCCCGCC 
GCTAGCCGCC ACGGCGCCAT TGTGCGCTAC ACAGTCAGCT ACCAGGCCAT GGCTGGAGAA 
GACACGGAGC GGCACGAGGT GACGGGCATC GGCGCAGACG CCACTAGCTT TGTGCTGGAG 
GGCCTGGAGA AGTGGACTGA GTATCAGGTG TGGGTGAGGG CACACACTGA CGTGGGCCCC 
GGCCCAGAGA GTGCCCCAGT GAGGATGAGA ACCAAAGAGG ATGTGCCGGG AGCACCCCCC 
AGGAAGCTGG AGGTTGAGGC CATAAACTCC ACAGCAATCC GGGTCACCTG GAAGCCGCCC 
CTGCAGGGGA AGCAGCACGG CCAAATCCGG GGCTATCAAG TCATCTTCTC TCGGCTTGAA 
AACGGCGAAC CACGAGGCCA GCCTAACATC ATGGACGTTG CTTTGCCAGA GGCACAGGAG 
GCCATTATTG CCGGACTGCA CTCTGAAACC ACCTACTCGG TCACCGTGGC AGCATACACC 
ACCAAAGGAG ACGGAGCGCG CAGCAAAGCC AAAGTCATCA CCACGACGGG TGCAGTGCCA 
GGAAAGCCCA CCATGATGAT CAGCACCACT ATGGGCAACA CAGCGCTGAT TCAGTGGCAG 
CCGCCTAAGG AAATGGTTGG GGAGCTGATG GGCTACCAGC TGCAGTACAA GCGCACTGAC 
GAGGAAAACT ACACCTCCCG CGAGCTGAGA AAGATCGATG ACCATTACAC CGTCACCGGG 
CTGCACAAAG GTGCCACCTA TGTCTTCCGC CTCAGTGCCC GCAACCGCGC GGGCATCGGC 
GAAGCATACG TGAAAGAGAT CAGCACCCCT GAAGACGTGC CGTCGGGCTT CCCGTTCAAC 
CTGCAGGTCA CGGGACTGAC TCAGTCCAGC ACCGAGCTGA CCTGGGACCC TCCGCTGGTG 
GCCGAAAGGA ACGGCAAGAT CGTGCACTAT GTGGTCGTTT ACAGGGACAT CAACAGTCAG 
CAGAACAGCA CCAACCGGAC GAGCGAAACG CACATGACCA TCCAGGGCCT TAATCCCGAC 
ACCACCTACG ATATCCGGGT GCAGGCGTTC ACCAGTAAGG GCGGAGGACC CCTCAGCCCT 
AGCATCCAGA GCAGGACCAT GTCGAACGAT TTTCCCACCC TCAACTTTGG TGTCAAAGCC 
GTCATGAAAA CATCTGTCCT CCTCACCTGG GATTTGCCAC CGAACTACAA ATCCCAAGTG 
CCTTTCAAGA TCCTGTACAA CCAGCAAAGC GTGGAAGTGC AGGGATCCCT GAAGAGGAGG 
CTGATCACGC AGCTGCAGCC AGACACGGAC TACTCTTTTG TGCTGACAAG CAGGGGCAAC 
ATTGCTGGTG GTCTACAGCA GCAGGTGTCC ATCCGCACCG CTCCCGACCT GATCAACGCC 
AAGCCCAGCA CACATCAGCC TCAGGGTAGC AAGATGACAA TCAACCTGCC CAAGGTCCAG 
ACCACCACGC GTGTCAGGTG GTACTACATC GTGGTGGTGC CGGTGTCCCA GTCAACGCAG 
GGGTGGAAGA ATCCAGACGA GATGGACCTG GACGAGCTGT TCAAGTCAAG TGAAGGCCCC 
GTCCTGAGGC GCAGACGTCA CCTGGATTCC TCCAAACCTT ATATCGCTGC CAAGTTGGCC 
TCGTTGCCGA CCACCTTCAC CCTGGGTGAT GAGAAGAGAT ACAACGGCTT CTACAACAAG 
CCCCTGAGCA GTCCGCAGGA GTATCTCTGC TTTGTCCTGG CAGTACTCCG ATCCGAAGGG 
GCAGACAGCA CTACTAATTA TATGACATTT TCAGCCAGTC CCTACTCAGA CCCGGTCCAC 
GTGCGAAACA GCATGGCTCA GGGGATGGAG CAGCCTGAAA TGTTGTGGGT TATGGGCCCT 
GTTTTGGCCG TGGTCCTGAT TATCATCATC GTCATCGCCA TCCTGCTCTT CAAGAACAAA 
CAGGAAAGGA AGCGAGCGTC GCCCCTACCC AAAGATGAGC ACTCGGGCGG GGTGAAGGAC 
TCCCTGCTGG CCAACTCCTC CGACCCTGTG GAGATGAGAA GACTTAACTA CCAGACCCCA 
GGTCCCAGCT CTCATCGCTG CCCCAACACT CCAAGAATGA GGGAGCACCC GCCCATCCCT 
GTCGTCGACC TGGCTGACCA CATAGAGCGT CTGAAGGCCA ACGACGGCCT ACGCTTTTCC 
CAGGAGTACG AGTCGATTGA TCCAGGGCAG CAGTTTACCT GGGAGAACTC CAACATGGAA 
GTCAACAAGC CAAAGAATCG CTATGCAAAC GTCATCGCCT ATGACCACTC CAGAGTGGTT 
CTCACCTCTG TTGACGCCGT TCCAGGCAGT GACTACATCA ACGCCAACTA CATCGACGGC 
TACAGGAAGC AGAACGCCTA CATCGCCACG CAGGGGCCAC TTCCAGAGAC ACTGAGCGAC 
TTCTGGAGGA TGGTGTGGGA GCAAAGATCC AACACCATCG TCATGATGAC CAGACTGGAG 
GAGAAGTCGC GGGTGAAGTG TGACCAGTAC TGGCCGTCGC GAGGAACGGA GACCTATGGG 
ATGATCCAGG TCACCATGCT GGACACCGTG GAGTTGGCCA CTTACAGCGT CCGTACCTTT 
GCCCTCTATA AGAATGGCTC CAGTGAGAAG AGAGAGGTGA GACAGTTCCA GTTCATGGCC 
TGGCCAGACC ACGGGGTGCC AGAGTACCCC ACCCCAATCC TGGCCTTCCT ACGGCGGGTA 
AAGGCCTGCA ATCCTCCAGA CGCAGGGCCT ATGGTGGTCC ACTGCAGCGC GGGTGTGGGC 
AGGACCGGAT GCTTCATCGT GATAGATGCC ATGCTGGAGC GCATGAAGCA CGAGAAGTCC 
GTAGACATCT ACGGCCACGT GACGTGCATG CGCGCCCAGA GGAACTACAT GGTCCAGACC 
GAGGACCAGT ACATCTTCAT TCACGAAGCC CTGCTGGAGG CCGCCACGTG CGGCAACACC 
GAGGTGCCGG CGCGCAACCT GTACGCCCAC ATCCAGAAAC TCACCCAGCC CCCGCCCGGC 
GAGACGGTTA CTGCCATGGA GCTGGAATTC AAGAGACTGG CCAACTCCAA AGCCCACACA 
TCCCGCTTCA TCAGCGCCAA CTTGCCCTGC AACAAGTTCA AGAACCGCCT GGTCAACATC 
ATGCCTTTCG AGTCCACCCG CGTCTGCCTG CAGCCCATCC GTGGCGTCGA GGGCTCCGAC 
TACATCAATG CCAGCTGCAT CGACGGCTAC AGACAGCAGA AGGCCTACCT GGCCACGCAG 
GGCCCACTGG CAGAGACCAC CGAGGACTTC TGGAGGATGC TGTGGGAGCA CAACTCCACC 
ATCGTCGTCA TGCTCACCAA GCTACGAGAG ATGGGCCGGG AGAAGTGCCA TCAGTACTGG 
CCAGCAGAGC GTTCAGCCAG GTACCAGTAC TTTGTGGTGG ATCCAATGGC TGAGTACAAC 
ATGCCCCAGT ACATCCTCAG AGAGTTCAAA GTCACAGATG CCAGGGACGG CCAGTCCAGA 
ACAATCCGCC AATTCCAGTT CACTGACTGG CCCGAGCAAG GAGTGCCAAA AACCGGAGAG 
GGCTTCATTG ATTTTATCGG ACAAGTGCAC AAAACCAAGG AACAGTTCGG ACAGGACGGA 
CCAATCACTG TACACTGCAG TGCTGGGGTG GGCAGGACCG GAGTCTTCAT CACCCTCAGC 
ATCGTGCTGG AGAGGATGAG GTATGAAGGA GTTGTCGACC TCTTCCAGAC AGTGAAGACA 
CTTCGCACCC AGCGGCCTGC CATGGTGCAG ACCGAGGACC AGTACCAGTT GTGCTACAGG 
GCAGCATTGG AGTACCTGGG GAGCTTTGAC CACTATGCAA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104931439 ( XM_019263543.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019119088.1 Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X6, mRNA.

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CloneID	OLa211350
Clone ID Related Accession (Same CDS sequence)	XM_019263543.2
Accession Version	XM_019263543.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	receptor-type tyrosine-protein phosphatase F isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040012.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_019263543.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTGGACATC 
AGCAGCAGCA ACGGGCGCAT TAAACAGCTT CGTTCAGGTG GTACACCAAT TAGAGGAGCT 
CTGCAAATTG AGAACAGCGA GGAGTCCGAC CAAGGGAAAT ACGAGTGTGT TGCCATGAAC 
AGCGCTGGGA CCCGTTACTC CGCTCCTGCC AATCTCTACG TACGAGTGCG AAGAGTCCCA 
CCCAGATTCT CCATTCCGCC CACCAACCAA GAGGTGATGC CAGGCGGCAG CGTCAACCTG 
ACATGCGTGG CAGTGGGCTC GCCGATGCCC TACGTCAAGT GGACGATGGG CCTGGTGGAG 
CTGACCAAGG AGGAGGAGAT GCCGATGGGA CGCAACGTGC TGGAGGTCAA CAACATCCGC 
GAGTCGGCGA ACTACACCTG CGTGGCCATC TCCTCTCTGG GCATGATAGA AGCCACCGCT 
CAGGTCACCG TCAAAGCTTT GCCAAAGCCT CCCGCCTCCC TCATTGTGAC TGAGACCACA 
GCCACCAGTG TGACTCTCAC CTGGGACTCT GGAAACCCAG AACCTGTCTC CTACTACATG 
ATCCAATACA GAGCCAAGAC CTCGGACAAC GGCTTCCAGG AAGTGGAAGG TGTGGCAACT 
ACCCGTTACA GCATCGGTGG ACTTAGCCCC TACTCTGAGT ATGAGTTTCG TGTCATGGCT 
GTCAACAACA TCGGCCGGGG GCCACCGAGC GACCCCGTGG AGACACGCAC TGGTGAACAG 
GCCCCCTCCT CGCCACCTCT GCACGTCCAA GCGCGCATGC TGAGTGCCAG CACAATGCTG 
GTCCAGTGGG AACCGCCCGA GGAACCCAAC GGGCAAATCA GGGGCTACCG GGTCTACTAC 
AGCTCGGACT TGACGGCTCC GCTCAGCGCC TGGCAGAAGC ACAACACAGA CGACAGCAGC 
CTGACGACCA TCTCGGGCCT GACTCCTGAT ATCACCTACA GCCTCAGGGT GCTGGGCTTC 
ACCTCTGTAG GTGACGGGCC ACCTTCTGAC ATCCTGCAAG TCAAAACCCA ACAGGGAGTC 
CCTGCCCAGC CGTCCAGCTT TGAGGCCGAA GCTGAGCTGG ACAGTCGCAT CATGCTAACA 
TGGCTGTGGC CCGTCCAGGA CCCCATCACT AAGTATGAGC TGCAGTACTG GGAGGCCGGC 
TCAGACAACA AGTTACATGT GAGCTTCAAC CCAGCGGGCT CCTACGCTGT GGACGGTCTA 
AAGCCTGACA CCTTGTACCG TTTCTCTCTG GCAGCCCGTT CTGAGATGGG CTTAGGTGTC 
TACACCCAAC CCATCGAGGC TCGCACCGCC CAGTCCACCC CATCAGCTCC CCCCCAGGAG 
GTACACCTGG TCAGCCTGAG CTCCACCAGC CTCAAGGTGA GTTGGGTGGC ACCGCCCGCC 
GCTAGCCGCC ACGGCGCCAT TGTGCGCTAC ACAGTCAGCT ACCAGGCCAT GGCTGGAGAA 
GACACGGAGC GGCACGAGGT GACGGGCATC GGCGCAGACG CCACTAGCTT TGTGCTGGAG 
GGCCTGGAGA AGTGGACTGA GTATCAGGTG TGGGTGAGGG CACACACTGA CGTGGGCCCC 
GGCCCAGAGA GTGCCCCAGT GAGGATGAGA ACCAAAGAGG ATGTGCCGGG AGCACCCCCC 
AGGAAGCTGG AGGTTGAGGC CATAAACTCC ACAGCAATCC GGGTCACCTG GAAGCCGCCC 
CTGCAGGGGA AGCAGCACGG CCAAATCCGG GGCTATCAAG TCATCTTCTC TCGGCTTGAA 
AACGGCGAAC CACGAGGCCA GCCTAACATC ATGGACGTTG CTTTGCCAGA GGCACAGTGG 
AATATTGAGG AGTCCACCGA GCATGAGGCC ATTATTGCCG GACTGCACTC TGAAACCACC 
TACTCGGTCA CCGTGGCAGC ATACACCACC AAAGGAGACG GAGCGCGCAG CAAAGCCAAA 
GTCATCACCA CGACGGGTGC AGTGCCAGGA AAGCCCACCA TGATGATCAG CACCACTATG 
GGCAACACAG CGCTGATTCA GTGGCAGCCG CCTAAGGAAA TGGTTGGGGA GCTGATGGGC 
TACCAGCTGC AGTACAAGCG CACTGACGAG GAAAACTACA CCTCCCGCGA GCTGAGAAAG 
ATCGATGACC ATTACACCGT CACCGGGCTG CACAAAGGTG CCACCTATGT CTTCCGCCTC 
AGTGCCCGCA ACCGCGCGGG CATCGGCGAA GCATACGTGA AAGAGATCAG CACCCCTGAA 
GACGTGCCGT CGGGCTTCCC GTTCAACCTG CAGGTCACGG GACTGACTCA GTCCAGCACC 
GAGCTGACCT GGGACCCTCC GCTGGTGGCC GAAAGGAACG GCAAGATCGT GCACTATGTG 
GTCGTTTACA GGGACATCAA CAGTCAGCAG AACAGCACCA ACCGGACGAG CGAAACGCAC 
ATGACCATCC AGGGCCTTAA TCCCGACACC ACCTACGATA TCCGGGTGCA GGCGTTCACC 
AGTAAGGGCG GAGGACCCCT CAGCCCTAGC ATCCAGAGCA GGACCATGTC GAACGATTTT 
CCCACCCTCA ACTTTGGTGT CAAAGCCGTC ATGAAAACAT CTGTCCTCCT CACCTGGGAT 
TTGCCACCGA ACTACAAATC CCAAGTGCCT TTCAAGATCC TGTACAACCA GCAAAGCGTG 
GAAGTGCAGG GATCCCTGAA GAGGAGGCTG ATCACGCAGC TGCAGCCAGA CACGGACTAC 
TCTTTTGTGC TGACAAGCAG GGGCAACATT GCTGGTGGTC TACAGCAGCA GGTGTCCATC 
CGCACCGCTC CCGACCTGAT CAACGCCAAG CCCAGCACAC ATCAGCCTCA GGGTAGCAAG 
ATGACAATCA ACCTGCCCAA GGTCCAGACC ACCACGCGTG TCAGGTGGTA CTACATCGTG 
GTGGTGCCGG TGTCCCAGTC AACGCAGGGG TGGAAGAATC CAGACGAGAT GGACCTGGAC 
GAGCTGTTCA AGTCAAGTGA AGGCCCCGTC CTGAGGCGCA GACGTCACCT GGATTCCTCC 
AAACCTTATA TCGCTGCCAA GTTGGCCTCG TTGCCGACCA CCTTCACCCT GGGTGATGAG 
AAGAGATACA ACGGCTTCTA CAACAAGCCC CTGAGCAGTC CGCAGGAGTA TCTCTGCTTT 
GTCCTGGCAG TACTCCGATC CGAAGGGGCA GACAGCACTA CTAATTATAT GACATTTTCA 
GCCAGTCCCT ACTCAGACCC GGTCCACGTG CGAAACAGCA TGGCTCAGGG GATGGAGCAG 
CCTGAAATGT TGTGGGTTAT GGGCCCTGTT TTGGCCGTGG TCCTGATTAT CATCATCGTC 
ATCGCCATCC TGCTCTTCAA GAACAAACAG GAAAGGAAGC GAGCGTCGCC CCTACCCAAA 
GATGAGCACT CGGGCGGGGT GAAGGACTCC CTGCTGGCCA ACTCCTCCGA CCCTGTGGAG 
ATGAGAAGAC TTAACTACCA GACCCCAGGA ATGAGGGAGC ACCCGCCCAT CCCTGTCGTC 
GACCTGGCTG ACCACATAGA GCGTCTGAAG GCCAACGACG GCCTACGCTT TTCCCAGGAG 
TACGAGTCGA TTGATCCAGG GCAGCAGTTT ACCTGGGAGA ACTCCAACAT GGAAGTCAAC 
AAGCCAAAGA ATCGCTATGC AAACGTCATC GCCTATGACC ACTCCAGAGT GGTTCTCACC 
TCTGTTGACG CCGTTCCAGG CAGTGACTAC ATCAACGCCA ACTACATCGA CGGCTACAGG 
AAGCAGAACG CCTACATCGC CACGCAGGGG CCACTTCCAG AGACACTGAG CGACTTCTGG 
AGGATGGTGT GGGAGCAAAG ATCCAACACC ATCGTCATGA TGACCAGACT GGAGGAGAAG 
TCGCGGGTGA AGTGTGACCA GTACTGGCCG TCGCGAGGAA CGGAGACCTA TGGGATGATC 
CAGGTCACCA TGCTGGACAC CGTGGAGTTG GCCACTTACA GCGTCCGTAC CTTTGCCCTC 
TATAAGAATG GCTCCAGTGA GAAGAGAGAG GTGAGACAGT TCCAGTTCAT GGCCTGGCCA 
GACCACGGGG TGCCAGAGTA CCCCACCCCA ATCCTGGCCT TCCTACGGCG GGTAAAGGCC 
TGCAATCCTC CAGACGCAGG GCCTATGGTG GTCCACTGCA GCGCGGGTGT GGGCAGGACC 
GGATGCTTCA TCGTGATAGA TGCCATGCTG GAGCGCATGA AGCACGAGAA GTCCGTAGAC 
ATCTACGGCC ACGTGACGTG CATGCGCGCC CAGAGGAACT ACATGGTCCA GACCGAGGAC 
CAGTACATCT TCATTCACGA AGCCCTGCTG GAGGCCGCCA CGTGCGGCAA CACCGAGGTG 
CCGGCGCGCA ACCTGTACGC CCACATCCAG AAACTCACCC AGCCCCCGCC CGGCGAGACG 
GTTACTGCCA TGGAGCTGGA ATTCAAGAGA CTGGCCAACT CCAAAGCCCA CACATCCCGC 
TTCATCAGCG CCAACTTGCC CTGCAACAAG TTCAAGAACC GCCTGGTCAA CATCATGCCT 
TTCGAGTCCA CCCGCGTCTG CCTGCAGCCC ATCCGTGGCG TCGAGGGCTC CGACTACATC 
AATGCCAGCT GCATCGACGG CTACAGACAG CAGAAGGCCT ACCTGGCCAC GCAGGGCCCA 
CTGGCAGAGA CCACCGAGGA CTTCTGGAGG ATGCTGTGGG AGCACAACTC CACCATCGTC 
GTCATGCTCA CCAAGCTACG AGAGATGGGC CGGGAGAAGT GCCATCAGTA CTGGCCAGCA 
GAGCGTTCAG CCAGGTACCA GTACTTTGTG GTGGATCCAA TGGCTGAGTA CAACATGCCC 
CAGTACATCC TCAGAGAGTT CAAAGTCACA GATGCCAGGG ACGGCCAGTC CAGAACAATC 
CGCCAATTCC AGTTCACTGA CTGGCCCGAG CAAGGAGTGC CAAAAACCGG AGAGGGCTTC 
ATTGATTTTA TCGGACAAGT GCACAAAACC AAGGAACAGT TCGGACAGGA CGGACCAATC 
ACTGTACACT GCAGTGCTGG GGTGGGCAGG ACCGGAGTCT TCATCACCCT CAGCATCGTG 
CTGGAGAGGA TGAGGTATGA AGGAGTTGTC GACCTCTTCC AGACAGTGAA GACACTTCGC 
ACCCAGCGGC CTGCCATGGT GCAGACCGAG GACCAGTACC AGTTGTGCTA CAGGGCAGCA 
TTGGAGTACC TGGGGAGCTT TGACCACTAT GCAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019119088.1
CDS	492..6230
Translation	MVPNIHLAVPFLPRGALLFLILLSCVVFSSLADSMPSFVKSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVEKTRTATMLCAASGNPDPEISWFKDMLPVDISSSNGRIKQLRSGGTPIRGALQIENSEESDQGKYECVAMNSAGTRYSAPANLYVRVRRVPPRFSIPPTNQEVMPGGSVNLTCVAVGSPMPYVKWTMGLVELTKEEEMPMGRNVLEVNNIRESANYTCVAISSLGMIEATAQVTVKALPKPPASLIVTETTATSVTLTWDSGNPEPVSYYMIQYRAKTSDNGFQEVEGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSDPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRVYYSSDLTAPLSAWQKHNTDDSSLTTISGLTPDITYSLRVLGFTSVGDGPPSDILQVKTQQGVPAQPSSFEAEAELDSRIMLTWLWPVQDPITKYELQYWEAGSDNKLHVSFNPAGSYAVDGLKPDTLYRFSLAARSEMGLGVYTQPIEARTAQSTPSAPPQEVHLVSLSSTSLKVSWVAPPAASRHGAIVRYTVSYQAMAGEDTERHEVTGIGADATSFVLEGLEKWTEYQVWVRAHTDVGPGPESAPVRMRTKEDVPGAPPRKLEVEAINSTAIRVTWKPPLQGKQHGQIRGYQVIFSRLENGEPRGQPNIMDVALPEAQWNIEESTEHEAIIAGLHSETTYSVTVAAYTTKGDGARSKAKVITTTGAVPGKPTMMISTTMGNTALIQWQPPKEMVGELMGYQLQYKRTDEENYTSRELRKIDDHYTVTGLHKGATYVFRLSARNRAGIGEAYVKEISTPEDVPSGFPFNLQVTGLTQSSTELTWDPPLVAERNGKIVHYVVVYRDINSQQNSTNRTSETHMTIQGLNPDTTYDIRVQAFTSKGGGPLSPSIQSRTMSNDFPTLNFGVKAVMKTSVLLTWDLPPNYKSQVPFKILYNQQSVEVQGSLKRRLITQLQPDTDYSFVLTSRGNIAGGLQQQVSIRTAPDLINAKPSTHQPQGSKMTINLPKVQTTTRVRWYYIVVVPVSQSTQGWKNPDEMDLDELFKSSEGPVLRRRRHLDSSKPYIAAKLASLPTTFTLGDEKRYNGFYNKPLSSPQEYLCFVLAVLRSEGADSTTNYMTFSASPYSDPVHVRNSMAQGMEQPEMLWVMGPVLAVVLIIIIVIAILLFKNKQERKRASPLPKDEHSGGVKDSLLANSSDPVEMRRLNYQTPGMREHPPIPVVDLADHIERLKANDGLRFSQEYESIDPGQQFTWENSNMEVNKPKNRYANVIAYDHSRVVLTSVDAVPGSDYINANYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRSNTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVTMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRAQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPPPGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYLATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_019263543.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019263543.2

ORF Insert Sequence:

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTGGACATC 
AGCAGCAGCA ACGGGCGCAT TAAACAGCTT CGTTCAGGTG GTACACCAAT TAGAGGAGCT 
CTGCAAATTG AGAACAGCGA GGAGTCCGAC CAAGGGAAAT ACGAGTGTGT TGCCATGAAC 
AGCGCTGGGA CCCGTTACTC CGCTCCTGCC AATCTCTACG TACGAGTGCG AAGAGTCCCA 
CCCAGATTCT CCATTCCGCC CACCAACCAA GAGGTGATGC CAGGCGGCAG CGTCAACCTG 
ACATGCGTGG CAGTGGGCTC GCCGATGCCC TACGTCAAGT GGACGATGGG CCTGGTGGAG 
CTGACCAAGG AGGAGGAGAT GCCGATGGGA CGCAACGTGC TGGAGGTCAA CAACATCCGC 
GAGTCGGCGA ACTACACCTG CGTGGCCATC TCCTCTCTGG GCATGATAGA AGCCACCGCT 
CAGGTCACCG TCAAAGCTTT GCCAAAGCCT CCCGCCTCCC TCATTGTGAC TGAGACCACA 
GCCACCAGTG TGACTCTCAC CTGGGACTCT GGAAACCCAG AACCTGTCTC CTACTACATG 
ATCCAATACA GAGCCAAGAC CTCGGACAAC GGCTTCCAGG AAGTGGAAGG TGTGGCAACT 
ACCCGTTACA GCATCGGTGG ACTTAGCCCC TACTCTGAGT ATGAGTTTCG TGTCATGGCT 
GTCAACAACA TCGGCCGGGG GCCACCGAGC GACCCCGTGG AGACACGCAC TGGTGAACAG 
GCCCCCTCCT CGCCACCTCT GCACGTCCAA GCGCGCATGC TGAGTGCCAG CACAATGCTG 
GTCCAGTGGG AACCGCCCGA GGAACCCAAC GGGCAAATCA GGGGCTACCG GGTCTACTAC 
AGCTCGGACT TGACGGCTCC GCTCAGCGCC TGGCAGAAGC ACAACACAGA CGACAGCAGC 
CTGACGACCA TCTCGGGCCT GACTCCTGAT ATCACCTACA GCCTCAGGGT GCTGGGCTTC 
ACCTCTGTAG GTGACGGGCC ACCTTCTGAC ATCCTGCAAG TCAAAACCCA ACAGGGAGTC 
CCTGCCCAGC CGTCCAGCTT TGAGGCCGAA GCTGAGCTGG ACAGTCGCAT CATGCTAACA 
TGGCTGTGGC CCGTCCAGGA CCCCATCACT AAGTATGAGC TGCAGTACTG GGAGGCCGGC 
TCAGACAACA AGTTACATGT GAGCTTCAAC CCAGCGGGCT CCTACGCTGT GGACGGTCTA 
AAGCCTGACA CCTTGTACCG TTTCTCTCTG GCAGCCCGTT CTGAGATGGG CTTAGGTGTC 
TACACCCAAC CCATCGAGGC TCGCACCGCC CAGTCCACCC CATCAGCTCC CCCCCAGGAG 
GTACACCTGG TCAGCCTGAG CTCCACCAGC CTCAAGGTGA GTTGGGTGGC ACCGCCCGCC 
GCTAGCCGCC ACGGCGCCAT TGTGCGCTAC ACAGTCAGCT ACCAGGCCAT GGCTGGAGAA 
GACACGGAGC GGCACGAGGT GACGGGCATC GGCGCAGACG CCACTAGCTT TGTGCTGGAG 
GGCCTGGAGA AGTGGACTGA GTATCAGGTG TGGGTGAGGG CACACACTGA CGTGGGCCCC 
GGCCCAGAGA GTGCCCCAGT GAGGATGAGA ACCAAAGAGG ATGTGCCGGG AGCACCCCCC 
AGGAAGCTGG AGGTTGAGGC CATAAACTCC ACAGCAATCC GGGTCACCTG GAAGCCGCCC 
CTGCAGGGGA AGCAGCACGG CCAAATCCGG GGCTATCAAG TCATCTTCTC TCGGCTTGAA 
AACGGCGAAC CACGAGGCCA GCCTAACATC ATGGACGTTG CTTTGCCAGA GGCACAGTGG 
AATATTGAGG AGTCCACCGA GCATGAGGCC ATTATTGCCG GACTGCACTC TGAAACCACC 
TACTCGGTCA CCGTGGCAGC ATACACCACC AAAGGAGACG GAGCGCGCAG CAAAGCCAAA 
GTCATCACCA CGACGGGTGC AGTGCCAGGA AAGCCCACCA TGATGATCAG CACCACTATG 
GGCAACACAG CGCTGATTCA GTGGCAGCCG CCTAAGGAAA TGGTTGGGGA GCTGATGGGC 
TACCAGCTGC AGTACAAGCG CACTGACGAG GAAAACTACA CCTCCCGCGA GCTGAGAAAG 
ATCGATGACC ATTACACCGT CACCGGGCTG CACAAAGGTG CCACCTATGT CTTCCGCCTC 
AGTGCCCGCA ACCGCGCGGG CATCGGCGAA GCATACGTGA AAGAGATCAG CACCCCTGAA 
GACGTGCCGT CGGGCTTCCC GTTCAACCTG CAGGTCACGG GACTGACTCA GTCCAGCACC 
GAGCTGACCT GGGACCCTCC GCTGGTGGCC GAAAGGAACG GCAAGATCGT GCACTATGTG 
GTCGTTTACA GGGACATCAA CAGTCAGCAG AACAGCACCA ACCGGACGAG CGAAACGCAC 
ATGACCATCC AGGGCCTTAA TCCCGACACC ACCTACGATA TCCGGGTGCA GGCGTTCACC 
AGTAAGGGCG GAGGACCCCT CAGCCCTAGC ATCCAGAGCA GGACCATGTC GAACGATTTT 
CCCACCCTCA ACTTTGGTGT CAAAGCCGTC ATGAAAACAT CTGTCCTCCT CACCTGGGAT 
TTGCCACCGA ACTACAAATC CCAAGTGCCT TTCAAGATCC TGTACAACCA GCAAAGCGTG 
GAAGTGCAGG GATCCCTGAA GAGGAGGCTG ATCACGCAGC TGCAGCCAGA CACGGACTAC 
TCTTTTGTGC TGACAAGCAG GGGCAACATT GCTGGTGGTC TACAGCAGCA GGTGTCCATC 
CGCACCGCTC CCGACCTGAT CAACGCCAAG CCCAGCACAC ATCAGCCTCA GGGTAGCAAG 
ATGACAATCA ACCTGCCCAA GGTCCAGACC ACCACGCGTG TCAGGTGGTA CTACATCGTG 
GTGGTGCCGG TGTCCCAGTC AACGCAGGGG TGGAAGAATC CAGACGAGAT GGACCTGGAC 
GAGCTGTTCA AGTCAAGTGA AGGCCCCGTC CTGAGGCGCA GACGTCACCT GGATTCCTCC 
AAACCTTATA TCGCTGCCAA GTTGGCCTCG TTGCCGACCA CCTTCACCCT GGGTGATGAG 
AAGAGATACA ACGGCTTCTA CAACAAGCCC CTGAGCAGTC CGCAGGAGTA TCTCTGCTTT 
GTCCTGGCAG TACTCCGATC CGAAGGGGCA GACAGCACTA CTAATTATAT GACATTTTCA 
GCCAGTCCCT ACTCAGACCC GGTCCACGTG CGAAACAGCA TGGCTCAGGG GATGGAGCAG 
CCTGAAATGT TGTGGGTTAT GGGCCCTGTT TTGGCCGTGG TCCTGATTAT CATCATCGTC 
ATCGCCATCC TGCTCTTCAA GAACAAACAG GAAAGGAAGC GAGCGTCGCC CCTACCCAAA 
GATGAGCACT CGGGCGGGGT GAAGGACTCC CTGCTGGCCA ACTCCTCCGA CCCTGTGGAG 
ATGAGAAGAC TTAACTACCA GACCCCAGGA ATGAGGGAGC ACCCGCCCAT CCCTGTCGTC 
GACCTGGCTG ACCACATAGA GCGTCTGAAG GCCAACGACG GCCTACGCTT TTCCCAGGAG 
TACGAGTCGA TTGATCCAGG GCAGCAGTTT ACCTGGGAGA ACTCCAACAT GGAAGTCAAC 
AAGCCAAAGA ATCGCTATGC AAACGTCATC GCCTATGACC ACTCCAGAGT GGTTCTCACC 
TCTGTTGACG CCGTTCCAGG CAGTGACTAC ATCAACGCCA ACTACATCGA CGGCTACAGG 
AAGCAGAACG CCTACATCGC CACGCAGGGG CCACTTCCAG AGACACTGAG CGACTTCTGG 
AGGATGGTGT GGGAGCAAAG ATCCAACACC ATCGTCATGA TGACCAGACT GGAGGAGAAG 
TCGCGGGTGA AGTGTGACCA GTACTGGCCG TCGCGAGGAA CGGAGACCTA TGGGATGATC 
CAGGTCACCA TGCTGGACAC CGTGGAGTTG GCCACTTACA GCGTCCGTAC CTTTGCCCTC 
TATAAGAATG GCTCCAGTGA GAAGAGAGAG GTGAGACAGT TCCAGTTCAT GGCCTGGCCA 
GACCACGGGG TGCCAGAGTA CCCCACCCCA ATCCTGGCCT TCCTACGGCG GGTAAAGGCC 
TGCAATCCTC CAGACGCAGG GCCTATGGTG GTCCACTGCA GCGCGGGTGT GGGCAGGACC 
GGATGCTTCA TCGTGATAGA TGCCATGCTG GAGCGCATGA AGCACGAGAA GTCCGTAGAC 
ATCTACGGCC ACGTGACGTG CATGCGCGCC CAGAGGAACT ACATGGTCCA GACCGAGGAC 
CAGTACATCT TCATTCACGA AGCCCTGCTG GAGGCCGCCA CGTGCGGCAA CACCGAGGTG 
CCGGCGCGCA ACCTGTACGC CCACATCCAG AAACTCACCC AGCCCCCGCC CGGCGAGACG 
GTTACTGCCA TGGAGCTGGA ATTCAAGAGA CTGGCCAACT CCAAAGCCCA CACATCCCGC 
TTCATCAGCG CCAACTTGCC CTGCAACAAG TTCAAGAACC GCCTGGTCAA CATCATGCCT 
TTCGAGTCCA CCCGCGTCTG CCTGCAGCCC ATCCGTGGCG TCGAGGGCTC CGACTACATC 
AATGCCAGCT GCATCGACGG CTACAGACAG CAGAAGGCCT ACCTGGCCAC GCAGGGCCCA 
CTGGCAGAGA CCACCGAGGA CTTCTGGAGG ATGCTGTGGG AGCACAACTC CACCATCGTC 
GTCATGCTCA CCAAGCTACG AGAGATGGGC CGGGAGAAGT GCCATCAGTA CTGGCCAGCA 
GAGCGTTCAG CCAGGTACCA GTACTTTGTG GTGGATCCAA TGGCTGAGTA CAACATGCCC 
CAGTACATCC TCAGAGAGTT CAAAGTCACA GATGCCAGGG ACGGCCAGTC CAGAACAATC 
CGCCAATTCC AGTTCACTGA CTGGCCCGAG CAAGGAGTGC CAAAAACCGG AGAGGGCTTC 
ATTGATTTTA TCGGACAAGT GCACAAAACC AAGGAACAGT TCGGACAGGA CGGACCAATC 
ACTGTACACT GCAGTGCTGG GGTGGGCAGG ACCGGAGTCT TCATCACCCT CAGCATCGTG 
CTGGAGAGGA TGAGGTATGA AGGAGTTGTC GACCTCTTCC AGACAGTGAA GACACTTCGC 
ACCCAGCGGC CTGCCATGGT GCAGACCGAG GACCAGTACC AGTTGTGCTA CAGGGCAGCA 
TTGGAGTACC TGGGGAGCTT TGACCACTAT GCAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104931439 ( XM_010746447.3 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010744749.2 Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X7, mRNA.

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CloneID	OLa211351
Clone ID Related Accession (Same CDS sequence)	XM_010746447.3
Accession Version	XM_010746447.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	receptor-type tyrosine-protein phosphatase F isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040012.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_010746447.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTGGACATC 
AGCAGCAGCA ACGGGCGCAT TAAACAGCTT CGTTCAGGAG CTCTGCAAAT TGAGAACAGC 
GAGGAGTCCG ACCAAGGGAA ATACGAGTGT GTTGCCATGA ACAGCGCTGG GACCCGTTAC 
TCCGCTCCTG CCAATCTCTA CGTACGAGTG CGAAGAGTCC CACCCAGATT CTCCATTCCG 
CCCACCAACC AAGAGGTGAT GCCAGGCGGC AGCGTCAACC TGACATGCGT GGCAGTGGGC 
TCGCCGATGC CCTACGTCAA GTGGACGATG GGCCTGGTGG AGCTGACCAA GGAGGAGGAG 
ATGCCGATGG GACGCAACGT GCTGGAGGTC AACAACATCC GCGAGTCGGC GAACTACACC 
TGCGTGGCCA TCTCCTCTCT GGGCATGATA GAAGCCACCG CTCAGGTCAC CGTCAAAGCT 
TTGCCAAAGC CTCCCGCCTC CCTCATTGTG ACTGAGACCA CAGCCACCAG TGTGACTCTC 
ACCTGGGACT CTGGAAACCC AGAACCTGTC TCCTACTACA TGATCCAATA CAGAGCCAAG 
ACCTCGGACA ACGGCTTCCA GGAAGTGGAA GGTGTGGCAA CTACCCGTTA CAGCATCGGT 
GGACTTAGCC CCTACTCTGA GTATGAGTTT CGTGTCATGG CTGTCAACAA CATCGGCCGG 
GGGCCACCGA GCGACCCCGT GGAGACACGC ACTGGTGAAC AGGCCCCCTC CTCGCCACCT 
CTGCACGTCC AAGCGCGCAT GCTGAGTGCC AGCACAATGC TGGTCCAGTG GGAACCGCCC 
GAGGAACCCA ACGGGCAAAT CAGGGGCTAC CGGGTCTACT ACAGCTCGGA CTTGACGGCT 
CCGCTCAGCG CCTGGCAGAA GCACAACACA GACGACAGCA GCCTGACGAC CATCTCGGGC 
CTGACTCCTG ATATCACCTA CAGCCTCAGG GTGCTGGGCT TCACCTCTGT AGGTGACGGG 
CCACCTTCTG ACATCCTGCA AGTCAAAACC CAACAGGGAG TCCCTGCCCA GCCGTCCAGC 
TTTGAGGCCG AAGCTGAGCT GGACAGTCGC ATCATGCTAA CATGGCTGTG GCCCGTCCAG 
GACCCCATCA CTAAGTATGA GCTGCAGTAC TGGGAGGCCG GCTCAGACAA CAAGTTACAT 
GTGAGCTTCA ACCCAGCGGG CTCCTACGCT GTGGACGGTC TAAAGCCTGA CACCTTGTAC 
CGTTTCTCTC TGGCAGCCCG TTCTGAGATG GGCTTAGGTG TCTACACCCA ACCCATCGAG 
GCTCGCACCG CCCAGTCCAC CCCATCAGCT CCCCCCCAGG AGGTACACCT GGTCAGCCTG 
AGCTCCACCA GCCTCAAGGT GAGTTGGGTG GCACCGCCCG CCGCTAGCCG CCACGGCGCC 
ATTGTGCGCT ACACAGTCAG CTACCAGGCC ATGGCTGGAG AAGACACGGA GCGGCACGAG 
GTGACGGGCA TCGGCGCAGA CGCCACTAGC TTTGTGCTGG AGGGCCTGGA GAAGTGGACT 
GAGTATCAGG TGTGGGTGAG GGCACACACT GACGTGGGCC CCGGCCCAGA GAGTGCCCCA 
GTGAGGATGA GAACCAAAGA GGATGTGCCG GGAGCACCCC CCAGGAAGCT GGAGGTTGAG 
GCCATAAACT CCACAGCAAT CCGGGTCACC TGGAAGCCGC CCCTGCAGGG GAAGCAGCAC 
GGCCAAATCC GGGGCTATCA AGTCATCTTC TCTCGGCTTG AAAACGGCGA ACCACGAGGC 
CAGCCTAACA TCATGGACGT TGCTTTGCCA GAGGCACAGG AGGCCATTAT TGCCGGACTG 
CACTCTGAAA CCACCTACTC GGTCACCGTG GCAGCATACA CCACCAAAGG AGACGGAGCG 
CGCAGCAAAG CCAAAGTCAT CACCACGACG GGTGCAGTGC CAGGAAAGCC CACCATGATG 
ATCAGCACCA CTATGGGCAA CACAGCGCTG ATTCAGTGGC AGCCGCCTAA GGAAATGGTT 
GGGGAGCTGA TGGGCTACCA GCTGCAGTAC AAGCGCACTG ACGAGGAAAA CTACACCTCC 
CGCGAGCTGA GAAAGATCGA TGACCATTAC ACCGTCACCG GGCTGCACAA AGGTGCCACC 
TATGTCTTCC GCCTCAGTGC CCGCAACCGC GCGGGCATCG GCGAAGCATA CGTGAAAGAG 
ATCAGCACCC CTGAAGACGT GCCGTCGGGC TTCCCGTTCA ACCTGCAGGT CACGGGACTG 
ACTCAGTCCA GCACCGAGCT GACCTGGGAC CCTCCGCTGG TGGCCGAAAG GAACGGCAAG 
ATCGTGCACT ATGTGGTCGT TTACAGGGAC ATCAACAGTC AGCAGAACAG CACCAACCGG 
ACGAGCGAAA CGCACATGAC CATCCAGGGC CTTAATCCCG ACACCACCTA CGATATCCGG 
GTGCAGGCGT TCACCAGTAA GGGCGGAGGA CCCCTCAGCC CTAGCATCCA GAGCAGGACC 
ATGTCGAACG ATTTTCCCAC CCTCAACTTT GGTGTCAAAG CCGTCATGAA AACATCTGTC 
CTCCTCACCT GGGATTTGCC ACCGAACTAC AAATCCCAAG TGCCTTTCAA GATCCTGTAC 
AACCAGCAAA GCGTGGAAGT GCAGGGATCC CTGAAGAGGA GGCTGATCAC GCAGCTGCAG 
CCAGACACGG ACTACTCTTT TGTGCTGACA AGCAGGGGCA ACATTGCTGG TGGTCTACAG 
CAGCAGGTGT CCATCCGCAC CGCTCCCGAC CTGATCAACG CCAAGCCCAG CACACATCAG 
CCTCAGGGTA GCAAGATGAC AATCAACCTG CCCAAGGTCC AGACCACCAC GCGTGTCAGG 
TGGTACTACA TCGTGGTGGT GCCGGTGTCC CAGTCAACGC AGGGGTGGAA GAATCCAGAC 
GAGATGGACC TGGACGAGCT GTTCAAGTCA AGTGAAGGCC CCGTCCTGAG GCGCAGACGT 
CACCTGGATT CCTCCAAACC TTATATCGCT GCCAAGTTGG CCTCGTTGCC GACCACCTTC 
ACCCTGGGTG ATGAGAAGAG ATACAACGGC TTCTACAACA AGCCCCTGAG CAGTCCGCAG 
GAGTATCTCT GCTTTGTCCT GGCAGTACTC CGATCCGAAG GGGCAGACAG CACTACTAAT 
TATATGACAT TTTCAGCCAG TCCCTACTCA GACCCGGTCC ACGTGCGAAA CAGCATGGCT 
CAGGGGATGG AGCAGCCTGA AATGTTGTGG GTTATGGGCC CTGTTTTGGC CGTGGTCCTG 
ATTATCATCA TCGTCATCGC CATCCTGCTC TTCAAGAACA AACAGGAAAG GAAGCGAGCG 
TCGCCCCTAC CCAAAGATGA GCACTCGGGC GGGGTGAAGG ACTCCCTGCT GGCCAACTCC 
TCCGACCCTG TGGAGATGAG AAGACTTAAC TACCAGACCC CAGGTCCCAG CTCTCATCGC 
TGCCCCAACA CTCCAAGAAT GAGGGAGCAC CCGCCCATCC CTGTCGTCGA CCTGGCTGAC 
CACATAGAGC GTCTGAAGGC CAACGACGGC CTACGCTTTT CCCAGGAGTA CGAGTCGATT 
GATCCAGGGC AGCAGTTTAC CTGGGAGAAC TCCAACATGG AAGTCAACAA GCCAAAGAAT 
CGCTATGCAA ACGTCATCGC CTATGACCAC TCCAGAGTGG TTCTCACCTC TGTTGACGCC 
GTTCCAGGCA GTGACTACAT CAACGCCAAC TACATCGACG GCTACAGGAA GCAGAACGCC 
TACATCGCCA CGCAGGGGCC ACTTCCAGAG ACACTGAGCG ACTTCTGGAG GATGGTGTGG 
GAGCAAAGAT CCAACACCAT CGTCATGATG ACCAGACTGG AGGAGAAGTC GCGGGTGAAG 
TGTGACCAGT ACTGGCCGTC GCGAGGAACG GAGACCTATG GGATGATCCA GGTCACCATG 
CTGGACACCG TGGAGTTGGC CACTTACAGC GTCCGTACCT TTGCCCTCTA TAAGAATGGC 
TCCAGTGAGA AGAGAGAGGT GAGACAGTTC CAGTTCATGG CCTGGCCAGA CCACGGGGTG 
CCAGAGTACC CCACCCCAAT CCTGGCCTTC CTACGGCGGG TAAAGGCCTG CAATCCTCCA 
GACGCAGGGC CTATGGTGGT CCACTGCAGC GCGGGTGTGG GCAGGACCGG ATGCTTCATC 
GTGATAGATG CCATGCTGGA GCGCATGAAG CACGAGAAGT CCGTAGACAT CTACGGCCAC 
GTGACGTGCA TGCGCGCCCA GAGGAACTAC ATGGTCCAGA CCGAGGACCA GTACATCTTC 
ATTCACGAAG CCCTGCTGGA GGCCGCCACG TGCGGCAACA CCGAGGTGCC GGCGCGCAAC 
CTGTACGCCC ACATCCAGAA ACTCACCCAG CCCCCGCCCG GCGAGACGGT TACTGCCATG 
GAGCTGGAAT TCAAGAGACT GGCCAACTCC AAAGCCCACA CATCCCGCTT CATCAGCGCC 
AACTTGCCCT GCAACAAGTT CAAGAACCGC CTGGTCAACA TCATGCCTTT CGAGTCCACC 
CGCGTCTGCC TGCAGCCCAT CCGTGGCGTC GAGGGCTCCG ACTACATCAA TGCCAGCTGC 
ATCGACGGCT ACAGACAGCA GAAGGCCTAC CTGGCCACGC AGGGCCCACT GGCAGAGACC 
ACCGAGGACT TCTGGAGGAT GCTGTGGGAG CACAACTCCA CCATCGTCGT CATGCTCACC 
AAGCTACGAG AGATGGGCCG GGAGAAGTGC CATCAGTACT GGCCAGCAGA GCGTTCAGCC 
AGGTACCAGT ACTTTGTGGT GGATCCAATG GCTGAGTACA ACATGCCCCA GTACATCCTC 
AGAGAGTTCA AAGTCACAGA TGCCAGGGAC GGCCAGTCCA GAACAATCCG CCAATTCCAG 
TTCACTGACT GGCCCGAGCA AGGAGTGCCA AAAACCGGAG AGGGCTTCAT TGATTTTATC 
GGACAAGTGC ACAAAACCAA GGAACAGTTC GGACAGGACG GACCAATCAC TGTACACTGC 
AGTGCTGGGG TGGGCAGGAC CGGAGTCTTC ATCACCCTCA GCATCGTGCT GGAGAGGATG 
AGGTATGAAG GAGTTGTCGA CCTCTTCCAG ACAGTGAAGA CACTTCGCAC CCAGCGGCCT 
GCCATGGTGC AGACCGAGGA CCAGTACCAG TTGTGCTACA GGGCAGCATT GGAGTACCTG 
GGGAGCTTTG ACCACTATGC AACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010744749.2
CDS	485..6211
Translation	MVPNIHLAVPFLPRGALLFLILLSCVVFSSLADSMPSFVKSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVEKTRTATMLCAASGNPDPEISWFKDMLPVDISSSNGRIKQLRSGALQIENSEESDQGKYECVAMNSAGTRYSAPANLYVRVRRVPPRFSIPPTNQEVMPGGSVNLTCVAVGSPMPYVKWTMGLVELTKEEEMPMGRNVLEVNNIRESANYTCVAISSLGMIEATAQVTVKALPKPPASLIVTETTATSVTLTWDSGNPEPVSYYMIQYRAKTSDNGFQEVEGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSDPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRVYYSSDLTAPLSAWQKHNTDDSSLTTISGLTPDITYSLRVLGFTSVGDGPPSDILQVKTQQGVPAQPSSFEAEAELDSRIMLTWLWPVQDPITKYELQYWEAGSDNKLHVSFNPAGSYAVDGLKPDTLYRFSLAARSEMGLGVYTQPIEARTAQSTPSAPPQEVHLVSLSSTSLKVSWVAPPAASRHGAIVRYTVSYQAMAGEDTERHEVTGIGADATSFVLEGLEKWTEYQVWVRAHTDVGPGPESAPVRMRTKEDVPGAPPRKLEVEAINSTAIRVTWKPPLQGKQHGQIRGYQVIFSRLENGEPRGQPNIMDVALPEAQEAIIAGLHSETTYSVTVAAYTTKGDGARSKAKVITTTGAVPGKPTMMISTTMGNTALIQWQPPKEMVGELMGYQLQYKRTDEENYTSRELRKIDDHYTVTGLHKGATYVFRLSARNRAGIGEAYVKEISTPEDVPSGFPFNLQVTGLTQSSTELTWDPPLVAERNGKIVHYVVVYRDINSQQNSTNRTSETHMTIQGLNPDTTYDIRVQAFTSKGGGPLSPSIQSRTMSNDFPTLNFGVKAVMKTSVLLTWDLPPNYKSQVPFKILYNQQSVEVQGSLKRRLITQLQPDTDYSFVLTSRGNIAGGLQQQVSIRTAPDLINAKPSTHQPQGSKMTINLPKVQTTTRVRWYYIVVVPVSQSTQGWKNPDEMDLDELFKSSEGPVLRRRRHLDSSKPYIAAKLASLPTTFTLGDEKRYNGFYNKPLSSPQEYLCFVLAVLRSEGADSTTNYMTFSASPYSDPVHVRNSMAQGMEQPEMLWVMGPVLAVVLIIIIVIAILLFKNKQERKRASPLPKDEHSGGVKDSLLANSSDPVEMRRLNYQTPGPSSHRCPNTPRMREHPPIPVVDLADHIERLKANDGLRFSQEYESIDPGQQFTWENSNMEVNKPKNRYANVIAYDHSRVVLTSVDAVPGSDYINANYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRSNTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVTMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRAQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPPPGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYLATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_010746447.3
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010746447.3

ORF Insert Sequence:

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTGGACATC 
AGCAGCAGCA ACGGGCGCAT TAAACAGCTT CGTTCAGGAG CTCTGCAAAT TGAGAACAGC 
GAGGAGTCCG ACCAAGGGAA ATACGAGTGT GTTGCCATGA ACAGCGCTGG GACCCGTTAC 
TCCGCTCCTG CCAATCTCTA CGTACGAGTG CGAAGAGTCC CACCCAGATT CTCCATTCCG 
CCCACCAACC AAGAGGTGAT GCCAGGCGGC AGCGTCAACC TGACATGCGT GGCAGTGGGC 
TCGCCGATGC CCTACGTCAA GTGGACGATG GGCCTGGTGG AGCTGACCAA GGAGGAGGAG 
ATGCCGATGG GACGCAACGT GCTGGAGGTC AACAACATCC GCGAGTCGGC GAACTACACC 
TGCGTGGCCA TCTCCTCTCT GGGCATGATA GAAGCCACCG CTCAGGTCAC CGTCAAAGCT 
TTGCCAAAGC CTCCCGCCTC CCTCATTGTG ACTGAGACCA CAGCCACCAG TGTGACTCTC 
ACCTGGGACT CTGGAAACCC AGAACCTGTC TCCTACTACA TGATCCAATA CAGAGCCAAG 
ACCTCGGACA ACGGCTTCCA GGAAGTGGAA GGTGTGGCAA CTACCCGTTA CAGCATCGGT 
GGACTTAGCC CCTACTCTGA GTATGAGTTT CGTGTCATGG CTGTCAACAA CATCGGCCGG 
GGGCCACCGA GCGACCCCGT GGAGACACGC ACTGGTGAAC AGGCCCCCTC CTCGCCACCT 
CTGCACGTCC AAGCGCGCAT GCTGAGTGCC AGCACAATGC TGGTCCAGTG GGAACCGCCC 
GAGGAACCCA ACGGGCAAAT CAGGGGCTAC CGGGTCTACT ACAGCTCGGA CTTGACGGCT 
CCGCTCAGCG CCTGGCAGAA GCACAACACA GACGACAGCA GCCTGACGAC CATCTCGGGC 
CTGACTCCTG ATATCACCTA CAGCCTCAGG GTGCTGGGCT TCACCTCTGT AGGTGACGGG 
CCACCTTCTG ACATCCTGCA AGTCAAAACC CAACAGGGAG TCCCTGCCCA GCCGTCCAGC 
TTTGAGGCCG AAGCTGAGCT GGACAGTCGC ATCATGCTAA CATGGCTGTG GCCCGTCCAG 
GACCCCATCA CTAAGTATGA GCTGCAGTAC TGGGAGGCCG GCTCAGACAA CAAGTTACAT 
GTGAGCTTCA ACCCAGCGGG CTCCTACGCT GTGGACGGTC TAAAGCCTGA CACCTTGTAC 
CGTTTCTCTC TGGCAGCCCG TTCTGAGATG GGCTTAGGTG TCTACACCCA ACCCATCGAG 
GCTCGCACCG CCCAGTCCAC CCCATCAGCT CCCCCCCAGG AGGTACACCT GGTCAGCCTG 
AGCTCCACCA GCCTCAAGGT GAGTTGGGTG GCACCGCCCG CCGCTAGCCG CCACGGCGCC 
ATTGTGCGCT ACACAGTCAG CTACCAGGCC ATGGCTGGAG AAGACACGGA GCGGCACGAG 
GTGACGGGCA TCGGCGCAGA CGCCACTAGC TTTGTGCTGG AGGGCCTGGA GAAGTGGACT 
GAGTATCAGG TGTGGGTGAG GGCACACACT GACGTGGGCC CCGGCCCAGA GAGTGCCCCA 
GTGAGGATGA GAACCAAAGA GGATGTGCCG GGAGCACCCC CCAGGAAGCT GGAGGTTGAG 
GCCATAAACT CCACAGCAAT CCGGGTCACC TGGAAGCCGC CCCTGCAGGG GAAGCAGCAC 
GGCCAAATCC GGGGCTATCA AGTCATCTTC TCTCGGCTTG AAAACGGCGA ACCACGAGGC 
CAGCCTAACA TCATGGACGT TGCTTTGCCA GAGGCACAGG AGGCCATTAT TGCCGGACTG 
CACTCTGAAA CCACCTACTC GGTCACCGTG GCAGCATACA CCACCAAAGG AGACGGAGCG 
CGCAGCAAAG CCAAAGTCAT CACCACGACG GGTGCAGTGC CAGGAAAGCC CACCATGATG 
ATCAGCACCA CTATGGGCAA CACAGCGCTG ATTCAGTGGC AGCCGCCTAA GGAAATGGTT 
GGGGAGCTGA TGGGCTACCA GCTGCAGTAC AAGCGCACTG ACGAGGAAAA CTACACCTCC 
CGCGAGCTGA GAAAGATCGA TGACCATTAC ACCGTCACCG GGCTGCACAA AGGTGCCACC 
TATGTCTTCC GCCTCAGTGC CCGCAACCGC GCGGGCATCG GCGAAGCATA CGTGAAAGAG 
ATCAGCACCC CTGAAGACGT GCCGTCGGGC TTCCCGTTCA ACCTGCAGGT CACGGGACTG 
ACTCAGTCCA GCACCGAGCT GACCTGGGAC CCTCCGCTGG TGGCCGAAAG GAACGGCAAG 
ATCGTGCACT ATGTGGTCGT TTACAGGGAC ATCAACAGTC AGCAGAACAG CACCAACCGG 
ACGAGCGAAA CGCACATGAC CATCCAGGGC CTTAATCCCG ACACCACCTA CGATATCCGG 
GTGCAGGCGT TCACCAGTAA GGGCGGAGGA CCCCTCAGCC CTAGCATCCA GAGCAGGACC 
ATGTCGAACG ATTTTCCCAC CCTCAACTTT GGTGTCAAAG CCGTCATGAA AACATCTGTC 
CTCCTCACCT GGGATTTGCC ACCGAACTAC AAATCCCAAG TGCCTTTCAA GATCCTGTAC 
AACCAGCAAA GCGTGGAAGT GCAGGGATCC CTGAAGAGGA GGCTGATCAC GCAGCTGCAG 
CCAGACACGG ACTACTCTTT TGTGCTGACA AGCAGGGGCA ACATTGCTGG TGGTCTACAG 
CAGCAGGTGT CCATCCGCAC CGCTCCCGAC CTGATCAACG CCAAGCCCAG CACACATCAG 
CCTCAGGGTA GCAAGATGAC AATCAACCTG CCCAAGGTCC AGACCACCAC GCGTGTCAGG 
TGGTACTACA TCGTGGTGGT GCCGGTGTCC CAGTCAACGC AGGGGTGGAA GAATCCAGAC 
GAGATGGACC TGGACGAGCT GTTCAAGTCA AGTGAAGGCC CCGTCCTGAG GCGCAGACGT 
CACCTGGATT CCTCCAAACC TTATATCGCT GCCAAGTTGG CCTCGTTGCC GACCACCTTC 
ACCCTGGGTG ATGAGAAGAG ATACAACGGC TTCTACAACA AGCCCCTGAG CAGTCCGCAG 
GAGTATCTCT GCTTTGTCCT GGCAGTACTC CGATCCGAAG GGGCAGACAG CACTACTAAT 
TATATGACAT TTTCAGCCAG TCCCTACTCA GACCCGGTCC ACGTGCGAAA CAGCATGGCT 
CAGGGGATGG AGCAGCCTGA AATGTTGTGG GTTATGGGCC CTGTTTTGGC CGTGGTCCTG 
ATTATCATCA TCGTCATCGC CATCCTGCTC TTCAAGAACA AACAGGAAAG GAAGCGAGCG 
TCGCCCCTAC CCAAAGATGA GCACTCGGGC GGGGTGAAGG ACTCCCTGCT GGCCAACTCC 
TCCGACCCTG TGGAGATGAG AAGACTTAAC TACCAGACCC CAGGTCCCAG CTCTCATCGC 
TGCCCCAACA CTCCAAGAAT GAGGGAGCAC CCGCCCATCC CTGTCGTCGA CCTGGCTGAC 
CACATAGAGC GTCTGAAGGC CAACGACGGC CTACGCTTTT CCCAGGAGTA CGAGTCGATT 
GATCCAGGGC AGCAGTTTAC CTGGGAGAAC TCCAACATGG AAGTCAACAA GCCAAAGAAT 
CGCTATGCAA ACGTCATCGC CTATGACCAC TCCAGAGTGG TTCTCACCTC TGTTGACGCC 
GTTCCAGGCA GTGACTACAT CAACGCCAAC TACATCGACG GCTACAGGAA GCAGAACGCC 
TACATCGCCA CGCAGGGGCC ACTTCCAGAG ACACTGAGCG ACTTCTGGAG GATGGTGTGG 
GAGCAAAGAT CCAACACCAT CGTCATGATG ACCAGACTGG AGGAGAAGTC GCGGGTGAAG 
TGTGACCAGT ACTGGCCGTC GCGAGGAACG GAGACCTATG GGATGATCCA GGTCACCATG 
CTGGACACCG TGGAGTTGGC CACTTACAGC GTCCGTACCT TTGCCCTCTA TAAGAATGGC 
TCCAGTGAGA AGAGAGAGGT GAGACAGTTC CAGTTCATGG CCTGGCCAGA CCACGGGGTG 
CCAGAGTACC CCACCCCAAT CCTGGCCTTC CTACGGCGGG TAAAGGCCTG CAATCCTCCA 
GACGCAGGGC CTATGGTGGT CCACTGCAGC GCGGGTGTGG GCAGGACCGG ATGCTTCATC 
GTGATAGATG CCATGCTGGA GCGCATGAAG CACGAGAAGT CCGTAGACAT CTACGGCCAC 
GTGACGTGCA TGCGCGCCCA GAGGAACTAC ATGGTCCAGA CCGAGGACCA GTACATCTTC 
ATTCACGAAG CCCTGCTGGA GGCCGCCACG TGCGGCAACA CCGAGGTGCC GGCGCGCAAC 
CTGTACGCCC ACATCCAGAA ACTCACCCAG CCCCCGCCCG GCGAGACGGT TACTGCCATG 
GAGCTGGAAT TCAAGAGACT GGCCAACTCC AAAGCCCACA CATCCCGCTT CATCAGCGCC 
AACTTGCCCT GCAACAAGTT CAAGAACCGC CTGGTCAACA TCATGCCTTT CGAGTCCACC 
CGCGTCTGCC TGCAGCCCAT CCGTGGCGTC GAGGGCTCCG ACTACATCAA TGCCAGCTGC 
ATCGACGGCT ACAGACAGCA GAAGGCCTAC CTGGCCACGC AGGGCCCACT GGCAGAGACC 
ACCGAGGACT TCTGGAGGAT GCTGTGGGAG CACAACTCCA CCATCGTCGT CATGCTCACC 
AAGCTACGAG AGATGGGCCG GGAGAAGTGC CATCAGTACT GGCCAGCAGA GCGTTCAGCC 
AGGTACCAGT ACTTTGTGGT GGATCCAATG GCTGAGTACA ACATGCCCCA GTACATCCTC 
AGAGAGTTCA AAGTCACAGA TGCCAGGGAC GGCCAGTCCA GAACAATCCG CCAATTCCAG 
TTCACTGACT GGCCCGAGCA AGGAGTGCCA AAAACCGGAG AGGGCTTCAT TGATTTTATC 
GGACAAGTGC ACAAAACCAA GGAACAGTTC GGACAGGACG GACCAATCAC TGTACACTGC 
AGTGCTGGGG TGGGCAGGAC CGGAGTCTTC ATCACCCTCA GCATCGTGCT GGAGAGGATG 
AGGTATGAAG GAGTTGTCGA CCTCTTCCAG ACAGTGAAGA CACTTCGCAC CCAGCGGCCT 
GCCATGGTGC AGACCGAGGA CCAGTACCAG TTGTGCTACA GGGCAGCATT GGAGTACCTG 
GGGAGCTTTG ACCACTATGC AACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104931439 ( XM_019263557.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019119102.1 Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X8, mRNA.

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CloneID	OLa211352
Clone ID Related Accession (Same CDS sequence)	XM_019263557.2
Accession Version	XM_019263557.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	receptor-type tyrosine-protein phosphatase F isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040012.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_019263557.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTGGACATC 
AGCAGCAGCA ACGGGCGCAT TAAACAGCTT CGTTCAGGTG GTACACCAAT TAGAGGAGCT 
CTGCAAATTG AGAACAGCGA GGAGTCCGAC CAAGGGAAAT ACGAGTGTGT TGCCATGAAC 
AGCGCTGGGA CCCGTTACTC CGCTCCTGCC AATCTCTACG TACGAGTGCG AAGAGTCCCA 
CCCAGATTCT CCATTCCGCC CACCAACCAA GAGGTGATGC CAGGCGGCAG CGTCAACCTG 
ACATGCGTGG CAGTGGGCTC GCCGATGCCC TACGTCAAGT GGACGATGGG CCTGGTGGAG 
CTGACCAAGG AGGAGGAGAT GCCGATGGGA CGCAACGTGC TGGAGGTCAA CAACATCCGC 
GAGTCGGCGA ACTACACCTG CGTGGCCATC TCCTCTCTGG GCATGATAGA AGCCACCGCT 
CAGGTCACCG TCAAAGCTTT GCCAAAGCCT CCCGCCTCCC TCATTGTGAC TGAGACCACA 
GCCACCAGTG TGACTCTCAC CTGGGACTCT GGAAACCCAG AACCTGTCTC CTACTACATG 
ATCCAATACA GAGCCAAGAC CTCGGACAAC GGCTTCCAGG AAGTGGAAGG TGTGGCAACT 
ACCCGTTACA GCATCGGTGG ACTTAGCCCC TACTCTGAGT ATGAGTTTCG TGTCATGGCT 
GTCAACAACA TCGGCCGGGG GCCACCGAGC GACCCCGTGG AGACACGCAC TGGTGAACAG 
GCCCCCTCCT CGCCACCTCT GCACGTCCAA GCGCGCATGC TGAGTGCCAG CACAATGCTG 
GTCCAGTGGG AACCGCCCGA GGAACCCAAC GGGCAAATCA GGGGCTACCG GGTCTACTAC 
AGCTCGGACT TGACGGCTCC GCTCAGCGCC TGGCAGAAGC ACAACACAGA CGACAGCAGC 
CTGACGACCA TCTCGGGCCT GACTCCTGAT ATCACCTACA GCCTCAGGGT GCTGGGCTTC 
ACCTCTGTAG GTGACGGGCC ACCTTCTGAC ATCCTGCAAG TCAAAACCCA ACAGGGAGTC 
CCTGCCCAGC CGTCCAGCTT TGAGGCCGAA GCTGAGCTGG ACAGTCGCAT CATGCTAACA 
TGGCTGTGGC CCGTCCAGGA CCCCATCACT AAGTATGAGC TGCAGTACTG GGAGGCCGGC 
TCAGACAACA AGTTACATGT GAGCTTCAAC CCAGCGGGCT CCTACGCTGT GGACGGTCTA 
AAGCCTGACA CCTTGTACCG TTTCTCTCTG GCAGCCCGTT CTGAGATGGG CTTAGGTGTC 
TACACCCAAC CCATCGAGGC TCGCACCGCC CAGTCCACCC CATCAGCTCC CCCCCAGGAG 
GTACACCTGG TCAGCCTGAG CTCCACCAGC CTCAAGGTGA GTTGGGTGGC ACCGCCCGCC 
GCTAGCCGCC ACGGCGCCAT TGTGCGCTAC ACAGTCAGCT ACCAGGCCAT GGCTGGAGAA 
GACACGGAGC GGCACGAGGT GACGGGCATC GGCGCAGACG CCACTAGCTT TGTGCTGGAG 
GGCCTGGAGA AGTGGACTGA GTATCAGGTG TGGGTGAGGG CACACACTGA CGTGGGCCCC 
GGCCCAGAGA GTGCCCCAGT GAGGATGAGA ACCAAAGAGG ATGTGCCGGG AGCACCCCCC 
AGGAAGCTGG AGGTTGAGGC CATAAACTCC ACAGCAATCC GGGTCACCTG GAAGCCGCCC 
CTGCAGGGGA AGCAGCACGG CCAAATCCGG GGCTATCAAG TCATCTTCTC TCGGCTTGAA 
AACGGCGAAC CACGAGGCCA GCCTAACATC ATGGACGTTG CTTTGCCAGA GGCACAGTGG 
AATATTGAGG AGTCCACCGA GCATGAGGCC ATTATTGCCG GACTGCACTC TGAAACCACC 
TACTCGGTCA CCGTGGCAGC ATACACCACC AAAGGAGACG GAGCGCGCAG CAAAGCCAAA 
GTCATCACCA CGACGGGTGC AGTGCCAGGA AAGCCCACCA TGATGATCAG CACCACTATG 
GGCAACACAG CGCTGATTCA GTGGCAGCCG CCTAAGGAAA TGGTTGGGGA GCTGATGGGC 
TACCAGCTGC AGTACAAGCG CACTGACGAG GAAAACTACA CCTCCCGCGA GCTGAGAAAG 
ATCGATGACC ATTACACCGT CACCGGGCTG CACAAAGGTG CCACCTATGT CTTCCGCCTC 
AGTGCCCGCA ACCGCGCGGG CATCGGCGAA GCATACGTGA AAGAGATCAG CACCCCTGAA 
GACGTGCCGT CGGGCTTCCC GTTCAACCTG CAGGTCACGG GACTGACTCA GTCCAGCACC 
GAGCTGACCT GGGACCCTCC GCTGGTGGCC GAAAGGAACG GCAAGATCGT GCACTATGTG 
GTCGTTTACA GGGACATCAA CAGTCAGCAG AACAGCACCA ACCGGACGAG CGAAACGCAC 
ATGACCATCC AGGGCCTTAA TCCCGACACC ACCTACGATA TCCGGGTGCA GGCGTTCACC 
AGTAAGGGCG GAGGACCCCT CAGCCCTAGC ATCCAGAGCA GGACCATGTC GAACGATTTT 
CCCACCCTCA ACTTTGGTGT CAAAGCCGTC ATGAAAACAT CTGTCCTCCT CACCTGGGAT 
TTGCCACCGA ACTACAAATC CCAAGTGCCT TTCAAGATCC TGTACAACCA GCAAAGCGTG 
GAAGTGCAGG GATCCCTGAA GAGGAGGCTG ATCACGCAGC TGCAGCCAGA CACGGACTAC 
TCTTTTGTGC TGACAAGCAG GGGCAACATT GCTGGTGGTC TACAGCAGCA GGTGTCCATC 
CGCACCGCTC CCGACCTGAT CAACGCCAAG CCCAGCACAC ATCAGCCTCA GGGTAGCAAG 
ATGACAATCA ACCTGCCCAA GGTCCAGACC ACCACGCGTG TCAGGTGGTA CTACATCGTG 
GTGGTGCCGG TGTCCCAGTC AACGCAGGGG TGGAAGAATC CAGACGAGAT GGACCTGGAC 
GAGCTGTTCA AGTCAAGTGA AGGCCCCGTC CTGAGGCGCA GACGTCACCT GGATTCCTCC 
AAACCTTATA TCGCTGCCAA GTTGGCCTCG TTGCCGACCA CCTTCACCCT GGGTGATGAG 
AAGAGATACA ACGGCTTCTA CAACAAGCCC CTGAGCAGTC CGCAGGAGTA TCTCTGCTTT 
GTCCTGGCAG TACTCCGATC CGAAGGGGCA GACAGCACTA CTAATTATAT GACATTTTCA 
GCCAGTCCCT ACTCAGACCC GGTCCACGTG CGAAACAGCA TGGCTCAGGG GATGGAGCAG 
CCTGAAATGT TGTGGGTTAT GGGCCCTGTT TTGGCCGTGG TCCTGATTAT CATCATCGTC 
ATCGCCATCC TGCTCTTCAA GAAGAAGCGA GCGTCGCCCC TACCCAAAGA TGAGCACTCG 
GGCGGGGTGA AGGACTCCCT GCTGGCCAAC TCCTCCGACC CTGTGGAGAT GAGAAGACTT 
AACTACCAGA CCCCAGGAAT GAGGGAGCAC CCGCCCATCC CTGTCGTCGA CCTGGCTGAC 
CACATAGAGC GTCTGAAGGC CAACGACGGC CTACGCTTTT CCCAGGAGTA CGAGTCGATT 
GATCCAGGGC AGCAGTTTAC CTGGGAGAAC TCCAACATGG AAGTCAACAA GCCAAAGAAT 
CGCTATGCAA ACGTCATCGC CTATGACCAC TCCAGAGTGG TTCTCACCTC TGTTGACGCC 
GTTCCAGGCA GTGACTACAT CAACGCCAAC TACATCGACG GCTACAGGAA GCAGAACGCC 
TACATCGCCA CGCAGGGGCC ACTTCCAGAG ACACTGAGCG ACTTCTGGAG GATGGTGTGG 
GAGCAAAGAT CCAACACCAT CGTCATGATG ACCAGACTGG AGGAGAAGTC GCGGGTGAAG 
TGTGACCAGT ACTGGCCGTC GCGAGGAACG GAGACCTATG GGATGATCCA GGTCACCATG 
CTGGACACCG TGGAGTTGGC CACTTACAGC GTCCGTACCT TTGCCCTCTA TAAGAATGGC 
TCCAGTGAGA AGAGAGAGGT GAGACAGTTC CAGTTCATGG CCTGGCCAGA CCACGGGGTG 
CCAGAGTACC CCACCCCAAT CCTGGCCTTC CTACGGCGGG TAAAGGCCTG CAATCCTCCA 
GACGCAGGGC CTATGGTGGT CCACTGCAGC GCGGGTGTGG GCAGGACCGG ATGCTTCATC 
GTGATAGATG CCATGCTGGA GCGCATGAAG CACGAGAAGT CCGTAGACAT CTACGGCCAC 
GTGACGTGCA TGCGCGCCCA GAGGAACTAC ATGGTCCAGA CCGAGGACCA GTACATCTTC 
ATTCACGAAG CCCTGCTGGA GGCCGCCACG TGCGGCAACA CCGAGGTGCC GGCGCGCAAC 
CTGTACGCCC ACATCCAGAA ACTCACCCAG CCCCCGCCCG GCGAGACGGT TACTGCCATG 
GAGCTGGAAT TCAAGAGACT GGCCAACTCC AAAGCCCACA CATCCCGCTT CATCAGCGCC 
AACTTGCCCT GCAACAAGTT CAAGAACCGC CTGGTCAACA TCATGCCTTT CGAGTCCACC 
CGCGTCTGCC TGCAGCCCAT CCGTGGCGTC GAGGGCTCCG ACTACATCAA TGCCAGCTGC 
ATCGACGGCT ACAGACAGCA GAAGGCCTAC CTGGCCACGC AGGGCCCACT GGCAGAGACC 
ACCGAGGACT TCTGGAGGAT GCTGTGGGAG CACAACTCCA CCATCGTCGT CATGCTCACC 
AAGCTACGAG AGATGGGCCG GGAGAAGTGC CATCAGTACT GGCCAGCAGA GCGTTCAGCC 
AGGTACCAGT ACTTTGTGGT GGATCCAATG GCTGAGTACA ACATGCCCCA GTACATCCTC 
AGAGAGTTCA AAGTCACAGA TGCCAGGGAC GGCCAGTCCA GAACAATCCG CCAATTCCAG 
TTCACTGACT GGCCCGAGCA AGGAGTGCCA AAAACCGGAG AGGGCTTCAT TGATTTTATC 
GGACAAGTGC ACAAAACCAA GGAACAGTTC GGACAGGACG GACCAATCAC TGTACACTGC 
AGTGCTGGGG TGGGCAGGAC CGGAGTCTTC ATCACCCTCA GCATCGTGCT GGAGAGGATG 
AGGTATGAAG GAGTTGTCGA CCTCTTCCAG ACAGTGAAGA CACTTCGCAC CCAGCGGCCT 
GCCATGGTGC AGACCGAGGA CCAGTACCAG TTGTGCTACA GGGCAGCATT GGAGTACCTG 
GGGAGCTTTG ACCACTATGC AACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019119102.1
CDS	485..6211
Translation	MVPNIHLAVPFLPRGALLFLILLSCVVFSSLADSMPSFVKSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVEKTRTATMLCAASGNPDPEISWFKDMLPVDISSSNGRIKQLRSGGTPIRGALQIENSEESDQGKYECVAMNSAGTRYSAPANLYVRVRRVPPRFSIPPTNQEVMPGGSVNLTCVAVGSPMPYVKWTMGLVELTKEEEMPMGRNVLEVNNIRESANYTCVAISSLGMIEATAQVTVKALPKPPASLIVTETTATSVTLTWDSGNPEPVSYYMIQYRAKTSDNGFQEVEGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSDPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRVYYSSDLTAPLSAWQKHNTDDSSLTTISGLTPDITYSLRVLGFTSVGDGPPSDILQVKTQQGVPAQPSSFEAEAELDSRIMLTWLWPVQDPITKYELQYWEAGSDNKLHVSFNPAGSYAVDGLKPDTLYRFSLAARSEMGLGVYTQPIEARTAQSTPSAPPQEVHLVSLSSTSLKVSWVAPPAASRHGAIVRYTVSYQAMAGEDTERHEVTGIGADATSFVLEGLEKWTEYQVWVRAHTDVGPGPESAPVRMRTKEDVPGAPPRKLEVEAINSTAIRVTWKPPLQGKQHGQIRGYQVIFSRLENGEPRGQPNIMDVALPEAQWNIEESTEHEAIIAGLHSETTYSVTVAAYTTKGDGARSKAKVITTTGAVPGKPTMMISTTMGNTALIQWQPPKEMVGELMGYQLQYKRTDEENYTSRELRKIDDHYTVTGLHKGATYVFRLSARNRAGIGEAYVKEISTPEDVPSGFPFNLQVTGLTQSSTELTWDPPLVAERNGKIVHYVVVYRDINSQQNSTNRTSETHMTIQGLNPDTTYDIRVQAFTSKGGGPLSPSIQSRTMSNDFPTLNFGVKAVMKTSVLLTWDLPPNYKSQVPFKILYNQQSVEVQGSLKRRLITQLQPDTDYSFVLTSRGNIAGGLQQQVSIRTAPDLINAKPSTHQPQGSKMTINLPKVQTTTRVRWYYIVVVPVSQSTQGWKNPDEMDLDELFKSSEGPVLRRRRHLDSSKPYIAAKLASLPTTFTLGDEKRYNGFYNKPLSSPQEYLCFVLAVLRSEGADSTTNYMTFSASPYSDPVHVRNSMAQGMEQPEMLWVMGPVLAVVLIIIIVIAILLFKKKRASPLPKDEHSGGVKDSLLANSSDPVEMRRLNYQTPGMREHPPIPVVDLADHIERLKANDGLRFSQEYESIDPGQQFTWENSNMEVNKPKNRYANVIAYDHSRVVLTSVDAVPGSDYINANYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRSNTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVTMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRAQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPPPGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYLATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_019263557.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019263557.2

ORF Insert Sequence:

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTGGACATC 
AGCAGCAGCA ACGGGCGCAT TAAACAGCTT CGTTCAGGTG GTACACCAAT TAGAGGAGCT 
CTGCAAATTG AGAACAGCGA GGAGTCCGAC CAAGGGAAAT ACGAGTGTGT TGCCATGAAC 
AGCGCTGGGA CCCGTTACTC CGCTCCTGCC AATCTCTACG TACGAGTGCG AAGAGTCCCA 
CCCAGATTCT CCATTCCGCC CACCAACCAA GAGGTGATGC CAGGCGGCAG CGTCAACCTG 
ACATGCGTGG CAGTGGGCTC GCCGATGCCC TACGTCAAGT GGACGATGGG CCTGGTGGAG 
CTGACCAAGG AGGAGGAGAT GCCGATGGGA CGCAACGTGC TGGAGGTCAA CAACATCCGC 
GAGTCGGCGA ACTACACCTG CGTGGCCATC TCCTCTCTGG GCATGATAGA AGCCACCGCT 
CAGGTCACCG TCAAAGCTTT GCCAAAGCCT CCCGCCTCCC TCATTGTGAC TGAGACCACA 
GCCACCAGTG TGACTCTCAC CTGGGACTCT GGAAACCCAG AACCTGTCTC CTACTACATG 
ATCCAATACA GAGCCAAGAC CTCGGACAAC GGCTTCCAGG AAGTGGAAGG TGTGGCAACT 
ACCCGTTACA GCATCGGTGG ACTTAGCCCC TACTCTGAGT ATGAGTTTCG TGTCATGGCT 
GTCAACAACA TCGGCCGGGG GCCACCGAGC GACCCCGTGG AGACACGCAC TGGTGAACAG 
GCCCCCTCCT CGCCACCTCT GCACGTCCAA GCGCGCATGC TGAGTGCCAG CACAATGCTG 
GTCCAGTGGG AACCGCCCGA GGAACCCAAC GGGCAAATCA GGGGCTACCG GGTCTACTAC 
AGCTCGGACT TGACGGCTCC GCTCAGCGCC TGGCAGAAGC ACAACACAGA CGACAGCAGC 
CTGACGACCA TCTCGGGCCT GACTCCTGAT ATCACCTACA GCCTCAGGGT GCTGGGCTTC 
ACCTCTGTAG GTGACGGGCC ACCTTCTGAC ATCCTGCAAG TCAAAACCCA ACAGGGAGTC 
CCTGCCCAGC CGTCCAGCTT TGAGGCCGAA GCTGAGCTGG ACAGTCGCAT CATGCTAACA 
TGGCTGTGGC CCGTCCAGGA CCCCATCACT AAGTATGAGC TGCAGTACTG GGAGGCCGGC 
TCAGACAACA AGTTACATGT GAGCTTCAAC CCAGCGGGCT CCTACGCTGT GGACGGTCTA 
AAGCCTGACA CCTTGTACCG TTTCTCTCTG GCAGCCCGTT CTGAGATGGG CTTAGGTGTC 
TACACCCAAC CCATCGAGGC TCGCACCGCC CAGTCCACCC CATCAGCTCC CCCCCAGGAG 
GTACACCTGG TCAGCCTGAG CTCCACCAGC CTCAAGGTGA GTTGGGTGGC ACCGCCCGCC 
GCTAGCCGCC ACGGCGCCAT TGTGCGCTAC ACAGTCAGCT ACCAGGCCAT GGCTGGAGAA 
GACACGGAGC GGCACGAGGT GACGGGCATC GGCGCAGACG CCACTAGCTT TGTGCTGGAG 
GGCCTGGAGA AGTGGACTGA GTATCAGGTG TGGGTGAGGG CACACACTGA CGTGGGCCCC 
GGCCCAGAGA GTGCCCCAGT GAGGATGAGA ACCAAAGAGG ATGTGCCGGG AGCACCCCCC 
AGGAAGCTGG AGGTTGAGGC CATAAACTCC ACAGCAATCC GGGTCACCTG GAAGCCGCCC 
CTGCAGGGGA AGCAGCACGG CCAAATCCGG GGCTATCAAG TCATCTTCTC TCGGCTTGAA 
AACGGCGAAC CACGAGGCCA GCCTAACATC ATGGACGTTG CTTTGCCAGA GGCACAGTGG 
AATATTGAGG AGTCCACCGA GCATGAGGCC ATTATTGCCG GACTGCACTC TGAAACCACC 
TACTCGGTCA CCGTGGCAGC ATACACCACC AAAGGAGACG GAGCGCGCAG CAAAGCCAAA 
GTCATCACCA CGACGGGTGC AGTGCCAGGA AAGCCCACCA TGATGATCAG CACCACTATG 
GGCAACACAG CGCTGATTCA GTGGCAGCCG CCTAAGGAAA TGGTTGGGGA GCTGATGGGC 
TACCAGCTGC AGTACAAGCG CACTGACGAG GAAAACTACA CCTCCCGCGA GCTGAGAAAG 
ATCGATGACC ATTACACCGT CACCGGGCTG CACAAAGGTG CCACCTATGT CTTCCGCCTC 
AGTGCCCGCA ACCGCGCGGG CATCGGCGAA GCATACGTGA AAGAGATCAG CACCCCTGAA 
GACGTGCCGT CGGGCTTCCC GTTCAACCTG CAGGTCACGG GACTGACTCA GTCCAGCACC 
GAGCTGACCT GGGACCCTCC GCTGGTGGCC GAAAGGAACG GCAAGATCGT GCACTATGTG 
GTCGTTTACA GGGACATCAA CAGTCAGCAG AACAGCACCA ACCGGACGAG CGAAACGCAC 
ATGACCATCC AGGGCCTTAA TCCCGACACC ACCTACGATA TCCGGGTGCA GGCGTTCACC 
AGTAAGGGCG GAGGACCCCT CAGCCCTAGC ATCCAGAGCA GGACCATGTC GAACGATTTT 
CCCACCCTCA ACTTTGGTGT CAAAGCCGTC ATGAAAACAT CTGTCCTCCT CACCTGGGAT 
TTGCCACCGA ACTACAAATC CCAAGTGCCT TTCAAGATCC TGTACAACCA GCAAAGCGTG 
GAAGTGCAGG GATCCCTGAA GAGGAGGCTG ATCACGCAGC TGCAGCCAGA CACGGACTAC 
TCTTTTGTGC TGACAAGCAG GGGCAACATT GCTGGTGGTC TACAGCAGCA GGTGTCCATC 
CGCACCGCTC CCGACCTGAT CAACGCCAAG CCCAGCACAC ATCAGCCTCA GGGTAGCAAG 
ATGACAATCA ACCTGCCCAA GGTCCAGACC ACCACGCGTG TCAGGTGGTA CTACATCGTG 
GTGGTGCCGG TGTCCCAGTC AACGCAGGGG TGGAAGAATC CAGACGAGAT GGACCTGGAC 
GAGCTGTTCA AGTCAAGTGA AGGCCCCGTC CTGAGGCGCA GACGTCACCT GGATTCCTCC 
AAACCTTATA TCGCTGCCAA GTTGGCCTCG TTGCCGACCA CCTTCACCCT GGGTGATGAG 
AAGAGATACA ACGGCTTCTA CAACAAGCCC CTGAGCAGTC CGCAGGAGTA TCTCTGCTTT 
GTCCTGGCAG TACTCCGATC CGAAGGGGCA GACAGCACTA CTAATTATAT GACATTTTCA 
GCCAGTCCCT ACTCAGACCC GGTCCACGTG CGAAACAGCA TGGCTCAGGG GATGGAGCAG 
CCTGAAATGT TGTGGGTTAT GGGCCCTGTT TTGGCCGTGG TCCTGATTAT CATCATCGTC 
ATCGCCATCC TGCTCTTCAA GAAGAAGCGA GCGTCGCCCC TACCCAAAGA TGAGCACTCG 
GGCGGGGTGA AGGACTCCCT GCTGGCCAAC TCCTCCGACC CTGTGGAGAT GAGAAGACTT 
AACTACCAGA CCCCAGGAAT GAGGGAGCAC CCGCCCATCC CTGTCGTCGA CCTGGCTGAC 
CACATAGAGC GTCTGAAGGC CAACGACGGC CTACGCTTTT CCCAGGAGTA CGAGTCGATT 
GATCCAGGGC AGCAGTTTAC CTGGGAGAAC TCCAACATGG AAGTCAACAA GCCAAAGAAT 
CGCTATGCAA ACGTCATCGC CTATGACCAC TCCAGAGTGG TTCTCACCTC TGTTGACGCC 
GTTCCAGGCA GTGACTACAT CAACGCCAAC TACATCGACG GCTACAGGAA GCAGAACGCC 
TACATCGCCA CGCAGGGGCC ACTTCCAGAG ACACTGAGCG ACTTCTGGAG GATGGTGTGG 
GAGCAAAGAT CCAACACCAT CGTCATGATG ACCAGACTGG AGGAGAAGTC GCGGGTGAAG 
TGTGACCAGT ACTGGCCGTC GCGAGGAACG GAGACCTATG GGATGATCCA GGTCACCATG 
CTGGACACCG TGGAGTTGGC CACTTACAGC GTCCGTACCT TTGCCCTCTA TAAGAATGGC 
TCCAGTGAGA AGAGAGAGGT GAGACAGTTC CAGTTCATGG CCTGGCCAGA CCACGGGGTG 
CCAGAGTACC CCACCCCAAT CCTGGCCTTC CTACGGCGGG TAAAGGCCTG CAATCCTCCA 
GACGCAGGGC CTATGGTGGT CCACTGCAGC GCGGGTGTGG GCAGGACCGG ATGCTTCATC 
GTGATAGATG CCATGCTGGA GCGCATGAAG CACGAGAAGT CCGTAGACAT CTACGGCCAC 
GTGACGTGCA TGCGCGCCCA GAGGAACTAC ATGGTCCAGA CCGAGGACCA GTACATCTTC 
ATTCACGAAG CCCTGCTGGA GGCCGCCACG TGCGGCAACA CCGAGGTGCC GGCGCGCAAC 
CTGTACGCCC ACATCCAGAA ACTCACCCAG CCCCCGCCCG GCGAGACGGT TACTGCCATG 
GAGCTGGAAT TCAAGAGACT GGCCAACTCC AAAGCCCACA CATCCCGCTT CATCAGCGCC 
AACTTGCCCT GCAACAAGTT CAAGAACCGC CTGGTCAACA TCATGCCTTT CGAGTCCACC 
CGCGTCTGCC TGCAGCCCAT CCGTGGCGTC GAGGGCTCCG ACTACATCAA TGCCAGCTGC 
ATCGACGGCT ACAGACAGCA GAAGGCCTAC CTGGCCACGC AGGGCCCACT GGCAGAGACC 
ACCGAGGACT TCTGGAGGAT GCTGTGGGAG CACAACTCCA CCATCGTCGT CATGCTCACC 
AAGCTACGAG AGATGGGCCG GGAGAAGTGC CATCAGTACT GGCCAGCAGA GCGTTCAGCC 
AGGTACCAGT ACTTTGTGGT GGATCCAATG GCTGAGTACA ACATGCCCCA GTACATCCTC 
AGAGAGTTCA AAGTCACAGA TGCCAGGGAC GGCCAGTCCA GAACAATCCG CCAATTCCAG 
TTCACTGACT GGCCCGAGCA AGGAGTGCCA AAAACCGGAG AGGGCTTCAT TGATTTTATC 
GGACAAGTGC ACAAAACCAA GGAACAGTTC GGACAGGACG GACCAATCAC TGTACACTGC 
AGTGCTGGGG TGGGCAGGAC CGGAGTCTTC ATCACCCTCA GCATCGTGCT GGAGAGGATG 
AGGTATGAAG GAGTTGTCGA CCTCTTCCAG ACAGTGAAGA CACTTCGCAC CCAGCGGCCT 
GCCATGGTGC AGACCGAGGA CCAGTACCAG TTGTGCTACA GGGCAGCATT GGAGTACCTG 
GGGAGCTTTG ACCACTATGC AACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104931439 ( XM_019263564.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019119109.1 Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X9, mRNA.

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CloneID	OLa211353
Clone ID Related Accession (Same CDS sequence)	XM_019263564.2
Accession Version	XM_019263564.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	receptor-type tyrosine-protein phosphatase F isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040012.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_019263564.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTGGACATC 
AGCAGCAGCA ACGGGCGCAT TAAACAGCTT CGTTCAGGTG GTACACCAAT TAGAGGAGCT 
CTGCAAATTG AGAACAGCGA GGAGTCCGAC CAAGGGAAAT ACGAGTGTGT TGCCATGAAC 
AGCGCTGGGA CCCGTTACTC CGCTCCTGCC AATCTCTACG TACGAGTGCG AAGAGTCCCA 
CCCAGATTCT CCATTCCGCC CACCAACCAA GAGGTGATGC CAGGCGGCAG CGTCAACCTG 
ACATGCGTGG CAGTGGGCTC GCCGATGCCC TACGTCAAGT GGACGATGGG CCTGGTGGAG 
CTGACCAAGG AGGAGGAGAT GCCGATGGGA CGCAACGTGC TGGAGGTCAA CAACATCCGC 
GAGTCGGCGA ACTACACCTG CGTGGCCATC TCCTCTCTGG GCATGATAGA AGCCACCGCT 
CAGGTCACCG TCAAAGCTTT GCCAAAGCCT CCCGCCTCCC TCATTGTGAC TGAGACCACA 
GCCACCAGTG TGACTCTCAC CTGGGACTCT GGAAACCCAG AACCTGTCTC CTACTACATG 
ATCCAATACA GAGCCAAGAC CTCGGACAAC GGCTTCCAGG AAGTGGAAGG TGTGGCAACT 
ACCCGTTACA GCATCGGTGG ACTTAGCCCC TACTCTGAGT ATGAGTTTCG TGTCATGGCT 
GTCAACAACA TCGGCCGGGG GCCACCGAGC GACCCCGTGG AGACACGCAC TGGTGAACAG 
GCCCCCTCCT CGCCACCTCT GCACGTCCAA GCGCGCATGC TGAGTGCCAG CACAATGCTG 
GTCCAGTGGG AACCGCCCGA GGAACCCAAC GGGCAAATCA GGGGCTACCG GGTCTACTAC 
AGCTCGGACT TGACGGCTCC GCTCAGCGCC TGGCAGAAGC ACAACACAGA CGACAGCAGC 
CTGACGACCA TCTCGGGCCT GACTCCTGAT ATCACCTACA GCCTCAGGGT GCTGGGCTTC 
ACCTCTGTAG GTGACGGGCC ACCTTCTGAC ATCCTGCAAG TCAAAACCCA ACAGGGAGTC 
CCTGCCCAGC CGTCCAGCTT TGAGGCCGAA GCTGAGCTGG ACAGTCGCAT CATGCTAACA 
TGGCTGTGGC CCGTCCAGGA CCCCATCACT AAGTATGAGC TGCAGTACTG GGAGGCCGGC 
TCAGACAACA AGTTACATGT GAGCTTCAAC CCAGCGGGCT CCTACGCTGT GGACGGTCTA 
AAGCCTGACA CCTTGTACCG TTTCTCTCTG GCAGCCCGTT CTGAGATGGG CTTAGGTGTC 
TACACCCAAC CCATCGAGGC TCGCACCGCC CAGTCCATGC CGGGAGCACC CCCCAGGAAG 
CTGGAGGTTG AGGCCATAAA CTCCACAGCA ATCCGGGTCA CCTGGAAGCC GCCCCTGCAG 
GGGAAGCAGC ACGGCCAAAT CCGGGGCTAT CAAGTCATCT TCTCTCGGCT TGAAAACGGC 
GAACCACGAG GCCAGCCTAA CATCATGGAC GTTGCTTTGC CAGAGGCACA GTGGAATATT 
GAGGAGTCCA CCGAGCATGA GGCCATTATT GCCGGACTGC ACTCTGAAAC CACCTACTCG 
GTCACCGTGG CAGCATACAC CACCAAAGGA GACGGAGCGC GCAGCAAAGC CAAAGTCATC 
ACCACGACGG GTGCAGTGCC AGGAAAGCCC ACCATGATGA TCAGCACCAC TATGGGCAAC 
ACAGCGCTGA TTCAGTGGCA GCCGCCTAAG GAAATGGTTG GGGAGCTGAT GGGCTACCAG 
CTGCAGTACA AGCGCACTGA CGAGGAAAAC TACACCTCCC GCGAGCTGAG AAAGATCGAT 
GACCATTACA CCGTCACCGG GCTGCACAAA GGTGCCACCT ATGTCTTCCG CCTCAGTGCC 
CGCAACCGCG CGGGCATCGG CGAAGCATAC GTGAAAGAGA TCAGCACCCC TGAAGACGTG 
CCGTCGGGCT TCCCGTTCAA CCTGCAGGTC ACGGGACTGA CTCAGTCCAG CACCGAGCTG 
ACCTGGGACC CTCCGCTGGT GGCCGAAAGG AACGGCAAGA TCGTGCACTA TGTGGTCGTT 
TACAGGGACA TCAACAGTCA GCAGAACAGC ACCAACCGGA CGAGCGAAAC GCACATGACC 
ATCCAGGGCC TTAATCCCGA CACCACCTAC GATATCCGGG TGCAGGCGTT CACCAGTAAG 
GGCGGAGGAC CCCTCAGCCC TAGCATCCAG AGCAGGACCA TGTCGAACGA TTTTCCCACC 
CTCAACTTTG GTGTCAAAGC CGTCATGAAA ACATCTGTCC TCCTCACCTG GGATTTGCCA 
CCGAACTACA AATCCCAAGT GCCTTTCAAG ATCCTGTACA ACCAGCAAAG CGTGGAAGTG 
CAGGGATCCC TGAAGAGGAG GCTGATCACG CAGCTGCAGC CAGACACGGA CTACTCTTTT 
GTGCTGACAA GCAGGGGCAA CATTGCTGGT GGTCTACAGC AGCAGGTGTC CATCCGCACC 
GCTCCCGACC TGATCAACGC CAAGCCCAGC ACACATCAGC CTCAGGGTAG CAAGATGACA 
ATCAACCTGC CCAAGGTCCA GACCACCACG CGTGTCAGGT GGTACTACAT CGTGGTGGTG 
CCGGTGTCCC AGTCAACGCA GGGGTGGAAG AATCCAGACG AGATGGACCT GGACGAGCTG 
TTCAAGTCAA GTGAAGGCCC CGTCCTGAGG CGCAGACGTC ACCTGGATTC CTCCAAACCT 
TATATCGCTG CCAAGTTGGC CTCGTTGCCG ACCACCTTCA CCCTGGGTGA TGAGAAGAGA 
TACAACGGCT TCTACAACAA GCCCCTGAGC AGTCCGCAGG AGTATCTCTG CTTTGTCCTG 
GCAGTACTCC GATCCGAAGG GGCAGACAGC ACTACTAATT ATATGACATT TTCAGCCAGT 
CCCTACTCAG ACCCGGTCCA CGTGCGAAAC AGCATGGCTC AGGGGATGGA GCAGCCTGAA 
ATGTTGTGGG TTATGGGCCC TGTTTTGGCC GTGGTCCTGA TTATCATCAT CGTCATCGCC 
ATCCTGCTCT TCAAGAACAA ACAGGAAAGG AAGCGAGCGT CGCCCCTACC CAAAGATGAG 
CACTCGGGCG GGGTGAAGGA CTCCCTGCTG GCCAACTCCT CCGACCCTGT GGAGATGAGA 
AGACTTAACT ACCAGACCCC AGGTCCCAGC TCTCATCGCT GCCCCAACAC TCCAAGAATG 
AGGGAGCACC CGCCCATCCC TGTCGTCGAC CTGGCTGACC ACATAGAGCG TCTGAAGGCC 
AACGACGGCC TACGCTTTTC CCAGGAGTAC GAGTCGATTG ATCCAGGGCA GCAGTTTACC 
TGGGAGAACT CCAACATGGA AGTCAACAAG CCAAAGAATC GCTATGCAAA CGTCATCGCC 
TATGACCACT CCAGAGTGGT TCTCACCTCT GTTGACGCCG TTCCAGGCAG TGACTACATC 
AACGCCAACT ACATCGACGG CTACAGGAAG CAGAACGCCT ACATCGCCAC GCAGGGGCCA 
CTTCCAGAGA CACTGAGCGA CTTCTGGAGG ATGGTGTGGG AGCAAAGATC CAACACCATC 
GTCATGATGA CCAGACTGGA GGAGAAGTCG CGGGTGAAGT GTGACCAGTA CTGGCCGTCG 
CGAGGAACGG AGACCTATGG GATGATCCAG GTCACCATGC TGGACACCGT GGAGTTGGCC 
ACTTACAGCG TCCGTACCTT TGCCCTCTAT AAGAATGGCT CCAGTGAGAA GAGAGAGGTG 
AGACAGTTCC AGTTCATGGC CTGGCCAGAC CACGGGGTGC CAGAGTACCC CACCCCAATC 
CTGGCCTTCC TACGGCGGGT AAAGGCCTGC AATCCTCCAG ACGCAGGGCC TATGGTGGTC 
CACTGCAGCG CGGGTGTGGG CAGGACCGGA TGCTTCATCG TGATAGATGC CATGCTGGAG 
CGCATGAAGC ACGAGAAGTC CGTAGACATC TACGGCCACG TGACGTGCAT GCGCGCCCAG 
AGGAACTACA TGGTCCAGAC CGAGGACCAG TACATCTTCA TTCACGAAGC CCTGCTGGAG 
GCCGCCACGT GCGGCAACAC CGAGGTGCCG GCGCGCAACC TGTACGCCCA CATCCAGAAA 
CTCACCCAGC CCCCGCCCGG CGAGACGGTT ACTGCCATGG AGCTGGAATT CAAGAGACTG 
GCCAACTCCA AAGCCCACAC ATCCCGCTTC ATCAGCGCCA ACTTGCCCTG CAACAAGTTC 
AAGAACCGCC TGGTCAACAT CATGCCTTTC GAGTCCACCC GCGTCTGCCT GCAGCCCATC 
CGTGGCGTCG AGGGCTCCGA CTACATCAAT GCCAGCTGCA TCGACGGCTA CAGACAGCAG 
AAGGCCTACC TGGCCACGCA GGGCCCACTG GCAGAGACCA CCGAGGACTT CTGGAGGATG 
CTGTGGGAGC ACAACTCCAC CATCGTCGTC ATGCTCACCA AGCTACGAGA GATGGGCCGG 
GAGAAGTGCC ATCAGTACTG GCCAGCAGAG CGTTCAGCCA GGTACCAGTA CTTTGTGGTG 
GATCCAATGG CTGAGTACAA CATGCCCCAG TACATCCTCA GAGAGTTCAA AGTCACAGAT 
GCCAGGGACG GCCAGTCCAG AACAATCCGC CAATTCCAGT TCACTGACTG GCCCGAGCAA 
GGAGTGCCAA AAACCGGAGA GGGCTTCATT GATTTTATCG GACAAGTGCA CAAAACCAAG 
GAACAGTTCG GACAGGACGG ACCAATCACT GTACACTGCA GTGCTGGGGT GGGCAGGACC 
GGAGTCTTCA TCACCCTCAG CATCGTGCTG GAGAGGATGA GGTATGAAGG AGTTGTCGAC 
CTCTTCCAGA CAGTGAAGAC ACTTCGCACC CAGCGGCCTG CCATGGTGCA GACCGAGGAC 
CAGTACCAGT TGTGCTACAG GGCAGCATTG GAGTACCTGG GGAGCTTTGA CCACTATGCA 
ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019119109.1
CDS	483..5948
Translation	MVPNIHLAVPFLPRGALLFLILLSCVVFSSLADSMPSFVKSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVEKTRTATMLCAASGNPDPEISWFKDMLPVDISSSNGRIKQLRSGGTPIRGALQIENSEESDQGKYECVAMNSAGTRYSAPANLYVRVRRVPPRFSIPPTNQEVMPGGSVNLTCVAVGSPMPYVKWTMGLVELTKEEEMPMGRNVLEVNNIRESANYTCVAISSLGMIEATAQVTVKALPKPPASLIVTETTATSVTLTWDSGNPEPVSYYMIQYRAKTSDNGFQEVEGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSDPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRVYYSSDLTAPLSAWQKHNTDDSSLTTISGLTPDITYSLRVLGFTSVGDGPPSDILQVKTQQGVPAQPSSFEAEAELDSRIMLTWLWPVQDPITKYELQYWEAGSDNKLHVSFNPAGSYAVDGLKPDTLYRFSLAARSEMGLGVYTQPIEARTAQSMPGAPPRKLEVEAINSTAIRVTWKPPLQGKQHGQIRGYQVIFSRLENGEPRGQPNIMDVALPEAQWNIEESTEHEAIIAGLHSETTYSVTVAAYTTKGDGARSKAKVITTTGAVPGKPTMMISTTMGNTALIQWQPPKEMVGELMGYQLQYKRTDEENYTSRELRKIDDHYTVTGLHKGATYVFRLSARNRAGIGEAYVKEISTPEDVPSGFPFNLQVTGLTQSSTELTWDPPLVAERNGKIVHYVVVYRDINSQQNSTNRTSETHMTIQGLNPDTTYDIRVQAFTSKGGGPLSPSIQSRTMSNDFPTLNFGVKAVMKTSVLLTWDLPPNYKSQVPFKILYNQQSVEVQGSLKRRLITQLQPDTDYSFVLTSRGNIAGGLQQQVSIRTAPDLINAKPSTHQPQGSKMTINLPKVQTTTRVRWYYIVVVPVSQSTQGWKNPDEMDLDELFKSSEGPVLRRRRHLDSSKPYIAAKLASLPTTFTLGDEKRYNGFYNKPLSSPQEYLCFVLAVLRSEGADSTTNYMTFSASPYSDPVHVRNSMAQGMEQPEMLWVMGPVLAVVLIIIIVIAILLFKNKQERKRASPLPKDEHSGGVKDSLLANSSDPVEMRRLNYQTPGPSSHRCPNTPRMREHPPIPVVDLADHIERLKANDGLRFSQEYESIDPGQQFTWENSNMEVNKPKNRYANVIAYDHSRVVLTSVDAVPGSDYINANYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRSNTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVTMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRAQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPPPGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYLATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_019263564.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019263564.2

ORF Insert Sequence:

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTGGACATC 
AGCAGCAGCA ACGGGCGCAT TAAACAGCTT CGTTCAGGTG GTACACCAAT TAGAGGAGCT 
CTGCAAATTG AGAACAGCGA GGAGTCCGAC CAAGGGAAAT ACGAGTGTGT TGCCATGAAC 
AGCGCTGGGA CCCGTTACTC CGCTCCTGCC AATCTCTACG TACGAGTGCG AAGAGTCCCA 
CCCAGATTCT CCATTCCGCC CACCAACCAA GAGGTGATGC CAGGCGGCAG CGTCAACCTG 
ACATGCGTGG CAGTGGGCTC GCCGATGCCC TACGTCAAGT GGACGATGGG CCTGGTGGAG 
CTGACCAAGG AGGAGGAGAT GCCGATGGGA CGCAACGTGC TGGAGGTCAA CAACATCCGC 
GAGTCGGCGA ACTACACCTG CGTGGCCATC TCCTCTCTGG GCATGATAGA AGCCACCGCT 
CAGGTCACCG TCAAAGCTTT GCCAAAGCCT CCCGCCTCCC TCATTGTGAC TGAGACCACA 
GCCACCAGTG TGACTCTCAC CTGGGACTCT GGAAACCCAG AACCTGTCTC CTACTACATG 
ATCCAATACA GAGCCAAGAC CTCGGACAAC GGCTTCCAGG AAGTGGAAGG TGTGGCAACT 
ACCCGTTACA GCATCGGTGG ACTTAGCCCC TACTCTGAGT ATGAGTTTCG TGTCATGGCT 
GTCAACAACA TCGGCCGGGG GCCACCGAGC GACCCCGTGG AGACACGCAC TGGTGAACAG 
GCCCCCTCCT CGCCACCTCT GCACGTCCAA GCGCGCATGC TGAGTGCCAG CACAATGCTG 
GTCCAGTGGG AACCGCCCGA GGAACCCAAC GGGCAAATCA GGGGCTACCG GGTCTACTAC 
AGCTCGGACT TGACGGCTCC GCTCAGCGCC TGGCAGAAGC ACAACACAGA CGACAGCAGC 
CTGACGACCA TCTCGGGCCT GACTCCTGAT ATCACCTACA GCCTCAGGGT GCTGGGCTTC 
ACCTCTGTAG GTGACGGGCC ACCTTCTGAC ATCCTGCAAG TCAAAACCCA ACAGGGAGTC 
CCTGCCCAGC CGTCCAGCTT TGAGGCCGAA GCTGAGCTGG ACAGTCGCAT CATGCTAACA 
TGGCTGTGGC CCGTCCAGGA CCCCATCACT AAGTATGAGC TGCAGTACTG GGAGGCCGGC 
TCAGACAACA AGTTACATGT GAGCTTCAAC CCAGCGGGCT CCTACGCTGT GGACGGTCTA 
AAGCCTGACA CCTTGTACCG TTTCTCTCTG GCAGCCCGTT CTGAGATGGG CTTAGGTGTC 
TACACCCAAC CCATCGAGGC TCGCACCGCC CAGTCCATGC CGGGAGCACC CCCCAGGAAG 
CTGGAGGTTG AGGCCATAAA CTCCACAGCA ATCCGGGTCA CCTGGAAGCC GCCCCTGCAG 
GGGAAGCAGC ACGGCCAAAT CCGGGGCTAT CAAGTCATCT TCTCTCGGCT TGAAAACGGC 
GAACCACGAG GCCAGCCTAA CATCATGGAC GTTGCTTTGC CAGAGGCACA GTGGAATATT 
GAGGAGTCCA CCGAGCATGA GGCCATTATT GCCGGACTGC ACTCTGAAAC CACCTACTCG 
GTCACCGTGG CAGCATACAC CACCAAAGGA GACGGAGCGC GCAGCAAAGC CAAAGTCATC 
ACCACGACGG GTGCAGTGCC AGGAAAGCCC ACCATGATGA TCAGCACCAC TATGGGCAAC 
ACAGCGCTGA TTCAGTGGCA GCCGCCTAAG GAAATGGTTG GGGAGCTGAT GGGCTACCAG 
CTGCAGTACA AGCGCACTGA CGAGGAAAAC TACACCTCCC GCGAGCTGAG AAAGATCGAT 
GACCATTACA CCGTCACCGG GCTGCACAAA GGTGCCACCT ATGTCTTCCG CCTCAGTGCC 
CGCAACCGCG CGGGCATCGG CGAAGCATAC GTGAAAGAGA TCAGCACCCC TGAAGACGTG 
CCGTCGGGCT TCCCGTTCAA CCTGCAGGTC ACGGGACTGA CTCAGTCCAG CACCGAGCTG 
ACCTGGGACC CTCCGCTGGT GGCCGAAAGG AACGGCAAGA TCGTGCACTA TGTGGTCGTT 
TACAGGGACA TCAACAGTCA GCAGAACAGC ACCAACCGGA CGAGCGAAAC GCACATGACC 
ATCCAGGGCC TTAATCCCGA CACCACCTAC GATATCCGGG TGCAGGCGTT CACCAGTAAG 
GGCGGAGGAC CCCTCAGCCC TAGCATCCAG AGCAGGACCA TGTCGAACGA TTTTCCCACC 
CTCAACTTTG GTGTCAAAGC CGTCATGAAA ACATCTGTCC TCCTCACCTG GGATTTGCCA 
CCGAACTACA AATCCCAAGT GCCTTTCAAG ATCCTGTACA ACCAGCAAAG CGTGGAAGTG 
CAGGGATCCC TGAAGAGGAG GCTGATCACG CAGCTGCAGC CAGACACGGA CTACTCTTTT 
GTGCTGACAA GCAGGGGCAA CATTGCTGGT GGTCTACAGC AGCAGGTGTC CATCCGCACC 
GCTCCCGACC TGATCAACGC CAAGCCCAGC ACACATCAGC CTCAGGGTAG CAAGATGACA 
ATCAACCTGC CCAAGGTCCA GACCACCACG CGTGTCAGGT GGTACTACAT CGTGGTGGTG 
CCGGTGTCCC AGTCAACGCA GGGGTGGAAG AATCCAGACG AGATGGACCT GGACGAGCTG 
TTCAAGTCAA GTGAAGGCCC CGTCCTGAGG CGCAGACGTC ACCTGGATTC CTCCAAACCT 
TATATCGCTG CCAAGTTGGC CTCGTTGCCG ACCACCTTCA CCCTGGGTGA TGAGAAGAGA 
TACAACGGCT TCTACAACAA GCCCCTGAGC AGTCCGCAGG AGTATCTCTG CTTTGTCCTG 
GCAGTACTCC GATCCGAAGG GGCAGACAGC ACTACTAATT ATATGACATT TTCAGCCAGT 
CCCTACTCAG ACCCGGTCCA CGTGCGAAAC AGCATGGCTC AGGGGATGGA GCAGCCTGAA 
ATGTTGTGGG TTATGGGCCC TGTTTTGGCC GTGGTCCTGA TTATCATCAT CGTCATCGCC 
ATCCTGCTCT TCAAGAACAA ACAGGAAAGG AAGCGAGCGT CGCCCCTACC CAAAGATGAG 
CACTCGGGCG GGGTGAAGGA CTCCCTGCTG GCCAACTCCT CCGACCCTGT GGAGATGAGA 
AGACTTAACT ACCAGACCCC AGGTCCCAGC TCTCATCGCT GCCCCAACAC TCCAAGAATG 
AGGGAGCACC CGCCCATCCC TGTCGTCGAC CTGGCTGACC ACATAGAGCG TCTGAAGGCC 
AACGACGGCC TACGCTTTTC CCAGGAGTAC GAGTCGATTG ATCCAGGGCA GCAGTTTACC 
TGGGAGAACT CCAACATGGA AGTCAACAAG CCAAAGAATC GCTATGCAAA CGTCATCGCC 
TATGACCACT CCAGAGTGGT TCTCACCTCT GTTGACGCCG TTCCAGGCAG TGACTACATC 
AACGCCAACT ACATCGACGG CTACAGGAAG CAGAACGCCT ACATCGCCAC GCAGGGGCCA 
CTTCCAGAGA CACTGAGCGA CTTCTGGAGG ATGGTGTGGG AGCAAAGATC CAACACCATC 
GTCATGATGA CCAGACTGGA GGAGAAGTCG CGGGTGAAGT GTGACCAGTA CTGGCCGTCG 
CGAGGAACGG AGACCTATGG GATGATCCAG GTCACCATGC TGGACACCGT GGAGTTGGCC 
ACTTACAGCG TCCGTACCTT TGCCCTCTAT AAGAATGGCT CCAGTGAGAA GAGAGAGGTG 
AGACAGTTCC AGTTCATGGC CTGGCCAGAC CACGGGGTGC CAGAGTACCC CACCCCAATC 
CTGGCCTTCC TACGGCGGGT AAAGGCCTGC AATCCTCCAG ACGCAGGGCC TATGGTGGTC 
CACTGCAGCG CGGGTGTGGG CAGGACCGGA TGCTTCATCG TGATAGATGC CATGCTGGAG 
CGCATGAAGC ACGAGAAGTC CGTAGACATC TACGGCCACG TGACGTGCAT GCGCGCCCAG 
AGGAACTACA TGGTCCAGAC CGAGGACCAG TACATCTTCA TTCACGAAGC CCTGCTGGAG 
GCCGCCACGT GCGGCAACAC CGAGGTGCCG GCGCGCAACC TGTACGCCCA CATCCAGAAA 
CTCACCCAGC CCCCGCCCGG CGAGACGGTT ACTGCCATGG AGCTGGAATT CAAGAGACTG 
GCCAACTCCA AAGCCCACAC ATCCCGCTTC ATCAGCGCCA ACTTGCCCTG CAACAAGTTC 
AAGAACCGCC TGGTCAACAT CATGCCTTTC GAGTCCACCC GCGTCTGCCT GCAGCCCATC 
CGTGGCGTCG AGGGCTCCGA CTACATCAAT GCCAGCTGCA TCGACGGCTA CAGACAGCAG 
AAGGCCTACC TGGCCACGCA GGGCCCACTG GCAGAGACCA CCGAGGACTT CTGGAGGATG 
CTGTGGGAGC ACAACTCCAC CATCGTCGTC ATGCTCACCA AGCTACGAGA GATGGGCCGG 
GAGAAGTGCC ATCAGTACTG GCCAGCAGAG CGTTCAGCCA GGTACCAGTA CTTTGTGGTG 
GATCCAATGG CTGAGTACAA CATGCCCCAG TACATCCTCA GAGAGTTCAA AGTCACAGAT 
GCCAGGGACG GCCAGTCCAG AACAATCCGC CAATTCCAGT TCACTGACTG GCCCGAGCAA 
GGAGTGCCAA AAACCGGAGA GGGCTTCATT GATTTTATCG GACAAGTGCA CAAAACCAAG 
GAACAGTTCG GACAGGACGG ACCAATCACT GTACACTGCA GTGCTGGGGT GGGCAGGACC 
GGAGTCTTCA TCACCCTCAG CATCGTGCTG GAGAGGATGA GGTATGAAGG AGTTGTCGAC 
CTCTTCCAGA CAGTGAAGAC ACTTCGCACC CAGCGGCCTG CCATGGTGCA GACCGAGGAC 
CAGTACCAGT TGTGCTACAG GGCAGCATTG GAGTACCTGG GGAGCTTTGA CCACTATGCA 
ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104931439 ( XM_010746451.3 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010744753.2 Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X10, mRNA.

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CloneID	OLa211354
Clone ID Related Accession (Same CDS sequence)	XM_010746451.3
Accession Version	XM_010746451.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5448bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	receptor-type tyrosine-protein phosphatase F isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040012.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_010746451.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTGGACATC 
AGCAGCAGCA ACGGGCGCAT TAAACAGCTT CGTTCAGGAG CTCTGCAAAT TGAGAACAGC 
GAGGAGTCCG ACCAAGGGAA ATACGAGTGT GTTGCCATGA ACAGCGCTGG GACCCGTTAC 
TCCGCTCCTG CCAATCTCTA CGTACGAGTG CGAAGAGTCC CACCCAGATT CTCCATTCCG 
CCCACCAACC AAGAGGTGAT GCCAGGCGGC AGCGTCAACC TGACATGCGT GGCAGTGGGC 
TCGCCGATGC CCTACGTCAA GTGGACGATG GGCCTGGTGG AGCTGACCAA GGAGGAGGAG 
ATGCCGATGG GACGCAACGT GCTGGAGGTC AACAACATCC GCGAGTCGGC GAACTACACC 
TGCGTGGCCA TCTCCTCTCT GGGCATGATA GAAGCCACCG CTCAGGTCAC CGTCAAAGCT 
TTGCCAAAGC CTCCCGCCTC CCTCATTGTG ACTGAGACCA CAGCCACCAG TGTGACTCTC 
ACCTGGGACT CTGGAAACCC AGAACCTGTC TCCTACTACA TGATCCAATA CAGAGCCAAG 
ACCTCGGACA ACGGCTTCCA GGAAGTGGAA GGTGTGGCAA CTACCCGTTA CAGCATCGGT 
GGACTTAGCC CCTACTCTGA GTATGAGTTT CGTGTCATGG CTGTCAACAA CATCGGCCGG 
GGGCCACCGA GCGACCCCGT GGAGACACGC ACTGGTGAAC AGGCCCCCTC CTCGCCACCT 
CTGCACGTCC AAGCGCGCAT GCTGAGTGCC AGCACAATGC TGGTCCAGTG GGAACCGCCC 
GAGGAACCCA ACGGGCAAAT CAGGGGCTAC CGGGTCTACT ACAGCTCGGA CTTGACGGCT 
CCGCTCAGCG CCTGGCAGAA GCACAACACA GACGACAGCA GCCTGACGAC CATCTCGGGC 
CTGACTCCTG ATATCACCTA CAGCCTCAGG GTGCTGGGCT TCACCTCTGT AGGTGACGGG 
CCACCTTCTG ACATCCTGCA AGTCAAAACC CAACAGGGAG TCCCTGCCCA GCCGTCCAGC 
TTTGAGGCCG AAGCTGAGCT GGACAGTCGC ATCATGCTAA CATGGCTGTG GCCCGTCCAG 
GACCCCATCA CTAAGTATGA GCTGCAGTAC TGGGAGGCCG GCTCAGACAA CAAGTTACAT 
GTGAGCTTCA ACCCAGCGGG CTCCTACGCT GTGGACGGTC TAAAGCCTGA CACCTTGTAC 
CGTTTCTCTC TGGCAGCCCG TTCTGAGATG GGCTTAGGTG TCTACACCCA ACCCATCGAG 
GCTCGCACCG CCCAGTCCAT GCCGGGAGCA CCCCCCAGGA AGCTGGAGGT TGAGGCCATA 
AACTCCACAG CAATCCGGGT CACCTGGAAG CCGCCCCTGC AGGGGAAGCA GCACGGCCAA 
ATCCGGGGCT ATCAAGTCAT CTTCTCTCGG CTTGAAAACG GCGAACCACG AGGCCAGCCT 
AACATCATGG ACGTTGCTTT GCCAGAGGCA CAGTGGAATA TTGAGGAGTC CACCGAGCAT 
GAGGCCATTA TTGCCGGACT GCACTCTGAA ACCACCTACT CGGTCACCGT GGCAGCATAC 
ACCACCAAAG GAGACGGAGC GCGCAGCAAA GCCAAAGTCA TCACCACGAC GGGTGCAGTG 
CCAGGAAAGC CCACCATGAT GATCAGCACC ACTATGGGCA ACACAGCGCT GATTCAGTGG 
CAGCCGCCTA AGGAAATGGT TGGGGAGCTG ATGGGCTACC AGCTGCAGTA CAAGCGCACT 
GACGAGGAAA ACTACACCTC CCGCGAGCTG AGAAAGATCG ATGACCATTA CACCGTCACC 
GGGCTGCACA AAGGTGCCAC CTATGTCTTC CGCCTCAGTG CCCGCAACCG CGCGGGCATC 
GGCGAAGCAT ACGTGAAAGA GATCAGCACC CCTGAAGACG TGCCGTCGGG CTTCCCGTTC 
AACCTGCAGG TCACGGGACT GACTCAGTCC AGCACCGAGC TGACCTGGGA CCCTCCGCTG 
GTGGCCGAAA GGAACGGCAA GATCGTGCAC TATGTGGTCG TTTACAGGGA CATCAACAGT 
CAGCAGAACA GCACCAACCG GACGAGCGAA ACGCACATGA CCATCCAGGG CCTTAATCCC 
GACACCACCT ACGATATCCG GGTGCAGGCG TTCACCAGTA AGGGCGGAGG ACCCCTCAGC 
CCTAGCATCC AGAGCAGGAC CATGTCGAAC GATTTTCCCA CCCTCAACTT TGGTGTCAAA 
GCCGTCATGA AAACATCTGT CCTCCTCACC TGGGATTTGC CACCGAACTA CAAATCCCAA 
GTGCCTTTCA AGATCCTGTA CAACCAGCAA AGCGTGGAAG TGCAGGGATC CCTGAAGAGG 
AGGCTGATCA CGCAGCTGCA GCCAGACACG GACTACTCTT TTGTGCTGAC AAGCAGGGGC 
AACATTGCTG GTGGTCTACA GCAGCAGGTG TCCATCCGCA CCGCTCCCGA CCTGATCAAC 
GCCAAGCCCA GCACACATCA GCCTCAGGGT AGCAAGATGA CAATCAACCT GCCCAAGGTC 
CAGACCACCA CGCGTGTCAG GTGGTACTAC ATCGTGGTGG TGCCGGTGTC CCAGTCAACG 
CAGGGGTGGA AGAATCCAGA CGAGATGGAC CTGGACGAGC TGTTCAAGTC AAGTGAAGGC 
CCCGTCCTGA GGCGCAGACG TCACCTGGAT TCCTCCAAAC CTTATATCGC TGCCAAGTTG 
GCCTCGTTGC CGACCACCTT CACCCTGGGT GATGAGAAGA GATACAACGG CTTCTACAAC 
AAGCCCCTGA GCAGTCCGCA GGAGTATCTC TGCTTTGTCC TGGCAGTACT CCGATCCGAA 
GGGGCAGACA GCACTACTAA TTATATGACA TTTTCAGCCA GTCCCTACTC AGACCCGGTC 
CACGTGCGAA ACAGCATGGC TCAGGGGATG GAGCAGCCTG AAATGTTGTG GGTTATGGGC 
CCTGTTTTGG CCGTGGTCCT GATTATCATC ATCGTCATCG CCATCCTGCT CTTCAAGAAC 
AAACAGGAAA GGAAGCGAGC GTCGCCCCTA CCCAAAGATG AGCACTCGGG CGGGGTGAAG 
GACTCCCTGC TGGCCAACTC CTCCGACCCT GTGGAGATGA GAAGACTTAA CTACCAGACC 
CCAGGTCCCA GCTCTCATCG CTGCCCCAAC ACTCCAAGAA TGAGGGAGCA CCCGCCCATC 
CCTGTCGTCG ACCTGGCTGA CCACATAGAG CGTCTGAAGG CCAACGACGG CCTACGCTTT 
TCCCAGGAGT ACGAGTCGAT TGATCCAGGG CAGCAGTTTA CCTGGGAGAA CTCCAACATG 
GAAGTCAACA AGCCAAAGAA TCGCTATGCA AACGTCATCG CCTATGACCA CTCCAGAGTG 
GTTCTCACCT CTGTTGACGC CGTTCCAGGC AGTGACTACA TCAACGCCAA CTACATCGAC 
GGCTACAGGA AGCAGAACGC CTACATCGCC ACGCAGGGGC CACTTCCAGA GACACTGAGC 
GACTTCTGGA GGATGGTGTG GGAGCAAAGA TCCAACACCA TCGTCATGAT GACCAGACTG 
GAGGAGAAGT CGCGGGTGAA GTGTGACCAG TACTGGCCGT CGCGAGGAAC GGAGACCTAT 
GGGATGATCC AGGTCACCAT GCTGGACACC GTGGAGTTGG CCACTTACAG CGTCCGTACC 
TTTGCCCTCT ATAAGAATGG CTCCAGTGAG AAGAGAGAGG TGAGACAGTT CCAGTTCATG 
GCCTGGCCAG ACCACGGGGT GCCAGAGTAC CCCACCCCAA TCCTGGCCTT CCTACGGCGG 
GTAAAGGCCT GCAATCCTCC AGACGCAGGG CCTATGGTGG TCCACTGCAG CGCGGGTGTG 
GGCAGGACCG GATGCTTCAT CGTGATAGAT GCCATGCTGG AGCGCATGAA GCACGAGAAG 
TCCGTAGACA TCTACGGCCA CGTGACGTGC ATGCGCGCCC AGAGGAACTA CATGGTCCAG 
ACCGAGGACC AGTACATCTT CATTCACGAA GCCCTGCTGG AGGCCGCCAC GTGCGGCAAC 
ACCGAGGTGC CGGCGCGCAA CCTGTACGCC CACATCCAGA AACTCACCCA GCCCCCGCCC 
GGCGAGACGG TTACTGCCAT GGAGCTGGAA TTCAAGAGAC TGGCCAACTC CAAAGCCCAC 
ACATCCCGCT TCATCAGCGC CAACTTGCCC TGCAACAAGT TCAAGAACCG CCTGGTCAAC 
ATCATGCCTT TCGAGTCCAC CCGCGTCTGC CTGCAGCCCA TCCGTGGCGT CGAGGGCTCC 
GACTACATCA ATGCCAGCTG CATCGACGGC TACAGACAGC AGAAGGCCTA CCTGGCCACG 
CAGGGCCCAC TGGCAGAGAC CACCGAGGAC TTCTGGAGGA TGCTGTGGGA GCACAACTCC 
ACCATCGTCG TCATGCTCAC CAAGCTACGA GAGATGGGCC GGGAGAAGTG CCATCAGTAC 
TGGCCAGCAG AGCGTTCAGC CAGGTACCAG TACTTTGTGG TGGATCCAAT GGCTGAGTAC 
AACATGCCCC AGTACATCCT CAGAGAGTTC AAAGTCACAG ATGCCAGGGA CGGCCAGTCC 
AGAACAATCC GCCAATTCCA GTTCACTGAC TGGCCCGAGC AAGGAGTGCC AAAAACCGGA 
GAGGGCTTCA TTGATTTTAT CGGACAAGTG CACAAAACCA AGGAACAGTT CGGACAGGAC 
GGACCAATCA CTGTACACTG CAGTGCTGGG GTGGGCAGGA CCGGAGTCTT CATCACCCTC 
AGCATCGTGC TGGAGAGGAT GAGGTATGAA GGAGTTGTCG ACCTCTTCCA GACAGTGAAG 
ACACTTCGCA CCCAGCGGCC TGCCATGGTG CAGACCGAGG ACCAGTACCA GTTGTGCTAC 
AGGGCAGCAT TGGAGTACCT GGGGAGCTTT GACCACTATG CAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010744753.2
CDS	479..5926
Translation	MVPNIHLAVPFLPRGALLFLILLSCVVFSSLADSMPSFVKSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVEKTRTATMLCAASGNPDPEISWFKDMLPVDISSSNGRIKQLRSGALQIENSEESDQGKYECVAMNSAGTRYSAPANLYVRVRRVPPRFSIPPTNQEVMPGGSVNLTCVAVGSPMPYVKWTMGLVELTKEEEMPMGRNVLEVNNIRESANYTCVAISSLGMIEATAQVTVKALPKPPASLIVTETTATSVTLTWDSGNPEPVSYYMIQYRAKTSDNGFQEVEGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSDPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRVYYSSDLTAPLSAWQKHNTDDSSLTTISGLTPDITYSLRVLGFTSVGDGPPSDILQVKTQQGVPAQPSSFEAEAELDSRIMLTWLWPVQDPITKYELQYWEAGSDNKLHVSFNPAGSYAVDGLKPDTLYRFSLAARSEMGLGVYTQPIEARTAQSMPGAPPRKLEVEAINSTAIRVTWKPPLQGKQHGQIRGYQVIFSRLENGEPRGQPNIMDVALPEAQWNIEESTEHEAIIAGLHSETTYSVTVAAYTTKGDGARSKAKVITTTGAVPGKPTMMISTTMGNTALIQWQPPKEMVGELMGYQLQYKRTDEENYTSRELRKIDDHYTVTGLHKGATYVFRLSARNRAGIGEAYVKEISTPEDVPSGFPFNLQVTGLTQSSTELTWDPPLVAERNGKIVHYVVVYRDINSQQNSTNRTSETHMTIQGLNPDTTYDIRVQAFTSKGGGPLSPSIQSRTMSNDFPTLNFGVKAVMKTSVLLTWDLPPNYKSQVPFKILYNQQSVEVQGSLKRRLITQLQPDTDYSFVLTSRGNIAGGLQQQVSIRTAPDLINAKPSTHQPQGSKMTINLPKVQTTTRVRWYYIVVVPVSQSTQGWKNPDEMDLDELFKSSEGPVLRRRRHLDSSKPYIAAKLASLPTTFTLGDEKRYNGFYNKPLSSPQEYLCFVLAVLRSEGADSTTNYMTFSASPYSDPVHVRNSMAQGMEQPEMLWVMGPVLAVVLIIIIVIAILLFKNKQERKRASPLPKDEHSGGVKDSLLANSSDPVEMRRLNYQTPGPSSHRCPNTPRMREHPPIPVVDLADHIERLKANDGLRFSQEYESIDPGQQFTWENSNMEVNKPKNRYANVIAYDHSRVVLTSVDAVPGSDYINANYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRSNTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVTMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRAQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPPPGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYLATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_010746451.3
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010746451.3

ORF Insert Sequence:

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTGGACATC 
AGCAGCAGCA ACGGGCGCAT TAAACAGCTT CGTTCAGGAG CTCTGCAAAT TGAGAACAGC 
GAGGAGTCCG ACCAAGGGAA ATACGAGTGT GTTGCCATGA ACAGCGCTGG GACCCGTTAC 
TCCGCTCCTG CCAATCTCTA CGTACGAGTG CGAAGAGTCC CACCCAGATT CTCCATTCCG 
CCCACCAACC AAGAGGTGAT GCCAGGCGGC AGCGTCAACC TGACATGCGT GGCAGTGGGC 
TCGCCGATGC CCTACGTCAA GTGGACGATG GGCCTGGTGG AGCTGACCAA GGAGGAGGAG 
ATGCCGATGG GACGCAACGT GCTGGAGGTC AACAACATCC GCGAGTCGGC GAACTACACC 
TGCGTGGCCA TCTCCTCTCT GGGCATGATA GAAGCCACCG CTCAGGTCAC CGTCAAAGCT 
TTGCCAAAGC CTCCCGCCTC CCTCATTGTG ACTGAGACCA CAGCCACCAG TGTGACTCTC 
ACCTGGGACT CTGGAAACCC AGAACCTGTC TCCTACTACA TGATCCAATA CAGAGCCAAG 
ACCTCGGACA ACGGCTTCCA GGAAGTGGAA GGTGTGGCAA CTACCCGTTA CAGCATCGGT 
GGACTTAGCC CCTACTCTGA GTATGAGTTT CGTGTCATGG CTGTCAACAA CATCGGCCGG 
GGGCCACCGA GCGACCCCGT GGAGACACGC ACTGGTGAAC AGGCCCCCTC CTCGCCACCT 
CTGCACGTCC AAGCGCGCAT GCTGAGTGCC AGCACAATGC TGGTCCAGTG GGAACCGCCC 
GAGGAACCCA ACGGGCAAAT CAGGGGCTAC CGGGTCTACT ACAGCTCGGA CTTGACGGCT 
CCGCTCAGCG CCTGGCAGAA GCACAACACA GACGACAGCA GCCTGACGAC CATCTCGGGC 
CTGACTCCTG ATATCACCTA CAGCCTCAGG GTGCTGGGCT TCACCTCTGT AGGTGACGGG 
CCACCTTCTG ACATCCTGCA AGTCAAAACC CAACAGGGAG TCCCTGCCCA GCCGTCCAGC 
TTTGAGGCCG AAGCTGAGCT GGACAGTCGC ATCATGCTAA CATGGCTGTG GCCCGTCCAG 
GACCCCATCA CTAAGTATGA GCTGCAGTAC TGGGAGGCCG GCTCAGACAA CAAGTTACAT 
GTGAGCTTCA ACCCAGCGGG CTCCTACGCT GTGGACGGTC TAAAGCCTGA CACCTTGTAC 
CGTTTCTCTC TGGCAGCCCG TTCTGAGATG GGCTTAGGTG TCTACACCCA ACCCATCGAG 
GCTCGCACCG CCCAGTCCAT GCCGGGAGCA CCCCCCAGGA AGCTGGAGGT TGAGGCCATA 
AACTCCACAG CAATCCGGGT CACCTGGAAG CCGCCCCTGC AGGGGAAGCA GCACGGCCAA 
ATCCGGGGCT ATCAAGTCAT CTTCTCTCGG CTTGAAAACG GCGAACCACG AGGCCAGCCT 
AACATCATGG ACGTTGCTTT GCCAGAGGCA CAGTGGAATA TTGAGGAGTC CACCGAGCAT 
GAGGCCATTA TTGCCGGACT GCACTCTGAA ACCACCTACT CGGTCACCGT GGCAGCATAC 
ACCACCAAAG GAGACGGAGC GCGCAGCAAA GCCAAAGTCA TCACCACGAC GGGTGCAGTG 
CCAGGAAAGC CCACCATGAT GATCAGCACC ACTATGGGCA ACACAGCGCT GATTCAGTGG 
CAGCCGCCTA AGGAAATGGT TGGGGAGCTG ATGGGCTACC AGCTGCAGTA CAAGCGCACT 
GACGAGGAAA ACTACACCTC CCGCGAGCTG AGAAAGATCG ATGACCATTA CACCGTCACC 
GGGCTGCACA AAGGTGCCAC CTATGTCTTC CGCCTCAGTG CCCGCAACCG CGCGGGCATC 
GGCGAAGCAT ACGTGAAAGA GATCAGCACC CCTGAAGACG TGCCGTCGGG CTTCCCGTTC 
AACCTGCAGG TCACGGGACT GACTCAGTCC AGCACCGAGC TGACCTGGGA CCCTCCGCTG 
GTGGCCGAAA GGAACGGCAA GATCGTGCAC TATGTGGTCG TTTACAGGGA CATCAACAGT 
CAGCAGAACA GCACCAACCG GACGAGCGAA ACGCACATGA CCATCCAGGG CCTTAATCCC 
GACACCACCT ACGATATCCG GGTGCAGGCG TTCACCAGTA AGGGCGGAGG ACCCCTCAGC 
CCTAGCATCC AGAGCAGGAC CATGTCGAAC GATTTTCCCA CCCTCAACTT TGGTGTCAAA 
GCCGTCATGA AAACATCTGT CCTCCTCACC TGGGATTTGC CACCGAACTA CAAATCCCAA 
GTGCCTTTCA AGATCCTGTA CAACCAGCAA AGCGTGGAAG TGCAGGGATC CCTGAAGAGG 
AGGCTGATCA CGCAGCTGCA GCCAGACACG GACTACTCTT TTGTGCTGAC AAGCAGGGGC 
AACATTGCTG GTGGTCTACA GCAGCAGGTG TCCATCCGCA CCGCTCCCGA CCTGATCAAC 
GCCAAGCCCA GCACACATCA GCCTCAGGGT AGCAAGATGA CAATCAACCT GCCCAAGGTC 
CAGACCACCA CGCGTGTCAG GTGGTACTAC ATCGTGGTGG TGCCGGTGTC CCAGTCAACG 
CAGGGGTGGA AGAATCCAGA CGAGATGGAC CTGGACGAGC TGTTCAAGTC AAGTGAAGGC 
CCCGTCCTGA GGCGCAGACG TCACCTGGAT TCCTCCAAAC CTTATATCGC TGCCAAGTTG 
GCCTCGTTGC CGACCACCTT CACCCTGGGT GATGAGAAGA GATACAACGG CTTCTACAAC 
AAGCCCCTGA GCAGTCCGCA GGAGTATCTC TGCTTTGTCC TGGCAGTACT CCGATCCGAA 
GGGGCAGACA GCACTACTAA TTATATGACA TTTTCAGCCA GTCCCTACTC AGACCCGGTC 
CACGTGCGAA ACAGCATGGC TCAGGGGATG GAGCAGCCTG AAATGTTGTG GGTTATGGGC 
CCTGTTTTGG CCGTGGTCCT GATTATCATC ATCGTCATCG CCATCCTGCT CTTCAAGAAC 
AAACAGGAAA GGAAGCGAGC GTCGCCCCTA CCCAAAGATG AGCACTCGGG CGGGGTGAAG 
GACTCCCTGC TGGCCAACTC CTCCGACCCT GTGGAGATGA GAAGACTTAA CTACCAGACC 
CCAGGTCCCA GCTCTCATCG CTGCCCCAAC ACTCCAAGAA TGAGGGAGCA CCCGCCCATC 
CCTGTCGTCG ACCTGGCTGA CCACATAGAG CGTCTGAAGG CCAACGACGG CCTACGCTTT 
TCCCAGGAGT ACGAGTCGAT TGATCCAGGG CAGCAGTTTA CCTGGGAGAA CTCCAACATG 
GAAGTCAACA AGCCAAAGAA TCGCTATGCA AACGTCATCG CCTATGACCA CTCCAGAGTG 
GTTCTCACCT CTGTTGACGC CGTTCCAGGC AGTGACTACA TCAACGCCAA CTACATCGAC 
GGCTACAGGA AGCAGAACGC CTACATCGCC ACGCAGGGGC CACTTCCAGA GACACTGAGC 
GACTTCTGGA GGATGGTGTG GGAGCAAAGA TCCAACACCA TCGTCATGAT GACCAGACTG 
GAGGAGAAGT CGCGGGTGAA GTGTGACCAG TACTGGCCGT CGCGAGGAAC GGAGACCTAT 
GGGATGATCC AGGTCACCAT GCTGGACACC GTGGAGTTGG CCACTTACAG CGTCCGTACC 
TTTGCCCTCT ATAAGAATGG CTCCAGTGAG AAGAGAGAGG TGAGACAGTT CCAGTTCATG 
GCCTGGCCAG ACCACGGGGT GCCAGAGTAC CCCACCCCAA TCCTGGCCTT CCTACGGCGG 
GTAAAGGCCT GCAATCCTCC AGACGCAGGG CCTATGGTGG TCCACTGCAG CGCGGGTGTG 
GGCAGGACCG GATGCTTCAT CGTGATAGAT GCCATGCTGG AGCGCATGAA GCACGAGAAG 
TCCGTAGACA TCTACGGCCA CGTGACGTGC ATGCGCGCCC AGAGGAACTA CATGGTCCAG 
ACCGAGGACC AGTACATCTT CATTCACGAA GCCCTGCTGG AGGCCGCCAC GTGCGGCAAC 
ACCGAGGTGC CGGCGCGCAA CCTGTACGCC CACATCCAGA AACTCACCCA GCCCCCGCCC 
GGCGAGACGG TTACTGCCAT GGAGCTGGAA TTCAAGAGAC TGGCCAACTC CAAAGCCCAC 
ACATCCCGCT TCATCAGCGC CAACTTGCCC TGCAACAAGT TCAAGAACCG CCTGGTCAAC 
ATCATGCCTT TCGAGTCCAC CCGCGTCTGC CTGCAGCCCA TCCGTGGCGT CGAGGGCTCC 
GACTACATCA ATGCCAGCTG CATCGACGGC TACAGACAGC AGAAGGCCTA CCTGGCCACG 
CAGGGCCCAC TGGCAGAGAC CACCGAGGAC TTCTGGAGGA TGCTGTGGGA GCACAACTCC 
ACCATCGTCG TCATGCTCAC CAAGCTACGA GAGATGGGCC GGGAGAAGTG CCATCAGTAC 
TGGCCAGCAG AGCGTTCAGC CAGGTACCAG TACTTTGTGG TGGATCCAAT GGCTGAGTAC 
AACATGCCCC AGTACATCCT CAGAGAGTTC AAAGTCACAG ATGCCAGGGA CGGCCAGTCC 
AGAACAATCC GCCAATTCCA GTTCACTGAC TGGCCCGAGC AAGGAGTGCC AAAAACCGGA 
GAGGGCTTCA TTGATTTTAT CGGACAAGTG CACAAAACCA AGGAACAGTT CGGACAGGAC 
GGACCAATCA CTGTACACTG CAGTGCTGGG GTGGGCAGGA CCGGAGTCTT CATCACCCTC 
AGCATCGTGC TGGAGAGGAT GAGGTATGAA GGAGTTGTCG ACCTCTTCCA GACAGTGAAG 
ACACTTCGCA CCCAGCGGCC TGCCATGGTG CAGACCGAGG ACCAGTACCA GTTGTGCTAC 
AGGGCAGCAT TGGAGTACCT GGGGAGCTTT GACCACTATG CAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104931439 ( XM_010746452.3 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010744754.2 Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X11, mRNA.

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CloneID	OLa211355
Clone ID Related Accession (Same CDS sequence)	XM_010746452.3
Accession Version	XM_010746452.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	receptor-type tyrosine-protein phosphatase F isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040012.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_010746452.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTGGACATC 
AGCAGCAGCA ACGGGCGCAT TAAACAGCTT CGTTCAGGAG CTCTGCAAAT TGAGAACAGC 
GAGGAGTCCG ACCAAGGGAA ATACGAGTGT GTTGCCATGA ACAGCGCTGG GACCCGTTAC 
TCCGCTCCTG CCAATCTCTA CGTACGAGTG CGAAGAGTCC CACCCAGATT CTCCATTCCG 
CCCACCAACC AAGAGGTGAT GCCAGGCGGC AGCGTCAACC TGACATGCGT GGCAGTGGGC 
TCGCCGATGC CCTACGTCAA GTGGACGATG GGCCTGGTGG AGCTGACCAA GGAGGAGGAG 
ATGCCGATGG GACGCAACGT GCTGGAGGTC AACAACATCC GCGAGTCGGC GAACTACACC 
TGCGTGGCCA TCTCCTCTCT GGGCATGATA GAAGCCACCG CTCAGGTCAC CGTCAAAGCT 
TTGCCAAAGC CTCCCGCCTC CCTCATTGTG ACTGAGACCA CAGCCACCAG TGTGACTCTC 
ACCTGGGACT CTGGAAACCC AGAACCTGTC TCCTACTACA TGATCCAATA CAGAGCCAAG 
ACCTCGGACA ACGGCTTCCA GGAAGTGGAA GGTGTGGCAA CTACCCGTTA CAGCATCGGT 
GGACTTAGCC CCTACTCTGA GTATGAGTTT CGTGTCATGG CTGTCAACAA CATCGGCCGG 
GGGCCACCGA GCGACCCCGT GGAGACACGC ACTGGTGAAC AGGCCCCCTC CTCGCCACCT 
CTGCACGTCC AAGCGCGCAT GCTGAGTGCC AGCACAATGC TGGTCCAGTG GGAACCGCCC 
GAGGAACCCA ACGGGCAAAT CAGGGGCTAC CGGGTCTACT ACAGCTCGGA CTTGACGGCT 
CCGCTCAGCG CCTGGCAGAA GCACAACACA GACGACAGCA GCCTGACGAC CATCTCGGGC 
CTGACTCCTG ATATCACCTA CAGCCTCAGG GTGCTGGGCT TCACCTCTGT AGGTGACGGG 
CCACCTTCTG ACATCCTGCA AGTCAAAACC CAACAGGGAG TCCCTGCCCA GCCGTCCAGC 
TTTGAGGCCG AAGCTGAGCT GGACAGTCGC ATCATGCTAA CATGGCTGTG GCCCGTCCAG 
GACCCCATCA CTAAGTATGA GCTGCAGTAC TGGGAGGCCG GCTCAGACAA CAAGTTACAT 
GTGAGCTTCA ACCCAGCGGG CTCCTACGCT GTGGACGGTC TAAAGCCTGA CACCTTGTAC 
CGTTTCTCTC TGGCAGCCCG TTCTGAGATG GGCTTAGGTG TCTACACCCA ACCCATCGAG 
GCTCGCACCG CCCAGTCCAT GCCGGGAGCA CCCCCCAGGA AGCTGGAGGT TGAGGCCATA 
AACTCCACAG CAATCCGGGT CACCTGGAAG CCGCCCCTGC AGGGGAAGCA GCACGGCCAA 
ATCCGGGGCT ATCAAGTCAT CTTCTCTCGG CTTGAAAACG GCGAACCACG AGGCCAGCCT 
AACATCATGG ACGTTGCTTT GCCAGAGGCA CAGGAGGCCA TTATTGCCGG ACTGCACTCT 
GAAACCACCT ACTCGGTCAC CGTGGCAGCA TACACCACCA AAGGAGACGG AGCGCGCAGC 
AAAGCCAAAG TCATCACCAC GACGGGTGCA GTGCCAGGAA AGCCCACCAT GATGATCAGC 
ACCACTATGG GCAACACAGC GCTGATTCAG TGGCAGCCGC CTAAGGAAAT GGTTGGGGAG 
CTGATGGGCT ACCAGCTGCA GTACAAGCGC ACTGACGAGG AAAACTACAC CTCCCGCGAG 
CTGAGAAAGA TCGATGACCA TTACACCGTC ACCGGGCTGC ACAAAGGTGC CACCTATGTC 
TTCCGCCTCA GTGCCCGCAA CCGCGCGGGC ATCGGCGAAG CATACGTGAA AGAGATCAGC 
ACCCCTGAAG ACGTGCCGTC GGGCTTCCCG TTCAACCTGC AGGTCACGGG ACTGACTCAG 
TCCAGCACCG AGCTGACCTG GGACCCTCCG CTGGTGGCCG AAAGGAACGG CAAGATCGTG 
CACTATGTGG TCGTTTACAG GGACATCAAC AGTCAGCAGA ACAGCACCAA CCGGACGAGC 
GAAACGCACA TGACCATCCA GGGCCTTAAT CCCGACACCA CCTACGATAT CCGGGTGCAG 
GCGTTCACCA GTAAGGGCGG AGGACCCCTC AGCCCTAGCA TCCAGAGCAG GACCATGTCG 
AACGATTTTC CCACCCTCAA CTTTGGTGTC AAAGCCGTCA TGAAAACATC TGTCCTCCTC 
ACCTGGGATT TGCCACCGAA CTACAAATCC CAAGTGCCTT TCAAGATCCT GTACAACCAG 
CAAAGCGTGG AAGTGCAGGG ATCCCTGAAG AGGAGGCTGA TCACGCAGCT GCAGCCAGAC 
ACGGACTACT CTTTTGTGCT GACAAGCAGG GGCAACATTG CTGGTGGTCT ACAGCAGCAG 
GTGTCCATCC GCACCGCTCC CGACCTGATC AACGCCAAGC CCAGCACACA TCAGCCTCAG 
GGTAGCAAGA TGACAATCAA CCTGCCCAAG GTCCAGACCA CCACGCGTGT CAGGTGGTAC 
TACATCGTGG TGGTGCCGGT GTCCCAGTCA ACGCAGGGGT GGAAGAATCC AGACGAGATG 
GACCTGGACG AGCTGTTCAA GTCAAGTGAA GGCCCCGTCC TGAGGCGCAG ACGTCACCTG 
GATTCCTCCA AACCTTATAT CGCTGCCAAG TTGGCCTCGT TGCCGACCAC CTTCACCCTG 
GGTGATGAGA AGAGATACAA CGGCTTCTAC AACAAGCCCC TGAGCAGTCC GCAGGAGTAT 
CTCTGCTTTG TCCTGGCAGT ACTCCGATCC GAAGGGGCAG ACAGCACTAC TAATTATATG 
ACATTTTCAG CCAGTCCCTA CTCAGACCCG GTCCACGTGC GAAACAGCAT GGCTCAGGGG 
ATGGAGCAGC CTGAAATGTT GTGGGTTATG GGCCCTGTTT TGGCCGTGGT CCTGATTATC 
ATCATCGTCA TCGCCATCCT GCTCTTCAAG AACAAACAGG AAAGGAAGCG AGCGTCGCCC 
CTACCCAAAG ATGAGCACTC GGGCGGGGTG AAGGACTCCC TGCTGGCCAA CTCCTCCGAC 
CCTGTGGAGA TGAGAAGACT TAACTACCAG ACCCCAGGTC CCAGCTCTCA TCGCTGCCCC 
AACACTCCAA GAATGAGGGA GCACCCGCCC ATCCCTGTCG TCGACCTGGC TGACCACATA 
GAGCGTCTGA AGGCCAACGA CGGCCTACGC TTTTCCCAGG AGTACGAGTC GATTGATCCA 
GGGCAGCAGT TTACCTGGGA GAACTCCAAC ATGGAAGTCA ACAAGCCAAA GAATCGCTAT 
GCAAACGTCA TCGCCTATGA CCACTCCAGA GTGGTTCTCA CCTCTGTTGA CGCCGTTCCA 
GGCAGTGACT ACATCAACGC CAACTACATC GACGGCTACA GGAAGCAGAA CGCCTACATC 
GCCACGCAGG GGCCACTTCC AGAGACACTG AGCGACTTCT GGAGGATGGT GTGGGAGCAA 
AGATCCAACA CCATCGTCAT GATGACCAGA CTGGAGGAGA AGTCGCGGGT GAAGTGTGAC 
CAGTACTGGC CGTCGCGAGG AACGGAGACC TATGGGATGA TCCAGGTCAC CATGCTGGAC 
ACCGTGGAGT TGGCCACTTA CAGCGTCCGT ACCTTTGCCC TCTATAAGAA TGGCTCCAGT 
GAGAAGAGAG AGGTGAGACA GTTCCAGTTC ATGGCCTGGC CAGACCACGG GGTGCCAGAG 
TACCCCACCC CAATCCTGGC CTTCCTACGG CGGGTAAAGG CCTGCAATCC TCCAGACGCA 
GGGCCTATGG TGGTCCACTG CAGCGCGGGT GTGGGCAGGA CCGGATGCTT CATCGTGATA 
GATGCCATGC TGGAGCGCAT GAAGCACGAG AAGTCCGTAG ACATCTACGG CCACGTGACG 
TGCATGCGCG CCCAGAGGAA CTACATGGTC CAGACCGAGG ACCAGTACAT CTTCATTCAC 
GAAGCCCTGC TGGAGGCCGC CACGTGCGGC AACACCGAGG TGCCGGCGCG CAACCTGTAC 
GCCCACATCC AGAAACTCAC CCAGCCCCCG CCCGGCGAGA CGGTTACTGC CATGGAGCTG 
GAATTCAAGA GACTGGCCAA CTCCAAAGCC CACACATCCC GCTTCATCAG CGCCAACTTG 
CCCTGCAACA AGTTCAAGAA CCGCCTGGTC AACATCATGC CTTTCGAGTC CACCCGCGTC 
TGCCTGCAGC CCATCCGTGG CGTCGAGGGC TCCGACTACA TCAATGCCAG CTGCATCGAC 
GGCTACAGAC AGCAGAAGGC CTACCTGGCC ACGCAGGGCC CACTGGCAGA GACCACCGAG 
GACTTCTGGA GGATGCTGTG GGAGCACAAC TCCACCATCG TCGTCATGCT CACCAAGCTA 
CGAGAGATGG GCCGGGAGAA GTGCCATCAG TACTGGCCAG CAGAGCGTTC AGCCAGGTAC 
CAGTACTTTG TGGTGGATCC AATGGCTGAG TACAACATGC CCCAGTACAT CCTCAGAGAG 
TTCAAAGTCA CAGATGCCAG GGACGGCCAG TCCAGAACAA TCCGCCAATT CCAGTTCACT 
GACTGGCCCG AGCAAGGAGT GCCAAAAACC GGAGAGGGCT TCATTGATTT TATCGGACAA 
GTGCACAAAA CCAAGGAACA GTTCGGACAG GACGGACCAA TCACTGTACA CTGCAGTGCT 
GGGGTGGGCA GGACCGGAGT CTTCATCACC CTCAGCATCG TGCTGGAGAG GATGAGGTAT 
GAAGGAGTTG TCGACCTCTT CCAGACAGTG AAGACACTTC GCACCCAGCG GCCTGCCATG 
GTGCAGACCG AGGACCAGTA CCAGTTGTGC TACAGGGCAG CATTGGAGTA CCTGGGGAGC 
TTTGACCACT ATGCAACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010744754.2
CDS	478..5898
Translation	MVPNIHLAVPFLPRGALLFLILLSCVVFSSLADSMPSFVKSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVEKTRTATMLCAASGNPDPEISWFKDMLPVDISSSNGRIKQLRSGALQIENSEESDQGKYECVAMNSAGTRYSAPANLYVRVRRVPPRFSIPPTNQEVMPGGSVNLTCVAVGSPMPYVKWTMGLVELTKEEEMPMGRNVLEVNNIRESANYTCVAISSLGMIEATAQVTVKALPKPPASLIVTETTATSVTLTWDSGNPEPVSYYMIQYRAKTSDNGFQEVEGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSDPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRVYYSSDLTAPLSAWQKHNTDDSSLTTISGLTPDITYSLRVLGFTSVGDGPPSDILQVKTQQGVPAQPSSFEAEAELDSRIMLTWLWPVQDPITKYELQYWEAGSDNKLHVSFNPAGSYAVDGLKPDTLYRFSLAARSEMGLGVYTQPIEARTAQSMPGAPPRKLEVEAINSTAIRVTWKPPLQGKQHGQIRGYQVIFSRLENGEPRGQPNIMDVALPEAQEAIIAGLHSETTYSVTVAAYTTKGDGARSKAKVITTTGAVPGKPTMMISTTMGNTALIQWQPPKEMVGELMGYQLQYKRTDEENYTSRELRKIDDHYTVTGLHKGATYVFRLSARNRAGIGEAYVKEISTPEDVPSGFPFNLQVTGLTQSSTELTWDPPLVAERNGKIVHYVVVYRDINSQQNSTNRTSETHMTIQGLNPDTTYDIRVQAFTSKGGGPLSPSIQSRTMSNDFPTLNFGVKAVMKTSVLLTWDLPPNYKSQVPFKILYNQQSVEVQGSLKRRLITQLQPDTDYSFVLTSRGNIAGGLQQQVSIRTAPDLINAKPSTHQPQGSKMTINLPKVQTTTRVRWYYIVVVPVSQSTQGWKNPDEMDLDELFKSSEGPVLRRRRHLDSSKPYIAAKLASLPTTFTLGDEKRYNGFYNKPLSSPQEYLCFVLAVLRSEGADSTTNYMTFSASPYSDPVHVRNSMAQGMEQPEMLWVMGPVLAVVLIIIIVIAILLFKNKQERKRASPLPKDEHSGGVKDSLLANSSDPVEMRRLNYQTPGPSSHRCPNTPRMREHPPIPVVDLADHIERLKANDGLRFSQEYESIDPGQQFTWENSNMEVNKPKNRYANVIAYDHSRVVLTSVDAVPGSDYINANYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRSNTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVTMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRAQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPPPGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYLATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_010746452.3
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010746452.3

ORF Insert Sequence:

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTGGACATC 
AGCAGCAGCA ACGGGCGCAT TAAACAGCTT CGTTCAGGAG CTCTGCAAAT TGAGAACAGC 
GAGGAGTCCG ACCAAGGGAA ATACGAGTGT GTTGCCATGA ACAGCGCTGG GACCCGTTAC 
TCCGCTCCTG CCAATCTCTA CGTACGAGTG CGAAGAGTCC CACCCAGATT CTCCATTCCG 
CCCACCAACC AAGAGGTGAT GCCAGGCGGC AGCGTCAACC TGACATGCGT GGCAGTGGGC 
TCGCCGATGC CCTACGTCAA GTGGACGATG GGCCTGGTGG AGCTGACCAA GGAGGAGGAG 
ATGCCGATGG GACGCAACGT GCTGGAGGTC AACAACATCC GCGAGTCGGC GAACTACACC 
TGCGTGGCCA TCTCCTCTCT GGGCATGATA GAAGCCACCG CTCAGGTCAC CGTCAAAGCT 
TTGCCAAAGC CTCCCGCCTC CCTCATTGTG ACTGAGACCA CAGCCACCAG TGTGACTCTC 
ACCTGGGACT CTGGAAACCC AGAACCTGTC TCCTACTACA TGATCCAATA CAGAGCCAAG 
ACCTCGGACA ACGGCTTCCA GGAAGTGGAA GGTGTGGCAA CTACCCGTTA CAGCATCGGT 
GGACTTAGCC CCTACTCTGA GTATGAGTTT CGTGTCATGG CTGTCAACAA CATCGGCCGG 
GGGCCACCGA GCGACCCCGT GGAGACACGC ACTGGTGAAC AGGCCCCCTC CTCGCCACCT 
CTGCACGTCC AAGCGCGCAT GCTGAGTGCC AGCACAATGC TGGTCCAGTG GGAACCGCCC 
GAGGAACCCA ACGGGCAAAT CAGGGGCTAC CGGGTCTACT ACAGCTCGGA CTTGACGGCT 
CCGCTCAGCG CCTGGCAGAA GCACAACACA GACGACAGCA GCCTGACGAC CATCTCGGGC 
CTGACTCCTG ATATCACCTA CAGCCTCAGG GTGCTGGGCT TCACCTCTGT AGGTGACGGG 
CCACCTTCTG ACATCCTGCA AGTCAAAACC CAACAGGGAG TCCCTGCCCA GCCGTCCAGC 
TTTGAGGCCG AAGCTGAGCT GGACAGTCGC ATCATGCTAA CATGGCTGTG GCCCGTCCAG 
GACCCCATCA CTAAGTATGA GCTGCAGTAC TGGGAGGCCG GCTCAGACAA CAAGTTACAT 
GTGAGCTTCA ACCCAGCGGG CTCCTACGCT GTGGACGGTC TAAAGCCTGA CACCTTGTAC 
CGTTTCTCTC TGGCAGCCCG TTCTGAGATG GGCTTAGGTG TCTACACCCA ACCCATCGAG 
GCTCGCACCG CCCAGTCCAT GCCGGGAGCA CCCCCCAGGA AGCTGGAGGT TGAGGCCATA 
AACTCCACAG CAATCCGGGT CACCTGGAAG CCGCCCCTGC AGGGGAAGCA GCACGGCCAA 
ATCCGGGGCT ATCAAGTCAT CTTCTCTCGG CTTGAAAACG GCGAACCACG AGGCCAGCCT 
AACATCATGG ACGTTGCTTT GCCAGAGGCA CAGGAGGCCA TTATTGCCGG ACTGCACTCT 
GAAACCACCT ACTCGGTCAC CGTGGCAGCA TACACCACCA AAGGAGACGG AGCGCGCAGC 
AAAGCCAAAG TCATCACCAC GACGGGTGCA GTGCCAGGAA AGCCCACCAT GATGATCAGC 
ACCACTATGG GCAACACAGC GCTGATTCAG TGGCAGCCGC CTAAGGAAAT GGTTGGGGAG 
CTGATGGGCT ACCAGCTGCA GTACAAGCGC ACTGACGAGG AAAACTACAC CTCCCGCGAG 
CTGAGAAAGA TCGATGACCA TTACACCGTC ACCGGGCTGC ACAAAGGTGC CACCTATGTC 
TTCCGCCTCA GTGCCCGCAA CCGCGCGGGC ATCGGCGAAG CATACGTGAA AGAGATCAGC 
ACCCCTGAAG ACGTGCCGTC GGGCTTCCCG TTCAACCTGC AGGTCACGGG ACTGACTCAG 
TCCAGCACCG AGCTGACCTG GGACCCTCCG CTGGTGGCCG AAAGGAACGG CAAGATCGTG 
CACTATGTGG TCGTTTACAG GGACATCAAC AGTCAGCAGA ACAGCACCAA CCGGACGAGC 
GAAACGCACA TGACCATCCA GGGCCTTAAT CCCGACACCA CCTACGATAT CCGGGTGCAG 
GCGTTCACCA GTAAGGGCGG AGGACCCCTC AGCCCTAGCA TCCAGAGCAG GACCATGTCG 
AACGATTTTC CCACCCTCAA CTTTGGTGTC AAAGCCGTCA TGAAAACATC TGTCCTCCTC 
ACCTGGGATT TGCCACCGAA CTACAAATCC CAAGTGCCTT TCAAGATCCT GTACAACCAG 
CAAAGCGTGG AAGTGCAGGG ATCCCTGAAG AGGAGGCTGA TCACGCAGCT GCAGCCAGAC 
ACGGACTACT CTTTTGTGCT GACAAGCAGG GGCAACATTG CTGGTGGTCT ACAGCAGCAG 
GTGTCCATCC GCACCGCTCC CGACCTGATC AACGCCAAGC CCAGCACACA TCAGCCTCAG 
GGTAGCAAGA TGACAATCAA CCTGCCCAAG GTCCAGACCA CCACGCGTGT CAGGTGGTAC 
TACATCGTGG TGGTGCCGGT GTCCCAGTCA ACGCAGGGGT GGAAGAATCC AGACGAGATG 
GACCTGGACG AGCTGTTCAA GTCAAGTGAA GGCCCCGTCC TGAGGCGCAG ACGTCACCTG 
GATTCCTCCA AACCTTATAT CGCTGCCAAG TTGGCCTCGT TGCCGACCAC CTTCACCCTG 
GGTGATGAGA AGAGATACAA CGGCTTCTAC AACAAGCCCC TGAGCAGTCC GCAGGAGTAT 
CTCTGCTTTG TCCTGGCAGT ACTCCGATCC GAAGGGGCAG ACAGCACTAC TAATTATATG 
ACATTTTCAG CCAGTCCCTA CTCAGACCCG GTCCACGTGC GAAACAGCAT GGCTCAGGGG 
ATGGAGCAGC CTGAAATGTT GTGGGTTATG GGCCCTGTTT TGGCCGTGGT CCTGATTATC 
ATCATCGTCA TCGCCATCCT GCTCTTCAAG AACAAACAGG AAAGGAAGCG AGCGTCGCCC 
CTACCCAAAG ATGAGCACTC GGGCGGGGTG AAGGACTCCC TGCTGGCCAA CTCCTCCGAC 
CCTGTGGAGA TGAGAAGACT TAACTACCAG ACCCCAGGTC CCAGCTCTCA TCGCTGCCCC 
AACACTCCAA GAATGAGGGA GCACCCGCCC ATCCCTGTCG TCGACCTGGC TGACCACATA 
GAGCGTCTGA AGGCCAACGA CGGCCTACGC TTTTCCCAGG AGTACGAGTC GATTGATCCA 
GGGCAGCAGT TTACCTGGGA GAACTCCAAC ATGGAAGTCA ACAAGCCAAA GAATCGCTAT 
GCAAACGTCA TCGCCTATGA CCACTCCAGA GTGGTTCTCA CCTCTGTTGA CGCCGTTCCA 
GGCAGTGACT ACATCAACGC CAACTACATC GACGGCTACA GGAAGCAGAA CGCCTACATC 
GCCACGCAGG GGCCACTTCC AGAGACACTG AGCGACTTCT GGAGGATGGT GTGGGAGCAA 
AGATCCAACA CCATCGTCAT GATGACCAGA CTGGAGGAGA AGTCGCGGGT GAAGTGTGAC 
CAGTACTGGC CGTCGCGAGG AACGGAGACC TATGGGATGA TCCAGGTCAC CATGCTGGAC 
ACCGTGGAGT TGGCCACTTA CAGCGTCCGT ACCTTTGCCC TCTATAAGAA TGGCTCCAGT 
GAGAAGAGAG AGGTGAGACA GTTCCAGTTC ATGGCCTGGC CAGACCACGG GGTGCCAGAG 
TACCCCACCC CAATCCTGGC CTTCCTACGG CGGGTAAAGG CCTGCAATCC TCCAGACGCA 
GGGCCTATGG TGGTCCACTG CAGCGCGGGT GTGGGCAGGA CCGGATGCTT CATCGTGATA 
GATGCCATGC TGGAGCGCAT GAAGCACGAG AAGTCCGTAG ACATCTACGG CCACGTGACG 
TGCATGCGCG CCCAGAGGAA CTACATGGTC CAGACCGAGG ACCAGTACAT CTTCATTCAC 
GAAGCCCTGC TGGAGGCCGC CACGTGCGGC AACACCGAGG TGCCGGCGCG CAACCTGTAC 
GCCCACATCC AGAAACTCAC CCAGCCCCCG CCCGGCGAGA CGGTTACTGC CATGGAGCTG 
GAATTCAAGA GACTGGCCAA CTCCAAAGCC CACACATCCC GCTTCATCAG CGCCAACTTG 
CCCTGCAACA AGTTCAAGAA CCGCCTGGTC AACATCATGC CTTTCGAGTC CACCCGCGTC 
TGCCTGCAGC CCATCCGTGG CGTCGAGGGC TCCGACTACA TCAATGCCAG CTGCATCGAC 
GGCTACAGAC AGCAGAAGGC CTACCTGGCC ACGCAGGGCC CACTGGCAGA GACCACCGAG 
GACTTCTGGA GGATGCTGTG GGAGCACAAC TCCACCATCG TCGTCATGCT CACCAAGCTA 
CGAGAGATGG GCCGGGAGAA GTGCCATCAG TACTGGCCAG CAGAGCGTTC AGCCAGGTAC 
CAGTACTTTG TGGTGGATCC AATGGCTGAG TACAACATGC CCCAGTACAT CCTCAGAGAG 
TTCAAAGTCA CAGATGCCAG GGACGGCCAG TCCAGAACAA TCCGCCAATT CCAGTTCACT 
GACTGGCCCG AGCAAGGAGT GCCAAAAACC GGAGAGGGCT TCATTGATTT TATCGGACAA 
GTGCACAAAA CCAAGGAACA GTTCGGACAG GACGGACCAA TCACTGTACA CTGCAGTGCT 
GGGGTGGGCA GGACCGGAGT CTTCATCACC CTCAGCATCG TGCTGGAGAG GATGAGGTAT 
GAAGGAGTTG TCGACCTCTT CCAGACAGTG AAGACACTTC GCACCCAGCG GCCTGCCATG 
GTGCAGACCG AGGACCAGTA CCAGTTGTGC TACAGGGCAG CATTGGAGTA CCTGGGGAGC 
TTTGACCACT ATGCAACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104931439 ( XM_010746450.3 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010744752.2 Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X12, mRNA.

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CloneID	OLa211356
Clone ID Related Accession (Same CDS sequence)	XM_010746450.3
Accession Version	XM_010746450.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5709bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	receptor-type tyrosine-protein phosphatase F isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040012.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_010746450.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTGGACATC 
AGCAGCAGCA ACGGGCGCAT TAAACAGCTT CGTTCAGGAG CTCTGCAAAT TGAGAACAGC 
GAGGAGTCCG ACCAAGGGAA ATACGAGTGT GTTGCCATGA ACAGCGCTGG GACCCGTTAC 
TCCGCTCCTG CCAATCTCTA CGTACGAGTG CGAAGAGTCC CACCCAGATT CTCCATTCCG 
CCCACCAACC AAGAGGTGAT GCCAGGCGGC AGCGTCAACC TGACATGCGT GGCAGTGGGC 
TCGCCGATGC CCTACGTCAA GTGGACGATG GGCCTGGTGG AGCTGACCAA GGAGGAGGAG 
ATGCCGATGG GACGCAACGT GCTGGAGGTC AACAACATCC GCGAGTCGGC GAACTACACC 
TGCGTGGCCA TCTCCTCTCT GGGCATGATA GAAGCCACCG CTCAGGTCAC CGTCAAAGCT 
TTGCCAAAGC CTCCCGCCTC CCTCATTGTG ACTGAGACCA CAGCCACCAG TGTGACTCTC 
ACCTGGGACT CTGGAAACCC AGAACCTGTC TCCTACTACA TGATCCAATA CAGAGCCAAG 
ACCTCGGACA ACGGCTTCCA GGAAGTGGAA GGTGTGGCAA CTACCCGTTA CAGCATCGGT 
GGACTTAGCC CCTACTCTGA GTATGAGTTT CGTGTCATGG CTGTCAACAA CATCGGCCGG 
GGGCCACCGA GCGACCCCGT GGAGACACGC ACTGGTGAAC AGGCCCCCTC CTCGCCACCT 
CTGCACGTCC AAGCGCGCAT GCTGAGTGCC AGCACAATGC TGGTCCAGTG GGAACCGCCC 
GAGGAACCCA ACGGGCAAAT CAGGGGCTAC CGGGTCTACT ACAGCTCGGA CTTGACGGCT 
CCGCTCAGCG CCTGGCAGAA GCACAACACA GACGACAGCA GCCTGACGAC CATCTCGGGC 
CTGACTCCTG ATATCACCTA CAGCCTCAGG GTGCTGGGCT TCACCTCTGT AGGTGACGGG 
CCACCTTCTG ACATCCTGCA AGTCAAAACC CAACAGGGAG TCCCTGCCCA GCCGTCCAGC 
TTTGAGGCCG AAGCTGAGCT GGACAGTCGC ATCATGCTAA CATGGCTGTG GCCCGTCCAG 
GACCCCATCA CTAAGTATGA GCTGCAGTAC TGGGAGGCCG GCTCAGACAA CAAGTTACAT 
GTGAGCTTCA ACCCAGCGGG CTCCTACGCT GTGGACGGTC TAAAGCCTGA CACCTTGTAC 
CGTTTCTCTC TGGCAGCCCG TTCTGAGATG GGCTTAGGTG TCTACACCCA ACCCATCGAG 
GCTCGCACCG CCCAGTCCAC CCCATCAGCT CCCCCCCAGG AGGTACACCT GGTCAGCCTG 
AGCTCCACCA GCCTCAAGGT GAGTTGGGTG GCACCGCCCG CCGCTAGCCG CCACGGCGCC 
ATTGTGCGCT ACACAGTCAG CTACCAGGCC ATGGCTGGAG AAGACACGGA GCGGCACGAG 
GTGACGGGCA TCGGCGCAGA CGCCACTAGC TTTGTGCTGG AGGGCCTGGA GAAGTGGACT 
GAGTATCAGG TGTGGGTGAG GGCACACACT GACGTGGGCC CCGGCCCAGA GAGTGCCCCA 
GTGAGGATGA GAACCAAAGA GGATGTGCCG GGAGCACCCC CCAGGAAGCT GGAGGTTGAG 
GCCATAAACT CCACAGCAAT CCGGGTCACC TGGAAGCCGC CCCTGCAGGG GAAGCAGCAC 
GGCCAAATCC GGGGCTATCA AGTCATCTTC TCTCGGCTTG AAAACGGCGA ACCACGAGGC 
CAGCCTAACA TCATGGACGT TGCTTTGCCA GAGGCACAGT GGAATATTGA GGAGTCCACC 
GAGCATGAGG CCATTATTGC CGGACTGCAC TCTGAAACCA CCTACTCGGT CACCGTGGCA 
GCATACACCA CCAAAGGAGA CGGAGCGCGC AGCAAAGCCA AAGTCATCAC CACGACGGGT 
GCAGTGCCAG GAAAGCCCAC CATGATGATC AGCACCACTA TGGGCAACAC AGCGCTGATT 
CAGTGGCAGC CGCCTAAGGA AATGGTTGGG GAGCTGATGG GCTACCAGCT GCAGTACAAG 
CGCACTGACG AGGAAAACTA CACCTCCCGC GAGCTGAGAA AGATCGATGA CCATTACACC 
GTCACCGGGC TGCACAAAGG TGCCACCTAT GTCTTCCGCC TCAGTGCCCG CAACCGCGCG 
GGCATCGGCG AAGCATACGT GAAAGAGATC AGCACCCCTG AAGACGTGCC GTCGGGCTTC 
CCGTTCAACC TGCAGGTCAC GGGACTGACT CAGTCCAGCA CCGAGCTGAC CTGGGACCCT 
CCGCTGGTGG CCGAAAGGAA CGGCAAGATC GTGCACTATG TGGTCGTTTA CAGGGACATC 
AACAGTCAGC AGAACAGCAC CAACCGGACG AGCGAAACGC ACATGACCAT CCAGGGCCTT 
AATCCCGACA CCACCTACGA TATCCGGGTG CAGGCGTTCA CCAGTAAGGG CGGAGGACCC 
CTCAGCCCTA GCATCCAGAG CAGGACCATG TCGAACGATT TTCCCACCCT CAACTTTGGT 
GTCAAAGCCG TCATGAAAAC ATCTGTCCTC CTCACCTGGG ATTTGCCACC GAACTACAAA 
TCCCAAGTGC CTTTCAAGAT CCTGTACAAC CAGCAAAGCG TGGAAGTGCA GGGATCCCTG 
AAGAGGAGGC TGATCACGCA GCTGCAGCCA GACACGGACT ACTCTTTTGT GCTGACAAGC 
AGGGGCAACA TTGCTGGTGG TCTACAGCAG CAGGTGTCCA TCCGCACCGC TCCCGACCTG 
ATCAACGCCA AGCCCAGCAC ACATCAGCCT CAGGGTAGCA AGATGACAAT CAACCTGCCC 
AAGGTCCAGA CCACCACGCG TGTCAGGTGG TACTACATCG TGGTGGTGCC GGTGTCCCAG 
TCAACGCAGG GGTGGAAGAA TCCAGACGAG ATGGACCTGG ACGAGCTGTT CAAGTCAAGT 
GAAGGCCCCG TCCTGAGGCG CAGACGTCAC CTGGATTCCT CCAAACCTTA TATCGCTGCC 
AAGTTGGCCT CGTTGCCGAC CACCTTCACC CTGGGTGATG AGAAGAGATA CAACGGCTTC 
TACAACAAGC CCCTGAGCAG TCCGCAGGAG TATCTCTGCT TTGTCCTGGC AGTACTCCGA 
TCCGAAGGGG CAGACAGCAC TACTAATTAT ATGACATTTT CAGCCAGTCC CTACTCAGAC 
CCGGTCCACG TGCGAAACAG CATGGCTCAG GGGATGGAGC AGCCTGAAAT GTTGTGGGTT 
ATGGGCCCTG TTTTGGCCGT GGTCCTGATT ATCATCATCG TCATCGCCAT CCTGCTCTTC 
AAGAAGAAGC GAGCGTCGCC CCTACCCAAA GATGAGCACT CGGGCGGGGT GAAGGACTCC 
CTGCTGGCCA ACTCCTCCGA CCCTGTGGAG ATGAGAAGAC TTAACTACCA GACCCCAGGA 
ATGAGGGAGC ACCCGCCCAT CCCTGTCGTC GACCTGGCTG ACCACATAGA GCGTCTGAAG 
GCCAACGACG GCCTACGCTT TTCCCAGGAG TACGAGTCGA TTGATCCAGG GCAGCAGTTT 
ACCTGGGAGA ACTCCAACAT GGAAGTCAAC AAGCCAAAGA ATCGCTATGC AAACGTCATC 
GCCTATGACC ACTCCAGAGT GGTTCTCACC TCTGTTGACG CCGTTCCAGG CAGTGACTAC 
ATCAACGCCA ACTACATCGA CGGCTACAGG AAGCAGAACG CCTACATCGC CACGCAGGGG 
CCACTTCCAG AGACACTGAG CGACTTCTGG AGGATGGTGT GGGAGCAAAG ATCCAACACC 
ATCGTCATGA TGACCAGACT GGAGGAGAAG TCGCGGGTGA AGTGTGACCA GTACTGGCCG 
TCGCGAGGAA CGGAGACCTA TGGGATGATC CAGGTCACCA TGCTGGACAC CGTGGAGTTG 
GCCACTTACA GCGTCCGTAC CTTTGCCCTC TATAAGAATG GCTCCAGTGA GAAGAGAGAG 
GTGAGACAGT TCCAGTTCAT GGCCTGGCCA GACCACGGGG TGCCAGAGTA CCCCACCCCA 
ATCCTGGCCT TCCTACGGCG GGTAAAGGCC TGCAATCCTC CAGACGCAGG GCCTATGGTG 
GTCCACTGCA GCGCGGGTGT GGGCAGGACC GGATGCTTCA TCGTGATAGA TGCCATGCTG 
GAGCGCATGA AGCACGAGAA GTCCGTAGAC ATCTACGGCC ACGTGACGTG CATGCGCGCC 
CAGAGGAACT ACATGGTCCA GACCGAGGAC CAGTACATCT TCATTCACGA AGCCCTGCTG 
GAGGCCGCCA CGTGCGGCAA CACCGAGGTG CCGGCGCGCA ACCTGTACGC CCACATCCAG 
AAACTCACCC AGCCCCCGCC CGGCGAGACG GTTACTGCCA TGGAGCTGGA ATTCAAGAGA 
CTGGCCAACT CCAAAGCCCA CACATCCCGC TTCATCAGCG CCAACTTGCC CTGCAACAAG 
TTCAAGAACC GCCTGGTCAA CATCATGCCT TTCGAGTCCA CCCGCGTCTG CCTGCAGCCC 
ATCCGTGGCG TCGAGGGCTC CGACTACATC AATGCCAGCT GCATCGACGG CTACAGACAG 
CAGAAGGCCT ACCTGGCCAC GCAGGGCCCA CTGGCAGAGA CCACCGAGGA CTTCTGGAGG 
ATGCTGTGGG AGCACAACTC CACCATCGTC GTCATGCTCA CCAAGCTACG AGAGATGGGC 
CGGGAGAAGT GCCATCAGTA CTGGCCAGCA GAGCGTTCAG CCAGGTACCA GTACTTTGTG 
GTGGATCCAA TGGCTGAGTA CAACATGCCC CAGTACATCC TCAGAGAGTT CAAAGTCACA 
GATGCCAGGG ACGGCCAGTC CAGAACAATC CGCCAATTCC AGTTCACTGA CTGGCCCGAG 
CAAGGAGTGC CAAAAACCGG AGAGGGCTTC ATTGATTTTA TCGGACAAGT GCACAAAACC 
AAGGAACAGT TCGGACAGGA CGGACCAATC ACTGTACACT GCAGTGCTGG GGTGGGCAGG 
ACCGGAGTCT TCATCACCCT CAGCATCGTG CTGGAGAGGA TGAGGTATGA AGGAGTTGTC 
GACCTCTTCC AGACAGTGAA GACACTTCGC ACCCAGCGGC CTGCCATGGT GCAGACCGAG 
GACCAGTACC AGTTGTGCTA CAGGGCAGCA TTGGAGTACC TGGGGAGCTT TGACCACTAT 
GCAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010744752.2
CDS	483..6191
Translation	MVPNIHLAVPFLPRGALLFLILLSCVVFSSLADSMPSFVKSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVEKTRTATMLCAASGNPDPEISWFKDMLPVDISSSNGRIKQLRSGALQIENSEESDQGKYECVAMNSAGTRYSAPANLYVRVRRVPPRFSIPPTNQEVMPGGSVNLTCVAVGSPMPYVKWTMGLVELTKEEEMPMGRNVLEVNNIRESANYTCVAISSLGMIEATAQVTVKALPKPPASLIVTETTATSVTLTWDSGNPEPVSYYMIQYRAKTSDNGFQEVEGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSDPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRVYYSSDLTAPLSAWQKHNTDDSSLTTISGLTPDITYSLRVLGFTSVGDGPPSDILQVKTQQGVPAQPSSFEAEAELDSRIMLTWLWPVQDPITKYELQYWEAGSDNKLHVSFNPAGSYAVDGLKPDTLYRFSLAARSEMGLGVYTQPIEARTAQSTPSAPPQEVHLVSLSSTSLKVSWVAPPAASRHGAIVRYTVSYQAMAGEDTERHEVTGIGADATSFVLEGLEKWTEYQVWVRAHTDVGPGPESAPVRMRTKEDVPGAPPRKLEVEAINSTAIRVTWKPPLQGKQHGQIRGYQVIFSRLENGEPRGQPNIMDVALPEAQWNIEESTEHEAIIAGLHSETTYSVTVAAYTTKGDGARSKAKVITTTGAVPGKPTMMISTTMGNTALIQWQPPKEMVGELMGYQLQYKRTDEENYTSRELRKIDDHYTVTGLHKGATYVFRLSARNRAGIGEAYVKEISTPEDVPSGFPFNLQVTGLTQSSTELTWDPPLVAERNGKIVHYVVVYRDINSQQNSTNRTSETHMTIQGLNPDTTYDIRVQAFTSKGGGPLSPSIQSRTMSNDFPTLNFGVKAVMKTSVLLTWDLPPNYKSQVPFKILYNQQSVEVQGSLKRRLITQLQPDTDYSFVLTSRGNIAGGLQQQVSIRTAPDLINAKPSTHQPQGSKMTINLPKVQTTTRVRWYYIVVVPVSQSTQGWKNPDEMDLDELFKSSEGPVLRRRRHLDSSKPYIAAKLASLPTTFTLGDEKRYNGFYNKPLSSPQEYLCFVLAVLRSEGADSTTNYMTFSASPYSDPVHVRNSMAQGMEQPEMLWVMGPVLAVVLIIIIVIAILLFKKKRASPLPKDEHSGGVKDSLLANSSDPVEMRRLNYQTPGMREHPPIPVVDLADHIERLKANDGLRFSQEYESIDPGQQFTWENSNMEVNKPKNRYANVIAYDHSRVVLTSVDAVPGSDYINANYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRSNTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVTMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRAQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPPPGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYLATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_010746450.3
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010746450.3

ORF Insert Sequence:

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTGGACATC 
AGCAGCAGCA ACGGGCGCAT TAAACAGCTT CGTTCAGGAG CTCTGCAAAT TGAGAACAGC 
GAGGAGTCCG ACCAAGGGAA ATACGAGTGT GTTGCCATGA ACAGCGCTGG GACCCGTTAC 
TCCGCTCCTG CCAATCTCTA CGTACGAGTG CGAAGAGTCC CACCCAGATT CTCCATTCCG 
CCCACCAACC AAGAGGTGAT GCCAGGCGGC AGCGTCAACC TGACATGCGT GGCAGTGGGC 
TCGCCGATGC CCTACGTCAA GTGGACGATG GGCCTGGTGG AGCTGACCAA GGAGGAGGAG 
ATGCCGATGG GACGCAACGT GCTGGAGGTC AACAACATCC GCGAGTCGGC GAACTACACC 
TGCGTGGCCA TCTCCTCTCT GGGCATGATA GAAGCCACCG CTCAGGTCAC CGTCAAAGCT 
TTGCCAAAGC CTCCCGCCTC CCTCATTGTG ACTGAGACCA CAGCCACCAG TGTGACTCTC 
ACCTGGGACT CTGGAAACCC AGAACCTGTC TCCTACTACA TGATCCAATA CAGAGCCAAG 
ACCTCGGACA ACGGCTTCCA GGAAGTGGAA GGTGTGGCAA CTACCCGTTA CAGCATCGGT 
GGACTTAGCC CCTACTCTGA GTATGAGTTT CGTGTCATGG CTGTCAACAA CATCGGCCGG 
GGGCCACCGA GCGACCCCGT GGAGACACGC ACTGGTGAAC AGGCCCCCTC CTCGCCACCT 
CTGCACGTCC AAGCGCGCAT GCTGAGTGCC AGCACAATGC TGGTCCAGTG GGAACCGCCC 
GAGGAACCCA ACGGGCAAAT CAGGGGCTAC CGGGTCTACT ACAGCTCGGA CTTGACGGCT 
CCGCTCAGCG CCTGGCAGAA GCACAACACA GACGACAGCA GCCTGACGAC CATCTCGGGC 
CTGACTCCTG ATATCACCTA CAGCCTCAGG GTGCTGGGCT TCACCTCTGT AGGTGACGGG 
CCACCTTCTG ACATCCTGCA AGTCAAAACC CAACAGGGAG TCCCTGCCCA GCCGTCCAGC 
TTTGAGGCCG AAGCTGAGCT GGACAGTCGC ATCATGCTAA CATGGCTGTG GCCCGTCCAG 
GACCCCATCA CTAAGTATGA GCTGCAGTAC TGGGAGGCCG GCTCAGACAA CAAGTTACAT 
GTGAGCTTCA ACCCAGCGGG CTCCTACGCT GTGGACGGTC TAAAGCCTGA CACCTTGTAC 
CGTTTCTCTC TGGCAGCCCG TTCTGAGATG GGCTTAGGTG TCTACACCCA ACCCATCGAG 
GCTCGCACCG CCCAGTCCAC CCCATCAGCT CCCCCCCAGG AGGTACACCT GGTCAGCCTG 
AGCTCCACCA GCCTCAAGGT GAGTTGGGTG GCACCGCCCG CCGCTAGCCG CCACGGCGCC 
ATTGTGCGCT ACACAGTCAG CTACCAGGCC ATGGCTGGAG AAGACACGGA GCGGCACGAG 
GTGACGGGCA TCGGCGCAGA CGCCACTAGC TTTGTGCTGG AGGGCCTGGA GAAGTGGACT 
GAGTATCAGG TGTGGGTGAG GGCACACACT GACGTGGGCC CCGGCCCAGA GAGTGCCCCA 
GTGAGGATGA GAACCAAAGA GGATGTGCCG GGAGCACCCC CCAGGAAGCT GGAGGTTGAG 
GCCATAAACT CCACAGCAAT CCGGGTCACC TGGAAGCCGC CCCTGCAGGG GAAGCAGCAC 
GGCCAAATCC GGGGCTATCA AGTCATCTTC TCTCGGCTTG AAAACGGCGA ACCACGAGGC 
CAGCCTAACA TCATGGACGT TGCTTTGCCA GAGGCACAGT GGAATATTGA GGAGTCCACC 
GAGCATGAGG CCATTATTGC CGGACTGCAC TCTGAAACCA CCTACTCGGT CACCGTGGCA 
GCATACACCA CCAAAGGAGA CGGAGCGCGC AGCAAAGCCA AAGTCATCAC CACGACGGGT 
GCAGTGCCAG GAAAGCCCAC CATGATGATC AGCACCACTA TGGGCAACAC AGCGCTGATT 
CAGTGGCAGC CGCCTAAGGA AATGGTTGGG GAGCTGATGG GCTACCAGCT GCAGTACAAG 
CGCACTGACG AGGAAAACTA CACCTCCCGC GAGCTGAGAA AGATCGATGA CCATTACACC 
GTCACCGGGC TGCACAAAGG TGCCACCTAT GTCTTCCGCC TCAGTGCCCG CAACCGCGCG 
GGCATCGGCG AAGCATACGT GAAAGAGATC AGCACCCCTG AAGACGTGCC GTCGGGCTTC 
CCGTTCAACC TGCAGGTCAC GGGACTGACT CAGTCCAGCA CCGAGCTGAC CTGGGACCCT 
CCGCTGGTGG CCGAAAGGAA CGGCAAGATC GTGCACTATG TGGTCGTTTA CAGGGACATC 
AACAGTCAGC AGAACAGCAC CAACCGGACG AGCGAAACGC ACATGACCAT CCAGGGCCTT 
AATCCCGACA CCACCTACGA TATCCGGGTG CAGGCGTTCA CCAGTAAGGG CGGAGGACCC 
CTCAGCCCTA GCATCCAGAG CAGGACCATG TCGAACGATT TTCCCACCCT CAACTTTGGT 
GTCAAAGCCG TCATGAAAAC ATCTGTCCTC CTCACCTGGG ATTTGCCACC GAACTACAAA 
TCCCAAGTGC CTTTCAAGAT CCTGTACAAC CAGCAAAGCG TGGAAGTGCA GGGATCCCTG 
AAGAGGAGGC TGATCACGCA GCTGCAGCCA GACACGGACT ACTCTTTTGT GCTGACAAGC 
AGGGGCAACA TTGCTGGTGG TCTACAGCAG CAGGTGTCCA TCCGCACCGC TCCCGACCTG 
ATCAACGCCA AGCCCAGCAC ACATCAGCCT CAGGGTAGCA AGATGACAAT CAACCTGCCC 
AAGGTCCAGA CCACCACGCG TGTCAGGTGG TACTACATCG TGGTGGTGCC GGTGTCCCAG 
TCAACGCAGG GGTGGAAGAA TCCAGACGAG ATGGACCTGG ACGAGCTGTT CAAGTCAAGT 
GAAGGCCCCG TCCTGAGGCG CAGACGTCAC CTGGATTCCT CCAAACCTTA TATCGCTGCC 
AAGTTGGCCT CGTTGCCGAC CACCTTCACC CTGGGTGATG AGAAGAGATA CAACGGCTTC 
TACAACAAGC CCCTGAGCAG TCCGCAGGAG TATCTCTGCT TTGTCCTGGC AGTACTCCGA 
TCCGAAGGGG CAGACAGCAC TACTAATTAT ATGACATTTT CAGCCAGTCC CTACTCAGAC 
CCGGTCCACG TGCGAAACAG CATGGCTCAG GGGATGGAGC AGCCTGAAAT GTTGTGGGTT 
ATGGGCCCTG TTTTGGCCGT GGTCCTGATT ATCATCATCG TCATCGCCAT CCTGCTCTTC 
AAGAAGAAGC GAGCGTCGCC CCTACCCAAA GATGAGCACT CGGGCGGGGT GAAGGACTCC 
CTGCTGGCCA ACTCCTCCGA CCCTGTGGAG ATGAGAAGAC TTAACTACCA GACCCCAGGA 
ATGAGGGAGC ACCCGCCCAT CCCTGTCGTC GACCTGGCTG ACCACATAGA GCGTCTGAAG 
GCCAACGACG GCCTACGCTT TTCCCAGGAG TACGAGTCGA TTGATCCAGG GCAGCAGTTT 
ACCTGGGAGA ACTCCAACAT GGAAGTCAAC AAGCCAAAGA ATCGCTATGC AAACGTCATC 
GCCTATGACC ACTCCAGAGT GGTTCTCACC TCTGTTGACG CCGTTCCAGG CAGTGACTAC 
ATCAACGCCA ACTACATCGA CGGCTACAGG AAGCAGAACG CCTACATCGC CACGCAGGGG 
CCACTTCCAG AGACACTGAG CGACTTCTGG AGGATGGTGT GGGAGCAAAG ATCCAACACC 
ATCGTCATGA TGACCAGACT GGAGGAGAAG TCGCGGGTGA AGTGTGACCA GTACTGGCCG 
TCGCGAGGAA CGGAGACCTA TGGGATGATC CAGGTCACCA TGCTGGACAC CGTGGAGTTG 
GCCACTTACA GCGTCCGTAC CTTTGCCCTC TATAAGAATG GCTCCAGTGA GAAGAGAGAG 
GTGAGACAGT TCCAGTTCAT GGCCTGGCCA GACCACGGGG TGCCAGAGTA CCCCACCCCA 
ATCCTGGCCT TCCTACGGCG GGTAAAGGCC TGCAATCCTC CAGACGCAGG GCCTATGGTG 
GTCCACTGCA GCGCGGGTGT GGGCAGGACC GGATGCTTCA TCGTGATAGA TGCCATGCTG 
GAGCGCATGA AGCACGAGAA GTCCGTAGAC ATCTACGGCC ACGTGACGTG CATGCGCGCC 
CAGAGGAACT ACATGGTCCA GACCGAGGAC CAGTACATCT TCATTCACGA AGCCCTGCTG 
GAGGCCGCCA CGTGCGGCAA CACCGAGGTG CCGGCGCGCA ACCTGTACGC CCACATCCAG 
AAACTCACCC AGCCCCCGCC CGGCGAGACG GTTACTGCCA TGGAGCTGGA ATTCAAGAGA 
CTGGCCAACT CCAAAGCCCA CACATCCCGC TTCATCAGCG CCAACTTGCC CTGCAACAAG 
TTCAAGAACC GCCTGGTCAA CATCATGCCT TTCGAGTCCA CCCGCGTCTG CCTGCAGCCC 
ATCCGTGGCG TCGAGGGCTC CGACTACATC AATGCCAGCT GCATCGACGG CTACAGACAG 
CAGAAGGCCT ACCTGGCCAC GCAGGGCCCA CTGGCAGAGA CCACCGAGGA CTTCTGGAGG 
ATGCTGTGGG AGCACAACTC CACCATCGTC GTCATGCTCA CCAAGCTACG AGAGATGGGC 
CGGGAGAAGT GCCATCAGTA CTGGCCAGCA GAGCGTTCAG CCAGGTACCA GTACTTTGTG 
GTGGATCCAA TGGCTGAGTA CAACATGCCC CAGTACATCC TCAGAGAGTT CAAAGTCACA 
GATGCCAGGG ACGGCCAGTC CAGAACAATC CGCCAATTCC AGTTCACTGA CTGGCCCGAG 
CAAGGAGTGC CAAAAACCGG AGAGGGCTTC ATTGATTTTA TCGGACAAGT GCACAAAACC 
AAGGAACAGT TCGGACAGGA CGGACCAATC ACTGTACACT GCAGTGCTGG GGTGGGCAGG 
ACCGGAGTCT TCATCACCCT CAGCATCGTG CTGGAGAGGA TGAGGTATGA AGGAGTTGTC 
GACCTCTTCC AGACAGTGAA GACACTTCGC ACCCAGCGGC CTGCCATGGT GCAGACCGAG 
GACCAGTACC AGTTGTGCTA CAGGGCAGCA TTGGAGTACC TGGGGAGCTT TGACCACTAT 
GCAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104931439 ( XM_010746453.3 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010744755.2 Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X13, mRNA.

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CloneID	OLa211357
Clone ID Related Accession (Same CDS sequence)	XM_010746453.3
Accession Version	XM_010746453.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	receptor-type tyrosine-protein phosphatase F isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040012.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_010746453.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTGGACATC 
AGCAGCAGCA ACGGGCGCAT TAAACAGCTT CGTTCAGGAG CTCTGCAAAT TGAGAACAGC 
GAGGAGTCCG ACCAAGGGAA ATACGAGTGT GTTGCCATGA ACAGCGCTGG GACCCGTTAC 
TCCGCTCCTG CCAATCTCTA CGTACGAGTG CGAAGAGTCC CACCCAGATT CTCCATTCCG 
CCCACCAACC AAGAGGTGAT GCCAGGCGGC AGCGTCAACC TGACATGCGT GGCAGTGGGC 
TCGCCGATGC CCTACGTCAA GTGGACGATG GGCCTGGTGG AGCTGACCAA GGAGGAGGAG 
ATGCCGATGG GACGCAACGT GCTGGAGGTC AACAACATCC GCGAGTCGGC GAACTACACC 
TGCGTGGCCA TCTCCTCTCT GGGCATGATA GAAGCCACCG CTCAGGTCAC CGTCAAAGCT 
TTGCCAAAGC CTCCCGCCTC CCTCATTGTG ACTGAGACCA CAGCCACCAG TGTGACTCTC 
ACCTGGGACT CTGGAAACCC AGAACCTGTC TCCTACTACA TGATCCAATA CAGAGCCAAG 
ACCTCGGACA ACGGCTTCCA GGAAGTGGAA GGTGTGGCAA CTACCCGTTA CAGCATCGGT 
GGACTTAGCC CCTACTCTGA GTATGAGTTT CGTGTCATGG CTGTCAACAA CATCGGCCGG 
GGGCCACCGA GCGACCCCGT GGAGACACGC ACTGGTGAAC AGGCCCCCTC CTCGCCACCT 
CTGCACGTCC AAGCGCGCAT GCTGAGTGCC AGCACAATGC TGGTCCAGTG GGAACCGCCC 
GAGGAACCCA ACGGGCAAAT CAGGGGCTAC CGGGTCTACT ACAGCTCGGA CTTGACGGCT 
CCGCTCAGCG CCTGGCAGAA GCACAACACA GACGACAGCA GCCTGACGAC CATCTCGGGC 
CTGACTCCTG ATATCACCTA CAGCCTCAGG GTGCTGGGCT TCACCTCTGT AGGTGACGGG 
CCACCTTCTG ACATCCTGCA AGTCAAAACC CAACAGGGAG TCCCTGCCCA GCCGTCCAGC 
TTTGAGGCCG AAGCTGAGCT GGACAGTCGC ATCATGCTAA CATGGCTGTG GCCCGTCCAG 
GACCCCATCA CTAAGTATGA GCTGCAGTAC TGGGAGGCCG GCTCAGACAA CAAGTTACAT 
GTGAGCTTCA ACCCAGCGGG CTCCTACGCT GTGGACGGTC TAAAGCCTGA CACCTTGTAC 
CGTTTCTCTC TGGCAGCCCG TTCTGAGATG GGCTTAGGTG TCTACACCCA ACCCATCGAG 
GCTCGCACCG CCCAGTCCAC CCCATCAGCT CCCCCCCAGG AGGTACACCT GGTCAGCCTG 
AGCTCCACCA GCCTCAAGGT GAGTTGGGTG GCACCGCCCG CCGCTAGCCG CCACGGCGCC 
ATTGTGCGCT ACACAGTCAG CTACCAGGCC ATGGCTGGAG AAGACACGGA GCGGCACGAG 
GTGACGGGCA TCGGCGCAGA CGCCACTAGC TTTGTGCTGG AGGGCCTGGA GAAGTGGACT 
GAGTATCAGG TGTGGGTGAG GGCACACACT GACGTGGGCC CCGGCCCAGA GAGTGCCCCA 
GTGAGGATGA GAACCAAAGA GGATGTGCCG GGAGCACCCC CCAGGAAGCT GGAGGTTGAG 
GCCATAAACT CCACAGCAAT CCGGGTCACC TGGAAGCCGC CCCTGCAGGG GAAGCAGCAC 
GGCCAAATCC GGGGCTATCA AGTCATCTTC TCTCGGCTTG AAAACGGCGA ACCACGAGGC 
CAGCCTAACA TCATGGACGT TGCTTTGCCA GAGGCACAGG AGGCCATTAT TGCCGGACTG 
CACTCTGAAA CCACCTACTC GGTCACCGTG GCAGCATACA CCACCAAAGG AGACGGAGCG 
CGCAGCAAAG CCAAAGTCAT CACCACGACG GGTGCAGTGC CAGGAAAGCC CACCATGATG 
ATCAGCACCA CTATGGGCAA CACAGCGCTG ATTCAGTGGC AGCCGCCTAA GGAAATGGTT 
GGGGAGCTGA TGGGCTACCA GCTGCAGTAC AAGCGCACTG ACGAGGAAAA CTACACCTCC 
CGCGAGCTGA GAAAGATCGA TGACCATTAC ACCGTCACCG GGCTGCACAA AGGTGCCACC 
TATGTCTTCC GCCTCAGTGC CCGCAACCGC GCGGGCATCG GCGAAGCATA CGTGAAAGAG 
ATCAGCACCC CTGAAGACGT GCCGTCGGGC TTCCCGTTCA ACCTGCAGGT CACGGGACTG 
ACTCAGTCCA GCACCGAGCT GACCTGGGAC CCTCCGCTGG TGGCCGAAAG GAACGGCAAG 
ATCGTGCACT ATGTGGTCGT TTACAGGGAC ATCAACAGTC AGCAGAACAG CACCAACCGG 
ACGAGCGAAA CGCACATGAC CATCCAGGGC CTTAATCCCG ACACCACCTA CGATATCCGG 
GTGCAGGCGT TCACCAGTAA GGGCGGAGGA CCCCTCAGCC CTAGCATCCA GAGCAGGACC 
ATGTCGAACG ATTTTCCCAC CCTCAACTTT GGTGTCAAAG CCGTCATGAA AACATCTGTC 
CTCCTCACCT GGGATTTGCC ACCGAACTAC AAATCCCAAG TGCCTTTCAA GATCCTGTAC 
AACCAGCAAA GCGTGGAAGT GCAGGGATCC CTGAAGAGGA GGCTGATCAC GCAGCTGCAG 
CCAGACACGG ACTACTCTTT TGTGCTGACA AGCAGGGGCA ACATTGCTGG TGGTCTACAG 
CAGCAGGTGT CCATCCGCAC CGCTCCCGAC CTGATCAACG CCAAGCCCAG CACACATCAG 
CCTCAGGGTA GCAAGATGAC AATCAACCTG CCCAAGGTCC AGACCACCAC GCGTGTCAGG 
TGGTACTACA TCGTGGTGGT GCCGGTGTCC CAGTCAACGC AGGGGTGGAA GAATCCAGAC 
GAGATGGACC TGGACGAGCT GTTCAAGTCA AGTGAAGGCC CCGTCCTGAG GCGCAGACGT 
CACCTGGATT CCTCCAAACC TTATATCGCT GCCAAGTTGG CCTCGTTGCC GACCACCTTC 
ACCCTGGGTG ATGAGAAGAG ATACAACGGC TTCTACAACA AGCCCCTGAG CAGTCCGCAG 
GAGTATCTCT GCTTTGTCCT GGCAGTACTC CGATCCGAAG GGGCAGACAG CACTACTAAT 
TATATGACAT TTTCAGCCAG TCCCTACTCA GACCCGGTCC ACGTGCGAAA CAGCATGGCT 
CAGGGGATGG AGCAGCCTGA AATGTTGTGG GTTATGGGCC CTGTTTTGGC CGTGGTCCTG 
ATTATCATCA TCGTCATCGC CATCCTGCTC TTCAAGAAGA AGCGAGCGTC GCCCCTACCC 
AAAGATGAGC ACTCGGGCGG GGTGAAGGAC TCCCTGCTGG CCAACTCCTC CGACCCTGTG 
GAGATGAGAA GACTTAACTA CCAGACCCCA GGAATGAGGG AGCACCCGCC CATCCCTGTC 
GTCGACCTGG CTGACCACAT AGAGCGTCTG AAGGCCAACG ACGGCCTACG CTTTTCCCAG 
GAGTACGAGT CGATTGATCC AGGGCAGCAG TTTACCTGGG AGAACTCCAA CATGGAAGTC 
AACAAGCCAA AGAATCGCTA TGCAAACGTC ATCGCCTATG ACCACTCCAG AGTGGTTCTC 
ACCTCTGTTG ACGCCGTTCC AGGCAGTGAC TACATCAACG CCAACTACAT CGACGGCTAC 
AGGAAGCAGA ACGCCTACAT CGCCACGCAG GGGCCACTTC CAGAGACACT GAGCGACTTC 
TGGAGGATGG TGTGGGAGCA AAGATCCAAC ACCATCGTCA TGATGACCAG ACTGGAGGAG 
AAGTCGCGGG TGAAGTGTGA CCAGTACTGG CCGTCGCGAG GAACGGAGAC CTATGGGATG 
ATCCAGGTCA CCATGCTGGA CACCGTGGAG TTGGCCACTT ACAGCGTCCG TACCTTTGCC 
CTCTATAAGA ATGGCTCCAG TGAGAAGAGA GAGGTGAGAC AGTTCCAGTT CATGGCCTGG 
CCAGACCACG GGGTGCCAGA GTACCCCACC CCAATCCTGG CCTTCCTACG GCGGGTAAAG 
GCCTGCAATC CTCCAGACGC AGGGCCTATG GTGGTCCACT GCAGCGCGGG TGTGGGCAGG 
ACCGGATGCT TCATCGTGAT AGATGCCATG CTGGAGCGCA TGAAGCACGA GAAGTCCGTA 
GACATCTACG GCCACGTGAC GTGCATGCGC GCCCAGAGGA ACTACATGGT CCAGACCGAG 
GACCAGTACA TCTTCATTCA CGAAGCCCTG CTGGAGGCCG CCACGTGCGG CAACACCGAG 
GTGCCGGCGC GCAACCTGTA CGCCCACATC CAGAAACTCA CCCAGCCCCC GCCCGGCGAG 
ACGGTTACTG CCATGGAGCT GGAATTCAAG AGACTGGCCA ACTCCAAAGC CCACACATCC 
CGCTTCATCA GCGCCAACTT GCCCTGCAAC AAGTTCAAGA ACCGCCTGGT CAACATCATG 
CCTTTCGAGT CCACCCGCGT CTGCCTGCAG CCCATCCGTG GCGTCGAGGG CTCCGACTAC 
ATCAATGCCA GCTGCATCGA CGGCTACAGA CAGCAGAAGG CCTACCTGGC CACGCAGGGC 
CCACTGGCAG AGACCACCGA GGACTTCTGG AGGATGCTGT GGGAGCACAA CTCCACCATC 
GTCGTCATGC TCACCAAGCT ACGAGAGATG GGCCGGGAGA AGTGCCATCA GTACTGGCCA 
GCAGAGCGTT CAGCCAGGTA CCAGTACTTT GTGGTGGATC CAATGGCTGA GTACAACATG 
CCCCAGTACA TCCTCAGAGA GTTCAAAGTC ACAGATGCCA GGGACGGCCA GTCCAGAACA 
ATCCGCCAAT TCCAGTTCAC TGACTGGCCC GAGCAAGGAG TGCCAAAAAC CGGAGAGGGC 
TTCATTGATT TTATCGGACA AGTGCACAAA ACCAAGGAAC AGTTCGGACA GGACGGACCA 
ATCACTGTAC ACTGCAGTGC TGGGGTGGGC AGGACCGGAG TCTTCATCAC CCTCAGCATC 
GTGCTGGAGA GGATGAGGTA TGAAGGAGTT GTCGACCTCT TCCAGACAGT GAAGACACTT 
CGCACCCAGC GGCCTGCCAT GGTGCAGACC GAGGACCAGT ACCAGTTGTG CTACAGGGCA 
GCATTGGAGT ACCTGGGGAG CTTTGACCAC TATGCAACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010744755.2
CDS	481..6162
Translation	MVPNIHLAVPFLPRGALLFLILLSCVVFSSLADSMPSFVKSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVEKTRTATMLCAASGNPDPEISWFKDMLPVDISSSNGRIKQLRSGALQIENSEESDQGKYECVAMNSAGTRYSAPANLYVRVRRVPPRFSIPPTNQEVMPGGSVNLTCVAVGSPMPYVKWTMGLVELTKEEEMPMGRNVLEVNNIRESANYTCVAISSLGMIEATAQVTVKALPKPPASLIVTETTATSVTLTWDSGNPEPVSYYMIQYRAKTSDNGFQEVEGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSDPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRVYYSSDLTAPLSAWQKHNTDDSSLTTISGLTPDITYSLRVLGFTSVGDGPPSDILQVKTQQGVPAQPSSFEAEAELDSRIMLTWLWPVQDPITKYELQYWEAGSDNKLHVSFNPAGSYAVDGLKPDTLYRFSLAARSEMGLGVYTQPIEARTAQSTPSAPPQEVHLVSLSSTSLKVSWVAPPAASRHGAIVRYTVSYQAMAGEDTERHEVTGIGADATSFVLEGLEKWTEYQVWVRAHTDVGPGPESAPVRMRTKEDVPGAPPRKLEVEAINSTAIRVTWKPPLQGKQHGQIRGYQVIFSRLENGEPRGQPNIMDVALPEAQEAIIAGLHSETTYSVTVAAYTTKGDGARSKAKVITTTGAVPGKPTMMISTTMGNTALIQWQPPKEMVGELMGYQLQYKRTDEENYTSRELRKIDDHYTVTGLHKGATYVFRLSARNRAGIGEAYVKEISTPEDVPSGFPFNLQVTGLTQSSTELTWDPPLVAERNGKIVHYVVVYRDINSQQNSTNRTSETHMTIQGLNPDTTYDIRVQAFTSKGGGPLSPSIQSRTMSNDFPTLNFGVKAVMKTSVLLTWDLPPNYKSQVPFKILYNQQSVEVQGSLKRRLITQLQPDTDYSFVLTSRGNIAGGLQQQVSIRTAPDLINAKPSTHQPQGSKMTINLPKVQTTTRVRWYYIVVVPVSQSTQGWKNPDEMDLDELFKSSEGPVLRRRRHLDSSKPYIAAKLASLPTTFTLGDEKRYNGFYNKPLSSPQEYLCFVLAVLRSEGADSTTNYMTFSASPYSDPVHVRNSMAQGMEQPEMLWVMGPVLAVVLIIIIVIAILLFKKKRASPLPKDEHSGGVKDSLLANSSDPVEMRRLNYQTPGMREHPPIPVVDLADHIERLKANDGLRFSQEYESIDPGQQFTWENSNMEVNKPKNRYANVIAYDHSRVVLTSVDAVPGSDYINANYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRSNTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVTMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRAQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPPPGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYLATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_010746453.3
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010746453.3

ORF Insert Sequence:

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTGGACATC 
AGCAGCAGCA ACGGGCGCAT TAAACAGCTT CGTTCAGGAG CTCTGCAAAT TGAGAACAGC 
GAGGAGTCCG ACCAAGGGAA ATACGAGTGT GTTGCCATGA ACAGCGCTGG GACCCGTTAC 
TCCGCTCCTG CCAATCTCTA CGTACGAGTG CGAAGAGTCC CACCCAGATT CTCCATTCCG 
CCCACCAACC AAGAGGTGAT GCCAGGCGGC AGCGTCAACC TGACATGCGT GGCAGTGGGC 
TCGCCGATGC CCTACGTCAA GTGGACGATG GGCCTGGTGG AGCTGACCAA GGAGGAGGAG 
ATGCCGATGG GACGCAACGT GCTGGAGGTC AACAACATCC GCGAGTCGGC GAACTACACC 
TGCGTGGCCA TCTCCTCTCT GGGCATGATA GAAGCCACCG CTCAGGTCAC CGTCAAAGCT 
TTGCCAAAGC CTCCCGCCTC CCTCATTGTG ACTGAGACCA CAGCCACCAG TGTGACTCTC 
ACCTGGGACT CTGGAAACCC AGAACCTGTC TCCTACTACA TGATCCAATA CAGAGCCAAG 
ACCTCGGACA ACGGCTTCCA GGAAGTGGAA GGTGTGGCAA CTACCCGTTA CAGCATCGGT 
GGACTTAGCC CCTACTCTGA GTATGAGTTT CGTGTCATGG CTGTCAACAA CATCGGCCGG 
GGGCCACCGA GCGACCCCGT GGAGACACGC ACTGGTGAAC AGGCCCCCTC CTCGCCACCT 
CTGCACGTCC AAGCGCGCAT GCTGAGTGCC AGCACAATGC TGGTCCAGTG GGAACCGCCC 
GAGGAACCCA ACGGGCAAAT CAGGGGCTAC CGGGTCTACT ACAGCTCGGA CTTGACGGCT 
CCGCTCAGCG CCTGGCAGAA GCACAACACA GACGACAGCA GCCTGACGAC CATCTCGGGC 
CTGACTCCTG ATATCACCTA CAGCCTCAGG GTGCTGGGCT TCACCTCTGT AGGTGACGGG 
CCACCTTCTG ACATCCTGCA AGTCAAAACC CAACAGGGAG TCCCTGCCCA GCCGTCCAGC 
TTTGAGGCCG AAGCTGAGCT GGACAGTCGC ATCATGCTAA CATGGCTGTG GCCCGTCCAG 
GACCCCATCA CTAAGTATGA GCTGCAGTAC TGGGAGGCCG GCTCAGACAA CAAGTTACAT 
GTGAGCTTCA ACCCAGCGGG CTCCTACGCT GTGGACGGTC TAAAGCCTGA CACCTTGTAC 
CGTTTCTCTC TGGCAGCCCG TTCTGAGATG GGCTTAGGTG TCTACACCCA ACCCATCGAG 
GCTCGCACCG CCCAGTCCAC CCCATCAGCT CCCCCCCAGG AGGTACACCT GGTCAGCCTG 
AGCTCCACCA GCCTCAAGGT GAGTTGGGTG GCACCGCCCG CCGCTAGCCG CCACGGCGCC 
ATTGTGCGCT ACACAGTCAG CTACCAGGCC ATGGCTGGAG AAGACACGGA GCGGCACGAG 
GTGACGGGCA TCGGCGCAGA CGCCACTAGC TTTGTGCTGG AGGGCCTGGA GAAGTGGACT 
GAGTATCAGG TGTGGGTGAG GGCACACACT GACGTGGGCC CCGGCCCAGA GAGTGCCCCA 
GTGAGGATGA GAACCAAAGA GGATGTGCCG GGAGCACCCC CCAGGAAGCT GGAGGTTGAG 
GCCATAAACT CCACAGCAAT CCGGGTCACC TGGAAGCCGC CCCTGCAGGG GAAGCAGCAC 
GGCCAAATCC GGGGCTATCA AGTCATCTTC TCTCGGCTTG AAAACGGCGA ACCACGAGGC 
CAGCCTAACA TCATGGACGT TGCTTTGCCA GAGGCACAGG AGGCCATTAT TGCCGGACTG 
CACTCTGAAA CCACCTACTC GGTCACCGTG GCAGCATACA CCACCAAAGG AGACGGAGCG 
CGCAGCAAAG CCAAAGTCAT CACCACGACG GGTGCAGTGC CAGGAAAGCC CACCATGATG 
ATCAGCACCA CTATGGGCAA CACAGCGCTG ATTCAGTGGC AGCCGCCTAA GGAAATGGTT 
GGGGAGCTGA TGGGCTACCA GCTGCAGTAC AAGCGCACTG ACGAGGAAAA CTACACCTCC 
CGCGAGCTGA GAAAGATCGA TGACCATTAC ACCGTCACCG GGCTGCACAA AGGTGCCACC 
TATGTCTTCC GCCTCAGTGC CCGCAACCGC GCGGGCATCG GCGAAGCATA CGTGAAAGAG 
ATCAGCACCC CTGAAGACGT GCCGTCGGGC TTCCCGTTCA ACCTGCAGGT CACGGGACTG 
ACTCAGTCCA GCACCGAGCT GACCTGGGAC CCTCCGCTGG TGGCCGAAAG GAACGGCAAG 
ATCGTGCACT ATGTGGTCGT TTACAGGGAC ATCAACAGTC AGCAGAACAG CACCAACCGG 
ACGAGCGAAA CGCACATGAC CATCCAGGGC CTTAATCCCG ACACCACCTA CGATATCCGG 
GTGCAGGCGT TCACCAGTAA GGGCGGAGGA CCCCTCAGCC CTAGCATCCA GAGCAGGACC 
ATGTCGAACG ATTTTCCCAC CCTCAACTTT GGTGTCAAAG CCGTCATGAA AACATCTGTC 
CTCCTCACCT GGGATTTGCC ACCGAACTAC AAATCCCAAG TGCCTTTCAA GATCCTGTAC 
AACCAGCAAA GCGTGGAAGT GCAGGGATCC CTGAAGAGGA GGCTGATCAC GCAGCTGCAG 
CCAGACACGG ACTACTCTTT TGTGCTGACA AGCAGGGGCA ACATTGCTGG TGGTCTACAG 
CAGCAGGTGT CCATCCGCAC CGCTCCCGAC CTGATCAACG CCAAGCCCAG CACACATCAG 
CCTCAGGGTA GCAAGATGAC AATCAACCTG CCCAAGGTCC AGACCACCAC GCGTGTCAGG 
TGGTACTACA TCGTGGTGGT GCCGGTGTCC CAGTCAACGC AGGGGTGGAA GAATCCAGAC 
GAGATGGACC TGGACGAGCT GTTCAAGTCA AGTGAAGGCC CCGTCCTGAG GCGCAGACGT 
CACCTGGATT CCTCCAAACC TTATATCGCT GCCAAGTTGG CCTCGTTGCC GACCACCTTC 
ACCCTGGGTG ATGAGAAGAG ATACAACGGC TTCTACAACA AGCCCCTGAG CAGTCCGCAG 
GAGTATCTCT GCTTTGTCCT GGCAGTACTC CGATCCGAAG GGGCAGACAG CACTACTAAT 
TATATGACAT TTTCAGCCAG TCCCTACTCA GACCCGGTCC ACGTGCGAAA CAGCATGGCT 
CAGGGGATGG AGCAGCCTGA AATGTTGTGG GTTATGGGCC CTGTTTTGGC CGTGGTCCTG 
ATTATCATCA TCGTCATCGC CATCCTGCTC TTCAAGAAGA AGCGAGCGTC GCCCCTACCC 
AAAGATGAGC ACTCGGGCGG GGTGAAGGAC TCCCTGCTGG CCAACTCCTC CGACCCTGTG 
GAGATGAGAA GACTTAACTA CCAGACCCCA GGAATGAGGG AGCACCCGCC CATCCCTGTC 
GTCGACCTGG CTGACCACAT AGAGCGTCTG AAGGCCAACG ACGGCCTACG CTTTTCCCAG 
GAGTACGAGT CGATTGATCC AGGGCAGCAG TTTACCTGGG AGAACTCCAA CATGGAAGTC 
AACAAGCCAA AGAATCGCTA TGCAAACGTC ATCGCCTATG ACCACTCCAG AGTGGTTCTC 
ACCTCTGTTG ACGCCGTTCC AGGCAGTGAC TACATCAACG CCAACTACAT CGACGGCTAC 
AGGAAGCAGA ACGCCTACAT CGCCACGCAG GGGCCACTTC CAGAGACACT GAGCGACTTC 
TGGAGGATGG TGTGGGAGCA AAGATCCAAC ACCATCGTCA TGATGACCAG ACTGGAGGAG 
AAGTCGCGGG TGAAGTGTGA CCAGTACTGG CCGTCGCGAG GAACGGAGAC CTATGGGATG 
ATCCAGGTCA CCATGCTGGA CACCGTGGAG TTGGCCACTT ACAGCGTCCG TACCTTTGCC 
CTCTATAAGA ATGGCTCCAG TGAGAAGAGA GAGGTGAGAC AGTTCCAGTT CATGGCCTGG 
CCAGACCACG GGGTGCCAGA GTACCCCACC CCAATCCTGG CCTTCCTACG GCGGGTAAAG 
GCCTGCAATC CTCCAGACGC AGGGCCTATG GTGGTCCACT GCAGCGCGGG TGTGGGCAGG 
ACCGGATGCT TCATCGTGAT AGATGCCATG CTGGAGCGCA TGAAGCACGA GAAGTCCGTA 
GACATCTACG GCCACGTGAC GTGCATGCGC GCCCAGAGGA ACTACATGGT CCAGACCGAG 
GACCAGTACA TCTTCATTCA CGAAGCCCTG CTGGAGGCCG CCACGTGCGG CAACACCGAG 
GTGCCGGCGC GCAACCTGTA CGCCCACATC CAGAAACTCA CCCAGCCCCC GCCCGGCGAG 
ACGGTTACTG CCATGGAGCT GGAATTCAAG AGACTGGCCA ACTCCAAAGC CCACACATCC 
CGCTTCATCA GCGCCAACTT GCCCTGCAAC AAGTTCAAGA ACCGCCTGGT CAACATCATG 
CCTTTCGAGT CCACCCGCGT CTGCCTGCAG CCCATCCGTG GCGTCGAGGG CTCCGACTAC 
ATCAATGCCA GCTGCATCGA CGGCTACAGA CAGCAGAAGG CCTACCTGGC CACGCAGGGC 
CCACTGGCAG AGACCACCGA GGACTTCTGG AGGATGCTGT GGGAGCACAA CTCCACCATC 
GTCGTCATGC TCACCAAGCT ACGAGAGATG GGCCGGGAGA AGTGCCATCA GTACTGGCCA 
GCAGAGCGTT CAGCCAGGTA CCAGTACTTT GTGGTGGATC CAATGGCTGA GTACAACATG 
CCCCAGTACA TCCTCAGAGA GTTCAAAGTC ACAGATGCCA GGGACGGCCA GTCCAGAACA 
ATCCGCCAAT TCCAGTTCAC TGACTGGCCC GAGCAAGGAG TGCCAAAAAC CGGAGAGGGC 
TTCATTGATT TTATCGGACA AGTGCACAAA ACCAAGGAAC AGTTCGGACA GGACGGACCA 
ATCACTGTAC ACTGCAGTGC TGGGGTGGGC AGGACCGGAG TCTTCATCAC CCTCAGCATC 
GTGCTGGAGA GGATGAGGTA TGAAGGAGTT GTCGACCTCT TCCAGACAGT GAAGACACTT 
CGCACCCAGC GGCCTGCCAT GGTGCAGACC GAGGACCAGT ACCAGTTGTG CTACAGGGCA 
GCATTGGAGT ACCTGGGGAG CTTTGACCAC TATGCAACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104931439 ( XM_010746454.3 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010744756.2 Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X14, mRNA.

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CloneID	OLa211358
Clone ID Related Accession (Same CDS sequence)	XM_010746454.3
Accession Version	XM_010746454.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	receptor-type tyrosine-protein phosphatase F isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040012.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_010746454.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTGGACATC 
AGCAGCAGCA ACGGGCGCAT TAAACAGCTT CGTTCAGGAG CTCTGCAAAT TGAGAACAGC 
GAGGAGTCCG ACCAAGGGAA ATACGAGTGT GTTGCCATGA ACAGCGCTGG GACCCGTTAC 
TCCGCTCCTG CCAATCTCTA CGTACGAGTG CGAAGAGTCC CACCCAGATT CTCCATTCCG 
CCCACCAACC AAGAGGTGAT GCCAGGCGGC AGCGTCAACC TGACATGCGT GGCAGTGGGC 
TCGCCGATGC CCTACGTCAA GTGGACGATG GGCCTGGTGG AGCTGACCAA GGAGGAGGAG 
ATGCCGATGG GACGCAACGT GCTGGAGGTC AACAACATCC GCGAGTCGGC GAACTACACC 
TGCGTGGCCA TCTCCTCTCT GGGCATGATA GAAGCCACCG CTCAGGTCAC CGTCAAAGCT 
TTGCCAAAGC CTCCCGCCTC CCTCATTGTG ACTGAGACCA CAGCCACCAG TGTGACTCTC 
ACCTGGGACT CTGGAAACCC AGAACCTGTC TCCTACTACA TGATCCAATA CAGAGCCAAG 
ACCTCGGACA ACGGCTTCCA GGAAGTGGAA GGTGTGGCAA CTACCCGTTA CAGCATCGGT 
GGACTTAGCC CCTACTCTGA GTATGAGTTT CGTGTCATGG CTGTCAACAA CATCGGCCGG 
GGGCCACCGA GCGACCCCGT GGAGACACGC ACTGGTGAAC AGGCCCCCTC CTCGCCACCT 
CTGCACGTCC AAGCGCGCAT GCTGAGTGCC AGCACAATGC TGGTCCAGTG GGAACCGCCC 
GAGGAACCCA ACGGGCAAAT CAGGGGCTAC CGGGTCTACT ACAGCTCGGA CTTGACGGCT 
CCGCTCAGCG CCTGGCAGAA GCACAACACA GACGACAGCA GCCTGACGAC CATCTCGGGC 
CTGACTCCTG ATATCACCTA CAGCCTCAGG GTGCTGGGCT TCACCTCTGT AGGTGACGGG 
CCACCTTCTG ACATCCTGCA AGTCAAAACC CAACAGGGAG TCCCTGCCCA GCCGTCCAGC 
TTTGAGGCCG AAGCTGAGCT GGACAGTCGC ATCATGCTAA CATGGCTGTG GCCCGTCCAG 
GACCCCATCA CTAAGTATGA GCTGCAGTAC TGGGAGGCCG GCTCAGACAA CAAGTTACAT 
GTGAGCTTCA ACCCAGCGGG CTCCTACGCT GTGGACGGTC TAAAGCCTGA CACCTTGTAC 
CGTTTCTCTC TGGCAGCCCG TTCTGAGATG GGCTTAGGTG TCTACACCCA ACCCATCGAG 
GCTCGCACCG CCCAGTCCAT GCCGGGAGCA CCCCCCAGGA AGCTGGAGGT TGAGGCCATA 
AACTCCACAG CAATCCGGGT CACCTGGAAG CCGCCCCTGC AGGGGAAGCA GCACGGCCAA 
ATCCGGGGCT ATCAAGTCAT CTTCTCTCGG CTTGAAAACG GCGAACCACG AGGCCAGCCT 
AACATCATGG ACGTTGCTTT GCCAGAGGCA CAGGAGGCCA TTATTGCCGG ACTGCACTCT 
GAAACCACCT ACTCGGTCAC CGTGGCAGCA TACACCACCA AAGGAGACGG AGCGCGCAGC 
AAAGCCAAAG TCATCACCAC GACGGGTGCA GTGCCAGGAA AGCCCACCAT GATGATCAGC 
ACCACTATGG GCAACACAGC GCTGATTCAG TGGCAGCCGC CTAAGGAAAT GGTTGGGGAG 
CTGATGGGCT ACCAGCTGCA GTACAAGCGC ACTGACGAGG AAAACTACAC CTCCCGCGAG 
CTGAGAAAGA TCGATGACCA TTACACCGTC ACCGGGCTGC ACAAAGGTGC CACCTATGTC 
TTCCGCCTCA GTGCCCGCAA CCGCGCGGGC ATCGGCGAAG CATACGTGAA AGAGATCAGC 
ACCCCTGAAG ACGTGCCGTC GGGCTTCCCG TTCAACCTGC AGGTCACGGG ACTGACTCAG 
TCCAGCACCG AGCTGACCTG GGACCCTCCG CTGGTGGCCG AAAGGAACGG CAAGATCGTG 
CACTATGTGG TCGTTTACAG GGACATCAAC AGTCAGCAGA ACAGCACCAA CCGGACGAGC 
GAAACGCACA TGACCATCCA GGGCCTTAAT CCCGACACCA CCTACGATAT CCGGGTGCAG 
GCGTTCACCA GTAAGGGCGG AGGACCCCTC AGCCCTAGCA TCCAGAGCAG GACCATGTCG 
AACGATTTTC CCACCCTCAA CTTTGGTGTC AAAGCCGTCA TGAAAACATC TGTCCTCCTC 
ACCTGGGATT TGCCACCGAA CTACAAATCC CAAGTGCCTT TCAAGATCCT GTACAACCAG 
CAAAGCGTGG AAGTGCAGGG ATCCCTGAAG AGGAGGCTGA TCACGCAGCT GCAGCCAGAC 
ACGGACTACT CTTTTGTGCT GACAAGCAGG GGCAACATTG CTGGTGGTCT ACAGCAGCAG 
GTGTCCATCC GCACCGCTCC CGACCTGATC AACGCCAAGC CCAGCACACA TCAGCCTCAG 
GGTAGCAAGA TGACAATCAA CCTGCCCAAG GTCCAGACCA CCACGCGTGT CAGGTGGTAC 
TACATCGTGG TGGTGCCGGT GTCCCAGTCA ACGCAGGGGT GGAAGAATCC AGACGAGATG 
GACCTGGACG AGCTGTTCAA GTCAAGTGAA GGCCCCGTCC TGAGGCGCAG ACGTCACCTG 
GATTCCTCCA AACCTTATAT CGCTGCCAAG TTGGCCTCGT TGCCGACCAC CTTCACCCTG 
GGTGATGAGA AGAGATACAA CGGCTTCTAC AACAAGCCCC TGAGCAGTCC GCAGGAGTAT 
CTCTGCTTTG TCCTGGCAGT ACTCCGATCC GAAGGGGCAG ACAGCACTAC TAATTATATG 
ACATTTTCAG CCAGTCCCTA CTCAGACCCG GTCCACGTGC GAAACAGCAT GGCTCAGGGG 
ATGGAGCAGC CTGAAATGTT GTGGGTTATG GGCCCTGTTT TGGCCGTGGT CCTGATTATC 
ATCATCGTCA TCGCCATCCT GCTCTTCAAG AAGAAGCGAG CGTCGCCCCT ACCCAAAGAT 
GAGCACTCGG GCGGGGTGAA GGACTCCCTG CTGGCCAACT CCTCCGACCC TGTGGAGATG 
AGAAGACTTA ACTACCAGAC CCCAGGAATG AGGGAGCACC CGCCCATCCC TGTCGTCGAC 
CTGGCTGACC ACATAGAGCG TCTGAAGGCC AACGACGGCC TACGCTTTTC CCAGGAGTAC 
GAGTCGATTG ATCCAGGGCA GCAGTTTACC TGGGAGAACT CCAACATGGA AGTCAACAAG 
CCAAAGAATC GCTATGCAAA CGTCATCGCC TATGACCACT CCAGAGTGGT TCTCACCTCT 
GTTGACGCCG TTCCAGGCAG TGACTACATC AACGCCAACT ACATCGACGG CTACAGGAAG 
CAGAACGCCT ACATCGCCAC GCAGGGGCCA CTTCCAGAGA CACTGAGCGA CTTCTGGAGG 
ATGGTGTGGG AGCAAAGATC CAACACCATC GTCATGATGA CCAGACTGGA GGAGAAGTCG 
CGGGTGAAGT GTGACCAGTA CTGGCCGTCG CGAGGAACGG AGACCTATGG GATGATCCAG 
GTCACCATGC TGGACACCGT GGAGTTGGCC ACTTACAGCG TCCGTACCTT TGCCCTCTAT 
AAGAATGGCT CCAGTGAGAA GAGAGAGGTG AGACAGTTCC AGTTCATGGC CTGGCCAGAC 
CACGGGGTGC CAGAGTACCC CACCCCAATC CTGGCCTTCC TACGGCGGGT AAAGGCCTGC 
AATCCTCCAG ACGCAGGGCC TATGGTGGTC CACTGCAGCG CGGGTGTGGG CAGGACCGGA 
TGCTTCATCG TGATAGATGC CATGCTGGAG CGCATGAAGC ACGAGAAGTC CGTAGACATC 
TACGGCCACG TGACGTGCAT GCGCGCCCAG AGGAACTACA TGGTCCAGAC CGAGGACCAG 
TACATCTTCA TTCACGAAGC CCTGCTGGAG GCCGCCACGT GCGGCAACAC CGAGGTGCCG 
GCGCGCAACC TGTACGCCCA CATCCAGAAA CTCACCCAGC CCCCGCCCGG CGAGACGGTT 
ACTGCCATGG AGCTGGAATT CAAGAGACTG GCCAACTCCA AAGCCCACAC ATCCCGCTTC 
ATCAGCGCCA ACTTGCCCTG CAACAAGTTC AAGAACCGCC TGGTCAACAT CATGCCTTTC 
GAGTCCACCC GCGTCTGCCT GCAGCCCATC CGTGGCGTCG AGGGCTCCGA CTACATCAAT 
GCCAGCTGCA TCGACGGCTA CAGACAGCAG AAGGCCTACC TGGCCACGCA GGGCCCACTG 
GCAGAGACCA CCGAGGACTT CTGGAGGATG CTGTGGGAGC ACAACTCCAC CATCGTCGTC 
ATGCTCACCA AGCTACGAGA GATGGGCCGG GAGAAGTGCC ATCAGTACTG GCCAGCAGAG 
CGTTCAGCCA GGTACCAGTA CTTTGTGGTG GATCCAATGG CTGAGTACAA CATGCCCCAG 
TACATCCTCA GAGAGTTCAA AGTCACAGAT GCCAGGGACG GCCAGTCCAG AACAATCCGC 
CAATTCCAGT TCACTGACTG GCCCGAGCAA GGAGTGCCAA AAACCGGAGA GGGCTTCATT 
GATTTTATCG GACAAGTGCA CAAAACCAAG GAACAGTTCG GACAGGACGG ACCAATCACT 
GTACACTGCA GTGCTGGGGT GGGCAGGACC GGAGTCTTCA TCACCCTCAG CATCGTGCTG 
GAGAGGATGA GGTATGAAGG AGTTGTCGAC CTCTTCCAGA CAGTGAAGAC ACTTCGCACC 
CAGCGGCCTG CCATGGTGCA GACCGAGGAC CAGTACCAGT TGTGCTACAG GGCAGCATTG 
GAGTACCTGG GGAGCTTTGA CCACTATGCA ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010744756.2
CDS	474..5849
Translation	MVPNIHLAVPFLPRGALLFLILLSCVVFSSLADSMPSFVKSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVEKTRTATMLCAASGNPDPEISWFKDMLPVDISSSNGRIKQLRSGALQIENSEESDQGKYECVAMNSAGTRYSAPANLYVRVRRVPPRFSIPPTNQEVMPGGSVNLTCVAVGSPMPYVKWTMGLVELTKEEEMPMGRNVLEVNNIRESANYTCVAISSLGMIEATAQVTVKALPKPPASLIVTETTATSVTLTWDSGNPEPVSYYMIQYRAKTSDNGFQEVEGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSDPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRVYYSSDLTAPLSAWQKHNTDDSSLTTISGLTPDITYSLRVLGFTSVGDGPPSDILQVKTQQGVPAQPSSFEAEAELDSRIMLTWLWPVQDPITKYELQYWEAGSDNKLHVSFNPAGSYAVDGLKPDTLYRFSLAARSEMGLGVYTQPIEARTAQSMPGAPPRKLEVEAINSTAIRVTWKPPLQGKQHGQIRGYQVIFSRLENGEPRGQPNIMDVALPEAQEAIIAGLHSETTYSVTVAAYTTKGDGARSKAKVITTTGAVPGKPTMMISTTMGNTALIQWQPPKEMVGELMGYQLQYKRTDEENYTSRELRKIDDHYTVTGLHKGATYVFRLSARNRAGIGEAYVKEISTPEDVPSGFPFNLQVTGLTQSSTELTWDPPLVAERNGKIVHYVVVYRDINSQQNSTNRTSETHMTIQGLNPDTTYDIRVQAFTSKGGGPLSPSIQSRTMSNDFPTLNFGVKAVMKTSVLLTWDLPPNYKSQVPFKILYNQQSVEVQGSLKRRLITQLQPDTDYSFVLTSRGNIAGGLQQQVSIRTAPDLINAKPSTHQPQGSKMTINLPKVQTTTRVRWYYIVVVPVSQSTQGWKNPDEMDLDELFKSSEGPVLRRRRHLDSSKPYIAAKLASLPTTFTLGDEKRYNGFYNKPLSSPQEYLCFVLAVLRSEGADSTTNYMTFSASPYSDPVHVRNSMAQGMEQPEMLWVMGPVLAVVLIIIIVIAILLFKKKRASPLPKDEHSGGVKDSLLANSSDPVEMRRLNYQTPGMREHPPIPVVDLADHIERLKANDGLRFSQEYESIDPGQQFTWENSNMEVNKPKNRYANVIAYDHSRVVLTSVDAVPGSDYINANYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRSNTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVTMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRAQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPPPGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYLATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_010746454.3
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010746454.3

ORF Insert Sequence:

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTGGACATC 
AGCAGCAGCA ACGGGCGCAT TAAACAGCTT CGTTCAGGAG CTCTGCAAAT TGAGAACAGC 
GAGGAGTCCG ACCAAGGGAA ATACGAGTGT GTTGCCATGA ACAGCGCTGG GACCCGTTAC 
TCCGCTCCTG CCAATCTCTA CGTACGAGTG CGAAGAGTCC CACCCAGATT CTCCATTCCG 
CCCACCAACC AAGAGGTGAT GCCAGGCGGC AGCGTCAACC TGACATGCGT GGCAGTGGGC 
TCGCCGATGC CCTACGTCAA GTGGACGATG GGCCTGGTGG AGCTGACCAA GGAGGAGGAG 
ATGCCGATGG GACGCAACGT GCTGGAGGTC AACAACATCC GCGAGTCGGC GAACTACACC 
TGCGTGGCCA TCTCCTCTCT GGGCATGATA GAAGCCACCG CTCAGGTCAC CGTCAAAGCT 
TTGCCAAAGC CTCCCGCCTC CCTCATTGTG ACTGAGACCA CAGCCACCAG TGTGACTCTC 
ACCTGGGACT CTGGAAACCC AGAACCTGTC TCCTACTACA TGATCCAATA CAGAGCCAAG 
ACCTCGGACA ACGGCTTCCA GGAAGTGGAA GGTGTGGCAA CTACCCGTTA CAGCATCGGT 
GGACTTAGCC CCTACTCTGA GTATGAGTTT CGTGTCATGG CTGTCAACAA CATCGGCCGG 
GGGCCACCGA GCGACCCCGT GGAGACACGC ACTGGTGAAC AGGCCCCCTC CTCGCCACCT 
CTGCACGTCC AAGCGCGCAT GCTGAGTGCC AGCACAATGC TGGTCCAGTG GGAACCGCCC 
GAGGAACCCA ACGGGCAAAT CAGGGGCTAC CGGGTCTACT ACAGCTCGGA CTTGACGGCT 
CCGCTCAGCG CCTGGCAGAA GCACAACACA GACGACAGCA GCCTGACGAC CATCTCGGGC 
CTGACTCCTG ATATCACCTA CAGCCTCAGG GTGCTGGGCT TCACCTCTGT AGGTGACGGG 
CCACCTTCTG ACATCCTGCA AGTCAAAACC CAACAGGGAG TCCCTGCCCA GCCGTCCAGC 
TTTGAGGCCG AAGCTGAGCT GGACAGTCGC ATCATGCTAA CATGGCTGTG GCCCGTCCAG 
GACCCCATCA CTAAGTATGA GCTGCAGTAC TGGGAGGCCG GCTCAGACAA CAAGTTACAT 
GTGAGCTTCA ACCCAGCGGG CTCCTACGCT GTGGACGGTC TAAAGCCTGA CACCTTGTAC 
CGTTTCTCTC TGGCAGCCCG TTCTGAGATG GGCTTAGGTG TCTACACCCA ACCCATCGAG 
GCTCGCACCG CCCAGTCCAT GCCGGGAGCA CCCCCCAGGA AGCTGGAGGT TGAGGCCATA 
AACTCCACAG CAATCCGGGT CACCTGGAAG CCGCCCCTGC AGGGGAAGCA GCACGGCCAA 
ATCCGGGGCT ATCAAGTCAT CTTCTCTCGG CTTGAAAACG GCGAACCACG AGGCCAGCCT 
AACATCATGG ACGTTGCTTT GCCAGAGGCA CAGGAGGCCA TTATTGCCGG ACTGCACTCT 
GAAACCACCT ACTCGGTCAC CGTGGCAGCA TACACCACCA AAGGAGACGG AGCGCGCAGC 
AAAGCCAAAG TCATCACCAC GACGGGTGCA GTGCCAGGAA AGCCCACCAT GATGATCAGC 
ACCACTATGG GCAACACAGC GCTGATTCAG TGGCAGCCGC CTAAGGAAAT GGTTGGGGAG 
CTGATGGGCT ACCAGCTGCA GTACAAGCGC ACTGACGAGG AAAACTACAC CTCCCGCGAG 
CTGAGAAAGA TCGATGACCA TTACACCGTC ACCGGGCTGC ACAAAGGTGC CACCTATGTC 
TTCCGCCTCA GTGCCCGCAA CCGCGCGGGC ATCGGCGAAG CATACGTGAA AGAGATCAGC 
ACCCCTGAAG ACGTGCCGTC GGGCTTCCCG TTCAACCTGC AGGTCACGGG ACTGACTCAG 
TCCAGCACCG AGCTGACCTG GGACCCTCCG CTGGTGGCCG AAAGGAACGG CAAGATCGTG 
CACTATGTGG TCGTTTACAG GGACATCAAC AGTCAGCAGA ACAGCACCAA CCGGACGAGC 
GAAACGCACA TGACCATCCA GGGCCTTAAT CCCGACACCA CCTACGATAT CCGGGTGCAG 
GCGTTCACCA GTAAGGGCGG AGGACCCCTC AGCCCTAGCA TCCAGAGCAG GACCATGTCG 
AACGATTTTC CCACCCTCAA CTTTGGTGTC AAAGCCGTCA TGAAAACATC TGTCCTCCTC 
ACCTGGGATT TGCCACCGAA CTACAAATCC CAAGTGCCTT TCAAGATCCT GTACAACCAG 
CAAAGCGTGG AAGTGCAGGG ATCCCTGAAG AGGAGGCTGA TCACGCAGCT GCAGCCAGAC 
ACGGACTACT CTTTTGTGCT GACAAGCAGG GGCAACATTG CTGGTGGTCT ACAGCAGCAG 
GTGTCCATCC GCACCGCTCC CGACCTGATC AACGCCAAGC CCAGCACACA TCAGCCTCAG 
GGTAGCAAGA TGACAATCAA CCTGCCCAAG GTCCAGACCA CCACGCGTGT CAGGTGGTAC 
TACATCGTGG TGGTGCCGGT GTCCCAGTCA ACGCAGGGGT GGAAGAATCC AGACGAGATG 
GACCTGGACG AGCTGTTCAA GTCAAGTGAA GGCCCCGTCC TGAGGCGCAG ACGTCACCTG 
GATTCCTCCA AACCTTATAT CGCTGCCAAG TTGGCCTCGT TGCCGACCAC CTTCACCCTG 
GGTGATGAGA AGAGATACAA CGGCTTCTAC AACAAGCCCC TGAGCAGTCC GCAGGAGTAT 
CTCTGCTTTG TCCTGGCAGT ACTCCGATCC GAAGGGGCAG ACAGCACTAC TAATTATATG 
ACATTTTCAG CCAGTCCCTA CTCAGACCCG GTCCACGTGC GAAACAGCAT GGCTCAGGGG 
ATGGAGCAGC CTGAAATGTT GTGGGTTATG GGCCCTGTTT TGGCCGTGGT CCTGATTATC 
ATCATCGTCA TCGCCATCCT GCTCTTCAAG AAGAAGCGAG CGTCGCCCCT ACCCAAAGAT 
GAGCACTCGG GCGGGGTGAA GGACTCCCTG CTGGCCAACT CCTCCGACCC TGTGGAGATG 
AGAAGACTTA ACTACCAGAC CCCAGGAATG AGGGAGCACC CGCCCATCCC TGTCGTCGAC 
CTGGCTGACC ACATAGAGCG TCTGAAGGCC AACGACGGCC TACGCTTTTC CCAGGAGTAC 
GAGTCGATTG ATCCAGGGCA GCAGTTTACC TGGGAGAACT CCAACATGGA AGTCAACAAG 
CCAAAGAATC GCTATGCAAA CGTCATCGCC TATGACCACT CCAGAGTGGT TCTCACCTCT 
GTTGACGCCG TTCCAGGCAG TGACTACATC AACGCCAACT ACATCGACGG CTACAGGAAG 
CAGAACGCCT ACATCGCCAC GCAGGGGCCA CTTCCAGAGA CACTGAGCGA CTTCTGGAGG 
ATGGTGTGGG AGCAAAGATC CAACACCATC GTCATGATGA CCAGACTGGA GGAGAAGTCG 
CGGGTGAAGT GTGACCAGTA CTGGCCGTCG CGAGGAACGG AGACCTATGG GATGATCCAG 
GTCACCATGC TGGACACCGT GGAGTTGGCC ACTTACAGCG TCCGTACCTT TGCCCTCTAT 
AAGAATGGCT CCAGTGAGAA GAGAGAGGTG AGACAGTTCC AGTTCATGGC CTGGCCAGAC 
CACGGGGTGC CAGAGTACCC CACCCCAATC CTGGCCTTCC TACGGCGGGT AAAGGCCTGC 
AATCCTCCAG ACGCAGGGCC TATGGTGGTC CACTGCAGCG CGGGTGTGGG CAGGACCGGA 
TGCTTCATCG TGATAGATGC CATGCTGGAG CGCATGAAGC ACGAGAAGTC CGTAGACATC 
TACGGCCACG TGACGTGCAT GCGCGCCCAG AGGAACTACA TGGTCCAGAC CGAGGACCAG 
TACATCTTCA TTCACGAAGC CCTGCTGGAG GCCGCCACGT GCGGCAACAC CGAGGTGCCG 
GCGCGCAACC TGTACGCCCA CATCCAGAAA CTCACCCAGC CCCCGCCCGG CGAGACGGTT 
ACTGCCATGG AGCTGGAATT CAAGAGACTG GCCAACTCCA AAGCCCACAC ATCCCGCTTC 
ATCAGCGCCA ACTTGCCCTG CAACAAGTTC AAGAACCGCC TGGTCAACAT CATGCCTTTC 
GAGTCCACCC GCGTCTGCCT GCAGCCCATC CGTGGCGTCG AGGGCTCCGA CTACATCAAT 
GCCAGCTGCA TCGACGGCTA CAGACAGCAG AAGGCCTACC TGGCCACGCA GGGCCCACTG 
GCAGAGACCA CCGAGGACTT CTGGAGGATG CTGTGGGAGC ACAACTCCAC CATCGTCGTC 
ATGCTCACCA AGCTACGAGA GATGGGCCGG GAGAAGTGCC ATCAGTACTG GCCAGCAGAG 
CGTTCAGCCA GGTACCAGTA CTTTGTGGTG GATCCAATGG CTGAGTACAA CATGCCCCAG 
TACATCCTCA GAGAGTTCAA AGTCACAGAT GCCAGGGACG GCCAGTCCAG AACAATCCGC 
CAATTCCAGT TCACTGACTG GCCCGAGCAA GGAGTGCCAA AAACCGGAGA GGGCTTCATT 
GATTTTATCG GACAAGTGCA CAAAACCAAG GAACAGTTCG GACAGGACGG ACCAATCACT 
GTACACTGCA GTGCTGGGGT GGGCAGGACC GGAGTCTTCA TCACCCTCAG CATCGTGCTG 
GAGAGGATGA GGTATGAAGG AGTTGTCGAC CTCTTCCAGA CAGTGAAGAC ACTTCGCACC 
CAGCGGCCTG CCATGGTGCA GACCGAGGAC CAGTACCAGT TGTGCTACAG GGCAGCATTG 
GAGTACCTGG GGAGCTTTGA CCACTATGCA ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104931439 ( XM_019263588.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019119133.1 Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X15, mRNA.

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CloneID	OLa211359
Clone ID Related Accession (Same CDS sequence)	XM_019263588.2
Accession Version	XM_019263588.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	receptor-type tyrosine-protein phosphatase F isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040012.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_019263588.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTGGACATC 
AGCAGCAGCA ACGGGCGCAT TAAACAGCTT CGTTCAGGTG GTACACCAAT TAGAGGAGCT 
CTGCAAATTG AGAACAGCGA GGAGTCCGAC CAAGGGAAAT ACGAGTGTGT TGCCATGAAC 
AGCGCTGGGA CCCGTTACTC CGCTCCTGCC AATCTCTACG TACGAGTGCG AAGAGTCCCA 
CCCAGATTCT CCATTCCGCC CACCAACCAA GAGGTGATGC CAGGCGGCAG CGTCAACCTG 
ACATGCGTGG CAGTGGGCTC GCCGATGCCC TACGTCAAGT GGACGATGGG CCTGGTGGAG 
CTGACCAAGG AGGAGGAGAT GCCGATGGGA CGCAACGTGC TGGAGGTCAA CAACATCCGC 
GAGTCGGCGA ACTACACCTG CGTGGCCATC TCCTCTCTGG GCATGATAGA AGCCACCGCT 
CAGGTCACCG TCAAAGCTTT GCCAAAGCCT CCCGCCTCCC TCATTGTGAC TGAGACCACA 
GCCACCAGTG TGACTCTCAC CTGGGACTCT GGAAACCCAG AACCTGTCTC CTACTACATG 
ATCCAATACA GAGCCAAGAC CTCGGACAAC GGCTTCCAGG AAGTGGAAGG TGTGGCAACT 
ACCCGTTACA GCATCGGTGG ACTTAGCCCC TACTCTGAGT ATGAGTTTCG TGTCATGGCT 
GTCAACAACA TCGGCCGGGG GCCACCGAGC GACCCCGTGG AGACACGCAC TGGTGAACAG 
GCCCCCTCCT CGCCACCTCT GCACGTCCAA GCGCGCATGC TGAGTGCCAG CACAATGCTG 
GTCCAGTGGG AACCGCCCGA GGAACCCAAC GGGCAAATCA GGGGCTACCG GGTCTACTAC 
AGCTCGGACT TGACGGCTCC GCTCAGCGCC TGGCAGAAGC ACAACACAGA CGACAGCAGC 
CTGACGACCA TCTCGGGCCT GACTCCTGAT ATCACCTACA GCCTCAGGGT GCTGGGCTTC 
ACCTCTGTAG GTGACGGGCC ACCTTCTGAC ATCCTGCAAG TCAAAACCCA ACAGGGAGTC 
CCTGCCCAGC CGTCCAGCTT TGAGGCCGAA GCTGAGCTGG ACAGTCGCAT CATGCTAACA 
TGGCTGTGGC CCGTCCAGGA CCCCATCACT AAGTATGAGC TGCAGTACTG GGAGGCCGGC 
TCAGACAACA AGTTACATGT GAGCTTCAAC CCAGCGGGCT CCTACGCTGT GGACGGTCTA 
AAGCCTGACA CCTTGTACCG TTTCTCTCTG GCAGCCCGTT CTGAGATGGG CTTAGGTGTC 
TACACCCAAC CCATCGAGGC TCGCACCGCC CAGTCCATGC CGGGAGCACC CCCCAGGAAG 
CTGGAGGTTG AGGCCATAAA CTCCACAGCA ATCCGGGTCA CCTGGAAGCC GCCCCTGCAG 
GGGAAGCAGC ACGGCCAAAT CCGGGGCTAT CAAGTCATCT TCTCTCGGCT TGAAAACGGC 
GAACCACGAG GCCAGCCTAA CATCATGGAC GTTGCTTTGC CAGAGGCACA GTGGAATATT 
GAGGAGTCCA CCGAGCATGA GGCCATTATT GCCGGACTGC ACTCTGAAAC CACCTACTCG 
GTCACCGTGG CAGCATACAC CACCAAAGGA GACGGAGCGC GCAGCAAAGC CAAAGTCATC 
ACCACGACGG GTGCAGTGCC AGGAAAGCCC ACCATGATGA TCAGCACCAC TATGGGCAAC 
ACAGCGCTGA TTCAGTGGCA GCCGCCTAAG GAAATGGTTG GGGAGCTGAT GGGCTACCAG 
CTGCAGTACA AGCGCACTGA CGAGGAAAAC TACACCTCCC GCGAGCTGAG AAAGATCGAT 
GACCATTACA CCGTCACCGG GCTGCACAAA GGTGCCACCT ATGTCTTCCG CCTCAGTGCC 
CGCAACCGCG CGGGCATCGG CGAAGCATAC GTGAAAGAGA TCAGCACCCC TGAAGACGTG 
CCGTCGGGCT TCCCGTTCAA CCTGCAGGTC ACGGGACTGA CTCAGTCCAG CACCGAGCTG 
ACCTGGGACC CTCCGCTGGT GGCCGAAAGG AACGGCAAGA TCGTGCACTA TGTGGTCGTT 
TACAGGGACA TCAACAGTCA GCAGAACAGC ACCAACCGGA CGAGCGAAAC GCACATGACC 
ATCCAGGGCC TTAATCCCGA CACCACCTAC GATATCCGGG TGCAGGCGTT CACCAGTAAG 
GGCGGAGGAC CCCTCAGCCC TAGCATCCAG AGCAGGACCA TGTCGAACGA TTTTCCCACC 
CTCAACTTTG GTGTCAAAGC CGTCATGAAA ACATCTGTCC TCCTCACCTG GGATTTGCCA 
CCGAACTACA AATCCCAAGT GCCTTTCAAG ATCCTGTACA ACCAGCAAAG CGTGGAAGTG 
CAGGGATCCC TGAAGAGGAG GCTGATCACG CAGCTGCAGC CAGACACGGA CTACTCTTTT 
GTGCTGACAA GCAGGGGCAA CATTGCTGGT GGTCTACAGC AGCAGGTGTC CATCCGCACC 
GCTCCCGACC TGATCAACGC CAAGCCCAGC ACACATCAGC CTCAGGGTAG CAAGATGACA 
ATCAACCTGC CCAAGGTCCA GACCACCACG CGTGTCAGGT GGTACTACAT CGTGGTGGTG 
CCGGTGTCCC AGTCAACGCA GGGGTGGAAG AATCCAGACG AGATGGACCT GGACGAGCTG 
TTCAAGTCAA GTGAAGGCCC CGTCCTGAGG CGCAGACGTC ACCTGGATTC CTCCAAACCT 
TATATCGCTG CCAAGTTGGC CTCGTTGCCG ACCACCTTCA CCCTGGGTGA TGAGAAGAGA 
TACAACGGCT TCTACAACAA GCCCCTGAGC AGTCCGCAGG AGTATCTCTG CTTTGTCCTG 
GCAGTACTCC GATCCGAAGG GGCAGACAGC ACTACTAATT ATATGACATT TTCAGCCAGT 
CCCTACTCAG ACCCGGTCCA CGTGCGAAAC AGCATGGCTC AGGGGATGGA GCAGCCTGAA 
ATGTTGTGGG TTATGGGCCC TGTTTTGGCC GTGGTCCTGA TTATCATCAT CGTCATCGCC 
ATCCTGCTCT TCAAGAAGAA GCGAGCGTCG CCCCTACCCA AAGATGAGCA CTCGGGCGGG 
GTGAAGGACT CCCTGCTGGC CAACTCCTCC GACCCTGTGG AGATGAGAAG ACTTAACTAC 
CAGACCCCAG GTCCCAGCTC TCATCGCTGC CCCAACACTC CAAGAATGAG GGAGCACCCG 
CCCATCCCTG TCGTCGACCT GGCTGACCAC ATAGAGCGTC TGAAGGCCAA CGACGGCCTA 
CGCTTTTCCC AGGAGTACGA GTCGATTGAT CCAGGGCAGC AGTTTACCTG GGAGAACTCC 
AACATGGAAG TCAACAAGCC AAAGAATCGC TATGCAAACG TCATCGCCTA TGACCACTCC 
AGAGTGGTTC TCACCTCTGT TGACGCCGTT CCAGGCAGTG ACTACATCAA CGCCAACTAC 
ATCGACGGCT ACAGGAAGCA GAACGCCTAC ATCGCCACGC AGGGGCCACT TCCAGAGACA 
CTGAGCGACT TCTGGAGGAT GGTGTGGGAG CAAAGATCCA ACACCATCGT CATGATGACC 
AGACTGGAGG AGAAGTCGCG GGTGAAGTGT GACCAGTACT GGCCGTCGCG AGGAACGGAG 
ACCTATGGGA TGATCCAGGT CACCATGCTG GACACCGTGG AGTTGGCCAC TTACAGCGTC 
CGTACCTTTG CCCTCTATAA GAATGGCTCC AGTGAGAAGA GAGAGGTGAG ACAGTTCCAG 
TTCATGGCCT GGCCAGACCA CGGGGTGCCA GAGTACCCCA CCCCAATCCT GGCCTTCCTA 
CGGCGGGTAA AGGCCTGCAA TCCTCCAGAC GCAGGGCCTA TGGTGGTCCA CTGCAGCGCG 
GGTGTGGGCA GGACCGGATG CTTCATCGTG ATAGATGCCA TGCTGGAGCG CATGAAGCAC 
GAGAAGTCCG TAGACATCTA CGGCCACGTG ACGTGCATGC GCGCCCAGAG GAACTACATG 
GTCCAGACCG AGGACCAGTA CATCTTCATT CACGAAGCCC TGCTGGAGGC CGCCACGTGC 
GGCAACACCG AGGTGCCGGC GCGCAACCTG TACGCCCACA TCCAGAAACT CACCCAGCCC 
CCGCCCGGCG AGACGGTTAC TGCCATGGAG CTGGAATTCA AGAGACTGGC CAACTCCAAA 
GCCCACACAT CCCGCTTCAT CAGCGCCAAC TTGCCCTGCA ACAAGTTCAA GAACCGCCTG 
GTCAACATCA TGCCTTTCGA GTCCACCCGC GTCTGCCTGC AGCCCATCCG TGGCGTCGAG 
GGCTCCGACT ACATCAATGC CAGCTGCATC GACGGCTACA GACAGCAGAA GGCCTACCTG 
GCCACGCAGG GCCCACTGGC AGAGACCACC GAGGACTTCT GGAGGATGCT GTGGGAGCAC 
AACTCCACCA TCGTCGTCAT GCTCACCAAG CTACGAGAGA TGGGCCGGGA GAAGTGCCAT 
CAGTACTGGC CAGCAGAGCG TTCAGCCAGG TACCAGTACT TTGTGGTGGA TCCAATGGCT 
GAGTACAACA TGCCCCAGTA CATCCTCAGA GAGTTCAAAG TCACAGATGC CAGGGACGGC 
CAGTCCAGAA CAATCCGCCA ATTCCAGTTC ACTGACTGGC CCGAGCAAGG AGTGCCAAAA 
ACCGGAGAGG GCTTCATTGA TTTTATCGGA CAAGTGCACA AAACCAAGGA ACAGTTCGGA 
CAGGACGGAC CAATCACTGT ACACTGCAGT GCTGGGGTGG GCAGGACCGG AGTCTTCATC 
ACCCTCAGCA TCGTGCTGGA GAGGATGAGG TATGAAGGAG TTGTCGACCT CTTCCAGACA 
GTGAAGACAC TTCGCACCCA GCGGCCTGCC ATGGTGCAGA CCGAGGACCA GTACCAGTTG 
TGCTACAGGG CAGCATTGGA GTACCTGGGG AGCTTTGACC ACTATGCAAC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019119133.1
CDS	481..5934
Translation	MVPNIHLAVPFLPRGALLFLILLSCVVFSSLADSMPSFVKSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVEKTRTATMLCAASGNPDPEISWFKDMLPVDISSSNGRIKQLRSGGTPIRGALQIENSEESDQGKYECVAMNSAGTRYSAPANLYVRVRRVPPRFSIPPTNQEVMPGGSVNLTCVAVGSPMPYVKWTMGLVELTKEEEMPMGRNVLEVNNIRESANYTCVAISSLGMIEATAQVTVKALPKPPASLIVTETTATSVTLTWDSGNPEPVSYYMIQYRAKTSDNGFQEVEGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSDPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRVYYSSDLTAPLSAWQKHNTDDSSLTTISGLTPDITYSLRVLGFTSVGDGPPSDILQVKTQQGVPAQPSSFEAEAELDSRIMLTWLWPVQDPITKYELQYWEAGSDNKLHVSFNPAGSYAVDGLKPDTLYRFSLAARSEMGLGVYTQPIEARTAQSMPGAPPRKLEVEAINSTAIRVTWKPPLQGKQHGQIRGYQVIFSRLENGEPRGQPNIMDVALPEAQWNIEESTEHEAIIAGLHSETTYSVTVAAYTTKGDGARSKAKVITTTGAVPGKPTMMISTTMGNTALIQWQPPKEMVGELMGYQLQYKRTDEENYTSRELRKIDDHYTVTGLHKGATYVFRLSARNRAGIGEAYVKEISTPEDVPSGFPFNLQVTGLTQSSTELTWDPPLVAERNGKIVHYVVVYRDINSQQNSTNRTSETHMTIQGLNPDTTYDIRVQAFTSKGGGPLSPSIQSRTMSNDFPTLNFGVKAVMKTSVLLTWDLPPNYKSQVPFKILYNQQSVEVQGSLKRRLITQLQPDTDYSFVLTSRGNIAGGLQQQVSIRTAPDLINAKPSTHQPQGSKMTINLPKVQTTTRVRWYYIVVVPVSQSTQGWKNPDEMDLDELFKSSEGPVLRRRRHLDSSKPYIAAKLASLPTTFTLGDEKRYNGFYNKPLSSPQEYLCFVLAVLRSEGADSTTNYMTFSASPYSDPVHVRNSMAQGMEQPEMLWVMGPVLAVVLIIIIVIAILLFKKKRASPLPKDEHSGGVKDSLLANSSDPVEMRRLNYQTPGPSSHRCPNTPRMREHPPIPVVDLADHIERLKANDGLRFSQEYESIDPGQQFTWENSNMEVNKPKNRYANVIAYDHSRVVLTSVDAVPGSDYINANYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRSNTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVTMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRAQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPPPGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYLATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_019263588.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019263588.2

ORF Insert Sequence:

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTGGACATC 
AGCAGCAGCA ACGGGCGCAT TAAACAGCTT CGTTCAGGTG GTACACCAAT TAGAGGAGCT 
CTGCAAATTG AGAACAGCGA GGAGTCCGAC CAAGGGAAAT ACGAGTGTGT TGCCATGAAC 
AGCGCTGGGA CCCGTTACTC CGCTCCTGCC AATCTCTACG TACGAGTGCG AAGAGTCCCA 
CCCAGATTCT CCATTCCGCC CACCAACCAA GAGGTGATGC CAGGCGGCAG CGTCAACCTG 
ACATGCGTGG CAGTGGGCTC GCCGATGCCC TACGTCAAGT GGACGATGGG CCTGGTGGAG 
CTGACCAAGG AGGAGGAGAT GCCGATGGGA CGCAACGTGC TGGAGGTCAA CAACATCCGC 
GAGTCGGCGA ACTACACCTG CGTGGCCATC TCCTCTCTGG GCATGATAGA AGCCACCGCT 
CAGGTCACCG TCAAAGCTTT GCCAAAGCCT CCCGCCTCCC TCATTGTGAC TGAGACCACA 
GCCACCAGTG TGACTCTCAC CTGGGACTCT GGAAACCCAG AACCTGTCTC CTACTACATG 
ATCCAATACA GAGCCAAGAC CTCGGACAAC GGCTTCCAGG AAGTGGAAGG TGTGGCAACT 
ACCCGTTACA GCATCGGTGG ACTTAGCCCC TACTCTGAGT ATGAGTTTCG TGTCATGGCT 
GTCAACAACA TCGGCCGGGG GCCACCGAGC GACCCCGTGG AGACACGCAC TGGTGAACAG 
GCCCCCTCCT CGCCACCTCT GCACGTCCAA GCGCGCATGC TGAGTGCCAG CACAATGCTG 
GTCCAGTGGG AACCGCCCGA GGAACCCAAC GGGCAAATCA GGGGCTACCG GGTCTACTAC 
AGCTCGGACT TGACGGCTCC GCTCAGCGCC TGGCAGAAGC ACAACACAGA CGACAGCAGC 
CTGACGACCA TCTCGGGCCT GACTCCTGAT ATCACCTACA GCCTCAGGGT GCTGGGCTTC 
ACCTCTGTAG GTGACGGGCC ACCTTCTGAC ATCCTGCAAG TCAAAACCCA ACAGGGAGTC 
CCTGCCCAGC CGTCCAGCTT TGAGGCCGAA GCTGAGCTGG ACAGTCGCAT CATGCTAACA 
TGGCTGTGGC CCGTCCAGGA CCCCATCACT AAGTATGAGC TGCAGTACTG GGAGGCCGGC 
TCAGACAACA AGTTACATGT GAGCTTCAAC CCAGCGGGCT CCTACGCTGT GGACGGTCTA 
AAGCCTGACA CCTTGTACCG TTTCTCTCTG GCAGCCCGTT CTGAGATGGG CTTAGGTGTC 
TACACCCAAC CCATCGAGGC TCGCACCGCC CAGTCCATGC CGGGAGCACC CCCCAGGAAG 
CTGGAGGTTG AGGCCATAAA CTCCACAGCA ATCCGGGTCA CCTGGAAGCC GCCCCTGCAG 
GGGAAGCAGC ACGGCCAAAT CCGGGGCTAT CAAGTCATCT TCTCTCGGCT TGAAAACGGC 
GAACCACGAG GCCAGCCTAA CATCATGGAC GTTGCTTTGC CAGAGGCACA GTGGAATATT 
GAGGAGTCCA CCGAGCATGA GGCCATTATT GCCGGACTGC ACTCTGAAAC CACCTACTCG 
GTCACCGTGG CAGCATACAC CACCAAAGGA GACGGAGCGC GCAGCAAAGC CAAAGTCATC 
ACCACGACGG GTGCAGTGCC AGGAAAGCCC ACCATGATGA TCAGCACCAC TATGGGCAAC 
ACAGCGCTGA TTCAGTGGCA GCCGCCTAAG GAAATGGTTG GGGAGCTGAT GGGCTACCAG 
CTGCAGTACA AGCGCACTGA CGAGGAAAAC TACACCTCCC GCGAGCTGAG AAAGATCGAT 
GACCATTACA CCGTCACCGG GCTGCACAAA GGTGCCACCT ATGTCTTCCG CCTCAGTGCC 
CGCAACCGCG CGGGCATCGG CGAAGCATAC GTGAAAGAGA TCAGCACCCC TGAAGACGTG 
CCGTCGGGCT TCCCGTTCAA CCTGCAGGTC ACGGGACTGA CTCAGTCCAG CACCGAGCTG 
ACCTGGGACC CTCCGCTGGT GGCCGAAAGG AACGGCAAGA TCGTGCACTA TGTGGTCGTT 
TACAGGGACA TCAACAGTCA GCAGAACAGC ACCAACCGGA CGAGCGAAAC GCACATGACC 
ATCCAGGGCC TTAATCCCGA CACCACCTAC GATATCCGGG TGCAGGCGTT CACCAGTAAG 
GGCGGAGGAC CCCTCAGCCC TAGCATCCAG AGCAGGACCA TGTCGAACGA TTTTCCCACC 
CTCAACTTTG GTGTCAAAGC CGTCATGAAA ACATCTGTCC TCCTCACCTG GGATTTGCCA 
CCGAACTACA AATCCCAAGT GCCTTTCAAG ATCCTGTACA ACCAGCAAAG CGTGGAAGTG 
CAGGGATCCC TGAAGAGGAG GCTGATCACG CAGCTGCAGC CAGACACGGA CTACTCTTTT 
GTGCTGACAA GCAGGGGCAA CATTGCTGGT GGTCTACAGC AGCAGGTGTC CATCCGCACC 
GCTCCCGACC TGATCAACGC CAAGCCCAGC ACACATCAGC CTCAGGGTAG CAAGATGACA 
ATCAACCTGC CCAAGGTCCA GACCACCACG CGTGTCAGGT GGTACTACAT CGTGGTGGTG 
CCGGTGTCCC AGTCAACGCA GGGGTGGAAG AATCCAGACG AGATGGACCT GGACGAGCTG 
TTCAAGTCAA GTGAAGGCCC CGTCCTGAGG CGCAGACGTC ACCTGGATTC CTCCAAACCT 
TATATCGCTG CCAAGTTGGC CTCGTTGCCG ACCACCTTCA CCCTGGGTGA TGAGAAGAGA 
TACAACGGCT TCTACAACAA GCCCCTGAGC AGTCCGCAGG AGTATCTCTG CTTTGTCCTG 
GCAGTACTCC GATCCGAAGG GGCAGACAGC ACTACTAATT ATATGACATT TTCAGCCAGT 
CCCTACTCAG ACCCGGTCCA CGTGCGAAAC AGCATGGCTC AGGGGATGGA GCAGCCTGAA 
ATGTTGTGGG TTATGGGCCC TGTTTTGGCC GTGGTCCTGA TTATCATCAT CGTCATCGCC 
ATCCTGCTCT TCAAGAAGAA GCGAGCGTCG CCCCTACCCA AAGATGAGCA CTCGGGCGGG 
GTGAAGGACT CCCTGCTGGC CAACTCCTCC GACCCTGTGG AGATGAGAAG ACTTAACTAC 
CAGACCCCAG GTCCCAGCTC TCATCGCTGC CCCAACACTC CAAGAATGAG GGAGCACCCG 
CCCATCCCTG TCGTCGACCT GGCTGACCAC ATAGAGCGTC TGAAGGCCAA CGACGGCCTA 
CGCTTTTCCC AGGAGTACGA GTCGATTGAT CCAGGGCAGC AGTTTACCTG GGAGAACTCC 
AACATGGAAG TCAACAAGCC AAAGAATCGC TATGCAAACG TCATCGCCTA TGACCACTCC 
AGAGTGGTTC TCACCTCTGT TGACGCCGTT CCAGGCAGTG ACTACATCAA CGCCAACTAC 
ATCGACGGCT ACAGGAAGCA GAACGCCTAC ATCGCCACGC AGGGGCCACT TCCAGAGACA 
CTGAGCGACT TCTGGAGGAT GGTGTGGGAG CAAAGATCCA ACACCATCGT CATGATGACC 
AGACTGGAGG AGAAGTCGCG GGTGAAGTGT GACCAGTACT GGCCGTCGCG AGGAACGGAG 
ACCTATGGGA TGATCCAGGT CACCATGCTG GACACCGTGG AGTTGGCCAC TTACAGCGTC 
CGTACCTTTG CCCTCTATAA GAATGGCTCC AGTGAGAAGA GAGAGGTGAG ACAGTTCCAG 
TTCATGGCCT GGCCAGACCA CGGGGTGCCA GAGTACCCCA CCCCAATCCT GGCCTTCCTA 
CGGCGGGTAA AGGCCTGCAA TCCTCCAGAC GCAGGGCCTA TGGTGGTCCA CTGCAGCGCG 
GGTGTGGGCA GGACCGGATG CTTCATCGTG ATAGATGCCA TGCTGGAGCG CATGAAGCAC 
GAGAAGTCCG TAGACATCTA CGGCCACGTG ACGTGCATGC GCGCCCAGAG GAACTACATG 
GTCCAGACCG AGGACCAGTA CATCTTCATT CACGAAGCCC TGCTGGAGGC CGCCACGTGC 
GGCAACACCG AGGTGCCGGC GCGCAACCTG TACGCCCACA TCCAGAAACT CACCCAGCCC 
CCGCCCGGCG AGACGGTTAC TGCCATGGAG CTGGAATTCA AGAGACTGGC CAACTCCAAA 
GCCCACACAT CCCGCTTCAT CAGCGCCAAC TTGCCCTGCA ACAAGTTCAA GAACCGCCTG 
GTCAACATCA TGCCTTTCGA GTCCACCCGC GTCTGCCTGC AGCCCATCCG TGGCGTCGAG 
GGCTCCGACT ACATCAATGC CAGCTGCATC GACGGCTACA GACAGCAGAA GGCCTACCTG 
GCCACGCAGG GCCCACTGGC AGAGACCACC GAGGACTTCT GGAGGATGCT GTGGGAGCAC 
AACTCCACCA TCGTCGTCAT GCTCACCAAG CTACGAGAGA TGGGCCGGGA GAAGTGCCAT 
CAGTACTGGC CAGCAGAGCG TTCAGCCAGG TACCAGTACT TTGTGGTGGA TCCAATGGCT 
GAGTACAACA TGCCCCAGTA CATCCTCAGA GAGTTCAAAG TCACAGATGC CAGGGACGGC 
CAGTCCAGAA CAATCCGCCA ATTCCAGTTC ACTGACTGGC CCGAGCAAGG AGTGCCAAAA 
ACCGGAGAGG GCTTCATTGA TTTTATCGGA CAAGTGCACA AAACCAAGGA ACAGTTCGGA 
CAGGACGGAC CAATCACTGT ACACTGCAGT GCTGGGGTGG GCAGGACCGG AGTCTTCATC 
ACCCTCAGCA TCGTGCTGGA GAGGATGAGG TATGAAGGAG TTGTCGACCT CTTCCAGACA 
GTGAAGACAC TTCGCACCCA GCGGCCTGCC ATGGTGCAGA CCGAGGACCA GTACCAGTTG 
TGCTACAGGG CAGCATTGGA GTACCTGGGG AGCTTTGACC ACTATGCAAC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104931439 ( XM_019263593.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019119138.1 Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X16, mRNA.

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CloneID	OLa211360
Clone ID Related Accession (Same CDS sequence)	XM_019263593.2
Accession Version	XM_019263593.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	receptor-type tyrosine-protein phosphatase F isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040012.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_019263593.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTGGACATC 
AGCAGCAGCA ACGGGCGCAT TAAACAGCTT CGTTCAGGAG CTCTGCAAAT TGAGAACAGC 
GAGGAGTCCG ACCAAGGGAA ATACGAGTGT GTTGCCATGA ACAGCGCTGG GACCCGTTAC 
TCCGCTCCTG CCAATCTCTA CGTACGAGTG CGAAGAGTCC CACCCAGATT CTCCATTCCG 
CCCACCAACC AAGAGGTGAT GCCAGGCGGC AGCGTCAACC TGACATGCGT GGCAGTGGGC 
TCGCCGATGC CCTACGTCAA GTGGACGATG GGCCTGGTGG AGCTGACCAA GGAGGAGGAG 
ATGCCGATGG GACGCAACGT GCTGGAGGTC AACAACATCC GCGAGTCGGC GAACTACACC 
TGCGTGGCCA TCTCCTCTCT GGGCATGATA GAAGCCACCG CTCAGGTCAC CGTCAAAGCT 
TTGCCAAAGC CTCCCGCCTC CCTCATTGTG ACTGAGACCA CAGCCACCAG TGTGACTCTC 
ACCTGGGACT CTGGAAACCC AGAACCTGTC TCCTACTACA TGATCCAATA CAGAGCCAAG 
ACCTCGGACA ACGGCTTCCA GGAAGTGGAA GGTGTGGCAA CTACCCGTTA CAGCATCGGT 
GGACTTAGCC CCTACTCTGA GTATGAGTTT CGTGTCATGG CTGTCAACAA CATCGGCCGG 
GGGCCACCGA GCGACCCCGT GGAGACACGC ACTGGTGAAC AGGCCCCCTC CTCGCCACCT 
CTGCACGTCC AAGCGCGCAT GCTGAGTGCC AGCACAATGC TGGTCCAGTG GGAACCGCCC 
GAGGAACCCA ACGGGCAAAT CAGGGGCTAC CGGGTCTACT ACAGCTCGGA CTTGACGGCT 
CCGCTCAGCG CCTGGCAGAA GCACAACACA GACGACAGCA GCCTGACGAC CATCTCGGGC 
CTGACTCCTG ATATCACCTA CAGCCTCAGG GTGCTGGGCT TCACCTCTGT AGGTGACGGG 
CCACCTTCTG ACATCCTGCA AGTCAAAACC CAACAGGGAG TCCCTGCCCA GCCGTCCAGC 
TTTGAGGCCG AAGCTGAGCT GGACAGTCGC ATCATGCTAA CATGGCTGTG GCCCGTCCAG 
GACCCCATCA CTAAGTATGA GCTGCAGTAC TGGGAGGCCG GCTCAGACAA CAAGTTACAT 
GTGAGCTTCA ACCCAGCGGG CTCCTACGCT GTGGACGGTC TAAAGCCTGA CACCTTGTAC 
CGTTTCTCTC TGGCAGCCCG TTCTGAGATG GGCTTAGGTG TCTACACCCA ACCCATCGAG 
GCTCGCACCG CCCAGTCCAT GCCGGGAGCA CCCCCCAGGA AGCTGGAGGT TGAGGCCATA 
AACTCCACAG CAATCCGGGT CACCTGGAAG CCGCCCCTGC AGGGGAAGCA GCACGGCCAA 
ATCCGGGGCT ATCAAGTCAT CTTCTCTCGG CTTGAAAACG GCGAACCACG AGGCCAGCCT 
AACATCATGG ACGTTGCTTT GCCAGAGGCA CAGTGGAATA TTGAGGAGTC CACCGAGCAT 
GAGGCCATTA TTGCCGGACT GCACTCTGAA ACCACCTACT CGGTCACCGT GGCAGCATAC 
ACCACCAAAG GAGACGGAGC GCGCAGCAAA GCCAAAGTCA TCACCACGAC GGGTGCAGTG 
CCAGGAAAGC CCACCATGAT GATCAGCACC ACTATGGGCA ACACAGCGCT GATTCAGTGG 
CAGCCGCCTA AGGAAATGGT TGGGGAGCTG ATGGGCTACC AGCTGCAGTA CAAGCGCACT 
GACGAGGAAA ACTACACCTC CCGCGAGCTG AGAAAGATCG ATGACCATTA CACCGTCACC 
GGGCTGCACA AAGGTGCCAC CTATGTCTTC CGCCTCAGTG CCCGCAACCG CGCGGGCATC 
GGCGAAGCAT ACGTGAAAGA GATCAGCACC CCTGAAGACG TGCCGTCGGG CTTCCCGTTC 
AACCTGCAGG TCACGGGACT GACTCAGTCC AGCACCGAGC TGACCTGGGA CCCTCCGCTG 
GTGGCCGAAA GGAACGGCAA GATCGTGCAC TATGTGGTCG TTTACAGGGA CATCAACAGT 
CAGCAGAACA GCACCAACCG GACGAGCGAA ACGCACATGA CCATCCAGGG CCTTAATCCC 
GACACCACCT ACGATATCCG GGTGCAGGCG TTCACCAGTA AGGGCGGAGG ACCCCTCAGC 
CCTAGCATCC AGAGCAGGAC CATGTCGAAC GATTTTCCCA CCCTCAACTT TGGTGTCAAA 
GCCGTCATGA AAACATCTGT CCTCCTCACC TGGGATTTGC CACCGAACTA CAAATCCCAA 
GTGCCTTTCA AGATCCTGTA CAACCAGCAA AGCGTGGAAG TGCAGGGATC CCTGAAGAGG 
AGGCTGATCA CGCAGCTGCA GCCAGACACG GACTACTCTT TTGTGCTGAC AAGCAGGGGC 
AACATTGCTG GTGGTCTACA GCAGCAGGTG TCCATCCGCA CCGCTCCCGA CCTGATCAAC 
GCCAAGCCCA GCACACATCA GCCTCAGGGT AGCAAGATGA CAATCAACCT GCCCAAGGTC 
CAGACCACCA CGCGTGTCAG GTGGTACTAC ATCGTGGTGG TGCCGGTGTC CCAGTCAACG 
CAGGGGTGGA AGAATCCAGA CGAGATGGAC CTGGACGAGC TGTTCAAGTC AAGTGAAGGC 
CCCGTCCTGA GGCGCAGACG TCACCTGGAT TCCTCCAAAC CTTATATCGC TGCCAAGTTG 
GCCTCGTTGC CGACCACCTT CACCCTGGGT GATGAGAAGA GATACAACGG CTTCTACAAC 
AAGCCCCTGA GCAGTCCGCA GGAGTATCTC TGCTTTGTCC TGGCAGTACT CCGATCCGAA 
GGGGCAGACA GCACTACTAA TTATATGACA TTTTCAGCCA GTCCCTACTC AGACCCGGTC 
CACGTGCGAA ACAGCATGGC TCAGGGGATG GAGCAGCCTG AAATGTTGTG GGTTATGGGC 
CCTGTTTTGG CCGTGGTCCT GATTATCATC ATCGTCATCG CCATCCTGCT CTTCAAGAAG 
AAGCGAGCGT CGCCCCTACC CAAAGATGAG CACTCGGGCG GGGTGAAGGA CTCCCTGCTG 
GCCAACTCCT CCGACCCTGT GGAGATGAGA AGACTTAACT ACCAGACCCC AGGTCCCAGC 
TCTCATCGCT GCCCCAACAC TCCAAGAATG AGGGAGCACC CGCCCATCCC TGTCGTCGAC 
CTGGCTGACC ACATAGAGCG TCTGAAGGCC AACGACGGCC TACGCTTTTC CCAGGAGTAC 
GAGTCGATTG ATCCAGGGCA GCAGTTTACC TGGGAGAACT CCAACATGGA AGTCAACAAG 
CCAAAGAATC GCTATGCAAA CGTCATCGCC TATGACCACT CCAGAGTGGT TCTCACCTCT 
GTTGACGCCG TTCCAGGCAG TGACTACATC AACGCCAACT ACATCGACGG CTACAGGAAG 
CAGAACGCCT ACATCGCCAC GCAGGGGCCA CTTCCAGAGA CACTGAGCGA CTTCTGGAGG 
ATGGTGTGGG AGCAAAGATC CAACACCATC GTCATGATGA CCAGACTGGA GGAGAAGTCG 
CGGGTGAAGT GTGACCAGTA CTGGCCGTCG CGAGGAACGG AGACCTATGG GATGATCCAG 
GTCACCATGC TGGACACCGT GGAGTTGGCC ACTTACAGCG TCCGTACCTT TGCCCTCTAT 
AAGAATGGCT CCAGTGAGAA GAGAGAGGTG AGACAGTTCC AGTTCATGGC CTGGCCAGAC 
CACGGGGTGC CAGAGTACCC CACCCCAATC CTGGCCTTCC TACGGCGGGT AAAGGCCTGC 
AATCCTCCAG ACGCAGGGCC TATGGTGGTC CACTGCAGCG CGGGTGTGGG CAGGACCGGA 
TGCTTCATCG TGATAGATGC CATGCTGGAG CGCATGAAGC ACGAGAAGTC CGTAGACATC 
TACGGCCACG TGACGTGCAT GCGCGCCCAG AGGAACTACA TGGTCCAGAC CGAGGACCAG 
TACATCTTCA TTCACGAAGC CCTGCTGGAG GCCGCCACGT GCGGCAACAC CGAGGTGCCG 
GCGCGCAACC TGTACGCCCA CATCCAGAAA CTCACCCAGC CCCCGCCCGG CGAGACGGTT 
ACTGCCATGG AGCTGGAATT CAAGAGACTG GCCAACTCCA AAGCCCACAC ATCCCGCTTC 
ATCAGCGCCA ACTTGCCCTG CAACAAGTTC AAGAACCGCC TGGTCAACAT CATGCCTTTC 
GAGTCCACCC GCGTCTGCCT GCAGCCCATC CGTGGCGTCG AGGGCTCCGA CTACATCAAT 
GCCAGCTGCA TCGACGGCTA CAGACAGCAG AAGGCCTACC TGGCCACGCA GGGCCCACTG 
GCAGAGACCA CCGAGGACTT CTGGAGGATG CTGTGGGAGC ACAACTCCAC CATCGTCGTC 
ATGCTCACCA AGCTACGAGA GATGGGCCGG GAGAAGTGCC ATCAGTACTG GCCAGCAGAG 
CGTTCAGCCA GGTACCAGTA CTTTGTGGTG GATCCAATGG CTGAGTACAA CATGCCCCAG 
TACATCCTCA GAGAGTTCAA AGTCACAGAT GCCAGGGACG GCCAGTCCAG AACAATCCGC 
CAATTCCAGT TCACTGACTG GCCCGAGCAA GGAGTGCCAA AAACCGGAGA GGGCTTCATT 
GATTTTATCG GACAAGTGCA CAAAACCAAG GAACAGTTCG GACAGGACGG ACCAATCACT 
GTACACTGCA GTGCTGGGGT GGGCAGGACC GGAGTCTTCA TCACCCTCAG CATCGTGCTG 
GAGAGGATGA GGTATGAAGG AGTTGTCGAC CTCTTCCAGA CAGTGAAGAC ACTTCGCACC 
CAGCGGCCTG CCATGGTGCA GACCGAGGAC CAGTACCAGT TGTGCTACAG GGCAGCATTG 
GAGTACCTGG GGAGCTTTGA CCACTATGCA ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019119138.1
CDS	478..5913
Translation	MVPNIHLAVPFLPRGALLFLILLSCVVFSSLADSMPSFVKSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVEKTRTATMLCAASGNPDPEISWFKDMLPVDISSSNGRIKQLRSGALQIENSEESDQGKYECVAMNSAGTRYSAPANLYVRVRRVPPRFSIPPTNQEVMPGGSVNLTCVAVGSPMPYVKWTMGLVELTKEEEMPMGRNVLEVNNIRESANYTCVAISSLGMIEATAQVTVKALPKPPASLIVTETTATSVTLTWDSGNPEPVSYYMIQYRAKTSDNGFQEVEGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSDPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRVYYSSDLTAPLSAWQKHNTDDSSLTTISGLTPDITYSLRVLGFTSVGDGPPSDILQVKTQQGVPAQPSSFEAEAELDSRIMLTWLWPVQDPITKYELQYWEAGSDNKLHVSFNPAGSYAVDGLKPDTLYRFSLAARSEMGLGVYTQPIEARTAQSMPGAPPRKLEVEAINSTAIRVTWKPPLQGKQHGQIRGYQVIFSRLENGEPRGQPNIMDVALPEAQWNIEESTEHEAIIAGLHSETTYSVTVAAYTTKGDGARSKAKVITTTGAVPGKPTMMISTTMGNTALIQWQPPKEMVGELMGYQLQYKRTDEENYTSRELRKIDDHYTVTGLHKGATYVFRLSARNRAGIGEAYVKEISTPEDVPSGFPFNLQVTGLTQSSTELTWDPPLVAERNGKIVHYVVVYRDINSQQNSTNRTSETHMTIQGLNPDTTYDIRVQAFTSKGGGPLSPSIQSRTMSNDFPTLNFGVKAVMKTSVLLTWDLPPNYKSQVPFKILYNQQSVEVQGSLKRRLITQLQPDTDYSFVLTSRGNIAGGLQQQVSIRTAPDLINAKPSTHQPQGSKMTINLPKVQTTTRVRWYYIVVVPVSQSTQGWKNPDEMDLDELFKSSEGPVLRRRRHLDSSKPYIAAKLASLPTTFTLGDEKRYNGFYNKPLSSPQEYLCFVLAVLRSEGADSTTNYMTFSASPYSDPVHVRNSMAQGMEQPEMLWVMGPVLAVVLIIIIVIAILLFKKKRASPLPKDEHSGGVKDSLLANSSDPVEMRRLNYQTPGPSSHRCPNTPRMREHPPIPVVDLADHIERLKANDGLRFSQEYESIDPGQQFTWENSNMEVNKPKNRYANVIAYDHSRVVLTSVDAVPGSDYINANYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRSNTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVTMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRAQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPPPGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYLATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_019263593.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019263593.2

ORF Insert Sequence:

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTGGACATC 
AGCAGCAGCA ACGGGCGCAT TAAACAGCTT CGTTCAGGAG CTCTGCAAAT TGAGAACAGC 
GAGGAGTCCG ACCAAGGGAA ATACGAGTGT GTTGCCATGA ACAGCGCTGG GACCCGTTAC 
TCCGCTCCTG CCAATCTCTA CGTACGAGTG CGAAGAGTCC CACCCAGATT CTCCATTCCG 
CCCACCAACC AAGAGGTGAT GCCAGGCGGC AGCGTCAACC TGACATGCGT GGCAGTGGGC 
TCGCCGATGC CCTACGTCAA GTGGACGATG GGCCTGGTGG AGCTGACCAA GGAGGAGGAG 
ATGCCGATGG GACGCAACGT GCTGGAGGTC AACAACATCC GCGAGTCGGC GAACTACACC 
TGCGTGGCCA TCTCCTCTCT GGGCATGATA GAAGCCACCG CTCAGGTCAC CGTCAAAGCT 
TTGCCAAAGC CTCCCGCCTC CCTCATTGTG ACTGAGACCA CAGCCACCAG TGTGACTCTC 
ACCTGGGACT CTGGAAACCC AGAACCTGTC TCCTACTACA TGATCCAATA CAGAGCCAAG 
ACCTCGGACA ACGGCTTCCA GGAAGTGGAA GGTGTGGCAA CTACCCGTTA CAGCATCGGT 
GGACTTAGCC CCTACTCTGA GTATGAGTTT CGTGTCATGG CTGTCAACAA CATCGGCCGG 
GGGCCACCGA GCGACCCCGT GGAGACACGC ACTGGTGAAC AGGCCCCCTC CTCGCCACCT 
CTGCACGTCC AAGCGCGCAT GCTGAGTGCC AGCACAATGC TGGTCCAGTG GGAACCGCCC 
GAGGAACCCA ACGGGCAAAT CAGGGGCTAC CGGGTCTACT ACAGCTCGGA CTTGACGGCT 
CCGCTCAGCG CCTGGCAGAA GCACAACACA GACGACAGCA GCCTGACGAC CATCTCGGGC 
CTGACTCCTG ATATCACCTA CAGCCTCAGG GTGCTGGGCT TCACCTCTGT AGGTGACGGG 
CCACCTTCTG ACATCCTGCA AGTCAAAACC CAACAGGGAG TCCCTGCCCA GCCGTCCAGC 
TTTGAGGCCG AAGCTGAGCT GGACAGTCGC ATCATGCTAA CATGGCTGTG GCCCGTCCAG 
GACCCCATCA CTAAGTATGA GCTGCAGTAC TGGGAGGCCG GCTCAGACAA CAAGTTACAT 
GTGAGCTTCA ACCCAGCGGG CTCCTACGCT GTGGACGGTC TAAAGCCTGA CACCTTGTAC 
CGTTTCTCTC TGGCAGCCCG TTCTGAGATG GGCTTAGGTG TCTACACCCA ACCCATCGAG 
GCTCGCACCG CCCAGTCCAT GCCGGGAGCA CCCCCCAGGA AGCTGGAGGT TGAGGCCATA 
AACTCCACAG CAATCCGGGT CACCTGGAAG CCGCCCCTGC AGGGGAAGCA GCACGGCCAA 
ATCCGGGGCT ATCAAGTCAT CTTCTCTCGG CTTGAAAACG GCGAACCACG AGGCCAGCCT 
AACATCATGG ACGTTGCTTT GCCAGAGGCA CAGTGGAATA TTGAGGAGTC CACCGAGCAT 
GAGGCCATTA TTGCCGGACT GCACTCTGAA ACCACCTACT CGGTCACCGT GGCAGCATAC 
ACCACCAAAG GAGACGGAGC GCGCAGCAAA GCCAAAGTCA TCACCACGAC GGGTGCAGTG 
CCAGGAAAGC CCACCATGAT GATCAGCACC ACTATGGGCA ACACAGCGCT GATTCAGTGG 
CAGCCGCCTA AGGAAATGGT TGGGGAGCTG ATGGGCTACC AGCTGCAGTA CAAGCGCACT 
GACGAGGAAA ACTACACCTC CCGCGAGCTG AGAAAGATCG ATGACCATTA CACCGTCACC 
GGGCTGCACA AAGGTGCCAC CTATGTCTTC CGCCTCAGTG CCCGCAACCG CGCGGGCATC 
GGCGAAGCAT ACGTGAAAGA GATCAGCACC CCTGAAGACG TGCCGTCGGG CTTCCCGTTC 
AACCTGCAGG TCACGGGACT GACTCAGTCC AGCACCGAGC TGACCTGGGA CCCTCCGCTG 
GTGGCCGAAA GGAACGGCAA GATCGTGCAC TATGTGGTCG TTTACAGGGA CATCAACAGT 
CAGCAGAACA GCACCAACCG GACGAGCGAA ACGCACATGA CCATCCAGGG CCTTAATCCC 
GACACCACCT ACGATATCCG GGTGCAGGCG TTCACCAGTA AGGGCGGAGG ACCCCTCAGC 
CCTAGCATCC AGAGCAGGAC CATGTCGAAC GATTTTCCCA CCCTCAACTT TGGTGTCAAA 
GCCGTCATGA AAACATCTGT CCTCCTCACC TGGGATTTGC CACCGAACTA CAAATCCCAA 
GTGCCTTTCA AGATCCTGTA CAACCAGCAA AGCGTGGAAG TGCAGGGATC CCTGAAGAGG 
AGGCTGATCA CGCAGCTGCA GCCAGACACG GACTACTCTT TTGTGCTGAC AAGCAGGGGC 
AACATTGCTG GTGGTCTACA GCAGCAGGTG TCCATCCGCA CCGCTCCCGA CCTGATCAAC 
GCCAAGCCCA GCACACATCA GCCTCAGGGT AGCAAGATGA CAATCAACCT GCCCAAGGTC 
CAGACCACCA CGCGTGTCAG GTGGTACTAC ATCGTGGTGG TGCCGGTGTC CCAGTCAACG 
CAGGGGTGGA AGAATCCAGA CGAGATGGAC CTGGACGAGC TGTTCAAGTC AAGTGAAGGC 
CCCGTCCTGA GGCGCAGACG TCACCTGGAT TCCTCCAAAC CTTATATCGC TGCCAAGTTG 
GCCTCGTTGC CGACCACCTT CACCCTGGGT GATGAGAAGA GATACAACGG CTTCTACAAC 
AAGCCCCTGA GCAGTCCGCA GGAGTATCTC TGCTTTGTCC TGGCAGTACT CCGATCCGAA 
GGGGCAGACA GCACTACTAA TTATATGACA TTTTCAGCCA GTCCCTACTC AGACCCGGTC 
CACGTGCGAA ACAGCATGGC TCAGGGGATG GAGCAGCCTG AAATGTTGTG GGTTATGGGC 
CCTGTTTTGG CCGTGGTCCT GATTATCATC ATCGTCATCG CCATCCTGCT CTTCAAGAAG 
AAGCGAGCGT CGCCCCTACC CAAAGATGAG CACTCGGGCG GGGTGAAGGA CTCCCTGCTG 
GCCAACTCCT CCGACCCTGT GGAGATGAGA AGACTTAACT ACCAGACCCC AGGTCCCAGC 
TCTCATCGCT GCCCCAACAC TCCAAGAATG AGGGAGCACC CGCCCATCCC TGTCGTCGAC 
CTGGCTGACC ACATAGAGCG TCTGAAGGCC AACGACGGCC TACGCTTTTC CCAGGAGTAC 
GAGTCGATTG ATCCAGGGCA GCAGTTTACC TGGGAGAACT CCAACATGGA AGTCAACAAG 
CCAAAGAATC GCTATGCAAA CGTCATCGCC TATGACCACT CCAGAGTGGT TCTCACCTCT 
GTTGACGCCG TTCCAGGCAG TGACTACATC AACGCCAACT ACATCGACGG CTACAGGAAG 
CAGAACGCCT ACATCGCCAC GCAGGGGCCA CTTCCAGAGA CACTGAGCGA CTTCTGGAGG 
ATGGTGTGGG AGCAAAGATC CAACACCATC GTCATGATGA CCAGACTGGA GGAGAAGTCG 
CGGGTGAAGT GTGACCAGTA CTGGCCGTCG CGAGGAACGG AGACCTATGG GATGATCCAG 
GTCACCATGC TGGACACCGT GGAGTTGGCC ACTTACAGCG TCCGTACCTT TGCCCTCTAT 
AAGAATGGCT CCAGTGAGAA GAGAGAGGTG AGACAGTTCC AGTTCATGGC CTGGCCAGAC 
CACGGGGTGC CAGAGTACCC CACCCCAATC CTGGCCTTCC TACGGCGGGT AAAGGCCTGC 
AATCCTCCAG ACGCAGGGCC TATGGTGGTC CACTGCAGCG CGGGTGTGGG CAGGACCGGA 
TGCTTCATCG TGATAGATGC CATGCTGGAG CGCATGAAGC ACGAGAAGTC CGTAGACATC 
TACGGCCACG TGACGTGCAT GCGCGCCCAG AGGAACTACA TGGTCCAGAC CGAGGACCAG 
TACATCTTCA TTCACGAAGC CCTGCTGGAG GCCGCCACGT GCGGCAACAC CGAGGTGCCG 
GCGCGCAACC TGTACGCCCA CATCCAGAAA CTCACCCAGC CCCCGCCCGG CGAGACGGTT 
ACTGCCATGG AGCTGGAATT CAAGAGACTG GCCAACTCCA AAGCCCACAC ATCCCGCTTC 
ATCAGCGCCA ACTTGCCCTG CAACAAGTTC AAGAACCGCC TGGTCAACAT CATGCCTTTC 
GAGTCCACCC GCGTCTGCCT GCAGCCCATC CGTGGCGTCG AGGGCTCCGA CTACATCAAT 
GCCAGCTGCA TCGACGGCTA CAGACAGCAG AAGGCCTACC TGGCCACGCA GGGCCCACTG 
GCAGAGACCA CCGAGGACTT CTGGAGGATG CTGTGGGAGC ACAACTCCAC CATCGTCGTC 
ATGCTCACCA AGCTACGAGA GATGGGCCGG GAGAAGTGCC ATCAGTACTG GCCAGCAGAG 
CGTTCAGCCA GGTACCAGTA CTTTGTGGTG GATCCAATGG CTGAGTACAA CATGCCCCAG 
TACATCCTCA GAGAGTTCAA AGTCACAGAT GCCAGGGACG GCCAGTCCAG AACAATCCGC 
CAATTCCAGT TCACTGACTG GCCCGAGCAA GGAGTGCCAA AAACCGGAGA GGGCTTCATT 
GATTTTATCG GACAAGTGCA CAAAACCAAG GAACAGTTCG GACAGGACGG ACCAATCACT 
GTACACTGCA GTGCTGGGGT GGGCAGGACC GGAGTCTTCA TCACCCTCAG CATCGTGCTG 
GAGAGGATGA GGTATGAAGG AGTTGTCGAC CTCTTCCAGA CAGTGAAGAC ACTTCGCACC 
CAGCGGCCTG CCATGGTGCA GACCGAGGAC CAGTACCAGT TGTGCTACAG GGCAGCATTG 
GAGTACCTGG GGAGCTTTGA CCACTATGCA ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104931439 ( XM_019263528.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019119073.1 Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X2, mRNA.

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CloneID	OLa84596
Clone ID Related Accession (Same CDS sequence)	XM_019263528.1 , XM_019263523.1
Accession Version	XM_019263528.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	receptor-type tyrosine-protein phosphatase F isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017607936.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTTGACATC 
AGCAGCAGCA ACGGACGCAT TAAACAGCTT CGTTCAGGTG GTACACCAAT TAGAGGAGCT 
CTGCAAATTG AGAACAGCGA GGAGTCCGAC CAAGGGAAAT ACGAGTGTGT TGCCATGAAC 
AGCGCTGGGA CCCGTTACTC CGCTCCTGCC AATCTCTACG TACGAGTGCG AAGAGTCCCA 
CCCAGATTCT CCATTCCGCC CACCAACCAA GAGGTGATGC CAGGCGGCAG CGTCAACCTG 
ACATGCGTGG CAGTGGGCTC GCCGATGCCC TACGTCAAGT GGACGATGGG CCTGGTGGAG 
CTGACCAAGG AGGAGGAGAT GCCGATGGGA CGCAACGTGC TGGAGGTCAA CAACATCCGC 
GAGTCGGCGA ACTACACCTG CGTGGCCATC TCCTCTCTGG GCATGATAGA AGCCACCGCT 
CAGGTCACCG TCAAAGCTTT GCCAAAGCCT CCCGCCTCCC TCATTGTGAC TGAGACCACA 
GCCACCAGTG TGACTCTCAC CTGGGACTCT GGAAACCCAG AACCTGTCTC CTACTACATG 
ATCCAATACA GAGCCAAGAC CTCGGACAAC GGCTTCCAGG AAGTGGAAGG TGTGGCAACT 
ACCCGTTACA GCATCGGTGG ACTTAGCCCC TACTCTGAGT ATGAGTTTCG TGTCATGGCT 
GTCAACAACA TCGGCCGGGG GCCACCGAGC GACCCCGTGG AGACACGCAC TGGTGAACAG 
GCCCCCTCCT CGCCACCTCT GCACGTCCAA GCGCGCATGC TGAGTGCCAG CACAATGCTG 
GTCCAGTGGG AACCGCCCGA GGAACCCAAC GGGCAAATCA GGGGCTACCG GGTCTACTAC 
AGCTCGGACT TGACGGCTCC GCTCAGCGCC TGGCAGAAGC ACAACACAGA CGACAGCAGC 
CTGACGACCA TCTCGGGCCT GACTCCTGAT ATCACCTACA GCCTCAGGGT GCTGGGCTTC 
ACCTCTGTAG GTGACGGGCC ACCTTCTGAC ATCCTGCAAG TCAAAACCCA ACAGGGAGTC 
CCTGCCCAGC CGTCCAGCTT TGAGGCCGAA GCTGAGCTGG ACAGTCGCAT CATGCTAACA 
TGGCTGTGGC CCGTCCAGGA CCCCATCACT AAGTATGAGC TGCAGTACTG GGAGGCCGGC 
TCAGACAACA AGTTACATGT GAGCTTCAAC CCAGCGGGCT CCTACGCTGT GGACGGTCTA 
AAGCCCGACA CCTTGTACCG TTTCTCTCTG GCAGCCCGTT CTGAGATGGG CTTAGGTGTC 
TACACCCAAC CCATCGAGGC TCGCACCGCC CAGTCCACCC CATCAGCTCC CCCCCAGGAG 
GTACACCTGG TCAGCCTGAG CTCCACCAGC CTCAAGGTGA GTTGGGTGGC ACCGCCCGCC 
GCTAGCCGCC ACGGCGCCAT TGTGCGCTAC ACAGTCAGCT ACCAGGCCAT GGCTGGAGAA 
GACACGGAGC GGCACGAGGT GACGGGCATC GGCGCAGACG CCACTAGCTT TGTGCTGGAG 
GGCCTGGAGA AGTGGACTGA GTATCAGGTG TGGGTGAGGG CACACACTGA CGTGGGCCCC 
GGCCCAGAGA GTGCCCCAGT GAGGATGAGA ACCAAAGAGG ATGTGCCGGG AGCACCCCCC 
AGGAAGCTGG AGGTTGAGGC CATAAACTCC ACAGCAATCC GGGTCACCTG GAAGCCGCCC 
CTGCAGGGGA AGCAGCACGG CCAAATCCGG GGCTATCAAG TCATCTTCTC TCGGCTTGAA 
AACGGCGAAC CACGAGGCCA GCCTAACATC ATGGACGTTG CTTTGCCAGA GGCACAGTGG 
AATATTGAGG AGTCCACCGA GCATGAGGCC ATCATTGCCG GACTGCACTC TGAAACCACC 
TACTCGGTCA CCGTGGCAGC ATACACCACC AAAGGAGACG GAGCGCGCAG CAAAGCCAAA 
GTCATCACCA CGACGGGTGC AGTGCCAGGA AAGCCCACCA TGATGATCAG CACCACTATG 
GGCAACACAG CGCTGATTCA GTGGCAGCCA CCTAAGGAAA TGGTTGGGGA GCTGATGGGC 
TACCAGCTGC AGTACAAGCG CACTGACGAG GAAAACTACA CCTCCCGCGA GCTGAGAAAG 
ATCGATGACC ACTACACCGT CACCGGGCTG CACAAAGGTG CCACCTATGT CTTCCGCCTC 
AGTGCCCGCA ACCGCGCGGG CATCGGCGAA GCATACGTGA AAGAGATCAG CACCCCTGAA 
GACGTGCCGT CGGGCTTCCC GTTCAACCTG CAGGTCACGG GACTGACTCA GTCCAGCACC 
GAGCTGACCT GGGACCCTCC GCTGGTGGCC GAAAGGAACG GCAAGATCGT GCACTATGTG 
GTCGTTTACA GGGACATCAA CAGTCAGCAG AACAGCACCA ACCGGACGAG CGAAACGCAC 
ATGACCATCC AGGGCCTTAA TCCCGACACC ACCTACGATA TCCGGGTGCA GGCGTTCACC 
AGTAAGGGCG GAGGACCCCT CAGCCCTAGC ATCCAGAGCA GGACCATGTC GAACGATTTT 
CCCACCCTCA ACTTTGGTGT CAAAGCCGTC ATGAAAACAT CTGTCCTCCT CACCTGGGAT 
TTGCCACCGA ACTACAAATC CCAAGTGCCT TTCAAGATCC TGTACAACCA GCAAAGCGTG 
GAAGTGCAGG GATCCCTGAA GAGGAGGCTG ATCACGCAGC TGCAGCCAGA CACGGACTAC 
TCTTTTGTGC TGACAAGCAG GGGCAACATT GCTGGTGGTC TACAGCAGCA GGTGTCCATC 
CGCACCGCTC CCGACCTGAT CAACGCCAAG CCCAGCACAC ATCAGCCTCA GGGTAGCAAG 
ATGACAATCA ACCTGCCCAA GGTCCAGACC ACCACGCGCG TCAGGTGGTA CTACATCGTG 
GTGGTGCCGG TGTCCCAGTC AACGCAGGGG TGGAAGAATC CAGACGAGAT GGACCTGGAC 
GAGCTGTTCA AGTCAAGTGA AGGCCCCGTC CTGAGGCGCA GACGTCACCT GGATTCCTCC 
AAACCTTACA TCGCTGCCAA GTTGGCCTCG TTGCCGACCA CCTTCACCCT GGGTGATGAG 
AAGAGATACA ACGGCTTCTA CAACAAGCCC CTGAGCAGTC CGCAGGAGTA TCTCTGCTTT 
GTCCTGGCAG TACTCCGATC CGAAGGGGCA GACAGCACTA CTAACTATAT GACATTTTCA 
GCCAGTCCCT ACTCAGACCC GGTCCACGTG CGAAACAGCA TGGCTCAGGG GATGGAGCAG 
CCTGAAATGT TGTGGGTTAT GGGCCCTGTT TTGGCCGTGG TCCTGATTAT CATCATCGTC 
ATCGCCATCC TGCTCTTCAA GAACAAACAG GAAAGGAAGC GAGCGTCGCC CCTGCCCAAA 
GATGAGCACT CGGGCGGGGT GAAGGACTCC CTGCTGGCCA ACTCCTCCGA CCCTGTGGAG 
ATGAGAAGAC TTAACTACCA GACCCCAGGT CCCAGCTCTC ATCGCTGCCC CAACACTCCA 
AGAATGAGGG AGCACCCGCC CATCCCTGTC GTCGACCTGG CTGACCACAT AGAGCGTCTG 
AAGGCCAACG ACGGCCTACG CTTTTCCCAG GAGTACGAGT CGATTGATCC AGGGCAGCAG 
TTTACCTGGG AGAACTCCAA CATGGAAGTC AACAAGCCAA AGAATCGCTA TGCAAACGTC 
ATTGCCTATG ACCACTCCAG AGTGGTTCTC ACCTCTGTTG ACGCCGTTCC AGGCAGTGAC 
TACATCAACG CCAACTACAT CGACGGCTAC AGGAAGCAGA ACGCCTACAT CGCCACGCAG 
GGGCCACTTC CAGAGACACT GAGCGACTTC TGGAGGATGG TGTGGGAGCA AAGATCCAAC 
ACCATCGTCA TGATGACCAG ACTGGAGGAG AAGTCACGGG TGAAGTGTGA CCAGTACTGG 
CCGTCGCGAG GAACGGAGAC CTATGGGATG ATCCAGGTCA CCATGCTGGA CACCGTGGAG 
TTGGCCACTT ACAGCGTCCG TACCTTTGCC CTCTATAAGA ATGGCTCCAG TGAGAAGAGA 
GAGGTGAGAC AGTTCCAGTT CATGGCCTGG CCAGACCACG GGGTGCCAGA GTACCCCACC 
CCAATCCTGG CCTTCCTACG GCGGGTAAAG GCCTGCAATC CTCCAGACGC AGGGCCTATG 
GTGGTCCACT GCAGCGCGGG TGTGGGCAGG ACCGGATGCT TCATCGTGAT AGATGCCATG 
CTGGAGCGCA TGAAGCACGA GAAGTCCGTA GACATCTACG GCCACGTGAC GTGCATGCGC 
GCCCAGAGGA ACTACATGGT CCAGACCGAG GACCAGTACA TCTTCATTCA CGAAGCCCTG 
CTGGAGGCCG CCACGTGCGG CAACACCGAG GTGCCGGCGC GCAACCTGTA CGCCCACATC 
CAGAAACTCA CCCAGCCCCC GCCCGGCGAG ACGGTTACTG CCATGGAGCT GGAATTCAAG 
AGACTGGCCA ACTCCAAAGC CCACACATCC CGCTTCATCA GCGCCAACTT GCCCTGCAAC 
AAGTTCAAGA ACCGCCTGGT CAACATCATG CCTTTCGAGT CCACCCGTGT CTGCCTGCAG 
CCCATCCGTG GCGTCGAGGG CTCCGACTAC ATCAATGCCA GCTGCATCGA CGGCTACAGA 
CAGCAGAAGG CCTACCTGGC CACGCAGGGC CCACTGGCAG AGACCACCGA GGACTTCTGG 
AGGATGCTGT GGGAGCACAA CTCCACCATC GTCGTCATGC TCACCAAGCT ACGAGAGATG 
GGCCGGGAGA AGTGCCATCA GTACTGGCCA GCAGAGCGTT CAGCCAGGTA CCAGTACTTT 
GTGGTGGATC CAATGGCTGA GTACAACATG CCCCAGTACA TCCTCAGAGA GTTCAAAGTC 
ACAGATGCCA GGGACGGCCA GTCCAGAACA ATCCGCCAAT TCCAGTTCAC TGACTGGCCC 
GAGCAAGGAG TGCCAAAAAC CGGAGAGGGC TTCATTGATT TTATCGGACA AGTGCACAAA 
ACCAAGGAAC AGTTCGGACA GGACGGACCA ATCACTGTAC ACTGCAGTGC TGGGGTGGGC 
AGGACCGGAG TCTTCATCAC CCTCAGCATC GTGCTGGAGA GGATGAGGTA TGAAGGAGTT 
GTCGACCTCT TCCAGACAGT GAAGACACTT CGCACCCAGC GGCCTGCCAT GGTGCAGACC 
GAGGACCAGT ACCAGTTGTG CTACAGGGCA GCATTGGAGT ACCTGGGGAG CTTTGACCAC 
TATGCAACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019119073.1
CDS	726..6497
Translation	MVPNIHLAVPFLPRGALLFLILLSCVVFSSLADSMPSFVKSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVEKTRTATMLCAASGNPDPEISWFKDMLPVDISSSNGRIKQLRSGGTPIRGALQIENSEESDQGKYECVAMNSAGTRYSAPANLYVRVRRVPPRFSIPPTNQEVMPGGSVNLTCVAVGSPMPYVKWTMGLVELTKEEEMPMGRNVLEVNNIRESANYTCVAISSLGMIEATAQVTVKALPKPPASLIVTETTATSVTLTWDSGNPEPVSYYMIQYRAKTSDNGFQEVEGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSDPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRVYYSSDLTAPLSAWQKHNTDDSSLTTISGLTPDITYSLRVLGFTSVGDGPPSDILQVKTQQGVPAQPSSFEAEAELDSRIMLTWLWPVQDPITKYELQYWEAGSDNKLHVSFNPAGSYAVDGLKPDTLYRFSLAARSEMGLGVYTQPIEARTAQSTPSAPPQEVHLVSLSSTSLKVSWVAPPAASRHGAIVRYTVSYQAMAGEDTERHEVTGIGADATSFVLEGLEKWTEYQVWVRAHTDVGPGPESAPVRMRTKEDVPGAPPRKLEVEAINSTAIRVTWKPPLQGKQHGQIRGYQVIFSRLENGEPRGQPNIMDVALPEAQWNIEESTEHEAIIAGLHSETTYSVTVAAYTTKGDGARSKAKVITTTGAVPGKPTMMISTTMGNTALIQWQPPKEMVGELMGYQLQYKRTDEENYTSRELRKIDDHYTVTGLHKGATYVFRLSARNRAGIGEAYVKEISTPEDVPSGFPFNLQVTGLTQSSTELTWDPPLVAERNGKIVHYVVVYRDINSQQNSTNRTSETHMTIQGLNPDTTYDIRVQAFTSKGGGPLSPSIQSRTMSNDFPTLNFGVKAVMKTSVLLTWDLPPNYKSQVPFKILYNQQSVEVQGSLKRRLITQLQPDTDYSFVLTSRGNIAGGLQQQVSIRTAPDLINAKPSTHQPQGSKMTINLPKVQTTTRVRWYYIVVVPVSQSTQGWKNPDEMDLDELFKSSEGPVLRRRRHLDSSKPYIAAKLASLPTTFTLGDEKRYNGFYNKPLSSPQEYLCFVLAVLRSEGADSTTNYMTFSASPYSDPVHVRNSMAQGMEQPEMLWVMGPVLAVVLIIIIVIAILLFKNKQERKRASPLPKDEHSGGVKDSLLANSSDPVEMRRLNYQTPGPSSHRCPNTPRMREHPPIPVVDLADHIERLKANDGLRFSQEYESIDPGQQFTWENSNMEVNKPKNRYANVIAYDHSRVVLTSVDAVPGSDYINANYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRSNTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVTMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRAQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPPPGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYLATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_019263528.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019263528.1

ORF Insert Sequence:

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTTGACATC 
AGCAGCAGCA ACGGACGCAT TAAACAGCTT CGTTCAGGTG GTACACCAAT TAGAGGAGCT 
CTGCAAATTG AGAACAGCGA GGAGTCCGAC CAAGGGAAAT ACGAGTGTGT TGCCATGAAC 
AGCGCTGGGA CCCGTTACTC CGCTCCTGCC AATCTCTACG TACGAGTGCG AAGAGTCCCA 
CCCAGATTCT CCATTCCGCC CACCAACCAA GAGGTGATGC CAGGCGGCAG CGTCAACCTG 
ACATGCGTGG CAGTGGGCTC GCCGATGCCC TACGTCAAGT GGACGATGGG CCTGGTGGAG 
CTGACCAAGG AGGAGGAGAT GCCGATGGGA CGCAACGTGC TGGAGGTCAA CAACATCCGC 
GAGTCGGCGA ACTACACCTG CGTGGCCATC TCCTCTCTGG GCATGATAGA AGCCACCGCT 
CAGGTCACCG TCAAAGCTTT GCCAAAGCCT CCCGCCTCCC TCATTGTGAC TGAGACCACA 
GCCACCAGTG TGACTCTCAC CTGGGACTCT GGAAACCCAG AACCTGTCTC CTACTACATG 
ATCCAATACA GAGCCAAGAC CTCGGACAAC GGCTTCCAGG AAGTGGAAGG TGTGGCAACT 
ACCCGTTACA GCATCGGTGG ACTTAGCCCC TACTCTGAGT ATGAGTTTCG TGTCATGGCT 
GTCAACAACA TCGGCCGGGG GCCACCGAGC GACCCCGTGG AGACACGCAC TGGTGAACAG 
GCCCCCTCCT CGCCACCTCT GCACGTCCAA GCGCGCATGC TGAGTGCCAG CACAATGCTG 
GTCCAGTGGG AACCGCCCGA GGAACCCAAC GGGCAAATCA GGGGCTACCG GGTCTACTAC 
AGCTCGGACT TGACGGCTCC GCTCAGCGCC TGGCAGAAGC ACAACACAGA CGACAGCAGC 
CTGACGACCA TCTCGGGCCT GACTCCTGAT ATCACCTACA GCCTCAGGGT GCTGGGCTTC 
ACCTCTGTAG GTGACGGGCC ACCTTCTGAC ATCCTGCAAG TCAAAACCCA ACAGGGAGTC 
CCTGCCCAGC CGTCCAGCTT TGAGGCCGAA GCTGAGCTGG ACAGTCGCAT CATGCTAACA 
TGGCTGTGGC CCGTCCAGGA CCCCATCACT AAGTATGAGC TGCAGTACTG GGAGGCCGGC 
TCAGACAACA AGTTACATGT GAGCTTCAAC CCAGCGGGCT CCTACGCTGT GGACGGTCTA 
AAGCCCGACA CCTTGTACCG TTTCTCTCTG GCAGCCCGTT CTGAGATGGG CTTAGGTGTC 
TACACCCAAC CCATCGAGGC TCGCACCGCC CAGTCCACCC CATCAGCTCC CCCCCAGGAG 
GTACACCTGG TCAGCCTGAG CTCCACCAGC CTCAAGGTGA GTTGGGTGGC ACCGCCCGCC 
GCTAGCCGCC ACGGCGCCAT TGTGCGCTAC ACAGTCAGCT ACCAGGCCAT GGCTGGAGAA 
GACACGGAGC GGCACGAGGT GACGGGCATC GGCGCAGACG CCACTAGCTT TGTGCTGGAG 
GGCCTGGAGA AGTGGACTGA GTATCAGGTG TGGGTGAGGG CACACACTGA CGTGGGCCCC 
GGCCCAGAGA GTGCCCCAGT GAGGATGAGA ACCAAAGAGG ATGTGCCGGG AGCACCCCCC 
AGGAAGCTGG AGGTTGAGGC CATAAACTCC ACAGCAATCC GGGTCACCTG GAAGCCGCCC 
CTGCAGGGGA AGCAGCACGG CCAAATCCGG GGCTATCAAG TCATCTTCTC TCGGCTTGAA 
AACGGCGAAC CACGAGGCCA GCCTAACATC ATGGACGTTG CTTTGCCAGA GGCACAGTGG 
AATATTGAGG AGTCCACCGA GCATGAGGCC ATCATTGCCG GACTGCACTC TGAAACCACC 
TACTCGGTCA CCGTGGCAGC ATACACCACC AAAGGAGACG GAGCGCGCAG CAAAGCCAAA 
GTCATCACCA CGACGGGTGC AGTGCCAGGA AAGCCCACCA TGATGATCAG CACCACTATG 
GGCAACACAG CGCTGATTCA GTGGCAGCCA CCTAAGGAAA TGGTTGGGGA GCTGATGGGC 
TACCAGCTGC AGTACAAGCG CACTGACGAG GAAAACTACA CCTCCCGCGA GCTGAGAAAG 
ATCGATGACC ACTACACCGT CACCGGGCTG CACAAAGGTG CCACCTATGT CTTCCGCCTC 
AGTGCCCGCA ACCGCGCGGG CATCGGCGAA GCATACGTGA AAGAGATCAG CACCCCTGAA 
GACGTGCCGT CGGGCTTCCC GTTCAACCTG CAGGTCACGG GACTGACTCA GTCCAGCACC 
GAGCTGACCT GGGACCCTCC GCTGGTGGCC GAAAGGAACG GCAAGATCGT GCACTATGTG 
GTCGTTTACA GGGACATCAA CAGTCAGCAG AACAGCACCA ACCGGACGAG CGAAACGCAC 
ATGACCATCC AGGGCCTTAA TCCCGACACC ACCTACGATA TCCGGGTGCA GGCGTTCACC 
AGTAAGGGCG GAGGACCCCT CAGCCCTAGC ATCCAGAGCA GGACCATGTC GAACGATTTT 
CCCACCCTCA ACTTTGGTGT CAAAGCCGTC ATGAAAACAT CTGTCCTCCT CACCTGGGAT 
TTGCCACCGA ACTACAAATC CCAAGTGCCT TTCAAGATCC TGTACAACCA GCAAAGCGTG 
GAAGTGCAGG GATCCCTGAA GAGGAGGCTG ATCACGCAGC TGCAGCCAGA CACGGACTAC 
TCTTTTGTGC TGACAAGCAG GGGCAACATT GCTGGTGGTC TACAGCAGCA GGTGTCCATC 
CGCACCGCTC CCGACCTGAT CAACGCCAAG CCCAGCACAC ATCAGCCTCA GGGTAGCAAG 
ATGACAATCA ACCTGCCCAA GGTCCAGACC ACCACGCGCG TCAGGTGGTA CTACATCGTG 
GTGGTGCCGG TGTCCCAGTC AACGCAGGGG TGGAAGAATC CAGACGAGAT GGACCTGGAC 
GAGCTGTTCA AGTCAAGTGA AGGCCCCGTC CTGAGGCGCA GACGTCACCT GGATTCCTCC 
AAACCTTACA TCGCTGCCAA GTTGGCCTCG TTGCCGACCA CCTTCACCCT GGGTGATGAG 
AAGAGATACA ACGGCTTCTA CAACAAGCCC CTGAGCAGTC CGCAGGAGTA TCTCTGCTTT 
GTCCTGGCAG TACTCCGATC CGAAGGGGCA GACAGCACTA CTAACTATAT GACATTTTCA 
GCCAGTCCCT ACTCAGACCC GGTCCACGTG CGAAACAGCA TGGCTCAGGG GATGGAGCAG 
CCTGAAATGT TGTGGGTTAT GGGCCCTGTT TTGGCCGTGG TCCTGATTAT CATCATCGTC 
ATCGCCATCC TGCTCTTCAA GAACAAACAG GAAAGGAAGC GAGCGTCGCC CCTGCCCAAA 
GATGAGCACT CGGGCGGGGT GAAGGACTCC CTGCTGGCCA ACTCCTCCGA CCCTGTGGAG 
ATGAGAAGAC TTAACTACCA GACCCCAGGT CCCAGCTCTC ATCGCTGCCC CAACACTCCA 
AGAATGAGGG AGCACCCGCC CATCCCTGTC GTCGACCTGG CTGACCACAT AGAGCGTCTG 
AAGGCCAACG ACGGCCTACG CTTTTCCCAG GAGTACGAGT CGATTGATCC AGGGCAGCAG 
TTTACCTGGG AGAACTCCAA CATGGAAGTC AACAAGCCAA AGAATCGCTA TGCAAACGTC 
ATTGCCTATG ACCACTCCAG AGTGGTTCTC ACCTCTGTTG ACGCCGTTCC AGGCAGTGAC 
TACATCAACG CCAACTACAT CGACGGCTAC AGGAAGCAGA ACGCCTACAT CGCCACGCAG 
GGGCCACTTC CAGAGACACT GAGCGACTTC TGGAGGATGG TGTGGGAGCA AAGATCCAAC 
ACCATCGTCA TGATGACCAG ACTGGAGGAG AAGTCACGGG TGAAGTGTGA CCAGTACTGG 
CCGTCGCGAG GAACGGAGAC CTATGGGATG ATCCAGGTCA CCATGCTGGA CACCGTGGAG 
TTGGCCACTT ACAGCGTCCG TACCTTTGCC CTCTATAAGA ATGGCTCCAG TGAGAAGAGA 
GAGGTGAGAC AGTTCCAGTT CATGGCCTGG CCAGACCACG GGGTGCCAGA GTACCCCACC 
CCAATCCTGG CCTTCCTACG GCGGGTAAAG GCCTGCAATC CTCCAGACGC AGGGCCTATG 
GTGGTCCACT GCAGCGCGGG TGTGGGCAGG ACCGGATGCT TCATCGTGAT AGATGCCATG 
CTGGAGCGCA TGAAGCACGA GAAGTCCGTA GACATCTACG GCCACGTGAC GTGCATGCGC 
GCCCAGAGGA ACTACATGGT CCAGACCGAG GACCAGTACA TCTTCATTCA CGAAGCCCTG 
CTGGAGGCCG CCACGTGCGG CAACACCGAG GTGCCGGCGC GCAACCTGTA CGCCCACATC 
CAGAAACTCA CCCAGCCCCC GCCCGGCGAG ACGGTTACTG CCATGGAGCT GGAATTCAAG 
AGACTGGCCA ACTCCAAAGC CCACACATCC CGCTTCATCA GCGCCAACTT GCCCTGCAAC 
AAGTTCAAGA ACCGCCTGGT CAACATCATG CCTTTCGAGT CCACCCGTGT CTGCCTGCAG 
CCCATCCGTG GCGTCGAGGG CTCCGACTAC ATCAATGCCA GCTGCATCGA CGGCTACAGA 
CAGCAGAAGG CCTACCTGGC CACGCAGGGC CCACTGGCAG AGACCACCGA GGACTTCTGG 
AGGATGCTGT GGGAGCACAA CTCCACCATC GTCGTCATGC TCACCAAGCT ACGAGAGATG 
GGCCGGGAGA AGTGCCATCA GTACTGGCCA GCAGAGCGTT CAGCCAGGTA CCAGTACTTT 
GTGGTGGATC CAATGGCTGA GTACAACATG CCCCAGTACA TCCTCAGAGA GTTCAAAGTC 
ACAGATGCCA GGGACGGCCA GTCCAGAACA ATCCGCCAAT TCCAGTTCAC TGACTGGCCC 
GAGCAAGGAG TGCCAAAAAC CGGAGAGGGC TTCATTGATT TTATCGGACA AGTGCACAAA 
ACCAAGGAAC AGTTCGGACA GGACGGACCA ATCACTGTAC ACTGCAGTGC TGGGGTGGGC 
AGGACCGGAG TCTTCATCAC CCTCAGCATC GTGCTGGAGA GGATGAGGTA TGAAGGAGTT 
GTCGACCTCT TCCAGACAGT GAAGACACTT CGCACCCAGC GGCCTGCCAT GGTGCAGACC 
GAGGACCAGT ACCAGTTGTG CTACAGGGCA GCATTGGAGT ACCTGGGGAG CTTTGACCAC 
TATGCAACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104931439 ( XM_019263523.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019119068.1 Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X1, mRNA.

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CloneID	OLa84596
Clone ID Related Accession (Same CDS sequence)	XM_019263528.1 , XM_019263523.1
Accession Version	XM_019263523.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	receptor-type tyrosine-protein phosphatase F isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017607936.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTTGACATC 
AGCAGCAGCA ACGGACGCAT TAAACAGCTT CGTTCAGGTG GTACACCAAT TAGAGGAGCT 
CTGCAAATTG AGAACAGCGA GGAGTCCGAC CAAGGGAAAT ACGAGTGTGT TGCCATGAAC 
AGCGCTGGGA CCCGTTACTC CGCTCCTGCC AATCTCTACG TACGAGTGCG AAGAGTCCCA 
CCCAGATTCT CCATTCCGCC CACCAACCAA GAGGTGATGC CAGGCGGCAG CGTCAACCTG 
ACATGCGTGG CAGTGGGCTC GCCGATGCCC TACGTCAAGT GGACGATGGG CCTGGTGGAG 
CTGACCAAGG AGGAGGAGAT GCCGATGGGA CGCAACGTGC TGGAGGTCAA CAACATCCGC 
GAGTCGGCGA ACTACACCTG CGTGGCCATC TCCTCTCTGG GCATGATAGA AGCCACCGCT 
CAGGTCACCG TCAAAGCTTT GCCAAAGCCT CCCGCCTCCC TCATTGTGAC TGAGACCACA 
GCCACCAGTG TGACTCTCAC CTGGGACTCT GGAAACCCAG AACCTGTCTC CTACTACATG 
ATCCAATACA GAGCCAAGAC CTCGGACAAC GGCTTCCAGG AAGTGGAAGG TGTGGCAACT 
ACCCGTTACA GCATCGGTGG ACTTAGCCCC TACTCTGAGT ATGAGTTTCG TGTCATGGCT 
GTCAACAACA TCGGCCGGGG GCCACCGAGC GACCCCGTGG AGACACGCAC TGGTGAACAG 
GCCCCCTCCT CGCCACCTCT GCACGTCCAA GCGCGCATGC TGAGTGCCAG CACAATGCTG 
GTCCAGTGGG AACCGCCCGA GGAACCCAAC GGGCAAATCA GGGGCTACCG GGTCTACTAC 
AGCTCGGACT TGACGGCTCC GCTCAGCGCC TGGCAGAAGC ACAACACAGA CGACAGCAGC 
CTGACGACCA TCTCGGGCCT GACTCCTGAT ATCACCTACA GCCTCAGGGT GCTGGGCTTC 
ACCTCTGTAG GTGACGGGCC ACCTTCTGAC ATCCTGCAAG TCAAAACCCA ACAGGGAGTC 
CCTGCCCAGC CGTCCAGCTT TGAGGCCGAA GCTGAGCTGG ACAGTCGCAT CATGCTAACA 
TGGCTGTGGC CCGTCCAGGA CCCCATCACT AAGTATGAGC TGCAGTACTG GGAGGCCGGC 
TCAGACAACA AGTTACATGT GAGCTTCAAC CCAGCGGGCT CCTACGCTGT GGACGGTCTA 
AAGCCCGACA CCTTGTACCG TTTCTCTCTG GCAGCCCGTT CTGAGATGGG CTTAGGTGTC 
TACACCCAAC CCATCGAGGC TCGCACCGCC CAGTCCACCC CATCAGCTCC CCCCCAGGAG 
GTACACCTGG TCAGCCTGAG CTCCACCAGC CTCAAGGTGA GTTGGGTGGC ACCGCCCGCC 
GCTAGCCGCC ACGGCGCCAT TGTGCGCTAC ACAGTCAGCT ACCAGGCCAT GGCTGGAGAA 
GACACGGAGC GGCACGAGGT GACGGGCATC GGCGCAGACG CCACTAGCTT TGTGCTGGAG 
GGCCTGGAGA AGTGGACTGA GTATCAGGTG TGGGTGAGGG CACACACTGA CGTGGGCCCC 
GGCCCAGAGA GTGCCCCAGT GAGGATGAGA ACCAAAGAGG ATGTGCCGGG AGCACCCCCC 
AGGAAGCTGG AGGTTGAGGC CATAAACTCC ACAGCAATCC GGGTCACCTG GAAGCCGCCC 
CTGCAGGGGA AGCAGCACGG CCAAATCCGG GGCTATCAAG TCATCTTCTC TCGGCTTGAA 
AACGGCGAAC CACGAGGCCA GCCTAACATC ATGGACGTTG CTTTGCCAGA GGCACAGTGG 
AATATTGAGG AGTCCACCGA GCATGAGGCC ATCATTGCCG GACTGCACTC TGAAACCACC 
TACTCGGTCA CCGTGGCAGC ATACACCACC AAAGGAGACG GAGCGCGCAG CAAAGCCAAA 
GTCATCACCA CGACGGGTGC AGTGCCAGGA AAGCCCACCA TGATGATCAG CACCACTATG 
GGCAACACAG CGCTGATTCA GTGGCAGCCA CCTAAGGAAA TGGTTGGGGA GCTGATGGGC 
TACCAGCTGC AGTACAAGCG CACTGACGAG GAAAACTACA CCTCCCGCGA GCTGAGAAAG 
ATCGATGACC ACTACACCGT CACCGGGCTG CACAAAGGTG CCACCTATGT CTTCCGCCTC 
AGTGCCCGCA ACCGCGCGGG CATCGGCGAA GCATACGTGA AAGAGATCAG CACCCCTGAA 
GACGTGCCGT CGGGCTTCCC GTTCAACCTG CAGGTCACGG GACTGACTCA GTCCAGCACC 
GAGCTGACCT GGGACCCTCC GCTGGTGGCC GAAAGGAACG GCAAGATCGT GCACTATGTG 
GTCGTTTACA GGGACATCAA CAGTCAGCAG AACAGCACCA ACCGGACGAG CGAAACGCAC 
ATGACCATCC AGGGCCTTAA TCCCGACACC ACCTACGATA TCCGGGTGCA GGCGTTCACC 
AGTAAGGGCG GAGGACCCCT CAGCCCTAGC ATCCAGAGCA GGACCATGTC GAACGATTTT 
CCCACCCTCA ACTTTGGTGT CAAAGCCGTC ATGAAAACAT CTGTCCTCCT CACCTGGGAT 
TTGCCACCGA ACTACAAATC CCAAGTGCCT TTCAAGATCC TGTACAACCA GCAAAGCGTG 
GAAGTGCAGG GATCCCTGAA GAGGAGGCTG ATCACGCAGC TGCAGCCAGA CACGGACTAC 
TCTTTTGTGC TGACAAGCAG GGGCAACATT GCTGGTGGTC TACAGCAGCA GGTGTCCATC 
CGCACCGCTC CCGACCTGAT CAACGCCAAG CCCAGCACAC ATCAGCCTCA GGGTAGCAAG 
ATGACAATCA ACCTGCCCAA GGTCCAGACC ACCACGCGCG TCAGGTGGTA CTACATCGTG 
GTGGTGCCGG TGTCCCAGTC AACGCAGGGG TGGAAGAATC CAGACGAGAT GGACCTGGAC 
GAGCTGTTCA AGTCAAGTGA AGGCCCCGTC CTGAGGCGCA GACGTCACCT GGATTCCTCC 
AAACCTTACA TCGCTGCCAA GTTGGCCTCG TTGCCGACCA CCTTCACCCT GGGTGATGAG 
AAGAGATACA ACGGCTTCTA CAACAAGCCC CTGAGCAGTC CGCAGGAGTA TCTCTGCTTT 
GTCCTGGCAG TACTCCGATC CGAAGGGGCA GACAGCACTA CTAACTATAT GACATTTTCA 
GCCAGTCCCT ACTCAGACCC GGTCCACGTG CGAAACAGCA TGGCTCAGGG GATGGAGCAG 
CCTGAAATGT TGTGGGTTAT GGGCCCTGTT TTGGCCGTGG TCCTGATTAT CATCATCGTC 
ATCGCCATCC TGCTCTTCAA GAACAAACAG GAAAGGAAGC GAGCGTCGCC CCTGCCCAAA 
GATGAGCACT CGGGCGGGGT GAAGGACTCC CTGCTGGCCA ACTCCTCCGA CCCTGTGGAG 
ATGAGAAGAC TTAACTACCA GACCCCAGGT CCCAGCTCTC ATCGCTGCCC CAACACTCCA 
AGAATGAGGG AGCACCCGCC CATCCCTGTC GTCGACCTGG CTGACCACAT AGAGCGTCTG 
AAGGCCAACG ACGGCCTACG CTTTTCCCAG GAGTACGAGT CGATTGATCC AGGGCAGCAG 
TTTACCTGGG AGAACTCCAA CATGGAAGTC AACAAGCCAA AGAATCGCTA TGCAAACGTC 
ATTGCCTATG ACCACTCCAG AGTGGTTCTC ACCTCTGTTG ACGCCGTTCC AGGCAGTGAC 
TACATCAACG CCAACTACAT CGACGGCTAC AGGAAGCAGA ACGCCTACAT CGCCACGCAG 
GGGCCACTTC CAGAGACACT GAGCGACTTC TGGAGGATGG TGTGGGAGCA AAGATCCAAC 
ACCATCGTCA TGATGACCAG ACTGGAGGAG AAGTCACGGG TGAAGTGTGA CCAGTACTGG 
CCGTCGCGAG GAACGGAGAC CTATGGGATG ATCCAGGTCA CCATGCTGGA CACCGTGGAG 
TTGGCCACTT ACAGCGTCCG TACCTTTGCC CTCTATAAGA ATGGCTCCAG TGAGAAGAGA 
GAGGTGAGAC AGTTCCAGTT CATGGCCTGG CCAGACCACG GGGTGCCAGA GTACCCCACC 
CCAATCCTGG CCTTCCTACG GCGGGTAAAG GCCTGCAATC CTCCAGACGC AGGGCCTATG 
GTGGTCCACT GCAGCGCGGG TGTGGGCAGG ACCGGATGCT TCATCGTGAT AGATGCCATG 
CTGGAGCGCA TGAAGCACGA GAAGTCCGTA GACATCTACG GCCACGTGAC GTGCATGCGC 
GCCCAGAGGA ACTACATGGT CCAGACCGAG GACCAGTACA TCTTCATTCA CGAAGCCCTG 
CTGGAGGCCG CCACGTGCGG CAACACCGAG GTGCCGGCGC GCAACCTGTA CGCCCACATC 
CAGAAACTCA CCCAGCCCCC GCCCGGCGAG ACGGTTACTG CCATGGAGCT GGAATTCAAG 
AGACTGGCCA ACTCCAAAGC CCACACATCC CGCTTCATCA GCGCCAACTT GCCCTGCAAC 
AAGTTCAAGA ACCGCCTGGT CAACATCATG CCTTTCGAGT CCACCCGTGT CTGCCTGCAG 
CCCATCCGTG GCGTCGAGGG CTCCGACTAC ATCAATGCCA GCTGCATCGA CGGCTACAGA 
CAGCAGAAGG CCTACCTGGC CACGCAGGGC CCACTGGCAG AGACCACCGA GGACTTCTGG 
AGGATGCTGT GGGAGCACAA CTCCACCATC GTCGTCATGC TCACCAAGCT ACGAGAGATG 
GGCCGGGAGA AGTGCCATCA GTACTGGCCA GCAGAGCGTT CAGCCAGGTA CCAGTACTTT 
GTGGTGGATC CAATGGCTGA GTACAACATG CCCCAGTACA TCCTCAGAGA GTTCAAAGTC 
ACAGATGCCA GGGACGGCCA GTCCAGAACA ATCCGCCAAT TCCAGTTCAC TGACTGGCCC 
GAGCAAGGAG TGCCAAAAAC CGGAGAGGGC TTCATTGATT TTATCGGACA AGTGCACAAA 
ACCAAGGAAC AGTTCGGACA GGACGGACCA ATCACTGTAC ACTGCAGTGC TGGGGTGGGC 
AGGACCGGAG TCTTCATCAC CCTCAGCATC GTGCTGGAGA GGATGAGGTA TGAAGGAGTT 
GTCGACCTCT TCCAGACAGT GAAGACACTT CGCACCCAGC GGCCTGCCAT GGTGCAGACC 
GAGGACCAGT ACCAGTTGTG CTACAGGGCA GCATTGGAGT ACCTGGGGAG CTTTGACCAC 
TATGCAACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019119068.1
CDS	523..6294
Translation	MVPNIHLAVPFLPRGALLFLILLSCVVFSSLADSMPSFVKSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVEKTRTATMLCAASGNPDPEISWFKDMLPVDISSSNGRIKQLRSGGTPIRGALQIENSEESDQGKYECVAMNSAGTRYSAPANLYVRVRRVPPRFSIPPTNQEVMPGGSVNLTCVAVGSPMPYVKWTMGLVELTKEEEMPMGRNVLEVNNIRESANYTCVAISSLGMIEATAQVTVKALPKPPASLIVTETTATSVTLTWDSGNPEPVSYYMIQYRAKTSDNGFQEVEGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSDPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRVYYSSDLTAPLSAWQKHNTDDSSLTTISGLTPDITYSLRVLGFTSVGDGPPSDILQVKTQQGVPAQPSSFEAEAELDSRIMLTWLWPVQDPITKYELQYWEAGSDNKLHVSFNPAGSYAVDGLKPDTLYRFSLAARSEMGLGVYTQPIEARTAQSTPSAPPQEVHLVSLSSTSLKVSWVAPPAASRHGAIVRYTVSYQAMAGEDTERHEVTGIGADATSFVLEGLEKWTEYQVWVRAHTDVGPGPESAPVRMRTKEDVPGAPPRKLEVEAINSTAIRVTWKPPLQGKQHGQIRGYQVIFSRLENGEPRGQPNIMDVALPEAQWNIEESTEHEAIIAGLHSETTYSVTVAAYTTKGDGARSKAKVITTTGAVPGKPTMMISTTMGNTALIQWQPPKEMVGELMGYQLQYKRTDEENYTSRELRKIDDHYTVTGLHKGATYVFRLSARNRAGIGEAYVKEISTPEDVPSGFPFNLQVTGLTQSSTELTWDPPLVAERNGKIVHYVVVYRDINSQQNSTNRTSETHMTIQGLNPDTTYDIRVQAFTSKGGGPLSPSIQSRTMSNDFPTLNFGVKAVMKTSVLLTWDLPPNYKSQVPFKILYNQQSVEVQGSLKRRLITQLQPDTDYSFVLTSRGNIAGGLQQQVSIRTAPDLINAKPSTHQPQGSKMTINLPKVQTTTRVRWYYIVVVPVSQSTQGWKNPDEMDLDELFKSSEGPVLRRRRHLDSSKPYIAAKLASLPTTFTLGDEKRYNGFYNKPLSSPQEYLCFVLAVLRSEGADSTTNYMTFSASPYSDPVHVRNSMAQGMEQPEMLWVMGPVLAVVLIIIIVIAILLFKNKQERKRASPLPKDEHSGGVKDSLLANSSDPVEMRRLNYQTPGPSSHRCPNTPRMREHPPIPVVDLADHIERLKANDGLRFSQEYESIDPGQQFTWENSNMEVNKPKNRYANVIAYDHSRVVLTSVDAVPGSDYINANYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRSNTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVTMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRAQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPPPGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYLATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_019263523.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019263523.1

ORF Insert Sequence:

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTTGACATC 
AGCAGCAGCA ACGGACGCAT TAAACAGCTT CGTTCAGGTG GTACACCAAT TAGAGGAGCT 
CTGCAAATTG AGAACAGCGA GGAGTCCGAC CAAGGGAAAT ACGAGTGTGT TGCCATGAAC 
AGCGCTGGGA CCCGTTACTC CGCTCCTGCC AATCTCTACG TACGAGTGCG AAGAGTCCCA 
CCCAGATTCT CCATTCCGCC CACCAACCAA GAGGTGATGC CAGGCGGCAG CGTCAACCTG 
ACATGCGTGG CAGTGGGCTC GCCGATGCCC TACGTCAAGT GGACGATGGG CCTGGTGGAG 
CTGACCAAGG AGGAGGAGAT GCCGATGGGA CGCAACGTGC TGGAGGTCAA CAACATCCGC 
GAGTCGGCGA ACTACACCTG CGTGGCCATC TCCTCTCTGG GCATGATAGA AGCCACCGCT 
CAGGTCACCG TCAAAGCTTT GCCAAAGCCT CCCGCCTCCC TCATTGTGAC TGAGACCACA 
GCCACCAGTG TGACTCTCAC CTGGGACTCT GGAAACCCAG AACCTGTCTC CTACTACATG 
ATCCAATACA GAGCCAAGAC CTCGGACAAC GGCTTCCAGG AAGTGGAAGG TGTGGCAACT 
ACCCGTTACA GCATCGGTGG ACTTAGCCCC TACTCTGAGT ATGAGTTTCG TGTCATGGCT 
GTCAACAACA TCGGCCGGGG GCCACCGAGC GACCCCGTGG AGACACGCAC TGGTGAACAG 
GCCCCCTCCT CGCCACCTCT GCACGTCCAA GCGCGCATGC TGAGTGCCAG CACAATGCTG 
GTCCAGTGGG AACCGCCCGA GGAACCCAAC GGGCAAATCA GGGGCTACCG GGTCTACTAC 
AGCTCGGACT TGACGGCTCC GCTCAGCGCC TGGCAGAAGC ACAACACAGA CGACAGCAGC 
CTGACGACCA TCTCGGGCCT GACTCCTGAT ATCACCTACA GCCTCAGGGT GCTGGGCTTC 
ACCTCTGTAG GTGACGGGCC ACCTTCTGAC ATCCTGCAAG TCAAAACCCA ACAGGGAGTC 
CCTGCCCAGC CGTCCAGCTT TGAGGCCGAA GCTGAGCTGG ACAGTCGCAT CATGCTAACA 
TGGCTGTGGC CCGTCCAGGA CCCCATCACT AAGTATGAGC TGCAGTACTG GGAGGCCGGC 
TCAGACAACA AGTTACATGT GAGCTTCAAC CCAGCGGGCT CCTACGCTGT GGACGGTCTA 
AAGCCCGACA CCTTGTACCG TTTCTCTCTG GCAGCCCGTT CTGAGATGGG CTTAGGTGTC 
TACACCCAAC CCATCGAGGC TCGCACCGCC CAGTCCACCC CATCAGCTCC CCCCCAGGAG 
GTACACCTGG TCAGCCTGAG CTCCACCAGC CTCAAGGTGA GTTGGGTGGC ACCGCCCGCC 
GCTAGCCGCC ACGGCGCCAT TGTGCGCTAC ACAGTCAGCT ACCAGGCCAT GGCTGGAGAA 
GACACGGAGC GGCACGAGGT GACGGGCATC GGCGCAGACG CCACTAGCTT TGTGCTGGAG 
GGCCTGGAGA AGTGGACTGA GTATCAGGTG TGGGTGAGGG CACACACTGA CGTGGGCCCC 
GGCCCAGAGA GTGCCCCAGT GAGGATGAGA ACCAAAGAGG ATGTGCCGGG AGCACCCCCC 
AGGAAGCTGG AGGTTGAGGC CATAAACTCC ACAGCAATCC GGGTCACCTG GAAGCCGCCC 
CTGCAGGGGA AGCAGCACGG CCAAATCCGG GGCTATCAAG TCATCTTCTC TCGGCTTGAA 
AACGGCGAAC CACGAGGCCA GCCTAACATC ATGGACGTTG CTTTGCCAGA GGCACAGTGG 
AATATTGAGG AGTCCACCGA GCATGAGGCC ATCATTGCCG GACTGCACTC TGAAACCACC 
TACTCGGTCA CCGTGGCAGC ATACACCACC AAAGGAGACG GAGCGCGCAG CAAAGCCAAA 
GTCATCACCA CGACGGGTGC AGTGCCAGGA AAGCCCACCA TGATGATCAG CACCACTATG 
GGCAACACAG CGCTGATTCA GTGGCAGCCA CCTAAGGAAA TGGTTGGGGA GCTGATGGGC 
TACCAGCTGC AGTACAAGCG CACTGACGAG GAAAACTACA CCTCCCGCGA GCTGAGAAAG 
ATCGATGACC ACTACACCGT CACCGGGCTG CACAAAGGTG CCACCTATGT CTTCCGCCTC 
AGTGCCCGCA ACCGCGCGGG CATCGGCGAA GCATACGTGA AAGAGATCAG CACCCCTGAA 
GACGTGCCGT CGGGCTTCCC GTTCAACCTG CAGGTCACGG GACTGACTCA GTCCAGCACC 
GAGCTGACCT GGGACCCTCC GCTGGTGGCC GAAAGGAACG GCAAGATCGT GCACTATGTG 
GTCGTTTACA GGGACATCAA CAGTCAGCAG AACAGCACCA ACCGGACGAG CGAAACGCAC 
ATGACCATCC AGGGCCTTAA TCCCGACACC ACCTACGATA TCCGGGTGCA GGCGTTCACC 
AGTAAGGGCG GAGGACCCCT CAGCCCTAGC ATCCAGAGCA GGACCATGTC GAACGATTTT 
CCCACCCTCA ACTTTGGTGT CAAAGCCGTC ATGAAAACAT CTGTCCTCCT CACCTGGGAT 
TTGCCACCGA ACTACAAATC CCAAGTGCCT TTCAAGATCC TGTACAACCA GCAAAGCGTG 
GAAGTGCAGG GATCCCTGAA GAGGAGGCTG ATCACGCAGC TGCAGCCAGA CACGGACTAC 
TCTTTTGTGC TGACAAGCAG GGGCAACATT GCTGGTGGTC TACAGCAGCA GGTGTCCATC 
CGCACCGCTC CCGACCTGAT CAACGCCAAG CCCAGCACAC ATCAGCCTCA GGGTAGCAAG 
ATGACAATCA ACCTGCCCAA GGTCCAGACC ACCACGCGCG TCAGGTGGTA CTACATCGTG 
GTGGTGCCGG TGTCCCAGTC AACGCAGGGG TGGAAGAATC CAGACGAGAT GGACCTGGAC 
GAGCTGTTCA AGTCAAGTGA AGGCCCCGTC CTGAGGCGCA GACGTCACCT GGATTCCTCC 
AAACCTTACA TCGCTGCCAA GTTGGCCTCG TTGCCGACCA CCTTCACCCT GGGTGATGAG 
AAGAGATACA ACGGCTTCTA CAACAAGCCC CTGAGCAGTC CGCAGGAGTA TCTCTGCTTT 
GTCCTGGCAG TACTCCGATC CGAAGGGGCA GACAGCACTA CTAACTATAT GACATTTTCA 
GCCAGTCCCT ACTCAGACCC GGTCCACGTG CGAAACAGCA TGGCTCAGGG GATGGAGCAG 
CCTGAAATGT TGTGGGTTAT GGGCCCTGTT TTGGCCGTGG TCCTGATTAT CATCATCGTC 
ATCGCCATCC TGCTCTTCAA GAACAAACAG GAAAGGAAGC GAGCGTCGCC CCTGCCCAAA 
GATGAGCACT CGGGCGGGGT GAAGGACTCC CTGCTGGCCA ACTCCTCCGA CCCTGTGGAG 
ATGAGAAGAC TTAACTACCA GACCCCAGGT CCCAGCTCTC ATCGCTGCCC CAACACTCCA 
AGAATGAGGG AGCACCCGCC CATCCCTGTC GTCGACCTGG CTGACCACAT AGAGCGTCTG 
AAGGCCAACG ACGGCCTACG CTTTTCCCAG GAGTACGAGT CGATTGATCC AGGGCAGCAG 
TTTACCTGGG AGAACTCCAA CATGGAAGTC AACAAGCCAA AGAATCGCTA TGCAAACGTC 
ATTGCCTATG ACCACTCCAG AGTGGTTCTC ACCTCTGTTG ACGCCGTTCC AGGCAGTGAC 
TACATCAACG CCAACTACAT CGACGGCTAC AGGAAGCAGA ACGCCTACAT CGCCACGCAG 
GGGCCACTTC CAGAGACACT GAGCGACTTC TGGAGGATGG TGTGGGAGCA AAGATCCAAC 
ACCATCGTCA TGATGACCAG ACTGGAGGAG AAGTCACGGG TGAAGTGTGA CCAGTACTGG 
CCGTCGCGAG GAACGGAGAC CTATGGGATG ATCCAGGTCA CCATGCTGGA CACCGTGGAG 
TTGGCCACTT ACAGCGTCCG TACCTTTGCC CTCTATAAGA ATGGCTCCAG TGAGAAGAGA 
GAGGTGAGAC AGTTCCAGTT CATGGCCTGG CCAGACCACG GGGTGCCAGA GTACCCCACC 
CCAATCCTGG CCTTCCTACG GCGGGTAAAG GCCTGCAATC CTCCAGACGC AGGGCCTATG 
GTGGTCCACT GCAGCGCGGG TGTGGGCAGG ACCGGATGCT TCATCGTGAT AGATGCCATG 
CTGGAGCGCA TGAAGCACGA GAAGTCCGTA GACATCTACG GCCACGTGAC GTGCATGCGC 
GCCCAGAGGA ACTACATGGT CCAGACCGAG GACCAGTACA TCTTCATTCA CGAAGCCCTG 
CTGGAGGCCG CCACGTGCGG CAACACCGAG GTGCCGGCGC GCAACCTGTA CGCCCACATC 
CAGAAACTCA CCCAGCCCCC GCCCGGCGAG ACGGTTACTG CCATGGAGCT GGAATTCAAG 
AGACTGGCCA ACTCCAAAGC CCACACATCC CGCTTCATCA GCGCCAACTT GCCCTGCAAC 
AAGTTCAAGA ACCGCCTGGT CAACATCATG CCTTTCGAGT CCACCCGTGT CTGCCTGCAG 
CCCATCCGTG GCGTCGAGGG CTCCGACTAC ATCAATGCCA GCTGCATCGA CGGCTACAGA 
CAGCAGAAGG CCTACCTGGC CACGCAGGGC CCACTGGCAG AGACCACCGA GGACTTCTGG 
AGGATGCTGT GGGAGCACAA CTCCACCATC GTCGTCATGC TCACCAAGCT ACGAGAGATG 
GGCCGGGAGA AGTGCCATCA GTACTGGCCA GCAGAGCGTT CAGCCAGGTA CCAGTACTTT 
GTGGTGGATC CAATGGCTGA GTACAACATG CCCCAGTACA TCCTCAGAGA GTTCAAAGTC 
ACAGATGCCA GGGACGGCCA GTCCAGAACA ATCCGCCAAT TCCAGTTCAC TGACTGGCCC 
GAGCAAGGAG TGCCAAAAAC CGGAGAGGGC TTCATTGATT TTATCGGACA AGTGCACAAA 
ACCAAGGAAC AGTTCGGACA GGACGGACCA ATCACTGTAC ACTGCAGTGC TGGGGTGGGC 
AGGACCGGAG TCTTCATCAC CCTCAGCATC GTGCTGGAGA GGATGAGGTA TGAAGGAGTT 
GTCGACCTCT TCCAGACAGT GAAGACACTT CGCACCCAGC GGCCTGCCAT GGTGCAGACC 
GAGGACCAGT ACCAGTTGTG CTACAGGGCA GCATTGGAGT ACCTGGGGAG CTTTGACCAC 
TATGCAACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104931439 ( XM_019263532.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019119077.1 Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X3, mRNA.

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CloneID	OLa84597
Clone ID Related Accession (Same CDS sequence)	XM_019263532.1
Accession Version	XM_019263532.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	receptor-type tyrosine-protein phosphatase F isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017607936.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTTGACATC 
AGCAGCAGCA ACGGACGCAT TAAACAGCTT CGTTCAGGTG GTACACCAAT TAGAGGAGCT 
CTGCAAATTG AGAACAGCGA GGAGTCCGAC CAAGGGAAAT ACGAGTGTGT TGCCATGAAC 
AGCGCTGGGA CCCGTTACTC CGCTCCTGCC AATCTCTACG TACGAGTGCG AAGAGTCCCA 
CCCAGATTCT CCATTCCGCC CACCAACCAA GAGGTGATGC CAGGCGGCAG CGTCAACCTG 
ACATGCGTGG CAGTGGGCTC GCCGATGCCC TACGTCAAGT GGACGATGGG CCTGGTGGAG 
CTGACCAAGG AGGAGGAGAT GCCGATGGGA CGCAACGTGC TGGAGGTCAA CAACATCCGC 
GAGTCGGCGA ACTACACCTG CGTGGCCATC TCCTCTCTGG GCATGATAGA AGCCACCGCT 
CAGGTCACCG TCAAAGCTTT GCCAAAGCCT CCCGCCTCCC TCATTGTGAC TGAGACCACA 
GCCACCAGTG TGACTCTCAC CTGGGACTCT GGAAACCCAG AACCTGTCTC CTACTACATG 
ATCCAATACA GAGCCAAGAC CTCGGACAAC GGCTTCCAGG AAGTGGAAGG TGTGGCAACT 
ACCCGTTACA GCATCGGTGG ACTTAGCCCC TACTCTGAGT ATGAGTTTCG TGTCATGGCT 
GTCAACAACA TCGGCCGGGG GCCACCGAGC GACCCCGTGG AGACACGCAC TGGTGAACAG 
GCCCCCTCCT CGCCACCTCT GCACGTCCAA GCGCGCATGC TGAGTGCCAG CACAATGCTG 
GTCCAGTGGG AACCGCCCGA GGAACCCAAC GGGCAAATCA GGGGCTACCG GGTCTACTAC 
AGCTCGGACT TGACGGCTCC GCTCAGCGCC TGGCAGAAGC ACAACACAGA CGACAGCAGC 
CTGACGACCA TCTCGGGCCT GACTCCTGAT ATCACCTACA GCCTCAGGGT GCTGGGCTTC 
ACCTCTGTAG GTGACGGGCC ACCTTCTGAC ATCCTGCAAG TCAAAACCCA ACAGGGAGTC 
CCTGCCCAGC CGTCCAGCTT TGAGGCCGAA GCTGAGCTGG ACAGTCGCAT CATGCTAACA 
TGGCTGTGGC CCGTCCAGGA CCCCATCACT AAGTATGAGC TGCAGTACTG GGAGGCCGGC 
TCAGACAACA AGTTACATGT GAGCTTCAAC CCAGCGGGCT CCTACGCTGT GGACGGTCTA 
AAGCCCGACA CCTTGTACCG TTTCTCTCTG GCAGCCCGTT CTGAGATGGG CTTAGGTGTC 
TACACCCAAC CCATCGAGGC TCGCACCGCC CAGTCCACCC CATCAGCTCC CCCCCAGGAG 
GTACACCTGG TCAGCCTGAG CTCCACCAGC CTCAAGGTGA GTTGGGTGGC ACCGCCCGCC 
GCTAGCCGCC ACGGCGCCAT TGTGCGCTAC ACAGTCAGCT ACCAGGCCAT GGCTGGAGAA 
GACACGGAGC GGCACGAGGT GACGGGCATC GGCGCAGACG CCACTAGCTT TGTGCTGGAG 
GGCCTGGAGA AGTGGACTGA GTATCAGGTG TGGGTGAGGG CACACACTGA CGTGGGCCCC 
GGCCCAGAGA GTGCCCCAGT GAGGATGAGA ACCAAAGAGG ATGTGCCGGG AGCACCCCCC 
AGGAAGCTGG AGGTTGAGGC CATAAACTCC ACAGCAATCC GGGTCACCTG GAAGCCGCCC 
CTGCAGGGGA AGCAGCACGG CCAAATCCGG GGCTATCAAG TCATCTTCTC TCGGCTTGAA 
AACGGCGAAC CACGAGGCCA GCCTAACATC ATGGACGTTG CTTTGCCAGA GGCACAGTGG 
AATATTGAGG AGTCCACCGA GCATGAGGCC ATCATTGCCG GACTGCACTC TGAAACCACC 
TACTCGGTCA CCGTGGCAGC ATACACCACC AAAGGAGACG GAGCGCGCAG CAAAGCCAAA 
GTCATCACCA CGACGGGTGC AGTGCCAGGA AAGCCCACCA TGATGATCAG CACCACTATG 
GGCAACACAG CGCTGATTCA GTGGCAGCCA CCTAAGGAAA TGGTTGGGGA GCTGATGGGC 
TACCAGCTGC AGTACAAGCG CACTGACGAG GAAAACTACA CCTCCCGCGA GCTGAGAAAG 
ATCGATGACC ACTACACCGT CACCGGGCTG CACAAAGGTG CCACCTATGT CTTCCGCCTC 
AGTGCCCGCA ACCGCGCGGG CATCGGCGAA GCATACGTGA AAGAGATCAG CACCCCTGAA 
GACGTGCCGT CGGGCTTCCC GTTCAACCTG CAGGTCACGG GACTGACTCA GTCCAGCACC 
GAGCTGACCT GGGACCCTCC GCTGGTGGCC GAAAGGAACG GCAAGATCGT GCACTATGTG 
GTCGTTTACA GGGACATCAA CAGTCAGCAG AACAGCACCA ACCGGACGAG CGAAACGCAC 
ATGACCATCC AGGGCCTTAA TCCCGACACC ACCTACGATA TCCGGGTGCA GGCGTTCACC 
AGTAAGGGCG GAGGACCCCT CAGCCCTAGC ATCCAGAGCA GGACCATGTC GAACGATTTT 
CCCACCCTCA ACTTTGGTGT CAAAGCCGTC ATGAAAACAT CTGTCCTCCT CACCTGGGAT 
TTGCCACCGA ACTACAAATC CCAAGTGCCT TTCAAGATCC TGTACAACCA GCAAAGCGTG 
GAAGTGCAGG GATCCCTGAA GAGGAGGCTG ATCACGCAGC TGCAGCCAGA CACGGACTAC 
TCTTTTGTGC TGACAAGCAG GGGCAACATT GCTGGTGGTC TACAGCAGCA GGTGTCCATC 
CGCACCGCTC CCGACCTGAT CAACGCCAAG CCCAGCACAC ATCAGCCTCA GGGTAGCAAG 
ATGACAATCA ACCTGCCCAA GGTCCAGACC ACCACGCGCG TCAGGTGGTA CTACATCGTG 
GTGGTGCCGG TGTCCCAGTC AACGCAGGGG TGGAAGAATC CAGACGAGAT GGACCTGGAC 
GAGCTGTTCA AGTCAAGTGA AGGCCCCGTC CTGAGGCGCA GACGTCACCT GGATTCCTCC 
AAACCTTACA TCGCTGCCAA GTTGGCCTCG TTGCCGACCA CCTTCACCCT GGGTGATGAG 
AAGAGATACA ACGGCTTCTA CAACAAGCCC CTGAGCAGTC CGCAGGAGTA TCTCTGCTTT 
GTCCTGGCAG TACTCCGATC CGAAGGGGCA GACAGCACTA CTAACTATAT GACATTTTCA 
GCCAGTCCCT ACTCAGACCC GGTCCACGTG CGAAACAGCA TGGCTCAGGG GATGGAGCAG 
CCTGAAATGT TGTGGGTTAT GGGCCCTGTT TTGGCCGTGG TCCTGATTAT CATCATCGTC 
ATCGCCATCC TGCTCTTCAA GAAGAAGCGA GCGTCGCCCC TGCCCAAAGA TGAGCACTCG 
GGCGGGGTGA AGGACTCCCT GCTGGCCAAC TCCTCCGACC CTGTGGAGAT GAGAAGACTT 
AACTACCAGA CCCCAGGTCC CAGCTCTCAT CGCTGCCCCA ACACTCCAAG AATGAGGGAG 
CACCCGCCCA TCCCTGTCGT CGACCTGGCT GACCACATAG AGCGTCTGAA GGCCAACGAC 
GGCCTACGCT TTTCCCAGGA GTACGAGTCG ATTGATCCAG GGCAGCAGTT TACCTGGGAG 
AACTCCAACA TGGAAGTCAA CAAGCCAAAG AATCGCTATG CAAACGTCAT TGCCTATGAC 
CACTCCAGAG TGGTTCTCAC CTCTGTTGAC GCCGTTCCAG GCAGTGACTA CATCAACGCC 
AACTACATCG ACGGCTACAG GAAGCAGAAC GCCTACATCG CCACGCAGGG GCCACTTCCA 
GAGACACTGA GCGACTTCTG GAGGATGGTG TGGGAGCAAA GATCCAACAC CATCGTCATG 
ATGACCAGAC TGGAGGAGAA GTCACGGGTG AAGTGTGACC AGTACTGGCC GTCGCGAGGA 
ACGGAGACCT ATGGGATGAT CCAGGTCACC ATGCTGGACA CCGTGGAGTT GGCCACTTAC 
AGCGTCCGTA CCTTTGCCCT CTATAAGAAT GGCTCCAGTG AGAAGAGAGA GGTGAGACAG 
TTCCAGTTCA TGGCCTGGCC AGACCACGGG GTGCCAGAGT ACCCCACCCC AATCCTGGCC 
TTCCTACGGC GGGTAAAGGC CTGCAATCCT CCAGACGCAG GGCCTATGGT GGTCCACTGC 
AGCGCGGGTG TGGGCAGGAC CGGATGCTTC ATCGTGATAG ATGCCATGCT GGAGCGCATG 
AAGCACGAGA AGTCCGTAGA CATCTACGGC CACGTGACGT GCATGCGCGC CCAGAGGAAC 
TACATGGTCC AGACCGAGGA CCAGTACATC TTCATTCACG AAGCCCTGCT GGAGGCCGCC 
ACGTGCGGCA ACACCGAGGT GCCGGCGCGC AACCTGTACG CCCACATCCA GAAACTCACC 
CAGCCCCCGC CCGGCGAGAC GGTTACTGCC ATGGAGCTGG AATTCAAGAG ACTGGCCAAC 
TCCAAAGCCC ACACATCCCG CTTCATCAGC GCCAACTTGC CCTGCAACAA GTTCAAGAAC 
CGCCTGGTCA ACATCATGCC TTTCGAGTCC ACCCGTGTCT GCCTGCAGCC CATCCGTGGC 
GTCGAGGGCT CCGACTACAT CAATGCCAGC TGCATCGACG GCTACAGACA GCAGAAGGCC 
TACCTGGCCA CGCAGGGCCC ACTGGCAGAG ACCACCGAGG ACTTCTGGAG GATGCTGTGG 
GAGCACAACT CCACCATCGT CGTCATGCTC ACCAAGCTAC GAGAGATGGG CCGGGAGAAG 
TGCCATCAGT ACTGGCCAGC AGAGCGTTCA GCCAGGTACC AGTACTTTGT GGTGGATCCA 
ATGGCTGAGT ACAACATGCC CCAGTACATC CTCAGAGAGT TCAAAGTCAC AGATGCCAGG 
GACGGCCAGT CCAGAACAAT CCGCCAATTC CAGTTCACTG ACTGGCCCGA GCAAGGAGTG 
CCAAAAACCG GAGAGGGCTT CATTGATTTT ATCGGACAAG TGCACAAAAC CAAGGAACAG 
TTCGGACAGG ACGGACCAAT CACTGTACAC TGCAGTGCTG GGGTGGGCAG GACCGGAGTC 
TTCATCACCC TCAGCATCGT GCTGGAGAGG ATGAGGTATG AAGGAGTTGT CGACCTCTTC 
CAGACAGTGA AGACACTTCG CACCCAGCGG CCTGCCATGG TGCAGACCGA GGACCAGTAC 
CAGTTGTGCT ACAGGGCAGC ATTGGAGTAC CTGGGGAGCT TTGACCACTA TGCAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019119077.1
CDS	523..6282
Translation	MVPNIHLAVPFLPRGALLFLILLSCVVFSSLADSMPSFVKSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVEKTRTATMLCAASGNPDPEISWFKDMLPVDISSSNGRIKQLRSGGTPIRGALQIENSEESDQGKYECVAMNSAGTRYSAPANLYVRVRRVPPRFSIPPTNQEVMPGGSVNLTCVAVGSPMPYVKWTMGLVELTKEEEMPMGRNVLEVNNIRESANYTCVAISSLGMIEATAQVTVKALPKPPASLIVTETTATSVTLTWDSGNPEPVSYYMIQYRAKTSDNGFQEVEGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSDPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRVYYSSDLTAPLSAWQKHNTDDSSLTTISGLTPDITYSLRVLGFTSVGDGPPSDILQVKTQQGVPAQPSSFEAEAELDSRIMLTWLWPVQDPITKYELQYWEAGSDNKLHVSFNPAGSYAVDGLKPDTLYRFSLAARSEMGLGVYTQPIEARTAQSTPSAPPQEVHLVSLSSTSLKVSWVAPPAASRHGAIVRYTVSYQAMAGEDTERHEVTGIGADATSFVLEGLEKWTEYQVWVRAHTDVGPGPESAPVRMRTKEDVPGAPPRKLEVEAINSTAIRVTWKPPLQGKQHGQIRGYQVIFSRLENGEPRGQPNIMDVALPEAQWNIEESTEHEAIIAGLHSETTYSVTVAAYTTKGDGARSKAKVITTTGAVPGKPTMMISTTMGNTALIQWQPPKEMVGELMGYQLQYKRTDEENYTSRELRKIDDHYTVTGLHKGATYVFRLSARNRAGIGEAYVKEISTPEDVPSGFPFNLQVTGLTQSSTELTWDPPLVAERNGKIVHYVVVYRDINSQQNSTNRTSETHMTIQGLNPDTTYDIRVQAFTSKGGGPLSPSIQSRTMSNDFPTLNFGVKAVMKTSVLLTWDLPPNYKSQVPFKILYNQQSVEVQGSLKRRLITQLQPDTDYSFVLTSRGNIAGGLQQQVSIRTAPDLINAKPSTHQPQGSKMTINLPKVQTTTRVRWYYIVVVPVSQSTQGWKNPDEMDLDELFKSSEGPVLRRRRHLDSSKPYIAAKLASLPTTFTLGDEKRYNGFYNKPLSSPQEYLCFVLAVLRSEGADSTTNYMTFSASPYSDPVHVRNSMAQGMEQPEMLWVMGPVLAVVLIIIIVIAILLFKKKRASPLPKDEHSGGVKDSLLANSSDPVEMRRLNYQTPGPSSHRCPNTPRMREHPPIPVVDLADHIERLKANDGLRFSQEYESIDPGQQFTWENSNMEVNKPKNRYANVIAYDHSRVVLTSVDAVPGSDYINANYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRSNTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVTMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRAQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPPPGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYLATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_019263532.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019263532.1

ORF Insert Sequence:

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTTGACATC 
AGCAGCAGCA ACGGACGCAT TAAACAGCTT CGTTCAGGTG GTACACCAAT TAGAGGAGCT 
CTGCAAATTG AGAACAGCGA GGAGTCCGAC CAAGGGAAAT ACGAGTGTGT TGCCATGAAC 
AGCGCTGGGA CCCGTTACTC CGCTCCTGCC AATCTCTACG TACGAGTGCG AAGAGTCCCA 
CCCAGATTCT CCATTCCGCC CACCAACCAA GAGGTGATGC CAGGCGGCAG CGTCAACCTG 
ACATGCGTGG CAGTGGGCTC GCCGATGCCC TACGTCAAGT GGACGATGGG CCTGGTGGAG 
CTGACCAAGG AGGAGGAGAT GCCGATGGGA CGCAACGTGC TGGAGGTCAA CAACATCCGC 
GAGTCGGCGA ACTACACCTG CGTGGCCATC TCCTCTCTGG GCATGATAGA AGCCACCGCT 
CAGGTCACCG TCAAAGCTTT GCCAAAGCCT CCCGCCTCCC TCATTGTGAC TGAGACCACA 
GCCACCAGTG TGACTCTCAC CTGGGACTCT GGAAACCCAG AACCTGTCTC CTACTACATG 
ATCCAATACA GAGCCAAGAC CTCGGACAAC GGCTTCCAGG AAGTGGAAGG TGTGGCAACT 
ACCCGTTACA GCATCGGTGG ACTTAGCCCC TACTCTGAGT ATGAGTTTCG TGTCATGGCT 
GTCAACAACA TCGGCCGGGG GCCACCGAGC GACCCCGTGG AGACACGCAC TGGTGAACAG 
GCCCCCTCCT CGCCACCTCT GCACGTCCAA GCGCGCATGC TGAGTGCCAG CACAATGCTG 
GTCCAGTGGG AACCGCCCGA GGAACCCAAC GGGCAAATCA GGGGCTACCG GGTCTACTAC 
AGCTCGGACT TGACGGCTCC GCTCAGCGCC TGGCAGAAGC ACAACACAGA CGACAGCAGC 
CTGACGACCA TCTCGGGCCT GACTCCTGAT ATCACCTACA GCCTCAGGGT GCTGGGCTTC 
ACCTCTGTAG GTGACGGGCC ACCTTCTGAC ATCCTGCAAG TCAAAACCCA ACAGGGAGTC 
CCTGCCCAGC CGTCCAGCTT TGAGGCCGAA GCTGAGCTGG ACAGTCGCAT CATGCTAACA 
TGGCTGTGGC CCGTCCAGGA CCCCATCACT AAGTATGAGC TGCAGTACTG GGAGGCCGGC 
TCAGACAACA AGTTACATGT GAGCTTCAAC CCAGCGGGCT CCTACGCTGT GGACGGTCTA 
AAGCCCGACA CCTTGTACCG TTTCTCTCTG GCAGCCCGTT CTGAGATGGG CTTAGGTGTC 
TACACCCAAC CCATCGAGGC TCGCACCGCC CAGTCCACCC CATCAGCTCC CCCCCAGGAG 
GTACACCTGG TCAGCCTGAG CTCCACCAGC CTCAAGGTGA GTTGGGTGGC ACCGCCCGCC 
GCTAGCCGCC ACGGCGCCAT TGTGCGCTAC ACAGTCAGCT ACCAGGCCAT GGCTGGAGAA 
GACACGGAGC GGCACGAGGT GACGGGCATC GGCGCAGACG CCACTAGCTT TGTGCTGGAG 
GGCCTGGAGA AGTGGACTGA GTATCAGGTG TGGGTGAGGG CACACACTGA CGTGGGCCCC 
GGCCCAGAGA GTGCCCCAGT GAGGATGAGA ACCAAAGAGG ATGTGCCGGG AGCACCCCCC 
AGGAAGCTGG AGGTTGAGGC CATAAACTCC ACAGCAATCC GGGTCACCTG GAAGCCGCCC 
CTGCAGGGGA AGCAGCACGG CCAAATCCGG GGCTATCAAG TCATCTTCTC TCGGCTTGAA 
AACGGCGAAC CACGAGGCCA GCCTAACATC ATGGACGTTG CTTTGCCAGA GGCACAGTGG 
AATATTGAGG AGTCCACCGA GCATGAGGCC ATCATTGCCG GACTGCACTC TGAAACCACC 
TACTCGGTCA CCGTGGCAGC ATACACCACC AAAGGAGACG GAGCGCGCAG CAAAGCCAAA 
GTCATCACCA CGACGGGTGC AGTGCCAGGA AAGCCCACCA TGATGATCAG CACCACTATG 
GGCAACACAG CGCTGATTCA GTGGCAGCCA CCTAAGGAAA TGGTTGGGGA GCTGATGGGC 
TACCAGCTGC AGTACAAGCG CACTGACGAG GAAAACTACA CCTCCCGCGA GCTGAGAAAG 
ATCGATGACC ACTACACCGT CACCGGGCTG CACAAAGGTG CCACCTATGT CTTCCGCCTC 
AGTGCCCGCA ACCGCGCGGG CATCGGCGAA GCATACGTGA AAGAGATCAG CACCCCTGAA 
GACGTGCCGT CGGGCTTCCC GTTCAACCTG CAGGTCACGG GACTGACTCA GTCCAGCACC 
GAGCTGACCT GGGACCCTCC GCTGGTGGCC GAAAGGAACG GCAAGATCGT GCACTATGTG 
GTCGTTTACA GGGACATCAA CAGTCAGCAG AACAGCACCA ACCGGACGAG CGAAACGCAC 
ATGACCATCC AGGGCCTTAA TCCCGACACC ACCTACGATA TCCGGGTGCA GGCGTTCACC 
AGTAAGGGCG GAGGACCCCT CAGCCCTAGC ATCCAGAGCA GGACCATGTC GAACGATTTT 
CCCACCCTCA ACTTTGGTGT CAAAGCCGTC ATGAAAACAT CTGTCCTCCT CACCTGGGAT 
TTGCCACCGA ACTACAAATC CCAAGTGCCT TTCAAGATCC TGTACAACCA GCAAAGCGTG 
GAAGTGCAGG GATCCCTGAA GAGGAGGCTG ATCACGCAGC TGCAGCCAGA CACGGACTAC 
TCTTTTGTGC TGACAAGCAG GGGCAACATT GCTGGTGGTC TACAGCAGCA GGTGTCCATC 
CGCACCGCTC CCGACCTGAT CAACGCCAAG CCCAGCACAC ATCAGCCTCA GGGTAGCAAG 
ATGACAATCA ACCTGCCCAA GGTCCAGACC ACCACGCGCG TCAGGTGGTA CTACATCGTG 
GTGGTGCCGG TGTCCCAGTC AACGCAGGGG TGGAAGAATC CAGACGAGAT GGACCTGGAC 
GAGCTGTTCA AGTCAAGTGA AGGCCCCGTC CTGAGGCGCA GACGTCACCT GGATTCCTCC 
AAACCTTACA TCGCTGCCAA GTTGGCCTCG TTGCCGACCA CCTTCACCCT GGGTGATGAG 
AAGAGATACA ACGGCTTCTA CAACAAGCCC CTGAGCAGTC CGCAGGAGTA TCTCTGCTTT 
GTCCTGGCAG TACTCCGATC CGAAGGGGCA GACAGCACTA CTAACTATAT GACATTTTCA 
GCCAGTCCCT ACTCAGACCC GGTCCACGTG CGAAACAGCA TGGCTCAGGG GATGGAGCAG 
CCTGAAATGT TGTGGGTTAT GGGCCCTGTT TTGGCCGTGG TCCTGATTAT CATCATCGTC 
ATCGCCATCC TGCTCTTCAA GAAGAAGCGA GCGTCGCCCC TGCCCAAAGA TGAGCACTCG 
GGCGGGGTGA AGGACTCCCT GCTGGCCAAC TCCTCCGACC CTGTGGAGAT GAGAAGACTT 
AACTACCAGA CCCCAGGTCC CAGCTCTCAT CGCTGCCCCA ACACTCCAAG AATGAGGGAG 
CACCCGCCCA TCCCTGTCGT CGACCTGGCT GACCACATAG AGCGTCTGAA GGCCAACGAC 
GGCCTACGCT TTTCCCAGGA GTACGAGTCG ATTGATCCAG GGCAGCAGTT TACCTGGGAG 
AACTCCAACA TGGAAGTCAA CAAGCCAAAG AATCGCTATG CAAACGTCAT TGCCTATGAC 
CACTCCAGAG TGGTTCTCAC CTCTGTTGAC GCCGTTCCAG GCAGTGACTA CATCAACGCC 
AACTACATCG ACGGCTACAG GAAGCAGAAC GCCTACATCG CCACGCAGGG GCCACTTCCA 
GAGACACTGA GCGACTTCTG GAGGATGGTG TGGGAGCAAA GATCCAACAC CATCGTCATG 
ATGACCAGAC TGGAGGAGAA GTCACGGGTG AAGTGTGACC AGTACTGGCC GTCGCGAGGA 
ACGGAGACCT ATGGGATGAT CCAGGTCACC ATGCTGGACA CCGTGGAGTT GGCCACTTAC 
AGCGTCCGTA CCTTTGCCCT CTATAAGAAT GGCTCCAGTG AGAAGAGAGA GGTGAGACAG 
TTCCAGTTCA TGGCCTGGCC AGACCACGGG GTGCCAGAGT ACCCCACCCC AATCCTGGCC 
TTCCTACGGC GGGTAAAGGC CTGCAATCCT CCAGACGCAG GGCCTATGGT GGTCCACTGC 
AGCGCGGGTG TGGGCAGGAC CGGATGCTTC ATCGTGATAG ATGCCATGCT GGAGCGCATG 
AAGCACGAGA AGTCCGTAGA CATCTACGGC CACGTGACGT GCATGCGCGC CCAGAGGAAC 
TACATGGTCC AGACCGAGGA CCAGTACATC TTCATTCACG AAGCCCTGCT GGAGGCCGCC 
ACGTGCGGCA ACACCGAGGT GCCGGCGCGC AACCTGTACG CCCACATCCA GAAACTCACC 
CAGCCCCCGC CCGGCGAGAC GGTTACTGCC ATGGAGCTGG AATTCAAGAG ACTGGCCAAC 
TCCAAAGCCC ACACATCCCG CTTCATCAGC GCCAACTTGC CCTGCAACAA GTTCAAGAAC 
CGCCTGGTCA ACATCATGCC TTTCGAGTCC ACCCGTGTCT GCCTGCAGCC CATCCGTGGC 
GTCGAGGGCT CCGACTACAT CAATGCCAGC TGCATCGACG GCTACAGACA GCAGAAGGCC 
TACCTGGCCA CGCAGGGCCC ACTGGCAGAG ACCACCGAGG ACTTCTGGAG GATGCTGTGG 
GAGCACAACT CCACCATCGT CGTCATGCTC ACCAAGCTAC GAGAGATGGG CCGGGAGAAG 
TGCCATCAGT ACTGGCCAGC AGAGCGTTCA GCCAGGTACC AGTACTTTGT GGTGGATCCA 
ATGGCTGAGT ACAACATGCC CCAGTACATC CTCAGAGAGT TCAAAGTCAC AGATGCCAGG 
GACGGCCAGT CCAGAACAAT CCGCCAATTC CAGTTCACTG ACTGGCCCGA GCAAGGAGTG 
CCAAAAACCG GAGAGGGCTT CATTGATTTT ATCGGACAAG TGCACAAAAC CAAGGAACAG 
TTCGGACAGG ACGGACCAAT CACTGTACAC TGCAGTGCTG GGGTGGGCAG GACCGGAGTC 
TTCATCACCC TCAGCATCGT GCTGGAGAGG ATGAGGTATG AAGGAGTTGT CGACCTCTTC 
CAGACAGTGA AGACACTTCG CACCCAGCGG CCTGCCATGG TGCAGACCGA GGACCAGTAC 
CAGTTGTGCT ACAGGGCAGC ATTGGAGTAC CTGGGGAGCT TTGACCACTA TGCAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104931439 ( XM_010746444.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010744746.2 Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X4, mRNA.

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CloneID	OLa84598
Clone ID Related Accession (Same CDS sequence)	XM_010746444.2
Accession Version	XM_010746444.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	receptor-type tyrosine-protein phosphatase F isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017607936.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 29, 2016 this sequence version replaced XM_010746444.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTTGACATC 
AGCAGCAGCA ACGGACGCAT TAAACAGCTT CGTTCAGGAG CTCTGCAAAT TGAGAACAGC 
GAGGAGTCCG ACCAAGGGAA ATACGAGTGT GTTGCCATGA ACAGCGCTGG GACCCGTTAC 
TCCGCTCCTG CCAATCTCTA CGTACGAGTG CGAAGAGTCC CACCCAGATT CTCCATTCCG 
CCCACCAACC AAGAGGTGAT GCCAGGCGGC AGCGTCAACC TGACATGCGT GGCAGTGGGC 
TCGCCGATGC CCTACGTCAA GTGGACGATG GGCCTGGTGG AGCTGACCAA GGAGGAGGAG 
ATGCCGATGG GACGCAACGT GCTGGAGGTC AACAACATCC GCGAGTCGGC GAACTACACC 
TGCGTGGCCA TCTCCTCTCT GGGCATGATA GAAGCCACCG CTCAGGTCAC CGTCAAAGCT 
TTGCCAAAGC CTCCCGCCTC CCTCATTGTG ACTGAGACCA CAGCCACCAG TGTGACTCTC 
ACCTGGGACT CTGGAAACCC AGAACCTGTC TCCTACTACA TGATCCAATA CAGAGCCAAG 
ACCTCGGACA ACGGCTTCCA GGAAGTGGAA GGTGTGGCAA CTACCCGTTA CAGCATCGGT 
GGACTTAGCC CCTACTCTGA GTATGAGTTT CGTGTCATGG CTGTCAACAA CATCGGCCGG 
GGGCCACCGA GCGACCCCGT GGAGACACGC ACTGGTGAAC AGGCCCCCTC CTCGCCACCT 
CTGCACGTCC AAGCGCGCAT GCTGAGTGCC AGCACAATGC TGGTCCAGTG GGAACCGCCC 
GAGGAACCCA ACGGGCAAAT CAGGGGCTAC CGGGTCTACT ACAGCTCGGA CTTGACGGCT 
CCGCTCAGCG CCTGGCAGAA GCACAACACA GACGACAGCA GCCTGACGAC CATCTCGGGC 
CTGACTCCTG ATATCACCTA CAGCCTCAGG GTGCTGGGCT TCACCTCTGT AGGTGACGGG 
CCACCTTCTG ACATCCTGCA AGTCAAAACC CAACAGGGAG TCCCTGCCCA GCCGTCCAGC 
TTTGAGGCCG AAGCTGAGCT GGACAGTCGC ATCATGCTAA CATGGCTGTG GCCCGTCCAG 
GACCCCATCA CTAAGTATGA GCTGCAGTAC TGGGAGGCCG GCTCAGACAA CAAGTTACAT 
GTGAGCTTCA ACCCAGCGGG CTCCTACGCT GTGGACGGTC TAAAGCCCGA CACCTTGTAC 
CGTTTCTCTC TGGCAGCCCG TTCTGAGATG GGCTTAGGTG TCTACACCCA ACCCATCGAG 
GCTCGCACCG CCCAGTCCAC CCCATCAGCT CCCCCCCAGG AGGTACACCT GGTCAGCCTG 
AGCTCCACCA GCCTCAAGGT GAGTTGGGTG GCACCGCCCG CCGCTAGCCG CCACGGCGCC 
ATTGTGCGCT ACACAGTCAG CTACCAGGCC ATGGCTGGAG AAGACACGGA GCGGCACGAG 
GTGACGGGCA TCGGCGCAGA CGCCACTAGC TTTGTGCTGG AGGGCCTGGA GAAGTGGACT 
GAGTATCAGG TGTGGGTGAG GGCACACACT GACGTGGGCC CCGGCCCAGA GAGTGCCCCA 
GTGAGGATGA GAACCAAAGA GGATGTGCCG GGAGCACCCC CCAGGAAGCT GGAGGTTGAG 
GCCATAAACT CCACAGCAAT CCGGGTCACC TGGAAGCCGC CCCTGCAGGG GAAGCAGCAC 
GGCCAAATCC GGGGCTATCA AGTCATCTTC TCTCGGCTTG AAAACGGCGA ACCACGAGGC 
CAGCCTAACA TCATGGACGT TGCTTTGCCA GAGGCACAGT GGAATATTGA GGAGTCCACC 
GAGCATGAGG CCATCATTGC CGGACTGCAC TCTGAAACCA CCTACTCGGT CACCGTGGCA 
GCATACACCA CCAAAGGAGA CGGAGCGCGC AGCAAAGCCA AAGTCATCAC CACGACGGGT 
GCAGTGCCAG GAAAGCCCAC CATGATGATC AGCACCACTA TGGGCAACAC AGCGCTGATT 
CAGTGGCAGC CACCTAAGGA AATGGTTGGG GAGCTGATGG GCTACCAGCT GCAGTACAAG 
CGCACTGACG AGGAAAACTA CACCTCCCGC GAGCTGAGAA AGATCGATGA CCACTACACC 
GTCACCGGGC TGCACAAAGG TGCCACCTAT GTCTTCCGCC TCAGTGCCCG CAACCGCGCG 
GGCATCGGCG AAGCATACGT GAAAGAGATC AGCACCCCTG AAGACGTGCC GTCGGGCTTC 
CCGTTCAACC TGCAGGTCAC GGGACTGACT CAGTCCAGCA CCGAGCTGAC CTGGGACCCT 
CCGCTGGTGG CCGAAAGGAA CGGCAAGATC GTGCACTATG TGGTCGTTTA CAGGGACATC 
AACAGTCAGC AGAACAGCAC CAACCGGACG AGCGAAACGC ACATGACCAT CCAGGGCCTT 
AATCCCGACA CCACCTACGA TATCCGGGTG CAGGCGTTCA CCAGTAAGGG CGGAGGACCC 
CTCAGCCCTA GCATCCAGAG CAGGACCATG TCGAACGATT TTCCCACCCT CAACTTTGGT 
GTCAAAGCCG TCATGAAAAC ATCTGTCCTC CTCACCTGGG ATTTGCCACC GAACTACAAA 
TCCCAAGTGC CTTTCAAGAT CCTGTACAAC CAGCAAAGCG TGGAAGTGCA GGGATCCCTG 
AAGAGGAGGC TGATCACGCA GCTGCAGCCA GACACGGACT ACTCTTTTGT GCTGACAAGC 
AGGGGCAACA TTGCTGGTGG TCTACAGCAG CAGGTGTCCA TCCGCACCGC TCCCGACCTG 
ATCAACGCCA AGCCCAGCAC ACATCAGCCT CAGGGTAGCA AGATGACAAT CAACCTGCCC 
AAGGTCCAGA CCACCACGCG CGTCAGGTGG TACTACATCG TGGTGGTGCC GGTGTCCCAG 
TCAACGCAGG GGTGGAAGAA TCCAGACGAG ATGGACCTGG ACGAGCTGTT CAAGTCAAGT 
GAAGGCCCCG TCCTGAGGCG CAGACGTCAC CTGGATTCCT CCAAACCTTA CATCGCTGCC 
AAGTTGGCCT CGTTGCCGAC CACCTTCACC CTGGGTGATG AGAAGAGATA CAACGGCTTC 
TACAACAAGC CCCTGAGCAG TCCGCAGGAG TATCTCTGCT TTGTCCTGGC AGTACTCCGA 
TCCGAAGGGG CAGACAGCAC TACTAACTAT ATGACATTTT CAGCCAGTCC CTACTCAGAC 
CCGGTCCACG TGCGAAACAG CATGGCTCAG GGGATGGAGC AGCCTGAAAT GTTGTGGGTT 
ATGGGCCCTG TTTTGGCCGT GGTCCTGATT ATCATCATCG TCATCGCCAT CCTGCTCTTC 
AAGAACAAAC AGGAAAGGAA GCGAGCGTCG CCCCTGCCCA AAGATGAGCA CTCGGGCGGG 
GTGAAGGACT CCCTGCTGGC CAACTCCTCC GACCCTGTGG AGATGAGAAG ACTTAACTAC 
CAGACCCCAG GTCCCAGCTC TCATCGCTGC CCCAACACTC CAAGAATGAG GGAGCACCCG 
CCCATCCCTG TCGTCGACCT GGCTGACCAC ATAGAGCGTC TGAAGGCCAA CGACGGCCTA 
CGCTTTTCCC AGGAGTACGA GTCGATTGAT CCAGGGCAGC AGTTTACCTG GGAGAACTCC 
AACATGGAAG TCAACAAGCC AAAGAATCGC TATGCAAACG TCATTGCCTA TGACCACTCC 
AGAGTGGTTC TCACCTCTGT TGACGCCGTT CCAGGCAGTG ACTACATCAA CGCCAACTAC 
ATCGACGGCT ACAGGAAGCA GAACGCCTAC ATCGCCACGC AGGGGCCACT TCCAGAGACA 
CTGAGCGACT TCTGGAGGAT GGTGTGGGAG CAAAGATCCA ACACCATCGT CATGATGACC 
AGACTGGAGG AGAAGTCACG GGTGAAGTGT GACCAGTACT GGCCGTCGCG AGGAACGGAG 
ACCTATGGGA TGATCCAGGT CACCATGCTG GACACCGTGG AGTTGGCCAC TTACAGCGTC 
CGTACCTTTG CCCTCTATAA GAATGGCTCC AGTGAGAAGA GAGAGGTGAG ACAGTTCCAG 
TTCATGGCCT GGCCAGACCA CGGGGTGCCA GAGTACCCCA CCCCAATCCT GGCCTTCCTA 
CGGCGGGTAA AGGCCTGCAA TCCTCCAGAC GCAGGGCCTA TGGTGGTCCA CTGCAGCGCG 
GGTGTGGGCA GGACCGGATG CTTCATCGTG ATAGATGCCA TGCTGGAGCG CATGAAGCAC 
GAGAAGTCCG TAGACATCTA CGGCCACGTG ACGTGCATGC GCGCCCAGAG GAACTACATG 
GTCCAGACCG AGGACCAGTA CATCTTCATT CACGAAGCCC TGCTGGAGGC CGCCACGTGC 
GGCAACACCG AGGTGCCGGC GCGCAACCTG TACGCCCACA TCCAGAAACT CACCCAGCCC 
CCGCCCGGCG AGACGGTTAC TGCCATGGAG CTGGAATTCA AGAGACTGGC CAACTCCAAA 
GCCCACACAT CCCGCTTCAT CAGCGCCAAC TTGCCCTGCA ACAAGTTCAA GAACCGCCTG 
GTCAACATCA TGCCTTTCGA GTCCACCCGT GTCTGCCTGC AGCCCATCCG TGGCGTCGAG 
GGCTCCGACT ACATCAATGC CAGCTGCATC GACGGCTACA GACAGCAGAA GGCCTACCTG 
GCCACGCAGG GCCCACTGGC AGAGACCACC GAGGACTTCT GGAGGATGCT GTGGGAGCAC 
AACTCCACCA TCGTCGTCAT GCTCACCAAG CTACGAGAGA TGGGCCGGGA GAAGTGCCAT 
CAGTACTGGC CAGCAGAGCG TTCAGCCAGG TACCAGTACT TTGTGGTGGA TCCAATGGCT 
GAGTACAACA TGCCCCAGTA CATCCTCAGA GAGTTCAAAG TCACAGATGC CAGGGACGGC 
CAGTCCAGAA CAATCCGCCA ATTCCAGTTC ACTGACTGGC CCGAGCAAGG AGTGCCAAAA 
ACCGGAGAGG GCTTCATTGA TTTTATCGGA CAAGTGCACA AAACCAAGGA ACAGTTCGGA 
CAGGACGGAC CAATCACTGT ACACTGCAGT GCTGGGGTGG GCAGGACCGG AGTCTTCATC 
ACCCTCAGCA TCGTGCTGGA GAGGATGAGG TATGAAGGAG TTGTCGACCT CTTCCAGACA 
GTGAAGACAC TTCGCACCCA GCGGCCTGCC ATGGTGCAGA CCGAGGACCA GTACCAGTTG 
TGCTACAGGG CAGCATTGGA GTACCTGGGG AGCTTTGACC ACTATGCAAC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010744746.2
CDS	523..6276
Translation	MVPNIHLAVPFLPRGALLFLILLSCVVFSSLADSMPSFVKSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVEKTRTATMLCAASGNPDPEISWFKDMLPVDISSSNGRIKQLRSGALQIENSEESDQGKYECVAMNSAGTRYSAPANLYVRVRRVPPRFSIPPTNQEVMPGGSVNLTCVAVGSPMPYVKWTMGLVELTKEEEMPMGRNVLEVNNIRESANYTCVAISSLGMIEATAQVTVKALPKPPASLIVTETTATSVTLTWDSGNPEPVSYYMIQYRAKTSDNGFQEVEGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSDPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRVYYSSDLTAPLSAWQKHNTDDSSLTTISGLTPDITYSLRVLGFTSVGDGPPSDILQVKTQQGVPAQPSSFEAEAELDSRIMLTWLWPVQDPITKYELQYWEAGSDNKLHVSFNPAGSYAVDGLKPDTLYRFSLAARSEMGLGVYTQPIEARTAQSTPSAPPQEVHLVSLSSTSLKVSWVAPPAASRHGAIVRYTVSYQAMAGEDTERHEVTGIGADATSFVLEGLEKWTEYQVWVRAHTDVGPGPESAPVRMRTKEDVPGAPPRKLEVEAINSTAIRVTWKPPLQGKQHGQIRGYQVIFSRLENGEPRGQPNIMDVALPEAQWNIEESTEHEAIIAGLHSETTYSVTVAAYTTKGDGARSKAKVITTTGAVPGKPTMMISTTMGNTALIQWQPPKEMVGELMGYQLQYKRTDEENYTSRELRKIDDHYTVTGLHKGATYVFRLSARNRAGIGEAYVKEISTPEDVPSGFPFNLQVTGLTQSSTELTWDPPLVAERNGKIVHYVVVYRDINSQQNSTNRTSETHMTIQGLNPDTTYDIRVQAFTSKGGGPLSPSIQSRTMSNDFPTLNFGVKAVMKTSVLLTWDLPPNYKSQVPFKILYNQQSVEVQGSLKRRLITQLQPDTDYSFVLTSRGNIAGGLQQQVSIRTAPDLINAKPSTHQPQGSKMTINLPKVQTTTRVRWYYIVVVPVSQSTQGWKNPDEMDLDELFKSSEGPVLRRRRHLDSSKPYIAAKLASLPTTFTLGDEKRYNGFYNKPLSSPQEYLCFVLAVLRSEGADSTTNYMTFSASPYSDPVHVRNSMAQGMEQPEMLWVMGPVLAVVLIIIIVIAILLFKNKQERKRASPLPKDEHSGGVKDSLLANSSDPVEMRRLNYQTPGPSSHRCPNTPRMREHPPIPVVDLADHIERLKANDGLRFSQEYESIDPGQQFTWENSNMEVNKPKNRYANVIAYDHSRVVLTSVDAVPGSDYINANYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRSNTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVTMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRAQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPPPGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYLATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_010746444.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010746444.2

ORF Insert Sequence:

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTTGACATC 
AGCAGCAGCA ACGGACGCAT TAAACAGCTT CGTTCAGGAG CTCTGCAAAT TGAGAACAGC 
GAGGAGTCCG ACCAAGGGAA ATACGAGTGT GTTGCCATGA ACAGCGCTGG GACCCGTTAC 
TCCGCTCCTG CCAATCTCTA CGTACGAGTG CGAAGAGTCC CACCCAGATT CTCCATTCCG 
CCCACCAACC AAGAGGTGAT GCCAGGCGGC AGCGTCAACC TGACATGCGT GGCAGTGGGC 
TCGCCGATGC CCTACGTCAA GTGGACGATG GGCCTGGTGG AGCTGACCAA GGAGGAGGAG 
ATGCCGATGG GACGCAACGT GCTGGAGGTC AACAACATCC GCGAGTCGGC GAACTACACC 
TGCGTGGCCA TCTCCTCTCT GGGCATGATA GAAGCCACCG CTCAGGTCAC CGTCAAAGCT 
TTGCCAAAGC CTCCCGCCTC CCTCATTGTG ACTGAGACCA CAGCCACCAG TGTGACTCTC 
ACCTGGGACT CTGGAAACCC AGAACCTGTC TCCTACTACA TGATCCAATA CAGAGCCAAG 
ACCTCGGACA ACGGCTTCCA GGAAGTGGAA GGTGTGGCAA CTACCCGTTA CAGCATCGGT 
GGACTTAGCC CCTACTCTGA GTATGAGTTT CGTGTCATGG CTGTCAACAA CATCGGCCGG 
GGGCCACCGA GCGACCCCGT GGAGACACGC ACTGGTGAAC AGGCCCCCTC CTCGCCACCT 
CTGCACGTCC AAGCGCGCAT GCTGAGTGCC AGCACAATGC TGGTCCAGTG GGAACCGCCC 
GAGGAACCCA ACGGGCAAAT CAGGGGCTAC CGGGTCTACT ACAGCTCGGA CTTGACGGCT 
CCGCTCAGCG CCTGGCAGAA GCACAACACA GACGACAGCA GCCTGACGAC CATCTCGGGC 
CTGACTCCTG ATATCACCTA CAGCCTCAGG GTGCTGGGCT TCACCTCTGT AGGTGACGGG 
CCACCTTCTG ACATCCTGCA AGTCAAAACC CAACAGGGAG TCCCTGCCCA GCCGTCCAGC 
TTTGAGGCCG AAGCTGAGCT GGACAGTCGC ATCATGCTAA CATGGCTGTG GCCCGTCCAG 
GACCCCATCA CTAAGTATGA GCTGCAGTAC TGGGAGGCCG GCTCAGACAA CAAGTTACAT 
GTGAGCTTCA ACCCAGCGGG CTCCTACGCT GTGGACGGTC TAAAGCCCGA CACCTTGTAC 
CGTTTCTCTC TGGCAGCCCG TTCTGAGATG GGCTTAGGTG TCTACACCCA ACCCATCGAG 
GCTCGCACCG CCCAGTCCAC CCCATCAGCT CCCCCCCAGG AGGTACACCT GGTCAGCCTG 
AGCTCCACCA GCCTCAAGGT GAGTTGGGTG GCACCGCCCG CCGCTAGCCG CCACGGCGCC 
ATTGTGCGCT ACACAGTCAG CTACCAGGCC ATGGCTGGAG AAGACACGGA GCGGCACGAG 
GTGACGGGCA TCGGCGCAGA CGCCACTAGC TTTGTGCTGG AGGGCCTGGA GAAGTGGACT 
GAGTATCAGG TGTGGGTGAG GGCACACACT GACGTGGGCC CCGGCCCAGA GAGTGCCCCA 
GTGAGGATGA GAACCAAAGA GGATGTGCCG GGAGCACCCC CCAGGAAGCT GGAGGTTGAG 
GCCATAAACT CCACAGCAAT CCGGGTCACC TGGAAGCCGC CCCTGCAGGG GAAGCAGCAC 
GGCCAAATCC GGGGCTATCA AGTCATCTTC TCTCGGCTTG AAAACGGCGA ACCACGAGGC 
CAGCCTAACA TCATGGACGT TGCTTTGCCA GAGGCACAGT GGAATATTGA GGAGTCCACC 
GAGCATGAGG CCATCATTGC CGGACTGCAC TCTGAAACCA CCTACTCGGT CACCGTGGCA 
GCATACACCA CCAAAGGAGA CGGAGCGCGC AGCAAAGCCA AAGTCATCAC CACGACGGGT 
GCAGTGCCAG GAAAGCCCAC CATGATGATC AGCACCACTA TGGGCAACAC AGCGCTGATT 
CAGTGGCAGC CACCTAAGGA AATGGTTGGG GAGCTGATGG GCTACCAGCT GCAGTACAAG 
CGCACTGACG AGGAAAACTA CACCTCCCGC GAGCTGAGAA AGATCGATGA CCACTACACC 
GTCACCGGGC TGCACAAAGG TGCCACCTAT GTCTTCCGCC TCAGTGCCCG CAACCGCGCG 
GGCATCGGCG AAGCATACGT GAAAGAGATC AGCACCCCTG AAGACGTGCC GTCGGGCTTC 
CCGTTCAACC TGCAGGTCAC GGGACTGACT CAGTCCAGCA CCGAGCTGAC CTGGGACCCT 
CCGCTGGTGG CCGAAAGGAA CGGCAAGATC GTGCACTATG TGGTCGTTTA CAGGGACATC 
AACAGTCAGC AGAACAGCAC CAACCGGACG AGCGAAACGC ACATGACCAT CCAGGGCCTT 
AATCCCGACA CCACCTACGA TATCCGGGTG CAGGCGTTCA CCAGTAAGGG CGGAGGACCC 
CTCAGCCCTA GCATCCAGAG CAGGACCATG TCGAACGATT TTCCCACCCT CAACTTTGGT 
GTCAAAGCCG TCATGAAAAC ATCTGTCCTC CTCACCTGGG ATTTGCCACC GAACTACAAA 
TCCCAAGTGC CTTTCAAGAT CCTGTACAAC CAGCAAAGCG TGGAAGTGCA GGGATCCCTG 
AAGAGGAGGC TGATCACGCA GCTGCAGCCA GACACGGACT ACTCTTTTGT GCTGACAAGC 
AGGGGCAACA TTGCTGGTGG TCTACAGCAG CAGGTGTCCA TCCGCACCGC TCCCGACCTG 
ATCAACGCCA AGCCCAGCAC ACATCAGCCT CAGGGTAGCA AGATGACAAT CAACCTGCCC 
AAGGTCCAGA CCACCACGCG CGTCAGGTGG TACTACATCG TGGTGGTGCC GGTGTCCCAG 
TCAACGCAGG GGTGGAAGAA TCCAGACGAG ATGGACCTGG ACGAGCTGTT CAAGTCAAGT 
GAAGGCCCCG TCCTGAGGCG CAGACGTCAC CTGGATTCCT CCAAACCTTA CATCGCTGCC 
AAGTTGGCCT CGTTGCCGAC CACCTTCACC CTGGGTGATG AGAAGAGATA CAACGGCTTC 
TACAACAAGC CCCTGAGCAG TCCGCAGGAG TATCTCTGCT TTGTCCTGGC AGTACTCCGA 
TCCGAAGGGG CAGACAGCAC TACTAACTAT ATGACATTTT CAGCCAGTCC CTACTCAGAC 
CCGGTCCACG TGCGAAACAG CATGGCTCAG GGGATGGAGC AGCCTGAAAT GTTGTGGGTT 
ATGGGCCCTG TTTTGGCCGT GGTCCTGATT ATCATCATCG TCATCGCCAT CCTGCTCTTC 
AAGAACAAAC AGGAAAGGAA GCGAGCGTCG CCCCTGCCCA AAGATGAGCA CTCGGGCGGG 
GTGAAGGACT CCCTGCTGGC CAACTCCTCC GACCCTGTGG AGATGAGAAG ACTTAACTAC 
CAGACCCCAG GTCCCAGCTC TCATCGCTGC CCCAACACTC CAAGAATGAG GGAGCACCCG 
CCCATCCCTG TCGTCGACCT GGCTGACCAC ATAGAGCGTC TGAAGGCCAA CGACGGCCTA 
CGCTTTTCCC AGGAGTACGA GTCGATTGAT CCAGGGCAGC AGTTTACCTG GGAGAACTCC 
AACATGGAAG TCAACAAGCC AAAGAATCGC TATGCAAACG TCATTGCCTA TGACCACTCC 
AGAGTGGTTC TCACCTCTGT TGACGCCGTT CCAGGCAGTG ACTACATCAA CGCCAACTAC 
ATCGACGGCT ACAGGAAGCA GAACGCCTAC ATCGCCACGC AGGGGCCACT TCCAGAGACA 
CTGAGCGACT TCTGGAGGAT GGTGTGGGAG CAAAGATCCA ACACCATCGT CATGATGACC 
AGACTGGAGG AGAAGTCACG GGTGAAGTGT GACCAGTACT GGCCGTCGCG AGGAACGGAG 
ACCTATGGGA TGATCCAGGT CACCATGCTG GACACCGTGG AGTTGGCCAC TTACAGCGTC 
CGTACCTTTG CCCTCTATAA GAATGGCTCC AGTGAGAAGA GAGAGGTGAG ACAGTTCCAG 
TTCATGGCCT GGCCAGACCA CGGGGTGCCA GAGTACCCCA CCCCAATCCT GGCCTTCCTA 
CGGCGGGTAA AGGCCTGCAA TCCTCCAGAC GCAGGGCCTA TGGTGGTCCA CTGCAGCGCG 
GGTGTGGGCA GGACCGGATG CTTCATCGTG ATAGATGCCA TGCTGGAGCG CATGAAGCAC 
GAGAAGTCCG TAGACATCTA CGGCCACGTG ACGTGCATGC GCGCCCAGAG GAACTACATG 
GTCCAGACCG AGGACCAGTA CATCTTCATT CACGAAGCCC TGCTGGAGGC CGCCACGTGC 
GGCAACACCG AGGTGCCGGC GCGCAACCTG TACGCCCACA TCCAGAAACT CACCCAGCCC 
CCGCCCGGCG AGACGGTTAC TGCCATGGAG CTGGAATTCA AGAGACTGGC CAACTCCAAA 
GCCCACACAT CCCGCTTCAT CAGCGCCAAC TTGCCCTGCA ACAAGTTCAA GAACCGCCTG 
GTCAACATCA TGCCTTTCGA GTCCACCCGT GTCTGCCTGC AGCCCATCCG TGGCGTCGAG 
GGCTCCGACT ACATCAATGC CAGCTGCATC GACGGCTACA GACAGCAGAA GGCCTACCTG 
GCCACGCAGG GCCCACTGGC AGAGACCACC GAGGACTTCT GGAGGATGCT GTGGGAGCAC 
AACTCCACCA TCGTCGTCAT GCTCACCAAG CTACGAGAGA TGGGCCGGGA GAAGTGCCAT 
CAGTACTGGC CAGCAGAGCG TTCAGCCAGG TACCAGTACT TTGTGGTGGA TCCAATGGCT 
GAGTACAACA TGCCCCAGTA CATCCTCAGA GAGTTCAAAG TCACAGATGC CAGGGACGGC 
CAGTCCAGAA CAATCCGCCA ATTCCAGTTC ACTGACTGGC CCGAGCAAGG AGTGCCAAAA 
ACCGGAGAGG GCTTCATTGA TTTTATCGGA CAAGTGCACA AAACCAAGGA ACAGTTCGGA 
CAGGACGGAC CAATCACTGT ACACTGCAGT GCTGGGGTGG GCAGGACCGG AGTCTTCATC 
ACCCTCAGCA TCGTGCTGGA GAGGATGAGG TATGAAGGAG TTGTCGACCT CTTCCAGACA 
GTGAAGACAC TTCGCACCCA GCGGCCTGCC ATGGTGCAGA CCGAGGACCA GTACCAGTTG 
TGCTACAGGG CAGCATTGGA GTACCTGGGG AGCTTTGACC ACTATGCAAC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104931439 ( XM_019263539.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019119084.1 Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X5, mRNA.

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CloneID	OLa84599
Clone ID Related Accession (Same CDS sequence)	XM_019263539.1
Accession Version	XM_019263539.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	receptor-type tyrosine-protein phosphatase F isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017607936.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTTGACATC 
AGCAGCAGCA ACGGACGCAT TAAACAGCTT CGTTCAGGTG GTACACCAAT TAGAGGAGCT 
CTGCAAATTG AGAACAGCGA GGAGTCCGAC CAAGGGAAAT ACGAGTGTGT TGCCATGAAC 
AGCGCTGGGA CCCGTTACTC CGCTCCTGCC AATCTCTACG TACGAGTGCG AAGAGTCCCA 
CCCAGATTCT CCATTCCGCC CACCAACCAA GAGGTGATGC CAGGCGGCAG CGTCAACCTG 
ACATGCGTGG CAGTGGGCTC GCCGATGCCC TACGTCAAGT GGACGATGGG CCTGGTGGAG 
CTGACCAAGG AGGAGGAGAT GCCGATGGGA CGCAACGTGC TGGAGGTCAA CAACATCCGC 
GAGTCGGCGA ACTACACCTG CGTGGCCATC TCCTCTCTGG GCATGATAGA AGCCACCGCT 
CAGGTCACCG TCAAAGCTTT GCCAAAGCCT CCCGCCTCCC TCATTGTGAC TGAGACCACA 
GCCACCAGTG TGACTCTCAC CTGGGACTCT GGAAACCCAG AACCTGTCTC CTACTACATG 
ATCCAATACA GAGCCAAGAC CTCGGACAAC GGCTTCCAGG AAGTGGAAGG TGTGGCAACT 
ACCCGTTACA GCATCGGTGG ACTTAGCCCC TACTCTGAGT ATGAGTTTCG TGTCATGGCT 
GTCAACAACA TCGGCCGGGG GCCACCGAGC GACCCCGTGG AGACACGCAC TGGTGAACAG 
GCCCCCTCCT CGCCACCTCT GCACGTCCAA GCGCGCATGC TGAGTGCCAG CACAATGCTG 
GTCCAGTGGG AACCGCCCGA GGAACCCAAC GGGCAAATCA GGGGCTACCG GGTCTACTAC 
AGCTCGGACT TGACGGCTCC GCTCAGCGCC TGGCAGAAGC ACAACACAGA CGACAGCAGC 
CTGACGACCA TCTCGGGCCT GACTCCTGAT ATCACCTACA GCCTCAGGGT GCTGGGCTTC 
ACCTCTGTAG GTGACGGGCC ACCTTCTGAC ATCCTGCAAG TCAAAACCCA ACAGGGAGTC 
CCTGCCCAGC CGTCCAGCTT TGAGGCCGAA GCTGAGCTGG ACAGTCGCAT CATGCTAACA 
TGGCTGTGGC CCGTCCAGGA CCCCATCACT AAGTATGAGC TGCAGTACTG GGAGGCCGGC 
TCAGACAACA AGTTACATGT GAGCTTCAAC CCAGCGGGCT CCTACGCTGT GGACGGTCTA 
AAGCCCGACA CCTTGTACCG TTTCTCTCTG GCAGCCCGTT CTGAGATGGG CTTAGGTGTC 
TACACCCAAC CCATCGAGGC TCGCACCGCC CAGTCCACCC CATCAGCTCC CCCCCAGGAG 
GTACACCTGG TCAGCCTGAG CTCCACCAGC CTCAAGGTGA GTTGGGTGGC ACCGCCCGCC 
GCTAGCCGCC ACGGCGCCAT TGTGCGCTAC ACAGTCAGCT ACCAGGCCAT GGCTGGAGAA 
GACACGGAGC GGCACGAGGT GACGGGCATC GGCGCAGACG CCACTAGCTT TGTGCTGGAG 
GGCCTGGAGA AGTGGACTGA GTATCAGGTG TGGGTGAGGG CACACACTGA CGTGGGCCCC 
GGCCCAGAGA GTGCCCCAGT GAGGATGAGA ACCAAAGAGG ATGTGCCGGG AGCACCCCCC 
AGGAAGCTGG AGGTTGAGGC CATAAACTCC ACAGCAATCC GGGTCACCTG GAAGCCGCCC 
CTGCAGGGGA AGCAGCACGG CCAAATCCGG GGCTATCAAG TCATCTTCTC TCGGCTTGAA 
AACGGCGAAC CACGAGGCCA GCCTAACATC ATGGACGTTG CTTTGCCAGA GGCACAGGAG 
GCCATCATTG CCGGACTGCA CTCTGAAACC ACCTACTCGG TCACCGTGGC AGCATACACC 
ACCAAAGGAG ACGGAGCGCG CAGCAAAGCC AAAGTCATCA CCACGACGGG TGCAGTGCCA 
GGAAAGCCCA CCATGATGAT CAGCACCACT ATGGGCAACA CAGCGCTGAT TCAGTGGCAG 
CCACCTAAGG AAATGGTTGG GGAGCTGATG GGCTACCAGC TGCAGTACAA GCGCACTGAC 
GAGGAAAACT ACACCTCCCG CGAGCTGAGA AAGATCGATG ACCACTACAC CGTCACCGGG 
CTGCACAAAG GTGCCACCTA TGTCTTCCGC CTCAGTGCCC GCAACCGCGC GGGCATCGGC 
GAAGCATACG TGAAAGAGAT CAGCACCCCT GAAGACGTGC CGTCGGGCTT CCCGTTCAAC 
CTGCAGGTCA CGGGACTGAC TCAGTCCAGC ACCGAGCTGA CCTGGGACCC TCCGCTGGTG 
GCCGAAAGGA ACGGCAAGAT CGTGCACTAT GTGGTCGTTT ACAGGGACAT CAACAGTCAG 
CAGAACAGCA CCAACCGGAC GAGCGAAACG CACATGACCA TCCAGGGCCT TAATCCCGAC 
ACCACCTACG ATATCCGGGT GCAGGCGTTC ACCAGTAAGG GCGGAGGACC CCTCAGCCCT 
AGCATCCAGA GCAGGACCAT GTCGAACGAT TTTCCCACCC TCAACTTTGG TGTCAAAGCC 
GTCATGAAAA CATCTGTCCT CCTCACCTGG GATTTGCCAC CGAACTACAA ATCCCAAGTG 
CCTTTCAAGA TCCTGTACAA CCAGCAAAGC GTGGAAGTGC AGGGATCCCT GAAGAGGAGG 
CTGATCACGC AGCTGCAGCC AGACACGGAC TACTCTTTTG TGCTGACAAG CAGGGGCAAC 
ATTGCTGGTG GTCTACAGCA GCAGGTGTCC ATCCGCACCG CTCCCGACCT GATCAACGCC 
AAGCCCAGCA CACATCAGCC TCAGGGTAGC AAGATGACAA TCAACCTGCC CAAGGTCCAG 
ACCACCACGC GCGTCAGGTG GTACTACATC GTGGTGGTGC CGGTGTCCCA GTCAACGCAG 
GGGTGGAAGA ATCCAGACGA GATGGACCTG GACGAGCTGT TCAAGTCAAG TGAAGGCCCC 
GTCCTGAGGC GCAGACGTCA CCTGGATTCC TCCAAACCTT ACATCGCTGC CAAGTTGGCC 
TCGTTGCCGA CCACCTTCAC CCTGGGTGAT GAGAAGAGAT ACAACGGCTT CTACAACAAG 
CCCCTGAGCA GTCCGCAGGA GTATCTCTGC TTTGTCCTGG CAGTACTCCG ATCCGAAGGG 
GCAGACAGCA CTACTAACTA TATGACATTT TCAGCCAGTC CCTACTCAGA CCCGGTCCAC 
GTGCGAAACA GCATGGCTCA GGGGATGGAG CAGCCTGAAA TGTTGTGGGT TATGGGCCCT 
GTTTTGGCCG TGGTCCTGAT TATCATCATC GTCATCGCCA TCCTGCTCTT CAAGAACAAA 
CAGGAAAGGA AGCGAGCGTC GCCCCTGCCC AAAGATGAGC ACTCGGGCGG GGTGAAGGAC 
TCCCTGCTGG CCAACTCCTC CGACCCTGTG GAGATGAGAA GACTTAACTA CCAGACCCCA 
GGTCCCAGCT CTCATCGCTG CCCCAACACT CCAAGAATGA GGGAGCACCC GCCCATCCCT 
GTCGTCGACC TGGCTGACCA CATAGAGCGT CTGAAGGCCA ACGACGGCCT ACGCTTTTCC 
CAGGAGTACG AGTCGATTGA TCCAGGGCAG CAGTTTACCT GGGAGAACTC CAACATGGAA 
GTCAACAAGC CAAAGAATCG CTATGCAAAC GTCATTGCCT ATGACCACTC CAGAGTGGTT 
CTCACCTCTG TTGACGCCGT TCCAGGCAGT GACTACATCA ACGCCAACTA CATCGACGGC 
TACAGGAAGC AGAACGCCTA CATCGCCACG CAGGGGCCAC TTCCAGAGAC ACTGAGCGAC 
TTCTGGAGGA TGGTGTGGGA GCAAAGATCC AACACCATCG TCATGATGAC CAGACTGGAG 
GAGAAGTCAC GGGTGAAGTG TGACCAGTAC TGGCCGTCGC GAGGAACGGA GACCTATGGG 
ATGATCCAGG TCACCATGCT GGACACCGTG GAGTTGGCCA CTTACAGCGT CCGTACCTTT 
GCCCTCTATA AGAATGGCTC CAGTGAGAAG AGAGAGGTGA GACAGTTCCA GTTCATGGCC 
TGGCCAGACC ACGGGGTGCC AGAGTACCCC ACCCCAATCC TGGCCTTCCT ACGGCGGGTA 
AAGGCCTGCA ATCCTCCAGA CGCAGGGCCT ATGGTGGTCC ACTGCAGCGC GGGTGTGGGC 
AGGACCGGAT GCTTCATCGT GATAGATGCC ATGCTGGAGC GCATGAAGCA CGAGAAGTCC 
GTAGACATCT ACGGCCACGT GACGTGCATG CGCGCCCAGA GGAACTACAT GGTCCAGACC 
GAGGACCAGT ACATCTTCAT TCACGAAGCC CTGCTGGAGG CCGCCACGTG CGGCAACACC 
GAGGTGCCGG CGCGCAACCT GTACGCCCAC ATCCAGAAAC TCACCCAGCC CCCGCCCGGC 
GAGACGGTTA CTGCCATGGA GCTGGAATTC AAGAGACTGG CCAACTCCAA AGCCCACACA 
TCCCGCTTCA TCAGCGCCAA CTTGCCCTGC AACAAGTTCA AGAACCGCCT GGTCAACATC 
ATGCCTTTCG AGTCCACCCG TGTCTGCCTG CAGCCCATCC GTGGCGTCGA GGGCTCCGAC 
TACATCAATG CCAGCTGCAT CGACGGCTAC AGACAGCAGA AGGCCTACCT GGCCACGCAG 
GGCCCACTGG CAGAGACCAC CGAGGACTTC TGGAGGATGC TGTGGGAGCA CAACTCCACC 
ATCGTCGTCA TGCTCACCAA GCTACGAGAG ATGGGCCGGG AGAAGTGCCA TCAGTACTGG 
CCAGCAGAGC GTTCAGCCAG GTACCAGTAC TTTGTGGTGG ATCCAATGGC TGAGTACAAC 
ATGCCCCAGT ACATCCTCAG AGAGTTCAAA GTCACAGATG CCAGGGACGG CCAGTCCAGA 
ACAATCCGCC AATTCCAGTT CACTGACTGG CCCGAGCAAG GAGTGCCAAA AACCGGAGAG 
GGCTTCATTG ATTTTATCGG ACAAGTGCAC AAAACCAAGG AACAGTTCGG ACAGGACGGA 
CCAATCACTG TACACTGCAG TGCTGGGGTG GGCAGGACCG GAGTCTTCAT CACCCTCAGC 
ATCGTGCTGG AGAGGATGAG GTATGAAGGA GTTGTCGACC TCTTCCAGAC AGTGAAGACA 
CTTCGCACCC AGCGGCCTGC CATGGTGCAG ACCGAGGACC AGTACCAGTT GTGCTACAGG 
GCAGCATTGG AGTACCTGGG GAGCTTTGAC CACTATGCAA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019119084.1
CDS	523..6267
Translation	MVPNIHLAVPFLPRGALLFLILLSCVVFSSLADSMPSFVKSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVEKTRTATMLCAASGNPDPEISWFKDMLPVDISSSNGRIKQLRSGGTPIRGALQIENSEESDQGKYECVAMNSAGTRYSAPANLYVRVRRVPPRFSIPPTNQEVMPGGSVNLTCVAVGSPMPYVKWTMGLVELTKEEEMPMGRNVLEVNNIRESANYTCVAISSLGMIEATAQVTVKALPKPPASLIVTETTATSVTLTWDSGNPEPVSYYMIQYRAKTSDNGFQEVEGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSDPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRVYYSSDLTAPLSAWQKHNTDDSSLTTISGLTPDITYSLRVLGFTSVGDGPPSDILQVKTQQGVPAQPSSFEAEAELDSRIMLTWLWPVQDPITKYELQYWEAGSDNKLHVSFNPAGSYAVDGLKPDTLYRFSLAARSEMGLGVYTQPIEARTAQSTPSAPPQEVHLVSLSSTSLKVSWVAPPAASRHGAIVRYTVSYQAMAGEDTERHEVTGIGADATSFVLEGLEKWTEYQVWVRAHTDVGPGPESAPVRMRTKEDVPGAPPRKLEVEAINSTAIRVTWKPPLQGKQHGQIRGYQVIFSRLENGEPRGQPNIMDVALPEAQEAIIAGLHSETTYSVTVAAYTTKGDGARSKAKVITTTGAVPGKPTMMISTTMGNTALIQWQPPKEMVGELMGYQLQYKRTDEENYTSRELRKIDDHYTVTGLHKGATYVFRLSARNRAGIGEAYVKEISTPEDVPSGFPFNLQVTGLTQSSTELTWDPPLVAERNGKIVHYVVVYRDINSQQNSTNRTSETHMTIQGLNPDTTYDIRVQAFTSKGGGPLSPSIQSRTMSNDFPTLNFGVKAVMKTSVLLTWDLPPNYKSQVPFKILYNQQSVEVQGSLKRRLITQLQPDTDYSFVLTSRGNIAGGLQQQVSIRTAPDLINAKPSTHQPQGSKMTINLPKVQTTTRVRWYYIVVVPVSQSTQGWKNPDEMDLDELFKSSEGPVLRRRRHLDSSKPYIAAKLASLPTTFTLGDEKRYNGFYNKPLSSPQEYLCFVLAVLRSEGADSTTNYMTFSASPYSDPVHVRNSMAQGMEQPEMLWVMGPVLAVVLIIIIVIAILLFKNKQERKRASPLPKDEHSGGVKDSLLANSSDPVEMRRLNYQTPGPSSHRCPNTPRMREHPPIPVVDLADHIERLKANDGLRFSQEYESIDPGQQFTWENSNMEVNKPKNRYANVIAYDHSRVVLTSVDAVPGSDYINANYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRSNTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVTMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRAQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPPPGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYLATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_019263539.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019263539.1

ORF Insert Sequence:

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTTGACATC 
AGCAGCAGCA ACGGACGCAT TAAACAGCTT CGTTCAGGTG GTACACCAAT TAGAGGAGCT 
CTGCAAATTG AGAACAGCGA GGAGTCCGAC CAAGGGAAAT ACGAGTGTGT TGCCATGAAC 
AGCGCTGGGA CCCGTTACTC CGCTCCTGCC AATCTCTACG TACGAGTGCG AAGAGTCCCA 
CCCAGATTCT CCATTCCGCC CACCAACCAA GAGGTGATGC CAGGCGGCAG CGTCAACCTG 
ACATGCGTGG CAGTGGGCTC GCCGATGCCC TACGTCAAGT GGACGATGGG CCTGGTGGAG 
CTGACCAAGG AGGAGGAGAT GCCGATGGGA CGCAACGTGC TGGAGGTCAA CAACATCCGC 
GAGTCGGCGA ACTACACCTG CGTGGCCATC TCCTCTCTGG GCATGATAGA AGCCACCGCT 
CAGGTCACCG TCAAAGCTTT GCCAAAGCCT CCCGCCTCCC TCATTGTGAC TGAGACCACA 
GCCACCAGTG TGACTCTCAC CTGGGACTCT GGAAACCCAG AACCTGTCTC CTACTACATG 
ATCCAATACA GAGCCAAGAC CTCGGACAAC GGCTTCCAGG AAGTGGAAGG TGTGGCAACT 
ACCCGTTACA GCATCGGTGG ACTTAGCCCC TACTCTGAGT ATGAGTTTCG TGTCATGGCT 
GTCAACAACA TCGGCCGGGG GCCACCGAGC GACCCCGTGG AGACACGCAC TGGTGAACAG 
GCCCCCTCCT CGCCACCTCT GCACGTCCAA GCGCGCATGC TGAGTGCCAG CACAATGCTG 
GTCCAGTGGG AACCGCCCGA GGAACCCAAC GGGCAAATCA GGGGCTACCG GGTCTACTAC 
AGCTCGGACT TGACGGCTCC GCTCAGCGCC TGGCAGAAGC ACAACACAGA CGACAGCAGC 
CTGACGACCA TCTCGGGCCT GACTCCTGAT ATCACCTACA GCCTCAGGGT GCTGGGCTTC 
ACCTCTGTAG GTGACGGGCC ACCTTCTGAC ATCCTGCAAG TCAAAACCCA ACAGGGAGTC 
CCTGCCCAGC CGTCCAGCTT TGAGGCCGAA GCTGAGCTGG ACAGTCGCAT CATGCTAACA 
TGGCTGTGGC CCGTCCAGGA CCCCATCACT AAGTATGAGC TGCAGTACTG GGAGGCCGGC 
TCAGACAACA AGTTACATGT GAGCTTCAAC CCAGCGGGCT CCTACGCTGT GGACGGTCTA 
AAGCCCGACA CCTTGTACCG TTTCTCTCTG GCAGCCCGTT CTGAGATGGG CTTAGGTGTC 
TACACCCAAC CCATCGAGGC TCGCACCGCC CAGTCCACCC CATCAGCTCC CCCCCAGGAG 
GTACACCTGG TCAGCCTGAG CTCCACCAGC CTCAAGGTGA GTTGGGTGGC ACCGCCCGCC 
GCTAGCCGCC ACGGCGCCAT TGTGCGCTAC ACAGTCAGCT ACCAGGCCAT GGCTGGAGAA 
GACACGGAGC GGCACGAGGT GACGGGCATC GGCGCAGACG CCACTAGCTT TGTGCTGGAG 
GGCCTGGAGA AGTGGACTGA GTATCAGGTG TGGGTGAGGG CACACACTGA CGTGGGCCCC 
GGCCCAGAGA GTGCCCCAGT GAGGATGAGA ACCAAAGAGG ATGTGCCGGG AGCACCCCCC 
AGGAAGCTGG AGGTTGAGGC CATAAACTCC ACAGCAATCC GGGTCACCTG GAAGCCGCCC 
CTGCAGGGGA AGCAGCACGG CCAAATCCGG GGCTATCAAG TCATCTTCTC TCGGCTTGAA 
AACGGCGAAC CACGAGGCCA GCCTAACATC ATGGACGTTG CTTTGCCAGA GGCACAGGAG 
GCCATCATTG CCGGACTGCA CTCTGAAACC ACCTACTCGG TCACCGTGGC AGCATACACC 
ACCAAAGGAG ACGGAGCGCG CAGCAAAGCC AAAGTCATCA CCACGACGGG TGCAGTGCCA 
GGAAAGCCCA CCATGATGAT CAGCACCACT ATGGGCAACA CAGCGCTGAT TCAGTGGCAG 
CCACCTAAGG AAATGGTTGG GGAGCTGATG GGCTACCAGC TGCAGTACAA GCGCACTGAC 
GAGGAAAACT ACACCTCCCG CGAGCTGAGA AAGATCGATG ACCACTACAC CGTCACCGGG 
CTGCACAAAG GTGCCACCTA TGTCTTCCGC CTCAGTGCCC GCAACCGCGC GGGCATCGGC 
GAAGCATACG TGAAAGAGAT CAGCACCCCT GAAGACGTGC CGTCGGGCTT CCCGTTCAAC 
CTGCAGGTCA CGGGACTGAC TCAGTCCAGC ACCGAGCTGA CCTGGGACCC TCCGCTGGTG 
GCCGAAAGGA ACGGCAAGAT CGTGCACTAT GTGGTCGTTT ACAGGGACAT CAACAGTCAG 
CAGAACAGCA CCAACCGGAC GAGCGAAACG CACATGACCA TCCAGGGCCT TAATCCCGAC 
ACCACCTACG ATATCCGGGT GCAGGCGTTC ACCAGTAAGG GCGGAGGACC CCTCAGCCCT 
AGCATCCAGA GCAGGACCAT GTCGAACGAT TTTCCCACCC TCAACTTTGG TGTCAAAGCC 
GTCATGAAAA CATCTGTCCT CCTCACCTGG GATTTGCCAC CGAACTACAA ATCCCAAGTG 
CCTTTCAAGA TCCTGTACAA CCAGCAAAGC GTGGAAGTGC AGGGATCCCT GAAGAGGAGG 
CTGATCACGC AGCTGCAGCC AGACACGGAC TACTCTTTTG TGCTGACAAG CAGGGGCAAC 
ATTGCTGGTG GTCTACAGCA GCAGGTGTCC ATCCGCACCG CTCCCGACCT GATCAACGCC 
AAGCCCAGCA CACATCAGCC TCAGGGTAGC AAGATGACAA TCAACCTGCC CAAGGTCCAG 
ACCACCACGC GCGTCAGGTG GTACTACATC GTGGTGGTGC CGGTGTCCCA GTCAACGCAG 
GGGTGGAAGA ATCCAGACGA GATGGACCTG GACGAGCTGT TCAAGTCAAG TGAAGGCCCC 
GTCCTGAGGC GCAGACGTCA CCTGGATTCC TCCAAACCTT ACATCGCTGC CAAGTTGGCC 
TCGTTGCCGA CCACCTTCAC CCTGGGTGAT GAGAAGAGAT ACAACGGCTT CTACAACAAG 
CCCCTGAGCA GTCCGCAGGA GTATCTCTGC TTTGTCCTGG CAGTACTCCG ATCCGAAGGG 
GCAGACAGCA CTACTAACTA TATGACATTT TCAGCCAGTC CCTACTCAGA CCCGGTCCAC 
GTGCGAAACA GCATGGCTCA GGGGATGGAG CAGCCTGAAA TGTTGTGGGT TATGGGCCCT 
GTTTTGGCCG TGGTCCTGAT TATCATCATC GTCATCGCCA TCCTGCTCTT CAAGAACAAA 
CAGGAAAGGA AGCGAGCGTC GCCCCTGCCC AAAGATGAGC ACTCGGGCGG GGTGAAGGAC 
TCCCTGCTGG CCAACTCCTC CGACCCTGTG GAGATGAGAA GACTTAACTA CCAGACCCCA 
GGTCCCAGCT CTCATCGCTG CCCCAACACT CCAAGAATGA GGGAGCACCC GCCCATCCCT 
GTCGTCGACC TGGCTGACCA CATAGAGCGT CTGAAGGCCA ACGACGGCCT ACGCTTTTCC 
CAGGAGTACG AGTCGATTGA TCCAGGGCAG CAGTTTACCT GGGAGAACTC CAACATGGAA 
GTCAACAAGC CAAAGAATCG CTATGCAAAC GTCATTGCCT ATGACCACTC CAGAGTGGTT 
CTCACCTCTG TTGACGCCGT TCCAGGCAGT GACTACATCA ACGCCAACTA CATCGACGGC 
TACAGGAAGC AGAACGCCTA CATCGCCACG CAGGGGCCAC TTCCAGAGAC ACTGAGCGAC 
TTCTGGAGGA TGGTGTGGGA GCAAAGATCC AACACCATCG TCATGATGAC CAGACTGGAG 
GAGAAGTCAC GGGTGAAGTG TGACCAGTAC TGGCCGTCGC GAGGAACGGA GACCTATGGG 
ATGATCCAGG TCACCATGCT GGACACCGTG GAGTTGGCCA CTTACAGCGT CCGTACCTTT 
GCCCTCTATA AGAATGGCTC CAGTGAGAAG AGAGAGGTGA GACAGTTCCA GTTCATGGCC 
TGGCCAGACC ACGGGGTGCC AGAGTACCCC ACCCCAATCC TGGCCTTCCT ACGGCGGGTA 
AAGGCCTGCA ATCCTCCAGA CGCAGGGCCT ATGGTGGTCC ACTGCAGCGC GGGTGTGGGC 
AGGACCGGAT GCTTCATCGT GATAGATGCC ATGCTGGAGC GCATGAAGCA CGAGAAGTCC 
GTAGACATCT ACGGCCACGT GACGTGCATG CGCGCCCAGA GGAACTACAT GGTCCAGACC 
GAGGACCAGT ACATCTTCAT TCACGAAGCC CTGCTGGAGG CCGCCACGTG CGGCAACACC 
GAGGTGCCGG CGCGCAACCT GTACGCCCAC ATCCAGAAAC TCACCCAGCC CCCGCCCGGC 
GAGACGGTTA CTGCCATGGA GCTGGAATTC AAGAGACTGG CCAACTCCAA AGCCCACACA 
TCCCGCTTCA TCAGCGCCAA CTTGCCCTGC AACAAGTTCA AGAACCGCCT GGTCAACATC 
ATGCCTTTCG AGTCCACCCG TGTCTGCCTG CAGCCCATCC GTGGCGTCGA GGGCTCCGAC 
TACATCAATG CCAGCTGCAT CGACGGCTAC AGACAGCAGA AGGCCTACCT GGCCACGCAG 
GGCCCACTGG CAGAGACCAC CGAGGACTTC TGGAGGATGC TGTGGGAGCA CAACTCCACC 
ATCGTCGTCA TGCTCACCAA GCTACGAGAG ATGGGCCGGG AGAAGTGCCA TCAGTACTGG 
CCAGCAGAGC GTTCAGCCAG GTACCAGTAC TTTGTGGTGG ATCCAATGGC TGAGTACAAC 
ATGCCCCAGT ACATCCTCAG AGAGTTCAAA GTCACAGATG CCAGGGACGG CCAGTCCAGA 
ACAATCCGCC AATTCCAGTT CACTGACTGG CCCGAGCAAG GAGTGCCAAA AACCGGAGAG 
GGCTTCATTG ATTTTATCGG ACAAGTGCAC AAAACCAAGG AACAGTTCGG ACAGGACGGA 
CCAATCACTG TACACTGCAG TGCTGGGGTG GGCAGGACCG GAGTCTTCAT CACCCTCAGC 
ATCGTGCTGG AGAGGATGAG GTATGAAGGA GTTGTCGACC TCTTCCAGAC AGTGAAGACA 
CTTCGCACCC AGCGGCCTGC CATGGTGCAG ACCGAGGACC AGTACCAGTT GTGCTACAGG 
GCAGCATTGG AGTACCTGGG GAGCTTTGAC CACTATGCAA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104931439 ( XM_019263543.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019119088.1 Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X6, mRNA.

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CloneID	OLa84600
Clone ID Related Accession (Same CDS sequence)	XM_019263543.1
Accession Version	XM_019263543.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	receptor-type tyrosine-protein phosphatase F isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017607936.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTTGACATC 
AGCAGCAGCA ACGGACGCAT TAAACAGCTT CGTTCAGGTG GTACACCAAT TAGAGGAGCT 
CTGCAAATTG AGAACAGCGA GGAGTCCGAC CAAGGGAAAT ACGAGTGTGT TGCCATGAAC 
AGCGCTGGGA CCCGTTACTC CGCTCCTGCC AATCTCTACG TACGAGTGCG AAGAGTCCCA 
CCCAGATTCT CCATTCCGCC CACCAACCAA GAGGTGATGC CAGGCGGCAG CGTCAACCTG 
ACATGCGTGG CAGTGGGCTC GCCGATGCCC TACGTCAAGT GGACGATGGG CCTGGTGGAG 
CTGACCAAGG AGGAGGAGAT GCCGATGGGA CGCAACGTGC TGGAGGTCAA CAACATCCGC 
GAGTCGGCGA ACTACACCTG CGTGGCCATC TCCTCTCTGG GCATGATAGA AGCCACCGCT 
CAGGTCACCG TCAAAGCTTT GCCAAAGCCT CCCGCCTCCC TCATTGTGAC TGAGACCACA 
GCCACCAGTG TGACTCTCAC CTGGGACTCT GGAAACCCAG AACCTGTCTC CTACTACATG 
ATCCAATACA GAGCCAAGAC CTCGGACAAC GGCTTCCAGG AAGTGGAAGG TGTGGCAACT 
ACCCGTTACA GCATCGGTGG ACTTAGCCCC TACTCTGAGT ATGAGTTTCG TGTCATGGCT 
GTCAACAACA TCGGCCGGGG GCCACCGAGC GACCCCGTGG AGACACGCAC TGGTGAACAG 
GCCCCCTCCT CGCCACCTCT GCACGTCCAA GCGCGCATGC TGAGTGCCAG CACAATGCTG 
GTCCAGTGGG AACCGCCCGA GGAACCCAAC GGGCAAATCA GGGGCTACCG GGTCTACTAC 
AGCTCGGACT TGACGGCTCC GCTCAGCGCC TGGCAGAAGC ACAACACAGA CGACAGCAGC 
CTGACGACCA TCTCGGGCCT GACTCCTGAT ATCACCTACA GCCTCAGGGT GCTGGGCTTC 
ACCTCTGTAG GTGACGGGCC ACCTTCTGAC ATCCTGCAAG TCAAAACCCA ACAGGGAGTC 
CCTGCCCAGC CGTCCAGCTT TGAGGCCGAA GCTGAGCTGG ACAGTCGCAT CATGCTAACA 
TGGCTGTGGC CCGTCCAGGA CCCCATCACT AAGTATGAGC TGCAGTACTG GGAGGCCGGC 
TCAGACAACA AGTTACATGT GAGCTTCAAC CCAGCGGGCT CCTACGCTGT GGACGGTCTA 
AAGCCCGACA CCTTGTACCG TTTCTCTCTG GCAGCCCGTT CTGAGATGGG CTTAGGTGTC 
TACACCCAAC CCATCGAGGC TCGCACCGCC CAGTCCACCC CATCAGCTCC CCCCCAGGAG 
GTACACCTGG TCAGCCTGAG CTCCACCAGC CTCAAGGTGA GTTGGGTGGC ACCGCCCGCC 
GCTAGCCGCC ACGGCGCCAT TGTGCGCTAC ACAGTCAGCT ACCAGGCCAT GGCTGGAGAA 
GACACGGAGC GGCACGAGGT GACGGGCATC GGCGCAGACG CCACTAGCTT TGTGCTGGAG 
GGCCTGGAGA AGTGGACTGA GTATCAGGTG TGGGTGAGGG CACACACTGA CGTGGGCCCC 
GGCCCAGAGA GTGCCCCAGT GAGGATGAGA ACCAAAGAGG ATGTGCCGGG AGCACCCCCC 
AGGAAGCTGG AGGTTGAGGC CATAAACTCC ACAGCAATCC GGGTCACCTG GAAGCCGCCC 
CTGCAGGGGA AGCAGCACGG CCAAATCCGG GGCTATCAAG TCATCTTCTC TCGGCTTGAA 
AACGGCGAAC CACGAGGCCA GCCTAACATC ATGGACGTTG CTTTGCCAGA GGCACAGTGG 
AATATTGAGG AGTCCACCGA GCATGAGGCC ATCATTGCCG GACTGCACTC TGAAACCACC 
TACTCGGTCA CCGTGGCAGC ATACACCACC AAAGGAGACG GAGCGCGCAG CAAAGCCAAA 
GTCATCACCA CGACGGGTGC AGTGCCAGGA AAGCCCACCA TGATGATCAG CACCACTATG 
GGCAACACAG CGCTGATTCA GTGGCAGCCA CCTAAGGAAA TGGTTGGGGA GCTGATGGGC 
TACCAGCTGC AGTACAAGCG CACTGACGAG GAAAACTACA CCTCCCGCGA GCTGAGAAAG 
ATCGATGACC ACTACACCGT CACCGGGCTG CACAAAGGTG CCACCTATGT CTTCCGCCTC 
AGTGCCCGCA ACCGCGCGGG CATCGGCGAA GCATACGTGA AAGAGATCAG CACCCCTGAA 
GACGTGCCGT CGGGCTTCCC GTTCAACCTG CAGGTCACGG GACTGACTCA GTCCAGCACC 
GAGCTGACCT GGGACCCTCC GCTGGTGGCC GAAAGGAACG GCAAGATCGT GCACTATGTG 
GTCGTTTACA GGGACATCAA CAGTCAGCAG AACAGCACCA ACCGGACGAG CGAAACGCAC 
ATGACCATCC AGGGCCTTAA TCCCGACACC ACCTACGATA TCCGGGTGCA GGCGTTCACC 
AGTAAGGGCG GAGGACCCCT CAGCCCTAGC ATCCAGAGCA GGACCATGTC GAACGATTTT 
CCCACCCTCA ACTTTGGTGT CAAAGCCGTC ATGAAAACAT CTGTCCTCCT CACCTGGGAT 
TTGCCACCGA ACTACAAATC CCAAGTGCCT TTCAAGATCC TGTACAACCA GCAAAGCGTG 
GAAGTGCAGG GATCCCTGAA GAGGAGGCTG ATCACGCAGC TGCAGCCAGA CACGGACTAC 
TCTTTTGTGC TGACAAGCAG GGGCAACATT GCTGGTGGTC TACAGCAGCA GGTGTCCATC 
CGCACCGCTC CCGACCTGAT CAACGCCAAG CCCAGCACAC ATCAGCCTCA GGGTAGCAAG 
ATGACAATCA ACCTGCCCAA GGTCCAGACC ACCACGCGCG TCAGGTGGTA CTACATCGTG 
GTGGTGCCGG TGTCCCAGTC AACGCAGGGG TGGAAGAATC CAGACGAGAT GGACCTGGAC 
GAGCTGTTCA AGTCAAGTGA AGGCCCCGTC CTGAGGCGCA GACGTCACCT GGATTCCTCC 
AAACCTTACA TCGCTGCCAA GTTGGCCTCG TTGCCGACCA CCTTCACCCT GGGTGATGAG 
AAGAGATACA ACGGCTTCTA CAACAAGCCC CTGAGCAGTC CGCAGGAGTA TCTCTGCTTT 
GTCCTGGCAG TACTCCGATC CGAAGGGGCA GACAGCACTA CTAACTATAT GACATTTTCA 
GCCAGTCCCT ACTCAGACCC GGTCCACGTG CGAAACAGCA TGGCTCAGGG GATGGAGCAG 
CCTGAAATGT TGTGGGTTAT GGGCCCTGTT TTGGCCGTGG TCCTGATTAT CATCATCGTC 
ATCGCCATCC TGCTCTTCAA GAACAAACAG GAAAGGAAGC GAGCGTCGCC CCTGCCCAAA 
GATGAGCACT CGGGCGGGGT GAAGGACTCC CTGCTGGCCA ACTCCTCCGA CCCTGTGGAG 
ATGAGAAGAC TTAACTACCA GACCCCAGGA ATGAGGGAGC ACCCGCCCAT CCCTGTCGTC 
GACCTGGCTG ACCACATAGA GCGTCTGAAG GCCAACGACG GCCTACGCTT TTCCCAGGAG 
TACGAGTCGA TTGATCCAGG GCAGCAGTTT ACCTGGGAGA ACTCCAACAT GGAAGTCAAC 
AAGCCAAAGA ATCGCTATGC AAACGTCATT GCCTATGACC ACTCCAGAGT GGTTCTCACC 
TCTGTTGACG CCGTTCCAGG CAGTGACTAC ATCAACGCCA ACTACATCGA CGGCTACAGG 
AAGCAGAACG CCTACATCGC CACGCAGGGG CCACTTCCAG AGACACTGAG CGACTTCTGG 
AGGATGGTGT GGGAGCAAAG ATCCAACACC ATCGTCATGA TGACCAGACT GGAGGAGAAG 
TCACGGGTGA AGTGTGACCA GTACTGGCCG TCGCGAGGAA CGGAGACCTA TGGGATGATC 
CAGGTCACCA TGCTGGACAC CGTGGAGTTG GCCACTTACA GCGTCCGTAC CTTTGCCCTC 
TATAAGAATG GCTCCAGTGA GAAGAGAGAG GTGAGACAGT TCCAGTTCAT GGCCTGGCCA 
GACCACGGGG TGCCAGAGTA CCCCACCCCA ATCCTGGCCT TCCTACGGCG GGTAAAGGCC 
TGCAATCCTC CAGACGCAGG GCCTATGGTG GTCCACTGCA GCGCGGGTGT GGGCAGGACC 
GGATGCTTCA TCGTGATAGA TGCCATGCTG GAGCGCATGA AGCACGAGAA GTCCGTAGAC 
ATCTACGGCC ACGTGACGTG CATGCGCGCC CAGAGGAACT ACATGGTCCA GACCGAGGAC 
CAGTACATCT TCATTCACGA AGCCCTGCTG GAGGCCGCCA CGTGCGGCAA CACCGAGGTG 
CCGGCGCGCA ACCTGTACGC CCACATCCAG AAACTCACCC AGCCCCCGCC CGGCGAGACG 
GTTACTGCCA TGGAGCTGGA ATTCAAGAGA CTGGCCAACT CCAAAGCCCA CACATCCCGC 
TTCATCAGCG CCAACTTGCC CTGCAACAAG TTCAAGAACC GCCTGGTCAA CATCATGCCT 
TTCGAGTCCA CCCGTGTCTG CCTGCAGCCC ATCCGTGGCG TCGAGGGCTC CGACTACATC 
AATGCCAGCT GCATCGACGG CTACAGACAG CAGAAGGCCT ACCTGGCCAC GCAGGGCCCA 
CTGGCAGAGA CCACCGAGGA CTTCTGGAGG ATGCTGTGGG AGCACAACTC CACCATCGTC 
GTCATGCTCA CCAAGCTACG AGAGATGGGC CGGGAGAAGT GCCATCAGTA CTGGCCAGCA 
GAGCGTTCAG CCAGGTACCA GTACTTTGTG GTGGATCCAA TGGCTGAGTA CAACATGCCC 
CAGTACATCC TCAGAGAGTT CAAAGTCACA GATGCCAGGG ACGGCCAGTC CAGAACAATC 
CGCCAATTCC AGTTCACTGA CTGGCCCGAG CAAGGAGTGC CAAAAACCGG AGAGGGCTTC 
ATTGATTTTA TCGGACAAGT GCACAAAACC AAGGAACAGT TCGGACAGGA CGGACCAATC 
ACTGTACACT GCAGTGCTGG GGTGGGCAGG ACCGGAGTCT TCATCACCCT CAGCATCGTG 
CTGGAGAGGA TGAGGTATGA AGGAGTTGTC GACCTCTTCC AGACAGTGAA GACACTTCGC 
ACCCAGCGGC CTGCCATGGT GCAGACCGAG GACCAGTACC AGTTGTGCTA CAGGGCAGCA 
TTGGAGTACC TGGGGAGCTT TGACCACTAT GCAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019119088.1
CDS	522..6260
Translation	MVPNIHLAVPFLPRGALLFLILLSCVVFSSLADSMPSFVKSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVEKTRTATMLCAASGNPDPEISWFKDMLPVDISSSNGRIKQLRSGGTPIRGALQIENSEESDQGKYECVAMNSAGTRYSAPANLYVRVRRVPPRFSIPPTNQEVMPGGSVNLTCVAVGSPMPYVKWTMGLVELTKEEEMPMGRNVLEVNNIRESANYTCVAISSLGMIEATAQVTVKALPKPPASLIVTETTATSVTLTWDSGNPEPVSYYMIQYRAKTSDNGFQEVEGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSDPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRVYYSSDLTAPLSAWQKHNTDDSSLTTISGLTPDITYSLRVLGFTSVGDGPPSDILQVKTQQGVPAQPSSFEAEAELDSRIMLTWLWPVQDPITKYELQYWEAGSDNKLHVSFNPAGSYAVDGLKPDTLYRFSLAARSEMGLGVYTQPIEARTAQSTPSAPPQEVHLVSLSSTSLKVSWVAPPAASRHGAIVRYTVSYQAMAGEDTERHEVTGIGADATSFVLEGLEKWTEYQVWVRAHTDVGPGPESAPVRMRTKEDVPGAPPRKLEVEAINSTAIRVTWKPPLQGKQHGQIRGYQVIFSRLENGEPRGQPNIMDVALPEAQWNIEESTEHEAIIAGLHSETTYSVTVAAYTTKGDGARSKAKVITTTGAVPGKPTMMISTTMGNTALIQWQPPKEMVGELMGYQLQYKRTDEENYTSRELRKIDDHYTVTGLHKGATYVFRLSARNRAGIGEAYVKEISTPEDVPSGFPFNLQVTGLTQSSTELTWDPPLVAERNGKIVHYVVVYRDINSQQNSTNRTSETHMTIQGLNPDTTYDIRVQAFTSKGGGPLSPSIQSRTMSNDFPTLNFGVKAVMKTSVLLTWDLPPNYKSQVPFKILYNQQSVEVQGSLKRRLITQLQPDTDYSFVLTSRGNIAGGLQQQVSIRTAPDLINAKPSTHQPQGSKMTINLPKVQTTTRVRWYYIVVVPVSQSTQGWKNPDEMDLDELFKSSEGPVLRRRRHLDSSKPYIAAKLASLPTTFTLGDEKRYNGFYNKPLSSPQEYLCFVLAVLRSEGADSTTNYMTFSASPYSDPVHVRNSMAQGMEQPEMLWVMGPVLAVVLIIIIVIAILLFKNKQERKRASPLPKDEHSGGVKDSLLANSSDPVEMRRLNYQTPGMREHPPIPVVDLADHIERLKANDGLRFSQEYESIDPGQQFTWENSNMEVNKPKNRYANVIAYDHSRVVLTSVDAVPGSDYINANYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRSNTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVTMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRAQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPPPGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYLATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_019263543.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019263543.1

ORF Insert Sequence:

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTTGACATC 
AGCAGCAGCA ACGGACGCAT TAAACAGCTT CGTTCAGGTG GTACACCAAT TAGAGGAGCT 
CTGCAAATTG AGAACAGCGA GGAGTCCGAC CAAGGGAAAT ACGAGTGTGT TGCCATGAAC 
AGCGCTGGGA CCCGTTACTC CGCTCCTGCC AATCTCTACG TACGAGTGCG AAGAGTCCCA 
CCCAGATTCT CCATTCCGCC CACCAACCAA GAGGTGATGC CAGGCGGCAG CGTCAACCTG 
ACATGCGTGG CAGTGGGCTC GCCGATGCCC TACGTCAAGT GGACGATGGG CCTGGTGGAG 
CTGACCAAGG AGGAGGAGAT GCCGATGGGA CGCAACGTGC TGGAGGTCAA CAACATCCGC 
GAGTCGGCGA ACTACACCTG CGTGGCCATC TCCTCTCTGG GCATGATAGA AGCCACCGCT 
CAGGTCACCG TCAAAGCTTT GCCAAAGCCT CCCGCCTCCC TCATTGTGAC TGAGACCACA 
GCCACCAGTG TGACTCTCAC CTGGGACTCT GGAAACCCAG AACCTGTCTC CTACTACATG 
ATCCAATACA GAGCCAAGAC CTCGGACAAC GGCTTCCAGG AAGTGGAAGG TGTGGCAACT 
ACCCGTTACA GCATCGGTGG ACTTAGCCCC TACTCTGAGT ATGAGTTTCG TGTCATGGCT 
GTCAACAACA TCGGCCGGGG GCCACCGAGC GACCCCGTGG AGACACGCAC TGGTGAACAG 
GCCCCCTCCT CGCCACCTCT GCACGTCCAA GCGCGCATGC TGAGTGCCAG CACAATGCTG 
GTCCAGTGGG AACCGCCCGA GGAACCCAAC GGGCAAATCA GGGGCTACCG GGTCTACTAC 
AGCTCGGACT TGACGGCTCC GCTCAGCGCC TGGCAGAAGC ACAACACAGA CGACAGCAGC 
CTGACGACCA TCTCGGGCCT GACTCCTGAT ATCACCTACA GCCTCAGGGT GCTGGGCTTC 
ACCTCTGTAG GTGACGGGCC ACCTTCTGAC ATCCTGCAAG TCAAAACCCA ACAGGGAGTC 
CCTGCCCAGC CGTCCAGCTT TGAGGCCGAA GCTGAGCTGG ACAGTCGCAT CATGCTAACA 
TGGCTGTGGC CCGTCCAGGA CCCCATCACT AAGTATGAGC TGCAGTACTG GGAGGCCGGC 
TCAGACAACA AGTTACATGT GAGCTTCAAC CCAGCGGGCT CCTACGCTGT GGACGGTCTA 
AAGCCCGACA CCTTGTACCG TTTCTCTCTG GCAGCCCGTT CTGAGATGGG CTTAGGTGTC 
TACACCCAAC CCATCGAGGC TCGCACCGCC CAGTCCACCC CATCAGCTCC CCCCCAGGAG 
GTACACCTGG TCAGCCTGAG CTCCACCAGC CTCAAGGTGA GTTGGGTGGC ACCGCCCGCC 
GCTAGCCGCC ACGGCGCCAT TGTGCGCTAC ACAGTCAGCT ACCAGGCCAT GGCTGGAGAA 
GACACGGAGC GGCACGAGGT GACGGGCATC GGCGCAGACG CCACTAGCTT TGTGCTGGAG 
GGCCTGGAGA AGTGGACTGA GTATCAGGTG TGGGTGAGGG CACACACTGA CGTGGGCCCC 
GGCCCAGAGA GTGCCCCAGT GAGGATGAGA ACCAAAGAGG ATGTGCCGGG AGCACCCCCC 
AGGAAGCTGG AGGTTGAGGC CATAAACTCC ACAGCAATCC GGGTCACCTG GAAGCCGCCC 
CTGCAGGGGA AGCAGCACGG CCAAATCCGG GGCTATCAAG TCATCTTCTC TCGGCTTGAA 
AACGGCGAAC CACGAGGCCA GCCTAACATC ATGGACGTTG CTTTGCCAGA GGCACAGTGG 
AATATTGAGG AGTCCACCGA GCATGAGGCC ATCATTGCCG GACTGCACTC TGAAACCACC 
TACTCGGTCA CCGTGGCAGC ATACACCACC AAAGGAGACG GAGCGCGCAG CAAAGCCAAA 
GTCATCACCA CGACGGGTGC AGTGCCAGGA AAGCCCACCA TGATGATCAG CACCACTATG 
GGCAACACAG CGCTGATTCA GTGGCAGCCA CCTAAGGAAA TGGTTGGGGA GCTGATGGGC 
TACCAGCTGC AGTACAAGCG CACTGACGAG GAAAACTACA CCTCCCGCGA GCTGAGAAAG 
ATCGATGACC ACTACACCGT CACCGGGCTG CACAAAGGTG CCACCTATGT CTTCCGCCTC 
AGTGCCCGCA ACCGCGCGGG CATCGGCGAA GCATACGTGA AAGAGATCAG CACCCCTGAA 
GACGTGCCGT CGGGCTTCCC GTTCAACCTG CAGGTCACGG GACTGACTCA GTCCAGCACC 
GAGCTGACCT GGGACCCTCC GCTGGTGGCC GAAAGGAACG GCAAGATCGT GCACTATGTG 
GTCGTTTACA GGGACATCAA CAGTCAGCAG AACAGCACCA ACCGGACGAG CGAAACGCAC 
ATGACCATCC AGGGCCTTAA TCCCGACACC ACCTACGATA TCCGGGTGCA GGCGTTCACC 
AGTAAGGGCG GAGGACCCCT CAGCCCTAGC ATCCAGAGCA GGACCATGTC GAACGATTTT 
CCCACCCTCA ACTTTGGTGT CAAAGCCGTC ATGAAAACAT CTGTCCTCCT CACCTGGGAT 
TTGCCACCGA ACTACAAATC CCAAGTGCCT TTCAAGATCC TGTACAACCA GCAAAGCGTG 
GAAGTGCAGG GATCCCTGAA GAGGAGGCTG ATCACGCAGC TGCAGCCAGA CACGGACTAC 
TCTTTTGTGC TGACAAGCAG GGGCAACATT GCTGGTGGTC TACAGCAGCA GGTGTCCATC 
CGCACCGCTC CCGACCTGAT CAACGCCAAG CCCAGCACAC ATCAGCCTCA GGGTAGCAAG 
ATGACAATCA ACCTGCCCAA GGTCCAGACC ACCACGCGCG TCAGGTGGTA CTACATCGTG 
GTGGTGCCGG TGTCCCAGTC AACGCAGGGG TGGAAGAATC CAGACGAGAT GGACCTGGAC 
GAGCTGTTCA AGTCAAGTGA AGGCCCCGTC CTGAGGCGCA GACGTCACCT GGATTCCTCC 
AAACCTTACA TCGCTGCCAA GTTGGCCTCG TTGCCGACCA CCTTCACCCT GGGTGATGAG 
AAGAGATACA ACGGCTTCTA CAACAAGCCC CTGAGCAGTC CGCAGGAGTA TCTCTGCTTT 
GTCCTGGCAG TACTCCGATC CGAAGGGGCA GACAGCACTA CTAACTATAT GACATTTTCA 
GCCAGTCCCT ACTCAGACCC GGTCCACGTG CGAAACAGCA TGGCTCAGGG GATGGAGCAG 
CCTGAAATGT TGTGGGTTAT GGGCCCTGTT TTGGCCGTGG TCCTGATTAT CATCATCGTC 
ATCGCCATCC TGCTCTTCAA GAACAAACAG GAAAGGAAGC GAGCGTCGCC CCTGCCCAAA 
GATGAGCACT CGGGCGGGGT GAAGGACTCC CTGCTGGCCA ACTCCTCCGA CCCTGTGGAG 
ATGAGAAGAC TTAACTACCA GACCCCAGGA ATGAGGGAGC ACCCGCCCAT CCCTGTCGTC 
GACCTGGCTG ACCACATAGA GCGTCTGAAG GCCAACGACG GCCTACGCTT TTCCCAGGAG 
TACGAGTCGA TTGATCCAGG GCAGCAGTTT ACCTGGGAGA ACTCCAACAT GGAAGTCAAC 
AAGCCAAAGA ATCGCTATGC AAACGTCATT GCCTATGACC ACTCCAGAGT GGTTCTCACC 
TCTGTTGACG CCGTTCCAGG CAGTGACTAC ATCAACGCCA ACTACATCGA CGGCTACAGG 
AAGCAGAACG CCTACATCGC CACGCAGGGG CCACTTCCAG AGACACTGAG CGACTTCTGG 
AGGATGGTGT GGGAGCAAAG ATCCAACACC ATCGTCATGA TGACCAGACT GGAGGAGAAG 
TCACGGGTGA AGTGTGACCA GTACTGGCCG TCGCGAGGAA CGGAGACCTA TGGGATGATC 
CAGGTCACCA TGCTGGACAC CGTGGAGTTG GCCACTTACA GCGTCCGTAC CTTTGCCCTC 
TATAAGAATG GCTCCAGTGA GAAGAGAGAG GTGAGACAGT TCCAGTTCAT GGCCTGGCCA 
GACCACGGGG TGCCAGAGTA CCCCACCCCA ATCCTGGCCT TCCTACGGCG GGTAAAGGCC 
TGCAATCCTC CAGACGCAGG GCCTATGGTG GTCCACTGCA GCGCGGGTGT GGGCAGGACC 
GGATGCTTCA TCGTGATAGA TGCCATGCTG GAGCGCATGA AGCACGAGAA GTCCGTAGAC 
ATCTACGGCC ACGTGACGTG CATGCGCGCC CAGAGGAACT ACATGGTCCA GACCGAGGAC 
CAGTACATCT TCATTCACGA AGCCCTGCTG GAGGCCGCCA CGTGCGGCAA CACCGAGGTG 
CCGGCGCGCA ACCTGTACGC CCACATCCAG AAACTCACCC AGCCCCCGCC CGGCGAGACG 
GTTACTGCCA TGGAGCTGGA ATTCAAGAGA CTGGCCAACT CCAAAGCCCA CACATCCCGC 
TTCATCAGCG CCAACTTGCC CTGCAACAAG TTCAAGAACC GCCTGGTCAA CATCATGCCT 
TTCGAGTCCA CCCGTGTCTG CCTGCAGCCC ATCCGTGGCG TCGAGGGCTC CGACTACATC 
AATGCCAGCT GCATCGACGG CTACAGACAG CAGAAGGCCT ACCTGGCCAC GCAGGGCCCA 
CTGGCAGAGA CCACCGAGGA CTTCTGGAGG ATGCTGTGGG AGCACAACTC CACCATCGTC 
GTCATGCTCA CCAAGCTACG AGAGATGGGC CGGGAGAAGT GCCATCAGTA CTGGCCAGCA 
GAGCGTTCAG CCAGGTACCA GTACTTTGTG GTGGATCCAA TGGCTGAGTA CAACATGCCC 
CAGTACATCC TCAGAGAGTT CAAAGTCACA GATGCCAGGG ACGGCCAGTC CAGAACAATC 
CGCCAATTCC AGTTCACTGA CTGGCCCGAG CAAGGAGTGC CAAAAACCGG AGAGGGCTTC 
ATTGATTTTA TCGGACAAGT GCACAAAACC AAGGAACAGT TCGGACAGGA CGGACCAATC 
ACTGTACACT GCAGTGCTGG GGTGGGCAGG ACCGGAGTCT TCATCACCCT CAGCATCGTG 
CTGGAGAGGA TGAGGTATGA AGGAGTTGTC GACCTCTTCC AGACAGTGAA GACACTTCGC 
ACCCAGCGGC CTGCCATGGT GCAGACCGAG GACCAGTACC AGTTGTGCTA CAGGGCAGCA 
TTGGAGTACC TGGGGAGCTT TGACCACTAT GCAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104931439 ( XM_010746447.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010744749.2 Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X7, mRNA.

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CloneID	OLa84601
Clone ID Related Accession (Same CDS sequence)	XM_010746447.2
Accession Version	XM_010746447.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	receptor-type tyrosine-protein phosphatase F isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017607936.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 29, 2016 this sequence version replaced XM_010746447.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTTGACATC 
AGCAGCAGCA ACGGACGCAT TAAACAGCTT CGTTCAGGAG CTCTGCAAAT TGAGAACAGC 
GAGGAGTCCG ACCAAGGGAA ATACGAGTGT GTTGCCATGA ACAGCGCTGG GACCCGTTAC 
TCCGCTCCTG CCAATCTCTA CGTACGAGTG CGAAGAGTCC CACCCAGATT CTCCATTCCG 
CCCACCAACC AAGAGGTGAT GCCAGGCGGC AGCGTCAACC TGACATGCGT GGCAGTGGGC 
TCGCCGATGC CCTACGTCAA GTGGACGATG GGCCTGGTGG AGCTGACCAA GGAGGAGGAG 
ATGCCGATGG GACGCAACGT GCTGGAGGTC AACAACATCC GCGAGTCGGC GAACTACACC 
TGCGTGGCCA TCTCCTCTCT GGGCATGATA GAAGCCACCG CTCAGGTCAC CGTCAAAGCT 
TTGCCAAAGC CTCCCGCCTC CCTCATTGTG ACTGAGACCA CAGCCACCAG TGTGACTCTC 
ACCTGGGACT CTGGAAACCC AGAACCTGTC TCCTACTACA TGATCCAATA CAGAGCCAAG 
ACCTCGGACA ACGGCTTCCA GGAAGTGGAA GGTGTGGCAA CTACCCGTTA CAGCATCGGT 
GGACTTAGCC CCTACTCTGA GTATGAGTTT CGTGTCATGG CTGTCAACAA CATCGGCCGG 
GGGCCACCGA GCGACCCCGT GGAGACACGC ACTGGTGAAC AGGCCCCCTC CTCGCCACCT 
CTGCACGTCC AAGCGCGCAT GCTGAGTGCC AGCACAATGC TGGTCCAGTG GGAACCGCCC 
GAGGAACCCA ACGGGCAAAT CAGGGGCTAC CGGGTCTACT ACAGCTCGGA CTTGACGGCT 
CCGCTCAGCG CCTGGCAGAA GCACAACACA GACGACAGCA GCCTGACGAC CATCTCGGGC 
CTGACTCCTG ATATCACCTA CAGCCTCAGG GTGCTGGGCT TCACCTCTGT AGGTGACGGG 
CCACCTTCTG ACATCCTGCA AGTCAAAACC CAACAGGGAG TCCCTGCCCA GCCGTCCAGC 
TTTGAGGCCG AAGCTGAGCT GGACAGTCGC ATCATGCTAA CATGGCTGTG GCCCGTCCAG 
GACCCCATCA CTAAGTATGA GCTGCAGTAC TGGGAGGCCG GCTCAGACAA CAAGTTACAT 
GTGAGCTTCA ACCCAGCGGG CTCCTACGCT GTGGACGGTC TAAAGCCCGA CACCTTGTAC 
CGTTTCTCTC TGGCAGCCCG TTCTGAGATG GGCTTAGGTG TCTACACCCA ACCCATCGAG 
GCTCGCACCG CCCAGTCCAC CCCATCAGCT CCCCCCCAGG AGGTACACCT GGTCAGCCTG 
AGCTCCACCA GCCTCAAGGT GAGTTGGGTG GCACCGCCCG CCGCTAGCCG CCACGGCGCC 
ATTGTGCGCT ACACAGTCAG CTACCAGGCC ATGGCTGGAG AAGACACGGA GCGGCACGAG 
GTGACGGGCA TCGGCGCAGA CGCCACTAGC TTTGTGCTGG AGGGCCTGGA GAAGTGGACT 
GAGTATCAGG TGTGGGTGAG GGCACACACT GACGTGGGCC CCGGCCCAGA GAGTGCCCCA 
GTGAGGATGA GAACCAAAGA GGATGTGCCG GGAGCACCCC CCAGGAAGCT GGAGGTTGAG 
GCCATAAACT CCACAGCAAT CCGGGTCACC TGGAAGCCGC CCCTGCAGGG GAAGCAGCAC 
GGCCAAATCC GGGGCTATCA AGTCATCTTC TCTCGGCTTG AAAACGGCGA ACCACGAGGC 
CAGCCTAACA TCATGGACGT TGCTTTGCCA GAGGCACAGG AGGCCATCAT TGCCGGACTG 
CACTCTGAAA CCACCTACTC GGTCACCGTG GCAGCATACA CCACCAAAGG AGACGGAGCG 
CGCAGCAAAG CCAAAGTCAT CACCACGACG GGTGCAGTGC CAGGAAAGCC CACCATGATG 
ATCAGCACCA CTATGGGCAA CACAGCGCTG ATTCAGTGGC AGCCACCTAA GGAAATGGTT 
GGGGAGCTGA TGGGCTACCA GCTGCAGTAC AAGCGCACTG ACGAGGAAAA CTACACCTCC 
CGCGAGCTGA GAAAGATCGA TGACCACTAC ACCGTCACCG GGCTGCACAA AGGTGCCACC 
TATGTCTTCC GCCTCAGTGC CCGCAACCGC GCGGGCATCG GCGAAGCATA CGTGAAAGAG 
ATCAGCACCC CTGAAGACGT GCCGTCGGGC TTCCCGTTCA ACCTGCAGGT CACGGGACTG 
ACTCAGTCCA GCACCGAGCT GACCTGGGAC CCTCCGCTGG TGGCCGAAAG GAACGGCAAG 
ATCGTGCACT ATGTGGTCGT TTACAGGGAC ATCAACAGTC AGCAGAACAG CACCAACCGG 
ACGAGCGAAA CGCACATGAC CATCCAGGGC CTTAATCCCG ACACCACCTA CGATATCCGG 
GTGCAGGCGT TCACCAGTAA GGGCGGAGGA CCCCTCAGCC CTAGCATCCA GAGCAGGACC 
ATGTCGAACG ATTTTCCCAC CCTCAACTTT GGTGTCAAAG CCGTCATGAA AACATCTGTC 
CTCCTCACCT GGGATTTGCC ACCGAACTAC AAATCCCAAG TGCCTTTCAA GATCCTGTAC 
AACCAGCAAA GCGTGGAAGT GCAGGGATCC CTGAAGAGGA GGCTGATCAC GCAGCTGCAG 
CCAGACACGG ACTACTCTTT TGTGCTGACA AGCAGGGGCA ACATTGCTGG TGGTCTACAG 
CAGCAGGTGT CCATCCGCAC CGCTCCCGAC CTGATCAACG CCAAGCCCAG CACACATCAG 
CCTCAGGGTA GCAAGATGAC AATCAACCTG CCCAAGGTCC AGACCACCAC GCGCGTCAGG 
TGGTACTACA TCGTGGTGGT GCCGGTGTCC CAGTCAACGC AGGGGTGGAA GAATCCAGAC 
GAGATGGACC TGGACGAGCT GTTCAAGTCA AGTGAAGGCC CCGTCCTGAG GCGCAGACGT 
CACCTGGATT CCTCCAAACC TTACATCGCT GCCAAGTTGG CCTCGTTGCC GACCACCTTC 
ACCCTGGGTG ATGAGAAGAG ATACAACGGC TTCTACAACA AGCCCCTGAG CAGTCCGCAG 
GAGTATCTCT GCTTTGTCCT GGCAGTACTC CGATCCGAAG GGGCAGACAG CACTACTAAC 
TATATGACAT TTTCAGCCAG TCCCTACTCA GACCCGGTCC ACGTGCGAAA CAGCATGGCT 
CAGGGGATGG AGCAGCCTGA AATGTTGTGG GTTATGGGCC CTGTTTTGGC CGTGGTCCTG 
ATTATCATCA TCGTCATCGC CATCCTGCTC TTCAAGAACA AACAGGAAAG GAAGCGAGCG 
TCGCCCCTGC CCAAAGATGA GCACTCGGGC GGGGTGAAGG ACTCCCTGCT GGCCAACTCC 
TCCGACCCTG TGGAGATGAG AAGACTTAAC TACCAGACCC CAGGTCCCAG CTCTCATCGC 
TGCCCCAACA CTCCAAGAAT GAGGGAGCAC CCGCCCATCC CTGTCGTCGA CCTGGCTGAC 
CACATAGAGC GTCTGAAGGC CAACGACGGC CTACGCTTTT CCCAGGAGTA CGAGTCGATT 
GATCCAGGGC AGCAGTTTAC CTGGGAGAAC TCCAACATGG AAGTCAACAA GCCAAAGAAT 
CGCTATGCAA ACGTCATTGC CTATGACCAC TCCAGAGTGG TTCTCACCTC TGTTGACGCC 
GTTCCAGGCA GTGACTACAT CAACGCCAAC TACATCGACG GCTACAGGAA GCAGAACGCC 
TACATCGCCA CGCAGGGGCC ACTTCCAGAG ACACTGAGCG ACTTCTGGAG GATGGTGTGG 
GAGCAAAGAT CCAACACCAT CGTCATGATG ACCAGACTGG AGGAGAAGTC ACGGGTGAAG 
TGTGACCAGT ACTGGCCGTC GCGAGGAACG GAGACCTATG GGATGATCCA GGTCACCATG 
CTGGACACCG TGGAGTTGGC CACTTACAGC GTCCGTACCT TTGCCCTCTA TAAGAATGGC 
TCCAGTGAGA AGAGAGAGGT GAGACAGTTC CAGTTCATGG CCTGGCCAGA CCACGGGGTG 
CCAGAGTACC CCACCCCAAT CCTGGCCTTC CTACGGCGGG TAAAGGCCTG CAATCCTCCA 
GACGCAGGGC CTATGGTGGT CCACTGCAGC GCGGGTGTGG GCAGGACCGG ATGCTTCATC 
GTGATAGATG CCATGCTGGA GCGCATGAAG CACGAGAAGT CCGTAGACAT CTACGGCCAC 
GTGACGTGCA TGCGCGCCCA GAGGAACTAC ATGGTCCAGA CCGAGGACCA GTACATCTTC 
ATTCACGAAG CCCTGCTGGA GGCCGCCACG TGCGGCAACA CCGAGGTGCC GGCGCGCAAC 
CTGTACGCCC ACATCCAGAA ACTCACCCAG CCCCCGCCCG GCGAGACGGT TACTGCCATG 
GAGCTGGAAT TCAAGAGACT GGCCAACTCC AAAGCCCACA CATCCCGCTT CATCAGCGCC 
AACTTGCCCT GCAACAAGTT CAAGAACCGC CTGGTCAACA TCATGCCTTT CGAGTCCACC 
CGTGTCTGCC TGCAGCCCAT CCGTGGCGTC GAGGGCTCCG ACTACATCAA TGCCAGCTGC 
ATCGACGGCT ACAGACAGCA GAAGGCCTAC CTGGCCACGC AGGGCCCACT GGCAGAGACC 
ACCGAGGACT TCTGGAGGAT GCTGTGGGAG CACAACTCCA CCATCGTCGT CATGCTCACC 
AAGCTACGAG AGATGGGCCG GGAGAAGTGC CATCAGTACT GGCCAGCAGA GCGTTCAGCC 
AGGTACCAGT ACTTTGTGGT GGATCCAATG GCTGAGTACA ACATGCCCCA GTACATCCTC 
AGAGAGTTCA AAGTCACAGA TGCCAGGGAC GGCCAGTCCA GAACAATCCG CCAATTCCAG 
TTCACTGACT GGCCCGAGCA AGGAGTGCCA AAAACCGGAG AGGGCTTCAT TGATTTTATC 
GGACAAGTGC ACAAAACCAA GGAACAGTTC GGACAGGACG GACCAATCAC TGTACACTGC 
AGTGCTGGGG TGGGCAGGAC CGGAGTCTTC ATCACCCTCA GCATCGTGCT GGAGAGGATG 
AGGTATGAAG GAGTTGTCGA CCTCTTCCAG ACAGTGAAGA CACTTCGCAC CCAGCGGCCT 
GCCATGGTGC AGACCGAGGA CCAGTACCAG TTGTGCTACA GGGCAGCATT GGAGTACCTG 
GGGAGCTTTG ACCACTATGC AACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010744749.2
CDS	522..6248
Translation	MVPNIHLAVPFLPRGALLFLILLSCVVFSSLADSMPSFVKSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVEKTRTATMLCAASGNPDPEISWFKDMLPVDISSSNGRIKQLRSGALQIENSEESDQGKYECVAMNSAGTRYSAPANLYVRVRRVPPRFSIPPTNQEVMPGGSVNLTCVAVGSPMPYVKWTMGLVELTKEEEMPMGRNVLEVNNIRESANYTCVAISSLGMIEATAQVTVKALPKPPASLIVTETTATSVTLTWDSGNPEPVSYYMIQYRAKTSDNGFQEVEGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSDPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRVYYSSDLTAPLSAWQKHNTDDSSLTTISGLTPDITYSLRVLGFTSVGDGPPSDILQVKTQQGVPAQPSSFEAEAELDSRIMLTWLWPVQDPITKYELQYWEAGSDNKLHVSFNPAGSYAVDGLKPDTLYRFSLAARSEMGLGVYTQPIEARTAQSTPSAPPQEVHLVSLSSTSLKVSWVAPPAASRHGAIVRYTVSYQAMAGEDTERHEVTGIGADATSFVLEGLEKWTEYQVWVRAHTDVGPGPESAPVRMRTKEDVPGAPPRKLEVEAINSTAIRVTWKPPLQGKQHGQIRGYQVIFSRLENGEPRGQPNIMDVALPEAQEAIIAGLHSETTYSVTVAAYTTKGDGARSKAKVITTTGAVPGKPTMMISTTMGNTALIQWQPPKEMVGELMGYQLQYKRTDEENYTSRELRKIDDHYTVTGLHKGATYVFRLSARNRAGIGEAYVKEISTPEDVPSGFPFNLQVTGLTQSSTELTWDPPLVAERNGKIVHYVVVYRDINSQQNSTNRTSETHMTIQGLNPDTTYDIRVQAFTSKGGGPLSPSIQSRTMSNDFPTLNFGVKAVMKTSVLLTWDLPPNYKSQVPFKILYNQQSVEVQGSLKRRLITQLQPDTDYSFVLTSRGNIAGGLQQQVSIRTAPDLINAKPSTHQPQGSKMTINLPKVQTTTRVRWYYIVVVPVSQSTQGWKNPDEMDLDELFKSSEGPVLRRRRHLDSSKPYIAAKLASLPTTFTLGDEKRYNGFYNKPLSSPQEYLCFVLAVLRSEGADSTTNYMTFSASPYSDPVHVRNSMAQGMEQPEMLWVMGPVLAVVLIIIIVIAILLFKNKQERKRASPLPKDEHSGGVKDSLLANSSDPVEMRRLNYQTPGPSSHRCPNTPRMREHPPIPVVDLADHIERLKANDGLRFSQEYESIDPGQQFTWENSNMEVNKPKNRYANVIAYDHSRVVLTSVDAVPGSDYINANYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRSNTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVTMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRAQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPPPGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYLATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_010746447.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010746447.2

ORF Insert Sequence:

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTTGACATC 
AGCAGCAGCA ACGGACGCAT TAAACAGCTT CGTTCAGGAG CTCTGCAAAT TGAGAACAGC 
GAGGAGTCCG ACCAAGGGAA ATACGAGTGT GTTGCCATGA ACAGCGCTGG GACCCGTTAC 
TCCGCTCCTG CCAATCTCTA CGTACGAGTG CGAAGAGTCC CACCCAGATT CTCCATTCCG 
CCCACCAACC AAGAGGTGAT GCCAGGCGGC AGCGTCAACC TGACATGCGT GGCAGTGGGC 
TCGCCGATGC CCTACGTCAA GTGGACGATG GGCCTGGTGG AGCTGACCAA GGAGGAGGAG 
ATGCCGATGG GACGCAACGT GCTGGAGGTC AACAACATCC GCGAGTCGGC GAACTACACC 
TGCGTGGCCA TCTCCTCTCT GGGCATGATA GAAGCCACCG CTCAGGTCAC CGTCAAAGCT 
TTGCCAAAGC CTCCCGCCTC CCTCATTGTG ACTGAGACCA CAGCCACCAG TGTGACTCTC 
ACCTGGGACT CTGGAAACCC AGAACCTGTC TCCTACTACA TGATCCAATA CAGAGCCAAG 
ACCTCGGACA ACGGCTTCCA GGAAGTGGAA GGTGTGGCAA CTACCCGTTA CAGCATCGGT 
GGACTTAGCC CCTACTCTGA GTATGAGTTT CGTGTCATGG CTGTCAACAA CATCGGCCGG 
GGGCCACCGA GCGACCCCGT GGAGACACGC ACTGGTGAAC AGGCCCCCTC CTCGCCACCT 
CTGCACGTCC AAGCGCGCAT GCTGAGTGCC AGCACAATGC TGGTCCAGTG GGAACCGCCC 
GAGGAACCCA ACGGGCAAAT CAGGGGCTAC CGGGTCTACT ACAGCTCGGA CTTGACGGCT 
CCGCTCAGCG CCTGGCAGAA GCACAACACA GACGACAGCA GCCTGACGAC CATCTCGGGC 
CTGACTCCTG ATATCACCTA CAGCCTCAGG GTGCTGGGCT TCACCTCTGT AGGTGACGGG 
CCACCTTCTG ACATCCTGCA AGTCAAAACC CAACAGGGAG TCCCTGCCCA GCCGTCCAGC 
TTTGAGGCCG AAGCTGAGCT GGACAGTCGC ATCATGCTAA CATGGCTGTG GCCCGTCCAG 
GACCCCATCA CTAAGTATGA GCTGCAGTAC TGGGAGGCCG GCTCAGACAA CAAGTTACAT 
GTGAGCTTCA ACCCAGCGGG CTCCTACGCT GTGGACGGTC TAAAGCCCGA CACCTTGTAC 
CGTTTCTCTC TGGCAGCCCG TTCTGAGATG GGCTTAGGTG TCTACACCCA ACCCATCGAG 
GCTCGCACCG CCCAGTCCAC CCCATCAGCT CCCCCCCAGG AGGTACACCT GGTCAGCCTG 
AGCTCCACCA GCCTCAAGGT GAGTTGGGTG GCACCGCCCG CCGCTAGCCG CCACGGCGCC 
ATTGTGCGCT ACACAGTCAG CTACCAGGCC ATGGCTGGAG AAGACACGGA GCGGCACGAG 
GTGACGGGCA TCGGCGCAGA CGCCACTAGC TTTGTGCTGG AGGGCCTGGA GAAGTGGACT 
GAGTATCAGG TGTGGGTGAG GGCACACACT GACGTGGGCC CCGGCCCAGA GAGTGCCCCA 
GTGAGGATGA GAACCAAAGA GGATGTGCCG GGAGCACCCC CCAGGAAGCT GGAGGTTGAG 
GCCATAAACT CCACAGCAAT CCGGGTCACC TGGAAGCCGC CCCTGCAGGG GAAGCAGCAC 
GGCCAAATCC GGGGCTATCA AGTCATCTTC TCTCGGCTTG AAAACGGCGA ACCACGAGGC 
CAGCCTAACA TCATGGACGT TGCTTTGCCA GAGGCACAGG AGGCCATCAT TGCCGGACTG 
CACTCTGAAA CCACCTACTC GGTCACCGTG GCAGCATACA CCACCAAAGG AGACGGAGCG 
CGCAGCAAAG CCAAAGTCAT CACCACGACG GGTGCAGTGC CAGGAAAGCC CACCATGATG 
ATCAGCACCA CTATGGGCAA CACAGCGCTG ATTCAGTGGC AGCCACCTAA GGAAATGGTT 
GGGGAGCTGA TGGGCTACCA GCTGCAGTAC AAGCGCACTG ACGAGGAAAA CTACACCTCC 
CGCGAGCTGA GAAAGATCGA TGACCACTAC ACCGTCACCG GGCTGCACAA AGGTGCCACC 
TATGTCTTCC GCCTCAGTGC CCGCAACCGC GCGGGCATCG GCGAAGCATA CGTGAAAGAG 
ATCAGCACCC CTGAAGACGT GCCGTCGGGC TTCCCGTTCA ACCTGCAGGT CACGGGACTG 
ACTCAGTCCA GCACCGAGCT GACCTGGGAC CCTCCGCTGG TGGCCGAAAG GAACGGCAAG 
ATCGTGCACT ATGTGGTCGT TTACAGGGAC ATCAACAGTC AGCAGAACAG CACCAACCGG 
ACGAGCGAAA CGCACATGAC CATCCAGGGC CTTAATCCCG ACACCACCTA CGATATCCGG 
GTGCAGGCGT TCACCAGTAA GGGCGGAGGA CCCCTCAGCC CTAGCATCCA GAGCAGGACC 
ATGTCGAACG ATTTTCCCAC CCTCAACTTT GGTGTCAAAG CCGTCATGAA AACATCTGTC 
CTCCTCACCT GGGATTTGCC ACCGAACTAC AAATCCCAAG TGCCTTTCAA GATCCTGTAC 
AACCAGCAAA GCGTGGAAGT GCAGGGATCC CTGAAGAGGA GGCTGATCAC GCAGCTGCAG 
CCAGACACGG ACTACTCTTT TGTGCTGACA AGCAGGGGCA ACATTGCTGG TGGTCTACAG 
CAGCAGGTGT CCATCCGCAC CGCTCCCGAC CTGATCAACG CCAAGCCCAG CACACATCAG 
CCTCAGGGTA GCAAGATGAC AATCAACCTG CCCAAGGTCC AGACCACCAC GCGCGTCAGG 
TGGTACTACA TCGTGGTGGT GCCGGTGTCC CAGTCAACGC AGGGGTGGAA GAATCCAGAC 
GAGATGGACC TGGACGAGCT GTTCAAGTCA AGTGAAGGCC CCGTCCTGAG GCGCAGACGT 
CACCTGGATT CCTCCAAACC TTACATCGCT GCCAAGTTGG CCTCGTTGCC GACCACCTTC 
ACCCTGGGTG ATGAGAAGAG ATACAACGGC TTCTACAACA AGCCCCTGAG CAGTCCGCAG 
GAGTATCTCT GCTTTGTCCT GGCAGTACTC CGATCCGAAG GGGCAGACAG CACTACTAAC 
TATATGACAT TTTCAGCCAG TCCCTACTCA GACCCGGTCC ACGTGCGAAA CAGCATGGCT 
CAGGGGATGG AGCAGCCTGA AATGTTGTGG GTTATGGGCC CTGTTTTGGC CGTGGTCCTG 
ATTATCATCA TCGTCATCGC CATCCTGCTC TTCAAGAACA AACAGGAAAG GAAGCGAGCG 
TCGCCCCTGC CCAAAGATGA GCACTCGGGC GGGGTGAAGG ACTCCCTGCT GGCCAACTCC 
TCCGACCCTG TGGAGATGAG AAGACTTAAC TACCAGACCC CAGGTCCCAG CTCTCATCGC 
TGCCCCAACA CTCCAAGAAT GAGGGAGCAC CCGCCCATCC CTGTCGTCGA CCTGGCTGAC 
CACATAGAGC GTCTGAAGGC CAACGACGGC CTACGCTTTT CCCAGGAGTA CGAGTCGATT 
GATCCAGGGC AGCAGTTTAC CTGGGAGAAC TCCAACATGG AAGTCAACAA GCCAAAGAAT 
CGCTATGCAA ACGTCATTGC CTATGACCAC TCCAGAGTGG TTCTCACCTC TGTTGACGCC 
GTTCCAGGCA GTGACTACAT CAACGCCAAC TACATCGACG GCTACAGGAA GCAGAACGCC 
TACATCGCCA CGCAGGGGCC ACTTCCAGAG ACACTGAGCG ACTTCTGGAG GATGGTGTGG 
GAGCAAAGAT CCAACACCAT CGTCATGATG ACCAGACTGG AGGAGAAGTC ACGGGTGAAG 
TGTGACCAGT ACTGGCCGTC GCGAGGAACG GAGACCTATG GGATGATCCA GGTCACCATG 
CTGGACACCG TGGAGTTGGC CACTTACAGC GTCCGTACCT TTGCCCTCTA TAAGAATGGC 
TCCAGTGAGA AGAGAGAGGT GAGACAGTTC CAGTTCATGG CCTGGCCAGA CCACGGGGTG 
CCAGAGTACC CCACCCCAAT CCTGGCCTTC CTACGGCGGG TAAAGGCCTG CAATCCTCCA 
GACGCAGGGC CTATGGTGGT CCACTGCAGC GCGGGTGTGG GCAGGACCGG ATGCTTCATC 
GTGATAGATG CCATGCTGGA GCGCATGAAG CACGAGAAGT CCGTAGACAT CTACGGCCAC 
GTGACGTGCA TGCGCGCCCA GAGGAACTAC ATGGTCCAGA CCGAGGACCA GTACATCTTC 
ATTCACGAAG CCCTGCTGGA GGCCGCCACG TGCGGCAACA CCGAGGTGCC GGCGCGCAAC 
CTGTACGCCC ACATCCAGAA ACTCACCCAG CCCCCGCCCG GCGAGACGGT TACTGCCATG 
GAGCTGGAAT TCAAGAGACT GGCCAACTCC AAAGCCCACA CATCCCGCTT CATCAGCGCC 
AACTTGCCCT GCAACAAGTT CAAGAACCGC CTGGTCAACA TCATGCCTTT CGAGTCCACC 
CGTGTCTGCC TGCAGCCCAT CCGTGGCGTC GAGGGCTCCG ACTACATCAA TGCCAGCTGC 
ATCGACGGCT ACAGACAGCA GAAGGCCTAC CTGGCCACGC AGGGCCCACT GGCAGAGACC 
ACCGAGGACT TCTGGAGGAT GCTGTGGGAG CACAACTCCA CCATCGTCGT CATGCTCACC 
AAGCTACGAG AGATGGGCCG GGAGAAGTGC CATCAGTACT GGCCAGCAGA GCGTTCAGCC 
AGGTACCAGT ACTTTGTGGT GGATCCAATG GCTGAGTACA ACATGCCCCA GTACATCCTC 
AGAGAGTTCA AAGTCACAGA TGCCAGGGAC GGCCAGTCCA GAACAATCCG CCAATTCCAG 
TTCACTGACT GGCCCGAGCA AGGAGTGCCA AAAACCGGAG AGGGCTTCAT TGATTTTATC 
GGACAAGTGC ACAAAACCAA GGAACAGTTC GGACAGGACG GACCAATCAC TGTACACTGC 
AGTGCTGGGG TGGGCAGGAC CGGAGTCTTC ATCACCCTCA GCATCGTGCT GGAGAGGATG 
AGGTATGAAG GAGTTGTCGA CCTCTTCCAG ACAGTGAAGA CACTTCGCAC CCAGCGGCCT 
GCCATGGTGC AGACCGAGGA CCAGTACCAG TTGTGCTACA GGGCAGCATT GGAGTACCTG 
GGGAGCTTTG ACCACTATGC AACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104931439 ( XM_019263557.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019119102.1 Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X8, mRNA.

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CloneID	OLa84602
Clone ID Related Accession (Same CDS sequence)	XM_019263557.1
Accession Version	XM_019263557.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	receptor-type tyrosine-protein phosphatase F isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017607936.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTTGACATC 
AGCAGCAGCA ACGGACGCAT TAAACAGCTT CGTTCAGGTG GTACACCAAT TAGAGGAGCT 
CTGCAAATTG AGAACAGCGA GGAGTCCGAC CAAGGGAAAT ACGAGTGTGT TGCCATGAAC 
AGCGCTGGGA CCCGTTACTC CGCTCCTGCC AATCTCTACG TACGAGTGCG AAGAGTCCCA 
CCCAGATTCT CCATTCCGCC CACCAACCAA GAGGTGATGC CAGGCGGCAG CGTCAACCTG 
ACATGCGTGG CAGTGGGCTC GCCGATGCCC TACGTCAAGT GGACGATGGG CCTGGTGGAG 
CTGACCAAGG AGGAGGAGAT GCCGATGGGA CGCAACGTGC TGGAGGTCAA CAACATCCGC 
GAGTCGGCGA ACTACACCTG CGTGGCCATC TCCTCTCTGG GCATGATAGA AGCCACCGCT 
CAGGTCACCG TCAAAGCTTT GCCAAAGCCT CCCGCCTCCC TCATTGTGAC TGAGACCACA 
GCCACCAGTG TGACTCTCAC CTGGGACTCT GGAAACCCAG AACCTGTCTC CTACTACATG 
ATCCAATACA GAGCCAAGAC CTCGGACAAC GGCTTCCAGG AAGTGGAAGG TGTGGCAACT 
ACCCGTTACA GCATCGGTGG ACTTAGCCCC TACTCTGAGT ATGAGTTTCG TGTCATGGCT 
GTCAACAACA TCGGCCGGGG GCCACCGAGC GACCCCGTGG AGACACGCAC TGGTGAACAG 
GCCCCCTCCT CGCCACCTCT GCACGTCCAA GCGCGCATGC TGAGTGCCAG CACAATGCTG 
GTCCAGTGGG AACCGCCCGA GGAACCCAAC GGGCAAATCA GGGGCTACCG GGTCTACTAC 
AGCTCGGACT TGACGGCTCC GCTCAGCGCC TGGCAGAAGC ACAACACAGA CGACAGCAGC 
CTGACGACCA TCTCGGGCCT GACTCCTGAT ATCACCTACA GCCTCAGGGT GCTGGGCTTC 
ACCTCTGTAG GTGACGGGCC ACCTTCTGAC ATCCTGCAAG TCAAAACCCA ACAGGGAGTC 
CCTGCCCAGC CGTCCAGCTT TGAGGCCGAA GCTGAGCTGG ACAGTCGCAT CATGCTAACA 
TGGCTGTGGC CCGTCCAGGA CCCCATCACT AAGTATGAGC TGCAGTACTG GGAGGCCGGC 
TCAGACAACA AGTTACATGT GAGCTTCAAC CCAGCGGGCT CCTACGCTGT GGACGGTCTA 
AAGCCCGACA CCTTGTACCG TTTCTCTCTG GCAGCCCGTT CTGAGATGGG CTTAGGTGTC 
TACACCCAAC CCATCGAGGC TCGCACCGCC CAGTCCACCC CATCAGCTCC CCCCCAGGAG 
GTACACCTGG TCAGCCTGAG CTCCACCAGC CTCAAGGTGA GTTGGGTGGC ACCGCCCGCC 
GCTAGCCGCC ACGGCGCCAT TGTGCGCTAC ACAGTCAGCT ACCAGGCCAT GGCTGGAGAA 
GACACGGAGC GGCACGAGGT GACGGGCATC GGCGCAGACG CCACTAGCTT TGTGCTGGAG 
GGCCTGGAGA AGTGGACTGA GTATCAGGTG TGGGTGAGGG CACACACTGA CGTGGGCCCC 
GGCCCAGAGA GTGCCCCAGT GAGGATGAGA ACCAAAGAGG ATGTGCCGGG AGCACCCCCC 
AGGAAGCTGG AGGTTGAGGC CATAAACTCC ACAGCAATCC GGGTCACCTG GAAGCCGCCC 
CTGCAGGGGA AGCAGCACGG CCAAATCCGG GGCTATCAAG TCATCTTCTC TCGGCTTGAA 
AACGGCGAAC CACGAGGCCA GCCTAACATC ATGGACGTTG CTTTGCCAGA GGCACAGTGG 
AATATTGAGG AGTCCACCGA GCATGAGGCC ATCATTGCCG GACTGCACTC TGAAACCACC 
TACTCGGTCA CCGTGGCAGC ATACACCACC AAAGGAGACG GAGCGCGCAG CAAAGCCAAA 
GTCATCACCA CGACGGGTGC AGTGCCAGGA AAGCCCACCA TGATGATCAG CACCACTATG 
GGCAACACAG CGCTGATTCA GTGGCAGCCA CCTAAGGAAA TGGTTGGGGA GCTGATGGGC 
TACCAGCTGC AGTACAAGCG CACTGACGAG GAAAACTACA CCTCCCGCGA GCTGAGAAAG 
ATCGATGACC ACTACACCGT CACCGGGCTG CACAAAGGTG CCACCTATGT CTTCCGCCTC 
AGTGCCCGCA ACCGCGCGGG CATCGGCGAA GCATACGTGA AAGAGATCAG CACCCCTGAA 
GACGTGCCGT CGGGCTTCCC GTTCAACCTG CAGGTCACGG GACTGACTCA GTCCAGCACC 
GAGCTGACCT GGGACCCTCC GCTGGTGGCC GAAAGGAACG GCAAGATCGT GCACTATGTG 
GTCGTTTACA GGGACATCAA CAGTCAGCAG AACAGCACCA ACCGGACGAG CGAAACGCAC 
ATGACCATCC AGGGCCTTAA TCCCGACACC ACCTACGATA TCCGGGTGCA GGCGTTCACC 
AGTAAGGGCG GAGGACCCCT CAGCCCTAGC ATCCAGAGCA GGACCATGTC GAACGATTTT 
CCCACCCTCA ACTTTGGTGT CAAAGCCGTC ATGAAAACAT CTGTCCTCCT CACCTGGGAT 
TTGCCACCGA ACTACAAATC CCAAGTGCCT TTCAAGATCC TGTACAACCA GCAAAGCGTG 
GAAGTGCAGG GATCCCTGAA GAGGAGGCTG ATCACGCAGC TGCAGCCAGA CACGGACTAC 
TCTTTTGTGC TGACAAGCAG GGGCAACATT GCTGGTGGTC TACAGCAGCA GGTGTCCATC 
CGCACCGCTC CCGACCTGAT CAACGCCAAG CCCAGCACAC ATCAGCCTCA GGGTAGCAAG 
ATGACAATCA ACCTGCCCAA GGTCCAGACC ACCACGCGCG TCAGGTGGTA CTACATCGTG 
GTGGTGCCGG TGTCCCAGTC AACGCAGGGG TGGAAGAATC CAGACGAGAT GGACCTGGAC 
GAGCTGTTCA AGTCAAGTGA AGGCCCCGTC CTGAGGCGCA GACGTCACCT GGATTCCTCC 
AAACCTTACA TCGCTGCCAA GTTGGCCTCG TTGCCGACCA CCTTCACCCT GGGTGATGAG 
AAGAGATACA ACGGCTTCTA CAACAAGCCC CTGAGCAGTC CGCAGGAGTA TCTCTGCTTT 
GTCCTGGCAG TACTCCGATC CGAAGGGGCA GACAGCACTA CTAACTATAT GACATTTTCA 
GCCAGTCCCT ACTCAGACCC GGTCCACGTG CGAAACAGCA TGGCTCAGGG GATGGAGCAG 
CCTGAAATGT TGTGGGTTAT GGGCCCTGTT TTGGCCGTGG TCCTGATTAT CATCATCGTC 
ATCGCCATCC TGCTCTTCAA GAAGAAGCGA GCGTCGCCCC TGCCCAAAGA TGAGCACTCG 
GGCGGGGTGA AGGACTCCCT GCTGGCCAAC TCCTCCGACC CTGTGGAGAT GAGAAGACTT 
AACTACCAGA CCCCAGGAAT GAGGGAGCAC CCGCCCATCC CTGTCGTCGA CCTGGCTGAC 
CACATAGAGC GTCTGAAGGC CAACGACGGC CTACGCTTTT CCCAGGAGTA CGAGTCGATT 
GATCCAGGGC AGCAGTTTAC CTGGGAGAAC TCCAACATGG AAGTCAACAA GCCAAAGAAT 
CGCTATGCAA ACGTCATTGC CTATGACCAC TCCAGAGTGG TTCTCACCTC TGTTGACGCC 
GTTCCAGGCA GTGACTACAT CAACGCCAAC TACATCGACG GCTACAGGAA GCAGAACGCC 
TACATCGCCA CGCAGGGGCC ACTTCCAGAG ACACTGAGCG ACTTCTGGAG GATGGTGTGG 
GAGCAAAGAT CCAACACCAT CGTCATGATG ACCAGACTGG AGGAGAAGTC ACGGGTGAAG 
TGTGACCAGT ACTGGCCGTC GCGAGGAACG GAGACCTATG GGATGATCCA GGTCACCATG 
CTGGACACCG TGGAGTTGGC CACTTACAGC GTCCGTACCT TTGCCCTCTA TAAGAATGGC 
TCCAGTGAGA AGAGAGAGGT GAGACAGTTC CAGTTCATGG CCTGGCCAGA CCACGGGGTG 
CCAGAGTACC CCACCCCAAT CCTGGCCTTC CTACGGCGGG TAAAGGCCTG CAATCCTCCA 
GACGCAGGGC CTATGGTGGT CCACTGCAGC GCGGGTGTGG GCAGGACCGG ATGCTTCATC 
GTGATAGATG CCATGCTGGA GCGCATGAAG CACGAGAAGT CCGTAGACAT CTACGGCCAC 
GTGACGTGCA TGCGCGCCCA GAGGAACTAC ATGGTCCAGA CCGAGGACCA GTACATCTTC 
ATTCACGAAG CCCTGCTGGA GGCCGCCACG TGCGGCAACA CCGAGGTGCC GGCGCGCAAC 
CTGTACGCCC ACATCCAGAA ACTCACCCAG CCCCCGCCCG GCGAGACGGT TACTGCCATG 
GAGCTGGAAT TCAAGAGACT GGCCAACTCC AAAGCCCACA CATCCCGCTT CATCAGCGCC 
AACTTGCCCT GCAACAAGTT CAAGAACCGC CTGGTCAACA TCATGCCTTT CGAGTCCACC 
CGTGTCTGCC TGCAGCCCAT CCGTGGCGTC GAGGGCTCCG ACTACATCAA TGCCAGCTGC 
ATCGACGGCT ACAGACAGCA GAAGGCCTAC CTGGCCACGC AGGGCCCACT GGCAGAGACC 
ACCGAGGACT TCTGGAGGAT GCTGTGGGAG CACAACTCCA CCATCGTCGT CATGCTCACC 
AAGCTACGAG AGATGGGCCG GGAGAAGTGC CATCAGTACT GGCCAGCAGA GCGTTCAGCC 
AGGTACCAGT ACTTTGTGGT GGATCCAATG GCTGAGTACA ACATGCCCCA GTACATCCTC 
AGAGAGTTCA AAGTCACAGA TGCCAGGGAC GGCCAGTCCA GAACAATCCG CCAATTCCAG 
TTCACTGACT GGCCCGAGCA AGGAGTGCCA AAAACCGGAG AGGGCTTCAT TGATTTTATC 
GGACAAGTGC ACAAAACCAA GGAACAGTTC GGACAGGACG GACCAATCAC TGTACACTGC 
AGTGCTGGGG TGGGCAGGAC CGGAGTCTTC ATCACCCTCA GCATCGTGCT GGAGAGGATG 
AGGTATGAAG GAGTTGTCGA CCTCTTCCAG ACAGTGAAGA CACTTCGCAC CCAGCGGCCT 
GCCATGGTGC AGACCGAGGA CCAGTACCAG TTGTGCTACA GGGCAGCATT GGAGTACCTG 
GGGAGCTTTG ACCACTATGC AACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019119102.1
CDS	522..6248
Translation	MVPNIHLAVPFLPRGALLFLILLSCVVFSSLADSMPSFVKSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVEKTRTATMLCAASGNPDPEISWFKDMLPVDISSSNGRIKQLRSGGTPIRGALQIENSEESDQGKYECVAMNSAGTRYSAPANLYVRVRRVPPRFSIPPTNQEVMPGGSVNLTCVAVGSPMPYVKWTMGLVELTKEEEMPMGRNVLEVNNIRESANYTCVAISSLGMIEATAQVTVKALPKPPASLIVTETTATSVTLTWDSGNPEPVSYYMIQYRAKTSDNGFQEVEGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSDPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRVYYSSDLTAPLSAWQKHNTDDSSLTTISGLTPDITYSLRVLGFTSVGDGPPSDILQVKTQQGVPAQPSSFEAEAELDSRIMLTWLWPVQDPITKYELQYWEAGSDNKLHVSFNPAGSYAVDGLKPDTLYRFSLAARSEMGLGVYTQPIEARTAQSTPSAPPQEVHLVSLSSTSLKVSWVAPPAASRHGAIVRYTVSYQAMAGEDTERHEVTGIGADATSFVLEGLEKWTEYQVWVRAHTDVGPGPESAPVRMRTKEDVPGAPPRKLEVEAINSTAIRVTWKPPLQGKQHGQIRGYQVIFSRLENGEPRGQPNIMDVALPEAQWNIEESTEHEAIIAGLHSETTYSVTVAAYTTKGDGARSKAKVITTTGAVPGKPTMMISTTMGNTALIQWQPPKEMVGELMGYQLQYKRTDEENYTSRELRKIDDHYTVTGLHKGATYVFRLSARNRAGIGEAYVKEISTPEDVPSGFPFNLQVTGLTQSSTELTWDPPLVAERNGKIVHYVVVYRDINSQQNSTNRTSETHMTIQGLNPDTTYDIRVQAFTSKGGGPLSPSIQSRTMSNDFPTLNFGVKAVMKTSVLLTWDLPPNYKSQVPFKILYNQQSVEVQGSLKRRLITQLQPDTDYSFVLTSRGNIAGGLQQQVSIRTAPDLINAKPSTHQPQGSKMTINLPKVQTTTRVRWYYIVVVPVSQSTQGWKNPDEMDLDELFKSSEGPVLRRRRHLDSSKPYIAAKLASLPTTFTLGDEKRYNGFYNKPLSSPQEYLCFVLAVLRSEGADSTTNYMTFSASPYSDPVHVRNSMAQGMEQPEMLWVMGPVLAVVLIIIIVIAILLFKKKRASPLPKDEHSGGVKDSLLANSSDPVEMRRLNYQTPGMREHPPIPVVDLADHIERLKANDGLRFSQEYESIDPGQQFTWENSNMEVNKPKNRYANVIAYDHSRVVLTSVDAVPGSDYINANYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRSNTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVTMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRAQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPPPGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYLATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_019263557.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019263557.1

ORF Insert Sequence:

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTTGACATC 
AGCAGCAGCA ACGGACGCAT TAAACAGCTT CGTTCAGGTG GTACACCAAT TAGAGGAGCT 
CTGCAAATTG AGAACAGCGA GGAGTCCGAC CAAGGGAAAT ACGAGTGTGT TGCCATGAAC 
AGCGCTGGGA CCCGTTACTC CGCTCCTGCC AATCTCTACG TACGAGTGCG AAGAGTCCCA 
CCCAGATTCT CCATTCCGCC CACCAACCAA GAGGTGATGC CAGGCGGCAG CGTCAACCTG 
ACATGCGTGG CAGTGGGCTC GCCGATGCCC TACGTCAAGT GGACGATGGG CCTGGTGGAG 
CTGACCAAGG AGGAGGAGAT GCCGATGGGA CGCAACGTGC TGGAGGTCAA CAACATCCGC 
GAGTCGGCGA ACTACACCTG CGTGGCCATC TCCTCTCTGG GCATGATAGA AGCCACCGCT 
CAGGTCACCG TCAAAGCTTT GCCAAAGCCT CCCGCCTCCC TCATTGTGAC TGAGACCACA 
GCCACCAGTG TGACTCTCAC CTGGGACTCT GGAAACCCAG AACCTGTCTC CTACTACATG 
ATCCAATACA GAGCCAAGAC CTCGGACAAC GGCTTCCAGG AAGTGGAAGG TGTGGCAACT 
ACCCGTTACA GCATCGGTGG ACTTAGCCCC TACTCTGAGT ATGAGTTTCG TGTCATGGCT 
GTCAACAACA TCGGCCGGGG GCCACCGAGC GACCCCGTGG AGACACGCAC TGGTGAACAG 
GCCCCCTCCT CGCCACCTCT GCACGTCCAA GCGCGCATGC TGAGTGCCAG CACAATGCTG 
GTCCAGTGGG AACCGCCCGA GGAACCCAAC GGGCAAATCA GGGGCTACCG GGTCTACTAC 
AGCTCGGACT TGACGGCTCC GCTCAGCGCC TGGCAGAAGC ACAACACAGA CGACAGCAGC 
CTGACGACCA TCTCGGGCCT GACTCCTGAT ATCACCTACA GCCTCAGGGT GCTGGGCTTC 
ACCTCTGTAG GTGACGGGCC ACCTTCTGAC ATCCTGCAAG TCAAAACCCA ACAGGGAGTC 
CCTGCCCAGC CGTCCAGCTT TGAGGCCGAA GCTGAGCTGG ACAGTCGCAT CATGCTAACA 
TGGCTGTGGC CCGTCCAGGA CCCCATCACT AAGTATGAGC TGCAGTACTG GGAGGCCGGC 
TCAGACAACA AGTTACATGT GAGCTTCAAC CCAGCGGGCT CCTACGCTGT GGACGGTCTA 
AAGCCCGACA CCTTGTACCG TTTCTCTCTG GCAGCCCGTT CTGAGATGGG CTTAGGTGTC 
TACACCCAAC CCATCGAGGC TCGCACCGCC CAGTCCACCC CATCAGCTCC CCCCCAGGAG 
GTACACCTGG TCAGCCTGAG CTCCACCAGC CTCAAGGTGA GTTGGGTGGC ACCGCCCGCC 
GCTAGCCGCC ACGGCGCCAT TGTGCGCTAC ACAGTCAGCT ACCAGGCCAT GGCTGGAGAA 
GACACGGAGC GGCACGAGGT GACGGGCATC GGCGCAGACG CCACTAGCTT TGTGCTGGAG 
GGCCTGGAGA AGTGGACTGA GTATCAGGTG TGGGTGAGGG CACACACTGA CGTGGGCCCC 
GGCCCAGAGA GTGCCCCAGT GAGGATGAGA ACCAAAGAGG ATGTGCCGGG AGCACCCCCC 
AGGAAGCTGG AGGTTGAGGC CATAAACTCC ACAGCAATCC GGGTCACCTG GAAGCCGCCC 
CTGCAGGGGA AGCAGCACGG CCAAATCCGG GGCTATCAAG TCATCTTCTC TCGGCTTGAA 
AACGGCGAAC CACGAGGCCA GCCTAACATC ATGGACGTTG CTTTGCCAGA GGCACAGTGG 
AATATTGAGG AGTCCACCGA GCATGAGGCC ATCATTGCCG GACTGCACTC TGAAACCACC 
TACTCGGTCA CCGTGGCAGC ATACACCACC AAAGGAGACG GAGCGCGCAG CAAAGCCAAA 
GTCATCACCA CGACGGGTGC AGTGCCAGGA AAGCCCACCA TGATGATCAG CACCACTATG 
GGCAACACAG CGCTGATTCA GTGGCAGCCA CCTAAGGAAA TGGTTGGGGA GCTGATGGGC 
TACCAGCTGC AGTACAAGCG CACTGACGAG GAAAACTACA CCTCCCGCGA GCTGAGAAAG 
ATCGATGACC ACTACACCGT CACCGGGCTG CACAAAGGTG CCACCTATGT CTTCCGCCTC 
AGTGCCCGCA ACCGCGCGGG CATCGGCGAA GCATACGTGA AAGAGATCAG CACCCCTGAA 
GACGTGCCGT CGGGCTTCCC GTTCAACCTG CAGGTCACGG GACTGACTCA GTCCAGCACC 
GAGCTGACCT GGGACCCTCC GCTGGTGGCC GAAAGGAACG GCAAGATCGT GCACTATGTG 
GTCGTTTACA GGGACATCAA CAGTCAGCAG AACAGCACCA ACCGGACGAG CGAAACGCAC 
ATGACCATCC AGGGCCTTAA TCCCGACACC ACCTACGATA TCCGGGTGCA GGCGTTCACC 
AGTAAGGGCG GAGGACCCCT CAGCCCTAGC ATCCAGAGCA GGACCATGTC GAACGATTTT 
CCCACCCTCA ACTTTGGTGT CAAAGCCGTC ATGAAAACAT CTGTCCTCCT CACCTGGGAT 
TTGCCACCGA ACTACAAATC CCAAGTGCCT TTCAAGATCC TGTACAACCA GCAAAGCGTG 
GAAGTGCAGG GATCCCTGAA GAGGAGGCTG ATCACGCAGC TGCAGCCAGA CACGGACTAC 
TCTTTTGTGC TGACAAGCAG GGGCAACATT GCTGGTGGTC TACAGCAGCA GGTGTCCATC 
CGCACCGCTC CCGACCTGAT CAACGCCAAG CCCAGCACAC ATCAGCCTCA GGGTAGCAAG 
ATGACAATCA ACCTGCCCAA GGTCCAGACC ACCACGCGCG TCAGGTGGTA CTACATCGTG 
GTGGTGCCGG TGTCCCAGTC AACGCAGGGG TGGAAGAATC CAGACGAGAT GGACCTGGAC 
GAGCTGTTCA AGTCAAGTGA AGGCCCCGTC CTGAGGCGCA GACGTCACCT GGATTCCTCC 
AAACCTTACA TCGCTGCCAA GTTGGCCTCG TTGCCGACCA CCTTCACCCT GGGTGATGAG 
AAGAGATACA ACGGCTTCTA CAACAAGCCC CTGAGCAGTC CGCAGGAGTA TCTCTGCTTT 
GTCCTGGCAG TACTCCGATC CGAAGGGGCA GACAGCACTA CTAACTATAT GACATTTTCA 
GCCAGTCCCT ACTCAGACCC GGTCCACGTG CGAAACAGCA TGGCTCAGGG GATGGAGCAG 
CCTGAAATGT TGTGGGTTAT GGGCCCTGTT TTGGCCGTGG TCCTGATTAT CATCATCGTC 
ATCGCCATCC TGCTCTTCAA GAAGAAGCGA GCGTCGCCCC TGCCCAAAGA TGAGCACTCG 
GGCGGGGTGA AGGACTCCCT GCTGGCCAAC TCCTCCGACC CTGTGGAGAT GAGAAGACTT 
AACTACCAGA CCCCAGGAAT GAGGGAGCAC CCGCCCATCC CTGTCGTCGA CCTGGCTGAC 
CACATAGAGC GTCTGAAGGC CAACGACGGC CTACGCTTTT CCCAGGAGTA CGAGTCGATT 
GATCCAGGGC AGCAGTTTAC CTGGGAGAAC TCCAACATGG AAGTCAACAA GCCAAAGAAT 
CGCTATGCAA ACGTCATTGC CTATGACCAC TCCAGAGTGG TTCTCACCTC TGTTGACGCC 
GTTCCAGGCA GTGACTACAT CAACGCCAAC TACATCGACG GCTACAGGAA GCAGAACGCC 
TACATCGCCA CGCAGGGGCC ACTTCCAGAG ACACTGAGCG ACTTCTGGAG GATGGTGTGG 
GAGCAAAGAT CCAACACCAT CGTCATGATG ACCAGACTGG AGGAGAAGTC ACGGGTGAAG 
TGTGACCAGT ACTGGCCGTC GCGAGGAACG GAGACCTATG GGATGATCCA GGTCACCATG 
CTGGACACCG TGGAGTTGGC CACTTACAGC GTCCGTACCT TTGCCCTCTA TAAGAATGGC 
TCCAGTGAGA AGAGAGAGGT GAGACAGTTC CAGTTCATGG CCTGGCCAGA CCACGGGGTG 
CCAGAGTACC CCACCCCAAT CCTGGCCTTC CTACGGCGGG TAAAGGCCTG CAATCCTCCA 
GACGCAGGGC CTATGGTGGT CCACTGCAGC GCGGGTGTGG GCAGGACCGG ATGCTTCATC 
GTGATAGATG CCATGCTGGA GCGCATGAAG CACGAGAAGT CCGTAGACAT CTACGGCCAC 
GTGACGTGCA TGCGCGCCCA GAGGAACTAC ATGGTCCAGA CCGAGGACCA GTACATCTTC 
ATTCACGAAG CCCTGCTGGA GGCCGCCACG TGCGGCAACA CCGAGGTGCC GGCGCGCAAC 
CTGTACGCCC ACATCCAGAA ACTCACCCAG CCCCCGCCCG GCGAGACGGT TACTGCCATG 
GAGCTGGAAT TCAAGAGACT GGCCAACTCC AAAGCCCACA CATCCCGCTT CATCAGCGCC 
AACTTGCCCT GCAACAAGTT CAAGAACCGC CTGGTCAACA TCATGCCTTT CGAGTCCACC 
CGTGTCTGCC TGCAGCCCAT CCGTGGCGTC GAGGGCTCCG ACTACATCAA TGCCAGCTGC 
ATCGACGGCT ACAGACAGCA GAAGGCCTAC CTGGCCACGC AGGGCCCACT GGCAGAGACC 
ACCGAGGACT TCTGGAGGAT GCTGTGGGAG CACAACTCCA CCATCGTCGT CATGCTCACC 
AAGCTACGAG AGATGGGCCG GGAGAAGTGC CATCAGTACT GGCCAGCAGA GCGTTCAGCC 
AGGTACCAGT ACTTTGTGGT GGATCCAATG GCTGAGTACA ACATGCCCCA GTACATCCTC 
AGAGAGTTCA AAGTCACAGA TGCCAGGGAC GGCCAGTCCA GAACAATCCG CCAATTCCAG 
TTCACTGACT GGCCCGAGCA AGGAGTGCCA AAAACCGGAG AGGGCTTCAT TGATTTTATC 
GGACAAGTGC ACAAAACCAA GGAACAGTTC GGACAGGACG GACCAATCAC TGTACACTGC 
AGTGCTGGGG TGGGCAGGAC CGGAGTCTTC ATCACCCTCA GCATCGTGCT GGAGAGGATG 
AGGTATGAAG GAGTTGTCGA CCTCTTCCAG ACAGTGAAGA CACTTCGCAC CCAGCGGCCT 
GCCATGGTGC AGACCGAGGA CCAGTACCAG TTGTGCTACA GGGCAGCATT GGAGTACCTG 
GGGAGCTTTG ACCACTATGC AACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104931439 ( XM_019263564.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019119109.1 Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X9, mRNA.

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CloneID	OLa84603
Clone ID Related Accession (Same CDS sequence)	XM_019263564.1
Accession Version	XM_019263564.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	receptor-type tyrosine-protein phosphatase F isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017607936.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTTGACATC 
AGCAGCAGCA ACGGACGCAT TAAACAGCTT CGTTCAGGTG GTACACCAAT TAGAGGAGCT 
CTGCAAATTG AGAACAGCGA GGAGTCCGAC CAAGGGAAAT ACGAGTGTGT TGCCATGAAC 
AGCGCTGGGA CCCGTTACTC CGCTCCTGCC AATCTCTACG TACGAGTGCG AAGAGTCCCA 
CCCAGATTCT CCATTCCGCC CACCAACCAA GAGGTGATGC CAGGCGGCAG CGTCAACCTG 
ACATGCGTGG CAGTGGGCTC GCCGATGCCC TACGTCAAGT GGACGATGGG CCTGGTGGAG 
CTGACCAAGG AGGAGGAGAT GCCGATGGGA CGCAACGTGC TGGAGGTCAA CAACATCCGC 
GAGTCGGCGA ACTACACCTG CGTGGCCATC TCCTCTCTGG GCATGATAGA AGCCACCGCT 
CAGGTCACCG TCAAAGCTTT GCCAAAGCCT CCCGCCTCCC TCATTGTGAC TGAGACCACA 
GCCACCAGTG TGACTCTCAC CTGGGACTCT GGAAACCCAG AACCTGTCTC CTACTACATG 
ATCCAATACA GAGCCAAGAC CTCGGACAAC GGCTTCCAGG AAGTGGAAGG TGTGGCAACT 
ACCCGTTACA GCATCGGTGG ACTTAGCCCC TACTCTGAGT ATGAGTTTCG TGTCATGGCT 
GTCAACAACA TCGGCCGGGG GCCACCGAGC GACCCCGTGG AGACACGCAC TGGTGAACAG 
GCCCCCTCCT CGCCACCTCT GCACGTCCAA GCGCGCATGC TGAGTGCCAG CACAATGCTG 
GTCCAGTGGG AACCGCCCGA GGAACCCAAC GGGCAAATCA GGGGCTACCG GGTCTACTAC 
AGCTCGGACT TGACGGCTCC GCTCAGCGCC TGGCAGAAGC ACAACACAGA CGACAGCAGC 
CTGACGACCA TCTCGGGCCT GACTCCTGAT ATCACCTACA GCCTCAGGGT GCTGGGCTTC 
ACCTCTGTAG GTGACGGGCC ACCTTCTGAC ATCCTGCAAG TCAAAACCCA ACAGGGAGTC 
CCTGCCCAGC CGTCCAGCTT TGAGGCCGAA GCTGAGCTGG ACAGTCGCAT CATGCTAACA 
TGGCTGTGGC CCGTCCAGGA CCCCATCACT AAGTATGAGC TGCAGTACTG GGAGGCCGGC 
TCAGACAACA AGTTACATGT GAGCTTCAAC CCAGCGGGCT CCTACGCTGT GGACGGTCTA 
AAGCCCGACA CCTTGTACCG TTTCTCTCTG GCAGCCCGTT CTGAGATGGG CTTAGGTGTC 
TACACCCAAC CCATCGAGGC TCGCACCGCC CAGTCCATGC CGGGAGCACC CCCCAGGAAG 
CTGGAGGTTG AGGCCATAAA CTCCACAGCA ATCCGGGTCA CCTGGAAGCC GCCCCTGCAG 
GGGAAGCAGC ACGGCCAAAT CCGGGGCTAT CAAGTCATCT TCTCTCGGCT TGAAAACGGC 
GAACCACGAG GCCAGCCTAA CATCATGGAC GTTGCTTTGC CAGAGGCACA GTGGAATATT 
GAGGAGTCCA CCGAGCATGA GGCCATCATT GCCGGACTGC ACTCTGAAAC CACCTACTCG 
GTCACCGTGG CAGCATACAC CACCAAAGGA GACGGAGCGC GCAGCAAAGC CAAAGTCATC 
ACCACGACGG GTGCAGTGCC AGGAAAGCCC ACCATGATGA TCAGCACCAC TATGGGCAAC 
ACAGCGCTGA TTCAGTGGCA GCCACCTAAG GAAATGGTTG GGGAGCTGAT GGGCTACCAG 
CTGCAGTACA AGCGCACTGA CGAGGAAAAC TACACCTCCC GCGAGCTGAG AAAGATCGAT 
GACCACTACA CCGTCACCGG GCTGCACAAA GGTGCCACCT ATGTCTTCCG CCTCAGTGCC 
CGCAACCGCG CGGGCATCGG CGAAGCATAC GTGAAAGAGA TCAGCACCCC TGAAGACGTG 
CCGTCGGGCT TCCCGTTCAA CCTGCAGGTC ACGGGACTGA CTCAGTCCAG CACCGAGCTG 
ACCTGGGACC CTCCGCTGGT GGCCGAAAGG AACGGCAAGA TCGTGCACTA TGTGGTCGTT 
TACAGGGACA TCAACAGTCA GCAGAACAGC ACCAACCGGA CGAGCGAAAC GCACATGACC 
ATCCAGGGCC TTAATCCCGA CACCACCTAC GATATCCGGG TGCAGGCGTT CACCAGTAAG 
GGCGGAGGAC CCCTCAGCCC TAGCATCCAG AGCAGGACCA TGTCGAACGA TTTTCCCACC 
CTCAACTTTG GTGTCAAAGC CGTCATGAAA ACATCTGTCC TCCTCACCTG GGATTTGCCA 
CCGAACTACA AATCCCAAGT GCCTTTCAAG ATCCTGTACA ACCAGCAAAG CGTGGAAGTG 
CAGGGATCCC TGAAGAGGAG GCTGATCACG CAGCTGCAGC CAGACACGGA CTACTCTTTT 
GTGCTGACAA GCAGGGGCAA CATTGCTGGT GGTCTACAGC AGCAGGTGTC CATCCGCACC 
GCTCCCGACC TGATCAACGC CAAGCCCAGC ACACATCAGC CTCAGGGTAG CAAGATGACA 
ATCAACCTGC CCAAGGTCCA GACCACCACG CGCGTCAGGT GGTACTACAT CGTGGTGGTG 
CCGGTGTCCC AGTCAACGCA GGGGTGGAAG AATCCAGACG AGATGGACCT GGACGAGCTG 
TTCAAGTCAA GTGAAGGCCC CGTCCTGAGG CGCAGACGTC ACCTGGATTC CTCCAAACCT 
TACATCGCTG CCAAGTTGGC CTCGTTGCCG ACCACCTTCA CCCTGGGTGA TGAGAAGAGA 
TACAACGGCT TCTACAACAA GCCCCTGAGC AGTCCGCAGG AGTATCTCTG CTTTGTCCTG 
GCAGTACTCC GATCCGAAGG GGCAGACAGC ACTACTAACT ATATGACATT TTCAGCCAGT 
CCCTACTCAG ACCCGGTCCA CGTGCGAAAC AGCATGGCTC AGGGGATGGA GCAGCCTGAA 
ATGTTGTGGG TTATGGGCCC TGTTTTGGCC GTGGTCCTGA TTATCATCAT CGTCATCGCC 
ATCCTGCTCT TCAAGAACAA ACAGGAAAGG AAGCGAGCGT CGCCCCTGCC CAAAGATGAG 
CACTCGGGCG GGGTGAAGGA CTCCCTGCTG GCCAACTCCT CCGACCCTGT GGAGATGAGA 
AGACTTAACT ACCAGACCCC AGGTCCCAGC TCTCATCGCT GCCCCAACAC TCCAAGAATG 
AGGGAGCACC CGCCCATCCC TGTCGTCGAC CTGGCTGACC ACATAGAGCG TCTGAAGGCC 
AACGACGGCC TACGCTTTTC CCAGGAGTAC GAGTCGATTG ATCCAGGGCA GCAGTTTACC 
TGGGAGAACT CCAACATGGA AGTCAACAAG CCAAAGAATC GCTATGCAAA CGTCATTGCC 
TATGACCACT CCAGAGTGGT TCTCACCTCT GTTGACGCCG TTCCAGGCAG TGACTACATC 
AACGCCAACT ACATCGACGG CTACAGGAAG CAGAACGCCT ACATCGCCAC GCAGGGGCCA 
CTTCCAGAGA CACTGAGCGA CTTCTGGAGG ATGGTGTGGG AGCAAAGATC CAACACCATC 
GTCATGATGA CCAGACTGGA GGAGAAGTCA CGGGTGAAGT GTGACCAGTA CTGGCCGTCG 
CGAGGAACGG AGACCTATGG GATGATCCAG GTCACCATGC TGGACACCGT GGAGTTGGCC 
ACTTACAGCG TCCGTACCTT TGCCCTCTAT AAGAATGGCT CCAGTGAGAA GAGAGAGGTG 
AGACAGTTCC AGTTCATGGC CTGGCCAGAC CACGGGGTGC CAGAGTACCC CACCCCAATC 
CTGGCCTTCC TACGGCGGGT AAAGGCCTGC AATCCTCCAG ACGCAGGGCC TATGGTGGTC 
CACTGCAGCG CGGGTGTGGG CAGGACCGGA TGCTTCATCG TGATAGATGC CATGCTGGAG 
CGCATGAAGC ACGAGAAGTC CGTAGACATC TACGGCCACG TGACGTGCAT GCGCGCCCAG 
AGGAACTACA TGGTCCAGAC CGAGGACCAG TACATCTTCA TTCACGAAGC CCTGCTGGAG 
GCCGCCACGT GCGGCAACAC CGAGGTGCCG GCGCGCAACC TGTACGCCCA CATCCAGAAA 
CTCACCCAGC CCCCGCCCGG CGAGACGGTT ACTGCCATGG AGCTGGAATT CAAGAGACTG 
GCCAACTCCA AAGCCCACAC ATCCCGCTTC ATCAGCGCCA ACTTGCCCTG CAACAAGTTC 
AAGAACCGCC TGGTCAACAT CATGCCTTTC GAGTCCACCC GTGTCTGCCT GCAGCCCATC 
CGTGGCGTCG AGGGCTCCGA CTACATCAAT GCCAGCTGCA TCGACGGCTA CAGACAGCAG 
AAGGCCTACC TGGCCACGCA GGGCCCACTG GCAGAGACCA CCGAGGACTT CTGGAGGATG 
CTGTGGGAGC ACAACTCCAC CATCGTCGTC ATGCTCACCA AGCTACGAGA GATGGGCCGG 
GAGAAGTGCC ATCAGTACTG GCCAGCAGAG CGTTCAGCCA GGTACCAGTA CTTTGTGGTG 
GATCCAATGG CTGAGTACAA CATGCCCCAG TACATCCTCA GAGAGTTCAA AGTCACAGAT 
GCCAGGGACG GCCAGTCCAG AACAATCCGC CAATTCCAGT TCACTGACTG GCCCGAGCAA 
GGAGTGCCAA AAACCGGAGA GGGCTTCATT GATTTTATCG GACAAGTGCA CAAAACCAAG 
GAACAGTTCG GACAGGACGG ACCAATCACT GTACACTGCA GTGCTGGGGT GGGCAGGACC 
GGAGTCTTCA TCACCCTCAG CATCGTGCTG GAGAGGATGA GGTATGAAGG AGTTGTCGAC 
CTCTTCCAGA CAGTGAAGAC ACTTCGCACC CAGCGGCCTG CCATGGTGCA GACCGAGGAC 
CAGTACCAGT TGTGCTACAG GGCAGCATTG GAGTACCTGG GGAGCTTTGA CCACTATGCA 
ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019119109.1
CDS	521..5986
Translation	MVPNIHLAVPFLPRGALLFLILLSCVVFSSLADSMPSFVKSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVEKTRTATMLCAASGNPDPEISWFKDMLPVDISSSNGRIKQLRSGGTPIRGALQIENSEESDQGKYECVAMNSAGTRYSAPANLYVRVRRVPPRFSIPPTNQEVMPGGSVNLTCVAVGSPMPYVKWTMGLVELTKEEEMPMGRNVLEVNNIRESANYTCVAISSLGMIEATAQVTVKALPKPPASLIVTETTATSVTLTWDSGNPEPVSYYMIQYRAKTSDNGFQEVEGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSDPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRVYYSSDLTAPLSAWQKHNTDDSSLTTISGLTPDITYSLRVLGFTSVGDGPPSDILQVKTQQGVPAQPSSFEAEAELDSRIMLTWLWPVQDPITKYELQYWEAGSDNKLHVSFNPAGSYAVDGLKPDTLYRFSLAARSEMGLGVYTQPIEARTAQSMPGAPPRKLEVEAINSTAIRVTWKPPLQGKQHGQIRGYQVIFSRLENGEPRGQPNIMDVALPEAQWNIEESTEHEAIIAGLHSETTYSVTVAAYTTKGDGARSKAKVITTTGAVPGKPTMMISTTMGNTALIQWQPPKEMVGELMGYQLQYKRTDEENYTSRELRKIDDHYTVTGLHKGATYVFRLSARNRAGIGEAYVKEISTPEDVPSGFPFNLQVTGLTQSSTELTWDPPLVAERNGKIVHYVVVYRDINSQQNSTNRTSETHMTIQGLNPDTTYDIRVQAFTSKGGGPLSPSIQSRTMSNDFPTLNFGVKAVMKTSVLLTWDLPPNYKSQVPFKILYNQQSVEVQGSLKRRLITQLQPDTDYSFVLTSRGNIAGGLQQQVSIRTAPDLINAKPSTHQPQGSKMTINLPKVQTTTRVRWYYIVVVPVSQSTQGWKNPDEMDLDELFKSSEGPVLRRRRHLDSSKPYIAAKLASLPTTFTLGDEKRYNGFYNKPLSSPQEYLCFVLAVLRSEGADSTTNYMTFSASPYSDPVHVRNSMAQGMEQPEMLWVMGPVLAVVLIIIIVIAILLFKNKQERKRASPLPKDEHSGGVKDSLLANSSDPVEMRRLNYQTPGPSSHRCPNTPRMREHPPIPVVDLADHIERLKANDGLRFSQEYESIDPGQQFTWENSNMEVNKPKNRYANVIAYDHSRVVLTSVDAVPGSDYINANYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRSNTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVTMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRAQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPPPGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYLATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_019263564.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019263564.1

ORF Insert Sequence:

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTTGACATC 
AGCAGCAGCA ACGGACGCAT TAAACAGCTT CGTTCAGGTG GTACACCAAT TAGAGGAGCT 
CTGCAAATTG AGAACAGCGA GGAGTCCGAC CAAGGGAAAT ACGAGTGTGT TGCCATGAAC 
AGCGCTGGGA CCCGTTACTC CGCTCCTGCC AATCTCTACG TACGAGTGCG AAGAGTCCCA 
CCCAGATTCT CCATTCCGCC CACCAACCAA GAGGTGATGC CAGGCGGCAG CGTCAACCTG 
ACATGCGTGG CAGTGGGCTC GCCGATGCCC TACGTCAAGT GGACGATGGG CCTGGTGGAG 
CTGACCAAGG AGGAGGAGAT GCCGATGGGA CGCAACGTGC TGGAGGTCAA CAACATCCGC 
GAGTCGGCGA ACTACACCTG CGTGGCCATC TCCTCTCTGG GCATGATAGA AGCCACCGCT 
CAGGTCACCG TCAAAGCTTT GCCAAAGCCT CCCGCCTCCC TCATTGTGAC TGAGACCACA 
GCCACCAGTG TGACTCTCAC CTGGGACTCT GGAAACCCAG AACCTGTCTC CTACTACATG 
ATCCAATACA GAGCCAAGAC CTCGGACAAC GGCTTCCAGG AAGTGGAAGG TGTGGCAACT 
ACCCGTTACA GCATCGGTGG ACTTAGCCCC TACTCTGAGT ATGAGTTTCG TGTCATGGCT 
GTCAACAACA TCGGCCGGGG GCCACCGAGC GACCCCGTGG AGACACGCAC TGGTGAACAG 
GCCCCCTCCT CGCCACCTCT GCACGTCCAA GCGCGCATGC TGAGTGCCAG CACAATGCTG 
GTCCAGTGGG AACCGCCCGA GGAACCCAAC GGGCAAATCA GGGGCTACCG GGTCTACTAC 
AGCTCGGACT TGACGGCTCC GCTCAGCGCC TGGCAGAAGC ACAACACAGA CGACAGCAGC 
CTGACGACCA TCTCGGGCCT GACTCCTGAT ATCACCTACA GCCTCAGGGT GCTGGGCTTC 
ACCTCTGTAG GTGACGGGCC ACCTTCTGAC ATCCTGCAAG TCAAAACCCA ACAGGGAGTC 
CCTGCCCAGC CGTCCAGCTT TGAGGCCGAA GCTGAGCTGG ACAGTCGCAT CATGCTAACA 
TGGCTGTGGC CCGTCCAGGA CCCCATCACT AAGTATGAGC TGCAGTACTG GGAGGCCGGC 
TCAGACAACA AGTTACATGT GAGCTTCAAC CCAGCGGGCT CCTACGCTGT GGACGGTCTA 
AAGCCCGACA CCTTGTACCG TTTCTCTCTG GCAGCCCGTT CTGAGATGGG CTTAGGTGTC 
TACACCCAAC CCATCGAGGC TCGCACCGCC CAGTCCATGC CGGGAGCACC CCCCAGGAAG 
CTGGAGGTTG AGGCCATAAA CTCCACAGCA ATCCGGGTCA CCTGGAAGCC GCCCCTGCAG 
GGGAAGCAGC ACGGCCAAAT CCGGGGCTAT CAAGTCATCT TCTCTCGGCT TGAAAACGGC 
GAACCACGAG GCCAGCCTAA CATCATGGAC GTTGCTTTGC CAGAGGCACA GTGGAATATT 
GAGGAGTCCA CCGAGCATGA GGCCATCATT GCCGGACTGC ACTCTGAAAC CACCTACTCG 
GTCACCGTGG CAGCATACAC CACCAAAGGA GACGGAGCGC GCAGCAAAGC CAAAGTCATC 
ACCACGACGG GTGCAGTGCC AGGAAAGCCC ACCATGATGA TCAGCACCAC TATGGGCAAC 
ACAGCGCTGA TTCAGTGGCA GCCACCTAAG GAAATGGTTG GGGAGCTGAT GGGCTACCAG 
CTGCAGTACA AGCGCACTGA CGAGGAAAAC TACACCTCCC GCGAGCTGAG AAAGATCGAT 
GACCACTACA CCGTCACCGG GCTGCACAAA GGTGCCACCT ATGTCTTCCG CCTCAGTGCC 
CGCAACCGCG CGGGCATCGG CGAAGCATAC GTGAAAGAGA TCAGCACCCC TGAAGACGTG 
CCGTCGGGCT TCCCGTTCAA CCTGCAGGTC ACGGGACTGA CTCAGTCCAG CACCGAGCTG 
ACCTGGGACC CTCCGCTGGT GGCCGAAAGG AACGGCAAGA TCGTGCACTA TGTGGTCGTT 
TACAGGGACA TCAACAGTCA GCAGAACAGC ACCAACCGGA CGAGCGAAAC GCACATGACC 
ATCCAGGGCC TTAATCCCGA CACCACCTAC GATATCCGGG TGCAGGCGTT CACCAGTAAG 
GGCGGAGGAC CCCTCAGCCC TAGCATCCAG AGCAGGACCA TGTCGAACGA TTTTCCCACC 
CTCAACTTTG GTGTCAAAGC CGTCATGAAA ACATCTGTCC TCCTCACCTG GGATTTGCCA 
CCGAACTACA AATCCCAAGT GCCTTTCAAG ATCCTGTACA ACCAGCAAAG CGTGGAAGTG 
CAGGGATCCC TGAAGAGGAG GCTGATCACG CAGCTGCAGC CAGACACGGA CTACTCTTTT 
GTGCTGACAA GCAGGGGCAA CATTGCTGGT GGTCTACAGC AGCAGGTGTC CATCCGCACC 
GCTCCCGACC TGATCAACGC CAAGCCCAGC ACACATCAGC CTCAGGGTAG CAAGATGACA 
ATCAACCTGC CCAAGGTCCA GACCACCACG CGCGTCAGGT GGTACTACAT CGTGGTGGTG 
CCGGTGTCCC AGTCAACGCA GGGGTGGAAG AATCCAGACG AGATGGACCT GGACGAGCTG 
TTCAAGTCAA GTGAAGGCCC CGTCCTGAGG CGCAGACGTC ACCTGGATTC CTCCAAACCT 
TACATCGCTG CCAAGTTGGC CTCGTTGCCG ACCACCTTCA CCCTGGGTGA TGAGAAGAGA 
TACAACGGCT TCTACAACAA GCCCCTGAGC AGTCCGCAGG AGTATCTCTG CTTTGTCCTG 
GCAGTACTCC GATCCGAAGG GGCAGACAGC ACTACTAACT ATATGACATT TTCAGCCAGT 
CCCTACTCAG ACCCGGTCCA CGTGCGAAAC AGCATGGCTC AGGGGATGGA GCAGCCTGAA 
ATGTTGTGGG TTATGGGCCC TGTTTTGGCC GTGGTCCTGA TTATCATCAT CGTCATCGCC 
ATCCTGCTCT TCAAGAACAA ACAGGAAAGG AAGCGAGCGT CGCCCCTGCC CAAAGATGAG 
CACTCGGGCG GGGTGAAGGA CTCCCTGCTG GCCAACTCCT CCGACCCTGT GGAGATGAGA 
AGACTTAACT ACCAGACCCC AGGTCCCAGC TCTCATCGCT GCCCCAACAC TCCAAGAATG 
AGGGAGCACC CGCCCATCCC TGTCGTCGAC CTGGCTGACC ACATAGAGCG TCTGAAGGCC 
AACGACGGCC TACGCTTTTC CCAGGAGTAC GAGTCGATTG ATCCAGGGCA GCAGTTTACC 
TGGGAGAACT CCAACATGGA AGTCAACAAG CCAAAGAATC GCTATGCAAA CGTCATTGCC 
TATGACCACT CCAGAGTGGT TCTCACCTCT GTTGACGCCG TTCCAGGCAG TGACTACATC 
AACGCCAACT ACATCGACGG CTACAGGAAG CAGAACGCCT ACATCGCCAC GCAGGGGCCA 
CTTCCAGAGA CACTGAGCGA CTTCTGGAGG ATGGTGTGGG AGCAAAGATC CAACACCATC 
GTCATGATGA CCAGACTGGA GGAGAAGTCA CGGGTGAAGT GTGACCAGTA CTGGCCGTCG 
CGAGGAACGG AGACCTATGG GATGATCCAG GTCACCATGC TGGACACCGT GGAGTTGGCC 
ACTTACAGCG TCCGTACCTT TGCCCTCTAT AAGAATGGCT CCAGTGAGAA GAGAGAGGTG 
AGACAGTTCC AGTTCATGGC CTGGCCAGAC CACGGGGTGC CAGAGTACCC CACCCCAATC 
CTGGCCTTCC TACGGCGGGT AAAGGCCTGC AATCCTCCAG ACGCAGGGCC TATGGTGGTC 
CACTGCAGCG CGGGTGTGGG CAGGACCGGA TGCTTCATCG TGATAGATGC CATGCTGGAG 
CGCATGAAGC ACGAGAAGTC CGTAGACATC TACGGCCACG TGACGTGCAT GCGCGCCCAG 
AGGAACTACA TGGTCCAGAC CGAGGACCAG TACATCTTCA TTCACGAAGC CCTGCTGGAG 
GCCGCCACGT GCGGCAACAC CGAGGTGCCG GCGCGCAACC TGTACGCCCA CATCCAGAAA 
CTCACCCAGC CCCCGCCCGG CGAGACGGTT ACTGCCATGG AGCTGGAATT CAAGAGACTG 
GCCAACTCCA AAGCCCACAC ATCCCGCTTC ATCAGCGCCA ACTTGCCCTG CAACAAGTTC 
AAGAACCGCC TGGTCAACAT CATGCCTTTC GAGTCCACCC GTGTCTGCCT GCAGCCCATC 
CGTGGCGTCG AGGGCTCCGA CTACATCAAT GCCAGCTGCA TCGACGGCTA CAGACAGCAG 
AAGGCCTACC TGGCCACGCA GGGCCCACTG GCAGAGACCA CCGAGGACTT CTGGAGGATG 
CTGTGGGAGC ACAACTCCAC CATCGTCGTC ATGCTCACCA AGCTACGAGA GATGGGCCGG 
GAGAAGTGCC ATCAGTACTG GCCAGCAGAG CGTTCAGCCA GGTACCAGTA CTTTGTGGTG 
GATCCAATGG CTGAGTACAA CATGCCCCAG TACATCCTCA GAGAGTTCAA AGTCACAGAT 
GCCAGGGACG GCCAGTCCAG AACAATCCGC CAATTCCAGT TCACTGACTG GCCCGAGCAA 
GGAGTGCCAA AAACCGGAGA GGGCTTCATT GATTTTATCG GACAAGTGCA CAAAACCAAG 
GAACAGTTCG GACAGGACGG ACCAATCACT GTACACTGCA GTGCTGGGGT GGGCAGGACC 
GGAGTCTTCA TCACCCTCAG CATCGTGCTG GAGAGGATGA GGTATGAAGG AGTTGTCGAC 
CTCTTCCAGA CAGTGAAGAC ACTTCGCACC CAGCGGCCTG CCATGGTGCA GACCGAGGAC 
CAGTACCAGT TGTGCTACAG GGCAGCATTG GAGTACCTGG GGAGCTTTGA CCACTATGCA 
ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104931439 ( XM_010746451.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010744753.2 Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X10, mRNA.

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CloneID	OLa84604
Clone ID Related Accession (Same CDS sequence)	XM_010746451.2
Accession Version	XM_010746451.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5448bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	receptor-type tyrosine-protein phosphatase F isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017607936.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 29, 2016 this sequence version replaced XM_010746451.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTTGACATC 
AGCAGCAGCA ACGGACGCAT TAAACAGCTT CGTTCAGGAG CTCTGCAAAT TGAGAACAGC 
GAGGAGTCCG ACCAAGGGAA ATACGAGTGT GTTGCCATGA ACAGCGCTGG GACCCGTTAC 
TCCGCTCCTG CCAATCTCTA CGTACGAGTG CGAAGAGTCC CACCCAGATT CTCCATTCCG 
CCCACCAACC AAGAGGTGAT GCCAGGCGGC AGCGTCAACC TGACATGCGT GGCAGTGGGC 
TCGCCGATGC CCTACGTCAA GTGGACGATG GGCCTGGTGG AGCTGACCAA GGAGGAGGAG 
ATGCCGATGG GACGCAACGT GCTGGAGGTC AACAACATCC GCGAGTCGGC GAACTACACC 
TGCGTGGCCA TCTCCTCTCT GGGCATGATA GAAGCCACCG CTCAGGTCAC CGTCAAAGCT 
TTGCCAAAGC CTCCCGCCTC CCTCATTGTG ACTGAGACCA CAGCCACCAG TGTGACTCTC 
ACCTGGGACT CTGGAAACCC AGAACCTGTC TCCTACTACA TGATCCAATA CAGAGCCAAG 
ACCTCGGACA ACGGCTTCCA GGAAGTGGAA GGTGTGGCAA CTACCCGTTA CAGCATCGGT 
GGACTTAGCC CCTACTCTGA GTATGAGTTT CGTGTCATGG CTGTCAACAA CATCGGCCGG 
GGGCCACCGA GCGACCCCGT GGAGACACGC ACTGGTGAAC AGGCCCCCTC CTCGCCACCT 
CTGCACGTCC AAGCGCGCAT GCTGAGTGCC AGCACAATGC TGGTCCAGTG GGAACCGCCC 
GAGGAACCCA ACGGGCAAAT CAGGGGCTAC CGGGTCTACT ACAGCTCGGA CTTGACGGCT 
CCGCTCAGCG CCTGGCAGAA GCACAACACA GACGACAGCA GCCTGACGAC CATCTCGGGC 
CTGACTCCTG ATATCACCTA CAGCCTCAGG GTGCTGGGCT TCACCTCTGT AGGTGACGGG 
CCACCTTCTG ACATCCTGCA AGTCAAAACC CAACAGGGAG TCCCTGCCCA GCCGTCCAGC 
TTTGAGGCCG AAGCTGAGCT GGACAGTCGC ATCATGCTAA CATGGCTGTG GCCCGTCCAG 
GACCCCATCA CTAAGTATGA GCTGCAGTAC TGGGAGGCCG GCTCAGACAA CAAGTTACAT 
GTGAGCTTCA ACCCAGCGGG CTCCTACGCT GTGGACGGTC TAAAGCCCGA CACCTTGTAC 
CGTTTCTCTC TGGCAGCCCG TTCTGAGATG GGCTTAGGTG TCTACACCCA ACCCATCGAG 
GCTCGCACCG CCCAGTCCAT GCCGGGAGCA CCCCCCAGGA AGCTGGAGGT TGAGGCCATA 
AACTCCACAG CAATCCGGGT CACCTGGAAG CCGCCCCTGC AGGGGAAGCA GCACGGCCAA 
ATCCGGGGCT ATCAAGTCAT CTTCTCTCGG CTTGAAAACG GCGAACCACG AGGCCAGCCT 
AACATCATGG ACGTTGCTTT GCCAGAGGCA CAGTGGAATA TTGAGGAGTC CACCGAGCAT 
GAGGCCATCA TTGCCGGACT GCACTCTGAA ACCACCTACT CGGTCACCGT GGCAGCATAC 
ACCACCAAAG GAGACGGAGC GCGCAGCAAA GCCAAAGTCA TCACCACGAC GGGTGCAGTG 
CCAGGAAAGC CCACCATGAT GATCAGCACC ACTATGGGCA ACACAGCGCT GATTCAGTGG 
CAGCCACCTA AGGAAATGGT TGGGGAGCTG ATGGGCTACC AGCTGCAGTA CAAGCGCACT 
GACGAGGAAA ACTACACCTC CCGCGAGCTG AGAAAGATCG ATGACCACTA CACCGTCACC 
GGGCTGCACA AAGGTGCCAC CTATGTCTTC CGCCTCAGTG CCCGCAACCG CGCGGGCATC 
GGCGAAGCAT ACGTGAAAGA GATCAGCACC CCTGAAGACG TGCCGTCGGG CTTCCCGTTC 
AACCTGCAGG TCACGGGACT GACTCAGTCC AGCACCGAGC TGACCTGGGA CCCTCCGCTG 
GTGGCCGAAA GGAACGGCAA GATCGTGCAC TATGTGGTCG TTTACAGGGA CATCAACAGT 
CAGCAGAACA GCACCAACCG GACGAGCGAA ACGCACATGA CCATCCAGGG CCTTAATCCC 
GACACCACCT ACGATATCCG GGTGCAGGCG TTCACCAGTA AGGGCGGAGG ACCCCTCAGC 
CCTAGCATCC AGAGCAGGAC CATGTCGAAC GATTTTCCCA CCCTCAACTT TGGTGTCAAA 
GCCGTCATGA AAACATCTGT CCTCCTCACC TGGGATTTGC CACCGAACTA CAAATCCCAA 
GTGCCTTTCA AGATCCTGTA CAACCAGCAA AGCGTGGAAG TGCAGGGATC CCTGAAGAGG 
AGGCTGATCA CGCAGCTGCA GCCAGACACG GACTACTCTT TTGTGCTGAC AAGCAGGGGC 
AACATTGCTG GTGGTCTACA GCAGCAGGTG TCCATCCGCA CCGCTCCCGA CCTGATCAAC 
GCCAAGCCCA GCACACATCA GCCTCAGGGT AGCAAGATGA CAATCAACCT GCCCAAGGTC 
CAGACCACCA CGCGCGTCAG GTGGTACTAC ATCGTGGTGG TGCCGGTGTC CCAGTCAACG 
CAGGGGTGGA AGAATCCAGA CGAGATGGAC CTGGACGAGC TGTTCAAGTC AAGTGAAGGC 
CCCGTCCTGA GGCGCAGACG TCACCTGGAT TCCTCCAAAC CTTACATCGC TGCCAAGTTG 
GCCTCGTTGC CGACCACCTT CACCCTGGGT GATGAGAAGA GATACAACGG CTTCTACAAC 
AAGCCCCTGA GCAGTCCGCA GGAGTATCTC TGCTTTGTCC TGGCAGTACT CCGATCCGAA 
GGGGCAGACA GCACTACTAA CTATATGACA TTTTCAGCCA GTCCCTACTC AGACCCGGTC 
CACGTGCGAA ACAGCATGGC TCAGGGGATG GAGCAGCCTG AAATGTTGTG GGTTATGGGC 
CCTGTTTTGG CCGTGGTCCT GATTATCATC ATCGTCATCG CCATCCTGCT CTTCAAGAAC 
AAACAGGAAA GGAAGCGAGC GTCGCCCCTG CCCAAAGATG AGCACTCGGG CGGGGTGAAG 
GACTCCCTGC TGGCCAACTC CTCCGACCCT GTGGAGATGA GAAGACTTAA CTACCAGACC 
CCAGGTCCCA GCTCTCATCG CTGCCCCAAC ACTCCAAGAA TGAGGGAGCA CCCGCCCATC 
CCTGTCGTCG ACCTGGCTGA CCACATAGAG CGTCTGAAGG CCAACGACGG CCTACGCTTT 
TCCCAGGAGT ACGAGTCGAT TGATCCAGGG CAGCAGTTTA CCTGGGAGAA CTCCAACATG 
GAAGTCAACA AGCCAAAGAA TCGCTATGCA AACGTCATTG CCTATGACCA CTCCAGAGTG 
GTTCTCACCT CTGTTGACGC CGTTCCAGGC AGTGACTACA TCAACGCCAA CTACATCGAC 
GGCTACAGGA AGCAGAACGC CTACATCGCC ACGCAGGGGC CACTTCCAGA GACACTGAGC 
GACTTCTGGA GGATGGTGTG GGAGCAAAGA TCCAACACCA TCGTCATGAT GACCAGACTG 
GAGGAGAAGT CACGGGTGAA GTGTGACCAG TACTGGCCGT CGCGAGGAAC GGAGACCTAT 
GGGATGATCC AGGTCACCAT GCTGGACACC GTGGAGTTGG CCACTTACAG CGTCCGTACC 
TTTGCCCTCT ATAAGAATGG CTCCAGTGAG AAGAGAGAGG TGAGACAGTT CCAGTTCATG 
GCCTGGCCAG ACCACGGGGT GCCAGAGTAC CCCACCCCAA TCCTGGCCTT CCTACGGCGG 
GTAAAGGCCT GCAATCCTCC AGACGCAGGG CCTATGGTGG TCCACTGCAG CGCGGGTGTG 
GGCAGGACCG GATGCTTCAT CGTGATAGAT GCCATGCTGG AGCGCATGAA GCACGAGAAG 
TCCGTAGACA TCTACGGCCA CGTGACGTGC ATGCGCGCCC AGAGGAACTA CATGGTCCAG 
ACCGAGGACC AGTACATCTT CATTCACGAA GCCCTGCTGG AGGCCGCCAC GTGCGGCAAC 
ACCGAGGTGC CGGCGCGCAA CCTGTACGCC CACATCCAGA AACTCACCCA GCCCCCGCCC 
GGCGAGACGG TTACTGCCAT GGAGCTGGAA TTCAAGAGAC TGGCCAACTC CAAAGCCCAC 
ACATCCCGCT TCATCAGCGC CAACTTGCCC TGCAACAAGT TCAAGAACCG CCTGGTCAAC 
ATCATGCCTT TCGAGTCCAC CCGTGTCTGC CTGCAGCCCA TCCGTGGCGT CGAGGGCTCC 
GACTACATCA ATGCCAGCTG CATCGACGGC TACAGACAGC AGAAGGCCTA CCTGGCCACG 
CAGGGCCCAC TGGCAGAGAC CACCGAGGAC TTCTGGAGGA TGCTGTGGGA GCACAACTCC 
ACCATCGTCG TCATGCTCAC CAAGCTACGA GAGATGGGCC GGGAGAAGTG CCATCAGTAC 
TGGCCAGCAG AGCGTTCAGC CAGGTACCAG TACTTTGTGG TGGATCCAAT GGCTGAGTAC 
AACATGCCCC AGTACATCCT CAGAGAGTTC AAAGTCACAG ATGCCAGGGA CGGCCAGTCC 
AGAACAATCC GCCAATTCCA GTTCACTGAC TGGCCCGAGC AAGGAGTGCC AAAAACCGGA 
GAGGGCTTCA TTGATTTTAT CGGACAAGTG CACAAAACCA AGGAACAGTT CGGACAGGAC 
GGACCAATCA CTGTACACTG CAGTGCTGGG GTGGGCAGGA CCGGAGTCTT CATCACCCTC 
AGCATCGTGC TGGAGAGGAT GAGGTATGAA GGAGTTGTCG ACCTCTTCCA GACAGTGAAG 
ACACTTCGCA CCCAGCGGCC TGCCATGGTG CAGACCGAGG ACCAGTACCA GTTGTGCTAC 
AGGGCAGCAT TGGAGTACCT GGGGAGCTTT GACCACTATG CAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010744753.2
CDS	521..5968
Translation	MVPNIHLAVPFLPRGALLFLILLSCVVFSSLADSMPSFVKSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVEKTRTATMLCAASGNPDPEISWFKDMLPVDISSSNGRIKQLRSGALQIENSEESDQGKYECVAMNSAGTRYSAPANLYVRVRRVPPRFSIPPTNQEVMPGGSVNLTCVAVGSPMPYVKWTMGLVELTKEEEMPMGRNVLEVNNIRESANYTCVAISSLGMIEATAQVTVKALPKPPASLIVTETTATSVTLTWDSGNPEPVSYYMIQYRAKTSDNGFQEVEGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSDPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRVYYSSDLTAPLSAWQKHNTDDSSLTTISGLTPDITYSLRVLGFTSVGDGPPSDILQVKTQQGVPAQPSSFEAEAELDSRIMLTWLWPVQDPITKYELQYWEAGSDNKLHVSFNPAGSYAVDGLKPDTLYRFSLAARSEMGLGVYTQPIEARTAQSMPGAPPRKLEVEAINSTAIRVTWKPPLQGKQHGQIRGYQVIFSRLENGEPRGQPNIMDVALPEAQWNIEESTEHEAIIAGLHSETTYSVTVAAYTTKGDGARSKAKVITTTGAVPGKPTMMISTTMGNTALIQWQPPKEMVGELMGYQLQYKRTDEENYTSRELRKIDDHYTVTGLHKGATYVFRLSARNRAGIGEAYVKEISTPEDVPSGFPFNLQVTGLTQSSTELTWDPPLVAERNGKIVHYVVVYRDINSQQNSTNRTSETHMTIQGLNPDTTYDIRVQAFTSKGGGPLSPSIQSRTMSNDFPTLNFGVKAVMKTSVLLTWDLPPNYKSQVPFKILYNQQSVEVQGSLKRRLITQLQPDTDYSFVLTSRGNIAGGLQQQVSIRTAPDLINAKPSTHQPQGSKMTINLPKVQTTTRVRWYYIVVVPVSQSTQGWKNPDEMDLDELFKSSEGPVLRRRRHLDSSKPYIAAKLASLPTTFTLGDEKRYNGFYNKPLSSPQEYLCFVLAVLRSEGADSTTNYMTFSASPYSDPVHVRNSMAQGMEQPEMLWVMGPVLAVVLIIIIVIAILLFKNKQERKRASPLPKDEHSGGVKDSLLANSSDPVEMRRLNYQTPGPSSHRCPNTPRMREHPPIPVVDLADHIERLKANDGLRFSQEYESIDPGQQFTWENSNMEVNKPKNRYANVIAYDHSRVVLTSVDAVPGSDYINANYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRSNTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVTMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRAQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPPPGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYLATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_010746451.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010746451.2

ORF Insert Sequence:

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTTGACATC 
AGCAGCAGCA ACGGACGCAT TAAACAGCTT CGTTCAGGAG CTCTGCAAAT TGAGAACAGC 
GAGGAGTCCG ACCAAGGGAA ATACGAGTGT GTTGCCATGA ACAGCGCTGG GACCCGTTAC 
TCCGCTCCTG CCAATCTCTA CGTACGAGTG CGAAGAGTCC CACCCAGATT CTCCATTCCG 
CCCACCAACC AAGAGGTGAT GCCAGGCGGC AGCGTCAACC TGACATGCGT GGCAGTGGGC 
TCGCCGATGC CCTACGTCAA GTGGACGATG GGCCTGGTGG AGCTGACCAA GGAGGAGGAG 
ATGCCGATGG GACGCAACGT GCTGGAGGTC AACAACATCC GCGAGTCGGC GAACTACACC 
TGCGTGGCCA TCTCCTCTCT GGGCATGATA GAAGCCACCG CTCAGGTCAC CGTCAAAGCT 
TTGCCAAAGC CTCCCGCCTC CCTCATTGTG ACTGAGACCA CAGCCACCAG TGTGACTCTC 
ACCTGGGACT CTGGAAACCC AGAACCTGTC TCCTACTACA TGATCCAATA CAGAGCCAAG 
ACCTCGGACA ACGGCTTCCA GGAAGTGGAA GGTGTGGCAA CTACCCGTTA CAGCATCGGT 
GGACTTAGCC CCTACTCTGA GTATGAGTTT CGTGTCATGG CTGTCAACAA CATCGGCCGG 
GGGCCACCGA GCGACCCCGT GGAGACACGC ACTGGTGAAC AGGCCCCCTC CTCGCCACCT 
CTGCACGTCC AAGCGCGCAT GCTGAGTGCC AGCACAATGC TGGTCCAGTG GGAACCGCCC 
GAGGAACCCA ACGGGCAAAT CAGGGGCTAC CGGGTCTACT ACAGCTCGGA CTTGACGGCT 
CCGCTCAGCG CCTGGCAGAA GCACAACACA GACGACAGCA GCCTGACGAC CATCTCGGGC 
CTGACTCCTG ATATCACCTA CAGCCTCAGG GTGCTGGGCT TCACCTCTGT AGGTGACGGG 
CCACCTTCTG ACATCCTGCA AGTCAAAACC CAACAGGGAG TCCCTGCCCA GCCGTCCAGC 
TTTGAGGCCG AAGCTGAGCT GGACAGTCGC ATCATGCTAA CATGGCTGTG GCCCGTCCAG 
GACCCCATCA CTAAGTATGA GCTGCAGTAC TGGGAGGCCG GCTCAGACAA CAAGTTACAT 
GTGAGCTTCA ACCCAGCGGG CTCCTACGCT GTGGACGGTC TAAAGCCCGA CACCTTGTAC 
CGTTTCTCTC TGGCAGCCCG TTCTGAGATG GGCTTAGGTG TCTACACCCA ACCCATCGAG 
GCTCGCACCG CCCAGTCCAT GCCGGGAGCA CCCCCCAGGA AGCTGGAGGT TGAGGCCATA 
AACTCCACAG CAATCCGGGT CACCTGGAAG CCGCCCCTGC AGGGGAAGCA GCACGGCCAA 
ATCCGGGGCT ATCAAGTCAT CTTCTCTCGG CTTGAAAACG GCGAACCACG AGGCCAGCCT 
AACATCATGG ACGTTGCTTT GCCAGAGGCA CAGTGGAATA TTGAGGAGTC CACCGAGCAT 
GAGGCCATCA TTGCCGGACT GCACTCTGAA ACCACCTACT CGGTCACCGT GGCAGCATAC 
ACCACCAAAG GAGACGGAGC GCGCAGCAAA GCCAAAGTCA TCACCACGAC GGGTGCAGTG 
CCAGGAAAGC CCACCATGAT GATCAGCACC ACTATGGGCA ACACAGCGCT GATTCAGTGG 
CAGCCACCTA AGGAAATGGT TGGGGAGCTG ATGGGCTACC AGCTGCAGTA CAAGCGCACT 
GACGAGGAAA ACTACACCTC CCGCGAGCTG AGAAAGATCG ATGACCACTA CACCGTCACC 
GGGCTGCACA AAGGTGCCAC CTATGTCTTC CGCCTCAGTG CCCGCAACCG CGCGGGCATC 
GGCGAAGCAT ACGTGAAAGA GATCAGCACC CCTGAAGACG TGCCGTCGGG CTTCCCGTTC 
AACCTGCAGG TCACGGGACT GACTCAGTCC AGCACCGAGC TGACCTGGGA CCCTCCGCTG 
GTGGCCGAAA GGAACGGCAA GATCGTGCAC TATGTGGTCG TTTACAGGGA CATCAACAGT 
CAGCAGAACA GCACCAACCG GACGAGCGAA ACGCACATGA CCATCCAGGG CCTTAATCCC 
GACACCACCT ACGATATCCG GGTGCAGGCG TTCACCAGTA AGGGCGGAGG ACCCCTCAGC 
CCTAGCATCC AGAGCAGGAC CATGTCGAAC GATTTTCCCA CCCTCAACTT TGGTGTCAAA 
GCCGTCATGA AAACATCTGT CCTCCTCACC TGGGATTTGC CACCGAACTA CAAATCCCAA 
GTGCCTTTCA AGATCCTGTA CAACCAGCAA AGCGTGGAAG TGCAGGGATC CCTGAAGAGG 
AGGCTGATCA CGCAGCTGCA GCCAGACACG GACTACTCTT TTGTGCTGAC AAGCAGGGGC 
AACATTGCTG GTGGTCTACA GCAGCAGGTG TCCATCCGCA CCGCTCCCGA CCTGATCAAC 
GCCAAGCCCA GCACACATCA GCCTCAGGGT AGCAAGATGA CAATCAACCT GCCCAAGGTC 
CAGACCACCA CGCGCGTCAG GTGGTACTAC ATCGTGGTGG TGCCGGTGTC CCAGTCAACG 
CAGGGGTGGA AGAATCCAGA CGAGATGGAC CTGGACGAGC TGTTCAAGTC AAGTGAAGGC 
CCCGTCCTGA GGCGCAGACG TCACCTGGAT TCCTCCAAAC CTTACATCGC TGCCAAGTTG 
GCCTCGTTGC CGACCACCTT CACCCTGGGT GATGAGAAGA GATACAACGG CTTCTACAAC 
AAGCCCCTGA GCAGTCCGCA GGAGTATCTC TGCTTTGTCC TGGCAGTACT CCGATCCGAA 
GGGGCAGACA GCACTACTAA CTATATGACA TTTTCAGCCA GTCCCTACTC AGACCCGGTC 
CACGTGCGAA ACAGCATGGC TCAGGGGATG GAGCAGCCTG AAATGTTGTG GGTTATGGGC 
CCTGTTTTGG CCGTGGTCCT GATTATCATC ATCGTCATCG CCATCCTGCT CTTCAAGAAC 
AAACAGGAAA GGAAGCGAGC GTCGCCCCTG CCCAAAGATG AGCACTCGGG CGGGGTGAAG 
GACTCCCTGC TGGCCAACTC CTCCGACCCT GTGGAGATGA GAAGACTTAA CTACCAGACC 
CCAGGTCCCA GCTCTCATCG CTGCCCCAAC ACTCCAAGAA TGAGGGAGCA CCCGCCCATC 
CCTGTCGTCG ACCTGGCTGA CCACATAGAG CGTCTGAAGG CCAACGACGG CCTACGCTTT 
TCCCAGGAGT ACGAGTCGAT TGATCCAGGG CAGCAGTTTA CCTGGGAGAA CTCCAACATG 
GAAGTCAACA AGCCAAAGAA TCGCTATGCA AACGTCATTG CCTATGACCA CTCCAGAGTG 
GTTCTCACCT CTGTTGACGC CGTTCCAGGC AGTGACTACA TCAACGCCAA CTACATCGAC 
GGCTACAGGA AGCAGAACGC CTACATCGCC ACGCAGGGGC CACTTCCAGA GACACTGAGC 
GACTTCTGGA GGATGGTGTG GGAGCAAAGA TCCAACACCA TCGTCATGAT GACCAGACTG 
GAGGAGAAGT CACGGGTGAA GTGTGACCAG TACTGGCCGT CGCGAGGAAC GGAGACCTAT 
GGGATGATCC AGGTCACCAT GCTGGACACC GTGGAGTTGG CCACTTACAG CGTCCGTACC 
TTTGCCCTCT ATAAGAATGG CTCCAGTGAG AAGAGAGAGG TGAGACAGTT CCAGTTCATG 
GCCTGGCCAG ACCACGGGGT GCCAGAGTAC CCCACCCCAA TCCTGGCCTT CCTACGGCGG 
GTAAAGGCCT GCAATCCTCC AGACGCAGGG CCTATGGTGG TCCACTGCAG CGCGGGTGTG 
GGCAGGACCG GATGCTTCAT CGTGATAGAT GCCATGCTGG AGCGCATGAA GCACGAGAAG 
TCCGTAGACA TCTACGGCCA CGTGACGTGC ATGCGCGCCC AGAGGAACTA CATGGTCCAG 
ACCGAGGACC AGTACATCTT CATTCACGAA GCCCTGCTGG AGGCCGCCAC GTGCGGCAAC 
ACCGAGGTGC CGGCGCGCAA CCTGTACGCC CACATCCAGA AACTCACCCA GCCCCCGCCC 
GGCGAGACGG TTACTGCCAT GGAGCTGGAA TTCAAGAGAC TGGCCAACTC CAAAGCCCAC 
ACATCCCGCT TCATCAGCGC CAACTTGCCC TGCAACAAGT TCAAGAACCG CCTGGTCAAC 
ATCATGCCTT TCGAGTCCAC CCGTGTCTGC CTGCAGCCCA TCCGTGGCGT CGAGGGCTCC 
GACTACATCA ATGCCAGCTG CATCGACGGC TACAGACAGC AGAAGGCCTA CCTGGCCACG 
CAGGGCCCAC TGGCAGAGAC CACCGAGGAC TTCTGGAGGA TGCTGTGGGA GCACAACTCC 
ACCATCGTCG TCATGCTCAC CAAGCTACGA GAGATGGGCC GGGAGAAGTG CCATCAGTAC 
TGGCCAGCAG AGCGTTCAGC CAGGTACCAG TACTTTGTGG TGGATCCAAT GGCTGAGTAC 
AACATGCCCC AGTACATCCT CAGAGAGTTC AAAGTCACAG ATGCCAGGGA CGGCCAGTCC 
AGAACAATCC GCCAATTCCA GTTCACTGAC TGGCCCGAGC AAGGAGTGCC AAAAACCGGA 
GAGGGCTTCA TTGATTTTAT CGGACAAGTG CACAAAACCA AGGAACAGTT CGGACAGGAC 
GGACCAATCA CTGTACACTG CAGTGCTGGG GTGGGCAGGA CCGGAGTCTT CATCACCCTC 
AGCATCGTGC TGGAGAGGAT GAGGTATGAA GGAGTTGTCG ACCTCTTCCA GACAGTGAAG 
ACACTTCGCA CCCAGCGGCC TGCCATGGTG CAGACCGAGG ACCAGTACCA GTTGTGCTAC 
AGGGCAGCAT TGGAGTACCT GGGGAGCTTT GACCACTATG CAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104931439 ( XM_010746452.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010744754.2 Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X11, mRNA.

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CloneID	OLa84605
Clone ID Related Accession (Same CDS sequence)	XM_010746452.2
Accession Version	XM_010746452.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	receptor-type tyrosine-protein phosphatase F isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017607936.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 29, 2016 this sequence version replaced XM_010746452.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTTGACATC 
AGCAGCAGCA ACGGACGCAT TAAACAGCTT CGTTCAGGAG CTCTGCAAAT TGAGAACAGC 
GAGGAGTCCG ACCAAGGGAA ATACGAGTGT GTTGCCATGA ACAGCGCTGG GACCCGTTAC 
TCCGCTCCTG CCAATCTCTA CGTACGAGTG CGAAGAGTCC CACCCAGATT CTCCATTCCG 
CCCACCAACC AAGAGGTGAT GCCAGGCGGC AGCGTCAACC TGACATGCGT GGCAGTGGGC 
TCGCCGATGC CCTACGTCAA GTGGACGATG GGCCTGGTGG AGCTGACCAA GGAGGAGGAG 
ATGCCGATGG GACGCAACGT GCTGGAGGTC AACAACATCC GCGAGTCGGC GAACTACACC 
TGCGTGGCCA TCTCCTCTCT GGGCATGATA GAAGCCACCG CTCAGGTCAC CGTCAAAGCT 
TTGCCAAAGC CTCCCGCCTC CCTCATTGTG ACTGAGACCA CAGCCACCAG TGTGACTCTC 
ACCTGGGACT CTGGAAACCC AGAACCTGTC TCCTACTACA TGATCCAATA CAGAGCCAAG 
ACCTCGGACA ACGGCTTCCA GGAAGTGGAA GGTGTGGCAA CTACCCGTTA CAGCATCGGT 
GGACTTAGCC CCTACTCTGA GTATGAGTTT CGTGTCATGG CTGTCAACAA CATCGGCCGG 
GGGCCACCGA GCGACCCCGT GGAGACACGC ACTGGTGAAC AGGCCCCCTC CTCGCCACCT 
CTGCACGTCC AAGCGCGCAT GCTGAGTGCC AGCACAATGC TGGTCCAGTG GGAACCGCCC 
GAGGAACCCA ACGGGCAAAT CAGGGGCTAC CGGGTCTACT ACAGCTCGGA CTTGACGGCT 
CCGCTCAGCG CCTGGCAGAA GCACAACACA GACGACAGCA GCCTGACGAC CATCTCGGGC 
CTGACTCCTG ATATCACCTA CAGCCTCAGG GTGCTGGGCT TCACCTCTGT AGGTGACGGG 
CCACCTTCTG ACATCCTGCA AGTCAAAACC CAACAGGGAG TCCCTGCCCA GCCGTCCAGC 
TTTGAGGCCG AAGCTGAGCT GGACAGTCGC ATCATGCTAA CATGGCTGTG GCCCGTCCAG 
GACCCCATCA CTAAGTATGA GCTGCAGTAC TGGGAGGCCG GCTCAGACAA CAAGTTACAT 
GTGAGCTTCA ACCCAGCGGG CTCCTACGCT GTGGACGGTC TAAAGCCCGA CACCTTGTAC 
CGTTTCTCTC TGGCAGCCCG TTCTGAGATG GGCTTAGGTG TCTACACCCA ACCCATCGAG 
GCTCGCACCG CCCAGTCCAT GCCGGGAGCA CCCCCCAGGA AGCTGGAGGT TGAGGCCATA 
AACTCCACAG CAATCCGGGT CACCTGGAAG CCGCCCCTGC AGGGGAAGCA GCACGGCCAA 
ATCCGGGGCT ATCAAGTCAT CTTCTCTCGG CTTGAAAACG GCGAACCACG AGGCCAGCCT 
AACATCATGG ACGTTGCTTT GCCAGAGGCA CAGGAGGCCA TCATTGCCGG ACTGCACTCT 
GAAACCACCT ACTCGGTCAC CGTGGCAGCA TACACCACCA AAGGAGACGG AGCGCGCAGC 
AAAGCCAAAG TCATCACCAC GACGGGTGCA GTGCCAGGAA AGCCCACCAT GATGATCAGC 
ACCACTATGG GCAACACAGC GCTGATTCAG TGGCAGCCAC CTAAGGAAAT GGTTGGGGAG 
CTGATGGGCT ACCAGCTGCA GTACAAGCGC ACTGACGAGG AAAACTACAC CTCCCGCGAG 
CTGAGAAAGA TCGATGACCA CTACACCGTC ACCGGGCTGC ACAAAGGTGC CACCTATGTC 
TTCCGCCTCA GTGCCCGCAA CCGCGCGGGC ATCGGCGAAG CATACGTGAA AGAGATCAGC 
ACCCCTGAAG ACGTGCCGTC GGGCTTCCCG TTCAACCTGC AGGTCACGGG ACTGACTCAG 
TCCAGCACCG AGCTGACCTG GGACCCTCCG CTGGTGGCCG AAAGGAACGG CAAGATCGTG 
CACTATGTGG TCGTTTACAG GGACATCAAC AGTCAGCAGA ACAGCACCAA CCGGACGAGC 
GAAACGCACA TGACCATCCA GGGCCTTAAT CCCGACACCA CCTACGATAT CCGGGTGCAG 
GCGTTCACCA GTAAGGGCGG AGGACCCCTC AGCCCTAGCA TCCAGAGCAG GACCATGTCG 
AACGATTTTC CCACCCTCAA CTTTGGTGTC AAAGCCGTCA TGAAAACATC TGTCCTCCTC 
ACCTGGGATT TGCCACCGAA CTACAAATCC CAAGTGCCTT TCAAGATCCT GTACAACCAG 
CAAAGCGTGG AAGTGCAGGG ATCCCTGAAG AGGAGGCTGA TCACGCAGCT GCAGCCAGAC 
ACGGACTACT CTTTTGTGCT GACAAGCAGG GGCAACATTG CTGGTGGTCT ACAGCAGCAG 
GTGTCCATCC GCACCGCTCC CGACCTGATC AACGCCAAGC CCAGCACACA TCAGCCTCAG 
GGTAGCAAGA TGACAATCAA CCTGCCCAAG GTCCAGACCA CCACGCGCGT CAGGTGGTAC 
TACATCGTGG TGGTGCCGGT GTCCCAGTCA ACGCAGGGGT GGAAGAATCC AGACGAGATG 
GACCTGGACG AGCTGTTCAA GTCAAGTGAA GGCCCCGTCC TGAGGCGCAG ACGTCACCTG 
GATTCCTCCA AACCTTACAT CGCTGCCAAG TTGGCCTCGT TGCCGACCAC CTTCACCCTG 
GGTGATGAGA AGAGATACAA CGGCTTCTAC AACAAGCCCC TGAGCAGTCC GCAGGAGTAT 
CTCTGCTTTG TCCTGGCAGT ACTCCGATCC GAAGGGGCAG ACAGCACTAC TAACTATATG 
ACATTTTCAG CCAGTCCCTA CTCAGACCCG GTCCACGTGC GAAACAGCAT GGCTCAGGGG 
ATGGAGCAGC CTGAAATGTT GTGGGTTATG GGCCCTGTTT TGGCCGTGGT CCTGATTATC 
ATCATCGTCA TCGCCATCCT GCTCTTCAAG AACAAACAGG AAAGGAAGCG AGCGTCGCCC 
CTGCCCAAAG ATGAGCACTC GGGCGGGGTG AAGGACTCCC TGCTGGCCAA CTCCTCCGAC 
CCTGTGGAGA TGAGAAGACT TAACTACCAG ACCCCAGGTC CCAGCTCTCA TCGCTGCCCC 
AACACTCCAA GAATGAGGGA GCACCCGCCC ATCCCTGTCG TCGACCTGGC TGACCACATA 
GAGCGTCTGA AGGCCAACGA CGGCCTACGC TTTTCCCAGG AGTACGAGTC GATTGATCCA 
GGGCAGCAGT TTACCTGGGA GAACTCCAAC ATGGAAGTCA ACAAGCCAAA GAATCGCTAT 
GCAAACGTCA TTGCCTATGA CCACTCCAGA GTGGTTCTCA CCTCTGTTGA CGCCGTTCCA 
GGCAGTGACT ACATCAACGC CAACTACATC GACGGCTACA GGAAGCAGAA CGCCTACATC 
GCCACGCAGG GGCCACTTCC AGAGACACTG AGCGACTTCT GGAGGATGGT GTGGGAGCAA 
AGATCCAACA CCATCGTCAT GATGACCAGA CTGGAGGAGA AGTCACGGGT GAAGTGTGAC 
CAGTACTGGC CGTCGCGAGG AACGGAGACC TATGGGATGA TCCAGGTCAC CATGCTGGAC 
ACCGTGGAGT TGGCCACTTA CAGCGTCCGT ACCTTTGCCC TCTATAAGAA TGGCTCCAGT 
GAGAAGAGAG AGGTGAGACA GTTCCAGTTC ATGGCCTGGC CAGACCACGG GGTGCCAGAG 
TACCCCACCC CAATCCTGGC CTTCCTACGG CGGGTAAAGG CCTGCAATCC TCCAGACGCA 
GGGCCTATGG TGGTCCACTG CAGCGCGGGT GTGGGCAGGA CCGGATGCTT CATCGTGATA 
GATGCCATGC TGGAGCGCAT GAAGCACGAG AAGTCCGTAG ACATCTACGG CCACGTGACG 
TGCATGCGCG CCCAGAGGAA CTACATGGTC CAGACCGAGG ACCAGTACAT CTTCATTCAC 
GAAGCCCTGC TGGAGGCCGC CACGTGCGGC AACACCGAGG TGCCGGCGCG CAACCTGTAC 
GCCCACATCC AGAAACTCAC CCAGCCCCCG CCCGGCGAGA CGGTTACTGC CATGGAGCTG 
GAATTCAAGA GACTGGCCAA CTCCAAAGCC CACACATCCC GCTTCATCAG CGCCAACTTG 
CCCTGCAACA AGTTCAAGAA CCGCCTGGTC AACATCATGC CTTTCGAGTC CACCCGTGTC 
TGCCTGCAGC CCATCCGTGG CGTCGAGGGC TCCGACTACA TCAATGCCAG CTGCATCGAC 
GGCTACAGAC AGCAGAAGGC CTACCTGGCC ACGCAGGGCC CACTGGCAGA GACCACCGAG 
GACTTCTGGA GGATGCTGTG GGAGCACAAC TCCACCATCG TCGTCATGCT CACCAAGCTA 
CGAGAGATGG GCCGGGAGAA GTGCCATCAG TACTGGCCAG CAGAGCGTTC AGCCAGGTAC 
CAGTACTTTG TGGTGGATCC AATGGCTGAG TACAACATGC CCCAGTACAT CCTCAGAGAG 
TTCAAAGTCA CAGATGCCAG GGACGGCCAG TCCAGAACAA TCCGCCAATT CCAGTTCACT 
GACTGGCCCG AGCAAGGAGT GCCAAAAACC GGAGAGGGCT TCATTGATTT TATCGGACAA 
GTGCACAAAA CCAAGGAACA GTTCGGACAG GACGGACCAA TCACTGTACA CTGCAGTGCT 
GGGGTGGGCA GGACCGGAGT CTTCATCACC CTCAGCATCG TGCTGGAGAG GATGAGGTAT 
GAAGGAGTTG TCGACCTCTT CCAGACAGTG AAGACACTTC GCACCCAGCG GCCTGCCATG 
GTGCAGACCG AGGACCAGTA CCAGTTGTGC TACAGGGCAG CATTGGAGTA CCTGGGGAGC 
TTTGACCACT ATGCAACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010744754.2
CDS	520..5940
Translation	MVPNIHLAVPFLPRGALLFLILLSCVVFSSLADSMPSFVKSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVEKTRTATMLCAASGNPDPEISWFKDMLPVDISSSNGRIKQLRSGALQIENSEESDQGKYECVAMNSAGTRYSAPANLYVRVRRVPPRFSIPPTNQEVMPGGSVNLTCVAVGSPMPYVKWTMGLVELTKEEEMPMGRNVLEVNNIRESANYTCVAISSLGMIEATAQVTVKALPKPPASLIVTETTATSVTLTWDSGNPEPVSYYMIQYRAKTSDNGFQEVEGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSDPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRVYYSSDLTAPLSAWQKHNTDDSSLTTISGLTPDITYSLRVLGFTSVGDGPPSDILQVKTQQGVPAQPSSFEAEAELDSRIMLTWLWPVQDPITKYELQYWEAGSDNKLHVSFNPAGSYAVDGLKPDTLYRFSLAARSEMGLGVYTQPIEARTAQSMPGAPPRKLEVEAINSTAIRVTWKPPLQGKQHGQIRGYQVIFSRLENGEPRGQPNIMDVALPEAQEAIIAGLHSETTYSVTVAAYTTKGDGARSKAKVITTTGAVPGKPTMMISTTMGNTALIQWQPPKEMVGELMGYQLQYKRTDEENYTSRELRKIDDHYTVTGLHKGATYVFRLSARNRAGIGEAYVKEISTPEDVPSGFPFNLQVTGLTQSSTELTWDPPLVAERNGKIVHYVVVYRDINSQQNSTNRTSETHMTIQGLNPDTTYDIRVQAFTSKGGGPLSPSIQSRTMSNDFPTLNFGVKAVMKTSVLLTWDLPPNYKSQVPFKILYNQQSVEVQGSLKRRLITQLQPDTDYSFVLTSRGNIAGGLQQQVSIRTAPDLINAKPSTHQPQGSKMTINLPKVQTTTRVRWYYIVVVPVSQSTQGWKNPDEMDLDELFKSSEGPVLRRRRHLDSSKPYIAAKLASLPTTFTLGDEKRYNGFYNKPLSSPQEYLCFVLAVLRSEGADSTTNYMTFSASPYSDPVHVRNSMAQGMEQPEMLWVMGPVLAVVLIIIIVIAILLFKNKQERKRASPLPKDEHSGGVKDSLLANSSDPVEMRRLNYQTPGPSSHRCPNTPRMREHPPIPVVDLADHIERLKANDGLRFSQEYESIDPGQQFTWENSNMEVNKPKNRYANVIAYDHSRVVLTSVDAVPGSDYINANYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRSNTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVTMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRAQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPPPGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYLATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_010746452.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010746452.2

ORF Insert Sequence:

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTTGACATC 
AGCAGCAGCA ACGGACGCAT TAAACAGCTT CGTTCAGGAG CTCTGCAAAT TGAGAACAGC 
GAGGAGTCCG ACCAAGGGAA ATACGAGTGT GTTGCCATGA ACAGCGCTGG GACCCGTTAC 
TCCGCTCCTG CCAATCTCTA CGTACGAGTG CGAAGAGTCC CACCCAGATT CTCCATTCCG 
CCCACCAACC AAGAGGTGAT GCCAGGCGGC AGCGTCAACC TGACATGCGT GGCAGTGGGC 
TCGCCGATGC CCTACGTCAA GTGGACGATG GGCCTGGTGG AGCTGACCAA GGAGGAGGAG 
ATGCCGATGG GACGCAACGT GCTGGAGGTC AACAACATCC GCGAGTCGGC GAACTACACC 
TGCGTGGCCA TCTCCTCTCT GGGCATGATA GAAGCCACCG CTCAGGTCAC CGTCAAAGCT 
TTGCCAAAGC CTCCCGCCTC CCTCATTGTG ACTGAGACCA CAGCCACCAG TGTGACTCTC 
ACCTGGGACT CTGGAAACCC AGAACCTGTC TCCTACTACA TGATCCAATA CAGAGCCAAG 
ACCTCGGACA ACGGCTTCCA GGAAGTGGAA GGTGTGGCAA CTACCCGTTA CAGCATCGGT 
GGACTTAGCC CCTACTCTGA GTATGAGTTT CGTGTCATGG CTGTCAACAA CATCGGCCGG 
GGGCCACCGA GCGACCCCGT GGAGACACGC ACTGGTGAAC AGGCCCCCTC CTCGCCACCT 
CTGCACGTCC AAGCGCGCAT GCTGAGTGCC AGCACAATGC TGGTCCAGTG GGAACCGCCC 
GAGGAACCCA ACGGGCAAAT CAGGGGCTAC CGGGTCTACT ACAGCTCGGA CTTGACGGCT 
CCGCTCAGCG CCTGGCAGAA GCACAACACA GACGACAGCA GCCTGACGAC CATCTCGGGC 
CTGACTCCTG ATATCACCTA CAGCCTCAGG GTGCTGGGCT TCACCTCTGT AGGTGACGGG 
CCACCTTCTG ACATCCTGCA AGTCAAAACC CAACAGGGAG TCCCTGCCCA GCCGTCCAGC 
TTTGAGGCCG AAGCTGAGCT GGACAGTCGC ATCATGCTAA CATGGCTGTG GCCCGTCCAG 
GACCCCATCA CTAAGTATGA GCTGCAGTAC TGGGAGGCCG GCTCAGACAA CAAGTTACAT 
GTGAGCTTCA ACCCAGCGGG CTCCTACGCT GTGGACGGTC TAAAGCCCGA CACCTTGTAC 
CGTTTCTCTC TGGCAGCCCG TTCTGAGATG GGCTTAGGTG TCTACACCCA ACCCATCGAG 
GCTCGCACCG CCCAGTCCAT GCCGGGAGCA CCCCCCAGGA AGCTGGAGGT TGAGGCCATA 
AACTCCACAG CAATCCGGGT CACCTGGAAG CCGCCCCTGC AGGGGAAGCA GCACGGCCAA 
ATCCGGGGCT ATCAAGTCAT CTTCTCTCGG CTTGAAAACG GCGAACCACG AGGCCAGCCT 
AACATCATGG ACGTTGCTTT GCCAGAGGCA CAGGAGGCCA TCATTGCCGG ACTGCACTCT 
GAAACCACCT ACTCGGTCAC CGTGGCAGCA TACACCACCA AAGGAGACGG AGCGCGCAGC 
AAAGCCAAAG TCATCACCAC GACGGGTGCA GTGCCAGGAA AGCCCACCAT GATGATCAGC 
ACCACTATGG GCAACACAGC GCTGATTCAG TGGCAGCCAC CTAAGGAAAT GGTTGGGGAG 
CTGATGGGCT ACCAGCTGCA GTACAAGCGC ACTGACGAGG AAAACTACAC CTCCCGCGAG 
CTGAGAAAGA TCGATGACCA CTACACCGTC ACCGGGCTGC ACAAAGGTGC CACCTATGTC 
TTCCGCCTCA GTGCCCGCAA CCGCGCGGGC ATCGGCGAAG CATACGTGAA AGAGATCAGC 
ACCCCTGAAG ACGTGCCGTC GGGCTTCCCG TTCAACCTGC AGGTCACGGG ACTGACTCAG 
TCCAGCACCG AGCTGACCTG GGACCCTCCG CTGGTGGCCG AAAGGAACGG CAAGATCGTG 
CACTATGTGG TCGTTTACAG GGACATCAAC AGTCAGCAGA ACAGCACCAA CCGGACGAGC 
GAAACGCACA TGACCATCCA GGGCCTTAAT CCCGACACCA CCTACGATAT CCGGGTGCAG 
GCGTTCACCA GTAAGGGCGG AGGACCCCTC AGCCCTAGCA TCCAGAGCAG GACCATGTCG 
AACGATTTTC CCACCCTCAA CTTTGGTGTC AAAGCCGTCA TGAAAACATC TGTCCTCCTC 
ACCTGGGATT TGCCACCGAA CTACAAATCC CAAGTGCCTT TCAAGATCCT GTACAACCAG 
CAAAGCGTGG AAGTGCAGGG ATCCCTGAAG AGGAGGCTGA TCACGCAGCT GCAGCCAGAC 
ACGGACTACT CTTTTGTGCT GACAAGCAGG GGCAACATTG CTGGTGGTCT ACAGCAGCAG 
GTGTCCATCC GCACCGCTCC CGACCTGATC AACGCCAAGC CCAGCACACA TCAGCCTCAG 
GGTAGCAAGA TGACAATCAA CCTGCCCAAG GTCCAGACCA CCACGCGCGT CAGGTGGTAC 
TACATCGTGG TGGTGCCGGT GTCCCAGTCA ACGCAGGGGT GGAAGAATCC AGACGAGATG 
GACCTGGACG AGCTGTTCAA GTCAAGTGAA GGCCCCGTCC TGAGGCGCAG ACGTCACCTG 
GATTCCTCCA AACCTTACAT CGCTGCCAAG TTGGCCTCGT TGCCGACCAC CTTCACCCTG 
GGTGATGAGA AGAGATACAA CGGCTTCTAC AACAAGCCCC TGAGCAGTCC GCAGGAGTAT 
CTCTGCTTTG TCCTGGCAGT ACTCCGATCC GAAGGGGCAG ACAGCACTAC TAACTATATG 
ACATTTTCAG CCAGTCCCTA CTCAGACCCG GTCCACGTGC GAAACAGCAT GGCTCAGGGG 
ATGGAGCAGC CTGAAATGTT GTGGGTTATG GGCCCTGTTT TGGCCGTGGT CCTGATTATC 
ATCATCGTCA TCGCCATCCT GCTCTTCAAG AACAAACAGG AAAGGAAGCG AGCGTCGCCC 
CTGCCCAAAG ATGAGCACTC GGGCGGGGTG AAGGACTCCC TGCTGGCCAA CTCCTCCGAC 
CCTGTGGAGA TGAGAAGACT TAACTACCAG ACCCCAGGTC CCAGCTCTCA TCGCTGCCCC 
AACACTCCAA GAATGAGGGA GCACCCGCCC ATCCCTGTCG TCGACCTGGC TGACCACATA 
GAGCGTCTGA AGGCCAACGA CGGCCTACGC TTTTCCCAGG AGTACGAGTC GATTGATCCA 
GGGCAGCAGT TTACCTGGGA GAACTCCAAC ATGGAAGTCA ACAAGCCAAA GAATCGCTAT 
GCAAACGTCA TTGCCTATGA CCACTCCAGA GTGGTTCTCA CCTCTGTTGA CGCCGTTCCA 
GGCAGTGACT ACATCAACGC CAACTACATC GACGGCTACA GGAAGCAGAA CGCCTACATC 
GCCACGCAGG GGCCACTTCC AGAGACACTG AGCGACTTCT GGAGGATGGT GTGGGAGCAA 
AGATCCAACA CCATCGTCAT GATGACCAGA CTGGAGGAGA AGTCACGGGT GAAGTGTGAC 
CAGTACTGGC CGTCGCGAGG AACGGAGACC TATGGGATGA TCCAGGTCAC CATGCTGGAC 
ACCGTGGAGT TGGCCACTTA CAGCGTCCGT ACCTTTGCCC TCTATAAGAA TGGCTCCAGT 
GAGAAGAGAG AGGTGAGACA GTTCCAGTTC ATGGCCTGGC CAGACCACGG GGTGCCAGAG 
TACCCCACCC CAATCCTGGC CTTCCTACGG CGGGTAAAGG CCTGCAATCC TCCAGACGCA 
GGGCCTATGG TGGTCCACTG CAGCGCGGGT GTGGGCAGGA CCGGATGCTT CATCGTGATA 
GATGCCATGC TGGAGCGCAT GAAGCACGAG AAGTCCGTAG ACATCTACGG CCACGTGACG 
TGCATGCGCG CCCAGAGGAA CTACATGGTC CAGACCGAGG ACCAGTACAT CTTCATTCAC 
GAAGCCCTGC TGGAGGCCGC CACGTGCGGC AACACCGAGG TGCCGGCGCG CAACCTGTAC 
GCCCACATCC AGAAACTCAC CCAGCCCCCG CCCGGCGAGA CGGTTACTGC CATGGAGCTG 
GAATTCAAGA GACTGGCCAA CTCCAAAGCC CACACATCCC GCTTCATCAG CGCCAACTTG 
CCCTGCAACA AGTTCAAGAA CCGCCTGGTC AACATCATGC CTTTCGAGTC CACCCGTGTC 
TGCCTGCAGC CCATCCGTGG CGTCGAGGGC TCCGACTACA TCAATGCCAG CTGCATCGAC 
GGCTACAGAC AGCAGAAGGC CTACCTGGCC ACGCAGGGCC CACTGGCAGA GACCACCGAG 
GACTTCTGGA GGATGCTGTG GGAGCACAAC TCCACCATCG TCGTCATGCT CACCAAGCTA 
CGAGAGATGG GCCGGGAGAA GTGCCATCAG TACTGGCCAG CAGAGCGTTC AGCCAGGTAC 
CAGTACTTTG TGGTGGATCC AATGGCTGAG TACAACATGC CCCAGTACAT CCTCAGAGAG 
TTCAAAGTCA CAGATGCCAG GGACGGCCAG TCCAGAACAA TCCGCCAATT CCAGTTCACT 
GACTGGCCCG AGCAAGGAGT GCCAAAAACC GGAGAGGGCT TCATTGATTT TATCGGACAA 
GTGCACAAAA CCAAGGAACA GTTCGGACAG GACGGACCAA TCACTGTACA CTGCAGTGCT 
GGGGTGGGCA GGACCGGAGT CTTCATCACC CTCAGCATCG TGCTGGAGAG GATGAGGTAT 
GAAGGAGTTG TCGACCTCTT CCAGACAGTG AAGACACTTC GCACCCAGCG GCCTGCCATG 
GTGCAGACCG AGGACCAGTA CCAGTTGTGC TACAGGGCAG CATTGGAGTA CCTGGGGAGC 
TTTGACCACT ATGCAACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104931439 ( XM_010746450.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010744752.2 Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X12, mRNA.

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CloneID	OLa84606
Clone ID Related Accession (Same CDS sequence)	XM_010746450.2
Accession Version	XM_010746450.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5709bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	receptor-type tyrosine-protein phosphatase F isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017607936.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 29, 2016 this sequence version replaced XM_010746450.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTTGACATC 
AGCAGCAGCA ACGGACGCAT TAAACAGCTT CGTTCAGGAG CTCTGCAAAT TGAGAACAGC 
GAGGAGTCCG ACCAAGGGAA ATACGAGTGT GTTGCCATGA ACAGCGCTGG GACCCGTTAC 
TCCGCTCCTG CCAATCTCTA CGTACGAGTG CGAAGAGTCC CACCCAGATT CTCCATTCCG 
CCCACCAACC AAGAGGTGAT GCCAGGCGGC AGCGTCAACC TGACATGCGT GGCAGTGGGC 
TCGCCGATGC CCTACGTCAA GTGGACGATG GGCCTGGTGG AGCTGACCAA GGAGGAGGAG 
ATGCCGATGG GACGCAACGT GCTGGAGGTC AACAACATCC GCGAGTCGGC GAACTACACC 
TGCGTGGCCA TCTCCTCTCT GGGCATGATA GAAGCCACCG CTCAGGTCAC CGTCAAAGCT 
TTGCCAAAGC CTCCCGCCTC CCTCATTGTG ACTGAGACCA CAGCCACCAG TGTGACTCTC 
ACCTGGGACT CTGGAAACCC AGAACCTGTC TCCTACTACA TGATCCAATA CAGAGCCAAG 
ACCTCGGACA ACGGCTTCCA GGAAGTGGAA GGTGTGGCAA CTACCCGTTA CAGCATCGGT 
GGACTTAGCC CCTACTCTGA GTATGAGTTT CGTGTCATGG CTGTCAACAA CATCGGCCGG 
GGGCCACCGA GCGACCCCGT GGAGACACGC ACTGGTGAAC AGGCCCCCTC CTCGCCACCT 
CTGCACGTCC AAGCGCGCAT GCTGAGTGCC AGCACAATGC TGGTCCAGTG GGAACCGCCC 
GAGGAACCCA ACGGGCAAAT CAGGGGCTAC CGGGTCTACT ACAGCTCGGA CTTGACGGCT 
CCGCTCAGCG CCTGGCAGAA GCACAACACA GACGACAGCA GCCTGACGAC CATCTCGGGC 
CTGACTCCTG ATATCACCTA CAGCCTCAGG GTGCTGGGCT TCACCTCTGT AGGTGACGGG 
CCACCTTCTG ACATCCTGCA AGTCAAAACC CAACAGGGAG TCCCTGCCCA GCCGTCCAGC 
TTTGAGGCCG AAGCTGAGCT GGACAGTCGC ATCATGCTAA CATGGCTGTG GCCCGTCCAG 
GACCCCATCA CTAAGTATGA GCTGCAGTAC TGGGAGGCCG GCTCAGACAA CAAGTTACAT 
GTGAGCTTCA ACCCAGCGGG CTCCTACGCT GTGGACGGTC TAAAGCCCGA CACCTTGTAC 
CGTTTCTCTC TGGCAGCCCG TTCTGAGATG GGCTTAGGTG TCTACACCCA ACCCATCGAG 
GCTCGCACCG CCCAGTCCAC CCCATCAGCT CCCCCCCAGG AGGTACACCT GGTCAGCCTG 
AGCTCCACCA GCCTCAAGGT GAGTTGGGTG GCACCGCCCG CCGCTAGCCG CCACGGCGCC 
ATTGTGCGCT ACACAGTCAG CTACCAGGCC ATGGCTGGAG AAGACACGGA GCGGCACGAG 
GTGACGGGCA TCGGCGCAGA CGCCACTAGC TTTGTGCTGG AGGGCCTGGA GAAGTGGACT 
GAGTATCAGG TGTGGGTGAG GGCACACACT GACGTGGGCC CCGGCCCAGA GAGTGCCCCA 
GTGAGGATGA GAACCAAAGA GGATGTGCCG GGAGCACCCC CCAGGAAGCT GGAGGTTGAG 
GCCATAAACT CCACAGCAAT CCGGGTCACC TGGAAGCCGC CCCTGCAGGG GAAGCAGCAC 
GGCCAAATCC GGGGCTATCA AGTCATCTTC TCTCGGCTTG AAAACGGCGA ACCACGAGGC 
CAGCCTAACA TCATGGACGT TGCTTTGCCA GAGGCACAGT GGAATATTGA GGAGTCCACC 
GAGCATGAGG CCATCATTGC CGGACTGCAC TCTGAAACCA CCTACTCGGT CACCGTGGCA 
GCATACACCA CCAAAGGAGA CGGAGCGCGC AGCAAAGCCA AAGTCATCAC CACGACGGGT 
GCAGTGCCAG GAAAGCCCAC CATGATGATC AGCACCACTA TGGGCAACAC AGCGCTGATT 
CAGTGGCAGC CACCTAAGGA AATGGTTGGG GAGCTGATGG GCTACCAGCT GCAGTACAAG 
CGCACTGACG AGGAAAACTA CACCTCCCGC GAGCTGAGAA AGATCGATGA CCACTACACC 
GTCACCGGGC TGCACAAAGG TGCCACCTAT GTCTTCCGCC TCAGTGCCCG CAACCGCGCG 
GGCATCGGCG AAGCATACGT GAAAGAGATC AGCACCCCTG AAGACGTGCC GTCGGGCTTC 
CCGTTCAACC TGCAGGTCAC GGGACTGACT CAGTCCAGCA CCGAGCTGAC CTGGGACCCT 
CCGCTGGTGG CCGAAAGGAA CGGCAAGATC GTGCACTATG TGGTCGTTTA CAGGGACATC 
AACAGTCAGC AGAACAGCAC CAACCGGACG AGCGAAACGC ACATGACCAT CCAGGGCCTT 
AATCCCGACA CCACCTACGA TATCCGGGTG CAGGCGTTCA CCAGTAAGGG CGGAGGACCC 
CTCAGCCCTA GCATCCAGAG CAGGACCATG TCGAACGATT TTCCCACCCT CAACTTTGGT 
GTCAAAGCCG TCATGAAAAC ATCTGTCCTC CTCACCTGGG ATTTGCCACC GAACTACAAA 
TCCCAAGTGC CTTTCAAGAT CCTGTACAAC CAGCAAAGCG TGGAAGTGCA GGGATCCCTG 
AAGAGGAGGC TGATCACGCA GCTGCAGCCA GACACGGACT ACTCTTTTGT GCTGACAAGC 
AGGGGCAACA TTGCTGGTGG TCTACAGCAG CAGGTGTCCA TCCGCACCGC TCCCGACCTG 
ATCAACGCCA AGCCCAGCAC ACATCAGCCT CAGGGTAGCA AGATGACAAT CAACCTGCCC 
AAGGTCCAGA CCACCACGCG CGTCAGGTGG TACTACATCG TGGTGGTGCC GGTGTCCCAG 
TCAACGCAGG GGTGGAAGAA TCCAGACGAG ATGGACCTGG ACGAGCTGTT CAAGTCAAGT 
GAAGGCCCCG TCCTGAGGCG CAGACGTCAC CTGGATTCCT CCAAACCTTA CATCGCTGCC 
AAGTTGGCCT CGTTGCCGAC CACCTTCACC CTGGGTGATG AGAAGAGATA CAACGGCTTC 
TACAACAAGC CCCTGAGCAG TCCGCAGGAG TATCTCTGCT TTGTCCTGGC AGTACTCCGA 
TCCGAAGGGG CAGACAGCAC TACTAACTAT ATGACATTTT CAGCCAGTCC CTACTCAGAC 
CCGGTCCACG TGCGAAACAG CATGGCTCAG GGGATGGAGC AGCCTGAAAT GTTGTGGGTT 
ATGGGCCCTG TTTTGGCCGT GGTCCTGATT ATCATCATCG TCATCGCCAT CCTGCTCTTC 
AAGAAGAAGC GAGCGTCGCC CCTGCCCAAA GATGAGCACT CGGGCGGGGT GAAGGACTCC 
CTGCTGGCCA ACTCCTCCGA CCCTGTGGAG ATGAGAAGAC TTAACTACCA GACCCCAGGA 
ATGAGGGAGC ACCCGCCCAT CCCTGTCGTC GACCTGGCTG ACCACATAGA GCGTCTGAAG 
GCCAACGACG GCCTACGCTT TTCCCAGGAG TACGAGTCGA TTGATCCAGG GCAGCAGTTT 
ACCTGGGAGA ACTCCAACAT GGAAGTCAAC AAGCCAAAGA ATCGCTATGC AAACGTCATT 
GCCTATGACC ACTCCAGAGT GGTTCTCACC TCTGTTGACG CCGTTCCAGG CAGTGACTAC 
ATCAACGCCA ACTACATCGA CGGCTACAGG AAGCAGAACG CCTACATCGC CACGCAGGGG 
CCACTTCCAG AGACACTGAG CGACTTCTGG AGGATGGTGT GGGAGCAAAG ATCCAACACC 
ATCGTCATGA TGACCAGACT GGAGGAGAAG TCACGGGTGA AGTGTGACCA GTACTGGCCG 
TCGCGAGGAA CGGAGACCTA TGGGATGATC CAGGTCACCA TGCTGGACAC CGTGGAGTTG 
GCCACTTACA GCGTCCGTAC CTTTGCCCTC TATAAGAATG GCTCCAGTGA GAAGAGAGAG 
GTGAGACAGT TCCAGTTCAT GGCCTGGCCA GACCACGGGG TGCCAGAGTA CCCCACCCCA 
ATCCTGGCCT TCCTACGGCG GGTAAAGGCC TGCAATCCTC CAGACGCAGG GCCTATGGTG 
GTCCACTGCA GCGCGGGTGT GGGCAGGACC GGATGCTTCA TCGTGATAGA TGCCATGCTG 
GAGCGCATGA AGCACGAGAA GTCCGTAGAC ATCTACGGCC ACGTGACGTG CATGCGCGCC 
CAGAGGAACT ACATGGTCCA GACCGAGGAC CAGTACATCT TCATTCACGA AGCCCTGCTG 
GAGGCCGCCA CGTGCGGCAA CACCGAGGTG CCGGCGCGCA ACCTGTACGC CCACATCCAG 
AAACTCACCC AGCCCCCGCC CGGCGAGACG GTTACTGCCA TGGAGCTGGA ATTCAAGAGA 
CTGGCCAACT CCAAAGCCCA CACATCCCGC TTCATCAGCG CCAACTTGCC CTGCAACAAG 
TTCAAGAACC GCCTGGTCAA CATCATGCCT TTCGAGTCCA CCCGTGTCTG CCTGCAGCCC 
ATCCGTGGCG TCGAGGGCTC CGACTACATC AATGCCAGCT GCATCGACGG CTACAGACAG 
CAGAAGGCCT ACCTGGCCAC GCAGGGCCCA CTGGCAGAGA CCACCGAGGA CTTCTGGAGG 
ATGCTGTGGG AGCACAACTC CACCATCGTC GTCATGCTCA CCAAGCTACG AGAGATGGGC 
CGGGAGAAGT GCCATCAGTA CTGGCCAGCA GAGCGTTCAG CCAGGTACCA GTACTTTGTG 
GTGGATCCAA TGGCTGAGTA CAACATGCCC CAGTACATCC TCAGAGAGTT CAAAGTCACA 
GATGCCAGGG ACGGCCAGTC CAGAACAATC CGCCAATTCC AGTTCACTGA CTGGCCCGAG 
CAAGGAGTGC CAAAAACCGG AGAGGGCTTC ATTGATTTTA TCGGACAAGT GCACAAAACC 
AAGGAACAGT TCGGACAGGA CGGACCAATC ACTGTACACT GCAGTGCTGG GGTGGGCAGG 
ACCGGAGTCT TCATCACCCT CAGCATCGTG CTGGAGAGGA TGAGGTATGA AGGAGTTGTC 
GACCTCTTCC AGACAGTGAA GACACTTCGC ACCCAGCGGC CTGCCATGGT GCAGACCGAG 
GACCAGTACC AGTTGTGCTA CAGGGCAGCA TTGGAGTACC TGGGGAGCTT TGACCACTAT 
GCAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010744752.2
CDS	522..6230
Translation	MVPNIHLAVPFLPRGALLFLILLSCVVFSSLADSMPSFVKSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVEKTRTATMLCAASGNPDPEISWFKDMLPVDISSSNGRIKQLRSGALQIENSEESDQGKYECVAMNSAGTRYSAPANLYVRVRRVPPRFSIPPTNQEVMPGGSVNLTCVAVGSPMPYVKWTMGLVELTKEEEMPMGRNVLEVNNIRESANYTCVAISSLGMIEATAQVTVKALPKPPASLIVTETTATSVTLTWDSGNPEPVSYYMIQYRAKTSDNGFQEVEGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSDPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRVYYSSDLTAPLSAWQKHNTDDSSLTTISGLTPDITYSLRVLGFTSVGDGPPSDILQVKTQQGVPAQPSSFEAEAELDSRIMLTWLWPVQDPITKYELQYWEAGSDNKLHVSFNPAGSYAVDGLKPDTLYRFSLAARSEMGLGVYTQPIEARTAQSTPSAPPQEVHLVSLSSTSLKVSWVAPPAASRHGAIVRYTVSYQAMAGEDTERHEVTGIGADATSFVLEGLEKWTEYQVWVRAHTDVGPGPESAPVRMRTKEDVPGAPPRKLEVEAINSTAIRVTWKPPLQGKQHGQIRGYQVIFSRLENGEPRGQPNIMDVALPEAQWNIEESTEHEAIIAGLHSETTYSVTVAAYTTKGDGARSKAKVITTTGAVPGKPTMMISTTMGNTALIQWQPPKEMVGELMGYQLQYKRTDEENYTSRELRKIDDHYTVTGLHKGATYVFRLSARNRAGIGEAYVKEISTPEDVPSGFPFNLQVTGLTQSSTELTWDPPLVAERNGKIVHYVVVYRDINSQQNSTNRTSETHMTIQGLNPDTTYDIRVQAFTSKGGGPLSPSIQSRTMSNDFPTLNFGVKAVMKTSVLLTWDLPPNYKSQVPFKILYNQQSVEVQGSLKRRLITQLQPDTDYSFVLTSRGNIAGGLQQQVSIRTAPDLINAKPSTHQPQGSKMTINLPKVQTTTRVRWYYIVVVPVSQSTQGWKNPDEMDLDELFKSSEGPVLRRRRHLDSSKPYIAAKLASLPTTFTLGDEKRYNGFYNKPLSSPQEYLCFVLAVLRSEGADSTTNYMTFSASPYSDPVHVRNSMAQGMEQPEMLWVMGPVLAVVLIIIIVIAILLFKKKRASPLPKDEHSGGVKDSLLANSSDPVEMRRLNYQTPGMREHPPIPVVDLADHIERLKANDGLRFSQEYESIDPGQQFTWENSNMEVNKPKNRYANVIAYDHSRVVLTSVDAVPGSDYINANYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRSNTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVTMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRAQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPPPGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYLATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_010746450.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010746450.2

ORF Insert Sequence:

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTTGACATC 
AGCAGCAGCA ACGGACGCAT TAAACAGCTT CGTTCAGGAG CTCTGCAAAT TGAGAACAGC 
GAGGAGTCCG ACCAAGGGAA ATACGAGTGT GTTGCCATGA ACAGCGCTGG GACCCGTTAC 
TCCGCTCCTG CCAATCTCTA CGTACGAGTG CGAAGAGTCC CACCCAGATT CTCCATTCCG 
CCCACCAACC AAGAGGTGAT GCCAGGCGGC AGCGTCAACC TGACATGCGT GGCAGTGGGC 
TCGCCGATGC CCTACGTCAA GTGGACGATG GGCCTGGTGG AGCTGACCAA GGAGGAGGAG 
ATGCCGATGG GACGCAACGT GCTGGAGGTC AACAACATCC GCGAGTCGGC GAACTACACC 
TGCGTGGCCA TCTCCTCTCT GGGCATGATA GAAGCCACCG CTCAGGTCAC CGTCAAAGCT 
TTGCCAAAGC CTCCCGCCTC CCTCATTGTG ACTGAGACCA CAGCCACCAG TGTGACTCTC 
ACCTGGGACT CTGGAAACCC AGAACCTGTC TCCTACTACA TGATCCAATA CAGAGCCAAG 
ACCTCGGACA ACGGCTTCCA GGAAGTGGAA GGTGTGGCAA CTACCCGTTA CAGCATCGGT 
GGACTTAGCC CCTACTCTGA GTATGAGTTT CGTGTCATGG CTGTCAACAA CATCGGCCGG 
GGGCCACCGA GCGACCCCGT GGAGACACGC ACTGGTGAAC AGGCCCCCTC CTCGCCACCT 
CTGCACGTCC AAGCGCGCAT GCTGAGTGCC AGCACAATGC TGGTCCAGTG GGAACCGCCC 
GAGGAACCCA ACGGGCAAAT CAGGGGCTAC CGGGTCTACT ACAGCTCGGA CTTGACGGCT 
CCGCTCAGCG CCTGGCAGAA GCACAACACA GACGACAGCA GCCTGACGAC CATCTCGGGC 
CTGACTCCTG ATATCACCTA CAGCCTCAGG GTGCTGGGCT TCACCTCTGT AGGTGACGGG 
CCACCTTCTG ACATCCTGCA AGTCAAAACC CAACAGGGAG TCCCTGCCCA GCCGTCCAGC 
TTTGAGGCCG AAGCTGAGCT GGACAGTCGC ATCATGCTAA CATGGCTGTG GCCCGTCCAG 
GACCCCATCA CTAAGTATGA GCTGCAGTAC TGGGAGGCCG GCTCAGACAA CAAGTTACAT 
GTGAGCTTCA ACCCAGCGGG CTCCTACGCT GTGGACGGTC TAAAGCCCGA CACCTTGTAC 
CGTTTCTCTC TGGCAGCCCG TTCTGAGATG GGCTTAGGTG TCTACACCCA ACCCATCGAG 
GCTCGCACCG CCCAGTCCAC CCCATCAGCT CCCCCCCAGG AGGTACACCT GGTCAGCCTG 
AGCTCCACCA GCCTCAAGGT GAGTTGGGTG GCACCGCCCG CCGCTAGCCG CCACGGCGCC 
ATTGTGCGCT ACACAGTCAG CTACCAGGCC ATGGCTGGAG AAGACACGGA GCGGCACGAG 
GTGACGGGCA TCGGCGCAGA CGCCACTAGC TTTGTGCTGG AGGGCCTGGA GAAGTGGACT 
GAGTATCAGG TGTGGGTGAG GGCACACACT GACGTGGGCC CCGGCCCAGA GAGTGCCCCA 
GTGAGGATGA GAACCAAAGA GGATGTGCCG GGAGCACCCC CCAGGAAGCT GGAGGTTGAG 
GCCATAAACT CCACAGCAAT CCGGGTCACC TGGAAGCCGC CCCTGCAGGG GAAGCAGCAC 
GGCCAAATCC GGGGCTATCA AGTCATCTTC TCTCGGCTTG AAAACGGCGA ACCACGAGGC 
CAGCCTAACA TCATGGACGT TGCTTTGCCA GAGGCACAGT GGAATATTGA GGAGTCCACC 
GAGCATGAGG CCATCATTGC CGGACTGCAC TCTGAAACCA CCTACTCGGT CACCGTGGCA 
GCATACACCA CCAAAGGAGA CGGAGCGCGC AGCAAAGCCA AAGTCATCAC CACGACGGGT 
GCAGTGCCAG GAAAGCCCAC CATGATGATC AGCACCACTA TGGGCAACAC AGCGCTGATT 
CAGTGGCAGC CACCTAAGGA AATGGTTGGG GAGCTGATGG GCTACCAGCT GCAGTACAAG 
CGCACTGACG AGGAAAACTA CACCTCCCGC GAGCTGAGAA AGATCGATGA CCACTACACC 
GTCACCGGGC TGCACAAAGG TGCCACCTAT GTCTTCCGCC TCAGTGCCCG CAACCGCGCG 
GGCATCGGCG AAGCATACGT GAAAGAGATC AGCACCCCTG AAGACGTGCC GTCGGGCTTC 
CCGTTCAACC TGCAGGTCAC GGGACTGACT CAGTCCAGCA CCGAGCTGAC CTGGGACCCT 
CCGCTGGTGG CCGAAAGGAA CGGCAAGATC GTGCACTATG TGGTCGTTTA CAGGGACATC 
AACAGTCAGC AGAACAGCAC CAACCGGACG AGCGAAACGC ACATGACCAT CCAGGGCCTT 
AATCCCGACA CCACCTACGA TATCCGGGTG CAGGCGTTCA CCAGTAAGGG CGGAGGACCC 
CTCAGCCCTA GCATCCAGAG CAGGACCATG TCGAACGATT TTCCCACCCT CAACTTTGGT 
GTCAAAGCCG TCATGAAAAC ATCTGTCCTC CTCACCTGGG ATTTGCCACC GAACTACAAA 
TCCCAAGTGC CTTTCAAGAT CCTGTACAAC CAGCAAAGCG TGGAAGTGCA GGGATCCCTG 
AAGAGGAGGC TGATCACGCA GCTGCAGCCA GACACGGACT ACTCTTTTGT GCTGACAAGC 
AGGGGCAACA TTGCTGGTGG TCTACAGCAG CAGGTGTCCA TCCGCACCGC TCCCGACCTG 
ATCAACGCCA AGCCCAGCAC ACATCAGCCT CAGGGTAGCA AGATGACAAT CAACCTGCCC 
AAGGTCCAGA CCACCACGCG CGTCAGGTGG TACTACATCG TGGTGGTGCC GGTGTCCCAG 
TCAACGCAGG GGTGGAAGAA TCCAGACGAG ATGGACCTGG ACGAGCTGTT CAAGTCAAGT 
GAAGGCCCCG TCCTGAGGCG CAGACGTCAC CTGGATTCCT CCAAACCTTA CATCGCTGCC 
AAGTTGGCCT CGTTGCCGAC CACCTTCACC CTGGGTGATG AGAAGAGATA CAACGGCTTC 
TACAACAAGC CCCTGAGCAG TCCGCAGGAG TATCTCTGCT TTGTCCTGGC AGTACTCCGA 
TCCGAAGGGG CAGACAGCAC TACTAACTAT ATGACATTTT CAGCCAGTCC CTACTCAGAC 
CCGGTCCACG TGCGAAACAG CATGGCTCAG GGGATGGAGC AGCCTGAAAT GTTGTGGGTT 
ATGGGCCCTG TTTTGGCCGT GGTCCTGATT ATCATCATCG TCATCGCCAT CCTGCTCTTC 
AAGAAGAAGC GAGCGTCGCC CCTGCCCAAA GATGAGCACT CGGGCGGGGT GAAGGACTCC 
CTGCTGGCCA ACTCCTCCGA CCCTGTGGAG ATGAGAAGAC TTAACTACCA GACCCCAGGA 
ATGAGGGAGC ACCCGCCCAT CCCTGTCGTC GACCTGGCTG ACCACATAGA GCGTCTGAAG 
GCCAACGACG GCCTACGCTT TTCCCAGGAG TACGAGTCGA TTGATCCAGG GCAGCAGTTT 
ACCTGGGAGA ACTCCAACAT GGAAGTCAAC AAGCCAAAGA ATCGCTATGC AAACGTCATT 
GCCTATGACC ACTCCAGAGT GGTTCTCACC TCTGTTGACG CCGTTCCAGG CAGTGACTAC 
ATCAACGCCA ACTACATCGA CGGCTACAGG AAGCAGAACG CCTACATCGC CACGCAGGGG 
CCACTTCCAG AGACACTGAG CGACTTCTGG AGGATGGTGT GGGAGCAAAG ATCCAACACC 
ATCGTCATGA TGACCAGACT GGAGGAGAAG TCACGGGTGA AGTGTGACCA GTACTGGCCG 
TCGCGAGGAA CGGAGACCTA TGGGATGATC CAGGTCACCA TGCTGGACAC CGTGGAGTTG 
GCCACTTACA GCGTCCGTAC CTTTGCCCTC TATAAGAATG GCTCCAGTGA GAAGAGAGAG 
GTGAGACAGT TCCAGTTCAT GGCCTGGCCA GACCACGGGG TGCCAGAGTA CCCCACCCCA 
ATCCTGGCCT TCCTACGGCG GGTAAAGGCC TGCAATCCTC CAGACGCAGG GCCTATGGTG 
GTCCACTGCA GCGCGGGTGT GGGCAGGACC GGATGCTTCA TCGTGATAGA TGCCATGCTG 
GAGCGCATGA AGCACGAGAA GTCCGTAGAC ATCTACGGCC ACGTGACGTG CATGCGCGCC 
CAGAGGAACT ACATGGTCCA GACCGAGGAC CAGTACATCT TCATTCACGA AGCCCTGCTG 
GAGGCCGCCA CGTGCGGCAA CACCGAGGTG CCGGCGCGCA ACCTGTACGC CCACATCCAG 
AAACTCACCC AGCCCCCGCC CGGCGAGACG GTTACTGCCA TGGAGCTGGA ATTCAAGAGA 
CTGGCCAACT CCAAAGCCCA CACATCCCGC TTCATCAGCG CCAACTTGCC CTGCAACAAG 
TTCAAGAACC GCCTGGTCAA CATCATGCCT TTCGAGTCCA CCCGTGTCTG CCTGCAGCCC 
ATCCGTGGCG TCGAGGGCTC CGACTACATC AATGCCAGCT GCATCGACGG CTACAGACAG 
CAGAAGGCCT ACCTGGCCAC GCAGGGCCCA CTGGCAGAGA CCACCGAGGA CTTCTGGAGG 
ATGCTGTGGG AGCACAACTC CACCATCGTC GTCATGCTCA CCAAGCTACG AGAGATGGGC 
CGGGAGAAGT GCCATCAGTA CTGGCCAGCA GAGCGTTCAG CCAGGTACCA GTACTTTGTG 
GTGGATCCAA TGGCTGAGTA CAACATGCCC CAGTACATCC TCAGAGAGTT CAAAGTCACA 
GATGCCAGGG ACGGCCAGTC CAGAACAATC CGCCAATTCC AGTTCACTGA CTGGCCCGAG 
CAAGGAGTGC CAAAAACCGG AGAGGGCTTC ATTGATTTTA TCGGACAAGT GCACAAAACC 
AAGGAACAGT TCGGACAGGA CGGACCAATC ACTGTACACT GCAGTGCTGG GGTGGGCAGG 
ACCGGAGTCT TCATCACCCT CAGCATCGTG CTGGAGAGGA TGAGGTATGA AGGAGTTGTC 
GACCTCTTCC AGACAGTGAA GACACTTCGC ACCCAGCGGC CTGCCATGGT GCAGACCGAG 
GACCAGTACC AGTTGTGCTA CAGGGCAGCA TTGGAGTACC TGGGGAGCTT TGACCACTAT 
GCAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104931439 ( XM_010746453.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010744755.2 Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X13, mRNA.

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CloneID	OLa84607
Clone ID Related Accession (Same CDS sequence)	XM_010746453.2
Accession Version	XM_010746453.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	receptor-type tyrosine-protein phosphatase F isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017607936.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 29, 2016 this sequence version replaced XM_010746453.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTTGACATC 
AGCAGCAGCA ACGGACGCAT TAAACAGCTT CGTTCAGGAG CTCTGCAAAT TGAGAACAGC 
GAGGAGTCCG ACCAAGGGAA ATACGAGTGT GTTGCCATGA ACAGCGCTGG GACCCGTTAC 
TCCGCTCCTG CCAATCTCTA CGTACGAGTG CGAAGAGTCC CACCCAGATT CTCCATTCCG 
CCCACCAACC AAGAGGTGAT GCCAGGCGGC AGCGTCAACC TGACATGCGT GGCAGTGGGC 
TCGCCGATGC CCTACGTCAA GTGGACGATG GGCCTGGTGG AGCTGACCAA GGAGGAGGAG 
ATGCCGATGG GACGCAACGT GCTGGAGGTC AACAACATCC GCGAGTCGGC GAACTACACC 
TGCGTGGCCA TCTCCTCTCT GGGCATGATA GAAGCCACCG CTCAGGTCAC CGTCAAAGCT 
TTGCCAAAGC CTCCCGCCTC CCTCATTGTG ACTGAGACCA CAGCCACCAG TGTGACTCTC 
ACCTGGGACT CTGGAAACCC AGAACCTGTC TCCTACTACA TGATCCAATA CAGAGCCAAG 
ACCTCGGACA ACGGCTTCCA GGAAGTGGAA GGTGTGGCAA CTACCCGTTA CAGCATCGGT 
GGACTTAGCC CCTACTCTGA GTATGAGTTT CGTGTCATGG CTGTCAACAA CATCGGCCGG 
GGGCCACCGA GCGACCCCGT GGAGACACGC ACTGGTGAAC AGGCCCCCTC CTCGCCACCT 
CTGCACGTCC AAGCGCGCAT GCTGAGTGCC AGCACAATGC TGGTCCAGTG GGAACCGCCC 
GAGGAACCCA ACGGGCAAAT CAGGGGCTAC CGGGTCTACT ACAGCTCGGA CTTGACGGCT 
CCGCTCAGCG CCTGGCAGAA GCACAACACA GACGACAGCA GCCTGACGAC CATCTCGGGC 
CTGACTCCTG ATATCACCTA CAGCCTCAGG GTGCTGGGCT TCACCTCTGT AGGTGACGGG 
CCACCTTCTG ACATCCTGCA AGTCAAAACC CAACAGGGAG TCCCTGCCCA GCCGTCCAGC 
TTTGAGGCCG AAGCTGAGCT GGACAGTCGC ATCATGCTAA CATGGCTGTG GCCCGTCCAG 
GACCCCATCA CTAAGTATGA GCTGCAGTAC TGGGAGGCCG GCTCAGACAA CAAGTTACAT 
GTGAGCTTCA ACCCAGCGGG CTCCTACGCT GTGGACGGTC TAAAGCCCGA CACCTTGTAC 
CGTTTCTCTC TGGCAGCCCG TTCTGAGATG GGCTTAGGTG TCTACACCCA ACCCATCGAG 
GCTCGCACCG CCCAGTCCAC CCCATCAGCT CCCCCCCAGG AGGTACACCT GGTCAGCCTG 
AGCTCCACCA GCCTCAAGGT GAGTTGGGTG GCACCGCCCG CCGCTAGCCG CCACGGCGCC 
ATTGTGCGCT ACACAGTCAG CTACCAGGCC ATGGCTGGAG AAGACACGGA GCGGCACGAG 
GTGACGGGCA TCGGCGCAGA CGCCACTAGC TTTGTGCTGG AGGGCCTGGA GAAGTGGACT 
GAGTATCAGG TGTGGGTGAG GGCACACACT GACGTGGGCC CCGGCCCAGA GAGTGCCCCA 
GTGAGGATGA GAACCAAAGA GGATGTGCCG GGAGCACCCC CCAGGAAGCT GGAGGTTGAG 
GCCATAAACT CCACAGCAAT CCGGGTCACC TGGAAGCCGC CCCTGCAGGG GAAGCAGCAC 
GGCCAAATCC GGGGCTATCA AGTCATCTTC TCTCGGCTTG AAAACGGCGA ACCACGAGGC 
CAGCCTAACA TCATGGACGT TGCTTTGCCA GAGGCACAGG AGGCCATCAT TGCCGGACTG 
CACTCTGAAA CCACCTACTC GGTCACCGTG GCAGCATACA CCACCAAAGG AGACGGAGCG 
CGCAGCAAAG CCAAAGTCAT CACCACGACG GGTGCAGTGC CAGGAAAGCC CACCATGATG 
ATCAGCACCA CTATGGGCAA CACAGCGCTG ATTCAGTGGC AGCCACCTAA GGAAATGGTT 
GGGGAGCTGA TGGGCTACCA GCTGCAGTAC AAGCGCACTG ACGAGGAAAA CTACACCTCC 
CGCGAGCTGA GAAAGATCGA TGACCACTAC ACCGTCACCG GGCTGCACAA AGGTGCCACC 
TATGTCTTCC GCCTCAGTGC CCGCAACCGC GCGGGCATCG GCGAAGCATA CGTGAAAGAG 
ATCAGCACCC CTGAAGACGT GCCGTCGGGC TTCCCGTTCA ACCTGCAGGT CACGGGACTG 
ACTCAGTCCA GCACCGAGCT GACCTGGGAC CCTCCGCTGG TGGCCGAAAG GAACGGCAAG 
ATCGTGCACT ATGTGGTCGT TTACAGGGAC ATCAACAGTC AGCAGAACAG CACCAACCGG 
ACGAGCGAAA CGCACATGAC CATCCAGGGC CTTAATCCCG ACACCACCTA CGATATCCGG 
GTGCAGGCGT TCACCAGTAA GGGCGGAGGA CCCCTCAGCC CTAGCATCCA GAGCAGGACC 
ATGTCGAACG ATTTTCCCAC CCTCAACTTT GGTGTCAAAG CCGTCATGAA AACATCTGTC 
CTCCTCACCT GGGATTTGCC ACCGAACTAC AAATCCCAAG TGCCTTTCAA GATCCTGTAC 
AACCAGCAAA GCGTGGAAGT GCAGGGATCC CTGAAGAGGA GGCTGATCAC GCAGCTGCAG 
CCAGACACGG ACTACTCTTT TGTGCTGACA AGCAGGGGCA ACATTGCTGG TGGTCTACAG 
CAGCAGGTGT CCATCCGCAC CGCTCCCGAC CTGATCAACG CCAAGCCCAG CACACATCAG 
CCTCAGGGTA GCAAGATGAC AATCAACCTG CCCAAGGTCC AGACCACCAC GCGCGTCAGG 
TGGTACTACA TCGTGGTGGT GCCGGTGTCC CAGTCAACGC AGGGGTGGAA GAATCCAGAC 
GAGATGGACC TGGACGAGCT GTTCAAGTCA AGTGAAGGCC CCGTCCTGAG GCGCAGACGT 
CACCTGGATT CCTCCAAACC TTACATCGCT GCCAAGTTGG CCTCGTTGCC GACCACCTTC 
ACCCTGGGTG ATGAGAAGAG ATACAACGGC TTCTACAACA AGCCCCTGAG CAGTCCGCAG 
GAGTATCTCT GCTTTGTCCT GGCAGTACTC CGATCCGAAG GGGCAGACAG CACTACTAAC 
TATATGACAT TTTCAGCCAG TCCCTACTCA GACCCGGTCC ACGTGCGAAA CAGCATGGCT 
CAGGGGATGG AGCAGCCTGA AATGTTGTGG GTTATGGGCC CTGTTTTGGC CGTGGTCCTG 
ATTATCATCA TCGTCATCGC CATCCTGCTC TTCAAGAAGA AGCGAGCGTC GCCCCTGCCC 
AAAGATGAGC ACTCGGGCGG GGTGAAGGAC TCCCTGCTGG CCAACTCCTC CGACCCTGTG 
GAGATGAGAA GACTTAACTA CCAGACCCCA GGAATGAGGG AGCACCCGCC CATCCCTGTC 
GTCGACCTGG CTGACCACAT AGAGCGTCTG AAGGCCAACG ACGGCCTACG CTTTTCCCAG 
GAGTACGAGT CGATTGATCC AGGGCAGCAG TTTACCTGGG AGAACTCCAA CATGGAAGTC 
AACAAGCCAA AGAATCGCTA TGCAAACGTC ATTGCCTATG ACCACTCCAG AGTGGTTCTC 
ACCTCTGTTG ACGCCGTTCC AGGCAGTGAC TACATCAACG CCAACTACAT CGACGGCTAC 
AGGAAGCAGA ACGCCTACAT CGCCACGCAG GGGCCACTTC CAGAGACACT GAGCGACTTC 
TGGAGGATGG TGTGGGAGCA AAGATCCAAC ACCATCGTCA TGATGACCAG ACTGGAGGAG 
AAGTCACGGG TGAAGTGTGA CCAGTACTGG CCGTCGCGAG GAACGGAGAC CTATGGGATG 
ATCCAGGTCA CCATGCTGGA CACCGTGGAG TTGGCCACTT ACAGCGTCCG TACCTTTGCC 
CTCTATAAGA ATGGCTCCAG TGAGAAGAGA GAGGTGAGAC AGTTCCAGTT CATGGCCTGG 
CCAGACCACG GGGTGCCAGA GTACCCCACC CCAATCCTGG CCTTCCTACG GCGGGTAAAG 
GCCTGCAATC CTCCAGACGC AGGGCCTATG GTGGTCCACT GCAGCGCGGG TGTGGGCAGG 
ACCGGATGCT TCATCGTGAT AGATGCCATG CTGGAGCGCA TGAAGCACGA GAAGTCCGTA 
GACATCTACG GCCACGTGAC GTGCATGCGC GCCCAGAGGA ACTACATGGT CCAGACCGAG 
GACCAGTACA TCTTCATTCA CGAAGCCCTG CTGGAGGCCG CCACGTGCGG CAACACCGAG 
GTGCCGGCGC GCAACCTGTA CGCCCACATC CAGAAACTCA CCCAGCCCCC GCCCGGCGAG 
ACGGTTACTG CCATGGAGCT GGAATTCAAG AGACTGGCCA ACTCCAAAGC CCACACATCC 
CGCTTCATCA GCGCCAACTT GCCCTGCAAC AAGTTCAAGA ACCGCCTGGT CAACATCATG 
CCTTTCGAGT CCACCCGTGT CTGCCTGCAG CCCATCCGTG GCGTCGAGGG CTCCGACTAC 
ATCAATGCCA GCTGCATCGA CGGCTACAGA CAGCAGAAGG CCTACCTGGC CACGCAGGGC 
CCACTGGCAG AGACCACCGA GGACTTCTGG AGGATGCTGT GGGAGCACAA CTCCACCATC 
GTCGTCATGC TCACCAAGCT ACGAGAGATG GGCCGGGAGA AGTGCCATCA GTACTGGCCA 
GCAGAGCGTT CAGCCAGGTA CCAGTACTTT GTGGTGGATC CAATGGCTGA GTACAACATG 
CCCCAGTACA TCCTCAGAGA GTTCAAAGTC ACAGATGCCA GGGACGGCCA GTCCAGAACA 
ATCCGCCAAT TCCAGTTCAC TGACTGGCCC GAGCAAGGAG TGCCAAAAAC CGGAGAGGGC 
TTCATTGATT TTATCGGACA AGTGCACAAA ACCAAGGAAC AGTTCGGACA GGACGGACCA 
ATCACTGTAC ACTGCAGTGC TGGGGTGGGC AGGACCGGAG TCTTCATCAC CCTCAGCATC 
GTGCTGGAGA GGATGAGGTA TGAAGGAGTT GTCGACCTCT TCCAGACAGT GAAGACACTT 
CGCACCCAGC GGCCTGCCAT GGTGCAGACC GAGGACCAGT ACCAGTTGTG CTACAGGGCA 
GCATTGGAGT ACCTGGGGAG CTTTGACCAC TATGCAACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010744755.2
CDS	522..6203
Translation	MVPNIHLAVPFLPRGALLFLILLSCVVFSSLADSMPSFVKSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVEKTRTATMLCAASGNPDPEISWFKDMLPVDISSSNGRIKQLRSGALQIENSEESDQGKYECVAMNSAGTRYSAPANLYVRVRRVPPRFSIPPTNQEVMPGGSVNLTCVAVGSPMPYVKWTMGLVELTKEEEMPMGRNVLEVNNIRESANYTCVAISSLGMIEATAQVTVKALPKPPASLIVTETTATSVTLTWDSGNPEPVSYYMIQYRAKTSDNGFQEVEGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSDPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRVYYSSDLTAPLSAWQKHNTDDSSLTTISGLTPDITYSLRVLGFTSVGDGPPSDILQVKTQQGVPAQPSSFEAEAELDSRIMLTWLWPVQDPITKYELQYWEAGSDNKLHVSFNPAGSYAVDGLKPDTLYRFSLAARSEMGLGVYTQPIEARTAQSTPSAPPQEVHLVSLSSTSLKVSWVAPPAASRHGAIVRYTVSYQAMAGEDTERHEVTGIGADATSFVLEGLEKWTEYQVWVRAHTDVGPGPESAPVRMRTKEDVPGAPPRKLEVEAINSTAIRVTWKPPLQGKQHGQIRGYQVIFSRLENGEPRGQPNIMDVALPEAQEAIIAGLHSETTYSVTVAAYTTKGDGARSKAKVITTTGAVPGKPTMMISTTMGNTALIQWQPPKEMVGELMGYQLQYKRTDEENYTSRELRKIDDHYTVTGLHKGATYVFRLSARNRAGIGEAYVKEISTPEDVPSGFPFNLQVTGLTQSSTELTWDPPLVAERNGKIVHYVVVYRDINSQQNSTNRTSETHMTIQGLNPDTTYDIRVQAFTSKGGGPLSPSIQSRTMSNDFPTLNFGVKAVMKTSVLLTWDLPPNYKSQVPFKILYNQQSVEVQGSLKRRLITQLQPDTDYSFVLTSRGNIAGGLQQQVSIRTAPDLINAKPSTHQPQGSKMTINLPKVQTTTRVRWYYIVVVPVSQSTQGWKNPDEMDLDELFKSSEGPVLRRRRHLDSSKPYIAAKLASLPTTFTLGDEKRYNGFYNKPLSSPQEYLCFVLAVLRSEGADSTTNYMTFSASPYSDPVHVRNSMAQGMEQPEMLWVMGPVLAVVLIIIIVIAILLFKKKRASPLPKDEHSGGVKDSLLANSSDPVEMRRLNYQTPGMREHPPIPVVDLADHIERLKANDGLRFSQEYESIDPGQQFTWENSNMEVNKPKNRYANVIAYDHSRVVLTSVDAVPGSDYINANYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRSNTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVTMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRAQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPPPGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYLATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_010746453.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010746453.2

ORF Insert Sequence:

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTTGACATC 
AGCAGCAGCA ACGGACGCAT TAAACAGCTT CGTTCAGGAG CTCTGCAAAT TGAGAACAGC 
GAGGAGTCCG ACCAAGGGAA ATACGAGTGT GTTGCCATGA ACAGCGCTGG GACCCGTTAC 
TCCGCTCCTG CCAATCTCTA CGTACGAGTG CGAAGAGTCC CACCCAGATT CTCCATTCCG 
CCCACCAACC AAGAGGTGAT GCCAGGCGGC AGCGTCAACC TGACATGCGT GGCAGTGGGC 
TCGCCGATGC CCTACGTCAA GTGGACGATG GGCCTGGTGG AGCTGACCAA GGAGGAGGAG 
ATGCCGATGG GACGCAACGT GCTGGAGGTC AACAACATCC GCGAGTCGGC GAACTACACC 
TGCGTGGCCA TCTCCTCTCT GGGCATGATA GAAGCCACCG CTCAGGTCAC CGTCAAAGCT 
TTGCCAAAGC CTCCCGCCTC CCTCATTGTG ACTGAGACCA CAGCCACCAG TGTGACTCTC 
ACCTGGGACT CTGGAAACCC AGAACCTGTC TCCTACTACA TGATCCAATA CAGAGCCAAG 
ACCTCGGACA ACGGCTTCCA GGAAGTGGAA GGTGTGGCAA CTACCCGTTA CAGCATCGGT 
GGACTTAGCC CCTACTCTGA GTATGAGTTT CGTGTCATGG CTGTCAACAA CATCGGCCGG 
GGGCCACCGA GCGACCCCGT GGAGACACGC ACTGGTGAAC AGGCCCCCTC CTCGCCACCT 
CTGCACGTCC AAGCGCGCAT GCTGAGTGCC AGCACAATGC TGGTCCAGTG GGAACCGCCC 
GAGGAACCCA ACGGGCAAAT CAGGGGCTAC CGGGTCTACT ACAGCTCGGA CTTGACGGCT 
CCGCTCAGCG CCTGGCAGAA GCACAACACA GACGACAGCA GCCTGACGAC CATCTCGGGC 
CTGACTCCTG ATATCACCTA CAGCCTCAGG GTGCTGGGCT TCACCTCTGT AGGTGACGGG 
CCACCTTCTG ACATCCTGCA AGTCAAAACC CAACAGGGAG TCCCTGCCCA GCCGTCCAGC 
TTTGAGGCCG AAGCTGAGCT GGACAGTCGC ATCATGCTAA CATGGCTGTG GCCCGTCCAG 
GACCCCATCA CTAAGTATGA GCTGCAGTAC TGGGAGGCCG GCTCAGACAA CAAGTTACAT 
GTGAGCTTCA ACCCAGCGGG CTCCTACGCT GTGGACGGTC TAAAGCCCGA CACCTTGTAC 
CGTTTCTCTC TGGCAGCCCG TTCTGAGATG GGCTTAGGTG TCTACACCCA ACCCATCGAG 
GCTCGCACCG CCCAGTCCAC CCCATCAGCT CCCCCCCAGG AGGTACACCT GGTCAGCCTG 
AGCTCCACCA GCCTCAAGGT GAGTTGGGTG GCACCGCCCG CCGCTAGCCG CCACGGCGCC 
ATTGTGCGCT ACACAGTCAG CTACCAGGCC ATGGCTGGAG AAGACACGGA GCGGCACGAG 
GTGACGGGCA TCGGCGCAGA CGCCACTAGC TTTGTGCTGG AGGGCCTGGA GAAGTGGACT 
GAGTATCAGG TGTGGGTGAG GGCACACACT GACGTGGGCC CCGGCCCAGA GAGTGCCCCA 
GTGAGGATGA GAACCAAAGA GGATGTGCCG GGAGCACCCC CCAGGAAGCT GGAGGTTGAG 
GCCATAAACT CCACAGCAAT CCGGGTCACC TGGAAGCCGC CCCTGCAGGG GAAGCAGCAC 
GGCCAAATCC GGGGCTATCA AGTCATCTTC TCTCGGCTTG AAAACGGCGA ACCACGAGGC 
CAGCCTAACA TCATGGACGT TGCTTTGCCA GAGGCACAGG AGGCCATCAT TGCCGGACTG 
CACTCTGAAA CCACCTACTC GGTCACCGTG GCAGCATACA CCACCAAAGG AGACGGAGCG 
CGCAGCAAAG CCAAAGTCAT CACCACGACG GGTGCAGTGC CAGGAAAGCC CACCATGATG 
ATCAGCACCA CTATGGGCAA CACAGCGCTG ATTCAGTGGC AGCCACCTAA GGAAATGGTT 
GGGGAGCTGA TGGGCTACCA GCTGCAGTAC AAGCGCACTG ACGAGGAAAA CTACACCTCC 
CGCGAGCTGA GAAAGATCGA TGACCACTAC ACCGTCACCG GGCTGCACAA AGGTGCCACC 
TATGTCTTCC GCCTCAGTGC CCGCAACCGC GCGGGCATCG GCGAAGCATA CGTGAAAGAG 
ATCAGCACCC CTGAAGACGT GCCGTCGGGC TTCCCGTTCA ACCTGCAGGT CACGGGACTG 
ACTCAGTCCA GCACCGAGCT GACCTGGGAC CCTCCGCTGG TGGCCGAAAG GAACGGCAAG 
ATCGTGCACT ATGTGGTCGT TTACAGGGAC ATCAACAGTC AGCAGAACAG CACCAACCGG 
ACGAGCGAAA CGCACATGAC CATCCAGGGC CTTAATCCCG ACACCACCTA CGATATCCGG 
GTGCAGGCGT TCACCAGTAA GGGCGGAGGA CCCCTCAGCC CTAGCATCCA GAGCAGGACC 
ATGTCGAACG ATTTTCCCAC CCTCAACTTT GGTGTCAAAG CCGTCATGAA AACATCTGTC 
CTCCTCACCT GGGATTTGCC ACCGAACTAC AAATCCCAAG TGCCTTTCAA GATCCTGTAC 
AACCAGCAAA GCGTGGAAGT GCAGGGATCC CTGAAGAGGA GGCTGATCAC GCAGCTGCAG 
CCAGACACGG ACTACTCTTT TGTGCTGACA AGCAGGGGCA ACATTGCTGG TGGTCTACAG 
CAGCAGGTGT CCATCCGCAC CGCTCCCGAC CTGATCAACG CCAAGCCCAG CACACATCAG 
CCTCAGGGTA GCAAGATGAC AATCAACCTG CCCAAGGTCC AGACCACCAC GCGCGTCAGG 
TGGTACTACA TCGTGGTGGT GCCGGTGTCC CAGTCAACGC AGGGGTGGAA GAATCCAGAC 
GAGATGGACC TGGACGAGCT GTTCAAGTCA AGTGAAGGCC CCGTCCTGAG GCGCAGACGT 
CACCTGGATT CCTCCAAACC TTACATCGCT GCCAAGTTGG CCTCGTTGCC GACCACCTTC 
ACCCTGGGTG ATGAGAAGAG ATACAACGGC TTCTACAACA AGCCCCTGAG CAGTCCGCAG 
GAGTATCTCT GCTTTGTCCT GGCAGTACTC CGATCCGAAG GGGCAGACAG CACTACTAAC 
TATATGACAT TTTCAGCCAG TCCCTACTCA GACCCGGTCC ACGTGCGAAA CAGCATGGCT 
CAGGGGATGG AGCAGCCTGA AATGTTGTGG GTTATGGGCC CTGTTTTGGC CGTGGTCCTG 
ATTATCATCA TCGTCATCGC CATCCTGCTC TTCAAGAAGA AGCGAGCGTC GCCCCTGCCC 
AAAGATGAGC ACTCGGGCGG GGTGAAGGAC TCCCTGCTGG CCAACTCCTC CGACCCTGTG 
GAGATGAGAA GACTTAACTA CCAGACCCCA GGAATGAGGG AGCACCCGCC CATCCCTGTC 
GTCGACCTGG CTGACCACAT AGAGCGTCTG AAGGCCAACG ACGGCCTACG CTTTTCCCAG 
GAGTACGAGT CGATTGATCC AGGGCAGCAG TTTACCTGGG AGAACTCCAA CATGGAAGTC 
AACAAGCCAA AGAATCGCTA TGCAAACGTC ATTGCCTATG ACCACTCCAG AGTGGTTCTC 
ACCTCTGTTG ACGCCGTTCC AGGCAGTGAC TACATCAACG CCAACTACAT CGACGGCTAC 
AGGAAGCAGA ACGCCTACAT CGCCACGCAG GGGCCACTTC CAGAGACACT GAGCGACTTC 
TGGAGGATGG TGTGGGAGCA AAGATCCAAC ACCATCGTCA TGATGACCAG ACTGGAGGAG 
AAGTCACGGG TGAAGTGTGA CCAGTACTGG CCGTCGCGAG GAACGGAGAC CTATGGGATG 
ATCCAGGTCA CCATGCTGGA CACCGTGGAG TTGGCCACTT ACAGCGTCCG TACCTTTGCC 
CTCTATAAGA ATGGCTCCAG TGAGAAGAGA GAGGTGAGAC AGTTCCAGTT CATGGCCTGG 
CCAGACCACG GGGTGCCAGA GTACCCCACC CCAATCCTGG CCTTCCTACG GCGGGTAAAG 
GCCTGCAATC CTCCAGACGC AGGGCCTATG GTGGTCCACT GCAGCGCGGG TGTGGGCAGG 
ACCGGATGCT TCATCGTGAT AGATGCCATG CTGGAGCGCA TGAAGCACGA GAAGTCCGTA 
GACATCTACG GCCACGTGAC GTGCATGCGC GCCCAGAGGA ACTACATGGT CCAGACCGAG 
GACCAGTACA TCTTCATTCA CGAAGCCCTG CTGGAGGCCG CCACGTGCGG CAACACCGAG 
GTGCCGGCGC GCAACCTGTA CGCCCACATC CAGAAACTCA CCCAGCCCCC GCCCGGCGAG 
ACGGTTACTG CCATGGAGCT GGAATTCAAG AGACTGGCCA ACTCCAAAGC CCACACATCC 
CGCTTCATCA GCGCCAACTT GCCCTGCAAC AAGTTCAAGA ACCGCCTGGT CAACATCATG 
CCTTTCGAGT CCACCCGTGT CTGCCTGCAG CCCATCCGTG GCGTCGAGGG CTCCGACTAC 
ATCAATGCCA GCTGCATCGA CGGCTACAGA CAGCAGAAGG CCTACCTGGC CACGCAGGGC 
CCACTGGCAG AGACCACCGA GGACTTCTGG AGGATGCTGT GGGAGCACAA CTCCACCATC 
GTCGTCATGC TCACCAAGCT ACGAGAGATG GGCCGGGAGA AGTGCCATCA GTACTGGCCA 
GCAGAGCGTT CAGCCAGGTA CCAGTACTTT GTGGTGGATC CAATGGCTGA GTACAACATG 
CCCCAGTACA TCCTCAGAGA GTTCAAAGTC ACAGATGCCA GGGACGGCCA GTCCAGAACA 
ATCCGCCAAT TCCAGTTCAC TGACTGGCCC GAGCAAGGAG TGCCAAAAAC CGGAGAGGGC 
TTCATTGATT TTATCGGACA AGTGCACAAA ACCAAGGAAC AGTTCGGACA GGACGGACCA 
ATCACTGTAC ACTGCAGTGC TGGGGTGGGC AGGACCGGAG TCTTCATCAC CCTCAGCATC 
GTGCTGGAGA GGATGAGGTA TGAAGGAGTT GTCGACCTCT TCCAGACAGT GAAGACACTT 
CGCACCCAGC GGCCTGCCAT GGTGCAGACC GAGGACCAGT ACCAGTTGTG CTACAGGGCA 
GCATTGGAGT ACCTGGGGAG CTTTGACCAC TATGCAACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104931439 ( XM_010746454.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010744756.2 Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X14, mRNA.

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CloneID	OLa84608
Clone ID Related Accession (Same CDS sequence)	XM_010746454.2
Accession Version	XM_010746454.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	receptor-type tyrosine-protein phosphatase F isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017607936.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 29, 2016 this sequence version replaced XM_010746454.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTTGACATC 
AGCAGCAGCA ACGGACGCAT TAAACAGCTT CGTTCAGGAG CTCTGCAAAT TGAGAACAGC 
GAGGAGTCCG ACCAAGGGAA ATACGAGTGT GTTGCCATGA ACAGCGCTGG GACCCGTTAC 
TCCGCTCCTG CCAATCTCTA CGTACGAGTG CGAAGAGTCC CACCCAGATT CTCCATTCCG 
CCCACCAACC AAGAGGTGAT GCCAGGCGGC AGCGTCAACC TGACATGCGT GGCAGTGGGC 
TCGCCGATGC CCTACGTCAA GTGGACGATG GGCCTGGTGG AGCTGACCAA GGAGGAGGAG 
ATGCCGATGG GACGCAACGT GCTGGAGGTC AACAACATCC GCGAGTCGGC GAACTACACC 
TGCGTGGCCA TCTCCTCTCT GGGCATGATA GAAGCCACCG CTCAGGTCAC CGTCAAAGCT 
TTGCCAAAGC CTCCCGCCTC CCTCATTGTG ACTGAGACCA CAGCCACCAG TGTGACTCTC 
ACCTGGGACT CTGGAAACCC AGAACCTGTC TCCTACTACA TGATCCAATA CAGAGCCAAG 
ACCTCGGACA ACGGCTTCCA GGAAGTGGAA GGTGTGGCAA CTACCCGTTA CAGCATCGGT 
GGACTTAGCC CCTACTCTGA GTATGAGTTT CGTGTCATGG CTGTCAACAA CATCGGCCGG 
GGGCCACCGA GCGACCCCGT GGAGACACGC ACTGGTGAAC AGGCCCCCTC CTCGCCACCT 
CTGCACGTCC AAGCGCGCAT GCTGAGTGCC AGCACAATGC TGGTCCAGTG GGAACCGCCC 
GAGGAACCCA ACGGGCAAAT CAGGGGCTAC CGGGTCTACT ACAGCTCGGA CTTGACGGCT 
CCGCTCAGCG CCTGGCAGAA GCACAACACA GACGACAGCA GCCTGACGAC CATCTCGGGC 
CTGACTCCTG ATATCACCTA CAGCCTCAGG GTGCTGGGCT TCACCTCTGT AGGTGACGGG 
CCACCTTCTG ACATCCTGCA AGTCAAAACC CAACAGGGAG TCCCTGCCCA GCCGTCCAGC 
TTTGAGGCCG AAGCTGAGCT GGACAGTCGC ATCATGCTAA CATGGCTGTG GCCCGTCCAG 
GACCCCATCA CTAAGTATGA GCTGCAGTAC TGGGAGGCCG GCTCAGACAA CAAGTTACAT 
GTGAGCTTCA ACCCAGCGGG CTCCTACGCT GTGGACGGTC TAAAGCCCGA CACCTTGTAC 
CGTTTCTCTC TGGCAGCCCG TTCTGAGATG GGCTTAGGTG TCTACACCCA ACCCATCGAG 
GCTCGCACCG CCCAGTCCAT GCCGGGAGCA CCCCCCAGGA AGCTGGAGGT TGAGGCCATA 
AACTCCACAG CAATCCGGGT CACCTGGAAG CCGCCCCTGC AGGGGAAGCA GCACGGCCAA 
ATCCGGGGCT ATCAAGTCAT CTTCTCTCGG CTTGAAAACG GCGAACCACG AGGCCAGCCT 
AACATCATGG ACGTTGCTTT GCCAGAGGCA CAGGAGGCCA TCATTGCCGG ACTGCACTCT 
GAAACCACCT ACTCGGTCAC CGTGGCAGCA TACACCACCA AAGGAGACGG AGCGCGCAGC 
AAAGCCAAAG TCATCACCAC GACGGGTGCA GTGCCAGGAA AGCCCACCAT GATGATCAGC 
ACCACTATGG GCAACACAGC GCTGATTCAG TGGCAGCCAC CTAAGGAAAT GGTTGGGGAG 
CTGATGGGCT ACCAGCTGCA GTACAAGCGC ACTGACGAGG AAAACTACAC CTCCCGCGAG 
CTGAGAAAGA TCGATGACCA CTACACCGTC ACCGGGCTGC ACAAAGGTGC CACCTATGTC 
TTCCGCCTCA GTGCCCGCAA CCGCGCGGGC ATCGGCGAAG CATACGTGAA AGAGATCAGC 
ACCCCTGAAG ACGTGCCGTC GGGCTTCCCG TTCAACCTGC AGGTCACGGG ACTGACTCAG 
TCCAGCACCG AGCTGACCTG GGACCCTCCG CTGGTGGCCG AAAGGAACGG CAAGATCGTG 
CACTATGTGG TCGTTTACAG GGACATCAAC AGTCAGCAGA ACAGCACCAA CCGGACGAGC 
GAAACGCACA TGACCATCCA GGGCCTTAAT CCCGACACCA CCTACGATAT CCGGGTGCAG 
GCGTTCACCA GTAAGGGCGG AGGACCCCTC AGCCCTAGCA TCCAGAGCAG GACCATGTCG 
AACGATTTTC CCACCCTCAA CTTTGGTGTC AAAGCCGTCA TGAAAACATC TGTCCTCCTC 
ACCTGGGATT TGCCACCGAA CTACAAATCC CAAGTGCCTT TCAAGATCCT GTACAACCAG 
CAAAGCGTGG AAGTGCAGGG ATCCCTGAAG AGGAGGCTGA TCACGCAGCT GCAGCCAGAC 
ACGGACTACT CTTTTGTGCT GACAAGCAGG GGCAACATTG CTGGTGGTCT ACAGCAGCAG 
GTGTCCATCC GCACCGCTCC CGACCTGATC AACGCCAAGC CCAGCACACA TCAGCCTCAG 
GGTAGCAAGA TGACAATCAA CCTGCCCAAG GTCCAGACCA CCACGCGCGT CAGGTGGTAC 
TACATCGTGG TGGTGCCGGT GTCCCAGTCA ACGCAGGGGT GGAAGAATCC AGACGAGATG 
GACCTGGACG AGCTGTTCAA GTCAAGTGAA GGCCCCGTCC TGAGGCGCAG ACGTCACCTG 
GATTCCTCCA AACCTTACAT CGCTGCCAAG TTGGCCTCGT TGCCGACCAC CTTCACCCTG 
GGTGATGAGA AGAGATACAA CGGCTTCTAC AACAAGCCCC TGAGCAGTCC GCAGGAGTAT 
CTCTGCTTTG TCCTGGCAGT ACTCCGATCC GAAGGGGCAG ACAGCACTAC TAACTATATG 
ACATTTTCAG CCAGTCCCTA CTCAGACCCG GTCCACGTGC GAAACAGCAT GGCTCAGGGG 
ATGGAGCAGC CTGAAATGTT GTGGGTTATG GGCCCTGTTT TGGCCGTGGT CCTGATTATC 
ATCATCGTCA TCGCCATCCT GCTCTTCAAG AAGAAGCGAG CGTCGCCCCT GCCCAAAGAT 
GAGCACTCGG GCGGGGTGAA GGACTCCCTG CTGGCCAACT CCTCCGACCC TGTGGAGATG 
AGAAGACTTA ACTACCAGAC CCCAGGAATG AGGGAGCACC CGCCCATCCC TGTCGTCGAC 
CTGGCTGACC ACATAGAGCG TCTGAAGGCC AACGACGGCC TACGCTTTTC CCAGGAGTAC 
GAGTCGATTG ATCCAGGGCA GCAGTTTACC TGGGAGAACT CCAACATGGA AGTCAACAAG 
CCAAAGAATC GCTATGCAAA CGTCATTGCC TATGACCACT CCAGAGTGGT TCTCACCTCT 
GTTGACGCCG TTCCAGGCAG TGACTACATC AACGCCAACT ACATCGACGG CTACAGGAAG 
CAGAACGCCT ACATCGCCAC GCAGGGGCCA CTTCCAGAGA CACTGAGCGA CTTCTGGAGG 
ATGGTGTGGG AGCAAAGATC CAACACCATC GTCATGATGA CCAGACTGGA GGAGAAGTCA 
CGGGTGAAGT GTGACCAGTA CTGGCCGTCG CGAGGAACGG AGACCTATGG GATGATCCAG 
GTCACCATGC TGGACACCGT GGAGTTGGCC ACTTACAGCG TCCGTACCTT TGCCCTCTAT 
AAGAATGGCT CCAGTGAGAA GAGAGAGGTG AGACAGTTCC AGTTCATGGC CTGGCCAGAC 
CACGGGGTGC CAGAGTACCC CACCCCAATC CTGGCCTTCC TACGGCGGGT AAAGGCCTGC 
AATCCTCCAG ACGCAGGGCC TATGGTGGTC CACTGCAGCG CGGGTGTGGG CAGGACCGGA 
TGCTTCATCG TGATAGATGC CATGCTGGAG CGCATGAAGC ACGAGAAGTC CGTAGACATC 
TACGGCCACG TGACGTGCAT GCGCGCCCAG AGGAACTACA TGGTCCAGAC CGAGGACCAG 
TACATCTTCA TTCACGAAGC CCTGCTGGAG GCCGCCACGT GCGGCAACAC CGAGGTGCCG 
GCGCGCAACC TGTACGCCCA CATCCAGAAA CTCACCCAGC CCCCGCCCGG CGAGACGGTT 
ACTGCCATGG AGCTGGAATT CAAGAGACTG GCCAACTCCA AAGCCCACAC ATCCCGCTTC 
ATCAGCGCCA ACTTGCCCTG CAACAAGTTC AAGAACCGCC TGGTCAACAT CATGCCTTTC 
GAGTCCACCC GTGTCTGCCT GCAGCCCATC CGTGGCGTCG AGGGCTCCGA CTACATCAAT 
GCCAGCTGCA TCGACGGCTA CAGACAGCAG AAGGCCTACC TGGCCACGCA GGGCCCACTG 
GCAGAGACCA CCGAGGACTT CTGGAGGATG CTGTGGGAGC ACAACTCCAC CATCGTCGTC 
ATGCTCACCA AGCTACGAGA GATGGGCCGG GAGAAGTGCC ATCAGTACTG GCCAGCAGAG 
CGTTCAGCCA GGTACCAGTA CTTTGTGGTG GATCCAATGG CTGAGTACAA CATGCCCCAG 
TACATCCTCA GAGAGTTCAA AGTCACAGAT GCCAGGGACG GCCAGTCCAG AACAATCCGC 
CAATTCCAGT TCACTGACTG GCCCGAGCAA GGAGTGCCAA AAACCGGAGA GGGCTTCATT 
GATTTTATCG GACAAGTGCA CAAAACCAAG GAACAGTTCG GACAGGACGG ACCAATCACT 
GTACACTGCA GTGCTGGGGT GGGCAGGACC GGAGTCTTCA TCACCCTCAG CATCGTGCTG 
GAGAGGATGA GGTATGAAGG AGTTGTCGAC CTCTTCCAGA CAGTGAAGAC ACTTCGCACC 
CAGCGGCCTG CCATGGTGCA GACCGAGGAC CAGTACCAGT TGTGCTACAG GGCAGCATTG 
GAGTACCTGG GGAGCTTTGA CCACTATGCA ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010744756.2
CDS	520..5895
Translation	MVPNIHLAVPFLPRGALLFLILLSCVVFSSLADSMPSFVKSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVEKTRTATMLCAASGNPDPEISWFKDMLPVDISSSNGRIKQLRSGALQIENSEESDQGKYECVAMNSAGTRYSAPANLYVRVRRVPPRFSIPPTNQEVMPGGSVNLTCVAVGSPMPYVKWTMGLVELTKEEEMPMGRNVLEVNNIRESANYTCVAISSLGMIEATAQVTVKALPKPPASLIVTETTATSVTLTWDSGNPEPVSYYMIQYRAKTSDNGFQEVEGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSDPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRVYYSSDLTAPLSAWQKHNTDDSSLTTISGLTPDITYSLRVLGFTSVGDGPPSDILQVKTQQGVPAQPSSFEAEAELDSRIMLTWLWPVQDPITKYELQYWEAGSDNKLHVSFNPAGSYAVDGLKPDTLYRFSLAARSEMGLGVYTQPIEARTAQSMPGAPPRKLEVEAINSTAIRVTWKPPLQGKQHGQIRGYQVIFSRLENGEPRGQPNIMDVALPEAQEAIIAGLHSETTYSVTVAAYTTKGDGARSKAKVITTTGAVPGKPTMMISTTMGNTALIQWQPPKEMVGELMGYQLQYKRTDEENYTSRELRKIDDHYTVTGLHKGATYVFRLSARNRAGIGEAYVKEISTPEDVPSGFPFNLQVTGLTQSSTELTWDPPLVAERNGKIVHYVVVYRDINSQQNSTNRTSETHMTIQGLNPDTTYDIRVQAFTSKGGGPLSPSIQSRTMSNDFPTLNFGVKAVMKTSVLLTWDLPPNYKSQVPFKILYNQQSVEVQGSLKRRLITQLQPDTDYSFVLTSRGNIAGGLQQQVSIRTAPDLINAKPSTHQPQGSKMTINLPKVQTTTRVRWYYIVVVPVSQSTQGWKNPDEMDLDELFKSSEGPVLRRRRHLDSSKPYIAAKLASLPTTFTLGDEKRYNGFYNKPLSSPQEYLCFVLAVLRSEGADSTTNYMTFSASPYSDPVHVRNSMAQGMEQPEMLWVMGPVLAVVLIIIIVIAILLFKKKRASPLPKDEHSGGVKDSLLANSSDPVEMRRLNYQTPGMREHPPIPVVDLADHIERLKANDGLRFSQEYESIDPGQQFTWENSNMEVNKPKNRYANVIAYDHSRVVLTSVDAVPGSDYINANYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRSNTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVTMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRAQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPPPGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYLATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_010746454.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010746454.2

ORF Insert Sequence:

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTTGACATC 
AGCAGCAGCA ACGGACGCAT TAAACAGCTT CGTTCAGGAG CTCTGCAAAT TGAGAACAGC 
GAGGAGTCCG ACCAAGGGAA ATACGAGTGT GTTGCCATGA ACAGCGCTGG GACCCGTTAC 
TCCGCTCCTG CCAATCTCTA CGTACGAGTG CGAAGAGTCC CACCCAGATT CTCCATTCCG 
CCCACCAACC AAGAGGTGAT GCCAGGCGGC AGCGTCAACC TGACATGCGT GGCAGTGGGC 
TCGCCGATGC CCTACGTCAA GTGGACGATG GGCCTGGTGG AGCTGACCAA GGAGGAGGAG 
ATGCCGATGG GACGCAACGT GCTGGAGGTC AACAACATCC GCGAGTCGGC GAACTACACC 
TGCGTGGCCA TCTCCTCTCT GGGCATGATA GAAGCCACCG CTCAGGTCAC CGTCAAAGCT 
TTGCCAAAGC CTCCCGCCTC CCTCATTGTG ACTGAGACCA CAGCCACCAG TGTGACTCTC 
ACCTGGGACT CTGGAAACCC AGAACCTGTC TCCTACTACA TGATCCAATA CAGAGCCAAG 
ACCTCGGACA ACGGCTTCCA GGAAGTGGAA GGTGTGGCAA CTACCCGTTA CAGCATCGGT 
GGACTTAGCC CCTACTCTGA GTATGAGTTT CGTGTCATGG CTGTCAACAA CATCGGCCGG 
GGGCCACCGA GCGACCCCGT GGAGACACGC ACTGGTGAAC AGGCCCCCTC CTCGCCACCT 
CTGCACGTCC AAGCGCGCAT GCTGAGTGCC AGCACAATGC TGGTCCAGTG GGAACCGCCC 
GAGGAACCCA ACGGGCAAAT CAGGGGCTAC CGGGTCTACT ACAGCTCGGA CTTGACGGCT 
CCGCTCAGCG CCTGGCAGAA GCACAACACA GACGACAGCA GCCTGACGAC CATCTCGGGC 
CTGACTCCTG ATATCACCTA CAGCCTCAGG GTGCTGGGCT TCACCTCTGT AGGTGACGGG 
CCACCTTCTG ACATCCTGCA AGTCAAAACC CAACAGGGAG TCCCTGCCCA GCCGTCCAGC 
TTTGAGGCCG AAGCTGAGCT GGACAGTCGC ATCATGCTAA CATGGCTGTG GCCCGTCCAG 
GACCCCATCA CTAAGTATGA GCTGCAGTAC TGGGAGGCCG GCTCAGACAA CAAGTTACAT 
GTGAGCTTCA ACCCAGCGGG CTCCTACGCT GTGGACGGTC TAAAGCCCGA CACCTTGTAC 
CGTTTCTCTC TGGCAGCCCG TTCTGAGATG GGCTTAGGTG TCTACACCCA ACCCATCGAG 
GCTCGCACCG CCCAGTCCAT GCCGGGAGCA CCCCCCAGGA AGCTGGAGGT TGAGGCCATA 
AACTCCACAG CAATCCGGGT CACCTGGAAG CCGCCCCTGC AGGGGAAGCA GCACGGCCAA 
ATCCGGGGCT ATCAAGTCAT CTTCTCTCGG CTTGAAAACG GCGAACCACG AGGCCAGCCT 
AACATCATGG ACGTTGCTTT GCCAGAGGCA CAGGAGGCCA TCATTGCCGG ACTGCACTCT 
GAAACCACCT ACTCGGTCAC CGTGGCAGCA TACACCACCA AAGGAGACGG AGCGCGCAGC 
AAAGCCAAAG TCATCACCAC GACGGGTGCA GTGCCAGGAA AGCCCACCAT GATGATCAGC 
ACCACTATGG GCAACACAGC GCTGATTCAG TGGCAGCCAC CTAAGGAAAT GGTTGGGGAG 
CTGATGGGCT ACCAGCTGCA GTACAAGCGC ACTGACGAGG AAAACTACAC CTCCCGCGAG 
CTGAGAAAGA TCGATGACCA CTACACCGTC ACCGGGCTGC ACAAAGGTGC CACCTATGTC 
TTCCGCCTCA GTGCCCGCAA CCGCGCGGGC ATCGGCGAAG CATACGTGAA AGAGATCAGC 
ACCCCTGAAG ACGTGCCGTC GGGCTTCCCG TTCAACCTGC AGGTCACGGG ACTGACTCAG 
TCCAGCACCG AGCTGACCTG GGACCCTCCG CTGGTGGCCG AAAGGAACGG CAAGATCGTG 
CACTATGTGG TCGTTTACAG GGACATCAAC AGTCAGCAGA ACAGCACCAA CCGGACGAGC 
GAAACGCACA TGACCATCCA GGGCCTTAAT CCCGACACCA CCTACGATAT CCGGGTGCAG 
GCGTTCACCA GTAAGGGCGG AGGACCCCTC AGCCCTAGCA TCCAGAGCAG GACCATGTCG 
AACGATTTTC CCACCCTCAA CTTTGGTGTC AAAGCCGTCA TGAAAACATC TGTCCTCCTC 
ACCTGGGATT TGCCACCGAA CTACAAATCC CAAGTGCCTT TCAAGATCCT GTACAACCAG 
CAAAGCGTGG AAGTGCAGGG ATCCCTGAAG AGGAGGCTGA TCACGCAGCT GCAGCCAGAC 
ACGGACTACT CTTTTGTGCT GACAAGCAGG GGCAACATTG CTGGTGGTCT ACAGCAGCAG 
GTGTCCATCC GCACCGCTCC CGACCTGATC AACGCCAAGC CCAGCACACA TCAGCCTCAG 
GGTAGCAAGA TGACAATCAA CCTGCCCAAG GTCCAGACCA CCACGCGCGT CAGGTGGTAC 
TACATCGTGG TGGTGCCGGT GTCCCAGTCA ACGCAGGGGT GGAAGAATCC AGACGAGATG 
GACCTGGACG AGCTGTTCAA GTCAAGTGAA GGCCCCGTCC TGAGGCGCAG ACGTCACCTG 
GATTCCTCCA AACCTTACAT CGCTGCCAAG TTGGCCTCGT TGCCGACCAC CTTCACCCTG 
GGTGATGAGA AGAGATACAA CGGCTTCTAC AACAAGCCCC TGAGCAGTCC GCAGGAGTAT 
CTCTGCTTTG TCCTGGCAGT ACTCCGATCC GAAGGGGCAG ACAGCACTAC TAACTATATG 
ACATTTTCAG CCAGTCCCTA CTCAGACCCG GTCCACGTGC GAAACAGCAT GGCTCAGGGG 
ATGGAGCAGC CTGAAATGTT GTGGGTTATG GGCCCTGTTT TGGCCGTGGT CCTGATTATC 
ATCATCGTCA TCGCCATCCT GCTCTTCAAG AAGAAGCGAG CGTCGCCCCT GCCCAAAGAT 
GAGCACTCGG GCGGGGTGAA GGACTCCCTG CTGGCCAACT CCTCCGACCC TGTGGAGATG 
AGAAGACTTA ACTACCAGAC CCCAGGAATG AGGGAGCACC CGCCCATCCC TGTCGTCGAC 
CTGGCTGACC ACATAGAGCG TCTGAAGGCC AACGACGGCC TACGCTTTTC CCAGGAGTAC 
GAGTCGATTG ATCCAGGGCA GCAGTTTACC TGGGAGAACT CCAACATGGA AGTCAACAAG 
CCAAAGAATC GCTATGCAAA CGTCATTGCC TATGACCACT CCAGAGTGGT TCTCACCTCT 
GTTGACGCCG TTCCAGGCAG TGACTACATC AACGCCAACT ACATCGACGG CTACAGGAAG 
CAGAACGCCT ACATCGCCAC GCAGGGGCCA CTTCCAGAGA CACTGAGCGA CTTCTGGAGG 
ATGGTGTGGG AGCAAAGATC CAACACCATC GTCATGATGA CCAGACTGGA GGAGAAGTCA 
CGGGTGAAGT GTGACCAGTA CTGGCCGTCG CGAGGAACGG AGACCTATGG GATGATCCAG 
GTCACCATGC TGGACACCGT GGAGTTGGCC ACTTACAGCG TCCGTACCTT TGCCCTCTAT 
AAGAATGGCT CCAGTGAGAA GAGAGAGGTG AGACAGTTCC AGTTCATGGC CTGGCCAGAC 
CACGGGGTGC CAGAGTACCC CACCCCAATC CTGGCCTTCC TACGGCGGGT AAAGGCCTGC 
AATCCTCCAG ACGCAGGGCC TATGGTGGTC CACTGCAGCG CGGGTGTGGG CAGGACCGGA 
TGCTTCATCG TGATAGATGC CATGCTGGAG CGCATGAAGC ACGAGAAGTC CGTAGACATC 
TACGGCCACG TGACGTGCAT GCGCGCCCAG AGGAACTACA TGGTCCAGAC CGAGGACCAG 
TACATCTTCA TTCACGAAGC CCTGCTGGAG GCCGCCACGT GCGGCAACAC CGAGGTGCCG 
GCGCGCAACC TGTACGCCCA CATCCAGAAA CTCACCCAGC CCCCGCCCGG CGAGACGGTT 
ACTGCCATGG AGCTGGAATT CAAGAGACTG GCCAACTCCA AAGCCCACAC ATCCCGCTTC 
ATCAGCGCCA ACTTGCCCTG CAACAAGTTC AAGAACCGCC TGGTCAACAT CATGCCTTTC 
GAGTCCACCC GTGTCTGCCT GCAGCCCATC CGTGGCGTCG AGGGCTCCGA CTACATCAAT 
GCCAGCTGCA TCGACGGCTA CAGACAGCAG AAGGCCTACC TGGCCACGCA GGGCCCACTG 
GCAGAGACCA CCGAGGACTT CTGGAGGATG CTGTGGGAGC ACAACTCCAC CATCGTCGTC 
ATGCTCACCA AGCTACGAGA GATGGGCCGG GAGAAGTGCC ATCAGTACTG GCCAGCAGAG 
CGTTCAGCCA GGTACCAGTA CTTTGTGGTG GATCCAATGG CTGAGTACAA CATGCCCCAG 
TACATCCTCA GAGAGTTCAA AGTCACAGAT GCCAGGGACG GCCAGTCCAG AACAATCCGC 
CAATTCCAGT TCACTGACTG GCCCGAGCAA GGAGTGCCAA AAACCGGAGA GGGCTTCATT 
GATTTTATCG GACAAGTGCA CAAAACCAAG GAACAGTTCG GACAGGACGG ACCAATCACT 
GTACACTGCA GTGCTGGGGT GGGCAGGACC GGAGTCTTCA TCACCCTCAG CATCGTGCTG 
GAGAGGATGA GGTATGAAGG AGTTGTCGAC CTCTTCCAGA CAGTGAAGAC ACTTCGCACC 
CAGCGGCCTG CCATGGTGCA GACCGAGGAC CAGTACCAGT TGTGCTACAG GGCAGCATTG 
GAGTACCTGG GGAGCTTTGA CCACTATGCA ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104931439 ( XM_019263588.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019119133.1 Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X15, mRNA.

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CloneID	OLa84609
Clone ID Related Accession (Same CDS sequence)	XM_019263588.1
Accession Version	XM_019263588.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	receptor-type tyrosine-protein phosphatase F isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017607936.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTTGACATC 
AGCAGCAGCA ACGGACGCAT TAAACAGCTT CGTTCAGGTG GTACACCAAT TAGAGGAGCT 
CTGCAAATTG AGAACAGCGA GGAGTCCGAC CAAGGGAAAT ACGAGTGTGT TGCCATGAAC 
AGCGCTGGGA CCCGTTACTC CGCTCCTGCC AATCTCTACG TACGAGTGCG AAGAGTCCCA 
CCCAGATTCT CCATTCCGCC CACCAACCAA GAGGTGATGC CAGGCGGCAG CGTCAACCTG 
ACATGCGTGG CAGTGGGCTC GCCGATGCCC TACGTCAAGT GGACGATGGG CCTGGTGGAG 
CTGACCAAGG AGGAGGAGAT GCCGATGGGA CGCAACGTGC TGGAGGTCAA CAACATCCGC 
GAGTCGGCGA ACTACACCTG CGTGGCCATC TCCTCTCTGG GCATGATAGA AGCCACCGCT 
CAGGTCACCG TCAAAGCTTT GCCAAAGCCT CCCGCCTCCC TCATTGTGAC TGAGACCACA 
GCCACCAGTG TGACTCTCAC CTGGGACTCT GGAAACCCAG AACCTGTCTC CTACTACATG 
ATCCAATACA GAGCCAAGAC CTCGGACAAC GGCTTCCAGG AAGTGGAAGG TGTGGCAACT 
ACCCGTTACA GCATCGGTGG ACTTAGCCCC TACTCTGAGT ATGAGTTTCG TGTCATGGCT 
GTCAACAACA TCGGCCGGGG GCCACCGAGC GACCCCGTGG AGACACGCAC TGGTGAACAG 
GCCCCCTCCT CGCCACCTCT GCACGTCCAA GCGCGCATGC TGAGTGCCAG CACAATGCTG 
GTCCAGTGGG AACCGCCCGA GGAACCCAAC GGGCAAATCA GGGGCTACCG GGTCTACTAC 
AGCTCGGACT TGACGGCTCC GCTCAGCGCC TGGCAGAAGC ACAACACAGA CGACAGCAGC 
CTGACGACCA TCTCGGGCCT GACTCCTGAT ATCACCTACA GCCTCAGGGT GCTGGGCTTC 
ACCTCTGTAG GTGACGGGCC ACCTTCTGAC ATCCTGCAAG TCAAAACCCA ACAGGGAGTC 
CCTGCCCAGC CGTCCAGCTT TGAGGCCGAA GCTGAGCTGG ACAGTCGCAT CATGCTAACA 
TGGCTGTGGC CCGTCCAGGA CCCCATCACT AAGTATGAGC TGCAGTACTG GGAGGCCGGC 
TCAGACAACA AGTTACATGT GAGCTTCAAC CCAGCGGGCT CCTACGCTGT GGACGGTCTA 
AAGCCCGACA CCTTGTACCG TTTCTCTCTG GCAGCCCGTT CTGAGATGGG CTTAGGTGTC 
TACACCCAAC CCATCGAGGC TCGCACCGCC CAGTCCATGC CGGGAGCACC CCCCAGGAAG 
CTGGAGGTTG AGGCCATAAA CTCCACAGCA ATCCGGGTCA CCTGGAAGCC GCCCCTGCAG 
GGGAAGCAGC ACGGCCAAAT CCGGGGCTAT CAAGTCATCT TCTCTCGGCT TGAAAACGGC 
GAACCACGAG GCCAGCCTAA CATCATGGAC GTTGCTTTGC CAGAGGCACA GTGGAATATT 
GAGGAGTCCA CCGAGCATGA GGCCATCATT GCCGGACTGC ACTCTGAAAC CACCTACTCG 
GTCACCGTGG CAGCATACAC CACCAAAGGA GACGGAGCGC GCAGCAAAGC CAAAGTCATC 
ACCACGACGG GTGCAGTGCC AGGAAAGCCC ACCATGATGA TCAGCACCAC TATGGGCAAC 
ACAGCGCTGA TTCAGTGGCA GCCACCTAAG GAAATGGTTG GGGAGCTGAT GGGCTACCAG 
CTGCAGTACA AGCGCACTGA CGAGGAAAAC TACACCTCCC GCGAGCTGAG AAAGATCGAT 
GACCACTACA CCGTCACCGG GCTGCACAAA GGTGCCACCT ATGTCTTCCG CCTCAGTGCC 
CGCAACCGCG CGGGCATCGG CGAAGCATAC GTGAAAGAGA TCAGCACCCC TGAAGACGTG 
CCGTCGGGCT TCCCGTTCAA CCTGCAGGTC ACGGGACTGA CTCAGTCCAG CACCGAGCTG 
ACCTGGGACC CTCCGCTGGT GGCCGAAAGG AACGGCAAGA TCGTGCACTA TGTGGTCGTT 
TACAGGGACA TCAACAGTCA GCAGAACAGC ACCAACCGGA CGAGCGAAAC GCACATGACC 
ATCCAGGGCC TTAATCCCGA CACCACCTAC GATATCCGGG TGCAGGCGTT CACCAGTAAG 
GGCGGAGGAC CCCTCAGCCC TAGCATCCAG AGCAGGACCA TGTCGAACGA TTTTCCCACC 
CTCAACTTTG GTGTCAAAGC CGTCATGAAA ACATCTGTCC TCCTCACCTG GGATTTGCCA 
CCGAACTACA AATCCCAAGT GCCTTTCAAG ATCCTGTACA ACCAGCAAAG CGTGGAAGTG 
CAGGGATCCC TGAAGAGGAG GCTGATCACG CAGCTGCAGC CAGACACGGA CTACTCTTTT 
GTGCTGACAA GCAGGGGCAA CATTGCTGGT GGTCTACAGC AGCAGGTGTC CATCCGCACC 
GCTCCCGACC TGATCAACGC CAAGCCCAGC ACACATCAGC CTCAGGGTAG CAAGATGACA 
ATCAACCTGC CCAAGGTCCA GACCACCACG CGCGTCAGGT GGTACTACAT CGTGGTGGTG 
CCGGTGTCCC AGTCAACGCA GGGGTGGAAG AATCCAGACG AGATGGACCT GGACGAGCTG 
TTCAAGTCAA GTGAAGGCCC CGTCCTGAGG CGCAGACGTC ACCTGGATTC CTCCAAACCT 
TACATCGCTG CCAAGTTGGC CTCGTTGCCG ACCACCTTCA CCCTGGGTGA TGAGAAGAGA 
TACAACGGCT TCTACAACAA GCCCCTGAGC AGTCCGCAGG AGTATCTCTG CTTTGTCCTG 
GCAGTACTCC GATCCGAAGG GGCAGACAGC ACTACTAACT ATATGACATT TTCAGCCAGT 
CCCTACTCAG ACCCGGTCCA CGTGCGAAAC AGCATGGCTC AGGGGATGGA GCAGCCTGAA 
ATGTTGTGGG TTATGGGCCC TGTTTTGGCC GTGGTCCTGA TTATCATCAT CGTCATCGCC 
ATCCTGCTCT TCAAGAAGAA GCGAGCGTCG CCCCTGCCCA AAGATGAGCA CTCGGGCGGG 
GTGAAGGACT CCCTGCTGGC CAACTCCTCC GACCCTGTGG AGATGAGAAG ACTTAACTAC 
CAGACCCCAG GTCCCAGCTC TCATCGCTGC CCCAACACTC CAAGAATGAG GGAGCACCCG 
CCCATCCCTG TCGTCGACCT GGCTGACCAC ATAGAGCGTC TGAAGGCCAA CGACGGCCTA 
CGCTTTTCCC AGGAGTACGA GTCGATTGAT CCAGGGCAGC AGTTTACCTG GGAGAACTCC 
AACATGGAAG TCAACAAGCC AAAGAATCGC TATGCAAACG TCATTGCCTA TGACCACTCC 
AGAGTGGTTC TCACCTCTGT TGACGCCGTT CCAGGCAGTG ACTACATCAA CGCCAACTAC 
ATCGACGGCT ACAGGAAGCA GAACGCCTAC ATCGCCACGC AGGGGCCACT TCCAGAGACA 
CTGAGCGACT TCTGGAGGAT GGTGTGGGAG CAAAGATCCA ACACCATCGT CATGATGACC 
AGACTGGAGG AGAAGTCACG GGTGAAGTGT GACCAGTACT GGCCGTCGCG AGGAACGGAG 
ACCTATGGGA TGATCCAGGT CACCATGCTG GACACCGTGG AGTTGGCCAC TTACAGCGTC 
CGTACCTTTG CCCTCTATAA GAATGGCTCC AGTGAGAAGA GAGAGGTGAG ACAGTTCCAG 
TTCATGGCCT GGCCAGACCA CGGGGTGCCA GAGTACCCCA CCCCAATCCT GGCCTTCCTA 
CGGCGGGTAA AGGCCTGCAA TCCTCCAGAC GCAGGGCCTA TGGTGGTCCA CTGCAGCGCG 
GGTGTGGGCA GGACCGGATG CTTCATCGTG ATAGATGCCA TGCTGGAGCG CATGAAGCAC 
GAGAAGTCCG TAGACATCTA CGGCCACGTG ACGTGCATGC GCGCCCAGAG GAACTACATG 
GTCCAGACCG AGGACCAGTA CATCTTCATT CACGAAGCCC TGCTGGAGGC CGCCACGTGC 
GGCAACACCG AGGTGCCGGC GCGCAACCTG TACGCCCACA TCCAGAAACT CACCCAGCCC 
CCGCCCGGCG AGACGGTTAC TGCCATGGAG CTGGAATTCA AGAGACTGGC CAACTCCAAA 
GCCCACACAT CCCGCTTCAT CAGCGCCAAC TTGCCCTGCA ACAAGTTCAA GAACCGCCTG 
GTCAACATCA TGCCTTTCGA GTCCACCCGT GTCTGCCTGC AGCCCATCCG TGGCGTCGAG 
GGCTCCGACT ACATCAATGC CAGCTGCATC GACGGCTACA GACAGCAGAA GGCCTACCTG 
GCCACGCAGG GCCCACTGGC AGAGACCACC GAGGACTTCT GGAGGATGCT GTGGGAGCAC 
AACTCCACCA TCGTCGTCAT GCTCACCAAG CTACGAGAGA TGGGCCGGGA GAAGTGCCAT 
CAGTACTGGC CAGCAGAGCG TTCAGCCAGG TACCAGTACT TTGTGGTGGA TCCAATGGCT 
GAGTACAACA TGCCCCAGTA CATCCTCAGA GAGTTCAAAG TCACAGATGC CAGGGACGGC 
CAGTCCAGAA CAATCCGCCA ATTCCAGTTC ACTGACTGGC CCGAGCAAGG AGTGCCAAAA 
ACCGGAGAGG GCTTCATTGA TTTTATCGGA CAAGTGCACA AAACCAAGGA ACAGTTCGGA 
CAGGACGGAC CAATCACTGT ACACTGCAGT GCTGGGGTGG GCAGGACCGG AGTCTTCATC 
ACCCTCAGCA TCGTGCTGGA GAGGATGAGG TATGAAGGAG TTGTCGACCT CTTCCAGACA 
GTGAAGACAC TTCGCACCCA GCGGCCTGCC ATGGTGCAGA CCGAGGACCA GTACCAGTTG 
TGCTACAGGG CAGCATTGGA GTACCTGGGG AGCTTTGACC ACTATGCAAC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019119133.1
CDS	521..5974
Translation	MVPNIHLAVPFLPRGALLFLILLSCVVFSSLADSMPSFVKSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVEKTRTATMLCAASGNPDPEISWFKDMLPVDISSSNGRIKQLRSGGTPIRGALQIENSEESDQGKYECVAMNSAGTRYSAPANLYVRVRRVPPRFSIPPTNQEVMPGGSVNLTCVAVGSPMPYVKWTMGLVELTKEEEMPMGRNVLEVNNIRESANYTCVAISSLGMIEATAQVTVKALPKPPASLIVTETTATSVTLTWDSGNPEPVSYYMIQYRAKTSDNGFQEVEGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSDPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRVYYSSDLTAPLSAWQKHNTDDSSLTTISGLTPDITYSLRVLGFTSVGDGPPSDILQVKTQQGVPAQPSSFEAEAELDSRIMLTWLWPVQDPITKYELQYWEAGSDNKLHVSFNPAGSYAVDGLKPDTLYRFSLAARSEMGLGVYTQPIEARTAQSMPGAPPRKLEVEAINSTAIRVTWKPPLQGKQHGQIRGYQVIFSRLENGEPRGQPNIMDVALPEAQWNIEESTEHEAIIAGLHSETTYSVTVAAYTTKGDGARSKAKVITTTGAVPGKPTMMISTTMGNTALIQWQPPKEMVGELMGYQLQYKRTDEENYTSRELRKIDDHYTVTGLHKGATYVFRLSARNRAGIGEAYVKEISTPEDVPSGFPFNLQVTGLTQSSTELTWDPPLVAERNGKIVHYVVVYRDINSQQNSTNRTSETHMTIQGLNPDTTYDIRVQAFTSKGGGPLSPSIQSRTMSNDFPTLNFGVKAVMKTSVLLTWDLPPNYKSQVPFKILYNQQSVEVQGSLKRRLITQLQPDTDYSFVLTSRGNIAGGLQQQVSIRTAPDLINAKPSTHQPQGSKMTINLPKVQTTTRVRWYYIVVVPVSQSTQGWKNPDEMDLDELFKSSEGPVLRRRRHLDSSKPYIAAKLASLPTTFTLGDEKRYNGFYNKPLSSPQEYLCFVLAVLRSEGADSTTNYMTFSASPYSDPVHVRNSMAQGMEQPEMLWVMGPVLAVVLIIIIVIAILLFKKKRASPLPKDEHSGGVKDSLLANSSDPVEMRRLNYQTPGPSSHRCPNTPRMREHPPIPVVDLADHIERLKANDGLRFSQEYESIDPGQQFTWENSNMEVNKPKNRYANVIAYDHSRVVLTSVDAVPGSDYINANYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRSNTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVTMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRAQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPPPGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYLATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_019263588.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019263588.1

ORF Insert Sequence:

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTTGACATC 
AGCAGCAGCA ACGGACGCAT TAAACAGCTT CGTTCAGGTG GTACACCAAT TAGAGGAGCT 
CTGCAAATTG AGAACAGCGA GGAGTCCGAC CAAGGGAAAT ACGAGTGTGT TGCCATGAAC 
AGCGCTGGGA CCCGTTACTC CGCTCCTGCC AATCTCTACG TACGAGTGCG AAGAGTCCCA 
CCCAGATTCT CCATTCCGCC CACCAACCAA GAGGTGATGC CAGGCGGCAG CGTCAACCTG 
ACATGCGTGG CAGTGGGCTC GCCGATGCCC TACGTCAAGT GGACGATGGG CCTGGTGGAG 
CTGACCAAGG AGGAGGAGAT GCCGATGGGA CGCAACGTGC TGGAGGTCAA CAACATCCGC 
GAGTCGGCGA ACTACACCTG CGTGGCCATC TCCTCTCTGG GCATGATAGA AGCCACCGCT 
CAGGTCACCG TCAAAGCTTT GCCAAAGCCT CCCGCCTCCC TCATTGTGAC TGAGACCACA 
GCCACCAGTG TGACTCTCAC CTGGGACTCT GGAAACCCAG AACCTGTCTC CTACTACATG 
ATCCAATACA GAGCCAAGAC CTCGGACAAC GGCTTCCAGG AAGTGGAAGG TGTGGCAACT 
ACCCGTTACA GCATCGGTGG ACTTAGCCCC TACTCTGAGT ATGAGTTTCG TGTCATGGCT 
GTCAACAACA TCGGCCGGGG GCCACCGAGC GACCCCGTGG AGACACGCAC TGGTGAACAG 
GCCCCCTCCT CGCCACCTCT GCACGTCCAA GCGCGCATGC TGAGTGCCAG CACAATGCTG 
GTCCAGTGGG AACCGCCCGA GGAACCCAAC GGGCAAATCA GGGGCTACCG GGTCTACTAC 
AGCTCGGACT TGACGGCTCC GCTCAGCGCC TGGCAGAAGC ACAACACAGA CGACAGCAGC 
CTGACGACCA TCTCGGGCCT GACTCCTGAT ATCACCTACA GCCTCAGGGT GCTGGGCTTC 
ACCTCTGTAG GTGACGGGCC ACCTTCTGAC ATCCTGCAAG TCAAAACCCA ACAGGGAGTC 
CCTGCCCAGC CGTCCAGCTT TGAGGCCGAA GCTGAGCTGG ACAGTCGCAT CATGCTAACA 
TGGCTGTGGC CCGTCCAGGA CCCCATCACT AAGTATGAGC TGCAGTACTG GGAGGCCGGC 
TCAGACAACA AGTTACATGT GAGCTTCAAC CCAGCGGGCT CCTACGCTGT GGACGGTCTA 
AAGCCCGACA CCTTGTACCG TTTCTCTCTG GCAGCCCGTT CTGAGATGGG CTTAGGTGTC 
TACACCCAAC CCATCGAGGC TCGCACCGCC CAGTCCATGC CGGGAGCACC CCCCAGGAAG 
CTGGAGGTTG AGGCCATAAA CTCCACAGCA ATCCGGGTCA CCTGGAAGCC GCCCCTGCAG 
GGGAAGCAGC ACGGCCAAAT CCGGGGCTAT CAAGTCATCT TCTCTCGGCT TGAAAACGGC 
GAACCACGAG GCCAGCCTAA CATCATGGAC GTTGCTTTGC CAGAGGCACA GTGGAATATT 
GAGGAGTCCA CCGAGCATGA GGCCATCATT GCCGGACTGC ACTCTGAAAC CACCTACTCG 
GTCACCGTGG CAGCATACAC CACCAAAGGA GACGGAGCGC GCAGCAAAGC CAAAGTCATC 
ACCACGACGG GTGCAGTGCC AGGAAAGCCC ACCATGATGA TCAGCACCAC TATGGGCAAC 
ACAGCGCTGA TTCAGTGGCA GCCACCTAAG GAAATGGTTG GGGAGCTGAT GGGCTACCAG 
CTGCAGTACA AGCGCACTGA CGAGGAAAAC TACACCTCCC GCGAGCTGAG AAAGATCGAT 
GACCACTACA CCGTCACCGG GCTGCACAAA GGTGCCACCT ATGTCTTCCG CCTCAGTGCC 
CGCAACCGCG CGGGCATCGG CGAAGCATAC GTGAAAGAGA TCAGCACCCC TGAAGACGTG 
CCGTCGGGCT TCCCGTTCAA CCTGCAGGTC ACGGGACTGA CTCAGTCCAG CACCGAGCTG 
ACCTGGGACC CTCCGCTGGT GGCCGAAAGG AACGGCAAGA TCGTGCACTA TGTGGTCGTT 
TACAGGGACA TCAACAGTCA GCAGAACAGC ACCAACCGGA CGAGCGAAAC GCACATGACC 
ATCCAGGGCC TTAATCCCGA CACCACCTAC GATATCCGGG TGCAGGCGTT CACCAGTAAG 
GGCGGAGGAC CCCTCAGCCC TAGCATCCAG AGCAGGACCA TGTCGAACGA TTTTCCCACC 
CTCAACTTTG GTGTCAAAGC CGTCATGAAA ACATCTGTCC TCCTCACCTG GGATTTGCCA 
CCGAACTACA AATCCCAAGT GCCTTTCAAG ATCCTGTACA ACCAGCAAAG CGTGGAAGTG 
CAGGGATCCC TGAAGAGGAG GCTGATCACG CAGCTGCAGC CAGACACGGA CTACTCTTTT 
GTGCTGACAA GCAGGGGCAA CATTGCTGGT GGTCTACAGC AGCAGGTGTC CATCCGCACC 
GCTCCCGACC TGATCAACGC CAAGCCCAGC ACACATCAGC CTCAGGGTAG CAAGATGACA 
ATCAACCTGC CCAAGGTCCA GACCACCACG CGCGTCAGGT GGTACTACAT CGTGGTGGTG 
CCGGTGTCCC AGTCAACGCA GGGGTGGAAG AATCCAGACG AGATGGACCT GGACGAGCTG 
TTCAAGTCAA GTGAAGGCCC CGTCCTGAGG CGCAGACGTC ACCTGGATTC CTCCAAACCT 
TACATCGCTG CCAAGTTGGC CTCGTTGCCG ACCACCTTCA CCCTGGGTGA TGAGAAGAGA 
TACAACGGCT TCTACAACAA GCCCCTGAGC AGTCCGCAGG AGTATCTCTG CTTTGTCCTG 
GCAGTACTCC GATCCGAAGG GGCAGACAGC ACTACTAACT ATATGACATT TTCAGCCAGT 
CCCTACTCAG ACCCGGTCCA CGTGCGAAAC AGCATGGCTC AGGGGATGGA GCAGCCTGAA 
ATGTTGTGGG TTATGGGCCC TGTTTTGGCC GTGGTCCTGA TTATCATCAT CGTCATCGCC 
ATCCTGCTCT TCAAGAAGAA GCGAGCGTCG CCCCTGCCCA AAGATGAGCA CTCGGGCGGG 
GTGAAGGACT CCCTGCTGGC CAACTCCTCC GACCCTGTGG AGATGAGAAG ACTTAACTAC 
CAGACCCCAG GTCCCAGCTC TCATCGCTGC CCCAACACTC CAAGAATGAG GGAGCACCCG 
CCCATCCCTG TCGTCGACCT GGCTGACCAC ATAGAGCGTC TGAAGGCCAA CGACGGCCTA 
CGCTTTTCCC AGGAGTACGA GTCGATTGAT CCAGGGCAGC AGTTTACCTG GGAGAACTCC 
AACATGGAAG TCAACAAGCC AAAGAATCGC TATGCAAACG TCATTGCCTA TGACCACTCC 
AGAGTGGTTC TCACCTCTGT TGACGCCGTT CCAGGCAGTG ACTACATCAA CGCCAACTAC 
ATCGACGGCT ACAGGAAGCA GAACGCCTAC ATCGCCACGC AGGGGCCACT TCCAGAGACA 
CTGAGCGACT TCTGGAGGAT GGTGTGGGAG CAAAGATCCA ACACCATCGT CATGATGACC 
AGACTGGAGG AGAAGTCACG GGTGAAGTGT GACCAGTACT GGCCGTCGCG AGGAACGGAG 
ACCTATGGGA TGATCCAGGT CACCATGCTG GACACCGTGG AGTTGGCCAC TTACAGCGTC 
CGTACCTTTG CCCTCTATAA GAATGGCTCC AGTGAGAAGA GAGAGGTGAG ACAGTTCCAG 
TTCATGGCCT GGCCAGACCA CGGGGTGCCA GAGTACCCCA CCCCAATCCT GGCCTTCCTA 
CGGCGGGTAA AGGCCTGCAA TCCTCCAGAC GCAGGGCCTA TGGTGGTCCA CTGCAGCGCG 
GGTGTGGGCA GGACCGGATG CTTCATCGTG ATAGATGCCA TGCTGGAGCG CATGAAGCAC 
GAGAAGTCCG TAGACATCTA CGGCCACGTG ACGTGCATGC GCGCCCAGAG GAACTACATG 
GTCCAGACCG AGGACCAGTA CATCTTCATT CACGAAGCCC TGCTGGAGGC CGCCACGTGC 
GGCAACACCG AGGTGCCGGC GCGCAACCTG TACGCCCACA TCCAGAAACT CACCCAGCCC 
CCGCCCGGCG AGACGGTTAC TGCCATGGAG CTGGAATTCA AGAGACTGGC CAACTCCAAA 
GCCCACACAT CCCGCTTCAT CAGCGCCAAC TTGCCCTGCA ACAAGTTCAA GAACCGCCTG 
GTCAACATCA TGCCTTTCGA GTCCACCCGT GTCTGCCTGC AGCCCATCCG TGGCGTCGAG 
GGCTCCGACT ACATCAATGC CAGCTGCATC GACGGCTACA GACAGCAGAA GGCCTACCTG 
GCCACGCAGG GCCCACTGGC AGAGACCACC GAGGACTTCT GGAGGATGCT GTGGGAGCAC 
AACTCCACCA TCGTCGTCAT GCTCACCAAG CTACGAGAGA TGGGCCGGGA GAAGTGCCAT 
CAGTACTGGC CAGCAGAGCG TTCAGCCAGG TACCAGTACT TTGTGGTGGA TCCAATGGCT 
GAGTACAACA TGCCCCAGTA CATCCTCAGA GAGTTCAAAG TCACAGATGC CAGGGACGGC 
CAGTCCAGAA CAATCCGCCA ATTCCAGTTC ACTGACTGGC CCGAGCAAGG AGTGCCAAAA 
ACCGGAGAGG GCTTCATTGA TTTTATCGGA CAAGTGCACA AAACCAAGGA ACAGTTCGGA 
CAGGACGGAC CAATCACTGT ACACTGCAGT GCTGGGGTGG GCAGGACCGG AGTCTTCATC 
ACCCTCAGCA TCGTGCTGGA GAGGATGAGG TATGAAGGAG TTGTCGACCT CTTCCAGACA 
GTGAAGACAC TTCGCACCCA GCGGCCTGCC ATGGTGCAGA CCGAGGACCA GTACCAGTTG 
TGCTACAGGG CAGCATTGGA GTACCTGGGG AGCTTTGACC ACTATGCAAC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104931439 ( XM_019263593.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019119138.1 Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X16, mRNA.

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CloneID	OLa84610
Clone ID Related Accession (Same CDS sequence)	XM_019263593.1
Accession Version	XM_019263593.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	receptor-type tyrosine-protein phosphatase F isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017607936.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTTGACATC 
AGCAGCAGCA ACGGACGCAT TAAACAGCTT CGTTCAGGAG CTCTGCAAAT TGAGAACAGC 
GAGGAGTCCG ACCAAGGGAA ATACGAGTGT GTTGCCATGA ACAGCGCTGG GACCCGTTAC 
TCCGCTCCTG CCAATCTCTA CGTACGAGTG CGAAGAGTCC CACCCAGATT CTCCATTCCG 
CCCACCAACC AAGAGGTGAT GCCAGGCGGC AGCGTCAACC TGACATGCGT GGCAGTGGGC 
TCGCCGATGC CCTACGTCAA GTGGACGATG GGCCTGGTGG AGCTGACCAA GGAGGAGGAG 
ATGCCGATGG GACGCAACGT GCTGGAGGTC AACAACATCC GCGAGTCGGC GAACTACACC 
TGCGTGGCCA TCTCCTCTCT GGGCATGATA GAAGCCACCG CTCAGGTCAC CGTCAAAGCT 
TTGCCAAAGC CTCCCGCCTC CCTCATTGTG ACTGAGACCA CAGCCACCAG TGTGACTCTC 
ACCTGGGACT CTGGAAACCC AGAACCTGTC TCCTACTACA TGATCCAATA CAGAGCCAAG 
ACCTCGGACA ACGGCTTCCA GGAAGTGGAA GGTGTGGCAA CTACCCGTTA CAGCATCGGT 
GGACTTAGCC CCTACTCTGA GTATGAGTTT CGTGTCATGG CTGTCAACAA CATCGGCCGG 
GGGCCACCGA GCGACCCCGT GGAGACACGC ACTGGTGAAC AGGCCCCCTC CTCGCCACCT 
CTGCACGTCC AAGCGCGCAT GCTGAGTGCC AGCACAATGC TGGTCCAGTG GGAACCGCCC 
GAGGAACCCA ACGGGCAAAT CAGGGGCTAC CGGGTCTACT ACAGCTCGGA CTTGACGGCT 
CCGCTCAGCG CCTGGCAGAA GCACAACACA GACGACAGCA GCCTGACGAC CATCTCGGGC 
CTGACTCCTG ATATCACCTA CAGCCTCAGG GTGCTGGGCT TCACCTCTGT AGGTGACGGG 
CCACCTTCTG ACATCCTGCA AGTCAAAACC CAACAGGGAG TCCCTGCCCA GCCGTCCAGC 
TTTGAGGCCG AAGCTGAGCT GGACAGTCGC ATCATGCTAA CATGGCTGTG GCCCGTCCAG 
GACCCCATCA CTAAGTATGA GCTGCAGTAC TGGGAGGCCG GCTCAGACAA CAAGTTACAT 
GTGAGCTTCA ACCCAGCGGG CTCCTACGCT GTGGACGGTC TAAAGCCCGA CACCTTGTAC 
CGTTTCTCTC TGGCAGCCCG TTCTGAGATG GGCTTAGGTG TCTACACCCA ACCCATCGAG 
GCTCGCACCG CCCAGTCCAT GCCGGGAGCA CCCCCCAGGA AGCTGGAGGT TGAGGCCATA 
AACTCCACAG CAATCCGGGT CACCTGGAAG CCGCCCCTGC AGGGGAAGCA GCACGGCCAA 
ATCCGGGGCT ATCAAGTCAT CTTCTCTCGG CTTGAAAACG GCGAACCACG AGGCCAGCCT 
AACATCATGG ACGTTGCTTT GCCAGAGGCA CAGTGGAATA TTGAGGAGTC CACCGAGCAT 
GAGGCCATCA TTGCCGGACT GCACTCTGAA ACCACCTACT CGGTCACCGT GGCAGCATAC 
ACCACCAAAG GAGACGGAGC GCGCAGCAAA GCCAAAGTCA TCACCACGAC GGGTGCAGTG 
CCAGGAAAGC CCACCATGAT GATCAGCACC ACTATGGGCA ACACAGCGCT GATTCAGTGG 
CAGCCACCTA AGGAAATGGT TGGGGAGCTG ATGGGCTACC AGCTGCAGTA CAAGCGCACT 
GACGAGGAAA ACTACACCTC CCGCGAGCTG AGAAAGATCG ATGACCACTA CACCGTCACC 
GGGCTGCACA AAGGTGCCAC CTATGTCTTC CGCCTCAGTG CCCGCAACCG CGCGGGCATC 
GGCGAAGCAT ACGTGAAAGA GATCAGCACC CCTGAAGACG TGCCGTCGGG CTTCCCGTTC 
AACCTGCAGG TCACGGGACT GACTCAGTCC AGCACCGAGC TGACCTGGGA CCCTCCGCTG 
GTGGCCGAAA GGAACGGCAA GATCGTGCAC TATGTGGTCG TTTACAGGGA CATCAACAGT 
CAGCAGAACA GCACCAACCG GACGAGCGAA ACGCACATGA CCATCCAGGG CCTTAATCCC 
GACACCACCT ACGATATCCG GGTGCAGGCG TTCACCAGTA AGGGCGGAGG ACCCCTCAGC 
CCTAGCATCC AGAGCAGGAC CATGTCGAAC GATTTTCCCA CCCTCAACTT TGGTGTCAAA 
GCCGTCATGA AAACATCTGT CCTCCTCACC TGGGATTTGC CACCGAACTA CAAATCCCAA 
GTGCCTTTCA AGATCCTGTA CAACCAGCAA AGCGTGGAAG TGCAGGGATC CCTGAAGAGG 
AGGCTGATCA CGCAGCTGCA GCCAGACACG GACTACTCTT TTGTGCTGAC AAGCAGGGGC 
AACATTGCTG GTGGTCTACA GCAGCAGGTG TCCATCCGCA CCGCTCCCGA CCTGATCAAC 
GCCAAGCCCA GCACACATCA GCCTCAGGGT AGCAAGATGA CAATCAACCT GCCCAAGGTC 
CAGACCACCA CGCGCGTCAG GTGGTACTAC ATCGTGGTGG TGCCGGTGTC CCAGTCAACG 
CAGGGGTGGA AGAATCCAGA CGAGATGGAC CTGGACGAGC TGTTCAAGTC AAGTGAAGGC 
CCCGTCCTGA GGCGCAGACG TCACCTGGAT TCCTCCAAAC CTTACATCGC TGCCAAGTTG 
GCCTCGTTGC CGACCACCTT CACCCTGGGT GATGAGAAGA GATACAACGG CTTCTACAAC 
AAGCCCCTGA GCAGTCCGCA GGAGTATCTC TGCTTTGTCC TGGCAGTACT CCGATCCGAA 
GGGGCAGACA GCACTACTAA CTATATGACA TTTTCAGCCA GTCCCTACTC AGACCCGGTC 
CACGTGCGAA ACAGCATGGC TCAGGGGATG GAGCAGCCTG AAATGTTGTG GGTTATGGGC 
CCTGTTTTGG CCGTGGTCCT GATTATCATC ATCGTCATCG CCATCCTGCT CTTCAAGAAG 
AAGCGAGCGT CGCCCCTGCC CAAAGATGAG CACTCGGGCG GGGTGAAGGA CTCCCTGCTG 
GCCAACTCCT CCGACCCTGT GGAGATGAGA AGACTTAACT ACCAGACCCC AGGTCCCAGC 
TCTCATCGCT GCCCCAACAC TCCAAGAATG AGGGAGCACC CGCCCATCCC TGTCGTCGAC 
CTGGCTGACC ACATAGAGCG TCTGAAGGCC AACGACGGCC TACGCTTTTC CCAGGAGTAC 
GAGTCGATTG ATCCAGGGCA GCAGTTTACC TGGGAGAACT CCAACATGGA AGTCAACAAG 
CCAAAGAATC GCTATGCAAA CGTCATTGCC TATGACCACT CCAGAGTGGT TCTCACCTCT 
GTTGACGCCG TTCCAGGCAG TGACTACATC AACGCCAACT ACATCGACGG CTACAGGAAG 
CAGAACGCCT ACATCGCCAC GCAGGGGCCA CTTCCAGAGA CACTGAGCGA CTTCTGGAGG 
ATGGTGTGGG AGCAAAGATC CAACACCATC GTCATGATGA CCAGACTGGA GGAGAAGTCA 
CGGGTGAAGT GTGACCAGTA CTGGCCGTCG CGAGGAACGG AGACCTATGG GATGATCCAG 
GTCACCATGC TGGACACCGT GGAGTTGGCC ACTTACAGCG TCCGTACCTT TGCCCTCTAT 
AAGAATGGCT CCAGTGAGAA GAGAGAGGTG AGACAGTTCC AGTTCATGGC CTGGCCAGAC 
CACGGGGTGC CAGAGTACCC CACCCCAATC CTGGCCTTCC TACGGCGGGT AAAGGCCTGC 
AATCCTCCAG ACGCAGGGCC TATGGTGGTC CACTGCAGCG CGGGTGTGGG CAGGACCGGA 
TGCTTCATCG TGATAGATGC CATGCTGGAG CGCATGAAGC ACGAGAAGTC CGTAGACATC 
TACGGCCACG TGACGTGCAT GCGCGCCCAG AGGAACTACA TGGTCCAGAC CGAGGACCAG 
TACATCTTCA TTCACGAAGC CCTGCTGGAG GCCGCCACGT GCGGCAACAC CGAGGTGCCG 
GCGCGCAACC TGTACGCCCA CATCCAGAAA CTCACCCAGC CCCCGCCCGG CGAGACGGTT 
ACTGCCATGG AGCTGGAATT CAAGAGACTG GCCAACTCCA AAGCCCACAC ATCCCGCTTC 
ATCAGCGCCA ACTTGCCCTG CAACAAGTTC AAGAACCGCC TGGTCAACAT CATGCCTTTC 
GAGTCCACCC GTGTCTGCCT GCAGCCCATC CGTGGCGTCG AGGGCTCCGA CTACATCAAT 
GCCAGCTGCA TCGACGGCTA CAGACAGCAG AAGGCCTACC TGGCCACGCA GGGCCCACTG 
GCAGAGACCA CCGAGGACTT CTGGAGGATG CTGTGGGAGC ACAACTCCAC CATCGTCGTC 
ATGCTCACCA AGCTACGAGA GATGGGCCGG GAGAAGTGCC ATCAGTACTG GCCAGCAGAG 
CGTTCAGCCA GGTACCAGTA CTTTGTGGTG GATCCAATGG CTGAGTACAA CATGCCCCAG 
TACATCCTCA GAGAGTTCAA AGTCACAGAT GCCAGGGACG GCCAGTCCAG AACAATCCGC 
CAATTCCAGT TCACTGACTG GCCCGAGCAA GGAGTGCCAA AAACCGGAGA GGGCTTCATT 
GATTTTATCG GACAAGTGCA CAAAACCAAG GAACAGTTCG GACAGGACGG ACCAATCACT 
GTACACTGCA GTGCTGGGGT GGGCAGGACC GGAGTCTTCA TCACCCTCAG CATCGTGCTG 
GAGAGGATGA GGTATGAAGG AGTTGTCGAC CTCTTCCAGA CAGTGAAGAC ACTTCGCACC 
CAGCGGCCTG CCATGGTGCA GACCGAGGAC CAGTACCAGT TGTGCTACAG GGCAGCATTG 
GAGTACCTGG GGAGCTTTGA CCACTATGCA ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019119138.1
CDS	521..5956
Translation	MVPNIHLAVPFLPRGALLFLILLSCVVFSSLADSMPSFVKSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVEKTRTATMLCAASGNPDPEISWFKDMLPVDISSSNGRIKQLRSGALQIENSEESDQGKYECVAMNSAGTRYSAPANLYVRVRRVPPRFSIPPTNQEVMPGGSVNLTCVAVGSPMPYVKWTMGLVELTKEEEMPMGRNVLEVNNIRESANYTCVAISSLGMIEATAQVTVKALPKPPASLIVTETTATSVTLTWDSGNPEPVSYYMIQYRAKTSDNGFQEVEGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSDPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRVYYSSDLTAPLSAWQKHNTDDSSLTTISGLTPDITYSLRVLGFTSVGDGPPSDILQVKTQQGVPAQPSSFEAEAELDSRIMLTWLWPVQDPITKYELQYWEAGSDNKLHVSFNPAGSYAVDGLKPDTLYRFSLAARSEMGLGVYTQPIEARTAQSMPGAPPRKLEVEAINSTAIRVTWKPPLQGKQHGQIRGYQVIFSRLENGEPRGQPNIMDVALPEAQWNIEESTEHEAIIAGLHSETTYSVTVAAYTTKGDGARSKAKVITTTGAVPGKPTMMISTTMGNTALIQWQPPKEMVGELMGYQLQYKRTDEENYTSRELRKIDDHYTVTGLHKGATYVFRLSARNRAGIGEAYVKEISTPEDVPSGFPFNLQVTGLTQSSTELTWDPPLVAERNGKIVHYVVVYRDINSQQNSTNRTSETHMTIQGLNPDTTYDIRVQAFTSKGGGPLSPSIQSRTMSNDFPTLNFGVKAVMKTSVLLTWDLPPNYKSQVPFKILYNQQSVEVQGSLKRRLITQLQPDTDYSFVLTSRGNIAGGLQQQVSIRTAPDLINAKPSTHQPQGSKMTINLPKVQTTTRVRWYYIVVVPVSQSTQGWKNPDEMDLDELFKSSEGPVLRRRRHLDSSKPYIAAKLASLPTTFTLGDEKRYNGFYNKPLSSPQEYLCFVLAVLRSEGADSTTNYMTFSASPYSDPVHVRNSMAQGMEQPEMLWVMGPVLAVVLIIIIVIAILLFKKKRASPLPKDEHSGGVKDSLLANSSDPVEMRRLNYQTPGPSSHRCPNTPRMREHPPIPVVDLADHIERLKANDGLRFSQEYESIDPGQQFTWENSNMEVNKPKNRYANVIAYDHSRVVLTSVDAVPGSDYINANYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRSNTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVTMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRAQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPPPGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYLATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_019263593.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea receptor-type tyrosine-protein phosphatase F (LOC104931439), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019263593.1

ORF Insert Sequence:

ATGGTGCCTA ATATTCACTT GGCAGTGCCC TTCCTGCCCA GAGGGGCATT GCTGTTCCTG 
ATTCTCCTCA GCTGTGTGGT GTTCTCCTCG CTGGCAGACA GCATGCCAAG TTTCGTCAAA 
TCCCCAGAGG ACCAGACGGG CATCTCGGGC GGAGTCGCGT CGTTTGTGTG CCAGGCCGCC 
GGGGAGCCCA AACCCAGAAT CACCTGGATG AAGAAAGGCA AGAAAGTCAG CTCCCAGCGC 
TTTGAGGTGA TTGAGTTTGA TGATGGCTCG GGCTCCGTAC TGCGGATCCA ACCACTGCGC 
ACACACAGAG ACGAAGCCAT TTACGAGTGC ACGGCCACCA ACAGTGTTGG CGAGATCAAC 
ACCAGTGCCA AGCTTACAGT GCTTGAAGAG AACCAGATCC CCCATGGTTT CCCAACCATC 
GATATGGGTC CCCAGCTGAA GGTAGTTGAA AAGACCAGAA CTGCCACCAT GCTGTGCGCC 
GCCAGCGGAA ACCCGGATCC AGAGATCTCC TGGTTCAAGG ACATGCTTCC CGTTGACATC 
AGCAGCAGCA ACGGACGCAT TAAACAGCTT CGTTCAGGAG CTCTGCAAAT TGAGAACAGC 
GAGGAGTCCG ACCAAGGGAA ATACGAGTGT GTTGCCATGA ACAGCGCTGG GACCCGTTAC 
TCCGCTCCTG CCAATCTCTA CGTACGAGTG CGAAGAGTCC CACCCAGATT CTCCATTCCG 
CCCACCAACC AAGAGGTGAT GCCAGGCGGC AGCGTCAACC TGACATGCGT GGCAGTGGGC 
TCGCCGATGC CCTACGTCAA GTGGACGATG GGCCTGGTGG AGCTGACCAA GGAGGAGGAG 
ATGCCGATGG GACGCAACGT GCTGGAGGTC AACAACATCC GCGAGTCGGC GAACTACACC 
TGCGTGGCCA TCTCCTCTCT GGGCATGATA GAAGCCACCG CTCAGGTCAC CGTCAAAGCT 
TTGCCAAAGC CTCCCGCCTC CCTCATTGTG ACTGAGACCA CAGCCACCAG TGTGACTCTC 
ACCTGGGACT CTGGAAACCC AGAACCTGTC TCCTACTACA TGATCCAATA CAGAGCCAAG 
ACCTCGGACA ACGGCTTCCA GGAAGTGGAA GGTGTGGCAA CTACCCGTTA CAGCATCGGT 
GGACTTAGCC CCTACTCTGA GTATGAGTTT CGTGTCATGG CTGTCAACAA CATCGGCCGG 
GGGCCACCGA GCGACCCCGT GGAGACACGC ACTGGTGAAC AGGCCCCCTC CTCGCCACCT 
CTGCACGTCC AAGCGCGCAT GCTGAGTGCC AGCACAATGC TGGTCCAGTG GGAACCGCCC 
GAGGAACCCA ACGGGCAAAT CAGGGGCTAC CGGGTCTACT ACAGCTCGGA CTTGACGGCT 
CCGCTCAGCG CCTGGCAGAA GCACAACACA GACGACAGCA GCCTGACGAC CATCTCGGGC 
CTGACTCCTG ATATCACCTA CAGCCTCAGG GTGCTGGGCT TCACCTCTGT AGGTGACGGG 
CCACCTTCTG ACATCCTGCA AGTCAAAACC CAACAGGGAG TCCCTGCCCA GCCGTCCAGC 
TTTGAGGCCG AAGCTGAGCT GGACAGTCGC ATCATGCTAA CATGGCTGTG GCCCGTCCAG 
GACCCCATCA CTAAGTATGA GCTGCAGTAC TGGGAGGCCG GCTCAGACAA CAAGTTACAT 
GTGAGCTTCA ACCCAGCGGG CTCCTACGCT GTGGACGGTC TAAAGCCCGA CACCTTGTAC 
CGTTTCTCTC TGGCAGCCCG TTCTGAGATG GGCTTAGGTG TCTACACCCA ACCCATCGAG 
GCTCGCACCG CCCAGTCCAT GCCGGGAGCA CCCCCCAGGA AGCTGGAGGT TGAGGCCATA 
AACTCCACAG CAATCCGGGT CACCTGGAAG CCGCCCCTGC AGGGGAAGCA GCACGGCCAA 
ATCCGGGGCT ATCAAGTCAT CTTCTCTCGG CTTGAAAACG GCGAACCACG AGGCCAGCCT 
AACATCATGG ACGTTGCTTT GCCAGAGGCA CAGTGGAATA TTGAGGAGTC CACCGAGCAT 
GAGGCCATCA TTGCCGGACT GCACTCTGAA ACCACCTACT CGGTCACCGT GGCAGCATAC 
ACCACCAAAG GAGACGGAGC GCGCAGCAAA GCCAAAGTCA TCACCACGAC GGGTGCAGTG 
CCAGGAAAGC CCACCATGAT GATCAGCACC ACTATGGGCA ACACAGCGCT GATTCAGTGG 
CAGCCACCTA AGGAAATGGT TGGGGAGCTG ATGGGCTACC AGCTGCAGTA CAAGCGCACT 
GACGAGGAAA ACTACACCTC CCGCGAGCTG AGAAAGATCG ATGACCACTA CACCGTCACC 
GGGCTGCACA AAGGTGCCAC CTATGTCTTC CGCCTCAGTG CCCGCAACCG CGCGGGCATC 
GGCGAAGCAT ACGTGAAAGA GATCAGCACC CCTGAAGACG TGCCGTCGGG CTTCCCGTTC 
AACCTGCAGG TCACGGGACT GACTCAGTCC AGCACCGAGC TGACCTGGGA CCCTCCGCTG 
GTGGCCGAAA GGAACGGCAA GATCGTGCAC TATGTGGTCG TTTACAGGGA CATCAACAGT 
CAGCAGAACA GCACCAACCG GACGAGCGAA ACGCACATGA CCATCCAGGG CCTTAATCCC 
GACACCACCT ACGATATCCG GGTGCAGGCG TTCACCAGTA AGGGCGGAGG ACCCCTCAGC 
CCTAGCATCC AGAGCAGGAC CATGTCGAAC GATTTTCCCA CCCTCAACTT TGGTGTCAAA 
GCCGTCATGA AAACATCTGT CCTCCTCACC TGGGATTTGC CACCGAACTA CAAATCCCAA 
GTGCCTTTCA AGATCCTGTA CAACCAGCAA AGCGTGGAAG TGCAGGGATC CCTGAAGAGG 
AGGCTGATCA CGCAGCTGCA GCCAGACACG GACTACTCTT TTGTGCTGAC AAGCAGGGGC 
AACATTGCTG GTGGTCTACA GCAGCAGGTG TCCATCCGCA CCGCTCCCGA CCTGATCAAC 
GCCAAGCCCA GCACACATCA GCCTCAGGGT AGCAAGATGA CAATCAACCT GCCCAAGGTC 
CAGACCACCA CGCGCGTCAG GTGGTACTAC ATCGTGGTGG TGCCGGTGTC CCAGTCAACG 
CAGGGGTGGA AGAATCCAGA CGAGATGGAC CTGGACGAGC TGTTCAAGTC AAGTGAAGGC 
CCCGTCCTGA GGCGCAGACG TCACCTGGAT TCCTCCAAAC CTTACATCGC TGCCAAGTTG 
GCCTCGTTGC CGACCACCTT CACCCTGGGT GATGAGAAGA GATACAACGG CTTCTACAAC 
AAGCCCCTGA GCAGTCCGCA GGAGTATCTC TGCTTTGTCC TGGCAGTACT CCGATCCGAA 
GGGGCAGACA GCACTACTAA CTATATGACA TTTTCAGCCA GTCCCTACTC AGACCCGGTC 
CACGTGCGAA ACAGCATGGC TCAGGGGATG GAGCAGCCTG AAATGTTGTG GGTTATGGGC 
CCTGTTTTGG CCGTGGTCCT GATTATCATC ATCGTCATCG CCATCCTGCT CTTCAAGAAG 
AAGCGAGCGT CGCCCCTGCC CAAAGATGAG CACTCGGGCG GGGTGAAGGA CTCCCTGCTG 
GCCAACTCCT CCGACCCTGT GGAGATGAGA AGACTTAACT ACCAGACCCC AGGTCCCAGC 
TCTCATCGCT GCCCCAACAC TCCAAGAATG AGGGAGCACC CGCCCATCCC TGTCGTCGAC 
CTGGCTGACC ACATAGAGCG TCTGAAGGCC AACGACGGCC TACGCTTTTC CCAGGAGTAC 
GAGTCGATTG ATCCAGGGCA GCAGTTTACC TGGGAGAACT CCAACATGGA AGTCAACAAG 
CCAAAGAATC GCTATGCAAA CGTCATTGCC TATGACCACT CCAGAGTGGT TCTCACCTCT 
GTTGACGCCG TTCCAGGCAG TGACTACATC AACGCCAACT ACATCGACGG CTACAGGAAG 
CAGAACGCCT ACATCGCCAC GCAGGGGCCA CTTCCAGAGA CACTGAGCGA CTTCTGGAGG 
ATGGTGTGGG AGCAAAGATC CAACACCATC GTCATGATGA CCAGACTGGA GGAGAAGTCA 
CGGGTGAAGT GTGACCAGTA CTGGCCGTCG CGAGGAACGG AGACCTATGG GATGATCCAG 
GTCACCATGC TGGACACCGT GGAGTTGGCC ACTTACAGCG TCCGTACCTT TGCCCTCTAT 
AAGAATGGCT CCAGTGAGAA GAGAGAGGTG AGACAGTTCC AGTTCATGGC CTGGCCAGAC 
CACGGGGTGC CAGAGTACCC CACCCCAATC CTGGCCTTCC TACGGCGGGT AAAGGCCTGC 
AATCCTCCAG ACGCAGGGCC TATGGTGGTC CACTGCAGCG CGGGTGTGGG CAGGACCGGA 
TGCTTCATCG TGATAGATGC CATGCTGGAG CGCATGAAGC ACGAGAAGTC CGTAGACATC 
TACGGCCACG TGACGTGCAT GCGCGCCCAG AGGAACTACA TGGTCCAGAC CGAGGACCAG 
TACATCTTCA TTCACGAAGC CCTGCTGGAG GCCGCCACGT GCGGCAACAC CGAGGTGCCG 
GCGCGCAACC TGTACGCCCA CATCCAGAAA CTCACCCAGC CCCCGCCCGG CGAGACGGTT 
ACTGCCATGG AGCTGGAATT CAAGAGACTG GCCAACTCCA AAGCCCACAC ATCCCGCTTC 
ATCAGCGCCA ACTTGCCCTG CAACAAGTTC AAGAACCGCC TGGTCAACAT CATGCCTTTC 
GAGTCCACCC GTGTCTGCCT GCAGCCCATC CGTGGCGTCG AGGGCTCCGA CTACATCAAT 
GCCAGCTGCA TCGACGGCTA CAGACAGCAG AAGGCCTACC TGGCCACGCA GGGCCCACTG 
GCAGAGACCA CCGAGGACTT CTGGAGGATG CTGTGGGAGC ACAACTCCAC CATCGTCGTC 
ATGCTCACCA AGCTACGAGA GATGGGCCGG GAGAAGTGCC ATCAGTACTG GCCAGCAGAG 
CGTTCAGCCA GGTACCAGTA CTTTGTGGTG GATCCAATGG CTGAGTACAA CATGCCCCAG 
TACATCCTCA GAGAGTTCAA AGTCACAGAT GCCAGGGACG GCCAGTCCAG AACAATCCGC 
CAATTCCAGT TCACTGACTG GCCCGAGCAA GGAGTGCCAA AAACCGGAGA GGGCTTCATT 
GATTTTATCG GACAAGTGCA CAAAACCAAG GAACAGTTCG GACAGGACGG ACCAATCACT 
GTACACTGCA GTGCTGGGGT GGGCAGGACC GGAGTCTTCA TCACCCTCAG CATCGTGCTG 
GAGAGGATGA GGTATGAAGG AGTTGTCGAC CTCTTCCAGA CAGTGAAGAC ACTTCGCACC 
CAGCGGCCTG CCATGGTGCA GACCGAGGAC CAGTACCAGT TGTGCTACAG GGCAGCATTG 
GAGTACCTGG GGAGCTTTGA CCACTATGCA ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

LOC104931439 cDNA ORF clone, Larimichthys crocea(large yellow croaker)

Gene

Clones

gRNAs

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