LOC104930715 cDNA ORF clone, Larimichthys crocea(large yellow croaker)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC104930715
Entrez Gene ID	104930715
Full Name	histone-lysine N-methyltransferase KMT5B-like
Gene Type	protein-coding
Organism	Larimichthys crocea(large yellow croaker)

mRNA and Protein(s)

mRNA	Protein	Name
XM_027285559.1	XP_027141360.1	uncharacterized protein LOC104930715 isoform X1
XM_019256099.1	XP_019111644.1	uncharacterized protein LOC104930715 isoform X1
XM_019256105.1	XP_019111650.1	uncharacterized protein LOC104930715 isoform X2
XM_019256109.1	XP_019111654.1	histone-lysine N-methyltransferase KMT5B-like isoform X3
XM_027285560.1	XP_027141361.1	uncharacterized protein LOC104930715 isoform X2
XM_027285561.1	XP_027141362.1	histone-lysine N-methyltransferase KMT5B isoform X3

Pathways from BioSystems
Gene Ontology

The following LOC104930715 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC104930715 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OLa202057	XM_027285561.1 Latest version!	Larimichthys crocea histone-lysine N-methyltransferase KMT5B (LOC104930715), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OLa86932	XM_019256109.1 Latest version!	Larimichthys crocea histone-lysine N-methyltransferase KMT5B-like (LOC104930715), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OLa202055	XM_027285559.1 Latest version!	Larimichthys crocea histone-lysine N-methyltransferase KMT5B (LOC104930715), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OLa202056	XM_027285560.1 Latest version!	Larimichthys crocea histone-lysine N-methyltransferase KMT5B (LOC104930715), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OLa86930	XM_019256099.1 Latest version!	Larimichthys crocea histone-lysine N-methyltransferase KMT5B-like (LOC104930715), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OLa86931	XM_019256105.1 Latest version!	Larimichthys crocea histone-lysine N-methyltransferase KMT5B-like (LOC104930715), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC104930715 ( XM_027285561.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_027141362.1 Larimichthys crocea histone-lysine N-methyltransferase KMT5B (LOC104930715), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OLa202057
Clone ID Related Accession (Same CDS sequence)	XM_027285561.1
Accession Version	XM_027285561.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	histone-lysine N-methyltransferase KMT5B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040022.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGGAT GCACTAGAAT GAGTGTGAAG GAGCTGTGTG AGACCGATGA CCTGGCCACC 
GGTTTGGTGC TGGACCCCCT GCTGGGCTTC AGCACCCACA AAATGAACAT CAGCCCCCCT 
CCTGAGATCC GGCGTTGGGG TAATCTCAAA GAGACCCTGT TGCGCTTTCA GCGTACGCAT 
GACTTCAATG CTACGTTTGA GGCGCTGACG GTGGGCGAGC TGGCTGGTGA TTACTTCAGT 
GCTTTGGGAA GTCACCGTCA GGAGCTGCTG AGGCAACATG TGTATCGCTA CCTCAGTGCC 
TTCCTGTTGG ATAGCGGTAT CAAGATCGAG TCCTGTGACC GATACTCCTC AGAGACCAAT 
GGAGCAAAGA TAACCTCAAC AAGGCACTGG TTTGCAGGAG AGCGGGTGGA GGTCCTGCTG 
GGCTGCATAG CAGAGCTGAG CCCCGCTGAC AGTGCCGTGC TGAGGACGGG AGTCAATGAC 
TTCAGTGTGA TGTATTCCAC GCGCAAACGC TGCGCTCAGC TCTGGCTCGG GCCTGCAGCT 
TTCATTAACC ATGACTGCAA ACCAAACTGC AAGTTCGTCC CTGGTGAGAA GAATATCGCT 
TGTGTTGAAG CCATTCGACC TATCTCACCT GGGGAGGAGA TCACATGTTA CTATGGTGCC 
AGCTTTTTTG GGGAAGGAAA TGAAATGTGT GAATGCTGCA CCTGTGAGAG GAACGGAGAG 
GGCCACTTCA AACACAGAGG GAAGCAGCCT GAATGTGAAG AAACAAAGGA CCCCGTGGGC 
CAAAAGTACC GTCTGAGAGA GCGATATCTT CGTCATCACA GAGAGAAGGG TCACTTTCCT 
ACACGGCAGA TAATACCAGG ATTACACTCA GGTATCTTTA AAGCCATCCC CTCAAGGAAT 
TCCTTTACCC AGCAGATGAG GAGAAATGCC TTGAAGAACA GAAAATTTAG CAAATCAAAG 
AAGTGGAGAC GGGAGCACAG ACGATCAGGA AAGCACCCGC TGACATCCTC CACGGCCAAC 
CAGTGTCGGA CAGTTCCACT TTTATCCCAA GTTACTCTGA GGGACCTCAG AATCAGAGTA 
CGGCGCCACT CAGTGGATTT CTTACTCCAT TGTAAGGATC CTAAAAGCAA AGAAAGGGCT 
CTGCTTCAAC AACTGGAGGA AGTGAAGCCC CTGAGTGAAC TGAATACGAC TGGTGAAGGG 
AATAACAACA GTGAGGTAAA CTTGAAGCCC TTCACTCTAA ATTCCCCCAT GTCTGTTCAT 
CAGAAAAGTA GACCTGCAGG AGGCAGCGCA GGCCTTAATG GAAAGTCTGT TAAAGTGGTG 
GATAGGCAGG TGGCTCTGAG GACATCTTCG AGAACAAGAA GCATGGTTCA CAACACGGTG 
ACACATCAAA CAGTGTCCAG CAACACAGCC AACACTAAGA CTGTTTGTCA CCCTGTTCAA 
ATTGACAGAA CCTGCAATGC AATAACAAGC GAGAAGACTG AACAAGCAAT GACCGAGAAT 
CAAAGCAACA AGTGCAGCAG CGGCGTTGAA TCCTGCATCA TCGACGTGGA TCAAAATCCT 
GCTGAGCAAA CCGAGCACAA GACCAGCGTA GCGTCATCCG TGTTAGAAGT TTGTCAGAGT 
GTCACAGATA GATCGAAGAA AGCCGGAGAC AATCCATTAA TATCACTGAA GCAATACCTT 
ACAGTCAACC TAATAAGGTT ATCAGTGCCA CAGAGTCTAG AAGCAGAAAA GACTCCTGAA 
AACAAGAGAG GAAGCGTGGT GCTAAGAGGA AGGAGCAGGC AGAGTCTTAG ACCAGCGGCC 
ATGACAGCAC AGTCAGAAAG AAGAGTGTCC AGATCTCAGC AGAGGCAGGG AGGAAGTTCA 
GAGATCTGCA CATCTGAAAG CAACAGAGGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027141362.1
CDS	390..2282
Translation	MDGCTRMSVKELCETDDLATGLVLDPLLGFSTHKMNISPPPEIRRWGNLKETLLRFQRTHDFNATFEALTVGELAGDYFSALGSHRQELLRQHVYRYLSAFLLDSGIKIESCDRYSSETNGAKITSTRHWFAGERVEVLLGCIAELSPADSAVLRTGVNDFSVMYSTRKRCAQLWLGPAAFINHDCKPNCKFVPGEKNIACVEAIRPISPGEEITCYYGASFFGEGNEMCECCTCERNGEGHFKHRGKQPECEETKDPVGQKYRLRERYLRHHREKGHFPTRQIIPGLHSGIFKAIPSRNSFTQQMRRNALKNRKFSKSKKWRREHRRSGKHPLTSSTANQCRTVPLLSQVTLRDLRIRVRRHSVDFLLHCKDPKSKERALLQQLEEVKPLSELNTTGEGNNNSEVNLKPFTLNSPMSVHQKSRPAGGSAGLNGKSVKVVDRQVALRTSSRTRSMVHNTVTHQTVSSNTANTKTVCHPVQIDRTCNAITSEKTEQAMTENQSNKCSSGVESCIIDVDQNPAEQTEHKTSVASSVLEVCQSVTDRSKKAGDNPLISLKQYLTVNLIRLSVPQSLEAEKTPENKRGSVVLRGRSRQSLRPAAMTAQSERRVSRSQQRQGGSSEICTSESNRG

Target ORF information:

RefSeq Version	XM_027285561.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea histone-lysine N-methyltransferase KMT5B (LOC104930715), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027285561.1

ORF Insert Sequence:

ATGGACGGAT GCACTAGAAT GAGTGTGAAG GAGCTGTGTG AGACCGATGA CCTGGCCACC 
GGTTTGGTGC TGGACCCCCT GCTGGGCTTC AGCACCCACA AAATGAACAT CAGCCCCCCT 
CCTGAGATCC GGCGTTGGGG TAATCTCAAA GAGACCCTGT TGCGCTTTCA GCGTACGCAT 
GACTTCAATG CTACGTTTGA GGCGCTGACG GTGGGCGAGC TGGCTGGTGA TTACTTCAGT 
GCTTTGGGAA GTCACCGTCA GGAGCTGCTG AGGCAACATG TGTATCGCTA CCTCAGTGCC 
TTCCTGTTGG ATAGCGGTAT CAAGATCGAG TCCTGTGACC GATACTCCTC AGAGACCAAT 
GGAGCAAAGA TAACCTCAAC AAGGCACTGG TTTGCAGGAG AGCGGGTGGA GGTCCTGCTG 
GGCTGCATAG CAGAGCTGAG CCCCGCTGAC AGTGCCGTGC TGAGGACGGG AGTCAATGAC 
TTCAGTGTGA TGTATTCCAC GCGCAAACGC TGCGCTCAGC TCTGGCTCGG GCCTGCAGCT 
TTCATTAACC ATGACTGCAA ACCAAACTGC AAGTTCGTCC CTGGTGAGAA GAATATCGCT 
TGTGTTGAAG CCATTCGACC TATCTCACCT GGGGAGGAGA TCACATGTTA CTATGGTGCC 
AGCTTTTTTG GGGAAGGAAA TGAAATGTGT GAATGCTGCA CCTGTGAGAG GAACGGAGAG 
GGCCACTTCA AACACAGAGG GAAGCAGCCT GAATGTGAAG AAACAAAGGA CCCCGTGGGC 
CAAAAGTACC GTCTGAGAGA GCGATATCTT CGTCATCACA GAGAGAAGGG TCACTTTCCT 
ACACGGCAGA TAATACCAGG ATTACACTCA GGTATCTTTA AAGCCATCCC CTCAAGGAAT 
TCCTTTACCC AGCAGATGAG GAGAAATGCC TTGAAGAACA GAAAATTTAG CAAATCAAAG 
AAGTGGAGAC GGGAGCACAG ACGATCAGGA AAGCACCCGC TGACATCCTC CACGGCCAAC 
CAGTGTCGGA CAGTTCCACT TTTATCCCAA GTTACTCTGA GGGACCTCAG AATCAGAGTA 
CGGCGCCACT CAGTGGATTT CTTACTCCAT TGTAAGGATC CTAAAAGCAA AGAAAGGGCT 
CTGCTTCAAC AACTGGAGGA AGTGAAGCCC CTGAGTGAAC TGAATACGAC TGGTGAAGGG 
AATAACAACA GTGAGGTAAA CTTGAAGCCC TTCACTCTAA ATTCCCCCAT GTCTGTTCAT 
CAGAAAAGTA GACCTGCAGG AGGCAGCGCA GGCCTTAATG GAAAGTCTGT TAAAGTGGTG 
GATAGGCAGG TGGCTCTGAG GACATCTTCG AGAACAAGAA GCATGGTTCA CAACACGGTG 
ACACATCAAA CAGTGTCCAG CAACACAGCC AACACTAAGA CTGTTTGTCA CCCTGTTCAA 
ATTGACAGAA CCTGCAATGC AATAACAAGC GAGAAGACTG AACAAGCAAT GACCGAGAAT 
CAAAGCAACA AGTGCAGCAG CGGCGTTGAA TCCTGCATCA TCGACGTGGA TCAAAATCCT 
GCTGAGCAAA CCGAGCACAA GACCAGCGTA GCGTCATCCG TGTTAGAAGT TTGTCAGAGT 
GTCACAGATA GATCGAAGAA AGCCGGAGAC AATCCATTAA TATCACTGAA GCAATACCTT 
ACAGTCAACC TAATAAGGTT ATCAGTGCCA CAGAGTCTAG AAGCAGAAAA GACTCCTGAA 
AACAAGAGAG GAAGCGTGGT GCTAAGAGGA AGGAGCAGGC AGAGTCTTAG ACCAGCGGCC 
ATGACAGCAC AGTCAGAAAG AAGAGTGTCC AGATCTCAGC AGAGGCAGGG AGGAAGTTCA 
GAGATCTGCA CATCTGAAAG CAACAGAGGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104930715 ( XM_019256109.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019111654.1 Larimichthys crocea histone-lysine N-methyltransferase KMT5B-like (LOC104930715), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OLa86932
Clone ID Related Accession (Same CDS sequence)	XM_019256109.1
Accession Version	XM_019256109.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	histone-lysine N-methyltransferase KMT5B-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017608059.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGGAT GCACTAGAAT GAGTGTGAAG GAGCTGTGTG AGACCGATGA CCTGGCCACC 
GGTTTGGTGC TGGACCCCCT GCTGGGCTTC AGCACCCACA AAATGAACAT CAGCCCCCCT 
CCTGAGATCC GGCGTTGGGG TAATCTCAAA GAGACCCTGT TGCGCTTTCA GCGTACGCAT 
GACTTCAATG CTACGTTTGA GGCGCTGACG GTGGGCGAGC TGGCTGGTGA TTACTTCAGT 
GCTTTGGGAA GTCACCGTCA GGAGCTGCTG AGGCAACATG TGTATCGCTA CCTCAGTGCC 
TTCCTGTTGG ATAGCGGTAT CAAGATAGAG TCCTGTGACC GATACTCCTC AGAGACCAAT 
GGAGCAAAGA TAACCTCAAC AAGGCACTGG TTTGCAGGAG AGCGGGTGGA GGTCCTGCTG 
GGCTGCATAG CAGAGCTGAG CCCCGCTGAC AGTGCCGTGC TGAGGACGGG AGTCAATGAC 
TTCAGTGTGA TGTATTCCAC GCGCAAACGC TGCGCTCAGC TCTGGCTCGG GCCTGCAGCT 
TTCATTAACC ATGACTGCAA ACCAAACTGC AAGTTCGTCC CTGGTGAGAA GAATATCGCT 
TGTGTTGAAG CCATTCGACC TATCTCACCT GGGGAGGAGA TCACATGTTA CTATGGTGCC 
AGCTTTTTTG GGGAAGGAAA TGAAATGTGT GAATGCTGCA CCTGTGAGAG GAACGGAGAG 
GGCCACTTCA AACACAGAGG GAAGCAGCCT GAATGTGAAG AAACAAAGGA CCCCGTGGGC 
CAAAAGTACC GTCTGAGAGA GCGATATCTT CGTCATCACA GAGAGAAGGG TCACTTTCCT 
ACACGGCAGA TAATACCAGG ATTACACTCA GGTATCTTTA AAGCCATCCC CTCAAGGAAT 
TCCTTTACCC AGCAGATGAG GAGAAATGCC TTGAAGAACA GAAAATTTAG CAAATCAAAG 
AAGTGGAGAC GGGAGCACAG ACGATCAGGA AAGCACCCGC TGACATCCTC CACGGCCAAC 
CAGTGTCGGA CAGTTCCACT TTTATCCCAA GTTACTCTGA GGGACCTCAG AATCAGAGTA 
CGGCGCCACT CAGTGGATTT CTTACTCCAT TGTAAGGATC CTAAAAGCAA AGAAAGGGCT 
CTGCTTCAAC AACTGGAGGA AGTGAAGCCC CTGAGTGAAC TGAATACGAC TGGTGAAGGG 
AATAACAACA GTGAGGTAAA CTTGAAGCCC TTCACTCTAA ATTCCCCCAT GCCTGTTCAT 
CAGAAAAGTA GACATGCAGG AGGCAGCGCA GGCCTTAATG GAAAGTCTGT TAAAGTGGTG 
GATAGGCAGG TGGCTCTGAG GACATCTTCG AGAACAAGAA GCATGGTTCA CAACACGGTG 
ACACATCAAA CAGTGTCCAG CAACACAGCC AACACTAAGA CTGTTTGTCA CCCTGTTCAA 
ATTGACAGAA CCTGCAATGC AATAACAAGC GAGAAGACTG AACAAGCAAT GACCGAGAAT 
CAAAGCAACA AGTGCAGCAG CGGCGTTGAA TCCTGCATCA TCGACGTGGA TCAAAATCCT 
GCTGAGCAAA CCGAGCACAA GACCAGCGTA GCGTCATCCG TGTTAGAAGT TTGTCAGAGT 
GTCACAGATA GATCGAAGAA AGCCGGAGAC AATCCATTAA TATCACTGAA GCAATACCTT 
ACAGTCAACC TAATAAGGTT ATCAGTGCCA CAGAGTCTAG AAGCAGAAAA GACTCCTGAA 
AACAAGAGAG GAAGCGTGGT GCTAAGAGGA AGGAGCAGGC AGAGTCTTAG ACCAGCGGCC 
ATGACAGCAC AGTCAGAAAG AAGAGTGTCC AGATCTCAGC AGAGGCAGGG AGGAAGTTCA 
GAGATCTGCA CATCTGAAAG CAACAGAGGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019111654.1
CDS	423..2315
Translation	MDGCTRMSVKELCETDDLATGLVLDPLLGFSTHKMNISPPPEIRRWGNLKETLLRFQRTHDFNATFEALTVGELAGDYFSALGSHRQELLRQHVYRYLSAFLLDSGIKIESCDRYSSETNGAKITSTRHWFAGERVEVLLGCIAELSPADSAVLRTGVNDFSVMYSTRKRCAQLWLGPAAFINHDCKPNCKFVPGEKNIACVEAIRPISPGEEITCYYGASFFGEGNEMCECCTCERNGEGHFKHRGKQPECEETKDPVGQKYRLRERYLRHHREKGHFPTRQIIPGLHSGIFKAIPSRNSFTQQMRRNALKNRKFSKSKKWRREHRRSGKHPLTSSTANQCRTVPLLSQVTLRDLRIRVRRHSVDFLLHCKDPKSKERALLQQLEEVKPLSELNTTGEGNNNSEVNLKPFTLNSPMPVHQKSRHAGGSAGLNGKSVKVVDRQVALRTSSRTRSMVHNTVTHQTVSSNTANTKTVCHPVQIDRTCNAITSEKTEQAMTENQSNKCSSGVESCIIDVDQNPAEQTEHKTSVASSVLEVCQSVTDRSKKAGDNPLISLKQYLTVNLIRLSVPQSLEAEKTPENKRGSVVLRGRSRQSLRPAAMTAQSERRVSRSQQRQGGSSEICTSESNRG

Target ORF information:

RefSeq Version	XM_019256109.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea histone-lysine N-methyltransferase KMT5B-like (LOC104930715), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019256109.1

ORF Insert Sequence:

ATGGACGGAT GCACTAGAAT GAGTGTGAAG GAGCTGTGTG AGACCGATGA CCTGGCCACC 
GGTTTGGTGC TGGACCCCCT GCTGGGCTTC AGCACCCACA AAATGAACAT CAGCCCCCCT 
CCTGAGATCC GGCGTTGGGG TAATCTCAAA GAGACCCTGT TGCGCTTTCA GCGTACGCAT 
GACTTCAATG CTACGTTTGA GGCGCTGACG GTGGGCGAGC TGGCTGGTGA TTACTTCAGT 
GCTTTGGGAA GTCACCGTCA GGAGCTGCTG AGGCAACATG TGTATCGCTA CCTCAGTGCC 
TTCCTGTTGG ATAGCGGTAT CAAGATAGAG TCCTGTGACC GATACTCCTC AGAGACCAAT 
GGAGCAAAGA TAACCTCAAC AAGGCACTGG TTTGCAGGAG AGCGGGTGGA GGTCCTGCTG 
GGCTGCATAG CAGAGCTGAG CCCCGCTGAC AGTGCCGTGC TGAGGACGGG AGTCAATGAC 
TTCAGTGTGA TGTATTCCAC GCGCAAACGC TGCGCTCAGC TCTGGCTCGG GCCTGCAGCT 
TTCATTAACC ATGACTGCAA ACCAAACTGC AAGTTCGTCC CTGGTGAGAA GAATATCGCT 
TGTGTTGAAG CCATTCGACC TATCTCACCT GGGGAGGAGA TCACATGTTA CTATGGTGCC 
AGCTTTTTTG GGGAAGGAAA TGAAATGTGT GAATGCTGCA CCTGTGAGAG GAACGGAGAG 
GGCCACTTCA AACACAGAGG GAAGCAGCCT GAATGTGAAG AAACAAAGGA CCCCGTGGGC 
CAAAAGTACC GTCTGAGAGA GCGATATCTT CGTCATCACA GAGAGAAGGG TCACTTTCCT 
ACACGGCAGA TAATACCAGG ATTACACTCA GGTATCTTTA AAGCCATCCC CTCAAGGAAT 
TCCTTTACCC AGCAGATGAG GAGAAATGCC TTGAAGAACA GAAAATTTAG CAAATCAAAG 
AAGTGGAGAC GGGAGCACAG ACGATCAGGA AAGCACCCGC TGACATCCTC CACGGCCAAC 
CAGTGTCGGA CAGTTCCACT TTTATCCCAA GTTACTCTGA GGGACCTCAG AATCAGAGTA 
CGGCGCCACT CAGTGGATTT CTTACTCCAT TGTAAGGATC CTAAAAGCAA AGAAAGGGCT 
CTGCTTCAAC AACTGGAGGA AGTGAAGCCC CTGAGTGAAC TGAATACGAC TGGTGAAGGG 
AATAACAACA GTGAGGTAAA CTTGAAGCCC TTCACTCTAA ATTCCCCCAT GCCTGTTCAT 
CAGAAAAGTA GACATGCAGG AGGCAGCGCA GGCCTTAATG GAAAGTCTGT TAAAGTGGTG 
GATAGGCAGG TGGCTCTGAG GACATCTTCG AGAACAAGAA GCATGGTTCA CAACACGGTG 
ACACATCAAA CAGTGTCCAG CAACACAGCC AACACTAAGA CTGTTTGTCA CCCTGTTCAA 
ATTGACAGAA CCTGCAATGC AATAACAAGC GAGAAGACTG AACAAGCAAT GACCGAGAAT 
CAAAGCAACA AGTGCAGCAG CGGCGTTGAA TCCTGCATCA TCGACGTGGA TCAAAATCCT 
GCTGAGCAAA CCGAGCACAA GACCAGCGTA GCGTCATCCG TGTTAGAAGT TTGTCAGAGT 
GTCACAGATA GATCGAAGAA AGCCGGAGAC AATCCATTAA TATCACTGAA GCAATACCTT 
ACAGTCAACC TAATAAGGTT ATCAGTGCCA CAGAGTCTAG AAGCAGAAAA GACTCCTGAA 
AACAAGAGAG GAAGCGTGGT GCTAAGAGGA AGGAGCAGGC AGAGTCTTAG ACCAGCGGCC 
ATGACAGCAC AGTCAGAAAG AAGAGTGTCC AGATCTCAGC AGAGGCAGGG AGGAAGTTCA 
GAGATCTGCA CATCTGAAAG CAACAGAGGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104930715 ( XM_027285559.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_027141360.1 Larimichthys crocea histone-lysine N-methyltransferase KMT5B (LOC104930715), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OLa202055
Clone ID Related Accession (Same CDS sequence)	XM_027285559.1
Accession Version	XM_027285559.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	uncharacterized protein LOC104930715 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040022.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGGAT GCACTAGAAT GAGTGTGAAG GAGCTGTGTG AGACCGATGA CCTGGCCACC 
GGTTTGGTGC TGGACCCCCT GCTGGGCTTC AGCACCCACA AAATGAACAT CAGCCCCCCT 
CCTGAGATCC GGCGTTGGGG TAATCTCAAA GAGACCCTGT TGCGCTTTCA GCGTACGCAT 
GACTTCAATG CTACGTTTGA GGCGCTGACG GTGGGCGAGC TGGCTGGTGA TTACTTCAGT 
GCTTTGGGAA GTCACCGTCA GGAGCTGCTG AGGCAACATG TGTATCGCTA CCTCAGTGCC 
TTCCTGTTGG ATAGCGGTAT CAAGATCGAG TCCTGTGACC GATACTCCTC AGAGACCAAT 
GGAGCAAAGA TAACCTCAAC AAGGCACTGG TTTGCAGGAG AGCGGGTGGA GGTCCTGCTG 
GGCTGCATAG CAGAGCTGAG CCCCGCTGAC AGTGCCGTGC TGAGGACGGG AGTCAATGAC 
TTCAGTGTGA TGTATTCCAC GCGCAAACGC TGCGCTCAGC TCTGGCTCGG GCCTGCAGCT 
TTCATTAACC ATGACTGCAA ACCAAACTGC AAGTTCGTCC CTGGTGAGAA GAATATCGCT 
TGTGTTGAAG CCATTCGACC TATCTCACCT GGGGAGGAGA TCACATGTTA CTATGGTGCC 
AGCTTTTTTG GGGAAGGAAA TGAAATGTGT GAATGCTGCA CCTGTGAGAG GAACGGAGAG 
GGCCACTTCA AACACAGAGG GAAGCAGCCT GAATGTGAAG AAACAAAGGA CCCCGTGGGC 
CAAAAGTACC GTCTGAGAGA GCGATATCTT CGTCATCACA GAGAGAAGGG TCACTTTCCT 
ACACGGCAGA TAATACCAGG ATTACACTCA GGTATCTTTA AAGCCATCCC CTCAAGGAAT 
TCCTTTACCC AGCAGATGAG GAGAAATGCC TTGAAGAACA GAAAATTTAG CAAATCAAAG 
AAGTGGAGAC GGGAGCACAG ACGATCAGGA AAGCACCCGC TGACATCCTC CACGGCCAAC 
CAGTGTCGGA CAGTTCCACT TTTATCCCAA GTTACTCTGA GGGACCTCAG AATCAGAGTA 
CGGCGCCACT CAGTGGATTT CTTACTCCAT TGTAAGGATC CTAAAAGCAA AGAAAGGGCT 
CTGCTTCAAC AACTGGAGGA AGTGAAGCCC CTGAGTGAAC TGAATACGAC TGGTGAAGGG 
AATAACAACA GTGAGGTAAA CTTGAAGCCC TTCACTCTAA ATTCCCCCAT GTCTGTTCAT 
CAGAAAAGTA GACCTGCAGG AGGCAGCGCA GGCCTTAATG GAAAGTCTGT TAAAGTGGTG 
GATAGGCAGG TGGCTCTGAG GACATCTTCG AGAACAAGAA GCATGGTTCA CAACACGGTG 
ACACATCAAA CAGTGTCCAG CAACACAGCC AACACTAAGA CTGTTTGTCA CCCTGTTCAA 
ATTGACAGAA CCTGCAATGC AATAACAAGC GAGAAGACTG AACAAGCAAT GACCGAGAAT 
CAAAGCAACA AGTGCAGCAG CGGCGTTGAA TCCTGCATCA TCGACGTGGA TCAAAATCCT 
GCTGAGCAAA CCGAGCACAA GACCAGCGTA GCGTCATCCG TGTTAGAAGT TTGTCAGAGT 
GTCACAGATA GATCGAAGAA AGCCGGAGAC AATCCATTAA TATCACTGAA GCAATACCTT 
ACAGTCAACC TAATAAGGTT ATCAGTGCCA CAGAGTCTAG AAGCAGAAAA GACTCCTGAA 
AACAAGAGAG GAAGCGTGGT GCTAAGAGGA AGGAGCAGGC AGAGTCTTAG ACCAGCGGCC 
ATGACAGCAC AGTCAGAAAG AAGAGTGTCC AGATCTCAGC AGAGGCAGGG AGGAAGTTCA 
GAGATCTGCA CATCTGAAAG CAACAGAGGG GTGAGAGAAA TGTTTTTCAA GGCCATTAAT 
AAAGTAAATT TAAGGAAGCG ACATGCAAGT GAATCTGTTG GTGATTTATC CCACAGTGAG 
AAAGGGGAGA ATGACAAGGT TATCGAAATC CAAACCGTGC CAGAAGAAAA AGCTGATATA 
CAAAAGGAGC AGCAGGACAT CGTAAAAGGC AGACTTGAAG GGCAGAAAGA AAAGAGAAAT 
GCTGCATCTA CAGTTAAGGA ATCTAAAAGT AACAGAGTCC TGGAAGAAGT GCAAGAGCAT 
GAATGTAAAG TAGTGCCCAG TGAAAAACAA AATAACATGC AATGTATTAG AAATATAGTC 
AATGATTTAT GTAAGGAGGA GGGAGTTGTT AAACTATCAG AACAAATGGC TAAGAGTGTG 
AATAAACAAC CAGCATGTAG GCTTGAAGAA GATGTTGTTA TAAAACAGGC AAAGGTTCTG 
CTATCTGATA TTTTAAGAAA GGATAAACAA TTAATAGATA AGCAATTTCT AGGAGGAACT 
GTAGGAAGAG GATTTTCTAC CTTAGCCTCT AAAATAGAGA GCGCTGAGGA GGAAGAAGAA 
CAGACTGAAA TAAGTGGAGG CAGATCCAAT TGTTCAGTGC AGAGGAGGAT TATAAGACGG 
CGAATGATGA GGCCTAGAGC AGCGAAAACA AAAGCAGAAG GTAAGCAAAG CACCAAGGGA 
CTCTTGAACA AAGAAGTTCC AGATAGAGTA AAGACAGAGT TGAGCGAGGA AACTTTGCAG 
CTTCAGTCTC CACAGATGCA TAACGACCCA CCGGCCACCT GCCTCAACCC ACTACCTCAT 
AAGTCACTAA TTTCAGCAAA AGCCACGACC AATCTGCATC CAGACACCAA TCTGCATCCA 
GACACCAATC CACAGGCACA CATTCAGTCC AACATACCAC TGAAGAAGCG TACATTTCGT 
AGTAATGTGG AAGTAGGTTT GAATCCTGCT TCGGATCGGG CTTCTAAAGA AGCTGCAGAG 
CTAAATTCAT CCACAGAGTT GACGCAGGGC CTAACCACAT GTTCAGATGA GAGTAGCAAA 
GTAAAAAGAG ACTGTAAAGG TAGATGTAGG AGAACAGAGG TCCAGAGGTT AACCACCAAA 
AGGATCACAA CATTTAACCG AGTGCTTCGC TCCAGAGCTA ATGAAGTCCA ACGGAGCCAT 
TTATTCAGGA AAGTCCGAAA GTGTAAATTA GAGAGGCTAG ATGAGGATGA CATTAACAAG 
TCTCAGACAC TACAAAGTGA TCATCTTGCA AATGACAAAC ATGCTGATTA TCCTGCTGAA 
CTCCAACAGC ACTGTGGTAA AAATCCAAAT GAATGTCTCA AGGAAGATAG GGTGGAGACG 
TTAATCCAGC AAAGTGCTTC GGAGGATACG AAGCCAGACC TCAATTTTAA GATTCGTTTT 
AAAAGGAGGA GAGGGAGAGT ATGGGAGATG CAAAGAGCAG GATTTGAAAA TCTGGCGTTA 
AAATCAGAAA AAGGAGACGA GATGACATGC GATCCTTTTA AAGCCATCAT GGATTCGGTG 
TCGGTTTTAA ACATGGAGAT GGAGGCAGCT CAGGCTCATG TGCAGGCTAG TAAGAAGTCA 
AAGCTTAGAC TGCATCGATT GAAAAAAAGA GGTCAGAGGC TCCGTGAAAG GCAAACCGTA 
AACGTCCCCT CACACGTAAA ATCAGACCAA GATCTTGATA ATAGCGAAAA AGTATGTGAC 
GCGCCCAGAG ACAAAGCAGA AATTAAAGAG AAGCTCGACG TAGTCAAGGT GCTGCCAGGA 
AAAATTGAGA GTTGTACAGA TGACGGTCAT GTGTTGTCAG CTCAAGGGCT AGAAACAAAA 
ATTGAAGATC GTTTTGTAAA GAACTGCTGC TTGTTTGAAA GTGGGTCACA GCAGAAACTT 
CAGCCAGAAT TGGACTCAAA TGGTCTTCCA TTACCCGTAA TTAAACTACG AAGAAAGACA 
GAGGATATTT GGGAAGTGGA CAGCAAAGAG GCTCTTCGAC ACACTGATGT TAAAGTAGAG 
CCCAATGTTA AGAAAGAGAT GAAGGGTCAC ATTATCGGGA AACAAAAGAA AGAATGTCTT 
GATTTTAGCA AATTTAAGGA AGAGTGTCCG TCTGCACAGA GACTGAACAG CAACTCAGAC 
CTTCTGAAAA CAGAACCACC ACCGTTTTCC TTATCTCTGT CACCACTGTC TCTGTCTTCC 
CCTCTTAGCG ACAATAGAAC TGAAGTTATA ACCACGGCTG CAGATACAGT CATTGAAAGG 
CCAGAGATGA ACAGCGGAGG AAGGAAACAG AGGCATAAAA TGGAGAGAAC ACGCAAGTGT 
GGGCCTGAAG AAACACCCAC CACTTGTCTT AGTCACACTC TCCAACAAAT TGACAACAGT 
CTCTCTCGGC TTTCCGAGGG CTTGTGTTCA TCTCAGACTT TGGAAAAGCC GACCGCCTGT 
TCCAGTGCTT CTAATTCTGT CATCCAGCCC CCGTCCCAGT CTCCTCCATT CACAACTGGA 
GATAACATGC TTTCTGGTGA GCCCAGCTTC ACAAACTGCT GTGATGACAT CCTGGACTTT 
CAGTGTCTCA ACTTTGAGGG GTATTATCAG CCACAGAATA TACTTCCATC TTCCCCATCA 
GATCTTTGTT CATTGGATCC ACCCACAGAT CCTTTCAGCA GTCCTCTCTC TCACAGCCCT 
TCTGATACAT GGACCACTGA AACGCCATAC CTTGGCCCTC CCAGTCCAGG AAATAACTTT 
ACCAGTGAGG ATCTGCAGTT TTTTCCAGGT CTAATTTCTT CCAAAGGTGA CTCTGTTCCT 
CTGGAATGTG ACGCGAGGGA TACCTCCAAA GACAGAAACC TACCAAACCC CAGTTTCAGT 
TTTTCAACAC TTGGCAACTC TGACCTAACT GCTAAGGATC GGATTATAAG TAAAAATCCA 
GGAATGAGGC TCTGCAGAGA AACTCAGCCC ATGTCTGTAG TCAACAAGCC TCGGCTGTTT 
GGTACAACAT CATCTGTTAC ATCTCAAAAT CCTCTTACTT TGACCCAACA GCCCATCAAC 
ATCAAGGCGA GCACTAGTCA ATCAAAAATC CAGTCTAATC TCAGAACCCA GGGCCCCTTC 
CATCGTATGA CCATTCCTAG TAAATCTCAG CCTTTTGCAA GTAGTCAGCC AAATGCAGTC 
AGAGGAGTGT CCCAGAGTTG CTTGCCTAAA TCCGTCCCAT CATCTCAAAT CCCTAACAAA 
TTTGTTTCTC CTCAGCTTTT CACTGTGAGA AATCCCAATC CTCCTGACAA TCCATTCAAA 
GCCCATGAGA AACCATCTTC AGTCATTCAC AGGGTGCTTA AGTTTCAGGG GGGGAACCAG 
AGTCAGAACT TGTATAGTGC ACCCTGCAAA GACACCCTGG CTGCTGGCAG TCCAACTATC 
ACATCCCGAA CACCCATCTC CAGATCTGGT GCTATTGAGA AGCAGAGTCA AAGTCAGAAA 
CTAGGTGTTT GCACAGATGG TCATCACAAA GGCAACATAA CTGTTCAAGG ATTTGCAAAG 
GATGTCGACC ATCTTGGTCC CTCCAGCCAC CCCAGCAGGT CACACCCTCA TTTCAACAAA 
CTCAACTCTT TTGCTCAGTC CTTTAGTAAA AGTAAGATGT CTTCTGAAAA ACATGAAAAT 
GTTGAAACCA ACATGACATA CAAAAACCTT TCAGCGCTAC CAAGACCCTT CTTCTTTCCA 
AGCAAAATGT CTGACGGTTA TTCATCGCTA CAAGACAAGA GTTTAAGACA GGATAAATCC 
TCAACATCTA GCTCAGACAA GCCCCATCCA TGTTACACTC AGCAAGACCC TTTTGATTTT 
AGCTTCGGCT CGTCTCTTTC ACCCATGTCT CAGCACAACA GCCCCCAAGT GATCCACAGT 
ACACCTCCTG GTACGCCAGC ACCAGCAAAC AAGAGTCAGT CCTCAGCCTC TTTCCCTTAT 
GGCTATCAAG GCCCTCCTTA CGTACTAAAC TTCAGTGGAG ATCATTCATT GACCCTGGGT 
CTCAGGGATG GGGCTGATGG TTACCCAGGT TTAGGCTCGG CAAACTACAC CTACCACTGC 
CTGATGGAAC CTTCAGGCAC CCAAGGCCGG CTGGTTCTCG AGCCCTGTGA ACCCCAGTTA 
TCTAACCCAG CATCCTTTTC CTTGGGTGGC TTTTCTGGAC TCAAAGGTCA GGATGAACTT 
TACAGAAAGG ATATGCAGCA ACAGTGCCAG CCTGGGGAGC ACCAAGGCCC ACCACACTAT 
GGACCCGTAC CATCGTCTCA CTCCATGGGT GCTACAAAAC CCAAGAGAGT GAGGTTAGTG 
GTGACAGATG GCACAGTAGA CTTAGATCTA CAGTACTCGG ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027141360.1
CDS	389..6613
Translation	MDGCTRMSVKELCETDDLATGLVLDPLLGFSTHKMNISPPPEIRRWGNLKETLLRFQRTHDFNATFEALTVGELAGDYFSALGSHRQELLRQHVYRYLSAFLLDSGIKIESCDRYSSETNGAKITSTRHWFAGERVEVLLGCIAELSPADSAVLRTGVNDFSVMYSTRKRCAQLWLGPAAFINHDCKPNCKFVPGEKNIACVEAIRPISPGEEITCYYGASFFGEGNEMCECCTCERNGEGHFKHRGKQPECEETKDPVGQKYRLRERYLRHHREKGHFPTRQIIPGLHSGIFKAIPSRNSFTQQMRRNALKNRKFSKSKKWRREHRRSGKHPLTSSTANQCRTVPLLSQVTLRDLRIRVRRHSVDFLLHCKDPKSKERALLQQLEEVKPLSELNTTGEGNNNSEVNLKPFTLNSPMSVHQKSRPAGGSAGLNGKSVKVVDRQVALRTSSRTRSMVHNTVTHQTVSSNTANTKTVCHPVQIDRTCNAITSEKTEQAMTENQSNKCSSGVESCIIDVDQNPAEQTEHKTSVASSVLEVCQSVTDRSKKAGDNPLISLKQYLTVNLIRLSVPQSLEAEKTPENKRGSVVLRGRSRQSLRPAAMTAQSERRVSRSQQRQGGSSEICTSESNRGVREMFFKAINKVNLRKRHASESVGDLSHSEKGENDKVIEIQTVPEEKADIQKEQQDIVKGRLEGQKEKRNAASTVKESKSNRVLEEVQEHECKVVPSEKQNNMQCIRNIVNDLCKEEGVVKLSEQMAKSVNKQPACRLEEDVVIKQAKVLLSDILRKDKQLIDKQFLGGTVGRGFSTLASKIESAEEEEEQTEISGGRSNCSVQRRIIRRRMMRPRAAKTKAEGKQSTKGLLNKEVPDRVKTELSEETLQLQSPQMHNDPPATCLNPLPHKSLISAKATTNLHPDTNLHPDTNPQAHIQSNIPLKKRTFRSNVEVGLNPASDRASKEAAELNSSTELTQGLTTCSDESSKVKRDCKGRCRRTEVQRLTTKRITTFNRVLRSRANEVQRSHLFRKVRKCKLERLDEDDINKSQTLQSDHLANDKHADYPAELQQHCGKNPNECLKEDRVETLIQQSASEDTKPDLNFKIRFKRRRGRVWEMQRAGFENLALKSEKGDEMTCDPFKAIMDSVSVLNMEMEAAQAHVQASKKSKLRLHRLKKRGQRLRERQTVNVPSHVKSDQDLDNSEKVCDAPRDKAEIKEKLDVVKVLPGKIESCTDDGHVLSAQGLETKIEDRFVKNCCLFESGSQQKLQPELDSNGLPLPVIKLRRKTEDIWEVDSKEALRHTDVKVEPNVKKEMKGHIIGKQKKECLDFSKFKEECPSAQRLNSNSDLLKTEPPPFSLSLSPLSLSSPLSDNRTEVITTAADTVIERPEMNSGGRKQRHKMERTRKCGPEETPTTCLSHTLQQIDNSLSRLSEGLCSSQTLEKPTACSSASNSVIQPPSQSPPFTTGDNMLSGEPSFTNCCDDILDFQCLNFEGYYQPQNILPSSPSDLCSLDPPTDPFSSPLSHSPSDTWTTETPYLGPPSPGNNFTSEDLQFFPGLISSKGDSVPLECDARDTSKDRNLPNPSFSFSTLGNSDLTAKDRIISKNPGMRLCRETQPMSVVNKPRLFGTTSSVTSQNPLTLTQQPINIKASTSQSKIQSNLRTQGPFHRMTIPSKSQPFASSQPNAVRGVSQSCLPKSVPSSQIPNKFVSPQLFTVRNPNPPDNPFKAHEKPSSVIHRVLKFQGGNQSQNLYSAPCKDTLAAGSPTITSRTPISRSGAIEKQSQSQKLGVCTDGHHKGNITVQGFAKDVDHLGPSSHPSRSHPHFNKLNSFAQSFSKSKMSSEKHENVETNMTYKNLSALPRPFFFPSKMSDGYSSLQDKSLRQDKSSTSSSDKPHPCYTQQDPFDFSFGSSLSPMSQHNSPQVIHSTPPGTPAPANKSQSSASFPYGYQGPPYVLNFSGDHSLTLGLRDGADGYPGLGSANYTYHCLMEPSGTQGRLVLEPCEPQLSNPASFSLGGFSGLKGQDELYRKDMQQQCQPGEHQGPPHYGPVPSSHSMGATKPKRVRLVVTDGTVDLDLQYSD

Target ORF information:

RefSeq Version	XM_027285559.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea histone-lysine N-methyltransferase KMT5B (LOC104930715), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027285559.1

ORF Insert Sequence:

ATGGACGGAT GCACTAGAAT GAGTGTGAAG GAGCTGTGTG AGACCGATGA CCTGGCCACC 
GGTTTGGTGC TGGACCCCCT GCTGGGCTTC AGCACCCACA AAATGAACAT CAGCCCCCCT 
CCTGAGATCC GGCGTTGGGG TAATCTCAAA GAGACCCTGT TGCGCTTTCA GCGTACGCAT 
GACTTCAATG CTACGTTTGA GGCGCTGACG GTGGGCGAGC TGGCTGGTGA TTACTTCAGT 
GCTTTGGGAA GTCACCGTCA GGAGCTGCTG AGGCAACATG TGTATCGCTA CCTCAGTGCC 
TTCCTGTTGG ATAGCGGTAT CAAGATCGAG TCCTGTGACC GATACTCCTC AGAGACCAAT 
GGAGCAAAGA TAACCTCAAC AAGGCACTGG TTTGCAGGAG AGCGGGTGGA GGTCCTGCTG 
GGCTGCATAG CAGAGCTGAG CCCCGCTGAC AGTGCCGTGC TGAGGACGGG AGTCAATGAC 
TTCAGTGTGA TGTATTCCAC GCGCAAACGC TGCGCTCAGC TCTGGCTCGG GCCTGCAGCT 
TTCATTAACC ATGACTGCAA ACCAAACTGC AAGTTCGTCC CTGGTGAGAA GAATATCGCT 
TGTGTTGAAG CCATTCGACC TATCTCACCT GGGGAGGAGA TCACATGTTA CTATGGTGCC 
AGCTTTTTTG GGGAAGGAAA TGAAATGTGT GAATGCTGCA CCTGTGAGAG GAACGGAGAG 
GGCCACTTCA AACACAGAGG GAAGCAGCCT GAATGTGAAG AAACAAAGGA CCCCGTGGGC 
CAAAAGTACC GTCTGAGAGA GCGATATCTT CGTCATCACA GAGAGAAGGG TCACTTTCCT 
ACACGGCAGA TAATACCAGG ATTACACTCA GGTATCTTTA AAGCCATCCC CTCAAGGAAT 
TCCTTTACCC AGCAGATGAG GAGAAATGCC TTGAAGAACA GAAAATTTAG CAAATCAAAG 
AAGTGGAGAC GGGAGCACAG ACGATCAGGA AAGCACCCGC TGACATCCTC CACGGCCAAC 
CAGTGTCGGA CAGTTCCACT TTTATCCCAA GTTACTCTGA GGGACCTCAG AATCAGAGTA 
CGGCGCCACT CAGTGGATTT CTTACTCCAT TGTAAGGATC CTAAAAGCAA AGAAAGGGCT 
CTGCTTCAAC AACTGGAGGA AGTGAAGCCC CTGAGTGAAC TGAATACGAC TGGTGAAGGG 
AATAACAACA GTGAGGTAAA CTTGAAGCCC TTCACTCTAA ATTCCCCCAT GTCTGTTCAT 
CAGAAAAGTA GACCTGCAGG AGGCAGCGCA GGCCTTAATG GAAAGTCTGT TAAAGTGGTG 
GATAGGCAGG TGGCTCTGAG GACATCTTCG AGAACAAGAA GCATGGTTCA CAACACGGTG 
ACACATCAAA CAGTGTCCAG CAACACAGCC AACACTAAGA CTGTTTGTCA CCCTGTTCAA 
ATTGACAGAA CCTGCAATGC AATAACAAGC GAGAAGACTG AACAAGCAAT GACCGAGAAT 
CAAAGCAACA AGTGCAGCAG CGGCGTTGAA TCCTGCATCA TCGACGTGGA TCAAAATCCT 
GCTGAGCAAA CCGAGCACAA GACCAGCGTA GCGTCATCCG TGTTAGAAGT TTGTCAGAGT 
GTCACAGATA GATCGAAGAA AGCCGGAGAC AATCCATTAA TATCACTGAA GCAATACCTT 
ACAGTCAACC TAATAAGGTT ATCAGTGCCA CAGAGTCTAG AAGCAGAAAA GACTCCTGAA 
AACAAGAGAG GAAGCGTGGT GCTAAGAGGA AGGAGCAGGC AGAGTCTTAG ACCAGCGGCC 
ATGACAGCAC AGTCAGAAAG AAGAGTGTCC AGATCTCAGC AGAGGCAGGG AGGAAGTTCA 
GAGATCTGCA CATCTGAAAG CAACAGAGGG GTGAGAGAAA TGTTTTTCAA GGCCATTAAT 
AAAGTAAATT TAAGGAAGCG ACATGCAAGT GAATCTGTTG GTGATTTATC CCACAGTGAG 
AAAGGGGAGA ATGACAAGGT TATCGAAATC CAAACCGTGC CAGAAGAAAA AGCTGATATA 
CAAAAGGAGC AGCAGGACAT CGTAAAAGGC AGACTTGAAG GGCAGAAAGA AAAGAGAAAT 
GCTGCATCTA CAGTTAAGGA ATCTAAAAGT AACAGAGTCC TGGAAGAAGT GCAAGAGCAT 
GAATGTAAAG TAGTGCCCAG TGAAAAACAA AATAACATGC AATGTATTAG AAATATAGTC 
AATGATTTAT GTAAGGAGGA GGGAGTTGTT AAACTATCAG AACAAATGGC TAAGAGTGTG 
AATAAACAAC CAGCATGTAG GCTTGAAGAA GATGTTGTTA TAAAACAGGC AAAGGTTCTG 
CTATCTGATA TTTTAAGAAA GGATAAACAA TTAATAGATA AGCAATTTCT AGGAGGAACT 
GTAGGAAGAG GATTTTCTAC CTTAGCCTCT AAAATAGAGA GCGCTGAGGA GGAAGAAGAA 
CAGACTGAAA TAAGTGGAGG CAGATCCAAT TGTTCAGTGC AGAGGAGGAT TATAAGACGG 
CGAATGATGA GGCCTAGAGC AGCGAAAACA AAAGCAGAAG GTAAGCAAAG CACCAAGGGA 
CTCTTGAACA AAGAAGTTCC AGATAGAGTA AAGACAGAGT TGAGCGAGGA AACTTTGCAG 
CTTCAGTCTC CACAGATGCA TAACGACCCA CCGGCCACCT GCCTCAACCC ACTACCTCAT 
AAGTCACTAA TTTCAGCAAA AGCCACGACC AATCTGCATC CAGACACCAA TCTGCATCCA 
GACACCAATC CACAGGCACA CATTCAGTCC AACATACCAC TGAAGAAGCG TACATTTCGT 
AGTAATGTGG AAGTAGGTTT GAATCCTGCT TCGGATCGGG CTTCTAAAGA AGCTGCAGAG 
CTAAATTCAT CCACAGAGTT GACGCAGGGC CTAACCACAT GTTCAGATGA GAGTAGCAAA 
GTAAAAAGAG ACTGTAAAGG TAGATGTAGG AGAACAGAGG TCCAGAGGTT AACCACCAAA 
AGGATCACAA CATTTAACCG AGTGCTTCGC TCCAGAGCTA ATGAAGTCCA ACGGAGCCAT 
TTATTCAGGA AAGTCCGAAA GTGTAAATTA GAGAGGCTAG ATGAGGATGA CATTAACAAG 
TCTCAGACAC TACAAAGTGA TCATCTTGCA AATGACAAAC ATGCTGATTA TCCTGCTGAA 
CTCCAACAGC ACTGTGGTAA AAATCCAAAT GAATGTCTCA AGGAAGATAG GGTGGAGACG 
TTAATCCAGC AAAGTGCTTC GGAGGATACG AAGCCAGACC TCAATTTTAA GATTCGTTTT 
AAAAGGAGGA GAGGGAGAGT ATGGGAGATG CAAAGAGCAG GATTTGAAAA TCTGGCGTTA 
AAATCAGAAA AAGGAGACGA GATGACATGC GATCCTTTTA AAGCCATCAT GGATTCGGTG 
TCGGTTTTAA ACATGGAGAT GGAGGCAGCT CAGGCTCATG TGCAGGCTAG TAAGAAGTCA 
AAGCTTAGAC TGCATCGATT GAAAAAAAGA GGTCAGAGGC TCCGTGAAAG GCAAACCGTA 
AACGTCCCCT CACACGTAAA ATCAGACCAA GATCTTGATA ATAGCGAAAA AGTATGTGAC 
GCGCCCAGAG ACAAAGCAGA AATTAAAGAG AAGCTCGACG TAGTCAAGGT GCTGCCAGGA 
AAAATTGAGA GTTGTACAGA TGACGGTCAT GTGTTGTCAG CTCAAGGGCT AGAAACAAAA 
ATTGAAGATC GTTTTGTAAA GAACTGCTGC TTGTTTGAAA GTGGGTCACA GCAGAAACTT 
CAGCCAGAAT TGGACTCAAA TGGTCTTCCA TTACCCGTAA TTAAACTACG AAGAAAGACA 
GAGGATATTT GGGAAGTGGA CAGCAAAGAG GCTCTTCGAC ACACTGATGT TAAAGTAGAG 
CCCAATGTTA AGAAAGAGAT GAAGGGTCAC ATTATCGGGA AACAAAAGAA AGAATGTCTT 
GATTTTAGCA AATTTAAGGA AGAGTGTCCG TCTGCACAGA GACTGAACAG CAACTCAGAC 
CTTCTGAAAA CAGAACCACC ACCGTTTTCC TTATCTCTGT CACCACTGTC TCTGTCTTCC 
CCTCTTAGCG ACAATAGAAC TGAAGTTATA ACCACGGCTG CAGATACAGT CATTGAAAGG 
CCAGAGATGA ACAGCGGAGG AAGGAAACAG AGGCATAAAA TGGAGAGAAC ACGCAAGTGT 
GGGCCTGAAG AAACACCCAC CACTTGTCTT AGTCACACTC TCCAACAAAT TGACAACAGT 
CTCTCTCGGC TTTCCGAGGG CTTGTGTTCA TCTCAGACTT TGGAAAAGCC GACCGCCTGT 
TCCAGTGCTT CTAATTCTGT CATCCAGCCC CCGTCCCAGT CTCCTCCATT CACAACTGGA 
GATAACATGC TTTCTGGTGA GCCCAGCTTC ACAAACTGCT GTGATGACAT CCTGGACTTT 
CAGTGTCTCA ACTTTGAGGG GTATTATCAG CCACAGAATA TACTTCCATC TTCCCCATCA 
GATCTTTGTT CATTGGATCC ACCCACAGAT CCTTTCAGCA GTCCTCTCTC TCACAGCCCT 
TCTGATACAT GGACCACTGA AACGCCATAC CTTGGCCCTC CCAGTCCAGG AAATAACTTT 
ACCAGTGAGG ATCTGCAGTT TTTTCCAGGT CTAATTTCTT CCAAAGGTGA CTCTGTTCCT 
CTGGAATGTG ACGCGAGGGA TACCTCCAAA GACAGAAACC TACCAAACCC CAGTTTCAGT 
TTTTCAACAC TTGGCAACTC TGACCTAACT GCTAAGGATC GGATTATAAG TAAAAATCCA 
GGAATGAGGC TCTGCAGAGA AACTCAGCCC ATGTCTGTAG TCAACAAGCC TCGGCTGTTT 
GGTACAACAT CATCTGTTAC ATCTCAAAAT CCTCTTACTT TGACCCAACA GCCCATCAAC 
ATCAAGGCGA GCACTAGTCA ATCAAAAATC CAGTCTAATC TCAGAACCCA GGGCCCCTTC 
CATCGTATGA CCATTCCTAG TAAATCTCAG CCTTTTGCAA GTAGTCAGCC AAATGCAGTC 
AGAGGAGTGT CCCAGAGTTG CTTGCCTAAA TCCGTCCCAT CATCTCAAAT CCCTAACAAA 
TTTGTTTCTC CTCAGCTTTT CACTGTGAGA AATCCCAATC CTCCTGACAA TCCATTCAAA 
GCCCATGAGA AACCATCTTC AGTCATTCAC AGGGTGCTTA AGTTTCAGGG GGGGAACCAG 
AGTCAGAACT TGTATAGTGC ACCCTGCAAA GACACCCTGG CTGCTGGCAG TCCAACTATC 
ACATCCCGAA CACCCATCTC CAGATCTGGT GCTATTGAGA AGCAGAGTCA AAGTCAGAAA 
CTAGGTGTTT GCACAGATGG TCATCACAAA GGCAACATAA CTGTTCAAGG ATTTGCAAAG 
GATGTCGACC ATCTTGGTCC CTCCAGCCAC CCCAGCAGGT CACACCCTCA TTTCAACAAA 
CTCAACTCTT TTGCTCAGTC CTTTAGTAAA AGTAAGATGT CTTCTGAAAA ACATGAAAAT 
GTTGAAACCA ACATGACATA CAAAAACCTT TCAGCGCTAC CAAGACCCTT CTTCTTTCCA 
AGCAAAATGT CTGACGGTTA TTCATCGCTA CAAGACAAGA GTTTAAGACA GGATAAATCC 
TCAACATCTA GCTCAGACAA GCCCCATCCA TGTTACACTC AGCAAGACCC TTTTGATTTT 
AGCTTCGGCT CGTCTCTTTC ACCCATGTCT CAGCACAACA GCCCCCAAGT GATCCACAGT 
ACACCTCCTG GTACGCCAGC ACCAGCAAAC AAGAGTCAGT CCTCAGCCTC TTTCCCTTAT 
GGCTATCAAG GCCCTCCTTA CGTACTAAAC TTCAGTGGAG ATCATTCATT GACCCTGGGT 
CTCAGGGATG GGGCTGATGG TTACCCAGGT TTAGGCTCGG CAAACTACAC CTACCACTGC 
CTGATGGAAC CTTCAGGCAC CCAAGGCCGG CTGGTTCTCG AGCCCTGTGA ACCCCAGTTA 
TCTAACCCAG CATCCTTTTC CTTGGGTGGC TTTTCTGGAC TCAAAGGTCA GGATGAACTT 
TACAGAAAGG ATATGCAGCA ACAGTGCCAG CCTGGGGAGC ACCAAGGCCC ACCACACTAT 
GGACCCGTAC CATCGTCTCA CTCCATGGGT GCTACAAAAC CCAAGAGAGT GAGGTTAGTG 
GTGACAGATG GCACAGTAGA CTTAGATCTA CAGTACTCGG ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104930715 ( XM_027285560.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_027141361.1 Larimichthys crocea histone-lysine N-methyltransferase KMT5B (LOC104930715), transcript variant X2, mRNA.

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CloneID	OLa202056
Clone ID Related Accession (Same CDS sequence)	XM_027285560.1
Accession Version	XM_027285560.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	uncharacterized protein LOC104930715 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040022.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGGAT GCACTAGAAT GAGTGTGAAG GAGCTGTGTG AGACCGATGA CCTGGCCACC 
GGTTTGGTGC TGGACCCCCT GCTGGGCTTC AGCACCCACA AAATGAACAT CAGCCCCCCT 
CCTGAGATCC GGCGTTGGGG TAATCTCAAA GAGACCCTGT TGCGCTTTCA GCGTACGCAT 
GACTTCAATG CTACGTTTGA GGCGCTGACG GTGGGCGAGC TGGCTGGTGA TTACTTCAGT 
GCTTTGGGAA GTCACCGTCA GGAGCTGCTG AGGCAACATG TGTATCGCTA CCTCAGTGCC 
TTCCTGTTGG ATAGCGGTAT CAAGATCGAG TCCTGTGACC GATACTCCTC AGAGACCAAT 
GGAGCAAAGA TAACCTCAAC AAGGCACTGG TTTGCAGGAG AGCGGGTGGA GGTCCTGCTG 
GGCTGCATAG CAGAGCTGAG CCCCGCTGAC AGTGCCGTGC TGAGGACGGG AGTCAATGAC 
TTCAGTGTGA TGTATTCCAC GCGCAAACGC TGCGCTCAGC TCTGGCTCGG GCCTGCAGCT 
TTCATTAACC ATGACTGCAA ACCAAACTGC AAGTTCGTCC CTGGTGAGAA GAATATCGCT 
TGTGTTGAAG CCATTCGACC TATCTCACCT GGGGAGGAGA TCACATGTTA CTATGGTGCC 
AGCTTTTTTG GGGAAGGAAA TGAAATGTGT GAATGCTGCA CCTGTGAGAG GAACGGAGAG 
GGCCACTTCA AACACAGAGG GAAGCAGCCT GAATGTGAAG AAACAAAGGA CCCCGTGGGC 
CAAAAGTACC GTCTGAGAGA GCGATATCTT CGTCATCACA GAGAGAAGGG TCACTTTCCT 
ACACGGCAGA TAATACCAGG ATTACACTCA GCCATCCCCT CAAGGAATTC CTTTACCCAG 
CAGATGAGGA GAAATGCCTT GAAGAACAGA AAATTTAGCA AATCAAAGAA GTGGAGACGG 
GAGCACAGAC GATCAGGAAA GCACCCGCTG ACATCCTCCA CGGCCAACCA GTGTCGGACA 
GTTCCACTTT TATCCCAAGT TACTCTGAGG GACCTCAGAA TCAGAGTACG GCGCCACTCA 
GTGGATTTCT TACTCCATTG TAAGGATCCT AAAAGCAAAG AAAGGGCTCT GCTTCAACAA 
CTGGAGGAAG TGAAGCCCCT GAGTGAACTG AATACGACTG GTGAAGGGAA TAACAACAGT 
GAGGTAAACT TGAAGCCCTT CACTCTAAAT TCCCCCATGT CTGTTCATCA GAAAAGTAGA 
CCTGCAGGAG GCAGCGCAGG CCTTAATGGA AAGTCTGTTA AAGTGGTGGA TAGGCAGGTG 
GCTCTGAGGA CATCTTCGAG AACAAGAAGC ATGGTTCACA ACACGGTGAC ACATCAAACA 
GTGTCCAGCA ACACAGCCAA CACTAAGACT GTTTGTCACC CTGTTCAAAT TGACAGAACC 
TGCAATGCAA TAACAAGCGA GAAGACTGAA CAAGCAATGA CCGAGAATCA AAGCAACAAG 
TGCAGCAGCG GCGTTGAATC CTGCATCATC GACGTGGATC AAAATCCTGC TGAGCAAACC 
GAGCACAAGA CCAGCGTAGC GTCATCCGTG TTAGAAGTTT GTCAGAGTGT CACAGATAGA 
TCGAAGAAAG CCGGAGACAA TCCATTAATA TCACTGAAGC AATACCTTAC AGTCAACCTA 
ATAAGGTTAT CAGTGCCACA GAGTCTAGAA GCAGAAAAGA CTCCTGAAAA CAAGAGAGGA 
AGCGTGGTGC TAAGAGGAAG GAGCAGGCAG AGTCTTAGAC CAGCGGCCAT GACAGCACAG 
TCAGAAAGAA GAGTGTCCAG ATCTCAGCAG AGGCAGGGAG GAAGTTCAGA GATCTGCACA 
TCTGAAAGCA ACAGAGGGGT GAGAGAAATG TTTTTCAAGG CCATTAATAA AGTAAATTTA 
AGGAAGCGAC ATGCAAGTGA ATCTGTTGGT GATTTATCCC ACAGTGAGAA AGGGGAGAAT 
GACAAGGTTA TCGAAATCCA AACCGTGCCA GAAGAAAAAG CTGATATACA AAAGGAGCAG 
CAGGACATCG TAAAAGGCAG ACTTGAAGGG CAGAAAGAAA AGAGAAATGC TGCATCTACA 
GTTAAGGAAT CTAAAAGTAA CAGAGTCCTG GAAGAAGTGC AAGAGCATGA ATGTAAAGTA 
GTGCCCAGTG AAAAACAAAA TAACATGCAA TGTATTAGAA ATATAGTCAA TGATTTATGT 
AAGGAGGAGG GAGTTGTTAA ACTATCAGAA CAAATGGCTA AGAGTGTGAA TAAACAACCA 
GCATGTAGGC TTGAAGAAGA TGTTGTTATA AAACAGGCAA AGGTTCTGCT ATCTGATATT 
TTAAGAAAGG ATAAACAATT AATAGATAAG CAATTTCTAG GAGGAACTGT AGGAAGAGGA 
TTTTCTACCT TAGCCTCTAA AATAGAGAGC GCTGAGGAGG AAGAAGAACA GACTGAAATA 
AGTGGAGGCA GATCCAATTG TTCAGTGCAG AGGAGGATTA TAAGACGGCG AATGATGAGG 
CCTAGAGCAG CGAAAACAAA AGCAGAAGGT AAGCAAAGCA CCAAGGGACT CTTGAACAAA 
GAAGTTCCAG ATAGAGTAAA GACAGAGTTG AGCGAGGAAA CTTTGCAGCT TCAGTCTCCA 
CAGATGCATA ACGACCCACC GGCCACCTGC CTCAACCCAC TACCTCATAA GTCACTAATT 
TCAGCAAAAG CCACGACCAA TCTGCATCCA GACACCAATC TGCATCCAGA CACCAATCCA 
CAGGCACACA TTCAGTCCAA CATACCACTG AAGAAGCGTA CATTTCGTAG TAATGTGGAA 
GTAGGTTTGA ATCCTGCTTC GGATCGGGCT TCTAAAGAAG CTGCAGAGCT AAATTCATCC 
ACAGAGTTGA CGCAGGGCCT AACCACATGT TCAGATGAGA GTAGCAAAGT AAAAAGAGAC 
TGTAAAGGTA GATGTAGGAG AACAGAGGTC CAGAGGTTAA CCACCAAAAG GATCACAACA 
TTTAACCGAG TGCTTCGCTC CAGAGCTAAT GAAGTCCAAC GGAGCCATTT ATTCAGGAAA 
GTCCGAAAGT GTAAATTAGA GAGGCTAGAT GAGGATGACA TTAACAAGTC TCAGACACTA 
CAAAGTGATC ATCTTGCAAA TGACAAACAT GCTGATTATC CTGCTGAACT CCAACAGCAC 
TGTGGTAAAA ATCCAAATGA ATGTCTCAAG GAAGATAGGG TGGAGACGTT AATCCAGCAA 
AGTGCTTCGG AGGATACGAA GCCAGACCTC AATTTTAAGA TTCGTTTTAA AAGGAGGAGA 
GGGAGAGTAT GGGAGATGCA AAGAGCAGGA TTTGAAAATC TGGCGTTAAA ATCAGAAAAA 
GGAGACGAGA TGACATGCGA TCCTTTTAAA GCCATCATGG ATTCGGTGTC GGTTTTAAAC 
ATGGAGATGG AGGCAGCTCA GGCTCATGTG CAGGCTAGTA AGAAGTCAAA GCTTAGACTG 
CATCGATTGA AAAAAAGAGG TCAGAGGCTC CGTGAAAGGC AAACCGTAAA CGTCCCCTCA 
CACGTAAAAT CAGACCAAGA TCTTGATAAT AGCGAAAAAG TATGTGACGC GCCCAGAGAC 
AAAGCAGAAA TTAAAGAGAA GCTCGACGTA GTCAAGGTGC TGCCAGGAAA AATTGAGAGT 
TGTACAGATG ACGGTCATGT GTTGTCAGCT CAAGGGCTAG AAACAAAAAT TGAAGATCGT 
TTTGTAAAGA ACTGCTGCTT GTTTGAAAGT GGGTCACAGC AGAAACTTCA GCCAGAATTG 
GACTCAAATG GTCTTCCATT ACCCGTAATT AAACTACGAA GAAAGACAGA GGATATTTGG 
GAAGTGGACA GCAAAGAGGC TCTTCGACAC ACTGATGTTA AAGTAGAGCC CAATGTTAAG 
AAAGAGATGA AGGGTCACAT TATCGGGAAA CAAAAGAAAG AATGTCTTGA TTTTAGCAAA 
TTTAAGGAAG AGTGTCCGTC TGCACAGAGA CTGAACAGCA ACTCAGACCT TCTGAAAACA 
GAACCACCAC CGTTTTCCTT ATCTCTGTCA CCACTGTCTC TGTCTTCCCC TCTTAGCGAC 
AATAGAACTG AAGTTATAAC CACGGCTGCA GATACAGTCA TTGAAAGGCC AGAGATGAAC 
AGCGGAGGAA GGAAACAGAG GCATAAAATG GAGAGAACAC GCAAGTGTGG GCCTGAAGAA 
ACACCCACCA CTTGTCTTAG TCACACTCTC CAACAAATTG ACAACAGTCT CTCTCGGCTT 
TCCGAGGGCT TGTGTTCATC TCAGACTTTG GAAAAGCCGA CCGCCTGTTC CAGTGCTTCT 
AATTCTGTCA TCCAGCCCCC GTCCCAGTCT CCTCCATTCA CAACTGGAGA TAACATGCTT 
TCTGGTGAGC CCAGCTTCAC AAACTGCTGT GATGACATCC TGGACTTTCA GTGTCTCAAC 
TTTGAGGGGT ATTATCAGCC ACAGAATATA CTTCCATCTT CCCCATCAGA TCTTTGTTCA 
TTGGATCCAC CCACAGATCC TTTCAGCAGT CCTCTCTCTC ACAGCCCTTC TGATACATGG 
ACCACTGAAA CGCCATACCT TGGCCCTCCC AGTCCAGGAA ATAACTTTAC CAGTGAGGAT 
CTGCAGTTTT TTCCAGGTCT AATTTCTTCC AAAGGTGACT CTGTTCCTCT GGAATGTGAC 
GCGAGGGATA CCTCCAAAGA CAGAAACCTA CCAAACCCCA GTTTCAGTTT TTCAACACTT 
GGCAACTCTG ACCTAACTGC TAAGGATCGG ATTATAAGTA AAAATCCAGG AATGAGGCTC 
TGCAGAGAAA CTCAGCCCAT GTCTGTAGTC AACAAGCCTC GGCTGTTTGG TACAACATCA 
TCTGTTACAT CTCAAAATCC TCTTACTTTG ACCCAACAGC CCATCAACAT CAAGGCGAGC 
ACTAGTCAAT CAAAAATCCA GTCTAATCTC AGAACCCAGG GCCCCTTCCA TCGTATGACC 
ATTCCTAGTA AATCTCAGCC TTTTGCAAGT AGTCAGCCAA ATGCAGTCAG AGGAGTGTCC 
CAGAGTTGCT TGCCTAAATC CGTCCCATCA TCTCAAATCC CTAACAAATT TGTTTCTCCT 
CAGCTTTTCA CTGTGAGAAA TCCCAATCCT CCTGACAATC CATTCAAAGC CCATGAGAAA 
CCATCTTCAG TCATTCACAG GGTGCTTAAG TTTCAGGGGG GGAACCAGAG TCAGAACTTG 
TATAGTGCAC CCTGCAAAGA CACCCTGGCT GCTGGCAGTC CAACTATCAC ATCCCGAACA 
CCCATCTCCA GATCTGGTGC TATTGAGAAG CAGAGTCAAA GTCAGAAACT AGGTGTTTGC 
ACAGATGGTC ATCACAAAGG CAACATAACT GTTCAAGGAT TTGCAAAGGA TGTCGACCAT 
CTTGGTCCCT CCAGCCACCC CAGCAGGTCA CACCCTCATT TCAACAAACT CAACTCTTTT 
GCTCAGTCCT TTAGTAAAAG TAAGATGTCT TCTGAAAAAC ATGAAAATGT TGAAACCAAC 
ATGACATACA AAAACCTTTC AGCGCTACCA AGACCCTTCT TCTTTCCAAG CAAAATGTCT 
GACGGTTATT CATCGCTACA AGACAAGAGT TTAAGACAGG ATAAATCCTC AACATCTAGC 
TCAGACAAGC CCCATCCATG TTACACTCAG CAAGACCCTT TTGATTTTAG CTTCGGCTCG 
TCTCTTTCAC CCATGTCTCA GCACAACAGC CCCCAAGTGA TCCACAGTAC ACCTCCTGGT 
ACGCCAGCAC CAGCAAACAA GAGTCAGTCC TCAGCCTCTT TCCCTTATGG CTATCAAGGC 
CCTCCTTACG TACTAAACTT CAGTGGAGAT CATTCATTGA CCCTGGGTCT CAGGGATGGG 
GCTGATGGTT ACCCAGGTTT AGGCTCGGCA AACTACACCT ACCACTGCCT GATGGAACCT 
TCAGGCACCC AAGGCCGGCT GGTTCTCGAG CCCTGTGAAC CCCAGTTATC TAACCCAGCA 
TCCTTTTCCT TGGGTGGCTT TTCTGGACTC AAAGGTCAGG ATGAACTTTA CAGAAAGGAT 
ATGCAGCAAC AGTGCCAGCC TGGGGAGCAC CAAGGCCCAC CACACTATGG ACCCGTACCA 
TCGTCTCACT CCATGGGTGC TACAAAACCC AAGAGAGTGA GGTTAGTGGT GACAGATGGC 
ACAGTAGACT TAGATCTACA GTACTCGGAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027141361.1
CDS	390..6602
Translation	MDGCTRMSVKELCETDDLATGLVLDPLLGFSTHKMNISPPPEIRRWGNLKETLLRFQRTHDFNATFEALTVGELAGDYFSALGSHRQELLRQHVYRYLSAFLLDSGIKIESCDRYSSETNGAKITSTRHWFAGERVEVLLGCIAELSPADSAVLRTGVNDFSVMYSTRKRCAQLWLGPAAFINHDCKPNCKFVPGEKNIACVEAIRPISPGEEITCYYGASFFGEGNEMCECCTCERNGEGHFKHRGKQPECEETKDPVGQKYRLRERYLRHHREKGHFPTRQIIPGLHSAIPSRNSFTQQMRRNALKNRKFSKSKKWRREHRRSGKHPLTSSTANQCRTVPLLSQVTLRDLRIRVRRHSVDFLLHCKDPKSKERALLQQLEEVKPLSELNTTGEGNNNSEVNLKPFTLNSPMSVHQKSRPAGGSAGLNGKSVKVVDRQVALRTSSRTRSMVHNTVTHQTVSSNTANTKTVCHPVQIDRTCNAITSEKTEQAMTENQSNKCSSGVESCIIDVDQNPAEQTEHKTSVASSVLEVCQSVTDRSKKAGDNPLISLKQYLTVNLIRLSVPQSLEAEKTPENKRGSVVLRGRSRQSLRPAAMTAQSERRVSRSQQRQGGSSEICTSESNRGVREMFFKAINKVNLRKRHASESVGDLSHSEKGENDKVIEIQTVPEEKADIQKEQQDIVKGRLEGQKEKRNAASTVKESKSNRVLEEVQEHECKVVPSEKQNNMQCIRNIVNDLCKEEGVVKLSEQMAKSVNKQPACRLEEDVVIKQAKVLLSDILRKDKQLIDKQFLGGTVGRGFSTLASKIESAEEEEEQTEISGGRSNCSVQRRIIRRRMMRPRAAKTKAEGKQSTKGLLNKEVPDRVKTELSEETLQLQSPQMHNDPPATCLNPLPHKSLISAKATTNLHPDTNLHPDTNPQAHIQSNIPLKKRTFRSNVEVGLNPASDRASKEAAELNSSTELTQGLTTCSDESSKVKRDCKGRCRRTEVQRLTTKRITTFNRVLRSRANEVQRSHLFRKVRKCKLERLDEDDINKSQTLQSDHLANDKHADYPAELQQHCGKNPNECLKEDRVETLIQQSASEDTKPDLNFKIRFKRRRGRVWEMQRAGFENLALKSEKGDEMTCDPFKAIMDSVSVLNMEMEAAQAHVQASKKSKLRLHRLKKRGQRLRERQTVNVPSHVKSDQDLDNSEKVCDAPRDKAEIKEKLDVVKVLPGKIESCTDDGHVLSAQGLETKIEDRFVKNCCLFESGSQQKLQPELDSNGLPLPVIKLRRKTEDIWEVDSKEALRHTDVKVEPNVKKEMKGHIIGKQKKECLDFSKFKEECPSAQRLNSNSDLLKTEPPPFSLSLSPLSLSSPLSDNRTEVITTAADTVIERPEMNSGGRKQRHKMERTRKCGPEETPTTCLSHTLQQIDNSLSRLSEGLCSSQTLEKPTACSSASNSVIQPPSQSPPFTTGDNMLSGEPSFTNCCDDILDFQCLNFEGYYQPQNILPSSPSDLCSLDPPTDPFSSPLSHSPSDTWTTETPYLGPPSPGNNFTSEDLQFFPGLISSKGDSVPLECDARDTSKDRNLPNPSFSFSTLGNSDLTAKDRIISKNPGMRLCRETQPMSVVNKPRLFGTTSSVTSQNPLTLTQQPINIKASTSQSKIQSNLRTQGPFHRMTIPSKSQPFASSQPNAVRGVSQSCLPKSVPSSQIPNKFVSPQLFTVRNPNPPDNPFKAHEKPSSVIHRVLKFQGGNQSQNLYSAPCKDTLAAGSPTITSRTPISRSGAIEKQSQSQKLGVCTDGHHKGNITVQGFAKDVDHLGPSSHPSRSHPHFNKLNSFAQSFSKSKMSSEKHENVETNMTYKNLSALPRPFFFPSKMSDGYSSLQDKSLRQDKSSTSSSDKPHPCYTQQDPFDFSFGSSLSPMSQHNSPQVIHSTPPGTPAPANKSQSSASFPYGYQGPPYVLNFSGDHSLTLGLRDGADGYPGLGSANYTYHCLMEPSGTQGRLVLEPCEPQLSNPASFSLGGFSGLKGQDELYRKDMQQQCQPGEHQGPPHYGPVPSSHSMGATKPKRVRLVVTDGTVDLDLQYSD

Target ORF information:

RefSeq Version	XM_027285560.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea histone-lysine N-methyltransferase KMT5B (LOC104930715), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027285560.1

ORF Insert Sequence:

ATGGACGGAT GCACTAGAAT GAGTGTGAAG GAGCTGTGTG AGACCGATGA CCTGGCCACC 
GGTTTGGTGC TGGACCCCCT GCTGGGCTTC AGCACCCACA AAATGAACAT CAGCCCCCCT 
CCTGAGATCC GGCGTTGGGG TAATCTCAAA GAGACCCTGT TGCGCTTTCA GCGTACGCAT 
GACTTCAATG CTACGTTTGA GGCGCTGACG GTGGGCGAGC TGGCTGGTGA TTACTTCAGT 
GCTTTGGGAA GTCACCGTCA GGAGCTGCTG AGGCAACATG TGTATCGCTA CCTCAGTGCC 
TTCCTGTTGG ATAGCGGTAT CAAGATCGAG TCCTGTGACC GATACTCCTC AGAGACCAAT 
GGAGCAAAGA TAACCTCAAC AAGGCACTGG TTTGCAGGAG AGCGGGTGGA GGTCCTGCTG 
GGCTGCATAG CAGAGCTGAG CCCCGCTGAC AGTGCCGTGC TGAGGACGGG AGTCAATGAC 
TTCAGTGTGA TGTATTCCAC GCGCAAACGC TGCGCTCAGC TCTGGCTCGG GCCTGCAGCT 
TTCATTAACC ATGACTGCAA ACCAAACTGC AAGTTCGTCC CTGGTGAGAA GAATATCGCT 
TGTGTTGAAG CCATTCGACC TATCTCACCT GGGGAGGAGA TCACATGTTA CTATGGTGCC 
AGCTTTTTTG GGGAAGGAAA TGAAATGTGT GAATGCTGCA CCTGTGAGAG GAACGGAGAG 
GGCCACTTCA AACACAGAGG GAAGCAGCCT GAATGTGAAG AAACAAAGGA CCCCGTGGGC 
CAAAAGTACC GTCTGAGAGA GCGATATCTT CGTCATCACA GAGAGAAGGG TCACTTTCCT 
ACACGGCAGA TAATACCAGG ATTACACTCA GCCATCCCCT CAAGGAATTC CTTTACCCAG 
CAGATGAGGA GAAATGCCTT GAAGAACAGA AAATTTAGCA AATCAAAGAA GTGGAGACGG 
GAGCACAGAC GATCAGGAAA GCACCCGCTG ACATCCTCCA CGGCCAACCA GTGTCGGACA 
GTTCCACTTT TATCCCAAGT TACTCTGAGG GACCTCAGAA TCAGAGTACG GCGCCACTCA 
GTGGATTTCT TACTCCATTG TAAGGATCCT AAAAGCAAAG AAAGGGCTCT GCTTCAACAA 
CTGGAGGAAG TGAAGCCCCT GAGTGAACTG AATACGACTG GTGAAGGGAA TAACAACAGT 
GAGGTAAACT TGAAGCCCTT CACTCTAAAT TCCCCCATGT CTGTTCATCA GAAAAGTAGA 
CCTGCAGGAG GCAGCGCAGG CCTTAATGGA AAGTCTGTTA AAGTGGTGGA TAGGCAGGTG 
GCTCTGAGGA CATCTTCGAG AACAAGAAGC ATGGTTCACA ACACGGTGAC ACATCAAACA 
GTGTCCAGCA ACACAGCCAA CACTAAGACT GTTTGTCACC CTGTTCAAAT TGACAGAACC 
TGCAATGCAA TAACAAGCGA GAAGACTGAA CAAGCAATGA CCGAGAATCA AAGCAACAAG 
TGCAGCAGCG GCGTTGAATC CTGCATCATC GACGTGGATC AAAATCCTGC TGAGCAAACC 
GAGCACAAGA CCAGCGTAGC GTCATCCGTG TTAGAAGTTT GTCAGAGTGT CACAGATAGA 
TCGAAGAAAG CCGGAGACAA TCCATTAATA TCACTGAAGC AATACCTTAC AGTCAACCTA 
ATAAGGTTAT CAGTGCCACA GAGTCTAGAA GCAGAAAAGA CTCCTGAAAA CAAGAGAGGA 
AGCGTGGTGC TAAGAGGAAG GAGCAGGCAG AGTCTTAGAC CAGCGGCCAT GACAGCACAG 
TCAGAAAGAA GAGTGTCCAG ATCTCAGCAG AGGCAGGGAG GAAGTTCAGA GATCTGCACA 
TCTGAAAGCA ACAGAGGGGT GAGAGAAATG TTTTTCAAGG CCATTAATAA AGTAAATTTA 
AGGAAGCGAC ATGCAAGTGA ATCTGTTGGT GATTTATCCC ACAGTGAGAA AGGGGAGAAT 
GACAAGGTTA TCGAAATCCA AACCGTGCCA GAAGAAAAAG CTGATATACA AAAGGAGCAG 
CAGGACATCG TAAAAGGCAG ACTTGAAGGG CAGAAAGAAA AGAGAAATGC TGCATCTACA 
GTTAAGGAAT CTAAAAGTAA CAGAGTCCTG GAAGAAGTGC AAGAGCATGA ATGTAAAGTA 
GTGCCCAGTG AAAAACAAAA TAACATGCAA TGTATTAGAA ATATAGTCAA TGATTTATGT 
AAGGAGGAGG GAGTTGTTAA ACTATCAGAA CAAATGGCTA AGAGTGTGAA TAAACAACCA 
GCATGTAGGC TTGAAGAAGA TGTTGTTATA AAACAGGCAA AGGTTCTGCT ATCTGATATT 
TTAAGAAAGG ATAAACAATT AATAGATAAG CAATTTCTAG GAGGAACTGT AGGAAGAGGA 
TTTTCTACCT TAGCCTCTAA AATAGAGAGC GCTGAGGAGG AAGAAGAACA GACTGAAATA 
AGTGGAGGCA GATCCAATTG TTCAGTGCAG AGGAGGATTA TAAGACGGCG AATGATGAGG 
CCTAGAGCAG CGAAAACAAA AGCAGAAGGT AAGCAAAGCA CCAAGGGACT CTTGAACAAA 
GAAGTTCCAG ATAGAGTAAA GACAGAGTTG AGCGAGGAAA CTTTGCAGCT TCAGTCTCCA 
CAGATGCATA ACGACCCACC GGCCACCTGC CTCAACCCAC TACCTCATAA GTCACTAATT 
TCAGCAAAAG CCACGACCAA TCTGCATCCA GACACCAATC TGCATCCAGA CACCAATCCA 
CAGGCACACA TTCAGTCCAA CATACCACTG AAGAAGCGTA CATTTCGTAG TAATGTGGAA 
GTAGGTTTGA ATCCTGCTTC GGATCGGGCT TCTAAAGAAG CTGCAGAGCT AAATTCATCC 
ACAGAGTTGA CGCAGGGCCT AACCACATGT TCAGATGAGA GTAGCAAAGT AAAAAGAGAC 
TGTAAAGGTA GATGTAGGAG AACAGAGGTC CAGAGGTTAA CCACCAAAAG GATCACAACA 
TTTAACCGAG TGCTTCGCTC CAGAGCTAAT GAAGTCCAAC GGAGCCATTT ATTCAGGAAA 
GTCCGAAAGT GTAAATTAGA GAGGCTAGAT GAGGATGACA TTAACAAGTC TCAGACACTA 
CAAAGTGATC ATCTTGCAAA TGACAAACAT GCTGATTATC CTGCTGAACT CCAACAGCAC 
TGTGGTAAAA ATCCAAATGA ATGTCTCAAG GAAGATAGGG TGGAGACGTT AATCCAGCAA 
AGTGCTTCGG AGGATACGAA GCCAGACCTC AATTTTAAGA TTCGTTTTAA AAGGAGGAGA 
GGGAGAGTAT GGGAGATGCA AAGAGCAGGA TTTGAAAATC TGGCGTTAAA ATCAGAAAAA 
GGAGACGAGA TGACATGCGA TCCTTTTAAA GCCATCATGG ATTCGGTGTC GGTTTTAAAC 
ATGGAGATGG AGGCAGCTCA GGCTCATGTG CAGGCTAGTA AGAAGTCAAA GCTTAGACTG 
CATCGATTGA AAAAAAGAGG TCAGAGGCTC CGTGAAAGGC AAACCGTAAA CGTCCCCTCA 
CACGTAAAAT CAGACCAAGA TCTTGATAAT AGCGAAAAAG TATGTGACGC GCCCAGAGAC 
AAAGCAGAAA TTAAAGAGAA GCTCGACGTA GTCAAGGTGC TGCCAGGAAA AATTGAGAGT 
TGTACAGATG ACGGTCATGT GTTGTCAGCT CAAGGGCTAG AAACAAAAAT TGAAGATCGT 
TTTGTAAAGA ACTGCTGCTT GTTTGAAAGT GGGTCACAGC AGAAACTTCA GCCAGAATTG 
GACTCAAATG GTCTTCCATT ACCCGTAATT AAACTACGAA GAAAGACAGA GGATATTTGG 
GAAGTGGACA GCAAAGAGGC TCTTCGACAC ACTGATGTTA AAGTAGAGCC CAATGTTAAG 
AAAGAGATGA AGGGTCACAT TATCGGGAAA CAAAAGAAAG AATGTCTTGA TTTTAGCAAA 
TTTAAGGAAG AGTGTCCGTC TGCACAGAGA CTGAACAGCA ACTCAGACCT TCTGAAAACA 
GAACCACCAC CGTTTTCCTT ATCTCTGTCA CCACTGTCTC TGTCTTCCCC TCTTAGCGAC 
AATAGAACTG AAGTTATAAC CACGGCTGCA GATACAGTCA TTGAAAGGCC AGAGATGAAC 
AGCGGAGGAA GGAAACAGAG GCATAAAATG GAGAGAACAC GCAAGTGTGG GCCTGAAGAA 
ACACCCACCA CTTGTCTTAG TCACACTCTC CAACAAATTG ACAACAGTCT CTCTCGGCTT 
TCCGAGGGCT TGTGTTCATC TCAGACTTTG GAAAAGCCGA CCGCCTGTTC CAGTGCTTCT 
AATTCTGTCA TCCAGCCCCC GTCCCAGTCT CCTCCATTCA CAACTGGAGA TAACATGCTT 
TCTGGTGAGC CCAGCTTCAC AAACTGCTGT GATGACATCC TGGACTTTCA GTGTCTCAAC 
TTTGAGGGGT ATTATCAGCC ACAGAATATA CTTCCATCTT CCCCATCAGA TCTTTGTTCA 
TTGGATCCAC CCACAGATCC TTTCAGCAGT CCTCTCTCTC ACAGCCCTTC TGATACATGG 
ACCACTGAAA CGCCATACCT TGGCCCTCCC AGTCCAGGAA ATAACTTTAC CAGTGAGGAT 
CTGCAGTTTT TTCCAGGTCT AATTTCTTCC AAAGGTGACT CTGTTCCTCT GGAATGTGAC 
GCGAGGGATA CCTCCAAAGA CAGAAACCTA CCAAACCCCA GTTTCAGTTT TTCAACACTT 
GGCAACTCTG ACCTAACTGC TAAGGATCGG ATTATAAGTA AAAATCCAGG AATGAGGCTC 
TGCAGAGAAA CTCAGCCCAT GTCTGTAGTC AACAAGCCTC GGCTGTTTGG TACAACATCA 
TCTGTTACAT CTCAAAATCC TCTTACTTTG ACCCAACAGC CCATCAACAT CAAGGCGAGC 
ACTAGTCAAT CAAAAATCCA GTCTAATCTC AGAACCCAGG GCCCCTTCCA TCGTATGACC 
ATTCCTAGTA AATCTCAGCC TTTTGCAAGT AGTCAGCCAA ATGCAGTCAG AGGAGTGTCC 
CAGAGTTGCT TGCCTAAATC CGTCCCATCA TCTCAAATCC CTAACAAATT TGTTTCTCCT 
CAGCTTTTCA CTGTGAGAAA TCCCAATCCT CCTGACAATC CATTCAAAGC CCATGAGAAA 
CCATCTTCAG TCATTCACAG GGTGCTTAAG TTTCAGGGGG GGAACCAGAG TCAGAACTTG 
TATAGTGCAC CCTGCAAAGA CACCCTGGCT GCTGGCAGTC CAACTATCAC ATCCCGAACA 
CCCATCTCCA GATCTGGTGC TATTGAGAAG CAGAGTCAAA GTCAGAAACT AGGTGTTTGC 
ACAGATGGTC ATCACAAAGG CAACATAACT GTTCAAGGAT TTGCAAAGGA TGTCGACCAT 
CTTGGTCCCT CCAGCCACCC CAGCAGGTCA CACCCTCATT TCAACAAACT CAACTCTTTT 
GCTCAGTCCT TTAGTAAAAG TAAGATGTCT TCTGAAAAAC ATGAAAATGT TGAAACCAAC 
ATGACATACA AAAACCTTTC AGCGCTACCA AGACCCTTCT TCTTTCCAAG CAAAATGTCT 
GACGGTTATT CATCGCTACA AGACAAGAGT TTAAGACAGG ATAAATCCTC AACATCTAGC 
TCAGACAAGC CCCATCCATG TTACACTCAG CAAGACCCTT TTGATTTTAG CTTCGGCTCG 
TCTCTTTCAC CCATGTCTCA GCACAACAGC CCCCAAGTGA TCCACAGTAC ACCTCCTGGT 
ACGCCAGCAC CAGCAAACAA GAGTCAGTCC TCAGCCTCTT TCCCTTATGG CTATCAAGGC 
CCTCCTTACG TACTAAACTT CAGTGGAGAT CATTCATTGA CCCTGGGTCT CAGGGATGGG 
GCTGATGGTT ACCCAGGTTT AGGCTCGGCA AACTACACCT ACCACTGCCT GATGGAACCT 
TCAGGCACCC AAGGCCGGCT GGTTCTCGAG CCCTGTGAAC CCCAGTTATC TAACCCAGCA 
TCCTTTTCCT TGGGTGGCTT TTCTGGACTC AAAGGTCAGG ATGAACTTTA CAGAAAGGAT 
ATGCAGCAAC AGTGCCAGCC TGGGGAGCAC CAAGGCCCAC CACACTATGG ACCCGTACCA 
TCGTCTCACT CCATGGGTGC TACAAAACCC AAGAGAGTGA GGTTAGTGGT GACAGATGGC 
ACAGTAGACT TAGATCTACA GTACTCGGAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104930715 ( XM_019256099.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019111644.1 Larimichthys crocea histone-lysine N-methyltransferase KMT5B-like (LOC104930715), transcript variant X1, mRNA.

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CloneID	OLa86930
Clone ID Related Accession (Same CDS sequence)	XM_019256099.1
Accession Version	XM_019256099.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	uncharacterized protein LOC104930715 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017608059.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGGAT GCACTAGAAT GAGTGTGAAG GAGCTGTGTG AGACCGATGA CCTGGCCACC 
GGTTTGGTGC TGGACCCCCT GCTGGGCTTC AGCACCCACA AAATGAACAT CAGCCCCCCT 
CCTGAGATCC GGCGTTGGGG TAATCTCAAA GAGACCCTGT TGCGCTTTCA GCGTACGCAT 
GACTTCAATG CTACGTTTGA GGCGCTGACG GTGGGCGAGC TGGCTGGTGA TTACTTCAGT 
GCTTTGGGAA GTCACCGTCA GGAGCTGCTG AGGCAACATG TGTATCGCTA CCTCAGTGCC 
TTCCTGTTGG ATAGCGGTAT CAAGATAGAG TCCTGTGACC GATACTCCTC AGAGACCAAT 
GGAGCAAAGA TAACCTCAAC AAGGCACTGG TTTGCAGGAG AGCGGGTGGA GGTCCTGCTG 
GGCTGCATAG CAGAGCTGAG CCCCGCTGAC AGTGCCGTGC TGAGGACGGG AGTCAATGAC 
TTCAGTGTGA TGTATTCCAC GCGCAAACGC TGCGCTCAGC TCTGGCTCGG GCCTGCAGCT 
TTCATTAACC ATGACTGCAA ACCAAACTGC AAGTTCGTCC CTGGTGAGAA GAATATCGCT 
TGTGTTGAAG CCATTCGACC TATCTCACCT GGGGAGGAGA TCACATGTTA CTATGGTGCC 
AGCTTTTTTG GGGAAGGAAA TGAAATGTGT GAATGCTGCA CCTGTGAGAG GAACGGAGAG 
GGCCACTTCA AACACAGAGG GAAGCAGCCT GAATGTGAAG AAACAAAGGA CCCCGTGGGC 
CAAAAGTACC GTCTGAGAGA GCGATATCTT CGTCATCACA GAGAGAAGGG TCACTTTCCT 
ACACGGCAGA TAATACCAGG ATTACACTCA GGTATCTTTA AAGCCATCCC CTCAAGGAAT 
TCCTTTACCC AGCAGATGAG GAGAAATGCC TTGAAGAACA GAAAATTTAG CAAATCAAAG 
AAGTGGAGAC GGGAGCACAG ACGATCAGGA AAGCACCCGC TGACATCCTC CACGGCCAAC 
CAGTGTCGGA CAGTTCCACT TTTATCCCAA GTTACTCTGA GGGACCTCAG AATCAGAGTA 
CGGCGCCACT CAGTGGATTT CTTACTCCAT TGTAAGGATC CTAAAAGCAA AGAAAGGGCT 
CTGCTTCAAC AACTGGAGGA AGTGAAGCCC CTGAGTGAAC TGAATACGAC TGGTGAAGGG 
AATAACAACA GTGAGGTAAA CTTGAAGCCC TTCACTCTAA ATTCCCCCAT GCCTGTTCAT 
CAGAAAAGTA GACATGCAGG AGGCAGCGCA GGCCTTAATG GAAAGTCTGT TAAAGTGGTG 
GATAGGCAGG TGGCTCTGAG GACATCTTCG AGAACAAGAA GCATGGTTCA CAACACGGTG 
ACACATCAAA CAGTGTCCAG CAACACAGCC AACACTAAGA CTGTTTGTCA CCCTGTTCAA 
ATTGACAGAA CCTGCAATGC AATAACAAGC GAGAAGACTG AACAAGCAAT GACCGAGAAT 
CAAAGCAACA AGTGCAGCAG CGGCGTTGAA TCCTGCATCA TCGACGTGGA TCAAAATCCT 
GCTGAGCAAA CCGAGCACAA GACCAGCGTA GCGTCATCCG TGTTAGAAGT TTGTCAGAGT 
GTCACAGATA GATCGAAGAA AGCCGGAGAC AATCCATTAA TATCACTGAA GCAATACCTT 
ACAGTCAACC TAATAAGGTT ATCAGTGCCA CAGAGTCTAG AAGCAGAAAA GACTCCTGAA 
AACAAGAGAG GAAGCGTGGT GCTAAGAGGA AGGAGCAGGC AGAGTCTTAG ACCAGCGGCC 
ATGACAGCAC AGTCAGAAAG AAGAGTGTCC AGATCTCAGC AGAGGCAGGG AGGAAGTTCA 
GAGATCTGCA CATCTGAAAG CAACAGAGGG GTGAGAGAAA TGTTTTTCAA GGCCATTAAT 
AAAGTAAATT TAAGGAAGCG ACATGCAAGT GAATCTGTTG GTGATTTATC CCACAGTGAG 
AAAGGGGAGA ATGACAAGGT TATCGAAATC CAAACCGTGC CAGAAGAAAA AGCTGATATA 
CAAAAGGAGC AGCAGGACAT CGTAAAAGGC AGACTTGAAG GGCAGAAAGA AAAGAGAAAT 
GCTGCATCTA CAGTTAAGGA ATCTAAAAGT AACAGAGTCC TGGAAGAAGT GCAAGAGCAT 
GAATGTAAAG TAGTGCCCAG TGAAAAACAA AATAACATGC AATGTATTAG AAATATAGTC 
AATGATTTAT GTAAGGAGGA GGGAGTTGTT AAACTATCAG AACAAATGGC TAAGAGTGTG 
AATAAACAAC CAGCATGTAG GCTTGAAGAA GATGTTGTTA TAAAACAGGC AAAGGTTCTG 
CTATCTGATA TTTTAAGAAA GGATAAACAA TTAATAGATA AGCAATTTCT AGGAGGAACT 
GTAGGAAGAG GATTTTCTAC CTTAGCCTCT AAAATAGAGA GCGCTGAGGA GGAAGAAGAA 
CAGACTGAAA TAAGTGGAGG CAGATCCAAT TGTTCAGTGC AGAGGAGGAT TATAAGACGG 
CGAATGATGA GGCCTAGAGC AGCGAAAACA AAAGCAGAAG GTAAGCAAAG CACCAAGGGA 
CTCTTGAACA AAGAAGTTCC AGATAGAGTA AAGACAGAGT TGAGCGAGGA AACTTTGCAG 
CTTCAGTCTC CACAGATGCA TAACGACCCA CCGGCCACCT GCCTCAACCC ACTACCTCAT 
AAGTCACTAA TTTCAGCAAA AGCCACGACC AATCTGCATC CAGACACCAA TCTGCATCCA 
GACACCAATC CACAGGCACA CATTCAGTCC AACATACCAC TGAAGAAGCG TACATTTCGT 
AGTAATGTGG AAGTAGGTTT GAATCCTGCT TCGGATCGGG CTTCTAAAGA AGCTGCAGAG 
CTAAATTCAT CCACAGAGTT GACGCAGGGC CTAACCACAT GTTCAGATGA GAGTAGCAAA 
GTAAAAAGAG ACTGTAAAGG TAGATGTAGG AGAACAGAGG TCCAGAGGTT AACCACCAAA 
AGGATCACAA CATTTAACCG AGTGCTTCGC TCCAGAGCTA ATGAAGTCCA ACGGAGCCAT 
TTATTCAGGA AAGTCCGAAA GTGTAAATTA GAGAGGCTAG ATGAGGATGA CATTAACAAG 
TCTCAGACAC TACAAAGTGA TCATCTTGCA AATGACAAAC ATGCTGATTA TCCTGCTGAA 
CTCCAACAGC ACTGTGGTAA AAATCCAAAT GAATGTCTCA AGGAAGATAG GGTGGAGACG 
TTAATCCAGC AAAGTGCTTC GGAGGATACG AAGCCAGACC TCAATTTTAA GATTCGTTTT 
AAAAGGAGGA GAGGGAGAGT ATGGGAGATG CAAAGAGCAG GATTTGAAAA TCTGGCGTTA 
AAATCAGAAA AAGGAGACGA GATGACATGC GATCCTTTTA AAGCCATCAT GGATTCGGTG 
TCGGTTTTAA ACATGGAGAT GGAGGCAGCT CAGGCTCATG TGCAGGCTAG TAAGAAGTCA 
AAGCTTAGAC TGCATCGATT GAAAAAAAGA GGTCAGAGGC TCCGTGAAAG GCAAACCGTA 
AACGTCCCCT CACACGTAAA ATCAGACCAA GATCTTGATA ATAGCGAAAA AGTATGTGAC 
GCGCCCAGAG ACAAAGCAGA AATTAAAGAG AAGCTCGACG TAGTCAAGGT GCTGCCAGGA 
AAAATTGAGA GTTGTACAGA TGACGGTCAT GTGTTGTCAG CTCAAGGGCT AGAAACAAAA 
ATTGAAGATC GTTTTGTAAA GAACTGCTGC TTGTTTGAAA GTGGGTCACA GCAGAAACTT 
CAGCCAGAAT TGGACTCAAA TGGTCTTCCA TTACCCGTAA TTAAACTACG AAGAAAGACA 
GAGGATATTT GGGAAGTGGA CAGCAAAGAG GCTCTTCGAC ACACTGATGT TAAAGTAGAG 
CCCAATGTTA AGAAAGAGAT GAAGGGTCAC ATTATCGGGA AACAAAAGAA AGAATGTCTT 
GATTTTAGCA AATTTAAGGA AGAGTGTCCG TCTGCACAGA GACTGAACAG CAACTCAGAC 
CTTCTGAAAA CAGAACCACC ACCGTTTTCC TTATCTCTGT CACCACTGTC TCTGTCTTCC 
CCTCTTAGCG ACAATAGAAC TGAAGTTATA ACCACGGCTG CAGATACAGT CATTGAAAGG 
CCAGAGATGA ACAGCGGAGG AAGGAAACAG AGGCATAAAA TGGAGAGAAC ACGCAAGTGT 
GGGCCTGAAG AAACACCCAC CACTTGTCTT AGTCACACTC TCCAACAAAT TGACAACAGT 
CTCTCTCGGC TTTCCGAGGG CTTGTGTTCA TCTCAGACTT TGGAAAAGCC GACCGCCTGT 
TCCAGTGCTT CTAATTCTGT CATCCAGCCC CCGTCCCAGT CTCCTCCATT CACAACTGGA 
GATAACATGC TTTCTGGTGA GCCCAGCTTC ACAAACTGCT GTGATGACAT CCTGGACTTT 
CAGTGTCTCA ACTTTGAGGG GTATTATCAG CCACAGAATA TACTTCCATC TTCCCCATCA 
GATCTTTGTT CATTGGATCC ACCCACAGAT CCTTTCAGCA GTCCTCTCTC TCACAGCCCT 
TCTGATACAT GGACCACTGA AACGCCATAC CTTGGCCCTC CCAGTCCAGG AAATAACTTT 
ACCAGTGAGG ATCTGCAGTT TTTTCCAGGT CTAATTTCTT CCAAAGGTGA CTCTGTTCCT 
CTGGAATGTG ACGCGAGGGA TACCTCCAAA GACAGAAACC TACCAAACCC CAGTTTCAGT 
TTTTCAACAC TTGGCAACTC TGACCTAACT GCTAAGGATC GGATTATAAG TAAAAATCCA 
GGAATGAGGC TCTGCAGAGA AACTCAGCCC ATGTCTGTAG TCAACAAGCC TCGGCTGTTT 
GGTACAACAT CATCTGTTAC ATCTCAAAAT CCTCTTACTT TGACCCAACA GCCCATCAAC 
ATCAAGGCGA GCACTAGTCA ATCAAAAATC CAGTCTAATC TCAGAACCCA GGGCCCCTTC 
CATCGTATGA CCATTCCTAG TAAATCTCAG CCTTTTGCAA GTAGTCAGCC AAATGCAGTC 
AGAGGAGTGT CCCAGAGTTG CTTGCCTAAA TCCGTCCCAT CATCTCAAAT CCCTAACAAA 
TTTGTTTCTC CTCAGCTTTT CACTGTGAGA AATCCCAATC CTCCTGACAA TCCATTCAAA 
GCCCATGAGA AACCATCTTC AGTCATTCAC AGGGTGCTTA AGTTTCAGGG GGGGAACCAG 
AGTCAGAACT TGTATAGTGC ACCCTGCAAA GACACCCTGG CTGCTGGCAG TCCAACTATC 
ACATCCCGAA CACCCATCTC CAGATCTGGT GCTATTGAGA AGCAGAGTCA AAGTCAGAAA 
CTAGGTGTTT GCACAGATGG TCATCACAAA GGCAACATAA CTGTTCAAGG ATTTGCAAAG 
GATGTCGACC ATCTTGGTCC CTCCAGCCAC CCCAGCAGGT CACACCCTCA TTTCAACAAA 
CTCAACTCTT TTGCTCAGTC CTTTAGTAAA AGTAAGATGT CTTCTGAAAA ACATGAAAAT 
GTTGAAACCA ACATGACATA CAAAAACCTT TCAGCGCTAC CAAGACCCTT CTTCTTTCCA 
AGCAAAATGT CTGACGGTTA TTCATCGCTA CAAGACAAGA GTTTAAGACA GGATAAATCC 
TCAACATCTA GCTCAGACAA GCCCCATCCA TGTTACACTC AGCAAGACCC TTTTGATTTT 
AGCTTCGGCT CGTCTCTTTC ACCCATGTCT CAGCACAACA GCCCCCAAGT GATCCACAGT 
ACACCTCCTG GTACGCCAGC ACCAGCAAAC AAGAGTCAGT CCTCAGCCTC TTTCCCTTAT 
GGCTATCAAG GCCCTCCTTA CGTACTAAAC TTCAGTGGAG ATCATTCATT GACCCTGGGT 
CTCAGGGATG GGGCTGATGG TTACCCAGGT TTAGGCTCGG CAAACTACAC CTACCACTGC 
CTGATGGAAC CTTCAGGCAC CCAAGGCCGG CTGGTTCTCG AGCCCTGTGA ACCCCAGTTA 
TCTAACCCAG CATCCTTTTC CTTGGGTGGC TTTTCTGGAC TCAAAGGTCA GGATGAACTT 
TACAGAAAGG ATATGCAGCA ACAGTGCCAG CCTGGGGAGC ACCAAGGCCC ACCACACTAT 
GGACCCGTAC CATCGTCTCA CTCCATGGGT GCTACAAAAC CCAAGAGAGT GAGGTTAGTG 
GTGACAGATG GCACAGTAGA CTTAGATCTA CAGTACTCGG ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019111644.1
CDS	406..6630
Translation	MDGCTRMSVKELCETDDLATGLVLDPLLGFSTHKMNISPPPEIRRWGNLKETLLRFQRTHDFNATFEALTVGELAGDYFSALGSHRQELLRQHVYRYLSAFLLDSGIKIESCDRYSSETNGAKITSTRHWFAGERVEVLLGCIAELSPADSAVLRTGVNDFSVMYSTRKRCAQLWLGPAAFINHDCKPNCKFVPGEKNIACVEAIRPISPGEEITCYYGASFFGEGNEMCECCTCERNGEGHFKHRGKQPECEETKDPVGQKYRLRERYLRHHREKGHFPTRQIIPGLHSGIFKAIPSRNSFTQQMRRNALKNRKFSKSKKWRREHRRSGKHPLTSSTANQCRTVPLLSQVTLRDLRIRVRRHSVDFLLHCKDPKSKERALLQQLEEVKPLSELNTTGEGNNNSEVNLKPFTLNSPMPVHQKSRHAGGSAGLNGKSVKVVDRQVALRTSSRTRSMVHNTVTHQTVSSNTANTKTVCHPVQIDRTCNAITSEKTEQAMTENQSNKCSSGVESCIIDVDQNPAEQTEHKTSVASSVLEVCQSVTDRSKKAGDNPLISLKQYLTVNLIRLSVPQSLEAEKTPENKRGSVVLRGRSRQSLRPAAMTAQSERRVSRSQQRQGGSSEICTSESNRGVREMFFKAINKVNLRKRHASESVGDLSHSEKGENDKVIEIQTVPEEKADIQKEQQDIVKGRLEGQKEKRNAASTVKESKSNRVLEEVQEHECKVVPSEKQNNMQCIRNIVNDLCKEEGVVKLSEQMAKSVNKQPACRLEEDVVIKQAKVLLSDILRKDKQLIDKQFLGGTVGRGFSTLASKIESAEEEEEQTEISGGRSNCSVQRRIIRRRMMRPRAAKTKAEGKQSTKGLLNKEVPDRVKTELSEETLQLQSPQMHNDPPATCLNPLPHKSLISAKATTNLHPDTNLHPDTNPQAHIQSNIPLKKRTFRSNVEVGLNPASDRASKEAAELNSSTELTQGLTTCSDESSKVKRDCKGRCRRTEVQRLTTKRITTFNRVLRSRANEVQRSHLFRKVRKCKLERLDEDDINKSQTLQSDHLANDKHADYPAELQQHCGKNPNECLKEDRVETLIQQSASEDTKPDLNFKIRFKRRRGRVWEMQRAGFENLALKSEKGDEMTCDPFKAIMDSVSVLNMEMEAAQAHVQASKKSKLRLHRLKKRGQRLRERQTVNVPSHVKSDQDLDNSEKVCDAPRDKAEIKEKLDVVKVLPGKIESCTDDGHVLSAQGLETKIEDRFVKNCCLFESGSQQKLQPELDSNGLPLPVIKLRRKTEDIWEVDSKEALRHTDVKVEPNVKKEMKGHIIGKQKKECLDFSKFKEECPSAQRLNSNSDLLKTEPPPFSLSLSPLSLSSPLSDNRTEVITTAADTVIERPEMNSGGRKQRHKMERTRKCGPEETPTTCLSHTLQQIDNSLSRLSEGLCSSQTLEKPTACSSASNSVIQPPSQSPPFTTGDNMLSGEPSFTNCCDDILDFQCLNFEGYYQPQNILPSSPSDLCSLDPPTDPFSSPLSHSPSDTWTTETPYLGPPSPGNNFTSEDLQFFPGLISSKGDSVPLECDARDTSKDRNLPNPSFSFSTLGNSDLTAKDRIISKNPGMRLCRETQPMSVVNKPRLFGTTSSVTSQNPLTLTQQPINIKASTSQSKIQSNLRTQGPFHRMTIPSKSQPFASSQPNAVRGVSQSCLPKSVPSSQIPNKFVSPQLFTVRNPNPPDNPFKAHEKPSSVIHRVLKFQGGNQSQNLYSAPCKDTLAAGSPTITSRTPISRSGAIEKQSQSQKLGVCTDGHHKGNITVQGFAKDVDHLGPSSHPSRSHPHFNKLNSFAQSFSKSKMSSEKHENVETNMTYKNLSALPRPFFFPSKMSDGYSSLQDKSLRQDKSSTSSSDKPHPCYTQQDPFDFSFGSSLSPMSQHNSPQVIHSTPPGTPAPANKSQSSASFPYGYQGPPYVLNFSGDHSLTLGLRDGADGYPGLGSANYTYHCLMEPSGTQGRLVLEPCEPQLSNPASFSLGGFSGLKGQDELYRKDMQQQCQPGEHQGPPHYGPVPSSHSMGATKPKRVRLVVTDGTVDLDLQYSD

Target ORF information:

RefSeq Version	XM_019256099.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea histone-lysine N-methyltransferase KMT5B-like (LOC104930715), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019256099.1

ORF Insert Sequence:

ATGGACGGAT GCACTAGAAT GAGTGTGAAG GAGCTGTGTG AGACCGATGA CCTGGCCACC 
GGTTTGGTGC TGGACCCCCT GCTGGGCTTC AGCACCCACA AAATGAACAT CAGCCCCCCT 
CCTGAGATCC GGCGTTGGGG TAATCTCAAA GAGACCCTGT TGCGCTTTCA GCGTACGCAT 
GACTTCAATG CTACGTTTGA GGCGCTGACG GTGGGCGAGC TGGCTGGTGA TTACTTCAGT 
GCTTTGGGAA GTCACCGTCA GGAGCTGCTG AGGCAACATG TGTATCGCTA CCTCAGTGCC 
TTCCTGTTGG ATAGCGGTAT CAAGATAGAG TCCTGTGACC GATACTCCTC AGAGACCAAT 
GGAGCAAAGA TAACCTCAAC AAGGCACTGG TTTGCAGGAG AGCGGGTGGA GGTCCTGCTG 
GGCTGCATAG CAGAGCTGAG CCCCGCTGAC AGTGCCGTGC TGAGGACGGG AGTCAATGAC 
TTCAGTGTGA TGTATTCCAC GCGCAAACGC TGCGCTCAGC TCTGGCTCGG GCCTGCAGCT 
TTCATTAACC ATGACTGCAA ACCAAACTGC AAGTTCGTCC CTGGTGAGAA GAATATCGCT 
TGTGTTGAAG CCATTCGACC TATCTCACCT GGGGAGGAGA TCACATGTTA CTATGGTGCC 
AGCTTTTTTG GGGAAGGAAA TGAAATGTGT GAATGCTGCA CCTGTGAGAG GAACGGAGAG 
GGCCACTTCA AACACAGAGG GAAGCAGCCT GAATGTGAAG AAACAAAGGA CCCCGTGGGC 
CAAAAGTACC GTCTGAGAGA GCGATATCTT CGTCATCACA GAGAGAAGGG TCACTTTCCT 
ACACGGCAGA TAATACCAGG ATTACACTCA GGTATCTTTA AAGCCATCCC CTCAAGGAAT 
TCCTTTACCC AGCAGATGAG GAGAAATGCC TTGAAGAACA GAAAATTTAG CAAATCAAAG 
AAGTGGAGAC GGGAGCACAG ACGATCAGGA AAGCACCCGC TGACATCCTC CACGGCCAAC 
CAGTGTCGGA CAGTTCCACT TTTATCCCAA GTTACTCTGA GGGACCTCAG AATCAGAGTA 
CGGCGCCACT CAGTGGATTT CTTACTCCAT TGTAAGGATC CTAAAAGCAA AGAAAGGGCT 
CTGCTTCAAC AACTGGAGGA AGTGAAGCCC CTGAGTGAAC TGAATACGAC TGGTGAAGGG 
AATAACAACA GTGAGGTAAA CTTGAAGCCC TTCACTCTAA ATTCCCCCAT GCCTGTTCAT 
CAGAAAAGTA GACATGCAGG AGGCAGCGCA GGCCTTAATG GAAAGTCTGT TAAAGTGGTG 
GATAGGCAGG TGGCTCTGAG GACATCTTCG AGAACAAGAA GCATGGTTCA CAACACGGTG 
ACACATCAAA CAGTGTCCAG CAACACAGCC AACACTAAGA CTGTTTGTCA CCCTGTTCAA 
ATTGACAGAA CCTGCAATGC AATAACAAGC GAGAAGACTG AACAAGCAAT GACCGAGAAT 
CAAAGCAACA AGTGCAGCAG CGGCGTTGAA TCCTGCATCA TCGACGTGGA TCAAAATCCT 
GCTGAGCAAA CCGAGCACAA GACCAGCGTA GCGTCATCCG TGTTAGAAGT TTGTCAGAGT 
GTCACAGATA GATCGAAGAA AGCCGGAGAC AATCCATTAA TATCACTGAA GCAATACCTT 
ACAGTCAACC TAATAAGGTT ATCAGTGCCA CAGAGTCTAG AAGCAGAAAA GACTCCTGAA 
AACAAGAGAG GAAGCGTGGT GCTAAGAGGA AGGAGCAGGC AGAGTCTTAG ACCAGCGGCC 
ATGACAGCAC AGTCAGAAAG AAGAGTGTCC AGATCTCAGC AGAGGCAGGG AGGAAGTTCA 
GAGATCTGCA CATCTGAAAG CAACAGAGGG GTGAGAGAAA TGTTTTTCAA GGCCATTAAT 
AAAGTAAATT TAAGGAAGCG ACATGCAAGT GAATCTGTTG GTGATTTATC CCACAGTGAG 
AAAGGGGAGA ATGACAAGGT TATCGAAATC CAAACCGTGC CAGAAGAAAA AGCTGATATA 
CAAAAGGAGC AGCAGGACAT CGTAAAAGGC AGACTTGAAG GGCAGAAAGA AAAGAGAAAT 
GCTGCATCTA CAGTTAAGGA ATCTAAAAGT AACAGAGTCC TGGAAGAAGT GCAAGAGCAT 
GAATGTAAAG TAGTGCCCAG TGAAAAACAA AATAACATGC AATGTATTAG AAATATAGTC 
AATGATTTAT GTAAGGAGGA GGGAGTTGTT AAACTATCAG AACAAATGGC TAAGAGTGTG 
AATAAACAAC CAGCATGTAG GCTTGAAGAA GATGTTGTTA TAAAACAGGC AAAGGTTCTG 
CTATCTGATA TTTTAAGAAA GGATAAACAA TTAATAGATA AGCAATTTCT AGGAGGAACT 
GTAGGAAGAG GATTTTCTAC CTTAGCCTCT AAAATAGAGA GCGCTGAGGA GGAAGAAGAA 
CAGACTGAAA TAAGTGGAGG CAGATCCAAT TGTTCAGTGC AGAGGAGGAT TATAAGACGG 
CGAATGATGA GGCCTAGAGC AGCGAAAACA AAAGCAGAAG GTAAGCAAAG CACCAAGGGA 
CTCTTGAACA AAGAAGTTCC AGATAGAGTA AAGACAGAGT TGAGCGAGGA AACTTTGCAG 
CTTCAGTCTC CACAGATGCA TAACGACCCA CCGGCCACCT GCCTCAACCC ACTACCTCAT 
AAGTCACTAA TTTCAGCAAA AGCCACGACC AATCTGCATC CAGACACCAA TCTGCATCCA 
GACACCAATC CACAGGCACA CATTCAGTCC AACATACCAC TGAAGAAGCG TACATTTCGT 
AGTAATGTGG AAGTAGGTTT GAATCCTGCT TCGGATCGGG CTTCTAAAGA AGCTGCAGAG 
CTAAATTCAT CCACAGAGTT GACGCAGGGC CTAACCACAT GTTCAGATGA GAGTAGCAAA 
GTAAAAAGAG ACTGTAAAGG TAGATGTAGG AGAACAGAGG TCCAGAGGTT AACCACCAAA 
AGGATCACAA CATTTAACCG AGTGCTTCGC TCCAGAGCTA ATGAAGTCCA ACGGAGCCAT 
TTATTCAGGA AAGTCCGAAA GTGTAAATTA GAGAGGCTAG ATGAGGATGA CATTAACAAG 
TCTCAGACAC TACAAAGTGA TCATCTTGCA AATGACAAAC ATGCTGATTA TCCTGCTGAA 
CTCCAACAGC ACTGTGGTAA AAATCCAAAT GAATGTCTCA AGGAAGATAG GGTGGAGACG 
TTAATCCAGC AAAGTGCTTC GGAGGATACG AAGCCAGACC TCAATTTTAA GATTCGTTTT 
AAAAGGAGGA GAGGGAGAGT ATGGGAGATG CAAAGAGCAG GATTTGAAAA TCTGGCGTTA 
AAATCAGAAA AAGGAGACGA GATGACATGC GATCCTTTTA AAGCCATCAT GGATTCGGTG 
TCGGTTTTAA ACATGGAGAT GGAGGCAGCT CAGGCTCATG TGCAGGCTAG TAAGAAGTCA 
AAGCTTAGAC TGCATCGATT GAAAAAAAGA GGTCAGAGGC TCCGTGAAAG GCAAACCGTA 
AACGTCCCCT CACACGTAAA ATCAGACCAA GATCTTGATA ATAGCGAAAA AGTATGTGAC 
GCGCCCAGAG ACAAAGCAGA AATTAAAGAG AAGCTCGACG TAGTCAAGGT GCTGCCAGGA 
AAAATTGAGA GTTGTACAGA TGACGGTCAT GTGTTGTCAG CTCAAGGGCT AGAAACAAAA 
ATTGAAGATC GTTTTGTAAA GAACTGCTGC TTGTTTGAAA GTGGGTCACA GCAGAAACTT 
CAGCCAGAAT TGGACTCAAA TGGTCTTCCA TTACCCGTAA TTAAACTACG AAGAAAGACA 
GAGGATATTT GGGAAGTGGA CAGCAAAGAG GCTCTTCGAC ACACTGATGT TAAAGTAGAG 
CCCAATGTTA AGAAAGAGAT GAAGGGTCAC ATTATCGGGA AACAAAAGAA AGAATGTCTT 
GATTTTAGCA AATTTAAGGA AGAGTGTCCG TCTGCACAGA GACTGAACAG CAACTCAGAC 
CTTCTGAAAA CAGAACCACC ACCGTTTTCC TTATCTCTGT CACCACTGTC TCTGTCTTCC 
CCTCTTAGCG ACAATAGAAC TGAAGTTATA ACCACGGCTG CAGATACAGT CATTGAAAGG 
CCAGAGATGA ACAGCGGAGG AAGGAAACAG AGGCATAAAA TGGAGAGAAC ACGCAAGTGT 
GGGCCTGAAG AAACACCCAC CACTTGTCTT AGTCACACTC TCCAACAAAT TGACAACAGT 
CTCTCTCGGC TTTCCGAGGG CTTGTGTTCA TCTCAGACTT TGGAAAAGCC GACCGCCTGT 
TCCAGTGCTT CTAATTCTGT CATCCAGCCC CCGTCCCAGT CTCCTCCATT CACAACTGGA 
GATAACATGC TTTCTGGTGA GCCCAGCTTC ACAAACTGCT GTGATGACAT CCTGGACTTT 
CAGTGTCTCA ACTTTGAGGG GTATTATCAG CCACAGAATA TACTTCCATC TTCCCCATCA 
GATCTTTGTT CATTGGATCC ACCCACAGAT CCTTTCAGCA GTCCTCTCTC TCACAGCCCT 
TCTGATACAT GGACCACTGA AACGCCATAC CTTGGCCCTC CCAGTCCAGG AAATAACTTT 
ACCAGTGAGG ATCTGCAGTT TTTTCCAGGT CTAATTTCTT CCAAAGGTGA CTCTGTTCCT 
CTGGAATGTG ACGCGAGGGA TACCTCCAAA GACAGAAACC TACCAAACCC CAGTTTCAGT 
TTTTCAACAC TTGGCAACTC TGACCTAACT GCTAAGGATC GGATTATAAG TAAAAATCCA 
GGAATGAGGC TCTGCAGAGA AACTCAGCCC ATGTCTGTAG TCAACAAGCC TCGGCTGTTT 
GGTACAACAT CATCTGTTAC ATCTCAAAAT CCTCTTACTT TGACCCAACA GCCCATCAAC 
ATCAAGGCGA GCACTAGTCA ATCAAAAATC CAGTCTAATC TCAGAACCCA GGGCCCCTTC 
CATCGTATGA CCATTCCTAG TAAATCTCAG CCTTTTGCAA GTAGTCAGCC AAATGCAGTC 
AGAGGAGTGT CCCAGAGTTG CTTGCCTAAA TCCGTCCCAT CATCTCAAAT CCCTAACAAA 
TTTGTTTCTC CTCAGCTTTT CACTGTGAGA AATCCCAATC CTCCTGACAA TCCATTCAAA 
GCCCATGAGA AACCATCTTC AGTCATTCAC AGGGTGCTTA AGTTTCAGGG GGGGAACCAG 
AGTCAGAACT TGTATAGTGC ACCCTGCAAA GACACCCTGG CTGCTGGCAG TCCAACTATC 
ACATCCCGAA CACCCATCTC CAGATCTGGT GCTATTGAGA AGCAGAGTCA AAGTCAGAAA 
CTAGGTGTTT GCACAGATGG TCATCACAAA GGCAACATAA CTGTTCAAGG ATTTGCAAAG 
GATGTCGACC ATCTTGGTCC CTCCAGCCAC CCCAGCAGGT CACACCCTCA TTTCAACAAA 
CTCAACTCTT TTGCTCAGTC CTTTAGTAAA AGTAAGATGT CTTCTGAAAA ACATGAAAAT 
GTTGAAACCA ACATGACATA CAAAAACCTT TCAGCGCTAC CAAGACCCTT CTTCTTTCCA 
AGCAAAATGT CTGACGGTTA TTCATCGCTA CAAGACAAGA GTTTAAGACA GGATAAATCC 
TCAACATCTA GCTCAGACAA GCCCCATCCA TGTTACACTC AGCAAGACCC TTTTGATTTT 
AGCTTCGGCT CGTCTCTTTC ACCCATGTCT CAGCACAACA GCCCCCAAGT GATCCACAGT 
ACACCTCCTG GTACGCCAGC ACCAGCAAAC AAGAGTCAGT CCTCAGCCTC TTTCCCTTAT 
GGCTATCAAG GCCCTCCTTA CGTACTAAAC TTCAGTGGAG ATCATTCATT GACCCTGGGT 
CTCAGGGATG GGGCTGATGG TTACCCAGGT TTAGGCTCGG CAAACTACAC CTACCACTGC 
CTGATGGAAC CTTCAGGCAC CCAAGGCCGG CTGGTTCTCG AGCCCTGTGA ACCCCAGTTA 
TCTAACCCAG CATCCTTTTC CTTGGGTGGC TTTTCTGGAC TCAAAGGTCA GGATGAACTT 
TACAGAAAGG ATATGCAGCA ACAGTGCCAG CCTGGGGAGC ACCAAGGCCC ACCACACTAT 
GGACCCGTAC CATCGTCTCA CTCCATGGGT GCTACAAAAC CCAAGAGAGT GAGGTTAGTG 
GTGACAGATG GCACAGTAGA CTTAGATCTA CAGTACTCGG ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104930715 ( XM_019256105.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019111650.1 Larimichthys crocea histone-lysine N-methyltransferase KMT5B-like (LOC104930715), transcript variant X2, mRNA.

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CloneID	OLa86931
Clone ID Related Accession (Same CDS sequence)	XM_019256105.1
Accession Version	XM_019256105.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	uncharacterized protein LOC104930715 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017608059.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGGAT GCACTAGAAT GAGTGTGAAG GAGCTGTGTG AGACCGATGA CCTGGCCACC 
GGTTTGGTGC TGGACCCCCT GCTGGGCTTC AGCACCCACA AAATGAACAT CAGCCCCCCT 
CCTGAGATCC GGCGTTGGGG TAATCTCAAA GAGACCCTGT TGCGCTTTCA GCGTACGCAT 
GACTTCAATG CTACGTTTGA GGCGCTGACG GTGGGCGAGC TGGCTGGTGA TTACTTCAGT 
GCTTTGGGAA GTCACCGTCA GGAGCTGCTG AGGCAACATG TGTATCGCTA CCTCAGTGCC 
TTCCTGTTGG ATAGCGGTAT CAAGATAGAG TCCTGTGACC GATACTCCTC AGAGACCAAT 
GGAGCAAAGA TAACCTCAAC AAGGCACTGG TTTGCAGGAG AGCGGGTGGA GGTCCTGCTG 
GGCTGCATAG CAGAGCTGAG CCCCGCTGAC AGTGCCGTGC TGAGGACGGG AGTCAATGAC 
TTCAGTGTGA TGTATTCCAC GCGCAAACGC TGCGCTCAGC TCTGGCTCGG GCCTGCAGCT 
TTCATTAACC ATGACTGCAA ACCAAACTGC AAGTTCGTCC CTGGTGAGAA GAATATCGCT 
TGTGTTGAAG CCATTCGACC TATCTCACCT GGGGAGGAGA TCACATGTTA CTATGGTGCC 
AGCTTTTTTG GGGAAGGAAA TGAAATGTGT GAATGCTGCA CCTGTGAGAG GAACGGAGAG 
GGCCACTTCA AACACAGAGG GAAGCAGCCT GAATGTGAAG AAACAAAGGA CCCCGTGGGC 
CAAAAGTACC GTCTGAGAGA GCGATATCTT CGTCATCACA GAGAGAAGGG TCACTTTCCT 
ACACGGCAGA TAATACCAGG ATTACACTCA GCCATCCCCT CAAGGAATTC CTTTACCCAG 
CAGATGAGGA GAAATGCCTT GAAGAACAGA AAATTTAGCA AATCAAAGAA GTGGAGACGG 
GAGCACAGAC GATCAGGAAA GCACCCGCTG ACATCCTCCA CGGCCAACCA GTGTCGGACA 
GTTCCACTTT TATCCCAAGT TACTCTGAGG GACCTCAGAA TCAGAGTACG GCGCCACTCA 
GTGGATTTCT TACTCCATTG TAAGGATCCT AAAAGCAAAG AAAGGGCTCT GCTTCAACAA 
CTGGAGGAAG TGAAGCCCCT GAGTGAACTG AATACGACTG GTGAAGGGAA TAACAACAGT 
GAGGTAAACT TGAAGCCCTT CACTCTAAAT TCCCCCATGC CTGTTCATCA GAAAAGTAGA 
CATGCAGGAG GCAGCGCAGG CCTTAATGGA AAGTCTGTTA AAGTGGTGGA TAGGCAGGTG 
GCTCTGAGGA CATCTTCGAG AACAAGAAGC ATGGTTCACA ACACGGTGAC ACATCAAACA 
GTGTCCAGCA ACACAGCCAA CACTAAGACT GTTTGTCACC CTGTTCAAAT TGACAGAACC 
TGCAATGCAA TAACAAGCGA GAAGACTGAA CAAGCAATGA CCGAGAATCA AAGCAACAAG 
TGCAGCAGCG GCGTTGAATC CTGCATCATC GACGTGGATC AAAATCCTGC TGAGCAAACC 
GAGCACAAGA CCAGCGTAGC GTCATCCGTG TTAGAAGTTT GTCAGAGTGT CACAGATAGA 
TCGAAGAAAG CCGGAGACAA TCCATTAATA TCACTGAAGC AATACCTTAC AGTCAACCTA 
ATAAGGTTAT CAGTGCCACA GAGTCTAGAA GCAGAAAAGA CTCCTGAAAA CAAGAGAGGA 
AGCGTGGTGC TAAGAGGAAG GAGCAGGCAG AGTCTTAGAC CAGCGGCCAT GACAGCACAG 
TCAGAAAGAA GAGTGTCCAG ATCTCAGCAG AGGCAGGGAG GAAGTTCAGA GATCTGCACA 
TCTGAAAGCA ACAGAGGGGT GAGAGAAATG TTTTTCAAGG CCATTAATAA AGTAAATTTA 
AGGAAGCGAC ATGCAAGTGA ATCTGTTGGT GATTTATCCC ACAGTGAGAA AGGGGAGAAT 
GACAAGGTTA TCGAAATCCA AACCGTGCCA GAAGAAAAAG CTGATATACA AAAGGAGCAG 
CAGGACATCG TAAAAGGCAG ACTTGAAGGG CAGAAAGAAA AGAGAAATGC TGCATCTACA 
GTTAAGGAAT CTAAAAGTAA CAGAGTCCTG GAAGAAGTGC AAGAGCATGA ATGTAAAGTA 
GTGCCCAGTG AAAAACAAAA TAACATGCAA TGTATTAGAA ATATAGTCAA TGATTTATGT 
AAGGAGGAGG GAGTTGTTAA ACTATCAGAA CAAATGGCTA AGAGTGTGAA TAAACAACCA 
GCATGTAGGC TTGAAGAAGA TGTTGTTATA AAACAGGCAA AGGTTCTGCT ATCTGATATT 
TTAAGAAAGG ATAAACAATT AATAGATAAG CAATTTCTAG GAGGAACTGT AGGAAGAGGA 
TTTTCTACCT TAGCCTCTAA AATAGAGAGC GCTGAGGAGG AAGAAGAACA GACTGAAATA 
AGTGGAGGCA GATCCAATTG TTCAGTGCAG AGGAGGATTA TAAGACGGCG AATGATGAGG 
CCTAGAGCAG CGAAAACAAA AGCAGAAGGT AAGCAAAGCA CCAAGGGACT CTTGAACAAA 
GAAGTTCCAG ATAGAGTAAA GACAGAGTTG AGCGAGGAAA CTTTGCAGCT TCAGTCTCCA 
CAGATGCATA ACGACCCACC GGCCACCTGC CTCAACCCAC TACCTCATAA GTCACTAATT 
TCAGCAAAAG CCACGACCAA TCTGCATCCA GACACCAATC TGCATCCAGA CACCAATCCA 
CAGGCACACA TTCAGTCCAA CATACCACTG AAGAAGCGTA CATTTCGTAG TAATGTGGAA 
GTAGGTTTGA ATCCTGCTTC GGATCGGGCT TCTAAAGAAG CTGCAGAGCT AAATTCATCC 
ACAGAGTTGA CGCAGGGCCT AACCACATGT TCAGATGAGA GTAGCAAAGT AAAAAGAGAC 
TGTAAAGGTA GATGTAGGAG AACAGAGGTC CAGAGGTTAA CCACCAAAAG GATCACAACA 
TTTAACCGAG TGCTTCGCTC CAGAGCTAAT GAAGTCCAAC GGAGCCATTT ATTCAGGAAA 
GTCCGAAAGT GTAAATTAGA GAGGCTAGAT GAGGATGACA TTAACAAGTC TCAGACACTA 
CAAAGTGATC ATCTTGCAAA TGACAAACAT GCTGATTATC CTGCTGAACT CCAACAGCAC 
TGTGGTAAAA ATCCAAATGA ATGTCTCAAG GAAGATAGGG TGGAGACGTT AATCCAGCAA 
AGTGCTTCGG AGGATACGAA GCCAGACCTC AATTTTAAGA TTCGTTTTAA AAGGAGGAGA 
GGGAGAGTAT GGGAGATGCA AAGAGCAGGA TTTGAAAATC TGGCGTTAAA ATCAGAAAAA 
GGAGACGAGA TGACATGCGA TCCTTTTAAA GCCATCATGG ATTCGGTGTC GGTTTTAAAC 
ATGGAGATGG AGGCAGCTCA GGCTCATGTG CAGGCTAGTA AGAAGTCAAA GCTTAGACTG 
CATCGATTGA AAAAAAGAGG TCAGAGGCTC CGTGAAAGGC AAACCGTAAA CGTCCCCTCA 
CACGTAAAAT CAGACCAAGA TCTTGATAAT AGCGAAAAAG TATGTGACGC GCCCAGAGAC 
AAAGCAGAAA TTAAAGAGAA GCTCGACGTA GTCAAGGTGC TGCCAGGAAA AATTGAGAGT 
TGTACAGATG ACGGTCATGT GTTGTCAGCT CAAGGGCTAG AAACAAAAAT TGAAGATCGT 
TTTGTAAAGA ACTGCTGCTT GTTTGAAAGT GGGTCACAGC AGAAACTTCA GCCAGAATTG 
GACTCAAATG GTCTTCCATT ACCCGTAATT AAACTACGAA GAAAGACAGA GGATATTTGG 
GAAGTGGACA GCAAAGAGGC TCTTCGACAC ACTGATGTTA AAGTAGAGCC CAATGTTAAG 
AAAGAGATGA AGGGTCACAT TATCGGGAAA CAAAAGAAAG AATGTCTTGA TTTTAGCAAA 
TTTAAGGAAG AGTGTCCGTC TGCACAGAGA CTGAACAGCA ACTCAGACCT TCTGAAAACA 
GAACCACCAC CGTTTTCCTT ATCTCTGTCA CCACTGTCTC TGTCTTCCCC TCTTAGCGAC 
AATAGAACTG AAGTTATAAC CACGGCTGCA GATACAGTCA TTGAAAGGCC AGAGATGAAC 
AGCGGAGGAA GGAAACAGAG GCATAAAATG GAGAGAACAC GCAAGTGTGG GCCTGAAGAA 
ACACCCACCA CTTGTCTTAG TCACACTCTC CAACAAATTG ACAACAGTCT CTCTCGGCTT 
TCCGAGGGCT TGTGTTCATC TCAGACTTTG GAAAAGCCGA CCGCCTGTTC CAGTGCTTCT 
AATTCTGTCA TCCAGCCCCC GTCCCAGTCT CCTCCATTCA CAACTGGAGA TAACATGCTT 
TCTGGTGAGC CCAGCTTCAC AAACTGCTGT GATGACATCC TGGACTTTCA GTGTCTCAAC 
TTTGAGGGGT ATTATCAGCC ACAGAATATA CTTCCATCTT CCCCATCAGA TCTTTGTTCA 
TTGGATCCAC CCACAGATCC TTTCAGCAGT CCTCTCTCTC ACAGCCCTTC TGATACATGG 
ACCACTGAAA CGCCATACCT TGGCCCTCCC AGTCCAGGAA ATAACTTTAC CAGTGAGGAT 
CTGCAGTTTT TTCCAGGTCT AATTTCTTCC AAAGGTGACT CTGTTCCTCT GGAATGTGAC 
GCGAGGGATA CCTCCAAAGA CAGAAACCTA CCAAACCCCA GTTTCAGTTT TTCAACACTT 
GGCAACTCTG ACCTAACTGC TAAGGATCGG ATTATAAGTA AAAATCCAGG AATGAGGCTC 
TGCAGAGAAA CTCAGCCCAT GTCTGTAGTC AACAAGCCTC GGCTGTTTGG TACAACATCA 
TCTGTTACAT CTCAAAATCC TCTTACTTTG ACCCAACAGC CCATCAACAT CAAGGCGAGC 
ACTAGTCAAT CAAAAATCCA GTCTAATCTC AGAACCCAGG GCCCCTTCCA TCGTATGACC 
ATTCCTAGTA AATCTCAGCC TTTTGCAAGT AGTCAGCCAA ATGCAGTCAG AGGAGTGTCC 
CAGAGTTGCT TGCCTAAATC CGTCCCATCA TCTCAAATCC CTAACAAATT TGTTTCTCCT 
CAGCTTTTCA CTGTGAGAAA TCCCAATCCT CCTGACAATC CATTCAAAGC CCATGAGAAA 
CCATCTTCAG TCATTCACAG GGTGCTTAAG TTTCAGGGGG GGAACCAGAG TCAGAACTTG 
TATAGTGCAC CCTGCAAAGA CACCCTGGCT GCTGGCAGTC CAACTATCAC ATCCCGAACA 
CCCATCTCCA GATCTGGTGC TATTGAGAAG CAGAGTCAAA GTCAGAAACT AGGTGTTTGC 
ACAGATGGTC ATCACAAAGG CAACATAACT GTTCAAGGAT TTGCAAAGGA TGTCGACCAT 
CTTGGTCCCT CCAGCCACCC CAGCAGGTCA CACCCTCATT TCAACAAACT CAACTCTTTT 
GCTCAGTCCT TTAGTAAAAG TAAGATGTCT TCTGAAAAAC ATGAAAATGT TGAAACCAAC 
ATGACATACA AAAACCTTTC AGCGCTACCA AGACCCTTCT TCTTTCCAAG CAAAATGTCT 
GACGGTTATT CATCGCTACA AGACAAGAGT TTAAGACAGG ATAAATCCTC AACATCTAGC 
TCAGACAAGC CCCATCCATG TTACACTCAG CAAGACCCTT TTGATTTTAG CTTCGGCTCG 
TCTCTTTCAC CCATGTCTCA GCACAACAGC CCCCAAGTGA TCCACAGTAC ACCTCCTGGT 
ACGCCAGCAC CAGCAAACAA GAGTCAGTCC TCAGCCTCTT TCCCTTATGG CTATCAAGGC 
CCTCCTTACG TACTAAACTT CAGTGGAGAT CATTCATTGA CCCTGGGTCT CAGGGATGGG 
GCTGATGGTT ACCCAGGTTT AGGCTCGGCA AACTACACCT ACCACTGCCT GATGGAACCT 
TCAGGCACCC AAGGCCGGCT GGTTCTCGAG CCCTGTGAAC CCCAGTTATC TAACCCAGCA 
TCCTTTTCCT TGGGTGGCTT TTCTGGACTC AAAGGTCAGG ATGAACTTTA CAGAAAGGAT 
ATGCAGCAAC AGTGCCAGCC TGGGGAGCAC CAAGGCCCAC CACACTATGG ACCCGTACCA 
TCGTCTCACT CCATGGGTGC TACAAAACCC AAGAGAGTGA GGTTAGTGGT GACAGATGGC 
ACAGTAGACT TAGATCTACA GTACTCGGAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019111650.1
CDS	406..6618
Translation	MDGCTRMSVKELCETDDLATGLVLDPLLGFSTHKMNISPPPEIRRWGNLKETLLRFQRTHDFNATFEALTVGELAGDYFSALGSHRQELLRQHVYRYLSAFLLDSGIKIESCDRYSSETNGAKITSTRHWFAGERVEVLLGCIAELSPADSAVLRTGVNDFSVMYSTRKRCAQLWLGPAAFINHDCKPNCKFVPGEKNIACVEAIRPISPGEEITCYYGASFFGEGNEMCECCTCERNGEGHFKHRGKQPECEETKDPVGQKYRLRERYLRHHREKGHFPTRQIIPGLHSAIPSRNSFTQQMRRNALKNRKFSKSKKWRREHRRSGKHPLTSSTANQCRTVPLLSQVTLRDLRIRVRRHSVDFLLHCKDPKSKERALLQQLEEVKPLSELNTTGEGNNNSEVNLKPFTLNSPMPVHQKSRHAGGSAGLNGKSVKVVDRQVALRTSSRTRSMVHNTVTHQTVSSNTANTKTVCHPVQIDRTCNAITSEKTEQAMTENQSNKCSSGVESCIIDVDQNPAEQTEHKTSVASSVLEVCQSVTDRSKKAGDNPLISLKQYLTVNLIRLSVPQSLEAEKTPENKRGSVVLRGRSRQSLRPAAMTAQSERRVSRSQQRQGGSSEICTSESNRGVREMFFKAINKVNLRKRHASESVGDLSHSEKGENDKVIEIQTVPEEKADIQKEQQDIVKGRLEGQKEKRNAASTVKESKSNRVLEEVQEHECKVVPSEKQNNMQCIRNIVNDLCKEEGVVKLSEQMAKSVNKQPACRLEEDVVIKQAKVLLSDILRKDKQLIDKQFLGGTVGRGFSTLASKIESAEEEEEQTEISGGRSNCSVQRRIIRRRMMRPRAAKTKAEGKQSTKGLLNKEVPDRVKTELSEETLQLQSPQMHNDPPATCLNPLPHKSLISAKATTNLHPDTNLHPDTNPQAHIQSNIPLKKRTFRSNVEVGLNPASDRASKEAAELNSSTELTQGLTTCSDESSKVKRDCKGRCRRTEVQRLTTKRITTFNRVLRSRANEVQRSHLFRKVRKCKLERLDEDDINKSQTLQSDHLANDKHADYPAELQQHCGKNPNECLKEDRVETLIQQSASEDTKPDLNFKIRFKRRRGRVWEMQRAGFENLALKSEKGDEMTCDPFKAIMDSVSVLNMEMEAAQAHVQASKKSKLRLHRLKKRGQRLRERQTVNVPSHVKSDQDLDNSEKVCDAPRDKAEIKEKLDVVKVLPGKIESCTDDGHVLSAQGLETKIEDRFVKNCCLFESGSQQKLQPELDSNGLPLPVIKLRRKTEDIWEVDSKEALRHTDVKVEPNVKKEMKGHIIGKQKKECLDFSKFKEECPSAQRLNSNSDLLKTEPPPFSLSLSPLSLSSPLSDNRTEVITTAADTVIERPEMNSGGRKQRHKMERTRKCGPEETPTTCLSHTLQQIDNSLSRLSEGLCSSQTLEKPTACSSASNSVIQPPSQSPPFTTGDNMLSGEPSFTNCCDDILDFQCLNFEGYYQPQNILPSSPSDLCSLDPPTDPFSSPLSHSPSDTWTTETPYLGPPSPGNNFTSEDLQFFPGLISSKGDSVPLECDARDTSKDRNLPNPSFSFSTLGNSDLTAKDRIISKNPGMRLCRETQPMSVVNKPRLFGTTSSVTSQNPLTLTQQPINIKASTSQSKIQSNLRTQGPFHRMTIPSKSQPFASSQPNAVRGVSQSCLPKSVPSSQIPNKFVSPQLFTVRNPNPPDNPFKAHEKPSSVIHRVLKFQGGNQSQNLYSAPCKDTLAAGSPTITSRTPISRSGAIEKQSQSQKLGVCTDGHHKGNITVQGFAKDVDHLGPSSHPSRSHPHFNKLNSFAQSFSKSKMSSEKHENVETNMTYKNLSALPRPFFFPSKMSDGYSSLQDKSLRQDKSSTSSSDKPHPCYTQQDPFDFSFGSSLSPMSQHNSPQVIHSTPPGTPAPANKSQSSASFPYGYQGPPYVLNFSGDHSLTLGLRDGADGYPGLGSANYTYHCLMEPSGTQGRLVLEPCEPQLSNPASFSLGGFSGLKGQDELYRKDMQQQCQPGEHQGPPHYGPVPSSHSMGATKPKRVRLVVTDGTVDLDLQYSD

Target ORF information:

RefSeq Version	XM_019256105.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea histone-lysine N-methyltransferase KMT5B-like (LOC104930715), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019256105.1

ORF Insert Sequence:

ATGGACGGAT GCACTAGAAT GAGTGTGAAG GAGCTGTGTG AGACCGATGA CCTGGCCACC 
GGTTTGGTGC TGGACCCCCT GCTGGGCTTC AGCACCCACA AAATGAACAT CAGCCCCCCT 
CCTGAGATCC GGCGTTGGGG TAATCTCAAA GAGACCCTGT TGCGCTTTCA GCGTACGCAT 
GACTTCAATG CTACGTTTGA GGCGCTGACG GTGGGCGAGC TGGCTGGTGA TTACTTCAGT 
GCTTTGGGAA GTCACCGTCA GGAGCTGCTG AGGCAACATG TGTATCGCTA CCTCAGTGCC 
TTCCTGTTGG ATAGCGGTAT CAAGATAGAG TCCTGTGACC GATACTCCTC AGAGACCAAT 
GGAGCAAAGA TAACCTCAAC AAGGCACTGG TTTGCAGGAG AGCGGGTGGA GGTCCTGCTG 
GGCTGCATAG CAGAGCTGAG CCCCGCTGAC AGTGCCGTGC TGAGGACGGG AGTCAATGAC 
TTCAGTGTGA TGTATTCCAC GCGCAAACGC TGCGCTCAGC TCTGGCTCGG GCCTGCAGCT 
TTCATTAACC ATGACTGCAA ACCAAACTGC AAGTTCGTCC CTGGTGAGAA GAATATCGCT 
TGTGTTGAAG CCATTCGACC TATCTCACCT GGGGAGGAGA TCACATGTTA CTATGGTGCC 
AGCTTTTTTG GGGAAGGAAA TGAAATGTGT GAATGCTGCA CCTGTGAGAG GAACGGAGAG 
GGCCACTTCA AACACAGAGG GAAGCAGCCT GAATGTGAAG AAACAAAGGA CCCCGTGGGC 
CAAAAGTACC GTCTGAGAGA GCGATATCTT CGTCATCACA GAGAGAAGGG TCACTTTCCT 
ACACGGCAGA TAATACCAGG ATTACACTCA GCCATCCCCT CAAGGAATTC CTTTACCCAG 
CAGATGAGGA GAAATGCCTT GAAGAACAGA AAATTTAGCA AATCAAAGAA GTGGAGACGG 
GAGCACAGAC GATCAGGAAA GCACCCGCTG ACATCCTCCA CGGCCAACCA GTGTCGGACA 
GTTCCACTTT TATCCCAAGT TACTCTGAGG GACCTCAGAA TCAGAGTACG GCGCCACTCA 
GTGGATTTCT TACTCCATTG TAAGGATCCT AAAAGCAAAG AAAGGGCTCT GCTTCAACAA 
CTGGAGGAAG TGAAGCCCCT GAGTGAACTG AATACGACTG GTGAAGGGAA TAACAACAGT 
GAGGTAAACT TGAAGCCCTT CACTCTAAAT TCCCCCATGC CTGTTCATCA GAAAAGTAGA 
CATGCAGGAG GCAGCGCAGG CCTTAATGGA AAGTCTGTTA AAGTGGTGGA TAGGCAGGTG 
GCTCTGAGGA CATCTTCGAG AACAAGAAGC ATGGTTCACA ACACGGTGAC ACATCAAACA 
GTGTCCAGCA ACACAGCCAA CACTAAGACT GTTTGTCACC CTGTTCAAAT TGACAGAACC 
TGCAATGCAA TAACAAGCGA GAAGACTGAA CAAGCAATGA CCGAGAATCA AAGCAACAAG 
TGCAGCAGCG GCGTTGAATC CTGCATCATC GACGTGGATC AAAATCCTGC TGAGCAAACC 
GAGCACAAGA CCAGCGTAGC GTCATCCGTG TTAGAAGTTT GTCAGAGTGT CACAGATAGA 
TCGAAGAAAG CCGGAGACAA TCCATTAATA TCACTGAAGC AATACCTTAC AGTCAACCTA 
ATAAGGTTAT CAGTGCCACA GAGTCTAGAA GCAGAAAAGA CTCCTGAAAA CAAGAGAGGA 
AGCGTGGTGC TAAGAGGAAG GAGCAGGCAG AGTCTTAGAC CAGCGGCCAT GACAGCACAG 
TCAGAAAGAA GAGTGTCCAG ATCTCAGCAG AGGCAGGGAG GAAGTTCAGA GATCTGCACA 
TCTGAAAGCA ACAGAGGGGT GAGAGAAATG TTTTTCAAGG CCATTAATAA AGTAAATTTA 
AGGAAGCGAC ATGCAAGTGA ATCTGTTGGT GATTTATCCC ACAGTGAGAA AGGGGAGAAT 
GACAAGGTTA TCGAAATCCA AACCGTGCCA GAAGAAAAAG CTGATATACA AAAGGAGCAG 
CAGGACATCG TAAAAGGCAG ACTTGAAGGG CAGAAAGAAA AGAGAAATGC TGCATCTACA 
GTTAAGGAAT CTAAAAGTAA CAGAGTCCTG GAAGAAGTGC AAGAGCATGA ATGTAAAGTA 
GTGCCCAGTG AAAAACAAAA TAACATGCAA TGTATTAGAA ATATAGTCAA TGATTTATGT 
AAGGAGGAGG GAGTTGTTAA ACTATCAGAA CAAATGGCTA AGAGTGTGAA TAAACAACCA 
GCATGTAGGC TTGAAGAAGA TGTTGTTATA AAACAGGCAA AGGTTCTGCT ATCTGATATT 
TTAAGAAAGG ATAAACAATT AATAGATAAG CAATTTCTAG GAGGAACTGT AGGAAGAGGA 
TTTTCTACCT TAGCCTCTAA AATAGAGAGC GCTGAGGAGG AAGAAGAACA GACTGAAATA 
AGTGGAGGCA GATCCAATTG TTCAGTGCAG AGGAGGATTA TAAGACGGCG AATGATGAGG 
CCTAGAGCAG CGAAAACAAA AGCAGAAGGT AAGCAAAGCA CCAAGGGACT CTTGAACAAA 
GAAGTTCCAG ATAGAGTAAA GACAGAGTTG AGCGAGGAAA CTTTGCAGCT TCAGTCTCCA 
CAGATGCATA ACGACCCACC GGCCACCTGC CTCAACCCAC TACCTCATAA GTCACTAATT 
TCAGCAAAAG CCACGACCAA TCTGCATCCA GACACCAATC TGCATCCAGA CACCAATCCA 
CAGGCACACA TTCAGTCCAA CATACCACTG AAGAAGCGTA CATTTCGTAG TAATGTGGAA 
GTAGGTTTGA ATCCTGCTTC GGATCGGGCT TCTAAAGAAG CTGCAGAGCT AAATTCATCC 
ACAGAGTTGA CGCAGGGCCT AACCACATGT TCAGATGAGA GTAGCAAAGT AAAAAGAGAC 
TGTAAAGGTA GATGTAGGAG AACAGAGGTC CAGAGGTTAA CCACCAAAAG GATCACAACA 
TTTAACCGAG TGCTTCGCTC CAGAGCTAAT GAAGTCCAAC GGAGCCATTT ATTCAGGAAA 
GTCCGAAAGT GTAAATTAGA GAGGCTAGAT GAGGATGACA TTAACAAGTC TCAGACACTA 
CAAAGTGATC ATCTTGCAAA TGACAAACAT GCTGATTATC CTGCTGAACT CCAACAGCAC 
TGTGGTAAAA ATCCAAATGA ATGTCTCAAG GAAGATAGGG TGGAGACGTT AATCCAGCAA 
AGTGCTTCGG AGGATACGAA GCCAGACCTC AATTTTAAGA TTCGTTTTAA AAGGAGGAGA 
GGGAGAGTAT GGGAGATGCA AAGAGCAGGA TTTGAAAATC TGGCGTTAAA ATCAGAAAAA 
GGAGACGAGA TGACATGCGA TCCTTTTAAA GCCATCATGG ATTCGGTGTC GGTTTTAAAC 
ATGGAGATGG AGGCAGCTCA GGCTCATGTG CAGGCTAGTA AGAAGTCAAA GCTTAGACTG 
CATCGATTGA AAAAAAGAGG TCAGAGGCTC CGTGAAAGGC AAACCGTAAA CGTCCCCTCA 
CACGTAAAAT CAGACCAAGA TCTTGATAAT AGCGAAAAAG TATGTGACGC GCCCAGAGAC 
AAAGCAGAAA TTAAAGAGAA GCTCGACGTA GTCAAGGTGC TGCCAGGAAA AATTGAGAGT 
TGTACAGATG ACGGTCATGT GTTGTCAGCT CAAGGGCTAG AAACAAAAAT TGAAGATCGT 
TTTGTAAAGA ACTGCTGCTT GTTTGAAAGT GGGTCACAGC AGAAACTTCA GCCAGAATTG 
GACTCAAATG GTCTTCCATT ACCCGTAATT AAACTACGAA GAAAGACAGA GGATATTTGG 
GAAGTGGACA GCAAAGAGGC TCTTCGACAC ACTGATGTTA AAGTAGAGCC CAATGTTAAG 
AAAGAGATGA AGGGTCACAT TATCGGGAAA CAAAAGAAAG AATGTCTTGA TTTTAGCAAA 
TTTAAGGAAG AGTGTCCGTC TGCACAGAGA CTGAACAGCA ACTCAGACCT TCTGAAAACA 
GAACCACCAC CGTTTTCCTT ATCTCTGTCA CCACTGTCTC TGTCTTCCCC TCTTAGCGAC 
AATAGAACTG AAGTTATAAC CACGGCTGCA GATACAGTCA TTGAAAGGCC AGAGATGAAC 
AGCGGAGGAA GGAAACAGAG GCATAAAATG GAGAGAACAC GCAAGTGTGG GCCTGAAGAA 
ACACCCACCA CTTGTCTTAG TCACACTCTC CAACAAATTG ACAACAGTCT CTCTCGGCTT 
TCCGAGGGCT TGTGTTCATC TCAGACTTTG GAAAAGCCGA CCGCCTGTTC CAGTGCTTCT 
AATTCTGTCA TCCAGCCCCC GTCCCAGTCT CCTCCATTCA CAACTGGAGA TAACATGCTT 
TCTGGTGAGC CCAGCTTCAC AAACTGCTGT GATGACATCC TGGACTTTCA GTGTCTCAAC 
TTTGAGGGGT ATTATCAGCC ACAGAATATA CTTCCATCTT CCCCATCAGA TCTTTGTTCA 
TTGGATCCAC CCACAGATCC TTTCAGCAGT CCTCTCTCTC ACAGCCCTTC TGATACATGG 
ACCACTGAAA CGCCATACCT TGGCCCTCCC AGTCCAGGAA ATAACTTTAC CAGTGAGGAT 
CTGCAGTTTT TTCCAGGTCT AATTTCTTCC AAAGGTGACT CTGTTCCTCT GGAATGTGAC 
GCGAGGGATA CCTCCAAAGA CAGAAACCTA CCAAACCCCA GTTTCAGTTT TTCAACACTT 
GGCAACTCTG ACCTAACTGC TAAGGATCGG ATTATAAGTA AAAATCCAGG AATGAGGCTC 
TGCAGAGAAA CTCAGCCCAT GTCTGTAGTC AACAAGCCTC GGCTGTTTGG TACAACATCA 
TCTGTTACAT CTCAAAATCC TCTTACTTTG ACCCAACAGC CCATCAACAT CAAGGCGAGC 
ACTAGTCAAT CAAAAATCCA GTCTAATCTC AGAACCCAGG GCCCCTTCCA TCGTATGACC 
ATTCCTAGTA AATCTCAGCC TTTTGCAAGT AGTCAGCCAA ATGCAGTCAG AGGAGTGTCC 
CAGAGTTGCT TGCCTAAATC CGTCCCATCA TCTCAAATCC CTAACAAATT TGTTTCTCCT 
CAGCTTTTCA CTGTGAGAAA TCCCAATCCT CCTGACAATC CATTCAAAGC CCATGAGAAA 
CCATCTTCAG TCATTCACAG GGTGCTTAAG TTTCAGGGGG GGAACCAGAG TCAGAACTTG 
TATAGTGCAC CCTGCAAAGA CACCCTGGCT GCTGGCAGTC CAACTATCAC ATCCCGAACA 
CCCATCTCCA GATCTGGTGC TATTGAGAAG CAGAGTCAAA GTCAGAAACT AGGTGTTTGC 
ACAGATGGTC ATCACAAAGG CAACATAACT GTTCAAGGAT TTGCAAAGGA TGTCGACCAT 
CTTGGTCCCT CCAGCCACCC CAGCAGGTCA CACCCTCATT TCAACAAACT CAACTCTTTT 
GCTCAGTCCT TTAGTAAAAG TAAGATGTCT TCTGAAAAAC ATGAAAATGT TGAAACCAAC 
ATGACATACA AAAACCTTTC AGCGCTACCA AGACCCTTCT TCTTTCCAAG CAAAATGTCT 
GACGGTTATT CATCGCTACA AGACAAGAGT TTAAGACAGG ATAAATCCTC AACATCTAGC 
TCAGACAAGC CCCATCCATG TTACACTCAG CAAGACCCTT TTGATTTTAG CTTCGGCTCG 
TCTCTTTCAC CCATGTCTCA GCACAACAGC CCCCAAGTGA TCCACAGTAC ACCTCCTGGT 
ACGCCAGCAC CAGCAAACAA GAGTCAGTCC TCAGCCTCTT TCCCTTATGG CTATCAAGGC 
CCTCCTTACG TACTAAACTT CAGTGGAGAT CATTCATTGA CCCTGGGTCT CAGGGATGGG 
GCTGATGGTT ACCCAGGTTT AGGCTCGGCA AACTACACCT ACCACTGCCT GATGGAACCT 
TCAGGCACCC AAGGCCGGCT GGTTCTCGAG CCCTGTGAAC CCCAGTTATC TAACCCAGCA 
TCCTTTTCCT TGGGTGGCTT TTCTGGACTC AAAGGTCAGG ATGAACTTTA CAGAAAGGAT 
ATGCAGCAAC AGTGCCAGCC TGGGGAGCAC CAAGGCCCAC CACACTATGG ACCCGTACCA 
TCGTCTCACT CCATGGGTGC TACAAAACCC AAGAGAGTGA GGTTAGTGGT GACAGATGGC 
ACAGTAGACT TAGATCTACA GTACTCGGAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC104930715 cDNA ORF clone, Larimichthys crocea(large yellow croaker)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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