kmt2e gene cDNA ORF clone, Larimichthys crocea(large yellow croaker)

mRNA	Protein	Name
XM_019278448.1	XP_019133993.1	histone-lysine N-methyltransferase 2E isoform X2
XM_019278449.1	XP_019133994.1	histone-lysine N-methyltransferase 2E isoform X4
XM_010738912.2	XP_010737214.2	histone-lysine N-methyltransferase 2E isoform X5
XM_010738912.3	XP_010737214.3	inactive histone-lysine N-methyltransferase 2E isoform X5
XM_019278450.2	XP_019133995.2	inactive histone-lysine N-methyltransferase 2E isoform X6
XM_019278447.1	XP_019133992.1	histone-lysine N-methyltransferase 2E isoform X1
XM_010738913.2	XP_010737215.2	histone-lysine N-methyltransferase 2E isoform X8
XM_010738910.3	XP_010737212.3	inactive histone-lysine N-methyltransferase 2E isoform X3
XM_027291951.1	XP_027147752.1	inactive histone-lysine N-methyltransferase 2E isoform X9
XM_019278450.1	XP_019133995.1	histone-lysine N-methyltransferase 2E isoform X6
XM_019278451.1	XP_019133996.1	histone-lysine N-methyltransferase 2E isoform X7
XM_019278448.2	XP_019133993.2	inactive histone-lysine N-methyltransferase 2E isoform X2
XM_019278449.2	XP_019133994.2	inactive histone-lysine N-methyltransferase 2E isoform X4
XM_019278447.2	XP_019133992.2	inactive histone-lysine N-methyltransferase 2E isoform X1
XM_019278451.2	XP_019133996.2	inactive histone-lysine N-methyltransferase 2E isoform X7
XM_010738910.2	XP_010737212.2	histone-lysine N-methyltransferase 2E isoform X3
XM_010738913.3	XP_010737215.3	inactive histone-lysine N-methyltransferase 2E isoform X8

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OLa211863	XM_019278447.2 Latest version!	Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa211864	XM_019278448.2 Latest version!	Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa211865	XM_010738910.3 Latest version!	Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OLa211866	XM_019278449.2 Latest version!	Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OLa211867	XM_010738912.3 Latest version!	Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OLa211868	XM_019278450.2 Latest version!	Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OLa211869	XM_019278451.2 Latest version!	Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OLa211870	XM_010738913.3 Latest version!	Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OLa211871	XM_027291951.1 Latest version!	Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OLa112873	XM_019278447.1 Latest version!	Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OLa112874	XM_019278448.1 Latest version!	Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OLa112875	XM_010738910.2 Latest version!	Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OLa112876	XM_019278449.1 Latest version!	Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OLa112877	XM_010738912.2 Latest version!	Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OLa112878	XM_019278450.1 Latest version!	Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OLa112879	XM_019278451.1 Latest version!	Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OLa112880	XM_010738913.2 Latest version!	Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00

kmt2e ( XM_019278447.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019133992.2 Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OLa211863
Clone ID Related Accession (Same CDS sequence)	XM_019278447.2
Accession Version	XM_019278447.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	inactive histone-lysine N-methyltransferase 2E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040030.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_019278447.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACAGCAGACA CCTCATACCT GGACATGGCT 
GCGGGCTCAG ACAGACCAGA ATCGGTGGAG GCCAGCCCTG TGGTGGTGGA AAAGTCCAGC 
TACCCACACC AGATCTACAG CAGCAGTTCT CACCATTCCC ACAGTTACAT TGGCCTGCCT 
TACGCTGACC ATAACTATGG GGCGCGGCCC CCACCTACAC CACCAGCCTC CCCTCCCCCC 
TCCATGTTGA TCCGACAAGG AGAGGGGGGG TTGTTTGTTC CAGGGGGCCA GGACGAAGCA 
TCAAGGGGCA CGACGCTCAG CACCTCAGAG GATGGCAGCT ACGGGGCTGA CATCACCCGC 
TGCATCTGTG GCTTCACCCA CGATGACGGC TACATGATCT GCTGTGATAA GTGCAGTGCC 
TGGCAGCATA TAGACTGCAT GGGCATCGAC AGGCAGAACA TTCCTGAGAC CTACCTGTGT 
GAACGCTGCC AACCACGACA CCTGGATCGA GAGAGGGCCA TCCTGCTGCA GACCAGGAAA 
CGGGAATGTT TGTCTGATGG GGACACGAGT GCTACAGAGA GTGGAGATGA GGTGCCGCTA 
GAGCTCTACT CCACCTTCCA ACACACGCCT ACCACCATCA CCCTGACTAC CGCGCGCCTT 
GGCAATAAGC AGGCTGATAA GAAACGCAAA AAGAGCGGTG ATAAAGAGCC TCCAGCGTCT 
TCTGCCCGAG CAAAGAAGGC CTTCCGAGAA GGGTCCAGAA AGTCCTCCAG AGTAAAGGGT 
GCAGCGCCAG AGCAGGAGCC AACAGAGTCC CTGTGGGAGA ACAAGATCAA GACGTGGATG 
GAGCGTTATG AGGAGGCCAG CAGCAATCAG TACAGCGAGG ATGTCCAGGT CCTGTTGCGT 
GTCAAAGAAC AAGGCGATGG CAAGAGCCTG GCGTACAACA CGCACCCTGC CTCTTTCAAA 
CCCCCTGTGG AGAGTCAAGT TCAGAAGAAC AAGAAGATCC TCAAAGCGGT GCGAGACTTG 
GCCCCAGACT CCCTCATCAT TGAGTACAGG GGCAAGTTCA TGCTCAGACA GCAGTTTGAG 
GCCAACGGAT ACTTCTTTAA GAGGCCATAC CCATTTGTGT TATTTTATTC TAAATTTGAC 
GGACTGGAGA TGTGCGTGGA CGCTCGTAGC TTCGGCAACG AAGCGCGCTT CATCCGTCGC 
TCCTGCACCC CTAATTCTGA AGTGCGGCAC GTCATTGAGG ACGGCATGCT GCATTTATAC 
ATCTACTCAT TGAGGCCGAT CACCAAGGGC ACAGAGATCA CCATCGGTTT TGATTTTGAC 
TATGGCAGCT GTAAATACAA GGTGGACTGT GCCTGCGTGA AGGGGAATCA GGAGTGCCCG 
GTGCTCAAGC ACAACCTCGA ACCCACAGAG AACCTGGTCG GCCGGCGCAG AGGGAGTCGT 
AAGGACAAGG AGACGGTTCG AGACGACCTG GGCCAGAACC AGAACATGGG TCTGGACGGC 
GAGGGGAAGG GCAAGAGCGT GGGCGACGGC AAACAGAGGA AGCTCTCTCC TCTTCGCCTC 
TCTATCTCAA ACAACCAGGA TCCTGAGTTA TATGAGGATC TAGAAGACAA AACCTCCGTT 
AGCAATGAAG TAGAGATGGA GTCAGAGGAG CAGATTGCAG AGAGGAGGAG GAAGATGGCC 
AACCCAGCGG AGCAGTCCCA TCTCCCTGTC GGGGCGGCTT CCAGCTGGAA AGAACTGAAA 
CACAAGGAGG TAAACTTTAC TCCACCCATC TACCAGCCTC CCCACCACCA CCTCTCCCCC 
AAATCTCCCA GAGAGACACG AGAGGAGAGG AAGATGGAGG CCATCCTGCA AGCGTTTGCC 
CGAATGGAGA AGAGAGAGAA GCGAAGGGAG CAGGCGCTGG AAAGAATCGG TAGCATCAAG 
TCGGAGGTCG GGGGACGCAG TGACATCAAG GAGGAGCCTC CAGCCACGCC AGAGACGGCA 
GATTCTCCAG CTGTTATGCA GCCGCTCCTG GAGGTGAAAG AGGAGCCAGG ATTAAAGCCG 
GCCAAGGTCA AAGGCTCGAG GAACAGGAAA AGTTTCTCAA GGAACCGCAC GCACATTGGC 
CAACAGCGCC GGAGAGCTCG CACCATCAGC ACCTGCTCTG ACCTGCAGCC TGGCTCTCCA 
ATTGAATCTG TGGAGCCTCT GAGCAACGAA GCCCCCGAAG GAGAGACACC CACCGCACCA 
GAGCCGGAGG CAATCTCTTC CCAAGCACCA GACACCAGCC CTCCGCACAG CAGCTCACCT 
GCACCTGACA GAAACCGCAC CGGGAGCAAG AACTTCAAAA CTAAAAAGCA CTTTGTGAGT 
GAGTGGGTGG GAGAGAAGCA GCAGGACCGT GGTGCAGTAC GAACCCCAGA GCCGGCCCCC 
GAGAGGCCGC TGAGAATAAG CAGTGACCCC GAAGTACTCG CCACACAGCT GAACGCCCTC 
CCAGGCATGG CCTGCTCTCC ACAGGTCTAC AGCACGCCCA AACACTACGT CCGCTTCTCG 
TCCCCTTTCC TGGCGAACCG CAGTCCGACA ACTCCCGGAG TCCCCACTGG GAGACGGCGT 
TCCAGAGAAC TGCCAGAAAC GCCGCCAACC ACCGGCTCCT GCAAGAAGCG ATGGTTGAAG 
CAGGCCCTTG AGGAAGAGGG ATCCACCAGC CCAGCCAGAC GACCAAGCCT CCTCATGCCC 
TGTGAGGGTC CTCTTAGCCC CTCAATCAAC GGAGACTCTG ACAGCCCTCT TCCCTACAAT 
GGCACCTGCT CGCTACCTGA GTTGCCCACG CCTTTGAAAA AGCGCCGGTT GAGTCCATTA 
GATGCCTGTA TGTCTGAGAG CTCCACACCC TACGGCTCCC CTTGTGCCAC GCCCACCAGG 
GCAGACCAAT CGGAGACACC CGCAACCCCC ATCTTACTGG CTACCCCACC GCGTCCCAGA 
ACGGAGGAGC CCAGTACAGA GCCCCCGCCC AACACCCCAA CGCAGACACT TAACGCCCCT 
CTGGAGAGTG AATCCTCTGT GGAGAGCTCC CCAGAGCTCA GCCGGAAACC CAGTGTACAA 
GAGGCCGATC GTCCTCCTTC GTTGGTCTCC TCTCCCTGCA TCAGGGCTCC CAGTTCAGAC 
GGACTCCCGG CTGAAGCCAA AGTGTCGGTT CCTGAGAGTC CACAGCCTCC GGTCGCCGAC 
TCTGTGGACT GTGGAGAGGA CAGGACCGAT GGTGGGGTTG TAGAAGGCAG TAGTGAGCCT 
TCCTCGTCCG CAGAAACATG TGCTTCCTCT TTTCCTGGCT GGATAAAAAG CCCAGACAGA 
GGCCCGACTG GACCAGCTGG CCTGAACTTC TCTCCAGTCA ACTCAAACCT AAGGGACCTC 
ACCCCCTCAC ACACTCTGGA GCCCCTGGCG GCTCCCTTCA GGCCCGACGC TGCAGCTGGG 
GCTGCGGCAG GGTCCACAGC AGGGACCGGG TCATTGGTTG GCCCTCAAGC CCCCTTCAGT 
GAAGGCCAGG GGCAGCTGTT TTACCCCTGC TCTGAGGAGG GAGGTTCACT CGGCTTCTCG 
CGCTCACTGA ATGGGGATGG CAGCGGTGAA GGAGGAGGAT CAGCGCAGAA CCCCCCACAG 
AAGAAAAAGG TTTCCCTGCT AGAATACAGG AAACGTCAGC GCGAAGCCCG TCGCAGCGGC 
TCCAAGACCG AGTGCAGCTC TCCTGTCTCC ACAGTGCCAC CTCTGACCGT GGACGCCTTC 
CCCGTCGCGC TGGAGACGAC AAGCGAACCT CCACTACCCC CTGCTCCTAC AGCTGTCTGC 
AACGCCAACA ACACCATCGT TGTTAAAGAG CCCCAGACGA GCGAGGAGGC CGAGACGCCC 
GGAGAGAAAG GCGAGAAGGA AGGAGGAGAA GGACAGTGGA CGTCGTCCAC TTCTGTAGAG 
CAGGCCCGAG AACGCAGCTA CCACAGAGCT CTGCTGCTCA GCAAAGACAA GGACACGGAT 
GGTGAGACAG AAGGTGGAGA TACACCTGCA CTGAGAGATT GCCCGTCTCC AAGTCTTCAG 
AAGACCCCGA CTCACACCCC CTGCTCTCCT GGTCCTCTCG CTCAGTCCAC CAATCGGCCA 
CCGAAGGAGG AAGACGGCGA CGGGCAGCCG AGCAAGCCTG CGGGACCCAA GTCGGCTCCT 
CTGACCCCCA CAAAGCTGCA TCCCACCCCG TTACCCTCCT CGCCGGTCCA CTATCCGGGA 
CCCTCCCTCC TGCACTCGCC CAAGCCTCAG CCTCAGGGCT CGCCGTACCG CAGCCAGAGG 
GCGCTGTTCT CCGCTCAGCC TCAAAACCAA CCGCAGCCCC AGGCTCAGAC TGGCCCAGCT 
CCTTTCCCCC AGTATAACAC ACAGAGTGCT CCACCGCCAC CTCCCCCTCC TCCACCAGCA 
CCACCGGCCT CCACGGCGTA TTTTCCCAGC CAGAGCCCCT CGCCTGCTGG ACCTTTCCCC 
GGGTTCAAAC CCGCTGTCAC ACCCCCTTAC CCTCCGTGTT CTCAGCCCCT GATGCAGACT 
CTTCCCCACA GCGTGCACTA TCAGAGCTCA GCCGCTCCAC CGCCGCCGCC TCCTCCGCCC 
CCGCACCCGA TGCCCGGCCC CACCCTGCTG CACGTCAACC TGCAGCCCCC TCCCATCCAG 
CAGCACCAGC TCATGCTGAC CACCGCCCCT CCGCCGCCTC CTCCCCCACA GGGCCAGACC 
TCACAGCAGC AGCAGCAGCA GCCTCCCGCT GGCAGCTCCC TGCTGTCGCT CACCCCTCCT 
CCGCCGCCTC CTCCACCTCC TCCAGCCCCC TCGACCAACG CCCCGATGCA GGCCCACCAC 
TTTCAGAACT TAGGGGGTTT TCAGCCGGCG TTGCTACACC CGGGCGGCGC CGCCACAAAC 
CCTTCAGTGC CCCAATCCAC GTACCCCCCA CCCCTTCAGC AGACCGGACT GCCTCCACCT 
CCCCCTCCTC CTCCCCAACA GACTCAACAA GGCCAGGCTC AGGCCGCCGC CTCCCAGATG 
CCTTCTGGGA CTCGTGGAGC TCCTGCGTCC TCGACCCCCT TCCACAGCTC GGGGTACCTG 
AGCACAGGGT GGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019133992.2
CDS	484..5541
Translation	MSIVIPVGVDTADTSYLDMAAGSDRPESVEASPVVVEKSSYPHQIYSSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPSMLIRQGEGGLFVPGGQDEASRGTTLSTSEDGSYGADITRCICGFTHDDGYMICCDKCSAWQHIDCMGIDRQNIPETYLCERCQPRHLDRERAILLQTRKRECLSDGDTSATESGDEVPLELYSTFQHTPTTITLTTARLGNKQADKKRKKSGDKEPPASSARAKKAFREGSRKSSRVKGAAPEQEPTESLWENKIKTWMERYEEASSNQYSEDVQVLLRVKEQGDGKSLAYNTHPASFKPPVESQVQKNKKILKAVRDLAPDSLIIEYRGKFMLRQQFEANGYFFKRPYPFVLFYSKFDGLEMCVDARSFGNEARFIRRSCTPNSEVRHVIEDGMLHLYIYSLRPITKGTEITIGFDFDYGSCKYKVDCACVKGNQECPVLKHNLEPTENLVGRRRGSRKDKETVRDDLGQNQNMGLDGEGKGKSVGDGKQRKLSPLRLSISNNQDPELYEDLEDKTSVSNEVEMESEEQIAERRRKMANPAEQSHLPVGAASSWKELKHKEVNFTPPIYQPPHHHLSPKSPRETREERKMEAILQAFARMEKREKRREQALERIGSIKSEVGGRSDIKEEPPATPETADSPAVMQPLLEVKEEPGLKPAKVKGSRNRKSFSRNRTHIGQQRRRARTISTCSDLQPGSPIESVEPLSNEAPEGETPTAPEPEAISSQAPDTSPPHSSSPAPDRNRTGSKNFKTKKHFVSEWVGEKQQDRGAVRTPEPAPERPLRISSDPEVLATQLNALPGMACSPQVYSTPKHYVRFSSPFLANRSPTTPGVPTGRRRSRELPETPPTTGSCKKRWLKQALEEEGSTSPARRPSLLMPCEGPLSPSINGDSDSPLPYNGTCSLPELPTPLKKRRLSPLDACMSESSTPYGSPCATPTRADQSETPATPILLATPPRPRTEEPSTEPPPNTPTQTLNAPLESESSVESSPELSRKPSVQEADRPPSLVSSPCIRAPSSDGLPAEAKVSVPESPQPPVADSVDCGEDRTDGGVVEGSSEPSSSAETCASSFPGWIKSPDRGPTGPAGLNFSPVNSNLRDLTPSHTLEPLAAPFRPDAAAGAAAGSTAGTGSLVGPQAPFSEGQGQLFYPCSEEGGSLGFSRSLNGDGSGEGGGSAQNPPQKKKVSLLEYRKRQREARRSGSKTECSSPVSTVPPLTVDAFPVALETTSEPPLPPAPTAVCNANNTIVVKEPQTSEEAETPGEKGEKEGGEGQWTSSTSVEQARERSYHRALLLSKDKDTDGETEGGDTPALRDCPSPSLQKTPTHTPCSPGPLAQSTNRPPKEEDGDGQPSKPAGPKSAPLTPTKLHPTPLPSSPVHYPGPSLLHSPKPQPQGSPYRSQRALFSAQPQNQPQPQAQTGPAPFPQYNTQSAPPPPPPPPPAPPASTAYFPSQSPSPAGPFPGFKPAVTPPYPPCSQPLMQTLPHSVHYQSSAAPPPPPPPPPHPMPGPTLLHVNLQPPPIQQHQLMLTTAPPPPPPPQGQTSQQQQQQPPAGSSLLSLTPPPPPPPPPPAPSTNAPMQAHHFQNLGGFQPALLHPGGAATNPSVPQSTYPPPLQQTGLPPPPPPPPQQTQQGQAQAAASQMPSGTRGAPASSTPFHSSGYLSTGWH

Target ORF information:

RefSeq Version	XM_019278447.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019278447.2

ORF Insert Sequence:

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACAGCAGACA CCTCATACCT GGACATGGCT 
GCGGGCTCAG ACAGACCAGA ATCGGTGGAG GCCAGCCCTG TGGTGGTGGA AAAGTCCAGC 
TACCCACACC AGATCTACAG CAGCAGTTCT CACCATTCCC ACAGTTACAT TGGCCTGCCT 
TACGCTGACC ATAACTATGG GGCGCGGCCC CCACCTACAC CACCAGCCTC CCCTCCCCCC 
TCCATGTTGA TCCGACAAGG AGAGGGGGGG TTGTTTGTTC CAGGGGGCCA GGACGAAGCA 
TCAAGGGGCA CGACGCTCAG CACCTCAGAG GATGGCAGCT ACGGGGCTGA CATCACCCGC 
TGCATCTGTG GCTTCACCCA CGATGACGGC TACATGATCT GCTGTGATAA GTGCAGTGCC 
TGGCAGCATA TAGACTGCAT GGGCATCGAC AGGCAGAACA TTCCTGAGAC CTACCTGTGT 
GAACGCTGCC AACCACGACA CCTGGATCGA GAGAGGGCCA TCCTGCTGCA GACCAGGAAA 
CGGGAATGTT TGTCTGATGG GGACACGAGT GCTACAGAGA GTGGAGATGA GGTGCCGCTA 
GAGCTCTACT CCACCTTCCA ACACACGCCT ACCACCATCA CCCTGACTAC CGCGCGCCTT 
GGCAATAAGC AGGCTGATAA GAAACGCAAA AAGAGCGGTG ATAAAGAGCC TCCAGCGTCT 
TCTGCCCGAG CAAAGAAGGC CTTCCGAGAA GGGTCCAGAA AGTCCTCCAG AGTAAAGGGT 
GCAGCGCCAG AGCAGGAGCC AACAGAGTCC CTGTGGGAGA ACAAGATCAA GACGTGGATG 
GAGCGTTATG AGGAGGCCAG CAGCAATCAG TACAGCGAGG ATGTCCAGGT CCTGTTGCGT 
GTCAAAGAAC AAGGCGATGG CAAGAGCCTG GCGTACAACA CGCACCCTGC CTCTTTCAAA 
CCCCCTGTGG AGAGTCAAGT TCAGAAGAAC AAGAAGATCC TCAAAGCGGT GCGAGACTTG 
GCCCCAGACT CCCTCATCAT TGAGTACAGG GGCAAGTTCA TGCTCAGACA GCAGTTTGAG 
GCCAACGGAT ACTTCTTTAA GAGGCCATAC CCATTTGTGT TATTTTATTC TAAATTTGAC 
GGACTGGAGA TGTGCGTGGA CGCTCGTAGC TTCGGCAACG AAGCGCGCTT CATCCGTCGC 
TCCTGCACCC CTAATTCTGA AGTGCGGCAC GTCATTGAGG ACGGCATGCT GCATTTATAC 
ATCTACTCAT TGAGGCCGAT CACCAAGGGC ACAGAGATCA CCATCGGTTT TGATTTTGAC 
TATGGCAGCT GTAAATACAA GGTGGACTGT GCCTGCGTGA AGGGGAATCA GGAGTGCCCG 
GTGCTCAAGC ACAACCTCGA ACCCACAGAG AACCTGGTCG GCCGGCGCAG AGGGAGTCGT 
AAGGACAAGG AGACGGTTCG AGACGACCTG GGCCAGAACC AGAACATGGG TCTGGACGGC 
GAGGGGAAGG GCAAGAGCGT GGGCGACGGC AAACAGAGGA AGCTCTCTCC TCTTCGCCTC 
TCTATCTCAA ACAACCAGGA TCCTGAGTTA TATGAGGATC TAGAAGACAA AACCTCCGTT 
AGCAATGAAG TAGAGATGGA GTCAGAGGAG CAGATTGCAG AGAGGAGGAG GAAGATGGCC 
AACCCAGCGG AGCAGTCCCA TCTCCCTGTC GGGGCGGCTT CCAGCTGGAA AGAACTGAAA 
CACAAGGAGG TAAACTTTAC TCCACCCATC TACCAGCCTC CCCACCACCA CCTCTCCCCC 
AAATCTCCCA GAGAGACACG AGAGGAGAGG AAGATGGAGG CCATCCTGCA AGCGTTTGCC 
CGAATGGAGA AGAGAGAGAA GCGAAGGGAG CAGGCGCTGG AAAGAATCGG TAGCATCAAG 
TCGGAGGTCG GGGGACGCAG TGACATCAAG GAGGAGCCTC CAGCCACGCC AGAGACGGCA 
GATTCTCCAG CTGTTATGCA GCCGCTCCTG GAGGTGAAAG AGGAGCCAGG ATTAAAGCCG 
GCCAAGGTCA AAGGCTCGAG GAACAGGAAA AGTTTCTCAA GGAACCGCAC GCACATTGGC 
CAACAGCGCC GGAGAGCTCG CACCATCAGC ACCTGCTCTG ACCTGCAGCC TGGCTCTCCA 
ATTGAATCTG TGGAGCCTCT GAGCAACGAA GCCCCCGAAG GAGAGACACC CACCGCACCA 
GAGCCGGAGG CAATCTCTTC CCAAGCACCA GACACCAGCC CTCCGCACAG CAGCTCACCT 
GCACCTGACA GAAACCGCAC CGGGAGCAAG AACTTCAAAA CTAAAAAGCA CTTTGTGAGT 
GAGTGGGTGG GAGAGAAGCA GCAGGACCGT GGTGCAGTAC GAACCCCAGA GCCGGCCCCC 
GAGAGGCCGC TGAGAATAAG CAGTGACCCC GAAGTACTCG CCACACAGCT GAACGCCCTC 
CCAGGCATGG CCTGCTCTCC ACAGGTCTAC AGCACGCCCA AACACTACGT CCGCTTCTCG 
TCCCCTTTCC TGGCGAACCG CAGTCCGACA ACTCCCGGAG TCCCCACTGG GAGACGGCGT 
TCCAGAGAAC TGCCAGAAAC GCCGCCAACC ACCGGCTCCT GCAAGAAGCG ATGGTTGAAG 
CAGGCCCTTG AGGAAGAGGG ATCCACCAGC CCAGCCAGAC GACCAAGCCT CCTCATGCCC 
TGTGAGGGTC CTCTTAGCCC CTCAATCAAC GGAGACTCTG ACAGCCCTCT TCCCTACAAT 
GGCACCTGCT CGCTACCTGA GTTGCCCACG CCTTTGAAAA AGCGCCGGTT GAGTCCATTA 
GATGCCTGTA TGTCTGAGAG CTCCACACCC TACGGCTCCC CTTGTGCCAC GCCCACCAGG 
GCAGACCAAT CGGAGACACC CGCAACCCCC ATCTTACTGG CTACCCCACC GCGTCCCAGA 
ACGGAGGAGC CCAGTACAGA GCCCCCGCCC AACACCCCAA CGCAGACACT TAACGCCCCT 
CTGGAGAGTG AATCCTCTGT GGAGAGCTCC CCAGAGCTCA GCCGGAAACC CAGTGTACAA 
GAGGCCGATC GTCCTCCTTC GTTGGTCTCC TCTCCCTGCA TCAGGGCTCC CAGTTCAGAC 
GGACTCCCGG CTGAAGCCAA AGTGTCGGTT CCTGAGAGTC CACAGCCTCC GGTCGCCGAC 
TCTGTGGACT GTGGAGAGGA CAGGACCGAT GGTGGGGTTG TAGAAGGCAG TAGTGAGCCT 
TCCTCGTCCG CAGAAACATG TGCTTCCTCT TTTCCTGGCT GGATAAAAAG CCCAGACAGA 
GGCCCGACTG GACCAGCTGG CCTGAACTTC TCTCCAGTCA ACTCAAACCT AAGGGACCTC 
ACCCCCTCAC ACACTCTGGA GCCCCTGGCG GCTCCCTTCA GGCCCGACGC TGCAGCTGGG 
GCTGCGGCAG GGTCCACAGC AGGGACCGGG TCATTGGTTG GCCCTCAAGC CCCCTTCAGT 
GAAGGCCAGG GGCAGCTGTT TTACCCCTGC TCTGAGGAGG GAGGTTCACT CGGCTTCTCG 
CGCTCACTGA ATGGGGATGG CAGCGGTGAA GGAGGAGGAT CAGCGCAGAA CCCCCCACAG 
AAGAAAAAGG TTTCCCTGCT AGAATACAGG AAACGTCAGC GCGAAGCCCG TCGCAGCGGC 
TCCAAGACCG AGTGCAGCTC TCCTGTCTCC ACAGTGCCAC CTCTGACCGT GGACGCCTTC 
CCCGTCGCGC TGGAGACGAC AAGCGAACCT CCACTACCCC CTGCTCCTAC AGCTGTCTGC 
AACGCCAACA ACACCATCGT TGTTAAAGAG CCCCAGACGA GCGAGGAGGC CGAGACGCCC 
GGAGAGAAAG GCGAGAAGGA AGGAGGAGAA GGACAGTGGA CGTCGTCCAC TTCTGTAGAG 
CAGGCCCGAG AACGCAGCTA CCACAGAGCT CTGCTGCTCA GCAAAGACAA GGACACGGAT 
GGTGAGACAG AAGGTGGAGA TACACCTGCA CTGAGAGATT GCCCGTCTCC AAGTCTTCAG 
AAGACCCCGA CTCACACCCC CTGCTCTCCT GGTCCTCTCG CTCAGTCCAC CAATCGGCCA 
CCGAAGGAGG AAGACGGCGA CGGGCAGCCG AGCAAGCCTG CGGGACCCAA GTCGGCTCCT 
CTGACCCCCA CAAAGCTGCA TCCCACCCCG TTACCCTCCT CGCCGGTCCA CTATCCGGGA 
CCCTCCCTCC TGCACTCGCC CAAGCCTCAG CCTCAGGGCT CGCCGTACCG CAGCCAGAGG 
GCGCTGTTCT CCGCTCAGCC TCAAAACCAA CCGCAGCCCC AGGCTCAGAC TGGCCCAGCT 
CCTTTCCCCC AGTATAACAC ACAGAGTGCT CCACCGCCAC CTCCCCCTCC TCCACCAGCA 
CCACCGGCCT CCACGGCGTA TTTTCCCAGC CAGAGCCCCT CGCCTGCTGG ACCTTTCCCC 
GGGTTCAAAC CCGCTGTCAC ACCCCCTTAC CCTCCGTGTT CTCAGCCCCT GATGCAGACT 
CTTCCCCACA GCGTGCACTA TCAGAGCTCA GCCGCTCCAC CGCCGCCGCC TCCTCCGCCC 
CCGCACCCGA TGCCCGGCCC CACCCTGCTG CACGTCAACC TGCAGCCCCC TCCCATCCAG 
CAGCACCAGC TCATGCTGAC CACCGCCCCT CCGCCGCCTC CTCCCCCACA GGGCCAGACC 
TCACAGCAGC AGCAGCAGCA GCCTCCCGCT GGCAGCTCCC TGCTGTCGCT CACCCCTCCT 
CCGCCGCCTC CTCCACCTCC TCCAGCCCCC TCGACCAACG CCCCGATGCA GGCCCACCAC 
TTTCAGAACT TAGGGGGTTT TCAGCCGGCG TTGCTACACC CGGGCGGCGC CGCCACAAAC 
CCTTCAGTGC CCCAATCCAC GTACCCCCCA CCCCTTCAGC AGACCGGACT GCCTCCACCT 
CCCCCTCCTC CTCCCCAACA GACTCAACAA GGCCAGGCTC AGGCCGCCGC CTCCCAGATG 
CCTTCTGGGA CTCGTGGAGC TCCTGCGTCC TCGACCCCCT TCCACAGCTC GGGGTACCTG 
AGCACAGGGT GGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kmt2e ( XM_019278448.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019133993.2 Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X2, mRNA.

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CloneID	OLa211864
Clone ID Related Accession (Same CDS sequence)	XM_019278448.2
Accession Version	XM_019278448.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	inactive histone-lysine N-methyltransferase 2E isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040030.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_019278448.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACAGCAGACA CCTCATACCT GGACATGGCT 
GCGGGCTCAG AACCAGAATC GGTGGAGGCC AGCCCTGTGG TGGTGGAAAA GTCCAGCTAC 
CCACACCAGA TCTACAGCAG CAGTTCTCAC CATTCCCACA GTTACATTGG CCTGCCTTAC 
GCTGACCATA ACTATGGGGC GCGGCCCCCA CCTACACCAC CAGCCTCCCC TCCCCCCTCC 
ATGTTGATCC GACAAGGAGA GGGGGGGTTG TTTGTTCCAG GGGGCCAGGA CGAAGCATCA 
AGGGGCACGA CGCTCAGCAC CTCAGAGGAT GGCAGCTACG GGGCTGACAT CACCCGCTGC 
ATCTGTGGCT TCACCCACGA TGACGGCTAC ATGATCTGCT GTGATAAGTG CAGTGCCTGG 
CAGCATATAG ACTGCATGGG CATCGACAGG CAGAACATTC CTGAGACCTA CCTGTGTGAA 
CGCTGCCAAC CACGACACCT GGATCGAGAG AGGGCCATCC TGCTGCAGAC CAGGAAACGG 
GAATGTTTGT CTGATGGGGA CACGAGTGCT ACAGAGAGTG GAGATGAGGT GCCGCTAGAG 
CTCTACTCCA CCTTCCAACA CACGCCTACC ACCATCACCC TGACTACCGC GCGCCTTGGC 
AATAAGCAGG CTGATAAGAA ACGCAAAAAG AGCGGTGATA AAGAGCCTCC AGCGTCTTCT 
GCCCGAGCAA AGAAGGCCTT CCGAGAAGGG TCCAGAAAGT CCTCCAGAGT AAAGGGTGCA 
GCGCCAGAGC AGGAGCCAAC AGAGTCCCTG TGGGAGAACA AGATCAAGAC GTGGATGGAG 
CGTTATGAGG AGGCCAGCAG CAATCAGTAC AGCGAGGATG TCCAGGTCCT GTTGCGTGTC 
AAAGAACAAG GCGATGGCAA GAGCCTGGCG TACAACACGC ACCCTGCCTC TTTCAAACCC 
CCTGTGGAGA GTCAAGTTCA GAAGAACAAG AAGATCCTCA AAGCGGTGCG AGACTTGGCC 
CCAGACTCCC TCATCATTGA GTACAGGGGC AAGTTCATGC TCAGACAGCA GTTTGAGGCC 
AACGGATACT TCTTTAAGAG GCCATACCCA TTTGTGTTAT TTTATTCTAA ATTTGACGGA 
CTGGAGATGT GCGTGGACGC TCGTAGCTTC GGCAACGAAG CGCGCTTCAT CCGTCGCTCC 
TGCACCCCTA ATTCTGAAGT GCGGCACGTC ATTGAGGACG GCATGCTGCA TTTATACATC 
TACTCATTGA GGCCGATCAC CAAGGGCACA GAGATCACCA TCGGTTTTGA TTTTGACTAT 
GGCAGCTGTA AATACAAGGT GGACTGTGCC TGCGTGAAGG GGAATCAGGA GTGCCCGGTG 
CTCAAGCACA ACCTCGAACC CACAGAGAAC CTGGTCGGCC GGCGCAGAGG GAGTCGTAAG 
GACAAGGAGA CGGTTCGAGA CGACCTGGGC CAGAACCAGA ACATGGGTCT GGACGGCGAG 
GGGAAGGGCA AGAGCGTGGG CGACGGCAAA CAGAGGAAGC TCTCTCCTCT TCGCCTCTCT 
ATCTCAAACA ACCAGGATCC TGAGTTATAT GAGGATCTAG AAGACAAAAC CTCCGTTAGC 
AATGAAGTAG AGATGGAGTC AGAGGAGCAG ATTGCAGAGA GGAGGAGGAA GATGGCCAAC 
CCAGCGGAGC AGTCCCATCT CCCTGTCGGG GCGGCTTCCA GCTGGAAAGA ACTGAAACAC 
AAGGAGGTAA ACTTTACTCC ACCCATCTAC CAGCCTCCCC ACCACCACCT CTCCCCCAAA 
TCTCCCAGAG AGACACGAGA GGAGAGGAAG ATGGAGGCCA TCCTGCAAGC GTTTGCCCGA 
ATGGAGAAGA GAGAGAAGCG AAGGGAGCAG GCGCTGGAAA GAATCGGTAG CATCAAGTCG 
GAGGTCGGGG GACGCAGTGA CATCAAGGAG GAGCCTCCAG CCACGCCAGA GACGGCAGAT 
TCTCCAGCTG TTATGCAGCC GCTCCTGGAG GTGAAAGAGG AGCCAGGATT AAAGCCGGCC 
AAGGTCAAAG GCTCGAGGAA CAGGAAAAGT TTCTCAAGGA ACCGCACGCA CATTGGCCAA 
CAGCGCCGGA GAGCTCGCAC CATCAGCACC TGCTCTGACC TGCAGCCTGG CTCTCCAATT 
GAATCTGTGG AGCCTCTGAG CAACGAAGCC CCCGAAGGAG AGACACCCAC CGCACCAGAG 
CCGGAGGCAA TCTCTTCCCA AGCACCAGAC ACCAGCCCTC CGCACAGCAG CTCACCTGCA 
CCTGACAGAA ACCGCACCGG GAGCAAGAAC TTCAAAACTA AAAAGCACTT TGTGAGTGAG 
TGGGTGGGAG AGAAGCAGCA GGACCGTGGT GCAGTACGAA CCCCAGAGCC GGCCCCCGAG 
AGGCCGCTGA GAATAAGCAG TGACCCCGAA GTACTCGCCA CACAGCTGAA CGCCCTCCCA 
GGCATGGCCT GCTCTCCACA GGTCTACAGC ACGCCCAAAC ACTACGTCCG CTTCTCGTCC 
CCTTTCCTGG CGAACCGCAG TCCGACAACT CCCGGAGTCC CCACTGGGAG ACGGCGTTCC 
AGAGAACTGC CAGAAACGCC GCCAACCACC GGCTCCTGCA AGAAGCGATG GTTGAAGCAG 
GCCCTTGAGG AAGAGGGATC CACCAGCCCA GCCAGACGAC CAAGCCTCCT CATGCCCTGT 
GAGGGTCCTC TTAGCCCCTC AATCAACGGA GACTCTGACA GCCCTCTTCC CTACAATGGC 
ACCTGCTCGC TACCTGAGTT GCCCACGCCT TTGAAAAAGC GCCGGTTGAG TCCATTAGAT 
GCCTGTATGT CTGAGAGCTC CACACCCTAC GGCTCCCCTT GTGCCACGCC CACCAGGGCA 
GACCAATCGG AGACACCCGC AACCCCCATC TTACTGGCTA CCCCACCGCG TCCCAGAACG 
GAGGAGCCCA GTACAGAGCC CCCGCCCAAC ACCCCAACGC AGACACTTAA CGCCCCTCTG 
GAGAGTGAAT CCTCTGTGGA GAGCTCCCCA GAGCTCAGCC GGAAACCCAG TGTACAAGAG 
GCCGATCGTC CTCCTTCGTT GGTCTCCTCT CCCTGCATCA GGGCTCCCAG TTCAGACGGA 
CTCCCGGCTG AAGCCAAAGT GTCGGTTCCT GAGAGTCCAC AGCCTCCGGT CGCCGACTCT 
GTGGACTGTG GAGAGGACAG GACCGATGGT GGGGTTGTAG AAGGCAGTAG TGAGCCTTCC 
TCGTCCGCAG AAACATGTGC TTCCTCTTTT CCTGGCTGGA TAAAAAGCCC AGACAGAGGC 
CCGACTGGAC CAGCTGGCCT GAACTTCTCT CCAGTCAACT CAAACCTAAG GGACCTCACC 
CCCTCACACA CTCTGGAGCC CCTGGCGGCT CCCTTCAGGC CCGACGCTGC AGCTGGGGCT 
GCGGCAGGGT CCACAGCAGG GACCGGGTCA TTGGTTGGCC CTCAAGCCCC CTTCAGTGAA 
GGCCAGGGGC AGCTGTTTTA CCCCTGCTCT GAGGAGGGAG GTTCACTCGG CTTCTCGCGC 
TCACTGAATG GGGATGGCAG CGGTGAAGGA GGAGGATCAG CGCAGAACCC CCCACAGAAG 
AAAAAGGTTT CCCTGCTAGA ATACAGGAAA CGTCAGCGCG AAGCCCGTCG CAGCGGCTCC 
AAGACCGAGT GCAGCTCTCC TGTCTCCACA GTGCCACCTC TGACCGTGGA CGCCTTCCCC 
GTCGCGCTGG AGACGACAAG CGAACCTCCA CTACCCCCTG CTCCTACAGC TGTCTGCAAC 
GCCAACAACA CCATCGTTGT TAAAGAGCCC CAGACGAGCG AGGAGGCCGA GACGCCCGGA 
GAGAAAGGCG AGAAGGAAGG AGGAGAAGGA CAGTGGACGT CGTCCACTTC TGTAGAGCAG 
GCCCGAGAAC GCAGCTACCA CAGAGCTCTG CTGCTCAGCA AAGACAAGGA CACGGATGGT 
GAGACAGAAG GTGGAGATAC ACCTGCACTG AGAGATTGCC CGTCTCCAAG TCTTCAGAAG 
ACCCCGACTC ACACCCCCTG CTCTCCTGGT CCTCTCGCTC AGTCCACCAA TCGGCCACCG 
AAGGAGGAAG ACGGCGACGG GCAGCCGAGC AAGCCTGCGG GACCCAAGTC GGCTCCTCTG 
ACCCCCACAA AGCTGCATCC CACCCCGTTA CCCTCCTCGC CGGTCCACTA TCCGGGACCC 
TCCCTCCTGC ACTCGCCCAA GCCTCAGCCT CAGGGCTCGC CGTACCGCAG CCAGAGGGCG 
CTGTTCTCCG CTCAGCCTCA AAACCAACCG CAGCCCCAGG CTCAGACTGG CCCAGCTCCT 
TTCCCCCAGT ATAACACACA GAGTGCTCCA CCGCCACCTC CCCCTCCTCC ACCAGCACCA 
CCGGCCTCCA CGGCGTATTT TCCCAGCCAG AGCCCCTCGC CTGCTGGACC TTTCCCCGGG 
TTCAAACCCG CTGTCACACC CCCTTACCCT CCGTGTTCTC AGCCCCTGAT GCAGACTCTT 
CCCCACAGCG TGCACTATCA GAGCTCAGCC GCTCCACCGC CGCCGCCTCC TCCGCCCCCG 
CACCCGATGC CCGGCCCCAC CCTGCTGCAC GTCAACCTGC AGCCCCCTCC CATCCAGCAG 
CACCAGCTCA TGCTGACCAC CGCCCCTCCG CCGCCTCCTC CCCCACAGGG CCAGACCTCA 
CAGCAGCAGC AGCAGCAGCC TCCCGCTGGC AGCTCCCTGC TGTCGCTCAC CCCTCCTCCG 
CCGCCTCCTC CACCTCCTCC AGCCCCCTCG ACCAACGCCC CGATGCAGGC CCACCACTTT 
CAGAACTTAG GGGGTTTTCA GCCGGCGTTG CTACACCCGG GCGGCGCCGC CACAAACCCT 
TCAGTGCCCC AATCCACGTA CCCCCCACCC CTTCAGCAGA CCGGACTGCC TCCACCTCCC 
CCTCCTCCTC CCCAACAGAC TCAACAAGGC CAGGCTCAGG CCGCCGCCTC CCAGATGCCT 
TCTGGGACTC GTGGAGCTCC TGCGTCCTCG ACCCCCTTCC ACAGCTCGGG GTACCTGAGC 
ACAGGGTGGC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019133993.2
CDS	484..5538
Translation	MSIVIPVGVDTADTSYLDMAAGSEPESVEASPVVVEKSSYPHQIYSSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPSMLIRQGEGGLFVPGGQDEASRGTTLSTSEDGSYGADITRCICGFTHDDGYMICCDKCSAWQHIDCMGIDRQNIPETYLCERCQPRHLDRERAILLQTRKRECLSDGDTSATESGDEVPLELYSTFQHTPTTITLTTARLGNKQADKKRKKSGDKEPPASSARAKKAFREGSRKSSRVKGAAPEQEPTESLWENKIKTWMERYEEASSNQYSEDVQVLLRVKEQGDGKSLAYNTHPASFKPPVESQVQKNKKILKAVRDLAPDSLIIEYRGKFMLRQQFEANGYFFKRPYPFVLFYSKFDGLEMCVDARSFGNEARFIRRSCTPNSEVRHVIEDGMLHLYIYSLRPITKGTEITIGFDFDYGSCKYKVDCACVKGNQECPVLKHNLEPTENLVGRRRGSRKDKETVRDDLGQNQNMGLDGEGKGKSVGDGKQRKLSPLRLSISNNQDPELYEDLEDKTSVSNEVEMESEEQIAERRRKMANPAEQSHLPVGAASSWKELKHKEVNFTPPIYQPPHHHLSPKSPRETREERKMEAILQAFARMEKREKRREQALERIGSIKSEVGGRSDIKEEPPATPETADSPAVMQPLLEVKEEPGLKPAKVKGSRNRKSFSRNRTHIGQQRRRARTISTCSDLQPGSPIESVEPLSNEAPEGETPTAPEPEAISSQAPDTSPPHSSSPAPDRNRTGSKNFKTKKHFVSEWVGEKQQDRGAVRTPEPAPERPLRISSDPEVLATQLNALPGMACSPQVYSTPKHYVRFSSPFLANRSPTTPGVPTGRRRSRELPETPPTTGSCKKRWLKQALEEEGSTSPARRPSLLMPCEGPLSPSINGDSDSPLPYNGTCSLPELPTPLKKRRLSPLDACMSESSTPYGSPCATPTRADQSETPATPILLATPPRPRTEEPSTEPPPNTPTQTLNAPLESESSVESSPELSRKPSVQEADRPPSLVSSPCIRAPSSDGLPAEAKVSVPESPQPPVADSVDCGEDRTDGGVVEGSSEPSSSAETCASSFPGWIKSPDRGPTGPAGLNFSPVNSNLRDLTPSHTLEPLAAPFRPDAAAGAAAGSTAGTGSLVGPQAPFSEGQGQLFYPCSEEGGSLGFSRSLNGDGSGEGGGSAQNPPQKKKVSLLEYRKRQREARRSGSKTECSSPVSTVPPLTVDAFPVALETTSEPPLPPAPTAVCNANNTIVVKEPQTSEEAETPGEKGEKEGGEGQWTSSTSVEQARERSYHRALLLSKDKDTDGETEGGDTPALRDCPSPSLQKTPTHTPCSPGPLAQSTNRPPKEEDGDGQPSKPAGPKSAPLTPTKLHPTPLPSSPVHYPGPSLLHSPKPQPQGSPYRSQRALFSAQPQNQPQPQAQTGPAPFPQYNTQSAPPPPPPPPPAPPASTAYFPSQSPSPAGPFPGFKPAVTPPYPPCSQPLMQTLPHSVHYQSSAAPPPPPPPPPHPMPGPTLLHVNLQPPPIQQHQLMLTTAPPPPPPPQGQTSQQQQQQPPAGSSLLSLTPPPPPPPPPPAPSTNAPMQAHHFQNLGGFQPALLHPGGAATNPSVPQSTYPPPLQQTGLPPPPPPPPQQTQQGQAQAAASQMPSGTRGAPASSTPFHSSGYLSTGWH

Target ORF information:

RefSeq Version	XM_019278448.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019278448.2

ORF Insert Sequence:

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACAGCAGACA CCTCATACCT GGACATGGCT 
GCGGGCTCAG AACCAGAATC GGTGGAGGCC AGCCCTGTGG TGGTGGAAAA GTCCAGCTAC 
CCACACCAGA TCTACAGCAG CAGTTCTCAC CATTCCCACA GTTACATTGG CCTGCCTTAC 
GCTGACCATA ACTATGGGGC GCGGCCCCCA CCTACACCAC CAGCCTCCCC TCCCCCCTCC 
ATGTTGATCC GACAAGGAGA GGGGGGGTTG TTTGTTCCAG GGGGCCAGGA CGAAGCATCA 
AGGGGCACGA CGCTCAGCAC CTCAGAGGAT GGCAGCTACG GGGCTGACAT CACCCGCTGC 
ATCTGTGGCT TCACCCACGA TGACGGCTAC ATGATCTGCT GTGATAAGTG CAGTGCCTGG 
CAGCATATAG ACTGCATGGG CATCGACAGG CAGAACATTC CTGAGACCTA CCTGTGTGAA 
CGCTGCCAAC CACGACACCT GGATCGAGAG AGGGCCATCC TGCTGCAGAC CAGGAAACGG 
GAATGTTTGT CTGATGGGGA CACGAGTGCT ACAGAGAGTG GAGATGAGGT GCCGCTAGAG 
CTCTACTCCA CCTTCCAACA CACGCCTACC ACCATCACCC TGACTACCGC GCGCCTTGGC 
AATAAGCAGG CTGATAAGAA ACGCAAAAAG AGCGGTGATA AAGAGCCTCC AGCGTCTTCT 
GCCCGAGCAA AGAAGGCCTT CCGAGAAGGG TCCAGAAAGT CCTCCAGAGT AAAGGGTGCA 
GCGCCAGAGC AGGAGCCAAC AGAGTCCCTG TGGGAGAACA AGATCAAGAC GTGGATGGAG 
CGTTATGAGG AGGCCAGCAG CAATCAGTAC AGCGAGGATG TCCAGGTCCT GTTGCGTGTC 
AAAGAACAAG GCGATGGCAA GAGCCTGGCG TACAACACGC ACCCTGCCTC TTTCAAACCC 
CCTGTGGAGA GTCAAGTTCA GAAGAACAAG AAGATCCTCA AAGCGGTGCG AGACTTGGCC 
CCAGACTCCC TCATCATTGA GTACAGGGGC AAGTTCATGC TCAGACAGCA GTTTGAGGCC 
AACGGATACT TCTTTAAGAG GCCATACCCA TTTGTGTTAT TTTATTCTAA ATTTGACGGA 
CTGGAGATGT GCGTGGACGC TCGTAGCTTC GGCAACGAAG CGCGCTTCAT CCGTCGCTCC 
TGCACCCCTA ATTCTGAAGT GCGGCACGTC ATTGAGGACG GCATGCTGCA TTTATACATC 
TACTCATTGA GGCCGATCAC CAAGGGCACA GAGATCACCA TCGGTTTTGA TTTTGACTAT 
GGCAGCTGTA AATACAAGGT GGACTGTGCC TGCGTGAAGG GGAATCAGGA GTGCCCGGTG 
CTCAAGCACA ACCTCGAACC CACAGAGAAC CTGGTCGGCC GGCGCAGAGG GAGTCGTAAG 
GACAAGGAGA CGGTTCGAGA CGACCTGGGC CAGAACCAGA ACATGGGTCT GGACGGCGAG 
GGGAAGGGCA AGAGCGTGGG CGACGGCAAA CAGAGGAAGC TCTCTCCTCT TCGCCTCTCT 
ATCTCAAACA ACCAGGATCC TGAGTTATAT GAGGATCTAG AAGACAAAAC CTCCGTTAGC 
AATGAAGTAG AGATGGAGTC AGAGGAGCAG ATTGCAGAGA GGAGGAGGAA GATGGCCAAC 
CCAGCGGAGC AGTCCCATCT CCCTGTCGGG GCGGCTTCCA GCTGGAAAGA ACTGAAACAC 
AAGGAGGTAA ACTTTACTCC ACCCATCTAC CAGCCTCCCC ACCACCACCT CTCCCCCAAA 
TCTCCCAGAG AGACACGAGA GGAGAGGAAG ATGGAGGCCA TCCTGCAAGC GTTTGCCCGA 
ATGGAGAAGA GAGAGAAGCG AAGGGAGCAG GCGCTGGAAA GAATCGGTAG CATCAAGTCG 
GAGGTCGGGG GACGCAGTGA CATCAAGGAG GAGCCTCCAG CCACGCCAGA GACGGCAGAT 
TCTCCAGCTG TTATGCAGCC GCTCCTGGAG GTGAAAGAGG AGCCAGGATT AAAGCCGGCC 
AAGGTCAAAG GCTCGAGGAA CAGGAAAAGT TTCTCAAGGA ACCGCACGCA CATTGGCCAA 
CAGCGCCGGA GAGCTCGCAC CATCAGCACC TGCTCTGACC TGCAGCCTGG CTCTCCAATT 
GAATCTGTGG AGCCTCTGAG CAACGAAGCC CCCGAAGGAG AGACACCCAC CGCACCAGAG 
CCGGAGGCAA TCTCTTCCCA AGCACCAGAC ACCAGCCCTC CGCACAGCAG CTCACCTGCA 
CCTGACAGAA ACCGCACCGG GAGCAAGAAC TTCAAAACTA AAAAGCACTT TGTGAGTGAG 
TGGGTGGGAG AGAAGCAGCA GGACCGTGGT GCAGTACGAA CCCCAGAGCC GGCCCCCGAG 
AGGCCGCTGA GAATAAGCAG TGACCCCGAA GTACTCGCCA CACAGCTGAA CGCCCTCCCA 
GGCATGGCCT GCTCTCCACA GGTCTACAGC ACGCCCAAAC ACTACGTCCG CTTCTCGTCC 
CCTTTCCTGG CGAACCGCAG TCCGACAACT CCCGGAGTCC CCACTGGGAG ACGGCGTTCC 
AGAGAACTGC CAGAAACGCC GCCAACCACC GGCTCCTGCA AGAAGCGATG GTTGAAGCAG 
GCCCTTGAGG AAGAGGGATC CACCAGCCCA GCCAGACGAC CAAGCCTCCT CATGCCCTGT 
GAGGGTCCTC TTAGCCCCTC AATCAACGGA GACTCTGACA GCCCTCTTCC CTACAATGGC 
ACCTGCTCGC TACCTGAGTT GCCCACGCCT TTGAAAAAGC GCCGGTTGAG TCCATTAGAT 
GCCTGTATGT CTGAGAGCTC CACACCCTAC GGCTCCCCTT GTGCCACGCC CACCAGGGCA 
GACCAATCGG AGACACCCGC AACCCCCATC TTACTGGCTA CCCCACCGCG TCCCAGAACG 
GAGGAGCCCA GTACAGAGCC CCCGCCCAAC ACCCCAACGC AGACACTTAA CGCCCCTCTG 
GAGAGTGAAT CCTCTGTGGA GAGCTCCCCA GAGCTCAGCC GGAAACCCAG TGTACAAGAG 
GCCGATCGTC CTCCTTCGTT GGTCTCCTCT CCCTGCATCA GGGCTCCCAG TTCAGACGGA 
CTCCCGGCTG AAGCCAAAGT GTCGGTTCCT GAGAGTCCAC AGCCTCCGGT CGCCGACTCT 
GTGGACTGTG GAGAGGACAG GACCGATGGT GGGGTTGTAG AAGGCAGTAG TGAGCCTTCC 
TCGTCCGCAG AAACATGTGC TTCCTCTTTT CCTGGCTGGA TAAAAAGCCC AGACAGAGGC 
CCGACTGGAC CAGCTGGCCT GAACTTCTCT CCAGTCAACT CAAACCTAAG GGACCTCACC 
CCCTCACACA CTCTGGAGCC CCTGGCGGCT CCCTTCAGGC CCGACGCTGC AGCTGGGGCT 
GCGGCAGGGT CCACAGCAGG GACCGGGTCA TTGGTTGGCC CTCAAGCCCC CTTCAGTGAA 
GGCCAGGGGC AGCTGTTTTA CCCCTGCTCT GAGGAGGGAG GTTCACTCGG CTTCTCGCGC 
TCACTGAATG GGGATGGCAG CGGTGAAGGA GGAGGATCAG CGCAGAACCC CCCACAGAAG 
AAAAAGGTTT CCCTGCTAGA ATACAGGAAA CGTCAGCGCG AAGCCCGTCG CAGCGGCTCC 
AAGACCGAGT GCAGCTCTCC TGTCTCCACA GTGCCACCTC TGACCGTGGA CGCCTTCCCC 
GTCGCGCTGG AGACGACAAG CGAACCTCCA CTACCCCCTG CTCCTACAGC TGTCTGCAAC 
GCCAACAACA CCATCGTTGT TAAAGAGCCC CAGACGAGCG AGGAGGCCGA GACGCCCGGA 
GAGAAAGGCG AGAAGGAAGG AGGAGAAGGA CAGTGGACGT CGTCCACTTC TGTAGAGCAG 
GCCCGAGAAC GCAGCTACCA CAGAGCTCTG CTGCTCAGCA AAGACAAGGA CACGGATGGT 
GAGACAGAAG GTGGAGATAC ACCTGCACTG AGAGATTGCC CGTCTCCAAG TCTTCAGAAG 
ACCCCGACTC ACACCCCCTG CTCTCCTGGT CCTCTCGCTC AGTCCACCAA TCGGCCACCG 
AAGGAGGAAG ACGGCGACGG GCAGCCGAGC AAGCCTGCGG GACCCAAGTC GGCTCCTCTG 
ACCCCCACAA AGCTGCATCC CACCCCGTTA CCCTCCTCGC CGGTCCACTA TCCGGGACCC 
TCCCTCCTGC ACTCGCCCAA GCCTCAGCCT CAGGGCTCGC CGTACCGCAG CCAGAGGGCG 
CTGTTCTCCG CTCAGCCTCA AAACCAACCG CAGCCCCAGG CTCAGACTGG CCCAGCTCCT 
TTCCCCCAGT ATAACACACA GAGTGCTCCA CCGCCACCTC CCCCTCCTCC ACCAGCACCA 
CCGGCCTCCA CGGCGTATTT TCCCAGCCAG AGCCCCTCGC CTGCTGGACC TTTCCCCGGG 
TTCAAACCCG CTGTCACACC CCCTTACCCT CCGTGTTCTC AGCCCCTGAT GCAGACTCTT 
CCCCACAGCG TGCACTATCA GAGCTCAGCC GCTCCACCGC CGCCGCCTCC TCCGCCCCCG 
CACCCGATGC CCGGCCCCAC CCTGCTGCAC GTCAACCTGC AGCCCCCTCC CATCCAGCAG 
CACCAGCTCA TGCTGACCAC CGCCCCTCCG CCGCCTCCTC CCCCACAGGG CCAGACCTCA 
CAGCAGCAGC AGCAGCAGCC TCCCGCTGGC AGCTCCCTGC TGTCGCTCAC CCCTCCTCCG 
CCGCCTCCTC CACCTCCTCC AGCCCCCTCG ACCAACGCCC CGATGCAGGC CCACCACTTT 
CAGAACTTAG GGGGTTTTCA GCCGGCGTTG CTACACCCGG GCGGCGCCGC CACAAACCCT 
TCAGTGCCCC AATCCACGTA CCCCCCACCC CTTCAGCAGA CCGGACTGCC TCCACCTCCC 
CCTCCTCCTC CCCAACAGAC TCAACAAGGC CAGGCTCAGG CCGCCGCCTC CCAGATGCCT 
TCTGGGACTC GTGGAGCTCC TGCGTCCTCG ACCCCCTTCC ACAGCTCGGG GTACCTGAGC 
ACAGGGTGGC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kmt2e ( XM_010738910.3 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010737212.3 Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OLa211865
Clone ID Related Accession (Same CDS sequence)	XM_010738910.3
Accession Version	XM_010738910.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	inactive histone-lysine N-methyltransferase 2E isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040030.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_010738910.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACAGCAGACA CCTCATACCT GGACATGGCT 
GCGGGCTCAG ACAGACCAGA ATCGGTGGAG GCCAGCCCTG TGGTGGTGGA AAAGTCCAGC 
TACCCACACC AGATCTACAG CAGCAGTTCT CACCATTCCC ACAGTTACAT TGGCCTGCCT 
TACGCTGACC ATAACTATGG GGCGCGGCCC CCACCTACAC CACCAGCCTC CCCTCCCCCC 
TCCATGTTGA TCCGACAAGG AGAGGGGGGG TTGTTTGTTC CAGGGGGCCA GGACGAAGCA 
TCAAGGGGCA CGACGCTCAG CACCTCAGAG GATGGCAGCT ACGGGGCTGA CATCACCCGC 
TGCATCTGTG GCTTCACCCA CGATGACGGC TACATGATCT GCTGTGATAA GTGCAGTGCC 
TGGCAGCATA TAGACTGCAT GGGCATCGAC AGGCAGAACA TTCCTGAGAC CTACCTGTGT 
GAACGCTGCC AACCACGACA CCTGGATCGA GAGAGGGCCA TCCTGCTGCA GACCAGGAAA 
CGGGAATGTT TGTCTGATGG GGACACGAGT GCTACAGAGA GTGGAGATGA GGTGCCGCTA 
GAGCTCTACT CCACCTTCCA ACACACGCCT ACCACCATCA CCCTGACTAC CGCGCGCCTT 
GGCAATAAGC AGGCTGATAA GAAACGCAAA AAGAGCGGTG ATAAAGAGCC TCCAGCGTCT 
TCTGCCCGAG CAAAGAAGGC CTTCCGAGAA GGGTCCAGAA AGTCCTCCAG AGTAAAGGGT 
GCAGCGCCAG AGCAGGAGCC AACAGAGTCC CTGTGGGAGA ACAAGATCAA GACGTGGATG 
GAGCGTTATG AGGAGGCCAG CAGCAATCAG TACAGCGAGG ATGTCCAGGT CCTGTTGCGT 
GTCAAAGAAC AAGGCGATGG CAAGAGCCTG GCGTACAACA CGCACCCTGC CTCTTTCAAA 
CCCCCTGTGG AGAGTCAAGT TCAGAAGAAC AAGAAGATCC TCAAAGCGGT GCGAGACTTG 
GCCCCAGACT CCCTCATCAT TGAGTACAGG GGCAAGTTCA TGCTCAGACA GCAGTTTGAG 
GCCAACGGAT ACTTCTTTAA GAGGCCATAC CCATTTGTGT TATTTTATTC TAAATTTGAC 
GGACTGGAGA TGTGCGTGGA CGCTCGTAGC TTCGGCAACG AAGCGCGCTT CATCCGTCGC 
TCCTGCACCC CTAATTCTGA AGTGCGGCAC GTCATTGAGG ACGGCATGCT GCATTTATAC 
ATCTACTCAT TGAGGCCGAT CACCAAGGGC ACAGAGATCA CCATCGGTTT TGATTTTGAC 
TATGGCAGCT GTAAATACAA GGTGGACTGT GCCTGCGTGA AGGGGAATCA GGAGTGCCCG 
GTGCTCAAGC ACAACCTCGA ACCCACAGAG AACCTGGTCG GCCGGCGCAG AGGGAGTCGT 
AAGGACAAGG AGACGGTTCG AGACGACCTG GGCCAGAACC AGAACATGGG TCTGGACGGC 
GAGGGGAAGG GCAAGAGCGT GGGCGACGGC AAACAGAGGA AGCTCTCTCC TCTTCGCCTC 
TCTATCTCAA ACAACCAGGA TCCTGAGTTA TATGAGGATC TAGAAGACAA AACCTCCGTT 
AGCAATGAAG TAGAGATGGA GTCAGAGGAG CAGATTGCAG AGAGGAGGAG GAAGATGGCC 
AACCCAGCGG AGCAGTCCCA TCTCCCTGTC GGGGCGGCTT CCAGCTGGAA AGAACTGAAA 
CACAAGGAGA CACGAGAGGA GAGGAAGATG GAGGCCATCC TGCAAGCGTT TGCCCGAATG 
GAGAAGAGAG AGAAGCGAAG GGAGCAGGCG CTGGAAAGAA TCGGTAGCAT CAAGTCGGAG 
GTCGGGGGAC GCAGTGACAT CAAGGAGGAG CCTCCAGCCA CGCCAGAGAC GGCAGATTCT 
CCAGCTGTTA TGCAGCCGCT CCTGGAGGTG AAAGAGGAGC CAGGATTAAA GCCGGCCAAG 
GTCAAAGGCT CGAGGAACAG GAAAAGTTTC TCAAGGAACC GCACGCACAT TGGCCAACAG 
CGCCGGAGAG CTCGCACCAT CAGCACCTGC TCTGACCTGC AGCCTGGCTC TCCAATTGAA 
TCTGTGGAGC CTCTGAGCAA CGAAGCCCCC GAAGGAGAGA CACCCACCGC ACCAGAGCCG 
GAGGCAATCT CTTCCCAAGC ACCAGACACC AGCCCTCCGC ACAGCAGCTC ACCTGCACCT 
GACAGAAACC GCACCGGGAG CAAGAACTTC AAAACTAAAA AGCACTTTGT GAGTGAGTGG 
GTGGGAGAGA AGCAGCAGGA CCGTGGTGCA GTACGAACCC CAGAGCCGGC CCCCGAGAGG 
CCGCTGAGAA TAAGCAGTGA CCCCGAAGTA CTCGCCACAC AGCTGAACGC CCTCCCAGGC 
ATGGCCTGCT CTCCACAGGT CTACAGCACG CCCAAACACT ACGTCCGCTT CTCGTCCCCT 
TTCCTGGCGA ACCGCAGTCC GACAACTCCC GGAGTCCCCA CTGGGAGACG GCGTTCCAGA 
GAACTGCCAG AAACGCCGCC AACCACCGGC TCCTGCAAGA AGCGATGGTT GAAGCAGGCC 
CTTGAGGAAG AGGGATCCAC CAGCCCAGCC AGACGACCAA GCCTCCTCAT GCCCTGTGAG 
GGTCCTCTTA GCCCCTCAAT CAACGGAGAC TCTGACAGCC CTCTTCCCTA CAATGGCACC 
TGCTCGCTAC CTGAGTTGCC CACGCCTTTG AAAAAGCGCC GGTTGAGTCC ATTAGATGCC 
TGTATGTCTG AGAGCTCCAC ACCCTACGGC TCCCCTTGTG CCACGCCCAC CAGGGCAGAC 
CAATCGGAGA CACCCGCAAC CCCCATCTTA CTGGCTACCC CACCGCGTCC CAGAACGGAG 
GAGCCCAGTA CAGAGCCCCC GCCCAACACC CCAACGCAGA CACTTAACGC CCCTCTGGAG 
AGTGAATCCT CTGTGGAGAG CTCCCCAGAG CTCAGCCGGA AACCCAGTGT ACAAGAGGCC 
GATCGTCCTC CTTCGTTGGT CTCCTCTCCC TGCATCAGGG CTCCCAGTTC AGACGGACTC 
CCGGCTGAAG CCAAAGTGTC GGTTCCTGAG AGTCCACAGC CTCCGGTCGC CGACTCTGTG 
GACTGTGGAG AGGACAGGAC CGATGGTGGG GTTGTAGAAG GCAGTAGTGA GCCTTCCTCG 
TCCGCAGAAA CATGTGCTTC CTCTTTTCCT GGCTGGATAA AAAGCCCAGA CAGAGGCCCG 
ACTGGACCAG CTGGCCTGAA CTTCTCTCCA GTCAACTCAA ACCTAAGGGA CCTCACCCCC 
TCACACACTC TGGAGCCCCT GGCGGCTCCC TTCAGGCCCG ACGCTGCAGC TGGGGCTGCG 
GCAGGGTCCA CAGCAGGGAC CGGGTCATTG GTTGGCCCTC AAGCCCCCTT CAGTGAAGGC 
CAGGGGCAGC TGTTTTACCC CTGCTCTGAG GAGGGAGGTT CACTCGGCTT CTCGCGCTCA 
CTGAATGGGG ATGGCAGCGG TGAAGGAGGA GGATCAGCGC AGAACCCCCC ACAGAAGAAA 
AAGGTTTCCC TGCTAGAATA CAGGAAACGT CAGCGCGAAG CCCGTCGCAG CGGCTCCAAG 
ACCGAGTGCA GCTCTCCTGT CTCCACAGTG CCACCTCTGA CCGTGGACGC CTTCCCCGTC 
GCGCTGGAGA CGACAAGCGA ACCTCCACTA CCCCCTGCTC CTACAGCTGT CTGCAACGCC 
AACAACACCA TCGTTGTTAA AGAGCCCCAG ACGAGCGAGG AGGCCGAGAC GCCCGGAGAG 
AAAGGCGAGA AGGAAGGAGG AGAAGGACAG TGGACGTCGT CCACTTCTGT AGAGCAGGCC 
CGAGAACGCA GCTACCACAG AGCTCTGCTG CTCAGCAAAG ACAAGGACAC GGATGGTGAG 
ACAGAAGGTG GAGATACACC TGCACTGAGA GATTGCCCGT CTCCAAGTCT TCAGAAGACC 
CCGACTCACA CCCCCTGCTC TCCTGGTCCT CTCGCTCAGT CCACCAATCG GCCACCGAAG 
GAGGAAGACG GCGACGGGCA GCCGAGCAAG CCTGCGGGAC CCAAGTCGGC TCCTCTGACC 
CCCACAAAGC TGCATCCCAC CCCGTTACCC TCCTCGCCGG TCCACTATCC GGGACCCTCC 
CTCCTGCACT CGCCCAAGCC TCAGCCTCAG GGCTCGCCGT ACCGCAGCCA GAGGGCGCTG 
TTCTCCGCTC AGCCTCAAAA CCAACCGCAG CCCCAGGCTC AGACTGGCCC AGCTCCTTTC 
CCCCAGTATA ACACACAGAG TGCTCCACCG CCACCTCCCC CTCCTCCACC AGCACCACCG 
GCCTCCACGG CGTATTTTCC CAGCCAGAGC CCCTCGCCTG CTGGACCTTT CCCCGGGTTC 
AAACCCGCTG TCACACCCCC TTACCCTCCG TGTTCTCAGC CCCTGATGCA GACTCTTCCC 
CACAGCGTGC ACTATCAGAG CTCAGCCGCT CCACCGCCGC CGCCTCCTCC GCCCCCGCAC 
CCGATGCCCG GCCCCACCCT GCTGCACGTC AACCTGCAGC CCCCTCCCAT CCAGCAGCAC 
CAGCTCATGC TGACCACCGC CCCTCCGCCG CCTCCTCCCC CACAGGGCCA GACCTCACAG 
CAGCAGCAGC AGCAGCCTCC CGCTGGCAGC TCCCTGCTGT CGCTCACCCC TCCTCCGCCG 
CCTCCTCCAC CTCCTCCAGC CCCCTCGACC AACGCCCCGA TGCAGGCCCA CCACTTTCAG 
AACTTAGGGG GTTTTCAGCC GGCGTTGCTA CACCCGGGCG GCGCCGCCAC AAACCCTTCA 
GTGCCCCAAT CCACGTACCC CCCACCCCTT CAGCAGACCG GACTGCCTCC ACCTCCCCCT 
CCTCCTCCCC AACAGACTCA ACAAGGCCAG GCTCAGGCCG CCGCCTCCCA GATGCCTTCT 
GGGACTCGTG GAGCTCCTGC GTCCTCGACC CCCTTCCACA GCTCGGGGTA CCTGAGCACA 
GGGTGGCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010737212.3
CDS	483..5474
Translation	MSIVIPVGVDTADTSYLDMAAGSDRPESVEASPVVVEKSSYPHQIYSSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPSMLIRQGEGGLFVPGGQDEASRGTTLSTSEDGSYGADITRCICGFTHDDGYMICCDKCSAWQHIDCMGIDRQNIPETYLCERCQPRHLDRERAILLQTRKRECLSDGDTSATESGDEVPLELYSTFQHTPTTITLTTARLGNKQADKKRKKSGDKEPPASSARAKKAFREGSRKSSRVKGAAPEQEPTESLWENKIKTWMERYEEASSNQYSEDVQVLLRVKEQGDGKSLAYNTHPASFKPPVESQVQKNKKILKAVRDLAPDSLIIEYRGKFMLRQQFEANGYFFKRPYPFVLFYSKFDGLEMCVDARSFGNEARFIRRSCTPNSEVRHVIEDGMLHLYIYSLRPITKGTEITIGFDFDYGSCKYKVDCACVKGNQECPVLKHNLEPTENLVGRRRGSRKDKETVRDDLGQNQNMGLDGEGKGKSVGDGKQRKLSPLRLSISNNQDPELYEDLEDKTSVSNEVEMESEEQIAERRRKMANPAEQSHLPVGAASSWKELKHKETREERKMEAILQAFARMEKREKRREQALERIGSIKSEVGGRSDIKEEPPATPETADSPAVMQPLLEVKEEPGLKPAKVKGSRNRKSFSRNRTHIGQQRRRARTISTCSDLQPGSPIESVEPLSNEAPEGETPTAPEPEAISSQAPDTSPPHSSSPAPDRNRTGSKNFKTKKHFVSEWVGEKQQDRGAVRTPEPAPERPLRISSDPEVLATQLNALPGMACSPQVYSTPKHYVRFSSPFLANRSPTTPGVPTGRRRSRELPETPPTTGSCKKRWLKQALEEEGSTSPARRPSLLMPCEGPLSPSINGDSDSPLPYNGTCSLPELPTPLKKRRLSPLDACMSESSTPYGSPCATPTRADQSETPATPILLATPPRPRTEEPSTEPPPNTPTQTLNAPLESESSVESSPELSRKPSVQEADRPPSLVSSPCIRAPSSDGLPAEAKVSVPESPQPPVADSVDCGEDRTDGGVVEGSSEPSSSAETCASSFPGWIKSPDRGPTGPAGLNFSPVNSNLRDLTPSHTLEPLAAPFRPDAAAGAAAGSTAGTGSLVGPQAPFSEGQGQLFYPCSEEGGSLGFSRSLNGDGSGEGGGSAQNPPQKKKVSLLEYRKRQREARRSGSKTECSSPVSTVPPLTVDAFPVALETTSEPPLPPAPTAVCNANNTIVVKEPQTSEEAETPGEKGEKEGGEGQWTSSTSVEQARERSYHRALLLSKDKDTDGETEGGDTPALRDCPSPSLQKTPTHTPCSPGPLAQSTNRPPKEEDGDGQPSKPAGPKSAPLTPTKLHPTPLPSSPVHYPGPSLLHSPKPQPQGSPYRSQRALFSAQPQNQPQPQAQTGPAPFPQYNTQSAPPPPPPPPPAPPASTAYFPSQSPSPAGPFPGFKPAVTPPYPPCSQPLMQTLPHSVHYQSSAAPPPPPPPPPHPMPGPTLLHVNLQPPPIQQHQLMLTTAPPPPPPPQGQTSQQQQQQPPAGSSLLSLTPPPPPPPPPPAPSTNAPMQAHHFQNLGGFQPALLHPGGAATNPSVPQSTYPPPLQQTGLPPPPPPPPQQTQQGQAQAAASQMPSGTRGAPASSTPFHSSGYLSTGWH

Target ORF information:

RefSeq Version	XM_010738910.3
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010738910.3

ORF Insert Sequence:

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACAGCAGACA CCTCATACCT GGACATGGCT 
GCGGGCTCAG ACAGACCAGA ATCGGTGGAG GCCAGCCCTG TGGTGGTGGA AAAGTCCAGC 
TACCCACACC AGATCTACAG CAGCAGTTCT CACCATTCCC ACAGTTACAT TGGCCTGCCT 
TACGCTGACC ATAACTATGG GGCGCGGCCC CCACCTACAC CACCAGCCTC CCCTCCCCCC 
TCCATGTTGA TCCGACAAGG AGAGGGGGGG TTGTTTGTTC CAGGGGGCCA GGACGAAGCA 
TCAAGGGGCA CGACGCTCAG CACCTCAGAG GATGGCAGCT ACGGGGCTGA CATCACCCGC 
TGCATCTGTG GCTTCACCCA CGATGACGGC TACATGATCT GCTGTGATAA GTGCAGTGCC 
TGGCAGCATA TAGACTGCAT GGGCATCGAC AGGCAGAACA TTCCTGAGAC CTACCTGTGT 
GAACGCTGCC AACCACGACA CCTGGATCGA GAGAGGGCCA TCCTGCTGCA GACCAGGAAA 
CGGGAATGTT TGTCTGATGG GGACACGAGT GCTACAGAGA GTGGAGATGA GGTGCCGCTA 
GAGCTCTACT CCACCTTCCA ACACACGCCT ACCACCATCA CCCTGACTAC CGCGCGCCTT 
GGCAATAAGC AGGCTGATAA GAAACGCAAA AAGAGCGGTG ATAAAGAGCC TCCAGCGTCT 
TCTGCCCGAG CAAAGAAGGC CTTCCGAGAA GGGTCCAGAA AGTCCTCCAG AGTAAAGGGT 
GCAGCGCCAG AGCAGGAGCC AACAGAGTCC CTGTGGGAGA ACAAGATCAA GACGTGGATG 
GAGCGTTATG AGGAGGCCAG CAGCAATCAG TACAGCGAGG ATGTCCAGGT CCTGTTGCGT 
GTCAAAGAAC AAGGCGATGG CAAGAGCCTG GCGTACAACA CGCACCCTGC CTCTTTCAAA 
CCCCCTGTGG AGAGTCAAGT TCAGAAGAAC AAGAAGATCC TCAAAGCGGT GCGAGACTTG 
GCCCCAGACT CCCTCATCAT TGAGTACAGG GGCAAGTTCA TGCTCAGACA GCAGTTTGAG 
GCCAACGGAT ACTTCTTTAA GAGGCCATAC CCATTTGTGT TATTTTATTC TAAATTTGAC 
GGACTGGAGA TGTGCGTGGA CGCTCGTAGC TTCGGCAACG AAGCGCGCTT CATCCGTCGC 
TCCTGCACCC CTAATTCTGA AGTGCGGCAC GTCATTGAGG ACGGCATGCT GCATTTATAC 
ATCTACTCAT TGAGGCCGAT CACCAAGGGC ACAGAGATCA CCATCGGTTT TGATTTTGAC 
TATGGCAGCT GTAAATACAA GGTGGACTGT GCCTGCGTGA AGGGGAATCA GGAGTGCCCG 
GTGCTCAAGC ACAACCTCGA ACCCACAGAG AACCTGGTCG GCCGGCGCAG AGGGAGTCGT 
AAGGACAAGG AGACGGTTCG AGACGACCTG GGCCAGAACC AGAACATGGG TCTGGACGGC 
GAGGGGAAGG GCAAGAGCGT GGGCGACGGC AAACAGAGGA AGCTCTCTCC TCTTCGCCTC 
TCTATCTCAA ACAACCAGGA TCCTGAGTTA TATGAGGATC TAGAAGACAA AACCTCCGTT 
AGCAATGAAG TAGAGATGGA GTCAGAGGAG CAGATTGCAG AGAGGAGGAG GAAGATGGCC 
AACCCAGCGG AGCAGTCCCA TCTCCCTGTC GGGGCGGCTT CCAGCTGGAA AGAACTGAAA 
CACAAGGAGA CACGAGAGGA GAGGAAGATG GAGGCCATCC TGCAAGCGTT TGCCCGAATG 
GAGAAGAGAG AGAAGCGAAG GGAGCAGGCG CTGGAAAGAA TCGGTAGCAT CAAGTCGGAG 
GTCGGGGGAC GCAGTGACAT CAAGGAGGAG CCTCCAGCCA CGCCAGAGAC GGCAGATTCT 
CCAGCTGTTA TGCAGCCGCT CCTGGAGGTG AAAGAGGAGC CAGGATTAAA GCCGGCCAAG 
GTCAAAGGCT CGAGGAACAG GAAAAGTTTC TCAAGGAACC GCACGCACAT TGGCCAACAG 
CGCCGGAGAG CTCGCACCAT CAGCACCTGC TCTGACCTGC AGCCTGGCTC TCCAATTGAA 
TCTGTGGAGC CTCTGAGCAA CGAAGCCCCC GAAGGAGAGA CACCCACCGC ACCAGAGCCG 
GAGGCAATCT CTTCCCAAGC ACCAGACACC AGCCCTCCGC ACAGCAGCTC ACCTGCACCT 
GACAGAAACC GCACCGGGAG CAAGAACTTC AAAACTAAAA AGCACTTTGT GAGTGAGTGG 
GTGGGAGAGA AGCAGCAGGA CCGTGGTGCA GTACGAACCC CAGAGCCGGC CCCCGAGAGG 
CCGCTGAGAA TAAGCAGTGA CCCCGAAGTA CTCGCCACAC AGCTGAACGC CCTCCCAGGC 
ATGGCCTGCT CTCCACAGGT CTACAGCACG CCCAAACACT ACGTCCGCTT CTCGTCCCCT 
TTCCTGGCGA ACCGCAGTCC GACAACTCCC GGAGTCCCCA CTGGGAGACG GCGTTCCAGA 
GAACTGCCAG AAACGCCGCC AACCACCGGC TCCTGCAAGA AGCGATGGTT GAAGCAGGCC 
CTTGAGGAAG AGGGATCCAC CAGCCCAGCC AGACGACCAA GCCTCCTCAT GCCCTGTGAG 
GGTCCTCTTA GCCCCTCAAT CAACGGAGAC TCTGACAGCC CTCTTCCCTA CAATGGCACC 
TGCTCGCTAC CTGAGTTGCC CACGCCTTTG AAAAAGCGCC GGTTGAGTCC ATTAGATGCC 
TGTATGTCTG AGAGCTCCAC ACCCTACGGC TCCCCTTGTG CCACGCCCAC CAGGGCAGAC 
CAATCGGAGA CACCCGCAAC CCCCATCTTA CTGGCTACCC CACCGCGTCC CAGAACGGAG 
GAGCCCAGTA CAGAGCCCCC GCCCAACACC CCAACGCAGA CACTTAACGC CCCTCTGGAG 
AGTGAATCCT CTGTGGAGAG CTCCCCAGAG CTCAGCCGGA AACCCAGTGT ACAAGAGGCC 
GATCGTCCTC CTTCGTTGGT CTCCTCTCCC TGCATCAGGG CTCCCAGTTC AGACGGACTC 
CCGGCTGAAG CCAAAGTGTC GGTTCCTGAG AGTCCACAGC CTCCGGTCGC CGACTCTGTG 
GACTGTGGAG AGGACAGGAC CGATGGTGGG GTTGTAGAAG GCAGTAGTGA GCCTTCCTCG 
TCCGCAGAAA CATGTGCTTC CTCTTTTCCT GGCTGGATAA AAAGCCCAGA CAGAGGCCCG 
ACTGGACCAG CTGGCCTGAA CTTCTCTCCA GTCAACTCAA ACCTAAGGGA CCTCACCCCC 
TCACACACTC TGGAGCCCCT GGCGGCTCCC TTCAGGCCCG ACGCTGCAGC TGGGGCTGCG 
GCAGGGTCCA CAGCAGGGAC CGGGTCATTG GTTGGCCCTC AAGCCCCCTT CAGTGAAGGC 
CAGGGGCAGC TGTTTTACCC CTGCTCTGAG GAGGGAGGTT CACTCGGCTT CTCGCGCTCA 
CTGAATGGGG ATGGCAGCGG TGAAGGAGGA GGATCAGCGC AGAACCCCCC ACAGAAGAAA 
AAGGTTTCCC TGCTAGAATA CAGGAAACGT CAGCGCGAAG CCCGTCGCAG CGGCTCCAAG 
ACCGAGTGCA GCTCTCCTGT CTCCACAGTG CCACCTCTGA CCGTGGACGC CTTCCCCGTC 
GCGCTGGAGA CGACAAGCGA ACCTCCACTA CCCCCTGCTC CTACAGCTGT CTGCAACGCC 
AACAACACCA TCGTTGTTAA AGAGCCCCAG ACGAGCGAGG AGGCCGAGAC GCCCGGAGAG 
AAAGGCGAGA AGGAAGGAGG AGAAGGACAG TGGACGTCGT CCACTTCTGT AGAGCAGGCC 
CGAGAACGCA GCTACCACAG AGCTCTGCTG CTCAGCAAAG ACAAGGACAC GGATGGTGAG 
ACAGAAGGTG GAGATACACC TGCACTGAGA GATTGCCCGT CTCCAAGTCT TCAGAAGACC 
CCGACTCACA CCCCCTGCTC TCCTGGTCCT CTCGCTCAGT CCACCAATCG GCCACCGAAG 
GAGGAAGACG GCGACGGGCA GCCGAGCAAG CCTGCGGGAC CCAAGTCGGC TCCTCTGACC 
CCCACAAAGC TGCATCCCAC CCCGTTACCC TCCTCGCCGG TCCACTATCC GGGACCCTCC 
CTCCTGCACT CGCCCAAGCC TCAGCCTCAG GGCTCGCCGT ACCGCAGCCA GAGGGCGCTG 
TTCTCCGCTC AGCCTCAAAA CCAACCGCAG CCCCAGGCTC AGACTGGCCC AGCTCCTTTC 
CCCCAGTATA ACACACAGAG TGCTCCACCG CCACCTCCCC CTCCTCCACC AGCACCACCG 
GCCTCCACGG CGTATTTTCC CAGCCAGAGC CCCTCGCCTG CTGGACCTTT CCCCGGGTTC 
AAACCCGCTG TCACACCCCC TTACCCTCCG TGTTCTCAGC CCCTGATGCA GACTCTTCCC 
CACAGCGTGC ACTATCAGAG CTCAGCCGCT CCACCGCCGC CGCCTCCTCC GCCCCCGCAC 
CCGATGCCCG GCCCCACCCT GCTGCACGTC AACCTGCAGC CCCCTCCCAT CCAGCAGCAC 
CAGCTCATGC TGACCACCGC CCCTCCGCCG CCTCCTCCCC CACAGGGCCA GACCTCACAG 
CAGCAGCAGC AGCAGCCTCC CGCTGGCAGC TCCCTGCTGT CGCTCACCCC TCCTCCGCCG 
CCTCCTCCAC CTCCTCCAGC CCCCTCGACC AACGCCCCGA TGCAGGCCCA CCACTTTCAG 
AACTTAGGGG GTTTTCAGCC GGCGTTGCTA CACCCGGGCG GCGCCGCCAC AAACCCTTCA 
GTGCCCCAAT CCACGTACCC CCCACCCCTT CAGCAGACCG GACTGCCTCC ACCTCCCCCT 
CCTCCTCCCC AACAGACTCA ACAAGGCCAG GCTCAGGCCG CCGCCTCCCA GATGCCTTCT 
GGGACTCGTG GAGCTCCTGC GTCCTCGACC CCCTTCCACA GCTCGGGGTA CCTGAGCACA 
GGGTGGCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kmt2e ( XM_019278449.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019133994.2 Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X4, mRNA.

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CloneID	OLa211866
Clone ID Related Accession (Same CDS sequence)	XM_019278449.2
Accession Version	XM_019278449.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	inactive histone-lysine N-methyltransferase 2E isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040030.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_019278449.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACAGCAGACA CCTCATACCT GGACATGGCT 
GCGGGCTCAG ACAGACCAGA ATCGGTGGAG GCCAGCCCTG TGGTGGTGGA AAAGTCCAGC 
TACCCACACC AGATCTACAG CAGCAGTTCT CACCATTCCC ACAGTTACAT TGGCCTGCCT 
TACGCTGACC ATAACTATGG GGCGCGGCCC CCACCTACAC CACCAGCCTC CCCTCCCCCC 
TCCATGTTGA TCCGACAAGG AGAGGGGGGG TTGTTTGTTC CAGGGGGCCA GGACGAAGCA 
TCAAGGGGCA CGACGCTCAG CACCTCAGAG GATGGCAGCT ACGGGGCTGA CATCACCCGC 
TGCATCTGTG GCTTCACCCA CGATGACGGC TACATGATCT GCTGTGATAA GTGCAGTGCC 
TGGCAGCATA TAGACTGCAT GGGCATCGAC AGGCAGAACA TTCCTGAGAC CTACCTGTGT 
GAACGCTGCC AACCACGACA CCTGGATCGA GAGAGGGCCA TCCTGCTGCA GACCAGGAAA 
CGGGAATGTT TGTCTGATGG GGACACGAGT GCTACAGAGA GTGGAGATGA GGTGCCGCTA 
GAGCTCTACT CCACCTTCCA ACACACGCCT ACCACCATCA CCCTGACTAC CGCGCGCCTT 
GGCAATAAGC AGGCTGATAA GAAACGCAAA AAGAGCGGTG ATAAAGAGCC TCCAGCGTCT 
TCTGCCCGAG CAAAGAAGGC CTTCCGAGAA GGGTCCAGAA AGTCCTCCAG AGTAAAGGGT 
GCAGCGCCAG AGCAGGAGCC AACAGAGTCC CTGTGGGAGA ACAAGATCAA GACGTGGATG 
GAGCGTTATG AGGAGGCCAG CAGCAATCAG TACAGCGAGG ATGTCCAGGT CCTGTTGCGT 
GTCAAAGAAC AAGGCGATGG CAAGAGCCTG GCGTACAACA CGCACCCTGC CTCTTTCAAA 
CCCCCTGTGG AGAGTCAAGT TCAGAAGAAC AAGAAGATCC TCAAAGCGGT GCGAGACTTG 
GCCCCAGACT CCCTCATCAT TGAGTACAGG GGCAAGTTCA TGCTCAGACA GCAGTTTGAG 
GCCAACGGAT ACTTCTTTAA GAGGCCATAC CCATTTGTGT TATTTTATTC TAAATTTGAC 
GGACTGGAGA TGTGCGTGGA CGCTCGTAGC TTCGGCAACG AAGCGCGCTT CATCCGTCGC 
TCCTGCACCC CTAATTCTGA AGTGCGGCAC GTCATTGAGG ACGGCATGCT GCATTTATAC 
ATCTACTCAT TGAGGCCGAT CACCAAGGGC ACAGAGATCA CCATCGGTTT TGATTTTGAC 
TATGGCAGCT GTAAATACAA GGTGGACTGT GCCTGCGTGA AGGGGAATCA GGAGTGCCCG 
GTGCTCAAGC ACAACCTCGA ACCCACAGAG AACCTGGTCG GCCGGCGCAG AGGGAGTCGT 
AAGGACAAGG AGACGGTTCG AGACGACCTG GGCCAGAACC AGAACATGGG TCTGGACGGC 
GAGGGGAAGG GCAAGAGCGT GGGCGACGGC AAACAGAGGA AGCTCTCTCC TCTTCGCCTC 
TCTATCTCAA ACAACCAGGC CAACCCAGCG GAGCAGTCCC ATCTCCCTGT CGGGGCGGCT 
TCCAGCTGGA AAGAACTGAA ACACAAGGAG GTAAACTTTA CTCCACCCAT CTACCAGCCT 
CCCCACCACC ACCTCTCCCC CAAATCTCCC AGAGAGACAC GAGAGGAGAG GAAGATGGAG 
GCCATCCTGC AAGCGTTTGC CCGAATGGAG AAGAGAGAGA AGCGAAGGGA GCAGGCGCTG 
GAAAGAATCG GTAGCATCAA GTCGGAGGTC GGGGGACGCA GTGACATCAA GGAGGAGCCT 
CCAGCCACGC CAGAGACGGC AGATTCTCCA GCTGTTATGC AGCCGCTCCT GGAGGTGAAA 
GAGGAGCCAG GATTAAAGCC GGCCAAGGTC AAAGGCTCGA GGAACAGGAA AAGTTTCTCA 
AGGAACCGCA CGCACATTGG CCAACAGCGC CGGAGAGCTC GCACCATCAG CACCTGCTCT 
GACCTGCAGC CTGGCTCTCC AATTGAATCT GTGGAGCCTC TGAGCAACGA AGCCCCCGAA 
GGAGAGACAC CCACCGCACC AGAGCCGGAG GCAATCTCTT CCCAAGCACC AGACACCAGC 
CCTCCGCACA GCAGCTCACC TGCACCTGAC AGAAACCGCA CCGGGAGCAA GAACTTCAAA 
ACTAAAAAGC ACTTTGTGAG TGAGTGGGTG GGAGAGAAGC AGCAGGACCG TGGTGCAGTA 
CGAACCCCAG AGCCGGCCCC CGAGAGGCCG CTGAGAATAA GCAGTGACCC CGAAGTACTC 
GCCACACAGC TGAACGCCCT CCCAGGCATG GCCTGCTCTC CACAGGTCTA CAGCACGCCC 
AAACACTACG TCCGCTTCTC GTCCCCTTTC CTGGCGAACC GCAGTCCGAC AACTCCCGGA 
GTCCCCACTG GGAGACGGCG TTCCAGAGAA CTGCCAGAAA CGCCGCCAAC CACCGGCTCC 
TGCAAGAAGC GATGGTTGAA GCAGGCCCTT GAGGAAGAGG GATCCACCAG CCCAGCCAGA 
CGACCAAGCC TCCTCATGCC CTGTGAGGGT CCTCTTAGCC CCTCAATCAA CGGAGACTCT 
GACAGCCCTC TTCCCTACAA TGGCACCTGC TCGCTACCTG AGTTGCCCAC GCCTTTGAAA 
AAGCGCCGGT TGAGTCCATT AGATGCCTGT ATGTCTGAGA GCTCCACACC CTACGGCTCC 
CCTTGTGCCA CGCCCACCAG GGCAGACCAA TCGGAGACAC CCGCAACCCC CATCTTACTG 
GCTACCCCAC CGCGTCCCAG AACGGAGGAG CCCAGTACAG AGCCCCCGCC CAACACCCCA 
ACGCAGACAC TTAACGCCCC TCTGGAGAGT GAATCCTCTG TGGAGAGCTC CCCAGAGCTC 
AGCCGGAAAC CCAGTGTACA AGAGGCCGAT CGTCCTCCTT CGTTGGTCTC CTCTCCCTGC 
ATCAGGGCTC CCAGTTCAGA CGGACTCCCG GCTGAAGCCA AAGTGTCGGT TCCTGAGAGT 
CCACAGCCTC CGGTCGCCGA CTCTGTGGAC TGTGGAGAGG ACAGGACCGA TGGTGGGGTT 
GTAGAAGGCA GTAGTGAGCC TTCCTCGTCC GCAGAAACAT GTGCTTCCTC TTTTCCTGGC 
TGGATAAAAA GCCCAGACAG AGGCCCGACT GGACCAGCTG GCCTGAACTT CTCTCCAGTC 
AACTCAAACC TAAGGGACCT CACCCCCTCA CACACTCTGG AGCCCCTGGC GGCTCCCTTC 
AGGCCCGACG CTGCAGCTGG GGCTGCGGCA GGGTCCACAG CAGGGACCGG GTCATTGGTT 
GGCCCTCAAG CCCCCTTCAG TGAAGGCCAG GGGCAGCTGT TTTACCCCTG CTCTGAGGAG 
GGAGGTTCAC TCGGCTTCTC GCGCTCACTG AATGGGGATG GCAGCGGTGA AGGAGGAGGA 
TCAGCGCAGA ACCCCCCACA GAAGAAAAAG GTTTCCCTGC TAGAATACAG GAAACGTCAG 
CGCGAAGCCC GTCGCAGCGG CTCCAAGACC GAGTGCAGCT CTCCTGTCTC CACAGTGCCA 
CCTCTGACCG TGGACGCCTT CCCCGTCGCG CTGGAGACGA CAAGCGAACC TCCACTACCC 
CCTGCTCCTA CAGCTGTCTG CAACGCCAAC AACACCATCG TTGTTAAAGA GCCCCAGACG 
AGCGAGGAGG CCGAGACGCC CGGAGAGAAA GGCGAGAAGG AAGGAGGAGA AGGACAGTGG 
ACGTCGTCCA CTTCTGTAGA GCAGGCCCGA GAACGCAGCT ACCACAGAGC TCTGCTGCTC 
AGCAAAGACA AGGACACGGA TGGTGAGACA GAAGGTGGAG ATACACCTGC ACTGAGAGAT 
TGCCCGTCTC CAAGTCTTCA GAAGACCCCG ACTCACACCC CCTGCTCTCC TGGTCCTCTC 
GCTCAGTCCA CCAATCGGCC ACCGAAGGAG GAAGACGGCG ACGGGCAGCC GAGCAAGCCT 
GCGGGACCCA AGTCGGCTCC TCTGACCCCC ACAAAGCTGC ATCCCACCCC GTTACCCTCC 
TCGCCGGTCC ACTATCCGGG ACCCTCCCTC CTGCACTCGC CCAAGCCTCA GCCTCAGGGC 
TCGCCGTACC GCAGCCAGAG GGCGCTGTTC TCCGCTCAGC CTCAAAACCA ACCGCAGCCC 
CAGGCTCAGA CTGGCCCAGC TCCTTTCCCC CAGTATAACA CACAGAGTGC TCCACCGCCA 
CCTCCCCCTC CTCCACCAGC ACCACCGGCC TCCACGGCGT ATTTTCCCAG CCAGAGCCCC 
TCGCCTGCTG GACCTTTCCC CGGGTTCAAA CCCGCTGTCA CACCCCCTTA CCCTCCGTGT 
TCTCAGCCCC TGATGCAGAC TCTTCCCCAC AGCGTGCACT ATCAGAGCTC AGCCGCTCCA 
CCGCCGCCGC CTCCTCCGCC CCCGCACCCG ATGCCCGGCC CCACCCTGCT GCACGTCAAC 
CTGCAGCCCC CTCCCATCCA GCAGCACCAG CTCATGCTGA CCACCGCCCC TCCGCCGCCT 
CCTCCCCCAC AGGGCCAGAC CTCACAGCAG CAGCAGCAGC AGCCTCCCGC TGGCAGCTCC 
CTGCTGTCGC TCACCCCTCC TCCGCCGCCT CCTCCACCTC CTCCAGCCCC CTCGACCAAC 
GCCCCGATGC AGGCCCACCA CTTTCAGAAC TTAGGGGGTT TTCAGCCGGC GTTGCTACAC 
CCGGGCGGCG CCGCCACAAA CCCTTCAGTG CCCCAATCCA CGTACCCCCC ACCCCTTCAG 
CAGACCGGAC TGCCTCCACC TCCCCCTCCT CCTCCCCAAC AGACTCAACA AGGCCAGGCT 
CAGGCCGCCG CCTCCCAGAT GCCTTCTGGG ACTCGTGGAG CTCCTGCGTC CTCGACCCCC 
TTCCACAGCT CGGGGTACCT GAGCACAGGG TGGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019133994.2
CDS	484..5442
Translation	MSIVIPVGVDTADTSYLDMAAGSDRPESVEASPVVVEKSSYPHQIYSSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPSMLIRQGEGGLFVPGGQDEASRGTTLSTSEDGSYGADITRCICGFTHDDGYMICCDKCSAWQHIDCMGIDRQNIPETYLCERCQPRHLDRERAILLQTRKRECLSDGDTSATESGDEVPLELYSTFQHTPTTITLTTARLGNKQADKKRKKSGDKEPPASSARAKKAFREGSRKSSRVKGAAPEQEPTESLWENKIKTWMERYEEASSNQYSEDVQVLLRVKEQGDGKSLAYNTHPASFKPPVESQVQKNKKILKAVRDLAPDSLIIEYRGKFMLRQQFEANGYFFKRPYPFVLFYSKFDGLEMCVDARSFGNEARFIRRSCTPNSEVRHVIEDGMLHLYIYSLRPITKGTEITIGFDFDYGSCKYKVDCACVKGNQECPVLKHNLEPTENLVGRRRGSRKDKETVRDDLGQNQNMGLDGEGKGKSVGDGKQRKLSPLRLSISNNQANPAEQSHLPVGAASSWKELKHKEVNFTPPIYQPPHHHLSPKSPRETREERKMEAILQAFARMEKREKRREQALERIGSIKSEVGGRSDIKEEPPATPETADSPAVMQPLLEVKEEPGLKPAKVKGSRNRKSFSRNRTHIGQQRRRARTISTCSDLQPGSPIESVEPLSNEAPEGETPTAPEPEAISSQAPDTSPPHSSSPAPDRNRTGSKNFKTKKHFVSEWVGEKQQDRGAVRTPEPAPERPLRISSDPEVLATQLNALPGMACSPQVYSTPKHYVRFSSPFLANRSPTTPGVPTGRRRSRELPETPPTTGSCKKRWLKQALEEEGSTSPARRPSLLMPCEGPLSPSINGDSDSPLPYNGTCSLPELPTPLKKRRLSPLDACMSESSTPYGSPCATPTRADQSETPATPILLATPPRPRTEEPSTEPPPNTPTQTLNAPLESESSVESSPELSRKPSVQEADRPPSLVSSPCIRAPSSDGLPAEAKVSVPESPQPPVADSVDCGEDRTDGGVVEGSSEPSSSAETCASSFPGWIKSPDRGPTGPAGLNFSPVNSNLRDLTPSHTLEPLAAPFRPDAAAGAAAGSTAGTGSLVGPQAPFSEGQGQLFYPCSEEGGSLGFSRSLNGDGSGEGGGSAQNPPQKKKVSLLEYRKRQREARRSGSKTECSSPVSTVPPLTVDAFPVALETTSEPPLPPAPTAVCNANNTIVVKEPQTSEEAETPGEKGEKEGGEGQWTSSTSVEQARERSYHRALLLSKDKDTDGETEGGDTPALRDCPSPSLQKTPTHTPCSPGPLAQSTNRPPKEEDGDGQPSKPAGPKSAPLTPTKLHPTPLPSSPVHYPGPSLLHSPKPQPQGSPYRSQRALFSAQPQNQPQPQAQTGPAPFPQYNTQSAPPPPPPPPPAPPASTAYFPSQSPSPAGPFPGFKPAVTPPYPPCSQPLMQTLPHSVHYQSSAAPPPPPPPPPHPMPGPTLLHVNLQPPPIQQHQLMLTTAPPPPPPPQGQTSQQQQQQPPAGSSLLSLTPPPPPPPPPPAPSTNAPMQAHHFQNLGGFQPALLHPGGAATNPSVPQSTYPPPLQQTGLPPPPPPPPQQTQQGQAQAAASQMPSGTRGAPASSTPFHSSGYLSTGWH

Target ORF information:

RefSeq Version	XM_019278449.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019278449.2

ORF Insert Sequence:

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACAGCAGACA CCTCATACCT GGACATGGCT 
GCGGGCTCAG ACAGACCAGA ATCGGTGGAG GCCAGCCCTG TGGTGGTGGA AAAGTCCAGC 
TACCCACACC AGATCTACAG CAGCAGTTCT CACCATTCCC ACAGTTACAT TGGCCTGCCT 
TACGCTGACC ATAACTATGG GGCGCGGCCC CCACCTACAC CACCAGCCTC CCCTCCCCCC 
TCCATGTTGA TCCGACAAGG AGAGGGGGGG TTGTTTGTTC CAGGGGGCCA GGACGAAGCA 
TCAAGGGGCA CGACGCTCAG CACCTCAGAG GATGGCAGCT ACGGGGCTGA CATCACCCGC 
TGCATCTGTG GCTTCACCCA CGATGACGGC TACATGATCT GCTGTGATAA GTGCAGTGCC 
TGGCAGCATA TAGACTGCAT GGGCATCGAC AGGCAGAACA TTCCTGAGAC CTACCTGTGT 
GAACGCTGCC AACCACGACA CCTGGATCGA GAGAGGGCCA TCCTGCTGCA GACCAGGAAA 
CGGGAATGTT TGTCTGATGG GGACACGAGT GCTACAGAGA GTGGAGATGA GGTGCCGCTA 
GAGCTCTACT CCACCTTCCA ACACACGCCT ACCACCATCA CCCTGACTAC CGCGCGCCTT 
GGCAATAAGC AGGCTGATAA GAAACGCAAA AAGAGCGGTG ATAAAGAGCC TCCAGCGTCT 
TCTGCCCGAG CAAAGAAGGC CTTCCGAGAA GGGTCCAGAA AGTCCTCCAG AGTAAAGGGT 
GCAGCGCCAG AGCAGGAGCC AACAGAGTCC CTGTGGGAGA ACAAGATCAA GACGTGGATG 
GAGCGTTATG AGGAGGCCAG CAGCAATCAG TACAGCGAGG ATGTCCAGGT CCTGTTGCGT 
GTCAAAGAAC AAGGCGATGG CAAGAGCCTG GCGTACAACA CGCACCCTGC CTCTTTCAAA 
CCCCCTGTGG AGAGTCAAGT TCAGAAGAAC AAGAAGATCC TCAAAGCGGT GCGAGACTTG 
GCCCCAGACT CCCTCATCAT TGAGTACAGG GGCAAGTTCA TGCTCAGACA GCAGTTTGAG 
GCCAACGGAT ACTTCTTTAA GAGGCCATAC CCATTTGTGT TATTTTATTC TAAATTTGAC 
GGACTGGAGA TGTGCGTGGA CGCTCGTAGC TTCGGCAACG AAGCGCGCTT CATCCGTCGC 
TCCTGCACCC CTAATTCTGA AGTGCGGCAC GTCATTGAGG ACGGCATGCT GCATTTATAC 
ATCTACTCAT TGAGGCCGAT CACCAAGGGC ACAGAGATCA CCATCGGTTT TGATTTTGAC 
TATGGCAGCT GTAAATACAA GGTGGACTGT GCCTGCGTGA AGGGGAATCA GGAGTGCCCG 
GTGCTCAAGC ACAACCTCGA ACCCACAGAG AACCTGGTCG GCCGGCGCAG AGGGAGTCGT 
AAGGACAAGG AGACGGTTCG AGACGACCTG GGCCAGAACC AGAACATGGG TCTGGACGGC 
GAGGGGAAGG GCAAGAGCGT GGGCGACGGC AAACAGAGGA AGCTCTCTCC TCTTCGCCTC 
TCTATCTCAA ACAACCAGGC CAACCCAGCG GAGCAGTCCC ATCTCCCTGT CGGGGCGGCT 
TCCAGCTGGA AAGAACTGAA ACACAAGGAG GTAAACTTTA CTCCACCCAT CTACCAGCCT 
CCCCACCACC ACCTCTCCCC CAAATCTCCC AGAGAGACAC GAGAGGAGAG GAAGATGGAG 
GCCATCCTGC AAGCGTTTGC CCGAATGGAG AAGAGAGAGA AGCGAAGGGA GCAGGCGCTG 
GAAAGAATCG GTAGCATCAA GTCGGAGGTC GGGGGACGCA GTGACATCAA GGAGGAGCCT 
CCAGCCACGC CAGAGACGGC AGATTCTCCA GCTGTTATGC AGCCGCTCCT GGAGGTGAAA 
GAGGAGCCAG GATTAAAGCC GGCCAAGGTC AAAGGCTCGA GGAACAGGAA AAGTTTCTCA 
AGGAACCGCA CGCACATTGG CCAACAGCGC CGGAGAGCTC GCACCATCAG CACCTGCTCT 
GACCTGCAGC CTGGCTCTCC AATTGAATCT GTGGAGCCTC TGAGCAACGA AGCCCCCGAA 
GGAGAGACAC CCACCGCACC AGAGCCGGAG GCAATCTCTT CCCAAGCACC AGACACCAGC 
CCTCCGCACA GCAGCTCACC TGCACCTGAC AGAAACCGCA CCGGGAGCAA GAACTTCAAA 
ACTAAAAAGC ACTTTGTGAG TGAGTGGGTG GGAGAGAAGC AGCAGGACCG TGGTGCAGTA 
CGAACCCCAG AGCCGGCCCC CGAGAGGCCG CTGAGAATAA GCAGTGACCC CGAAGTACTC 
GCCACACAGC TGAACGCCCT CCCAGGCATG GCCTGCTCTC CACAGGTCTA CAGCACGCCC 
AAACACTACG TCCGCTTCTC GTCCCCTTTC CTGGCGAACC GCAGTCCGAC AACTCCCGGA 
GTCCCCACTG GGAGACGGCG TTCCAGAGAA CTGCCAGAAA CGCCGCCAAC CACCGGCTCC 
TGCAAGAAGC GATGGTTGAA GCAGGCCCTT GAGGAAGAGG GATCCACCAG CCCAGCCAGA 
CGACCAAGCC TCCTCATGCC CTGTGAGGGT CCTCTTAGCC CCTCAATCAA CGGAGACTCT 
GACAGCCCTC TTCCCTACAA TGGCACCTGC TCGCTACCTG AGTTGCCCAC GCCTTTGAAA 
AAGCGCCGGT TGAGTCCATT AGATGCCTGT ATGTCTGAGA GCTCCACACC CTACGGCTCC 
CCTTGTGCCA CGCCCACCAG GGCAGACCAA TCGGAGACAC CCGCAACCCC CATCTTACTG 
GCTACCCCAC CGCGTCCCAG AACGGAGGAG CCCAGTACAG AGCCCCCGCC CAACACCCCA 
ACGCAGACAC TTAACGCCCC TCTGGAGAGT GAATCCTCTG TGGAGAGCTC CCCAGAGCTC 
AGCCGGAAAC CCAGTGTACA AGAGGCCGAT CGTCCTCCTT CGTTGGTCTC CTCTCCCTGC 
ATCAGGGCTC CCAGTTCAGA CGGACTCCCG GCTGAAGCCA AAGTGTCGGT TCCTGAGAGT 
CCACAGCCTC CGGTCGCCGA CTCTGTGGAC TGTGGAGAGG ACAGGACCGA TGGTGGGGTT 
GTAGAAGGCA GTAGTGAGCC TTCCTCGTCC GCAGAAACAT GTGCTTCCTC TTTTCCTGGC 
TGGATAAAAA GCCCAGACAG AGGCCCGACT GGACCAGCTG GCCTGAACTT CTCTCCAGTC 
AACTCAAACC TAAGGGACCT CACCCCCTCA CACACTCTGG AGCCCCTGGC GGCTCCCTTC 
AGGCCCGACG CTGCAGCTGG GGCTGCGGCA GGGTCCACAG CAGGGACCGG GTCATTGGTT 
GGCCCTCAAG CCCCCTTCAG TGAAGGCCAG GGGCAGCTGT TTTACCCCTG CTCTGAGGAG 
GGAGGTTCAC TCGGCTTCTC GCGCTCACTG AATGGGGATG GCAGCGGTGA AGGAGGAGGA 
TCAGCGCAGA ACCCCCCACA GAAGAAAAAG GTTTCCCTGC TAGAATACAG GAAACGTCAG 
CGCGAAGCCC GTCGCAGCGG CTCCAAGACC GAGTGCAGCT CTCCTGTCTC CACAGTGCCA 
CCTCTGACCG TGGACGCCTT CCCCGTCGCG CTGGAGACGA CAAGCGAACC TCCACTACCC 
CCTGCTCCTA CAGCTGTCTG CAACGCCAAC AACACCATCG TTGTTAAAGA GCCCCAGACG 
AGCGAGGAGG CCGAGACGCC CGGAGAGAAA GGCGAGAAGG AAGGAGGAGA AGGACAGTGG 
ACGTCGTCCA CTTCTGTAGA GCAGGCCCGA GAACGCAGCT ACCACAGAGC TCTGCTGCTC 
AGCAAAGACA AGGACACGGA TGGTGAGACA GAAGGTGGAG ATACACCTGC ACTGAGAGAT 
TGCCCGTCTC CAAGTCTTCA GAAGACCCCG ACTCACACCC CCTGCTCTCC TGGTCCTCTC 
GCTCAGTCCA CCAATCGGCC ACCGAAGGAG GAAGACGGCG ACGGGCAGCC GAGCAAGCCT 
GCGGGACCCA AGTCGGCTCC TCTGACCCCC ACAAAGCTGC ATCCCACCCC GTTACCCTCC 
TCGCCGGTCC ACTATCCGGG ACCCTCCCTC CTGCACTCGC CCAAGCCTCA GCCTCAGGGC 
TCGCCGTACC GCAGCCAGAG GGCGCTGTTC TCCGCTCAGC CTCAAAACCA ACCGCAGCCC 
CAGGCTCAGA CTGGCCCAGC TCCTTTCCCC CAGTATAACA CACAGAGTGC TCCACCGCCA 
CCTCCCCCTC CTCCACCAGC ACCACCGGCC TCCACGGCGT ATTTTCCCAG CCAGAGCCCC 
TCGCCTGCTG GACCTTTCCC CGGGTTCAAA CCCGCTGTCA CACCCCCTTA CCCTCCGTGT 
TCTCAGCCCC TGATGCAGAC TCTTCCCCAC AGCGTGCACT ATCAGAGCTC AGCCGCTCCA 
CCGCCGCCGC CTCCTCCGCC CCCGCACCCG ATGCCCGGCC CCACCCTGCT GCACGTCAAC 
CTGCAGCCCC CTCCCATCCA GCAGCACCAG CTCATGCTGA CCACCGCCCC TCCGCCGCCT 
CCTCCCCCAC AGGGCCAGAC CTCACAGCAG CAGCAGCAGC AGCCTCCCGC TGGCAGCTCC 
CTGCTGTCGC TCACCCCTCC TCCGCCGCCT CCTCCACCTC CTCCAGCCCC CTCGACCAAC 
GCCCCGATGC AGGCCCACCA CTTTCAGAAC TTAGGGGGTT TTCAGCCGGC GTTGCTACAC 
CCGGGCGGCG CCGCCACAAA CCCTTCAGTG CCCCAATCCA CGTACCCCCC ACCCCTTCAG 
CAGACCGGAC TGCCTCCACC TCCCCCTCCT CCTCCCCAAC AGACTCAACA AGGCCAGGCT 
CAGGCCGCCG CCTCCCAGAT GCCTTCTGGG ACTCGTGGAG CTCCTGCGTC CTCGACCCCC 
TTCCACAGCT CGGGGTACCT GAGCACAGGG TGGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kmt2e ( XM_010738912.3 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010737214.3 Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X5, mRNA.

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CloneID	OLa211867
Clone ID Related Accession (Same CDS sequence)	XM_010738912.3
Accession Version	XM_010738912.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	inactive histone-lysine N-methyltransferase 2E isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040030.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_010738912.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACAGCAGACA CCTCATACCT GGACATGGCT 
GCGGGCTCAG ACAGACCAGA ATCGGTGGAG GCCAGCCCTG TGGTGGTGGA AAAGTCCAGC 
TACCCACACC AGATCTACAG CAGCAGTTCT CACCATTCCC ACAGTTACAT TGGCCTGCCT 
TACGCTGACC ATAACTATGG GGCGCGGCCC CCACCTACAC CACCAGCCTC CCCTCCCCCC 
TCCATGTTGA TCCGACAAGG AGAGGGGGGG TTGTTTGTTC CAGGGGGCCA GGACGAAGCA 
TCAAGGGGCA CGACGCTCAG CACCTCAGAG GATGGCAGCT ACGGGGCTGA CATCACCCGC 
TGCATCTGTG GCTTCACCCA CGATGACGGC TACATGATCT GCTGTGATAA GTGCAGTGCC 
TGGCAGCATA TAGACTGCAT GGGCATCGAC AGGCAGAACA TTCCTGAGAC CTACCTGTGT 
GAACGCTGCC AACCACGACA CCTGGATCGA GAGAGGGCCA TCCTGCTGCA GACCAGGAAA 
CGGGAATGTT TGTCTGATGG GGACACGAGT GCTACAGAGA GTGGAGATGA GGTGCCGCTA 
GAGCTCTACT CCACCTTCCA ACACACGCCT ACCACCATCA CCCTGACTAC CGCGCGCCTT 
GGCAATAAGC AGGCTGATAA GAAACGCAAA AAGAGCGGTG ATAAAGAGCC TCCAGCGTCT 
TCTGCCCGAG CAAAGAAGGC CTTCCGAGAA GGGTCCAGAA AGTCCTCCAG AGTAAAGGGT 
GCAGCGCCAG AGCAGGAGCC AACAGAGTCC CTGTGGGAGA ACAAGATCAA GACGTGGATG 
GAGCGTTATG AGGAGGCCAG CAGCAATCAG TACAGCGAGG ATGTCCAGGT CCTGTTGCGT 
GTCAAAGAAC AAGGCGATGG CAAGAGCCTG GCGTACAACA CGCACCCTGC CTCTTTCAAA 
CCCCCTGTGG AGAGTCAAGT TCAGAAGAAC AAGAAGATCC TCAAAGCGGT GCGAGACTTG 
GCCCCAGACT CCCTCATCAT TGAGTACAGG GGCAAGTTCA TGCTCAGACA GCAGTTTGAG 
GCCAACGGAT ACTTCTTTAA GAGGCCATAC CCATTTGTGT TATTTTATTC TAAATTTGAC 
GGACTGGAGA TGTGCGTGGA CGCTCGTAGC TTCGGCAACG AAGCGCGCTT CATCCGTCGC 
TCCTGCACCC CTAATTCTGA AGTGCGGCAC GTCATTGAGG ACGGCATGCT GCATTTATAC 
ATCTACTCAT TGAGGCCGAT CACCAAGGGC ACAGAGATCA CCATCGGTTT TGATTTTGAC 
TATGGCAGCT GTAAATACAA GGTGGACTGT GCCTGCGTGA AGGGGAATCA GGAGTGCCCG 
GTGCTCAAGC ACAACCTCGA ACCCACAGAG AACCTGGTCG GCCGGCGCAG AGGGAGTCGT 
AAGGACAAGG AGACGGTTCG AGACGACCTG GGCCAGAACC AGAACATGGG TCTGGACGGC 
GAGGGGAAGG GCAAGAGCGT GGGCGACGGC AAACAGAGGA AGCTCTCTCC TCTTCGCCTC 
TCTATCTCAA ACAACCAGGA TCCTGAGTTA TATGAGGATC TAGAAGACAA AACCTCCGTT 
AGCAATGAAG TAGAGATGGA GTCAGAGGAG CAGATTGCAG AGAGGAGGAG GAAGATGACA 
CGAGAGGAGA GGAAGATGGA GGCCATCCTG CAAGCGTTTG CCCGAATGGA GAAGAGAGAG 
AAGCGAAGGG AGCAGGCGCT GGAAAGAATC GGTAGCATCA AGTCGGAGGT CGGGGGACGC 
AGTGACATCA AGGAGGAGCC TCCAGCCACG CCAGAGACGG CAGATTCTCC AGCTGTTATG 
CAGCCGCTCC TGGAGGTGAA AGAGGAGCCA GGATTAAAGC CGGCCAAGGT CAAAGGCTCG 
AGGAACAGGA AAAGTTTCTC AAGGAACCGC ACGCACATTG GCCAACAGCG CCGGAGAGCT 
CGCACCATCA GCACCTGCTC TGACCTGCAG CCTGGCTCTC CAATTGAATC TGTGGAGCCT 
CTGAGCAACG AAGCCCCCGA AGGAGAGACA CCCACCGCAC CAGAGCCGGA GGCAATCTCT 
TCCCAAGCAC CAGACACCAG CCCTCCGCAC AGCAGCTCAC CTGCACCTGA CAGAAACCGC 
ACCGGGAGCA AGAACTTCAA AACTAAAAAG CACTTTGTGA GTGAGTGGGT GGGAGAGAAG 
CAGCAGGACC GTGGTGCAGT ACGAACCCCA GAGCCGGCCC CCGAGAGGCC GCTGAGAATA 
AGCAGTGACC CCGAAGTACT CGCCACACAG CTGAACGCCC TCCCAGGCAT GGCCTGCTCT 
CCACAGGTCT ACAGCACGCC CAAACACTAC GTCCGCTTCT CGTCCCCTTT CCTGGCGAAC 
CGCAGTCCGA CAACTCCCGG AGTCCCCACT GGGAGACGGC GTTCCAGAGA ACTGCCAGAA 
ACGCCGCCAA CCACCGGCTC CTGCAAGAAG CGATGGTTGA AGCAGGCCCT TGAGGAAGAG 
GGATCCACCA GCCCAGCCAG ACGACCAAGC CTCCTCATGC CCTGTGAGGG TCCTCTTAGC 
CCCTCAATCA ACGGAGACTC TGACAGCCCT CTTCCCTACA ATGGCACCTG CTCGCTACCT 
GAGTTGCCCA CGCCTTTGAA AAAGCGCCGG TTGAGTCCAT TAGATGCCTG TATGTCTGAG 
AGCTCCACAC CCTACGGCTC CCCTTGTGCC ACGCCCACCA GGGCAGACCA ATCGGAGACA 
CCCGCAACCC CCATCTTACT GGCTACCCCA CCGCGTCCCA GAACGGAGGA GCCCAGTACA 
GAGCCCCCGC CCAACACCCC AACGCAGACA CTTAACGCCC CTCTGGAGAG TGAATCCTCT 
GTGGAGAGCT CCCCAGAGCT CAGCCGGAAA CCCAGTGTAC AAGAGGCCGA TCGTCCTCCT 
TCGTTGGTCT CCTCTCCCTG CATCAGGGCT CCCAGTTCAG ACGGACTCCC GGCTGAAGCC 
AAAGTGTCGG TTCCTGAGAG TCCACAGCCT CCGGTCGCCG ACTCTGTGGA CTGTGGAGAG 
GACAGGACCG ATGGTGGGGT TGTAGAAGGC AGTAGTGAGC CTTCCTCGTC CGCAGAAACA 
TGTGCTTCCT CTTTTCCTGG CTGGATAAAA AGCCCAGACA GAGGCCCGAC TGGACCAGCT 
GGCCTGAACT TCTCTCCAGT CAACTCAAAC CTAAGGGACC TCACCCCCTC ACACACTCTG 
GAGCCCCTGG CGGCTCCCTT CAGGCCCGAC GCTGCAGCTG GGGCTGCGGC AGGGTCCACA 
GCAGGGACCG GGTCATTGGT TGGCCCTCAA GCCCCCTTCA GTGAAGGCCA GGGGCAGCTG 
TTTTACCCCT GCTCTGAGGA GGGAGGTTCA CTCGGCTTCT CGCGCTCACT GAATGGGGAT 
GGCAGCGGTG AAGGAGGAGG ATCAGCGCAG AACCCCCCAC AGAAGAAAAA GGTTTCCCTG 
CTAGAATACA GGAAACGTCA GCGCGAAGCC CGTCGCAGCG GCTCCAAGAC CGAGTGCAGC 
TCTCCTGTCT CCACAGTGCC ACCTCTGACC GTGGACGCCT TCCCCGTCGC GCTGGAGACG 
ACAAGCGAAC CTCCACTACC CCCTGCTCCT ACAGCTGTCT GCAACGCCAA CAACACCATC 
GTTGTTAAAG AGCCCCAGAC GAGCGAGGAG GCCGAGACGC CCGGAGAGAA AGGCGAGAAG 
GAAGGAGGAG AAGGACAGTG GACGTCGTCC ACTTCTGTAG AGCAGGCCCG AGAACGCAGC 
TACCACAGAG CTCTGCTGCT CAGCAAAGAC AAGGACACGG ATGGTGAGAC AGAAGGTGGA 
GATACACCTG CACTGAGAGA TTGCCCGTCT CCAAGTCTTC AGAAGACCCC GACTCACACC 
CCCTGCTCTC CTGGTCCTCT CGCTCAGTCC ACCAATCGGC CACCGAAGGA GGAAGACGGC 
GACGGGCAGC CGAGCAAGCC TGCGGGACCC AAGTCGGCTC CTCTGACCCC CACAAAGCTG 
CATCCCACCC CGTTACCCTC CTCGCCGGTC CACTATCCGG GACCCTCCCT CCTGCACTCG 
CCCAAGCCTC AGCCTCAGGG CTCGCCGTAC CGCAGCCAGA GGGCGCTGTT CTCCGCTCAG 
CCTCAAAACC AACCGCAGCC CCAGGCTCAG ACTGGCCCAG CTCCTTTCCC CCAGTATAAC 
ACACAGAGTG CTCCACCGCC ACCTCCCCCT CCTCCACCAG CACCACCGGC CTCCACGGCG 
TATTTTCCCA GCCAGAGCCC CTCGCCTGCT GGACCTTTCC CCGGGTTCAA ACCCGCTGTC 
ACACCCCCTT ACCCTCCGTG TTCTCAGCCC CTGATGCAGA CTCTTCCCCA CAGCGTGCAC 
TATCAGAGCT CAGCCGCTCC ACCGCCGCCG CCTCCTCCGC CCCCGCACCC GATGCCCGGC 
CCCACCCTGC TGCACGTCAA CCTGCAGCCC CCTCCCATCC AGCAGCACCA GCTCATGCTG 
ACCACCGCCC CTCCGCCGCC TCCTCCCCCA CAGGGCCAGA CCTCACAGCA GCAGCAGCAG 
CAGCCTCCCG CTGGCAGCTC CCTGCTGTCG CTCACCCCTC CTCCGCCGCC TCCTCCACCT 
CCTCCAGCCC CCTCGACCAA CGCCCCGATG CAGGCCCACC ACTTTCAGAA CTTAGGGGGT 
TTTCAGCCGG CGTTGCTACA CCCGGGCGGC GCCGCCACAA ACCCTTCAGT GCCCCAATCC 
ACGTACCCCC CACCCCTTCA GCAGACCGGA CTGCCTCCAC CTCCCCCTCC TCCTCCCCAA 
CAGACTCAAC AAGGCCAGGC TCAGGCCGCC GCCTCCCAGA TGCCTTCTGG GACTCGTGGA 
GCTCCTGCGT CCTCGACCCC CTTCCACAGC TCGGGGTACC TGAGCACAGG GTGGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010737214.3
CDS	483..5402
Translation	MSIVIPVGVDTADTSYLDMAAGSDRPESVEASPVVVEKSSYPHQIYSSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPSMLIRQGEGGLFVPGGQDEASRGTTLSTSEDGSYGADITRCICGFTHDDGYMICCDKCSAWQHIDCMGIDRQNIPETYLCERCQPRHLDRERAILLQTRKRECLSDGDTSATESGDEVPLELYSTFQHTPTTITLTTARLGNKQADKKRKKSGDKEPPASSARAKKAFREGSRKSSRVKGAAPEQEPTESLWENKIKTWMERYEEASSNQYSEDVQVLLRVKEQGDGKSLAYNTHPASFKPPVESQVQKNKKILKAVRDLAPDSLIIEYRGKFMLRQQFEANGYFFKRPYPFVLFYSKFDGLEMCVDARSFGNEARFIRRSCTPNSEVRHVIEDGMLHLYIYSLRPITKGTEITIGFDFDYGSCKYKVDCACVKGNQECPVLKHNLEPTENLVGRRRGSRKDKETVRDDLGQNQNMGLDGEGKGKSVGDGKQRKLSPLRLSISNNQDPELYEDLEDKTSVSNEVEMESEEQIAERRRKMTREERKMEAILQAFARMEKREKRREQALERIGSIKSEVGGRSDIKEEPPATPETADSPAVMQPLLEVKEEPGLKPAKVKGSRNRKSFSRNRTHIGQQRRRARTISTCSDLQPGSPIESVEPLSNEAPEGETPTAPEPEAISSQAPDTSPPHSSSPAPDRNRTGSKNFKTKKHFVSEWVGEKQQDRGAVRTPEPAPERPLRISSDPEVLATQLNALPGMACSPQVYSTPKHYVRFSSPFLANRSPTTPGVPTGRRRSRELPETPPTTGSCKKRWLKQALEEEGSTSPARRPSLLMPCEGPLSPSINGDSDSPLPYNGTCSLPELPTPLKKRRLSPLDACMSESSTPYGSPCATPTRADQSETPATPILLATPPRPRTEEPSTEPPPNTPTQTLNAPLESESSVESSPELSRKPSVQEADRPPSLVSSPCIRAPSSDGLPAEAKVSVPESPQPPVADSVDCGEDRTDGGVVEGSSEPSSSAETCASSFPGWIKSPDRGPTGPAGLNFSPVNSNLRDLTPSHTLEPLAAPFRPDAAAGAAAGSTAGTGSLVGPQAPFSEGQGQLFYPCSEEGGSLGFSRSLNGDGSGEGGGSAQNPPQKKKVSLLEYRKRQREARRSGSKTECSSPVSTVPPLTVDAFPVALETTSEPPLPPAPTAVCNANNTIVVKEPQTSEEAETPGEKGEKEGGEGQWTSSTSVEQARERSYHRALLLSKDKDTDGETEGGDTPALRDCPSPSLQKTPTHTPCSPGPLAQSTNRPPKEEDGDGQPSKPAGPKSAPLTPTKLHPTPLPSSPVHYPGPSLLHSPKPQPQGSPYRSQRALFSAQPQNQPQPQAQTGPAPFPQYNTQSAPPPPPPPPPAPPASTAYFPSQSPSPAGPFPGFKPAVTPPYPPCSQPLMQTLPHSVHYQSSAAPPPPPPPPPHPMPGPTLLHVNLQPPPIQQHQLMLTTAPPPPPPPQGQTSQQQQQQPPAGSSLLSLTPPPPPPPPPPAPSTNAPMQAHHFQNLGGFQPALLHPGGAATNPSVPQSTYPPPLQQTGLPPPPPPPPQQTQQGQAQAAASQMPSGTRGAPASSTPFHSSGYLSTGWH

Target ORF information:

RefSeq Version	XM_010738912.3
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010738912.3

ORF Insert Sequence:

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACAGCAGACA CCTCATACCT GGACATGGCT 
GCGGGCTCAG ACAGACCAGA ATCGGTGGAG GCCAGCCCTG TGGTGGTGGA AAAGTCCAGC 
TACCCACACC AGATCTACAG CAGCAGTTCT CACCATTCCC ACAGTTACAT TGGCCTGCCT 
TACGCTGACC ATAACTATGG GGCGCGGCCC CCACCTACAC CACCAGCCTC CCCTCCCCCC 
TCCATGTTGA TCCGACAAGG AGAGGGGGGG TTGTTTGTTC CAGGGGGCCA GGACGAAGCA 
TCAAGGGGCA CGACGCTCAG CACCTCAGAG GATGGCAGCT ACGGGGCTGA CATCACCCGC 
TGCATCTGTG GCTTCACCCA CGATGACGGC TACATGATCT GCTGTGATAA GTGCAGTGCC 
TGGCAGCATA TAGACTGCAT GGGCATCGAC AGGCAGAACA TTCCTGAGAC CTACCTGTGT 
GAACGCTGCC AACCACGACA CCTGGATCGA GAGAGGGCCA TCCTGCTGCA GACCAGGAAA 
CGGGAATGTT TGTCTGATGG GGACACGAGT GCTACAGAGA GTGGAGATGA GGTGCCGCTA 
GAGCTCTACT CCACCTTCCA ACACACGCCT ACCACCATCA CCCTGACTAC CGCGCGCCTT 
GGCAATAAGC AGGCTGATAA GAAACGCAAA AAGAGCGGTG ATAAAGAGCC TCCAGCGTCT 
TCTGCCCGAG CAAAGAAGGC CTTCCGAGAA GGGTCCAGAA AGTCCTCCAG AGTAAAGGGT 
GCAGCGCCAG AGCAGGAGCC AACAGAGTCC CTGTGGGAGA ACAAGATCAA GACGTGGATG 
GAGCGTTATG AGGAGGCCAG CAGCAATCAG TACAGCGAGG ATGTCCAGGT CCTGTTGCGT 
GTCAAAGAAC AAGGCGATGG CAAGAGCCTG GCGTACAACA CGCACCCTGC CTCTTTCAAA 
CCCCCTGTGG AGAGTCAAGT TCAGAAGAAC AAGAAGATCC TCAAAGCGGT GCGAGACTTG 
GCCCCAGACT CCCTCATCAT TGAGTACAGG GGCAAGTTCA TGCTCAGACA GCAGTTTGAG 
GCCAACGGAT ACTTCTTTAA GAGGCCATAC CCATTTGTGT TATTTTATTC TAAATTTGAC 
GGACTGGAGA TGTGCGTGGA CGCTCGTAGC TTCGGCAACG AAGCGCGCTT CATCCGTCGC 
TCCTGCACCC CTAATTCTGA AGTGCGGCAC GTCATTGAGG ACGGCATGCT GCATTTATAC 
ATCTACTCAT TGAGGCCGAT CACCAAGGGC ACAGAGATCA CCATCGGTTT TGATTTTGAC 
TATGGCAGCT GTAAATACAA GGTGGACTGT GCCTGCGTGA AGGGGAATCA GGAGTGCCCG 
GTGCTCAAGC ACAACCTCGA ACCCACAGAG AACCTGGTCG GCCGGCGCAG AGGGAGTCGT 
AAGGACAAGG AGACGGTTCG AGACGACCTG GGCCAGAACC AGAACATGGG TCTGGACGGC 
GAGGGGAAGG GCAAGAGCGT GGGCGACGGC AAACAGAGGA AGCTCTCTCC TCTTCGCCTC 
TCTATCTCAA ACAACCAGGA TCCTGAGTTA TATGAGGATC TAGAAGACAA AACCTCCGTT 
AGCAATGAAG TAGAGATGGA GTCAGAGGAG CAGATTGCAG AGAGGAGGAG GAAGATGACA 
CGAGAGGAGA GGAAGATGGA GGCCATCCTG CAAGCGTTTG CCCGAATGGA GAAGAGAGAG 
AAGCGAAGGG AGCAGGCGCT GGAAAGAATC GGTAGCATCA AGTCGGAGGT CGGGGGACGC 
AGTGACATCA AGGAGGAGCC TCCAGCCACG CCAGAGACGG CAGATTCTCC AGCTGTTATG 
CAGCCGCTCC TGGAGGTGAA AGAGGAGCCA GGATTAAAGC CGGCCAAGGT CAAAGGCTCG 
AGGAACAGGA AAAGTTTCTC AAGGAACCGC ACGCACATTG GCCAACAGCG CCGGAGAGCT 
CGCACCATCA GCACCTGCTC TGACCTGCAG CCTGGCTCTC CAATTGAATC TGTGGAGCCT 
CTGAGCAACG AAGCCCCCGA AGGAGAGACA CCCACCGCAC CAGAGCCGGA GGCAATCTCT 
TCCCAAGCAC CAGACACCAG CCCTCCGCAC AGCAGCTCAC CTGCACCTGA CAGAAACCGC 
ACCGGGAGCA AGAACTTCAA AACTAAAAAG CACTTTGTGA GTGAGTGGGT GGGAGAGAAG 
CAGCAGGACC GTGGTGCAGT ACGAACCCCA GAGCCGGCCC CCGAGAGGCC GCTGAGAATA 
AGCAGTGACC CCGAAGTACT CGCCACACAG CTGAACGCCC TCCCAGGCAT GGCCTGCTCT 
CCACAGGTCT ACAGCACGCC CAAACACTAC GTCCGCTTCT CGTCCCCTTT CCTGGCGAAC 
CGCAGTCCGA CAACTCCCGG AGTCCCCACT GGGAGACGGC GTTCCAGAGA ACTGCCAGAA 
ACGCCGCCAA CCACCGGCTC CTGCAAGAAG CGATGGTTGA AGCAGGCCCT TGAGGAAGAG 
GGATCCACCA GCCCAGCCAG ACGACCAAGC CTCCTCATGC CCTGTGAGGG TCCTCTTAGC 
CCCTCAATCA ACGGAGACTC TGACAGCCCT CTTCCCTACA ATGGCACCTG CTCGCTACCT 
GAGTTGCCCA CGCCTTTGAA AAAGCGCCGG TTGAGTCCAT TAGATGCCTG TATGTCTGAG 
AGCTCCACAC CCTACGGCTC CCCTTGTGCC ACGCCCACCA GGGCAGACCA ATCGGAGACA 
CCCGCAACCC CCATCTTACT GGCTACCCCA CCGCGTCCCA GAACGGAGGA GCCCAGTACA 
GAGCCCCCGC CCAACACCCC AACGCAGACA CTTAACGCCC CTCTGGAGAG TGAATCCTCT 
GTGGAGAGCT CCCCAGAGCT CAGCCGGAAA CCCAGTGTAC AAGAGGCCGA TCGTCCTCCT 
TCGTTGGTCT CCTCTCCCTG CATCAGGGCT CCCAGTTCAG ACGGACTCCC GGCTGAAGCC 
AAAGTGTCGG TTCCTGAGAG TCCACAGCCT CCGGTCGCCG ACTCTGTGGA CTGTGGAGAG 
GACAGGACCG ATGGTGGGGT TGTAGAAGGC AGTAGTGAGC CTTCCTCGTC CGCAGAAACA 
TGTGCTTCCT CTTTTCCTGG CTGGATAAAA AGCCCAGACA GAGGCCCGAC TGGACCAGCT 
GGCCTGAACT TCTCTCCAGT CAACTCAAAC CTAAGGGACC TCACCCCCTC ACACACTCTG 
GAGCCCCTGG CGGCTCCCTT CAGGCCCGAC GCTGCAGCTG GGGCTGCGGC AGGGTCCACA 
GCAGGGACCG GGTCATTGGT TGGCCCTCAA GCCCCCTTCA GTGAAGGCCA GGGGCAGCTG 
TTTTACCCCT GCTCTGAGGA GGGAGGTTCA CTCGGCTTCT CGCGCTCACT GAATGGGGAT 
GGCAGCGGTG AAGGAGGAGG ATCAGCGCAG AACCCCCCAC AGAAGAAAAA GGTTTCCCTG 
CTAGAATACA GGAAACGTCA GCGCGAAGCC CGTCGCAGCG GCTCCAAGAC CGAGTGCAGC 
TCTCCTGTCT CCACAGTGCC ACCTCTGACC GTGGACGCCT TCCCCGTCGC GCTGGAGACG 
ACAAGCGAAC CTCCACTACC CCCTGCTCCT ACAGCTGTCT GCAACGCCAA CAACACCATC 
GTTGTTAAAG AGCCCCAGAC GAGCGAGGAG GCCGAGACGC CCGGAGAGAA AGGCGAGAAG 
GAAGGAGGAG AAGGACAGTG GACGTCGTCC ACTTCTGTAG AGCAGGCCCG AGAACGCAGC 
TACCACAGAG CTCTGCTGCT CAGCAAAGAC AAGGACACGG ATGGTGAGAC AGAAGGTGGA 
GATACACCTG CACTGAGAGA TTGCCCGTCT CCAAGTCTTC AGAAGACCCC GACTCACACC 
CCCTGCTCTC CTGGTCCTCT CGCTCAGTCC ACCAATCGGC CACCGAAGGA GGAAGACGGC 
GACGGGCAGC CGAGCAAGCC TGCGGGACCC AAGTCGGCTC CTCTGACCCC CACAAAGCTG 
CATCCCACCC CGTTACCCTC CTCGCCGGTC CACTATCCGG GACCCTCCCT CCTGCACTCG 
CCCAAGCCTC AGCCTCAGGG CTCGCCGTAC CGCAGCCAGA GGGCGCTGTT CTCCGCTCAG 
CCTCAAAACC AACCGCAGCC CCAGGCTCAG ACTGGCCCAG CTCCTTTCCC CCAGTATAAC 
ACACAGAGTG CTCCACCGCC ACCTCCCCCT CCTCCACCAG CACCACCGGC CTCCACGGCG 
TATTTTCCCA GCCAGAGCCC CTCGCCTGCT GGACCTTTCC CCGGGTTCAA ACCCGCTGTC 
ACACCCCCTT ACCCTCCGTG TTCTCAGCCC CTGATGCAGA CTCTTCCCCA CAGCGTGCAC 
TATCAGAGCT CAGCCGCTCC ACCGCCGCCG CCTCCTCCGC CCCCGCACCC GATGCCCGGC 
CCCACCCTGC TGCACGTCAA CCTGCAGCCC CCTCCCATCC AGCAGCACCA GCTCATGCTG 
ACCACCGCCC CTCCGCCGCC TCCTCCCCCA CAGGGCCAGA CCTCACAGCA GCAGCAGCAG 
CAGCCTCCCG CTGGCAGCTC CCTGCTGTCG CTCACCCCTC CTCCGCCGCC TCCTCCACCT 
CCTCCAGCCC CCTCGACCAA CGCCCCGATG CAGGCCCACC ACTTTCAGAA CTTAGGGGGT 
TTTCAGCCGG CGTTGCTACA CCCGGGCGGC GCCGCCACAA ACCCTTCAGT GCCCCAATCC 
ACGTACCCCC CACCCCTTCA GCAGACCGGA CTGCCTCCAC CTCCCCCTCC TCCTCCCCAA 
CAGACTCAAC AAGGCCAGGC TCAGGCCGCC GCCTCCCAGA TGCCTTCTGG GACTCGTGGA 
GCTCCTGCGT CCTCGACCCC CTTCCACAGC TCGGGGTACC TGAGCACAGG GTGGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kmt2e ( XM_019278450.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019133995.2 Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X6, mRNA.

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CloneID	OLa211868
Clone ID Related Accession (Same CDS sequence)	XM_019278450.2
Accession Version	XM_019278450.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	inactive histone-lysine N-methyltransferase 2E isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040030.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_019278450.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACAGCAGACA CCTCATACCT GGACATGGCT 
GCGGGCTCAG AACCAGAATC GGTGGAGGCC AGCCCTGTGG TGGTGGAAAA GTCCAGCTAC 
CCACACCAGA TCTACAGCAG CAGTTCTCAC CATTCCCACA GTTACATTGG CCTGCCTTAC 
GCTGACCATA ACTATGGGGC GCGGCCCCCA CCTACACCAC CAGCCTCCCC TCCCCCCTCC 
ATGTTGATCC GACAAGGAGA GGGGGGGTTG TTTGTTCCAG GGGGCCAGGA CGAAGCATCA 
AGGGGCACGA CGCTCAGCAC CTCAGAGGAT GGCAGCTACG GGGCTGACAT CACCCGCTGC 
ATCTGTGGCT TCACCCACGA TGACGGCTAC ATGATCTGCT GTGATAAGTG CAGTGCCTGG 
CAGCATATAG ACTGCATGGG CATCGACAGG CAGAACATTC CTGAGACCTA CCTGTGTGAA 
CGCTGCCAAC CACGACACCT GGATCGAGAG AGGGCCATCC TGCTGCAGAC CAGGAAACGG 
GAATGTTTGT CTGATGGGGA CACGAGTGCT ACAGAGAGTG GAGATGAGGT GCCGCTAGAG 
CTCTACTCCA CCTTCCAACA CACGCCTACC ACCATCACCC TGACTACCGC GCGCCTTGGC 
AATAAGCAGG CTGATAAGAA ACGCAAAAAG AGCGGTGATA AAGAGCCTCC AGCGTCTTCT 
GCCCGAGCAA AGAAGGCCTT CCGAGAAGGG TCCAGAAAGT CCTCCAGAGT AAAGGGTGCA 
GCGCCAGAGC AGGAGCCAAC AGAGTCCCTG TGGGAGAACA AGATCAAGAC GTGGATGGAG 
CGTTATGAGG AGGCCAGCAG CAATCAGTAC AGCGAGGATG TCCAGGTCCT GTTGCGTGTC 
AAAGAACAAG GCGATGGCAA GAGCCTGGCG TACAACACGC ACCCTGCCTC TTTCAAACCC 
CCTGTGGAGA GTCAAGTTCA GAAGAACAAG AAGATCCTCA AAGCGGTGCG AGACTTGGCC 
CCAGACTCCC TCATCATTGA GTACAGGGGC AAGTTCATGC TCAGACAGCA GTTTGAGGCC 
AACGGATACT TCTTTAAGAG GCCATACCCA TTTGTGTTAT TTTATTCTAA ATTTGACGGA 
CTGGAGATGT GCGTGGACGC TCGTAGCTTC GGCAACGAAG CGCGCTTCAT CCGTCGCTCC 
TGCACCCCTA ATTCTGAAGT GCGGCACGTC ATTGAGGACG GCATGCTGCA TTTATACATC 
TACTCATTGA GGCCGATCAC CAAGGGCACA GAGATCACCA TCGGTTTTGA TTTTGACTAT 
GGCAGCTGTA AATACAAGGT GGACTGTGCC TGCGTGAAGG GGAATCAGGA GTGCCCGGTG 
CTCAAGCACA ACCTCGAACC CACAGAGAAC CTGGTCGGCC GGCGCAGAGG GAGTCGTAAG 
GACAAGGAGA CGGTTCGAGA CGACCTGGGC CAGAACCAGA ACATGGGTCT GGACGGCGAG 
GGGAAGGGCA AGAGCGTGGG CGACGGCAAA CAGAGGAAGC TCTCTCCTCT TCGCCTCTCT 
ATCTCAAACA ACCAGGATCC TGAGTTATAT GAGGATCTAG AAGACAAAAC CTCCGTTAGC 
AATGAAGTAG AGATGGAGTC AGAGGAGCAG ATTGCAGAGA GGAGGAGGAA GATGACACGA 
GAGGAGAGGA AGATGGAGGC CATCCTGCAA GCGTTTGCCC GAATGGAGAA GAGAGAGAAG 
CGAAGGGAGC AGGCGCTGGA AAGAATCGGT AGCATCAAGT CGGAGGTCGG GGGACGCAGT 
GACATCAAGG AGGAGCCTCC AGCCACGCCA GAGACGGCAG ATTCTCCAGC TGTTATGCAG 
CCGCTCCTGG AGGTGAAAGA GGAGCCAGGA TTAAAGCCGG CCAAGGTCAA AGGCTCGAGG 
AACAGGAAAA GTTTCTCAAG GAACCGCACG CACATTGGCC AACAGCGCCG GAGAGCTCGC 
ACCATCAGCA CCTGCTCTGA CCTGCAGCCT GGCTCTCCAA TTGAATCTGT GGAGCCTCTG 
AGCAACGAAG CCCCCGAAGG AGAGACACCC ACCGCACCAG AGCCGGAGGC AATCTCTTCC 
CAAGCACCAG ACACCAGCCC TCCGCACAGC AGCTCACCTG CACCTGACAG AAACCGCACC 
GGGAGCAAGA ACTTCAAAAC TAAAAAGCAC TTTGTGAGTG AGTGGGTGGG AGAGAAGCAG 
CAGGACCGTG GTGCAGTACG AACCCCAGAG CCGGCCCCCG AGAGGCCGCT GAGAATAAGC 
AGTGACCCCG AAGTACTCGC CACACAGCTG AACGCCCTCC CAGGCATGGC CTGCTCTCCA 
CAGGTCTACA GCACGCCCAA ACACTACGTC CGCTTCTCGT CCCCTTTCCT GGCGAACCGC 
AGTCCGACAA CTCCCGGAGT CCCCACTGGG AGACGGCGTT CCAGAGAACT GCCAGAAACG 
CCGCCAACCA CCGGCTCCTG CAAGAAGCGA TGGTTGAAGC AGGCCCTTGA GGAAGAGGGA 
TCCACCAGCC CAGCCAGACG ACCAAGCCTC CTCATGCCCT GTGAGGGTCC TCTTAGCCCC 
TCAATCAACG GAGACTCTGA CAGCCCTCTT CCCTACAATG GCACCTGCTC GCTACCTGAG 
TTGCCCACGC CTTTGAAAAA GCGCCGGTTG AGTCCATTAG ATGCCTGTAT GTCTGAGAGC 
TCCACACCCT ACGGCTCCCC TTGTGCCACG CCCACCAGGG CAGACCAATC GGAGACACCC 
GCAACCCCCA TCTTACTGGC TACCCCACCG CGTCCCAGAA CGGAGGAGCC CAGTACAGAG 
CCCCCGCCCA ACACCCCAAC GCAGACACTT AACGCCCCTC TGGAGAGTGA ATCCTCTGTG 
GAGAGCTCCC CAGAGCTCAG CCGGAAACCC AGTGTACAAG AGGCCGATCG TCCTCCTTCG 
TTGGTCTCCT CTCCCTGCAT CAGGGCTCCC AGTTCAGACG GACTCCCGGC TGAAGCCAAA 
GTGTCGGTTC CTGAGAGTCC ACAGCCTCCG GTCGCCGACT CTGTGGACTG TGGAGAGGAC 
AGGACCGATG GTGGGGTTGT AGAAGGCAGT AGTGAGCCTT CCTCGTCCGC AGAAACATGT 
GCTTCCTCTT TTCCTGGCTG GATAAAAAGC CCAGACAGAG GCCCGACTGG ACCAGCTGGC 
CTGAACTTCT CTCCAGTCAA CTCAAACCTA AGGGACCTCA CCCCCTCACA CACTCTGGAG 
CCCCTGGCGG CTCCCTTCAG GCCCGACGCT GCAGCTGGGG CTGCGGCAGG GTCCACAGCA 
GGGACCGGGT CATTGGTTGG CCCTCAAGCC CCCTTCAGTG AAGGCCAGGG GCAGCTGTTT 
TACCCCTGCT CTGAGGAGGG AGGTTCACTC GGCTTCTCGC GCTCACTGAA TGGGGATGGC 
AGCGGTGAAG GAGGAGGATC AGCGCAGAAC CCCCCACAGA AGAAAAAGGT TTCCCTGCTA 
GAATACAGGA AACGTCAGCG CGAAGCCCGT CGCAGCGGCT CCAAGACCGA GTGCAGCTCT 
CCTGTCTCCA CAGTGCCACC TCTGACCGTG GACGCCTTCC CCGTCGCGCT GGAGACGACA 
AGCGAACCTC CACTACCCCC TGCTCCTACA GCTGTCTGCA ACGCCAACAA CACCATCGTT 
GTTAAAGAGC CCCAGACGAG CGAGGAGGCC GAGACGCCCG GAGAGAAAGG CGAGAAGGAA 
GGAGGAGAAG GACAGTGGAC GTCGTCCACT TCTGTAGAGC AGGCCCGAGA ACGCAGCTAC 
CACAGAGCTC TGCTGCTCAG CAAAGACAAG GACACGGATG GTGAGACAGA AGGTGGAGAT 
ACACCTGCAC TGAGAGATTG CCCGTCTCCA AGTCTTCAGA AGACCCCGAC TCACACCCCC 
TGCTCTCCTG GTCCTCTCGC TCAGTCCACC AATCGGCCAC CGAAGGAGGA AGACGGCGAC 
GGGCAGCCGA GCAAGCCTGC GGGACCCAAG TCGGCTCCTC TGACCCCCAC AAAGCTGCAT 
CCCACCCCGT TACCCTCCTC GCCGGTCCAC TATCCGGGAC CCTCCCTCCT GCACTCGCCC 
AAGCCTCAGC CTCAGGGCTC GCCGTACCGC AGCCAGAGGG CGCTGTTCTC CGCTCAGCCT 
CAAAACCAAC CGCAGCCCCA GGCTCAGACT GGCCCAGCTC CTTTCCCCCA GTATAACACA 
CAGAGTGCTC CACCGCCACC TCCCCCTCCT CCACCAGCAC CACCGGCCTC CACGGCGTAT 
TTTCCCAGCC AGAGCCCCTC GCCTGCTGGA CCTTTCCCCG GGTTCAAACC CGCTGTCACA 
CCCCCTTACC CTCCGTGTTC TCAGCCCCTG ATGCAGACTC TTCCCCACAG CGTGCACTAT 
CAGAGCTCAG CCGCTCCACC GCCGCCGCCT CCTCCGCCCC CGCACCCGAT GCCCGGCCCC 
ACCCTGCTGC ACGTCAACCT GCAGCCCCCT CCCATCCAGC AGCACCAGCT CATGCTGACC 
ACCGCCCCTC CGCCGCCTCC TCCCCCACAG GGCCAGACCT CACAGCAGCA GCAGCAGCAG 
CCTCCCGCTG GCAGCTCCCT GCTGTCGCTC ACCCCTCCTC CGCCGCCTCC TCCACCTCCT 
CCAGCCCCCT CGACCAACGC CCCGATGCAG GCCCACCACT TTCAGAACTT AGGGGGTTTT 
CAGCCGGCGT TGCTACACCC GGGCGGCGCC GCCACAAACC CTTCAGTGCC CCAATCCACG 
TACCCCCCAC CCCTTCAGCA GACCGGACTG CCTCCACCTC CCCCTCCTCC TCCCCAACAG 
ACTCAACAAG GCCAGGCTCA GGCCGCCGCC TCCCAGATGC CTTCTGGGAC TCGTGGAGCT 
CCTGCGTCCT CGACCCCCTT CCACAGCTCG GGGTACCTGA GCACAGGGTG GCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019133995.2
CDS	483..5399
Translation	MSIVIPVGVDTADTSYLDMAAGSEPESVEASPVVVEKSSYPHQIYSSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPSMLIRQGEGGLFVPGGQDEASRGTTLSTSEDGSYGADITRCICGFTHDDGYMICCDKCSAWQHIDCMGIDRQNIPETYLCERCQPRHLDRERAILLQTRKRECLSDGDTSATESGDEVPLELYSTFQHTPTTITLTTARLGNKQADKKRKKSGDKEPPASSARAKKAFREGSRKSSRVKGAAPEQEPTESLWENKIKTWMERYEEASSNQYSEDVQVLLRVKEQGDGKSLAYNTHPASFKPPVESQVQKNKKILKAVRDLAPDSLIIEYRGKFMLRQQFEANGYFFKRPYPFVLFYSKFDGLEMCVDARSFGNEARFIRRSCTPNSEVRHVIEDGMLHLYIYSLRPITKGTEITIGFDFDYGSCKYKVDCACVKGNQECPVLKHNLEPTENLVGRRRGSRKDKETVRDDLGQNQNMGLDGEGKGKSVGDGKQRKLSPLRLSISNNQDPELYEDLEDKTSVSNEVEMESEEQIAERRRKMTREERKMEAILQAFARMEKREKRREQALERIGSIKSEVGGRSDIKEEPPATPETADSPAVMQPLLEVKEEPGLKPAKVKGSRNRKSFSRNRTHIGQQRRRARTISTCSDLQPGSPIESVEPLSNEAPEGETPTAPEPEAISSQAPDTSPPHSSSPAPDRNRTGSKNFKTKKHFVSEWVGEKQQDRGAVRTPEPAPERPLRISSDPEVLATQLNALPGMACSPQVYSTPKHYVRFSSPFLANRSPTTPGVPTGRRRSRELPETPPTTGSCKKRWLKQALEEEGSTSPARRPSLLMPCEGPLSPSINGDSDSPLPYNGTCSLPELPTPLKKRRLSPLDACMSESSTPYGSPCATPTRADQSETPATPILLATPPRPRTEEPSTEPPPNTPTQTLNAPLESESSVESSPELSRKPSVQEADRPPSLVSSPCIRAPSSDGLPAEAKVSVPESPQPPVADSVDCGEDRTDGGVVEGSSEPSSSAETCASSFPGWIKSPDRGPTGPAGLNFSPVNSNLRDLTPSHTLEPLAAPFRPDAAAGAAAGSTAGTGSLVGPQAPFSEGQGQLFYPCSEEGGSLGFSRSLNGDGSGEGGGSAQNPPQKKKVSLLEYRKRQREARRSGSKTECSSPVSTVPPLTVDAFPVALETTSEPPLPPAPTAVCNANNTIVVKEPQTSEEAETPGEKGEKEGGEGQWTSSTSVEQARERSYHRALLLSKDKDTDGETEGGDTPALRDCPSPSLQKTPTHTPCSPGPLAQSTNRPPKEEDGDGQPSKPAGPKSAPLTPTKLHPTPLPSSPVHYPGPSLLHSPKPQPQGSPYRSQRALFSAQPQNQPQPQAQTGPAPFPQYNTQSAPPPPPPPPPAPPASTAYFPSQSPSPAGPFPGFKPAVTPPYPPCSQPLMQTLPHSVHYQSSAAPPPPPPPPPHPMPGPTLLHVNLQPPPIQQHQLMLTTAPPPPPPPQGQTSQQQQQQPPAGSSLLSLTPPPPPPPPPPAPSTNAPMQAHHFQNLGGFQPALLHPGGAATNPSVPQSTYPPPLQQTGLPPPPPPPPQQTQQGQAQAAASQMPSGTRGAPASSTPFHSSGYLSTGWH

Target ORF information:

RefSeq Version	XM_019278450.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019278450.2

ORF Insert Sequence:

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACAGCAGACA CCTCATACCT GGACATGGCT 
GCGGGCTCAG AACCAGAATC GGTGGAGGCC AGCCCTGTGG TGGTGGAAAA GTCCAGCTAC 
CCACACCAGA TCTACAGCAG CAGTTCTCAC CATTCCCACA GTTACATTGG CCTGCCTTAC 
GCTGACCATA ACTATGGGGC GCGGCCCCCA CCTACACCAC CAGCCTCCCC TCCCCCCTCC 
ATGTTGATCC GACAAGGAGA GGGGGGGTTG TTTGTTCCAG GGGGCCAGGA CGAAGCATCA 
AGGGGCACGA CGCTCAGCAC CTCAGAGGAT GGCAGCTACG GGGCTGACAT CACCCGCTGC 
ATCTGTGGCT TCACCCACGA TGACGGCTAC ATGATCTGCT GTGATAAGTG CAGTGCCTGG 
CAGCATATAG ACTGCATGGG CATCGACAGG CAGAACATTC CTGAGACCTA CCTGTGTGAA 
CGCTGCCAAC CACGACACCT GGATCGAGAG AGGGCCATCC TGCTGCAGAC CAGGAAACGG 
GAATGTTTGT CTGATGGGGA CACGAGTGCT ACAGAGAGTG GAGATGAGGT GCCGCTAGAG 
CTCTACTCCA CCTTCCAACA CACGCCTACC ACCATCACCC TGACTACCGC GCGCCTTGGC 
AATAAGCAGG CTGATAAGAA ACGCAAAAAG AGCGGTGATA AAGAGCCTCC AGCGTCTTCT 
GCCCGAGCAA AGAAGGCCTT CCGAGAAGGG TCCAGAAAGT CCTCCAGAGT AAAGGGTGCA 
GCGCCAGAGC AGGAGCCAAC AGAGTCCCTG TGGGAGAACA AGATCAAGAC GTGGATGGAG 
CGTTATGAGG AGGCCAGCAG CAATCAGTAC AGCGAGGATG TCCAGGTCCT GTTGCGTGTC 
AAAGAACAAG GCGATGGCAA GAGCCTGGCG TACAACACGC ACCCTGCCTC TTTCAAACCC 
CCTGTGGAGA GTCAAGTTCA GAAGAACAAG AAGATCCTCA AAGCGGTGCG AGACTTGGCC 
CCAGACTCCC TCATCATTGA GTACAGGGGC AAGTTCATGC TCAGACAGCA GTTTGAGGCC 
AACGGATACT TCTTTAAGAG GCCATACCCA TTTGTGTTAT TTTATTCTAA ATTTGACGGA 
CTGGAGATGT GCGTGGACGC TCGTAGCTTC GGCAACGAAG CGCGCTTCAT CCGTCGCTCC 
TGCACCCCTA ATTCTGAAGT GCGGCACGTC ATTGAGGACG GCATGCTGCA TTTATACATC 
TACTCATTGA GGCCGATCAC CAAGGGCACA GAGATCACCA TCGGTTTTGA TTTTGACTAT 
GGCAGCTGTA AATACAAGGT GGACTGTGCC TGCGTGAAGG GGAATCAGGA GTGCCCGGTG 
CTCAAGCACA ACCTCGAACC CACAGAGAAC CTGGTCGGCC GGCGCAGAGG GAGTCGTAAG 
GACAAGGAGA CGGTTCGAGA CGACCTGGGC CAGAACCAGA ACATGGGTCT GGACGGCGAG 
GGGAAGGGCA AGAGCGTGGG CGACGGCAAA CAGAGGAAGC TCTCTCCTCT TCGCCTCTCT 
ATCTCAAACA ACCAGGATCC TGAGTTATAT GAGGATCTAG AAGACAAAAC CTCCGTTAGC 
AATGAAGTAG AGATGGAGTC AGAGGAGCAG ATTGCAGAGA GGAGGAGGAA GATGACACGA 
GAGGAGAGGA AGATGGAGGC CATCCTGCAA GCGTTTGCCC GAATGGAGAA GAGAGAGAAG 
CGAAGGGAGC AGGCGCTGGA AAGAATCGGT AGCATCAAGT CGGAGGTCGG GGGACGCAGT 
GACATCAAGG AGGAGCCTCC AGCCACGCCA GAGACGGCAG ATTCTCCAGC TGTTATGCAG 
CCGCTCCTGG AGGTGAAAGA GGAGCCAGGA TTAAAGCCGG CCAAGGTCAA AGGCTCGAGG 
AACAGGAAAA GTTTCTCAAG GAACCGCACG CACATTGGCC AACAGCGCCG GAGAGCTCGC 
ACCATCAGCA CCTGCTCTGA CCTGCAGCCT GGCTCTCCAA TTGAATCTGT GGAGCCTCTG 
AGCAACGAAG CCCCCGAAGG AGAGACACCC ACCGCACCAG AGCCGGAGGC AATCTCTTCC 
CAAGCACCAG ACACCAGCCC TCCGCACAGC AGCTCACCTG CACCTGACAG AAACCGCACC 
GGGAGCAAGA ACTTCAAAAC TAAAAAGCAC TTTGTGAGTG AGTGGGTGGG AGAGAAGCAG 
CAGGACCGTG GTGCAGTACG AACCCCAGAG CCGGCCCCCG AGAGGCCGCT GAGAATAAGC 
AGTGACCCCG AAGTACTCGC CACACAGCTG AACGCCCTCC CAGGCATGGC CTGCTCTCCA 
CAGGTCTACA GCACGCCCAA ACACTACGTC CGCTTCTCGT CCCCTTTCCT GGCGAACCGC 
AGTCCGACAA CTCCCGGAGT CCCCACTGGG AGACGGCGTT CCAGAGAACT GCCAGAAACG 
CCGCCAACCA CCGGCTCCTG CAAGAAGCGA TGGTTGAAGC AGGCCCTTGA GGAAGAGGGA 
TCCACCAGCC CAGCCAGACG ACCAAGCCTC CTCATGCCCT GTGAGGGTCC TCTTAGCCCC 
TCAATCAACG GAGACTCTGA CAGCCCTCTT CCCTACAATG GCACCTGCTC GCTACCTGAG 
TTGCCCACGC CTTTGAAAAA GCGCCGGTTG AGTCCATTAG ATGCCTGTAT GTCTGAGAGC 
TCCACACCCT ACGGCTCCCC TTGTGCCACG CCCACCAGGG CAGACCAATC GGAGACACCC 
GCAACCCCCA TCTTACTGGC TACCCCACCG CGTCCCAGAA CGGAGGAGCC CAGTACAGAG 
CCCCCGCCCA ACACCCCAAC GCAGACACTT AACGCCCCTC TGGAGAGTGA ATCCTCTGTG 
GAGAGCTCCC CAGAGCTCAG CCGGAAACCC AGTGTACAAG AGGCCGATCG TCCTCCTTCG 
TTGGTCTCCT CTCCCTGCAT CAGGGCTCCC AGTTCAGACG GACTCCCGGC TGAAGCCAAA 
GTGTCGGTTC CTGAGAGTCC ACAGCCTCCG GTCGCCGACT CTGTGGACTG TGGAGAGGAC 
AGGACCGATG GTGGGGTTGT AGAAGGCAGT AGTGAGCCTT CCTCGTCCGC AGAAACATGT 
GCTTCCTCTT TTCCTGGCTG GATAAAAAGC CCAGACAGAG GCCCGACTGG ACCAGCTGGC 
CTGAACTTCT CTCCAGTCAA CTCAAACCTA AGGGACCTCA CCCCCTCACA CACTCTGGAG 
CCCCTGGCGG CTCCCTTCAG GCCCGACGCT GCAGCTGGGG CTGCGGCAGG GTCCACAGCA 
GGGACCGGGT CATTGGTTGG CCCTCAAGCC CCCTTCAGTG AAGGCCAGGG GCAGCTGTTT 
TACCCCTGCT CTGAGGAGGG AGGTTCACTC GGCTTCTCGC GCTCACTGAA TGGGGATGGC 
AGCGGTGAAG GAGGAGGATC AGCGCAGAAC CCCCCACAGA AGAAAAAGGT TTCCCTGCTA 
GAATACAGGA AACGTCAGCG CGAAGCCCGT CGCAGCGGCT CCAAGACCGA GTGCAGCTCT 
CCTGTCTCCA CAGTGCCACC TCTGACCGTG GACGCCTTCC CCGTCGCGCT GGAGACGACA 
AGCGAACCTC CACTACCCCC TGCTCCTACA GCTGTCTGCA ACGCCAACAA CACCATCGTT 
GTTAAAGAGC CCCAGACGAG CGAGGAGGCC GAGACGCCCG GAGAGAAAGG CGAGAAGGAA 
GGAGGAGAAG GACAGTGGAC GTCGTCCACT TCTGTAGAGC AGGCCCGAGA ACGCAGCTAC 
CACAGAGCTC TGCTGCTCAG CAAAGACAAG GACACGGATG GTGAGACAGA AGGTGGAGAT 
ACACCTGCAC TGAGAGATTG CCCGTCTCCA AGTCTTCAGA AGACCCCGAC TCACACCCCC 
TGCTCTCCTG GTCCTCTCGC TCAGTCCACC AATCGGCCAC CGAAGGAGGA AGACGGCGAC 
GGGCAGCCGA GCAAGCCTGC GGGACCCAAG TCGGCTCCTC TGACCCCCAC AAAGCTGCAT 
CCCACCCCGT TACCCTCCTC GCCGGTCCAC TATCCGGGAC CCTCCCTCCT GCACTCGCCC 
AAGCCTCAGC CTCAGGGCTC GCCGTACCGC AGCCAGAGGG CGCTGTTCTC CGCTCAGCCT 
CAAAACCAAC CGCAGCCCCA GGCTCAGACT GGCCCAGCTC CTTTCCCCCA GTATAACACA 
CAGAGTGCTC CACCGCCACC TCCCCCTCCT CCACCAGCAC CACCGGCCTC CACGGCGTAT 
TTTCCCAGCC AGAGCCCCTC GCCTGCTGGA CCTTTCCCCG GGTTCAAACC CGCTGTCACA 
CCCCCTTACC CTCCGTGTTC TCAGCCCCTG ATGCAGACTC TTCCCCACAG CGTGCACTAT 
CAGAGCTCAG CCGCTCCACC GCCGCCGCCT CCTCCGCCCC CGCACCCGAT GCCCGGCCCC 
ACCCTGCTGC ACGTCAACCT GCAGCCCCCT CCCATCCAGC AGCACCAGCT CATGCTGACC 
ACCGCCCCTC CGCCGCCTCC TCCCCCACAG GGCCAGACCT CACAGCAGCA GCAGCAGCAG 
CCTCCCGCTG GCAGCTCCCT GCTGTCGCTC ACCCCTCCTC CGCCGCCTCC TCCACCTCCT 
CCAGCCCCCT CGACCAACGC CCCGATGCAG GCCCACCACT TTCAGAACTT AGGGGGTTTT 
CAGCCGGCGT TGCTACACCC GGGCGGCGCC GCCACAAACC CTTCAGTGCC CCAATCCACG 
TACCCCCCAC CCCTTCAGCA GACCGGACTG CCTCCACCTC CCCCTCCTCC TCCCCAACAG 
ACTCAACAAG GCCAGGCTCA GGCCGCCGCC TCCCAGATGC CTTCTGGGAC TCGTGGAGCT 
CCTGCGTCCT CGACCCCCTT CCACAGCTCG GGGTACCTGA GCACAGGGTG GCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kmt2e ( XM_019278451.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019133996.2 Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X7, mRNA.

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CloneID	OLa211869
Clone ID Related Accession (Same CDS sequence)	XM_019278451.2
Accession Version	XM_019278451.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	inactive histone-lysine N-methyltransferase 2E isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040030.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_019278451.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACAGCAGACA CCTCATACCT GGACATGGCT 
GCGGGCTCAG ACAGACCAGA ATCGGTGGAG GCCAGCCCTG TGGTGGTGGA AAAGTCCAGC 
TACCCACACC AGATCTACAG CAGCAGTTCT CACCATTCCC ACAGTTACAT TGGCCTGCCT 
TACGCTGACC ATAACTATGG GGCGCGGCCC CCACCTACAC CACCAGCCTC CCCTCCCCCC 
TCCATGTTGA TCCGACAAGG AGAGGGGGGG TTGTTTGTTC CAGGGGGCCA GGACGAAGCA 
TCAAGGGGCA CGACGCTCAG CACCTCAGAG GATGGCAGCT ACGGGGCTGA CATCACCCGC 
TGCATCTGTG GCTTCACCCA CGATGACGGC TACATGATCT GCTGTGATAA GTGCAGTGCC 
TGGCAGCATA TAGACTGCAT GGGCATCGAC AGGCAGAACA TTCCTGAGAC CTACCTGTGT 
GAACGCTGCC AACCACGACA CCTGGATCGA GAGAGGGCCA TCCTGCTGCA GACCAGGAAA 
CGGGAATGTT TGTCTGATGG GGACACGAGT GCTACAGAGA GTGGAGATGA GGTGCCGCTA 
GAGCTCTACT CCACCTTCCA ACACACGCCT ACCACCATCA CCCTGACTAC CGCGCGCCTT 
GGCAATAAGC AGGCTGATAA GAAACGCAAA AAGAGCGGTG ATAAAGAGCC TCCAGCGTCT 
TCTGCCCGAG CAAAGAAGGC CTTCCGAGAA GGGTCCAGAA AGTCCTCCAG AGTAAAGGGT 
GCAGCGCCAG AGCAGGAGCC AACAGAGTCC CTGTGGGAGA ACAAGATCAA GACGTGGATG 
GAGCGTTATG AGGAGGCCAG CAGCAATCAG TACAGCGAGG ATGTCCAGGT CCTGTTGCGT 
GTCAAAGAAC AAGGCGATGG CAAGAGCCTG GCGTACAACA CGCACCCTGC CTCTTTCAAA 
CCCCCTGTGG AGAGTCAAGT TCAGAAGAAC AAGAAGATCC TCAAAGCGGT GCGAGACTTG 
GCCCCAGACT CCCTCATCAT TGAGTACAGG GGCAAGTTCA TGCTCAGACA GCAGTTTGAG 
GCCAACGGAT ACTTCTTTAA GAGGCCATAC CCATTTGTGT TATTTTATTC TAAATTTGAC 
GGACTGGAGA TGTGCGTGGA CGCTCGTAGC TTCGGCAACG AAGCGCGCTT CATCCGTCGC 
TCCTGCACCC CTAATTCTGA AGTGCGGCAC GTCATTGAGG ACGGCATGCT GCATTTATAC 
ATCTACTCAT TGAGGCCGAT CACCAAGGGC ACAGAGATCA CCATCGGTTT TGATTTTGAC 
TATGGCAGCT GTAAATACAA GGTGGACTGT GCCTGCGTGA AGGGGAATCA GGAGTGCCCG 
GTGCTCAAGC ACAACCTCGA ACCCACAGAG AACCTGGTCG GCCGGCGCAG AGGGAGTCGT 
AAGGACAAGG AGACGGTTCG AGACGACCTG GGCCAGAACC AGAACATGGG TCTGGACGGC 
GAGGGGAAGG GCAAGAGCGT GGGCGACGGC AAACAGAGGA AGCTCTCTCC TCTTCGCCTC 
TCTATCTCAA ACAACCAGGC CAACCCAGCG GAGCAGTCCC ATCTCCCTGT CGGGGCGGCT 
TCCAGCTGGA AAGAACTGAA ACACAAGGAG ACACGAGAGG AGAGGAAGAT GGAGGCCATC 
CTGCAAGCGT TTGCCCGAAT GGAGAAGAGA GAGAAGCGAA GGGAGCAGGC GCTGGAAAGA 
ATCGGTAGCA TCAAGTCGGA GGTCGGGGGA CGCAGTGACA TCAAGGAGGA GCCTCCAGCC 
ACGCCAGAGA CGGCAGATTC TCCAGCTGTT ATGCAGCCGC TCCTGGAGGT GAAAGAGGAG 
CCAGGATTAA AGCCGGCCAA GGTCAAAGGC TCGAGGAACA GGAAAAGTTT CTCAAGGAAC 
CGCACGCACA TTGGCCAACA GCGCCGGAGA GCTCGCACCA TCAGCACCTG CTCTGACCTG 
CAGCCTGGCT CTCCAATTGA ATCTGTGGAG CCTCTGAGCA ACGAAGCCCC CGAAGGAGAG 
ACACCCACCG CACCAGAGCC GGAGGCAATC TCTTCCCAAG CACCAGACAC CAGCCCTCCG 
CACAGCAGCT CACCTGCACC TGACAGAAAC CGCACCGGGA GCAAGAACTT CAAAACTAAA 
AAGCACTTTG TGAGTGAGTG GGTGGGAGAG AAGCAGCAGG ACCGTGGTGC AGTACGAACC 
CCAGAGCCGG CCCCCGAGAG GCCGCTGAGA ATAAGCAGTG ACCCCGAAGT ACTCGCCACA 
CAGCTGAACG CCCTCCCAGG CATGGCCTGC TCTCCACAGG TCTACAGCAC GCCCAAACAC 
TACGTCCGCT TCTCGTCCCC TTTCCTGGCG AACCGCAGTC CGACAACTCC CGGAGTCCCC 
ACTGGGAGAC GGCGTTCCAG AGAACTGCCA GAAACGCCGC CAACCACCGG CTCCTGCAAG 
AAGCGATGGT TGAAGCAGGC CCTTGAGGAA GAGGGATCCA CCAGCCCAGC CAGACGACCA 
AGCCTCCTCA TGCCCTGTGA GGGTCCTCTT AGCCCCTCAA TCAACGGAGA CTCTGACAGC 
CCTCTTCCCT ACAATGGCAC CTGCTCGCTA CCTGAGTTGC CCACGCCTTT GAAAAAGCGC 
CGGTTGAGTC CATTAGATGC CTGTATGTCT GAGAGCTCCA CACCCTACGG CTCCCCTTGT 
GCCACGCCCA CCAGGGCAGA CCAATCGGAG ACACCCGCAA CCCCCATCTT ACTGGCTACC 
CCACCGCGTC CCAGAACGGA GGAGCCCAGT ACAGAGCCCC CGCCCAACAC CCCAACGCAG 
ACACTTAACG CCCCTCTGGA GAGTGAATCC TCTGTGGAGA GCTCCCCAGA GCTCAGCCGG 
AAACCCAGTG TACAAGAGGC CGATCGTCCT CCTTCGTTGG TCTCCTCTCC CTGCATCAGG 
GCTCCCAGTT CAGACGGACT CCCGGCTGAA GCCAAAGTGT CGGTTCCTGA GAGTCCACAG 
CCTCCGGTCG CCGACTCTGT GGACTGTGGA GAGGACAGGA CCGATGGTGG GGTTGTAGAA 
GGCAGTAGTG AGCCTTCCTC GTCCGCAGAA ACATGTGCTT CCTCTTTTCC TGGCTGGATA 
AAAAGCCCAG ACAGAGGCCC GACTGGACCA GCTGGCCTGA ACTTCTCTCC AGTCAACTCA 
AACCTAAGGG ACCTCACCCC CTCACACACT CTGGAGCCCC TGGCGGCTCC CTTCAGGCCC 
GACGCTGCAG CTGGGGCTGC GGCAGGGTCC ACAGCAGGGA CCGGGTCATT GGTTGGCCCT 
CAAGCCCCCT TCAGTGAAGG CCAGGGGCAG CTGTTTTACC CCTGCTCTGA GGAGGGAGGT 
TCACTCGGCT TCTCGCGCTC ACTGAATGGG GATGGCAGCG GTGAAGGAGG AGGATCAGCG 
CAGAACCCCC CACAGAAGAA AAAGGTTTCC CTGCTAGAAT ACAGGAAACG TCAGCGCGAA 
GCCCGTCGCA GCGGCTCCAA GACCGAGTGC AGCTCTCCTG TCTCCACAGT GCCACCTCTG 
ACCGTGGACG CCTTCCCCGT CGCGCTGGAG ACGACAAGCG AACCTCCACT ACCCCCTGCT 
CCTACAGCTG TCTGCAACGC CAACAACACC ATCGTTGTTA AAGAGCCCCA GACGAGCGAG 
GAGGCCGAGA CGCCCGGAGA GAAAGGCGAG AAGGAAGGAG GAGAAGGACA GTGGACGTCG 
TCCACTTCTG TAGAGCAGGC CCGAGAACGC AGCTACCACA GAGCTCTGCT GCTCAGCAAA 
GACAAGGACA CGGATGGTGA GACAGAAGGT GGAGATACAC CTGCACTGAG AGATTGCCCG 
TCTCCAAGTC TTCAGAAGAC CCCGACTCAC ACCCCCTGCT CTCCTGGTCC TCTCGCTCAG 
TCCACCAATC GGCCACCGAA GGAGGAAGAC GGCGACGGGC AGCCGAGCAA GCCTGCGGGA 
CCCAAGTCGG CTCCTCTGAC CCCCACAAAG CTGCATCCCA CCCCGTTACC CTCCTCGCCG 
GTCCACTATC CGGGACCCTC CCTCCTGCAC TCGCCCAAGC CTCAGCCTCA GGGCTCGCCG 
TACCGCAGCC AGAGGGCGCT GTTCTCCGCT CAGCCTCAAA ACCAACCGCA GCCCCAGGCT 
CAGACTGGCC CAGCTCCTTT CCCCCAGTAT AACACACAGA GTGCTCCACC GCCACCTCCC 
CCTCCTCCAC CAGCACCACC GGCCTCCACG GCGTATTTTC CCAGCCAGAG CCCCTCGCCT 
GCTGGACCTT TCCCCGGGTT CAAACCCGCT GTCACACCCC CTTACCCTCC GTGTTCTCAG 
CCCCTGATGC AGACTCTTCC CCACAGCGTG CACTATCAGA GCTCAGCCGC TCCACCGCCG 
CCGCCTCCTC CGCCCCCGCA CCCGATGCCC GGCCCCACCC TGCTGCACGT CAACCTGCAG 
CCCCCTCCCA TCCAGCAGCA CCAGCTCATG CTGACCACCG CCCCTCCGCC GCCTCCTCCC 
CCACAGGGCC AGACCTCACA GCAGCAGCAG CAGCAGCCTC CCGCTGGCAG CTCCCTGCTG 
TCGCTCACCC CTCCTCCGCC GCCTCCTCCA CCTCCTCCAG CCCCCTCGAC CAACGCCCCG 
ATGCAGGCCC ACCACTTTCA GAACTTAGGG GGTTTTCAGC CGGCGTTGCT ACACCCGGGC 
GGCGCCGCCA CAAACCCTTC AGTGCCCCAA TCCACGTACC CCCCACCCCT TCAGCAGACC 
GGACTGCCTC CACCTCCCCC TCCTCCTCCC CAACAGACTC AACAAGGCCA GGCTCAGGCC 
GCCGCCTCCC AGATGCCTTC TGGGACTCGT GGAGCTCCTG CGTCCTCGAC CCCCTTCCAC 
AGCTCGGGGT ACCTGAGCAC AGGGTGGCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019133996.2
CDS	483..5375
Translation	MSIVIPVGVDTADTSYLDMAAGSDRPESVEASPVVVEKSSYPHQIYSSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPSMLIRQGEGGLFVPGGQDEASRGTTLSTSEDGSYGADITRCICGFTHDDGYMICCDKCSAWQHIDCMGIDRQNIPETYLCERCQPRHLDRERAILLQTRKRECLSDGDTSATESGDEVPLELYSTFQHTPTTITLTTARLGNKQADKKRKKSGDKEPPASSARAKKAFREGSRKSSRVKGAAPEQEPTESLWENKIKTWMERYEEASSNQYSEDVQVLLRVKEQGDGKSLAYNTHPASFKPPVESQVQKNKKILKAVRDLAPDSLIIEYRGKFMLRQQFEANGYFFKRPYPFVLFYSKFDGLEMCVDARSFGNEARFIRRSCTPNSEVRHVIEDGMLHLYIYSLRPITKGTEITIGFDFDYGSCKYKVDCACVKGNQECPVLKHNLEPTENLVGRRRGSRKDKETVRDDLGQNQNMGLDGEGKGKSVGDGKQRKLSPLRLSISNNQANPAEQSHLPVGAASSWKELKHKETREERKMEAILQAFARMEKREKRREQALERIGSIKSEVGGRSDIKEEPPATPETADSPAVMQPLLEVKEEPGLKPAKVKGSRNRKSFSRNRTHIGQQRRRARTISTCSDLQPGSPIESVEPLSNEAPEGETPTAPEPEAISSQAPDTSPPHSSSPAPDRNRTGSKNFKTKKHFVSEWVGEKQQDRGAVRTPEPAPERPLRISSDPEVLATQLNALPGMACSPQVYSTPKHYVRFSSPFLANRSPTTPGVPTGRRRSRELPETPPTTGSCKKRWLKQALEEEGSTSPARRPSLLMPCEGPLSPSINGDSDSPLPYNGTCSLPELPTPLKKRRLSPLDACMSESSTPYGSPCATPTRADQSETPATPILLATPPRPRTEEPSTEPPPNTPTQTLNAPLESESSVESSPELSRKPSVQEADRPPSLVSSPCIRAPSSDGLPAEAKVSVPESPQPPVADSVDCGEDRTDGGVVEGSSEPSSSAETCASSFPGWIKSPDRGPTGPAGLNFSPVNSNLRDLTPSHTLEPLAAPFRPDAAAGAAAGSTAGTGSLVGPQAPFSEGQGQLFYPCSEEGGSLGFSRSLNGDGSGEGGGSAQNPPQKKKVSLLEYRKRQREARRSGSKTECSSPVSTVPPLTVDAFPVALETTSEPPLPPAPTAVCNANNTIVVKEPQTSEEAETPGEKGEKEGGEGQWTSSTSVEQARERSYHRALLLSKDKDTDGETEGGDTPALRDCPSPSLQKTPTHTPCSPGPLAQSTNRPPKEEDGDGQPSKPAGPKSAPLTPTKLHPTPLPSSPVHYPGPSLLHSPKPQPQGSPYRSQRALFSAQPQNQPQPQAQTGPAPFPQYNTQSAPPPPPPPPPAPPASTAYFPSQSPSPAGPFPGFKPAVTPPYPPCSQPLMQTLPHSVHYQSSAAPPPPPPPPPHPMPGPTLLHVNLQPPPIQQHQLMLTTAPPPPPPPQGQTSQQQQQQPPAGSSLLSLTPPPPPPPPPPAPSTNAPMQAHHFQNLGGFQPALLHPGGAATNPSVPQSTYPPPLQQTGLPPPPPPPPQQTQQGQAQAAASQMPSGTRGAPASSTPFHSSGYLSTGWH

Target ORF information:

RefSeq Version	XM_019278451.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019278451.2

ORF Insert Sequence:

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACAGCAGACA CCTCATACCT GGACATGGCT 
GCGGGCTCAG ACAGACCAGA ATCGGTGGAG GCCAGCCCTG TGGTGGTGGA AAAGTCCAGC 
TACCCACACC AGATCTACAG CAGCAGTTCT CACCATTCCC ACAGTTACAT TGGCCTGCCT 
TACGCTGACC ATAACTATGG GGCGCGGCCC CCACCTACAC CACCAGCCTC CCCTCCCCCC 
TCCATGTTGA TCCGACAAGG AGAGGGGGGG TTGTTTGTTC CAGGGGGCCA GGACGAAGCA 
TCAAGGGGCA CGACGCTCAG CACCTCAGAG GATGGCAGCT ACGGGGCTGA CATCACCCGC 
TGCATCTGTG GCTTCACCCA CGATGACGGC TACATGATCT GCTGTGATAA GTGCAGTGCC 
TGGCAGCATA TAGACTGCAT GGGCATCGAC AGGCAGAACA TTCCTGAGAC CTACCTGTGT 
GAACGCTGCC AACCACGACA CCTGGATCGA GAGAGGGCCA TCCTGCTGCA GACCAGGAAA 
CGGGAATGTT TGTCTGATGG GGACACGAGT GCTACAGAGA GTGGAGATGA GGTGCCGCTA 
GAGCTCTACT CCACCTTCCA ACACACGCCT ACCACCATCA CCCTGACTAC CGCGCGCCTT 
GGCAATAAGC AGGCTGATAA GAAACGCAAA AAGAGCGGTG ATAAAGAGCC TCCAGCGTCT 
TCTGCCCGAG CAAAGAAGGC CTTCCGAGAA GGGTCCAGAA AGTCCTCCAG AGTAAAGGGT 
GCAGCGCCAG AGCAGGAGCC AACAGAGTCC CTGTGGGAGA ACAAGATCAA GACGTGGATG 
GAGCGTTATG AGGAGGCCAG CAGCAATCAG TACAGCGAGG ATGTCCAGGT CCTGTTGCGT 
GTCAAAGAAC AAGGCGATGG CAAGAGCCTG GCGTACAACA CGCACCCTGC CTCTTTCAAA 
CCCCCTGTGG AGAGTCAAGT TCAGAAGAAC AAGAAGATCC TCAAAGCGGT GCGAGACTTG 
GCCCCAGACT CCCTCATCAT TGAGTACAGG GGCAAGTTCA TGCTCAGACA GCAGTTTGAG 
GCCAACGGAT ACTTCTTTAA GAGGCCATAC CCATTTGTGT TATTTTATTC TAAATTTGAC 
GGACTGGAGA TGTGCGTGGA CGCTCGTAGC TTCGGCAACG AAGCGCGCTT CATCCGTCGC 
TCCTGCACCC CTAATTCTGA AGTGCGGCAC GTCATTGAGG ACGGCATGCT GCATTTATAC 
ATCTACTCAT TGAGGCCGAT CACCAAGGGC ACAGAGATCA CCATCGGTTT TGATTTTGAC 
TATGGCAGCT GTAAATACAA GGTGGACTGT GCCTGCGTGA AGGGGAATCA GGAGTGCCCG 
GTGCTCAAGC ACAACCTCGA ACCCACAGAG AACCTGGTCG GCCGGCGCAG AGGGAGTCGT 
AAGGACAAGG AGACGGTTCG AGACGACCTG GGCCAGAACC AGAACATGGG TCTGGACGGC 
GAGGGGAAGG GCAAGAGCGT GGGCGACGGC AAACAGAGGA AGCTCTCTCC TCTTCGCCTC 
TCTATCTCAA ACAACCAGGC CAACCCAGCG GAGCAGTCCC ATCTCCCTGT CGGGGCGGCT 
TCCAGCTGGA AAGAACTGAA ACACAAGGAG ACACGAGAGG AGAGGAAGAT GGAGGCCATC 
CTGCAAGCGT TTGCCCGAAT GGAGAAGAGA GAGAAGCGAA GGGAGCAGGC GCTGGAAAGA 
ATCGGTAGCA TCAAGTCGGA GGTCGGGGGA CGCAGTGACA TCAAGGAGGA GCCTCCAGCC 
ACGCCAGAGA CGGCAGATTC TCCAGCTGTT ATGCAGCCGC TCCTGGAGGT GAAAGAGGAG 
CCAGGATTAA AGCCGGCCAA GGTCAAAGGC TCGAGGAACA GGAAAAGTTT CTCAAGGAAC 
CGCACGCACA TTGGCCAACA GCGCCGGAGA GCTCGCACCA TCAGCACCTG CTCTGACCTG 
CAGCCTGGCT CTCCAATTGA ATCTGTGGAG CCTCTGAGCA ACGAAGCCCC CGAAGGAGAG 
ACACCCACCG CACCAGAGCC GGAGGCAATC TCTTCCCAAG CACCAGACAC CAGCCCTCCG 
CACAGCAGCT CACCTGCACC TGACAGAAAC CGCACCGGGA GCAAGAACTT CAAAACTAAA 
AAGCACTTTG TGAGTGAGTG GGTGGGAGAG AAGCAGCAGG ACCGTGGTGC AGTACGAACC 
CCAGAGCCGG CCCCCGAGAG GCCGCTGAGA ATAAGCAGTG ACCCCGAAGT ACTCGCCACA 
CAGCTGAACG CCCTCCCAGG CATGGCCTGC TCTCCACAGG TCTACAGCAC GCCCAAACAC 
TACGTCCGCT TCTCGTCCCC TTTCCTGGCG AACCGCAGTC CGACAACTCC CGGAGTCCCC 
ACTGGGAGAC GGCGTTCCAG AGAACTGCCA GAAACGCCGC CAACCACCGG CTCCTGCAAG 
AAGCGATGGT TGAAGCAGGC CCTTGAGGAA GAGGGATCCA CCAGCCCAGC CAGACGACCA 
AGCCTCCTCA TGCCCTGTGA GGGTCCTCTT AGCCCCTCAA TCAACGGAGA CTCTGACAGC 
CCTCTTCCCT ACAATGGCAC CTGCTCGCTA CCTGAGTTGC CCACGCCTTT GAAAAAGCGC 
CGGTTGAGTC CATTAGATGC CTGTATGTCT GAGAGCTCCA CACCCTACGG CTCCCCTTGT 
GCCACGCCCA CCAGGGCAGA CCAATCGGAG ACACCCGCAA CCCCCATCTT ACTGGCTACC 
CCACCGCGTC CCAGAACGGA GGAGCCCAGT ACAGAGCCCC CGCCCAACAC CCCAACGCAG 
ACACTTAACG CCCCTCTGGA GAGTGAATCC TCTGTGGAGA GCTCCCCAGA GCTCAGCCGG 
AAACCCAGTG TACAAGAGGC CGATCGTCCT CCTTCGTTGG TCTCCTCTCC CTGCATCAGG 
GCTCCCAGTT CAGACGGACT CCCGGCTGAA GCCAAAGTGT CGGTTCCTGA GAGTCCACAG 
CCTCCGGTCG CCGACTCTGT GGACTGTGGA GAGGACAGGA CCGATGGTGG GGTTGTAGAA 
GGCAGTAGTG AGCCTTCCTC GTCCGCAGAA ACATGTGCTT CCTCTTTTCC TGGCTGGATA 
AAAAGCCCAG ACAGAGGCCC GACTGGACCA GCTGGCCTGA ACTTCTCTCC AGTCAACTCA 
AACCTAAGGG ACCTCACCCC CTCACACACT CTGGAGCCCC TGGCGGCTCC CTTCAGGCCC 
GACGCTGCAG CTGGGGCTGC GGCAGGGTCC ACAGCAGGGA CCGGGTCATT GGTTGGCCCT 
CAAGCCCCCT TCAGTGAAGG CCAGGGGCAG CTGTTTTACC CCTGCTCTGA GGAGGGAGGT 
TCACTCGGCT TCTCGCGCTC ACTGAATGGG GATGGCAGCG GTGAAGGAGG AGGATCAGCG 
CAGAACCCCC CACAGAAGAA AAAGGTTTCC CTGCTAGAAT ACAGGAAACG TCAGCGCGAA 
GCCCGTCGCA GCGGCTCCAA GACCGAGTGC AGCTCTCCTG TCTCCACAGT GCCACCTCTG 
ACCGTGGACG CCTTCCCCGT CGCGCTGGAG ACGACAAGCG AACCTCCACT ACCCCCTGCT 
CCTACAGCTG TCTGCAACGC CAACAACACC ATCGTTGTTA AAGAGCCCCA GACGAGCGAG 
GAGGCCGAGA CGCCCGGAGA GAAAGGCGAG AAGGAAGGAG GAGAAGGACA GTGGACGTCG 
TCCACTTCTG TAGAGCAGGC CCGAGAACGC AGCTACCACA GAGCTCTGCT GCTCAGCAAA 
GACAAGGACA CGGATGGTGA GACAGAAGGT GGAGATACAC CTGCACTGAG AGATTGCCCG 
TCTCCAAGTC TTCAGAAGAC CCCGACTCAC ACCCCCTGCT CTCCTGGTCC TCTCGCTCAG 
TCCACCAATC GGCCACCGAA GGAGGAAGAC GGCGACGGGC AGCCGAGCAA GCCTGCGGGA 
CCCAAGTCGG CTCCTCTGAC CCCCACAAAG CTGCATCCCA CCCCGTTACC CTCCTCGCCG 
GTCCACTATC CGGGACCCTC CCTCCTGCAC TCGCCCAAGC CTCAGCCTCA GGGCTCGCCG 
TACCGCAGCC AGAGGGCGCT GTTCTCCGCT CAGCCTCAAA ACCAACCGCA GCCCCAGGCT 
CAGACTGGCC CAGCTCCTTT CCCCCAGTAT AACACACAGA GTGCTCCACC GCCACCTCCC 
CCTCCTCCAC CAGCACCACC GGCCTCCACG GCGTATTTTC CCAGCCAGAG CCCCTCGCCT 
GCTGGACCTT TCCCCGGGTT CAAACCCGCT GTCACACCCC CTTACCCTCC GTGTTCTCAG 
CCCCTGATGC AGACTCTTCC CCACAGCGTG CACTATCAGA GCTCAGCCGC TCCACCGCCG 
CCGCCTCCTC CGCCCCCGCA CCCGATGCCC GGCCCCACCC TGCTGCACGT CAACCTGCAG 
CCCCCTCCCA TCCAGCAGCA CCAGCTCATG CTGACCACCG CCCCTCCGCC GCCTCCTCCC 
CCACAGGGCC AGACCTCACA GCAGCAGCAG CAGCAGCCTC CCGCTGGCAG CTCCCTGCTG 
TCGCTCACCC CTCCTCCGCC GCCTCCTCCA CCTCCTCCAG CCCCCTCGAC CAACGCCCCG 
ATGCAGGCCC ACCACTTTCA GAACTTAGGG GGTTTTCAGC CGGCGTTGCT ACACCCGGGC 
GGCGCCGCCA CAAACCCTTC AGTGCCCCAA TCCACGTACC CCCCACCCCT TCAGCAGACC 
GGACTGCCTC CACCTCCCCC TCCTCCTCCC CAACAGACTC AACAAGGCCA GGCTCAGGCC 
GCCGCCTCCC AGATGCCTTC TGGGACTCGT GGAGCTCCTG CGTCCTCGAC CCCCTTCCAC 
AGCTCGGGGT ACCTGAGCAC AGGGTGGCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kmt2e ( XM_010738913.3 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010737215.3 Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X8, mRNA.

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CloneID	OLa211870
Clone ID Related Accession (Same CDS sequence)	XM_010738913.3
Accession Version	XM_010738913.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	inactive histone-lysine N-methyltransferase 2E isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040030.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_010738913.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACAGCAGACA CCTCATACCT GGACATGGCT 
GCGGGCTCAG ACAGACCAGA ATCGGTGGAG GCCAGCCCTG TGGTGGTGGA AAAGTCCAGC 
TACCCACACC AGATCTACAG CAGCAGTTCT CACCATTCCC ACAGTTACAT TGGCCTGCCT 
TACGCTGACC ATAACTATGG GGCGCGGCCC CCACCTACAC CACCAGCCTC CCCTCCCCCC 
TCCATGTTGA TCCGACAAGG AGAGGGGGGG TTGTTTGTTC CAGGGGGCCA GGACGAAGCA 
TCAAGGGGCA CGACGCTCAG CACCTCAGAG GATGGCAGCT ACGGGGCTGA CATCACCCGC 
TGCATCTGTG GCTTCACCCA CGATGACGGC TACATGATCT GCTGTGATAA GTGCAGTGCC 
TGGCAGCATA TAGACTGCAT GGGCATCGAC AGGCAGAACA TTCCTGAGAC CTACCTGTGT 
GAACGCTGCC AACCACGACA CCTGGATCGA GAGAGGGCCA TCCTGCTGCA GACCAGGAAA 
CGGGAATGTT TGTCTGATGG GGACACGAGT GCTACAGAGA GTGGAGATGA GGTGCCGCTA 
GAGCTCTACT CCACCTTCCA ACACACGCCT ACCACCATCA CCCTGACTAC CGCGCGCCTT 
GGCAATAAGC AGGCTGATAA GAAACGCAAA AAGAGCGGTG ATAAAGAGCC TCCAGCGTCT 
TCTGCCCGAG CAAAGAAGGC CTTCCGAGAA GGGTCCAGAA AGTCCTCCAG AGTAAAGGGT 
GCAGCGCCAG AGCAGGAGCC AACAGAGTCC CTGTGGGAGA ACAAGATCAA GACGTGGATG 
GAGCGTTATG AGGAGGCCAG CAGCAATCAG TACAGCGAGG ATGTCCAGGT CCTGTTGCGT 
GTCAAAGAAC AAGGCGATGG CAAGAGCCTG GCGTACAACA CGCACCCTGC CTCTTTCAAA 
CCCCCTGTGG AGAGTCAAGT TCAGAAGAAC AAGAAGATCC TCAAAGCGGT GCGAGACTTG 
GCCCCAGACT CCCTCATCAT TGAGTACAGG GGCAAGTTCA TGCTCAGACA GCAGTTTGAG 
GCCAACGGAT ACTTCTTTAA GAGGCCATAC CCATTTGTGT TATTTTATTC TAAATTTGAC 
GGACTGGAGA TGTGCGTGGA CGCTCGTAGC TTCGGCAACG AAGCGCGCTT CATCCGTCGC 
TCCTGCACCC CTAATTCTGA AGTGCGGCAC GTCATTGAGG ACGGCATGCT GCATTTATAC 
ATCTACTCAT TGAGGCCGAT CACCAAGGGC ACAGAGATCA CCATCGGTTT TGATTTTGAC 
TATGGCAGCT GTAAATACAA GGTGGACTGT GCCTGCGTGA AGGGGAATCA GGAGTGCCCG 
GTGCTCAAGC ACAACCTCGA ACCCACAGAG AACCTGGTCG GCCGGCGCAG AGGGAGTCGT 
AAGGACAAGG AGACGGTTCG AGACGACCTG GGCCAGAACC AGAACATGGG TCTGGACGGC 
GAGGGGAAGG GCAAGAGCGT GGGCGACGGC AAACAGAGGA AGCTCTCTCC TCTTCGCCTC 
TCTATCTCAA ACAACCAGAC ACGAGAGGAG AGGAAGATGG AGGCCATCCT GCAAGCGTTT 
GCCCGAATGG AGAAGAGAGA GAAGCGAAGG GAGCAGGCGC TGGAAAGAAT CGGTAGCATC 
AAGTCGGAGG TCGGGGGACG CAGTGACATC AAGGAGGAGC CTCCAGCCAC GCCAGAGACG 
GCAGATTCTC CAGCTGTTAT GCAGCCGCTC CTGGAGGTGA AAGAGGAGCC AGGATTAAAG 
CCGGCCAAGG TCAAAGGCTC GAGGAACAGG AAAAGTTTCT CAAGGAACCG CACGCACATT 
GGCCAACAGC GCCGGAGAGC TCGCACCATC AGCACCTGCT CTGACCTGCA GCCTGGCTCT 
CCAATTGAAT CTGTGGAGCC TCTGAGCAAC GAAGCCCCCG AAGGAGAGAC ACCCACCGCA 
CCAGAGCCGG AGGCAATCTC TTCCCAAGCA CCAGACACCA GCCCTCCGCA CAGCAGCTCA 
CCTGCACCTG ACAGAAACCG CACCGGGAGC AAGAACTTCA AAACTAAAAA GCACTTTGTG 
AGTGAGTGGG TGGGAGAGAA GCAGCAGGAC CGTGGTGCAG TACGAACCCC AGAGCCGGCC 
CCCGAGAGGC CGCTGAGAAT AAGCAGTGAC CCCGAAGTAC TCGCCACACA GCTGAACGCC 
CTCCCAGGCA TGGCCTGCTC TCCACAGGTC TACAGCACGC CCAAACACTA CGTCCGCTTC 
TCGTCCCCTT TCCTGGCGAA CCGCAGTCCG ACAACTCCCG GAGTCCCCAC TGGGAGACGG 
CGTTCCAGAG AACTGCCAGA AACGCCGCCA ACCACCGGCT CCTGCAAGAA GCGATGGTTG 
AAGCAGGCCC TTGAGGAAGA GGGATCCACC AGCCCAGCCA GACGACCAAG CCTCCTCATG 
CCCTGTGAGG GTCCTCTTAG CCCCTCAATC AACGGAGACT CTGACAGCCC TCTTCCCTAC 
AATGGCACCT GCTCGCTACC TGAGTTGCCC ACGCCTTTGA AAAAGCGCCG GTTGAGTCCA 
TTAGATGCCT GTATGTCTGA GAGCTCCACA CCCTACGGCT CCCCTTGTGC CACGCCCACC 
AGGGCAGACC AATCGGAGAC ACCCGCAACC CCCATCTTAC TGGCTACCCC ACCGCGTCCC 
AGAACGGAGG AGCCCAGTAC AGAGCCCCCG CCCAACACCC CAACGCAGAC ACTTAACGCC 
CCTCTGGAGA GTGAATCCTC TGTGGAGAGC TCCCCAGAGC TCAGCCGGAA ACCCAGTGTA 
CAAGAGGCCG ATCGTCCTCC TTCGTTGGTC TCCTCTCCCT GCATCAGGGC TCCCAGTTCA 
GACGGACTCC CGGCTGAAGC CAAAGTGTCG GTTCCTGAGA GTCCACAGCC TCCGGTCGCC 
GACTCTGTGG ACTGTGGAGA GGACAGGACC GATGGTGGGG TTGTAGAAGG CAGTAGTGAG 
CCTTCCTCGT CCGCAGAAAC ATGTGCTTCC TCTTTTCCTG GCTGGATAAA AAGCCCAGAC 
AGAGGCCCGA CTGGACCAGC TGGCCTGAAC TTCTCTCCAG TCAACTCAAA CCTAAGGGAC 
CTCACCCCCT CACACACTCT GGAGCCCCTG GCGGCTCCCT TCAGGCCCGA CGCTGCAGCT 
GGGGCTGCGG CAGGGTCCAC AGCAGGGACC GGGTCATTGG TTGGCCCTCA AGCCCCCTTC 
AGTGAAGGCC AGGGGCAGCT GTTTTACCCC TGCTCTGAGG AGGGAGGTTC ACTCGGCTTC 
TCGCGCTCAC TGAATGGGGA TGGCAGCGGT GAAGGAGGAG GATCAGCGCA GAACCCCCCA 
CAGAAGAAAA AGGTTTCCCT GCTAGAATAC AGGAAACGTC AGCGCGAAGC CCGTCGCAGC 
GGCTCCAAGA CCGAGTGCAG CTCTCCTGTC TCCACAGTGC CACCTCTGAC CGTGGACGCC 
TTCCCCGTCG CGCTGGAGAC GACAAGCGAA CCTCCACTAC CCCCTGCTCC TACAGCTGTC 
TGCAACGCCA ACAACACCAT CGTTGTTAAA GAGCCCCAGA CGAGCGAGGA GGCCGAGACG 
CCCGGAGAGA AAGGCGAGAA GGAAGGAGGA GAAGGACAGT GGACGTCGTC CACTTCTGTA 
GAGCAGGCCC GAGAACGCAG CTACCACAGA GCTCTGCTGC TCAGCAAAGA CAAGGACACG 
GATGGTGAGA CAGAAGGTGG AGATACACCT GCACTGAGAG ATTGCCCGTC TCCAAGTCTT 
CAGAAGACCC CGACTCACAC CCCCTGCTCT CCTGGTCCTC TCGCTCAGTC CACCAATCGG 
CCACCGAAGG AGGAAGACGG CGACGGGCAG CCGAGCAAGC CTGCGGGACC CAAGTCGGCT 
CCTCTGACCC CCACAAAGCT GCATCCCACC CCGTTACCCT CCTCGCCGGT CCACTATCCG 
GGACCCTCCC TCCTGCACTC GCCCAAGCCT CAGCCTCAGG GCTCGCCGTA CCGCAGCCAG 
AGGGCGCTGT TCTCCGCTCA GCCTCAAAAC CAACCGCAGC CCCAGGCTCA GACTGGCCCA 
GCTCCTTTCC CCCAGTATAA CACACAGAGT GCTCCACCGC CACCTCCCCC TCCTCCACCA 
GCACCACCGG CCTCCACGGC GTATTTTCCC AGCCAGAGCC CCTCGCCTGC TGGACCTTTC 
CCCGGGTTCA AACCCGCTGT CACACCCCCT TACCCTCCGT GTTCTCAGCC CCTGATGCAG 
ACTCTTCCCC ACAGCGTGCA CTATCAGAGC TCAGCCGCTC CACCGCCGCC GCCTCCTCCG 
CCCCCGCACC CGATGCCCGG CCCCACCCTG CTGCACGTCA ACCTGCAGCC CCCTCCCATC 
CAGCAGCACC AGCTCATGCT GACCACCGCC CCTCCGCCGC CTCCTCCCCC ACAGGGCCAG 
ACCTCACAGC AGCAGCAGCA GCAGCCTCCC GCTGGCAGCT CCCTGCTGTC GCTCACCCCT 
CCTCCGCCGC CTCCTCCACC TCCTCCAGCC CCCTCGACCA ACGCCCCGAT GCAGGCCCAC 
CACTTTCAGA ACTTAGGGGG TTTTCAGCCG GCGTTGCTAC ACCCGGGCGG CGCCGCCACA 
AACCCTTCAG TGCCCCAATC CACGTACCCC CCACCCCTTC AGCAGACCGG ACTGCCTCCA 
CCTCCCCCTC CTCCTCCCCA ACAGACTCAA CAAGGCCAGG CTCAGGCCGC CGCCTCCCAG 
ATGCCTTCTG GGACTCGTGG AGCTCCTGCG TCCTCGACCC CCTTCCACAG CTCGGGGTAC 
CTGAGCACAG GGTGGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010737215.3
CDS	483..5303
Translation	MSIVIPVGVDTADTSYLDMAAGSDRPESVEASPVVVEKSSYPHQIYSSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPSMLIRQGEGGLFVPGGQDEASRGTTLSTSEDGSYGADITRCICGFTHDDGYMICCDKCSAWQHIDCMGIDRQNIPETYLCERCQPRHLDRERAILLQTRKRECLSDGDTSATESGDEVPLELYSTFQHTPTTITLTTARLGNKQADKKRKKSGDKEPPASSARAKKAFREGSRKSSRVKGAAPEQEPTESLWENKIKTWMERYEEASSNQYSEDVQVLLRVKEQGDGKSLAYNTHPASFKPPVESQVQKNKKILKAVRDLAPDSLIIEYRGKFMLRQQFEANGYFFKRPYPFVLFYSKFDGLEMCVDARSFGNEARFIRRSCTPNSEVRHVIEDGMLHLYIYSLRPITKGTEITIGFDFDYGSCKYKVDCACVKGNQECPVLKHNLEPTENLVGRRRGSRKDKETVRDDLGQNQNMGLDGEGKGKSVGDGKQRKLSPLRLSISNNQTREERKMEAILQAFARMEKREKRREQALERIGSIKSEVGGRSDIKEEPPATPETADSPAVMQPLLEVKEEPGLKPAKVKGSRNRKSFSRNRTHIGQQRRRARTISTCSDLQPGSPIESVEPLSNEAPEGETPTAPEPEAISSQAPDTSPPHSSSPAPDRNRTGSKNFKTKKHFVSEWVGEKQQDRGAVRTPEPAPERPLRISSDPEVLATQLNALPGMACSPQVYSTPKHYVRFSSPFLANRSPTTPGVPTGRRRSRELPETPPTTGSCKKRWLKQALEEEGSTSPARRPSLLMPCEGPLSPSINGDSDSPLPYNGTCSLPELPTPLKKRRLSPLDACMSESSTPYGSPCATPTRADQSETPATPILLATPPRPRTEEPSTEPPPNTPTQTLNAPLESESSVESSPELSRKPSVQEADRPPSLVSSPCIRAPSSDGLPAEAKVSVPESPQPPVADSVDCGEDRTDGGVVEGSSEPSSSAETCASSFPGWIKSPDRGPTGPAGLNFSPVNSNLRDLTPSHTLEPLAAPFRPDAAAGAAAGSTAGTGSLVGPQAPFSEGQGQLFYPCSEEGGSLGFSRSLNGDGSGEGGGSAQNPPQKKKVSLLEYRKRQREARRSGSKTECSSPVSTVPPLTVDAFPVALETTSEPPLPPAPTAVCNANNTIVVKEPQTSEEAETPGEKGEKEGGEGQWTSSTSVEQARERSYHRALLLSKDKDTDGETEGGDTPALRDCPSPSLQKTPTHTPCSPGPLAQSTNRPPKEEDGDGQPSKPAGPKSAPLTPTKLHPTPLPSSPVHYPGPSLLHSPKPQPQGSPYRSQRALFSAQPQNQPQPQAQTGPAPFPQYNTQSAPPPPPPPPPAPPASTAYFPSQSPSPAGPFPGFKPAVTPPYPPCSQPLMQTLPHSVHYQSSAAPPPPPPPPPHPMPGPTLLHVNLQPPPIQQHQLMLTTAPPPPPPPQGQTSQQQQQQPPAGSSLLSLTPPPPPPPPPPAPSTNAPMQAHHFQNLGGFQPALLHPGGAATNPSVPQSTYPPPLQQTGLPPPPPPPPQQTQQGQAQAAASQMPSGTRGAPASSTPFHSSGYLSTGWH

Target ORF information:

RefSeq Version	XM_010738913.3
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010738913.3

ORF Insert Sequence:

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACAGCAGACA CCTCATACCT GGACATGGCT 
GCGGGCTCAG ACAGACCAGA ATCGGTGGAG GCCAGCCCTG TGGTGGTGGA AAAGTCCAGC 
TACCCACACC AGATCTACAG CAGCAGTTCT CACCATTCCC ACAGTTACAT TGGCCTGCCT 
TACGCTGACC ATAACTATGG GGCGCGGCCC CCACCTACAC CACCAGCCTC CCCTCCCCCC 
TCCATGTTGA TCCGACAAGG AGAGGGGGGG TTGTTTGTTC CAGGGGGCCA GGACGAAGCA 
TCAAGGGGCA CGACGCTCAG CACCTCAGAG GATGGCAGCT ACGGGGCTGA CATCACCCGC 
TGCATCTGTG GCTTCACCCA CGATGACGGC TACATGATCT GCTGTGATAA GTGCAGTGCC 
TGGCAGCATA TAGACTGCAT GGGCATCGAC AGGCAGAACA TTCCTGAGAC CTACCTGTGT 
GAACGCTGCC AACCACGACA CCTGGATCGA GAGAGGGCCA TCCTGCTGCA GACCAGGAAA 
CGGGAATGTT TGTCTGATGG GGACACGAGT GCTACAGAGA GTGGAGATGA GGTGCCGCTA 
GAGCTCTACT CCACCTTCCA ACACACGCCT ACCACCATCA CCCTGACTAC CGCGCGCCTT 
GGCAATAAGC AGGCTGATAA GAAACGCAAA AAGAGCGGTG ATAAAGAGCC TCCAGCGTCT 
TCTGCCCGAG CAAAGAAGGC CTTCCGAGAA GGGTCCAGAA AGTCCTCCAG AGTAAAGGGT 
GCAGCGCCAG AGCAGGAGCC AACAGAGTCC CTGTGGGAGA ACAAGATCAA GACGTGGATG 
GAGCGTTATG AGGAGGCCAG CAGCAATCAG TACAGCGAGG ATGTCCAGGT CCTGTTGCGT 
GTCAAAGAAC AAGGCGATGG CAAGAGCCTG GCGTACAACA CGCACCCTGC CTCTTTCAAA 
CCCCCTGTGG AGAGTCAAGT TCAGAAGAAC AAGAAGATCC TCAAAGCGGT GCGAGACTTG 
GCCCCAGACT CCCTCATCAT TGAGTACAGG GGCAAGTTCA TGCTCAGACA GCAGTTTGAG 
GCCAACGGAT ACTTCTTTAA GAGGCCATAC CCATTTGTGT TATTTTATTC TAAATTTGAC 
GGACTGGAGA TGTGCGTGGA CGCTCGTAGC TTCGGCAACG AAGCGCGCTT CATCCGTCGC 
TCCTGCACCC CTAATTCTGA AGTGCGGCAC GTCATTGAGG ACGGCATGCT GCATTTATAC 
ATCTACTCAT TGAGGCCGAT CACCAAGGGC ACAGAGATCA CCATCGGTTT TGATTTTGAC 
TATGGCAGCT GTAAATACAA GGTGGACTGT GCCTGCGTGA AGGGGAATCA GGAGTGCCCG 
GTGCTCAAGC ACAACCTCGA ACCCACAGAG AACCTGGTCG GCCGGCGCAG AGGGAGTCGT 
AAGGACAAGG AGACGGTTCG AGACGACCTG GGCCAGAACC AGAACATGGG TCTGGACGGC 
GAGGGGAAGG GCAAGAGCGT GGGCGACGGC AAACAGAGGA AGCTCTCTCC TCTTCGCCTC 
TCTATCTCAA ACAACCAGAC ACGAGAGGAG AGGAAGATGG AGGCCATCCT GCAAGCGTTT 
GCCCGAATGG AGAAGAGAGA GAAGCGAAGG GAGCAGGCGC TGGAAAGAAT CGGTAGCATC 
AAGTCGGAGG TCGGGGGACG CAGTGACATC AAGGAGGAGC CTCCAGCCAC GCCAGAGACG 
GCAGATTCTC CAGCTGTTAT GCAGCCGCTC CTGGAGGTGA AAGAGGAGCC AGGATTAAAG 
CCGGCCAAGG TCAAAGGCTC GAGGAACAGG AAAAGTTTCT CAAGGAACCG CACGCACATT 
GGCCAACAGC GCCGGAGAGC TCGCACCATC AGCACCTGCT CTGACCTGCA GCCTGGCTCT 
CCAATTGAAT CTGTGGAGCC TCTGAGCAAC GAAGCCCCCG AAGGAGAGAC ACCCACCGCA 
CCAGAGCCGG AGGCAATCTC TTCCCAAGCA CCAGACACCA GCCCTCCGCA CAGCAGCTCA 
CCTGCACCTG ACAGAAACCG CACCGGGAGC AAGAACTTCA AAACTAAAAA GCACTTTGTG 
AGTGAGTGGG TGGGAGAGAA GCAGCAGGAC CGTGGTGCAG TACGAACCCC AGAGCCGGCC 
CCCGAGAGGC CGCTGAGAAT AAGCAGTGAC CCCGAAGTAC TCGCCACACA GCTGAACGCC 
CTCCCAGGCA TGGCCTGCTC TCCACAGGTC TACAGCACGC CCAAACACTA CGTCCGCTTC 
TCGTCCCCTT TCCTGGCGAA CCGCAGTCCG ACAACTCCCG GAGTCCCCAC TGGGAGACGG 
CGTTCCAGAG AACTGCCAGA AACGCCGCCA ACCACCGGCT CCTGCAAGAA GCGATGGTTG 
AAGCAGGCCC TTGAGGAAGA GGGATCCACC AGCCCAGCCA GACGACCAAG CCTCCTCATG 
CCCTGTGAGG GTCCTCTTAG CCCCTCAATC AACGGAGACT CTGACAGCCC TCTTCCCTAC 
AATGGCACCT GCTCGCTACC TGAGTTGCCC ACGCCTTTGA AAAAGCGCCG GTTGAGTCCA 
TTAGATGCCT GTATGTCTGA GAGCTCCACA CCCTACGGCT CCCCTTGTGC CACGCCCACC 
AGGGCAGACC AATCGGAGAC ACCCGCAACC CCCATCTTAC TGGCTACCCC ACCGCGTCCC 
AGAACGGAGG AGCCCAGTAC AGAGCCCCCG CCCAACACCC CAACGCAGAC ACTTAACGCC 
CCTCTGGAGA GTGAATCCTC TGTGGAGAGC TCCCCAGAGC TCAGCCGGAA ACCCAGTGTA 
CAAGAGGCCG ATCGTCCTCC TTCGTTGGTC TCCTCTCCCT GCATCAGGGC TCCCAGTTCA 
GACGGACTCC CGGCTGAAGC CAAAGTGTCG GTTCCTGAGA GTCCACAGCC TCCGGTCGCC 
GACTCTGTGG ACTGTGGAGA GGACAGGACC GATGGTGGGG TTGTAGAAGG CAGTAGTGAG 
CCTTCCTCGT CCGCAGAAAC ATGTGCTTCC TCTTTTCCTG GCTGGATAAA AAGCCCAGAC 
AGAGGCCCGA CTGGACCAGC TGGCCTGAAC TTCTCTCCAG TCAACTCAAA CCTAAGGGAC 
CTCACCCCCT CACACACTCT GGAGCCCCTG GCGGCTCCCT TCAGGCCCGA CGCTGCAGCT 
GGGGCTGCGG CAGGGTCCAC AGCAGGGACC GGGTCATTGG TTGGCCCTCA AGCCCCCTTC 
AGTGAAGGCC AGGGGCAGCT GTTTTACCCC TGCTCTGAGG AGGGAGGTTC ACTCGGCTTC 
TCGCGCTCAC TGAATGGGGA TGGCAGCGGT GAAGGAGGAG GATCAGCGCA GAACCCCCCA 
CAGAAGAAAA AGGTTTCCCT GCTAGAATAC AGGAAACGTC AGCGCGAAGC CCGTCGCAGC 
GGCTCCAAGA CCGAGTGCAG CTCTCCTGTC TCCACAGTGC CACCTCTGAC CGTGGACGCC 
TTCCCCGTCG CGCTGGAGAC GACAAGCGAA CCTCCACTAC CCCCTGCTCC TACAGCTGTC 
TGCAACGCCA ACAACACCAT CGTTGTTAAA GAGCCCCAGA CGAGCGAGGA GGCCGAGACG 
CCCGGAGAGA AAGGCGAGAA GGAAGGAGGA GAAGGACAGT GGACGTCGTC CACTTCTGTA 
GAGCAGGCCC GAGAACGCAG CTACCACAGA GCTCTGCTGC TCAGCAAAGA CAAGGACACG 
GATGGTGAGA CAGAAGGTGG AGATACACCT GCACTGAGAG ATTGCCCGTC TCCAAGTCTT 
CAGAAGACCC CGACTCACAC CCCCTGCTCT CCTGGTCCTC TCGCTCAGTC CACCAATCGG 
CCACCGAAGG AGGAAGACGG CGACGGGCAG CCGAGCAAGC CTGCGGGACC CAAGTCGGCT 
CCTCTGACCC CCACAAAGCT GCATCCCACC CCGTTACCCT CCTCGCCGGT CCACTATCCG 
GGACCCTCCC TCCTGCACTC GCCCAAGCCT CAGCCTCAGG GCTCGCCGTA CCGCAGCCAG 
AGGGCGCTGT TCTCCGCTCA GCCTCAAAAC CAACCGCAGC CCCAGGCTCA GACTGGCCCA 
GCTCCTTTCC CCCAGTATAA CACACAGAGT GCTCCACCGC CACCTCCCCC TCCTCCACCA 
GCACCACCGG CCTCCACGGC GTATTTTCCC AGCCAGAGCC CCTCGCCTGC TGGACCTTTC 
CCCGGGTTCA AACCCGCTGT CACACCCCCT TACCCTCCGT GTTCTCAGCC CCTGATGCAG 
ACTCTTCCCC ACAGCGTGCA CTATCAGAGC TCAGCCGCTC CACCGCCGCC GCCTCCTCCG 
CCCCCGCACC CGATGCCCGG CCCCACCCTG CTGCACGTCA ACCTGCAGCC CCCTCCCATC 
CAGCAGCACC AGCTCATGCT GACCACCGCC CCTCCGCCGC CTCCTCCCCC ACAGGGCCAG 
ACCTCACAGC AGCAGCAGCA GCAGCCTCCC GCTGGCAGCT CCCTGCTGTC GCTCACCCCT 
CCTCCGCCGC CTCCTCCACC TCCTCCAGCC CCCTCGACCA ACGCCCCGAT GCAGGCCCAC 
CACTTTCAGA ACTTAGGGGG TTTTCAGCCG GCGTTGCTAC ACCCGGGCGG CGCCGCCACA 
AACCCTTCAG TGCCCCAATC CACGTACCCC CCACCCCTTC AGCAGACCGG ACTGCCTCCA 
CCTCCCCCTC CTCCTCCCCA ACAGACTCAA CAAGGCCAGG CTCAGGCCGC CGCCTCCCAG 
ATGCCTTCTG GGACTCGTGG AGCTCCTGCG TCCTCGACCC CCTTCCACAG CTCGGGGTAC 
CTGAGCACAG GGTGGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kmt2e ( XM_027291951.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_027147752.1 Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OLa211871
Clone ID Related Accession (Same CDS sequence)	XM_027291951.1
Accession Version	XM_027291951.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	inactive histone-lysine N-methyltransferase 2E isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040030.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGATCC GACAAGGAGA GGGGGGGTTG TTTGTTCCAG GGGGCCAGGA CGAAGCATCA 
AGGGGCACGA CGCTCAGCAC CTCAGAGGAT GGCAGCTACG GGGCTGACAT CACCCGCTGC 
ATCTGTGGCT TCACCCACGA TGACGGCTAC ATGATCTGCT GTGATAAGTG CAGTGCCTGG 
CAGCATATAG ACTGCATGGG CATCGACAGG CAGAACATTC CTGAGACCTA CCTGTGTGAA 
CGCTGCCAAC CACGACACCT GGATCGAGAG AGGGCCATCC TGCTGCAGAC CAGGAAACGG 
GAATGTTTGT CTGATGGGGA CACGAGTGCT ACAGAGAGTG GAGATGAGGT GCCGCTAGAG 
CTCTACTCCA CCTTCCAACA CACGCCTACC ACCATCACCC TGACTACCGC GCGCCTTGGC 
AATAAGCAGG CTGATAAGAA ACGCAAAAAG AGCGGTGATA AAGAGCCTCC AGCGTCTTCT 
GCCCGAGCAA AGAAGGCCTT CCGAGAAGGG TCCAGAAAGT CCTCCAGAGT AAAGGGTGCA 
GCGCCAGAGC AGGAGCCAAC AGAGTCCCTG TGGGAGAACA AGATCAAGAC GTGGATGGAG 
CGTTATGAGG AGGCCAGCAG CAATCAGTAC AGCGAGGATG TCCAGGTCCT GTTGCGTGTC 
AAAGAACAAG GCGATGGCAA GAGCCTGGCG TACAACACGC ACCCTGCCTC TTTCAAACCC 
CCTGTGGAGA GTCAAGTTCA GAAGAACAAG AAGATCCTCA AAGCGGTGCG AGACTTGGCC 
CCAGACTCCC TCATCATTGA GTACAGGGGC AAGTTCATGC TCAGACAGCA GTTTGAGGCC 
AACGGATACT TCTTTAAGAG GCCATACCCA TTTGTGTTAT TTTATTCTAA ATTTGACGGA 
CTGGAGATGT GCGTGGACGC TCGTAGCTTC GGCAACGAAG CGCGCTTCAT CCGTCGCTCC 
TGCACCCCTA ATTCTGAAGT GCGGCACGTC ATTGAGGACG GCATGCTGCA TTTATACATC 
TACTCATTGA GGCCGATCAC CAAGGGCACA GAGATCACCA TCGGTTTTGA TTTTGACTAT 
GGCAGCTGTA AATACAAGGT GGACTGTGCC TGCGTGAAGG GGAATCAGGA GTGCCCGGTG 
CTCAAGCACA ACCTCGAACC CACAGAGAAC CTGGTCGGCC GGCGCAGAGG GAGTCGTAAG 
GACAAGGAGA CGGTTCGAGA CGACCTGGGC CAGAACCAGA ACATGGGTCT GGACGGCGAG 
GGGAAGGGCA AGAGCGTGGG CGACGGCAAA CAGAGGAAGC TCTCTCCTCT TCGCCTCTCT 
ATCTCAAACA ACCAGGATCC TGAGTTATAT GAGGATCTAG AAGACAAAAC CTCCGTTAGC 
AATGAAGTAG AGATGGAGTC AGAGGAGCAG ATTGCAGAGA GGAGGAGGAA GATGGCCAAC 
CCAGCGGAGC AGTCCCATCT CCCTGTCGGG GCGGCTTCCA GCTGGAAAGA ACTGAAACAC 
AAGGAGGTAA ACTTTACTCC ACCCATCTAC CAGCCTCCCC ACCACCACCT CTCCCCCAAA 
TCTCCCAGAG AGACACGAGA GGAGAGGAAG ATGGAGGCCA TCCTGCAAGC GTTTGCCCGA 
ATGGAGAAGA GAGAGAAGCG AAGGGAGCAG GCGCTGGAAA GAATCGGTAG CATCAAGTCG 
GAGGTCGGGG GACGCAGTGA CATCAAGGAG GAGCCTCCAG CCACGCCAGA GACGGCAGAT 
TCTCCAGCTG TTATGCAGCC GCTCCTGGAG GTGAAAGAGG AGCCAGGATT AAAGCCGGCC 
AAGGTCAAAG GCTCGAGGAA CAGGAAAAGT TTCTCAAGGA ACCGCACGCA CATTGGCCAA 
CAGCGCCGGA GAGCTCGCAC CATCAGCACC TGCTCTGACC TGCAGCCTGG CTCTCCAATT 
GAATCTGTGG AGCCTCTGAG CAACGAAGCC CCCGAAGGAG AGACACCCAC CGCACCAGAG 
CCGGAGGCAA TCTCTTCCCA AGCACCAGAC ACCAGCCCTC CGCACAGCAG CTCACCTGCA 
CCTGACAGAA ACCGCACCGG GAGCAAGAAC TTCAAAACTA AAAAGCACTT TGTGAGTGAG 
TGGGTGGGAG AGAAGCAGCA GGACCGTGGT GCAGTACGAA CCCCAGAGCC GGCCCCCGAG 
AGGCCGCTGA GAATAAGCAG TGACCCCGAA GTACTCGCCA CACAGCTGAA CGCCCTCCCA 
GGCATGGCCT GCTCTCCACA GGTCTACAGC ACGCCCAAAC ACTACGTCCG CTTCTCGTCC 
CCTTTCCTGG CGAACCGCAG TCCGACAACT CCCGGAGTCC CCACTGGGAG ACGGCGTTCC 
AGAGAACTGC CAGAAACGCC GCCAACCACC GGCTCCTGCA AGAAGCGATG GTTGAAGCAG 
GCCCTTGAGG AAGAGGGATC CACCAGCCCA GCCAGACGAC CAAGCCTCCT CATGCCCTGT 
GAGGGTCCTC TTAGCCCCTC AATCAACGGA GACTCTGACA GCCCTCTTCC CTACAATGGC 
ACCTGCTCGC TACCTGAGTT GCCCACGCCT TTGAAAAAGC GCCGGTTGAG TCCATTAGAT 
GCCTGTATGT CTGAGAGCTC CACACCCTAC GGCTCCCCTT GTGCCACGCC CACCAGGGCA 
GACCAATCGG AGACACCCGC AACCCCCATC TTACTGGCTA CCCCACCGCG TCCCAGAACG 
GAGGAGCCCA GTACAGAGCC CCCGCCCAAC ACCCCAACGC AGACACTTAA CGCCCCTCTG 
GAGAGTGAAT CCTCTGTGGA GAGCTCCCCA GAGCTCAGCC GGAAACCCAG TGTACAAGAG 
GCCGATCGTC CTCCTTCGTT GGTCTCCTCT CCCTGCATCA GGGCTCCCAG TTCAGACGGA 
CTCCCGGCTG AAGCCAAAGT GTCGGTTCCT GAGAGTCCAC AGCCTCCGGT CGCCGACTCT 
GTGGACTGTG GAGAGGACAG GACCGATGGT GGGGTTGTAG AAGGCAGTAG TGAGCCTTCC 
TCGTCCGCAG AAACATGTGC TTCCTCTTTT CCTGGCTGGA TAAAAAGCCC AGACAGAGGC 
CCGACTGGAC CAGCTGGCCT GAACTTCTCT CCAGTCAACT CAAACCTAAG GGACCTCACC 
CCCTCACACA CTCTGGAGCC CCTGGCGGCT CCCTTCAGGC CCGACGCTGC AGCTGGGGCT 
GCGGCAGGGT CCACAGCAGG GACCGGGTCA TTGGTTGGCC CTCAAGCCCC CTTCAGTGAA 
GGCCAGGGGC AGCTGTTTTA CCCCTGCTCT GAGGAGGGAG GTTCACTCGG CTTCTCGCGC 
TCACTGAATG GGGATGGCAG CGGTGAAGGA GGAGGATCAG CGCAGAACCC CCCACAGAAG 
AAAAAGGTTT CCCTGCTAGA ATACAGGAAA CGTCAGCGCG AAGCCCGTCG CAGCGGCTCC 
AAGACCGAGT GCAGCTCTCC TGTCTCCACA GTGCCACCTC TGACCGTGGA CGCCTTCCCC 
GTCGCGCTGG AGACGACAAG CGAACCTCCA CTACCCCCTG CTCCTACAGC TGTCTGCAAC 
GCCAACAACA CCATCGTTGT TAAAGAGCCC CAGACGAGCG AGGAGGCCGA GACGCCCGGA 
GAGAAAGGCG AGAAGGAAGG AGGAGAAGGA CAGTGGACGT CGTCCACTTC TGTAGAGCAG 
GCCCGAGAAC GCAGCTACCA CAGAGCTCTG CTGCTCAGCA AAGACAAGGA CACGGATGGT 
GAGACAGAAG GTGGAGATAC ACCTGCACTG AGAGATTGCC CGTCTCCAAG TCTTCAGAAG 
ACCCCGACTC ACACCCCCTG CTCTCCTGGT CCTCTCGCTC AGTCCACCAA TCGGCCACCG 
AAGGAGGAAG ACGGCGACGG GCAGCCGAGC AAGCCTGCGG GACCCAAGTC GGCTCCTCTG 
ACCCCCACAA AGCTGCATCC CACCCCGTTA CCCTCCTCGC CGGTCCACTA TCCGGGACCC 
TCCCTCCTGC ACTCGCCCAA GCCTCAGCCT CAGGGCTCGC CGTACCGCAG CCAGAGGGCG 
CTGTTCTCCG CTCAGCCTCA AAACCAACCG CAGCCCCAGG CTCAGACTGG CCCAGCTCCT 
TTCCCCCAGT ATAACACACA GAGTGCTCCA CCGCCACCTC CCCCTCCTCC ACCAGCACCA 
CCGGCCTCCA CGGCGTATTT TCCCAGCCAG AGCCCCTCGC CTGCTGGACC TTTCCCCGGG 
TTCAAACCCG CTGTCACACC CCCTTACCCT CCGTGTTCTC AGCCCCTGAT GCAGACTCTT 
CCCCACAGCG TGCACTATCA GAGCTCAGCC GCTCCACCGC CGCCGCCTCC TCCGCCCCCG 
CACCCGATGC CCGGCCCCAC CCTGCTGCAC GTCAACCTGC AGCCCCCTCC CATCCAGCAG 
CACCAGCTCA TGCTGACCAC CGCCCCTCCG CCGCCTCCTC CCCCACAGGG CCAGACCTCA 
CAGCAGCAGC AGCAGCAGCC TCCCGCTGGC AGCTCCCTGC TGTCGCTCAC CCCTCCTCCG 
CCGCCTCCTC CACCTCCTCC AGCCCCCTCG ACCAACGCCC CGATGCAGGC CCACCACTTT 
CAGAACTTAG GGGGTTTTCA GCCGGCGTTG CTACACCCGG GCGGCGCCGC CACAAACCCT 
TCAGTGCCCC AATCCACGTA CCCCCCACCC CTTCAGCAGA CCGGACTGCC TCCACCTCCC 
CCTCCTCCTC CCCAACAGAC TCAACAAGGC CAGGCTCAGG CCGCCGCCTC CCAGATGCCT 
TCTGGGACTC GTGGAGCTCC TGCGTCCTCG ACCCCCTTCC ACAGCTCGGG GTACCTGAGC 
ACAGGGTGGC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027147752.1
CDS	290..5104
Translation	MLIRQGEGGLFVPGGQDEASRGTTLSTSEDGSYGADITRCICGFTHDDGYMICCDKCSAWQHIDCMGIDRQNIPETYLCERCQPRHLDRERAILLQTRKRECLSDGDTSATESGDEVPLELYSTFQHTPTTITLTTARLGNKQADKKRKKSGDKEPPASSARAKKAFREGSRKSSRVKGAAPEQEPTESLWENKIKTWMERYEEASSNQYSEDVQVLLRVKEQGDGKSLAYNTHPASFKPPVESQVQKNKKILKAVRDLAPDSLIIEYRGKFMLRQQFEANGYFFKRPYPFVLFYSKFDGLEMCVDARSFGNEARFIRRSCTPNSEVRHVIEDGMLHLYIYSLRPITKGTEITIGFDFDYGSCKYKVDCACVKGNQECPVLKHNLEPTENLVGRRRGSRKDKETVRDDLGQNQNMGLDGEGKGKSVGDGKQRKLSPLRLSISNNQDPELYEDLEDKTSVSNEVEMESEEQIAERRRKMANPAEQSHLPVGAASSWKELKHKEVNFTPPIYQPPHHHLSPKSPRETREERKMEAILQAFARMEKREKRREQALERIGSIKSEVGGRSDIKEEPPATPETADSPAVMQPLLEVKEEPGLKPAKVKGSRNRKSFSRNRTHIGQQRRRARTISTCSDLQPGSPIESVEPLSNEAPEGETPTAPEPEAISSQAPDTSPPHSSSPAPDRNRTGSKNFKTKKHFVSEWVGEKQQDRGAVRTPEPAPERPLRISSDPEVLATQLNALPGMACSPQVYSTPKHYVRFSSPFLANRSPTTPGVPTGRRRSRELPETPPTTGSCKKRWLKQALEEEGSTSPARRPSLLMPCEGPLSPSINGDSDSPLPYNGTCSLPELPTPLKKRRLSPLDACMSESSTPYGSPCATPTRADQSETPATPILLATPPRPRTEEPSTEPPPNTPTQTLNAPLESESSVESSPELSRKPSVQEADRPPSLVSSPCIRAPSSDGLPAEAKVSVPESPQPPVADSVDCGEDRTDGGVVEGSSEPSSSAETCASSFPGWIKSPDRGPTGPAGLNFSPVNSNLRDLTPSHTLEPLAAPFRPDAAAGAAAGSTAGTGSLVGPQAPFSEGQGQLFYPCSEEGGSLGFSRSLNGDGSGEGGGSAQNPPQKKKVSLLEYRKRQREARRSGSKTECSSPVSTVPPLTVDAFPVALETTSEPPLPPAPTAVCNANNTIVVKEPQTSEEAETPGEKGEKEGGEGQWTSSTSVEQARERSYHRALLLSKDKDTDGETEGGDTPALRDCPSPSLQKTPTHTPCSPGPLAQSTNRPPKEEDGDGQPSKPAGPKSAPLTPTKLHPTPLPSSPVHYPGPSLLHSPKPQPQGSPYRSQRALFSAQPQNQPQPQAQTGPAPFPQYNTQSAPPPPPPPPPAPPASTAYFPSQSPSPAGPFPGFKPAVTPPYPPCSQPLMQTLPHSVHYQSSAAPPPPPPPPPHPMPGPTLLHVNLQPPPIQQHQLMLTTAPPPPPPPQGQTSQQQQQQPPAGSSLLSLTPPPPPPPPPPAPSTNAPMQAHHFQNLGGFQPALLHPGGAATNPSVPQSTYPPPLQQTGLPPPPPPPPQQTQQGQAQAAASQMPSGTRGAPASSTPFHSSGYLSTGWH

Target ORF information:

RefSeq Version	XM_027291951.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027291951.1

ORF Insert Sequence:

ATGTTGATCC GACAAGGAGA GGGGGGGTTG TTTGTTCCAG GGGGCCAGGA CGAAGCATCA 
AGGGGCACGA CGCTCAGCAC CTCAGAGGAT GGCAGCTACG GGGCTGACAT CACCCGCTGC 
ATCTGTGGCT TCACCCACGA TGACGGCTAC ATGATCTGCT GTGATAAGTG CAGTGCCTGG 
CAGCATATAG ACTGCATGGG CATCGACAGG CAGAACATTC CTGAGACCTA CCTGTGTGAA 
CGCTGCCAAC CACGACACCT GGATCGAGAG AGGGCCATCC TGCTGCAGAC CAGGAAACGG 
GAATGTTTGT CTGATGGGGA CACGAGTGCT ACAGAGAGTG GAGATGAGGT GCCGCTAGAG 
CTCTACTCCA CCTTCCAACA CACGCCTACC ACCATCACCC TGACTACCGC GCGCCTTGGC 
AATAAGCAGG CTGATAAGAA ACGCAAAAAG AGCGGTGATA AAGAGCCTCC AGCGTCTTCT 
GCCCGAGCAA AGAAGGCCTT CCGAGAAGGG TCCAGAAAGT CCTCCAGAGT AAAGGGTGCA 
GCGCCAGAGC AGGAGCCAAC AGAGTCCCTG TGGGAGAACA AGATCAAGAC GTGGATGGAG 
CGTTATGAGG AGGCCAGCAG CAATCAGTAC AGCGAGGATG TCCAGGTCCT GTTGCGTGTC 
AAAGAACAAG GCGATGGCAA GAGCCTGGCG TACAACACGC ACCCTGCCTC TTTCAAACCC 
CCTGTGGAGA GTCAAGTTCA GAAGAACAAG AAGATCCTCA AAGCGGTGCG AGACTTGGCC 
CCAGACTCCC TCATCATTGA GTACAGGGGC AAGTTCATGC TCAGACAGCA GTTTGAGGCC 
AACGGATACT TCTTTAAGAG GCCATACCCA TTTGTGTTAT TTTATTCTAA ATTTGACGGA 
CTGGAGATGT GCGTGGACGC TCGTAGCTTC GGCAACGAAG CGCGCTTCAT CCGTCGCTCC 
TGCACCCCTA ATTCTGAAGT GCGGCACGTC ATTGAGGACG GCATGCTGCA TTTATACATC 
TACTCATTGA GGCCGATCAC CAAGGGCACA GAGATCACCA TCGGTTTTGA TTTTGACTAT 
GGCAGCTGTA AATACAAGGT GGACTGTGCC TGCGTGAAGG GGAATCAGGA GTGCCCGGTG 
CTCAAGCACA ACCTCGAACC CACAGAGAAC CTGGTCGGCC GGCGCAGAGG GAGTCGTAAG 
GACAAGGAGA CGGTTCGAGA CGACCTGGGC CAGAACCAGA ACATGGGTCT GGACGGCGAG 
GGGAAGGGCA AGAGCGTGGG CGACGGCAAA CAGAGGAAGC TCTCTCCTCT TCGCCTCTCT 
ATCTCAAACA ACCAGGATCC TGAGTTATAT GAGGATCTAG AAGACAAAAC CTCCGTTAGC 
AATGAAGTAG AGATGGAGTC AGAGGAGCAG ATTGCAGAGA GGAGGAGGAA GATGGCCAAC 
CCAGCGGAGC AGTCCCATCT CCCTGTCGGG GCGGCTTCCA GCTGGAAAGA ACTGAAACAC 
AAGGAGGTAA ACTTTACTCC ACCCATCTAC CAGCCTCCCC ACCACCACCT CTCCCCCAAA 
TCTCCCAGAG AGACACGAGA GGAGAGGAAG ATGGAGGCCA TCCTGCAAGC GTTTGCCCGA 
ATGGAGAAGA GAGAGAAGCG AAGGGAGCAG GCGCTGGAAA GAATCGGTAG CATCAAGTCG 
GAGGTCGGGG GACGCAGTGA CATCAAGGAG GAGCCTCCAG CCACGCCAGA GACGGCAGAT 
TCTCCAGCTG TTATGCAGCC GCTCCTGGAG GTGAAAGAGG AGCCAGGATT AAAGCCGGCC 
AAGGTCAAAG GCTCGAGGAA CAGGAAAAGT TTCTCAAGGA ACCGCACGCA CATTGGCCAA 
CAGCGCCGGA GAGCTCGCAC CATCAGCACC TGCTCTGACC TGCAGCCTGG CTCTCCAATT 
GAATCTGTGG AGCCTCTGAG CAACGAAGCC CCCGAAGGAG AGACACCCAC CGCACCAGAG 
CCGGAGGCAA TCTCTTCCCA AGCACCAGAC ACCAGCCCTC CGCACAGCAG CTCACCTGCA 
CCTGACAGAA ACCGCACCGG GAGCAAGAAC TTCAAAACTA AAAAGCACTT TGTGAGTGAG 
TGGGTGGGAG AGAAGCAGCA GGACCGTGGT GCAGTACGAA CCCCAGAGCC GGCCCCCGAG 
AGGCCGCTGA GAATAAGCAG TGACCCCGAA GTACTCGCCA CACAGCTGAA CGCCCTCCCA 
GGCATGGCCT GCTCTCCACA GGTCTACAGC ACGCCCAAAC ACTACGTCCG CTTCTCGTCC 
CCTTTCCTGG CGAACCGCAG TCCGACAACT CCCGGAGTCC CCACTGGGAG ACGGCGTTCC 
AGAGAACTGC CAGAAACGCC GCCAACCACC GGCTCCTGCA AGAAGCGATG GTTGAAGCAG 
GCCCTTGAGG AAGAGGGATC CACCAGCCCA GCCAGACGAC CAAGCCTCCT CATGCCCTGT 
GAGGGTCCTC TTAGCCCCTC AATCAACGGA GACTCTGACA GCCCTCTTCC CTACAATGGC 
ACCTGCTCGC TACCTGAGTT GCCCACGCCT TTGAAAAAGC GCCGGTTGAG TCCATTAGAT 
GCCTGTATGT CTGAGAGCTC CACACCCTAC GGCTCCCCTT GTGCCACGCC CACCAGGGCA 
GACCAATCGG AGACACCCGC AACCCCCATC TTACTGGCTA CCCCACCGCG TCCCAGAACG 
GAGGAGCCCA GTACAGAGCC CCCGCCCAAC ACCCCAACGC AGACACTTAA CGCCCCTCTG 
GAGAGTGAAT CCTCTGTGGA GAGCTCCCCA GAGCTCAGCC GGAAACCCAG TGTACAAGAG 
GCCGATCGTC CTCCTTCGTT GGTCTCCTCT CCCTGCATCA GGGCTCCCAG TTCAGACGGA 
CTCCCGGCTG AAGCCAAAGT GTCGGTTCCT GAGAGTCCAC AGCCTCCGGT CGCCGACTCT 
GTGGACTGTG GAGAGGACAG GACCGATGGT GGGGTTGTAG AAGGCAGTAG TGAGCCTTCC 
TCGTCCGCAG AAACATGTGC TTCCTCTTTT CCTGGCTGGA TAAAAAGCCC AGACAGAGGC 
CCGACTGGAC CAGCTGGCCT GAACTTCTCT CCAGTCAACT CAAACCTAAG GGACCTCACC 
CCCTCACACA CTCTGGAGCC CCTGGCGGCT CCCTTCAGGC CCGACGCTGC AGCTGGGGCT 
GCGGCAGGGT CCACAGCAGG GACCGGGTCA TTGGTTGGCC CTCAAGCCCC CTTCAGTGAA 
GGCCAGGGGC AGCTGTTTTA CCCCTGCTCT GAGGAGGGAG GTTCACTCGG CTTCTCGCGC 
TCACTGAATG GGGATGGCAG CGGTGAAGGA GGAGGATCAG CGCAGAACCC CCCACAGAAG 
AAAAAGGTTT CCCTGCTAGA ATACAGGAAA CGTCAGCGCG AAGCCCGTCG CAGCGGCTCC 
AAGACCGAGT GCAGCTCTCC TGTCTCCACA GTGCCACCTC TGACCGTGGA CGCCTTCCCC 
GTCGCGCTGG AGACGACAAG CGAACCTCCA CTACCCCCTG CTCCTACAGC TGTCTGCAAC 
GCCAACAACA CCATCGTTGT TAAAGAGCCC CAGACGAGCG AGGAGGCCGA GACGCCCGGA 
GAGAAAGGCG AGAAGGAAGG AGGAGAAGGA CAGTGGACGT CGTCCACTTC TGTAGAGCAG 
GCCCGAGAAC GCAGCTACCA CAGAGCTCTG CTGCTCAGCA AAGACAAGGA CACGGATGGT 
GAGACAGAAG GTGGAGATAC ACCTGCACTG AGAGATTGCC CGTCTCCAAG TCTTCAGAAG 
ACCCCGACTC ACACCCCCTG CTCTCCTGGT CCTCTCGCTC AGTCCACCAA TCGGCCACCG 
AAGGAGGAAG ACGGCGACGG GCAGCCGAGC AAGCCTGCGG GACCCAAGTC GGCTCCTCTG 
ACCCCCACAA AGCTGCATCC CACCCCGTTA CCCTCCTCGC CGGTCCACTA TCCGGGACCC 
TCCCTCCTGC ACTCGCCCAA GCCTCAGCCT CAGGGCTCGC CGTACCGCAG CCAGAGGGCG 
CTGTTCTCCG CTCAGCCTCA AAACCAACCG CAGCCCCAGG CTCAGACTGG CCCAGCTCCT 
TTCCCCCAGT ATAACACACA GAGTGCTCCA CCGCCACCTC CCCCTCCTCC ACCAGCACCA 
CCGGCCTCCA CGGCGTATTT TCCCAGCCAG AGCCCCTCGC CTGCTGGACC TTTCCCCGGG 
TTCAAACCCG CTGTCACACC CCCTTACCCT CCGTGTTCTC AGCCCCTGAT GCAGACTCTT 
CCCCACAGCG TGCACTATCA GAGCTCAGCC GCTCCACCGC CGCCGCCTCC TCCGCCCCCG 
CACCCGATGC CCGGCCCCAC CCTGCTGCAC GTCAACCTGC AGCCCCCTCC CATCCAGCAG 
CACCAGCTCA TGCTGACCAC CGCCCCTCCG CCGCCTCCTC CCCCACAGGG CCAGACCTCA 
CAGCAGCAGC AGCAGCAGCC TCCCGCTGGC AGCTCCCTGC TGTCGCTCAC CCCTCCTCCG 
CCGCCTCCTC CACCTCCTCC AGCCCCCTCG ACCAACGCCC CGATGCAGGC CCACCACTTT 
CAGAACTTAG GGGGTTTTCA GCCGGCGTTG CTACACCCGG GCGGCGCCGC CACAAACCCT 
TCAGTGCCCC AATCCACGTA CCCCCCACCC CTTCAGCAGA CCGGACTGCC TCCACCTCCC 
CCTCCTCCTC CCCAACAGAC TCAACAAGGC CAGGCTCAGG CCGCCGCCTC CCAGATGCCT 
TCTGGGACTC GTGGAGCTCC TGCGTCCTCG ACCCCCTTCC ACAGCTCGGG GTACCTGAGC 
ACAGGGTGGC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kmt2e ( XM_019278447.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019133992.1 Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X1, mRNA.

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CloneID	OLa112873
Clone ID Related Accession (Same CDS sequence)	XM_019278447.1
Accession Version	XM_019278447.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	histone-lysine N-methyltransferase 2E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017609671.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACAGCAGACA CCTCATACCT GGACATGGCT 
GCGGGCTCAG ACAGACCAGA ATCGGTGGAG GCCAGCCCTG TGGTGGTGGA AAAGTCCAGC 
TACCCACACC AGATCTACAG CAGCAGTTCT CACCATTCCC ACAGTTACAT TGGCCTGCCT 
TACGCTGACC ATAACTATGG GGCGCGGCCC CCACCTACAC CACCAGCCTC CCCTCCCCCC 
TCCATGTTGA TCCGACAAGG AGAGGGGGGG TTGTTTGTTC CAGGGGGCCA GGACGAAGCA 
TCAAGGGGCA CGACGCTCAG CACCTCAGAG GATGGCAGCT ACGGGGCTGA CATCACCCGC 
TGCATCTGTG GCTTCACCCA CGATGACGGC TACATGATCT GCTGTGATAA GTGCAGTGCC 
TGGCAGCATA TAGACTGCAT GGGCATCGAC AGGCAGAACA TTCCTGAGAC CTACCTGTGT 
GAACGCTGCC AACCACGACA CCTGGATCGA GAGAGGGCCA TCCTGCTGCA GACCAGGAAA 
CGGGAATGTT TGTCTGATGG GGACACGAGT GCTACAGAGA GTGGAGATGA GGTGCCGCTA 
GAGCTCTACT CCACCTTCCA ACACACGCCT ACCACCATCA CCCTGACTAC CGCGCGCCTT 
GGCAATAAGC AGGCTGATAA GAAACGCAAA AAGAGCGGTG ATAAAGAGCC TCCAGCGTCT 
TCTGCCCGAG CAAAGAAGGC CTTCCGAGAA GGGTCCAGAA AGTCCTCCAG AGTAAAGGGT 
GCAGCGCCAG AGCAGGAGCC AACAGAGTCC CTGTGGGAGA ACAAGATCAA GACGTGGATG 
GAGCGTTATG AGGAGGCCAG CAGCAATCAG TACAGCGAGG ATGTCCAGGT CCTGTTGCGT 
GTCAAAGAAC AAGGCGATGG CAAGAGCCTG GCGTACAACA CGCACCCTGC CTCTTTCAAA 
CCCCCTGTGG AGAGTCAAGT TCAGAAGAAC AAGAAGATCC TCAAAGCGGT GCGAGACTTG 
GCCCCAGACT CCCTCATCAT TGAGTACAGG GGCAAGTTCA TGCTCAGACA GCAGTTTGAG 
GCCAACGGAT ACTTCTTTAA GAGGCCATAC CCATTTGTGT TATTTTATTC TAAATTTGAC 
GGACTGGAGA TGTGCGTGGA CGCTCGTAGC TTCGGCAACG AAGCGCGCTT CATCCGTCGC 
TCCTGCACCC CTAATTCTGA AGTGCGGCAC GTCATTGAGG ACGGCATGCT GCATTTATAC 
ATCTACTCAT TGAGGCCGAT CACCAAGGGC ACAGAGATCA CCATCGGTTT TGATTTTGAC 
TATGGCAGCT GTAAATACAA GGTGGACTGT GCCTGCGTGA AGGGGAATCA GGAGTGCCCG 
GTGCTCAAGC ACAACCTCGA ACCCACAGAG AACCTGGTCG GCCGGCGCAG AGGGAGTCGT 
AAGGACAAGG AGACGGTTCG AGACGACCTG GGCCAGAACC AGAACATGGG TCTGGACGGC 
GAGGGGAAGG GCAAGAGCGT GGGCGACGGC AAACAGAGGA AGCTCTCTCC TCTTCGCCTC 
TCTATCTCAA ACAACCAGGA TCCTGAGTTA TATGAGGATC TAGAAGACAA AACCTCCGTT 
AGCAATGAAG TAGAGATGGA GTCAGAGGAG CAGATTGCAG AGAGGAGGAG GAAGATGGCC 
AACCCAGCGG AGCAGTCCCA TCTCCCTGTC GGGGCGGCTT CCAGCTGGAA AGAACTGAAA 
CACAAGGAGG TAAACTTTAC TCCACCCATC TACCAGCCTC CCCACCACCA CCTCTCCCCC 
AAATCTCCCA GAGAGACACG AGAGGAGAGG AAGATGGAGG CCATCCTGCA AGCGTTTGCC 
CGAATGGAGA AGAGAGAGAA GCGAAGGGAG CAGGCGCTGG AAAGAATCGG TAGCATCAAG 
TCGGAGGTCG GGGGACGCAG TGACATCAAG GAGGAGCCTC CAGCCACGCC AGAGACGGCA 
GATTCTCCAG CTGTTATGCA GCCGCTCCTG GAGGTGAAAG AGGAGCCAGG ATTAAAGCCG 
GCCAAGGTCA AAGGCTCGAG GAACAGGAAA AGTTTCTCAA GGAACCGCAC GCACATTGGC 
CAACAGCGCC GGAGAGCTCG CACCATCAGC ACCTGCTCTG ACCTGCAGCC TGGCTCTCCA 
ATTGAATCTG TGGAGCCTCT GAGCAACGAA GCCCCCGAAG GAGAGACACC CACCGCACCA 
GAGCCGGAGG CAATCTCTTC CCAAGCACCA GACACCAGCC CTCCGCACAG CAGCTCACCT 
GCACCTGACA GAAACCGCAC CGGGAGCAAG AACTTCAAAA CTAAAAAGCA CTTTGTGAGT 
GAGTGGGTGG GAGAGAAGCA GCAGGACCGT GGTGCAGTAC GAACCCCAGA GCCGGCCCCC 
GAGAGGCCGC TGAGAATAAG CAGTGACCCC GAAGTACTCG CCACACAGCT GAACGCCCTC 
CCAGGCATGG CCTGCTCTCC ACAGGTCTAC AGCACGCCCA AACACTACGT CCGCTTCTCG 
TCCCCTTTCC TGGCGAACCG CAGTCCCACA ACTCCCGGAG TCCCCACTGG GAGACGGCGT 
TCCAGAGAAC TGCCAGAAAC GCCGCCAACC ACCGGCTCCT GCAAGAAGCG ATGGTTGAAG 
CAGGCCCTTG AGGAAGAGGG ATCCACCAGC CCAGCCAGAC GACCGAGCCT CCTCATGCCC 
TGTGAGGGTC CTCTTAGCCC CTCCATCAAC GGAGACTCTG ACAGCCCTCT TCCCTACAAT 
GGCACCTGCT CGCTACCTGA GTTGCCCACG CCTTTGAAAA AGCGCCGGTT GAGTCCATTA 
GATGCCTGTA TGTCTGAGAG CTCCACACCC TACGGCTCCC CTTGTGCCAC GCCCACCAGG 
GCAGACCAAT CGGAGACACC CGCAACCCCC ATCTTACTGG CTACCCCACC GCGTCCCAGA 
ACGGAGGAGC CCAGTACAGA GCCCCCGCCC AACACCCCAA CGCAGACACT TAACGCCCCT 
CTGGAGAGTG AATCCTCTGT GGAGAGCTCC CCAGAGCTCA GCCGGAAACC CAGTGTACAA 
GAGGCCGATC GTCCTCCTTC GTTGGTCTCC TCTCCCTGCA TCAGGGCTCC CAGTTCAGAC 
GGACTCCCGG CTGAAGCCAA AGTGTCGGTT CCTGAGAGTC CACAGCCTCC GGTCGCCGAC 
TCTGTGGACT GTGGAGAGGA CAGGACCGAT GGTGGGGTTG TAGAAGGCAG TAGTGAGCCT 
TCCTCGTCCG CAGAAACATG TGCTTCCTCT TTTCCTGGCT GGATAAAAAG CCCAGACAGA 
GGCCCGACTG GACCAGCTGG CCTGAACTTC TCTCCAGTCA ACTCAAACCT AAGGGACCTC 
ACCCCCTCAC ACACTCTGGA GCCCCTGGCG GCTCCCTTCA GGCCCGACGC TGCAGCTGGG 
GCTGCGGCAG GGTCCACAGC AGGGACCGGG TCATTGGTTG GCCCTCAAGC CCCCTTCAGT 
GAAGGCCAGG GGCAGCTGTT TTACCCCTGC TCTGAGGAGG GAGGTTCACT CGGCTTCTCG 
CGCTCACTGA ATGGGGATGG CAGCGGTGAA GGAGGAGGAT CAGCGCAGAA CCCCCCACAG 
AAGAAAAAGG TTTCCCTGCT AGAATACAGG AAACGTCAGC GCGAAGCCCG TCGCAGCGGC 
TCCAAGACCG AGTGCAGCTC TCCCGTCTCC ACCGTGCCAC CTCTGACCGT GGACGCCTTC 
CCCGTCGCGC TGGAGACGAC AAGCGAACCT CCACTACCCC CTGCTCCTAC GGCTGTCTGC 
AACGCCAACA ACACCATCGT TGTTAAAGAG CCCCAGACGA GCGAGGAGGC CGAGACGCCC 
GGAGAGAAAG GCGAGAAGGA AGGAGGAGAA GGACAGTGGA CGTCGTCCAC TTCTGTAGAG 
CAGGCCCGAG AACGCAGCTA CCACAGAGCT CTGCTGCTCA GCAAAGACAA GGACACGGAT 
GGTGAGACAG AAGGTGGAGA TACACCTGCA CTGAGAGATT GCCCGTCTCC AAGTCTTCAG 
AAGACCCCGA CTCACACCCC CTGCTCTCCT GGTCCTCTCG CTCAGCCCAC CAATCGGCCA 
CCGAAGGAGG AAGACGGCGA CGGGCAGCCG AGCAAGCCTG CGGGACCCAA GTCGGCTCCT 
CTGACCCCCA CAAAGCTGCA TCCCACCCCG TTACCCTCCT CGCCGGTCCA CTATCCGGGA 
CCCTCCCTCC TGCACTCGCC CAAGCCTCAG CCTCAGGGCT CGCCGTACCG CAGCCAGAGG 
GCGCTGTTCT CCGCTCAGCC TCAAAACCAA CCGCAGCCCC AGGCTCAGAC TGGCCCAGCT 
CCTTTCCCTC AGTATAACAC ACAGAGTGCT CCACCGCCAC CGCCCCCTCC TCCACCAGCA 
CCACCGGCCT CCACGGCGTA TTTTCCCAGC CAGAGCCCCT CGCCGGCTGG ACCTTTCCCC 
GGGTTCAAAC CCGCCGTCAC GCCCCCTTAC CCTCCGTGTT CTCAGCCCCT GATGCAGACT 
CTTCCCCACA GCGTGCACTA TCAGAGCTCA GCCGCTCCAC CGCCGCCTCC TCCTCCGCCC 
CCGCACCCGA TGCCCGGCCC CACCCTGCTG CACGTCAACC TGCAGCCCCC TCCCATCCAG 
CAGCACCAGC TCATGCTGAC CACCGCCCCT CCGCCGCCTC CTCCCCCACA GGGCCAGACC 
TCACAGCAGC AGCAGCAGCA GCCTCCCGCT GGCAGCTCCC TGCTGTCGCT CACCCCTCCT 
CCGCCGCCTC CTCCACCTCC TCCAGCCCCC TCGACCAACG CCCCGATGCA GGCCCACCAC 
TTTCAGAACT TAGGGGGTTT TCAGCCGGCG TTGCTACACC CGGGCGGCGC CGCCACAAAC 
CCTTCAGTGC CCCAATCCAC GTCCCCCCCA CCCCTTCAGC AGACCGGACT GCCTCCACCT 
CCCCCTCCTC CTCCCCAACA GACTCAACAA GGCCAGGCTC AGGCCGCCGC CTCCCAGATG 
CCTTCTGGGA CTCGTGGAGC TCCTGCGTCC TCGACCCCCT TCCACAGCTC GGGGTACCTG 
AGCACAGGGT GGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019133992.1
CDS	492..5549
Translation	MSIVIPVGVDTADTSYLDMAAGSDRPESVEASPVVVEKSSYPHQIYSSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPSMLIRQGEGGLFVPGGQDEASRGTTLSTSEDGSYGADITRCICGFTHDDGYMICCDKCSAWQHIDCMGIDRQNIPETYLCERCQPRHLDRERAILLQTRKRECLSDGDTSATESGDEVPLELYSTFQHTPTTITLTTARLGNKQADKKRKKSGDKEPPASSARAKKAFREGSRKSSRVKGAAPEQEPTESLWENKIKTWMERYEEASSNQYSEDVQVLLRVKEQGDGKSLAYNTHPASFKPPVESQVQKNKKILKAVRDLAPDSLIIEYRGKFMLRQQFEANGYFFKRPYPFVLFYSKFDGLEMCVDARSFGNEARFIRRSCTPNSEVRHVIEDGMLHLYIYSLRPITKGTEITIGFDFDYGSCKYKVDCACVKGNQECPVLKHNLEPTENLVGRRRGSRKDKETVRDDLGQNQNMGLDGEGKGKSVGDGKQRKLSPLRLSISNNQDPELYEDLEDKTSVSNEVEMESEEQIAERRRKMANPAEQSHLPVGAASSWKELKHKEVNFTPPIYQPPHHHLSPKSPRETREERKMEAILQAFARMEKREKRREQALERIGSIKSEVGGRSDIKEEPPATPETADSPAVMQPLLEVKEEPGLKPAKVKGSRNRKSFSRNRTHIGQQRRRARTISTCSDLQPGSPIESVEPLSNEAPEGETPTAPEPEAISSQAPDTSPPHSSSPAPDRNRTGSKNFKTKKHFVSEWVGEKQQDRGAVRTPEPAPERPLRISSDPEVLATQLNALPGMACSPQVYSTPKHYVRFSSPFLANRSPTTPGVPTGRRRSRELPETPPTTGSCKKRWLKQALEEEGSTSPARRPSLLMPCEGPLSPSINGDSDSPLPYNGTCSLPELPTPLKKRRLSPLDACMSESSTPYGSPCATPTRADQSETPATPILLATPPRPRTEEPSTEPPPNTPTQTLNAPLESESSVESSPELSRKPSVQEADRPPSLVSSPCIRAPSSDGLPAEAKVSVPESPQPPVADSVDCGEDRTDGGVVEGSSEPSSSAETCASSFPGWIKSPDRGPTGPAGLNFSPVNSNLRDLTPSHTLEPLAAPFRPDAAAGAAAGSTAGTGSLVGPQAPFSEGQGQLFYPCSEEGGSLGFSRSLNGDGSGEGGGSAQNPPQKKKVSLLEYRKRQREARRSGSKTECSSPVSTVPPLTVDAFPVALETTSEPPLPPAPTAVCNANNTIVVKEPQTSEEAETPGEKGEKEGGEGQWTSSTSVEQARERSYHRALLLSKDKDTDGETEGGDTPALRDCPSPSLQKTPTHTPCSPGPLAQPTNRPPKEEDGDGQPSKPAGPKSAPLTPTKLHPTPLPSSPVHYPGPSLLHSPKPQPQGSPYRSQRALFSAQPQNQPQPQAQTGPAPFPQYNTQSAPPPPPPPPPAPPASTAYFPSQSPSPAGPFPGFKPAVTPPYPPCSQPLMQTLPHSVHYQSSAAPPPPPPPPPHPMPGPTLLHVNLQPPPIQQHQLMLTTAPPPPPPPQGQTSQQQQQQPPAGSSLLSLTPPPPPPPPPPAPSTNAPMQAHHFQNLGGFQPALLHPGGAATNPSVPQSTSPPPLQQTGLPPPPPPPPQQTQQGQAQAAASQMPSGTRGAPASSTPFHSSGYLSTGWH

Target ORF information:

RefSeq Version	XM_019278447.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019278447.1

ORF Insert Sequence:

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACAGCAGACA CCTCATACCT GGACATGGCT 
GCGGGCTCAG ACAGACCAGA ATCGGTGGAG GCCAGCCCTG TGGTGGTGGA AAAGTCCAGC 
TACCCACACC AGATCTACAG CAGCAGTTCT CACCATTCCC ACAGTTACAT TGGCCTGCCT 
TACGCTGACC ATAACTATGG GGCGCGGCCC CCACCTACAC CACCAGCCTC CCCTCCCCCC 
TCCATGTTGA TCCGACAAGG AGAGGGGGGG TTGTTTGTTC CAGGGGGCCA GGACGAAGCA 
TCAAGGGGCA CGACGCTCAG CACCTCAGAG GATGGCAGCT ACGGGGCTGA CATCACCCGC 
TGCATCTGTG GCTTCACCCA CGATGACGGC TACATGATCT GCTGTGATAA GTGCAGTGCC 
TGGCAGCATA TAGACTGCAT GGGCATCGAC AGGCAGAACA TTCCTGAGAC CTACCTGTGT 
GAACGCTGCC AACCACGACA CCTGGATCGA GAGAGGGCCA TCCTGCTGCA GACCAGGAAA 
CGGGAATGTT TGTCTGATGG GGACACGAGT GCTACAGAGA GTGGAGATGA GGTGCCGCTA 
GAGCTCTACT CCACCTTCCA ACACACGCCT ACCACCATCA CCCTGACTAC CGCGCGCCTT 
GGCAATAAGC AGGCTGATAA GAAACGCAAA AAGAGCGGTG ATAAAGAGCC TCCAGCGTCT 
TCTGCCCGAG CAAAGAAGGC CTTCCGAGAA GGGTCCAGAA AGTCCTCCAG AGTAAAGGGT 
GCAGCGCCAG AGCAGGAGCC AACAGAGTCC CTGTGGGAGA ACAAGATCAA GACGTGGATG 
GAGCGTTATG AGGAGGCCAG CAGCAATCAG TACAGCGAGG ATGTCCAGGT CCTGTTGCGT 
GTCAAAGAAC AAGGCGATGG CAAGAGCCTG GCGTACAACA CGCACCCTGC CTCTTTCAAA 
CCCCCTGTGG AGAGTCAAGT TCAGAAGAAC AAGAAGATCC TCAAAGCGGT GCGAGACTTG 
GCCCCAGACT CCCTCATCAT TGAGTACAGG GGCAAGTTCA TGCTCAGACA GCAGTTTGAG 
GCCAACGGAT ACTTCTTTAA GAGGCCATAC CCATTTGTGT TATTTTATTC TAAATTTGAC 
GGACTGGAGA TGTGCGTGGA CGCTCGTAGC TTCGGCAACG AAGCGCGCTT CATCCGTCGC 
TCCTGCACCC CTAATTCTGA AGTGCGGCAC GTCATTGAGG ACGGCATGCT GCATTTATAC 
ATCTACTCAT TGAGGCCGAT CACCAAGGGC ACAGAGATCA CCATCGGTTT TGATTTTGAC 
TATGGCAGCT GTAAATACAA GGTGGACTGT GCCTGCGTGA AGGGGAATCA GGAGTGCCCG 
GTGCTCAAGC ACAACCTCGA ACCCACAGAG AACCTGGTCG GCCGGCGCAG AGGGAGTCGT 
AAGGACAAGG AGACGGTTCG AGACGACCTG GGCCAGAACC AGAACATGGG TCTGGACGGC 
GAGGGGAAGG GCAAGAGCGT GGGCGACGGC AAACAGAGGA AGCTCTCTCC TCTTCGCCTC 
TCTATCTCAA ACAACCAGGA TCCTGAGTTA TATGAGGATC TAGAAGACAA AACCTCCGTT 
AGCAATGAAG TAGAGATGGA GTCAGAGGAG CAGATTGCAG AGAGGAGGAG GAAGATGGCC 
AACCCAGCGG AGCAGTCCCA TCTCCCTGTC GGGGCGGCTT CCAGCTGGAA AGAACTGAAA 
CACAAGGAGG TAAACTTTAC TCCACCCATC TACCAGCCTC CCCACCACCA CCTCTCCCCC 
AAATCTCCCA GAGAGACACG AGAGGAGAGG AAGATGGAGG CCATCCTGCA AGCGTTTGCC 
CGAATGGAGA AGAGAGAGAA GCGAAGGGAG CAGGCGCTGG AAAGAATCGG TAGCATCAAG 
TCGGAGGTCG GGGGACGCAG TGACATCAAG GAGGAGCCTC CAGCCACGCC AGAGACGGCA 
GATTCTCCAG CTGTTATGCA GCCGCTCCTG GAGGTGAAAG AGGAGCCAGG ATTAAAGCCG 
GCCAAGGTCA AAGGCTCGAG GAACAGGAAA AGTTTCTCAA GGAACCGCAC GCACATTGGC 
CAACAGCGCC GGAGAGCTCG CACCATCAGC ACCTGCTCTG ACCTGCAGCC TGGCTCTCCA 
ATTGAATCTG TGGAGCCTCT GAGCAACGAA GCCCCCGAAG GAGAGACACC CACCGCACCA 
GAGCCGGAGG CAATCTCTTC CCAAGCACCA GACACCAGCC CTCCGCACAG CAGCTCACCT 
GCACCTGACA GAAACCGCAC CGGGAGCAAG AACTTCAAAA CTAAAAAGCA CTTTGTGAGT 
GAGTGGGTGG GAGAGAAGCA GCAGGACCGT GGTGCAGTAC GAACCCCAGA GCCGGCCCCC 
GAGAGGCCGC TGAGAATAAG CAGTGACCCC GAAGTACTCG CCACACAGCT GAACGCCCTC 
CCAGGCATGG CCTGCTCTCC ACAGGTCTAC AGCACGCCCA AACACTACGT CCGCTTCTCG 
TCCCCTTTCC TGGCGAACCG CAGTCCCACA ACTCCCGGAG TCCCCACTGG GAGACGGCGT 
TCCAGAGAAC TGCCAGAAAC GCCGCCAACC ACCGGCTCCT GCAAGAAGCG ATGGTTGAAG 
CAGGCCCTTG AGGAAGAGGG ATCCACCAGC CCAGCCAGAC GACCGAGCCT CCTCATGCCC 
TGTGAGGGTC CTCTTAGCCC CTCCATCAAC GGAGACTCTG ACAGCCCTCT TCCCTACAAT 
GGCACCTGCT CGCTACCTGA GTTGCCCACG CCTTTGAAAA AGCGCCGGTT GAGTCCATTA 
GATGCCTGTA TGTCTGAGAG CTCCACACCC TACGGCTCCC CTTGTGCCAC GCCCACCAGG 
GCAGACCAAT CGGAGACACC CGCAACCCCC ATCTTACTGG CTACCCCACC GCGTCCCAGA 
ACGGAGGAGC CCAGTACAGA GCCCCCGCCC AACACCCCAA CGCAGACACT TAACGCCCCT 
CTGGAGAGTG AATCCTCTGT GGAGAGCTCC CCAGAGCTCA GCCGGAAACC CAGTGTACAA 
GAGGCCGATC GTCCTCCTTC GTTGGTCTCC TCTCCCTGCA TCAGGGCTCC CAGTTCAGAC 
GGACTCCCGG CTGAAGCCAA AGTGTCGGTT CCTGAGAGTC CACAGCCTCC GGTCGCCGAC 
TCTGTGGACT GTGGAGAGGA CAGGACCGAT GGTGGGGTTG TAGAAGGCAG TAGTGAGCCT 
TCCTCGTCCG CAGAAACATG TGCTTCCTCT TTTCCTGGCT GGATAAAAAG CCCAGACAGA 
GGCCCGACTG GACCAGCTGG CCTGAACTTC TCTCCAGTCA ACTCAAACCT AAGGGACCTC 
ACCCCCTCAC ACACTCTGGA GCCCCTGGCG GCTCCCTTCA GGCCCGACGC TGCAGCTGGG 
GCTGCGGCAG GGTCCACAGC AGGGACCGGG TCATTGGTTG GCCCTCAAGC CCCCTTCAGT 
GAAGGCCAGG GGCAGCTGTT TTACCCCTGC TCTGAGGAGG GAGGTTCACT CGGCTTCTCG 
CGCTCACTGA ATGGGGATGG CAGCGGTGAA GGAGGAGGAT CAGCGCAGAA CCCCCCACAG 
AAGAAAAAGG TTTCCCTGCT AGAATACAGG AAACGTCAGC GCGAAGCCCG TCGCAGCGGC 
TCCAAGACCG AGTGCAGCTC TCCCGTCTCC ACCGTGCCAC CTCTGACCGT GGACGCCTTC 
CCCGTCGCGC TGGAGACGAC AAGCGAACCT CCACTACCCC CTGCTCCTAC GGCTGTCTGC 
AACGCCAACA ACACCATCGT TGTTAAAGAG CCCCAGACGA GCGAGGAGGC CGAGACGCCC 
GGAGAGAAAG GCGAGAAGGA AGGAGGAGAA GGACAGTGGA CGTCGTCCAC TTCTGTAGAG 
CAGGCCCGAG AACGCAGCTA CCACAGAGCT CTGCTGCTCA GCAAAGACAA GGACACGGAT 
GGTGAGACAG AAGGTGGAGA TACACCTGCA CTGAGAGATT GCCCGTCTCC AAGTCTTCAG 
AAGACCCCGA CTCACACCCC CTGCTCTCCT GGTCCTCTCG CTCAGCCCAC CAATCGGCCA 
CCGAAGGAGG AAGACGGCGA CGGGCAGCCG AGCAAGCCTG CGGGACCCAA GTCGGCTCCT 
CTGACCCCCA CAAAGCTGCA TCCCACCCCG TTACCCTCCT CGCCGGTCCA CTATCCGGGA 
CCCTCCCTCC TGCACTCGCC CAAGCCTCAG CCTCAGGGCT CGCCGTACCG CAGCCAGAGG 
GCGCTGTTCT CCGCTCAGCC TCAAAACCAA CCGCAGCCCC AGGCTCAGAC TGGCCCAGCT 
CCTTTCCCTC AGTATAACAC ACAGAGTGCT CCACCGCCAC CGCCCCCTCC TCCACCAGCA 
CCACCGGCCT CCACGGCGTA TTTTCCCAGC CAGAGCCCCT CGCCGGCTGG ACCTTTCCCC 
GGGTTCAAAC CCGCCGTCAC GCCCCCTTAC CCTCCGTGTT CTCAGCCCCT GATGCAGACT 
CTTCCCCACA GCGTGCACTA TCAGAGCTCA GCCGCTCCAC CGCCGCCTCC TCCTCCGCCC 
CCGCACCCGA TGCCCGGCCC CACCCTGCTG CACGTCAACC TGCAGCCCCC TCCCATCCAG 
CAGCACCAGC TCATGCTGAC CACCGCCCCT CCGCCGCCTC CTCCCCCACA GGGCCAGACC 
TCACAGCAGC AGCAGCAGCA GCCTCCCGCT GGCAGCTCCC TGCTGTCGCT CACCCCTCCT 
CCGCCGCCTC CTCCACCTCC TCCAGCCCCC TCGACCAACG CCCCGATGCA GGCCCACCAC 
TTTCAGAACT TAGGGGGTTT TCAGCCGGCG TTGCTACACC CGGGCGGCGC CGCCACAAAC 
CCTTCAGTGC CCCAATCCAC GTCCCCCCCA CCCCTTCAGC AGACCGGACT GCCTCCACCT 
CCCCCTCCTC CTCCCCAACA GACTCAACAA GGCCAGGCTC AGGCCGCCGC CTCCCAGATG 
CCTTCTGGGA CTCGTGGAGC TCCTGCGTCC TCGACCCCCT TCCACAGCTC GGGGTACCTG 
AGCACAGGGT GGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kmt2e ( XM_019278448.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019133993.1 Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X2, mRNA.

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CloneID	OLa112874
Clone ID Related Accession (Same CDS sequence)	XM_019278448.1
Accession Version	XM_019278448.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	histone-lysine N-methyltransferase 2E isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017609671.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACAGCAGACA CCTCATACCT GGACATGGCT 
GCGGGCTCAG AACCAGAATC GGTGGAGGCC AGCCCTGTGG TGGTGGAAAA GTCCAGCTAC 
CCACACCAGA TCTACAGCAG CAGTTCTCAC CATTCCCACA GTTACATTGG CCTGCCTTAC 
GCTGACCATA ACTATGGGGC GCGGCCCCCA CCTACACCAC CAGCCTCCCC TCCCCCCTCC 
ATGTTGATCC GACAAGGAGA GGGGGGGTTG TTTGTTCCAG GGGGCCAGGA CGAAGCATCA 
AGGGGCACGA CGCTCAGCAC CTCAGAGGAT GGCAGCTACG GGGCTGACAT CACCCGCTGC 
ATCTGTGGCT TCACCCACGA TGACGGCTAC ATGATCTGCT GTGATAAGTG CAGTGCCTGG 
CAGCATATAG ACTGCATGGG CATCGACAGG CAGAACATTC CTGAGACCTA CCTGTGTGAA 
CGCTGCCAAC CACGACACCT GGATCGAGAG AGGGCCATCC TGCTGCAGAC CAGGAAACGG 
GAATGTTTGT CTGATGGGGA CACGAGTGCT ACAGAGAGTG GAGATGAGGT GCCGCTAGAG 
CTCTACTCCA CCTTCCAACA CACGCCTACC ACCATCACCC TGACTACCGC GCGCCTTGGC 
AATAAGCAGG CTGATAAGAA ACGCAAAAAG AGCGGTGATA AAGAGCCTCC AGCGTCTTCT 
GCCCGAGCAA AGAAGGCCTT CCGAGAAGGG TCCAGAAAGT CCTCCAGAGT AAAGGGTGCA 
GCGCCAGAGC AGGAGCCAAC AGAGTCCCTG TGGGAGAACA AGATCAAGAC GTGGATGGAG 
CGTTATGAGG AGGCCAGCAG CAATCAGTAC AGCGAGGATG TCCAGGTCCT GTTGCGTGTC 
AAAGAACAAG GCGATGGCAA GAGCCTGGCG TACAACACGC ACCCTGCCTC TTTCAAACCC 
CCTGTGGAGA GTCAAGTTCA GAAGAACAAG AAGATCCTCA AAGCGGTGCG AGACTTGGCC 
CCAGACTCCC TCATCATTGA GTACAGGGGC AAGTTCATGC TCAGACAGCA GTTTGAGGCC 
AACGGATACT TCTTTAAGAG GCCATACCCA TTTGTGTTAT TTTATTCTAA ATTTGACGGA 
CTGGAGATGT GCGTGGACGC TCGTAGCTTC GGCAACGAAG CGCGCTTCAT CCGTCGCTCC 
TGCACCCCTA ATTCTGAAGT GCGGCACGTC ATTGAGGACG GCATGCTGCA TTTATACATC 
TACTCATTGA GGCCGATCAC CAAGGGCACA GAGATCACCA TCGGTTTTGA TTTTGACTAT 
GGCAGCTGTA AATACAAGGT GGACTGTGCC TGCGTGAAGG GGAATCAGGA GTGCCCGGTG 
CTCAAGCACA ACCTCGAACC CACAGAGAAC CTGGTCGGCC GGCGCAGAGG GAGTCGTAAG 
GACAAGGAGA CGGTTCGAGA CGACCTGGGC CAGAACCAGA ACATGGGTCT GGACGGCGAG 
GGGAAGGGCA AGAGCGTGGG CGACGGCAAA CAGAGGAAGC TCTCTCCTCT TCGCCTCTCT 
ATCTCAAACA ACCAGGATCC TGAGTTATAT GAGGATCTAG AAGACAAAAC CTCCGTTAGC 
AATGAAGTAG AGATGGAGTC AGAGGAGCAG ATTGCAGAGA GGAGGAGGAA GATGGCCAAC 
CCAGCGGAGC AGTCCCATCT CCCTGTCGGG GCGGCTTCCA GCTGGAAAGA ACTGAAACAC 
AAGGAGGTAA ACTTTACTCC ACCCATCTAC CAGCCTCCCC ACCACCACCT CTCCCCCAAA 
TCTCCCAGAG AGACACGAGA GGAGAGGAAG ATGGAGGCCA TCCTGCAAGC GTTTGCCCGA 
ATGGAGAAGA GAGAGAAGCG AAGGGAGCAG GCGCTGGAAA GAATCGGTAG CATCAAGTCG 
GAGGTCGGGG GACGCAGTGA CATCAAGGAG GAGCCTCCAG CCACGCCAGA GACGGCAGAT 
TCTCCAGCTG TTATGCAGCC GCTCCTGGAG GTGAAAGAGG AGCCAGGATT AAAGCCGGCC 
AAGGTCAAAG GCTCGAGGAA CAGGAAAAGT TTCTCAAGGA ACCGCACGCA CATTGGCCAA 
CAGCGCCGGA GAGCTCGCAC CATCAGCACC TGCTCTGACC TGCAGCCTGG CTCTCCAATT 
GAATCTGTGG AGCCTCTGAG CAACGAAGCC CCCGAAGGAG AGACACCCAC CGCACCAGAG 
CCGGAGGCAA TCTCTTCCCA AGCACCAGAC ACCAGCCCTC CGCACAGCAG CTCACCTGCA 
CCTGACAGAA ACCGCACCGG GAGCAAGAAC TTCAAAACTA AAAAGCACTT TGTGAGTGAG 
TGGGTGGGAG AGAAGCAGCA GGACCGTGGT GCAGTACGAA CCCCAGAGCC GGCCCCCGAG 
AGGCCGCTGA GAATAAGCAG TGACCCCGAA GTACTCGCCA CACAGCTGAA CGCCCTCCCA 
GGCATGGCCT GCTCTCCACA GGTCTACAGC ACGCCCAAAC ACTACGTCCG CTTCTCGTCC 
CCTTTCCTGG CGAACCGCAG TCCCACAACT CCCGGAGTCC CCACTGGGAG ACGGCGTTCC 
AGAGAACTGC CAGAAACGCC GCCAACCACC GGCTCCTGCA AGAAGCGATG GTTGAAGCAG 
GCCCTTGAGG AAGAGGGATC CACCAGCCCA GCCAGACGAC CGAGCCTCCT CATGCCCTGT 
GAGGGTCCTC TTAGCCCCTC CATCAACGGA GACTCTGACA GCCCTCTTCC CTACAATGGC 
ACCTGCTCGC TACCTGAGTT GCCCACGCCT TTGAAAAAGC GCCGGTTGAG TCCATTAGAT 
GCCTGTATGT CTGAGAGCTC CACACCCTAC GGCTCCCCTT GTGCCACGCC CACCAGGGCA 
GACCAATCGG AGACACCCGC AACCCCCATC TTACTGGCTA CCCCACCGCG TCCCAGAACG 
GAGGAGCCCA GTACAGAGCC CCCGCCCAAC ACCCCAACGC AGACACTTAA CGCCCCTCTG 
GAGAGTGAAT CCTCTGTGGA GAGCTCCCCA GAGCTCAGCC GGAAACCCAG TGTACAAGAG 
GCCGATCGTC CTCCTTCGTT GGTCTCCTCT CCCTGCATCA GGGCTCCCAG TTCAGACGGA 
CTCCCGGCTG AAGCCAAAGT GTCGGTTCCT GAGAGTCCAC AGCCTCCGGT CGCCGACTCT 
GTGGACTGTG GAGAGGACAG GACCGATGGT GGGGTTGTAG AAGGCAGTAG TGAGCCTTCC 
TCGTCCGCAG AAACATGTGC TTCCTCTTTT CCTGGCTGGA TAAAAAGCCC AGACAGAGGC 
CCGACTGGAC CAGCTGGCCT GAACTTCTCT CCAGTCAACT CAAACCTAAG GGACCTCACC 
CCCTCACACA CTCTGGAGCC CCTGGCGGCT CCCTTCAGGC CCGACGCTGC AGCTGGGGCT 
GCGGCAGGGT CCACAGCAGG GACCGGGTCA TTGGTTGGCC CTCAAGCCCC CTTCAGTGAA 
GGCCAGGGGC AGCTGTTTTA CCCCTGCTCT GAGGAGGGAG GTTCACTCGG CTTCTCGCGC 
TCACTGAATG GGGATGGCAG CGGTGAAGGA GGAGGATCAG CGCAGAACCC CCCACAGAAG 
AAAAAGGTTT CCCTGCTAGA ATACAGGAAA CGTCAGCGCG AAGCCCGTCG CAGCGGCTCC 
AAGACCGAGT GCAGCTCTCC CGTCTCCACC GTGCCACCTC TGACCGTGGA CGCCTTCCCC 
GTCGCGCTGG AGACGACAAG CGAACCTCCA CTACCCCCTG CTCCTACGGC TGTCTGCAAC 
GCCAACAACA CCATCGTTGT TAAAGAGCCC CAGACGAGCG AGGAGGCCGA GACGCCCGGA 
GAGAAAGGCG AGAAGGAAGG AGGAGAAGGA CAGTGGACGT CGTCCACTTC TGTAGAGCAG 
GCCCGAGAAC GCAGCTACCA CAGAGCTCTG CTGCTCAGCA AAGACAAGGA CACGGATGGT 
GAGACAGAAG GTGGAGATAC ACCTGCACTG AGAGATTGCC CGTCTCCAAG TCTTCAGAAG 
ACCCCGACTC ACACCCCCTG CTCTCCTGGT CCTCTCGCTC AGCCCACCAA TCGGCCACCG 
AAGGAGGAAG ACGGCGACGG GCAGCCGAGC AAGCCTGCGG GACCCAAGTC GGCTCCTCTG 
ACCCCCACAA AGCTGCATCC CACCCCGTTA CCCTCCTCGC CGGTCCACTA TCCGGGACCC 
TCCCTCCTGC ACTCGCCCAA GCCTCAGCCT CAGGGCTCGC CGTACCGCAG CCAGAGGGCG 
CTGTTCTCCG CTCAGCCTCA AAACCAACCG CAGCCCCAGG CTCAGACTGG CCCAGCTCCT 
TTCCCTCAGT ATAACACACA GAGTGCTCCA CCGCCACCGC CCCCTCCTCC ACCAGCACCA 
CCGGCCTCCA CGGCGTATTT TCCCAGCCAG AGCCCCTCGC CGGCTGGACC TTTCCCCGGG 
TTCAAACCCG CCGTCACGCC CCCTTACCCT CCGTGTTCTC AGCCCCTGAT GCAGACTCTT 
CCCCACAGCG TGCACTATCA GAGCTCAGCC GCTCCACCGC CGCCTCCTCC TCCGCCCCCG 
CACCCGATGC CCGGCCCCAC CCTGCTGCAC GTCAACCTGC AGCCCCCTCC CATCCAGCAG 
CACCAGCTCA TGCTGACCAC CGCCCCTCCG CCGCCTCCTC CCCCACAGGG CCAGACCTCA 
CAGCAGCAGC AGCAGCAGCC TCCCGCTGGC AGCTCCCTGC TGTCGCTCAC CCCTCCTCCG 
CCGCCTCCTC CACCTCCTCC AGCCCCCTCG ACCAACGCCC CGATGCAGGC CCACCACTTT 
CAGAACTTAG GGGGTTTTCA GCCGGCGTTG CTACACCCGG GCGGCGCCGC CACAAACCCT 
TCAGTGCCCC AATCCACGTC CCCCCCACCC CTTCAGCAGA CCGGACTGCC TCCACCTCCC 
CCTCCTCCTC CCCAACAGAC TCAACAAGGC CAGGCTCAGG CCGCCGCCTC CCAGATGCCT 
TCTGGGACTC GTGGAGCTCC TGCGTCCTCG ACCCCCTTCC ACAGCTCGGG GTACCTGAGC 
ACAGGGTGGC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019133993.1
CDS	492..5546
Translation	MSIVIPVGVDTADTSYLDMAAGSEPESVEASPVVVEKSSYPHQIYSSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPSMLIRQGEGGLFVPGGQDEASRGTTLSTSEDGSYGADITRCICGFTHDDGYMICCDKCSAWQHIDCMGIDRQNIPETYLCERCQPRHLDRERAILLQTRKRECLSDGDTSATESGDEVPLELYSTFQHTPTTITLTTARLGNKQADKKRKKSGDKEPPASSARAKKAFREGSRKSSRVKGAAPEQEPTESLWENKIKTWMERYEEASSNQYSEDVQVLLRVKEQGDGKSLAYNTHPASFKPPVESQVQKNKKILKAVRDLAPDSLIIEYRGKFMLRQQFEANGYFFKRPYPFVLFYSKFDGLEMCVDARSFGNEARFIRRSCTPNSEVRHVIEDGMLHLYIYSLRPITKGTEITIGFDFDYGSCKYKVDCACVKGNQECPVLKHNLEPTENLVGRRRGSRKDKETVRDDLGQNQNMGLDGEGKGKSVGDGKQRKLSPLRLSISNNQDPELYEDLEDKTSVSNEVEMESEEQIAERRRKMANPAEQSHLPVGAASSWKELKHKEVNFTPPIYQPPHHHLSPKSPRETREERKMEAILQAFARMEKREKRREQALERIGSIKSEVGGRSDIKEEPPATPETADSPAVMQPLLEVKEEPGLKPAKVKGSRNRKSFSRNRTHIGQQRRRARTISTCSDLQPGSPIESVEPLSNEAPEGETPTAPEPEAISSQAPDTSPPHSSSPAPDRNRTGSKNFKTKKHFVSEWVGEKQQDRGAVRTPEPAPERPLRISSDPEVLATQLNALPGMACSPQVYSTPKHYVRFSSPFLANRSPTTPGVPTGRRRSRELPETPPTTGSCKKRWLKQALEEEGSTSPARRPSLLMPCEGPLSPSINGDSDSPLPYNGTCSLPELPTPLKKRRLSPLDACMSESSTPYGSPCATPTRADQSETPATPILLATPPRPRTEEPSTEPPPNTPTQTLNAPLESESSVESSPELSRKPSVQEADRPPSLVSSPCIRAPSSDGLPAEAKVSVPESPQPPVADSVDCGEDRTDGGVVEGSSEPSSSAETCASSFPGWIKSPDRGPTGPAGLNFSPVNSNLRDLTPSHTLEPLAAPFRPDAAAGAAAGSTAGTGSLVGPQAPFSEGQGQLFYPCSEEGGSLGFSRSLNGDGSGEGGGSAQNPPQKKKVSLLEYRKRQREARRSGSKTECSSPVSTVPPLTVDAFPVALETTSEPPLPPAPTAVCNANNTIVVKEPQTSEEAETPGEKGEKEGGEGQWTSSTSVEQARERSYHRALLLSKDKDTDGETEGGDTPALRDCPSPSLQKTPTHTPCSPGPLAQPTNRPPKEEDGDGQPSKPAGPKSAPLTPTKLHPTPLPSSPVHYPGPSLLHSPKPQPQGSPYRSQRALFSAQPQNQPQPQAQTGPAPFPQYNTQSAPPPPPPPPPAPPASTAYFPSQSPSPAGPFPGFKPAVTPPYPPCSQPLMQTLPHSVHYQSSAAPPPPPPPPPHPMPGPTLLHVNLQPPPIQQHQLMLTTAPPPPPPPQGQTSQQQQQQPPAGSSLLSLTPPPPPPPPPPAPSTNAPMQAHHFQNLGGFQPALLHPGGAATNPSVPQSTSPPPLQQTGLPPPPPPPPQQTQQGQAQAAASQMPSGTRGAPASSTPFHSSGYLSTGWH

Target ORF information:

RefSeq Version	XM_019278448.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019278448.1

ORF Insert Sequence:

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACAGCAGACA CCTCATACCT GGACATGGCT 
GCGGGCTCAG AACCAGAATC GGTGGAGGCC AGCCCTGTGG TGGTGGAAAA GTCCAGCTAC 
CCACACCAGA TCTACAGCAG CAGTTCTCAC CATTCCCACA GTTACATTGG CCTGCCTTAC 
GCTGACCATA ACTATGGGGC GCGGCCCCCA CCTACACCAC CAGCCTCCCC TCCCCCCTCC 
ATGTTGATCC GACAAGGAGA GGGGGGGTTG TTTGTTCCAG GGGGCCAGGA CGAAGCATCA 
AGGGGCACGA CGCTCAGCAC CTCAGAGGAT GGCAGCTACG GGGCTGACAT CACCCGCTGC 
ATCTGTGGCT TCACCCACGA TGACGGCTAC ATGATCTGCT GTGATAAGTG CAGTGCCTGG 
CAGCATATAG ACTGCATGGG CATCGACAGG CAGAACATTC CTGAGACCTA CCTGTGTGAA 
CGCTGCCAAC CACGACACCT GGATCGAGAG AGGGCCATCC TGCTGCAGAC CAGGAAACGG 
GAATGTTTGT CTGATGGGGA CACGAGTGCT ACAGAGAGTG GAGATGAGGT GCCGCTAGAG 
CTCTACTCCA CCTTCCAACA CACGCCTACC ACCATCACCC TGACTACCGC GCGCCTTGGC 
AATAAGCAGG CTGATAAGAA ACGCAAAAAG AGCGGTGATA AAGAGCCTCC AGCGTCTTCT 
GCCCGAGCAA AGAAGGCCTT CCGAGAAGGG TCCAGAAAGT CCTCCAGAGT AAAGGGTGCA 
GCGCCAGAGC AGGAGCCAAC AGAGTCCCTG TGGGAGAACA AGATCAAGAC GTGGATGGAG 
CGTTATGAGG AGGCCAGCAG CAATCAGTAC AGCGAGGATG TCCAGGTCCT GTTGCGTGTC 
AAAGAACAAG GCGATGGCAA GAGCCTGGCG TACAACACGC ACCCTGCCTC TTTCAAACCC 
CCTGTGGAGA GTCAAGTTCA GAAGAACAAG AAGATCCTCA AAGCGGTGCG AGACTTGGCC 
CCAGACTCCC TCATCATTGA GTACAGGGGC AAGTTCATGC TCAGACAGCA GTTTGAGGCC 
AACGGATACT TCTTTAAGAG GCCATACCCA TTTGTGTTAT TTTATTCTAA ATTTGACGGA 
CTGGAGATGT GCGTGGACGC TCGTAGCTTC GGCAACGAAG CGCGCTTCAT CCGTCGCTCC 
TGCACCCCTA ATTCTGAAGT GCGGCACGTC ATTGAGGACG GCATGCTGCA TTTATACATC 
TACTCATTGA GGCCGATCAC CAAGGGCACA GAGATCACCA TCGGTTTTGA TTTTGACTAT 
GGCAGCTGTA AATACAAGGT GGACTGTGCC TGCGTGAAGG GGAATCAGGA GTGCCCGGTG 
CTCAAGCACA ACCTCGAACC CACAGAGAAC CTGGTCGGCC GGCGCAGAGG GAGTCGTAAG 
GACAAGGAGA CGGTTCGAGA CGACCTGGGC CAGAACCAGA ACATGGGTCT GGACGGCGAG 
GGGAAGGGCA AGAGCGTGGG CGACGGCAAA CAGAGGAAGC TCTCTCCTCT TCGCCTCTCT 
ATCTCAAACA ACCAGGATCC TGAGTTATAT GAGGATCTAG AAGACAAAAC CTCCGTTAGC 
AATGAAGTAG AGATGGAGTC AGAGGAGCAG ATTGCAGAGA GGAGGAGGAA GATGGCCAAC 
CCAGCGGAGC AGTCCCATCT CCCTGTCGGG GCGGCTTCCA GCTGGAAAGA ACTGAAACAC 
AAGGAGGTAA ACTTTACTCC ACCCATCTAC CAGCCTCCCC ACCACCACCT CTCCCCCAAA 
TCTCCCAGAG AGACACGAGA GGAGAGGAAG ATGGAGGCCA TCCTGCAAGC GTTTGCCCGA 
ATGGAGAAGA GAGAGAAGCG AAGGGAGCAG GCGCTGGAAA GAATCGGTAG CATCAAGTCG 
GAGGTCGGGG GACGCAGTGA CATCAAGGAG GAGCCTCCAG CCACGCCAGA GACGGCAGAT 
TCTCCAGCTG TTATGCAGCC GCTCCTGGAG GTGAAAGAGG AGCCAGGATT AAAGCCGGCC 
AAGGTCAAAG GCTCGAGGAA CAGGAAAAGT TTCTCAAGGA ACCGCACGCA CATTGGCCAA 
CAGCGCCGGA GAGCTCGCAC CATCAGCACC TGCTCTGACC TGCAGCCTGG CTCTCCAATT 
GAATCTGTGG AGCCTCTGAG CAACGAAGCC CCCGAAGGAG AGACACCCAC CGCACCAGAG 
CCGGAGGCAA TCTCTTCCCA AGCACCAGAC ACCAGCCCTC CGCACAGCAG CTCACCTGCA 
CCTGACAGAA ACCGCACCGG GAGCAAGAAC TTCAAAACTA AAAAGCACTT TGTGAGTGAG 
TGGGTGGGAG AGAAGCAGCA GGACCGTGGT GCAGTACGAA CCCCAGAGCC GGCCCCCGAG 
AGGCCGCTGA GAATAAGCAG TGACCCCGAA GTACTCGCCA CACAGCTGAA CGCCCTCCCA 
GGCATGGCCT GCTCTCCACA GGTCTACAGC ACGCCCAAAC ACTACGTCCG CTTCTCGTCC 
CCTTTCCTGG CGAACCGCAG TCCCACAACT CCCGGAGTCC CCACTGGGAG ACGGCGTTCC 
AGAGAACTGC CAGAAACGCC GCCAACCACC GGCTCCTGCA AGAAGCGATG GTTGAAGCAG 
GCCCTTGAGG AAGAGGGATC CACCAGCCCA GCCAGACGAC CGAGCCTCCT CATGCCCTGT 
GAGGGTCCTC TTAGCCCCTC CATCAACGGA GACTCTGACA GCCCTCTTCC CTACAATGGC 
ACCTGCTCGC TACCTGAGTT GCCCACGCCT TTGAAAAAGC GCCGGTTGAG TCCATTAGAT 
GCCTGTATGT CTGAGAGCTC CACACCCTAC GGCTCCCCTT GTGCCACGCC CACCAGGGCA 
GACCAATCGG AGACACCCGC AACCCCCATC TTACTGGCTA CCCCACCGCG TCCCAGAACG 
GAGGAGCCCA GTACAGAGCC CCCGCCCAAC ACCCCAACGC AGACACTTAA CGCCCCTCTG 
GAGAGTGAAT CCTCTGTGGA GAGCTCCCCA GAGCTCAGCC GGAAACCCAG TGTACAAGAG 
GCCGATCGTC CTCCTTCGTT GGTCTCCTCT CCCTGCATCA GGGCTCCCAG TTCAGACGGA 
CTCCCGGCTG AAGCCAAAGT GTCGGTTCCT GAGAGTCCAC AGCCTCCGGT CGCCGACTCT 
GTGGACTGTG GAGAGGACAG GACCGATGGT GGGGTTGTAG AAGGCAGTAG TGAGCCTTCC 
TCGTCCGCAG AAACATGTGC TTCCTCTTTT CCTGGCTGGA TAAAAAGCCC AGACAGAGGC 
CCGACTGGAC CAGCTGGCCT GAACTTCTCT CCAGTCAACT CAAACCTAAG GGACCTCACC 
CCCTCACACA CTCTGGAGCC CCTGGCGGCT CCCTTCAGGC CCGACGCTGC AGCTGGGGCT 
GCGGCAGGGT CCACAGCAGG GACCGGGTCA TTGGTTGGCC CTCAAGCCCC CTTCAGTGAA 
GGCCAGGGGC AGCTGTTTTA CCCCTGCTCT GAGGAGGGAG GTTCACTCGG CTTCTCGCGC 
TCACTGAATG GGGATGGCAG CGGTGAAGGA GGAGGATCAG CGCAGAACCC CCCACAGAAG 
AAAAAGGTTT CCCTGCTAGA ATACAGGAAA CGTCAGCGCG AAGCCCGTCG CAGCGGCTCC 
AAGACCGAGT GCAGCTCTCC CGTCTCCACC GTGCCACCTC TGACCGTGGA CGCCTTCCCC 
GTCGCGCTGG AGACGACAAG CGAACCTCCA CTACCCCCTG CTCCTACGGC TGTCTGCAAC 
GCCAACAACA CCATCGTTGT TAAAGAGCCC CAGACGAGCG AGGAGGCCGA GACGCCCGGA 
GAGAAAGGCG AGAAGGAAGG AGGAGAAGGA CAGTGGACGT CGTCCACTTC TGTAGAGCAG 
GCCCGAGAAC GCAGCTACCA CAGAGCTCTG CTGCTCAGCA AAGACAAGGA CACGGATGGT 
GAGACAGAAG GTGGAGATAC ACCTGCACTG AGAGATTGCC CGTCTCCAAG TCTTCAGAAG 
ACCCCGACTC ACACCCCCTG CTCTCCTGGT CCTCTCGCTC AGCCCACCAA TCGGCCACCG 
AAGGAGGAAG ACGGCGACGG GCAGCCGAGC AAGCCTGCGG GACCCAAGTC GGCTCCTCTG 
ACCCCCACAA AGCTGCATCC CACCCCGTTA CCCTCCTCGC CGGTCCACTA TCCGGGACCC 
TCCCTCCTGC ACTCGCCCAA GCCTCAGCCT CAGGGCTCGC CGTACCGCAG CCAGAGGGCG 
CTGTTCTCCG CTCAGCCTCA AAACCAACCG CAGCCCCAGG CTCAGACTGG CCCAGCTCCT 
TTCCCTCAGT ATAACACACA GAGTGCTCCA CCGCCACCGC CCCCTCCTCC ACCAGCACCA 
CCGGCCTCCA CGGCGTATTT TCCCAGCCAG AGCCCCTCGC CGGCTGGACC TTTCCCCGGG 
TTCAAACCCG CCGTCACGCC CCCTTACCCT CCGTGTTCTC AGCCCCTGAT GCAGACTCTT 
CCCCACAGCG TGCACTATCA GAGCTCAGCC GCTCCACCGC CGCCTCCTCC TCCGCCCCCG 
CACCCGATGC CCGGCCCCAC CCTGCTGCAC GTCAACCTGC AGCCCCCTCC CATCCAGCAG 
CACCAGCTCA TGCTGACCAC CGCCCCTCCG CCGCCTCCTC CCCCACAGGG CCAGACCTCA 
CAGCAGCAGC AGCAGCAGCC TCCCGCTGGC AGCTCCCTGC TGTCGCTCAC CCCTCCTCCG 
CCGCCTCCTC CACCTCCTCC AGCCCCCTCG ACCAACGCCC CGATGCAGGC CCACCACTTT 
CAGAACTTAG GGGGTTTTCA GCCGGCGTTG CTACACCCGG GCGGCGCCGC CACAAACCCT 
TCAGTGCCCC AATCCACGTC CCCCCCACCC CTTCAGCAGA CCGGACTGCC TCCACCTCCC 
CCTCCTCCTC CCCAACAGAC TCAACAAGGC CAGGCTCAGG CCGCCGCCTC CCAGATGCCT 
TCTGGGACTC GTGGAGCTCC TGCGTCCTCG ACCCCCTTCC ACAGCTCGGG GTACCTGAGC 
ACAGGGTGGC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kmt2e ( XM_010738910.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010737212.2 Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X3, mRNA.

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CloneID	OLa112875
Clone ID Related Accession (Same CDS sequence)	XM_010738910.2
Accession Version	XM_010738910.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	histone-lysine N-methyltransferase 2E isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017609671.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 29, 2016 this sequence version replaced XM_010738910.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACAGCAGACA CCTCATACCT GGACATGGCT 
GCGGGCTCAG ACAGACCAGA ATCGGTGGAG GCCAGCCCTG TGGTGGTGGA AAAGTCCAGC 
TACCCACACC AGATCTACAG CAGCAGTTCT CACCATTCCC ACAGTTACAT TGGCCTGCCT 
TACGCTGACC ATAACTATGG GGCGCGGCCC CCACCTACAC CACCAGCCTC CCCTCCCCCC 
TCCATGTTGA TCCGACAAGG AGAGGGGGGG TTGTTTGTTC CAGGGGGCCA GGACGAAGCA 
TCAAGGGGCA CGACGCTCAG CACCTCAGAG GATGGCAGCT ACGGGGCTGA CATCACCCGC 
TGCATCTGTG GCTTCACCCA CGATGACGGC TACATGATCT GCTGTGATAA GTGCAGTGCC 
TGGCAGCATA TAGACTGCAT GGGCATCGAC AGGCAGAACA TTCCTGAGAC CTACCTGTGT 
GAACGCTGCC AACCACGACA CCTGGATCGA GAGAGGGCCA TCCTGCTGCA GACCAGGAAA 
CGGGAATGTT TGTCTGATGG GGACACGAGT GCTACAGAGA GTGGAGATGA GGTGCCGCTA 
GAGCTCTACT CCACCTTCCA ACACACGCCT ACCACCATCA CCCTGACTAC CGCGCGCCTT 
GGCAATAAGC AGGCTGATAA GAAACGCAAA AAGAGCGGTG ATAAAGAGCC TCCAGCGTCT 
TCTGCCCGAG CAAAGAAGGC CTTCCGAGAA GGGTCCAGAA AGTCCTCCAG AGTAAAGGGT 
GCAGCGCCAG AGCAGGAGCC AACAGAGTCC CTGTGGGAGA ACAAGATCAA GACGTGGATG 
GAGCGTTATG AGGAGGCCAG CAGCAATCAG TACAGCGAGG ATGTCCAGGT CCTGTTGCGT 
GTCAAAGAAC AAGGCGATGG CAAGAGCCTG GCGTACAACA CGCACCCTGC CTCTTTCAAA 
CCCCCTGTGG AGAGTCAAGT TCAGAAGAAC AAGAAGATCC TCAAAGCGGT GCGAGACTTG 
GCCCCAGACT CCCTCATCAT TGAGTACAGG GGCAAGTTCA TGCTCAGACA GCAGTTTGAG 
GCCAACGGAT ACTTCTTTAA GAGGCCATAC CCATTTGTGT TATTTTATTC TAAATTTGAC 
GGACTGGAGA TGTGCGTGGA CGCTCGTAGC TTCGGCAACG AAGCGCGCTT CATCCGTCGC 
TCCTGCACCC CTAATTCTGA AGTGCGGCAC GTCATTGAGG ACGGCATGCT GCATTTATAC 
ATCTACTCAT TGAGGCCGAT CACCAAGGGC ACAGAGATCA CCATCGGTTT TGATTTTGAC 
TATGGCAGCT GTAAATACAA GGTGGACTGT GCCTGCGTGA AGGGGAATCA GGAGTGCCCG 
GTGCTCAAGC ACAACCTCGA ACCCACAGAG AACCTGGTCG GCCGGCGCAG AGGGAGTCGT 
AAGGACAAGG AGACGGTTCG AGACGACCTG GGCCAGAACC AGAACATGGG TCTGGACGGC 
GAGGGGAAGG GCAAGAGCGT GGGCGACGGC AAACAGAGGA AGCTCTCTCC TCTTCGCCTC 
TCTATCTCAA ACAACCAGGA TCCTGAGTTA TATGAGGATC TAGAAGACAA AACCTCCGTT 
AGCAATGAAG TAGAGATGGA GTCAGAGGAG CAGATTGCAG AGAGGAGGAG GAAGATGGCC 
AACCCAGCGG AGCAGTCCCA TCTCCCTGTC GGGGCGGCTT CCAGCTGGAA AGAACTGAAA 
CACAAGGAGA CACGAGAGGA GAGGAAGATG GAGGCCATCC TGCAAGCGTT TGCCCGAATG 
GAGAAGAGAG AGAAGCGAAG GGAGCAGGCG CTGGAAAGAA TCGGTAGCAT CAAGTCGGAG 
GTCGGGGGAC GCAGTGACAT CAAGGAGGAG CCTCCAGCCA CGCCAGAGAC GGCAGATTCT 
CCAGCTGTTA TGCAGCCGCT CCTGGAGGTG AAAGAGGAGC CAGGATTAAA GCCGGCCAAG 
GTCAAAGGCT CGAGGAACAG GAAAAGTTTC TCAAGGAACC GCACGCACAT TGGCCAACAG 
CGCCGGAGAG CTCGCACCAT CAGCACCTGC TCTGACCTGC AGCCTGGCTC TCCAATTGAA 
TCTGTGGAGC CTCTGAGCAA CGAAGCCCCC GAAGGAGAGA CACCCACCGC ACCAGAGCCG 
GAGGCAATCT CTTCCCAAGC ACCAGACACC AGCCCTCCGC ACAGCAGCTC ACCTGCACCT 
GACAGAAACC GCACCGGGAG CAAGAACTTC AAAACTAAAA AGCACTTTGT GAGTGAGTGG 
GTGGGAGAGA AGCAGCAGGA CCGTGGTGCA GTACGAACCC CAGAGCCGGC CCCCGAGAGG 
CCGCTGAGAA TAAGCAGTGA CCCCGAAGTA CTCGCCACAC AGCTGAACGC CCTCCCAGGC 
ATGGCCTGCT CTCCACAGGT CTACAGCACG CCCAAACACT ACGTCCGCTT CTCGTCCCCT 
TTCCTGGCGA ACCGCAGTCC CACAACTCCC GGAGTCCCCA CTGGGAGACG GCGTTCCAGA 
GAACTGCCAG AAACGCCGCC AACCACCGGC TCCTGCAAGA AGCGATGGTT GAAGCAGGCC 
CTTGAGGAAG AGGGATCCAC CAGCCCAGCC AGACGACCGA GCCTCCTCAT GCCCTGTGAG 
GGTCCTCTTA GCCCCTCCAT CAACGGAGAC TCTGACAGCC CTCTTCCCTA CAATGGCACC 
TGCTCGCTAC CTGAGTTGCC CACGCCTTTG AAAAAGCGCC GGTTGAGTCC ATTAGATGCC 
TGTATGTCTG AGAGCTCCAC ACCCTACGGC TCCCCTTGTG CCACGCCCAC CAGGGCAGAC 
CAATCGGAGA CACCCGCAAC CCCCATCTTA CTGGCTACCC CACCGCGTCC CAGAACGGAG 
GAGCCCAGTA CAGAGCCCCC GCCCAACACC CCAACGCAGA CACTTAACGC CCCTCTGGAG 
AGTGAATCCT CTGTGGAGAG CTCCCCAGAG CTCAGCCGGA AACCCAGTGT ACAAGAGGCC 
GATCGTCCTC CTTCGTTGGT CTCCTCTCCC TGCATCAGGG CTCCCAGTTC AGACGGACTC 
CCGGCTGAAG CCAAAGTGTC GGTTCCTGAG AGTCCACAGC CTCCGGTCGC CGACTCTGTG 
GACTGTGGAG AGGACAGGAC CGATGGTGGG GTTGTAGAAG GCAGTAGTGA GCCTTCCTCG 
TCCGCAGAAA CATGTGCTTC CTCTTTTCCT GGCTGGATAA AAAGCCCAGA CAGAGGCCCG 
ACTGGACCAG CTGGCCTGAA CTTCTCTCCA GTCAACTCAA ACCTAAGGGA CCTCACCCCC 
TCACACACTC TGGAGCCCCT GGCGGCTCCC TTCAGGCCCG ACGCTGCAGC TGGGGCTGCG 
GCAGGGTCCA CAGCAGGGAC CGGGTCATTG GTTGGCCCTC AAGCCCCCTT CAGTGAAGGC 
CAGGGGCAGC TGTTTTACCC CTGCTCTGAG GAGGGAGGTT CACTCGGCTT CTCGCGCTCA 
CTGAATGGGG ATGGCAGCGG TGAAGGAGGA GGATCAGCGC AGAACCCCCC ACAGAAGAAA 
AAGGTTTCCC TGCTAGAATA CAGGAAACGT CAGCGCGAAG CCCGTCGCAG CGGCTCCAAG 
ACCGAGTGCA GCTCTCCCGT CTCCACCGTG CCACCTCTGA CCGTGGACGC CTTCCCCGTC 
GCGCTGGAGA CGACAAGCGA ACCTCCACTA CCCCCTGCTC CTACGGCTGT CTGCAACGCC 
AACAACACCA TCGTTGTTAA AGAGCCCCAG ACGAGCGAGG AGGCCGAGAC GCCCGGAGAG 
AAAGGCGAGA AGGAAGGAGG AGAAGGACAG TGGACGTCGT CCACTTCTGT AGAGCAGGCC 
CGAGAACGCA GCTACCACAG AGCTCTGCTG CTCAGCAAAG ACAAGGACAC GGATGGTGAG 
ACAGAAGGTG GAGATACACC TGCACTGAGA GATTGCCCGT CTCCAAGTCT TCAGAAGACC 
CCGACTCACA CCCCCTGCTC TCCTGGTCCT CTCGCTCAGC CCACCAATCG GCCACCGAAG 
GAGGAAGACG GCGACGGGCA GCCGAGCAAG CCTGCGGGAC CCAAGTCGGC TCCTCTGACC 
CCCACAAAGC TGCATCCCAC CCCGTTACCC TCCTCGCCGG TCCACTATCC GGGACCCTCC 
CTCCTGCACT CGCCCAAGCC TCAGCCTCAG GGCTCGCCGT ACCGCAGCCA GAGGGCGCTG 
TTCTCCGCTC AGCCTCAAAA CCAACCGCAG CCCCAGGCTC AGACTGGCCC AGCTCCTTTC 
CCTCAGTATA ACACACAGAG TGCTCCACCG CCACCGCCCC CTCCTCCACC AGCACCACCG 
GCCTCCACGG CGTATTTTCC CAGCCAGAGC CCCTCGCCGG CTGGACCTTT CCCCGGGTTC 
AAACCCGCCG TCACGCCCCC TTACCCTCCG TGTTCTCAGC CCCTGATGCA GACTCTTCCC 
CACAGCGTGC ACTATCAGAG CTCAGCCGCT CCACCGCCGC CTCCTCCTCC GCCCCCGCAC 
CCGATGCCCG GCCCCACCCT GCTGCACGTC AACCTGCAGC CCCCTCCCAT CCAGCAGCAC 
CAGCTCATGC TGACCACCGC CCCTCCGCCG CCTCCTCCCC CACAGGGCCA GACCTCACAG 
CAGCAGCAGC AGCAGCCTCC CGCTGGCAGC TCCCTGCTGT CGCTCACCCC TCCTCCGCCG 
CCTCCTCCAC CTCCTCCAGC CCCCTCGACC AACGCCCCGA TGCAGGCCCA CCACTTTCAG 
AACTTAGGGG GTTTTCAGCC GGCGTTGCTA CACCCGGGCG GCGCCGCCAC AAACCCTTCA 
GTGCCCCAAT CCACGTCCCC CCCACCCCTT CAGCAGACCG GACTGCCTCC ACCTCCCCCT 
CCTCCTCCCC AACAGACTCA ACAAGGCCAG GCTCAGGCCG CCGCCTCCCA GATGCCTTCT 
GGGACTCGTG GAGCTCCTGC GTCCTCGACC CCCTTCCACA GCTCGGGGTA CCTGAGCACA 
GGGTGGCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010737212.2
CDS	492..5483
Translation	MSIVIPVGVDTADTSYLDMAAGSDRPESVEASPVVVEKSSYPHQIYSSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPSMLIRQGEGGLFVPGGQDEASRGTTLSTSEDGSYGADITRCICGFTHDDGYMICCDKCSAWQHIDCMGIDRQNIPETYLCERCQPRHLDRERAILLQTRKRECLSDGDTSATESGDEVPLELYSTFQHTPTTITLTTARLGNKQADKKRKKSGDKEPPASSARAKKAFREGSRKSSRVKGAAPEQEPTESLWENKIKTWMERYEEASSNQYSEDVQVLLRVKEQGDGKSLAYNTHPASFKPPVESQVQKNKKILKAVRDLAPDSLIIEYRGKFMLRQQFEANGYFFKRPYPFVLFYSKFDGLEMCVDARSFGNEARFIRRSCTPNSEVRHVIEDGMLHLYIYSLRPITKGTEITIGFDFDYGSCKYKVDCACVKGNQECPVLKHNLEPTENLVGRRRGSRKDKETVRDDLGQNQNMGLDGEGKGKSVGDGKQRKLSPLRLSISNNQDPELYEDLEDKTSVSNEVEMESEEQIAERRRKMANPAEQSHLPVGAASSWKELKHKETREERKMEAILQAFARMEKREKRREQALERIGSIKSEVGGRSDIKEEPPATPETADSPAVMQPLLEVKEEPGLKPAKVKGSRNRKSFSRNRTHIGQQRRRARTISTCSDLQPGSPIESVEPLSNEAPEGETPTAPEPEAISSQAPDTSPPHSSSPAPDRNRTGSKNFKTKKHFVSEWVGEKQQDRGAVRTPEPAPERPLRISSDPEVLATQLNALPGMACSPQVYSTPKHYVRFSSPFLANRSPTTPGVPTGRRRSRELPETPPTTGSCKKRWLKQALEEEGSTSPARRPSLLMPCEGPLSPSINGDSDSPLPYNGTCSLPELPTPLKKRRLSPLDACMSESSTPYGSPCATPTRADQSETPATPILLATPPRPRTEEPSTEPPPNTPTQTLNAPLESESSVESSPELSRKPSVQEADRPPSLVSSPCIRAPSSDGLPAEAKVSVPESPQPPVADSVDCGEDRTDGGVVEGSSEPSSSAETCASSFPGWIKSPDRGPTGPAGLNFSPVNSNLRDLTPSHTLEPLAAPFRPDAAAGAAAGSTAGTGSLVGPQAPFSEGQGQLFYPCSEEGGSLGFSRSLNGDGSGEGGGSAQNPPQKKKVSLLEYRKRQREARRSGSKTECSSPVSTVPPLTVDAFPVALETTSEPPLPPAPTAVCNANNTIVVKEPQTSEEAETPGEKGEKEGGEGQWTSSTSVEQARERSYHRALLLSKDKDTDGETEGGDTPALRDCPSPSLQKTPTHTPCSPGPLAQPTNRPPKEEDGDGQPSKPAGPKSAPLTPTKLHPTPLPSSPVHYPGPSLLHSPKPQPQGSPYRSQRALFSAQPQNQPQPQAQTGPAPFPQYNTQSAPPPPPPPPPAPPASTAYFPSQSPSPAGPFPGFKPAVTPPYPPCSQPLMQTLPHSVHYQSSAAPPPPPPPPPHPMPGPTLLHVNLQPPPIQQHQLMLTTAPPPPPPPQGQTSQQQQQQPPAGSSLLSLTPPPPPPPPPPAPSTNAPMQAHHFQNLGGFQPALLHPGGAATNPSVPQSTSPPPLQQTGLPPPPPPPPQQTQQGQAQAAASQMPSGTRGAPASSTPFHSSGYLSTGWH

Target ORF information:

RefSeq Version	XM_010738910.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010738910.2

ORF Insert Sequence:

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACAGCAGACA CCTCATACCT GGACATGGCT 
GCGGGCTCAG ACAGACCAGA ATCGGTGGAG GCCAGCCCTG TGGTGGTGGA AAAGTCCAGC 
TACCCACACC AGATCTACAG CAGCAGTTCT CACCATTCCC ACAGTTACAT TGGCCTGCCT 
TACGCTGACC ATAACTATGG GGCGCGGCCC CCACCTACAC CACCAGCCTC CCCTCCCCCC 
TCCATGTTGA TCCGACAAGG AGAGGGGGGG TTGTTTGTTC CAGGGGGCCA GGACGAAGCA 
TCAAGGGGCA CGACGCTCAG CACCTCAGAG GATGGCAGCT ACGGGGCTGA CATCACCCGC 
TGCATCTGTG GCTTCACCCA CGATGACGGC TACATGATCT GCTGTGATAA GTGCAGTGCC 
TGGCAGCATA TAGACTGCAT GGGCATCGAC AGGCAGAACA TTCCTGAGAC CTACCTGTGT 
GAACGCTGCC AACCACGACA CCTGGATCGA GAGAGGGCCA TCCTGCTGCA GACCAGGAAA 
CGGGAATGTT TGTCTGATGG GGACACGAGT GCTACAGAGA GTGGAGATGA GGTGCCGCTA 
GAGCTCTACT CCACCTTCCA ACACACGCCT ACCACCATCA CCCTGACTAC CGCGCGCCTT 
GGCAATAAGC AGGCTGATAA GAAACGCAAA AAGAGCGGTG ATAAAGAGCC TCCAGCGTCT 
TCTGCCCGAG CAAAGAAGGC CTTCCGAGAA GGGTCCAGAA AGTCCTCCAG AGTAAAGGGT 
GCAGCGCCAG AGCAGGAGCC AACAGAGTCC CTGTGGGAGA ACAAGATCAA GACGTGGATG 
GAGCGTTATG AGGAGGCCAG CAGCAATCAG TACAGCGAGG ATGTCCAGGT CCTGTTGCGT 
GTCAAAGAAC AAGGCGATGG CAAGAGCCTG GCGTACAACA CGCACCCTGC CTCTTTCAAA 
CCCCCTGTGG AGAGTCAAGT TCAGAAGAAC AAGAAGATCC TCAAAGCGGT GCGAGACTTG 
GCCCCAGACT CCCTCATCAT TGAGTACAGG GGCAAGTTCA TGCTCAGACA GCAGTTTGAG 
GCCAACGGAT ACTTCTTTAA GAGGCCATAC CCATTTGTGT TATTTTATTC TAAATTTGAC 
GGACTGGAGA TGTGCGTGGA CGCTCGTAGC TTCGGCAACG AAGCGCGCTT CATCCGTCGC 
TCCTGCACCC CTAATTCTGA AGTGCGGCAC GTCATTGAGG ACGGCATGCT GCATTTATAC 
ATCTACTCAT TGAGGCCGAT CACCAAGGGC ACAGAGATCA CCATCGGTTT TGATTTTGAC 
TATGGCAGCT GTAAATACAA GGTGGACTGT GCCTGCGTGA AGGGGAATCA GGAGTGCCCG 
GTGCTCAAGC ACAACCTCGA ACCCACAGAG AACCTGGTCG GCCGGCGCAG AGGGAGTCGT 
AAGGACAAGG AGACGGTTCG AGACGACCTG GGCCAGAACC AGAACATGGG TCTGGACGGC 
GAGGGGAAGG GCAAGAGCGT GGGCGACGGC AAACAGAGGA AGCTCTCTCC TCTTCGCCTC 
TCTATCTCAA ACAACCAGGA TCCTGAGTTA TATGAGGATC TAGAAGACAA AACCTCCGTT 
AGCAATGAAG TAGAGATGGA GTCAGAGGAG CAGATTGCAG AGAGGAGGAG GAAGATGGCC 
AACCCAGCGG AGCAGTCCCA TCTCCCTGTC GGGGCGGCTT CCAGCTGGAA AGAACTGAAA 
CACAAGGAGA CACGAGAGGA GAGGAAGATG GAGGCCATCC TGCAAGCGTT TGCCCGAATG 
GAGAAGAGAG AGAAGCGAAG GGAGCAGGCG CTGGAAAGAA TCGGTAGCAT CAAGTCGGAG 
GTCGGGGGAC GCAGTGACAT CAAGGAGGAG CCTCCAGCCA CGCCAGAGAC GGCAGATTCT 
CCAGCTGTTA TGCAGCCGCT CCTGGAGGTG AAAGAGGAGC CAGGATTAAA GCCGGCCAAG 
GTCAAAGGCT CGAGGAACAG GAAAAGTTTC TCAAGGAACC GCACGCACAT TGGCCAACAG 
CGCCGGAGAG CTCGCACCAT CAGCACCTGC TCTGACCTGC AGCCTGGCTC TCCAATTGAA 
TCTGTGGAGC CTCTGAGCAA CGAAGCCCCC GAAGGAGAGA CACCCACCGC ACCAGAGCCG 
GAGGCAATCT CTTCCCAAGC ACCAGACACC AGCCCTCCGC ACAGCAGCTC ACCTGCACCT 
GACAGAAACC GCACCGGGAG CAAGAACTTC AAAACTAAAA AGCACTTTGT GAGTGAGTGG 
GTGGGAGAGA AGCAGCAGGA CCGTGGTGCA GTACGAACCC CAGAGCCGGC CCCCGAGAGG 
CCGCTGAGAA TAAGCAGTGA CCCCGAAGTA CTCGCCACAC AGCTGAACGC CCTCCCAGGC 
ATGGCCTGCT CTCCACAGGT CTACAGCACG CCCAAACACT ACGTCCGCTT CTCGTCCCCT 
TTCCTGGCGA ACCGCAGTCC CACAACTCCC GGAGTCCCCA CTGGGAGACG GCGTTCCAGA 
GAACTGCCAG AAACGCCGCC AACCACCGGC TCCTGCAAGA AGCGATGGTT GAAGCAGGCC 
CTTGAGGAAG AGGGATCCAC CAGCCCAGCC AGACGACCGA GCCTCCTCAT GCCCTGTGAG 
GGTCCTCTTA GCCCCTCCAT CAACGGAGAC TCTGACAGCC CTCTTCCCTA CAATGGCACC 
TGCTCGCTAC CTGAGTTGCC CACGCCTTTG AAAAAGCGCC GGTTGAGTCC ATTAGATGCC 
TGTATGTCTG AGAGCTCCAC ACCCTACGGC TCCCCTTGTG CCACGCCCAC CAGGGCAGAC 
CAATCGGAGA CACCCGCAAC CCCCATCTTA CTGGCTACCC CACCGCGTCC CAGAACGGAG 
GAGCCCAGTA CAGAGCCCCC GCCCAACACC CCAACGCAGA CACTTAACGC CCCTCTGGAG 
AGTGAATCCT CTGTGGAGAG CTCCCCAGAG CTCAGCCGGA AACCCAGTGT ACAAGAGGCC 
GATCGTCCTC CTTCGTTGGT CTCCTCTCCC TGCATCAGGG CTCCCAGTTC AGACGGACTC 
CCGGCTGAAG CCAAAGTGTC GGTTCCTGAG AGTCCACAGC CTCCGGTCGC CGACTCTGTG 
GACTGTGGAG AGGACAGGAC CGATGGTGGG GTTGTAGAAG GCAGTAGTGA GCCTTCCTCG 
TCCGCAGAAA CATGTGCTTC CTCTTTTCCT GGCTGGATAA AAAGCCCAGA CAGAGGCCCG 
ACTGGACCAG CTGGCCTGAA CTTCTCTCCA GTCAACTCAA ACCTAAGGGA CCTCACCCCC 
TCACACACTC TGGAGCCCCT GGCGGCTCCC TTCAGGCCCG ACGCTGCAGC TGGGGCTGCG 
GCAGGGTCCA CAGCAGGGAC CGGGTCATTG GTTGGCCCTC AAGCCCCCTT CAGTGAAGGC 
CAGGGGCAGC TGTTTTACCC CTGCTCTGAG GAGGGAGGTT CACTCGGCTT CTCGCGCTCA 
CTGAATGGGG ATGGCAGCGG TGAAGGAGGA GGATCAGCGC AGAACCCCCC ACAGAAGAAA 
AAGGTTTCCC TGCTAGAATA CAGGAAACGT CAGCGCGAAG CCCGTCGCAG CGGCTCCAAG 
ACCGAGTGCA GCTCTCCCGT CTCCACCGTG CCACCTCTGA CCGTGGACGC CTTCCCCGTC 
GCGCTGGAGA CGACAAGCGA ACCTCCACTA CCCCCTGCTC CTACGGCTGT CTGCAACGCC 
AACAACACCA TCGTTGTTAA AGAGCCCCAG ACGAGCGAGG AGGCCGAGAC GCCCGGAGAG 
AAAGGCGAGA AGGAAGGAGG AGAAGGACAG TGGACGTCGT CCACTTCTGT AGAGCAGGCC 
CGAGAACGCA GCTACCACAG AGCTCTGCTG CTCAGCAAAG ACAAGGACAC GGATGGTGAG 
ACAGAAGGTG GAGATACACC TGCACTGAGA GATTGCCCGT CTCCAAGTCT TCAGAAGACC 
CCGACTCACA CCCCCTGCTC TCCTGGTCCT CTCGCTCAGC CCACCAATCG GCCACCGAAG 
GAGGAAGACG GCGACGGGCA GCCGAGCAAG CCTGCGGGAC CCAAGTCGGC TCCTCTGACC 
CCCACAAAGC TGCATCCCAC CCCGTTACCC TCCTCGCCGG TCCACTATCC GGGACCCTCC 
CTCCTGCACT CGCCCAAGCC TCAGCCTCAG GGCTCGCCGT ACCGCAGCCA GAGGGCGCTG 
TTCTCCGCTC AGCCTCAAAA CCAACCGCAG CCCCAGGCTC AGACTGGCCC AGCTCCTTTC 
CCTCAGTATA ACACACAGAG TGCTCCACCG CCACCGCCCC CTCCTCCACC AGCACCACCG 
GCCTCCACGG CGTATTTTCC CAGCCAGAGC CCCTCGCCGG CTGGACCTTT CCCCGGGTTC 
AAACCCGCCG TCACGCCCCC TTACCCTCCG TGTTCTCAGC CCCTGATGCA GACTCTTCCC 
CACAGCGTGC ACTATCAGAG CTCAGCCGCT CCACCGCCGC CTCCTCCTCC GCCCCCGCAC 
CCGATGCCCG GCCCCACCCT GCTGCACGTC AACCTGCAGC CCCCTCCCAT CCAGCAGCAC 
CAGCTCATGC TGACCACCGC CCCTCCGCCG CCTCCTCCCC CACAGGGCCA GACCTCACAG 
CAGCAGCAGC AGCAGCCTCC CGCTGGCAGC TCCCTGCTGT CGCTCACCCC TCCTCCGCCG 
CCTCCTCCAC CTCCTCCAGC CCCCTCGACC AACGCCCCGA TGCAGGCCCA CCACTTTCAG 
AACTTAGGGG GTTTTCAGCC GGCGTTGCTA CACCCGGGCG GCGCCGCCAC AAACCCTTCA 
GTGCCCCAAT CCACGTCCCC CCCACCCCTT CAGCAGACCG GACTGCCTCC ACCTCCCCCT 
CCTCCTCCCC AACAGACTCA ACAAGGCCAG GCTCAGGCCG CCGCCTCCCA GATGCCTTCT 
GGGACTCGTG GAGCTCCTGC GTCCTCGACC CCCTTCCACA GCTCGGGGTA CCTGAGCACA 
GGGTGGCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kmt2e ( XM_019278449.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019133994.1 Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X4, mRNA.

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CloneID	OLa112876
Clone ID Related Accession (Same CDS sequence)	XM_019278449.1
Accession Version	XM_019278449.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	histone-lysine N-methyltransferase 2E isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017609671.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACAGCAGACA CCTCATACCT GGACATGGCT 
GCGGGCTCAG ACAGACCAGA ATCGGTGGAG GCCAGCCCTG TGGTGGTGGA AAAGTCCAGC 
TACCCACACC AGATCTACAG CAGCAGTTCT CACCATTCCC ACAGTTACAT TGGCCTGCCT 
TACGCTGACC ATAACTATGG GGCGCGGCCC CCACCTACAC CACCAGCCTC CCCTCCCCCC 
TCCATGTTGA TCCGACAAGG AGAGGGGGGG TTGTTTGTTC CAGGGGGCCA GGACGAAGCA 
TCAAGGGGCA CGACGCTCAG CACCTCAGAG GATGGCAGCT ACGGGGCTGA CATCACCCGC 
TGCATCTGTG GCTTCACCCA CGATGACGGC TACATGATCT GCTGTGATAA GTGCAGTGCC 
TGGCAGCATA TAGACTGCAT GGGCATCGAC AGGCAGAACA TTCCTGAGAC CTACCTGTGT 
GAACGCTGCC AACCACGACA CCTGGATCGA GAGAGGGCCA TCCTGCTGCA GACCAGGAAA 
CGGGAATGTT TGTCTGATGG GGACACGAGT GCTACAGAGA GTGGAGATGA GGTGCCGCTA 
GAGCTCTACT CCACCTTCCA ACACACGCCT ACCACCATCA CCCTGACTAC CGCGCGCCTT 
GGCAATAAGC AGGCTGATAA GAAACGCAAA AAGAGCGGTG ATAAAGAGCC TCCAGCGTCT 
TCTGCCCGAG CAAAGAAGGC CTTCCGAGAA GGGTCCAGAA AGTCCTCCAG AGTAAAGGGT 
GCAGCGCCAG AGCAGGAGCC AACAGAGTCC CTGTGGGAGA ACAAGATCAA GACGTGGATG 
GAGCGTTATG AGGAGGCCAG CAGCAATCAG TACAGCGAGG ATGTCCAGGT CCTGTTGCGT 
GTCAAAGAAC AAGGCGATGG CAAGAGCCTG GCGTACAACA CGCACCCTGC CTCTTTCAAA 
CCCCCTGTGG AGAGTCAAGT TCAGAAGAAC AAGAAGATCC TCAAAGCGGT GCGAGACTTG 
GCCCCAGACT CCCTCATCAT TGAGTACAGG GGCAAGTTCA TGCTCAGACA GCAGTTTGAG 
GCCAACGGAT ACTTCTTTAA GAGGCCATAC CCATTTGTGT TATTTTATTC TAAATTTGAC 
GGACTGGAGA TGTGCGTGGA CGCTCGTAGC TTCGGCAACG AAGCGCGCTT CATCCGTCGC 
TCCTGCACCC CTAATTCTGA AGTGCGGCAC GTCATTGAGG ACGGCATGCT GCATTTATAC 
ATCTACTCAT TGAGGCCGAT CACCAAGGGC ACAGAGATCA CCATCGGTTT TGATTTTGAC 
TATGGCAGCT GTAAATACAA GGTGGACTGT GCCTGCGTGA AGGGGAATCA GGAGTGCCCG 
GTGCTCAAGC ACAACCTCGA ACCCACAGAG AACCTGGTCG GCCGGCGCAG AGGGAGTCGT 
AAGGACAAGG AGACGGTTCG AGACGACCTG GGCCAGAACC AGAACATGGG TCTGGACGGC 
GAGGGGAAGG GCAAGAGCGT GGGCGACGGC AAACAGAGGA AGCTCTCTCC TCTTCGCCTC 
TCTATCTCAA ACAACCAGGC CAACCCAGCG GAGCAGTCCC ATCTCCCTGT CGGGGCGGCT 
TCCAGCTGGA AAGAACTGAA ACACAAGGAG GTAAACTTTA CTCCACCCAT CTACCAGCCT 
CCCCACCACC ACCTCTCCCC CAAATCTCCC AGAGAGACAC GAGAGGAGAG GAAGATGGAG 
GCCATCCTGC AAGCGTTTGC CCGAATGGAG AAGAGAGAGA AGCGAAGGGA GCAGGCGCTG 
GAAAGAATCG GTAGCATCAA GTCGGAGGTC GGGGGACGCA GTGACATCAA GGAGGAGCCT 
CCAGCCACGC CAGAGACGGC AGATTCTCCA GCTGTTATGC AGCCGCTCCT GGAGGTGAAA 
GAGGAGCCAG GATTAAAGCC GGCCAAGGTC AAAGGCTCGA GGAACAGGAA AAGTTTCTCA 
AGGAACCGCA CGCACATTGG CCAACAGCGC CGGAGAGCTC GCACCATCAG CACCTGCTCT 
GACCTGCAGC CTGGCTCTCC AATTGAATCT GTGGAGCCTC TGAGCAACGA AGCCCCCGAA 
GGAGAGACAC CCACCGCACC AGAGCCGGAG GCAATCTCTT CCCAAGCACC AGACACCAGC 
CCTCCGCACA GCAGCTCACC TGCACCTGAC AGAAACCGCA CCGGGAGCAA GAACTTCAAA 
ACTAAAAAGC ACTTTGTGAG TGAGTGGGTG GGAGAGAAGC AGCAGGACCG TGGTGCAGTA 
CGAACCCCAG AGCCGGCCCC CGAGAGGCCG CTGAGAATAA GCAGTGACCC CGAAGTACTC 
GCCACACAGC TGAACGCCCT CCCAGGCATG GCCTGCTCTC CACAGGTCTA CAGCACGCCC 
AAACACTACG TCCGCTTCTC GTCCCCTTTC CTGGCGAACC GCAGTCCCAC AACTCCCGGA 
GTCCCCACTG GGAGACGGCG TTCCAGAGAA CTGCCAGAAA CGCCGCCAAC CACCGGCTCC 
TGCAAGAAGC GATGGTTGAA GCAGGCCCTT GAGGAAGAGG GATCCACCAG CCCAGCCAGA 
CGACCGAGCC TCCTCATGCC CTGTGAGGGT CCTCTTAGCC CCTCCATCAA CGGAGACTCT 
GACAGCCCTC TTCCCTACAA TGGCACCTGC TCGCTACCTG AGTTGCCCAC GCCTTTGAAA 
AAGCGCCGGT TGAGTCCATT AGATGCCTGT ATGTCTGAGA GCTCCACACC CTACGGCTCC 
CCTTGTGCCA CGCCCACCAG GGCAGACCAA TCGGAGACAC CCGCAACCCC CATCTTACTG 
GCTACCCCAC CGCGTCCCAG AACGGAGGAG CCCAGTACAG AGCCCCCGCC CAACACCCCA 
ACGCAGACAC TTAACGCCCC TCTGGAGAGT GAATCCTCTG TGGAGAGCTC CCCAGAGCTC 
AGCCGGAAAC CCAGTGTACA AGAGGCCGAT CGTCCTCCTT CGTTGGTCTC CTCTCCCTGC 
ATCAGGGCTC CCAGTTCAGA CGGACTCCCG GCTGAAGCCA AAGTGTCGGT TCCTGAGAGT 
CCACAGCCTC CGGTCGCCGA CTCTGTGGAC TGTGGAGAGG ACAGGACCGA TGGTGGGGTT 
GTAGAAGGCA GTAGTGAGCC TTCCTCGTCC GCAGAAACAT GTGCTTCCTC TTTTCCTGGC 
TGGATAAAAA GCCCAGACAG AGGCCCGACT GGACCAGCTG GCCTGAACTT CTCTCCAGTC 
AACTCAAACC TAAGGGACCT CACCCCCTCA CACACTCTGG AGCCCCTGGC GGCTCCCTTC 
AGGCCCGACG CTGCAGCTGG GGCTGCGGCA GGGTCCACAG CAGGGACCGG GTCATTGGTT 
GGCCCTCAAG CCCCCTTCAG TGAAGGCCAG GGGCAGCTGT TTTACCCCTG CTCTGAGGAG 
GGAGGTTCAC TCGGCTTCTC GCGCTCACTG AATGGGGATG GCAGCGGTGA AGGAGGAGGA 
TCAGCGCAGA ACCCCCCACA GAAGAAAAAG GTTTCCCTGC TAGAATACAG GAAACGTCAG 
CGCGAAGCCC GTCGCAGCGG CTCCAAGACC GAGTGCAGCT CTCCCGTCTC CACCGTGCCA 
CCTCTGACCG TGGACGCCTT CCCCGTCGCG CTGGAGACGA CAAGCGAACC TCCACTACCC 
CCTGCTCCTA CGGCTGTCTG CAACGCCAAC AACACCATCG TTGTTAAAGA GCCCCAGACG 
AGCGAGGAGG CCGAGACGCC CGGAGAGAAA GGCGAGAAGG AAGGAGGAGA AGGACAGTGG 
ACGTCGTCCA CTTCTGTAGA GCAGGCCCGA GAACGCAGCT ACCACAGAGC TCTGCTGCTC 
AGCAAAGACA AGGACACGGA TGGTGAGACA GAAGGTGGAG ATACACCTGC ACTGAGAGAT 
TGCCCGTCTC CAAGTCTTCA GAAGACCCCG ACTCACACCC CCTGCTCTCC TGGTCCTCTC 
GCTCAGCCCA CCAATCGGCC ACCGAAGGAG GAAGACGGCG ACGGGCAGCC GAGCAAGCCT 
GCGGGACCCA AGTCGGCTCC TCTGACCCCC ACAAAGCTGC ATCCCACCCC GTTACCCTCC 
TCGCCGGTCC ACTATCCGGG ACCCTCCCTC CTGCACTCGC CCAAGCCTCA GCCTCAGGGC 
TCGCCGTACC GCAGCCAGAG GGCGCTGTTC TCCGCTCAGC CTCAAAACCA ACCGCAGCCC 
CAGGCTCAGA CTGGCCCAGC TCCTTTCCCT CAGTATAACA CACAGAGTGC TCCACCGCCA 
CCGCCCCCTC CTCCACCAGC ACCACCGGCC TCCACGGCGT ATTTTCCCAG CCAGAGCCCC 
TCGCCGGCTG GACCTTTCCC CGGGTTCAAA CCCGCCGTCA CGCCCCCTTA CCCTCCGTGT 
TCTCAGCCCC TGATGCAGAC TCTTCCCCAC AGCGTGCACT ATCAGAGCTC AGCCGCTCCA 
CCGCCGCCTC CTCCTCCGCC CCCGCACCCG ATGCCCGGCC CCACCCTGCT GCACGTCAAC 
CTGCAGCCCC CTCCCATCCA GCAGCACCAG CTCATGCTGA CCACCGCCCC TCCGCCGCCT 
CCTCCCCCAC AGGGCCAGAC CTCACAGCAG CAGCAGCAGC AGCCTCCCGC TGGCAGCTCC 
CTGCTGTCGC TCACCCCTCC TCCGCCGCCT CCTCCACCTC CTCCAGCCCC CTCGACCAAC 
GCCCCGATGC AGGCCCACCA CTTTCAGAAC TTAGGGGGTT TTCAGCCGGC GTTGCTACAC 
CCGGGCGGCG CCGCCACAAA CCCTTCAGTG CCCCAATCCA CGTCCCCCCC ACCCCTTCAG 
CAGACCGGAC TGCCTCCACC TCCCCCTCCT CCTCCCCAAC AGACTCAACA AGGCCAGGCT 
CAGGCCGCCG CCTCCCAGAT GCCTTCTGGG ACTCGTGGAG CTCCTGCGTC CTCGACCCCC 
TTCCACAGCT CGGGGTACCT GAGCACAGGG TGGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019133994.1
CDS	492..5450
Translation	MSIVIPVGVDTADTSYLDMAAGSDRPESVEASPVVVEKSSYPHQIYSSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPSMLIRQGEGGLFVPGGQDEASRGTTLSTSEDGSYGADITRCICGFTHDDGYMICCDKCSAWQHIDCMGIDRQNIPETYLCERCQPRHLDRERAILLQTRKRECLSDGDTSATESGDEVPLELYSTFQHTPTTITLTTARLGNKQADKKRKKSGDKEPPASSARAKKAFREGSRKSSRVKGAAPEQEPTESLWENKIKTWMERYEEASSNQYSEDVQVLLRVKEQGDGKSLAYNTHPASFKPPVESQVQKNKKILKAVRDLAPDSLIIEYRGKFMLRQQFEANGYFFKRPYPFVLFYSKFDGLEMCVDARSFGNEARFIRRSCTPNSEVRHVIEDGMLHLYIYSLRPITKGTEITIGFDFDYGSCKYKVDCACVKGNQECPVLKHNLEPTENLVGRRRGSRKDKETVRDDLGQNQNMGLDGEGKGKSVGDGKQRKLSPLRLSISNNQANPAEQSHLPVGAASSWKELKHKEVNFTPPIYQPPHHHLSPKSPRETREERKMEAILQAFARMEKREKRREQALERIGSIKSEVGGRSDIKEEPPATPETADSPAVMQPLLEVKEEPGLKPAKVKGSRNRKSFSRNRTHIGQQRRRARTISTCSDLQPGSPIESVEPLSNEAPEGETPTAPEPEAISSQAPDTSPPHSSSPAPDRNRTGSKNFKTKKHFVSEWVGEKQQDRGAVRTPEPAPERPLRISSDPEVLATQLNALPGMACSPQVYSTPKHYVRFSSPFLANRSPTTPGVPTGRRRSRELPETPPTTGSCKKRWLKQALEEEGSTSPARRPSLLMPCEGPLSPSINGDSDSPLPYNGTCSLPELPTPLKKRRLSPLDACMSESSTPYGSPCATPTRADQSETPATPILLATPPRPRTEEPSTEPPPNTPTQTLNAPLESESSVESSPELSRKPSVQEADRPPSLVSSPCIRAPSSDGLPAEAKVSVPESPQPPVADSVDCGEDRTDGGVVEGSSEPSSSAETCASSFPGWIKSPDRGPTGPAGLNFSPVNSNLRDLTPSHTLEPLAAPFRPDAAAGAAAGSTAGTGSLVGPQAPFSEGQGQLFYPCSEEGGSLGFSRSLNGDGSGEGGGSAQNPPQKKKVSLLEYRKRQREARRSGSKTECSSPVSTVPPLTVDAFPVALETTSEPPLPPAPTAVCNANNTIVVKEPQTSEEAETPGEKGEKEGGEGQWTSSTSVEQARERSYHRALLLSKDKDTDGETEGGDTPALRDCPSPSLQKTPTHTPCSPGPLAQPTNRPPKEEDGDGQPSKPAGPKSAPLTPTKLHPTPLPSSPVHYPGPSLLHSPKPQPQGSPYRSQRALFSAQPQNQPQPQAQTGPAPFPQYNTQSAPPPPPPPPPAPPASTAYFPSQSPSPAGPFPGFKPAVTPPYPPCSQPLMQTLPHSVHYQSSAAPPPPPPPPPHPMPGPTLLHVNLQPPPIQQHQLMLTTAPPPPPPPQGQTSQQQQQQPPAGSSLLSLTPPPPPPPPPPAPSTNAPMQAHHFQNLGGFQPALLHPGGAATNPSVPQSTSPPPLQQTGLPPPPPPPPQQTQQGQAQAAASQMPSGTRGAPASSTPFHSSGYLSTGWH

Target ORF information:

RefSeq Version	XM_019278449.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019278449.1

ORF Insert Sequence:

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACAGCAGACA CCTCATACCT GGACATGGCT 
GCGGGCTCAG ACAGACCAGA ATCGGTGGAG GCCAGCCCTG TGGTGGTGGA AAAGTCCAGC 
TACCCACACC AGATCTACAG CAGCAGTTCT CACCATTCCC ACAGTTACAT TGGCCTGCCT 
TACGCTGACC ATAACTATGG GGCGCGGCCC CCACCTACAC CACCAGCCTC CCCTCCCCCC 
TCCATGTTGA TCCGACAAGG AGAGGGGGGG TTGTTTGTTC CAGGGGGCCA GGACGAAGCA 
TCAAGGGGCA CGACGCTCAG CACCTCAGAG GATGGCAGCT ACGGGGCTGA CATCACCCGC 
TGCATCTGTG GCTTCACCCA CGATGACGGC TACATGATCT GCTGTGATAA GTGCAGTGCC 
TGGCAGCATA TAGACTGCAT GGGCATCGAC AGGCAGAACA TTCCTGAGAC CTACCTGTGT 
GAACGCTGCC AACCACGACA CCTGGATCGA GAGAGGGCCA TCCTGCTGCA GACCAGGAAA 
CGGGAATGTT TGTCTGATGG GGACACGAGT GCTACAGAGA GTGGAGATGA GGTGCCGCTA 
GAGCTCTACT CCACCTTCCA ACACACGCCT ACCACCATCA CCCTGACTAC CGCGCGCCTT 
GGCAATAAGC AGGCTGATAA GAAACGCAAA AAGAGCGGTG ATAAAGAGCC TCCAGCGTCT 
TCTGCCCGAG CAAAGAAGGC CTTCCGAGAA GGGTCCAGAA AGTCCTCCAG AGTAAAGGGT 
GCAGCGCCAG AGCAGGAGCC AACAGAGTCC CTGTGGGAGA ACAAGATCAA GACGTGGATG 
GAGCGTTATG AGGAGGCCAG CAGCAATCAG TACAGCGAGG ATGTCCAGGT CCTGTTGCGT 
GTCAAAGAAC AAGGCGATGG CAAGAGCCTG GCGTACAACA CGCACCCTGC CTCTTTCAAA 
CCCCCTGTGG AGAGTCAAGT TCAGAAGAAC AAGAAGATCC TCAAAGCGGT GCGAGACTTG 
GCCCCAGACT CCCTCATCAT TGAGTACAGG GGCAAGTTCA TGCTCAGACA GCAGTTTGAG 
GCCAACGGAT ACTTCTTTAA GAGGCCATAC CCATTTGTGT TATTTTATTC TAAATTTGAC 
GGACTGGAGA TGTGCGTGGA CGCTCGTAGC TTCGGCAACG AAGCGCGCTT CATCCGTCGC 
TCCTGCACCC CTAATTCTGA AGTGCGGCAC GTCATTGAGG ACGGCATGCT GCATTTATAC 
ATCTACTCAT TGAGGCCGAT CACCAAGGGC ACAGAGATCA CCATCGGTTT TGATTTTGAC 
TATGGCAGCT GTAAATACAA GGTGGACTGT GCCTGCGTGA AGGGGAATCA GGAGTGCCCG 
GTGCTCAAGC ACAACCTCGA ACCCACAGAG AACCTGGTCG GCCGGCGCAG AGGGAGTCGT 
AAGGACAAGG AGACGGTTCG AGACGACCTG GGCCAGAACC AGAACATGGG TCTGGACGGC 
GAGGGGAAGG GCAAGAGCGT GGGCGACGGC AAACAGAGGA AGCTCTCTCC TCTTCGCCTC 
TCTATCTCAA ACAACCAGGC CAACCCAGCG GAGCAGTCCC ATCTCCCTGT CGGGGCGGCT 
TCCAGCTGGA AAGAACTGAA ACACAAGGAG GTAAACTTTA CTCCACCCAT CTACCAGCCT 
CCCCACCACC ACCTCTCCCC CAAATCTCCC AGAGAGACAC GAGAGGAGAG GAAGATGGAG 
GCCATCCTGC AAGCGTTTGC CCGAATGGAG AAGAGAGAGA AGCGAAGGGA GCAGGCGCTG 
GAAAGAATCG GTAGCATCAA GTCGGAGGTC GGGGGACGCA GTGACATCAA GGAGGAGCCT 
CCAGCCACGC CAGAGACGGC AGATTCTCCA GCTGTTATGC AGCCGCTCCT GGAGGTGAAA 
GAGGAGCCAG GATTAAAGCC GGCCAAGGTC AAAGGCTCGA GGAACAGGAA AAGTTTCTCA 
AGGAACCGCA CGCACATTGG CCAACAGCGC CGGAGAGCTC GCACCATCAG CACCTGCTCT 
GACCTGCAGC CTGGCTCTCC AATTGAATCT GTGGAGCCTC TGAGCAACGA AGCCCCCGAA 
GGAGAGACAC CCACCGCACC AGAGCCGGAG GCAATCTCTT CCCAAGCACC AGACACCAGC 
CCTCCGCACA GCAGCTCACC TGCACCTGAC AGAAACCGCA CCGGGAGCAA GAACTTCAAA 
ACTAAAAAGC ACTTTGTGAG TGAGTGGGTG GGAGAGAAGC AGCAGGACCG TGGTGCAGTA 
CGAACCCCAG AGCCGGCCCC CGAGAGGCCG CTGAGAATAA GCAGTGACCC CGAAGTACTC 
GCCACACAGC TGAACGCCCT CCCAGGCATG GCCTGCTCTC CACAGGTCTA CAGCACGCCC 
AAACACTACG TCCGCTTCTC GTCCCCTTTC CTGGCGAACC GCAGTCCCAC AACTCCCGGA 
GTCCCCACTG GGAGACGGCG TTCCAGAGAA CTGCCAGAAA CGCCGCCAAC CACCGGCTCC 
TGCAAGAAGC GATGGTTGAA GCAGGCCCTT GAGGAAGAGG GATCCACCAG CCCAGCCAGA 
CGACCGAGCC TCCTCATGCC CTGTGAGGGT CCTCTTAGCC CCTCCATCAA CGGAGACTCT 
GACAGCCCTC TTCCCTACAA TGGCACCTGC TCGCTACCTG AGTTGCCCAC GCCTTTGAAA 
AAGCGCCGGT TGAGTCCATT AGATGCCTGT ATGTCTGAGA GCTCCACACC CTACGGCTCC 
CCTTGTGCCA CGCCCACCAG GGCAGACCAA TCGGAGACAC CCGCAACCCC CATCTTACTG 
GCTACCCCAC CGCGTCCCAG AACGGAGGAG CCCAGTACAG AGCCCCCGCC CAACACCCCA 
ACGCAGACAC TTAACGCCCC TCTGGAGAGT GAATCCTCTG TGGAGAGCTC CCCAGAGCTC 
AGCCGGAAAC CCAGTGTACA AGAGGCCGAT CGTCCTCCTT CGTTGGTCTC CTCTCCCTGC 
ATCAGGGCTC CCAGTTCAGA CGGACTCCCG GCTGAAGCCA AAGTGTCGGT TCCTGAGAGT 
CCACAGCCTC CGGTCGCCGA CTCTGTGGAC TGTGGAGAGG ACAGGACCGA TGGTGGGGTT 
GTAGAAGGCA GTAGTGAGCC TTCCTCGTCC GCAGAAACAT GTGCTTCCTC TTTTCCTGGC 
TGGATAAAAA GCCCAGACAG AGGCCCGACT GGACCAGCTG GCCTGAACTT CTCTCCAGTC 
AACTCAAACC TAAGGGACCT CACCCCCTCA CACACTCTGG AGCCCCTGGC GGCTCCCTTC 
AGGCCCGACG CTGCAGCTGG GGCTGCGGCA GGGTCCACAG CAGGGACCGG GTCATTGGTT 
GGCCCTCAAG CCCCCTTCAG TGAAGGCCAG GGGCAGCTGT TTTACCCCTG CTCTGAGGAG 
GGAGGTTCAC TCGGCTTCTC GCGCTCACTG AATGGGGATG GCAGCGGTGA AGGAGGAGGA 
TCAGCGCAGA ACCCCCCACA GAAGAAAAAG GTTTCCCTGC TAGAATACAG GAAACGTCAG 
CGCGAAGCCC GTCGCAGCGG CTCCAAGACC GAGTGCAGCT CTCCCGTCTC CACCGTGCCA 
CCTCTGACCG TGGACGCCTT CCCCGTCGCG CTGGAGACGA CAAGCGAACC TCCACTACCC 
CCTGCTCCTA CGGCTGTCTG CAACGCCAAC AACACCATCG TTGTTAAAGA GCCCCAGACG 
AGCGAGGAGG CCGAGACGCC CGGAGAGAAA GGCGAGAAGG AAGGAGGAGA AGGACAGTGG 
ACGTCGTCCA CTTCTGTAGA GCAGGCCCGA GAACGCAGCT ACCACAGAGC TCTGCTGCTC 
AGCAAAGACA AGGACACGGA TGGTGAGACA GAAGGTGGAG ATACACCTGC ACTGAGAGAT 
TGCCCGTCTC CAAGTCTTCA GAAGACCCCG ACTCACACCC CCTGCTCTCC TGGTCCTCTC 
GCTCAGCCCA CCAATCGGCC ACCGAAGGAG GAAGACGGCG ACGGGCAGCC GAGCAAGCCT 
GCGGGACCCA AGTCGGCTCC TCTGACCCCC ACAAAGCTGC ATCCCACCCC GTTACCCTCC 
TCGCCGGTCC ACTATCCGGG ACCCTCCCTC CTGCACTCGC CCAAGCCTCA GCCTCAGGGC 
TCGCCGTACC GCAGCCAGAG GGCGCTGTTC TCCGCTCAGC CTCAAAACCA ACCGCAGCCC 
CAGGCTCAGA CTGGCCCAGC TCCTTTCCCT CAGTATAACA CACAGAGTGC TCCACCGCCA 
CCGCCCCCTC CTCCACCAGC ACCACCGGCC TCCACGGCGT ATTTTCCCAG CCAGAGCCCC 
TCGCCGGCTG GACCTTTCCC CGGGTTCAAA CCCGCCGTCA CGCCCCCTTA CCCTCCGTGT 
TCTCAGCCCC TGATGCAGAC TCTTCCCCAC AGCGTGCACT ATCAGAGCTC AGCCGCTCCA 
CCGCCGCCTC CTCCTCCGCC CCCGCACCCG ATGCCCGGCC CCACCCTGCT GCACGTCAAC 
CTGCAGCCCC CTCCCATCCA GCAGCACCAG CTCATGCTGA CCACCGCCCC TCCGCCGCCT 
CCTCCCCCAC AGGGCCAGAC CTCACAGCAG CAGCAGCAGC AGCCTCCCGC TGGCAGCTCC 
CTGCTGTCGC TCACCCCTCC TCCGCCGCCT CCTCCACCTC CTCCAGCCCC CTCGACCAAC 
GCCCCGATGC AGGCCCACCA CTTTCAGAAC TTAGGGGGTT TTCAGCCGGC GTTGCTACAC 
CCGGGCGGCG CCGCCACAAA CCCTTCAGTG CCCCAATCCA CGTCCCCCCC ACCCCTTCAG 
CAGACCGGAC TGCCTCCACC TCCCCCTCCT CCTCCCCAAC AGACTCAACA AGGCCAGGCT 
CAGGCCGCCG CCTCCCAGAT GCCTTCTGGG ACTCGTGGAG CTCCTGCGTC CTCGACCCCC 
TTCCACAGCT CGGGGTACCT GAGCACAGGG TGGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kmt2e ( XM_010738912.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010737214.2 Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X5, mRNA.

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CloneID	OLa112877
Clone ID Related Accession (Same CDS sequence)	XM_010738912.2
Accession Version	XM_010738912.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	histone-lysine N-methyltransferase 2E isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017609671.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 29, 2016 this sequence version replaced XM_010738912.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACAGCAGACA CCTCATACCT GGACATGGCT 
GCGGGCTCAG ACAGACCAGA ATCGGTGGAG GCCAGCCCTG TGGTGGTGGA AAAGTCCAGC 
TACCCACACC AGATCTACAG CAGCAGTTCT CACCATTCCC ACAGTTACAT TGGCCTGCCT 
TACGCTGACC ATAACTATGG GGCGCGGCCC CCACCTACAC CACCAGCCTC CCCTCCCCCC 
TCCATGTTGA TCCGACAAGG AGAGGGGGGG TTGTTTGTTC CAGGGGGCCA GGACGAAGCA 
TCAAGGGGCA CGACGCTCAG CACCTCAGAG GATGGCAGCT ACGGGGCTGA CATCACCCGC 
TGCATCTGTG GCTTCACCCA CGATGACGGC TACATGATCT GCTGTGATAA GTGCAGTGCC 
TGGCAGCATA TAGACTGCAT GGGCATCGAC AGGCAGAACA TTCCTGAGAC CTACCTGTGT 
GAACGCTGCC AACCACGACA CCTGGATCGA GAGAGGGCCA TCCTGCTGCA GACCAGGAAA 
CGGGAATGTT TGTCTGATGG GGACACGAGT GCTACAGAGA GTGGAGATGA GGTGCCGCTA 
GAGCTCTACT CCACCTTCCA ACACACGCCT ACCACCATCA CCCTGACTAC CGCGCGCCTT 
GGCAATAAGC AGGCTGATAA GAAACGCAAA AAGAGCGGTG ATAAAGAGCC TCCAGCGTCT 
TCTGCCCGAG CAAAGAAGGC CTTCCGAGAA GGGTCCAGAA AGTCCTCCAG AGTAAAGGGT 
GCAGCGCCAG AGCAGGAGCC AACAGAGTCC CTGTGGGAGA ACAAGATCAA GACGTGGATG 
GAGCGTTATG AGGAGGCCAG CAGCAATCAG TACAGCGAGG ATGTCCAGGT CCTGTTGCGT 
GTCAAAGAAC AAGGCGATGG CAAGAGCCTG GCGTACAACA CGCACCCTGC CTCTTTCAAA 
CCCCCTGTGG AGAGTCAAGT TCAGAAGAAC AAGAAGATCC TCAAAGCGGT GCGAGACTTG 
GCCCCAGACT CCCTCATCAT TGAGTACAGG GGCAAGTTCA TGCTCAGACA GCAGTTTGAG 
GCCAACGGAT ACTTCTTTAA GAGGCCATAC CCATTTGTGT TATTTTATTC TAAATTTGAC 
GGACTGGAGA TGTGCGTGGA CGCTCGTAGC TTCGGCAACG AAGCGCGCTT CATCCGTCGC 
TCCTGCACCC CTAATTCTGA AGTGCGGCAC GTCATTGAGG ACGGCATGCT GCATTTATAC 
ATCTACTCAT TGAGGCCGAT CACCAAGGGC ACAGAGATCA CCATCGGTTT TGATTTTGAC 
TATGGCAGCT GTAAATACAA GGTGGACTGT GCCTGCGTGA AGGGGAATCA GGAGTGCCCG 
GTGCTCAAGC ACAACCTCGA ACCCACAGAG AACCTGGTCG GCCGGCGCAG AGGGAGTCGT 
AAGGACAAGG AGACGGTTCG AGACGACCTG GGCCAGAACC AGAACATGGG TCTGGACGGC 
GAGGGGAAGG GCAAGAGCGT GGGCGACGGC AAACAGAGGA AGCTCTCTCC TCTTCGCCTC 
TCTATCTCAA ACAACCAGGA TCCTGAGTTA TATGAGGATC TAGAAGACAA AACCTCCGTT 
AGCAATGAAG TAGAGATGGA GTCAGAGGAG CAGATTGCAG AGAGGAGGAG GAAGATGACA 
CGAGAGGAGA GGAAGATGGA GGCCATCCTG CAAGCGTTTG CCCGAATGGA GAAGAGAGAG 
AAGCGAAGGG AGCAGGCGCT GGAAAGAATC GGTAGCATCA AGTCGGAGGT CGGGGGACGC 
AGTGACATCA AGGAGGAGCC TCCAGCCACG CCAGAGACGG CAGATTCTCC AGCTGTTATG 
CAGCCGCTCC TGGAGGTGAA AGAGGAGCCA GGATTAAAGC CGGCCAAGGT CAAAGGCTCG 
AGGAACAGGA AAAGTTTCTC AAGGAACCGC ACGCACATTG GCCAACAGCG CCGGAGAGCT 
CGCACCATCA GCACCTGCTC TGACCTGCAG CCTGGCTCTC CAATTGAATC TGTGGAGCCT 
CTGAGCAACG AAGCCCCCGA AGGAGAGACA CCCACCGCAC CAGAGCCGGA GGCAATCTCT 
TCCCAAGCAC CAGACACCAG CCCTCCGCAC AGCAGCTCAC CTGCACCTGA CAGAAACCGC 
ACCGGGAGCA AGAACTTCAA AACTAAAAAG CACTTTGTGA GTGAGTGGGT GGGAGAGAAG 
CAGCAGGACC GTGGTGCAGT ACGAACCCCA GAGCCGGCCC CCGAGAGGCC GCTGAGAATA 
AGCAGTGACC CCGAAGTACT CGCCACACAG CTGAACGCCC TCCCAGGCAT GGCCTGCTCT 
CCACAGGTCT ACAGCACGCC CAAACACTAC GTCCGCTTCT CGTCCCCTTT CCTGGCGAAC 
CGCAGTCCCA CAACTCCCGG AGTCCCCACT GGGAGACGGC GTTCCAGAGA ACTGCCAGAA 
ACGCCGCCAA CCACCGGCTC CTGCAAGAAG CGATGGTTGA AGCAGGCCCT TGAGGAAGAG 
GGATCCACCA GCCCAGCCAG ACGACCGAGC CTCCTCATGC CCTGTGAGGG TCCTCTTAGC 
CCCTCCATCA ACGGAGACTC TGACAGCCCT CTTCCCTACA ATGGCACCTG CTCGCTACCT 
GAGTTGCCCA CGCCTTTGAA AAAGCGCCGG TTGAGTCCAT TAGATGCCTG TATGTCTGAG 
AGCTCCACAC CCTACGGCTC CCCTTGTGCC ACGCCCACCA GGGCAGACCA ATCGGAGACA 
CCCGCAACCC CCATCTTACT GGCTACCCCA CCGCGTCCCA GAACGGAGGA GCCCAGTACA 
GAGCCCCCGC CCAACACCCC AACGCAGACA CTTAACGCCC CTCTGGAGAG TGAATCCTCT 
GTGGAGAGCT CCCCAGAGCT CAGCCGGAAA CCCAGTGTAC AAGAGGCCGA TCGTCCTCCT 
TCGTTGGTCT CCTCTCCCTG CATCAGGGCT CCCAGTTCAG ACGGACTCCC GGCTGAAGCC 
AAAGTGTCGG TTCCTGAGAG TCCACAGCCT CCGGTCGCCG ACTCTGTGGA CTGTGGAGAG 
GACAGGACCG ATGGTGGGGT TGTAGAAGGC AGTAGTGAGC CTTCCTCGTC CGCAGAAACA 
TGTGCTTCCT CTTTTCCTGG CTGGATAAAA AGCCCAGACA GAGGCCCGAC TGGACCAGCT 
GGCCTGAACT TCTCTCCAGT CAACTCAAAC CTAAGGGACC TCACCCCCTC ACACACTCTG 
GAGCCCCTGG CGGCTCCCTT CAGGCCCGAC GCTGCAGCTG GGGCTGCGGC AGGGTCCACA 
GCAGGGACCG GGTCATTGGT TGGCCCTCAA GCCCCCTTCA GTGAAGGCCA GGGGCAGCTG 
TTTTACCCCT GCTCTGAGGA GGGAGGTTCA CTCGGCTTCT CGCGCTCACT GAATGGGGAT 
GGCAGCGGTG AAGGAGGAGG ATCAGCGCAG AACCCCCCAC AGAAGAAAAA GGTTTCCCTG 
CTAGAATACA GGAAACGTCA GCGCGAAGCC CGTCGCAGCG GCTCCAAGAC CGAGTGCAGC 
TCTCCCGTCT CCACCGTGCC ACCTCTGACC GTGGACGCCT TCCCCGTCGC GCTGGAGACG 
ACAAGCGAAC CTCCACTACC CCCTGCTCCT ACGGCTGTCT GCAACGCCAA CAACACCATC 
GTTGTTAAAG AGCCCCAGAC GAGCGAGGAG GCCGAGACGC CCGGAGAGAA AGGCGAGAAG 
GAAGGAGGAG AAGGACAGTG GACGTCGTCC ACTTCTGTAG AGCAGGCCCG AGAACGCAGC 
TACCACAGAG CTCTGCTGCT CAGCAAAGAC AAGGACACGG ATGGTGAGAC AGAAGGTGGA 
GATACACCTG CACTGAGAGA TTGCCCGTCT CCAAGTCTTC AGAAGACCCC GACTCACACC 
CCCTGCTCTC CTGGTCCTCT CGCTCAGCCC ACCAATCGGC CACCGAAGGA GGAAGACGGC 
GACGGGCAGC CGAGCAAGCC TGCGGGACCC AAGTCGGCTC CTCTGACCCC CACAAAGCTG 
CATCCCACCC CGTTACCCTC CTCGCCGGTC CACTATCCGG GACCCTCCCT CCTGCACTCG 
CCCAAGCCTC AGCCTCAGGG CTCGCCGTAC CGCAGCCAGA GGGCGCTGTT CTCCGCTCAG 
CCTCAAAACC AACCGCAGCC CCAGGCTCAG ACTGGCCCAG CTCCTTTCCC TCAGTATAAC 
ACACAGAGTG CTCCACCGCC ACCGCCCCCT CCTCCACCAG CACCACCGGC CTCCACGGCG 
TATTTTCCCA GCCAGAGCCC CTCGCCGGCT GGACCTTTCC CCGGGTTCAA ACCCGCCGTC 
ACGCCCCCTT ACCCTCCGTG TTCTCAGCCC CTGATGCAGA CTCTTCCCCA CAGCGTGCAC 
TATCAGAGCT CAGCCGCTCC ACCGCCGCCT CCTCCTCCGC CCCCGCACCC GATGCCCGGC 
CCCACCCTGC TGCACGTCAA CCTGCAGCCC CCTCCCATCC AGCAGCACCA GCTCATGCTG 
ACCACCGCCC CTCCGCCGCC TCCTCCCCCA CAGGGCCAGA CCTCACAGCA GCAGCAGCAG 
CAGCCTCCCG CTGGCAGCTC CCTGCTGTCG CTCACCCCTC CTCCGCCGCC TCCTCCACCT 
CCTCCAGCCC CCTCGACCAA CGCCCCGATG CAGGCCCACC ACTTTCAGAA CTTAGGGGGT 
TTTCAGCCGG CGTTGCTACA CCCGGGCGGC GCCGCCACAA ACCCTTCAGT GCCCCAATCC 
ACGTCCCCCC CACCCCTTCA GCAGACCGGA CTGCCTCCAC CTCCCCCTCC TCCTCCCCAA 
CAGACTCAAC AAGGCCAGGC TCAGGCCGCC GCCTCCCAGA TGCCTTCTGG GACTCGTGGA 
GCTCCTGCGT CCTCGACCCC CTTCCACAGC TCGGGGTACC TGAGCACAGG GTGGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010737214.2
CDS	492..5411
Translation	MSIVIPVGVDTADTSYLDMAAGSDRPESVEASPVVVEKSSYPHQIYSSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPSMLIRQGEGGLFVPGGQDEASRGTTLSTSEDGSYGADITRCICGFTHDDGYMICCDKCSAWQHIDCMGIDRQNIPETYLCERCQPRHLDRERAILLQTRKRECLSDGDTSATESGDEVPLELYSTFQHTPTTITLTTARLGNKQADKKRKKSGDKEPPASSARAKKAFREGSRKSSRVKGAAPEQEPTESLWENKIKTWMERYEEASSNQYSEDVQVLLRVKEQGDGKSLAYNTHPASFKPPVESQVQKNKKILKAVRDLAPDSLIIEYRGKFMLRQQFEANGYFFKRPYPFVLFYSKFDGLEMCVDARSFGNEARFIRRSCTPNSEVRHVIEDGMLHLYIYSLRPITKGTEITIGFDFDYGSCKYKVDCACVKGNQECPVLKHNLEPTENLVGRRRGSRKDKETVRDDLGQNQNMGLDGEGKGKSVGDGKQRKLSPLRLSISNNQDPELYEDLEDKTSVSNEVEMESEEQIAERRRKMTREERKMEAILQAFARMEKREKRREQALERIGSIKSEVGGRSDIKEEPPATPETADSPAVMQPLLEVKEEPGLKPAKVKGSRNRKSFSRNRTHIGQQRRRARTISTCSDLQPGSPIESVEPLSNEAPEGETPTAPEPEAISSQAPDTSPPHSSSPAPDRNRTGSKNFKTKKHFVSEWVGEKQQDRGAVRTPEPAPERPLRISSDPEVLATQLNALPGMACSPQVYSTPKHYVRFSSPFLANRSPTTPGVPTGRRRSRELPETPPTTGSCKKRWLKQALEEEGSTSPARRPSLLMPCEGPLSPSINGDSDSPLPYNGTCSLPELPTPLKKRRLSPLDACMSESSTPYGSPCATPTRADQSETPATPILLATPPRPRTEEPSTEPPPNTPTQTLNAPLESESSVESSPELSRKPSVQEADRPPSLVSSPCIRAPSSDGLPAEAKVSVPESPQPPVADSVDCGEDRTDGGVVEGSSEPSSSAETCASSFPGWIKSPDRGPTGPAGLNFSPVNSNLRDLTPSHTLEPLAAPFRPDAAAGAAAGSTAGTGSLVGPQAPFSEGQGQLFYPCSEEGGSLGFSRSLNGDGSGEGGGSAQNPPQKKKVSLLEYRKRQREARRSGSKTECSSPVSTVPPLTVDAFPVALETTSEPPLPPAPTAVCNANNTIVVKEPQTSEEAETPGEKGEKEGGEGQWTSSTSVEQARERSYHRALLLSKDKDTDGETEGGDTPALRDCPSPSLQKTPTHTPCSPGPLAQPTNRPPKEEDGDGQPSKPAGPKSAPLTPTKLHPTPLPSSPVHYPGPSLLHSPKPQPQGSPYRSQRALFSAQPQNQPQPQAQTGPAPFPQYNTQSAPPPPPPPPPAPPASTAYFPSQSPSPAGPFPGFKPAVTPPYPPCSQPLMQTLPHSVHYQSSAAPPPPPPPPPHPMPGPTLLHVNLQPPPIQQHQLMLTTAPPPPPPPQGQTSQQQQQQPPAGSSLLSLTPPPPPPPPPPAPSTNAPMQAHHFQNLGGFQPALLHPGGAATNPSVPQSTSPPPLQQTGLPPPPPPPPQQTQQGQAQAAASQMPSGTRGAPASSTPFHSSGYLSTGWH

Target ORF information:

RefSeq Version	XM_010738912.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010738912.2

ORF Insert Sequence:

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACAGCAGACA CCTCATACCT GGACATGGCT 
GCGGGCTCAG ACAGACCAGA ATCGGTGGAG GCCAGCCCTG TGGTGGTGGA AAAGTCCAGC 
TACCCACACC AGATCTACAG CAGCAGTTCT CACCATTCCC ACAGTTACAT TGGCCTGCCT 
TACGCTGACC ATAACTATGG GGCGCGGCCC CCACCTACAC CACCAGCCTC CCCTCCCCCC 
TCCATGTTGA TCCGACAAGG AGAGGGGGGG TTGTTTGTTC CAGGGGGCCA GGACGAAGCA 
TCAAGGGGCA CGACGCTCAG CACCTCAGAG GATGGCAGCT ACGGGGCTGA CATCACCCGC 
TGCATCTGTG GCTTCACCCA CGATGACGGC TACATGATCT GCTGTGATAA GTGCAGTGCC 
TGGCAGCATA TAGACTGCAT GGGCATCGAC AGGCAGAACA TTCCTGAGAC CTACCTGTGT 
GAACGCTGCC AACCACGACA CCTGGATCGA GAGAGGGCCA TCCTGCTGCA GACCAGGAAA 
CGGGAATGTT TGTCTGATGG GGACACGAGT GCTACAGAGA GTGGAGATGA GGTGCCGCTA 
GAGCTCTACT CCACCTTCCA ACACACGCCT ACCACCATCA CCCTGACTAC CGCGCGCCTT 
GGCAATAAGC AGGCTGATAA GAAACGCAAA AAGAGCGGTG ATAAAGAGCC TCCAGCGTCT 
TCTGCCCGAG CAAAGAAGGC CTTCCGAGAA GGGTCCAGAA AGTCCTCCAG AGTAAAGGGT 
GCAGCGCCAG AGCAGGAGCC AACAGAGTCC CTGTGGGAGA ACAAGATCAA GACGTGGATG 
GAGCGTTATG AGGAGGCCAG CAGCAATCAG TACAGCGAGG ATGTCCAGGT CCTGTTGCGT 
GTCAAAGAAC AAGGCGATGG CAAGAGCCTG GCGTACAACA CGCACCCTGC CTCTTTCAAA 
CCCCCTGTGG AGAGTCAAGT TCAGAAGAAC AAGAAGATCC TCAAAGCGGT GCGAGACTTG 
GCCCCAGACT CCCTCATCAT TGAGTACAGG GGCAAGTTCA TGCTCAGACA GCAGTTTGAG 
GCCAACGGAT ACTTCTTTAA GAGGCCATAC CCATTTGTGT TATTTTATTC TAAATTTGAC 
GGACTGGAGA TGTGCGTGGA CGCTCGTAGC TTCGGCAACG AAGCGCGCTT CATCCGTCGC 
TCCTGCACCC CTAATTCTGA AGTGCGGCAC GTCATTGAGG ACGGCATGCT GCATTTATAC 
ATCTACTCAT TGAGGCCGAT CACCAAGGGC ACAGAGATCA CCATCGGTTT TGATTTTGAC 
TATGGCAGCT GTAAATACAA GGTGGACTGT GCCTGCGTGA AGGGGAATCA GGAGTGCCCG 
GTGCTCAAGC ACAACCTCGA ACCCACAGAG AACCTGGTCG GCCGGCGCAG AGGGAGTCGT 
AAGGACAAGG AGACGGTTCG AGACGACCTG GGCCAGAACC AGAACATGGG TCTGGACGGC 
GAGGGGAAGG GCAAGAGCGT GGGCGACGGC AAACAGAGGA AGCTCTCTCC TCTTCGCCTC 
TCTATCTCAA ACAACCAGGA TCCTGAGTTA TATGAGGATC TAGAAGACAA AACCTCCGTT 
AGCAATGAAG TAGAGATGGA GTCAGAGGAG CAGATTGCAG AGAGGAGGAG GAAGATGACA 
CGAGAGGAGA GGAAGATGGA GGCCATCCTG CAAGCGTTTG CCCGAATGGA GAAGAGAGAG 
AAGCGAAGGG AGCAGGCGCT GGAAAGAATC GGTAGCATCA AGTCGGAGGT CGGGGGACGC 
AGTGACATCA AGGAGGAGCC TCCAGCCACG CCAGAGACGG CAGATTCTCC AGCTGTTATG 
CAGCCGCTCC TGGAGGTGAA AGAGGAGCCA GGATTAAAGC CGGCCAAGGT CAAAGGCTCG 
AGGAACAGGA AAAGTTTCTC AAGGAACCGC ACGCACATTG GCCAACAGCG CCGGAGAGCT 
CGCACCATCA GCACCTGCTC TGACCTGCAG CCTGGCTCTC CAATTGAATC TGTGGAGCCT 
CTGAGCAACG AAGCCCCCGA AGGAGAGACA CCCACCGCAC CAGAGCCGGA GGCAATCTCT 
TCCCAAGCAC CAGACACCAG CCCTCCGCAC AGCAGCTCAC CTGCACCTGA CAGAAACCGC 
ACCGGGAGCA AGAACTTCAA AACTAAAAAG CACTTTGTGA GTGAGTGGGT GGGAGAGAAG 
CAGCAGGACC GTGGTGCAGT ACGAACCCCA GAGCCGGCCC CCGAGAGGCC GCTGAGAATA 
AGCAGTGACC CCGAAGTACT CGCCACACAG CTGAACGCCC TCCCAGGCAT GGCCTGCTCT 
CCACAGGTCT ACAGCACGCC CAAACACTAC GTCCGCTTCT CGTCCCCTTT CCTGGCGAAC 
CGCAGTCCCA CAACTCCCGG AGTCCCCACT GGGAGACGGC GTTCCAGAGA ACTGCCAGAA 
ACGCCGCCAA CCACCGGCTC CTGCAAGAAG CGATGGTTGA AGCAGGCCCT TGAGGAAGAG 
GGATCCACCA GCCCAGCCAG ACGACCGAGC CTCCTCATGC CCTGTGAGGG TCCTCTTAGC 
CCCTCCATCA ACGGAGACTC TGACAGCCCT CTTCCCTACA ATGGCACCTG CTCGCTACCT 
GAGTTGCCCA CGCCTTTGAA AAAGCGCCGG TTGAGTCCAT TAGATGCCTG TATGTCTGAG 
AGCTCCACAC CCTACGGCTC CCCTTGTGCC ACGCCCACCA GGGCAGACCA ATCGGAGACA 
CCCGCAACCC CCATCTTACT GGCTACCCCA CCGCGTCCCA GAACGGAGGA GCCCAGTACA 
GAGCCCCCGC CCAACACCCC AACGCAGACA CTTAACGCCC CTCTGGAGAG TGAATCCTCT 
GTGGAGAGCT CCCCAGAGCT CAGCCGGAAA CCCAGTGTAC AAGAGGCCGA TCGTCCTCCT 
TCGTTGGTCT CCTCTCCCTG CATCAGGGCT CCCAGTTCAG ACGGACTCCC GGCTGAAGCC 
AAAGTGTCGG TTCCTGAGAG TCCACAGCCT CCGGTCGCCG ACTCTGTGGA CTGTGGAGAG 
GACAGGACCG ATGGTGGGGT TGTAGAAGGC AGTAGTGAGC CTTCCTCGTC CGCAGAAACA 
TGTGCTTCCT CTTTTCCTGG CTGGATAAAA AGCCCAGACA GAGGCCCGAC TGGACCAGCT 
GGCCTGAACT TCTCTCCAGT CAACTCAAAC CTAAGGGACC TCACCCCCTC ACACACTCTG 
GAGCCCCTGG CGGCTCCCTT CAGGCCCGAC GCTGCAGCTG GGGCTGCGGC AGGGTCCACA 
GCAGGGACCG GGTCATTGGT TGGCCCTCAA GCCCCCTTCA GTGAAGGCCA GGGGCAGCTG 
TTTTACCCCT GCTCTGAGGA GGGAGGTTCA CTCGGCTTCT CGCGCTCACT GAATGGGGAT 
GGCAGCGGTG AAGGAGGAGG ATCAGCGCAG AACCCCCCAC AGAAGAAAAA GGTTTCCCTG 
CTAGAATACA GGAAACGTCA GCGCGAAGCC CGTCGCAGCG GCTCCAAGAC CGAGTGCAGC 
TCTCCCGTCT CCACCGTGCC ACCTCTGACC GTGGACGCCT TCCCCGTCGC GCTGGAGACG 
ACAAGCGAAC CTCCACTACC CCCTGCTCCT ACGGCTGTCT GCAACGCCAA CAACACCATC 
GTTGTTAAAG AGCCCCAGAC GAGCGAGGAG GCCGAGACGC CCGGAGAGAA AGGCGAGAAG 
GAAGGAGGAG AAGGACAGTG GACGTCGTCC ACTTCTGTAG AGCAGGCCCG AGAACGCAGC 
TACCACAGAG CTCTGCTGCT CAGCAAAGAC AAGGACACGG ATGGTGAGAC AGAAGGTGGA 
GATACACCTG CACTGAGAGA TTGCCCGTCT CCAAGTCTTC AGAAGACCCC GACTCACACC 
CCCTGCTCTC CTGGTCCTCT CGCTCAGCCC ACCAATCGGC CACCGAAGGA GGAAGACGGC 
GACGGGCAGC CGAGCAAGCC TGCGGGACCC AAGTCGGCTC CTCTGACCCC CACAAAGCTG 
CATCCCACCC CGTTACCCTC CTCGCCGGTC CACTATCCGG GACCCTCCCT CCTGCACTCG 
CCCAAGCCTC AGCCTCAGGG CTCGCCGTAC CGCAGCCAGA GGGCGCTGTT CTCCGCTCAG 
CCTCAAAACC AACCGCAGCC CCAGGCTCAG ACTGGCCCAG CTCCTTTCCC TCAGTATAAC 
ACACAGAGTG CTCCACCGCC ACCGCCCCCT CCTCCACCAG CACCACCGGC CTCCACGGCG 
TATTTTCCCA GCCAGAGCCC CTCGCCGGCT GGACCTTTCC CCGGGTTCAA ACCCGCCGTC 
ACGCCCCCTT ACCCTCCGTG TTCTCAGCCC CTGATGCAGA CTCTTCCCCA CAGCGTGCAC 
TATCAGAGCT CAGCCGCTCC ACCGCCGCCT CCTCCTCCGC CCCCGCACCC GATGCCCGGC 
CCCACCCTGC TGCACGTCAA CCTGCAGCCC CCTCCCATCC AGCAGCACCA GCTCATGCTG 
ACCACCGCCC CTCCGCCGCC TCCTCCCCCA CAGGGCCAGA CCTCACAGCA GCAGCAGCAG 
CAGCCTCCCG CTGGCAGCTC CCTGCTGTCG CTCACCCCTC CTCCGCCGCC TCCTCCACCT 
CCTCCAGCCC CCTCGACCAA CGCCCCGATG CAGGCCCACC ACTTTCAGAA CTTAGGGGGT 
TTTCAGCCGG CGTTGCTACA CCCGGGCGGC GCCGCCACAA ACCCTTCAGT GCCCCAATCC 
ACGTCCCCCC CACCCCTTCA GCAGACCGGA CTGCCTCCAC CTCCCCCTCC TCCTCCCCAA 
CAGACTCAAC AAGGCCAGGC TCAGGCCGCC GCCTCCCAGA TGCCTTCTGG GACTCGTGGA 
GCTCCTGCGT CCTCGACCCC CTTCCACAGC TCGGGGTACC TGAGCACAGG GTGGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kmt2e ( XM_019278450.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019133995.1 Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OLa112878
Clone ID Related Accession (Same CDS sequence)	XM_019278450.1
Accession Version	XM_019278450.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	histone-lysine N-methyltransferase 2E isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017609671.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACAGCAGACA CCTCATACCT GGACATGGCT 
GCGGGCTCAG AACCAGAATC GGTGGAGGCC AGCCCTGTGG TGGTGGAAAA GTCCAGCTAC 
CCACACCAGA TCTACAGCAG CAGTTCTCAC CATTCCCACA GTTACATTGG CCTGCCTTAC 
GCTGACCATA ACTATGGGGC GCGGCCCCCA CCTACACCAC CAGCCTCCCC TCCCCCCTCC 
ATGTTGATCC GACAAGGAGA GGGGGGGTTG TTTGTTCCAG GGGGCCAGGA CGAAGCATCA 
AGGGGCACGA CGCTCAGCAC CTCAGAGGAT GGCAGCTACG GGGCTGACAT CACCCGCTGC 
ATCTGTGGCT TCACCCACGA TGACGGCTAC ATGATCTGCT GTGATAAGTG CAGTGCCTGG 
CAGCATATAG ACTGCATGGG CATCGACAGG CAGAACATTC CTGAGACCTA CCTGTGTGAA 
CGCTGCCAAC CACGACACCT GGATCGAGAG AGGGCCATCC TGCTGCAGAC CAGGAAACGG 
GAATGTTTGT CTGATGGGGA CACGAGTGCT ACAGAGAGTG GAGATGAGGT GCCGCTAGAG 
CTCTACTCCA CCTTCCAACA CACGCCTACC ACCATCACCC TGACTACCGC GCGCCTTGGC 
AATAAGCAGG CTGATAAGAA ACGCAAAAAG AGCGGTGATA AAGAGCCTCC AGCGTCTTCT 
GCCCGAGCAA AGAAGGCCTT CCGAGAAGGG TCCAGAAAGT CCTCCAGAGT AAAGGGTGCA 
GCGCCAGAGC AGGAGCCAAC AGAGTCCCTG TGGGAGAACA AGATCAAGAC GTGGATGGAG 
CGTTATGAGG AGGCCAGCAG CAATCAGTAC AGCGAGGATG TCCAGGTCCT GTTGCGTGTC 
AAAGAACAAG GCGATGGCAA GAGCCTGGCG TACAACACGC ACCCTGCCTC TTTCAAACCC 
CCTGTGGAGA GTCAAGTTCA GAAGAACAAG AAGATCCTCA AAGCGGTGCG AGACTTGGCC 
CCAGACTCCC TCATCATTGA GTACAGGGGC AAGTTCATGC TCAGACAGCA GTTTGAGGCC 
AACGGATACT TCTTTAAGAG GCCATACCCA TTTGTGTTAT TTTATTCTAA ATTTGACGGA 
CTGGAGATGT GCGTGGACGC TCGTAGCTTC GGCAACGAAG CGCGCTTCAT CCGTCGCTCC 
TGCACCCCTA ATTCTGAAGT GCGGCACGTC ATTGAGGACG GCATGCTGCA TTTATACATC 
TACTCATTGA GGCCGATCAC CAAGGGCACA GAGATCACCA TCGGTTTTGA TTTTGACTAT 
GGCAGCTGTA AATACAAGGT GGACTGTGCC TGCGTGAAGG GGAATCAGGA GTGCCCGGTG 
CTCAAGCACA ACCTCGAACC CACAGAGAAC CTGGTCGGCC GGCGCAGAGG GAGTCGTAAG 
GACAAGGAGA CGGTTCGAGA CGACCTGGGC CAGAACCAGA ACATGGGTCT GGACGGCGAG 
GGGAAGGGCA AGAGCGTGGG CGACGGCAAA CAGAGGAAGC TCTCTCCTCT TCGCCTCTCT 
ATCTCAAACA ACCAGGATCC TGAGTTATAT GAGGATCTAG AAGACAAAAC CTCCGTTAGC 
AATGAAGTAG AGATGGAGTC AGAGGAGCAG ATTGCAGAGA GGAGGAGGAA GATGACACGA 
GAGGAGAGGA AGATGGAGGC CATCCTGCAA GCGTTTGCCC GAATGGAGAA GAGAGAGAAG 
CGAAGGGAGC AGGCGCTGGA AAGAATCGGT AGCATCAAGT CGGAGGTCGG GGGACGCAGT 
GACATCAAGG AGGAGCCTCC AGCCACGCCA GAGACGGCAG ATTCTCCAGC TGTTATGCAG 
CCGCTCCTGG AGGTGAAAGA GGAGCCAGGA TTAAAGCCGG CCAAGGTCAA AGGCTCGAGG 
AACAGGAAAA GTTTCTCAAG GAACCGCACG CACATTGGCC AACAGCGCCG GAGAGCTCGC 
ACCATCAGCA CCTGCTCTGA CCTGCAGCCT GGCTCTCCAA TTGAATCTGT GGAGCCTCTG 
AGCAACGAAG CCCCCGAAGG AGAGACACCC ACCGCACCAG AGCCGGAGGC AATCTCTTCC 
CAAGCACCAG ACACCAGCCC TCCGCACAGC AGCTCACCTG CACCTGACAG AAACCGCACC 
GGGAGCAAGA ACTTCAAAAC TAAAAAGCAC TTTGTGAGTG AGTGGGTGGG AGAGAAGCAG 
CAGGACCGTG GTGCAGTACG AACCCCAGAG CCGGCCCCCG AGAGGCCGCT GAGAATAAGC 
AGTGACCCCG AAGTACTCGC CACACAGCTG AACGCCCTCC CAGGCATGGC CTGCTCTCCA 
CAGGTCTACA GCACGCCCAA ACACTACGTC CGCTTCTCGT CCCCTTTCCT GGCGAACCGC 
AGTCCCACAA CTCCCGGAGT CCCCACTGGG AGACGGCGTT CCAGAGAACT GCCAGAAACG 
CCGCCAACCA CCGGCTCCTG CAAGAAGCGA TGGTTGAAGC AGGCCCTTGA GGAAGAGGGA 
TCCACCAGCC CAGCCAGACG ACCGAGCCTC CTCATGCCCT GTGAGGGTCC TCTTAGCCCC 
TCCATCAACG GAGACTCTGA CAGCCCTCTT CCCTACAATG GCACCTGCTC GCTACCTGAG 
TTGCCCACGC CTTTGAAAAA GCGCCGGTTG AGTCCATTAG ATGCCTGTAT GTCTGAGAGC 
TCCACACCCT ACGGCTCCCC TTGTGCCACG CCCACCAGGG CAGACCAATC GGAGACACCC 
GCAACCCCCA TCTTACTGGC TACCCCACCG CGTCCCAGAA CGGAGGAGCC CAGTACAGAG 
CCCCCGCCCA ACACCCCAAC GCAGACACTT AACGCCCCTC TGGAGAGTGA ATCCTCTGTG 
GAGAGCTCCC CAGAGCTCAG CCGGAAACCC AGTGTACAAG AGGCCGATCG TCCTCCTTCG 
TTGGTCTCCT CTCCCTGCAT CAGGGCTCCC AGTTCAGACG GACTCCCGGC TGAAGCCAAA 
GTGTCGGTTC CTGAGAGTCC ACAGCCTCCG GTCGCCGACT CTGTGGACTG TGGAGAGGAC 
AGGACCGATG GTGGGGTTGT AGAAGGCAGT AGTGAGCCTT CCTCGTCCGC AGAAACATGT 
GCTTCCTCTT TTCCTGGCTG GATAAAAAGC CCAGACAGAG GCCCGACTGG ACCAGCTGGC 
CTGAACTTCT CTCCAGTCAA CTCAAACCTA AGGGACCTCA CCCCCTCACA CACTCTGGAG 
CCCCTGGCGG CTCCCTTCAG GCCCGACGCT GCAGCTGGGG CTGCGGCAGG GTCCACAGCA 
GGGACCGGGT CATTGGTTGG CCCTCAAGCC CCCTTCAGTG AAGGCCAGGG GCAGCTGTTT 
TACCCCTGCT CTGAGGAGGG AGGTTCACTC GGCTTCTCGC GCTCACTGAA TGGGGATGGC 
AGCGGTGAAG GAGGAGGATC AGCGCAGAAC CCCCCACAGA AGAAAAAGGT TTCCCTGCTA 
GAATACAGGA AACGTCAGCG CGAAGCCCGT CGCAGCGGCT CCAAGACCGA GTGCAGCTCT 
CCCGTCTCCA CCGTGCCACC TCTGACCGTG GACGCCTTCC CCGTCGCGCT GGAGACGACA 
AGCGAACCTC CACTACCCCC TGCTCCTACG GCTGTCTGCA ACGCCAACAA CACCATCGTT 
GTTAAAGAGC CCCAGACGAG CGAGGAGGCC GAGACGCCCG GAGAGAAAGG CGAGAAGGAA 
GGAGGAGAAG GACAGTGGAC GTCGTCCACT TCTGTAGAGC AGGCCCGAGA ACGCAGCTAC 
CACAGAGCTC TGCTGCTCAG CAAAGACAAG GACACGGATG GTGAGACAGA AGGTGGAGAT 
ACACCTGCAC TGAGAGATTG CCCGTCTCCA AGTCTTCAGA AGACCCCGAC TCACACCCCC 
TGCTCTCCTG GTCCTCTCGC TCAGCCCACC AATCGGCCAC CGAAGGAGGA AGACGGCGAC 
GGGCAGCCGA GCAAGCCTGC GGGACCCAAG TCGGCTCCTC TGACCCCCAC AAAGCTGCAT 
CCCACCCCGT TACCCTCCTC GCCGGTCCAC TATCCGGGAC CCTCCCTCCT GCACTCGCCC 
AAGCCTCAGC CTCAGGGCTC GCCGTACCGC AGCCAGAGGG CGCTGTTCTC CGCTCAGCCT 
CAAAACCAAC CGCAGCCCCA GGCTCAGACT GGCCCAGCTC CTTTCCCTCA GTATAACACA 
CAGAGTGCTC CACCGCCACC GCCCCCTCCT CCACCAGCAC CACCGGCCTC CACGGCGTAT 
TTTCCCAGCC AGAGCCCCTC GCCGGCTGGA CCTTTCCCCG GGTTCAAACC CGCCGTCACG 
CCCCCTTACC CTCCGTGTTC TCAGCCCCTG ATGCAGACTC TTCCCCACAG CGTGCACTAT 
CAGAGCTCAG CCGCTCCACC GCCGCCTCCT CCTCCGCCCC CGCACCCGAT GCCCGGCCCC 
ACCCTGCTGC ACGTCAACCT GCAGCCCCCT CCCATCCAGC AGCACCAGCT CATGCTGACC 
ACCGCCCCTC CGCCGCCTCC TCCCCCACAG GGCCAGACCT CACAGCAGCA GCAGCAGCAG 
CCTCCCGCTG GCAGCTCCCT GCTGTCGCTC ACCCCTCCTC CGCCGCCTCC TCCACCTCCT 
CCAGCCCCCT CGACCAACGC CCCGATGCAG GCCCACCACT TTCAGAACTT AGGGGGTTTT 
CAGCCGGCGT TGCTACACCC GGGCGGCGCC GCCACAAACC CTTCAGTGCC CCAATCCACG 
TCCCCCCCAC CCCTTCAGCA GACCGGACTG CCTCCACCTC CCCCTCCTCC TCCCCAACAG 
ACTCAACAAG GCCAGGCTCA GGCCGCCGCC TCCCAGATGC CTTCTGGGAC TCGTGGAGCT 
CCTGCGTCCT CGACCCCCTT CCACAGCTCG GGGTACCTGA GCACAGGGTG GCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019133995.1
CDS	492..5408
Translation	MSIVIPVGVDTADTSYLDMAAGSEPESVEASPVVVEKSSYPHQIYSSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPSMLIRQGEGGLFVPGGQDEASRGTTLSTSEDGSYGADITRCICGFTHDDGYMICCDKCSAWQHIDCMGIDRQNIPETYLCERCQPRHLDRERAILLQTRKRECLSDGDTSATESGDEVPLELYSTFQHTPTTITLTTARLGNKQADKKRKKSGDKEPPASSARAKKAFREGSRKSSRVKGAAPEQEPTESLWENKIKTWMERYEEASSNQYSEDVQVLLRVKEQGDGKSLAYNTHPASFKPPVESQVQKNKKILKAVRDLAPDSLIIEYRGKFMLRQQFEANGYFFKRPYPFVLFYSKFDGLEMCVDARSFGNEARFIRRSCTPNSEVRHVIEDGMLHLYIYSLRPITKGTEITIGFDFDYGSCKYKVDCACVKGNQECPVLKHNLEPTENLVGRRRGSRKDKETVRDDLGQNQNMGLDGEGKGKSVGDGKQRKLSPLRLSISNNQDPELYEDLEDKTSVSNEVEMESEEQIAERRRKMTREERKMEAILQAFARMEKREKRREQALERIGSIKSEVGGRSDIKEEPPATPETADSPAVMQPLLEVKEEPGLKPAKVKGSRNRKSFSRNRTHIGQQRRRARTISTCSDLQPGSPIESVEPLSNEAPEGETPTAPEPEAISSQAPDTSPPHSSSPAPDRNRTGSKNFKTKKHFVSEWVGEKQQDRGAVRTPEPAPERPLRISSDPEVLATQLNALPGMACSPQVYSTPKHYVRFSSPFLANRSPTTPGVPTGRRRSRELPETPPTTGSCKKRWLKQALEEEGSTSPARRPSLLMPCEGPLSPSINGDSDSPLPYNGTCSLPELPTPLKKRRLSPLDACMSESSTPYGSPCATPTRADQSETPATPILLATPPRPRTEEPSTEPPPNTPTQTLNAPLESESSVESSPELSRKPSVQEADRPPSLVSSPCIRAPSSDGLPAEAKVSVPESPQPPVADSVDCGEDRTDGGVVEGSSEPSSSAETCASSFPGWIKSPDRGPTGPAGLNFSPVNSNLRDLTPSHTLEPLAAPFRPDAAAGAAAGSTAGTGSLVGPQAPFSEGQGQLFYPCSEEGGSLGFSRSLNGDGSGEGGGSAQNPPQKKKVSLLEYRKRQREARRSGSKTECSSPVSTVPPLTVDAFPVALETTSEPPLPPAPTAVCNANNTIVVKEPQTSEEAETPGEKGEKEGGEGQWTSSTSVEQARERSYHRALLLSKDKDTDGETEGGDTPALRDCPSPSLQKTPTHTPCSPGPLAQPTNRPPKEEDGDGQPSKPAGPKSAPLTPTKLHPTPLPSSPVHYPGPSLLHSPKPQPQGSPYRSQRALFSAQPQNQPQPQAQTGPAPFPQYNTQSAPPPPPPPPPAPPASTAYFPSQSPSPAGPFPGFKPAVTPPYPPCSQPLMQTLPHSVHYQSSAAPPPPPPPPPHPMPGPTLLHVNLQPPPIQQHQLMLTTAPPPPPPPQGQTSQQQQQQPPAGSSLLSLTPPPPPPPPPPAPSTNAPMQAHHFQNLGGFQPALLHPGGAATNPSVPQSTSPPPLQQTGLPPPPPPPPQQTQQGQAQAAASQMPSGTRGAPASSTPFHSSGYLSTGWH

Target ORF information:

RefSeq Version	XM_019278450.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019278450.1

ORF Insert Sequence:

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACAGCAGACA CCTCATACCT GGACATGGCT 
GCGGGCTCAG AACCAGAATC GGTGGAGGCC AGCCCTGTGG TGGTGGAAAA GTCCAGCTAC 
CCACACCAGA TCTACAGCAG CAGTTCTCAC CATTCCCACA GTTACATTGG CCTGCCTTAC 
GCTGACCATA ACTATGGGGC GCGGCCCCCA CCTACACCAC CAGCCTCCCC TCCCCCCTCC 
ATGTTGATCC GACAAGGAGA GGGGGGGTTG TTTGTTCCAG GGGGCCAGGA CGAAGCATCA 
AGGGGCACGA CGCTCAGCAC CTCAGAGGAT GGCAGCTACG GGGCTGACAT CACCCGCTGC 
ATCTGTGGCT TCACCCACGA TGACGGCTAC ATGATCTGCT GTGATAAGTG CAGTGCCTGG 
CAGCATATAG ACTGCATGGG CATCGACAGG CAGAACATTC CTGAGACCTA CCTGTGTGAA 
CGCTGCCAAC CACGACACCT GGATCGAGAG AGGGCCATCC TGCTGCAGAC CAGGAAACGG 
GAATGTTTGT CTGATGGGGA CACGAGTGCT ACAGAGAGTG GAGATGAGGT GCCGCTAGAG 
CTCTACTCCA CCTTCCAACA CACGCCTACC ACCATCACCC TGACTACCGC GCGCCTTGGC 
AATAAGCAGG CTGATAAGAA ACGCAAAAAG AGCGGTGATA AAGAGCCTCC AGCGTCTTCT 
GCCCGAGCAA AGAAGGCCTT CCGAGAAGGG TCCAGAAAGT CCTCCAGAGT AAAGGGTGCA 
GCGCCAGAGC AGGAGCCAAC AGAGTCCCTG TGGGAGAACA AGATCAAGAC GTGGATGGAG 
CGTTATGAGG AGGCCAGCAG CAATCAGTAC AGCGAGGATG TCCAGGTCCT GTTGCGTGTC 
AAAGAACAAG GCGATGGCAA GAGCCTGGCG TACAACACGC ACCCTGCCTC TTTCAAACCC 
CCTGTGGAGA GTCAAGTTCA GAAGAACAAG AAGATCCTCA AAGCGGTGCG AGACTTGGCC 
CCAGACTCCC TCATCATTGA GTACAGGGGC AAGTTCATGC TCAGACAGCA GTTTGAGGCC 
AACGGATACT TCTTTAAGAG GCCATACCCA TTTGTGTTAT TTTATTCTAA ATTTGACGGA 
CTGGAGATGT GCGTGGACGC TCGTAGCTTC GGCAACGAAG CGCGCTTCAT CCGTCGCTCC 
TGCACCCCTA ATTCTGAAGT GCGGCACGTC ATTGAGGACG GCATGCTGCA TTTATACATC 
TACTCATTGA GGCCGATCAC CAAGGGCACA GAGATCACCA TCGGTTTTGA TTTTGACTAT 
GGCAGCTGTA AATACAAGGT GGACTGTGCC TGCGTGAAGG GGAATCAGGA GTGCCCGGTG 
CTCAAGCACA ACCTCGAACC CACAGAGAAC CTGGTCGGCC GGCGCAGAGG GAGTCGTAAG 
GACAAGGAGA CGGTTCGAGA CGACCTGGGC CAGAACCAGA ACATGGGTCT GGACGGCGAG 
GGGAAGGGCA AGAGCGTGGG CGACGGCAAA CAGAGGAAGC TCTCTCCTCT TCGCCTCTCT 
ATCTCAAACA ACCAGGATCC TGAGTTATAT GAGGATCTAG AAGACAAAAC CTCCGTTAGC 
AATGAAGTAG AGATGGAGTC AGAGGAGCAG ATTGCAGAGA GGAGGAGGAA GATGACACGA 
GAGGAGAGGA AGATGGAGGC CATCCTGCAA GCGTTTGCCC GAATGGAGAA GAGAGAGAAG 
CGAAGGGAGC AGGCGCTGGA AAGAATCGGT AGCATCAAGT CGGAGGTCGG GGGACGCAGT 
GACATCAAGG AGGAGCCTCC AGCCACGCCA GAGACGGCAG ATTCTCCAGC TGTTATGCAG 
CCGCTCCTGG AGGTGAAAGA GGAGCCAGGA TTAAAGCCGG CCAAGGTCAA AGGCTCGAGG 
AACAGGAAAA GTTTCTCAAG GAACCGCACG CACATTGGCC AACAGCGCCG GAGAGCTCGC 
ACCATCAGCA CCTGCTCTGA CCTGCAGCCT GGCTCTCCAA TTGAATCTGT GGAGCCTCTG 
AGCAACGAAG CCCCCGAAGG AGAGACACCC ACCGCACCAG AGCCGGAGGC AATCTCTTCC 
CAAGCACCAG ACACCAGCCC TCCGCACAGC AGCTCACCTG CACCTGACAG AAACCGCACC 
GGGAGCAAGA ACTTCAAAAC TAAAAAGCAC TTTGTGAGTG AGTGGGTGGG AGAGAAGCAG 
CAGGACCGTG GTGCAGTACG AACCCCAGAG CCGGCCCCCG AGAGGCCGCT GAGAATAAGC 
AGTGACCCCG AAGTACTCGC CACACAGCTG AACGCCCTCC CAGGCATGGC CTGCTCTCCA 
CAGGTCTACA GCACGCCCAA ACACTACGTC CGCTTCTCGT CCCCTTTCCT GGCGAACCGC 
AGTCCCACAA CTCCCGGAGT CCCCACTGGG AGACGGCGTT CCAGAGAACT GCCAGAAACG 
CCGCCAACCA CCGGCTCCTG CAAGAAGCGA TGGTTGAAGC AGGCCCTTGA GGAAGAGGGA 
TCCACCAGCC CAGCCAGACG ACCGAGCCTC CTCATGCCCT GTGAGGGTCC TCTTAGCCCC 
TCCATCAACG GAGACTCTGA CAGCCCTCTT CCCTACAATG GCACCTGCTC GCTACCTGAG 
TTGCCCACGC CTTTGAAAAA GCGCCGGTTG AGTCCATTAG ATGCCTGTAT GTCTGAGAGC 
TCCACACCCT ACGGCTCCCC TTGTGCCACG CCCACCAGGG CAGACCAATC GGAGACACCC 
GCAACCCCCA TCTTACTGGC TACCCCACCG CGTCCCAGAA CGGAGGAGCC CAGTACAGAG 
CCCCCGCCCA ACACCCCAAC GCAGACACTT AACGCCCCTC TGGAGAGTGA ATCCTCTGTG 
GAGAGCTCCC CAGAGCTCAG CCGGAAACCC AGTGTACAAG AGGCCGATCG TCCTCCTTCG 
TTGGTCTCCT CTCCCTGCAT CAGGGCTCCC AGTTCAGACG GACTCCCGGC TGAAGCCAAA 
GTGTCGGTTC CTGAGAGTCC ACAGCCTCCG GTCGCCGACT CTGTGGACTG TGGAGAGGAC 
AGGACCGATG GTGGGGTTGT AGAAGGCAGT AGTGAGCCTT CCTCGTCCGC AGAAACATGT 
GCTTCCTCTT TTCCTGGCTG GATAAAAAGC CCAGACAGAG GCCCGACTGG ACCAGCTGGC 
CTGAACTTCT CTCCAGTCAA CTCAAACCTA AGGGACCTCA CCCCCTCACA CACTCTGGAG 
CCCCTGGCGG CTCCCTTCAG GCCCGACGCT GCAGCTGGGG CTGCGGCAGG GTCCACAGCA 
GGGACCGGGT CATTGGTTGG CCCTCAAGCC CCCTTCAGTG AAGGCCAGGG GCAGCTGTTT 
TACCCCTGCT CTGAGGAGGG AGGTTCACTC GGCTTCTCGC GCTCACTGAA TGGGGATGGC 
AGCGGTGAAG GAGGAGGATC AGCGCAGAAC CCCCCACAGA AGAAAAAGGT TTCCCTGCTA 
GAATACAGGA AACGTCAGCG CGAAGCCCGT CGCAGCGGCT CCAAGACCGA GTGCAGCTCT 
CCCGTCTCCA CCGTGCCACC TCTGACCGTG GACGCCTTCC CCGTCGCGCT GGAGACGACA 
AGCGAACCTC CACTACCCCC TGCTCCTACG GCTGTCTGCA ACGCCAACAA CACCATCGTT 
GTTAAAGAGC CCCAGACGAG CGAGGAGGCC GAGACGCCCG GAGAGAAAGG CGAGAAGGAA 
GGAGGAGAAG GACAGTGGAC GTCGTCCACT TCTGTAGAGC AGGCCCGAGA ACGCAGCTAC 
CACAGAGCTC TGCTGCTCAG CAAAGACAAG GACACGGATG GTGAGACAGA AGGTGGAGAT 
ACACCTGCAC TGAGAGATTG CCCGTCTCCA AGTCTTCAGA AGACCCCGAC TCACACCCCC 
TGCTCTCCTG GTCCTCTCGC TCAGCCCACC AATCGGCCAC CGAAGGAGGA AGACGGCGAC 
GGGCAGCCGA GCAAGCCTGC GGGACCCAAG TCGGCTCCTC TGACCCCCAC AAAGCTGCAT 
CCCACCCCGT TACCCTCCTC GCCGGTCCAC TATCCGGGAC CCTCCCTCCT GCACTCGCCC 
AAGCCTCAGC CTCAGGGCTC GCCGTACCGC AGCCAGAGGG CGCTGTTCTC CGCTCAGCCT 
CAAAACCAAC CGCAGCCCCA GGCTCAGACT GGCCCAGCTC CTTTCCCTCA GTATAACACA 
CAGAGTGCTC CACCGCCACC GCCCCCTCCT CCACCAGCAC CACCGGCCTC CACGGCGTAT 
TTTCCCAGCC AGAGCCCCTC GCCGGCTGGA CCTTTCCCCG GGTTCAAACC CGCCGTCACG 
CCCCCTTACC CTCCGTGTTC TCAGCCCCTG ATGCAGACTC TTCCCCACAG CGTGCACTAT 
CAGAGCTCAG CCGCTCCACC GCCGCCTCCT CCTCCGCCCC CGCACCCGAT GCCCGGCCCC 
ACCCTGCTGC ACGTCAACCT GCAGCCCCCT CCCATCCAGC AGCACCAGCT CATGCTGACC 
ACCGCCCCTC CGCCGCCTCC TCCCCCACAG GGCCAGACCT CACAGCAGCA GCAGCAGCAG 
CCTCCCGCTG GCAGCTCCCT GCTGTCGCTC ACCCCTCCTC CGCCGCCTCC TCCACCTCCT 
CCAGCCCCCT CGACCAACGC CCCGATGCAG GCCCACCACT TTCAGAACTT AGGGGGTTTT 
CAGCCGGCGT TGCTACACCC GGGCGGCGCC GCCACAAACC CTTCAGTGCC CCAATCCACG 
TCCCCCCCAC CCCTTCAGCA GACCGGACTG CCTCCACCTC CCCCTCCTCC TCCCCAACAG 
ACTCAACAAG GCCAGGCTCA GGCCGCCGCC TCCCAGATGC CTTCTGGGAC TCGTGGAGCT 
CCTGCGTCCT CGACCCCCTT CCACAGCTCG GGGTACCTGA GCACAGGGTG GCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kmt2e ( XM_019278451.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019133996.1 Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X7, mRNA.

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CloneID	OLa112879
Clone ID Related Accession (Same CDS sequence)	XM_019278451.1
Accession Version	XM_019278451.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	histone-lysine N-methyltransferase 2E isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017609671.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACAGCAGACA CCTCATACCT GGACATGGCT 
GCGGGCTCAG ACAGACCAGA ATCGGTGGAG GCCAGCCCTG TGGTGGTGGA AAAGTCCAGC 
TACCCACACC AGATCTACAG CAGCAGTTCT CACCATTCCC ACAGTTACAT TGGCCTGCCT 
TACGCTGACC ATAACTATGG GGCGCGGCCC CCACCTACAC CACCAGCCTC CCCTCCCCCC 
TCCATGTTGA TCCGACAAGG AGAGGGGGGG TTGTTTGTTC CAGGGGGCCA GGACGAAGCA 
TCAAGGGGCA CGACGCTCAG CACCTCAGAG GATGGCAGCT ACGGGGCTGA CATCACCCGC 
TGCATCTGTG GCTTCACCCA CGATGACGGC TACATGATCT GCTGTGATAA GTGCAGTGCC 
TGGCAGCATA TAGACTGCAT GGGCATCGAC AGGCAGAACA TTCCTGAGAC CTACCTGTGT 
GAACGCTGCC AACCACGACA CCTGGATCGA GAGAGGGCCA TCCTGCTGCA GACCAGGAAA 
CGGGAATGTT TGTCTGATGG GGACACGAGT GCTACAGAGA GTGGAGATGA GGTGCCGCTA 
GAGCTCTACT CCACCTTCCA ACACACGCCT ACCACCATCA CCCTGACTAC CGCGCGCCTT 
GGCAATAAGC AGGCTGATAA GAAACGCAAA AAGAGCGGTG ATAAAGAGCC TCCAGCGTCT 
TCTGCCCGAG CAAAGAAGGC CTTCCGAGAA GGGTCCAGAA AGTCCTCCAG AGTAAAGGGT 
GCAGCGCCAG AGCAGGAGCC AACAGAGTCC CTGTGGGAGA ACAAGATCAA GACGTGGATG 
GAGCGTTATG AGGAGGCCAG CAGCAATCAG TACAGCGAGG ATGTCCAGGT CCTGTTGCGT 
GTCAAAGAAC AAGGCGATGG CAAGAGCCTG GCGTACAACA CGCACCCTGC CTCTTTCAAA 
CCCCCTGTGG AGAGTCAAGT TCAGAAGAAC AAGAAGATCC TCAAAGCGGT GCGAGACTTG 
GCCCCAGACT CCCTCATCAT TGAGTACAGG GGCAAGTTCA TGCTCAGACA GCAGTTTGAG 
GCCAACGGAT ACTTCTTTAA GAGGCCATAC CCATTTGTGT TATTTTATTC TAAATTTGAC 
GGACTGGAGA TGTGCGTGGA CGCTCGTAGC TTCGGCAACG AAGCGCGCTT CATCCGTCGC 
TCCTGCACCC CTAATTCTGA AGTGCGGCAC GTCATTGAGG ACGGCATGCT GCATTTATAC 
ATCTACTCAT TGAGGCCGAT CACCAAGGGC ACAGAGATCA CCATCGGTTT TGATTTTGAC 
TATGGCAGCT GTAAATACAA GGTGGACTGT GCCTGCGTGA AGGGGAATCA GGAGTGCCCG 
GTGCTCAAGC ACAACCTCGA ACCCACAGAG AACCTGGTCG GCCGGCGCAG AGGGAGTCGT 
AAGGACAAGG AGACGGTTCG AGACGACCTG GGCCAGAACC AGAACATGGG TCTGGACGGC 
GAGGGGAAGG GCAAGAGCGT GGGCGACGGC AAACAGAGGA AGCTCTCTCC TCTTCGCCTC 
TCTATCTCAA ACAACCAGGC CAACCCAGCG GAGCAGTCCC ATCTCCCTGT CGGGGCGGCT 
TCCAGCTGGA AAGAACTGAA ACACAAGGAG ACACGAGAGG AGAGGAAGAT GGAGGCCATC 
CTGCAAGCGT TTGCCCGAAT GGAGAAGAGA GAGAAGCGAA GGGAGCAGGC GCTGGAAAGA 
ATCGGTAGCA TCAAGTCGGA GGTCGGGGGA CGCAGTGACA TCAAGGAGGA GCCTCCAGCC 
ACGCCAGAGA CGGCAGATTC TCCAGCTGTT ATGCAGCCGC TCCTGGAGGT GAAAGAGGAG 
CCAGGATTAA AGCCGGCCAA GGTCAAAGGC TCGAGGAACA GGAAAAGTTT CTCAAGGAAC 
CGCACGCACA TTGGCCAACA GCGCCGGAGA GCTCGCACCA TCAGCACCTG CTCTGACCTG 
CAGCCTGGCT CTCCAATTGA ATCTGTGGAG CCTCTGAGCA ACGAAGCCCC CGAAGGAGAG 
ACACCCACCG CACCAGAGCC GGAGGCAATC TCTTCCCAAG CACCAGACAC CAGCCCTCCG 
CACAGCAGCT CACCTGCACC TGACAGAAAC CGCACCGGGA GCAAGAACTT CAAAACTAAA 
AAGCACTTTG TGAGTGAGTG GGTGGGAGAG AAGCAGCAGG ACCGTGGTGC AGTACGAACC 
CCAGAGCCGG CCCCCGAGAG GCCGCTGAGA ATAAGCAGTG ACCCCGAAGT ACTCGCCACA 
CAGCTGAACG CCCTCCCAGG CATGGCCTGC TCTCCACAGG TCTACAGCAC GCCCAAACAC 
TACGTCCGCT TCTCGTCCCC TTTCCTGGCG AACCGCAGTC CCACAACTCC CGGAGTCCCC 
ACTGGGAGAC GGCGTTCCAG AGAACTGCCA GAAACGCCGC CAACCACCGG CTCCTGCAAG 
AAGCGATGGT TGAAGCAGGC CCTTGAGGAA GAGGGATCCA CCAGCCCAGC CAGACGACCG 
AGCCTCCTCA TGCCCTGTGA GGGTCCTCTT AGCCCCTCCA TCAACGGAGA CTCTGACAGC 
CCTCTTCCCT ACAATGGCAC CTGCTCGCTA CCTGAGTTGC CCACGCCTTT GAAAAAGCGC 
CGGTTGAGTC CATTAGATGC CTGTATGTCT GAGAGCTCCA CACCCTACGG CTCCCCTTGT 
GCCACGCCCA CCAGGGCAGA CCAATCGGAG ACACCCGCAA CCCCCATCTT ACTGGCTACC 
CCACCGCGTC CCAGAACGGA GGAGCCCAGT ACAGAGCCCC CGCCCAACAC CCCAACGCAG 
ACACTTAACG CCCCTCTGGA GAGTGAATCC TCTGTGGAGA GCTCCCCAGA GCTCAGCCGG 
AAACCCAGTG TACAAGAGGC CGATCGTCCT CCTTCGTTGG TCTCCTCTCC CTGCATCAGG 
GCTCCCAGTT CAGACGGACT CCCGGCTGAA GCCAAAGTGT CGGTTCCTGA GAGTCCACAG 
CCTCCGGTCG CCGACTCTGT GGACTGTGGA GAGGACAGGA CCGATGGTGG GGTTGTAGAA 
GGCAGTAGTG AGCCTTCCTC GTCCGCAGAA ACATGTGCTT CCTCTTTTCC TGGCTGGATA 
AAAAGCCCAG ACAGAGGCCC GACTGGACCA GCTGGCCTGA ACTTCTCTCC AGTCAACTCA 
AACCTAAGGG ACCTCACCCC CTCACACACT CTGGAGCCCC TGGCGGCTCC CTTCAGGCCC 
GACGCTGCAG CTGGGGCTGC GGCAGGGTCC ACAGCAGGGA CCGGGTCATT GGTTGGCCCT 
CAAGCCCCCT TCAGTGAAGG CCAGGGGCAG CTGTTTTACC CCTGCTCTGA GGAGGGAGGT 
TCACTCGGCT TCTCGCGCTC ACTGAATGGG GATGGCAGCG GTGAAGGAGG AGGATCAGCG 
CAGAACCCCC CACAGAAGAA AAAGGTTTCC CTGCTAGAAT ACAGGAAACG TCAGCGCGAA 
GCCCGTCGCA GCGGCTCCAA GACCGAGTGC AGCTCTCCCG TCTCCACCGT GCCACCTCTG 
ACCGTGGACG CCTTCCCCGT CGCGCTGGAG ACGACAAGCG AACCTCCACT ACCCCCTGCT 
CCTACGGCTG TCTGCAACGC CAACAACACC ATCGTTGTTA AAGAGCCCCA GACGAGCGAG 
GAGGCCGAGA CGCCCGGAGA GAAAGGCGAG AAGGAAGGAG GAGAAGGACA GTGGACGTCG 
TCCACTTCTG TAGAGCAGGC CCGAGAACGC AGCTACCACA GAGCTCTGCT GCTCAGCAAA 
GACAAGGACA CGGATGGTGA GACAGAAGGT GGAGATACAC CTGCACTGAG AGATTGCCCG 
TCTCCAAGTC TTCAGAAGAC CCCGACTCAC ACCCCCTGCT CTCCTGGTCC TCTCGCTCAG 
CCCACCAATC GGCCACCGAA GGAGGAAGAC GGCGACGGGC AGCCGAGCAA GCCTGCGGGA 
CCCAAGTCGG CTCCTCTGAC CCCCACAAAG CTGCATCCCA CCCCGTTACC CTCCTCGCCG 
GTCCACTATC CGGGACCCTC CCTCCTGCAC TCGCCCAAGC CTCAGCCTCA GGGCTCGCCG 
TACCGCAGCC AGAGGGCGCT GTTCTCCGCT CAGCCTCAAA ACCAACCGCA GCCCCAGGCT 
CAGACTGGCC CAGCTCCTTT CCCTCAGTAT AACACACAGA GTGCTCCACC GCCACCGCCC 
CCTCCTCCAC CAGCACCACC GGCCTCCACG GCGTATTTTC CCAGCCAGAG CCCCTCGCCG 
GCTGGACCTT TCCCCGGGTT CAAACCCGCC GTCACGCCCC CTTACCCTCC GTGTTCTCAG 
CCCCTGATGC AGACTCTTCC CCACAGCGTG CACTATCAGA GCTCAGCCGC TCCACCGCCG 
CCTCCTCCTC CGCCCCCGCA CCCGATGCCC GGCCCCACCC TGCTGCACGT CAACCTGCAG 
CCCCCTCCCA TCCAGCAGCA CCAGCTCATG CTGACCACCG CCCCTCCGCC GCCTCCTCCC 
CCACAGGGCC AGACCTCACA GCAGCAGCAG CAGCAGCCTC CCGCTGGCAG CTCCCTGCTG 
TCGCTCACCC CTCCTCCGCC GCCTCCTCCA CCTCCTCCAG CCCCCTCGAC CAACGCCCCG 
ATGCAGGCCC ACCACTTTCA GAACTTAGGG GGTTTTCAGC CGGCGTTGCT ACACCCGGGC 
GGCGCCGCCA CAAACCCTTC AGTGCCCCAA TCCACGTCCC CCCCACCCCT TCAGCAGACC 
GGACTGCCTC CACCTCCCCC TCCTCCTCCC CAACAGACTC AACAAGGCCA GGCTCAGGCC 
GCCGCCTCCC AGATGCCTTC TGGGACTCGT GGAGCTCCTG CGTCCTCGAC CCCCTTCCAC 
AGCTCGGGGT ACCTGAGCAC AGGGTGGCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019133996.1
CDS	492..5384
Translation	MSIVIPVGVDTADTSYLDMAAGSDRPESVEASPVVVEKSSYPHQIYSSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPSMLIRQGEGGLFVPGGQDEASRGTTLSTSEDGSYGADITRCICGFTHDDGYMICCDKCSAWQHIDCMGIDRQNIPETYLCERCQPRHLDRERAILLQTRKRECLSDGDTSATESGDEVPLELYSTFQHTPTTITLTTARLGNKQADKKRKKSGDKEPPASSARAKKAFREGSRKSSRVKGAAPEQEPTESLWENKIKTWMERYEEASSNQYSEDVQVLLRVKEQGDGKSLAYNTHPASFKPPVESQVQKNKKILKAVRDLAPDSLIIEYRGKFMLRQQFEANGYFFKRPYPFVLFYSKFDGLEMCVDARSFGNEARFIRRSCTPNSEVRHVIEDGMLHLYIYSLRPITKGTEITIGFDFDYGSCKYKVDCACVKGNQECPVLKHNLEPTENLVGRRRGSRKDKETVRDDLGQNQNMGLDGEGKGKSVGDGKQRKLSPLRLSISNNQANPAEQSHLPVGAASSWKELKHKETREERKMEAILQAFARMEKREKRREQALERIGSIKSEVGGRSDIKEEPPATPETADSPAVMQPLLEVKEEPGLKPAKVKGSRNRKSFSRNRTHIGQQRRRARTISTCSDLQPGSPIESVEPLSNEAPEGETPTAPEPEAISSQAPDTSPPHSSSPAPDRNRTGSKNFKTKKHFVSEWVGEKQQDRGAVRTPEPAPERPLRISSDPEVLATQLNALPGMACSPQVYSTPKHYVRFSSPFLANRSPTTPGVPTGRRRSRELPETPPTTGSCKKRWLKQALEEEGSTSPARRPSLLMPCEGPLSPSINGDSDSPLPYNGTCSLPELPTPLKKRRLSPLDACMSESSTPYGSPCATPTRADQSETPATPILLATPPRPRTEEPSTEPPPNTPTQTLNAPLESESSVESSPELSRKPSVQEADRPPSLVSSPCIRAPSSDGLPAEAKVSVPESPQPPVADSVDCGEDRTDGGVVEGSSEPSSSAETCASSFPGWIKSPDRGPTGPAGLNFSPVNSNLRDLTPSHTLEPLAAPFRPDAAAGAAAGSTAGTGSLVGPQAPFSEGQGQLFYPCSEEGGSLGFSRSLNGDGSGEGGGSAQNPPQKKKVSLLEYRKRQREARRSGSKTECSSPVSTVPPLTVDAFPVALETTSEPPLPPAPTAVCNANNTIVVKEPQTSEEAETPGEKGEKEGGEGQWTSSTSVEQARERSYHRALLLSKDKDTDGETEGGDTPALRDCPSPSLQKTPTHTPCSPGPLAQPTNRPPKEEDGDGQPSKPAGPKSAPLTPTKLHPTPLPSSPVHYPGPSLLHSPKPQPQGSPYRSQRALFSAQPQNQPQPQAQTGPAPFPQYNTQSAPPPPPPPPPAPPASTAYFPSQSPSPAGPFPGFKPAVTPPYPPCSQPLMQTLPHSVHYQSSAAPPPPPPPPPHPMPGPTLLHVNLQPPPIQQHQLMLTTAPPPPPPPQGQTSQQQQQQPPAGSSLLSLTPPPPPPPPPPAPSTNAPMQAHHFQNLGGFQPALLHPGGAATNPSVPQSTSPPPLQQTGLPPPPPPPPQQTQQGQAQAAASQMPSGTRGAPASSTPFHSSGYLSTGWH

Target ORF information:

RefSeq Version	XM_019278451.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019278451.1

ORF Insert Sequence:

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACAGCAGACA CCTCATACCT GGACATGGCT 
GCGGGCTCAG ACAGACCAGA ATCGGTGGAG GCCAGCCCTG TGGTGGTGGA AAAGTCCAGC 
TACCCACACC AGATCTACAG CAGCAGTTCT CACCATTCCC ACAGTTACAT TGGCCTGCCT 
TACGCTGACC ATAACTATGG GGCGCGGCCC CCACCTACAC CACCAGCCTC CCCTCCCCCC 
TCCATGTTGA TCCGACAAGG AGAGGGGGGG TTGTTTGTTC CAGGGGGCCA GGACGAAGCA 
TCAAGGGGCA CGACGCTCAG CACCTCAGAG GATGGCAGCT ACGGGGCTGA CATCACCCGC 
TGCATCTGTG GCTTCACCCA CGATGACGGC TACATGATCT GCTGTGATAA GTGCAGTGCC 
TGGCAGCATA TAGACTGCAT GGGCATCGAC AGGCAGAACA TTCCTGAGAC CTACCTGTGT 
GAACGCTGCC AACCACGACA CCTGGATCGA GAGAGGGCCA TCCTGCTGCA GACCAGGAAA 
CGGGAATGTT TGTCTGATGG GGACACGAGT GCTACAGAGA GTGGAGATGA GGTGCCGCTA 
GAGCTCTACT CCACCTTCCA ACACACGCCT ACCACCATCA CCCTGACTAC CGCGCGCCTT 
GGCAATAAGC AGGCTGATAA GAAACGCAAA AAGAGCGGTG ATAAAGAGCC TCCAGCGTCT 
TCTGCCCGAG CAAAGAAGGC CTTCCGAGAA GGGTCCAGAA AGTCCTCCAG AGTAAAGGGT 
GCAGCGCCAG AGCAGGAGCC AACAGAGTCC CTGTGGGAGA ACAAGATCAA GACGTGGATG 
GAGCGTTATG AGGAGGCCAG CAGCAATCAG TACAGCGAGG ATGTCCAGGT CCTGTTGCGT 
GTCAAAGAAC AAGGCGATGG CAAGAGCCTG GCGTACAACA CGCACCCTGC CTCTTTCAAA 
CCCCCTGTGG AGAGTCAAGT TCAGAAGAAC AAGAAGATCC TCAAAGCGGT GCGAGACTTG 
GCCCCAGACT CCCTCATCAT TGAGTACAGG GGCAAGTTCA TGCTCAGACA GCAGTTTGAG 
GCCAACGGAT ACTTCTTTAA GAGGCCATAC CCATTTGTGT TATTTTATTC TAAATTTGAC 
GGACTGGAGA TGTGCGTGGA CGCTCGTAGC TTCGGCAACG AAGCGCGCTT CATCCGTCGC 
TCCTGCACCC CTAATTCTGA AGTGCGGCAC GTCATTGAGG ACGGCATGCT GCATTTATAC 
ATCTACTCAT TGAGGCCGAT CACCAAGGGC ACAGAGATCA CCATCGGTTT TGATTTTGAC 
TATGGCAGCT GTAAATACAA GGTGGACTGT GCCTGCGTGA AGGGGAATCA GGAGTGCCCG 
GTGCTCAAGC ACAACCTCGA ACCCACAGAG AACCTGGTCG GCCGGCGCAG AGGGAGTCGT 
AAGGACAAGG AGACGGTTCG AGACGACCTG GGCCAGAACC AGAACATGGG TCTGGACGGC 
GAGGGGAAGG GCAAGAGCGT GGGCGACGGC AAACAGAGGA AGCTCTCTCC TCTTCGCCTC 
TCTATCTCAA ACAACCAGGC CAACCCAGCG GAGCAGTCCC ATCTCCCTGT CGGGGCGGCT 
TCCAGCTGGA AAGAACTGAA ACACAAGGAG ACACGAGAGG AGAGGAAGAT GGAGGCCATC 
CTGCAAGCGT TTGCCCGAAT GGAGAAGAGA GAGAAGCGAA GGGAGCAGGC GCTGGAAAGA 
ATCGGTAGCA TCAAGTCGGA GGTCGGGGGA CGCAGTGACA TCAAGGAGGA GCCTCCAGCC 
ACGCCAGAGA CGGCAGATTC TCCAGCTGTT ATGCAGCCGC TCCTGGAGGT GAAAGAGGAG 
CCAGGATTAA AGCCGGCCAA GGTCAAAGGC TCGAGGAACA GGAAAAGTTT CTCAAGGAAC 
CGCACGCACA TTGGCCAACA GCGCCGGAGA GCTCGCACCA TCAGCACCTG CTCTGACCTG 
CAGCCTGGCT CTCCAATTGA ATCTGTGGAG CCTCTGAGCA ACGAAGCCCC CGAAGGAGAG 
ACACCCACCG CACCAGAGCC GGAGGCAATC TCTTCCCAAG CACCAGACAC CAGCCCTCCG 
CACAGCAGCT CACCTGCACC TGACAGAAAC CGCACCGGGA GCAAGAACTT CAAAACTAAA 
AAGCACTTTG TGAGTGAGTG GGTGGGAGAG AAGCAGCAGG ACCGTGGTGC AGTACGAACC 
CCAGAGCCGG CCCCCGAGAG GCCGCTGAGA ATAAGCAGTG ACCCCGAAGT ACTCGCCACA 
CAGCTGAACG CCCTCCCAGG CATGGCCTGC TCTCCACAGG TCTACAGCAC GCCCAAACAC 
TACGTCCGCT TCTCGTCCCC TTTCCTGGCG AACCGCAGTC CCACAACTCC CGGAGTCCCC 
ACTGGGAGAC GGCGTTCCAG AGAACTGCCA GAAACGCCGC CAACCACCGG CTCCTGCAAG 
AAGCGATGGT TGAAGCAGGC CCTTGAGGAA GAGGGATCCA CCAGCCCAGC CAGACGACCG 
AGCCTCCTCA TGCCCTGTGA GGGTCCTCTT AGCCCCTCCA TCAACGGAGA CTCTGACAGC 
CCTCTTCCCT ACAATGGCAC CTGCTCGCTA CCTGAGTTGC CCACGCCTTT GAAAAAGCGC 
CGGTTGAGTC CATTAGATGC CTGTATGTCT GAGAGCTCCA CACCCTACGG CTCCCCTTGT 
GCCACGCCCA CCAGGGCAGA CCAATCGGAG ACACCCGCAA CCCCCATCTT ACTGGCTACC 
CCACCGCGTC CCAGAACGGA GGAGCCCAGT ACAGAGCCCC CGCCCAACAC CCCAACGCAG 
ACACTTAACG CCCCTCTGGA GAGTGAATCC TCTGTGGAGA GCTCCCCAGA GCTCAGCCGG 
AAACCCAGTG TACAAGAGGC CGATCGTCCT CCTTCGTTGG TCTCCTCTCC CTGCATCAGG 
GCTCCCAGTT CAGACGGACT CCCGGCTGAA GCCAAAGTGT CGGTTCCTGA GAGTCCACAG 
CCTCCGGTCG CCGACTCTGT GGACTGTGGA GAGGACAGGA CCGATGGTGG GGTTGTAGAA 
GGCAGTAGTG AGCCTTCCTC GTCCGCAGAA ACATGTGCTT CCTCTTTTCC TGGCTGGATA 
AAAAGCCCAG ACAGAGGCCC GACTGGACCA GCTGGCCTGA ACTTCTCTCC AGTCAACTCA 
AACCTAAGGG ACCTCACCCC CTCACACACT CTGGAGCCCC TGGCGGCTCC CTTCAGGCCC 
GACGCTGCAG CTGGGGCTGC GGCAGGGTCC ACAGCAGGGA CCGGGTCATT GGTTGGCCCT 
CAAGCCCCCT TCAGTGAAGG CCAGGGGCAG CTGTTTTACC CCTGCTCTGA GGAGGGAGGT 
TCACTCGGCT TCTCGCGCTC ACTGAATGGG GATGGCAGCG GTGAAGGAGG AGGATCAGCG 
CAGAACCCCC CACAGAAGAA AAAGGTTTCC CTGCTAGAAT ACAGGAAACG TCAGCGCGAA 
GCCCGTCGCA GCGGCTCCAA GACCGAGTGC AGCTCTCCCG TCTCCACCGT GCCACCTCTG 
ACCGTGGACG CCTTCCCCGT CGCGCTGGAG ACGACAAGCG AACCTCCACT ACCCCCTGCT 
CCTACGGCTG TCTGCAACGC CAACAACACC ATCGTTGTTA AAGAGCCCCA GACGAGCGAG 
GAGGCCGAGA CGCCCGGAGA GAAAGGCGAG AAGGAAGGAG GAGAAGGACA GTGGACGTCG 
TCCACTTCTG TAGAGCAGGC CCGAGAACGC AGCTACCACA GAGCTCTGCT GCTCAGCAAA 
GACAAGGACA CGGATGGTGA GACAGAAGGT GGAGATACAC CTGCACTGAG AGATTGCCCG 
TCTCCAAGTC TTCAGAAGAC CCCGACTCAC ACCCCCTGCT CTCCTGGTCC TCTCGCTCAG 
CCCACCAATC GGCCACCGAA GGAGGAAGAC GGCGACGGGC AGCCGAGCAA GCCTGCGGGA 
CCCAAGTCGG CTCCTCTGAC CCCCACAAAG CTGCATCCCA CCCCGTTACC CTCCTCGCCG 
GTCCACTATC CGGGACCCTC CCTCCTGCAC TCGCCCAAGC CTCAGCCTCA GGGCTCGCCG 
TACCGCAGCC AGAGGGCGCT GTTCTCCGCT CAGCCTCAAA ACCAACCGCA GCCCCAGGCT 
CAGACTGGCC CAGCTCCTTT CCCTCAGTAT AACACACAGA GTGCTCCACC GCCACCGCCC 
CCTCCTCCAC CAGCACCACC GGCCTCCACG GCGTATTTTC CCAGCCAGAG CCCCTCGCCG 
GCTGGACCTT TCCCCGGGTT CAAACCCGCC GTCACGCCCC CTTACCCTCC GTGTTCTCAG 
CCCCTGATGC AGACTCTTCC CCACAGCGTG CACTATCAGA GCTCAGCCGC TCCACCGCCG 
CCTCCTCCTC CGCCCCCGCA CCCGATGCCC GGCCCCACCC TGCTGCACGT CAACCTGCAG 
CCCCCTCCCA TCCAGCAGCA CCAGCTCATG CTGACCACCG CCCCTCCGCC GCCTCCTCCC 
CCACAGGGCC AGACCTCACA GCAGCAGCAG CAGCAGCCTC CCGCTGGCAG CTCCCTGCTG 
TCGCTCACCC CTCCTCCGCC GCCTCCTCCA CCTCCTCCAG CCCCCTCGAC CAACGCCCCG 
ATGCAGGCCC ACCACTTTCA GAACTTAGGG GGTTTTCAGC CGGCGTTGCT ACACCCGGGC 
GGCGCCGCCA CAAACCCTTC AGTGCCCCAA TCCACGTCCC CCCCACCCCT TCAGCAGACC 
GGACTGCCTC CACCTCCCCC TCCTCCTCCC CAACAGACTC AACAAGGCCA GGCTCAGGCC 
GCCGCCTCCC AGATGCCTTC TGGGACTCGT GGAGCTCCTG CGTCCTCGAC CCCCTTCCAC 
AGCTCGGGGT ACCTGAGCAC AGGGTGGCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kmt2e ( XM_010738913.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010737215.2 Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X8, mRNA.

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CloneID	OLa112880
Clone ID Related Accession (Same CDS sequence)	XM_010738913.2
Accession Version	XM_010738913.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	histone-lysine N-methyltransferase 2E isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017609671.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 29, 2016 this sequence version replaced XM_010738913.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACAGCAGACA CCTCATACCT GGACATGGCT 
GCGGGCTCAG ACAGACCAGA ATCGGTGGAG GCCAGCCCTG TGGTGGTGGA AAAGTCCAGC 
TACCCACACC AGATCTACAG CAGCAGTTCT CACCATTCCC ACAGTTACAT TGGCCTGCCT 
TACGCTGACC ATAACTATGG GGCGCGGCCC CCACCTACAC CACCAGCCTC CCCTCCCCCC 
TCCATGTTGA TCCGACAAGG AGAGGGGGGG TTGTTTGTTC CAGGGGGCCA GGACGAAGCA 
TCAAGGGGCA CGACGCTCAG CACCTCAGAG GATGGCAGCT ACGGGGCTGA CATCACCCGC 
TGCATCTGTG GCTTCACCCA CGATGACGGC TACATGATCT GCTGTGATAA GTGCAGTGCC 
TGGCAGCATA TAGACTGCAT GGGCATCGAC AGGCAGAACA TTCCTGAGAC CTACCTGTGT 
GAACGCTGCC AACCACGACA CCTGGATCGA GAGAGGGCCA TCCTGCTGCA GACCAGGAAA 
CGGGAATGTT TGTCTGATGG GGACACGAGT GCTACAGAGA GTGGAGATGA GGTGCCGCTA 
GAGCTCTACT CCACCTTCCA ACACACGCCT ACCACCATCA CCCTGACTAC CGCGCGCCTT 
GGCAATAAGC AGGCTGATAA GAAACGCAAA AAGAGCGGTG ATAAAGAGCC TCCAGCGTCT 
TCTGCCCGAG CAAAGAAGGC CTTCCGAGAA GGGTCCAGAA AGTCCTCCAG AGTAAAGGGT 
GCAGCGCCAG AGCAGGAGCC AACAGAGTCC CTGTGGGAGA ACAAGATCAA GACGTGGATG 
GAGCGTTATG AGGAGGCCAG CAGCAATCAG TACAGCGAGG ATGTCCAGGT CCTGTTGCGT 
GTCAAAGAAC AAGGCGATGG CAAGAGCCTG GCGTACAACA CGCACCCTGC CTCTTTCAAA 
CCCCCTGTGG AGAGTCAAGT TCAGAAGAAC AAGAAGATCC TCAAAGCGGT GCGAGACTTG 
GCCCCAGACT CCCTCATCAT TGAGTACAGG GGCAAGTTCA TGCTCAGACA GCAGTTTGAG 
GCCAACGGAT ACTTCTTTAA GAGGCCATAC CCATTTGTGT TATTTTATTC TAAATTTGAC 
GGACTGGAGA TGTGCGTGGA CGCTCGTAGC TTCGGCAACG AAGCGCGCTT CATCCGTCGC 
TCCTGCACCC CTAATTCTGA AGTGCGGCAC GTCATTGAGG ACGGCATGCT GCATTTATAC 
ATCTACTCAT TGAGGCCGAT CACCAAGGGC ACAGAGATCA CCATCGGTTT TGATTTTGAC 
TATGGCAGCT GTAAATACAA GGTGGACTGT GCCTGCGTGA AGGGGAATCA GGAGTGCCCG 
GTGCTCAAGC ACAACCTCGA ACCCACAGAG AACCTGGTCG GCCGGCGCAG AGGGAGTCGT 
AAGGACAAGG AGACGGTTCG AGACGACCTG GGCCAGAACC AGAACATGGG TCTGGACGGC 
GAGGGGAAGG GCAAGAGCGT GGGCGACGGC AAACAGAGGA AGCTCTCTCC TCTTCGCCTC 
TCTATCTCAA ACAACCAGAC ACGAGAGGAG AGGAAGATGG AGGCCATCCT GCAAGCGTTT 
GCCCGAATGG AGAAGAGAGA GAAGCGAAGG GAGCAGGCGC TGGAAAGAAT CGGTAGCATC 
AAGTCGGAGG TCGGGGGACG CAGTGACATC AAGGAGGAGC CTCCAGCCAC GCCAGAGACG 
GCAGATTCTC CAGCTGTTAT GCAGCCGCTC CTGGAGGTGA AAGAGGAGCC AGGATTAAAG 
CCGGCCAAGG TCAAAGGCTC GAGGAACAGG AAAAGTTTCT CAAGGAACCG CACGCACATT 
GGCCAACAGC GCCGGAGAGC TCGCACCATC AGCACCTGCT CTGACCTGCA GCCTGGCTCT 
CCAATTGAAT CTGTGGAGCC TCTGAGCAAC GAAGCCCCCG AAGGAGAGAC ACCCACCGCA 
CCAGAGCCGG AGGCAATCTC TTCCCAAGCA CCAGACACCA GCCCTCCGCA CAGCAGCTCA 
CCTGCACCTG ACAGAAACCG CACCGGGAGC AAGAACTTCA AAACTAAAAA GCACTTTGTG 
AGTGAGTGGG TGGGAGAGAA GCAGCAGGAC CGTGGTGCAG TACGAACCCC AGAGCCGGCC 
CCCGAGAGGC CGCTGAGAAT AAGCAGTGAC CCCGAAGTAC TCGCCACACA GCTGAACGCC 
CTCCCAGGCA TGGCCTGCTC TCCACAGGTC TACAGCACGC CCAAACACTA CGTCCGCTTC 
TCGTCCCCTT TCCTGGCGAA CCGCAGTCCC ACAACTCCCG GAGTCCCCAC TGGGAGACGG 
CGTTCCAGAG AACTGCCAGA AACGCCGCCA ACCACCGGCT CCTGCAAGAA GCGATGGTTG 
AAGCAGGCCC TTGAGGAAGA GGGATCCACC AGCCCAGCCA GACGACCGAG CCTCCTCATG 
CCCTGTGAGG GTCCTCTTAG CCCCTCCATC AACGGAGACT CTGACAGCCC TCTTCCCTAC 
AATGGCACCT GCTCGCTACC TGAGTTGCCC ACGCCTTTGA AAAAGCGCCG GTTGAGTCCA 
TTAGATGCCT GTATGTCTGA GAGCTCCACA CCCTACGGCT CCCCTTGTGC CACGCCCACC 
AGGGCAGACC AATCGGAGAC ACCCGCAACC CCCATCTTAC TGGCTACCCC ACCGCGTCCC 
AGAACGGAGG AGCCCAGTAC AGAGCCCCCG CCCAACACCC CAACGCAGAC ACTTAACGCC 
CCTCTGGAGA GTGAATCCTC TGTGGAGAGC TCCCCAGAGC TCAGCCGGAA ACCCAGTGTA 
CAAGAGGCCG ATCGTCCTCC TTCGTTGGTC TCCTCTCCCT GCATCAGGGC TCCCAGTTCA 
GACGGACTCC CGGCTGAAGC CAAAGTGTCG GTTCCTGAGA GTCCACAGCC TCCGGTCGCC 
GACTCTGTGG ACTGTGGAGA GGACAGGACC GATGGTGGGG TTGTAGAAGG CAGTAGTGAG 
CCTTCCTCGT CCGCAGAAAC ATGTGCTTCC TCTTTTCCTG GCTGGATAAA AAGCCCAGAC 
AGAGGCCCGA CTGGACCAGC TGGCCTGAAC TTCTCTCCAG TCAACTCAAA CCTAAGGGAC 
CTCACCCCCT CACACACTCT GGAGCCCCTG GCGGCTCCCT TCAGGCCCGA CGCTGCAGCT 
GGGGCTGCGG CAGGGTCCAC AGCAGGGACC GGGTCATTGG TTGGCCCTCA AGCCCCCTTC 
AGTGAAGGCC AGGGGCAGCT GTTTTACCCC TGCTCTGAGG AGGGAGGTTC ACTCGGCTTC 
TCGCGCTCAC TGAATGGGGA TGGCAGCGGT GAAGGAGGAG GATCAGCGCA GAACCCCCCA 
CAGAAGAAAA AGGTTTCCCT GCTAGAATAC AGGAAACGTC AGCGCGAAGC CCGTCGCAGC 
GGCTCCAAGA CCGAGTGCAG CTCTCCCGTC TCCACCGTGC CACCTCTGAC CGTGGACGCC 
TTCCCCGTCG CGCTGGAGAC GACAAGCGAA CCTCCACTAC CCCCTGCTCC TACGGCTGTC 
TGCAACGCCA ACAACACCAT CGTTGTTAAA GAGCCCCAGA CGAGCGAGGA GGCCGAGACG 
CCCGGAGAGA AAGGCGAGAA GGAAGGAGGA GAAGGACAGT GGACGTCGTC CACTTCTGTA 
GAGCAGGCCC GAGAACGCAG CTACCACAGA GCTCTGCTGC TCAGCAAAGA CAAGGACACG 
GATGGTGAGA CAGAAGGTGG AGATACACCT GCACTGAGAG ATTGCCCGTC TCCAAGTCTT 
CAGAAGACCC CGACTCACAC CCCCTGCTCT CCTGGTCCTC TCGCTCAGCC CACCAATCGG 
CCACCGAAGG AGGAAGACGG CGACGGGCAG CCGAGCAAGC CTGCGGGACC CAAGTCGGCT 
CCTCTGACCC CCACAAAGCT GCATCCCACC CCGTTACCCT CCTCGCCGGT CCACTATCCG 
GGACCCTCCC TCCTGCACTC GCCCAAGCCT CAGCCTCAGG GCTCGCCGTA CCGCAGCCAG 
AGGGCGCTGT TCTCCGCTCA GCCTCAAAAC CAACCGCAGC CCCAGGCTCA GACTGGCCCA 
GCTCCTTTCC CTCAGTATAA CACACAGAGT GCTCCACCGC CACCGCCCCC TCCTCCACCA 
GCACCACCGG CCTCCACGGC GTATTTTCCC AGCCAGAGCC CCTCGCCGGC TGGACCTTTC 
CCCGGGTTCA AACCCGCCGT CACGCCCCCT TACCCTCCGT GTTCTCAGCC CCTGATGCAG 
ACTCTTCCCC ACAGCGTGCA CTATCAGAGC TCAGCCGCTC CACCGCCGCC TCCTCCTCCG 
CCCCCGCACC CGATGCCCGG CCCCACCCTG CTGCACGTCA ACCTGCAGCC CCCTCCCATC 
CAGCAGCACC AGCTCATGCT GACCACCGCC CCTCCGCCGC CTCCTCCCCC ACAGGGCCAG 
ACCTCACAGC AGCAGCAGCA GCAGCCTCCC GCTGGCAGCT CCCTGCTGTC GCTCACCCCT 
CCTCCGCCGC CTCCTCCACC TCCTCCAGCC CCCTCGACCA ACGCCCCGAT GCAGGCCCAC 
CACTTTCAGA ACTTAGGGGG TTTTCAGCCG GCGTTGCTAC ACCCGGGCGG CGCCGCCACA 
AACCCTTCAG TGCCCCAATC CACGTCCCCC CCACCCCTTC AGCAGACCGG ACTGCCTCCA 
CCTCCCCCTC CTCCTCCCCA ACAGACTCAA CAAGGCCAGG CTCAGGCCGC CGCCTCCCAG 
ATGCCTTCTG GGACTCGTGG AGCTCCTGCG TCCTCGACCC CCTTCCACAG CTCGGGGTAC 
CTGAGCACAG GGTGGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010737215.2
CDS	492..5312
Translation	MSIVIPVGVDTADTSYLDMAAGSDRPESVEASPVVVEKSSYPHQIYSSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPSMLIRQGEGGLFVPGGQDEASRGTTLSTSEDGSYGADITRCICGFTHDDGYMICCDKCSAWQHIDCMGIDRQNIPETYLCERCQPRHLDRERAILLQTRKRECLSDGDTSATESGDEVPLELYSTFQHTPTTITLTTARLGNKQADKKRKKSGDKEPPASSARAKKAFREGSRKSSRVKGAAPEQEPTESLWENKIKTWMERYEEASSNQYSEDVQVLLRVKEQGDGKSLAYNTHPASFKPPVESQVQKNKKILKAVRDLAPDSLIIEYRGKFMLRQQFEANGYFFKRPYPFVLFYSKFDGLEMCVDARSFGNEARFIRRSCTPNSEVRHVIEDGMLHLYIYSLRPITKGTEITIGFDFDYGSCKYKVDCACVKGNQECPVLKHNLEPTENLVGRRRGSRKDKETVRDDLGQNQNMGLDGEGKGKSVGDGKQRKLSPLRLSISNNQTREERKMEAILQAFARMEKREKRREQALERIGSIKSEVGGRSDIKEEPPATPETADSPAVMQPLLEVKEEPGLKPAKVKGSRNRKSFSRNRTHIGQQRRRARTISTCSDLQPGSPIESVEPLSNEAPEGETPTAPEPEAISSQAPDTSPPHSSSPAPDRNRTGSKNFKTKKHFVSEWVGEKQQDRGAVRTPEPAPERPLRISSDPEVLATQLNALPGMACSPQVYSTPKHYVRFSSPFLANRSPTTPGVPTGRRRSRELPETPPTTGSCKKRWLKQALEEEGSTSPARRPSLLMPCEGPLSPSINGDSDSPLPYNGTCSLPELPTPLKKRRLSPLDACMSESSTPYGSPCATPTRADQSETPATPILLATPPRPRTEEPSTEPPPNTPTQTLNAPLESESSVESSPELSRKPSVQEADRPPSLVSSPCIRAPSSDGLPAEAKVSVPESPQPPVADSVDCGEDRTDGGVVEGSSEPSSSAETCASSFPGWIKSPDRGPTGPAGLNFSPVNSNLRDLTPSHTLEPLAAPFRPDAAAGAAAGSTAGTGSLVGPQAPFSEGQGQLFYPCSEEGGSLGFSRSLNGDGSGEGGGSAQNPPQKKKVSLLEYRKRQREARRSGSKTECSSPVSTVPPLTVDAFPVALETTSEPPLPPAPTAVCNANNTIVVKEPQTSEEAETPGEKGEKEGGEGQWTSSTSVEQARERSYHRALLLSKDKDTDGETEGGDTPALRDCPSPSLQKTPTHTPCSPGPLAQPTNRPPKEEDGDGQPSKPAGPKSAPLTPTKLHPTPLPSSPVHYPGPSLLHSPKPQPQGSPYRSQRALFSAQPQNQPQPQAQTGPAPFPQYNTQSAPPPPPPPPPAPPASTAYFPSQSPSPAGPFPGFKPAVTPPYPPCSQPLMQTLPHSVHYQSSAAPPPPPPPPPHPMPGPTLLHVNLQPPPIQQHQLMLTTAPPPPPPPQGQTSQQQQQQPPAGSSLLSLTPPPPPPPPPPAPSTNAPMQAHHFQNLGGFQPALLHPGGAATNPSVPQSTSPPPLQQTGLPPPPPPPPQQTQQGQAQAAASQMPSGTRGAPASSTPFHSSGYLSTGWH

Target ORF information:

RefSeq Version	XM_010738913.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea lysine methyltransferase 2E (kmt2e), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010738913.2

ORF Insert Sequence:

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACAGCAGACA CCTCATACCT GGACATGGCT 
GCGGGCTCAG ACAGACCAGA ATCGGTGGAG GCCAGCCCTG TGGTGGTGGA AAAGTCCAGC 
TACCCACACC AGATCTACAG CAGCAGTTCT CACCATTCCC ACAGTTACAT TGGCCTGCCT 
TACGCTGACC ATAACTATGG GGCGCGGCCC CCACCTACAC CACCAGCCTC CCCTCCCCCC 
TCCATGTTGA TCCGACAAGG AGAGGGGGGG TTGTTTGTTC CAGGGGGCCA GGACGAAGCA 
TCAAGGGGCA CGACGCTCAG CACCTCAGAG GATGGCAGCT ACGGGGCTGA CATCACCCGC 
TGCATCTGTG GCTTCACCCA CGATGACGGC TACATGATCT GCTGTGATAA GTGCAGTGCC 
TGGCAGCATA TAGACTGCAT GGGCATCGAC AGGCAGAACA TTCCTGAGAC CTACCTGTGT 
GAACGCTGCC AACCACGACA CCTGGATCGA GAGAGGGCCA TCCTGCTGCA GACCAGGAAA 
CGGGAATGTT TGTCTGATGG GGACACGAGT GCTACAGAGA GTGGAGATGA GGTGCCGCTA 
GAGCTCTACT CCACCTTCCA ACACACGCCT ACCACCATCA CCCTGACTAC CGCGCGCCTT 
GGCAATAAGC AGGCTGATAA GAAACGCAAA AAGAGCGGTG ATAAAGAGCC TCCAGCGTCT 
TCTGCCCGAG CAAAGAAGGC CTTCCGAGAA GGGTCCAGAA AGTCCTCCAG AGTAAAGGGT 
GCAGCGCCAG AGCAGGAGCC AACAGAGTCC CTGTGGGAGA ACAAGATCAA GACGTGGATG 
GAGCGTTATG AGGAGGCCAG CAGCAATCAG TACAGCGAGG ATGTCCAGGT CCTGTTGCGT 
GTCAAAGAAC AAGGCGATGG CAAGAGCCTG GCGTACAACA CGCACCCTGC CTCTTTCAAA 
CCCCCTGTGG AGAGTCAAGT TCAGAAGAAC AAGAAGATCC TCAAAGCGGT GCGAGACTTG 
GCCCCAGACT CCCTCATCAT TGAGTACAGG GGCAAGTTCA TGCTCAGACA GCAGTTTGAG 
GCCAACGGAT ACTTCTTTAA GAGGCCATAC CCATTTGTGT TATTTTATTC TAAATTTGAC 
GGACTGGAGA TGTGCGTGGA CGCTCGTAGC TTCGGCAACG AAGCGCGCTT CATCCGTCGC 
TCCTGCACCC CTAATTCTGA AGTGCGGCAC GTCATTGAGG ACGGCATGCT GCATTTATAC 
ATCTACTCAT TGAGGCCGAT CACCAAGGGC ACAGAGATCA CCATCGGTTT TGATTTTGAC 
TATGGCAGCT GTAAATACAA GGTGGACTGT GCCTGCGTGA AGGGGAATCA GGAGTGCCCG 
GTGCTCAAGC ACAACCTCGA ACCCACAGAG AACCTGGTCG GCCGGCGCAG AGGGAGTCGT 
AAGGACAAGG AGACGGTTCG AGACGACCTG GGCCAGAACC AGAACATGGG TCTGGACGGC 
GAGGGGAAGG GCAAGAGCGT GGGCGACGGC AAACAGAGGA AGCTCTCTCC TCTTCGCCTC 
TCTATCTCAA ACAACCAGAC ACGAGAGGAG AGGAAGATGG AGGCCATCCT GCAAGCGTTT 
GCCCGAATGG AGAAGAGAGA GAAGCGAAGG GAGCAGGCGC TGGAAAGAAT CGGTAGCATC 
AAGTCGGAGG TCGGGGGACG CAGTGACATC AAGGAGGAGC CTCCAGCCAC GCCAGAGACG 
GCAGATTCTC CAGCTGTTAT GCAGCCGCTC CTGGAGGTGA AAGAGGAGCC AGGATTAAAG 
CCGGCCAAGG TCAAAGGCTC GAGGAACAGG AAAAGTTTCT CAAGGAACCG CACGCACATT 
GGCCAACAGC GCCGGAGAGC TCGCACCATC AGCACCTGCT CTGACCTGCA GCCTGGCTCT 
CCAATTGAAT CTGTGGAGCC TCTGAGCAAC GAAGCCCCCG AAGGAGAGAC ACCCACCGCA 
CCAGAGCCGG AGGCAATCTC TTCCCAAGCA CCAGACACCA GCCCTCCGCA CAGCAGCTCA 
CCTGCACCTG ACAGAAACCG CACCGGGAGC AAGAACTTCA AAACTAAAAA GCACTTTGTG 
AGTGAGTGGG TGGGAGAGAA GCAGCAGGAC CGTGGTGCAG TACGAACCCC AGAGCCGGCC 
CCCGAGAGGC CGCTGAGAAT AAGCAGTGAC CCCGAAGTAC TCGCCACACA GCTGAACGCC 
CTCCCAGGCA TGGCCTGCTC TCCACAGGTC TACAGCACGC CCAAACACTA CGTCCGCTTC 
TCGTCCCCTT TCCTGGCGAA CCGCAGTCCC ACAACTCCCG GAGTCCCCAC TGGGAGACGG 
CGTTCCAGAG AACTGCCAGA AACGCCGCCA ACCACCGGCT CCTGCAAGAA GCGATGGTTG 
AAGCAGGCCC TTGAGGAAGA GGGATCCACC AGCCCAGCCA GACGACCGAG CCTCCTCATG 
CCCTGTGAGG GTCCTCTTAG CCCCTCCATC AACGGAGACT CTGACAGCCC TCTTCCCTAC 
AATGGCACCT GCTCGCTACC TGAGTTGCCC ACGCCTTTGA AAAAGCGCCG GTTGAGTCCA 
TTAGATGCCT GTATGTCTGA GAGCTCCACA CCCTACGGCT CCCCTTGTGC CACGCCCACC 
AGGGCAGACC AATCGGAGAC ACCCGCAACC CCCATCTTAC TGGCTACCCC ACCGCGTCCC 
AGAACGGAGG AGCCCAGTAC AGAGCCCCCG CCCAACACCC CAACGCAGAC ACTTAACGCC 
CCTCTGGAGA GTGAATCCTC TGTGGAGAGC TCCCCAGAGC TCAGCCGGAA ACCCAGTGTA 
CAAGAGGCCG ATCGTCCTCC TTCGTTGGTC TCCTCTCCCT GCATCAGGGC TCCCAGTTCA 
GACGGACTCC CGGCTGAAGC CAAAGTGTCG GTTCCTGAGA GTCCACAGCC TCCGGTCGCC 
GACTCTGTGG ACTGTGGAGA GGACAGGACC GATGGTGGGG TTGTAGAAGG CAGTAGTGAG 
CCTTCCTCGT CCGCAGAAAC ATGTGCTTCC TCTTTTCCTG GCTGGATAAA AAGCCCAGAC 
AGAGGCCCGA CTGGACCAGC TGGCCTGAAC TTCTCTCCAG TCAACTCAAA CCTAAGGGAC 
CTCACCCCCT CACACACTCT GGAGCCCCTG GCGGCTCCCT TCAGGCCCGA CGCTGCAGCT 
GGGGCTGCGG CAGGGTCCAC AGCAGGGACC GGGTCATTGG TTGGCCCTCA AGCCCCCTTC 
AGTGAAGGCC AGGGGCAGCT GTTTTACCCC TGCTCTGAGG AGGGAGGTTC ACTCGGCTTC 
TCGCGCTCAC TGAATGGGGA TGGCAGCGGT GAAGGAGGAG GATCAGCGCA GAACCCCCCA 
CAGAAGAAAA AGGTTTCCCT GCTAGAATAC AGGAAACGTC AGCGCGAAGC CCGTCGCAGC 
GGCTCCAAGA CCGAGTGCAG CTCTCCCGTC TCCACCGTGC CACCTCTGAC CGTGGACGCC 
TTCCCCGTCG CGCTGGAGAC GACAAGCGAA CCTCCACTAC CCCCTGCTCC TACGGCTGTC 
TGCAACGCCA ACAACACCAT CGTTGTTAAA GAGCCCCAGA CGAGCGAGGA GGCCGAGACG 
CCCGGAGAGA AAGGCGAGAA GGAAGGAGGA GAAGGACAGT GGACGTCGTC CACTTCTGTA 
GAGCAGGCCC GAGAACGCAG CTACCACAGA GCTCTGCTGC TCAGCAAAGA CAAGGACACG 
GATGGTGAGA CAGAAGGTGG AGATACACCT GCACTGAGAG ATTGCCCGTC TCCAAGTCTT 
CAGAAGACCC CGACTCACAC CCCCTGCTCT CCTGGTCCTC TCGCTCAGCC CACCAATCGG 
CCACCGAAGG AGGAAGACGG CGACGGGCAG CCGAGCAAGC CTGCGGGACC CAAGTCGGCT 
CCTCTGACCC CCACAAAGCT GCATCCCACC CCGTTACCCT CCTCGCCGGT CCACTATCCG 
GGACCCTCCC TCCTGCACTC GCCCAAGCCT CAGCCTCAGG GCTCGCCGTA CCGCAGCCAG 
AGGGCGCTGT TCTCCGCTCA GCCTCAAAAC CAACCGCAGC CCCAGGCTCA GACTGGCCCA 
GCTCCTTTCC CTCAGTATAA CACACAGAGT GCTCCACCGC CACCGCCCCC TCCTCCACCA 
GCACCACCGG CCTCCACGGC GTATTTTCCC AGCCAGAGCC CCTCGCCGGC TGGACCTTTC 
CCCGGGTTCA AACCCGCCGT CACGCCCCCT TACCCTCCGT GTTCTCAGCC CCTGATGCAG 
ACTCTTCCCC ACAGCGTGCA CTATCAGAGC TCAGCCGCTC CACCGCCGCC TCCTCCTCCG 
CCCCCGCACC CGATGCCCGG CCCCACCCTG CTGCACGTCA ACCTGCAGCC CCCTCCCATC 
CAGCAGCACC AGCTCATGCT GACCACCGCC CCTCCGCCGC CTCCTCCCCC ACAGGGCCAG 
ACCTCACAGC AGCAGCAGCA GCAGCCTCCC GCTGGCAGCT CCCTGCTGTC GCTCACCCCT 
CCTCCGCCGC CTCCTCCACC TCCTCCAGCC CCCTCGACCA ACGCCCCGAT GCAGGCCCAC 
CACTTTCAGA ACTTAGGGGG TTTTCAGCCG GCGTTGCTAC ACCCGGGCGG CGCCGCCACA 
AACCCTTCAG TGCCCCAATC CACGTCCCCC CCACCCCTTC AGCAGACCGG ACTGCCTCCA 
CCTCCCCCTC CTCCTCCCCA ACAGACTCAA CAAGGCCAGG CTCAGGCCGC CGCCTCCCAG 
ATGCCTTCTG GGACTCGTGG AGCTCCTGCG TCCTCGACCC CCTTCCACAG CTCGGGGTAC 
CTGAGCACAG GGTGGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	kmt2e
Entrez Gene ID	104925314
Full Name	lysine methyltransferase 2E
Gene Type	protein-coding
Organism	Larimichthys crocea(large yellow croaker)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

kmt2e cDNA ORF clone, Larimichthys crocea(large yellow croaker)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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